LOCUS       DASHFF010000001       114163 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.001, whole genome shotgun sequence.
ACCESSION   DASHFF010000001 DASHFF010000000
VERSION     DASHFF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 114163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.001"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            172..444
                     /locus_tag="U2I75_000001"
     CDS             172..444
                     /locus_tag="U2I75_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695171.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            459..1382
                     /locus_tag="U2I75_000002"
     CDS             459..1382
                     /locus_tag="U2I75_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1695172.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1522..2274)
                     /locus_tag="U2I75_000003"
     CDS             complement(1522..2274)
                     /locus_tag="U2I75_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1695173.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            2497..2859
                     /locus_tag="U2I75_000004"
     CDS             2497..2859
                     /locus_tag="U2I75_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN1695174.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3496..4968
                     /locus_tag="U2I75_000005"
     CDS             3496..4968
                     /locus_tag="U2I75_000005"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN1695175.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            4980..5804
                     /gene="nadE"
                     /locus_tag="U2I75_000006"
     CDS             4980..5804
                     /gene="nadE"
                     /locus_tag="U2I75_000006"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN1695176.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(5877..6767)
                     /locus_tag="U2I75_000007"
     CDS             complement(5877..6767)
                     /locus_tag="U2I75_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN1695177.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            6965..8482
                     /locus_tag="U2I75_000008"
     CDS             6965..8482
                     /locus_tag="U2I75_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1695178.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8454..9743
                     /locus_tag="U2I75_000009"
     CDS             8454..9743
                     /locus_tag="U2I75_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1695179.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            9918..10814
                     /locus_tag="U2I75_000010"
     CDS             9918..10814
                     /locus_tag="U2I75_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1695180.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            10828..11691
                     /locus_tag="U2I75_000011"
     CDS             10828..11691
                     /locus_tag="U2I75_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1695181.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            11697..12260
                     /locus_tag="U2I75_000012"
     CDS             11697..12260
                     /locus_tag="U2I75_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN1695182.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            12282..13793
                     /locus_tag="U2I75_000013"
     CDS             12282..13793
                     /locus_tag="U2I75_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1695183.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            13805..14764
                     /locus_tag="U2I75_000014"
     CDS             13805..14764
                     /locus_tag="U2I75_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1695184.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            14850..15929
                     /locus_tag="U2I75_000015"
     CDS             14850..15929
                     /locus_tag="U2I75_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1695185.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(16181..17581)
                     /locus_tag="U2I75_000016"
     CDS             complement(16181..17581)
                     /locus_tag="U2I75_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN1695186.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            17799..19460
                     /locus_tag="U2I75_000017"
     CDS             17799..19460
                     /locus_tag="U2I75_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN1695187.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            19417..20115
                     /locus_tag="U2I75_000018"
     CDS             19417..20115
                     /locus_tag="U2I75_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1695188.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            20131..21555
                     /gene="araA"
                     /locus_tag="U2I75_000019"
     CDS             20131..21555
                     /gene="araA"
                     /locus_tag="U2I75_000019"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN1695189.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            21862..23021
                     /locus_tag="U2I75_000020"
                     /pseudo
     CDS             21862..23021
                     /locus_tag="U2I75_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            23139..24998
                     /locus_tag="U2I75_000021"
     CDS             23139..24998
                     /locus_tag="U2I75_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1695190.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            25108..25542
                     /locus_tag="U2I75_000022"
     CDS             25108..25542
                     /locus_tag="U2I75_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695191.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(25801..25962)
                     /locus_tag="U2I75_000023"
     CDS             complement(25801..25962)
                     /locus_tag="U2I75_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695192.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(25981..27003)
                     /locus_tag="U2I75_000024"
     CDS             complement(25981..27003)
                     /locus_tag="U2I75_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN1695193.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            27131..27484
                     /locus_tag="U2I75_000025"
     CDS             27131..27484
                     /locus_tag="U2I75_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1695194.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            27496..28662
                     /locus_tag="U2I75_000026"
     CDS             27496..28662
                     /locus_tag="U2I75_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1695195.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(28754..29374)
                     /locus_tag="U2I75_000027"
     CDS             complement(28754..29374)
                     /locus_tag="U2I75_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN1695196.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(29566..29862)
                     /locus_tag="U2I75_000028"
     CDS             complement(29566..29862)
                     /locus_tag="U2I75_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1695197.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            30066..30716
                     /locus_tag="U2I75_000029"
     CDS             30066..30716
                     /locus_tag="U2I75_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1695198.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            30732..32459
                     /locus_tag="U2I75_000030"
     CDS             30732..32459
                     /locus_tag="U2I75_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695199.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            32456..34348
                     /locus_tag="U2I75_000031"
     CDS             32456..34348
                     /locus_tag="U2I75_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695200.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            35052..35921
                     /locus_tag="U2I75_000032"
     CDS             35052..35921
                     /locus_tag="U2I75_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEN1695201.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            36130..37155
                     /locus_tag="U2I75_000033"
     CDS             36130..37155
                     /locus_tag="U2I75_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1695202.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            37194..38144
                     /gene="manA"
                     /locus_tag="U2I75_000034"
     CDS             37194..38144
                     /gene="manA"
                     /locus_tag="U2I75_000034"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN1695203.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            38238..39941
                     /locus_tag="U2I75_000035"
     CDS             38238..39941
                     /locus_tag="U2I75_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1695204.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            39943..41976
                     /locus_tag="U2I75_000036"
     CDS             39943..41976
                     /locus_tag="U2I75_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1695205.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            41987..42427
                     /locus_tag="U2I75_000037"
     CDS             41987..42427
                     /locus_tag="U2I75_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1695206.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(42627..43754)
                     /locus_tag="U2I75_000038"
     CDS             complement(42627..43754)
                     /locus_tag="U2I75_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695207.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(43757..44434)
                     /locus_tag="U2I75_000039"
     CDS             complement(43757..44434)
                     /locus_tag="U2I75_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695208.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            44698..46464
                     /locus_tag="U2I75_000040"
     CDS             44698..46464
                     /locus_tag="U2I75_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN1695209.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            46479..46916
                     /locus_tag="U2I75_000041"
     CDS             46479..46916
                     /locus_tag="U2I75_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1695210.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            46929..48080
                     /locus_tag="U2I75_000042"
     CDS             46929..48080
                     /locus_tag="U2I75_000042"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN1695211.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(48142..48840)
                     /locus_tag="U2I75_000043"
     CDS             complement(48142..48840)
                     /locus_tag="U2I75_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN1695212.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(48833..50185)
                     /locus_tag="U2I75_000044"
     CDS             complement(48833..50185)
                     /locus_tag="U2I75_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN1695213.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(50408..51808)
                     /locus_tag="U2I75_000045"
     CDS             complement(50408..51808)
                     /locus_tag="U2I75_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN1695214.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            51969..52706
                     /locus_tag="U2I75_000046"
     CDS             51969..52706
                     /locus_tag="U2I75_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN1695215.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            52831..53310
                     /locus_tag="U2I75_000047"
     CDS             52831..53310
                     /locus_tag="U2I75_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN1695216.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            53307..54074
                     /locus_tag="U2I75_000048"
     CDS             53307..54074
                     /locus_tag="U2I75_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695217.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            54304..54873
                     /gene="lepB"
                     /locus_tag="U2I75_000049"
     CDS             54304..54873
                     /gene="lepB"
                     /locus_tag="U2I75_000049"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1695218.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(54977..55579)
                     /locus_tag="U2I75_000050"
     CDS             complement(54977..55579)
                     /locus_tag="U2I75_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1695219.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            55685..56154
                     /locus_tag="U2I75_000051"
                     /pseudo
     CDS             55685..56154
                     /locus_tag="U2I75_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            56260..56844
                     /locus_tag="U2I75_000052"
     CDS             56260..56844
                     /locus_tag="U2I75_000052"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN1695220.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            56847..57920
                     /gene="prfA"
                     /locus_tag="U2I75_000053"
     CDS             56847..57920
                     /gene="prfA"
                     /locus_tag="U2I75_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN1695221.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            57913..58752
                     /gene="prmC"
                     /locus_tag="U2I75_000054"
     CDS             57913..58752
                     /gene="prmC"
                     /locus_tag="U2I75_000054"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN1695222.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            58745..58924
                     /locus_tag="U2I75_000055"
     CDS             58745..58924
                     /locus_tag="U2I75_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695223.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            58946..59971
                     /locus_tag="U2I75_000056"
     CDS             58946..59971
                     /locus_tag="U2I75_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN1695224.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            60146..61390
                     /locus_tag="U2I75_000057"
     CDS             60146..61390
                     /locus_tag="U2I75_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN1695225.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            61543..62490
                     /locus_tag="U2I75_000058"
     CDS             61543..62490
                     /locus_tag="U2I75_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1695226.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            62624..63253
                     /gene="upp"
                     /locus_tag="U2I75_000059"
     CDS             62624..63253
                     /gene="upp"
                     /locus_tag="U2I75_000059"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN1695227.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(63392..63634)
                     /locus_tag="U2I75_000060"
     CDS             complement(63392..63634)
                     /locus_tag="U2I75_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695228.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            63755..63827
                     /locus_tag="U2I75_000061"
     tRNA            63755..63827
                     /locus_tag="U2I75_000061"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63788..63790,aa:Thr,seq:cgt)
     gene            complement(63946..64584)
                     /locus_tag="U2I75_000062"
     CDS             complement(63946..64584)
                     /locus_tag="U2I75_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1695229.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(64707..65339)
                     /locus_tag="U2I75_000063"
     CDS             complement(64707..65339)
                     /locus_tag="U2I75_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1695230.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            65690..65989
                     /locus_tag="U2I75_000064"
     CDS             65690..65989
                     /locus_tag="U2I75_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1695231.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            66484..68649
                     /gene="acm"
                     /locus_tag="U2I75_000065"
     CDS             66484..68649
                     /gene="acm"
                     /locus_tag="U2I75_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN1695232.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(68921..69406)
                     /locus_tag="U2I75_000066"
     CDS             complement(68921..69406)
                     /locus_tag="U2I75_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN1695233.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    69496..69841
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            69886..70527
                     /locus_tag="U2I75_000067"
     CDS             69886..70527
                     /locus_tag="U2I75_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1695234.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            70546..71181
                     /locus_tag="U2I75_000068"
     CDS             70546..71181
                     /locus_tag="U2I75_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1695235.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            71199..72014
                     /locus_tag="U2I75_000069"
     CDS             71199..72014
                     /locus_tag="U2I75_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1695236.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            72333..73769
                     /locus_tag="U2I75_000070"
     CDS             72333..73769
                     /locus_tag="U2I75_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1695237.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            73958..76405
                     /locus_tag="U2I75_000071"
     CDS             73958..76405
                     /locus_tag="U2I75_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN1695238.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            76635..77702
                     /gene="rlmN"
                     /locus_tag="U2I75_000072"
     CDS             76635..77702
                     /gene="rlmN"
                     /locus_tag="U2I75_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN1695239.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(77895..78998)
                     /locus_tag="U2I75_000073"
     CDS             complement(77895..78998)
                     /locus_tag="U2I75_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1695240.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            79551..80099
                     /gene="aac(6')"
                     /locus_tag="U2I75_000074"
     CDS             79551..80099
                     /gene="aac(6')"
                     /locus_tag="U2I75_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1695241.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            80645..82306
                     /locus_tag="U2I75_000075"
     CDS             80645..82306
                     /locus_tag="U2I75_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1695242.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(82376..82723)
                     /locus_tag="U2I75_000076"
     CDS             complement(82376..82723)
                     /locus_tag="U2I75_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN1695243.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            82807..83748
                     /locus_tag="U2I75_000077"
     CDS             82807..83748
                     /locus_tag="U2I75_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695244.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            83749..84792
                     /locus_tag="U2I75_000078"
     CDS             83749..84792
                     /locus_tag="U2I75_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695245.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            84798..85868
                     /locus_tag="U2I75_000079"
     CDS             84798..85868
                     /locus_tag="U2I75_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695246.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            85858..86811
                     /locus_tag="U2I75_000080"
     CDS             85858..86811
                     /locus_tag="U2I75_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1695247.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            86823..87833
                     /locus_tag="U2I75_000081"
     CDS             86823..87833
                     /locus_tag="U2I75_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1695248.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(88118..88939)
                     /locus_tag="U2I75_000082"
     CDS             complement(88118..88939)
                     /locus_tag="U2I75_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1695249.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(89036..89314)
                     /locus_tag="U2I75_000083"
     CDS             complement(89036..89314)
                     /locus_tag="U2I75_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695250.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            89582..91591
                     /gene="metG"
                     /locus_tag="U2I75_000084"
     CDS             89582..91591
                     /gene="metG"
                     /locus_tag="U2I75_000084"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN1695251.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(92070..92657)
                     /locus_tag="U2I75_000085"
     CDS             complement(92070..92657)
                     /locus_tag="U2I75_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695252.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            92832..94154
                     /locus_tag="U2I75_000086"
     CDS             92832..94154
                     /locus_tag="U2I75_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1695253.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            94309..95085
                     /locus_tag="U2I75_000087"
     CDS             94309..95085
                     /locus_tag="U2I75_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1695254.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            95078..95647
                     /gene="rnmV"
                     /locus_tag="U2I75_000088"
     CDS             95078..95647
                     /gene="rnmV"
                     /locus_tag="U2I75_000088"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN1695255.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            95640..96524
                     /gene="rsmA"
                     /locus_tag="U2I75_000089"
     CDS             95640..96524
                     /gene="rsmA"
                     /locus_tag="U2I75_000089"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN1695256.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            96975..97604
                     /locus_tag="U2I75_000090"
     CDS             96975..97604
                     /locus_tag="U2I75_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695257.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(97728..98837)
                     /gene="ugpC"
                     /locus_tag="U2I75_000091"
     CDS             complement(97728..98837)
                     /gene="ugpC"
                     /locus_tag="U2I75_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN1695258.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            99046..99468
                     /gene="mgsA"
                     /locus_tag="U2I75_000092"
     CDS             99046..99468
                     /gene="mgsA"
                     /locus_tag="U2I75_000092"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN1695259.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            99791..100327
                     /locus_tag="U2I75_000093"
     CDS             99791..100327
                     /locus_tag="U2I75_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1695260.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(100367..102130)
                     /locus_tag="U2I75_000094"
     CDS             complement(100367..102130)
                     /locus_tag="U2I75_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695261.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(102133..103848)
                     /locus_tag="U2I75_000095"
     CDS             complement(102133..103848)
                     /locus_tag="U2I75_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695262.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(104627..104851)
                     /locus_tag="U2I75_000096"
     CDS             complement(104627..104851)
                     /locus_tag="U2I75_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695263.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            105042..107819
                     /locus_tag="U2I75_000097"
     CDS             105042..107819
                     /locus_tag="U2I75_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1695264.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            107900..108319
                     /locus_tag="U2I75_000098"
     CDS             107900..108319
                     /locus_tag="U2I75_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN1695265.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            108331..108801
                     /locus_tag="U2I75_000099"
     CDS             108331..108801
                     /locus_tag="U2I75_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1695266.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            108818..109597
                     /locus_tag="U2I75_000100"
     CDS             108818..109597
                     /locus_tag="U2I75_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1695267.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            109584..110405
                     /locus_tag="U2I75_000101"
     CDS             109584..110405
                     /locus_tag="U2I75_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1695268.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            110422..111471
                     /locus_tag="U2I75_000102"
     CDS             110422..111471
                     /locus_tag="U2I75_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1695269.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            111484..112488
                     /locus_tag="U2I75_000103"
     CDS             111484..112488
                     /locus_tag="U2I75_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1695270.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            113121..114140
                     /locus_tag="U2I75_000104"
     CDS             113121..114140
                     /locus_tag="U2I75_000104"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1695271.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        37900 a        19803 c        23441 g        33019 t
ORIGIN      
        1 attcactaat aataaagata tatttacagt atcattataa tgtattcaat ttaaaataag
       61 agactttggt aataatgaaa gaggaataaa acttttcttt ggcaaaacaa actccttgct
      121 atgagacgcg tttcatacac tatactcaaa gtatcaaaag gagggcttgc aatgaatact
      181 tatgtagaga taacagatgg cacaacgaac aattattttt atgcgtttgt cgagttcaag
      241 ggatccgtat taacgttgta cagttttcaa gggttgaata ggaatatagt aaaagaaata
      301 ccaatgaatg aaatcgaaaa tttgacaaaa gatatatatt ggggcgggca gcggatcagt
      361 ttttcgcatg aaggaaaaat gtatcagttt tttgaatgcg gtccagctgt ggtagactat
      421 ttacaagaaa atctttttgt ataggtggcg aagcagcagt ggcaaacatt cgagatatcg
      481 caaagctcac aggttattca gtttctacta tttcacgtgt aatcaatgga catccatatg
      541 tagatgagga aaaaaggaaa gaaatacttg cggtcatgaa agaagtcgat tatatcccta
      601 atgcaaatgc cagacagtta agttatggaa aaacgaaaag cattggagtg attctgcctt
      661 ataccaatca tccgtatttt gatcagttga tcagtgggat cattgaagca gcgtttgatt
      721 atgattacaa agtgacactt ttaccgacgg gctatcataa agaaaaagag cgaaggtatc
      781 ttgaagaatt tgcagctaaa gctttcgatg gattgatcat tacgtctcgt gccaatacac
      841 tagatgattt attggcatat caaaaatatg gacctctagt tttttgtgaa gaaatcaagg
      901 gaaaacaggc aagctgtgtc tttattgatc gagaacaatc aattacagaa ggtcttactt
      961 atttgaagca gcaaggtata aagcaattgg gcatcacgtt ggggcgaagt ggcaggctaa
     1021 gttataactc aaaaatcact ttgcagctat gtcatcaact ttttccatct tttgatgaat
     1081 cacttgtttt ttgggattgt atagatgcag agaaaggaaa acaagcaggc ttctttttta
     1141 aagagcttgg agttgatggt gtctttacaa attcggatat ggttgcagca ggcatcttgc
     1201 aaagctatct gcaagataag acaccagtgg tgattggacg cgataatttg ctgatcagcg
     1261 agcttcttgg tttcccaacc atcgaccacc atctggaagc ttgcggagaa atggcatttc
     1321 aattgtttct tacagaaaaa aaggataaag tgaaaatacc ttatacgttt attcaacgat
     1381 gaatatattt aaagtaggat gaaaaaactt gtgaaagaat ggagcagaca ccaaaagtta
     1441 gcataaaaat ctaacttggg tgtctgctgt tttgttatta ggtcatttta cgtgtacttt
     1501 tttgttggat catgtaaaag attacgcctt tttttggata atttcattta aagcttttgc
     1561 tgcaccatga ccacttcgct cgtcataata tgcagtgaac cgttcatcgg cttcgtacat
     1621 cattcctagt cctttatgcg cttttggctg atattcaggc cagctgtaag agaaccactt
     1681 cttatgagct tggaagatac ggtctgctaa gctttcatct tctggttctt ttaagtaaag
     1741 agtgagatca aataacatct gcttttcaac agtttgcatt tcttcatacg tttctttatc
     1801 catattcatc catttttcat tggatgctgt aacctttttt tttccgtatt tttgtcgaat
     1861 ctcttgtcca taggattctt cattttcttt caacttttgt tgtttaaacc cttcaaattt
     1921 ttctttgtca ctcatttttc ttcctccttc gtacatatca agtgtctgtt ggacagttgt
     1981 caagagggta tcgatttgtg cccgtttttc cagcaacatt tgctgatgct tgattaaagc
     2041 tttctggtgg tcaaaagatg gattgtctaa gacctcttgg atatcatcca acttgaatgc
     2101 cattgttcga taaaataaga tgtgttgcag acgatcgact tcttttttgc cataaatccg
     2161 ataacctgaa gaattgatcc tagctggttt tagcagtcca atctcatcat aaaagcgcaa
     2221 ggttctcgga ctgactcctg atatctctga cattttcttt attgtgtact ccattatttt
     2281 tcctcctgac aaatgtaagc ataatccttt acgtaacgag atggtcaaga aaaaattgta
     2341 tttttttctc acgaaattat gttatactat taagtgataa aaggtaaatc cttttgtctt
     2401 taaaggttca attgtaaccg attccttaat gctaggaaag atggtaacat aacttaagat
     2461 tggatctaaa tcgactatat agaaaagagg aatgagatga agctaatcga aacacctgtg
     2521 aatagtaatt taaatattaa gacattttat cctaaagtag tggaattctt ttttgggaat
     2581 acggctatta attattacaa attattttcg ttagatcgaa cacaactgct attggtagac
     2641 acatatgata aaaagcaagt cgtgatgatc aatacgaaga aaaaaatcac aaggcaagaa
     2701 atcgactatg cgattcatca cgttttaaaa atgacacgag aagatgtcaa agtccatgta
     2761 ggagtgaaac aggagctaga acgtgccgga atacaattca aacgtccaaa taaagatatt
     2821 gtagtgatag aacaaaaaaa tacgaaggat tctatttaat aacgactaat aatcatcttt
     2881 atggtaacct gctccatatc taatgtatac aataaaaaac aaggcgagac tgattgatgt
     2941 ctcgccattt ttgtttccat tctattgaca gctgctttct tactttgtat ctttttaaaa
     3001 acgtgtgata ttttttccgc gagttttttc aacgcaaggt atatcttgtc ataattcgcc
     3061 tttccaaaaa aattcaatac ataataagtt atatccgttt ctttggggat aagaagcaaa
     3121 aaaggaaaga cgtattattg cttcaaaaac ttttgccatt gattatgttg tagagacatt
     3181 tggttatatt tgttcaatca tcggtatctt aacggctacg attgtagtag gaaaatctac
     3241 ttattttatc aagtaagaaa aatagaaaac gctaatttct tcgtgtctcg gatacattga
     3301 ataaaataag tattctttct cgtttaggtt aaataccaat aagaaaggat agttttagca
     3361 gatggagttc atttttagca agaaatatcc gtaaaacatg tgctttttca gaaaaaatgg
     3421 aagatttctt ttcctattaa ttttttttgt tataatgggt aggaaatgaa aacttgccaa
     3481 gaggagtaat acaagatgaa taatacttac ccagacgaca gtttgacgct gcatacggat
     3541 ctatatcaaa tcaatatgat gcagacatac tgggaattag gtcgagctga tttacatgct
     3601 gtttttgaat gttactttcg agaaatgcct tttaaccacg gatacgcggt ttttgcagga
     3661 ttggaacggt tagtcaatta tttagagaat ctgacgttca atgattccga tattgcttac
     3721 ttgcgtggat tagaagtcta tcctgaaggt tttttggagt atttacaaaa ttttgaattt
     3781 aaagcaacag ttcgttctgc tcgtgaagga gaactagttt ttgcaaatga accattaatc
     3841 caagtggaag ggcctttagc gcactgccaa ttagtagaaa cagcactgtt gaatatggtc
     3901 aatttccaaa cgttgatcgc gacaaaagca gctcgtatca aatctgtcat cggagaagat
     3961 ccactattgg aatttggaac tagacgggca caagaactgg atgcagcagt ctggggaact
     4021 agagcggcat atatcggcgg agcagatgca acaagcaatg tacgtgctgg gaagatattt
     4081 gggataccag ctagcgggac ccatgctcat tcgttagtcc aatcttatgg aaatgattat
     4141 gaagcattta tggcctacgc taaaacccat aaagactgcg tgttcttagt cgacacttat
     4201 gatacgttga aatcaggtgt gccaagtgcg attcgcgtgg cgaaagaatt aggtgacaaa
     4261 atcaacttcc aaggcgtacg catcgacagt ggagatatgg cctatatatc taagcgtgtt
     4321 cgggaacaat tagatgcagc cggatttacg gaagcaaaaa tctatgcttc gaatgacttg
     4381 gatgaagcaa cgattttgaa tttaaagatg caaaaagcta aaattgatgt atggggtgtt
     4441 gggacaaaat tgatcacagc ctatgatcag ccagcccttg gtgcagtatt caagctagta
     4501 tcgatcgaag atgatgaagg aaaaatgatg gatacgatca agttatcaag caatgcagaa
     4561 aaagtaacta ctccagggaa aaaacaagtg tggcgcatca ctcgaaattt tgatgggaaa
     4621 tctgaaggag actacgtgac attatgggat gaagaccctc gagaagaaga agctatcttc
     4681 atgttccatc cagtgcatac gtttatcaat aaaactgttc gagactttac agctcgccca
     4741 gttttacaag atatctttat cgaaggaaaa agagtttacg aattacctgc actaaacgaa
     4801 atcaaagaat atacaaaaga aaatctagat tcactatggg aagagtataa acgagacttg
     4861 aatccgcaaa aatacccagt cgatctgtct actgaatgct ggaaccataa aatggctatc
     4921 atggaacgaa tgaaaaaaag cgtagcagaa cttcatacag aagcatagga ggagaataaa
     4981 tgagcctgca gacagaaatt atcaatgaat taggtgtcaa accaacaatt gatccaaaag
     5041 aagaaatacg taaaagtatt gactttatga aagcatattt gaaaaaatat ccgtttctta
     5101 aaacttttgt actaggtatc agcggcggcc aagattctac tttagcaggc cgtttggcgc
     5161 aactaacgat ggaagaaatg cgtgaagaaa caaaagatga aaactatcaa tttatcgctg
     5221 ttcgtcttcc ttatggtgaa caagcagatg aagaagatgc aaaagctgct cttgacttta
     5281 tccagcccga tgttagtctg cgagtaaata tcaaaccagc tgttgatgca caagtacaag
     5341 tattaagcga agcaggtgtg ggaatcagcg attttaacaa aggaaatatc aaagcacgtc
     5401 aacgtatgat tacgcaatat gccgtagctg gagaaagagc aggagccgta ttagggacag
     5461 atcatgctgc agaaaacatt actggattct tcacgaaatt tggtgatggt ggagccgaca
     5521 ttttgccatt attccgtttg gacaaacgac aagggaaaca attgcttcaa gcattgaatg
     5581 caccggaaaa attatacaca aaaattccga ctgcagatct tgaagatggg aaaccaatga
     5641 ttgcagatga agtcgcacta ggtgtcacat ataatgaaat tgatgattat ctagaaggaa
     5701 aagaagttcc cgctcaagca caagaaaaaa tcgaagcatg gtggaacaag acacaacata
     5761 aacgacattt gccaatttcc gttttggatg atttctggaa ataacaataa agcaaaaaca
     5821 gccgtccctt catagggatt ggctgttttt gttttataaa atagaaaaga aaaatatcag
     5881 ctgtagtaaa cttttaattt gatatcctga tcgtaattac cgaatccttt accaaataaa
     5941 gtacagcccc ccacatgttt ggcatcgtct ttcacttcga ttcgcaaatg ccaagtgtct
     6001 tgctctttag ggatgtcgtt gatcgtgatg tttgacaagg gttctccatt catattcgtc
     6061 aagtgtttgg taattcggat cgtcttcagc aagccgtatt gattaagatt atctggatac
     6121 caatcaggcg tatatttacc gcggatatca gcaaaatctc cagggctcgt ccatgttcct
     6181 agctccacac catttaggct gaaagtgata tctgaaggcc aattgtcatt tgaaaaagga
     6241 aattcagaag aaatttccac agaaacttcc aacatctcca atgtatcctc tgtatttaaa
     6301 aaattaggtg cctgatactc gacaaatccg ctggtaaacc agagaatgcg ggcatccatc
     6361 cttcgaggat ccataaaata gcgtggttca tccactttcc cgataaaatc atggatcgta
     6421 gctaaaccgc atgttggttc aattgcataa tttgtatagt gaccaatagg tatcgatgtt
     6481 tctttcgtat caaaagcatt gaatattttt tctggaaaac tgatatcgat tttatcgaca
     6541 cgaaggctcg aaattttttg ttgtcctact ttttctgttt tgatgatgtt tgcttcctct
     6601 agctttttca cgtgcatcgt cgtgatagca ctgctcactc caagttcttt tgccaaatct
     6661 ttgacattca tctttttttt ggaaagtagc tggatgatct tgatccttgt tttactagct
     6721 aatgcttcat ataccggtaa ggattcttcg tttatttgta gttgcatata tatcacctct
     6781 taaaaggttt ttcttatata ttaacatatt tgttaataaa agttatatta aattatttgt
     6841 tttaatcatt attaatattt tggttaatta aaatcgataa aaaataagta agtttaacat
     6901 ttatattatt gacaacgctt tcaacgaggt gtattattca tgtataaatt aaaggaggtc
     6961 agttatggaa gccacaatgt tgattagtag tgtcaagcgt gtaggcaaag ttgatccgcg
     7021 gttgtacagt tcatttattg aacatatggg acgagcagta tatgaaggga tttatcagcc
     7081 agatcatcca tcagctaata aaagtggatt tcgccaagac gtcattcact tggtaaaaga
     7141 attgaacatt cccatgatta gatatcctgg tggaaatttt gtgtcaggat ttcgttggga
     7201 agacagtatt gggcccatag caaatagacc tagaaggttg gatttagctt ggcggacaat
     7261 tgaaacgaat gaagttggga ttcatgaatt ttataactgg tgtcagcatg tgggggccga
     7321 aatcaatatg gctgtcaatc tgggtactcg tggagtagat gcagctcgta atttagtcga
     7381 atattgtaat tttcctaaag gaacttattg gagtgattta aggcgttcga atggtcaaga
     7441 gagccctttt ggaattaaga catggtgttt agggaatgaa atggacggtc cttggcagat
     7501 tggacataaa acagcggaag aatatggacg gttagcagag gaaacggcta aggcgatgaa
     7561 actcgtagat gattcaattg aggtggtttt atgcggaagt tcgaatcggc aaatgcctac
     7621 atttggtgat tgggagttaa cagttcttga tcaagcgtac gatcaaatag attatttgtc
     7681 tctgcatcaa tattatggca accaaaaaaa tgatctttct cattatcttg cacgttcctt
     7741 agacatggat cggttcattt ctgaagtgac agcgatgtgc gatacagtca aggcgaagaa
     7801 acatagtaag aagcagatca acctgtcctt tgatgaatgg aatatttggt atcattcagc
     7861 ggaacaagat gagcaagcag cgccttggca gattgctcct cctttactag aagataacta
     7921 taattttgaa gatgcattac tattaggctg tctattaatc actttattga aacatgcaga
     7981 tagagtaaag attgcttgtc ttgctcaatt ggtaaatgtt attgcaccaa tcaggacaga
     8041 aacaaacgga gaagcttgga gacagacgat ctattatcca tttatgcaag ctgctgtgta
     8101 tggacaagga gaggtgttaa gcccacaaat tcgttcagca aactatgcga cggaagattt
     8161 tgaagaagtg ccgtatttag aaagtatagc cgtaataaat gacaaagaac ttgtcatatt
     8221 tgcagttaac cgtggagaag aagaaatgaa tctaactgtt cacttgtctg aattatcatt
     8281 agagggagtg attgattttt ctgaaatgag tggatttgat acaaaagcaa ctaatcttcc
     8341 taaaagtgaa caagtaaaac caagtcattc aacaaatgta aaagctgaag atgggaaaat
     8401 caattgttta ttgaaaccgc tatcttggaa tgtggtcaga tgtcgtttat cagatgaagg
     8461 aggaacaaaa aatgaaaggt aaaaaatggt taattggctc tttgttggta gctgcaacag
     8521 gtattttagg agcttgcggt ggcggaagtt cagcagaatc tgattctgat aaagaagtga
     8581 ctttgacttt ttggaatggg tttactgcat ctgatggtga aatattgcaa gaaatcgtta
     8641 atgaatttaa taaaacaaat gataaaaaca tcaaaattga aatggatatc atgacttggg
     8701 ccaatttgaa tgaaaaactt cctacagcga tttcttctaa aaaagcacct gactttattg
     8761 cattaaatta tccagatttt gcacagtatg tagaaaacgg agcggtacaa ccattagacg
     8821 atttctggga atatgatgga gtagataaat ctgattttaa agaaacggct attgaattag
     8881 gtgaagtaga aggtaaacag tattttgtcc ccatgcaggt acaaggcatg tatatgtatt
     8941 ggaataatga tttgtttaaa caagctggat tagatactga acaaccacct actacttggg
     9001 aagaattgac agaaatggca ccaaaattaa ctgatagtag taaaaatgta tcaggttttg
     9061 tcttcaataa ggatggaact gcaccgttat ataactggat gttagcaaat ggcggtaaat
     9121 tagtgaacga tgattatact aagtctgaat ttgcgtctcc tgagaacctt aaaacgttaa
     9181 aagcgatcca aaaaatgatc catgtagaca agactggtcc agaatcgatt tctggcgccg
     9241 aaatggacaa tttaatgaat gctggtcaac tagccattga aatcaatggg ccatggttaa
     9301 ataacggatt gaaagcaaac gagattgatt ataatgtgac taccttacca caagggagtg
     9361 attcagacaa aaaaacagct attttagatg gcgtaggata tgccattccg gcaagtacag
     9421 ataataataa aaaagaagct gtctatgagt ttttgaaata ttggaataca actgaaattg
     9481 gtaaaaaatg gagtatcgaa aatggttttc ctgcctactt ggattcagta gcaaaagatg
     9541 aagaagtaaa aaatgatcct attgtatctg aattatcgaa acaaatggac tatgcagagc
     9601 catatttacc aggttttacc caaatctctg cgatcaataa cgatatcatc aatccactga
     9661 ttgaaaaatt attagcagga gaagatcctg agacactcat gaacgatgca gatgatgcca
     9721 tcaatcaatt actgcaaaaa tagtacagaa ctttatacag gatagactgt tggcaagata
     9781 cagatatctg aatgatcgac ctagtatttg cagataggtg ttattgcaaa aatcacagcg
     9841 attgttcgga tatcttattc agattcgttt aaaccaagat tgtatatttt atcaaaatga
     9901 aagatgaggt ggactatatg aacggattca ccaaatattg gaatcgtcct gatcgtgctg
     9961 cgtatttgtt tttaactcca tcttttttag ttttattatt atttactgtc ataccgcttt
    10021 taggaacatt tgctattagt tttacagatt tgaatatttt ctttacatca agaaattttg
    10081 taagttttga aaatttcaca cgtatattta gtgatgaacg agcagttaat tcgttgattc
    10141 attctttata tttcaccgta ctcgaaacgc caacacaaat tatcgttggt ttattagcag
    10201 cagtagcatt atctaaaaat accagattca atcgtttttg tcgttcgaca ttttacattc
    10261 cagttatttg ttcattgacg tcaattgcga ttattttttc tatgttgctt gatccgaatg
    10321 taggggcgat tccttacttg ttttcacaag tcggcttgcc gatccctcaa ttttttcgcg
    10381 atccaaccct tgctatgccg acggtagcca tcatgactgt ttggaagaat tttggtgtta
    10441 ctatgacgat tctcttgact gctattcagg gaatctctcc ctctttgtat gaatctgctc
    10501 aaatggatgg tgcaacaaat cgacagcagt tttttaacat tacgctacct cagatcgttc
    10561 cctccttagg tttttgtatt ttaacgaatc tgattggttc gatgcaagtg tttgaccaag
    10621 tatatgttgc aaccgctggt ggcccacaat ttaagacaga aacagctgtt caatatattt
    10681 acagtcgagg atttacagca ccttatgaat tgggctatgc atcagcactg tcttcggttt
    10741 tgtttatcat tgttgcaact atagctatct ccactaatgt gtacatgtca cggaaagaaa
    10801 aacaaatgaa ataggaggga aacctgtatg gcaccagaaa gctcgattac agttactgtg
    10861 aatagtcgaa aagcagttag tggtaaaaaa atcattcgaa cgattagtat gctagttata
    10921 ttgttggtta ttgcgtttcc ttttttgtgg ctgattattt cttcttttaa acacgaaaaa
    10981 gacattatct catttccgcc tagaatattt gcagatagct ataccctaga taactatatt
    11041 aaggtttgga caacgatccc tttattagat tacatcaaaa atacagtgat ttttgctgga
    11101 gggacagtga tcacttctgt attctttgat tctcttgcgg ggtatgcatt tgctcgtatg
    11161 cgtttcaaag gaaaatcagt tttgttctat ttcgttttgc tgacaatgat gatcccattc
    11221 caagtcttca tgattcctct atttattgaa gtcaatttgc ttggcatgct tgatacttat
    11281 gcaggtttga tcattcctcg tatgacaact gcatttggta tcttcatgat gcgttccttt
    11341 tttattactt taccagattc tttggaagag gcagcacgca ttgatggatt aagtgagttt
    11401 aatatttttt tgaaaatcat gttgccattg agcaaaccta ctcttttatc attagggatt
    11461 ttcacgctga tgaatagttg gaatgacttg ctttatccat tgatattgac aagtagttca
    11521 aaaatgagaa ctttaccagc agggttagct ttatttacag ggcagaacat ttctttttat
    11581 ggacctgtaa tggctggaac ggttatttca atgctgccat tactagtggt ttatattttt
    11641 gcacagaaat attttgttca aggaacagct atgtctggaa tgaaagagtg aggaagatga
    11701 aaatagttga gactaaactt tatggacagt tgattgtggt ttctgattac atcttattag
    11761 gtattctctg ggtaatcgtg tgtttgcctg ttattactat cgtgcctgct acatgtgctg
    11821 ttttttatgt aatggcgcaa tggaaaggtg agggatcagg aaatatcatt tattttttct
    11881 ttcaaggaat aaagcaacat tttggtaaga acttgttcct tagtctgtta acgatcatta
    11941 tttattttct tgttaatcaa ttattagaag aacagacaat cgtactttca attagtggat
    12001 atgtagtttt acttatttat tccatgtttc ttttatcctg gatatatgtg atttctgaaa
    12061 acaaagatat cacattttta caaatttttg aacaaagtgc atggaaagta ttttttgatt
    12121 ttttgagaaa tattctttgt tgcgtattgt tattaggatt tgtactgctc atatttcttt
    12181 tccctccttt tatttttatc tttgctggtg gcgtatggaa acttgtatct attatcttga
    12241 ctataggaaa tgaaaggtag ggaaagctgt tggacgaaga agtgaaagta aaaatcagag
    12301 aggattttac ggtaggagag atcagttctc ggctgtttgg ctcatttgtt gagcatatgg
    12361 gaagtgtcgt ctatacaggg atttatgaac cgggccatga aacagctgat ggtgacgggt
    12421 ttagacaaga tgtcctctct tatgtaaagg aattagaatt aggtgtcatt cgctatcctg
    12481 gaggaaactt tacatctggt tatgattggg tggatactgt aggtaggaaa gaacaacgac
    12541 caaaaaaaat tgatttagca tggcgaggta tagaaccaaa ttcttttgga ctccacgaat
    12601 tttttaaatg gatcagtcat gtacaagcga aaccgatatt cacggtgaat ttaggaacaa
    12661 aaggaattga tgaagcaaaa aatttagtag aatattgtaa ttttccaaaa ggaaccttct
    12721 ggagtgattt aagaataaaa aatgggcaaa aaaagccttt tggcgttaaa ttatggtgtt
    12781 tagggaatga gctagacggt ccatggcaag tgggggctaa gagtgcttat gaatatggta
    12841 ggttagcaaa tgaagcctca aaagtcatga agttagtgga tgattcaatt gaaacaatct
    12901 tagttggaag ctctacgcca cgtttaccat cgtaccctga atgggatcgt atcgcattag
    12961 aagaagctta tgagaatgta gattatttag ctttgcataa ctatattgat aagtatgatg
    13021 aagaagactt gactaaacct cctaaaaatg agcgtgatga tacagcgaca taccttgcaa
    13081 aagcttatcg attcgatcgt caaattgaag aaatcgttgc tacttgtgat tatgtaaaag
    13141 gatatttgag aagtgagaaa acaatgtatc tagcgtttga tgaatggaat gtacatgccc
    13201 aaccggaaaa aagacatagg gattttgaag tcggtagtcc catcgattgg tgttacttta
    13261 ctatggaaga tacattatta tttggttcat tagggctggc tattttacga cacgcagatc
    13321 gcgtaaagat tagttgtcaa tcattgttag taaacacaat acctttaata ctgacagatg
    13381 ctaaaggaca agcatggagg aatccaactt attatgtgat gcaacactta tcgaaatatg
    13441 ctaaaggtca agtattgaaa caacaaatca tttgccctgt ttatgattgt gaaaagataa
    13501 aaaatgttcc ttatgtggac agtgtgatcg tagataacgg caatcagctg actattttct
    13561 tgatcaatcg cacaaatgaa caacaaaaga taacgattga acatgatttc aatttgctag
    13621 atcaaggaga gcactacaca ttaaccagta gcgatctgtt ggacaaaaat acacgtgaag
    13681 aaccagaaaa aataaaacct gaaataaact atttttcaat agaaaaacat gacgaattaa
    13741 atgtagggat agaaaaatac tcttggaatg taattagaat aaagaaaatg tgaggaaatt
    13801 tgttatgatg aagacacttt ataataatcc tatcgttcgg caacgggcag atccttgggt
    13861 atataaacat acagatggtt attattattt tacaggatcg gttccaggct accaagtgat
    13921 tgaattaaga agggcaaagt cactgaatga attagaacat gctgcaagcc tgattgtatg
    13981 gcaggctcat gaagaaggac cgatgagtga attgatctgg gcacctgaag tccattatat
    14041 caatggaaaa tggtatattt actttgctgc aagcaatcat aaaactatac gtgatgaatc
    14101 tcatcatcac cgcatgttcg tacttgaaaa tcagcatata gatccaatga atacagattg
    14161 gatagaaaag ggacaaataa aaacgaaaag ggaaactttc agtttagacg gaacaacttt
    14221 ctcttctcaa ggaaagctat attatgtgtg ggcccaacaa gatcctgaaa ttactgggaa
    14281 ttctaatctt tatctatccg aaatggataa tccttggacg ttaaagggga agcagatcct
    14341 cctttctata cctgagtatg aatgggaaaa acggggtttt tcagtaaatg aaggtccagc
    14401 agtccttact cgcaatggga aaatattcat tacgtattct ggtagtgcaa cagatgaaaa
    14461 ctatgccatg ggcctgctgt gggctgatga gcacaagaat cttttattaa aagaatcatg
    14521 gcataaatta tcggagccag tattcgtttc ttctgaaaaa aataagcaat ttggtcctgg
    14581 tcacaactcg tttacggttt cagaagacgg gtgctttgat actttgatct atcatgctcg
    14641 accagagaag aaccaaaaag gggatccgtt agacaatcct aatcggcacg cgaacgcgca
    14701 atgctttacg tggaatgagc aaggatttcc agaatttggt gaacctgttc cgtatgcctt
    14761 atgagcacaa gaaaccttga tttttgcttg aataaacctt gcagactcgt tatgatatat
    14821 gtacgtacaa ataaaaaggg gcaaggcaaa tggcaaaata tcaagatatt gcagaagaga
    14881 taaaacaaaa gatccttcgt gaggaatacg aagtaaatac aaccattccc tcagaattca
    14941 agctgcaaga aatatatgac gtcagcagac atacgattcg gcaagcaatc gctgtgttgg
    15001 taaatgaggg ctatctgcgt aaagaaaaag ggtcgggcac atatgtagat gacagctata
    15061 agaaagaaaa aacaccattg aacagaacaa aaacgatcgg tgtcatcacc acatatctct
    15121 ctgattatat ttttccgtcg attatccgag gaatcgaaaa tgaattacga gaatctgggt
    15181 attcattgct tttagccagt acgaataacg atcacgaatt ggagaaagag tgtttgaaga
    15241 aaatgattgc ttatggggta gatggtttga tcgttgagcc tacgaaaagt aatcagtata
    15301 acccaaactt agcactttat gttgctttga gagaacaagg cattccgatg gtgatgatca
    15361 atgctgctta tgaagagtta aatgcctctt atatttgttt agatgatgtg aaagtcggtt
    15421 ttatggcagc agaatatttg ataaaaaaag gacatcagcg tctgttgttc atcacaaaaa
    15481 tcgacgatct tcaaggaaaa tacaggatga aaggttttat cagcgcgtgt gaacagtata
    15541 atgtgcgttt tttgacggaa gatatcatta ctttcaccac ggaaacacga gaaacaatcg
    15601 ctgaaaaaac agtacaacgg ttgaaagaag aacaagcttt agatggcatc atttgctata
    15661 acgatcaaat agctagttta atgctagata agttgacaga aaatggctat catattccag
    15721 atgacctatc gatcgtagga acagacgatt cttctttaag ccatttaggg aataaaaaat
    15781 tgacaacatt gacgcatcca aaagaaaagt tgggacaaga tgccgcacgg tggatcgtca
    15841 caacgattga aaaaggacag gcacagccaa atattctcta tgaacctgta ctagttgaac
    15901 gggaaacagt gaaagaaaac gaaaaataag agacgggttt ttaccgcctc ttattgctcc
    15961 tacggttact cgtcgcaatg tttgtgagag cttttgtcat cgctcaaggc tgaacgtttc
    16021 tttcaccacc tctccactaa acggtgttct cctaccaact ggtcacaagt cacaatattc
    16081 atcaaacatg agaagctgtt ttttgaaatt gttccttgtg actggaaact aagcggttcc
    16141 ttcttatttc ttgaagctga ccacttctgt cacaacctct ttaagaatga tggatagaaa
    16201 tacgtttagg ataagttttt ttagatgcac tttgttcgat ttcctctaaa gatttcccac
    16261 gagtttctgg tacgatctct tgaataaata aaacccctaa aaagcagatg atcccaaaaa
    16321 tgccgaaaac gatttcttga gacatagaag cagtcatcac aggaaaaagc aagccaacta
    16381 ggaaagaacc aatccaatta aaagaagagg ctaggccaga tgctcgcccg cggacagcta
    16441 gtgggaatat ttcgcctacg attacccatg tcagtggagc ccatgtgcaa gaataaaatg
    16501 cgacataaat acataaaaac aagagaatca acaacgaatt tgtatgagca tccagtacag
    16561 ttcctaaaac agctggtagg atgaaagaaa gtcccatcac gctaccgcct attttcaata
    16621 aagttcgccg attgaacttg tctgcaataa ccaaaaagag caaagaacca gcaactaaaa
    16681 tgattccttg gatgatcggc cacatcaatg catcgctcgc tgcatgccca gtcgcttttt
    16741 ctacgatcag cggaatatag taaaaaatag cattagctcc ttgaaattgc tggaatactg
    16801 ctacaccaat accggcaatg actaaggaac gatatttttc taaaaacaaa gtatgccaag
    16861 aagttccggt tgtcttttct tgtttgacat tcagctggat ttgttcgaat tcctgttcag
    16921 cttcttttgg tgtccgaata tagttcaata ctctttttgc ctcttccaag cggccagatt
    16981 gcataagaaa acgcggtgac tctggtagag ctaaaacacc gaaaaataaa atcagcgcgg
    17041 ggacagcagc gagccctaac atcacgcgcc aagccatcgt ttcaggcaac ccttttagca
    17101 aataatctgc aacataggaa aggagcatgc cagaagcaat catcacttga ttgatccctg
    17161 acaatcttcc ccttagtcta gcaggagcca tttcagacat ataagcagga actagggcag
    17221 atgcagcgcc aacagctaaa cctagaagaa tccttgctgc aatcagaaaa taagaaccat
    17281 tgtgaggggc agatgcagaa aggacagaac cagccataaa aataagtgca gaaagaagaa
    17341 tcatttttcg tcgtccaagc ttatcagaaa gaagaccaga taaagagccg ccaaggatcg
    17401 cacctaacat gaccgaagac gtgatccatc ctatcaaaga agctttacct gccaaacccc
    17461 aatcatgctg taaaaaaggt aaggcgccgg tcataacacc aatatcatat ccaaatagaa
    17521 tgccgccaaa tgcgccaaaa aaataaataa acgatgagga aacttttttt tcttttttca
    17581 taagtattct ctccttctag aatttcgaga aggcggttga tcagattttg taagcaattt
    17641 cattttataa cttatgtacg tacaaaacaa gtgtttttta ttgtattctt gtaaataact
    17701 gcttgacatg tacgtacaaa agtatacaat aatcattgta agcgctacta aaaagacggt
    17761 ttgatcaggt ttgacgagaa ggagggagtt aaatcattat gaaggaagta aatgcgcaaa
    17821 gtatgttaga tgtcatagat ggtcgtacat ctttagggat tgagtttgga tcaactagga
    17881 tcaaagctgt attgattgat tcatctttta ctcctattgc cgctggtagt tatgagtggg
    17941 aaaacaaact agaaaatggt atctggacct attcattaga agatatttgg aaaggactgc
    18001 gcgtcagtta ccgaaaaatg gcagcagaag tattcgaaat ctatggcgag aatcttacga
    18061 caatcgggtc gattgggttc agtgcaatga tgcacggtta tctagcattt gatagcaagg
    18121 aacagttact agtaccattt cgtacttggc gaaatacaat cactgaagaa gcagaacgaa
    18181 tattaacgga aaaatttcaa tacaatattc cgcagagatg gagtatcgct catttgtatc
    18241 aatctatttt gaataaagaa gaacatgtga aagaaattaa ttttttgaca acgcttgcag
    18301 gttatgtcca ttggcagctt actggtgaga aagtactagg cgtaggagat gcatcaggca
    18361 tgtttcctat cgatccttca acgaaaacct accatcaaga aatgaaaaat atttttggtc
    18421 agttaatcga aaaagcgcac gtagcgattg acttggatca cattttgcct aaagttctag
    18481 tagcaggaga agaagctggc aagttaagcg aatcaggggc ttatctgatc gaccctacgg
    18541 gaaatttgag accaggaatc ccggtttgtc cgccagaagg ggatgcagga accggtatgg
    18601 tcgctacgaa cagtgtagcc aaagggacag ggaacatttc ggctggtact tccgccttcg
    18661 ctatgatcgt attagaaaaa gaactagaaa atgtccatcc ggaaatcgac atggtcacta
    18721 caccttcagg agaactagta ggcatggttc atacgaataa ctgctcttcg gatatcaatg
    18781 cttgggtcag tatttttgaa gaatttacca attctttggg tatggagata agcagagata
    18841 aattattcac ggtattattc aacaaagcgc tagaaggcga tacggattgc gggggattac
    18901 tttcttatgg ttatttttcc ggtgaaaaca ttacaggagt aaatgaagga aggccattat
    18961 ttgtacgtac accaggtagc cgttttgatt tagcgaattt catgcggatc catttagcca
    19021 gtgcttttgg tgcaatgaga atcggaatgg atatattgaa atctgagaat gtccaaattg
    19081 atcgtttagt ggggcatggg gggattttta aaacaccaga agttggacag cggattctag
    19141 catctgctat ggaggcacca gttacagtga tggacacagc cggagaaggt ggagcgtggg
    19201 gaatcgcgtt attagcagcc tatatgatgg atcgacatgg gaagtcttta gaaaacttct
    19261 tggcagaaga cgtgtttgga caagaccaag gaatcaccat cacaccttca gcagaagaaa
    19321 tagcaggcta tcagatattt atgagacgtt accgtgaggg gatcgacatt gaaaaaacag
    19381 cgattcatca attgcaagaa aaaggagtga tagaaaatgc tggaacaact gaaagaagaa
    19441 gtataccaag caaatcttga cttgcctaaa cacggactcg tcaaatatac atggggaaat
    19501 gtgagcgctt ttgatcctga aacacgatat ttcgttatca aacctagcgg agtcagttat
    19561 gaagagatga cagcagacga catggtcgtt gtggacttgg acaatcatat cattgaaggt
    19621 aaactcaatc cctcttcgga tacgcctact catgctgttt tatatcgttc atttcctcaa
    19681 atcggaggaa tcgtccatac tcactcgaca tgggcgacga tctgggcaca agcaggattg
    19741 gatgttcctg cgatggggac gacgcatgca gatacttttt atggatcggt accttgtgct
    19801 cgatttttga cacaacagga aatcgacagc gggtacgaat atgaaacagg aaaagttatc
    19861 atagaaacat tcaaagaacg caaaattgat cccttagcaa ttccaggggt acttttacac
    19921 ggacacggtc catttacttg ggggaaagat gcgcaaagtg cggtaatgaa cgcagtcgta
    19981 ctagatgaag tatgcaagat gaatttattt accagacagc tcaattcatt ttcagaagag
    20041 ctgcctcaac ggattttaga taaacattat ttacgaaaac acggaaaaaa tgcgtattac
    20101 ggacaaaaca aataaaagga gtggctaatt atgttgaata ttggagaaaa agaattttgg
    20161 tttgttgtag gatcacagca tttatatggg gaagaagcac tgcgagaagt aaagaaacat
    20221 gcacaagaaa tggtcgatga actgaatgaa aatggccaat tgccttatcc aatccgcctt
    20281 caagaattag cagttactgc tgatacgatc acaaaaatca tgaaagaggt caattatcga
    20341 gaagaagtag caggtgtgat cacttggatg catactttct caccagcaaa aatgtggatc
    20401 cgcgggacaa agcttctaca aaaaccattg cttcatttag ctacgcaata taacgaaagc
    20461 attccttgga aaacgatcga tatggatttc atgaatttaa atcaatccgc tcatggtgac
    20521 cgcgaatatg ggttcatcaa tgctcgatta aataaacaaa ataaaatcgt cgttggctac
    20581 tggaaacgac ctgagatcca aaaagagatt gctgattgga tggatgttgc agtagcctat
    20641 aatgaaagtt ttggaataaa agtggctcgt tttggtgaca acatgagaaa cgtgggtgtc
    20701 acagaaggag acaaagtgga agcacagatt caatttggtt ggactgttga ttattttggt
    20761 atcggagatc ttgtacaagt aatcgatcgc gtatcagacg aagaagtcga tcaattattt
    20821 gaagagtaca aggaattgta taccttcgac tatggtgatt atgaagaaaa gacatgggag
    20881 gaacatgtaa aagtacaagc tcagcaggaa atcggtattc gccgtttctt agaagaagga
    20941 ggctataacg cttttacgac taattttgaa gacctgtatg gaatgaaaca actacctgga
    21001 ctagctgtac aacgtttgat ggcagaagga tacggttttg ctggggaagg cgactggaaa
    21061 actgcagcga ttgatcggtt gctgaaaatc atggctcgag ggaaagatac aggattcatg
    21121 gaagattata cttacgagct agctagtggc caagaagcaa tcttggaatc ccatatgatg
    21181 gaagtagatc cgacgcttgc tgcgacgaaa ccaagaatcg ttgtttctcc tttatcaatg
    21241 ggagatagag aagatccggc gcgtcttgta tttgatggga aagcaggaga aggggtagtc
    21301 gtatcgatgg cagatttcgg tacgcattat aaactgctga tcaatgaagt agaagccttt
    21361 gaaccaacga ctgaagcacc taatttacct gttgcacgtg tgttatggaa aactaaaccg
    21421 aatttccatg aaggtgtcca ttcatggatt caggcaggtg gcggtcatca taccgttgtc
    21481 tcgctaaatc taacaacaga tcaaattgaa acgtgggcaa aattagttga gttggaaaca
    21541 gtggttatta gataaaaaga ggttgtgaaa aagccactta gttttaagaa ataagaagag
    21601 attgacgaaa tttgcttttc aacttttttg aaatttagtc ttattatcaa tgtcagctaa
    21661 cggaacacca tttatcaaga tatctcaccc tcaatatttt gctctcgaaa ataaggctta
    21721 tggcagtaac ttgtcatagc cttattttta tcactcagcc tactctgctc taactgctct
    21781 ttttgcttct aggaagtcaa atggtaaaca gttgtctaat tgcagaaatt tcgagatact
    21841 tgttagtaag gagggggttt tatgtatcac gttcatttga ttgatgctat tccagaacaa
    21901 gagatagaaa agttacaagg ggaatatttc aaaataatag atgatttgcc agccgaagga
    21961 atagttattc gtagtacgaa agtgaaagat gaatggctta caccagattt gctagcaatt
    22021 tcaagagctg gtgttggggt gaacacgatc aacgttgaaa aagcttctga aaacgggacg
    22081 atcgtcatga atacaccagg tgtaaatgct aatgcagtaa aagagcttgt tttgtgctgt
    22141 ttgctcttat cttcccgtcc gataatcgaa gcaagtcgta tggtacaaac actaacaggt
    22201 cctaatattt tagaacaggc agaaaacaaa agaagtgctt atgtgggacg agaattgcaa
    22261 ggcaaaacca tcggtcttct tggtcttgga gcgataggca caaaagtagc gctaagttgc
    22321 tatagcttag ggatggatgt cttgggttat tctatccgcg atgcccagtt ggattatgtt
    22381 cggcaagcag atttagaaac agttctttct acctcggatt acatcgtagt catgctgccg
    22441 ttgacagaag atacaaaagg attgatcgat caggcgaata tagaaaaaat gaaaaaagat
    22501 gctgtactta tcaatgtagg aagaagtgaa attgttgata aatatgctgt tatgcaagca
    22561 ttggaaaaac aacatctggc taaatacttg actgattttc cagaagaaga gttcttagaa
    22621 aatgaccgta tacttatgtt gcctcatcta ggggatcgac tcaggaagct ttggcagaca
    22681 gcggacgttt ggcagttgaa gccttaaaag attatctttt gttcggtacg gttagagaag
    22741 cggtcaatta tccatccgca cgaatgtttt tccaagcgcc gtttcgattc actatatttt
    22801 atcaaaagaa aaccaatata ttagcagaga tattttctct gctgaatgag aacgagctag
    22861 cgatcgcaga tatatcgcgt aaccataaga atgagcatgt ctacatattg attgacatag
    22921 acagtactga ttttgaacag ctcaatcaag tgaaacaaca gctagaaaaa atttctggag
    22981 tacgaaaggt ccgcttatta aagaaaccag aaaggccata aagaaagaga atatttggat
    23041 ttcattaatt cttctttccg ttctttactt tcgaatttcg tacaactaaa ataagagaag
    23101 caacattctt ttattgatag aaatgaagga gcaagcattt gaaaagatat ataagcagac
    23161 tttcgaccaa acagaaaaag attttaaaag gaacagcagc aatcatcagt atcttttttc
    23221 ttgttgtttt tagtaattta tttttgcagt ggtgtcaaaa tgatctatcc gtagaccttg
    23281 cgttgaaatt tgctttttct tggcatacgg aaaaattctt cttagcctgt ctcgtcttgc
    23341 tgattattct tatttttctg attgcactag caggatctgt ccctttaggt tcccttacgt
    23401 atgtcgtagc catcggtgta ttaggttttg caaattatat gaaaatgagt tatcggcaag
    23461 aaccgattta tccagacgat ttgaagatga tcactgaaat cggattgctg aaagatatga
    23521 ccggcactat gctgtttaca gtgatcttag ctgccgcagg aaccgtgata ggattgtttt
    23581 gctggtacat gtttcgaagc ctgaaaaaag gaaggcgatt ccaattgatc cgattaacga
    23641 cactgcttgt ggcaattgga ctgctagggt atatcagtaa tttcaacaat cccgataatt
    23701 tgttgcgaaa agcatataat aaaacggcac tatggatacc ttacagccag aaaatgaact
    23761 actataacac tggttttatc ggtgggtttt tgtacaatct gaaggtagaa ccaatggatg
    23821 aacctgaagg atattcgaaa gaaaagatca aagaaatcac tgaaaaatac cagaagctag
    23881 ccgatgaaaa aaataaaact gtagaggaag agtcgccgaa catcgtgttc gtgatgagtg
    23941 aaagtttctc agatcctagt cgattaaacg gcgtagaagt atctggtgag ccattggcag
    24001 attattatga agtagctgat caaacatata gtggcaatat gctttctcaa aactatggcg
    24061 gtggaacagc aaatatcgaa tttgaagcat tgactggttt ttctatggcg ttatttaatg
    24121 cgcaactcac caccccttac acgatgcttg taccaaaaat ggatcagctt ccttctatcg
    24181 tttcagcgtt aaacgcacaa tcataccaga cgacagcgat ccatccatac aatacttcga
    24241 tgtataaaag agaggatgtt tatcagacat taggatttga ccagttcatc agcgagcgta
    24301 cgatgaccta tacggatacg atagagaata atccatatat ttctgatgag tcagcttaca
    24361 aagaagtaat gacacttctc aaggaagaaa aaacacctca gttcattcat ttagtcacga
    24421 tgcagacaca tatgccctac gatggaaagt atgataatct agactatcct gcaaaaactt
    24481 cagatggaag tgggacactt gacttagaga attatctgca agatatttcc tatagcagca
    24541 cggcactgaa gcagttcacg gaagaattga agaatctttc tagaaggact ttagttgttt
    24601 tttgggggga tcatcttcca gggatctatt cagatacgat ccaagcaaaa aatgacaaac
    24661 aaacattgca tgaaacacaa tttcttatgt tcgacagtaa aggagaatta gaaaagcaaa
    24721 cgactcacga tgcaatcacc agtccgtttt attttgcagc gaatctaatg gaacagacga
    24781 atcagacaac aaatggcttt tatcagctgc ttttatcatt agaacaggaa cttccggcgt
    24841 ttgaacgaga actgtactat caaaacggac agtggtataa agaagcgcaa ttcaatcgct
    24901 cgcaacaaga gatttacgac gaatatcaat tgatccaata tgacattgtc gcagggaaac
    24961 aatatagttt agcagatggt ttctttgagc acgaataaga atggcgagct gtatagaaac
    25021 cattaaattt tttcaagtac ttgtcatttg aggataaaaa caagtagaat aaataataac
    25081 aacaatggag aaagtaggaa aacgaacatg ttaccgaata ttggctatta tgtgcagtcc
    25141 atcgatcaaa tcgtcaagga aacagaagca atcggagaag gaatgcatcc gaaatatgaa
    25201 gtgatcagaa acgcaattga tgaaggaaaa acaagtgaac tggacaaatc gactcttgaa
    25261 gagatcatcc tgttatttga agaaggaaca aaaaaatatc gggcaatgct tatgaaagtc
    25321 agtactttgc gtccaccagc aaaagcaatg ggcattcata aaaattttga aaaagcttat
    25381 ctttcatatg tagcaggctg tgaagaaatg atccaaagta tcgatcttga atcaggggta
    25441 gatgaagatc tattcaatac atctgaagca aaacaagacc aagcgacaga ccaattgaca
    25501 gctgccatcc aaaaaatgac taatttgttg ttgaaaaaat aaaagaaatc tttgataaca
    25561 ataaaaacta gagttatcta atataaatgt aatattacct ttatgataga gtgatcaaaa
    25621 tcctttatga taatgacata cctataacca acaacaacct tgtacttact caaaatcgag
    25681 taaatactcc ttttccgtcg agtctaacca tctcggcggt tttttatttg tagaaaagaa
    25741 catcaggata gacggaaaag tcttgtcctg atgttctttt cagatagtat ggaaagctga
    25801 ttagttttct acattctgta catattgttc aaagtagttc tgtaaatcgg ctttgagttt
    25861 ctgatattcc tcttcgctat actctttatc ttcgattttc ccatcaaaga aaggaatatt
    25921 tagttcttcc cgtagtctgt ctaatacgtc ttgctgattc atttcattct ccttttctta
    25981 ctaacgatca aaataaacac agacacattc tggtacatag acatcttttt tcagtaattc
    26041 cagtaatcct gtttcaggat gacggcgata taatgtcaga ttatcggtgt tctgattggc
    26101 acacacgaca aaattattgc ttggatctag tgcgaaatcg cggggaaaat ccccttctgt
    26161 tgagatgatt tgcttgtttt gaatgtggga accatcttca aaaactgcga atacagcaat
    26221 cgagttatgt cctcggttag aagcataaag gaacttgcca tcatttgaaa tgcggatagc
    26281 tgcccctcca ttttcaccgt cgaattcttc tggcaaggtc gatattttct gtaattcttc
    26341 aaaagcacct gtttctttat tataagaaag cacgcggact gtgctgtcta attcaccaaa
    26401 taaataagcg aattgtccat ttggatgaaa gacaagatgg cgagggcctg ttcctggctc
    26461 tgcagtgaag gtagttacaa gattcaattt gccgttttcg gacacatcat aagtatacac
    26521 acgatctgta ccaagatcac atacgactaa tcgttggtca ggagtcagat ccgtatagtg
    26581 gacatgcgca tgatcttggt ttttatgagg acctgtcgct tcttgatgga atattgaatc
    26641 tgctgctttc agttcgccat ctcctaatat atggtagaca tttacttcac ctttatgata
    26701 gttcgccccg tatagtaatt gacgtgcttc atcaaccgct acataacaca aaggagcgcc
    26761 agactctgtt accgcattca aaaaatgaaa atccttatcg tacgcaccgg caccacctaa
    26821 tccatccaca ctagtaactg tgtaaagatt gccagcttta ctttttgcta cataggtagg
    26881 gctattttct tcaacagcta atgaaaggtt ggctaattct tctttttctg tatcaaggtc
    26941 gatcgtatag attcctttac tttcgcgacg cgtataggtt cctaaaatta tcttttcgat
    27001 catatgagct gattcctcct aattgtaatg aaatagtttt cgtttttatt ctatcacata
    27061 attattccac acggctaaag aatgagaatg tgttatactg tttttgaaag caattaaagg
    27121 agagagtgca atgattcagg caaagattac agaaattggg acagaagcaa taaacaagga
    27181 agaacccatg ctgattttgt tcgataaaac agcaacagct gccttacgaa aatattcggt
    27241 gattcaagaa gtatcaaaag acgaaccttt ttctttgaaa gcaggcggaa caattgcttt
    27301 tgatgatcag gtatatacga tcgattatgt cggtccgatg gccaatggga atttaaagac
    27361 agtagctcac gtatcattga tttttgatgc ttgtccagaa gaaggacaaa ttgcaaatgg
    27421 aatctatctt catccgcata gactgcctgc tattggtttg ggaagccaaa ttttttataa
    27481 ctaacaggag gaattatgga aggaaaaaat aagaagaaca gaccggtatc gtggttttgg
    27541 cgctggtttt taaataatca agtagtcaca gccttacttg ttgtattgtt aatattgctg
    27601 atcgtctttt tatttacaaa agtttcatat ttgtttgcac caatctggca atttttagca
    27661 atcgtaggtc tgcctatcat tttagcagga atcttgtatt acttgatgaa tccggtagtt
    27721 gattactttg aaaagcgaaa agtaccaagg ctttacagta tcattgggct gtttgttttg
    27781 gttgttgcac taatcgcatg ggggagcgta gtgatcatcc ctaagattca ggaacaaaca
    27841 gtgagcttca tcagtaactt ccctcaatat gtagatacga ttgataataa attaaccgag
    27901 attttgcggg acccgctgtt caaccaattt cgcgaacagt tggaaacagc aggtgataag
    27961 ttcatgaatt cggcaggcga tatgatccaa gatatatcta agagtacagt tcagagtctt
    28021 ggaagttttg tcggcgcggt agcgactatt ttagtggctc tgctgacgat gccgtttatc
    28081 ttattttatc tattgaaaga cggtaaacaa ttagcacctt attttgtcaa atttttaccg
    28141 acacgcatgc aaaaaccgac gttgaatgtg ctgagtgaaa tgaatagtca agtttcttct
    28201 tatataagag gacaactaac tgtggctttt gctgtggcaa tcatgtttat gattggtttt
    28261 gcggtcattg gtttagagta tgctgtcacg ttggggatta ttgcgggttt tttaaacctg
    28321 atcccttact taggatcatt tttagcgatg attccggcta tctttttagc cattgtagca
    28381 ggtccattca tgatcatcaa agtactgatc gtttttgcag tagagcagac aatagaagga
    28441 cgcttgatct ctccattagt actagggaat cagttagcaa tccatccagt aacgatttta
    28501 ttagttctgc tgacttctgg gaaattgttc ggaatcatcg gtgtgatctt ggggattcct
    28561 gtttatgcag cagctaaagt agtgattact catgtttttg aatggtacac aacaatctct
    28621 tctctttatg aagaaaatcc gttgaaaaag cagaaagaat aacaaaaaga gacagatggg
    28681 aaaatatcca aggcacaaat gctgctttga agattttttc ctctgtctct tttgtatgca
    28741 tttatctcaa tttttaaaat gatttaccta aagcagcagc tttatctaat gcattttgca
    28801 tcaactcatc tgcacggtca gggtcataat cgattccttc gatgaatagc tgatctactt
    28861 gatcgatgcc tacaaagttt aaaatacctt ttacatattg agaggcaaag tcttgtccgt
    28921 tgtatacacc gccattagat tggatgtgca gtgctttttt acctgtcact aatccttctg
    28981 gcccgttttc tgtatagcgg aacgtttttc cagctacatt gacggtatcg aaccaagctt
    29041 ttaattttgt tgggatattt agattccata aggcgtttgc gataacgatt ttatcagcag
    29101 caaggaactg atcagtcaat tcattgaaac gagcaatttt ttgttgttga ctttctgata
    29161 aggaagtgaa ttctgcacca gcacgtaatg cttcccaacc tgataataat tcttcatcga
    29221 tttccggaat atcatcgcga taaagttcca aaacagtcac ttcatcatct ggatgatttt
    29281 ctttataact tgttaaaaaa gacgtcaatg ccttaactgt gcgcgattct tctgcagtta
    29341 ggggatgacc tttgactact aaaacagatg ccatatgctt accttctcct tttattgcta
    29401 tgattgttat aactatgact aacaaaatca gtttactcga aaaagaaaag gctatcaagt
    29461 catattgata aggttgaaga ataaataact aaaaaaagac agggtataaa agaggctgtg
    29521 acacgatttt ttgtcacagc ctcttttgaa tctaacaagc aatattcaag caacactcga
    29581 ctgaagagct tccatcccac caatgatata aacagcacga aaaccattag cagtaagttg
    29641 atcggctaat gcttctgatc gcctgcctgt atagcttaga atataataaa gtttctttgg
    29701 atctagattt tttaattgtc ttggcaagat agcagcgggg atagaaagcg agtggcttaa
    29761 gtggtttttt gtgtaatcta aggtatttct tgtatctaaa acgatgctgt cagcatcgag
    29821 aggcaattta ttgaagtctt ggacactaat tgattgggcc atttatctca tcctttgttt
    29881 agtatgtggc tattttaatg ggcaactctc attaattcaa atggaaggat tcctcttatc
    29941 acttttttaa tgtatcggat gataatgaaa aaaactatgc aaatgtcttg caaaacaggt
    30001 aggaggtgtt ataatgacat agcgtcatat gacaatgtgt catcttgaaa gaaaggagaa
    30061 tccatatgcc gacacagaca tttttccatt tgcctaaaga aaaacaaaaa agactaatcg
    30121 aggctgcgag aatcgagttt tctagagtac ccttaaaaga agcttcgatt gctaatatcg
    30181 tcaaattagc ggatatccct agaggcagtt tctatcagta ttttgaagat aaagaggatc
    30241 tttattttta ttactttgag acaatgagac gtgacagctc taaagatatg ttacaactaa
    30301 tgaaagaatc cgatggtgat ctattcaaag gattcgaatc ctatttttct aaaatgattt
    30361 atgagatttt ggaaggcgag aatgcttctt tttatcgtaa cttgtttatg aacatggatt
    30421 accgtgcctc aagaaaagtc gctcctcata cgagtcgcca aaaaacgcct cacgatccac
    30481 ataaagaaca caaacaagat gcaaaagagt tgcttgaatt gatcgatcgg acgaagctga
    30541 aagtaaaaaa tgatcatgaa ttggaattac tgatccaatt agtgatgaat actgtattta
    30601 caacaattgc gcattcgtat cgcttatttt caacatctga agattactca gtagagcaag
    30661 caatcagtga attcaatttg aaactttctt ggctgaaaaa cggagtatat aaatagaaag
    30721 aagggaacta gatgctaaaa atcgtaaaac ggatctcctt gacttcagca attgcagctg
    30781 cagttttcat ggtgatccaa gtactggcag atttatattt gccgacactt acctccaata
    30841 ttattgataa aggggtagcg caaggagatg tcgattatat ttggcataca ggttttgtga
    30901 tgatcggctt ttctttgatc agtatcttag ctgctatcgc aaatacattt tttgctactc
    30961 gtgaatcaca gaaactgggt aagcaattgc gtacggatgt ctataaaaaa tcagagagtc
    31021 tgacaaaaga tgcgtttgat aaatatggaa cggcctcttt gatcacaaga acgaccaatg
    31081 atgtgactca aatccaaatg gttacacaaa tgtttttacg tatgatgatc aatgcaccaa
    31141 tcacgttgat tggcgcgagc atacttgctt atcaaaaaga tcatcagttg acaaaaatct
    31201 tcttagttgt catcccagta atgattattt tgatcggcgg aatcatgtac tttgctgttc
    31261 cattattcaa aagtatgcaa aagaaaactg accgtttaaa cctggtattt cgagaaggat
    31321 tgacaggggt cagagtgatt cgagcattcg ataagacacg ctttgaagaa aatcgttttg
    31381 atttagcgaa taaagattat acaaatacgg caatcaaagt taatacgatc gtcgcattga
    31441 tgatgccaat gatgacattg atcatgagcg gtacaaacgt agcaatcact tggtttggtg
    31501 gacactacat agcagatatg actttggaag tcggtaatct gattgcattt atgacttatg
    31561 cgatgcagat cttgatcagc tttatgatgc tttccgctat ttttatcatg gtgcctcgtg
    31621 cacaagcttc agctgaccgt attaatgaag tgttggatga aaaaatcggt atccatgacc
    31681 ctgaaaatcc taaaacagtt tcttttgctg gaaaaaacgc aacattggcc ttcaatcatg
    31741 tcaattatcg atatcatgga gcagaaaaac ttgcattgga agatattgat ttccaagcca
    31801 agtcgggcga gatcgtcgca atcatagggg ggactggttc aggtaaaacg accttagtca
    31861 atttgatccc tcgattatat gacatcgaat ctggttcgat ccaaataaat ggaacggaca
    31921 ttagtgacat gactcaatat aatttgcgag aattaatggg ctttgttcct caaaaagcag
    31981 ttctgttctc tggaacgatc cgagacaata tgcagtatgg taagccggat gcaacagatg
    32041 aaatgatttg gaaagcatta gaaatcgcac aagctaaaga ttttgtttca gagatggaag
    32101 atggcttaga tagccatgtg gaacaaggag gaggaaactt ctctggtgga caaagacagc
    32161 gtctggcaat tgcacgggca ttagtcaaaa cagcagatat ctatgtgttt gatgattcct
    32221 tctcagcgtt ggatttcaaa acggatgcca atcttcgcca agcattgaaa acgaatatga
    32281 aagaaagtat caccgtttta gttgctcaac gagtaagtac agtaatggat gcagatatga
    32341 tccttgtgct agatgaagga aaactggttg gcaaaggaac acatgaagaa ctattagcaa
    32401 caaacgaaac ttatcaagaa atcgttcatt cacaactaag agaggaggac ttagcatgag
    32461 taaaacaaac acacgtccct ctcatggacc agcaggcggt ccaggacatg gtggacctgg
    32521 cagaaatctc ggtgtaaaag gtgaaaagcc taaaaatttc tggggaacgg tacgccgatt
    32581 gtttggctat atgtctaaac gtatggttgc gatcatcgct gtatttgtac tagcaatagc
    32641 agccgtgatc ttccaaatcc aaacaccaaa ggtcttgggg aaagcaacca cagaaatatt
    32701 caaaggtgta atggctggcg ctcaacagat gcaacaaggc caacaagtaa caaaatttcc
    32761 gatagacttt gataaaatcg cgcagatcat tgcgacagta atcgtgatgt atttgatttc
    32821 tgctgtattc aactttttac agcaatttat tatgacacgt gtttcccaac gaacagttta
    32881 tgaattacgt catgatttgg aagccaaaat gaatcgtgta ccgatttcct attatgatac
    32941 acatacaaat ggggatatca tgtctcgagc aatcaatgat atggataaca tcgcaagcac
    33001 gctgcagcaa aatttgactc aatttgtaac tagtgcagtc acattgattg gggtcatcgt
    33061 gatgatgctt tcaatcagct ggcaattgac gttagtcgcg ttattgatga tccctttaag
    33121 cttgatcatc gtgatgatcg tagcgccgaa atcacaagtg tttttcgcag atcagcaaaa
    33181 aagtttaggt ttattgaata accaagtaga agaaacatat ggtggtcata ccattgttaa
    33241 aacattcaat catactgaaa aagaccagga agtatttgaa aaagaaaatc agaatctgta
    33301 tgccgctggt tggaaagctc aatttatttc ggcaatcatc atgccgttga tgaatttcgt
    33361 taaaaatcta ggctatgtgt tcgtcgctgt actaggcgga gtaaaagttg caaatgggaa
    33421 catgacatta ggggatgttc aagcgttctt gcaatataca acacagttct ctcaaccaat
    33481 cactcaactt gcaagtttga tgaacactat ccaatcaaca gttgcttcag ccgaacgggt
    33541 atttgaagtg ttggatgaag aagagatgtc caatgaaaaa tctggccttc cagaagaaaa
    33601 agatacacca tacaaagttc gttttgaaaa tgttcaattc ggctacacac cagataactt
    33661 gctaatgact gattttaact tggatgtcaa acctggtgag atggtcgcta ttgtaggtcc
    33721 gactggtgca gggaagacga cactgatcaa ccttcttgaa cggttctacg atgtttctgg
    33781 aggtagtatt cgttacgatg gtgtagatac ccgtgatctt tcacgtgatg aattgcgcgc
    33841 acagttttca atggtactgc aagatacttg gctgtttaca ggaagtatct atgacaatat
    33901 caaatatggg aacgacgatg caacagacga acaaatcatt gaggctgcta aagctgctca
    33961 cgtagacgat tttgttcgta aattgccaga aggatataac actgtattaa acgaggatgc
    34021 aagcaatatt tcgcaagggc agcgtcagct aatcacaatt gcgcgggcat tcttagcaaa
    34081 tccagatgtc ctgatccttg atgaagcaac atctagtgta gatacacgaa cagaaatact
    34141 gattcaaaaa gcgatgaacc gcctattgga aaaccggaca agtttcgttg ttgctcaccg
    34201 cttgtctaca atacgcgatg cagacaacat tattgtgatg aatcatggtt cgatcgtaga
    34261 aacaggaaac cacgatacat tgatggaaaa agacggattc tatgcagatc tttataacag
    34321 tcagttttct gaagaagaag cttcatagaa tagtacaaat ttattatttt ttttaggggc
    34381 tgtgacaaaa gtcgtgtcac agtccctttt tccgaataaa cggtgttcaa tcagctgtcc
    34441 ttcggtatta gctcaaaaaa cggcaaaata tgagaagcta tttttccgtt ttgtatctct
    34501 aatactcagg tcaaaccgct gattttacaa cctcttcctt aaacggtgtt ctcctgccaa
    34561 ctcctcggtc acaagtcaca atagtcaaca aacctgagaa gctgtttttt gaaattgctc
    34621 cttgtgactc ggagttaaac ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc
    34681 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt
    34741 ttcttactac tcgaaactaa gcgacttttt cacaacctct tccttaaacg gtgttcaaaa
    34801 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata ggaggagcta tttttgtttg
    34861 gctgttctta ttacttgatg cagaacagct ttttcacaac ctctttttat aagagttttt
    34921 actcattaat ttacaaaaaa caagcaaaat gcgaattttc tcacattttt tttaacaaaa
    34981 tcaaagttat tgcttgaaag aagaagaatc catcgttaaa ataaagcttg acgtagaggg
    35041 gagcaagaaa tatgaaagat tttcaattgg gggctgccac ctatttaaaa agtgtagcca
    35101 ttcgggtgaa agaccgagat aaaatgattg atttttacaa gaacgttgtt ggctttgatt
    35161 tgaaaagaga agaaaacgaa ttagccattt taggaacaga agaagctgga agtgaaatgt
    35221 tgctgctgga agaaagtcct agagcgaatg atcacatggg ggtgatcaaa aagctgaacc
    35281 gtttttcatt gattatccct agcacagaag aaatggcaga tatcctttgt cgtatccgta
    35341 aaatgaatta tccaatcaaa gaagctttag aagatcatgg acgtatgggg attttgctcg
    35401 tagacccaga agagaatgaa ttggaaattt accatgaaga gaataagaac gaccaaagag
    35461 aaattccaga aacaatggat caagaagcat tattaagaaa atctgaagaa aaattcagta
    35521 aactatcaac aggttcttat tttgaaaaaa tccatttaaa tgttactaac ttaacaaaag
    35581 aacgccaatt tcttaatcat gtacttggtt tgaaggtaca taatgaagaa gagggcatcc
    35641 atgtattgaa tgaaggagat ttccatgttg gtttgacaga agctagcgga ggcgcaattg
    35701 atttgcctac agatgaagtg attggacttg attttctgaa attcatgatc gatactgaaa
    35761 gcatggaagg actgattgct cacttgcagc aactatcagt tgatttcttc ttagacaaaa
    35821 agcacacagt actcacaatc tatgatgcta taggaatcga atggtggttc gtcagtgagc
    35881 caaaagaaaa gaataaaact gacattcgat gcgacgctta actttttctt tcttaacgat
    35941 tgtcgcatgt ctcgtcattt tatgaacggt catactatcg cttattctag agatagaaag
    36001 agaaaatagt ataccggggg aaactgcgcg gtttttcgcg cagttttttt gtgtaaaaaa
    36061 ggttgattcc tcatagatta agctttaagc ttggactgta agaatctgat agaaaagcag
    36121 gagaaaaaga tgtatccaca cacgaaacag cttattttga aaaagatgga agaaggattt
    36181 tatccaggtg tagcagtcgc cttcataaaa gaaaatcaag tagaaacatt tacagccggc
    36241 tttgcccaag tcgttcctga aaagcagctg atgacagaag atctgttgtt tgatgcagcc
    36301 tcactgacga aagtcgttgc aaccacttca ttagttttac gtctattaga agatggaaag
    36361 atagaactcg atcaaccgct tcataaatat cttcctgaat ttgaaaatca gacaatcact
    36421 ttgcgtcatt tactgacaca tacttctgat atcaaaacat ggataccaaa cagagataag
    36481 ctatctcagg aagaattgat cgccgcttat ttgaagctag aaccaggtga gcaactagga
    36541 aaagaagtaa aatatacaga tgccggtacg attcttcttg ggttgatgct agaggaaatt
    36601 taccaagaaa ctgcagtgga aatctttcaa gaacaagtat tggaaccatt aggaatgatg
    36661 aacagcttat ttcttccttt tccttccaaa aaaatcgttc ccacagaaca actgccttcg
    36721 ggtgaggttt tgcgtggaca aactcatgat ccgaaagcaa gagttttggc tgaacatgca
    36781 ggtaacgcgg gtctctttat tgatttgaaa gacagcatta cttttgtcca aatgtatttg
    36841 aacaagggaa aaatccgtaa tggtcgtttt ttagaagaag agacgattga ttctttactg
    36901 caagatcaaa caccgaaaaa aaatggctat cgttctttag gatgggactt aaaaaaggat
    36961 gtagaagacg gtcatgcgat tctttttcat actggttata caggtacgtt tttcttgatt
    37021 gatataaaaa cacaagaagc gttgatattt ttgtcaaatc gcgtacatcc tgtcgatcat
    37081 cgtgcggagt atctcatatg tcgagatgaa attgttgaaa actttttgaa agagaaagcg
    37141 gtattaccga aataatgcta taatagctat gaaaaatgag aggagtttga attatgcagg
    37201 aacctatgtt tttaaaacca gtttttcaag aaaaaatttg gggaggaagt cgtttacgtt
    37261 cagtctttgg ttttgatatc cctaacgata aaatcggaga agattgggcg atcagtgcgc
    37321 atcctcatgg tgtcagtgta gtagaaaatg gcgaattcaa agggaaaaga ttagatgaac
    37381 tatggtcaga acacaaggaa ttgtttggcc atccttcaga accagtgttt ccattattga
    37441 tcaagatctt agacgcagaa gatgatctat ctgtacaagt acatccagat gatgcctatg
    37501 ggatgaaaca tgaaggtgaa ttaggaaaaa ctgaatgctg gtatatcatc gatgctgaac
    37561 ctggtgcaga aatcatctat ggtcatcatg caaagacaag agaagaacta gctgagatga
    37621 tcaaagatgg gcgctgggat gaccttttga agaaagtacc agttaaaaaa ggtgatttct
    37681 tttatgtacc aagcggaacg atccatgcta ttggtaaagg aatcatgatt ttagaaacac
    37741 agcaaagttc tgacacgact taccgcgtgt atgactacga cagaaaagat gcgcaaggac
    37801 aaacaagaga gctgcatatc caacaatcga tcgatgtaac aactgtccct gcgaaaacac
    37861 cagaattaca aatcaaagaa gtccgaaaag ggaattcatc gatcgtcact tatttagaaa
    37921 ctgagttttt caatgtatat gagtgggata ttaaaggaat cacatctttc aaaaaacaag
    37981 ccccttatac attgatgacg gtaatcgaag gagcaggaga ccttgtcgtt gacggaaaaa
    38041 catatccatt agaaaaagga acaagctgta ttattccaaa tggcgtttct gagtgggcgg
    38101 tccaaggtga actagcaatc attgcgtctg aaccaggcaa atagttccca gatagctgaa
    38161 agcagcagac ggtttttcgt ttgctgcttt ttctacataa aatccgtcca tcaataaata
    38221 gaagtctggt gaaaaagatg gaatacaaat ggaaagcaaa caacagcagt cgcgtgtcta
    38281 tgaaacaact gagaaaagct ggacatcact taaatgaaat gatcatgcca aatctaagtc
    38341 tgtttatcgc ttggggaatc ttgtcattag caacaaaagg tgtgtctggc gatttgcaca
    38401 ccacattaga agaggtatgc cattggatga tccagctgct tttacccatc ttgatcagtt
    38461 attcaggcgg gaaatcggta gggggacaaa aaggggcagt cgccggtgca attgcttctt
    38521 tagggttgat cgctcgttcc aatgctccac aaatcgtagg agcgatgatt atcgggccgc
    38581 ttgccggcat cgtatatgaa caatttgttt ctagattcca tgtgaaattc aaggctggat
    38641 atgaaatgct tgtcagcaac ctactagtag gactgactgg tagcgttatt tgtataattg
    38701 gaatcgtttt tattgaacca gttttaaata gtgttcactt gttgctcgcc tccgttgtgt
    38761 cttggctagt cacaatgaat ctgttaccac ttgtcagtct gatcttagaa cctctaaaag
    38821 tactattttt caataatgca atcaatcatg gtgtattgac acctttagga atcgaattct
    38881 cacaaacagt cgggtactct tccttatttt tgatggaggc aaatcctgga cctggtttag
    38941 ggatcttact ttctatcctt tgttttgcag aaaaacagga aaaagtaaat gcgagtggag
    39001 cattgatgat ccatgcgatt ggcgggattc acgaaatcta ttttccattt gtcttgctgc
    39061 gaccgtatct atttttagct gtaatggctg gtggggcaag cggcacgttg atatttcaat
    39121 ggttagatac gggattagct gcacctgtat cgcctgggtc actactcgtg atttttgcca
    39181 atgcgcctta tccacaacta gctggggtaa gtatagggat agcaatcagt acatttgtga
    39241 ccttcatttg tgcagcgata ctattgaaat taccaaataa agaaaaagag aacaaaacgg
    39301 tcaaagaaag gggagaaatc ttggaaccat ctatcattca ttccgtcatt tttgcttgcg
    39361 actcaggtat gggatcaagt gctatgggag ccgccctgct gaaaaagaaa ttagcagcag
    39421 aaaaaatcga tctttctgtc ggatacagct ctatctatga attgaataac caagtaaatc
    39481 agctggttat cgtgcaacat gaactagcgg gtcttgcaag agaaaaagta cctcaaagtc
    39541 aaatctatgt actggatcac tttttggaaa cagatatctt gctgtcacga ataaaagaag
    39601 cgatccaatt tccaaagcgg gaagaaaaaa aagagatagc agatatcaca gaacaaaaaa
    39661 cgtcgcctgc taatattgta tttttgtata aaaacaacag acgcggctca caaactatgg
    39721 gcatgacgat tttaagtcaa ttagcaaaaa aagcgaaaaa acagtttgtt atctcaaaag
    39781 aatcaataga aaaaatcaaa atccaagaag aaacgattta tatcgcacaa agagaactag
    39841 ctgtaactga acagctagaa gaaaagatcc ctcggctagt tctagttgaa cattgggtag
    39901 ctacgaatga atacgaaaaa ctcgtgaagg gagaaatata agatgtattt atcgcccaga
    39961 gaaaaacaat tattagcaga atttctcagt agcccgtcac ctgtatctat ccaaaaaatg
    40021 atgaatttat tgaaggtcag taaacggact atttaccgcg aattagataa tcttgaagtc
    40081 tcattgaaat cggtaaatgc ttctttgaaa aaagcagcta gaggctcatt tgtcattgag
    40141 gcatcagaag aagtattaga gcaattaaaa gaaaatacag atgggaaaag taatgactta
    40201 tccactgcta aacgacaaca cgcgatttta gccgagttgt tactgacgac agagccgttg
    40261 agtcttgctc atttcatgga atcatatcgc atcagtaata ccacttttta tgcagatatc
    40321 aaacagttgg aagaaagtat tcgccaactg cctttggaaa tcattcggaa ccagggatac
    40381 gagatcgaag gaccggaaaa ataccgccgg cttctgacag cgaatgtatt agaattagaa
    40441 atcaatgaat atgaactttt tcatagtatt tctttcgatg catcactgaa ttattttttt
    40501 cgattcgttg atccgcaaca tctatctctt gcacgcaaag taatcgggga agaattgatc
    40561 caaaaaaaga ccaatctaag tgatagaaag cttgaacatt tggttttgat gctgacattg
    40621 acgatggagc gtgtgacgaa aaatcattta ctgacaaatg agacatataa tagtctggca
    40681 aacaaagaat tactacagac agctaaacga ttgttttcac ggatcgcttc tgagacaaaa
    40741 caactttatc cagtaaatga gatcgtcttt tttgcaggat tattaaatga cttcacgaac
    40801 tcatttgatc agaatttctt tgaagagacg ttcgatacac agcttgctta tttagtaaaa
    40861 caactgattc aagaagtaag tgaagagacg aatgtccatt tttatgaaga tgaaactttg
    40921 tacaaaatgc tgttgactca tttatctggt gtgttttcaa gagccgtttt acaagaagag
    40981 acactgacca atccgatttt agaaaaaatc atggttcaat acgaggaagt tgcgaatgca
    41041 atccgtaaag cagtagagcg gatttttcaa gaaaaaaaat tatccgaaga agagatcgct
    41101 tatatggtcc ttcattttgc gaattcattg gaacgaagtc cgaaagaaag caatatcagt
    41161 attgcaggat tttcgccaag tggattagct tctactagca tgttggaact acgattgaaa
    41221 aggtattttc ccttcatcca acaaattcat ttctatcgga ttgctgattt aaaaaaaatc
    41281 gatttacaag aagaatatga tgttgttatt tctacatcta ttttaccagg atacggtgga
    41341 aaatatattt tagtctcccc gctactgcta gaagatgaag tcaagcagct aaaagaagct
    41401 ttttcgcata ttgagaagaa aaaaaggcaa aaaattacgt ataacagcaa taacgagtgg
    41461 atgatagaag atacttatga ggaaacaatg catttcatgg atcaaatcaa tcaattactc
    41521 ggtcgttttt ttatccaaga actggataat cctgagacga tttccgaaac gattcgcagc
    41581 ttatttcctt attttcctga aggactagta acggatgaag aggtggttca tcgccggctg
    41641 cttaagcgat atgaacaagc acctatcggt attcctcata caaatatggg gttattccat
    41701 acatccagct cattgatttc taaaccgatc ttttgtattt tcaatttgaa acatccgttg
    41761 accattgaag ggatggatag acagccagtc gatttgtccc gtatgttagt tatgctggcg
    41821 ccttcgccaa tccaagaaaa agacgggatt cttttaggaa agatcagtgg agcaatcatc
    41881 atgaatgact tgaacaccga gatcttccgc tcaggaaata aagaaatcgt ttatcagttg
    41941 ttagcgaaaa tcctgattga agaaataaag aaatgaaggt gaaaagatga tacagctacc
    42001 agcagaaaaa atattgctcg atcaatccta tcaaaattgg aaagaagcgt tgaccgagat
    42061 cggccagtgg atgacccaac aagggggaat caaaccggca tatatccaat caatgatcga
    42121 aagacaagaa aaagcgagtg tctatatcgg gaatttcgta gccttgccac atgggactga
    42181 caaagatgaa gaagtagtag aagaaggaat ctatttagtc caagtgcctg atggtgtgga
    42241 ctttggaaca aaagaggaac caaaaatcgc gacactctta tttgtagtgg ctttaaaaaa
    42301 agaacatcag ttagcggtgc tccaagattt agcctttttc tgttcagaca tcgaccaagt
    42361 aatggcgctc agtgatgcgg aaaccattga agaagtccaa atgatttttg aacatggaca
    42421 tatataacca atgttcacag taaaaaaagc tgccttgcat caagtaataa cttcaaccaa
    42481 gataaaaata agaggagcta tttttgttgg gaggttctta ttactgcgga tgcaagcttt
    42541 tgaaaactat ttattctaga aaaccgttta tttgaaatag gggacggtga caaggttttt
    42601 gtcaccgccc ctattatttt tgtgttttaa tagctctttt ttatatagat agtgcccata
    42661 cctatgaata ataaaacgat actccatata ccaagtgtca agataccatt catgaaagta
    42721 acctgttctg aagctagtgg cgagaaaata tctcctccta tgatcacatt ttgaaaatca
    42781 acataagaag ttggcaagta atgactgatt gattctatcc atccaatcga agaaaacaag
    42841 cgatactctg ctaagaaaag acaaagaagt gtacctaaaa gattgactag agaatgttca
    42901 aataaagcag atagaagaaa actgattgta cttaaaaaga gcacccacaa caacaggaac
    42961 agaaagctct ggaaaagata ctcatacatc ggcaggattc gtatctcctg accaatgatc
    43021 gttcctacag gataacggaa gtctcccaaa ccatttttga ttattactag tatagagacg
    43081 atcaaaaatg gcatcacgag acttagcata gctgcaccat aataaatggt aaaacggatg
    43141 ctatgtttct ttaccatact atccggtaat agattgacta gagagattac ctgctttctt
    43201 ttttcataag agatgatgga agaaagaaac aaggcggtta tacataaaat caaaaaacta
    43261 gaaatcattc ctgaaaaaat attttcgtag taatttgcta aagagagatg cgctggcaag
    43321 tcaaaaatag aatatctttg gatctgatgt tctttcatat aacggagcaa ctcgacacgt
    43381 ttttgttgtt caatgattgg aattccttgt ctttgaccag aaatcagtcg atccagtata
    43441 tctttttcat aatgataagt tgcttcgacc atttgctgga cattccctgt gttttttgca
    43501 tgtaagattg cttcgatcag tttgttgctt ttctctaaac tttccacaaa ttctttctca
    43561 ttctcatctt ctttcaacgt ttcaatcaaa cgttgcatct cttcatagtt atgattcagt
    43621 tcatcgaatt ctgtataagc aagtttatga tcaaaaaaaa gtgggaatgc aaagagcacg
    43681 accaacaata gaggaaataa aaagtttact ttgtttttta gtaagagatt gcgttcaaat
    43741 gtaagtaaag acatagttac acctcatata atttacgata aatagctaaa atctcttcca
    43801 tatttgagga ttctgtgacg atttctccat tttttagaaa acatgcttta gaacaaatcg
    43861 attggatatt cgcgatagcg tgggaagaaa gaagaatcgt cttcccctct ttctgccatt
    43921 gtctgatgag gctattcaag atatcaatac ttcctgggtc taatccgttc aacggttcat
    43981 cgaataacat gacagatgaa tcactgatca agtaagcagc aagcaagaca tgttgtttca
    44041 ttcctagaga catttttcca atcggcaaag taaggtactc agtcatcttc aatcgttgga
    44101 aaacagtttg gcattctact ttgctctgcc aatagtattt gatcaaagtc aaataatcaa
    44161 cagccgatag atagtcggat aactgattcg ttgattctag aaaaaagaaa gaccggcgat
    44221 aatctgtata agcagtagga gagatgttgt gaagggacaa ttgatcataa gttgttggca
    44281 gcagttgggc aatgccattc agcaaagttg ttttgccaaa tccgttcggg gcaatcaaag
    44341 cagctacttc tcctgattct actaaaagat tcaattgtcg aatcacacgt tttttaccac
    44401 gtttcatgtt catgtttttg atcagtaatg tcatagatag gcctccaata taaaatttaa
    44461 taacataata gaatggaaaa catctaaatt aaatgctaac aaaagttatt gtttgcatat
    44521 tataaaaaaa ttaaaataac agttttattt aaaattgaag ttttctgata ccgaaatctt
    44581 tttattttgc tgacatactc aaacctttgg cacaaagttt cgtgccaaag tacgactatt
    44641 tctcttttta gtaagcggat acaattagag tatcaaaagg agagagggag agacaaaatg
    44701 gaacggacga ttcccaaaaa caacatttcg ctaaaagcga gggtacaaaa attaggcagt
    44761 actttatcaa gcatggtcat gccaaatatt ggagcactga ttgcctgggg tgtattaacg
    44821 gctctattta tcccagatgg ttatttacca aatgaggctt tcgctaccat ggtaagccca
    44881 atgctaactt atctgattcc tttattgatc ggttatactg gcggaaaagt aattgctggt
    44941 gaccgcggat cagttgttgg ggcaattgca acgatgggtg tgattgtcgg aacagatatt
    45001 ccaatgatgc taggcgcgat gatcatggga cctcttggtg gatatgccat caagaaattt
    45061 gatcagctat tccaaaaacg gatcaaatcc ggttttgaaa tgctagtcaa taatttttca
    45121 gcaggtctta tcggttttgg attagcgtta ttaggttttt ctgcgattgg tccagttgtc
    45181 gatgcattga ctcaagcaat ggcaaaaggt gtagagatca ttttatctgc ccatttgatt
    45241 ccattgacaa gtatttttat tgaaccagca aaaatcttat ttttgaataa tgcaatcaat
    45301 catgggattt tgacaccatt gggaactgaa caagtagcag aggctggaaa atctattcta
    45361 ttcttgcttg aagcaaatcc tggtccagga cttggcgtat tacttgcttt tactttgttt
    45421 ggcaaaggag cagcaaaatc ttcagcacca ggagccatga tcattcactt tttaggcggg
    45481 atccatgaaa tctattttcc ttacgttatg atgaaaccgt tattattctt atcagttata
    45541 ggcggaggaa tgtcaggaag tttcattttc caattgatgg atgctggact aagagcgcca
    45601 gcttctccag ggtcgatcat tgcgattcta gcgatgacac cgttcaattc ttaccttcct
    45661 gttattttgg gcgtgattgt tgccgcagct gtttcgtttg gtatttctgc agtaattttg
    45721 aaagcggatg cgaaagatac aaatgaagac ttcgaaaaat ctgttcaaga gacaaaggca
    45781 gctaagcaag cttccaaagg tgcccaaggt attcctcaag gagctacatt ctctggcgta
    45841 caaaaaatca tttttgcttg tgatgcagga atgggatcaa gtgcgatggg cgcttcgatc
    45901 ttgagaaaaa aagtacagga agcagacttg cctcaaacgg tgaccaatca agcaatcagc
    45961 caactgacag atgactcgaa tacattgatc gttactcagg tcgaactaca agaaagagca
    46021 aaacaaaaag caccgagcgc gcgttttgtc tcagtagaaa actttttgaa ttcacctcga
    46081 tatgatgaaa tcattcaaga attaagcagc aagcagaaaa catcagtttc tgaaattgag
    46141 aatgagccag cagaagagtt tctttcagca aacatcaaac aaatcaaaga gattctactt
    46201 gtccacgatg atcgttccgg atcagcaaca atggggatga ccgtgctcaa agatatatta
    46261 gacaagaacc atttaactat cccgttaaga aaagtcaata tccaagattt aaaagatgaa
    46321 cctgattcac tgatcgtagc caaagaaagt ttagcggaaa aagtacagat ggctgcacct
    46381 caatccattc atttatcagt caacagcatg gtcaatccgc aaaaatacga atcgattgca
    46441 caatatttga aacagacagc ttaagggagg aaaacagcat gcaaagtttg ccattcgaca
    46501 tgatcgagct caatcgatct tttaaaacca aagaagaagc gatccgctat tgcggtcaga
    46561 tgcttgtaga agcagggtgc gttgatgagg aatatatcga tgccatggta gatagagaca
    46621 aaatgttatc tgtttatatg gggaatttta tcgcgattcc tcatggaaca gatgaagcaa
    46681 aaaaatatgt gaaaaaatcc ggcatctgtg tcgttcaagt accagatggt gtagattttg
    46741 gtaccgaaga agaagaaaag attgcgactg ttttgttcgg tattgcagga gtcggagatg
    46801 agcatttgca acttgtacag caaatcgcac tttattgcag tgatatggac aatgttgtcc
    46861 agctagcaga cgcgctcagc aaagaagaag taacggaaaa tctagcaatt gcttaaggag
    46921 agagaaaaat gagagcagta cattttggtg caggaaatat cggtcgagga tttattggag
    46981 aggttttggc agctaatgga tttgagatta ctttcgtaga tgtgaatcac acgatcattg
    47041 atgctttaaa tgaaagaaaa gaatacgaaa tcgaattagc agatgaatca aaagaaaaaa
    47101 tcactgtaaa aaatgtcaaa ggaatcaaca atcaaacaaa tccagaaaaa gtaatccaag
    47161 aaatcgctga agcagattta gttactaccg ccattggccc gaatatctta ccttttattg
    47221 ctgagttgat tgcaaaaggt atccaagaaa gagaaaagga aggaagcaca acatctttag
    47281 atatcattgc ctgtgaaaat atgattggcg gttcagcatt tctagaaaaa gaagtgtata
    47341 aatatcttcc ggaaacatca tttactgaca aatatatcgg ctttccaaat gcagcagtcg
    47401 atcgaatcgt cccattacaa caccatgaag atcctttatt cgtccaagtg gaacctttca
    47461 aagaatgggt cattgacgat agccaaagaa aaaataaaaa aatccaatta aaaggggttt
    47521 tatatgtaga tgatctagaa ccatatatcg aacgcaaact atttagtgtc aatactggtc
    47581 atgcgactgt cgcttataca ggtgccttgt taggttacca gaccattgat gaagcaatgc
    47641 aagatgcgtt agttgtggca caattaaaat cagttttgca ggaaacagga agtttgctga
    47701 ttgccaaatg ggggtttgat gcagagcagc atcatgcgta tattgaaaaa atcattcatc
    47761 gcttccaaaa caaaaatatc tctgatgcga tcacacgtgt cgctcggaca ccattacgta
    47821 agttaggtta tcaagaaaga tttacacgtc cagtgagaga actacaagaa cataacttga
    47881 cttgcccaca tttgacggca acaatgggga ttatttttaa ttactatgat cctgaagatg
    47941 aacaaagccg tcaattgcat gaaatgaaga tccatgaaaa cttggaacaa ttgattcaag
    48001 aagtcacagg aatcaatgat ccgaaaacaa tcgggaatat caagcaaaac gtgaaccgtt
    48061 atgccaagca agtcgcttaa aataggatac gaaaaagggg aatgtgacga aatttttgtc
    48121 acattcccct ttttcgaata actaggctgt ttgtccttca agatagggca gatccaattc
    48181 tttaccgtaa cgttgttcta aagctaggcg gatcaaacga gctgccaggt tctcatttct
    48241 tgcaaggata ggtccatgga aataagaacc gaagacattt cggtaaatga caccttcaga
    48301 ttggtcttca ccattatttc cttgaccttg taccactttc cccaaaggtc gttcaccttc
    48361 acctaagaaa gtccgaccat tgtggttttc aaatccataa taagtttcat tgaattcttc
    48421 gttatggata acgatatcac cgataaaccg gttattttcc tgacttaacg tgtagtgatc
    48481 cagtgcactg attccttgga tcttttctcc attagctccc atgtagtagt gacctagcaa
    48541 ttggtagcca ccacaaatgg ctaacatgac gccatcattt tcgatatagt tgatcaaaga
    48601 atctttcttt ttctggatgt cttttgaaat gattaattgc tcaaagtctt gaccaccgcc
    48661 aaagaaaacc aagtcatatt tatcagcatc aaaaggttca tgtatactaa cgatctccgt
    48721 atggcaagaa acccccattt tttctgctat atatttcaac atcaaaaggt ttccgttatc
    48781 accatatgtg ttcatcaaat ttccataaag gtgggcgata ttgagttcat aattagccac
    48841 ggttcattcc tcctttgata aatccttgag cagctagttc tttacgaagc tgcaatacag
    48901 cagtgtaagt agctaaaata tagacatgct cagttggcaa ttctttgatt ttttcgatta
    48961 cttctggtaa ttcttttgtt tctgtcaatt tattttctgg aatcccggca acttttagac
    49021 gtaaagtcat atctgtatga cggtctcctc cagaaataac agctgggata tccatatcgg
    49081 caaagttttc aaaattacca tcccaaatcc agctgacatc gattccgtct gcgtaattgg
    49141 catttaataa agccactaaa gaaaatggat aaggagctag accaatcata tcgattactt
    49201 gattgatacc tacaggattt ttgacaagta ctaatgtaca ttttttccca ttgatattga
    49261 tgacttcttg tcggccgaat actttctcgt catatcctaa accttgtttg attttatcag
    49321 gatcgacatt gaagtactct gccactgcag tagctgctaa agcattgtag acattataca
    49381 taccgccaac ttcgattccg tattcatgac catcgatcac gaaatctgct gacacattgt
    49441 ccatccgtac catatcagtt aatcgataat ccaattcagg acgtttgaag tcacaatttg
    49501 gacagtaata tttccctaag tttgcataag tgatcatttt ataatgcaag atgtgatgac
    49561 aatttggaca taagacaccg tctgtattgt aatgagccat ttgttcacga tcttcttcat
    49621 ggtcaaagcc ataatatttt ctaggattga ctgtttcgat agaattgaag attggtgaat
    49681 caccgttact gataatcgtt gcatttggtg actttgcggc accgtctaca atcattttat
    49741 aggtcgtata gatttctcca taccgatcca tttgatcacg gaaaatattt gtaaataaga
    49801 acagttctgg tttcatatat tctgtgactt tgctcaaact tgcttcatct atttctagga
    49861 cagcaaactt tttctgtcct ttttttggtt ttgcctgcaa gaaagtcgat acaattccct
    49921 gtaccatatt cgcaccagta gggttggtta gaacttcatc gaattgctgg cgcaaaatat
    49981 tgactgttaa agcggtagtc aatgttttac cgtttgttcc ggtgacaacg acgacttggt
    50041 agtcttttgc caaactatct agtatatgtg gatcgacttt cagcgctagt ttacctggta
    50101 aacttgatcc gcctttaaaa aaagttttta atgcccattg cgacgttttt ccaactgcaa
    50161 tcgcaaaatg actgcgtatt cccattatgt aacctccaaa aacgatttgc tgacgaataa
    50221 attcaactaa tcataccata tgttgaaaaa aaggttaaat tgcttttctt attcttaaga
    50281 aaaggttacg gctgaaagtg catgaaatcg gacaaaatcg catgatttaa caaaaaaaca
    50341 gcttgaaaca aaatgattgt ttcaagctgc tgtgatttta ttattccctc ttacctatat
    50401 tcccatttta accgataata attttctcgc ttggatattc atagcctaga tctcgtgttt
    50461 cttttggcac gaataacatc aatgtatata gcattccgat ccgcccaata aacatcagtg
    50521 tagcaatcgt gattttgccc acaacagaca agtctccggt aatccctaaa gataaaccag
    50581 ttgttccaaa cgcagaagtt acttcgatga tgatcgagat cagcgtttgt tcttctgtag
    50641 cagataaaaa aacgatacaa aagaaacaca taccaagaga aagcataaag acaacgactg
    50701 attttcgcac atcatcttga tcgattctcc gtccaaaaat attgatgtta tcttcacttt
    50761 tgaggaagga atatagatac aaaccgatga ttgctacagt agttgttcga atcccgccgc
    50821 ctacagaact aggactgcat ccgataaaca tcagtaaaga aaaaatgatc agtgttgtga
    50881 tttgaaaatc acccaaatcg tgaatctgca gaccagcatt acgagtcgta atcgaataaa
    50941 acatcgaatt gatccatttg acactcatat tggaatcttg gaataaatgg tccttttcca
    51001 atagataaat caagacagtt cccgaaacaa acaagataac aaaagctaac acagctaatt
    51061 tggtaaacaa ggaaaaacga aacggaagct tcgcattgga acgtttgtat aatagccatt
    51121 cccggcactc catcaataca ggaaaaccaa ttcctccgac aaaaatcaaa aacatgatca
    51181 ggatcaagaa aaaatagtca tgtgcaaagg gcttgatgga atccccagtg acgtcaaacc
    51241 cagagttcgt gacagccgat atcgcctgat aaaaaccgta aaaaacggca tcacgccaac
    51301 gatcaaaata gcctctgtaa taaaaataaa tagaaaagaa tgtcccaaaa agtaattgaa
    51361 accagattaa aatagcaaaa gtgatccgaa tcagtcggac gataccagac aaacgcggtt
    51421 gattcatatc cgtcatgatc agctgtcgct gtttcagtgt gatccgtcgt ttagaaaaaa
    51481 tgacaaaagc agtagaaatc atcatgatcc ccaaaccacc aacttgaaat aatatttcca
    51541 gcagtataat accgttatca ttgaacacag aattgatatc aaaagtcgat agtccagtca
    51601 cactcactgt actgattgcc atgaacagca tgtccacaaa agaaacatgg ctatctggtt
    51661 cgcgaaaaat cggcatataa aatagaacga gtgataagac cgtcatcaat atataataaa
    51721 aaacgatgat ttggatcgat gagaaatgat tggcagcaaa cttctgtcct cgccgctgca
    51781 gccttcgaaa gaataaatca acatgcacag tactatcctc ctgacaatag tcgtttctac
    51841 cagtatacaa ggggaattag gaatactcaa agaaattatc aaacagataa caatacagct
    51901 ttcgattaaa atagaaatgg cttataataa ggaaaatcct agaaaaaaga aggaggagga
    51961 ataacaaatt gattaatgct caacaaatcg tagaaaaaat gaaaaatcaa aaaataaatt
    52021 atgatacagt tctgaaaaaa atgatcatgc aatgggagaa agaagaagta aagcctaaga
    52081 tattaataca tacatgctgt gctccttgca gtacgcacac attggaattc atgtctcaat
    52141 atgcagagat cacgttattt ttttcaaatt cgaatattca tcctaaaagt gagtatttgc
    52201 gaagattaca tgaacaaaaa cgatttgtag accaatttaa tgagaaaact gaccatcatg
    52261 ttgcgctaat cgttgatgaa taccgtccaa atgaattcac caaaatggtt ttaacaaacc
    52321 atcttgaaaa cgaaaaagaa ggtggtgctc gctgcagtgc atgcttcaat atgcgattag
    52381 atcttgttgc gcaaaaagcg caagaactag gatacgatta ctttggaagt gccctgacca
    52441 tctcgccaaa gaaaaatgca gctttgatca atcagatcgg aatggatatc caaaagattt
    52501 acgggacaca atacttgcca agtgatttca agaaaaacaa aggctatgaa cgctctttgc
    52561 aaatgtgcaa agagtatgat atttatcgcc aatgttattg tggttgcgtg ttcgctgcca
    52621 agcagcaagg atgcgatctg aaagaaatca atcgggaagc aaaagcttac ttaaaaacat
    52681 caaccgtagt ttttgaggaa aattaaagaa atctttcgga aaaacgtaag aaaaaaataa
    52741 agaatttatg gtagaaataa ggtagactgt ataatttgtc cgtagtaaag taaggaaaca
    52801 agaataaatt tcaatgaagg aagagataag atgcgaaaag tagcacctat tttgaagcca
    52861 atcgctgtat taacaatctc gacgattgtc gcgactacta caggtattgt gaatagttcc
    52921 agctaccgtg caccaagttc aagttctagt actgtagaag aaacaacacc aactacttca
    52981 gtctcgacat ctgaaccaac aacaacggaa agtttgaatg aaaccactac gacaagtaca
    53041 tgggacgata caacaactag ttcgtcgtat gatgagacat atacaactga cagctcaagc
    53101 gaaacaagta atacggtacc agatggcact ccatcggatt caaccaatcc aagtacaagt
    53161 ccggatacaa gtgctagctc cgaaacgata ccaagcagta cgactgtaga tgataacacg
    53221 caaaatgatg atgcagcacg tacagcacag caaaaccaag attcaggaaa tacccaaacg
    53281 tataaccagc aagcaggtga ttctcaatga catttttact agaccttatc aacggggtgc
    53341 ataaattcct tggctattta gacatcagtc ctaaatatct taaccgtgga tatacgattt
    53401 taagtgtgat tcccacgctt tatttattga gaattgttta tggactttgg caaaaccaaa
    53461 actatcttca atttttctta tatggacttg catttctagt gcttgtttat tttactgttt
    53521 taaatgtatt ttattacttt ttagataaaa acacgaaagc cgatgtcact cagctttttg
    53581 tgaagtattt acctgatgaa gcgttcaaca tccagacaga agaatcgata gataatggaa
    53641 attcgatcga taaagtgaat acacaagagg tcatggtttc ttatgacgaa gattatcaat
    53701 tgaaattagc cgagaatatg cggtatttga ttggaaatgg tgagatcaaa acaaatgaac
    53761 tcggtcgcat ggatggattt ctggtcgata gaaatacatt gtatccttat tactatgtca
    53821 agaaaacagg agagaaagag tatgttctaa atattggacg aagctactcg gacatggaaa
    53881 aagtagggac cattcgactt gactcaacag aagacacatt ggaacctgtc ggattgttca
    53941 tcgtcggcgg tgattttgtc aaagacgggt tacgctatca tgagccttat cgtctgaaat
    54001 tgattgcaaa aaaggcacag tcgcaggaag aaccagtggt ttatagtcgt agccaaagaa
    54061 gaagacattc ataatgtgat gaaacgccgt gtatcagtgg ttgcgataaa aacgtttatt
    54121 cggaataagg gggatgtgac acgtcttttg tcacgttcct tttttatata acgagaaaga
    54181 taagacgaac acatcactta ttctttcctt tcatttataa acattgtaga ataaagaaag
    54241 tacaacgaag tattgtgaaa aaacgtaata aataacgagg aaaatacaag taggaggtaa
    54301 gctgtgaaag aatttttgaa aaattggggg attttgatcc ttgtattagc ggctatttta
    54361 ttagctagag tgtacgtgtt tacaccagtt actgtaaacg ggcattcgat ggaccctaca
    54421 ttaagtgatg gacaacgatt gatttcatct aagatctcaa actatgaacg aatggatatt
    54481 attactacaa aggaaccggg ggatgaagaa cggatgattg ttaaacggat catcggtatg
    54541 cctggagata cagtcaagat ggaaaatgac cagttgacca ttaatggcaa aaagtatgat
    54601 gaaccttatc tggatgagtt caaaaaagaa ttttcagaag ataagttaca aggagaatat
    54661 gcttatagct caggattcca agcacaagca gaaagttcat ccacctttac gagtgacttt
    54721 gaatatactg tgccaaaagg aaagtatctt gttctaggcg ataaccgctt gatttcaaaa
    54781 gacagccgaa tgtttgggtt agtcgacaaa gatatgatcc aaggaaaagt tgttttccgt
    54841 tattggcctc tttcagaaat taaaatcatg tgatgaaaaa ataaaaagaa gctgtgacat
    54901 gttttttgtc acagcttctt tttccgaaaa ttccttctta tttcttggag ttgctcactt
    54961 tttttgcaat ctcattttaa gctttttggc tgatggataa gaaaagcgaa gcgtttttgt
    55021 tcataaatag cttttcccca cgatggaagg tatgactagt agcagagaca aagcccgttt
    55081 tttccaatcg attattcaat tcttcttggc taaagccatt atggactttc ggatgactaa
    55141 tttgttcatt cttgtcaaag tctacgatga tcaattgacc gccaggagct aaaatttcat
    55201 aaagttttgt aagaatattc tccgtatcag gaatgtgtaa aagtacaagt gatagaagga
    55261 tgagattagc ttggacagca ggaaattcga cagaagcatc agcgtggatc gttcggacat
    55321 ttttcagatc tgctgcttgg atcttttctt ctgccatttt cagcatagtt tctgaagcat
    55381 cggcaatgat cagttctttg aatctttccg caagcggtaa actaacaagc ccagttccgc
    55441 ctccgtaatc cagcaggact ttttggtggc tgtcgcgcgg tagataagta gttagttctt
    55501 cgcgaataat cttggctaat gcttgacggt caggggaatc ataatggtta gcgatggaat
    55561 caaaaatatt ttttgtcatc aaatagctcc ttacgtaaaa ctttgaaatt ttaacttatt
    55621 atagcacgtg aaaagaaaga aagcatgcta tactcaattc aaaaagggag aatctagaaa
    55681 gaatatgccg atatcgtttg taaaagatag agaggaaaaa ggaaagtgtg tccgagaaat
    55741 attactagac ttgcctgagt ggtttgggct tcctgaatca acggaaaaat atatcgagga
    55801 atcaagtaaa ctacctttat ggtgtgaaaa aagaaaagaa gaatatttag gctttatcac
    55861 tctttcacag acaagtgaag atactgccga aatctattct attgtatggg aataaaaaaa
    55921 gactatcatc accaaggatt aggaacaaaa ctttttaaag aagcagaagg ttacgcagca
    55981 aaacattaca aatatttgca agtgaagact gtagacgagg ggcactactc tatatatgat
    56041 caaactattt gtttctacga atcattagga ttttcaaggt tggaagtttt tccaaattta
    56101 tgggatgaat ggaacccatg tttagttctt gtcaaaaaat tggaacaaaa gtgagcaata
    56161 gatttaatac taatctgtgc ttgatgaaaa ttctcactgt aaaaaatgct gaaaatcaag
    56221 tataatacca taggaacaaa ctggaaggtg aaaaagagca tggcacaact attttttaag
    56281 tatggcgcaa tgaatagcgg caagacgatc gagatcctaa aagtcgcaca taattatgaa
    56341 gaacaaaata agccagttgt cttaatgaca agcggtattg atacaagaga tggtttaggc
    56401 gttgtttcaa gccgaatcgg actgaaacgt aatgcgatgc cgatatttga agaaacagac
    56461 gtatttgacg taattcagaa aatggaaagc aaaccctatt gtgtgctgat cgatgaagcg
    56521 caattcttaa aaaagaaaca tgtcattgct tttacgagaa ttgtggacga attggatatt
    56581 ccggttatgg catttggttt gaaaaatgat tttcgaaacg agttatttga aggatcaaaa
    56641 tatttattgc tttatgcaga caaaatcgaa gaaatgaaaa caatctgttg gttctgccac
    56701 aaaaaagcaa tcatgaattt acattatatt gatggaaacc ctgtttatga aggcgatcaa
    56761 gtgcaaattg gcggaaatga agcttattac cccgtttgtc gaaaacatta ctttcaccca
    56821 tcaatgatta cagaaggaga ataagcatgt acgatcaatt acaagcaatt gaagaccgtt
    56881 atgaagagct tggagaactt ttaagtgacc cagaagtgat ttctgataca aagcgtttta
    56941 tgcagctttc aaaagaagaa gcaaacacgc gcgaaacagt cgaagtttat cgtcgttata
    57001 aagaagtagt acaaggaatc accgatacag aagaactgct tggtgaaaaa ctggatgatg
    57061 aaatggctga actagcaaaa gaagaattat ctgaattaaa aaaagaaaaa gaagtcttag
    57121 aagaaaaaat caagatcttg cttttaccaa aagatccaaa tgacgataaa aatatcatca
    57181 tggaaatccg tggagcagca ggcggagacg aagcagcatt attcgctggc gatcttttca
    57241 atatgtacca aaagtatgct gaagcacaag gctggaaaac agaagtgatg gaagctaacg
    57301 ttaccggaat cggtggatat aaagaagtga tcatgatgat caccggagac aacgtatatt
    57361 cgaaattaaa atatgaaagt ggtgcgcacc gcgtccagcg tgttccttca actgaatcgc
    57421 aagggcgtat ccatacttcc accgcaacag ttgtcgtgat gccagaagct gaagaagtag
    57481 agatcgatat tgaagacaaa gatatccgta cagatatcta tcatgcttca ggtgctggcg
    57541 gacagcacgt caataaaaca gcatctgctg tacgtttgac tcacttacca acagggatcg
    57601 ttgttgctat gcaagatgaa cgttctcaaa tcaagaaccg tgaaaaagca atgaaaatct
    57661 tgcgtgctcg tgtgtacgat aagatccagc aagaagcaca aagcgagtat gatgccaatc
    57721 gaaaatcagc agtcggaact ggggatcgtt cagaacggat ccggacgtat aatttcccgc
    57781 aaaatcgtgt gactgatcat cgtatcggat taactatcca aaaattagat cagattttag
    57841 cagggaaatt agatgagatc gttgatgcat tgatcatgta tgatcaaaca tcaaaactgg
    57901 aagctatgca aaatgggtaa aatgacctat cgagaagtcc tgtcacgggc ttcttctttt
    57961 ttagaggatc atgggaaaga agcatatagt atccaatttt tatttttgga acgaaaacaa
    58021 tggaaaaaac ttgattggct ccttcatatg aacgaagaga tttcagaaga agaacagcgg
    58081 ttgatcgaaa ccgatctgcg acttctgctt gcagatcatc cacctcagta tttgctagga
    58141 tatgcagatt tttatgatca tcggttaaag gtaactgaag caacactgat ccctcgacca
    58201 gaaacagagg agttagtcga atggtgttta gacgagacgc ctggtgtacc gttagaagtg
    58261 atagatattg ggacagggac tggagcaatt gctattagtt tgaaagcagc aagaaaaaat
    58321 tggcatatat ctgctgtgga tctttcagaa gaagcattag aagtagcgaa agaaaatgct
    58381 cagaaagaag gaacgaaaat cagtttttat catggagata cattaggacc agtaatggat
    58441 cagcaatttg atgtgattat ttctaatccg ccgtacatca gtcggaatga atgggaactg
    58501 atggatgaaa gtgtccggtc attcgaacct aaaatggccc tttttgctga gaacgatgga
    58561 ttagctattt atgaaaaaat agcagttgaa gcaccaagcg tactaaaatc aaatggaaag
    58621 atttttttag aaatcggatt tcgccaaggt gaagcagtaa aaaaaatatt tcagcaagca
    58681 tttccagata aaaaagtggc gataaaaaag gatctttttg gcaatgaacg aatgatccaa
    58741 gtttatggtt gaagttgtgg atgaaaaatt ttttatttgt ttttttatta taaaaaaaca
    58801 cttatttgac aaggatgtaa acgacttttt ggtttatcaa caagtttatc cactattgac
    58861 aaatagatta tccacaaaca gtggaaaact tgaggataaa ctcagacaaa ataagaatgt
    58921 atgaaaaagg ggttgtggat aaactgtgga aacgaaacga tatgatgaaa cagaattgca
    58981 agaggcagcc gaagcactga aagaaggaga acttgttgct tttcctaccg aaacagtata
    59041 cggattgggc gccaacgctc ttttaccaaa cgctgtcaag aaagtgtttt ctgtaaaagg
    59101 gagaccgcaa gataatccat taatcgtcca tgtcgcctct tttgaacaag taaaagagta
    59161 tgtggacaac tttcatccgg cagcagagaa gattgtgaag aacttctggc ctggaccatt
    59221 aactttgata tttaagatca aaaaagacac tttgccctca gttgtgacgg gaggattatc
    59281 cacagctgca tttcgtatac cggataacaa aaaaacacta gaagtaatcg aactttccgg
    59341 tgttccgctt gtcggtccaa gtgcaaatac gtcgggaaaa ccaagtccaa cgactgcaga
    59401 tcatgtatat catgatctac aaggaaaaat taccgggatt attgatgatg gagctacgcg
    59461 aattggtgta gaatccactg ttcttgattt aagtgatcca acagccatgc ctatgatttt
    59521 acgaccaggt gcagtaacga aagaacaaat cgaagcagtc atcgaatctc ctgttgctat
    59581 tgatcagcat cttgtaaaag aaaatgaaac accaaaagcc cctgggatga aatacaaaca
    59641 ctactcaccc gatacacgtg ttctgatggt cagagatggc gattggtcaa ctgcagttca
    59701 atgggcaaaa aataagaaga tccgtgcagg tgtcatagca agccccgaaa ttgcggatca
    59761 agtgcggaca gatactgcag ctgtttatat gtataacgac aactcagtag aagcggcggc
    59821 taaaggattg tttgcaggtc taagaggatt agatgaacca acacttggat tagatttgat
    59881 ttttgtacaa gtgtatccag aaactggatt ggggaatgct tacatgaatc gcttgaaaaa
    59941 agcagcagga caaaattatt ttgaaaaatg atcaattggt tattgtcgca ttcttttagt
    60001 atgatacaat gctatcaagt aaatcttcta tcagtgaacg aaaaactaag cgtaactaat
    60061 cgtaatagaa gagatacgaa tatttggcaa tttgtttttg atgggaaaaa taaaattgta
    60121 ggaaaataaa aaggagctgt tgtttgtggt agattacaaa acgtttgacc ctgatctttg
    60181 ggctgcgatc gccaaagaag aagaaagaca agaacataat ttggagctga ttgcctctga
    60241 aaactttgtc tccgaagcag tgatggctgc acaaggaagt atcttgacaa ataaatacgc
    60301 agaaggttat ccagggcatc gctattatgg tgggtgtgaa tttgtcgata tcgttgaaaa
    60361 tttagcgatt gaccgtgcaa aagaattatt cggagcgaaa tttgcgaacg tccagcctca
    60421 ctccggctct caagccaaca cggctgctta tctcgctttg gtagaaccag gggacacgat
    60481 cttaggaatg gatctttccg caggcggaca tctaacacat ggatcaccag tcaacttcag
    60541 tggcaaaacc tatcactttg ttgcttacgg tgttgatccg acaacagaag ttattgatta
    60601 taatgtggta cgtattttag ctagaaaaca tcaaccaaaa ttgatcgtag caggtgccag
    60661 tgcttacgga cgaacgatcg actttgcgaa attcagagaa atcgcagatg aagtaggcgc
    60721 aaaactgatg gtagatatgg cacatatcgc aggattagta gcagcaggac ttcatcctaa
    60781 tccagttcct tatgcagata tcacaacgac taccactcat aaaacattgc gtggccctcg
    60841 aggcggcatg attttgacaa acgatgaagc attagcaaaa aaaataaata gtgcggtatt
    60901 tcctggtatt caaggcggcc ctctagagca tgtgattgcg ggtaaagcag tagcattcaa
    60961 agaagcatta gatcctgcat ttaaagaata tagtgaacaa atcatcgcca atgcaaaagc
    61021 aatggtcaaa gttttcaatc aagcaattgg aaccagagtc atttcagggg cgacagataa
    61081 tcatctgatg ttgatcgatg ttcgcgagtt aggtataaat ggaaaagaag cagagagcat
    61141 tttggacagt gtgaatatca ctgtaaataa aaactcgatt ccttttgaaa cactaagtcc
    61201 attcaaaaca agcggtattc gaatcggcac gccagcaatc actactcgcg ggttcaaaga
    61261 agaagatgct gtaaaagtgg ctgaactagt tgtcaaagca ttacaagcaa aagatgataa
    61321 cgctcaacta gacgaagtca aaacaggtgt acgcgaatta actgaaaaat ttccgttgca
    61381 taaaaaataa aaatttaaat tccattagta aacagaaaag ttcaatgttg ggcttttctg
    61441 tttatttttt gggaaaacgg attctttgac aatatcggtg atgctctcta gtatatgagg
    61501 tgtaatttta gtcttcacac taaatctcag ggaggaaaag tcatgactga tcgtatctcg
    61561 ttagaaaagg aagcagtagc attcagtgat gcaaatgcgc ctcatccgcg tatctatgaa
    61621 ctagcaccgg aagaaggaag agttttacta gaagaagtac aagaatcacc tgtgaacaaa
    61681 tattccgtcg atattgaaga cttgatggtg gatactggcg aatggggaaa actcaatatt
    61741 cgtctcgtac gaccagaagg gaatatggat aaattaccag ttatttttta tattcatgga
    61801 gcaggctggg tttttggcag tgctcaaacc catgataaat tgatcagaga acttgcggtt
    61861 cgtacgaatt ccgtcgttgt ctttccagaa tatagtcgat caccagaagc aaaatatcca
    61921 acagcaattg aacaaaatta tgctgtgttg caacagctga ataaattagc caagtcaaaa
    61981 aatctggatc tcgagaatct aatcgttgca ggagattcag taggtggtaa catggctaca
    62041 gtcatgacga tcttaacaaa agaacgaaag ggcctcccaa tatccaagca attgctgtat
    62101 tatccagtca caaacgctga ttttgataca gattcctatc atgaattcgg agaaaactat
    62161 tttttgacaa gagaaggaat gatgtggttt tgggaccaat atacgacgaa tgaagaagag
    62221 cgtgctgaaa tcacagcttc gcctttgaga gcaaatataa atgaactaaa tgatcttcct
    62281 tcagcaatga ttttaacggg agaagcggat gtattaagag atgaaggtga ggcttatgcg
    62341 cgcaaacttc gtgatgcagg ggttcctgtt acacaagtac gcttccaagg aatcatccat
    62401 gattttgtca tggtaaatgc tatggatcag acaaaggcaa cgcgtgcagc aatggcatta
    62461 tctacttctt ggatcaagaa aaaagaataa gctttgcttg tccgcaggaa aacttctttt
    62521 caaaaacgga ttttcttagg aaaaatggcg agtttgacta gtcaatcact tgattttgtt
    62581 ttacaattaa aaagaaacga cagaacaaag gagagcttaa aaaatgggca aatttcaagt
    62641 gatcgatcat ccattgattc aacacaaact aactattatt cgggacaaaa actgcggaac
    62701 aaaggttttt cgtgaagtag tcgatgaaat cgctatgttg atggcatatg aagtatcacg
    62761 ggatatgcca ttggaagaca tcgtgatcga aacaccgatc actgaaacaa cacaaaaaac
    62821 gttgtctggt aaaaaagtag cgattattcc aatcttgcgc gcaggtattg gaatggttga
    62881 cggtatcttg gaattgattc cagctgctaa agtgggacat atcggtcttt atcgagatga
    62941 agaaacattg gaaccacatg aatatttcgt caaacttcca gaagacattg atgcacgtca
    63001 gttgtttgta gtagatccaa tgttggcaac aggcggatca gcaatcatgg caatcgatgc
    63061 attgaaagaa cgtggcgcgt cgaacattaa atttgtttgt ttggtagcag cacctgaggg
    63121 agtaaaagct ttgcaagaag cgcatcctga tattgacatc tatacagctt ctctagatga
    63181 aaaattgaat gaacatggtt atatcgttcc tggactaggg gatgcgggtg accgtttgtt
    63241 tggtacaaaa taagtaaata cgaaaaaggg tgtgacacaa cttttgtcac atcctttttt
    63301 ggtcattttt aacgtttgat gcagaacagc tttttcacag ctttcgtttg catataaaaa
    63361 aaccatccca aaaatgcaga gatggtcaaa ttcaaatctt ttttagttct ttgtagttaa
    63421 cgattactac acccttactt tgcgtcatca agttcgcatt ttctgcagtt tcatcaatct
    63481 caacaacagc ggaatttttc aattgctttg aaatcgttcc agtttgcgac ttgccgtgaa
    63541 ttgtgaacat tacgtgagta ttgatcttga atttttcacc aatttccgat ggtacgatag
    63601 ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc tgcctttcac atcaattata
    63661 ccacgtttta tgaaaaatat catgaaatta aactaatttt ccatcggact ctttacaatc
    63721 agccaaatag agtatataat ttcttttgga taaggccagc ttagctcagt tggtagagca
    63781 acgcactcgt aacgcgtagg tcgtaggttc gatccctata gctggcatta aaagagaaga
    63841 cagatatatc aaggattttt aatccattga tacatctgtc tttttgttta aaggaaataa
    63901 agtatgcttt cttagcaata gagcatgtat tatttctata aaaatttagg agaataaaac
    63961 gactagcaat atagctgtga aatcaaatat cacatgaact gcccaacttg taatgatcga
    64021 gttgctttta atggcagctt ggttgaataa caatcttgcc accccttgta tcaaaaggat
    64081 gtgtgctaca gtgtgtaaaa tatttccatt atcataagta ctgtaatgga taaatccaaa
    64141 gatgattgct gacaagaaac tagcgacagg aatgctgaac ttactagaaa aaatagcgag
    64201 aaaatacact gagaacaatt cttctccaag tatcatgata ggcaaaataa taaataataa
    64261 cgggctgaaa gcctcattta ttggattgcc tgttaaaccc catttcaaaa tttgttgcaa
    64321 aaataccgag actgcggcac tgattactac attgataaaa aaatatttgg ctatattttt
    64381 gacaggagcg acaggtttcg agaacatttt tttcgtatct tttttaccaa aaacaagtgt
    64441 aaatgctata aatccagtga tagttaaaga aaaaatgcta ataatggatg gcgcaaatag
    64501 caaccctaac aaaccgagta caacgacaat agtgcccaat atcccttttt taagttctat
    64561 tttttttcct aaaatgttat tcatgataat aactcctact attcagtttt tgaagataga
    64621 gtcagtatac atgaagagtt aaaataggca atgttttttt atggatattt gtattgctaa
    64681 aaatttatct tatatattca ttttgattat actactttta tttgtaaaaa ttttctagca
    64741 aaaccaagtg cttgcaactg attaagcgta caatttttta gatgttccga gcgtacttca
    64801 atcatttcaa aggagttgct acttaaatcg atatttttta aatcagtatc aaaaaagcta
    64861 gcattttcta aatcattagt agaaaacatt attttagtga gggaacagtc tacaaaatct
    64921 gcgcttgata agacagagtg ctcaaaggta ctttctttta tgtttgaacc agataaattt
    64981 acatagtcca ttttacaatt ctcgaataac gtgttcccaa tataactttc catcaattta
    65041 gtacctgtta atttacaatt gacaaatttc acgcgatgca acatgctttt tgtcaatata
    65101 cagcctgaaa aatcacagtt ttcgaatata cagtcggtaa tctccattct ttgaaaatca
    65161 ttggaagcaa aaaatgcata cttgaaaata gatttgtaaa taattggatg gcagattatt
    65221 tggttttcga tgacctcttt ttgtgaaagg atattataca gttcaaaatc ttcgttttca
    65281 tagatatgaa aaaaggatcc tttattcaag cttgcttcat taactttagg aaaaatcata
    65341 tttaattacc tccattttca taaagtcata ttaacaaaag ctaaaccaac ttacattagc
    65401 agaaaatgag ataggttttc gcctttttcg ataacgatag tgtcgctatc tcttgaaacg
    65461 ttcttagaat tgccctgaga gacacaaatt aggcgtaatt ctaataaaaa tcttagaata
    65521 aaaattttta ctgatttaat ttagaatgtt tttaaaataa aaatcgttgc cataacagtg
    65581 tattgaaagg ttttaacaat ccagtaatga tttaaaaaaa tctagatgta gcgtatactt
    65641 tgtttataga atgtagtggg aagaaatata tttttgggag gaaatcgtga tgtctaaaag
    65701 tgaagaaata gaaaacatgg tactaaaatt atatgatctc gttttaaatc cagagatcaa
    65761 agaaaaggaa cgtatacttt tgatagacgc aaagacaggc ttagaaaaag gtcagtatta
    65821 tcctaaagtc ataaataatt tggaacgtag tttacgtccg ttagcaatca ggggagaatt
    65881 gtctaaacca gtatcaccgt tttatatgga gatatcaaca ataggaaaat ttgaaaaaga
    65941 attaggtcga ggaatggctt cggcaccgat tacttttggg catttataaa aacgggttat
    66001 tcatctcaat gaattaaaaa gtatagtcaa tctcataatg caggctaggt tcatttattt
    66061 gaacagagtc tgtatttttt tattctaaat aaagaaacag atgtttcttt atattgtatt
    66121 ttatccttta attaacacaa aaacagctag tataaactaa taatgttaat taaaagtgta
    66181 ttttatgaaa ctatcgttat ttattaatgt tgcatttttg taagttaaaa attggaaata
    66241 tagattgaaa aaatgaaagc aataagtaaa ataaaatgaa tatatgcgaa aaaataattt
    66301 acattaggaa aaaccttgta aaaacagttt tttggcatat ttatttttta taaatatttg
    66361 taaaataact atcaattttt tagttaaaag attggaaatg agtgggggag gaattgaaga
    66421 aaggattttt gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa
    66481 aaaatgaaaa agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca
    66541 tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact
    66601 tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg
    66661 tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt
    66721 gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa
    66781 aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt
    66841 aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc
    66901 gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact
    66961 gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga
    67021 gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc
    67081 acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat
    67141 ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa
    67201 gagggaatcc aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact
    67261 gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg
    67321 agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct
    67381 tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa
    67441 aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa
    67501 tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat
    67561 gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct
    67621 agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg
    67681 attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca
    67741 gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa
    67801 atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt
    67861 acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact
    67921 tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa
    67981 gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa
    68041 aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca
    68101 tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta
    68161 gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt
    68221 ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac
    68281 aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg
    68341 gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag
    68401 aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat
    68461 acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag
    68521 ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct
    68581 gtaatcattg gtttattgat agtaactatc tgtgctggga tattcttaca aaaatataaa
    68641 aaagtttagt tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaat
    68701 tccgatttat aatcgaaaaa agaagcattt ttatatatgc ttcttttttc gtagaatcaa
    68761 taaatatgga tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta
    68821 gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa
    68881 aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg
    68941 gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag
    69001 tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca
    69061 cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg
    69121 gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta
    69181 attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga
    69241 tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa
    69301 aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca
    69361 gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc
    69421 ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta
    69481 cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga
    69541 gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt
    69601 ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga
    69661 ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa
    69721 cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt
    69781 gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt
    69841 ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt
    69901 gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc
    69961 ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag
    70021 ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg
    70081 caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa
    70141 gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga
    70201 gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc
    70261 tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta
    70321 gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt
    70381 gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta
    70441 ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcgtaat gaaacaagtt
    70501 gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag
    70561 aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca
    70621 gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt
    70681 atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc
    70741 aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag
    70801 ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa
    70861 cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt
    70921 gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagcgatt
    70981 gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta
    71041 gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta
    71101 acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta
    71161 aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat
    71221 tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag
    71281 ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg
    71341 ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt gatttagcca
    71401 aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga
    71461 aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc
    71521 cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga
    71581 tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg
    71641 cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga
    71701 tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga
    71761 aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac
    71821 aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg
    71881 cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc
    71941 aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta
    72001 cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg
    72061 aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac
    72121 ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa
    72181 tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca
    72241 acctccttta catattttta ttttgttgtg gaaggggttt cgtgttattc taaaagagga
    72301 acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt acctcatttc
    72361 caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga gcttggcaag
    72421 aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga aagacattta
    72481 aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa gatattgctt
    72541 taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca cggatttttc
    72601 ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg
    72661 aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat cttccacaag
    72721 cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat ttcactcatt
    72781 atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt agtttgaatg
    72841 aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca ggtgtgacag
    72901 acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca tctgtcatca
    72961 ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa
    73021 attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga
    73081 tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt
    73141 tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat
    73201 ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc
    73261 cattaaacgg agttggtgct ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag
    73321 aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt
    73381 gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt
    73441 accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag
    73501 ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca
    73561 aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt
    73621 tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg
    73681 aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac
    73741 tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg
    73801 gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa
    73861 gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt
    73921 tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac
    73981 taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct
    74041 gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg
    74101 ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat
    74161 cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc
    74221 aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt
    74281 gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac
    74341 atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg
    74401 gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta
    74461 tttagcagca gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta
    74521 tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga
    74581 tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga
    74641 agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag ctgcagaata
    74701 tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc
    74761 agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat
    74821 ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga
    74881 aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc
    74941 agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac
    75001 tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac
    75061 tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac
    75121 aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga
    75181 tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa
    75241 atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat
    75301 catcgaagca caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt
    75361 ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc
    75421 gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc
    75481 taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta
    75541 caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc
    75601 cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt
    75661 aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa
    75721 acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt
    75781 agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg
    75841 gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa
    75901 agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat
    75961 cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat
    76021 gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg
    76081 ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac
    76141 agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc
    76201 aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg
    76261 gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg
    76321 cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac
    76381 gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc
    76441 aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta
    76501 atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt
    76561 tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa
    76621 agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca
    76681 attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt
    76741 atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa
    76801 aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg
    76861 atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga
    76921 tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct
    76981 gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg
    77041 ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt
    77101 cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact
    77161 ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt
    77221 ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc
    77281 tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc
    77341 gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc
    77401 gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc
    77461 aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat
    77521 ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat
    77581 tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag
    77641 atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat
    77701 aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa
    77761 ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca
    77821 agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg
    77881 gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga
    77941 taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca
    78001 tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca
    78061 ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt
    78121 ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca
    78181 gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag
    78241 tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca
    78301 atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc
    78361 aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct
    78421 aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg
    78481 gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct
    78541 gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct
    78601 tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga
    78661 aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg
    78721 ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt
    78781 tcaatatttc cataagttga attacccccg taccagagac tggcgacaac ctctatttcc
    78841 tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc
    78901 agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt
    78961 ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt
    79021 tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag
    79081 gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat
    79141 aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt
    79201 cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa
    79261 ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag
    79321 caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc
    79381 ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga
    79441 taaataatga aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt
    79501 cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca
    79561 gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga
    79621 cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag
    79681 aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc
    79741 ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa
    79801 agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag
    79861 gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg
    79921 acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt
    79981 atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct
    80041 gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat
    80101 aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt
    80161 acttcttgaa tttttgtgaa attccaactt atttccgaag aagctacttt tggaacaccg
    80221 tgtaccagag gttgtgaaaa agtcgcttag tttcgagtag taagaaaaat tttgtgaaaa
    80281 tggctttcta catttttgca taaatttggc ttaataccga gaaactggct tttgaacacc
    80341 gtttattcag ataagggact gtgacaagac ttttgtcaca gtttcttttt gtttattctt
    80401 ttttatcttt gtatggcaat aatagacaat ataacgaaaa ttatttaatg gcttttggaa
    80461 gaccatggaa aaccttgtac aaagctattt tttctgaaaa attccttgaa acctcacgaa
    80521 aaaatcatta ttttatgaaa aagacaaaaa aaagtgttga ttttttaata attattcatt
    80581 aaactcttaa acaacttcag aaaatattaa ttattctgaa aattccaata aaggggtacg
    80641 taaaatgaaa aagaaattta catttggttt ggttgcggta tgcggccttg tacttgctgg
    80701 atgttatggc ggcagttcag ccacaagcag tagttcagca aaaagcggtt cagcagatgg
    80761 aggaggcgta tttaacttag tcgtgcctca agaaatgcct acagctgact tatcagttgc
    80821 aacagataca atcagtttta cagcattgaa taacgtttat gaaggaattt accgtcttga
    80881 tgaagacagc aaaccacagc cggcaggagc tagcgagttg gcagaagtca gtgaagacgg
    80941 attaacttac aaaatcaaat tacgagaaga cgcaaaatgg tcaaatggtg atcctgttac
    81001 agcagcggat tacgtttatg gctggcaacg aacagtagac ccagctacag cttctgaata
    81061 tgcttacctg tatgcgccag ttgaaaatgc agaagacatt acagctggaa agaaagataa
    81121 atcagaacta gggatcaaag cagttggtga ctatgaatta gaaatcaaat tgacgaaaca
    81181 aacaccatat ttccaatatc tgcttgcttt cccatcattt ttcccacaaa aacaatccgt
    81241 agtggaagaa aatggggatg cctatgcatc tgcaagtgat aaagctgttt ataatggacc
    81301 attcacatta gaagattttg atggaccagg aacagataca gaatggactt ataagaaaaa
    81361 tgatcattac tgggacaaag atacagtaaa actttctgaa atcaaagtca gtgtagtaaa
    81421 agaatcttct actgcattga acttattcaa agatggccaa gcagatgacg tgatcttgtc
    81481 tggtgagtta gcacagcaaa acgcaaacga tccagcttat acttctgtta aagaagcgcg
    81541 gacaagctat atcgaattca atcaacgtga aaagaattca ccattcaaca atgtgaacct
    81601 aagaaaagct atttcttatt ctatcgatcg tgaagcttta gtaaaacaag tactaggtga
    81661 cggatcagtt gtctctacag gattgatccc atcagatatg gtaaaaagtc caacaacgaa
    81721 cgaagacttt gcaaaagaag ctggaaaatt agtttcttat gacaaagata aagcaaaaga
    81781 atattgggaa aaagcgaaaa aagaattggg cgttgattct ttagaatttg atttgatggc
    81841 ttctgatgat gattcatcga aaaaagtaat cgaatatgtt caaaactcaa tccaagaaaa
    81901 cttggatggc gtaacagtca aaccaacacc tgttccattc tctgtacgtc ttgaccgctc
    81961 aacttcgggt gattttgacg cagtactagg cggatgggct gctgactatg cagacccaag
    82021 cagctttact gatttgtttg tcacaggtaa ttcatacaac cgtggtcgct ggagcaatgc
    82081 agaatatgac gaagcagtta aagcagcttc atcaacagac gctggcgatg aacaagcccg
    82141 ttgggatgat ttccaaaaag caatcaagat catctctgat gatatgggtg tcgttccggt
    82201 gtaccaaaaa gcagaaggtc acctagtgaa tgaaaaaatc aaaggtatcg ttcaccatgc
    82261 ggcaggcgct tcatgggatt acaaatggac atacgttgaa gaataatcgt taataccgtg
    82321 agcatgagaa aaaacgaggc tgcggcaaat gtcgcaacct cgttctcata ttcacttatc
    82381 ctaacaaaga aaaaagcatg aaaatagcag aaagagcaat tagtattccg gcaatgatca
    82441 gccaaaagct gtacataccg tagctggcag acgagagcga ggaaaattct ggttttttct
    82501 ttcggttcct tgttcgtgca agatggaccg atcgctgaaa atgatcgaag aaacccgaag
    82561 aaaagaccca taaaaaaccg ccgataatca gaaacggcaa tgaccataag aatagatcat
    82621 tagataattg aatgagtgta aaggaatggt tgatgagatt ttttattaaa ataaccagaa
    82681 tcagtagaaa agctactgcg taggggatag attttttttt catttgattg ctcctttatt
    82741 tttggttcat tatctaataa tgtagcgaaa catctgtcag aagtcaaaag ctagggggaa
    82801 acattagtga atagttatat caaatatgta ttaaaacgtg tctttttcat gatcatcacg
    82861 ctttggttaa ttgcaacaat cacctttttc ttgatgcaat tgctgccggg cacgccatat
    82921 acgaatcagg aacgtttgag tcctgaaaca atcgagatgc taaataaaca agtcggtttg
    82981 gataaaccag ttatcgtaca atatgggatt tatctttcta atttgcttca aggagatttt
    83041 ggaatttcct tccaatttaa aaaccagcca gtcgcacatt tgttagctgg aagaatcgga
    83101 ccatccttgc agttagggtt acaggcaatt atctttggaa cagtcctagg gacgatttta
    83161 ggaacgatct ctgccatgaa gcaaaataca tgggcagata catcttctac actcgtagcg
    83221 atattaggtc gttccattcc aaactttgta tttgctgttt tattacaata tatctttgca
    83281 atcaaattac aagttttgcc aatcgcaaaa tgggatggct ttgtgtacac aatcttacca
    83341 acaattgcgt tggccatgtc gccgcttgct gactctgctc ggtttatccg tacggaaatg
    83401 gtggaagtac tgcatagtga ttatgtggaa ttagcaaaag caaaaggatt gagccgttgg
    83461 gaaatcgcct ttaaacatgg tcttcgaaat agtttgattc cattgatgac tttattagga
    83521 ccgttagctg tagcattgat gaccggatca ctggtggtag aaaatatctt tgctattcct
    83581 ggtatcgggg aacaattcgt taagtcaatc acaacaaatg attatcctac gatcatggct
    83641 gtaacgatcc tttactcatt tatgctgatt tttgttattt tgatcgtgga tctattgtac
    83701 ggtctagttg atccaagaat tcgtgtttca gaagggagcc gaggataaat ggaaatgata
    83761 ccaaagcgtt atgaaacagt tgcagatatc cctgcggatg aatttcttcc attacaaaaa
    83821 aataccgaag aagaacgtga acagattact gcaccttctt tgaattttat ccaagattca
    83881 tggcgccgtt taaagaaaaa caaagctgcc gttatttcaa tggttttact attggtcatc
    83941 attttgatct ctgtgatcac aatctttgtt tctcctcatg atccaactgc ccaaaacgtg
    84001 gcttatatca atttgcctcc tcgtattccg ggaatcaata tcgatggatt gaatggaaaa
    84061 gcaatggttg gtggtgaact tgttgataaa tatgctcaag caaacgtccc ttcaaacgta
    84121 aatttctttt taggaacaga cggtttggga cgtgatgtat taagccgctt atttatggga
    84181 atgagaatct cgttgctgat cgcatttatt gctgcattac tagatgtaac gattggtgta
    84241 gcttacgggt tggtttctgg tttgttaggc ggacgtgtcg ataacgtgat gcaacgtttt
    84301 ttggaaatct tatcaggaat tccaaacctc gttgtcatga ttttgatgtt ggtcgttttt
    84361 gaaccaggaa ttttttcaat cgtggctgct atggccatca caaactggat accaatggca
    84421 cgtatcgttc gtgcacaaac aatgaaatta aaagatcaag aatatgtttt agctggattg
    84481 acattaggtg aatcaagaat gaaaattgca ttcaaacata ttttgccgaa tatctctagt
    84541 gtcattatta ttcaaatgat gtttagtatt ccatctgcta ttttcttcga agccttttta
    84601 agctttatcg gtttaggtct tacgcctccg tcagcttcat taggaacgat gttgagcgaa
    84661 gggtacaaga cattcctgta tctgccttat ctattatgga ttccggcagt aactttatcg
    84721 gttatcatga ttggctttaa cttacttgct gatggattgc gtgatgcctt tgatcctaaa
    84781 atgaaagagt gaagacgatg acgaaaattt tagaagtgaa agacttagaa atctcttttg
    84841 atacgtatgc tggaaaggta cgagcgattc gcggtgtcaa ttttcatttg aataaaggag
    84901 aaacattggc gatcgttggg gaatctggaa gtggaaaatc agtgacgacc agaagcatca
    84961 tgcgcttgct gtcaagcaac gccaatatag atgctggtca aatcctcttt aaaggtaacg
    85021 atatcgttgg aaaatcagaa agagagatgc aaaaaatccg tggtaaagaa atagcgatga
    85081 ttttccaaga tccaatgacc tctttagatc cgacgatgcc cattgggaag caagtcgctg
    85141 agtcccttag aaagcataat aaagtttcaa aaaaagaggg acaaaaagct gcattagaat
    85201 tgcttaaatt agttggaata cccgaagctg agaaacgtat caacagttat ccgcatcagt
    85261 tctcaggagg tcagcgtcaa cggatcgtga tcgcgattgc actggtatgt tatccggaaa
    85321 tcctgattgc tgatgaacct acaacagcgc ttgatgtgac tattcaagca cagattttgg
    85381 agctgctgaa agaaattcag acaaaaatcg atacatcaat tatctttatc actcatgacc
    85441 ttggtgtagt agcgaatgtc gctgatcgtg tcgctgtcat gtatggagga agaatcgttg
    85501 aagttggaac atcggaagaa attttctaca acccgcaaca tccctatact tggggactgc
    85561 ttggttcaat gccgacatta gatagtgcga acgatcgatt gtatgctatt cctggttctc
    85621 caccggattt attgaacccg ccaaaaggag acgcattcta tccaagaaat gaattcgcga
    85681 tgaaaatcga tgcagaacaa gaaccaccat tttttgaact atcaaaaaca cacaaggcag
    85741 caacttggct acttgctccg caggcaccca aagtgacacc gccagaagaa atccaacgtc
    85801 gctgggctat ctttaaagag aaacaaaaac atacttacgg tcaaggagga attgctgatg
    85861 ccaaataaac tacttgaagt taaaggattg aaacagtatt tcaatgtagg aaaaaagaat
    85921 gaagtacatg ctgtaaatga catcagcttt cacatttatg aaggagaaac attcggttta
    85981 gttggagaat ctggaagcgg aaaatccact actggccgta cgatcattcg cttgaatgaa
    86041 cctactgatg gtgagatatt attcgacggt caagatataa cgaagatcaa agataaaaaa
    86101 ggtttgacga aattccggca tgatgttcag atgatctttc aagatccata cgcttcgttg
    86161 aatcctcgta tgaaagtcag agatatcatt gccgaaggaa ttgatgttaa tggactagct
    86221 aaatcatctg aagagcgtgc aaaaaaagtc gatgatctat tgagaactgt tggattgaat
    86281 ccaagccacg gtactcgtta tcctcatgaa ttttcaggtg ggcaacgcca gcgtattggt
    86341 attgcacgag cattggcagt aaaaccgcgt tttatcattt gtgatgaacc aatttctgca
    86401 ctagatgtgt cgatccaagc ccaagtggtc aatttgttgc aagatctcca aaaagaacat
    86461 caattgacct atctatttat tgcacatgat ttgtcgatgg ttaaacatat tagtgaccga
    86521 atcggtgtaa tgcataacgg cttgttatta gagatgggaa caagtgatga aatctataat
    86581 cacggtgttc atccttatac agaaagtttg ttatctgcta ttcctctacc agatccagac
    86641 catgagagaa aacgcagaag aatcaaatat caacctgaac cagatgacgg acaagtacgt
    86701 caacttcgtg aaatcgcacc agaacatttt gtctatgcaa ctgagcagga agtcccatat
    86761 tacgcaaaaa aactaaaacg acaaaaggaa tctcttctgg ttgctaacta ggagggacat
    86821 agatgctaaa agcttttaaa ttccgtatgt atccgacaga agaacaaaaa cagcaattga
    86881 ttcgtacttt tggatgtgct cgatttacct ataaccattt gctgaaaaag cggcagaaaa
    86941 gttggcaaca aacaggagta gccaattttt cgctcacacc cgctacatta aaaaaagaat
    87001 acccattctt aaaagaagta gacagtcttg ctttagcaaa tgcacagctt aatttggatc
    87061 gggcttttcg caattatttc aagggacgtg ccagcttccc aaagcttaaa acgaaaaaaa
    87121 gtatgtggca atcttatacg accaacaacc aaacccgtac gatctatctg gaaaatggct
    87181 acttaaagtt accaaaacaa aaagaactaa tcaagataaa tcagcatcgg ccagttgaag
    87241 gatcgattcg ctccgctacg atttctgctc gatataatga agaattttat gtcgcactac
    87301 tttgtgatgt ttcgccagta aaaaaagaat ctttagctaa atggatcggg atcgcctatc
    87361 atccgaagac attgatccaa acatctcgac ctctagaagt aactttacca aaatttcacc
    87421 agacagaaga aaagctgcaa catgcacagc ggaaattgaa tgtgaaagtc cgctccgctc
    87481 accatagaaa gatcaggtta gatcaggcaa gcaattatca gaaacaaaag agaaaagtaa
    87541 tggatcttta tctgaaacaa aagaaccagc gagaagatta tcttgaacaa ttatctggga
    87601 aattggtcaa acaatatgac tacttatttg tagaatcttt tccaaaagaa gaggcacatg
    87661 ctgatttttc tattcacgac tggcataaat taataacaaa attgcgttat aagtcccaat
    87721 ggtataataa aaaatttctt ttgatcaata ccgacggagc agaagaaagc aattccgtaa
    87781 gaaagagcca agtagtagaa aaaatgggca gacattcggt tatcaaggga taaaaaatga
    87841 atgaaactga taggagcgtt agggttagcc ttgttaataa aaagtcttaa tgacttttgt
    87901 ttctaggaat ttttcttttg aagaaaagct cactaattat gacaaaccaa taaaacaata
    87961 gcagaaaaag ttcgtacgag ctttttctgc tattgttaat tttttattca gaaagtttac
    88021 gaatacatca aaattctgaa acaaaaaacg taactcacaa agagttgttt ttgcgttttt
    88081 tcttgaatga ttaggtctga atcttttgct cacaacctta ttcatggtgc cccggcttga
    88141 gctgatagat ttttgaagga cggccgattc ctacaggacg ttcaccgatc tcatcgaaat
    88201 attggagcat agctttctta aaattggaat gatcgatcgt tttataatcg atccctaaga
    88261 atttagcaaa tacttttcga gcttccgtaa tcgtgaaatc tttacctaaa acttgtagaa
    88321 cttgcggctc gtgttccatt ttattgacta cacggttgaa tgctttcaag atgatttgac
    88381 tatggtcaaa agccaaagta tctttgccag aagactctcc agtgactaaa tcaagcgaga
    88441 tagccacttc tccgctagat aaatggatct tgttactatg acgttctagt gtgaaccagc
    88501 gtacttcctt ggcatcatct ccggcgatta gcggttcttc tccaataaag gctaagtagc
    88561 tgactgtgac gacccagcct cgcggatctc gattaggcgt actgaatgta tgcagttgct
    88621 caatattttc ttttgaaata accacaccag tttcttcctt tgtttctctt aagacgcttt
    88681 ctccagttga ctcatccttt tggacaaagc caccaggcaa tgcccatgaa tttcgataag
    88741 gatgcccttt tctttggatc aacaaaactt tcagctgatc ttcttctttg ttatagcaca
    88801 tcagtacgat atcgacagtc aaagagggct tgtcatattc tggacgttcc tgtttttgat
    88861 accagttaag aaattcttct tcacttgctt tatgttcata atataatttt tcttcttctt
    88921 tagaagcaaa acggttcatc tgaccacctc cattgacagt aaaagtactt tttaccttat
    88981 tgtagcagac tctgaaaaaa aaccaaggat ttacttgaaa aatctaggtt atcatttaaa
    89041 tatgaaaaat aaatggaagt atgagattga ataaaaagaa aagcagataa aacggcaata
    89101 ctttataccg ctcttcacga atggcaaaga agccacaaat aacgatatat acgtcaatga
    89161 taaaacgcaa aatccaagct gctgtcataa aactgttcaa atgcccatgg aggttaaaat
    89221 aaccatacat acgaatataa aaatagctga atattaaatg tgctaatgaa agaaaagcaa
    89281 tactaagtgt aaaaaaaaga tgtttcttct gcatgattgt tccagtccct tactaagata
    89341 gatagtcttt attataccac gtgtgagggg agaaataaaa agccgttttt tagtagatat
    89401 gaaacaaaac aaaaacccgc atttattcaa cctattcatc cttttaaagg atagattgaa
    89461 cgtagataga agtcgtttat ccagtctttt tcattttgaa aaaaagctaa cagcatgctt
    89521 tttttatggt atactagcaa ggaaacgaat tcgcatcatg cgataaagga ggaagagccc
    89581 cgtggcagaa aaggaaactt tttatatcac tacgccaatc tattatccaa gcggcaaact
    89641 tcatatcggc aactcgtata ctacgattgc atgtgacgca attgctcgtt ataaacggtt
    89701 gatgggcttt gatgtgtttt atctcactgg agtggatgaa catggtcaaa aaatcgaaac
    89761 aaaagcagaa gaactaggtg taaaaccgca agaatatgta gataaaatgg cggctgatgt
    89821 caagaaatta tggaaaacgc ttgatatcag ttatgataaa ttcattcgca cgacagatga
    89881 ttaccataaa gcagctgttc aaaaaatatt tgatcgttta ttagaacagg gagatattta
    89941 cttaggtgaa tatgaaggct ggtactctgt ttctgacgaa gaattcttta cagaaacaca
    90001 acttgcagaa gtgtacaaag atgacgaagg taacgtgatc ggtggtaaag cgccaagtgg
    90061 acacgaagta gaattggtaa aagaagaatc atacttcttc cgcatgagca aatacgcaga
    90121 ccgtctattg gaatactatg aagaacatcc cgaattcatc cagccagaat ctcgtaaaaa
    90181 cgaaatgatc aacaacttta tcaaaccagg attagaggat cttgcagttt cacgaacaac
    90241 ttttacttgg ggagtaccag tcaagagtga tccgaaacat gtggtgtatg tatggatcga
    90301 tgcattatcc aattatatta ctgcactagg atatggctcc gaagatgaaa gtctattcga
    90361 aaaatactgg cctgcagatg ttcagatggt cggtaaagaa atcgttcgct ttcatacgat
    90421 ctattggcca atcatgctga tggcccttga tttgcctcta cctaaaaaag tatttgggca
    90481 tggctggcta ttgatgaaag acggaaaaat gtctaaatca aaaggaaatg tcgtttatcc
    90541 tgaaatgctt gtggaacgtt atggtttaga tgcactccgt tattatttgc ttcgtgctgt
    90601 tccgtttggg tcagatggtg tctttacgcc agaagatttt gtttcccgtg tcaattatga
    90661 tctggcaaat gatctaggga acttattgaa tcgaacgatt gctatgatca acaaatattg
    90721 tgatggactc gttcctgact atgcctcact tgtcactcca tttgatagtg aactgtctac
    90781 gacggctgct aacgttgtcg gacgttacca tgatgcaatg gaaaaaatgg aattcaatac
    90841 agcattggct gaaatctggg tgctgatttc tcgtgcaaac aaatacattg atgaaacaga
    90901 accatgggta ttagcaaaag acgaagaaaa gaaagctgaa ttgaatagtg tcatgatcca
    90961 tttagctgaa tctttgcgta tcgcagcgat attacttcag ccaatcatga cagaaacgcc
    91021 agagaagatc ttcaatcaac taggattgga ctctgaaaca atgaatcttg aaggattaca
    91081 ctttggcgaa tttccttcag gaacaaaagt agttgcaaaa ggtacaccaa tcttcccacg
    91141 tttagatatg gaagaagaag ttgcttttat tcaagaaaaa atgtctgaag gtactcaaac
    91201 aaacgaagat acagtcaaat gggatccaga agaaacagaa cttgtttcaa cgaaagaaaa
    91261 acaaatcaaa tttgatgtat ttgaaaaagt agaactaaaa gtagcagaag tcatcaactg
    91321 tcaaaaagta gaaggggcag ataagttgct gcaattccga ctagatgctg gagacagcca
    91381 agaccgtcaa atcttgtcag gaatcgctga attttatcct gatccaagcg aattgatcgg
    91441 gaaaaaagtc gtcattgtag caaatctgaa accacgaaag atgcgtggac aaatcagtca
    91501 aggaatgatc ctttctgcag aagcaccaga tggctcttta caagtaatcg aagcaccaaa
    91561 atccatgcca aatggctcag aaatcgcata gaaattcaaa gggggtatga cactagttgt
    91621 gtcatacccc ctttttccga aatttgagaa gcaattttcg taaattcctt cttattactt
    91681 gaagccaatc atataataca gaaacagaga actatccaaa ataaatccta gaaaaagaag
    91741 tacgagcttc gctgattttt gttaatttag aataatacta ataggaatcg taaaaacaga
    91801 aaaaaagcat aatgataaag ggtcgggcag attttaattg attcatctgg tactcttttt
    91861 tattgtcctt acattcataa agtttgaatg caatgacttt attacatcta tatttatatc
    91921 taacagtagc gttgatgtgt taattataag ttctatcact ttatgttcaa ttagtttgtg
    91981 ctcttatatg tgtgattgaa aatatagata aaaaaacaat catcggcttt gacttcgatg
    92041 attgttttct ttattttgta aaaatcctat cacttgattt tatggtaacc ttttattttt
    92101 gtcttcaaac ggcttggtaa gagagcagta agcagtgcaa atgggatcga aaagacatac
    92161 cagaaaatcg tacgtccgat accataatgt tgatagagct ttgtcaacat cacgtgtaac
    92221 aagatcaacc ctaaaagcaa ggagccagct ataaaccaat gattatccca atgcatgact
    92281 tcactaagtg ccatcactcc aaaaggatag aaataaaaat agatcccgac taaggtcatc
    92341 cgccagttct ttgtaaatgt tccgatattc aataaaaaga aaatcgctga aagcaaaata
    92401 ccgataggcg gaaaaagccc aaccaaaaag gaatacaaca tcccccacaa gatcatcaca
    92461 ggacctttga tcaatgcaac gattgcgatg aacaaagtca gcaatattag tttttgtgat
    92521 aacgttgcaa cagtcattaa tccaaataaa attaatgtga accaatgcca atgggactgc
    92581 tgttgttctt tacgggaaaa atcgacccgg ttggataaat aatctgctaa tttcggataa
    92641 gaacgcttaa aatccataaa ctgttcctcc ttcgtggaaa aggtaggttt agtatacgaa
    92701 aaataaaaaa gaaatacatc cgtcttaaga gggagttttt cactgaaagt ggagaaaaaa
    92761 gaatggtaaa ttaaaatatt ctcagttata ctgaaaatta taccaaattt caaagaagaa
    92821 agaggatggg aatgaaaggc aaactaaaac gagaaatcaa tctgtttggc gcattagcta
    92881 ccgttatggg aaccgtgatc ggtgcaggcg tcttcttcaa aactgctgct gttactgcta
    92941 gtactcagtc tacaagtttg acattgctag cttggctttt gggtggtttc ttaacgatct
    93001 gtgctggatt gacagtggct gaattagcta cagcaatccc ggaaactggt ggagctgtaa
    93061 aatatattga agctgcgtat ggaaaattgc ccagcttttt attaggatgg gcgcaaagct
    93121 tgatttactt tcctgctaat attgctgcat tatcgattat ctttgcgaca caattgacga
    93181 acttattaca attatctacc gattatcttt tgttgattgc agtgatcacc gctgtttctg
    93241 tgacagggtt gaatttgtta gggacaaaag tcggtgcttc tgtacaatca gtgacgttga
    93301 ttgttaaatt aataccaatt gctgtgattg tcatatgggg attattgacc ccaggacaag
    93361 gaactgtcca gttatttcca atcgaagctg gaaaagatgt tacttttgtc gaaggattga
    93421 gcagtgcgtt gttagcaacg ttgtttgctt atgacggctg gttaggtgtc ggtgcaatgg
    93481 caggggaaat gaaacggccg gaaaaagatt tgcctaaagc aattattctt ggtttgagtt
    93541 ttgtaacagt cgtttatcta ttgatcaatt ttgttttttt gaaaactttg ccgattgatc
    93601 atcttgccgg taatttgaat gcagcttctg aagcatcaga cgtcattttt ggaggaatcg
    93661 gtggaaagct ggtcactatt gggatcttga tctcggtata cggtgcattg aacggttata
    93721 cactaactgg gatccgcgtg ccttatgcga tggctttaga agacgagcta cctttcagta
    93781 aacagctgac aaagctttca aagaagttta cggttcctta tgttcctgcg gttttccagt
    93841 tagcagtagc ttgtattatg atgagccttg gttctttcga ctttttaaca gacatgttga
    93901 tttttgtgat gtggctattc agtttactga tttttatcgg tgtctttatt ttaagaaaaa
    93961 aagcgccgga attgccacgt ccatataaag taccacttta tccaatcgtt ccgattattg
    94021 cgattctcgg tgctattttc attttaggta tgacgatgat gacgcaaacg aaattagcat
    94081 tgatcggtat aggtgttacc ttgataggta tcccagtcta ttatcaaaaa aagaaaaaac
    94141 gctcagaaga gtaaaatatt tcaacaacag acagaaaaag tacgaactag gtcctctaga
    94201 tcttcgtgct ttttctattt caataaaaca agttcttcta ttttgatttt tgctacttct
    94261 gcaagtagtg cttatgtgct aaagtaagaa tgaaaaggag agataaacat gatatttgat
    94321 tctcataccc atttaaatgc tgaacagttc aatgaagata ttccagaaac gattcagcga
    94381 gcacaagaaa tgggggtaac gaaaatggcg gtcgtgggct tcgatacgcc aacgattgaa
    94441 aagtctttgc aattaagcca tgactatcca aatatataca gtattatcgg ctggcatccg
    94501 acagaggctg gcagttatac gaaagaaatc gaaaataaat tacaagaaca attgacttta
    94561 ccaaaggttg tagcattagg ggaaattggc ttggattatc attggatgga agacccgaaa
    94621 gaagtccaag ataaagtatt tcgcagacag attgctatcg caaaagaaat gaacctacca
    94681 atcagtattc atactagaga agcacttgaa gacacgtatc aaatcctaaa agaagaagat
    94741 gttcgagata tcggtggaat catgcatagt ttcagtggtg attatgaatg ggcaaaacga
    94801 tttcttgact tagggatgca tatttcattt agcggtgtcg tcactttcaa aaaagcatta
    94861 gacgtccaag aggctgcagc aaatgtacca atcgaacgat tacttgtgga aacagatgcc
    94921 ccttatctgg caccagttcc ttttagagga aagcgaaatg aaccaggcta tactcgttat
    94981 acagtagaaa agattgctga acttcggaat cttccgacag aagaagtggc tgcccaaaca
    95041 tggacaaatg cgcaccgatt atttggtcta agcgaacatg gataagatga agatccaaga
    95101 gattatagtc gttgaaggaa aagacgatac aagaaggctg cgggaagtag ttgatgcaga
    95161 tacgatagaa acaatagggt ctgcaatcaa tgaagagatc ctgatgcaaa ttgaacatgc
    95221 ccaagagact cgtggagtga tcgtttttac cgatcctgat ttttcaggag aaaaaataag
    95281 aaaaatcatt atggaagctg ttcctcaggc aaaacatgct tttttgccgc gcacacaagc
    95341 taaaccaaag aaaaagggaa gccttggtgt cgagcatgca agtgatgctg ctattttaga
    95401 agcattgaaa aaggtcgtga cgccagacat ttcccaaacg gctgtttctg aaatcactag
    95461 acaagacctt ttagactatg gtttaatagc tggggcacaa gcaaaagaaa aacgagaaaa
    95521 actaggtgac gagttgcgca tcggttacac gaacggcaaa caattggaaa aacgtttgaa
    95581 gatgtttcgt attacgccga aagaactcgc ccaagcaatg aaaatagtgg aggaacaaca
    95641 tgagtgaata tcgtgaaatt gcgacaccct ctagaacaaa agagatttta ttgaaacatg
    95701 gtttttcatt taaaaaaagt ttaggacaaa attttctgac tgaaccaaat attttaagga
    95761 aaatcgtaga aactgcgcaa atagatgaac atacgaacgt gatcgaagtt ggaccaggaa
    95821 tcggtgcatt gacggaacaa ttagccaagc atgcaaaaca agtcgtagca tttgagatcg
    95881 atgatcgttt gatccctgta ttggctgata cgatgcaacc ctatgacaat gtgactattg
    95941 tacatcaaga tattttaaag accgatttgt caacagctgt tagagaaaca tttcatgagg
    96001 agcttccgtt aaaagtcgta gcgaatttac cttactatat tacgacaccg atcatgatgc
    96061 attttcttga atcagatttg gttgtggatg aactagtcgt catgatgcaa aaagaagtag
    96121 ctgatcgtat ctctgctgaa ccaggaacga aagcttatgg ttcattatcg attgctgttc
    96181 aatattatat ggaagccagt ttagcattca tcgtaccaaa aacagtattt gtccctcagc
    96241 caaatgtaga ttcagcgatt ttgaaactga caagaagaga tataccggca gtcgaagtga
    96301 cagacgaaaa agcatttttc cgattgacaa aagcggcatt tcagcaaaga agaaaaacgc
    96361 tttggaataa cttgcagcat tcgtatggca aagatgacca gacaaaagca tggttagcaa
    96421 aaagcttgga aacagctgga atcgatccaa aacgtcgtgg ggagactttg tcattacaag
    96481 aattcgccgc attaagcaat gcgatgtcag aaaatatgca gtaaaaaaag aggttgtgac
    96541 agaagttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
    96601 ctcatgtttt tcgtttgttt gggcttatta ccgcagatgc aagcttttga acaccgttta
    96661 agaaagaggt tgtgggcctg ccgtttaact ctaagtcaca aggaacaatt tcaaaaaaca
    96721 gcttctcatg tttttgagtt agttggtctt attacttgat gcaagctttt gaccactatt
    96781 atgccgaaca aggagatgtg acacaaattt tgtcacatct cctatttttg tttcttgatc
    96841 aaaaaattct tatacttgat actcacactc attttgtatg gatttagaaa agaatatgtg
    96901 tcttgacagt tattagtaag tgagttatac taagtcgaca tttatgtgtc aagacatatt
    96961 aggagagaga aaaaatgaga aaaaattcag ttcgaaaact aacgatttct gctttattga
    97021 ttgggatggg ggtcattatc ccaatggtca tgcctaaaat catgattcca ccagcttcct
    97081 ttactttggc gtctcatgtg ccattattta ttgcgatgtt ttttacacca ggagttgcag
    97141 tggctgttgc gctaggtacg acatttggtt tctttttaac aacgccggtt atcatcgcat
    97201 tgcgagcatt gtcacatctt gtgtttgcgt tgattggggc ttggtacttg caaaagcatc
    97261 cgagtatcgt tttaaaaaat ggacagttta cttttgctaa ttggcgtttc caagggttca
    97321 attttgtgat cggtgtgatt cattctttag cagaaatgct agtagtcagc atcttttact
    97381 ttattggtaa tatgcctgaa acatattatt ctcaaggata tttttataca gtattcatct
    97441 tgatggggat tggtgggctt atccacagtt tagttgatta taatattgct tattttatag
    97501 caggaacact cagcaaatat tttgatatcc ccgtatttac ggcagcaaaa aaagaaaatc
    97561 ggaaagagtc gattcaaggg tacgcgaaga aaacgacata ttagacatga tctctcaaaa
    97621 aaaaaactct catctagtaa tggataataa atcctctaag atgagagttt tttgtcctaa
    97681 aaagagctgc accaacatct tctggcacag ctctaacagg gggcaattta tggaagtttg
    97741 atgacttgtt ctgtttcttt atcaaagaag tggcctttgc tgatgttgaa ggcaaggtcg
    97801 actatttcgc ctggtttatg gaaatctctt gcatctactt ttgagatgaa ctcagtagaa
    97861 ccagtttttg tgtaaagcat cgtttcagct ccaagtaatt cggaaacgac aacttctgat
    97921 ttcactacgg cttcaggtga agcttcaagg gctacttgtt cactgtggat gtcttctgga
    97981 cgaataccga agataacttc ttttccttca taaccttttt caactaatac tttgtttttt
    98041 ccttcaggaa tacgaagctt aagcccatgt ccatctgtga taacaccatt tttcaatgtc
    98101 acattaaaga agttcatggc aggagatcca ataaagccgg caacgaacat attgacgggt
    98161 gtatcataaa cttctttcgg tgtaccgatt tgttggataa atccgtcttt catgataacg
    98221 attcggtcag ccatagtcat tgcttctgtc tgatcgtgag taacatagat ggtcgttgtt
    98281 tcaagacgct ggtgaagttt ggcaatctca gcacgcatgg ctacacgtaa tttagcatct
    98341 aaatttgata atggctcatc cattaagaag acttttgcat cccgaacgat tgcccgtccc
    98401 aatgccacac gttggcgttg tccaccagat aaagcagctg gttttcgttt caagtattct
    98461 gtcagaccta agatctctgc tgcattgtcc acacgttttt tgatgtctgc tttatcatat
    98521 ttacgtaatt tcaagccaaa agccatatta tcaaaaacag tcatatgagg atacaatgcg
    98581 tagttttgga aaaccatggc aatatcacgg tctttaggtg ccacgtcatt catgactttt
    98641 ccgccgatat ttagttctcc ttcagagata tcttccagtc ccgcgatcat tcgcaaagta
    98701 gttgactttc cacaaccgga aggaccaaca aaaacgataa attcacggtc tttgattgat
    98761 agattaaaat cagttacaga ataattttct gcattgtcat attttttata tacgtttttc
    98821 agagccattt ctaccataat acgttccaca ctttcttttt tagttttatt aactatttaa
    98881 agtataaatg aaagcgttaa ctaacttcaa tgtcatgttg aacaagaatt ttttcttttt
    98941 tttgtcaatg tgctagtttt aagctttaat aaatatccat aagaaataaa aactgataca
    99001 ctggagtaaa taagtatgtt aacaatatca aaaggagtgt ctcttatgaa aatcgctttg
    99061 atcgctcatg accgaaaaaa acctatgatg gtgaaattag caacagcgta taaaacaata
    99121 ttggaaaaac atgaactttt tgctaccggg acgactgggc agaggatcac ggaagcaacc
    99181 ggcttgccga tccatcggtt taaatccgga ccgttaggcg gggatcagca aattggagcc
    99241 atgatttcgg aggatcagtt agatttggtg atttttcttc gagacccgct ggctgcacag
    99301 ccccatgaac cagatgtaac agcacttatc cgtttatctg atgtctatga gattccgtta
    99361 gctacgaata tcggaacagc tgaggtgcta ctaagaggac tggaagccgg cttcctagat
    99421 tttcgaagaa tcgtccatga aatagacaag catccacttt cattttgatg gataagcgta
    99481 tggaaaaata acgtttaatg ttcgcctata tgtagctata aaagctaaac aaactgataa
    99541 tatgcgatat aatacgaacg tattggataa tataaacaat aatattagtt tttcgtattt
    99601 gcagtaaatg tgaaaatatg ctatagtttc tgtgggaaaa gatcccaaaa ataatacaga
    99661 gtagaaaata aagcgttaag tgctaagagg atgggatgtt gcctcttgga cgaaggacgt
    99721 aatgttcgcg gttttatttt cgcattcgct gcatgatacg atgtagcagc tcttaaagga
    99781 gatgctaaaa atgaagaaac accggttaga cgcacgtatg attgctatta tgggcttatt
    99841 gattgcattg atggtcactt tgtcccgact ggtagcaatc gagaccccat ttattaaaat
    99901 cagtgtcaca tttattccac aagtgatcat gggcattctt tttggtcctt tttggtcagg
    99961 gataggcgca gttttagctg atttagtagg aatggccctc ttctcgaaat ctgcattctt
   100021 tattggattt acattgaatg catttattga aggagcaatc tacggattct ttttctatcg
   100081 aaaagagatc acttggaaaa atgccattct ggctacgctt agtgtcacgc tgatcatcaa
   100141 cttatttttg acaccattat ggcttgcgtt gatgtaccat gtgccattgt tcagctgggt
   100201 cgtttgggcg ccccgcctgt taaaaacagt aatatggtta ccgattcaat caattgccat
   100261 ttattacgta ggacgtagta ttccttacaa aaaaatttta agaagtttag cgatccacgc
   100321 taaataaaaa acaagcagtc ttcccatccc agaagactgc ttgtttttat tgtgctgtga
   100381 attgtgcttg atagagccgt cggtaataac catctttttg aagcagctca tcatgggtgc
   100441 ccatttcgac aatagctccc tgatccatca ctaatatctg gtccgcattt ttgattgtgg
   100501 acaggcgatg ggcaatcaca aaactggttt ttccttccat cattgctaag aaggcttgtt
   100561 ggattttttt ctctgtcaat gtatcgactg agctggtagc ttcatctaag atcaacatag
   100621 ggggattact gatcatcgtt cgagcaatcg tcaacagttg gcgttgtcca tctgatatct
   100681 ttaacccttg accgccaatc ttagtatcta atttttccgg caaacgttct acaaattcat
   100741 acatataaga ggcttttagg gcatcattga tttctgcgtc acttgcttcc ggattcccaa
   100801 aacgaagatt ttctcgcaat gtactgtcaa atagccaagt atcttgtaac accattccaa
   100861 aacttttacg taatgtatca cgttggatag agcggatgtc atggtcatcg actgtaattg
   100921 ttccttttgt tgtgtcataa aaacgcatca gcaaattaac aagtgtggat tttccggccc
   100981 ctgtttttcc tactatagca atggtttgac ctggataagc agtaaaattg aaatcttcga
   101041 tcaaacgctg cttggggtca taagaaaaag aaacatgatc aaaagagact tttcctttga
   101101 tatttttggg ttccaaggca taggcatctg gcaaatcagg cttttcttct ggctgatcga
   101161 tcaaatcaaa tgctcgttct agaccagaaa aagcggtttg cagttgtgtc gtgattccag
   101221 aaagttcgat aaatggttta gaaaactgac ttgagtagat cgtaaagctg gaaatcacac
   101281 caacagtgat tgacgaacca ctcttcaatg ccaataggcc gccaactaaa ccaatcgata
   101341 aataagcaag gtgatctaca aaacgagaag acgggtttgt caaggaagaa gaaaattgtg
   101401 cccgttgtcc tttttcatat agatcagcat ttagcagttc aaattgctgt tgactagctt
   101461 cttcttgctg aaaagctttc acgattttct ggttaccgat tcgttcggtc acaaagcccg
   101521 agatatcccc aataatcctt tgctgtctat cgaaatttgt ttgtgaagca cgagcaacga
   101581 tccaactcac aaggaaaata attggtgtag aacaaagaac gaccaatgtc agaagtggac
   101641 ttagacgcat cataaaaata aacgaaagaa tgatgattgc cactccagaa aataattgat
   101701 tgaaggccgc agaaattgca atggaaatat tgtccatatc atttgtaaat cgactgacga
   101761 tatttccgtg ggagttttga tcataataac gaagtggcaa tcgattcaga tgagcaaatg
   101821 catcttttct taattttgca acggataagt aagctaaccg attgcttaac acttggatga
   101881 gccattgact gactacgttg attaaaacga taccaccgaa tagaataagt aaatggaaca
   101941 gtcgattgaa gcggacctgt ccttctccaa tcatttgatc gatactttga ccaatttgta
   102001 acgtcatcac aactgttgca atcccattga tgattcctag tatcacagct agccaaatgt
   102061 cttttgcata ggcagtaagg taaggaatga atcggcggag cgtctgcaag gataattttt
   102121 ttactttcat aactattctt cctcctgtga tgcgacgatt tcctgatatt ctgctgattg
   102181 agcaagtaat tcttcatggg taccctgagc agcaatctta ccttgatcca tcactaagat
   102241 atgatgtgct tcttgaatag agcggacacg ttgggagata ataatcactg tactgccaag
   102301 ataagtctgt agcgccttac gcaaatccaa gtctgtttga taatccaacg cacttaaaga
   102361 gtcatctaaa atcagtaatg ctggcttggc aatcaaagct cttgcaatcg tcagtcgctg
   102421 tttttgtccg ccagagaaat ttttcccatt ttccatgact tttgtatcta aaccatttgg
   102481 aagctgacgg acaaattctt ctgcctgagc gattttcaga gcttcccaac attctttttc
   102541 tgtggcgtct ttcttacccc attgaaggtt ttcacgaata gtcccggtaa atagtacaga
   102601 agtttgggga acaagagcaa tttgctgacg gagatgatcc agctgccaag aacgaacatc
   102661 cattccgtcc attataactg taccggaact gacatcatag aaacgcggaa tcaattgtgt
   102721 caaagtcgtt ttcccgcttc cggttggacc aatgattcca aggactgtat ttcgcttcag
   102781 ctgaaatgta atattttgga gagaaagacc agaatcagga gtataacgga agtcaacatg
   102841 gtcaaaagcg ataattgctt cagaagaaaa atcgggagta gtactgacct cagcttcaag
   102901 aatcgaattt tctgtgtcta gcacctcttt cacccgattt cctgaagctt cggcacgtgt
   102961 gaagatcacg acgaggtttg aaacaacgat cagtgccaaa agcatttgat tcatataatt
   103021 gatcaaagca agaacttgtc cttgttgcaa gctaccaata tttactttga tcccgccaac
   103081 tgtcagcaaa cagatgatcc cgacattcat gatcaaggtt gtagcaggtg ataatagggc
   103141 agagatattg gaaacacgta aataattgtt cgctagatca tttgtgcttt tatcgaactt
   103201 ctctgtttct ttttttgtct gcgcaaatgc gcggatgaca cgaacaccgc taagattctg
   103261 actaacgtgt tcattgagct gatctagttt ttcttgcact ttttggtaga gaggaacggt
   103321 ttttttgatg ataaaaaata aaatgattga gaaaataggc aataatgcta agaaaaacag
   103381 acctacttcc cagtcgatca caaaagccat gaccactgac ccgatactta aaaacggtgc
   103441 tctgatcaat aaacgaatga ccattgctaa agctagctga agctgattga tgtcgtttgt
   103501 cattcgggtg atcaaggtat ctgttccaaa atgattcaac tcgttgtgag agaaagtatt
   103561 gatttttttg attagctgat tacgtaactc tgtaccaaat ccttgtgaag ctactgaagc
   103621 atagtattgg caaatgatag cacaaatcag accaatcaca gacatagcga ccatccataa
   103681 gcccatttca atgatttttc ctcggtcatt catttttagt ccattatcga tcaaggaagc
   103741 catcattaaa ggcaggacaa gttcgaagac agcttcaaga aatttgaaga acgggcccaa
   103801 aatgatttgc ttacgatagt cttttgcata acgtaataac tgaatcatat gattcctcct
   103861 attatttatt tgtccacaat tgaaaaaaca agtactacca cattgtagct taggaaattt
   103921 agaatgaaaa gagaacaagc cttttcataa atgaaagttt tattatgaag aagctttaga
   103981 aattgaatag actatttttt ttaaaaaaaa gaaaatgaga aaacatgcca gtctcttcat
   104041 tcataggaac gtctatttta cttaattttt ctataaatga attctgcata aaaaatatat
   104101 caagtaaatg tttatagatg aaactctatt tacttctttt ttaatttgtt cttgcaatct
   104161 tttttgctac gtaatttttg taaaaaagtg ggttgagaga aatctaattg attttcaaag
   104221 aaggagtatg aaaatgagaa aaatatttat tcgtttattg tgggtactat cttagaggaa
   104281 taaataatag tggagaaaac tatggtttgg cgcagattct atcaggtttg atggaacgaa
   104341 gacaacctcc cgacatcaag tcaaaagcta cttgagattt tgatctgaaa ataaaaaaag
   104401 aaaacaaggt aggaacatca atgtttctac cttgttttcg taatcaatgg gaaaaaacta
   104461 agagtggtgt acgtaatcaa taaaaaggat ggtgacaaca gtacctcttt actctctgaa
   104521 agcatcataa ctcgtttttc ctcattatgc caataaattg ttgttatacc acatataagg
   104581 atttgtaata gctaaaaaca gacaggaaag ttgaaatctg tgaatcttat tttgtatgga
   104641 aggatttata gaacgcaaaa atcaagataa agatgaataa aaatcctgtt tcggctatat
   104701 attgcgtatc tacgccaata aaacgagttt gttctgtcat agccatataa agcaaaggca
   104761 aaaatacaac tacagcgaga taagatccga tgatttctcg aaaagttttc caggaagtat
   104821 gtttcctgtt tttatattta tccgatttca ttgaatagtc atagcctcct tattctttca
   104881 tatgttgaaa ttccagtata aaaactggaa ggaactgtac gttttttaga ataaaaatta
   104941 taagggataa gtaggctgct gtctataata agatatattt ttcaattctt tattaaagat
   105001 tttattgaca aaatgaatcc gttttctatg atggaggagg ggtgaataag ttgttgaaga
   105061 aggaaatttt atgttactta gagaaccaga cagccttttt cgatttagaa catatgaacg
   105121 aggtctttac agcacaaagg ttagctgatc attttggggt aaaaagaaac acagtcagtc
   105181 attatttgaa ccagttaaat gatgagcaac tactagtcaa aatcaattct cgaccagtga
   105241 tttatttcca caaagaagct tttgaaaaac aattttttaa gttacgcacg aatttttatt
   105301 tttctatcga tgatctcaaa gaggaacagc cttttttcgc gcaaccaaaa gacttattct
   105361 cttttatgat cggatacgac gccagtttga aagaaagtat cgaacaaatc aaaacagctc
   105421 tttactatcc agatggcgga ttaccacttt tgattacagg cgaaagtggc acaggtaaaa
   105481 gttatttagt taatttggtc tatcagtatt gtctgcttca tgatctgcta gaagattctg
   105541 caccattcat cacttttaat tgtgcacaat atgcagataa tcctgaactt ttgactagca
   105601 atttgtttgg acatgttaag ggagcgtaca ctggtgcaga agaaaataaa aaaggggctt
   105661 ttgaagcagc agatggtggg atcttgtttt tagacgaggt acatcgctta acaccagaag
   105721 gtcaggaaaa actgttcacc tatcttgatc aaggtgtgat ttatcgtatg ggagaaccta
   105781 atttgtcacg ccggatcaaa acacgtctct tttttgctac gactgaagag attactagtc
   105841 atttcttaac aactttcatt cgaagaatac ctatccgaat cgaactacct gcacttaatc
   105901 agcgtagtcg taatgaacgg ctagaactga tttactcttt ttttctcgaa gaacaaagaa
   105961 aaatggggag ccctttgtca gtcagtggac cagctttgtc acttttaaca agtcagaaat
   106021 tatccggaaa cataggagag ctgaaaaata tcgtcaaagt ttcagcagca aaagcatatg
   106081 ctgaacagcg tgaacagcct gaaatccatg tcacgatcca tcatttgcaa aaagaactac
   106141 ttgcgcagcc tattactaaa aatacagcac agcaaggaat ctatattacg caagaccaga
   106201 ccttagagca attaatggag aagcaacagc ccgaacagca gcgaatcgtt aaaagttttg
   106261 agcaaatcct aattgatttc aagaaaaata actgtctgct atattcttgc gaaggaaaat
   106321 taaagcaaga agtgattcaa ttatttgatt ttttgctgtt tgagaccagt agacaagaaa
   106381 aacacgagct gctggtttat tggacacaat ctatccgaga gacctttcga caaatggaaa
   106441 ctgcctatca aatcaaattc gatggaaaca gtgtctatgc cttgagttac tatctctatc
   106501 agcgaagcac tatcaaatgg ttaccggaag aacatgatat tatccaacta atcaaagaat
   106561 tagaacaaca agtttctcag tcttatccat ctagttatca ctatgtccaa cgaattctgg
   106621 aattaagtaa gatcaaatta gatattgaag acacaagtat ggatcggatt ttactgacga
   106681 tctatctaaa aaaagcgaaa tggtcaaaag aaaaacgact gccaaaagcg atgattgctg
   106741 ctcacggtta tgcaactgct agcagtatgg caaatgtagt aaatcgttta ttacaagagg
   106801 atctatttga atcatttgat atgccactag atgtcacacc acagcagatt gcttcagaaa
   106861 tccttgatta ttgcgaaaac agcgatgttt caaatggatt gatcatatta gtagacatgg
   106921 gatctctgaa agaaatccat cagtttttta aaaaacagtt atccgtaccg cttttgattt
   106981 tgaataatgt gacgacacct ttagcaatca cagtagggga atgcctgcaa aaaaatgtat
   107041 cattagaaga gatagcagaa gagactgttc gacagatcca gccagaatgg cgactgctct
   107101 atccagagga aaacaaaccc aaagcactca ttactacttg cttcactgga atcggtacgg
   107161 cggtccactt aagtgaactg ctagaaaaaa gcttacctac ggcttgtcag ttgaaaatca
   107221 tcccatatga atatcagcaa ttaaaagata agaaaaacag tgacccatta ttttccatat
   107281 acgaagtcgt aggaatgatt ggcaccacag atcctgatat caccaatatc gactatcttt
   107341 cattagaaga cctcatctcg ggggaaaaga tgtctgtact agctgaatgg ttggacacta
   107401 ctatgaatgc ttcagagaag gaaatattca atcagcgcat cattcgtaat ttttcattgg
   107461 aaaaagtact ggattccgtg actattctag atacagagaa ggtgatggga gaaatcgatc
   107521 attttatgag agaattagag gtgcagttga atcgaagcat cagcaatccc aaaaaattag
   107581 cgttgtatgt ccatgtgagc tgtctcattg aacgattgat ccgacaaatg ccaatcgaaa
   107641 cctatcaagg attagagaaa tggaagcagt gtcaaaaaga agggttatct gctattaaat
   107701 ctgcgtttag tgtcattgaa gagcattata gtgtcatgat ccccaattct gagattgcct
   107761 atatctatga tattttgagt gaagatacag agtttctctc gatagaagaa gaattttgaa
   107821 cataagcaag ttgttgccac taattttggc atgcaacttg cttttttagt atgtgtaagt
   107881 gaaaagagga gatgaaaaag tggatagaga aattatctta gcttcccatg gaaaatttgc
   107941 ttcagggata ttagattcat tagagctgat ctatgggaaa aatcattcta ttactgtatt
   108001 cgattgctat actgatgaga attttgattt agcggaaaca gtaaggcagc tttttatgag
   108061 gtacaaagat aaagaaatca ttgttctgac ggatcttttt ggcggaagtg tcaacaacga
   108121 attcttacaa tatatcaatc gggatcatgt ctatctcgtt gccggattga atttgccact
   108181 attgattgag ctagtatctc ggttggacgc tgaagtagag acaactgttt tgatccagca
   108241 ggtgctaact gattcaaaaa aaatgatcca gttttgtaac gagcggctca atcagaaaat
   108301 agaagaagac gaattttagg aggaagaaaa atgattgtat tgacaagagt cgaccatcgg
   108361 ttattacacg gacaagtagc tttttcatgg acacaaacaa taggcgcaga ctgtattttg
   108421 attgctaatg atgatgtacc tacgaatgag atacgaaaga caacgatcaa attagccaag
   108481 ccacaaggag tcaaactagt gatcaaatca attgatgact cgattgccgc gttgaaaagc
   108541 ggggtgacag ataaatataa gttattcatc gtagtggaat cgatcgagga tgcatataag
   108601 ctggcagaag cgtatccaga aatcaaaacc atcaacttag gagggatcaa ggcaaaacct
   108661 ggtactcgca gcatcggaaa agcggtaaat attttaccag aagaggagga acgcttgaaa
   108721 cgattaattg acaaacaaat agaagtagaa atccgtcaag taccagcaga taaaaaagtg
   108781 gatgtaagag aagttttgta agaaaggaag aacctcaatg attgtacaag cagtcttatt
   108841 aggaattgtt gcatttgtcg cacaatcaga atatgcacta gggacatcgt tgctttctcg
   108901 tccgattgtg acggggctgt ttacaggaat cgtgttaggt gatgtaaaaa ctgggatcat
   108961 tatgggcgca actttagagt tagcatttat cggatcgttc tcagtaggtg cttcgattcc
   109021 acctgatgtt gtgaccggtg ggattttagg gacggctttt gctattacag ctggagccgg
   109081 tacagaaacc gcgcttttac taggtttgcc gattgctacg ttgacattga tattgaaaaa
   109141 tatttactta gggcttttga ttccaattat gaaccataaa gcagacaatt atgcagaaga
   109201 aggaaattat aaaggagtcg agcatatgca cttgcttgcc ggtatcggtc tttccttgat
   109261 gcttggtgtt gtggtaatgg tttcttatat ggtgggaagc aatacgatcg gaaatctatt
   109321 aaatatgatt cctgacttcg ttcaaaaggg gttgtcagta gctacagggt tgattccagc
   109381 tcttggtttt gcgatgcttg ctcgtttgct gatcaacaaa caagttgcac catatttttt
   109441 cttaggtttt gctatagctt cttacttaga gattcctgtg accggagtag ctatttttgg
   109501 tgcgatttta gcagttgtgg tagtcaatat tatgaatgtc cgtcaactac cacttcaaac
   109561 aactgcaggg gaggtagaag aagatgaaga attctgagat caaaaacagc aaaataacga
   109621 aaaaagagct aaatcacgta ttttggcgtt cttttcaaat ggagttttct tggaattatg
   109681 aacgtcagat gaatatggct tatgtttatg caatgattcc gattttgaaa aagttatatc
   109741 agtcgaaaga agaaatggca gcagctttga aacggcatct agagtttttt aacacgaccc
   109801 cacatatcgt tacactgatt ttaggaatca cggctgcaat ggaggaagaa aataaggatg
   109861 atcctgaatt tgatgtgact gcgatcgata gtatcaaaac atctttgatg ggacctcttg
   109921 cagggttagg agattctttc ttttggggaa ctttgcgatt gattgccaca ggtgtgggta
   109981 cctctttagc catgcaagga aatattttag gccctatctt gttcattttg attttcaata
   110041 ttccgcacat tctgtttcgc tatctagcaa caggttgggg atatcgctta ggaacgggtt
   110101 ttttgaaaaa gatccaggca aacggtatga tggaaagttt gacattaggc gcgtctatta
   110161 ttggactgat ggtcgttgga gcaatgactg ccacgatgat caccatcaat attcctttaa
   110221 aaatcggttc aggagaaaat gctgtgactg ttcaaagtat ttttgatgat atcgttccaa
   110281 atatcttatg tcttggagca tttggcatag tgttttatct attgaagaaa gaagtgaaac
   110341 cattaacgat cttgatcgga ctagctttct ttggaatttt tggttcgtgg atagggattt
   110401 tttaaaaaag gagcagagaa aatgtttacg atgcaagatt atatctatga agaaaaggaa
   110461 gtactaagta cgatcttgaa aaagaatgat ttttcgacaa gagaacatat gaaaaaaaca
   110521 gtgaatttac tgatcttagc aacgggttca tcttataacg cttgtttagc agcgaagcca
   110581 gcattggaat cttatggtga tcttactgtc gatattcaag aaccttttta ttttttcaat
   110641 tatggaaaac tttctccttc aatcgatact gtgattgctg tgtcacaaag cgggaaaagt
   110701 gcttctactg ttgaagcagt aaaaatgata caaaaacaag ggttacctgt tgttgctatc
   110761 acaaatgacg tgcaaagtcc acttgcttta gaagctgcac aaattattga tttaggtgcc
   110821 ggggtagaat cagtaggctt cgttacaaaa ggctattctg cgactgtttt acagttacta
   110881 ctcttaggga taggaattgg aataagtaaa aataaaatca gcaaaaaaat agaacaagac
   110941 tacatgcaac agttgcgcaa aataatcaac catttaccag cgattattca aaaaacggaa
   111001 gaattttttg atggatatca aagtttattt cgtttagcac agcgtttcgt caccattgga
   111061 tatggtccaa actggggaac agctaaagaa gcagagacga aattgactga aacaatccgc
   111121 gttccgtcac aaggatttga gttagaagct tatatgcacg gcccatacct ggaggcagat
   111181 gcttcgcatt tgctattctt tattgaaggg gaaagtgtaa ataaggaacg ttcgcaaaag
   111241 ttgcaaagat atatgagtcg gtacgttgga gagactctca ccattaccac aaaaaaagca
   111301 agaaacgaga aaacacttgg attagcaata gaatgtgatg aatatctgtc tgtattagcg
   111361 cttgtcgttc cgtttcaact attcgcttat aaaaccgcag tagctaaagg tattgacttg
   111421 aacaaaaaga tttttgaaga ctttgataca gtcttgaaaa gtaaattata agaggtggaa
   111481 atcatgctga aattcaatga agaagaacaa atcaaagcaa taaaaggcgc actagcttta
   111541 cgtccgcaag tagaagaaat catcgataaa ctttatgcgg aaaaatttga cgctgtctat
   111601 tatctaggaa tcggtggaac gtatgcttcc tcgatgcaag ctgtcactta tatgaatggg
   111661 aaaagcaact tgcctgtctt tgtacagcat gctgcagaat attacactac tggaaacaaa
   111721 agattaacaa aagattctat cgttattttg tcctctgtta ccggtacaac tcaagaagta
   111781 gtgaaagcag tagaacaaat caagaaagta ggagcgacac tgattggatt tatcgataaa
   111841 gcgaacagta agttgtctca gctatgcgat tttgtggtca cttatccagc tcctggaaca
   111901 gaacaaatca aattttttat ggcagcagat cgattgatgt atttgaatgg agaattcgaa
   111961 gcttattcag aatactatga acagctcgaa cgttatttac caacagggtt agtggaagca
   112021 gagaaaaaag cagatgcatt tggcttatca tttgcagaaa aacatcgacg tgatagtatg
   112081 cattatttta ttggagcagg gaatcaatgg ggagctgttt attcatatgc catgtgttac
   112141 tgggaagaac aaagctggct tccatcgaaa tctatccatg ctgccgagtt tcttcacggg
   112201 acgttagaga tcgtagaaga aacaacccca gtcacattat ttttaggcga agatgaacag
   112261 agagtgttat ctgaacgtgt ggctaaactt ttgccaaaaa tttgcagcaa ttatacattg
   112321 atagacacaa aagactatcc agtagaaggt atcagtgaaa aatatcgcgg acgtgttttg
   112381 tctttcttat tgatgcatgt agtgacacaa cgcatcgatg ctcatgtgga gcagttgaac
   112441 tgccatccat tagagatacg ccgctactat cgtcagtttg attattagga tagactggat
   112501 aaaaagaata taaaaaataa agagagcagc gataactgct ccctttattt ttttaaaata
   112561 ttggcatcaa ttacgttata aaacacattt tttgcattat ctacagttca acttataatc
   112621 tgtttaatgg gacatgtggg tgccaaccat tttttgttat gcaatagtac tatttagttg
   112681 ttgcatggat ccgtagtaaa agtccaagta caacgagttg cgccagttta catagcgttt
   112741 tgtatccaga atgttgacta aaacaaaagt tcctatgtca accgtcaaca gagacaatct
   112801 gtccgttgca ggacctgaca gatttagaaa tgttcaatct gtctcttatg tattttaaat
   112861 caatactcac taggagaagt ttcctaacct cctaatagta atgctggtag taacattgtc
   112921 aaaactgctt cctcttagga acaagcaaag cataacgagt gtaaaaataa aatgtagact
   112981 attgtaacat atcattgatt atttcaagta aaaacgataa aagaagtaaa caaacactta
   113041 aaaacaatgg aaattagtcc caactttaag aaatgtatac tataatggaa attatcaatt
   113101 atttatttgg aggaatatcc atgaaacaaa aaatcgcagt tcttggtcct ggatcttggg
   113161 gcactgcttt agcacaaaca ttggcagaaa acgggcatga tgtccgtatt tggggaaatg
   113221 tacctgaaca gattgatgaa atcaataagt atcatacaaa tcagcatttt ttacctgatt
   113281 tgacgatccc agaatcgatt atcggatata aagaattggc agaagcagtg gataacgcag
   113341 atgctatttt atttgttgtt ccaacaaaag cgattcgttc agtcgcacag gaacttattg
   113401 gtaaaatgaa tactaagcca atcattatac atgcaagtaa aggactagaa caagatacgc
   113461 ataaacgaat ctcagaagtt ttggctgaag aaattccaga agaaaaaaga caggcaattg
   113521 ttgtattatc tggtcctagc catgctgagg aagtggcagt acatgatatc acaacaatta
   113581 cagctgcaag tattgatcaa aaagcagcag cctatgtaca aaaactgttc atgaatgatt
   113641 atttcaggat ttatacaaat aatgatgtga ttggcgtaga aactggtgca gcactgaaaa
   113701 atatcattgc aattggtgcc ggcgctatcc atggattagg attcggagat gatgcaaaag
   113761 cagcaatcat gactcgagga ttggcagaaa tcagccgtct tggtgtcgcg atgggtgcca
   113821 atcctctgac atttatcggt ttgagtggtg tgggggactt gatcgttact tgtacaagtg
   113881 ttcattctcg caattggcgt gccggtaact tgttaggaca gggacataag ctggaagaag
   113941 ttctagaaaa catgggaatg gtcgttgagg gtgtggcaac gacaaaagca gcagtagagc
   114001 tagctcaaca gctaggtgtt gagatgccaa ttactcaaac aatctataat gttttgtata
   114061 atggagaaga tatcaaaaaa gcagcaaaag aaatcatgtt acgtgacgga aaaatggaga
   114121 atgaattttc tcttcgttaa atagatgatc aatagttaat cgt
//
LOCUS       DASHFF010000002        99753 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.002, whole genome shotgun sequence.
ACCESSION   DASHFF010000002 DASHFF010000000
VERSION     DASHFF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 99753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.002"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(55..498)
                     /locus_tag="U2I75_000105"
     CDS             complement(55..498)
                     /locus_tag="U2I75_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN1695272.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            632..1003
                     /locus_tag="U2I75_000106"
     CDS             632..1003
                     /locus_tag="U2I75_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1695273.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(1172..1954)
                     /locus_tag="U2I75_000107"
     CDS             complement(1172..1954)
                     /locus_tag="U2I75_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN1695274.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(1951..2922)
                     /locus_tag="U2I75_000108"
     CDS             complement(1951..2922)
                     /locus_tag="U2I75_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN1695275.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            3014..4408
                     /locus_tag="U2I75_000109"
     CDS             3014..4408
                     /locus_tag="U2I75_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1695276.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            complement(4600..5955)
                     /locus_tag="U2I75_000110"
     CDS             complement(4600..5955)
                     /locus_tag="U2I75_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN1695277.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(5985..6620)
                     /locus_tag="U2I75_000111"
     CDS             complement(5985..6620)
                     /locus_tag="U2I75_000111"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN1695278.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            complement(6627..8003)
                     /locus_tag="U2I75_000112"
     CDS             complement(6627..8003)
                     /locus_tag="U2I75_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN1695279.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            complement(7996..9777)
                     /locus_tag="U2I75_000113"
     CDS             complement(7996..9777)
                     /locus_tag="U2I75_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN1695280.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            complement(9799..10110)
                     /locus_tag="U2I75_000114"
     CDS             complement(9799..10110)
                     /locus_tag="U2I75_000114"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN1695281.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            complement(10100..11086)
                     /locus_tag="U2I75_000115"
     CDS             complement(10100..11086)
                     /locus_tag="U2I75_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN1695282.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            complement(11101..11685)
                     /locus_tag="U2I75_000116"
     CDS             complement(11101..11685)
                     /locus_tag="U2I75_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN1695283.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            complement(11706..12176)
                     /locus_tag="U2I75_000117"
     CDS             complement(11706..12176)
                     /locus_tag="U2I75_000117"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN1695284.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            complement(12195..14198)
                     /locus_tag="U2I75_000118"
     CDS             complement(12195..14198)
                     /locus_tag="U2I75_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN1695285.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            complement(14185..14517)
                     /locus_tag="U2I75_000119"
     CDS             complement(14185..14517)
                     /locus_tag="U2I75_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695286.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14854..15573
                     /locus_tag="U2I75_000120"
     CDS             14854..15573
                     /locus_tag="U2I75_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN1695287.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            complement(15872..17770)
                     /locus_tag="U2I75_000121"
     CDS             complement(15872..17770)
                     /locus_tag="U2I75_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEN1695288.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(17933..19195)
                     /locus_tag="U2I75_000122"
     CDS             complement(17933..19195)
                     /locus_tag="U2I75_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695289.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(19188..20087)
                     /locus_tag="U2I75_000123"
     CDS             complement(19188..20087)
                     /locus_tag="U2I75_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695290.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(20232..22010)
                     /gene="uvrC"
                     /locus_tag="U2I75_000124"
     CDS             complement(20232..22010)
                     /gene="uvrC"
                     /locus_tag="U2I75_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN1695291.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(22192..22506)
                     /gene="trxA"
                     /locus_tag="U2I75_000125"
     CDS             complement(22192..22506)
                     /gene="trxA"
                     /locus_tag="U2I75_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN1695292.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(22593..24953)
                     /locus_tag="U2I75_000126"
     CDS             complement(22593..24953)
                     /locus_tag="U2I75_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN1695293.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(25085..25615)
                     /locus_tag="U2I75_000127"
     CDS             complement(25085..25615)
                     /locus_tag="U2I75_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEN1695294.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            complement(25673..26110)
                     /gene="zapA"
                     /locus_tag="U2I75_000128"
     CDS             complement(25673..26110)
                     /gene="zapA"
                     /locus_tag="U2I75_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN1695295.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            26312..27229
                     /locus_tag="U2I75_000129"
     CDS             26312..27229
                     /locus_tag="U2I75_000129"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN1695296.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(27485..28396)
                     /locus_tag="U2I75_000130"
     CDS             complement(27485..28396)
                     /locus_tag="U2I75_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1695297.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(28799..29377)
                     /locus_tag="U2I75_000131"
     CDS             complement(28799..29377)
                     /locus_tag="U2I75_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1695298.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(29397..30065)
                     /locus_tag="U2I75_000132"
     CDS             complement(29397..30065)
                     /locus_tag="U2I75_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695299.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(30080..31150)
                     /locus_tag="U2I75_000133"
     CDS             complement(30080..31150)
                     /locus_tag="U2I75_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695300.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(31321..32685)
                     /locus_tag="U2I75_000134"
     CDS             complement(31321..32685)
                     /locus_tag="U2I75_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1695301.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(32682..34241)
                     /locus_tag="U2I75_000135"
     CDS             complement(32682..34241)
                     /locus_tag="U2I75_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1695302.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(34297..36777)
                     /locus_tag="U2I75_000136"
     CDS             complement(34297..36777)
                     /locus_tag="U2I75_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695303.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(36761..37096)
                     /locus_tag="U2I75_000137"
     CDS             complement(36761..37096)
                     /locus_tag="U2I75_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695304.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(37110..38144)
                     /locus_tag="U2I75_000138"
     CDS             complement(37110..38144)
                     /locus_tag="U2I75_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1695305.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(38234..38914)
                     /locus_tag="U2I75_000139"
     CDS             complement(38234..38914)
                     /locus_tag="U2I75_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1695306.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(39198..40301)
                     /locus_tag="U2I75_000140"
     CDS             complement(39198..40301)
                     /locus_tag="U2I75_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1695307.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(40930..41400)
                     /locus_tag="U2I75_000141"
     CDS             complement(40930..41400)
                     /locus_tag="U2I75_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN1695308.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            41730..43370
                     /locus_tag="U2I75_000142"
     CDS             41730..43370
                     /locus_tag="U2I75_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN1695309.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43524..44846
                     /locus_tag="U2I75_000143"
     CDS             43524..44846
                     /locus_tag="U2I75_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN1695310.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(44914..45348)
                     /locus_tag="U2I75_000144"
     CDS             complement(44914..45348)
                     /locus_tag="U2I75_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1695311.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(45413..46249)
                     /locus_tag="U2I75_000145"
     CDS             complement(45413..46249)
                     /locus_tag="U2I75_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN1695312.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(46991..47617)
                     /locus_tag="U2I75_000146"
     CDS             complement(46991..47617)
                     /locus_tag="U2I75_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695313.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(47912..48247)
                     /locus_tag="U2I75_000147"
     CDS             complement(47912..48247)
                     /locus_tag="U2I75_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1695314.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(48234..48518)
                     /locus_tag="U2I75_000148"
     CDS             complement(48234..48518)
                     /locus_tag="U2I75_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN1695315.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(48574..50097)
                     /locus_tag="U2I75_000149"
     CDS             complement(48574..50097)
                     /locus_tag="U2I75_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1695316.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(50090..51265)
                     /locus_tag="U2I75_000150"
     CDS             complement(50090..51265)
                     /locus_tag="U2I75_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1695317.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(51269..51454)
                     /locus_tag="U2I75_000151"
     CDS             complement(51269..51454)
                     /locus_tag="U2I75_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695318.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(51420..53114)
                     /locus_tag="U2I75_000152"
     CDS             complement(51420..53114)
                     /locus_tag="U2I75_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1695319.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(53111..53584)
                     /locus_tag="U2I75_000153"
     CDS             complement(53111..53584)
                     /locus_tag="U2I75_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN1695320.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(53653..53808)
                     /locus_tag="U2I75_000154"
     CDS             complement(53653..53808)
                     /locus_tag="U2I75_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEN1695321.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(53942..54322)
                     /locus_tag="U2I75_000155"
     CDS             complement(53942..54322)
                     /locus_tag="U2I75_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1695322.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(54326..54541)
                     /locus_tag="U2I75_000156"
     CDS             complement(54326..54541)
                     /locus_tag="U2I75_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695323.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(54544..54951)
                     /locus_tag="U2I75_000157"
     CDS             complement(54544..54951)
                     /locus_tag="U2I75_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEN1695324.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(55217..56692)
                     /locus_tag="U2I75_000158"
     CDS             complement(55217..56692)
                     /locus_tag="U2I75_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEN1695325.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(56682..57530)
                     /locus_tag="U2I75_000159"
     CDS             complement(56682..57530)
                     /locus_tag="U2I75_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEN1695326.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(57567..57929)
                     /locus_tag="U2I75_000160"
     CDS             complement(57567..57929)
                     /locus_tag="U2I75_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695327.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(57930..58088)
                     /locus_tag="U2I75_000161"
     CDS             complement(57930..58088)
                     /locus_tag="U2I75_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695328.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(58229..58853)
                     /locus_tag="U2I75_000162"
                     /pseudo
     CDS             complement(58229..58853)
                     /locus_tag="U2I75_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(58850..59161)
                     /locus_tag="U2I75_000163"
     CDS             complement(58850..59161)
                     /locus_tag="U2I75_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695329.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(59205..59498)
                     /locus_tag="U2I75_000164"
     CDS             complement(59205..59498)
                     /locus_tag="U2I75_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695330.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            59692..60339
                     /locus_tag="U2I75_000165"
     CDS             59692..60339
                     /locus_tag="U2I75_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1695331.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            60400..61545
                     /locus_tag="U2I75_000166"
     CDS             60400..61545
                     /locus_tag="U2I75_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1695332.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(61984..62802)
                     /locus_tag="U2I75_000167"
     CDS             complement(61984..62802)
                     /locus_tag="U2I75_000167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695333.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(62902..64227)
                     /locus_tag="U2I75_000168"
     CDS             complement(62902..64227)
                     /locus_tag="U2I75_000168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695334.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            complement(64396..65325)
                     /locus_tag="U2I75_000169"
     CDS             complement(64396..65325)
                     /locus_tag="U2I75_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695335.1"
                     /translation="MRSDSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTR
                     HIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSA
                     KTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHMRNYSQHGHLPVNIH
                     QQRVYFDLDEILAMPHFDFNGQLKKELDVLRKDIYNQFRDSPRISYVYTVAKFNLVIT
                     EIYFNYLKEIKPILIKLNDEKNELLLNTKFQLINSDGSSSDMLFYEFDGENYHCFNRK
                     DNSISMYADIKREVKKILKVEEQYYKEIEKRNQ"
     gene            complement(65573..65923)
                     /locus_tag="U2I75_000170"
     CDS             complement(65573..65923)
                     /locus_tag="U2I75_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695336.1"
                     /translation="MIFENESEEIGRMAEENKVVPFETLVINKEEFEQLFLDFFIQYD
                     RIYDFSSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFR
                     EFRNQIIRSSNIEN"
     gene            complement(65964..66458)
                     /locus_tag="U2I75_000171"
     CDS             complement(65964..66458)
                     /locus_tag="U2I75_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695337.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
     gene            complement(66451..66765)
                     /locus_tag="U2I75_000172"
     CDS             complement(66451..66765)
                     /locus_tag="U2I75_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695338.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(66981..68372)
                     /locus_tag="U2I75_000173"
     CDS             complement(66981..68372)
                     /locus_tag="U2I75_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1695339.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(68357..68968)
                     /gene="mobC"
                     /locus_tag="U2I75_000174"
     CDS             complement(68357..68968)
                     /gene="mobC"
                     /locus_tag="U2I75_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEN1695340.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(68972..69169)
                     /locus_tag="U2I75_000175"
     CDS             complement(68972..69169)
                     /locus_tag="U2I75_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695341.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNEIESDFKCCSLEKNVRG"
     gene            complement(69183..71876)
                     /locus_tag="U2I75_000176"
     CDS             complement(69183..71876)
                     /locus_tag="U2I75_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEN1695342.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAK
                     NSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQG
                     TKTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDV
                     RENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIID
                     SEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            complement(71863..72081)
                     /locus_tag="U2I75_000177"
     CDS             complement(71863..72081)
                     /locus_tag="U2I75_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695343.1"
                     /translation="MIENRLIDQVWCLDQNTDNLELIETICFNTIVLDLRSENDNCVT
                     HIIIDRKTAQKLSESLRKWAEGGNNENS"
     gene            complement(72157..73170)
                     /locus_tag="U2I75_000178"
     CDS             complement(72157..73170)
                     /locus_tag="U2I75_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1695344.1"
                     /translation="MEEIDKLIAQYAYSKDDVYAEKYLKYPIFLDKHKKYKKMKQSTK
                     KVYMYLKKQNEYSLENSKVDSKGRIFLIFAIEKIAEKSCLTKKTVISALRELEEYKLI
                     TIKKNGFNKKTGKNNPNFYYLLKPEFSEGDFYKKKCIEENLHLPTNQHYSPSEKNTPS
                     DKSTFDGDKSFPLPGEKFLQELNHSSKDFKDFKESQNADFIDSLSVENKKLDEILIES
                     LIEKEWLYQVWGDNIVNCLKLVGNNDYSQFYDWYKKFKYAIKSAEKEQKIQLKIIYEI
                     CNNKYFVDYVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGI
                     DKQ"
     gene            complement(73374..73655)
                     /locus_tag="U2I75_000179"
     CDS             complement(73374..73655)
                     /locus_tag="U2I75_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1695345.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
     gene            complement(73706..73912)
                     /locus_tag="U2I75_000180"
     CDS             complement(73706..73912)
                     /locus_tag="U2I75_000180"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1695346.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            74076..74807
                     /locus_tag="U2I75_000181"
     CDS             74076..74807
                     /locus_tag="U2I75_000181"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1695347.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYKKLLQTDKSDDQLILG
                     FIYICLDEKLSNRQKSLKSINYEVFNYLGVAFGLIDSEIKSTNAGNDEDLLNYLKELT
                     SFIDEIKTTTFNKKKYFQNQYDIEE"
     gene            74861..76006
                     /locus_tag="U2I75_000182"
     CDS             74861..76006
                     /locus_tag="U2I75_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1695348.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(76091..76396)
                     /locus_tag="U2I75_000183"
     CDS             complement(76091..76396)
                     /locus_tag="U2I75_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN1695349.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(76421..76852)
                     /gene="ruvX"
                     /locus_tag="U2I75_000184"
     CDS             complement(76421..76852)
                     /gene="ruvX"
                     /locus_tag="U2I75_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN1695350.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(76849..77115)
                     /locus_tag="U2I75_000185"
     CDS             complement(76849..77115)
                     /locus_tag="U2I75_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN1695351.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(77370..78095)
                     /locus_tag="U2I75_000186"
     CDS             complement(77370..78095)
                     /locus_tag="U2I75_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN1695352.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(78456..78526)
                     /locus_tag="U2I75_000187"
     tRNA            complement(78456..78526)
                     /locus_tag="U2I75_000187"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78492..78494),aa:Gly,seq:ccc)
     gene            78643..79395
                     /locus_tag="U2I75_000188"
     CDS             78643..79395
                     /locus_tag="U2I75_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1695353.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            79411..79890
                     /locus_tag="U2I75_000189"
     CDS             79411..79890
                     /locus_tag="U2I75_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1695354.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(80141..81721)
                     /locus_tag="U2I75_000190"
     CDS             complement(80141..81721)
                     /locus_tag="U2I75_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN1695355.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(81714..82457)
                     /locus_tag="U2I75_000191"
     CDS             complement(81714..82457)
                     /locus_tag="U2I75_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695356.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(82633..83022)
                     /locus_tag="U2I75_000192"
     CDS             complement(82633..83022)
                     /locus_tag="U2I75_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEN1695357.1"
                     /translation="MKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLMSMPGYK
                     NKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFAN
                     GEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(83223..83408)
                     /locus_tag="U2I75_000193"
     CDS             complement(83223..83408)
                     /locus_tag="U2I75_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HEN1695358.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFS"
     gene            complement(83656..84492)
                     /locus_tag="U2I75_000194"
     CDS             complement(83656..84492)
                     /locus_tag="U2I75_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN1695359.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(84503..85432)
                     /locus_tag="U2I75_000195"
     CDS             complement(84503..85432)
                     /locus_tag="U2I75_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN1695360.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            85624..85977
                     /locus_tag="U2I75_000196"
     CDS             85624..85977
                     /locus_tag="U2I75_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1695361.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(86051..86707)
                     /locus_tag="U2I75_000197"
     CDS             complement(86051..86707)
                     /locus_tag="U2I75_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1695362.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            86933..88867
                     /locus_tag="U2I75_000198"
     CDS             86933..88867
                     /locus_tag="U2I75_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1695363.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(88953..90176)
                     /locus_tag="U2I75_000199"
     CDS             complement(88953..90176)
                     /locus_tag="U2I75_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1695364.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(90170..92068)
                     /locus_tag="U2I75_000200"
     CDS             complement(90170..92068)
                     /locus_tag="U2I75_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN1695365.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(92062..93180)
                     /locus_tag="U2I75_000201"
     CDS             complement(92062..93180)
                     /locus_tag="U2I75_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1695366.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(93263..94096)
                     /locus_tag="U2I75_000202"
     CDS             complement(93263..94096)
                     /locus_tag="U2I75_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1695367.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(94089..95006)
                     /locus_tag="U2I75_000203"
     CDS             complement(94089..95006)
                     /locus_tag="U2I75_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1695368.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(95233..96357)
                     /locus_tag="U2I75_000204"
     CDS             complement(95233..96357)
                     /locus_tag="U2I75_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1695369.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            96540..97448
                     /locus_tag="U2I75_000205"
     CDS             96540..97448
                     /locus_tag="U2I75_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1695370.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            97589..99202
                     /locus_tag="U2I75_000206"
     CDS             97589..99202
                     /locus_tag="U2I75_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1695371.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        29636 a        20221 c        16269 g        33627 t
ORIGIN      
        1 tatgtactgt aataaataaa aaaactgaga gaaagctgat ctactcgctt tctttcagtt
       61 tttatttttt tcgatccatc tctcgactgc ccatcggtgg ctaccacgtt tcaatttcac
      121 aagcgcttct tgagggctca cccaccaaat tttattggtc ttttcagttg gttctcctat
      181 acgcttccaa gaatcagcaa caaagaaata tcctggatta taataataag ttgcacgata
      241 attcgagtaa aaatactcat cagcttctcc cagataagaa gaaatttctg ccacaattcc
      301 catttcttct atcagctcac ggacaatcgc ttcttctttc gtctcttctc cttcaatttc
      361 cccgccagga agaaaaaaag caccatttgg agcctcgatt actgcaatct gctgatctct
      421 ttctatgaca atatatgctg cataacgggt ttgataatca gcaaattgct ttttttcacc
      481 aaaaattggt ttatccatga atagtttcca ccttaacagt tttattttct atccatttta
      541 catgagaaat ttctcatgaa caagcacaaa tcaactattt ccttacttct ttttgttatg
      601 attgagtaaa aggaaaagga gagatgaatc tatgaaaatg gctcacacat gtgttcgagt
      661 gaaagatctt gaagcatcaa tcgaatttta tcaaaaggca tttggttttg aagaaagccg
      721 ccgccgtgat tttccagaaa acaaatttac acttgtttat ttgacacttc ctggggatga
      781 ctatgaatta gagttgactt acaactatga tcatggtgcg tatgatctag gagacggcta
      841 tggtcatatc gctatagcag ctgatgatct agaaaaatta cacgagcaac ataaagctgc
      901 cggtcttgag attactgact taaaaggatt acctggcact gcgccttctt actactttgt
      961 cattgaccct gatggttata aaatcgaagt catccgagga tagttagtat ggggcatttt
     1021 actcttgatt gaataaaaaa caaacaccaa taattaaaat gagacgcaaa cttttcatta
     1081 aattgaaaaa gtttgcgtct ttttttaaca accattgtag ctataaggtt gccactctcc
     1141 accaatttat ttttaattca tttgaaagaa atcaaataaa cgaggtacct ttagaaaaca
     1201 aaactgcttt tcctcctatt atcacacgat cttcttttaa ttcacattct agatccccgc
     1261 cccttgatga catttgataa gctgacaatt ttgttttatt cattctttct gcccaataag
     1321 gaataagatt agaatgtgct gatccacata ctggatcttc attgattgtt aattttggaa
     1381 agaaggttct agaaacaaag tctttttgca cactttctgc tgtcacgatg actcctattc
     1441 ctaattcaaa atttttgata gctgtaaaat ctggctgcaa tttagccacc gttttttcat
     1501 cttctaatac gaaaaataaa tcacgtgcta agtaggcttc tttaatctta gccccaatcg
     1561 cttcttcata ttcagacaat atcggcactt ttttcggcat aatactagga aaatccatgt
     1621 aataataatc tttaattctt gtcacaaata gattaccgct ttgagtagaa aatttaatcg
     1681 tttcatcagg tattttgtaa tagttaaata aaacaaatgc tgttgctaat gtcgcgtgac
     1741 cacaaagatc gatttctcga tctggcgtaa accatctaag ctcaaattct tggtttttct
     1801 tcactgtaaa tgctgtttca gataaattgt tttcaatggc aattttttgc atcgtacctt
     1861 ctggcagcca ttcttctagc acataaaccg ctgctggatt tcctttgaac acctcatctg
     1921 caaatgcatc aactacgtaa taatctaagt tcattttaaa gcccctttct ctaatttgtt
     1981 ataaatatat tctgcacaac aaagatcttc aatagctaga ccaactgcgt cgaaaatcgt
     2041 cggcctcgat tctcctttat aaaatgtttt tttctcagaa actaattcgc ctaatgtacc
     2101 aacaatacaa gattcctcta aatctccttc tgccatggct ataagcaggt ccccgctttc
     2161 tgttaaggca gctgagtaat catctacata gatttgagaa gtttccatca acgatgtact
     2221 tatctctctt gtatcaggag taaatgtgcc cacagcattg atatgtgcgt cttcccttac
     2281 ccactctttt tgaagaaatg cctctttact agaggtcaac gtacaaatga tttccgctgg
     2341 acgtactgca tcttctactg atattgcatt tataaacgtt attttaggat actgtttttt
     2401 agctttagcg ataaaagtat ttcgtctttc ttctaaaata tcgtaaacaa acactgtagt
     2461 gatgggacga atggtagaaa tcgcagtcaa atgcgacaat gcttgttgac cagctccaat
     2521 taaagcaagt gtttctgcat gcggatttgc taagtaatct gttgccagcg cagatacagc
     2581 agctgttcga atccaggtga ctgcatttgc gtttatcaat gctttgggta gtccgttttg
     2641 agcatcaaac aagaaaatct ctcctaaatg agaaggtaaa tgatactgat gattatccgg
     2701 aaaagcggta atgacttttg tgccaaaata tctgttctcc cccaaataag caggcatcat
     2761 cgcaaacata tttttgcctt ttccgtcagg taaaatttcc accaatcgag gattttggct
     2821 gattttacca ctttcataat caaaaaaaca ttttttcatt acttgaatcg cttcttcaaa
     2881 actaaaagaa gctactactt cctcaaaatt aataattttc atagacaatt ccctcctcac
     2941 aaaagattaa cttctaaaat caatacaatc aatggtaaaa ttgtatggta tacaatttta
     3001 aggagtggag aaaatgccta ttaattctta tgacaattac ccgttaactt ggcgaccaga
     3061 caaacaaaaa ttgaagcgtc cttattatcg atcgttgatt caacaattag aacaagatat
     3121 tttaaagaat aaattacaga aaaacactca gttaccctcc caaagagagc tagctgatta
     3181 tttagattta aattttacta ccgttggcaa ggcttataaa tatggaattg agaaaggttt
     3241 gctgtatacg aatattggaa gtggcacatt tatttctccc aatgtctttg agtccatcac
     3301 catctctacc aatgaagtaa ccgaacatat cattgatttt ggtctggtta gttcttttga
     3361 agaatgtaat aaattcgttg tcccgtttat tcaatcaact agtaataacc caaatcttgt
     3421 tggtttgctt aattaccaag atcccaaagg cacaaatttt caattgacta ctgcagcaaa
     3481 ttggttaaaa actcaaggag tcattccgtc cttagaatct ctatctattg tttccggtgt
     3541 tcaaaatgga ttggctatca cactttctgc acttttctct ccaggtgata gaatagcaac
     3601 agaccgatat acatacgcta atttcattga attggctcag ttatatcatt tagagatcgt
     3661 tccaattgat tttgacgagc aaggaatgcg acctgatata ttgattcagg aatgtagaaa
     3721 aaagaagatt catggtgtct ttcttatgcc ttcttgtaat aatcctatag ggtttcaaat
     3781 ctctcacaaa agacgagagc aattagccga agtgattgaa aaagaaggtc tatgggttat
     3841 tgaagatgat atccattcat ttttaacaac ttactatcaa catacgatca ttcctccttt
     3901 ccaaaattta ttacctaacc aaacaatcta cttagctgga atgacaaaat ttatttgttc
     3961 tggattgcga gttgcttatc ttgtagttcc agtacatgtc caaagcaaga tagaacaagc
     4021 aatttttaat ataaatgtga aaacttctgg attagatgtg gaagtcatca ctcaaatcct
     4081 ccattcaaat gaagcaaata ggatcttaga aactaaatta agtcttacaa aaagagccaa
     4141 tcagcttttt gattctcttt tcaaagtaga aaaaccaacg aatcgtgtcc cttactatcg
     4201 aattcttcca gttgatcctg agattgaccc taagataatt gagaatgact ttcttgctaa
     4261 cggtatccga ctttatcatt ccagacgctt tactacacgt gcgcaaccag atgcattcgt
     4321 tcgaatttcc ctatcctcta ataaccttga aattttagct tctggactaa gtatcgtaaa
     4381 agaaaaaata aaaaaatatg aattataacc tattgataaa taaaggaatc aaatcttatt
     4441 gattcctttc aataaaaaag aggcactacc cgaggttcaa caacgggtag tgcctcaaaa
     4501 aataaacgaa cattttgcac ttaaagtatc caagaagcaa gaaaaattat tttattttct
     4561 tattttacta aatcactagg tttctgtcag aagatgaaat tatccaatca tgacactttc
     4621 ttctggatat ttgtattttg attgtgttct ttgtgctttt gtcatcagag agaagaccac
     4681 agtcaataca ccaactcgtc caatgtacat aagcgctata atgatcaatt tccctattgc
     4741 agtcaagtct ggcgtcaatc ccatcgtaag acctactgta ccaaacgcag agaagacctc
     4801 aaaagcaata tattcgatgc cagaagtttt aggtatcgtt tcagtcaccg ataaaatcat
     4861 gatggcgact acacacaacg tcaacgtaac aaaaaataaa gtaagtgccc gcaacactgc
     4921 gccttgtcgg atggtacggc caaatgcttc cgctctcgta cgtcctttca acatcgaacg
     4981 catctggatc agcaacacac ctaacgtagt tgttttcaat ccgccggccg tcgacccaga
     5041 tgttccgccg atgtacatca aaaacatcgt taaaatcaga cctgcatggc tcatatggag
     5101 gtaatcaact gaataatatc cagccgttct aggtgtcaca ctcataaaaa aggtgtttgc
     5161 caatcgttga aacatattcc cttccactag cgttgcccca ttgcgctctg tgaaaaagaa
     5221 cacaacgaat ccgcctaata aaagggtacc tgtaacggtt aatgcgattt ttgaatgaat
     5281 cgtcatcttc tttttctgat gatattccag taaatcacgc catacgatga agcctaaccc
     5341 accagaaatg atcaaagcag ataccaccat gatcaaataa acattttgct ggtgattggc
     5401 taaactgtct cctaacaaat caaaccctgc attacaaaaa ctagagattg catggaacaa
     5461 actgtaccag atacctttca accagccaaa ttcaggcaca aatacaaaag ataaagcaac
     5521 cgtaccaatc aattggataa caaaggccaa ttttaggatg taaagcatga gcttcatcac
     5581 acctgatact tcttctagat tcagcgcttc ttttaatata atacgtgtgc tgagactgac
     5641 tttcttcttt atcaatgcaa agcatagaat cggaaccatc ataaaaccaa gaccgccgat
     5701 ctcaatcaaa agcatgatca aaaattgacc agcactgttc caatgctctg ctgtattcaa
     5761 tgttgtcaat cccgttacac agacagcaga agttgcagtg aaaagcgcat cgataaaatt
     5821 tgtcccatgc ccactattac taaaaaatgg tagggtaagt aggccgccgc caactaaaat
     5881 caggagaaaa aatcctaatg cgagaagctg aacagccggc aaacgtttct ttatttgttt
     5941 gctttgttta atcatcgttt tcctctttta aaaaaagata ccacttagtc agtggtaccc
     6001 atatttttta ctttgatcaa ccttgttact tctgcgcgct cgttttcttc cagcttcata
     6061 cgaatataat agatcgtttc ttctaattga gggatcgtca tatattctaa tgcattgact
     6121 cgccgacgtg tcttttcgat ttcactcgcc atcagttgac aagttttttc gatttctgtc
     6181 agttccaaaa gttttggaag cagggtagta aaccgatcga ttgaccgatc cagttcggca
     6241 ctggaattca aatacccgta ttccaaaggt gtgtctgtca gctgttcatc atattcaaaa
     6301 ttcatcacag gtacttttac actcatgata tttttgtcta cgatagacaa atcaacgttt
     6361 tccgccggca aaacaaataa ttcttcgatg aaaggttccg tgatcgaagc atttgccaat
     6421 acaaaatcag acattgcttc tgtcatagac tcttccattt cttgacgcag ctggttgttc
     6481 tttcgaatca acaggatgaa ttgacgcatc agttcatctt gcttatcttt taataattta
     6541 tgccctcttg tggcagttgt caactgtttt ttcaatcgag tcaattccat gcgggtcgga
     6601 ttcacattca atcgtgccat cagaactcac ttcccttctg gcagatattt gtccagcatg
     6661 tcgtctttga ttcgttttaa ttcggttctt ggcaacatcg atagcagttc ccaccctaga
     6721 tcaagtgttt cagtaatcga acggttcgtg tagaatcctt gattgacata ttctttttca
     6781 aaccgctcag caaacttcgc atagatcttg tctacatcag ataaagcaga ttcacctaaa
     6841 actacggcta attcttttgc ctgttttcct tgtgcatagg ctgcaaaaag ctgattcatc
     6901 gtagctgcat ggtcttctct tgtctttccg ggtccagttc ccttatcttt taaccgcgaa
     6961 agagacggga gtacatcaat tggtggctga atcccactct tgtaaagctc tctagtcaag
     7021 atgatctgtc cttctgtaat ataaccagtc aagtcaggga ttggatgagt tttatcgtct
     7081 tctggcattg ttagaatcgg aatttgagta accgatcctt ttagtccgcg gattcttcct
     7141 gctcgttcga ataaagtagc taagttcgtg taaagatacc caggatatcc tcttcttcct
     7201 ggaacttctc gccgtgctgc agaaatctct cgcaatgcct ctgcatagtt ggtcatgtct
     7261 gtcatgataa ccaaaacatg cattcccttt tcataagcaa gatattctgc agcagtcaaa
     7321 gccatacgag gagtagcgat acgttcgatt gcagggtcgt ttgctaaatt cataaacatg
     7381 acagaacggt cgattgcgcc cgtttgacga aaatcttcca tgaaaaattc agcttcttca
     7441 aaagtgattc caatagcagc aaagactacg gcgaaatcat ctgatgaatc gagtaccgtt
     7501 gcttgtcgcg cgatctgagc agctaattct ttatgaggaa gaccagagcc agaaaatact
     7561 ggtaattttt gcccacgaac taatgtattc aaatgatcga ttgcagagat acctgtttgg
     7621 ataaactcat ctggataatc ccgagctact ggattgatta cttctccgtt aatatccaaa
     7681 tgcttttcag gcaggatctc tggtccgtta tctttcggtc gtcccaaacc atcgaatact
     7741 cgaccgatca tgtcttccga tacacctagt tctaaaggat ggcctaaaaa tcgtacagaa
     7801 gagtcacgta aattgatacc acttgttcct tcgaatattt ggaccattgc tttatcttct
     7861 tggacttcca atacttgtcc acgacgaatc tcgccatttt gcatacggac ttcgattaat
     7921 tcttcatatt tgacaccagc aactttttca accgccatca atggaccgac gacttcaccg
     7981 atcgtacgat attctttaat cattgctcat gcctccttct gaaacgatcg tttgcatcgt
     8041 ttgcttgatt tcaccaataa tttgatctag tttttctaat tctgtttccg ggatatattt
     8101 gcttcgtccg atcctttcac gaatcgatac ggtaccgtcc atgatctcac ttaaataagc
     8161 acctaacgac aacgcacggc gtccttcttc accaaaggtc aagatcatct tcaacatttt
     8221 gaattgtttc tctcttgaag tgaatgtatc gacatcatca aaagcatttt gctgcaaata
     8281 atcttcacga atggattttg ccacttctaa tgtcagacgg tcattatctg aaagggaatc
     8341 aatccctacc aaacgcacga tttcattcag ctgctgttct tcctgcaaaa tacgcatgcc
     8401 ctcggtgacc atggatgccc agtcagcttg aaggatatga tccatatact tccctacttc
     8461 tgttgaataa agagaatagc tttgtatcca atcgatcgaa ggaaaatgtc ttttttgggc
     8521 taaactggaa tcaagacccc agaatacttt tacgacccgc aatgtatttt gtgtgactgg
     8581 ttccgaaata tcccctccag acggggatac agcactgatt gcggttatac ttccttcgcg
     8641 accttctgat cctaatgcaa tgactcttcc agatcgttca tagtattccg caagccgtga
     8701 accaagataa gctggataac cttcatcacc aggcatttct tccaaacgtc cgctcatctc
     8761 acgaagtgct tctgcccatc gtgaagtgga gtctgccatg atagcaacgt cataacccat
     8821 gtcacggaaa tattccgcaa ttgtgattcc cgtatagata gaagcttctc gagctgctac
     8881 tggcatgttt gatgtattag caatcaatac cgtacgttcc atcaacgatt ctcctgtatt
     8941 agggtcaatc aattcaggga attcatttaa aacatccgtc atctcatttc cacgctcgcc
     9001 acatcccaca tagacaacca tgtccacatc ggcccatttt gcaatttgat gctgaacgac
     9061 tgttttccct gcaccaaaag gaccaggtac agctgctgct cctccttttg tcactgggaa
     9121 gaatgtatca atcactcgct gtccagtgat catgggtgca tccgggttta atttttcttt
     9181 gattggccga ctacgtctta ctggccattt ttgaagcatc gtcaaatttt tgtcaccatt
     9241 ttcggtttca atgacacata ctgtttcatc aatagtgaat gaaccttttt cgattgtttt
     9301 gatcgttcct tttatcccat ttggcaccat gattttatgt tttataactt ttgtttcttc
     9361 tacgatcccg ataacatctc cagcactcac cgtttctccg gcttctactg ttggttcgaa
     9421 ccaccatttt ttctcatggt ccaatgcggg caattgaact ccgcgcccta agaaattact
     9481 ttgtgtgatt tccataaagg tatctaatgg ccgttggatt ccgtcaaaca tttgtgaaat
     9541 gatccctggt cctaattcaa cagataaagc ttcaccagta gagcgaaccg gttcgcctgg
     9601 accaattcca gaagtctctt catatacttg aatagacgcc acatcgccac gcatctcgat
     9661 aatttctccg ataactccta gatctcccac tagacacatg tcttggatac tggcatcaga
     9721 catgttctca gccattacaa gaggtccgga gacttttatg atttttccaa tttgcaaaat
     9781 ttctatctcc ttttcatact ataagatatt ttgacctact gctttttcta cgtttttttg
     9841 aatttcttcc agccctatcc ctaatgatcc ttgatgattg ggaatcaata cgatagcagg
     9901 tatcatttgt tccttgtaac gttcgatcgt ttctgggctc aatgctgcac attgctctgt
     9961 gacataaatc acgccatact cattctttgc catttcttca attgcctgac gaacggtttg
    10021 cttggttgta ccataacgaa catcaaagcc aaacaatttg aatggcaaaa cagaatcttt
    10081 gtcgccaact acaccgatct tatgcgtcat agacttgtct cacccgcttt ctgattgttt
    10141 ctggttcgaa atggcttctt ttgccgataa ccagtaaacg aaggtttttg ctttctacct
    10201 cttttgcgtt caaaaaggct agtagtggca gtggtccgaa agcctgggtt tgtgcttctt
    10261 gatacaatga acttagataa tcatctttca actgttcaag ctttacgaga tcaagatttc
    10321 cctcacggat gacagggcgc agaaaatcag cataatctgt ctctaataga aaattcgtgt
    10381 aatcttccat atctttatct acaaagttca aaaatacttc tttttcgata cttcccgagc
    10441 tggaaagtac cgtcgtcatg aacccatatg ttcttttctg caatattcct cgagccatcg
    10501 ttgtgatatt ggtcaagtca ataaaagcaa tgacttctgt cagcaattct tgatatccta
    10561 gcttatcggc aagccgtcgc tgctctgtca aaaactgtcg atcataaatc acgtcaattc
    10621 cctgaagaat ggtagactct tcaaaataat cacgtacttc tcgtatactg tccataatgc
    10681 tttcagggag ttcatttgaa acttgcgtat gaatggcatc cttcaaggtt tctaatgaat
    10741 aaaatccatc gtcgatatac aaatggtcca gattttgatt tgtcatctcg gcttttgtca
    10801 aaaccttcag attatggaaa gtatagcgca tcgtatagat ccaaacgatt tcaggctccg
    10861 gtgctgattc ctttaaccag cgaaatagac ggctctgctc ctttaaaaga ttctgttcga
    10921 agtcatattc atatccgtca taaatatatg gactgtaaat cgtggatttc aatatttcac
    10981 caagtgcttc gacagaattt gtttgaatca tccgttcaaa tgtctcattg gacaaaagtt
    11041 caagctctct tccgcgaatc aagggattta gttcgtggta ttccatacac tgatccctcc
    11101 ttattcgaat aattgattgg caatctcaaa actcatcgtt ccttggatat cttgtacaag
    11161 gctgctgaaa agaaaattat actgaactcc ttgatcgttt atcagaaaac ccgcttgatc
    11221 agcaacagtt tcatcactta aaaccatcat aaagggaagt tcattgttcc attcatccag
    11281 caatgtctga gaaagataag ctgctgattt ttctccagca atgaatgtta ctttgcctgt
    11341 caaaggtaat gaatacaatg aatttttgat aaactggatt tgttcagatt catcccaatt
    11401 ctccatttca gctattgcat ctgcaaacaa tcgtcgtaaa aattcctgct tcgcattcaa
    11461 attttcttgt cgtacttcta cttgttggcg attcctaagc tgacggtatt ttttttcaag
    11521 taattcgatt tgtttttctt tttgtttttg atgttctgtt tcgatttgcg tacgtttttg
    11581 ttcaaagttt tgatcgatct tcattcgctc ttcttgctct aacgccgctc tttcttgctc
    11641 tgctgcttca ttcatctgag agatgatttt ttcaatggcg ttcatctgct tttcctcctt
    11701 tatgcttagg cattcaatac taataggaag gaaataacga aacctaaaat cgcatatgtt
    11761 tcaaccatcg cagcaaaaat gattcctttt gtagcatgtt caggtttttt cgctaaaatt
    11821 tggatacctg ctgctgcaac tttcccttgg gcgatacctg aaaacaaacc tgtaaaagca
    11881 atcggcagag aagcgcctag aaagttcaat ccttgtacaa ctgacatatc atttcctaag
    11941 ttgataaaaa tcaaaaaggc gataacgaat ccatacaacc cttgcgtacc tggaagcaat
    12001 tgcagaatca aagcctgtcc gaatttttcg ggttgacttg ttgttaacgc tgctgctgcc
    12061 tcacctgtca ttccaacacc tttagctgaa ccaatccctg aaaaaatcgt cgctgttgcc
    12121 atcgctaata ctgcaaatac cattccacca ttttgactaa ttaagtaatc catcattgta
    12181 taaaaatcct ccaattattt attgtttttt ttcttatgat tgatgttgac gtatttttcc
    12241 gccgttttca gcgggtcaaa ggcacgtccg cctcctgtat aaaattttcc aaaaaattcg
    12301 acatactgca atcgtgcacc atggacatag gcacttaaca gtgtcaaaaa catattcagt
    12361 gcgtgtaaag cgatgatcaa aaggatccct acactgaatc gagcggctgg cggcatgaaa
    12421 gcaactagca tattgaatgc cgcgccaata cttccgcctg aaatacctag tgccatcaaa
    12481 cgtgtataac tgactaaatc accgatataa ccagtcaatc cgtaaagatt atacgctcct
    12541 ttggcagcac ccttgacttt agacggcgat tggataatcg gcacgattaa gatacataaa
    12601 gcagcaatga tcgatagccc cgcgccaaga taaacaaatg ctgtattttt tatcaacatg
    12661 acaccaatca atgcaataac gatcccaatc aggatccctt gccaggcaaa accatcactg
    12721 acagcactca agtaatcttt cctcctgata ttttcctttg ctgccacgaa taatccaacc
    12781 aagatctgta tcagaccgaa aatcacggat aaaatcaaaa tcgtattgac gtcatccgtc
    12841 gtagatagga ttgggaaagg caagcggata ccaaatagtt cttttggtaa tgcctgtccg
    12901 aaaaatgaac tgtagatcaa tccccagatg atcgatggta ccgagagaat ctcaaagaat
    12961 tttacgaaac gtttcatccc gcggggtaaa acaaatagtt tttgagctag tattgtccca
    13021 atgagcatca gcaatccgta accaacatct gcgaccatca tcccgaagaa gacaagataa
    13081 aatggtgtca tccaaggcgt tggatctact tcttcatatt ttggcagact gtacatttca
    13141 gtcagcaatt caaaaggttc gataagcgga tggtttttca atttagtagg gacttcctgc
    13201 tgaatctctt ctgagtttgg tgtttcgaaa ctaacgtata tatcttcttc tgatacagtt
    13261 tcgtttaaca catgcatcaa attctgtttt tcttctgcat ctacccaacc ttgcaaaacg
    13321 atcagataag tggcatggat aaaccgttct tttacttttt cccgttctgc aacggcaagt
    13381 gtgacttctt ctacccattc aaattcttgg atatagccgc tgcactgacc taacgctgtc
    13441 gttatctttt tttgctgctc gtgaacaatg gataattgct gtttggtttc ttgcaatttt
    13501 tctttaggat tttgttcata aggatacgtt tctttattga acccgtattt cccagcaatt
    13561 tcttctatag cagcaactga tttttttaaa gcaacatacg aaagataaac ttgttttggc
    13621 gtataatcta tttcttccat ataaacgtca tcaagcttac ttagttcttc cttgaaactt
    13681 tcaaaatttg ctgcactcac agtccccatc tcaaaaatgg tcgtcttact tgcatacata
    13741 cttggagcaa tatccagctg ttgccaatga agaagccatt cttcctcttc ttctaaacga
    13801 tcacgttttt cagttagctg ttcccacttt tcttttaaat caaggacttc ttctaattta
    13861 ttcatgaaat tttcttcgga ataatttgct tccaagtctt gtaaggaaag ttcttgacgt
    13921 ttcaaatgga gtttcttttg acggctatcg ccgtggtgtt gtatgaattg gatagcttct
    13981 tggatgtctt tcagccgatt gcctaaagcg cttaattttg cttctttatc aataatcgtg
    14041 gtatctggaa aatatttttc tacccactgg ttgtttgttc tattttgata cagatcccgg
    14101 atttcaacat tttggattcc ttgtactgct tgtaaaatcc tctcctgatt tcttttatct
    14161 gaaatgaggg tcactttttc catcttagtg acggccataa gtttctttca ccctttcaat
    14221 gattgcaccg attgcttgac tctgatgctt ttgatattgg tctttcaaag ttgcttgcac
    14281 cttgtctgct tctgctttca atttcatttt ttctttttct aattcttgct tttcgaattg
    14341 ttctttttct tctaccaact gtttcagctg ttgccgactg ttcgtttgga ttatctccaa
    14401 ttcttgctct ttttcttctg cataggtctt caactgctgc cgtagggttc gagcacgctt
    14461 ctcattttct tcttctgctt ttcggatttg ttccaaagct tctaacactt gcttcacttc
    14521 ctttcgattt aaaaaagagg tacatgcttt cttttttcat tgaaagtatg tacctctaaa
    14581 gataacttag tataggcagg ccccttcaat aaaatgtcct tctcacccct tgaagaacta
    14641 gatccttgaa gcttacgccc aaccttttta tttctgctta ctttttatta taacaaatta
    14701 agcaatattt tcaactatcg tcatgaattt cacacgaaaa ttctctttac agatagtaac
    14761 acagcaagaa cagataagta taggggcaag tcttacatag tttaaatttt tttaaatcct
    14821 tgttacaatt agtctacttt agggggaata aaaatgaaac caatcatcgg cattgccggg
    14881 aatcaactga ttcgagcgac agatactttc caaggaaatc aagtaagcta tacaccgcag
    14941 ggatttgtgg atgcagtttt agatgctcaa ggattacctc ttattctgcc agtcatgtct
    15001 ccagattcag cgcctcaact aattgagaaa atcgacaaac tcattcttgc aggcggtcag
    15061 gatgttagtc cgcaacttta catggaagac cctcatccaa aactgacgga aacaaatatt
    15121 caacgagacc aattcgaaca ggcattgatc ttggaagcat tgaaacaaag aaaaccgatt
    15181 tttgcagtct gccgaggact tcaactattg aatgtcgtgt tggagggaac actttatcaa
    15241 gatctttcat tatatccaaa atggtccgta aaacacgaac aacagcccac tgccccgcag
    15301 tttgcaactc acgaagtcaa aatcgtttcg gacagtttat taagtgacct tctccctgat
    15361 tcatactttg tgaattccta tcatcatcag gcagtcaaag atctttctcc tttgttaaaa
    15421 gccatcgcct tttccaacga tggactcatt gaagcagtcc aaagtaaaga taatatgcac
    15481 aaaatccttg gtgtccaatg gcatccggaa ctttctcatc gtgtagattc atctgaacag
    15541 cgtttattcg atttctttgt gaatgaattt taatcgtttt tctgattagg tcgtgagcct
    15601 gccgttcaac tctgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgga
    15661 tattgtgact tgtgaccgag gagttggcag gagaacaccg tttaaggaag aggtcgtgaa
    15721 atcagcgccc tgacctgagt attagagaaa taaacagaaa aatagcttct aatattttac
    15781 cgttttttga gctaattccg aaggagactg cgacacgcct tttgttgcag tcgcttttta
    15841 gtctattgag caatgttggc tagcattttt ctcaatcgtc ctttcgataa tccaacagcc
    15901 tttggagttg agtaatctgt tgctgtaatt ccattccttt tgtcgtattc cgattaagtt
    15961 tgcgtgggag ctgctgctcg atcacttttt ttacattcgt ttgatcagat ttttttgcaa
    16021 attgttgttc aataggcaag aacggataat gtgtcacgat ttggaaaccg taagaattgc
    16081 atagtaatga gtagccagca atcccggttg ttttttgata agctttggat agacctccgt
    16141 caataacaaa tgcttttccg tttgctttga tcggtgactc tccttttttt acttttactg
    16201 gcgtgtgtcc attgatgatt gctgaacctt cttcttttgc atcgaattct gccaaaattt
    16261 tttcactgaa ccactcttca tctcgcaagt gataataggg attgcttttt tcgacgtgcg
    16321 tatgaggctc tgtgagaaaa tagcgctcaa aagttgccat tttactcttg ccgaataaag
    16381 gcgaaaatgg cccgctccac gcataccaaa tcaaatcggt cgccaaatct tcatttgcag
    16441 tcaaatcttt tgcgctttca gttatctgtt tttcaaaaaa tcccagcagt tcttttccgc
    16501 gataagattg gtctaattca aatgctaaga gctgcccctt ttcatctaca ggtatacagc
    16561 catggaacag tacatgctga ttgttcgtga gatacattcc ccctttttcg atcaaaaacg
    16621 tcatgtgttt ttgcattctt aacgagtgct ggaatgaata catcaaacta tcgatcactt
    16681 tttgttcctc ttccaagaat tttttaggat catctggctg gatcgtttga aaacaagtcc
    16741 ctttcaactc atagagctga ccttctaaaa cgatcgttga ttcctcatag ttgatttttt
    16801 ccaacaattt acgatcgtcc attttaaatt caggtcgtcg attcaacaac tgatgttctg
    16861 atttgaattg gaggatagaa agtgcttgat ggatcttttc taattcttct tgctctcttt
    16921 cagatagatc tttcctgttt ttaggggtaa atactggatt cttctgatag gtttgatggg
    16981 caaaagaaaa taatggacgt aaatttaatc catattcttt ctctaattca aataaatagt
    17041 tgtagcgtgc ggcaatccgt aaaagcgtca ataaacatgc ctcagagccg caatacgcac
    17101 caatccacaa aatatcatga ttcccccact ggatatctac agaggaaaaa tccatgattc
    17161 gatccattac tttggctgct tgagttcctc gatcaaaaat atcacccacg atatgcaaat
    17221 gatcgatgac agatttttgg atcgtctgcg caagttttgt taaaaatttt tcttcttcct
    17281 tcatcaagac aagttgttca atgatttttt ttacatagtc ttttttcccg ttgatacgat
    17341 catcgatata caagagttcc tcaatgatat agctatattc tttcggcaat ccttttcgaa
    17401 cttttgatcg agtgtatttg actgtcgtaa accgtaaaag atcgatcaaa cgatggatca
    17461 gctgggattt atcttgtttt ttgtaccaat cagtcgtcaa cacatattct ggataagccg
    17521 taaaaatagt gaattggctg atctccgttt ccgtcatttg ttcagagaaa aggtctttga
    17581 tcttttcttt aagatttcct gaaccgatac gcagaatatg atcaaaagca ggaaattcgc
    17641 catgtatatc gctcaaaaat aactctgttc cttttggaag ctgcaaaatt gcttcgagat
    17701 tgatgatttc cgtgatgaca tcttccgttg tagggaactt ctctgaaagt aaatctaaat
    17761 agcggtccat taaaacgcct ccaaaaattg gtatactcaa cattataaag ttgttatgct
    17821 ctattttcaa tgaatatata tatccaatat aataaaaaaa tagaaatagg tctatacaaa
    17881 aaagaagctg ggaacaataa atgtccagct tccttctata tttttctata ttttatgctt
    17941 tttttcgttc atttctcata attgaaatcg attgtttcaa tgaactccat aatccgcctt
    18001 cgctatacac gagtacattc gatttgtaca tgtttgcaga caagatcatc aataagaatg
    18061 ttacggctac aagtaccaac aaggaaatca atgctccacc cataccgact gtctcatttg
    18121 ctaagcgaac cggcataata tatgaagata agaatggaat atacgaagtg atccgaataa
    18181 cgatattgtt tggatctgaa gctcctagta tcaatccaag catatatccg cctagagata
    18241 gataagtcac tggcaaaatt gcttttgctg tatcttctgc tttgttgacc aaagagccac
    18301 ataaagctgc taaaacagaa aaaatcaaaa tgccaaagat catgaataga agagaatacc
    18361 acaagaatgg tccaatcaag ttatccaatg agataccgtc taatatattt ttgaccattt
    18421 ccatatcttt gaattgactg tatcctatac caaaaataat accgtataat gccatttgcg
    18481 ttaatgcaac aagtaaaaca cctgttagtt ttccgtaaaa atgcttttgt gcagtcgtac
    18541 tggacaggat cacttccatg atccgcgtgc ctttttcaga agcgatctct tgtgcaatga
    18601 tctgcgcata cgtcaaaata ataatgaaca agacaatcgt tgctacataa cttacgacat
    18661 attggactgt cgagttatct tctccaactg tcatttttcc ttttgaatca aaattgactt
    18721 tttgtcgagt caaacttgct tgttggctca agctagctac ttcttctggt gaaatgccta
    18781 attgacttgc tcgaagcatc gattgcaagc cagtcagttg ttgttgtatc agtagttgtg
    18841 tactttgtcc taatgaattt tcgctataaa gcatcccatt tatcttatca ttctcagttg
    18901 agactactaa atacgcatcg attttttctt ccgaaagctc tttttgtgct ttttcttcag
    18961 aatcaactgc tttgaactca aaatcatctg ttttgacttg gctcatttgc tgactgattt
    19021 gctcattatc agccaatacg ccgattttat tgacatcact attttgttga gcaaagtttc
    19081 ctgctacata aatgataccc agcatgataa aagggactaa tatcatgatc aagaaagaaa
    19141 tcgacttaac attttttaaa tagacatctt tggtaataat ccaaaactta cgcatcgatt
    19201 tcacctgctt tcaatttgaa aatctcttcc aaagttggcg gttgctggtt aaacatagga
    19261 atgtagccga actgagtcgc acgagtaaag atttctttcc cagcttcagg atcttctaag
    19321 gtaatctcta acgtattgtc ttcatgaagg atcacttttt tcacgccttc gatttcttct
    19381 acttcctgtt ggcttaaacc agattccaag aataacttcg tacggccaaa tgattcacga
    19441 atctcatgta cttttccatt caaaaccatt tttccgtttc gcaacatgat caaatgatcg
    19501 cagatttttt ctacattatc catattgtgg ctagaaaaaa tgacacatga accatgtttt
    19561 ttcaattcca atattccatc ttttagcaat tcagcattta ccggatctaa cccgctgaat
    19621 ggctcatcca aaatgatcaa tttaggttca tggattaacg tggcgatcag ctggactttt
    19681 tgctgatttc ctttagacaa agattttact ttatccgtct ttttcccttt tacctgaaat
    19741 ttttccatcc aaaaatcgat ttttggttca atctcttttc ttgatttccc tctaagttct
    19801 gcaaaataaa gcagttggtc ttgaatcgtt actttcggat aaagtccccg ttcctccgga
    19861 agataaccga tgacatcata atctttctcc ccaagatgat ggccattcca taacacttct
    19921 ccttggtctt gtgtcaaaaa atccaagatc aaacgaaaag tcgttgtctt cccagccccg
    19981 ttttggccaa ttagccccat gatttttcca tcggggattt ggaaagagac atgatcgaca
    20041 gctgtgtagt tcccaaaagt tttaacaaga tccttgactt ctaacatttc ttcactcctt
    20101 cttcaagaaa tcacatatat ccttagacac cagacgtaag cgatttatta tttaccaaca
    20161 aaacgtaaac gctctttttg ttgtacataa aatgatagga cactttactt tctttgtaaa
    20221 gtgccctttc ctcattcctg atgtaattta tcaaatacgt tttgtgcgac atttttcggc
    20281 agtccgattt cttgtaattc ttctgtcgtg gctgctgtga tatttttcaa cgatttgaac
    20341 tctttaagca gcattttttt tcgttttggt cctaaccctt caatttcatc taatcgggaa
    20401 gcaaaactgt ttttactgcg tagctgccga tggaaagtga ttgcgaatcg atggacttca
    20461 tcttggattc gctggagtaa gaaaaactct ggtgaatttc gctcaagcgg tactacagcc
    20521 aaatctatac cgaatagcaa ctcatttgtt ttatgtttat cgttttttgc catgccagca
    20581 ataggaatat cgattcctaa ttgattttct aagacatctt ttgccacatc tacttgtcct
    20641 tttcctccat cgatcaagat caaatcaggt aaaggcaacc cttcgcgaat cactcgtgaa
    20701 tatctgcgat agatgacttc tctcattgat gcataatcat ccggtccgtt gactgttttt
    20761 attttatatt tccgatattc ttttcttgcc ggttttccat caatgaatac gaccatcgca
    20821 gccacaggat tcgtccccat gatattagag ttatcaaaag actcgatacg tattggggtc
    20881 gggatattca tcgcgtttcc taaacgttca actgctccga tcgtccgttc ctgttttctg
    20941 gcaatcagat caaaccgttc attcaaagca actgaggcat tcttattcgc caattcaact
    21001 aattttttct tttctcctcg ttttggttgc agcactttcg tagctaacaa agcttctaca
    21061 ctgggcttgt cgatcgtatc aggaatcaag acttctttag gaatgaaatg ctcattttcc
    21121 tgataaaact gaccgaggta agtcaagaaa tcttcttctg cttcgttata gaaaggaaac
    21181 attgaaacat ctcgttcaat tagctttcct tgtcttacga aaaacacttg gacacacatc
    21241 catcctttgt caatcgcata accaaataca tctcgatcca gtaaatcagt atttgtcatt
    21301 ttttggcgag tcatgattgt ctcaatggca cggatctggt ctcggtattc tgcagctttt
    21361 tcaaactcca tgttttcagc agctttttcc attttctgat ggatctctga ctcgattttt
    21421 tcgtgtcctc cgtttaaaaa ctttttcact tcatcaacga ttttcgtata gactttcgga
    21481 tctacgtcga aataataagg acacagacat tgtcccaaat gatagtaaag acaaggctcc
    21541 ttcgtctgac taggtttgca ttttctcaga ggaaataatc gatcgaggat cttctttgtc
    21601 tcattcgccg caccgacatc gggatatggt ccaaaataca gtgctttgtc ttttagtact
    21661 ttacgagtaa tcaccaaacg gggatatttt tcattcgtta tcttaaggaa aggataggtt
    21721 ttatcatcct ttaacataat gttgtattta ggatcatttt tctttatcag attgatctct
    21781 aagagcaatg cttcgatatt cgattctgta acgatgtatt caaagtcttc gatttcacta
    21841 acaagccgtt ccgttttcgt attatggctg ccagtaaaat atgagcggac acgatttttt
    21901 aatattttgg ctttgccgac ataaatgatc gttccatttt tatctttcat caagtaacag
    21961 ccgggctgat caggaagtaa agctaatttg tttttgattc gttcgttcat ctggtactcc
    22021 ccttcatatt caattaaagt tattatagca tgtctgtcaa aaaaacaatc cagcctgaca
    22081 acgtaattat actcaaaaaa tattaatttg aataacctta actcaaccgc aaaaaaaccg
    22141 aggacggaac tggattcagt cccaccctca gcttattttt gctgtaagtt tttataaata
    22201 ttgatcgatc atttgacgta gttgttcttt tgaatgaaca ccaacagctt tttctactac
    22261 ttgaccatct tttttcaaca ttaatgttgg aatgctcatg ataccgaatt gttgaggagt
    22321 ttctggattt tcatccacat ccattttagc aattctgaat tcttcttcat catattcttg
    22381 ttccaactga tccaaaatcg gtgcttgcat acgacatgga ccacaccaag ttgcccaaaa
    22441 atcaatcaat acaagtcctt tatctgtttc ttcattaaat gtcttatctg taattacttg
    22501 tgacatatca aagacctcca tttttaaaat ctatgattag tatagcattt ctatacttgg
    22561 atagcgaacc atctgcttac ctttagtaag ctttacttaa atttaacaat tgtcgcacca
    22621 tttccgcctt ggtttgccgg tgcaaactca aagcttttga cgctccgatg gtttttaaga
    22681 taatcagtaa ttcccttgcg aagtgcgcct gttccttttc cgtggacaat agtcacttga
    22741 ggataaccag ctagaatggc agaatccaaa tattgatcta cctcacttag cgcttcttcg
    22801 tatcgttttc ctcgaaggtc caattgattc ggcacgtgac tactagattc agatcgtaca
    22861 gcatggataa ctcgttgtgt cggctctttt tgaggagcaa caggcgtcag ctcgtcttcg
    22921 gacacattca tttttaatat accgatttct acttgccact gattcccgtt tttacgtaaa
    22981 agcgtccctc tttggccgta agtcgtaacc aacacttcgt ctcccggttt caacgttttt
    23041 tgttctttcg ccttcttcaa tactttattt ttcttcaaag tttcttcttg atgtagattt
    23101 gctaattggg tctttgcatc gatcaactga tgctctttca catttccttg cccaatctgc
    23161 tgttgcattt tacgaatatc agcaatgatt ttttctgctt tttcttccgc ctctgacacg
    23221 acttcgtttg cttttttctt tgctttttcc atttcttttt cacgttcttc aaagaaatag
    23281 ctgtaagctt ctttcaaatc atcgtgaagt tcttgtgcct cagaaacaaa atgacgcatt
    23341 tccagatatt cggtctctgc cattttccgg cgattctcta aatctgtgat catttcgttt
    23401 aaatcttggc tctcatcatt catcaactgc ttcgcttcat caataacttc tgtatcaaga
    23461 cctaatcggc tagagatttc aaacgcattg cttcgtccag gaacgccaat cagcaaacga
    23521 tacgttggac ttagtgtatc cacatcgaac tccatgctgg cattaatcgt atttgcccga
    23581 ttgtatccat aaactttcaa ttcaggataa tgcgttgttg ccatcacata agcagacttt
    23641 ttaccaagat catctaagat tgcgattgct agagcagcac cttcttgcgg atctgttcct
    23701 gcaccgagct cgtcaaataa taccagactt ttttcattga cttttgataa aatatctaca
    23761 gtgttcgtca tatgagaaga aaaggtcgat agactttgct caatcgactg ttcgtctcct
    23821 atatccgcaa atacttcctc aaaaataccg atttgacttt cttcacctgc aggaatcggt
    23881 aatcctgctt gccccatcag ttgtaacaga cctaaagtct tcaacgtgat cgttttccca
    23941 cctgtgttag gtcctgtaat gacgatggct tgatagtctt ttccgattgt gatgtcatta
    24001 gaaactactt tttcctgatc gatcagtgga tgtcttgctt gtttcaagac gacatgattg
    24061 tcttcactga ttcccggcac gatggctttt acttctttac caaatcgtgc ttttgcattc
    24121 atcagatcca ttttaccaat cacataagca ttgtggatga tttcttgacg atggggaaca
    24181 agttcagcag aaagttcact taaaatacgt tgaatctcat ttcgttcagc gatttggtat
    24241 tgacgcaaac gattattcaa atccaccact tgttttggtt caataaataa agtctgtcct
    24301 gaagcacttt gatcgtggac tacaccgcca aatacaccgc ggtattcttg tttgactggg
    24361 ataacgtagc ggtcatttcg catggtgatg atcgcatcac ttaaatactt tgcatttttc
    24421 ccacggacga tcccatctaa ctgttcacga acagcttgtt cacttcgccg aatgttctgt
    24481 cttatgctct ttaactcagg agaagcgtca tcagtcacgc gtccgtcttc atcgattgct
    24541 tcctttaaac ggcggctcaa aacagggatc gtgactaact gatctgccca catatacaaa
    24601 cgtgcaaatt cgatttcact gtcagaaaga tcatcaatga aacgattcaa ttcgcttgtc
    24661 gtagacaata ctctggaaac ttgtgcaagc tccactccat tcaagtctgc tccgatctcg
    24721 atccgtttca tatggggacg gatattttca agtttaggta tagggatccc gcctcgcaga
    24781 cgttgcactt tcaatccatc ctcagtttct tgaagccagt ttgtgatcgt ttgtttctca
    24841 ctcactggta ccaattctgc cagttcttct tttccttgtg cagtcactac aaattgtctg
    24901 accatctgtt tgactttttc aaattccaat gtttcaagga ttcggttatt cattttatca
    24961 ccttatcttt cactatgaaa aaagagctgt gccggacata cgctacgcag ccttcttaaa
    25021 taaactactc tgtacaaaaa acgaaatatt ttccagcact attttcctta attcaaaata
    25081 ttcgttacca aagctcatac atcttgttgg taagaaatgg cgttctctca acgataaaac
    25141 gagcgaaact atgctcgccg aattgatttt ggaccacatc taatggaaca aaggcaagta
    25201 actgcaaaat caaaaatata gtaacgtaag ccatgattag tgacaggatg cctcctgcaa
    25261 tccagtctac ctgtctcaag atcggcacat acgtcaatcc atggacaaaa acccccacaa
    25321 atcgggtaag cagccagcca attagcaaaa taatcagaaa agcagttcct gcataaaaag
    25381 cttcatctag gtgaaaagca aatacctgat caaaaaatgc taactttgat gttggggtga
    25441 cagctgggta tggaatgtat aattccaaat gacttgctaa tggtttgtaa aaatgctggg
    25501 cagcaataaa tgacaataca tatccaatcg cgaatatgat ttgcaaagca aacccgcgac
    25561 gtgctccaga aaaaaacgca attagcaata aaaataaaat cagcaaactc agcatgctac
    25621 gttcctttct cttctaactt ttttaaattt ttcttatttt gataaaagga tcttaattag
    25681 aagatttttg tttaatttgt ccttttcgtt tctcgtttaa aatctgttgt gcttccacat
    25741 gatttttaat ggtatgatct gcttgaccat tttctttcaa tacttcccga gcttcgttct
    25801 cgattgcttc aatgcgtttc acacgatttt caagctctgt aaatttgatc atttttttct
    25861 ttaattctgc tgtttcttct tccagctcca agattctttc ttgtttcttc aattgatccg
    25921 aaagcgcatt gacagccatc aagattgctg cttgttcatt atccatttgt ggtgataatt
    25981 gtttcaattc tgctaactgt tcattgatca gcttcgttac gatatccata tgatgcttgg
    26041 tttcccgtcc gataatagta tatgtttggt tggcaatcac tgctttgtag cgtgtttttt
    26101 cgtgcgccat acgaatcatt tcctcctatt tgatgaaagt tttatttagt gtaccatgaa
    26161 gttggttctt tcaccgttct ccacaaatat ttttcacata cttaactatt atatgataaa
    26221 ctttaaaaaa atgctatgtc tcgcaatctc ttagaaaacg aaaagtcgtg agttgcggta
    26281 tagttttatg tataaaaacg aggagaattt tatgagcaac gtcattatca aagcaacaaa
    26341 cgaccaactt agtcaaatga aagcatacta tcgctcttat ttgttaaata aacagattcc
    26401 ttatactagc tttgctgcaa aaaaagacgg gacgaccatt accgcttata catccggaaa
    26461 agtgatgttt caaggtacga atgcagcaat agaagctgat aaatgggggg aggcacagga
    26521 taaacaagcc aagccagcaa aaacagcctc ttctttgcca aaaaatatca gtcaattatc
    26581 cgttttaggt agtgatgagg tcggaaatgg cagctatttt ggtccgctaa cagtatgtgc
    26641 tgcttatgtg aaacgtgatc agcttgcaga attacgtaaa cttggtgtta gagattcaaa
    26701 agagttaaaa gatagccaaa tcatccagct tgctgacatc ttaaaaaaaa ccatccccta
    26761 taaactgctt gtcttatctc cagaaaaata caatgagatc caaccaaaat acaatgccgt
    26821 tcggatgaaa gtcgctttgc acaatcaagc gatccatttg ctgctaaaaa aaatcgagcc
    26881 agaaaaacca gaagcgattt tgatcgatca gtttacgccg gaagcaaatt ttaaaaaata
    26941 tgcgcgttta gaaaaaaatc aattacaaga aaaactgtac ttcgttacta aaggagaaca
    27001 atatcattta gctgttgctg ctgcttctat catcagtcgt gccagctttt tggaagagtt
    27061 acacaaagca tcagctgaag ctggaatcac ccttccatca ggagctggaa cgaaatctga
    27121 ccaagtagcg gcaaaactat tggagaaagg cggcatgcct atgcttgaaa aatatgccaa
    27181 acttcatttt gccaatacag aaaaagcttt gaagctgcta aaaaaataat ggaactgtga
    27241 caaaagtcat gtcacagtcc cttgttccga ataaacggtg ttctcatgcc aactcctcgg
    27301 tcacaagtca caatagtcat caaacatgag aagctgtttt ttgaaattgc tccttgtgac
    27361 gcggagttaa acggcaggct cacaacctct tccttaaacg gtgttcaaaa gcttgcatct
    27421 gcgataataa gcccggccaa acgaaaaata tgaggagcta tttttgtttg gctgggctta
    27481 tttattactg aaaataagtg gtcaatgctt gtgtcaaact tgcagcaatc tgctgctgat
    27541 aactgtctgt tactaattcc tttaaatcac tatcattatt catataaccc atctcgatta
    27601 aaatagaagg ttgaagattt tctcgaagaa cttgatagtt cccagtggag attcctcttg
    27661 aatccagagg cagatcagtg aaggagtcag caattgtctg tgctaatgtt tcctcttgtc
    27721 catagtaata atatgtcgtg attccagttg cttcattcgc atattccgta gagtctgcgt
    27781 gtaaactgat aaagacatct gccttttttt cttcactgta gtcgcatata tctccaagct
    27841 gtactaattc atctgtagta tgggtcaaat aaaccgttgc tccagcatcc tctagcgcct
    27901 tttttacttt tatggctgta cttaatgtta tatctttttc ttctgtttgt tcatcattgc
    27961 ttaatgcccc agtatcattt ccgccatgcc caggatctaa tacaatcact gcatcagata
    28021 gctcttccgg ttttgtttgt atattctttg tgactttggt tccatcttcg agggctaaat
    28081 atccagtata gccatcattt gtcttgatct gataagcatt ttttgtttca gaaagaaact
    28141 ccgccagctc tccgccgttt atagttgcaa gtggggtagt tagcgagtca tctgtatata
    28201 gtgtacggtc attttttccg attttgatta atgtctcttg ttctgtatca tcaaatgatt
    28261 cctttgtctt tgtaaccgta ctgttcgtac tgctgaccgt ttgtccaact atcttttttt
    28321 gaccaaaaac atctatttga aaacagataa taatgataat gattgcaaat acaaaagctc
    28381 caattatttt tttcattctt gactctcacc tcgtagatcc agactaaggg tcaaaactta
    28441 aaatcagctt aagtacgcaa aaaaggggct gtaaaagata taaccgtaga aaataacatt
    28501 actgctaaaa tgattttttt catttttggc atatcctgaa atctcctttt gactattcgt
    28561 ggtgattcat ctacccaata aatagtttcc ttcattctac aaacaattaa tttcttcgtt
    28621 attaaattaa aacacttcct ggaacaccga cataataaat aaaaatatac cctgataatc
    28681 atgagagaat aacaaatttg tatttatgaa aaactaccta tataaaaaaa acaagatata
    28741 acgatcttcg tcatatcttg cttcttcacc taactatttt tctccctata taacagcttt
    28801 acgcttctcc taatacacca ttcaaatata aatcgatcaa ttgcgccaca tttagcggtt
    28861 cttccacagg tcttttcgac cgagtgatgt aaggtgctaa cactaagaaa aaatgccttg
    28921 ctgcgatttc ttttgaaatc ccatttctta gcacagattg gtttgctgaa aagatttctt
    28981 caaaaggttc acgataattt ttcttccata gaaagaaaag atgatctctt gtttcctttg
    29041 aaagattgct ttcaagatcg tgaagcatca gaaacaaatc gaccagatgt gtctcaatca
    29101 aaaactgcgc catttttgtc aaggttactt gaaatcccgt ttcgcttgtt tgaacgattt
    29161 ctctcaaagc ttttccaaca tcttttaagt ggttttctaa aactgccgta tatagttttt
    29221 ctttatcacc gaaataatga tagagattgg gttgggtgat cccaactttt tgtgcgatct
    29281 ctcttgttga agtgccgaga tatcccttgg acataaacaa gtcagtcgct tctttcaaaa
    29341 ttttttgctg tgtatctttg ttgtatcgat ggaccatttt tccacctgct ttttggttat
    29401 tctttttctt tcaatacacc atcttccatc ataaatattc tatcgcattt gtcaatcagc
    29461 cgtgtatcgt gagtaaccat gatcgttgct ttatttttgt cttttgattc ttttgccaag
    29521 atatcgacta cttctcgtgc cttttctgaa tccaaactag cagttggttc atctgctaga
    29581 ataatggacg gatcattgta caatgcacga gcgattgcga ccctttgccg ttctccacca
    29641 gatagttctt ctggatactt gttgatcaac ttatccacgc caagctgctc aaataattct
    29701 tttgcttctt tttgacgatg ttcatgtttg atcttatcta ctaataataa ctgctttttt
    29761 actgtcagaa aaggaaccag attggaagct tgcaaaataa agccaatttc tttaaaacgt
    29821 agtttaacgc gttcactttc tttttgatta ctgaattctt gatcattgat aaatacagag
    29881 ccactgctag gcgtctgcaa tccgcctgcc aaagtcaaaa aagtactttt acctgaacca
    29941 gaaggaccga tgattgcaac aaattcccct ttatcgatac tcatggttgt ttctttcaat
    30001 gctttaacta cttggtcgcc atcttggaaa cttttttcta cttctttaaa ttctattgct
    30061 ttcatgctct tcctccttct tatccaattg ccttcaacgg atcgattttt acgattgtcc
    30121 gaacagaaaa tagtgcgcct aacacagcta ctaccagcat caacaagcta atgacgccaa
    30181 aaaacagcca attgctttgg aatggaaccg catcaggtag aacaagggat gttcccactg
    30241 ttcctaatag accaagaaga ataccaacaa atgataaaat gaatgtttgt gccactacag
    30301 aacgcgcaat aaatcctcct gtgatccctt gtgctttcat tacaccaaag atattaatct
    30361 tttgcatcgt cagtacataa atgaaaatcc cgatcacaat agccgcaata acgatcaaaa
    30421 atccgatcat aaatccaaaa gtcaatactt gcgcattata tccaggtaat tcattgatga
    30481 atttagaaat tttgatctgt tctaaatctt caggtaaatt actgatttta cctctgacaa
    30541 ctattgcgtt aatccgtgca ttttcacttg tatcttcttt ttcgaagcgg atctcttggt
    30601 aagcattgat ggttgtatag agaactggtg agacattgaa tttggcatga tccgtaaagc
    30661 cagttagttt aaacgtcttg tcactaccag ataacttaac tgtatctcct acggctagac
    30721 catattcttc tttcaaacta atatcgccta ctgcctcatc atcgttatca aaagcttttc
    30781 cttcaacaag attgggcatg atgaattgat ttttatcgat accgaagaag ctaacattga
    30841 tttttccttc ttctgtactg tctttactag tcacgactcc aggtgtctgg gcaagatacg
    30901 ccactttccc accttcgact tcttctgcta tcttcttagt aatcatggac atccctagat
    30961 ttgaatttgc atctttcgaa agcacaatcg aatctgcctc ccacttgtcg actgctgttc
    31021 gattatcttg agctaagcca taagctaacc cagtcaaaaa aaataccagg taggcgatca
    31081 aaaacatcac ccctgcaact aacgcataac gtaactttga atgcataatt tcatttaatg
    31141 ctaaaaacat ttaactcctc ctcctgaaat aatcatttta tacaacaatt ttatcacttg
    31201 ataaattttt ggcgaatatt atgctcaaaa aattcttccg ctatttcatt atatgaattt
    31261 tttattctcg attgattaat atttattata tccccacaca ataaaagacc gtgatatttc
    31321 tcacagtctt ttatctactc tattgctatt tgctctctta gctagtactt taaaagctaa
    31381 ctcattgagg tagtgaaaac gagaggtgtg ttcagacaga aatccaaaat cctcaactgc
    31441 tagctgtgga atcgtagttt tgtgtttcac gatatccgca agcaggttca aatctgataa
    31501 taagaattct tggcttatca gctgtccgcc aattatttct aatgtatccg gatggtagat
    31561 cagctttgca tttactgttt tgttttcttc atcgtattgt ggaaatagat caaattctcc
    31621 tgaacacgaa ccggtatttt gttccaagaa tggcgcttca tcttctgtca gccctacagt
    31681 tcctaaaaag tagtctataa tgactgctgc tgttatcttt tgtctgaggt cgtaagcggt
    31741 tggctgacca ctcaaagtta gcgctactgc tcttgccata tgtacagctc gagtaactaa
    31801 aggtaaaaag agatgccgtt tatttttgtc aaaactgact gggacgatat ctccaatcgc
    31861 atagatgtcc ggatcacttg tctgcagata ttcattgact atcacagtct catcctcata
    31921 caaagccact ttttctttta acaatgtcgt attaggacga gtttgggcag aaaaaatgac
    31981 catatctgcc tttattgctt gagatagaag ttgtacggat actactttct cttctgattt
    32041 tttccaatcg atcaagaatt catttggata aaaattgatt tgcttagttt gcagctgatt
    32101 ttgaaaactg ttgatcatat ccttatcgaa atatcttgat aaaacattcg gcatataatc
    32161 aactagatga ataatgtatc ctttatttct caaagcatct gctaattcca atccaatata
    32221 accagcaccg aagatcacta cttcttttgc ttcttttaaa gcggcaagta cttgcttaga
    32281 ttgcactaga tttttatagg tgtagacatt ttcagcttca atttgtttat acaaggtgac
    32341 aggagaggaa cctgttgcta cgattaattt atcataggtt atggtctcgt tttttccttc
    32401 tttggatgcg aggatgagct gatgtttgtc tggatcaagt tcagttactc tccattcggt
    32461 gatcagtttt gccccacttt cttgaagagt ccgcatgtta tgggcaactt cagctaaact
    32521 acgtatcttt ccttttaaat aaaggttgat tccatttgaa acatagccta gtccatcttt
    32581 atgatcttca tcgatcagta aaacttctgc tttcggttga agttttctta gatttagggc
    32641 agctgtgatg cctgcatggg aagcaccaat gatcacgatt ttcatgtttt attcatcctt
    32701 aaattcaatt tttctaaata acattctttt aaaaattggt tcaaataggg gaaatcataa
    32761 ttatattcgt tgctagtgaa tacaaaaact ttagcggact tctgtgacgg gtactctttc
    32821 aattggctag tcaaaatgat atcatactgc ttttctttct cgtattcgac taaatcatat
    32881 ttgatcgtat tcagcaattg ttcctggatg atttctttca cgatatccgc cattagcctt
    32941 tcataaggaa gatcataagc aatttgcaga cgtaattcta actgcttgat cagcgtccgc
    33001 aaaatagatg catagcgagc aaataattca aagcgggcat tttcctcgtc tacttcagta
    33061 aaatcaacaa tagcaaatgt ttgtctgacc aatctttcac ataaaaccag gaattgttta
    33121 tcttctactt cagttaaccg ctgttttaat ccttcttggc caaaaatatt catccatcct
    33181 ctaaaatagg caaaatgagc gtgattttgc atcactgtca acgcaatctt ttgcgaaatc
    33241 tcttctggaa aaacataaaa agagaaatat tcttctatcg attgtttaaa cgttcgattc
    33301 aattgagcta ctacgtcgtc tttgtatttt tcgcttatca aaaactcctg gacatgcgag
    33361 ttatgtgtat ctaatgtaaa gttactgatc atgaaaagat aaaaaaccaa cgtttcattt
    33421 tgattccaag gaaaggcata ttgaaagaag taacgggtca aaaatgtatg gaggtccaat
    33481 aaaaattgtg tcgtttgaaa caatccaaca ggaagattgc tataatctgg tttggatttt
    33541 ttcgtacagc gcattcgctt cttcataatg gaaagccata tttcaaattt caatcgtcct
    33601 tcttctgtaa tagtaaaact aaactctttt tgcagcagat ctaaaaaagt gtttcctgat
    33661 aaatcactgt attctttttt gttttcaaga taaggacgtc caaaccaaaa taattggaaa
    33721 taaaaataac aaatttgact ttctggaccg gtcagccgcc cgctttttat tttcagatga
    33781 aactcattta aagaatcatt taattccttc actctgcgag acaaagtagc cgaactgata
    33841 ttaagttcca tgacaagttt ctgccgatca aatgagccat tttccaacag ataaagaagt
    33901 atctgatatt ttgtagaata gcgcaagtag caatgataat aaacatcgac tgctgctata
    33961 tttccatgtc tgattaataa aatattccct tcttggataa caatttccaa ttgtttgata
    34021 ttttccaagt tagcttgtaa ataaaacaga tcttctttca aggtagcccg atcaactgat
    34081 aagcgggtaa gtacgttatt aacggaaatc tctccttttt cctctaataa aagttggaaa
    34141 agtttgattt gccgttgttc atttttttca agcaaactga aaagttccaa gtccactcct
    34201 ccaagcattt ctgattttaa cagcagaagt ttccttctca tccttgaaaa agaggatctg
    34261 tgacagttat cacagatcca tacaaattct aatcacttag ctggttggcc cagctgtcag
    34321 ttcccatgtg accatgcctt gatacgttgc gccaggttga gcattcgtac tgctatttac
    34381 ctctaagagt aaaccatcgg tatcgggaaa gtcaacgaga acctctcctt tagctgttcc
    34441 atcactctca taaatcaacg tgttttgacc ttgctgaatc aattgttttg atccagaatg
    34501 attgaagtat aattttgttc cgcttaattc ttgaccttga tcattcttaa ataaagaagt
    34561 caatgtccct gtcagttgcc aaggagacgt gcttggaagc cgagtatccc gcacatcaaa
    34621 agaaaaatcg ccttttgctt cataataact tgtcttcaac ggtttgggga gatcgttacc
    34681 aaatgtaaaa tcatctggta cattaactaa tgttaattct tgtttgggga caagaattgt
    34741 tttagcagat gtttggacaa ctgggttagg aagcttagca ccatctgcta atgtaatatt
    34801 gctggaaagt acagctgcct ttgtcagtat gctgacggat tgtacagaag tatctacagt
    34861 atttaattga tagttgtaag taagatgaat ggtatctcct attttgctca atagatctgc
    34921 tgggacagaa actccttgtt gaatgccggt atctgcgata gttgtaccat ttaattttaa
    34981 ggaaccattg atataagtag tattttcagg aagtgagatc gtgatttttt tgtcggttat
    35041 cgcatcccca gattttgtta acgtgaatgt cacattttct tgaatcgtat ctcctgatag
    35101 agcagacagg ggatctcctg tctcaggttg tctgctaagg ttttttagct gactatctgc
    35161 actcacagat ggaaaattag ccactgttat ttgaggaaga gtggtgattc cagcttgttc
    35221 agactgccat ttgtacgcaa aagacggata catgttagta agtacatcgt ccaccaagcg
    35281 ataatcaaaa taatattgcc ctgaagccaa tgcaggtagt gtagaagtag ctgtatttac
    35341 acccccgtta tttgattgga tcgtttggaa aagttgatct gtccatcctg tcgctaattt
    35401 ttgggttcct ttcgaataaa gcctgtaatg gatcgaaccc ttttttagtt cagttgctga
    35461 ccaattggcc gtgacttggt acgatgcaga agaaaggagc tttccccact gactatcggc
    35521 tgttaagttt gttggtaaaa ccgttccgtc tagcttgtac tgctccaata cctttaaagc
    35581 aacaggagaa agcaatccgc cttgcgcatc tttcccactg tacacgatat tggctgtctg
    35641 attcaatcca cttgtgcttg ctggagtatg agtcagtatt gctccttgtg taacgttttt
    35701 tcctgtaaaa tagttgacta cgcgatcaaa gcgactaaat ggcaactgat caaaacgtcc
    35761 ttcagtcagc cccatcgtca atgtccgact attggtagag cctggaaggt tctggttatt
    35821 tccatccacg tattctgtgg tatcagcaaa tgtattatat tttcctgttg aatcacttgt
    35881 tgagatgtaa ctattccacg tatttatctc tgcacttgca gaaaatgcat atgctcctgg
    35941 attcaagttt ccgccagtta atttaggata aaatctacct gcagctggag ctaaaatcga
    36001 attgaacata cgatcttgga taatattatt cgaatcataa aaatcaacag aaaaaacggc
    36061 ctttttagac atagaaggtt gtaatttacc cttggtcaaa attttgtttg gactcgtatc
    36121 atatttatct ccgttttctg tcatcttcgc gacattagct gtaacatcaa attgaagcgg
    36181 gatatcgtcc acgttgaatt cgcctcgcgt aataatagca ataatatctg ccaatagagt
    36241 atttgtatcc cacgacccca tcccaaaacg caaggtatgg cttcctgctg ataataaact
    36301 accgctagta accttgatgg gggcaccggc aatatacaag taggcgttgt tccagtcaat
    36361 tgcttttaat acatcactgc ttgatacatg ttccggcaaa ttgatgatcg ccacaaatgc
    36421 aaatccttgt gcagtgatat tcaccaaatc aagtacattt gttagtttca catggctttc
    36481 ccaatgaaaa cggaccgtgc cacctttcca aggatcattt gaaccatcgt tcaaataagt
    36541 agcagtcaat ttaggcaatt ggttagcccc agttagttgg acaagcttcc cgccattgac
    36601 tgcactacgt ccaccatttg gaggtacgat attttcatac gtgtatggat cataagaaat
    36661 ggcaggaggc gttgctccaa cagcttctgc cgaaggtgaa aagataaata tacttaaaac
    36721 aaaaagcgaa gtaaaaaaga aggagcgaaa aaatgaaaat ttattttgta gcaacatgtt
    36781 catcctcctt ttcctttctt ctccataaca acaaccacgc aagaagccat aattctatac
    36841 caatgatcaa ataaagaaaa cggatctttt cttcattcgt ctccgggaat acgccatgtt
    36901 gattagattc tttataaatc ggtagctgtg tctgctgttt tgatgcgcca ttgactgaac
    36961 cacttggatg cggaactgga cttagatcat aaaatgtaat cgtacctgaa cttttggtat
    37021 ctgcttggac tggctcttgc cagctgaaga acagcaaact tgcaataatc cctcccaata
    37081 agatcgatgt tttcaacgaa atcacctcat catgttgttt ctcctttttt ctttttcttg
    37141 tggacccaca aaaacagcaa aatcaaccat aacactagaa ctgcagcaac caatcctgca
    37201 taaagccatg ggaaagattg ttcttgtact gcaaaaacgg aattttcatt tactttttgt
    37261 gctttttctt ttttgatcgt aaagttttgt gtaaatcgcc attcattact gtcgcctgat
    37321 tttgctatga tttctgcgcg gtaatcgccg gcttttgctt tatcaccagc taaatcaaga
    37381 ttcaagtgaa aattcgaatt tggtgcaact aaaaattcat tagacgtatt ttcaatataa
    37441 agctgatttt cataaaagat cttgctcgta aatatcaaat tgctgataat cctaggctga
    37501 ggattttgga aacgaagctg aacagttggc tttgcgttga cctcgtccaa atctcctgat
    37561 aaaagtttca gatccggttc tatcgtctct tgactttctt gtaaaacgac tgcgagatta
    37621 taagcaatac gatttttgat ttgtgctcct tctgtagcat tttctgtttc agcttcttct
    37681 ttaggtgaaa catgaatacc cgctaataca cgtcccttaa agctttttgc tgggattttc
    37741 acttctatag gtatagttgt ttctgcatgt gctgatacat tgatttcctt tttcgcaatg
    37801 agcaaaacgt cacggacatc aaacggcaat gtctcatcta acgttgggtt tttttgacta
    37861 taatcgatcg ttccaccatc actcgttacc gccggattga cagagacctc aaatgttcgt
    37921 tcttcatctg aagaattgct gataattact tctagtgtct gattttgttc cggtgccact
    37981 aataaatcaa aataaccgat tgctttatct atttggttct ctggaaatac cggtttgatt
    38041 ccaaagtctc ctgctgaatc ctttgcttgg ctgattgacg gaaaaatgaa aaatagactt
    38101 aaaatagaaa aaaacgaaac aaacagtcgt ttactttgat tcattgtcca ccacatcctt
    38161 tcaacaacaa ctgcggtaaa aaatccttca cctatagtat tttcaccgca gtttgttgaa
    38221 tttatttcat actctatgct actgggccag ccacgaggtt ccaagtcaaa tcagccgtga
    38281 atgttccagc tgctgctttc ggcactttca gtgtgatatt tgctggatca taaacagaaa
    38341 gccatgttcc cagaccttga tcttttgctg ctgtaaatat tggtgtcgct gcacttgctt
    38401 tactcaaccc agttacagtt tgactagttg gtccagtgga ttcgttatct gacgtacttt
    38461 taacagtgga tgttccaaaa tctaattcag cgccttgcaa aacttgtgaa ccgtttacaa
    38521 aatctgaaac cgaaactgtc acgttccacc cttgtgcttg gccatctgca cctacacctc
    38581 gacgatctga tacttggaga taaggtgtat cgttttttga agcgtctact tgatacgttt
    38641 ttgttccgct ttcaatctcg tgtgtaccaa atggcaattc aggaactaca tccaatgtta
    38701 aagaacctgt ctggccagtt gctggatttt ccggatctgc tggctctact ggttttgaag
    38761 gatcagtagg gttaagaggc tcaacggtat ttgttggttc agtaaaggta atatctcctt
    38821 ttgaagtcgc ttgaccctca gcaaaagctg gtgttgccaa gccgcctaga agaagtgttg
    38881 aaagtaataa tgtcccaatc atgttctttt tcataataaa tcactccttt ttcttagtga
    38941 cctaagtata gtagagacag tatgcaaaaa atcctcatga attcatccgc ttacttgaaa
    39001 ttatgaccct ttttgtataa aagagaaaaa aacaataaaa tcgtttacat gaatattctt
    39061 ccttttttat taagtatgaa aaaatatttc aattttattt tttcgtttca aattattcta
    39121 ttagtgacat ccttgaacta taaaaatagg agtacggcat catctgccgc actcctattt
    39181 ttttcgtcat ttatttctta aataaaacga ttctcacgca attttatttt tttgatatct
    39241 tctactgttt gtgttccagc taattgcatg accatttcca attctgtttt aaagaaatca
    39301 aacacttgat ggactcctgt acttcctcct aaagacaatc cataaataac gggtctgcca
    39361 atagcaacaa gatcggcacc tgatgcgatt gctttgaaaa catgttgtcc acgtcgtaca
    39421 ccgctgtcaa acacgattgg tacacgttta tctactgctt ctgccacata ttgcaacgaa
    39481 tcaaacgcag caggcccccc atctaattga cggccaccat ggttagatac ccaaattcca
    39541 ccagcaccag attctaatga tcgatagaca tcttcttctg attgaacacc tttcacgtag
    39601 actggcaaat cagaatgagc agcgataaat tctacatctt ttggactaag tttttgtttg
    39661 gaagatttat atacagcatc catcgtttgg cctactcctg attgataagc ctgaacgatc
    39721 ggcattggca gcgggaaggt aaacccgttt ctgcggtctg tttcacggtt tccgccaact
    39781 gttgcatccg ctgtcaatac gattgctttt gctccattac gcttggccac ttcaagaata
    39841 tccaaattga ttccattatc tttgctcata taaaattgaa accattgcgg tgctttctcc
    39901 ccgcctgctt ctcttatctc ttctaaagta caagaagcgt aagaacttgc tgtatagatc
    39961 gtcccaaaat ctgctacacc ttttgcagaa gctttctccg ccttcacatg agctaatcca
    40021 tgagcagcaa ctggtgccat gatgattggt gcagtcagca tttcttcgtc aaaatgtgta
    40081 gttgtatcgg gtaattctac atctcttaat acatgaggaa taatcaaacg atgattgaat
    40141 gcccgttcat tttcttgata agtaaataag tcgccagcac cactgctgat gtaaccataa
    40201 ccaccagctg gaatcacttg tgaagccgcg atttctaaat cttccatatt gataaaatct
    40261 actattcctt catttgtacc tgcttgatat gttttttcca tctcaatgcc ttctctctat
    40321 aaaatttgaa tgatgaacaa tatgatagcg tttacacttt ttatattact cttcgacatt
    40381 gatacaggca aataatctgt ttacatttca gccattccag atgtcactac tctggaagaa
    40441 atgattgtaa taaaaataga taaaaaaccg atgggacttt gacaaaagta gtgtcaaagt
    40501 cccttttttt gaataaacgg tgttcaaaag ccattttctc ggtattaagt caaatttatg
    40561 caaaaatgtg gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
    40621 ttcacaacct ctcgataaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    40681 tggcaaaata tgaggaacta ttttgccatt tgtctctcta atactcaggt cagaacgttg
    40741 acttcacagt ctcttcctta aacggtgttc aaaaacttgc atctgcggta ataagcccaa
    40801 ccaaacgaaa aataggagga gctatttttg tttggctgtt cttattactt gatgcagggc
    40861 aggtttttca cgacctctga ttcacggtgt ttctttttca tcaacatttt tacgaaatac
    40921 tgtggaagac tattgaaata agtgtggatt gtttctttgt gtttcaatcg tttcttgcca
    40981 ttcttcattt aattttggat taccgttttc ttccttgaac tcataaggtc ctaattcaaa
    41041 aatcccagac agtatttgat tcacgatttc atatagttca gtgtgatcag acacatgatt
    41101 cttgaagatc atctcattat caccacgttt gaacctcaca ttcgggctct catgatgaac
    41161 ctctatgcat ttttctcctt tttttaactg acggacttct gaaaacatct cataaatatc
    41221 ttctccagta aaaccttcag caagcgccag actgtccaca agaccgccta ctgtcagttc
    41281 agagattatt gcttgattgg aatctaattt taatgtatag cttgtcgctt ctactacgcc
    41341 acatgcattg caaccgcctt ctgatagttc gctttttcta atcaattgaa cttctttcat
    41401 tttctcttcc tcctcttaat tttctactta tgtattttta tcacctgaca taggtgactc
    41461 ctatcaacgc tatcatgtgc ttttttcctc agaaatccta tttatccatt ccatctatcc
    41521 cttattcttc tacttttctt tctctagcat ataagagata cgcatagtat tcaagcaagg
    41581 attgtcgata taaaaagaac aaatactgct attttgattt cttttggcta tattacatat
    41641 aaataaaatt tatcatacct gctctttttg actatatcat catctgactt ctatacttcg
    41701 tttgtattaa aacttaggag gaatcattca tgcttacagg gttaaactta ttaaataatc
    41761 cttttctaaa taaaggaaca gcttttacaa aagaagaacg ggcaaaatat ggaatcactg
    41821 gtatgcttcc aagtactgtc caaacactgg aacaacaatc tgttcaagct tatggtcaat
    41881 acttaagcaa acaaacagat ttggaaaaac gtatcttctt aatgaatcta ttcaatacga
    41941 atcggacatt gttttataaa ttgatgggcg aacatttagt tgaattcatg cctgtcgttt
    42001 atgatcctgt cgttgcggat tcaatcgaac aatacaacga aattttcttg aaaccgcaag
    42061 atgctgcatt tctttcaatc gacgaaccag aaaacatcaa agcaagtttg aagaacgcgg
    42121 cagatggacg agacattcgt ttgattgttg tgactgatgc agaaggaatc ttaggtatgg
    42181 gcgactgggg tgtgaacggt gttgacatcg ctatcggcaa attgatggtc tatactgctg
    42241 ctgctggtat taatccagca caagttttgc ctgtatcgat cgacgctggt actaataatg
    42301 aagaattatt gaataatccc ttatacttag gcaatcgtca tgctcgtgta gaaggcgaaa
    42361 cttactatga attcatcgat caattcgttc aatcagctac cgaattattc ccagaattgt
    42421 tattgcattg ggaagatttt ggacgaggaa atgctgcagc gatcttagaa aaatacgaag
    42481 atcaaatcac tactttcaac gacgatatcc aaggtacagg aattgttgtc ctagcaggtg
    42541 tattaggcgg attgaatata tccggtgaac aattgaaaga tcaaataatt ttgacatttg
    42601 gtgctggtac tgctggtgtg ggtatcgcaa acatcctttt agatgagatg atccgtcaag
    42661 gtgtacctga agaagaagca cgccaacatt tctatcaagt agataaacaa ggtttattgt
    42721 ttgaagatac agaaggattg actcctggtc aaatcccatt cgctcgcaaa cgctcagatt
    42781 tcactaatgc tgatgaattg acaaacttag aagcagttgt aaaagcgatc catccgacaa
    42841 tcatgattgg tacttctacc caacctggag cattcactga agaaatcgtg aaagaaatgg
    42901 ctgctcatac accacgtccg atcatcatgc ctttgtctaa tccaacaaaa ttggcagaag
    42961 caaaagcaaa agatttaata gaatggacgg acggaaaagc attagttgga acaggtatcc
    43021 ctgctgctga tgttgaatat aatggtgtaa cttaccaaat cggtcaagca aataatgctt
    43081 tgatgtaccc aggtttagga cttggtttga ttgcttcaac agctacacga gtaaatgctg
    43141 aaatcatttc tcaagctagt cgagcattag gcggaatcgt tgatgtaaca aaaccaggag
    43201 cagctatctt gcctccagta gcaaaaatca ctgaattttc tcaaacgatt gctgaaactg
    43261 tcgcaaaatc agttgtcgca caaaacttga atcgtgaaga aatcacggat atcaaagaag
    43321 cagttgaatc tgctaaatgg gttcctgaat ataaatcttt agaagattaa gatcgttata
    43381 gtaaaagaac atacagaaac tttgtatgac gtctaaaaag aaaaaagcga ctgcggcaaa
    43441 acagcggcca gccaactcct gctcttttgt cgcttttctt tggctttttt catatgatac
    43501 agaataagaa aggatctgta aaaatggcac aaaaaacaac aactgcaact gcagttgagg
    43561 aaaagaaaaa agaaggtttt tggtccacaa aaatcaccgg cgtaagtttg ccactttatt
    43621 tgatcatggt ccttgtcctg atcattgcaa tggctttagg gaaattaccg acgaatatga
    43681 ttggaccaat cgcagttttg gttattttcg gtaatttatt tcattttatc gggacaaaaa
    43741 tccctatcgt caaaagttat ttgggtggtg gttccgtttt tgcgatcttc gcttctgctg
    43801 cgattgctac atttgggatc ttacctgaat atgtagtgaa atccacagaa aactttgtca
    43861 gcaatatggg cttttgggac ttttatattg ctgccttgat tactggtagc atccttggta
    43921 tgaaccgtaa tctattgatg aaagcttctg ttcgttttat tcccgttgca cttatttcaa
    43981 tggtcgtctc cttttttgcc gtaggactcg tgggcatgct tatcggaaat ggttttgcaa
    44041 attccgtttt atacatctca ttacctgcga tggctggtgg tgttggagcc ggagccgttc
    44101 ctttatcaac gatttatgca aatgttttag gtacggatgc ttcttctatc atttcccgcc
    44161 tgattcctgc gacagcaatg acaaatgtct tagctatcat cggtgcagca ttagttgctc
    44221 gtgctggaca atcaatgcca aaattcaacg gcaatggtaa attaatggaa aaaggcgatc
    44281 ttggcgatgg aaaacctcga actgtgaaac cttccattcc acagttaggt gttggtttga
    44341 tggtatcatt gactttcttc atcttaggac aaattggtaa ctattttgtt cctaaagtcc
    44401 atgcttacgc atttatgatt attatcgttg tcatctgtaa aatcgccaac ctattaccag
    44461 agtattatga agatgcagct atcatgttca ataacttgat tgtcaaaaac ttgacagcag
    44521 ctgttcttgc cggtatcggt attgcgttgc tgaatctgaa tgtattagct tctgctttga
    44581 catggcaatt tgttgtctta tgtctgacaa gtgttatcgc gatttcagtt gtttcaggat
    44641 ttgtcggtcg tttgtttggt ttatatccaa ttgaatcaac tattactgct ggcttatgca
    44701 acaactcgat gggcggaaca ggaaatgtag ccgtcttatc tgcagctaat cggatggaac
    44761 ttattgcttt tgctcaaatg ggcaatcgac ttggtggtgc catcgttttg atcatctctg
    44821 gtttcttgat gcaattactt tcttagtcaa aaaaacaaaa atacctgatc atcaagtatc
    44881 ggcttggtga tcgggtattt ttttgataaa gatttacagg attttcaaaa atcttttttg
    44941 cattttatcg gaatgataac cgatgtgttc ataaaattgg tgtgcttcta ctctgtattc
    45001 tgcagaatta agccttattg tcgttattcc tcgttttttc gcttcttctt ctacgccagt
    45061 catcaaagcc tttccgatgc ctcttttttc agcatattta gacacagcaa gtgccataat
    45121 attgaacatt ggatcgctaa aaatcgattc ataggactcc gcttgtacat agccagctgc
    45181 ttttttagaa gtaggatctt cataaaccaa gaaaaaattt ttctctggct ccttcagcag
    45241 tttcttcatt tgtctcaccg taacggttaa tggtatatca taccctaatt gatccgcatt
    45301 gatttcacgt agtgcttctg catcttctaa acgcatttcc cgtaacatcc aaatcaccag
    45361 ccaatttctg attttttaaa atgaattttt ttatcatagt atactctttt tttcatttag
    45421 aacaatatgt ttcagtgatt ttgacttgcg ttagtctaga ttctcctaat gtttgtaaga
    45481 tgacaaaggc attgtaacaa ttgaccaaaa gaaccacgag gacaattact tgaaaaataa
    45541 aggtcacttt ttttggagac aaaaccctgc taaatcttgc tccccacaaa cctccaataa
    45601 cagcagcggg gataacataa aacaacatag ataaatcaaa gcgcataaag ctacttgtta
    45661 aagcaatcgt taccaatttt gccagttgag aaaagaaaat cgtagataga gaatatagtg
    45721 ttgcttcttt tattggcaat gcaaacatca gcatcaacaa agaaacattg actggtccac
    45781 caccaatacc taaaaaacta gctaaaaagc ccagaacccc cccacatata aggtaccaag
    45841 tccatgacgt caaatgaaat tttggctgat gatgcttggt gtagaagaac gcaaaaacca
    45901 aggtcaatac cgtcaaaaaa atttggatca gctgcacatg cttctcgttt tcaaataact
    45961 gtaaaaaaac ttcgaataca atgttgccga ctactcctcc aacaacagct ccactagata
    46021 cccaaccgac aatctgccaa ttcagtgatt tcccgcttga tacttgtcgc acagtcgaaa
    46081 caatggacat cgtgaatacc gcaactgttg agtaaaaaga aatccctgca acagaatggg
    46141 cgccgataag atcaagaatc ggtttgatca acacgcctcc acccatcccg gaaatagcgc
    46201 caactgtgtt cgccagaacg atcaccacaa aataaatcag accaatcatc ttgtatttcc
    46261 tttctcttgt ttatatcttt tagcgattgt tactcttttc ttttattata cagtaaaaga
    46321 aacaaacgag gcttcgaaag cttttctact ttttaaggaa atttagtgct aaacgaaaag
    46381 aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagctcct
    46441 cataattttc gtttggttga acttattacc gcagatgcaa gcttttgaac accgtttaag
    46501 aaagaggtcg tgagcctgcc gttcagctcc gagtcacaag gaacaatttc aaaaaacagc
    46561 ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    46621 aaggaagaga ctgtgaagtc aacgttttga cctgattatt agagaaacaa atggcaaaat
    46681 agttcttcat atttcgccgt ttttcgagcg aatgccgagg ggcagctgat tgaacatcgt
    46741 ttatttggaa aaagggattg tgacaagacg tttgtcacaa tccctttttt tatatcttat
    46801 taattcattc ttttgttcta ttcttcttct tccgccatga atgagaaacc catatcaaag
    46861 aactataaag gttatcaaaa cagcgtttat gactaaatga attagagtta tctatcacta
    46921 tttttcaagc gatttaaacc aaatggcata caatatggca tacaaaaact tcaataagcc
    46981 gtgggggttt ttaaatttct tcttgttctt taaaatcaat gagtggagct acataatttg
    47041 aatccacttt ttcaaacgtc actttttgca tcgacgaatg ttttctttcc caagataacg
    47101 taaatagatt tgactctgga cttataaagt ctacttcacc ttctagtatt actttcttat
    47161 gcaaatattc ctctaataaa tatagacttg ctaaagcata caacacattt tccaaatttg
    47221 cttccttata atttgaagaa cgaccatgtt ttactctgtt atatattttc caccaatctg
    47281 gtgtatcttc aggccaccca tctccaaaag gttttagtga agtcaatgtt aaagaattca
    47341 taaccctaac accattatcc aaacacatta attcgctaga tttaataaca gctttaaaat
    47401 cagttatgct tttattgcta ctgctatcaa atccgcaaag ttctttgaaa ataatctcac
    47461 actccgaacc aatcgctagt agtagttttt gatattcaat agagaatgtt ttgaaattat
    47521 tctcatcgag catcacaaaa tctgtggtat taataaactc attttctaaa tgtagataat
    47581 acttccaata aaaatttgta aactctgctt tgtccattaa atcacctctt ttgaattata
    47641 acaaaaaaaa cacgtccaca acagacgtgt tctttgattt ctacacaatc agcttgccaa
    47701 aaccatccaa tcagtcctag catgcctgta ttaaaattga acgttcatca tctatcatct
    47761 ttcttagtcc agcatgtttg caaccacacc tacaattttc agtccaacaa tagatttcga
    47821 ctagctaagt cttgcaaaat tcaggcagct aacccttcat ttactacctt aattatacta
    47881 aatacccaca acgtttacaa gtgtaatcat tctaagaaat ctgtttagct atgatcgttg
    47941 tataatcttt aaacgccgtt ccttcattga ttgaaataat tttataactc ttgtttttcc
    48001 aagctacttt catatcgttt gttagttcat aatcttgatc gtatcgaata ataaacgtca
    48061 acgttccttc aagaatggta cctacactag caagcacgtc ttttagcatt tgtgaacgca
    48121 cattagccca acattcaaat acagtttctt cactatccac catctgccca tcttcatctt
    48181 ttacccgttt tgtcttttta aacttgactc tctcagtcaa ttcatttaca ttcttaatga
    48241 ttggcataat aagcacctct catttgttgt actaatgctt gtaccgttac tggtatccgt
    48301 tcgctgctgg cttcttgacg gttcaaatac caatgttgag taagtaatga caccgccaaa
    48361 tcaaattgct gataattatc catatcttta tccgttgcgc tactatcaat agcacttttt
    48421 atatattcgc ctgctgtatc aatcaattgt tgaataaagt catcatcaat agtgtgatcg
    48481 atccgcatac ttttttttat gctatccaaa attaccattt tatctctcct aatgaaaaaa
    48541 aggcagtggg ataaaccgcc gcccaatatt tttctatact tttggtgtta caactggcgt
    48601 aaactcaata tacactgaag cattttgatc aattttctta taatcgttgc gaacgatcac
    48661 tgcaagtcct tgtgagtagt aatcgaattt ttcccattga gtcgttactt gatttctacg
    48721 agcaacaaag atagattgtg caatatcccc cataatcata gggaatgttc cagctttagg
    48781 attggcaaac aatgtatctg caatcaatac tactggcata ccgaataatg atttaccact
    48841 aggtgctgtc acatcgggtt gtaaaatgta acgtccatct gaatctttca atgtatctag
    48901 gtggtaatat ccgctttggt ttagaattac cattttattt aatgctgggt ctaatgccac
    48961 attgtatagt tgtttcaaat cgtccaacgt agcagccgtt ttcttagtga atgtttttaa
    49021 taaatccatg atatgcttat tgtcagtgtt ttctaccaat ttcgctaact gatctttgac
    49081 ctcttgtaca atattcactg ctgaatcttc cacaacctca tttgataagg caatcttacc
    49141 agcacgagtt tctactttat aatcaactga agtaaacatt tcagcgtcaa tatcaccaat
    49201 ttcagcaagt tcagcttttg ttgctaacac tgcttgttgg ttagttgcta ctggatattt
    49261 accttgtcca tttgatactg tttttactgt tgcatattga gctaagttat aatttgaacg
    49321 tttcaaatca aagacttcac cgattacttc ttcgggaaca actgccgctg catttacagt
    49381 agtgactcca tcacgcactt cgccttgtga acggatatat tcttcatacc cacgcaattc
    49441 tgttttttcg ttatcgatta aagttttttc catgttatta ctctcctttt gtgctttaga
    49501 agtggattct aagctattgt ttgtttgttt tgcttggttg ttactcataa acgattcgta
    49561 agaacgctta tttacttgga catttgatga atcataagcg ggaacggtca cgacactaat
    49621 ttcattaagt gctttgattt tatctattga tcgttcaatt gtgccatctt cttttttgtc
    49681 gaatgaatct aatccaagca cgaacccaaa ggacatagaa tcaacaaccc cttttgagat
    49741 attctcatac acatcatttg aataggtcgt gtcggttagc tctgcttcaa aatgtaatcc
    49801 tgtatcatcg acattcaatt tcaacgtacc tgctttgaca ctagctagtg gctttgaata
    49861 atcgtgtcca tataataaga acacatttga taaatccacc tcttttaatg cttcgggtgt
    49921 aatcacttct acaaagccgc ctaaatcttt tgacggttgc ccgaatttca acgcatagcc
    49981 tgaaatagtt ttgccgtctg ttggttgttt ttcattttct ttctctactt tgggagaatc
    50041 ggctgttagc tcagcttctt ctgtcagccg tttttccttt tcttcttcat tattcaactg
    50101 gtgtaactcc ttcctgttct tttaattgat atttctccaa cgtatctaaa tacgtaaagt
    50161 tcaaacttgc tagtaaacga tcgccaccat ccatgggggg tagccccatt ttcgaccgcc
    50221 cctcattaat cgtgagaagt gaccctttga cctgatccaa tacatttttt acttttgttt
    50281 caggatcagt ctctagtaac tgatcggaat taaagcggat attggtttca agtttcgtat
    50341 ctaattcact cacaaacaca ttaaagtaat gaatcagcgt actttggata tactggagat
    50401 tactttgaac ggtgctagaa tgttcatttt caacgcctaa acgctcaatc ggtacaccaa
    50461 acgccttggc aatttgtttt gtgttccaat cattggaatt gataagcttc aaaacatcgg
    50521 tgtttatttc taatgttttg tagtccattg tttcatcaag aatgatcgtt ctaagagcat
    50581 tatctccact acttgaaccg ttggcttctt caaacttttc acgaattgcg ttctttgctg
    50641 atccgtctaa atctgactta tgaaccttca aaatacctga cccactgaca ccacgagtaa
    50701 agaaattgtg caatgtgcgg ttgccagctt gttgaatttt cagttcatca cgtaacgcat
    50761 aaagtggtgg taatcctgtt aaaccatctt gggtgaaata tttgaagtgc aaaatatcgc
    50821 tagacttcac acgtcttttt ttatcaccaa tctgataaga caacgtacca ttatctaatt
    50881 gagtaaccgt tacacttgag ttaggtaata agtgaatttc ttctaccttt tcaccgttgc
    50941 gtttaatctc tgcaaagcta ttaccgttta gcaacatatt gaccgccaaa gcaaacttta
    51001 aatgccaacc atccatttct gaattaggtt tctcgtttag taacttcacc agttcattat
    51061 cagcttgtgg catattgttt ttgaccaatt gaatcggact tgaagcaata tcactagcaa
    51121 taattcgaac ggctgcaaac acatcactat ttcttaatgc acgaacgctt gtaaagctgg
    51181 tggtgtaatc atccgattgg attgatacca catgttgtag gaaaggatct cccgttgtat
    51241 tttgtctcgc tttaaaaaat gccatctttt aacctccctt ctgttctgta tttagcatga
    51301 ccgctacccc aataagcact atgcccagcg ctaacaagcc atagtagaca tttgttagta
    51361 aggtgatagc aaccaacaca cacaccaatc caagaagtaa aagaacggta tgaatgtaat
    51421 tagaaagaaa attcttcact tgtataaaat tcattgtctg ctttcgctcc ttccgttgtg
    51481 gtgaagtaat ccattgcaaa cacataggca ttgattaatg cagctattgg atcaattttg
    51541 ttactgttct ttgatttatt aatctgaatg ccgttgttat ccgtttttaa aatagcgttg
    51601 ttcactgcat gggtgagaat cgtattttta ttgtgaataa cgttgccttc ataaacttgt
    51661 tctctaaaag ttcgggtcgg aacgtttaga gtaatcgttc cttgtcttac ttctaacatt
    51721 gggtagttgg ctttttcagc tttactgata agcgaattag cgttatacgg gtcataacaa
    51781 atccccatac attccaaatc attttcttga atcaaatctt gaataaactg aaatacttgg
    51841 tcatagtcca cgattccact ttctaattga gtaatggaac actcaccagc tttttctagt
    51901 tctcgataag gcaaaccgtc gcgcttttct ttgtcttgaa gcccatattt ggtggctaca
    51961 aagctatgtg aatcacaata aagttcgccg ttatctagcg gtacgatcca tgaaacgcta
    52021 gttaaatcat ctgttttaga taaatccact ccgatataaa cgggcttgcc tgtaatatct
    52081 tgtggttcta cttcggttgc ctgccagtct tcagcaatca tatagctgtc ctcactcgct
    52141 tgtctccata aattgaagtt tttcaccaat acagaattaa ggttattttg tttcaaagca
    52201 aggttcacat catcttgaat ggttggaatc attacttttt tgatttcttc actttcaaaa
    52261 atcggattgg ctttgatcca tttctcttga tcgtgaattt cttcgggatc gtccaattcc
    52321 catatcgcta taaagtacct gtcggcttgt tcttcgcctt ttagcacacg atctaacaat
    52381 aagtattcct cgtacatagg aacgtttaaa tcaagcccag cggtactaat aacacacaat
    52441 aagccgttct tttgttgggt cattcctgac ttaatgacgt tgtacgtttt acgagttttt
    52501 gcttcgtgcc actcgtcaag aattgcggtt gttggtgcaa acccatctag cgtgctggtt
    52561 tcactagcca gtgccatagc gaacgaatta gagggcaagt gctgaatctt agagttcata
    52621 attttcagtt gtggtcttaa aaacttacta gacttgacca cgtttctaag tgaattagac
    52681 agcatatcat agcccaattt agcttgtttc aaagcgttac ttacaaacaa tacttgcctt
    52741 gcttctgctg gttcttgttc cataatcaac gcattggcag ccatgccact tgcgatatac
    52801 gttttcccat tcttacggct catactggta aacgctcgat tgaatcgcct gtaattccct
    52861 tctttagtac gccagccata aagactgcct agaatgaatt tttgaaatcc taacatcttg
    52921 attgctttac cgtccgtaga tggtagcaac tcaatgaatt taatcgcttt ggtggcttgt
    52981 tcttcatcga acacataagg gaagctatct gacttagaac gctccaaatc gcttatatga
    53041 cgctcacacg cttggataat tttcttgggt gcaatcagct tacctgatag gactttctca
    53101 atgtaatcat tcatgaattc atcaactctt tgaacgggtc ttcgggttca tcatttttaa
    53161 ccttgttcaa agctagtttt gatcgtgatt ctaaggtcat acctagtgag gtggcttgtt
    53221 ttttcaaatc actcatagct tgtgattgaa cccgaaccgc tggattggct ttctttactc
    53281 cgttttcgca tgtgataaac gtacctgatt ttcttatctc ggcttcggct gttttcattc
    53341 gagcaaaggc taaacaatag gctaccaatg aaccataatc tgcttcactc aatggataat
    53401 ctttttttaa tgttggtacg atacgattcc attcacttct agcgctcgct gccatccagt
    53461 cagggggcgt gatatttatt aatggttgct gcttgaacaa ttcttctcta gcgtccacac
    53521 ggattgcttt ttcttcactt gatatattgc cttttgtatc gtgtaatatt ttagggtgtc
    53581 ccatgttgtc ctcctttcag acaaaagaaa aaggaacgta agcaattaag ctcacattcc
    53641 ttcgtttttc gatcagaatt tttctttatc taatttatct atataaatga tttttccgtt
    53701 ctgcatttga atggtcactt gaccaaaatg aggtaactca atggtgcgct gtttccctgt
    53761 tttatcagta ataacaattg tatttttcaa taaatctcct actttcatta tatacttttt
    53821 cacttgtgat tacaagtgta atcgttattt gtaattaccc ttatgccttt tcacaacggt
    53881 tatttgtacc aaaatgagta ggcatcgatt gtcagtaagt ttcacactat gcccccttaa
    53941 ctcaaagggt actcttttct cgtctttgtc tttcattggc tgtcttggaa ttatggtgtg
    54001 catggcataa agaacgaaaa ttagtttcat ctaaccgtct attccaatca tcttttagtt
    54061 caattttgtg atctacaaca tcagctttcc ttatcaagcc ttcttctagg caatcctcac
    54121 atactggatg atttaaccga tacaagtatg aagcctttcg ccatgttcta ctttgataga
    54181 attgaaaata tttaccttcg ctttgctttc tttcttcacg cttaattgcc gtttgctttg
    54241 ctttatgctt cccacaatac ttctgcctat aatcaatcaa tgttttacat ccagcatgat
    54301 tacattgttt tttaactgcc atagattacc aaccaaagcg atctcgttcg tcagaatcat
    54361 cttgttccca ttttgatttg tattcaatat aaggcgaaac tatcttcaag cacgttccgt
    54421 ctttaaaatt aactttagtg attttatctt cctcaaaact aataaagtct acatcttctt
    54481 gagtgtttga atactcaatg tgtttctcac catcgaagta tctaatactt tccatatgca
    54541 taattattct acctccaata aattagttat caattcatca tctgttagta gcaaagccaa
    54601 gaacttatca tggatgttta aaaacaaatt atcctcattc gatgtaagct ctcgttgtct
    54661 cgctcgatca tataggttaa gcagacaatt aaattcatcc attagctcac ctgtatcatc
    54721 atagttgcca ctatctacca atgatcccac tactagcgat tgacaccacg ctactgactc
    54781 ctgcagccaa tcaggttctt cttccattat ctttaatact tctggatcaa atgtggaata
    54841 atcttctgtt accaatccca cactaaatgc cagcgtgttc attagaaacc gatccatttc
    54901 atctgtatat tgaaagttcg gtgctttgtt tgttgaaata atattgagca tttactttcc
    54961 tccttgattc gtaacacgtg gtaacaccta cgtaacaggt acccgttacc actcaatccc
    55021 aacggggaca agggttctag cgttttggta acaggtaacg ggtaaaatcc agtaacatta
    55081 ttccccagca tattagctaa ctatttcaaa agttttaagg aaataaggtg ttaccccgtt
    55141 accaaattgc tgtatccctt ggggcagtaa gggtaaacta gtaacgggct aggtgttact
    55201 gacccgttac tacccgttac tttttgtact ttccagctcg atttttattt tcataggcat
    55261 ttgacttatt gtaaaatcct cgtcgtttat ttctttttgg cagttgcttc cgttcccaac
    55321 caggaatact gctaataatc aaggcaatct ttttagtttc tgcatctgat cgaccattca
    55381 ataaatcctt tgcttgttta tcaaaaacag cttccaaaat atcagcggtg agtacgctat
    55441 caactaaata aaactcttgt tgcatagtag ctccattata gaaatgctgt aaatttttct
    55501 cattacattt actgtcagga tagttctgat aataagaacg tctcgcccac gctggagctt
    55561 cttcccattt atacgggatt tccatttcca aatatttagt gattgcttct ttaattggat
    55621 tctccatctt ggcatcctct tgatagtctt ttgcaatttc taagacttct ttatcttcat
    55681 cgggactaaa atagattcgt tgtcctttat caaaaagcac ctttgcttca gctaacactt
    55741 gaagaaaata ttcatccttt gttttaaacg gattcatctt tggcttattc ttacaaggca
    55801 aaggataaaa acggcggttt cctgtatcat ctttcaaata ctgcccatca ttcgacgttc
    55861 caataaatac acattgcctt gcccaagcta tgacattccg accataagga ggtctaattt
    55921 tatcgatctt ggcactgatg aagtttttta tctgttcaat tttagttttg ttcattgaag
    55981 ataattctcc aagttcaata attacattgc cttgaagctg catataatca tctttctttt
    56041 cacctagcga atctaatgaa tcggtgaaaa aatcatccgt gtaaagtaat gctggaacgg
    56101 tactcttacc aatcccttgc ggtccatcca aaactggaac gatctcaaac tttattcctg
    56161 gtttatagat acgtgcgact gatccgacta tccaacgttt tgttacttct cgtgtgtaaa
    56221 ggttgtcgtc tacccctaaa aggtcaataa acagtgtttc cagtctaggc tgttgatccc
    56281 atttaacttt ttcgatacgt tcttttactg ggttgaaaac attctttcta gccacattta
    56341 ctactgcttt gtgtattttg ttgtcatcaa aaactacatt gtagttttct tcaatatatg
    56401 ccgttaaggc atcctcaaaa ctgtcttccc attctccagc ggtacgattg atccaaggag
    56461 aatcttttaa taggtgaatg tatccgctaa attcgttata agcaattgtt ccagctaaat
    56521 tgctatcatt ggttaaaatc aattctgcat tcacaacact gttactttta atctgatcgt
    56581 ctttattgca tcttaatcgt gacatccagg ttttcaattc aatgatattg tcttcacgct
    56641 cgctggcttc gggaaagtct aaaatactat ctaatttatg cttatgagcc atgagctttc
    56701 cccctcaaat cttttttaat catcgatcga ataatttgat tcacttcttt atcttttagc
    56761 ggtggttcat tgtacgcatt tgctgtataa atcaattctg ctaaagtttg attatctaca
    56821 ccgtgccata gtaaccaacc gcataactta gttaaatgat cgtttctact accttcgcca
    56881 gcgccttgaa caatcatatc taattttttt gctgtccctg tttttgctac gggtcggtat
    56941 tgagtcttta tttcttgcgg tttttttagc agtaggttca ctagccaatc gggagctggt
    57001 gcaatattat ttagagatac gttttcaata ggttcatatg ctctcccaac acttcttgaa
    57061 ataatcatag ttggcgctat tggaactcct ttagtgataa tatcaatccc gcttaaactt
    57121 ttacctttat catcacgccc gaagatatca gcaaatgcac ctgtttcttg ttggataatt
    57181 acaccatcag gaattttaaa gaagaaatgg attcctttac ctggtgtgat ttccgtataa
    57241 gtagaaggaa atggatcata cattgaagca agttttttat aattctcaac gccgtttcgt
    57301 gtttcagaat gttgatctag gtcaactacc accatttgat gatcttcaag cattaaccca
    57361 atatttctag ttgcatcttc cgtccaccat tcattaattt gattagaatc gttagttgca
    57421 tcattccaac cactagagcc ttttattgga attttagttc caggagataa gggataaatt
    57481 gaccatcccg acttttgtaa ttccgttgct gcttgataat tatttgccaa taaacctctc
    57541 ctcctttttc aaaatactgg tataaatcat tcagacacct ccatgagctt caactgctca
    57601 agttcgtatt tcaacccatc ccgaacaaat tcagcacctt ttaattgatt ctgatacttt
    57661 acccatgtat taaatgtttt cacccttgct tcttgcatat cagtaatgtt taaccatgcg
    57721 gttaaaatct gattttttag ctcgttgatt tctcgacaaa ttattttgtg ttctgcatgt
    57781 tttttgagta catacaaaac tgttagtttt tcattgaatg aatttccaaa gtaggttttc
    57841 ccgatattat tttctagttc agcgatccct tgattaattt cctctaattc aaggctatcg
    57901 agttggttta atagttttgt tttgtacatc tacttatgcc ctccagttct taattgatag
    57961 tcgtactcgt cataagcagt gaagagatat aacgctaatc ctgcaaagat agctattttt
    58021 gcaattaatg gtaataagcc gaaaattccg ccgattaaaa tgcttcctat aattaagttt
    58081 cgtttcataa tggttccctc cgatttttta atatggtata atcggaacaa agaatatcgg
    58141 gttttatccc aattaagttg cctttatcct gtccgccaag actgttatgg ataaggcttt
    58201 ttctttgttc tcgatcatgt taatgctcct aagttgtctg caattcagca gaacgattat
    58261 aaattttctc actcactgaa aactgcaacc catatttttc tttaacagtc attaattcta
    58321 ctgtctcatc taaaataggt tcacgatcaa ccaacatttc aggagtcata tctttctttt
    58381 ttaccatttt ggaatgacca taaatagttg atacagcttt attggctatc acattcgctt
    58441 taatgtagtc tactggtttt ggttcagtaa tagcgtggct taatttagtc attgcttctt
    58501 tttggtgttc tttgtctagc atgcggaata cttgaaagcc ttcaagtcct gtggactggc
    58561 gtagttcttt gatgatgtcg aaaatccagt cttgaaatgc ctcggcttct tttttacggc
    58621 ttttgaatgc catgcggtaa atattttttt tcgttgatga tgtttacatc ttgtgttctg
    58681 cccaatgaat cgatgacctt actagtagta accccatctt taggcaaaga gtgaatggct
    58741 ctagtaacct gtttaagacc taacgcttcc gcaacatctt tcgccactgc ccaccattca
    58801 tcattaatat cgacaaatct tataatgtgt ccgttccaaa tttcagtttt cattcaacgt
    58861 cctccagttc aagccctagc aagctcgcta acaactcaac ttgctgatac aaaatttctt
    58921 gatactcttc aatagaaagt cgttctttaa actctgcttg tactaaatcg ttcaagccac
    58981 gcaatactcc gcttaccgtt ggttcttgtg gtagcaattt ttgatagtcc atttgttcgt
    59041 acaaacattc aaacgtttct tctagcgctt tgccttccaa gtttgctaat tctgccgctg
    59101 tgtcttctgt aagaattgtt gataatactt tttcgattgc tttattttgt gtcgattcca
    59161 ttattatttc ctcctattaa tccctttgcc cccacggctt gcgtttattt ttggtattct
    59221 tccataaagc gatcacattc atcttgtgaa attcttacga ctccatcaac tataatcaac
    59281 tttaaaccgt taggaacata tttctgcatc atagtattgt atgaaacgtt catatactta
    59341 gcagcttgtg ccttgttcat atattttgga aactggtact ttttttctaa taatgaaagt
    59401 aacgttcctt gagtttgctc tacttgttga acaactcgat cgtcaataat tttaaaagct
    59461 ggatcatcaa gcgtgagttt gtcaattttt aattccatat ttatcccccc tcgttagcat
    59521 acatttctat acaaagattt taaaaaaatg caagaggctc ttgaatacat ttctatacct
    59581 cttataaact caatatacca tcataccttt tcatttgcaa gaattttaac atactttttt
    59641 gaacttttat gtttcacaca tatttaaatc gtgatatagt agagaaaata tatgaatggt
    59701 ggtgaaaatg tggctagaaa aaaatacccc gaagcagtgg agacaggaca aagaattaga
    59761 ctggctagaa ttgaaaaggg actctcaatg gaacaacttg gaggtaaatt atccccaccg
    59821 gccagtaaag gtgccgttag taattgggaa aatggctata atcttcccaa taacaccagg
    59881 cttaaacaac tttcaaaggt attagatgta tccactacct atctattaaa cggatcttat
    59941 acattaagag atatgcatat gatgcccgaa attaagcgaa atgtgcttga aaagcaattg
    60001 aaaaaaacta ttgaaaaaaa taactctata attgattcta atctttctaa tgctatcaat
    60061 aacatcgaag aagataacct gactgtctca cagaagattt atttaacaaa cgcaataaaa
    60121 tatcttaaaa caggatctcg aattgaattt cctgacggga gtaccccaat tgcttatctc
    60181 gcagctcttc taaatacgct tttacgatat gactcattaa ttaatgattc agaaaaaaat
    60241 acagatgaga aaaaagagta tcaagaattt tatgaaaatg atcttctaaa agagttcaca
    60301 gctcttgtta ctgagctaaa taaacactat tcaaaataat aataaactgc ctcctccaac
    60361 cgcccccacg gcttgctaca ctatggaggg aaaactatta tggcaacatt caagcaatat
    60421 gaaactaaaa aaggtaaatt ttggctctat gaagcctatc ttggaatgaa caaaatgact
    60481 ggcaaacctg ataaggttcg tagacgaggt tacaaaacaa aaaaagaagc ccagcttgcc
    60541 ttgtcacgct tgcaagtgga ctacgataaa aacggtatta aaaaagcgaa caacgaaacg
    60601 tttaaagaag tttatgactt gtggctagaa acatataaaa ccactgtccg agaagccacc
    60661 ttcatgaaaa ctgaaatcaa gtttagaaag tggattcttc caaaatatgg aaatcttcgt
    60721 gtgaatgaag tcacggtaaa aaaagcccaa caaattgtaa atagctgggc tgaaactact
    60781 gaccaataca aggtacttca ttcaacttct aagcgtattt ttagctacgc tatcaatctt
    60841 gggattattg agactaatcc actagacaac gtattaatgc ctaaaagaac caccactaag
    60901 cataaagaaa aggtcaaagt ttacagtaaa gaacaactga tcaccttgtt tgattaccta
    60961 aacagcaaac ctgaaactta tcgaaacgac tttgataaaa cgttactacg ctttctcttt
    61021 tatagcgggg ttaggatcag tgaagccctt gcattgaatt ggtcagacat tgattttcaa
    61081 gaaaatacgg tcaccatcaa taaaacacta agccaatcca aaaatggcta taaaatcagt
    61141 gatcctaaaa ctgattgtag tgctggggtt cttccactag atgaaagaac aatttctata
    61201 ttaaaaaaat ggcagattaa tcagcggaaa tatatgctga cacttggtgt aactgatccg
    61261 actatgattt tttgtggtat ctataaagaa ctaatcacac accacgctat ttatgcaaga
    61321 ttgattacta tttgtgaaaa agctatggtt ccatttctcg gtgtgcatgt agcaagacac
    61381 actcatgctt caatgctctt ggacgctgga gctactatgg tagaagttca aactagatta
    61441 cggcatacaa aaattagtca gacgatcgat tgttacggtc acttggcaaa agaaacgaaa
    61501 gaaaagactg ttgaaaaatt agtacaacac ttaaatattg actagatggc atacaatatg
    61561 gcatacagta aaaaataaaa aaaggagaaa ccctatcata tcaaggtttc tcctagcttt
    61621 atagtctatt cttcttcttc cgccatgaag ttaagctcat taaagataat tgacgatagt
    61681 tggtatttca acgatttttg aatagaacta atcactttat taagtctaat tcaccttaag
    61741 tggtttttta gtcgtattca aaaacgtatt caagttgcgt ggcgtgcttt tgataagaaa
    61801 tcgtaagaac tagttaatca aacgacttat aaataatgta acaccactga ttaatcagct
    61861 atatccaaaa aaacaagcat tcattcagat acatcaatct ctatagaata caaagctttc
    61921 attatctcaa accattacca caacttggca agtcggttaa ttgaagaaca cctcattctc
    61981 cacttatagc agtggagcaa caagttgagc tattgttgca acagaagcta agaattctgt
    62041 actccattta agttttttta gactatctac cgcaaacctc agcatagttt ttttaggctt
    62101 ttctttaata gcttcatctt taatggtttc gatatactcc tgagcctctt ctacctcttc
    62161 ttcaggtaag gaatcattca aattctccaa aagcaaatca agctttgata ataattccga
    62221 catatcatca atagaaacaa tcatattatt attgtggcct tgctgtatat tagtgttgta
    62281 actatttcca aatatattta ctttattata tattgaattg ttgctcatat agtcctccag
    62341 tcgtcctgct gcattacttg catcttgtaa cgtcacaaaa aaatctctcc catgttggta
    62401 aattgattca acgatttcaa attctggatt tccatattcg cgccattcgt attcttcctt
    62461 aaagtatttt ccgtaactat ggttaaattg cacagaattt ttaccaattt tccttagaag
    62521 ttttatctct ctctcagaaa taaaattttc ctcaaaagct gattcaacta tctgaaaatc
    62581 actaagaaat gttcctaaac agaagattga ttcattaata gtttgttgaa aattccatgg
    62641 tattttgttc caaaaaccat tcttaattaa agcctctttt ttttcaacaa tctgtgattt
    62701 tacattttct gtatatatct tccaattatt gacgtcttcc ttaatgtccg aaatcgattg
    62761 accttcataa gaagtccctc caccaaaaag tccttgaacc attttatctc atcctctcta
    62821 taataattac tctaaatcca atacttgcta caaatgatag catttcgtgt ggtaacatga
    62881 ttgccatcgt tcctgtttta tttactctga cttcaatact tggtttgttg atgaaatttt
    62941 accaattgat tccatcgatc gtttcatgga atctgtattt actattttca ttgattgtag
    63001 agattgattt attccctctg ttgcacttct gataccttta gtatcaaagt tttgcatgat
    63061 tgctcgctga gctgataaag ctgataatgt ttgacctatt gactctatcg acctcttcat
    63121 ggaatctgta tttaatattt ttattgattg tagagattga tttattccct ctgttgccct
    63181 tctgatacct tcagtatcaa agttctgcat aatttcttgc tggactgata atgtttgacc
    63241 tattgactcc atcgactttt tcatggaatc tgtatttaat actttcattg ctgatagaga
    63301 ctctgacaat ttagtaacac ctgctaaatc gatattacta aaataatttc caatatatcc
    63361 ttcaaaatca tctgacatat cactaaggtc ctcaccagca atatctccag ccaatatttt
    63421 ttgaaaagct ttctctagtt gatttgtata tctcttcaat ttctttatta atttttggta
    63481 taaagccatt tctatttctt tgttatggct tacaatattc ctaccttctc tgattaaact
    63541 catgtctgtt tgaatttctt ctagcttaac atccatatat ttactaaata cttcactcca
    63601 caaagaatat gaatcagata tagtgtgact gttatcttga aataacttca ataattgatc
    63661 ctgagatatt tctttatacc ttacaatatt ctcttcattt tcttgtgtaa ctaaaatata
    63721 attttctcca aagagatatt cttcaaaatc atataaatct atccattgta aaagattctt
    63781 tgagtcaaac tttcctattg cttgtatttg ttttcttttt attgacgaaa tgatttcatt
    63841 tggaattgat tcctctgccc agttatcaga aaaatatgcc ggagctaata aatagattaa
    63901 ttttcttaat gcccattcgt aactatgcaa atacggtaat agttttttcg aaaaaaacaa
    63961 cgcatttgta tctaaaaggg tagtgtagtt taattttgat ttacttgctt caatattgac
    64021 cttagctagg aaaatttgaa atatatgtac ctcttttatt cctatcttac cagtcttatt
    64081 atattgaaat actacgtgta acgaatcatt atagttcttt atgtcaaagc caaatttata
    64141 atttttttcc tgctcctgaa atcttaagat agaatcacta atatatggtg acaacacttt
    64201 cagaaaggtt tcttcgatat tttccattct tcctttttta ggataatata aatattcaac
    64261 aacttgctta ctaatatgtc atcaacttct ttcctttcaa caattttaat tttcctatta
    64321 acttgatatt tgctacataa gccacaacta gcaaacagaa agagaacttt ttctttacct
    64381 tgataactgt gttaattact ggtttctttt ttctatttct ttatagtatt gttcttctac
    64441 ttttaatatt tttttaactt ctctttttat gtcagcatac atagatatac tgttgtcctt
    64501 tcggttgaag cagtgataat tctcaccatc aaactcataa aaaagcatat cacttgagct
    64561 tccatctgaa ttaattaact gaaatttggt attgagtagt aattcatttt tctcgtcatt
    64621 caactttatt aaaataggtt tgatctcttt taaataattg aaatatattt cggttattac
    64681 taaattaaat ttcgctactg tataaacata agagatacgt ggggaatctc taaattggtt
    64741 atatatatct ttccttaaaa catctaactc cttttttaat tgtccgttaa aatcaaaatg
    64801 aggcattgct agaatttcat ccaaatcaaa gtacactctt tgttgatgaa tatttactgg
    64861 cagatgacca tgctgagaat aatttctcat atgtaataaa aacctatacg aaaaatgctc
    64921 atcataaggc ttactcagaa ttttattctt aaaagattta aaatcttttt ctgctaaaaa
    64981 gtttttcata caaacttcaa tggattcagt caaagttttg gctgaactaa ttaagtttat
    65041 cgtcaaagca tttatctgat aataatactg gtcttccgtt aatttttcac ctgtactttc
    65101 aattaaatca ttagtaaaaa ttgtgaagtg tttgagcaaa ttagttaaat tatatctaaa
    65161 cacattaaaa agtttatcca actctataat atgacgtgtt aattctacat aatttaaaaa
    65221 tattctttta tcagattctg ttattgtttc taataatgaa tcaatctgcc ttattagctc
    65281 gtcatcttgt aaattactga caaaaaacct actatcactt ctcataatta tattctccta
    65341 atatgatttt ttcaaaataa tcaatatgta acatagaagt atactctatt gttacatacc
    65401 ttatttctta ttatatcagt atttctctgt gacacataag ggtgtaacat aattatttta
    65461 aacgaaatga ctacaagtag tcattgaagc aaatctccat aagacatttg taaaatcttt
    65521 taaaaagaag cgtcgaaaga aggaactttt cttcaacgct tcttttattg actcaatttt
    65581 caatattact tgaacgaata atctgatttc taaactctcg aaaaacttct tgtggttgat
    65641 aatagtctgg atctatttca aatgtttctg agaataattc attgaatttt tctactgaaa
    65701 taatgatttt accattcttc tctcgactgt tttctattat ttctgcttct aattcaaata
    65761 aatctagtga cgaactaaaa tcataaatcc tgtcatattg aataaaaaaa tcaagaaaaa
    65821 gttgctcaaa ctcttcctta tttatcacta atgtttcaaa cggaacaact ttattttctt
    65881 cagccattct tccaatttcc tcactttcat tttcaaaaat cacaaaaatg atattaactt
    65941 aaaagccact aacaaaataa gacttacaaa gtaaagggta atccaacaaa ataaccattc
    66001 gctctacgta ctacgatttg gtagtcctgc ctttcaatcg gtaattcata atagtaattt
    66061 actgtttctt ctttgtcact ttcaatatac tctggattat ctaatatcat agcatactca
    66121 taaagctctt caccatcctt attttcaatc gaccattgtt gtggacgtat tggtttttct
    66181 gtatcatttt taagtgtata ggtcacttgt aatacacgat catgttgtgc aaattcattt
    66241 cttttttcgg tcacttcaat atttttaaat gttagttcaa ccccatctgc ctgataagtt
    66301 tcacctattt ttaaatctat tgttacaatt tttattggct tttcttctgg aaaatgtctg
    66361 gctttaattg ttagataact tccaaaaaga atcaatccta gtagcactaa aagaattcct
    66421 ttaacgagcc actgaaacgg gaagtaaact ttaaacatct tttttctcct ccgttttgta
    66481 tttgtttttt agagaatacg ataacttttg atcaatgtca tcaagctttt caattacttc
    66541 attacttatc attgattcta gttctgaaat tttttgttca aatttaatgc tttcagaatc
    66601 attaaattta ttatttgatg gactagctaa cactggtaaa tttgaaacca taacacgaaa
    66661 catatttgcc ttggaaccaa aaactccttt gttttggttg taaaaattgt tgaatatttc
    66721 tttagtttct tctgaagccg agactttaaa atatggatag ttcatttttt tcttccacct
    66781 ttcttttttt gggagggagt ctgtagggag cttgctccct cagagattta cccaccatct
    66841 gacttgccag aaagtagcct agcgtgagcg gggcggattg actggcaagt agatgggtag
    66901 ctggcgtatg tcctaaaaac tgcttctttc caagcaactc ttcataggca aaaaactgga
    66961 aagtccacta acaagcccat tcagaattcc atttcaaaat tttgttgcct tttttgctga
    67021 ttttcaatca tttgttgtgt gaattcataa tcctttttag cacgatacat ttttacatcg
    67081 tcattcatta tgcgactgat ttgatagtga atctgtttag cagaaggtgt atgagaacaa
    67141 ttttcaaaac cctttgccgg tagaaataac tctttctgat cagtcttttt aatagaaatt
    67201 gttttacttt tcgcactacg taaagtttga ggattcacat ccgtatgaag tttaatcgtg
    67261 tattcttgat catcttctaa agacattgtg tttacaaaat gcttgtctac ctctcgcaag
    67321 aagtcatcat atttaatgaa tttttcatcg ccttcacggt cgctaaattg cttttgtgcg
    67381 atggaatcta attccacaaa aggtacattt aactcaccag ttttgtaatt tactccacta
    67441 attagcgaca taacttgctc tctttccata ttaaaaaaat tttttatatg tgattcttct
    67501 aaattctttt caagttgttc agaaacagat tcaaatgaat atcttttccc taactttctt
    67561 gcactaaaag tacgttcgac atactgactt ttttcttttc tagattgttt atctcttcct
    67621 aaagtaatta actcacctct acgctcatag aaatttacac cttgtttgta aagaacatag
    67681 ccaagtttgt tatttgtttt tgaaaattga taaacttcaa tgttatattc ttttgccaaa
    67741 tgatccgaaa aatcttcaaa agaattcact tcgcattttt ccaacgttgc ttcgatcgca
    67801 ttttgcacaa actcttgata attaattttt ccatatttca tcttagaagg atcgtttgaa
    67861 taaagattgg ttctcttttc taagttttca tagtactcat tattcactga ttcaagacca
    67921 tgttcattga ggacttgatc gttaattttt cgcattagtt ctaaatcttt agggtcaata
    67981 cgcaaccttt tacatgtttc catgttaata ttattaatga caagatgact atgaaaatga
    68041 tctttatcat tgtgacttgc aacaatgatt tgcgaatttg gaaaagcttt ctctgcaaat
    68101 tcgacagcta tttcgtgcat tttatcaata cttaactctt tagaagtact cttatcaaac
    68161 gaatgaataa catgaaacgc ttgtgctata tctttagatt tattcatctt ggtacgtact
    68221 aaattcattt cttgcagtgc ttcctcacta tcatcactac aatttattcc tgagactgca
    68281 acacattttt ctttttttga aggattagct gcatattgta atagcgagga taaatttttc
    68341 ttgcgtcctt tcgatgttat agtagctccc aaagttgttt cacctcatct tttaaacttg
    68401 caataatctg aattgagtga attaaattcc gatcaacttt ttgggaatac tcttctttcg
    68461 tacccggtct agtttgcttt tctatccaat attcgaaata ttctttttct acttctgata
    68521 atttaaaaaa agagggggca ctccgattgg actccaaaaa aatgtcatct ggataactaa
    68581 tcatcaactt ttttgctatg ctattcgcaa gttcatcttt aaaaatgttg taacggctag
    68641 catcaaaact aaaatgtagg ctcttttctt ttttatcttc agattcttct ctaaaatatt
    68701 tagcaatgat atttaatgcc tttaccgctt gattcaaatt gttaccaatt tgatttaact
    68761 gtttggttaa aactacccta tcatcttcag ctaacaacgg cggttcatat ttgagtccct
    68821 ttaaccgctc acggcaaacg tctgctctag aaattcccct ttgttttgca atcaatgtca
    68881 gttcaagata agtcttttta tcgacacgaa aatttaaggc ctcactatgt ttgcgatttt
    68941 tttcgtacat tttttgttta tcatccattg attatcccct cacatttttt tctagagagc
    69001 aacatttgaa atcgctctct atttcatttt tcatctttaa ttcttcttga atcatcagtt
    69061 caatataatt agtgaccgtg attttttcat ttgttgcttt ctttactgat tcacataatt
    69121 cgtcaagttc taagaaatca tgtgtactaa tctgtagaat taattgcatg acaatcacct
    69181 ctttattttt taagtaaact atcgaaaagt ggattaggat cattaagtgt atctctgaat
    69241 aactcgttgt cttcataggt aaaccttcgt cttggttttg atcccttatc attttcacgg
    69301 atttcactat caataatcac tgtttcatga tttgaatcaa attttttcgc tgactttctg
    69361 tcttcaacga tattgatttt ttgtgttttt tctttcattt ctccactatc tttttgaacg
    69421 ttctcccgaa catctactgt tttgaatcct tgactacttg tagaactttg tacattttct
    69481 cgaacatcta ctgtttttgg ttgacttgca ctgctacctt gcacattctc tcggacatcc
    69541 actgatttaa acccttgact acttgtagaa ttttgtacat tttctcgaac atctactgtt
    69601 tttggttgac ttgtactgct actttgcaga ttttctcgaa catcaattgt cttagtacct
    69661 tgagcatttg aagaactttg taaattctct cttacatcaa cattccgtcc aacatgacca
    69721 ttcagtgaac cttgaatatt ttctcggaca tctactgatc ggccaccatg actacttgta
    69781 gaattttgtg gattttcttt gactaatttt tctaatcctg tatttgaatt tttagcttct
    69841 tgaattcttt ctttggtcaa cgcttctttt ccgtcaatat tagcgcctgt ccgcccttct
    69901 acacgtttcc ttaacattat ttcctcgcga ttcattgctt caggcgtgta tttcgcttca
    69961 tgacgcttct gattaattaa ttcttcagca gacttagctt gaccgtgcat agcattttta
    70021 cgttcttcaa tatcttgttg gactttttgc tgaattgcac ttacaggatt attattcgat
    70081 tcagcattat ttttcattgc ttcacgcata cgttcagaac tagacataat actatcggca
    70141 ttttcaacct tagcaccatc ttttctatcc gaaagacgtg ttggcatgga atcagcatat
    70201 tttccagttt catcttcttt atttttagct aacataggtt tgctattttt attaatagcg
    70261 gacacagtac catcatctaa accttcttta aacttactct tggtatcatt agctatatca
    70321 cgcatactct taaatggttt tgttgctctt tcactagcca ttgtagatcc atctgaagct
    70381 aaacctgata atccacgttc atgagcatac ccaagcgctt tccccgtttt attagctttt
    70441 ttagaaagtg attgtccaaa gttgcgttca ggttttcgtt cttccccttc agcttgatcg
    70501 tttccaccat tatttttccc acctttagac cggttcatac cacctgttcc tttacggaat
    70561 agcattgcac ccattccaaa agcagaagca agttgcccat agccttcttt gagtccaata
    70621 tccacaccaa agtatctcat gactgttcca ctgcctttga ttaaaacaaa cacagcacag
    70681 atataagcaa ttgtttttat aaaaatattt gctgagatac cttcgccagt atttaggtaa
    70741 tttatgaaca ttgcaaacat agataaaccg aacccttgaa atccgactgt caaatagcac
    70801 tgtaaaatat ctgacaaaac ttgttttgat cgttgtcctg tttctaagtc agtagcaaat
    70861 actaaaactc cgagtacacg tttaaatgct aactcgagta ttgctgtaat tataatgaaa
    70921 gcactaaaaa catacgctac cgctaaagca atcaatccaa aagaaattcc ccacaggttc
    70981 gcttgatagc gataatatcc tgatttgata ccatcaacaa aggttgataa aaaattatct
    71041 gaaaatttag tagcaacaaa ttcattatca gaattttcaa ctaattcata acgcagatta
    71101 tcagctgttg ggttctcttt ttccatttta tcaattacat tatctgtcaa tacttgcgta
    71161 agatccgttt tccagaaatt ttcttttgtt agattatttc ggtcatttgt tttatcaatt
    71221 gtcgaatatt gatctgtctt attaatataa gctaaatctg tcactccctg ctcaatcaat
    71281 gaccatgcta caccgccatc ttgattattg atactagttg cgtcattata aagagtcttt
    71341 gcaaacatta ttcctgaaag tttttctgtt gatccaatca aagtcggcat taaaagaatt
    71401 aatgctacag acatcactac gttcattcca accgatttta aatccactgt tccttttcca
    71461 gtaatcattt tgtaaccaac aaacatcagt gaaagtatca ttaaaccaat aacaactgta
    71521 cttaagaccg attgataaac tttattcatt cccgtacttt caataaagct aaacaaatct
    71581 aacacttcgg gaatcatatt ttgaatacca tctacaattg caaacatcga ttttataaca
    71641 gaccacccta tccacaatcc aaactgactg taaaaatttc cgttatattt ttttaaatac
    71701 gattgataga caggattatt caaaaattca gtttgacttt ccgtgtcatc aaaccaattg
    71761 aaaatgcttg cttggacaac attaccggtg aaaacaacga taaacattaa aacaagtatg
    71821 ctcaaagcta accagtgtat ttttctactg gcaatctttt ctttaactat tttcattatt
    71881 acctccctca gcccattttc tgagactctc agagagcttc tgagctgttt ttctgtcaat
    71941 tatgatatga gttacacaat tgtcattttc agatcttaaa tcgagaacta tagtgttgaa
    72001 gcaaatagtc tcgattaatt cgagattatc tgtattttga tctagacacc aaacttgatc
    72061 tattaaacga ttttcaatca aaataacccc tcctatattc aaagttctca aatgaagcgg
    72121 agatacccct ccacttcatt tgactcaatt ctattcttat tgcttatcta ttccttcact
    72181 gatttgcaat tcaattagtt ctaacgcttt attttttaga gaagcaaaga tataatcgtc
    72241 tttttgtttg atacggtgat ctgtttttgc tcttcgtatc aagtcacgta aagtcattga
    72301 tagctgttct ctaacataat ctacaaaata cttgttatta cagatttcat agataatttt
    72361 cagttgaatt ttttgttctt tttcagcaga tttaatcgca tatttgaatt ttttatacca
    72421 gtcataaaat tgactgtaat cgttattacc cactaatttc agacagttta caatattatc
    72481 cccccatacc tgataaagcc attctttttc aattaatgat tcaattaata tttcatctaa
    72541 ttttttattt tcaactgaaa gtgaatcaat aaagtcagca ttttgagatt ctttaaaatc
    72601 tttaaaatct ttagaagaat gattaagttc ttgtaaaaat ttttcacctg gaagcgggaa
    72661 tgacttatca ccatcgaatg ttgatttgtc ggaaggtgta tttttttcac ttggactgta
    72721 atgttgattt gtcggaaggt gtaaattttc ctcaatgcac ttttttttgt agaaatctcc
    72781 ttcgctgaat tctggtttca ataaataata aaaatttgga ttattcttcc cagttttttt
    72841 gttaaatcca ttttttttaa tcgtaattaa tttatattct tctagctctc tcaaagcgct
    72901 tattaccgtt ttctttgtta aacaagattt ttcagctatt ttttctatag caaaaatcaa
    72961 aaaaattctt cctttgctgt caacttttga attctctaaa gaatattcat tttgtttttt
    73021 caaatacata taaacttttt ttgtactttg tttcatcttc ttatattttt tatgtttatc
    73081 taaaaaaatc ggatacttca agtatttttc agcatatacg tcatcttttg agtatgcata
    73141 ctgagcaatt aatttatcta tttcttccat ttattcctct tctttctaat aaatgttaaa
    73201 atgtagtcaa ataaagtttt cttagaactt tattgtcgag tgtttcctgt ggtcgccaaa
    73261 ctgtcgggaa cactcctttt aatttttttc aaagtatttt tatattttct atacttcatc
    73321 attattcaaa caaacacttc cttttttcta tcaaaagcac gccacgcaac tttttatact
    73381 tcatattctt tgataaacct tgctaattct tcttttctaa taaattgaca atctccgatt
    73441 atgcttactt ttaaaccttt ctctctgata aattttttta atgtacttct gctacagctt
    73501 aaatacttac aagcttcccc caatctaaaa acttctttgc ttagagatgt ttcaattcga
    73561 acttgatcta ctgcttcttt tgcaactcga tagaaatatc ttcgcaattc ttgatctaaa
    73621 tccgatggaa gaataacttg aatctcagat ttcattttct ttgcccccac taatttgact
    73681 acatgtattc aaattcaact tttcgtcact tttcattaaa aaaaatttga tcaattgtga
    73741 tattttcaat tcctttttct ttcacaagat acttcaaaat aatcatttct tctggagaat
    73801 agtttgtttt accgctttct ttgtttctta ccgcttgtac tgaaatgcca tatttttcag
    73861 caagttcaga ttgtgataat ccacaaaact ttctaaaatc tttaggcttc atgactcctc
    73921 actcctcctt cttttcaact tttcgtcact aaaagaataa catatattta ttcataccgc
    73981 aacgtaaaat tcaactttta tctacttttt catttcatta tatgagaatg tagtgtaaac
    74041 tgtagaatag aatagatgaa ggtggtgaaa ccattttgtt tgataaaaac attaattctg
    74101 aaattggaaa acgaatacaa gaaatacgtg tttctttagg gtatactctt gaagaatttg
    74161 gcgaaaatct gacacctccc gcaagcaaaa gcaatgtttc tagatgggaa aaaggaaaga
    74221 acatacccaa caaagcgcgg ctactaagta ttgctaaact tggtggtgtt tctactgaat
    74281 atctactgac aggtaattct caaaaaacta agaaatatat caagtattta ttcaatgaga
    74341 ttttttttga caacggtacc ttccataaca aattcaaaga tttcggtaaa gacagagata
    74401 ggcttggtct atatgactta tttttagcag tatcgaaaac atatagtcta gataaatcac
    74461 cttctttaaa agataacttt gtagaaatga ttatagatga tgtatataaa aaactcttgc
    74521 aaactgataa atcagatgac caactcatcc tgggtttcat atatatttgt cttgatgaaa
    74581 aactttcaaa cagacagaaa agtcttaaaa gtatcaatta tgaggtattc aattatttag
    74641 gtgttgcatt tggtttaatt gatagcgaaa tcaaatctac aaacgcaggt aacgatgaag
    74701 atttattaaa ttatttaaaa gaattaacat cttttattga tgaaataaaa acaactacat
    74761 tcaacaaaaa aaaatatttt caaaatcaat atgatataga agaataaaga ttacattttc
    74821 ctatcaaaag cacgccacgc aactgatagg aggaaaaaca atggcaacaa ttaaacaata
    74881 tgaaaagaaa gacggaacca aagcttggat gtttcaaaca taccttggaa tcagcccgct
    74941 aactggtaaa gaagtccgta ctacacgtag aaattttaaa acaaaaaaag aagctcaaat
    75001 agagcttaat agactacttg tagactttga agagaatgga cttaaaaggg aaacgaacga
    75061 aactttccaa gaagtgtatg atctctggta tgaaagctat aaaactactg taaaagaagt
    75121 cactcaatta aaaattgaga tcaagtttaa taaatggatt cttccaaaat atgggcagtt
    75181 tagaattaaa gaaattacta caaaacatgt tcagaagata ttgaatcagt gggctaaaac
    75241 aacagatcaa tataaagtcc tacattctac cgtaagccgt attttcaaat acgcaatgac
    75301 tctagggctg atcactaaaa atccctgtga gctcgtaatc atgcccaaca gaaatgttga
    75361 aaaaaagaag gatactgtca aaatttattc acgagatcaa ttagaaacct tatttcaata
    75421 tttggaatcc aaaagaagta cgtatagggc agagtacgat aaaactttac tgcgctttct
    75481 attttttagt ggctgtcgaa tcagtgaggc tttagcctta gactggtcag atattgactt
    75541 tgattcaaac accgtcacta taaacaaaac cttaagccaa acaaaatatg ggtacaaaat
    75601 ttcaagtcca aaaacgagaa atagctatgg ttccctatct ttagacaata aaaccattaa
    75661 ttggctaaag aaatggcaaa tcaatcaaaa aagatatatg ttaaccctcg ggattactga
    75721 tccaacaatg attttttgtg gaatctacaa agaaatcatt actaaacacg ctatctattc
    75781 tcgtatgatc acaatctgca agaattctgg tattccattt ctaggtaacc atgtaaccag
    75841 acacactcat gctagtatgc tgttagagtc gggagtctcc atgaaggaag ttcaagaacg
    75901 ccttagacac tcatcaatca aattaacaat ggatacctat gctcatctaa ctaaagaaac
    75961 caaagaaaaa accgttgaaa agcttgcaga gcacctaaat ttctagcgga acgtattcaa
    76021 tcccgtattc aattcaaaac aaaaaaaagc tgaaccctta tctaccaagg gttcagctat
    76081 catttacagc ttattcttct tcttccgcca tgaatgtatt gaatacttct tcaatcatgt
    76141 cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct tcgttttcga
    76201 tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagca ggatataaaa
    76261 gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagtaggatt tcatataatg
    76321 tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcatgg tcgtgggaat
    76381 ggttatgttc tgtcataaga ttacctcttt tcttttatta ttaaaataat tgcttacctg
    76441 agccgtctaa ataattttgt aagatcatca cagctgctaa tttatcgatg actttttttc
    76501 gtttggctct tgaagtattt gcctgctcta ctagcatacg ttctgcctga acagtggtta
    76561 atcgttcatc ttgataatca actggcaagg agaatagttc tttgatcttt tctccgtatg
    76621 acatggaagc ttctgccctt ggtccgatgg aattgttcat atttttaggt aatccaacta
    76681 caaaccggtc tacttcatat tgtgctacta attccttcaa ccgatcaaag ccaaactctt
    76741 cttctgcttc attgatccga atgatctcta ctccttgagc tgtccagcca aaggggtcac
    76801 tgatagcaac acctactgtc ttagaaccga catccaagcc cattactctc ataaattcac
    76861 tccatgattt gagaggtagt ccttgacaat tacttccatg atctcatcgc gttcgtgacg
    76921 gcgaatcaaa tttcgagcat cacgataacg aggaatgtag gccgggtctc cagaaagcaa
    76981 gtagccgaca atctgattga ttggattgta gcctttttct tccaaagccc gataaacaat
    77041 cgctaatgtt tcgctcactt cttttttgcg actatcgtca aaaccaaaac gtacggtttc
    77101 atctgtaaaa cccatgattc tacacctctt tcccgcaagg taattccagc ttaccttcta
    77161 caaccttatt ttactcgaag aaagggaaaa ctacaaacaa atattccaaa cttccgaata
    77221 aattaagaaa cactatttgt atgttttttt ccttttttct gtagctttat caaaagtact
    77281 cttttcataa agtatacgct atatttcttt gaatgtatgt ttttacacaa ttttttcata
    77341 atattcataa aaaattcgaa tagctgctac taattttttg tttttgttgc ttgtcctgtc
    77401 tcataattga tcgtgtagcc atcttgaaca ttgtagataa agacattgaa ttcaattttt
    77461 ttgtcttcaa tgctttctgc ttccaattgc actcccctgg ctaccagttc attcccctca
    77521 aaatgaggag tgacacggta gcgtacatga tgatttgttt ctttgatata tgccgcaata
    77581 tcattttcgt gagcaagcat gtatggagca ttcaacgaac gtgtccctgt catcaagttt
    77641 ttaatgttgt tattttgacc agtcagctgg aaaccaatta aatggctgcg attatataac
    77701 cattgcccgt cacttaattt cttttgtttc caacctgtcg ggtcaatata caaaggctct
    77761 ctcttttcag tagggaacaa atcttttcca agcatagcat tggcttgtcc tactctgttc
    77821 aatttatcaa tatttgaata gctttcccat gttcctttgg aaaggctaag atcctctttt
    77881 gtaaattccg gctgattccc attgatttct atttcttgat gttttccatc atattctggc
    77941 acatccgcta cttgagaagc ttgtggtgtg gaatcaaaaa tagttgtaat ctgctgaaag
    78001 atatctgaat ctttttgtgt ggtggaaaca ccgaatgccc ctgcaatcac agcagcgacg
    78061 atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta ctaacagctc
    78121 cctaaatctt aacatatgtt cccctataaa taaatactag aaagaaatag aaacagcaaa
    78181 aaataaaacc tgttttaaga tggcttaaaa atacaaaaaa gagacggatt cctcgtctct
    78241 aatcagtcac cactctatct gtaaggtatt tattattatt caaaaatttt cttcatactc
    78301 aagacaatac actgatttca caaccgcttg gtaaacggtg cgcaaaagct tgcatctgcg
    78361 gtaataagtc catccaagtt aaaaacataa gaagttgttt ttgcttggct gttcttatta
    78421 cttgttgcag agcagctttt tcacaaccta ttttaagcgg gtgacgggaa tcgaacccgc
    78481 gacatcagct tgggaagctg ggattttacc actaaactac acccgcaata caaaaacagt
    78541 atacgcctct tttattcctt cctcaagttt ttttattctt tacgcttctg gtatgttttg
    78601 tagtaaaata aaactaatca atcaattatt ggagaagatg ctatgacggg aaaagaatta
    78661 gatctgttgc tggacacttg tttactcgct gggaaaatca tgatggaaag caatgcggag
    78721 atgtatcggg tagaagatac gatgagtcga attgctttag cttctggaaa tttccgtttg
    78781 gtcagctatg tcactcaaac cggtctcttt attggtcttg atcgtacctc gaccatccga
    78841 atggaacaaa ttaccaaccg ttcgattaat ttagaaaaag tagtaaaagt aaatgatctt
    78901 tcacgtaaat atgttgcttc agagctaaca ttagaagaac tttactgcga actgcaagca
    78961 atcgaccgag accgttcttt ctttcctgtc tggttgcaaa tcgttagcgc ggcagtgatc
    79021 agtggaacaa ttatggtgtt atttggcgga aagatgaccg atttacctgt gactttagtc
    79081 attggaggat tgggatacgc agcttatcta tatagtttga aattttttag aatcaaattt
    79141 ttatcggaat ttttatcttc tttattgatt ggcatagccg ctatctacag cgtacgtttt
    79201 ggcttaggaa gcaatcagga tctaatcatt atcggatgtg tgatgccttt agttcctgga
    79261 gttcaaatta cgaacgctat ccgtgatttg ctagctggac attatgtctc aggagtttct
    79321 cgcggaacag aagcgatgat gactgctacg atgatcggtt ttgcgattgc ttttattttc
    79381 cagctttttt attaggaaag gaacacaagt atggggacga tatttattca gttttctttt
    79441 agttttttag cgactgcggc atttgccgtt attacaaatg tacctaggcg ttcattgatt
    79501 tgttgtggtc tttctggtgc ttttggctgg atgatctact ggatctgtgt tcaaataggc
    79561 ggatctgctg cttttggttc attgcttggt tctttaggag tagcattcat cagcgatctt
    79621 ttctctaaac gtttgaaaat gcccgtaacg attttcaata ttcccgggat ggttcctcta
    79681 gtccctggtg gtttagctta tcaagcggtt cgaaatctcg ttactgaatc ctatcgagaa
    79741 gcggctcatt atgcggtcca agcaattatg attgctggag caatcgcttt aggtcttgta
    79801 ttatcagaag tctttaacca taacatccgt aatttcaaag taaagcgtga cgcaatcatg
    79861 atgaaacgaa aaaaacggct ttttaaataa acgaagtgta acaaagaaaa ggttgtgaaa
    79921 gagacgttca gccttgagcg atgacaaaat atctcacaaa cattgcgaca agtaaccgca
    79981 ggagcaataa gaagtaatta tgagtattaa aaaaagtttt gtaaaaatag cttctcacat
    80041 ttttacataa aattgactta atactgagaa actgactttt gaacaccctc tatctgtaaa
    80101 aaaggggctg cgacatgatt tttgtcacgg cccctcttct ttattcaaat tttttccaaa
    80161 acgttcgttg ataataaacg atccatagag cggaaagtat tagaactaaa ccgatcacag
    80221 gaatattcac taaccaaaat ggataaaaca aagccaacat tccgtataag acgataagaa
    80281 taatactgat gatcaatgaa cccatcatag ttgcgactaa acccaaactt cctgaacctc
    80341 tgttaaataa ttggttaata ttggtccagt tcagcagcag caaccgataa tctctagcga
    80401 aataacgtag tgacaataag tagcagccga ataaagaacc aaatagcaag ctagggataa
    80461 aaaacaaagg cagctgaaaa gacagtccgc ttaataatgc gatcccacca gttaaaaaac
    80521 tctgcaatat ccatccgaat ctgaactttt cttttaaata ttggttcata gagattggca
    80581 aagaacggat aaacagaaaa ttttcttgat ccaaagaaat aagattgctg ataaaagaag
    80641 tctgattaac tgttaagaaa gccaatgcga tccctaccag gaaaacaaca ccgattaact
    80701 tattatctaa atcagttaat tttatttcgc ctcctagggc aaaagcaaga ataaaaatga
    80761 ttggagttaa caaggaagaa gaaagaactt gcataatcaa attaggttct tttactaatt
    80821 gagcattgta actaaataaa agctgatgca cattttggtt ctttttatgg gttcttttta
    80881 ttttgaactc actgtttgaa tcagtcagtt gctctgtaag ctttggtact atcagctgtt
    80941 ttacagcaag gaaaaatccc agagccgtga tagctagtat tccccaaccc aataaacctt
    81001 gttgagaaag tggctgactg gcagcatcat agaatggcaa taatatcgaa atcggatgcc
    81061 gatctgttaa ctcattttca atagtccccg tttgatgatt catccacatg attccagcaa
    81121 atgcgatcag catcgagctg ccaagcaaga tactagaaac tgtttttcta tgttttttaa
    81181 acactgctgt tcgagccaaa ccaaaaacaa tcaaactagc cagacaaaaa agaataatca
    81241 aaaatagaga aaacaaaagg attgagataa tgatagtaag caaaatccct agatcagctc
    81301 gccatccggt taaaataaac atgactagaa gcggaaaaac aaaaggaatg atggtcaaag
    81361 caactaccaa aatctttgct gaaaaaacct gttcttgtcc aaaaggcaga ggcaaaaata
    81421 cttgcaggtc atttccttca aaaaagatat tataaatgac tgatatactt tgagttaaac
    81481 ctaacatacc aaaaagtgcc acgtaataag taaagaaacc tggcaattta acaaagttca
    81541 tagcaaacat agcaaatccg taaatcagta aaaaaatcag ccctgaaaaa agattttgag
    81601 acaaaagata gatagttaat cgctttccct tttttccttt ctctcttgct ttattcgtga
    81661 gctgtggatt aacatataaa agattaactc gaattaattc taataattgt cgcttataca
    81721 tacggtccat ctcctgtcgc ttgccttcca gccattttca aataaatctt ttctagcgtt
    81781 tcatcaggtg agccattcaa taattcacta actgccccct gataaatcaa ttcacctttc
    81841 ttcaaaatag ccagtttatc gcataactgt tgtgcggtat ccaatacatg tgtagaaaaa
    81901 atgactgttt ttccttttgc ggcatgagcc ttcatcattt ctttcaaatc aaatgctgct
    81961 tgaggatcaa gtccttgcaa aggttcatcc aatacccaaa tatctggatc tggcagcaac
    82021 gtgccgatca aaatcgtttt ttgacgcatt ccatgtgaaa aacttgcgat tgtttcattt
    82081 tgatgactat acatatcaaa aagcgctgtc aattctgcta aacgcttttc ttttttttgt
    82141 gtgtctaatt cataggcagc tgaaatcaag tcccaatatt ctccagccgt caattgtaaa
    82201 aaaatatctg gggaatcagg cacataaccg attttttgtt tgatactcaa tcgattttcc
    82261 gtcaatttca ttccatctac taaaatcgtt cccgatgatg gttcaataat actaactaaa
    82321 cttttaatcg tagttgattt tccggcacca ttatgtccta aaaatccaaa aatttcccct
    82381 tgtcttatcg agagagacaa gtcttttaat gcttgttttt tcccgtaact tttacttaca
    82441 ttgatgaatt caatcatttc tgtactcctt ttgtttgtta ttacttttta ttgtaccctt
    82501 cgttattggt ggagtaaatg tcaaaaagag tgcataacaa aaaaggatag aaacgactag
    82561 gctgctactt gggttgttct cttctacagg aaaacactct tctaacaggt aagatatgag
    82621 tctttgtttt ctttaaaatg attcattttc tatcttttct tgcataaaat tctttagttt
    82681 atcaatactt ctctttccca tctctagttg ttctccattt gcaaatgtta ctatttcgtt
    82741 tactctgcta atagatgtaa tgttttcagt attaatgata taagctttta aatctttaca
    82801 taaatactca tattcgagtg attttttata ttttttaatt gtgccgttta cgagaatctc
    82861 ttcatctttc aatactaggc ctattttatt tttataacct ggcatgctca ttagataaag
    82921 gatttcatcg taatggatat agtgatcgct attaccataa cggacagttg tccacctttt
    82981 tttcgtagca tgttgaacag gatagttttc tttttctttc aataagtaac tttggatgct
    83041 ttttttattt ttttcataag tgaattcttt tatcaaataa ccactaggcg taatctttcg
    83101 attaataaca gttattgctt tattcggatg agacgttaga taaacaattt tacacttttc
    83161 attcttttct ctaagcattt cgcctagttc gataccgcta taataggcgt tcaaatcgat
    83221 gtctaggaaa aaatatctga ggaatagatt tcattatttt taatgaattc aacaaattgt
    83281 ataacgttca atccctgaac gatctcaaat ttccaaggac ttaataaaat tctggattct
    83341 tccaaaattt ctactagcat tttttcaaaa accaaactat catccaaaat aaatatacgc
    83401 gttaccataa aaagtacctc cactcgatat tttactatca aaatcatttc atacaacttt
    83461 tcaacataca tgttaggaat aaattgtaat tctcttcaca atcttttcca acacctttga
    83521 aaccgatatc aaatactttc aggatgctgc ttctaaaata aatatcaaac aaatgaatac
    83581 atcatgcaaa aaaagccaga caaccgacgt ccttgcgtgt cagctgtctg agctatgttt
    83641 atttattttg ttcttttaaa aagaacgata cacatactga tttttcggag cagagacttt
    83701 ctcagcgttt gtaacaacta cagatgggat ttcacgcttg aatggttgat aatagtctga
    83761 ttggatggtt ccttctattt tataccattc atcattttta accgttactc cttccggcat
    83821 atgcgtcaac aaaccaaaga ctcctgaatc tgccacacaa tggataatgc cgaatcggaa
    83881 caagaagata tctgtattgc tatcgttcgg tgtttggtaa acaaatcctt catagctgat
    83941 ttttttgcca atgaattcgc tagggtaatt ataaattagc tccataactt ctaaatagtt
    84001 ttcatctgtg atcttgatag tttgcttatc cccatacttt tctttcaact cattcatttg
    84061 gtctaagtag tctgatttgt tgaaataaat actggtatcc ggctttaagt attgtgtctg
    84121 catctccgga tcccctactg attctttact tactgggaaa ttaaatcctt tggcttctac
    84181 gatcgtagtg tcgagactaa ctgttggaaa caacgtcccc acgattactg gcaaagctag
    84241 cagtacataa gcgattccac gttgataagg ttttgacaac ccatggtcat gatcgtgctc
    84301 gtgtgccttt tcttctgctt ttccgttttt gacccacaaa tacagctgaa ctaaagccaa
    84361 aagagcagat aaaatcattg aaagaatagc taagtagcga taatggacat tgatatattg
    84421 atcgagtttg ccagaaactt gcaagtacat catcaattcg aaatacccag ataaaatcaa
    84481 aaaccgcagc atattttccc tcctaaataa ataatgtgaa tccaataacg ctaagggcaa
    84541 tcagtccgat cagcgataaa ataaagcgtc ctgtaaaata tcgtttcatc atcagtagat
    84601 ttttgatatc gaccattggg ccaaagacca aaaaggcagc tacaggtgct gtgccgaaca
    84661 gacttaacaa agaagatcct ataaacgcat ctgcttctga gcaaagagac atcgtaaagg
    84721 ccaataacag catgatgata atggctaaaa tctttgttga gccgagcgtt agcataattc
    84781 tagttggtaa ataagtctgc atcgcagctg cgattaatcc gccaatgatc aaataacgac
    84841 ctgtatcaaa aaattcgtcg attgaatgtg tcaacacatg cccagtttga tggatcagct
    84901 gattttttgg ttcattcttc tcactttgtt cttcttcatg gacatgatga tggtgatttt
    84961 ctgctacttt agacgtgatt ttttctttca atacagcgtc tttttgaaaa taggcaagcc
    85021 agacaccaat cacaagggca acaaaaaagc tgcctatcac ccgccagctt gccatctgcc
    85081 aagaattgcc aaatgcgata aaagtggaaa aaatcacgat tggattgatg atcggagcag
    85141 tcagcataaa agcaaacgct gtgtgaactg gtacgccttt tttaacaaat tgatgaacga
    85201 tcggtacgat gccgcattca catgaaggaa agaaaaatcc taagatcgaa ccagtaaaaa
    85261 tagagaagat acgattttca ggaagccatc tcttcacacg ttctggggtc aaaaatactt
    85321 gtaaggcgcc tgagataaga cagcctaata atacgaaagg taatgcttca ataacgatcg
    85381 ataagaaaat cgtactcatc tgcaaaaccg aatttggaat aaaagaaaac atgagtttcc
    85441 tcctttttat ttctagttca ttcgctttgc aaatatacgc ttttattcaa aaaaaacaag
    85501 ttgctctgta aatttttaca caactcttat tttttaccaa agcacttcaa ttgacttttt
    85561 ctgttattcc cattaaatta aggtctcata catatcactt ctatcataaa agggagaata
    85621 aaaatgaaac aaaccactaa cttgactgaa gttcaagaca tactgcaaca gtcatccgtt
    85681 gccgtcattt atctttcgat gcctaattgc agtgtttgtc acgctgttcg tccacgacta
    85741 gaggaacttc tcacacatta tgatattccg gcacttcatt tggatgcatt tgaaacacca
    85801 gaagtagcta gtaaatttga agtgctgact gcgccagttg tcctgatttt tcatcaagga
    85861 aaagaagtct ttcggcaagc acggttcatc gactttaaaa aaatccgtta tctactagat
    85921 gaactgacag cagaagatga ttccctttct tatgaagaaa tcttccgaac aaaataatcg
    85981 ttgtccaaac agaaaaaagc gattgaacaa gtgaaagttt tgccttcatt tgttcaatcg
    86041 ctttttattt ttattctgct gtattttcgg aatgcgcatt tgcgtcagaa tgcaagaaat
    86101 agatcaaggt ttgcaattca ttcgtcaaat caacattttg gatcaataca tctgacggag
    86161 cattcaatcg agcagcggta aagttcaaga ttcctttgat tcctgcttcc gttaactgat
    86221 tgactacttc ttgtgctctt tcagcgggta tcgttaaaat tgctacttcg atttgttgga
    86281 tcttgatttg ttctgacata tcttccattg gataaacggg gataccgtct acgatgcgcc
    86341 caacgatttc ctcattcaca tcaaaagcac aactgatacg tatgctatta ctttgatgga
    86401 atttgtattt tagtaaagca cttcccaagt tccctacacc gatcagcgca acatttgtca
    86461 attgatcgtc gtttaatgtc tttgcaaaaa agtgcataag atcttctaca tcataaccat
    86521 acccacgttt tcctaattcg ccaaaataag aaaaatctcg acggatcgtt gcactatcta
    86581 cttgaacggc ttcacttaat tcagttgaag acactttttt ctttccagca ttatttaaaa
    86641 tccttaaata gcgataatac aaaggcagtc ttttagctgt cgcctttgga ataatatgat
    86701 ctttcacgaa atgacctcca cgtattgtct aattacatct ttgatttctc catcatagca
    86761 gtatttatag gaaaaaatca attccattgc tcagatattg tgaaatttta ttctttattc
    86821 atttatttat catttctgca aatctcgcag tataatggaa tttatggtag actactaatt
    86881 gaaaaatagt tttttatttg aggagaattc acaaagtaga aaagaggatt ccatgatatt
    86941 attacaagcc aatcagattg ctcgccattt tggtgcagaa gtcttatttg aaaacataca
    87001 tttagaaatc agtacaggcg ctcgtgtcgc tttagttggc agaaatggcg ctggaaaatc
    87061 gactttatta aagatcattg ctggaattga agcacctgat gaaggaaaga tcgctaaaaa
    87121 taaaacagcg actctcggat acctcgctca ggacacagga cttgattcca atgaaacagt
    87181 ttggaacgag atgctgaaag cttttgaaca agttagacaa atggaagagc ggatgcgcga
    87241 gctagagatt ttcattagcg aaggtacgcc agacacacct gcttatagtt cttatttaaa
    87301 agaatacgac cggctgcaac acgatttttc agatcgaaac ggctatggtt atgaaaatga
    87361 aattcgttcc gtattgcatg ggtttaaatt cgattcttca ttttatgatc agaaaatcga
    87421 tactttatca ggcggacagc gcacacgttt agctttagct cgcatgcttt tgcaacaccc
    87481 tgacattttg attctagatg aaccaacgaa ccatttagat atcgagacac tttcctggct
    87541 tgagaattat ctgcaaagct atactggtgc attattgatt gtttcccatg accgttattt
    87601 tttagataaa gtagtcaatg aagtctatga attaagcaga agaaaaatgc gccattacaa
    87661 aggtaactat tcgaaatatc tagatttgaa agcagaacag ttagcaagtg aatggaaggc
    87721 ttatgaaaag cagcaaacag aaatccataa attagaagac ttcgttgctc ggaaccttgt
    87781 tcgcgcctct accacaaaac gagcgcaaag ccgcagaaag acattagaaa aaatggaccg
    87841 gctggatcgt cctcaagggg atgaaaaaca agcaaacttt ctattcagta tagaaaagcc
    87901 ttctggaaat gtagtcttgc aaatcgaaga tgccgctatc ggatatgata atgtcacctt
    87961 atctgaacca atcaatctag atatccgtcg tcaagaagcg attgctctag taggtccgaa
    88021 tggtatcggg aaatcgacgt tgctgaaatc aatcattcac cagatccctt ttatccgtgg
    88081 aaaagaaacg ctagggacaa atgtcaccat cggctattac gatcaaggtc aagctgatct
    88141 gcatacaaac aagacgatcc tagcagaatt gtgggatgag catcctacca ctccagaaaa
    88201 agagatccga agtgctttag gtgctttctt attttctggc gaagatgtag aaaaaccaat
    88261 ccctctgcta agtggcggtg aaaaagcacg ggttgcttta gccaagttag cgatgaacaa
    88321 agaaaatttt ttgattttgg atgaaccaac aaaccattta gatattgaca acaaagaagt
    88381 attagaaaat gcattgatcg attatgacgg cacactgctt tttgtttccc atgaccgata
    88441 tttcatcaat cggatcgcga caaaagtcgt cgaactctct gaaaatggca gcaaagtcta
    88501 tctaggagac tatgattatt atttagagaa aaagcaggaa gaagaagaaa tcgccgaatt
    88561 gttggcacaa gatgagccag aatctgctcc tgctcccacg aacaaatctg attattacca
    88621 aagcaaagaa cagcaaaagc ttatccgtag ccttcaacga aaaattactc aggttgaaga
    88681 agaaatggcg cggatcgatg aatggatcga tcagttggaa caagaaatgg tcgcgccaga
    88741 aaacctggaa gatcacgtaa aactcaatga gctgaaccaa gatttagaag ctgctagaca
    88801 agatcaagaa acaaaattga cggaatggga agaactaagc ttggaattag aagaatatga
    88861 aaattaacga aaaaaccaaa acagaaacca ttcatgctgt tttggttttt ctttttttat
    88921 tttatcaaac aaaaattcca tatttacctt tttcaccaat aagaagacca attactagtc
    88981 attctcacta aagcttctaa ataatagtaa tcaccccaaa tacaaggtgt atttacccca
    89041 ttatttttgg gcttattata aacagactgt gttaaaaagc catcttcttc ttccgttgta
    89101 gcatatcttt ctttcaattg tcgcataatt ttccaaacag cagactggta taagttcttt
    89161 tctgttttgt ctaatagacc tgattctatt aattctaata atccacaaac catgatcacc
    89221 atactagatg tatctttttc ttctctgcta gaatcatcaa agatcaaatc ccaataacaa
    89281 atggtatctt ctggcaaccg attcataaaa taattggcta atattttgct taaatcaaaa
    89341 tatttttgtt ctttcaaata acgataactt aatgcaaaac cgtaaatccc ccatgcttgg
    89401 cctctagccc aacaggaatt atcactataa ccttgatgcg tacttccata tcttggtttt
    89461 ccatctttga catccatata aaatgtatga taagttgaac catcttctct tactaaataa
    89521 tctgctgcat tttgaatatg agcctgagca gcttttttat atttttgatt tccagtttct
    89581 tcactcgccc aatacaataa tggaagattc atgcaacaat ccatgatcat tctaccttgt
    89641 tgtgaaggat cactaggatc tccccaagct tgtataactc ctgccttctc ccaaaaacgc
    89701 tgaagtaatg tttctgcagc atcaattgaa gcttccttca tttttcgatt tcccgttatt
    89761 ttataatctg ctacggttga taaagaatac aaaaaaccaa gatcgtgatg attagtgata
    89821 atttgacagt caatcctgtg gataaaatct atatctgctt tttcagctgt ttgttgaaat
    89881 aaaggatcac ccgtcttctc aaataacaac caattaattc ctgtccaaaa tccttctgtc
    89941 caaccaatat tgtctcttaa tggataacgt ccttttatag gacactcgct tgggaattga
    90001 tactgactgt tactgatacg tttgattaac ctctccgtgt gtctgcgaca acgctcgatc
    90061 tcattattcc attctttttc cattaattct ttataacatt catttatcca tttttctgga
    90121 tgtattatct cttcaaacat cactttatca agcatttccc caccagcctt tagcataatt
    90181 ttagtctttt tttattgttt ttattttgga tagttagccc ggttgtccca tcaaaatcaa
    90241 ttttgggacg attgttccat acttctaaac tataatcccg atcttgatga gcaaccactc
    90301 ctgttccgag ccaatgggtt ccttttccaa atgtaccctc taatccagga ataaaagtgt
    90361 tttcccatgt catcaagttt gtattgggca aacccataat agctgtggat tttcgtttaa
    90421 tagtatcgtc acttaaacag ataatacctg aaaagccagc ttcactcacc ctatagtttt
    90481 ccttgtcttc ttctatgatt attccttctt tctcaggaga acattcaata gcaaaccctg
    90541 tctcatatag aacatattct gtatctaatt ctactttgtg tacgcgaatc tgccagcctt
    90601 caaaaggaat aagccaagtt ctgatttttg cctgtaaagt aggttcccat gtagaggcaa
    90661 gtacctcttt cttacataca atgtctcgta cattatttcg aacgatgaac tgtccatagg
    90721 ttttgatgct ctcattctta aaactggtcg tgatatatgt gtccgctcgc tgaatcccta
    90781 gcattgaatc cattgccatt tctgctttca attgattcgc tcgcggtatc gaaaaaccaa
    90841 attgactaga ataagcaaat tttaaatatt tttcattggt caatgtatgg aaatttggcc
    90901 ctaattgtcc cgcattcaaa aaaaatgtat gtccattatc atgttgtatg agcaaattgg
    90961 cttctgaaag aagtttagtt ccttcacgaa taggcatagg catttcttta gccgtccaat
    91021 actcatggtc ttcaggtagc gccaacaata aaaaaatctt attcagccat aatggagata
    91081 acgtactatt atagggttca gtcaatgtca attgagggta ggtataacca atattcagga
    91141 tacctttttc atcaaatatt tctttttgca tccaccatct tatattccga ttgactaacc
    91201 ccttgatttc acctaattta aaaggcaaaa gatcggccca caacatagct gaccaaaagc
    91261 ttgaaactgc aaaacgataa atcatgcttc gaccaaaagg cacatttgca ccatcttccg
    91321 caaaaaaatg aatatagtct ctagaaaaat caatagctcg gttcttatat atctggctaa
    91381 tgggcttttc tggttctagt acgctataaa tcaaaccata ataatgaaat gcaaaaggat
    91441 tataataatc ttggcgacct cttgaactgt ctcgatacca gccattttga agataacaat
    91501 tttctatatt atcaaatgct tctttcagtg ctgattcttt aactggataa ccaaattttt
    91561 ggataattgt tccgacaatt atcttaaaaa attgccaatt accatctgca cattcatatt
    91621 caaaaatttg cgagaaccaa tgaataatct ggcttttttc tcctgtatct aattttttcc
    91681 ataataattg ttcatgatga tataacgcta ataacattgc gggcatttct accatacgct
    91741 ggtctctttc atgtatttct ccccaataat cgggatggga aggatctgtt ccagctttta
    91801 ttcctgatag aattaatttc aaccatcgtt catctaattg gtcaattgcg ggccccgctc
    91861 cccataaaac ccgagagaat ccttccattc ctgcaatttt atttccatat cctacccctt
    91921 gatgtatata atcaatttgt gcgccacttg ctcgataaaa cggctggagt ggttgattta
    91981 tctttttaaa taagtcaaag taatcctgtc tggtccttaa tagtggtact ggatgtttca
    92041 ttgtattttc tcctcttttt actaccaaat agctaattcc gactgttcta atcgaagtaa
    92101 gccttcaata aaaaaataat cagaatagat cagtggcaca ttataagtca gttctctatg
    92161 gtatgcttct gctccgcctc caatgatccc atcttcatcc atattccaat ttgcatagtg
    92221 cttttctgtc ttttggagta tattgatagc agcttcatga tacatttttg atcgtacaga
    92281 ggaaacatat cctgctaatt ccagaagacc gcatgcagcg catactccag cactggtatc
    92341 aatattcgaa tcttttacag caaaatcaat gagaggaata taaccagttt tttcgatttt
    92401 ctgaataaaa tttactgcta tcttttctgc tatagctaga tagtacggat tttttgtgta
    92461 acgataactt aacgcgaatc catataaagc ccatgcattt cctctgctcc aacaagaatt
    92521 ctcattgtat ccttgcccgc caagtaaccg aatatatttc cctgattctg catcaaattc
    92581 tgcgatatgc ccggttgatc catctttcct cactagatac ttagctgttg tatctgcatg
    92641 ctgccgagca attttagaga acctcggatc accagtttcc ctcgaagccc aatacagtaa
    92701 tggtaaattc atcatactat caataatcat ccaaccaggt ttatggtcat tccatgctac
    92761 taaatagttc cctgaaatat tatagcgtcc agcaagtacc gtagctgcat gtaaccctag
    92821 ttgtcgacta tgtttgtttt gagactgttg ataatgtgca atagccgatg gtaagaataa
    92881 aaaacctaca tcatgatgaa cgtctgaaaa atggtacagg gcagattcta ataattgttc
    92941 ttgttgtatt gctgcctctt tgaacttttc tcgttttgtc ttgtgaaaga gctgccataa
    93001 aataccactc caaaaaccat tggtccacca gtcgattcct ctgtccttta ttacgtctgt
    93061 atattttccg ttgtaagaaa tatatggaat tttattcccc attctgtcta attccgcatc
    93121 tatcttttca gaagctacat tcaaaatttt tcttgaccat tccttcgtct gttcattcat
    93181 ggatatcccg ccattctttt aatttttatg aatgtggagt tgctcctaga acgaactaga
    93241 tggttaacat tcgttatttt ttttacttat ttgcagctcc aatgtcattc tccttaaatt
    93301 tagactgcgc ccacacataa atcaagacca ttgggatcac tgaagttgct aaaattagca
    93361 taattgcgcc ataattaatt ggctcacctg tactagaaac cgtcatattt ttgataatta
    93421 agggaaccgt atatttatct ttatctgaaa gcatgactaa tggaaataga tattgattcc
    93481 aacttcccat aaagataagt aaaccactag taataatcgt aggtgataaa atcggtacag
    93541 cgattttcgt aaaaatcacc cactcattgg caccatcgat tctggcagcc tccatcagtg
    93601 ctgtaggaaa ttgactgcta tattgtcgca tcataaacac tgaagttgta ctggcaatcg
    93661 aagggatgat cacacctgca taagtattcg ataatcctaa acgactcata atgtaaaata
    93721 aaggaatcaa tgtggtaaaa aaaggcacca tcctagatag cataactgta ttgaacaaca
    93781 gtttattccc cataaaacga tactttgcta atgcataacc cgccattgta gaactggctg
    93841 tagctaaaac tgtactgatt acagcaataa caatcgaatt ccaaataatc aagggataat
    93901 tatatctcgc catgatggct tgccagtttt ttaatagcat atccccaaac ttgaaattga
    93961 ttccgccact taaaatttct tgattacttt gcgttgaagc tacaatcata tataaatatg
    94021 gcatcaaaaa tattaaggca ccaaacacaa taatgacgta cgtaaaaatt ctgctttgta
    94081 tcttttgttt agtcattttt atcacctccg aatttaaatt gaatagccga taatataaca
    94141 gatacaaaca ccatcgtcca ggcaatagca gaggcttttc caaaatcaac catttcaaaa
    94201 cttgttttat ataaattgac tcctacgacc aaagctgaat tttctggtcc attggaagtc
    94261 ccaaagaata tatttgggat attgaaatta cccaagccat tgatagtggc taaaactgtg
    94321 gccattaaaa tgattggctt tagcatgggg atcgttaata aaaagaattg ccgaaatttc
    94381 gatgctccat ctatttcagc actttcgtat atctctttag aaatcccttg aagaccagct
    94441 aaaaataaaa tagtgttata tcctacacta agccaaatca tcgtgatgat cattgatatt
    94501 cgtatccaaa aaggatttgt taaccactgt acaccgttaa ttcctagcgc attcaaaaat
    94561 ccattgaata acccagtcgg attgaaaaaa gtcatgaata cggaagctac agccaccgtt
    94621 gatgtaatgg ttggaatata atagacagtt ctaaaaaatc cttttccttt taactgtgct
    94681 gaattcaata aaacagcaaa gtaaatagct aatagcatcg ttattggaat tgatcctgcc
    94741 atgataatca aaacattcgt gtaagattta ataaacaatg gatctcggaa gatagctaga
    94801 taattgtcaa atccaacaaa ttcaaatgag tagccaattt tcataaaact aaaagcaaac
    94861 gagataatga tgggaatcaa aatcgtcaca ataaagaaaa taacaaaagg tgtgaccaat
    94921 ccataagcac tttttttatg agacgtatct gttgcttttt tcttgctgtt ctttattgtc
    94981 ttgcttggta acgaagattg tatcatcgtt aagtcctcct aatatttttt aatcaccagt
    95041 taattttacg ccgtacttat gagcaaaatt ttctgcttct tttagatatg aattttcaaa
    95101 atcacctgtt tgccaatagt tatatgcagc ggtagtcaaa taagacgtag catctgaata
    95161 agtggctggt aaaacagtat ttgatgtatt ttcattgatt ttattcaata gttcatagta
    95221 tttttgtcct ccttagtagt caaacttttt ctcagctgct tctgtttgat aggccaatga
    95281 gttagatgta gccacgccca gctccagtgc gttggctaag cattcttcat ctgtcagcat
    95341 aaatgtaaga aattgttgtg ccacttgtgg atttttggca tctttaggga cataaaaact
    95401 tgatcctcct gatactgcac cttttcctgg attttctttt gaaaaaggaa tcagtggagc
    95461 catgcgccat ttgtcagatg catcaggaaa attcaaagac atattcgtag cataccagcc
    95521 gccagcaaca aacattgcag attcttgaaa tgctttctca gcatcttgtg caccgtagaa
    95581 atgtacaata tctgagtcga tcatttcttt gattaacttt gcagcatgtt tcgcttcttt
    95641 agtattcaga cggatatttc cttcttcatc aagcaaagat tgattctgct gggctagcat
    95701 gttgataaac aattcttggt ccccagattc tggcaaggca attccaaaac taccagtttt
    95761 ttcttttaat gcttttgcca cctggatata ttcttcccaa gaactgattt cttcaaaatc
    95821 tacacctatt tcagcaaatt tttttggttg ataataacaa agtgctgggg taaaatcact
    95881 agcccaagga ataatcgttt cattactcgt ttgttgacgc aaaatattca atttttgatc
    95941 ataaaactga tcgccatatt tgtcataaaa tccatattca tcagcactat aaaaagcatc
    96001 ttgaaatttt tccagcacac ctgctgctga agtgtctgcc atggagataa tatccggcat
    96061 atcttcgtgt gttaccaatt ttggggtgat catttgtact gttgctgttc ccgaagctac
    96121 attcacaatt tttaagtcaa tatttgctcc agttttgcgg ttatagattt ttgcagcatt
    96181 ttcaacatag tttgtcagtt tgccgtttgc gacatatact tctaatgtag tggctttttt
    96241 tatcattgga tctgcgatgt ttgctgttac ttcttcgtta gtttttccac atgcacttaa
    96301 gctgaataat agggtaatgt ttagcaaagc tatcaccaat ttgtatcttt ttttcatgtc
    96361 tatcccttct ttcttttcaa gttcttatat aaaacgcttt cacttatgta tattaacgga
    96421 attagtaaca caaatattta tataaaaaaa tattttactt gtcgttttgg cttttcattt
    96481 ttgttataat agtcattaat tataaataga aagcttttta tataaaaaga gaaaggaaag
    96541 tggttctttt gttaacaaac aaccaatcct ccaatcttgt caaaaaaaca ctaacctatt
    96601 ccatagatac ccatcttcat ttatctgctg ctccctattt tcgactacta ataaatgaaa
    96661 gcgctaattt atatgctgaa tttgcacata aatctataga actagataat ccttcccttt
    96721 tattcattga taaacagcaa gaagtccaaa tcttttctac tcaaaaggaa acacaaactt
    96781 ttatcattat ccagttttat tttgatgagc aattattagc acatattacg ggacaatctt
    96841 tttctataga tcaagaaata acatctatca ttacttttct gaaaaatgaa cagttcaaaa
    96901 ttgattatct aaaacatagt aagccagcta tttcctatga tcaggaaatc aattcttttt
    96961 tcatactatg ccaacagatg agcattcatt tgctttcctt attagttact tattgtctgc
    97021 aaaaaattca attgtcttcc ttaccaaggc attatcaaca ggaacttgct ttattgacaa
    97081 taccttcaaa tgagaaagtc attcctaaaa aaaagcatat tctttattca gatgaattag
    97141 ttgatccaat catacgatat ttagaagaac atctttcaga aaattttttg cttgaacaat
    97201 tagcccaaat tttttttatc agtccagcca gcttaaaaaa acattttaga aaagtgaatg
    97261 gaaaaagtgt tattagctat tttaaagaac taaaaatgga aaaagcaaaa cgtctcgtgc
    97321 tagaaaatga attaacctat acagaaattg cagatatatt aggatttcat tcgatccatc
    97381 atttttcttc agcttttaaa aaatatacag ggttatcccc ttcaaagtat tatctatcct
    97441 taaaatagaa aaaaattttt aggttttatc cacaaattat ttctctatat tttctgtcta
    97501 ttcaaaataa acaataattt tccggcacaa ttatttgctg ttttttacca taacatagta
    97561 cgatcaacgt agaaaaggag atgtttttat gaaagttgtt gtcattggcg gtgtagctgg
    97621 cggtccttca tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta
    97681 tgaacgcgga gaaaatattt cttatgcaag ttgtgctttg ccttattatt taggaggtgt
    97741 gatcactgac cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa
    97801 catcgatgtc tttactaaac acgaagtaac agcaatcgat ccttctacta agcgattaac
    97861 agttaaagac ctatccacaa atgaagaaat aaaaacagat tacgataagt tgatcatctc
    97921 ttctggtgct agaccagatt atccggatat tcccggagtt tttgaagcag aaaacggctt
    97981 tgtcctccgt agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag aaaaaaatcc
    98041 acaacatgtc gtcattcttg gtgcaggtgt tatgggtctg gaattagctg agaatttcaa
    98101 gcatcgcggc ttagacgtga ctttaatcga tcaattgcca caagtcgctt tcccttatga
    98161 tccagaaatt gctaatttag tttatgacaa attgctgaaa gaaggattag ccgttcattt
    98221 agaaacaaga gttactgaga tccgtgataa aggtcgagaa atagtattat cagatggctc
    98281 cgtcctttct gctgatatgc taatttttgc tgttggtgtt tctccgaaga atgaagtggt
    98341 gaaagcaacc ggcatacaat tatcagatac aggtcagatc attgttgatg accagttaca
    98401 aaccaatctg ccggacatct atgcgattgg cgatattatc gaaacgacta gtgtagtgac
    98461 tggtcagccg atccaaagta tgctttccag tgcggccaat cgtcaaggac atatgctggc
    98521 agatatttta aatggtacgc ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa
    98581 aatctttgat catacagcaa gttatgcggg aatgacagaa catgaattaa aagcatcagg
    98641 cataacaaat tataaaactg tttttatcac tccctttgac catgcctatt tctatccaga
    98701 agctacaaga ttaaatctaa agctgatttt tgatgcagat agcggtcgta ttttaggtgg
    98761 acaagcattt ggagaaaaag gtgtcgataa acggatggga gaactttctg tagcaatcac
    98821 cggaaacttg acagtctttg atttgcctga tttggagttg ccttactctc caccgtattc
    98881 tactacccgt gacccgttga atatagctgg ttatgtcgca atcaatcaaa tgacgaatat
    98941 cgtagaaacg atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt
    99001 agatatacgt gaacctaata aagcaccttc tggcagtatc tcggcaacaa aaaatattcc
    99061 aatgaatgag ttacgagatc gaatcaatga aatcccaaaa gataaaaaag tttacattac
    99121 tttcagaaga ggattgaata cttatacttc tgcccgaatc ttggcaggtt tgggtatcaa
    99181 agcgattttg attgaagaat aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta
    99241 tttttacttg aggagtaaaa atgaaaaagt atatagggtt gttggttggt atgtatatag
    99301 aatagaagat aaatgtgaag aaattgtgca ttatttcttc acatttatta ggttatagtt
    99361 taagaaagtt taaacattgc gcaatttaat tattttagct gtttcttttc ataaaaaaac
    99421 atgttgtgac tctgagacaa atcatagtcc atagtgatgt ctctagagta actacgccta
    99481 atattacgag gcatcatatt aatctatcag cactagtacc taaactagca tccctttcga
    99541 atattttttc ttatataata aagtttgctt tcactcctct aatctcacca aatcgactgg
    99601 cttgcgttgt gtgatactag gaactgattg gtttagaacg acttttctat tgtaatcata
    99661 tttttcgcca tcctcgttgt cttctaatcc ttcaatgaat gatccctaat gtgtgtatta
    99721 aataatttaa tttttttgtt cctatttatt taa
//
LOCUS       DASHFF010000003        96409 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.003, whole genome shotgun sequence.
ACCESSION   DASHFF010000003 DASHFF010000000
VERSION     DASHFF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 96409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.003"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            46..480
                     /locus_tag="U2I75_000207"
     CDS             46..480
                     /locus_tag="U2I75_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1695372.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            581..1672
                     /locus_tag="U2I75_000208"
     CDS             581..1672
                     /locus_tag="U2I75_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN1695373.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            1702..2379
                     /locus_tag="U2I75_000209"
     CDS             1702..2379
                     /locus_tag="U2I75_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN1695374.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2711..4006
                     /locus_tag="U2I75_000210"
     CDS             2711..4006
                     /locus_tag="U2I75_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1695375.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            3996..5456
                     /locus_tag="U2I75_000211"
     CDS             3996..5456
                     /locus_tag="U2I75_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1695376.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            5592..7088
                     /gene="glpK"
                     /locus_tag="U2I75_000212"
     CDS             5592..7088
                     /gene="glpK"
                     /locus_tag="U2I75_000212"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN1695377.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            7102..8925
                     /gene="glpO"
                     /locus_tag="U2I75_000213"
     CDS             7102..8925
                     /gene="glpO"
                     /locus_tag="U2I75_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN1695378.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            8926..9642
                     /locus_tag="U2I75_000214"
     CDS             8926..9642
                     /locus_tag="U2I75_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1695379.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            9948..11877
                     /locus_tag="U2I75_000215"
                     /pseudo
     CDS             9948..11877
                     /locus_tag="U2I75_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            12003..12557
                     /locus_tag="U2I75_000216"
     CDS             12003..12557
                     /locus_tag="U2I75_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN1695380.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(13287..13601)
                     /locus_tag="U2I75_000217"
     CDS             complement(13287..13601)
                     /locus_tag="U2I75_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN1695381.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(13615..13875)
                     /locus_tag="U2I75_000218"
     CDS             complement(13615..13875)
                     /locus_tag="U2I75_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN1695382.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14045..14359
                     /locus_tag="U2I75_000219"
     CDS             14045..14359
                     /locus_tag="U2I75_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1695383.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14361..16016
                     /locus_tag="U2I75_000220"
     CDS             14361..16016
                     /locus_tag="U2I75_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1695384.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            16099..18234
                     /locus_tag="U2I75_000221"
     CDS             16099..18234
                     /locus_tag="U2I75_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1695385.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            18409..18918
                     /locus_tag="U2I75_000222"
     CDS             18409..18918
                     /locus_tag="U2I75_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695386.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            18915..20219
                     /locus_tag="U2I75_000223"
     CDS             18915..20219
                     /locus_tag="U2I75_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1695387.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            20315..21184
                     /locus_tag="U2I75_000224"
     CDS             20315..21184
                     /locus_tag="U2I75_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1695388.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(21296..21616)
                     /locus_tag="U2I75_000225"
     CDS             complement(21296..21616)
                     /locus_tag="U2I75_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN1695389.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            21719..22165
                     /gene="dhaM"
                     /locus_tag="U2I75_000226"
     CDS             21719..22165
                     /gene="dhaM"
                     /locus_tag="U2I75_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN1695390.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            22140..23150
                     /gene="dhaK"
                     /locus_tag="U2I75_000227"
     CDS             22140..23150
                     /gene="dhaK"
                     /locus_tag="U2I75_000227"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN1695391.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            23178..23783
                     /gene="dhaL"
                     /locus_tag="U2I75_000228"
     CDS             23178..23783
                     /gene="dhaL"
                     /locus_tag="U2I75_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN1695392.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            23930..24595
                     /locus_tag="U2I75_000229"
     CDS             23930..24595
                     /locus_tag="U2I75_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN1695393.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            24878..25537
                     /locus_tag="U2I75_000230"
     CDS             24878..25537
                     /locus_tag="U2I75_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1695394.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(25614..27224)
                     /locus_tag="U2I75_000231"
     CDS             complement(25614..27224)
                     /locus_tag="U2I75_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN1695395.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(27237..28118)
                     /locus_tag="U2I75_000232"
     CDS             complement(27237..28118)
                     /locus_tag="U2I75_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695396.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            28373..28972
                     /locus_tag="U2I75_000233"
     CDS             28373..28972
                     /locus_tag="U2I75_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1695397.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            complement(29041..30342)
                     /locus_tag="U2I75_000234"
     CDS             complement(29041..30342)
                     /locus_tag="U2I75_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1695398.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     misc_binding    30605..30810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            30922..32178
                     /locus_tag="U2I75_000235"
     CDS             30922..32178
                     /locus_tag="U2I75_000235"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN1695399.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            32469..34346
                     /gene="tdc"
                     /locus_tag="U2I75_000236"
     CDS             32469..34346
                     /gene="tdc"
                     /locus_tag="U2I75_000236"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1695400.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            34589..36010
                     /locus_tag="U2I75_000237"
     CDS             34589..36010
                     /locus_tag="U2I75_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1695401.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            36119..37486
                     /gene="nhaC"
                     /locus_tag="U2I75_000238"
     CDS             36119..37486
                     /gene="nhaC"
                     /locus_tag="U2I75_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1695402.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(37767..38786)
                     /locus_tag="U2I75_000239"
     CDS             complement(37767..38786)
                     /locus_tag="U2I75_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1695403.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(38808..39815)
                     /locus_tag="U2I75_000240"
     CDS             complement(38808..39815)
                     /locus_tag="U2I75_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1695404.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(39808..40473)
                     /gene="pgmB"
                     /locus_tag="U2I75_000241"
     CDS             complement(39808..40473)
                     /gene="pgmB"
                     /locus_tag="U2I75_000241"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1695405.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(40466..42760)
                     /locus_tag="U2I75_000242"
     CDS             complement(40466..42760)
                     /locus_tag="U2I75_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1695406.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            43020..45185
                     /locus_tag="U2I75_000243"
     CDS             43020..45185
                     /locus_tag="U2I75_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1695407.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45489..46322
                     /locus_tag="U2I75_000244"
     CDS             45489..46322
                     /locus_tag="U2I75_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN1695408.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(46424..48124)
                     /locus_tag="U2I75_000245"
     CDS             complement(46424..48124)
                     /locus_tag="U2I75_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695409.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPAAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA
                     "
     gene            48631..49791
                     /locus_tag="U2I75_000246"
     CDS             48631..49791
                     /locus_tag="U2I75_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1695410.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49807..50250
                     /locus_tag="U2I75_000247"
     CDS             49807..50250
                     /locus_tag="U2I75_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1695411.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(50733..51263)
                     /locus_tag="U2I75_000248"
     CDS             complement(50733..51263)
                     /locus_tag="U2I75_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN1695412.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            51368..51919
                     /locus_tag="U2I75_000249"
     CDS             51368..51919
                     /locus_tag="U2I75_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1695413.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(52072..52545)
                     /locus_tag="U2I75_000250"
     CDS             complement(52072..52545)
                     /locus_tag="U2I75_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1695414.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            52683..54155
                     /locus_tag="U2I75_000251"
     CDS             52683..54155
                     /locus_tag="U2I75_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1695415.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            54152..54862
                     /locus_tag="U2I75_000252"
     CDS             54152..54862
                     /locus_tag="U2I75_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN1695416.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            55094..55378
                     /locus_tag="U2I75_000253"
     CDS             55094..55378
                     /locus_tag="U2I75_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN1695417.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(55659..56078)
                     /locus_tag="U2I75_000254"
     CDS             complement(55659..56078)
                     /locus_tag="U2I75_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN1695418.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(56080..56664)
                     /locus_tag="U2I75_000255"
     CDS             complement(56080..56664)
                     /locus_tag="U2I75_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN1695419.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(56711..58186)
                     /locus_tag="U2I75_000256"
     CDS             complement(56711..58186)
                     /locus_tag="U2I75_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN1695420.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            58367..58678
                     /locus_tag="U2I75_000257"
     CDS             58367..58678
                     /locus_tag="U2I75_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695421.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(58794..61130)
                     /locus_tag="U2I75_000258"
     CDS             complement(58794..61130)
                     /locus_tag="U2I75_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1695422.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            61272..61940
                     /locus_tag="U2I75_000259"
     CDS             61272..61940
                     /locus_tag="U2I75_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1695423.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            61943..62986
                     /locus_tag="U2I75_000260"
     CDS             61943..62986
                     /locus_tag="U2I75_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1695424.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(63223..64104)
                     /locus_tag="U2I75_000261"
     CDS             complement(63223..64104)
                     /locus_tag="U2I75_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1695425.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            64263..64991
                     /locus_tag="U2I75_000262"
     CDS             64263..64991
                     /locus_tag="U2I75_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN1695426.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            65147..65464
                     /locus_tag="U2I75_000263"
     CDS             65147..65464
                     /locus_tag="U2I75_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1695427.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            65457..66065
                     /locus_tag="U2I75_000264"
     CDS             65457..66065
                     /locus_tag="U2I75_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN1695428.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            66120..66911
                     /locus_tag="U2I75_000265"
     CDS             66120..66911
                     /locus_tag="U2I75_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN1695429.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            67049..67537
                     /locus_tag="U2I75_000266"
     CDS             67049..67537
                     /locus_tag="U2I75_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN1695430.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            67588..68460
                     /locus_tag="U2I75_000267"
     CDS             67588..68460
                     /locus_tag="U2I75_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1695431.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            68636..68998
                     /locus_tag="U2I75_000268"
     CDS             68636..68998
                     /locus_tag="U2I75_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1695432.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            69007..69759
                     /locus_tag="U2I75_000269"
     CDS             69007..69759
                     /locus_tag="U2I75_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695433.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            69773..71575
                     /locus_tag="U2I75_000270"
     CDS             69773..71575
                     /locus_tag="U2I75_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695434.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            71852..72892
                     /locus_tag="U2I75_000271"
     CDS             71852..72892
                     /locus_tag="U2I75_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1695435.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(72944..75088)
                     /locus_tag="U2I75_000272"
     CDS             complement(72944..75088)
                     /locus_tag="U2I75_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1695436.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(75445..76818)
                     /locus_tag="U2I75_000273"
     CDS             complement(75445..76818)
                     /locus_tag="U2I75_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1695437.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(76818..77213)
                     /gene="rbsD"
                     /locus_tag="U2I75_000274"
     CDS             complement(76818..77213)
                     /gene="rbsD"
                     /locus_tag="U2I75_000274"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN1695438.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(77241..78158)
                     /gene="rbsK"
                     /locus_tag="U2I75_000275"
     CDS             complement(77241..78158)
                     /gene="rbsK"
                     /locus_tag="U2I75_000275"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN1695439.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(78266..79279)
                     /locus_tag="U2I75_000276"
     CDS             complement(78266..79279)
                     /locus_tag="U2I75_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1695440.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(79360..79959)
                     /locus_tag="U2I75_000277"
     CDS             complement(79360..79959)
                     /locus_tag="U2I75_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1695441.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            80145..80846
                     /locus_tag="U2I75_000278"
     CDS             80145..80846
                     /locus_tag="U2I75_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695442.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            80856..83567
                     /locus_tag="U2I75_000279"
     CDS             80856..83567
                     /locus_tag="U2I75_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695443.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            84018..85646
                     /gene="treC"
                     /locus_tag="U2I75_000280"
     CDS             84018..85646
                     /gene="treC"
                     /locus_tag="U2I75_000280"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN1695444.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(85706..86887)
                     /locus_tag="U2I75_000281"
     CDS             complement(85706..86887)
                     /locus_tag="U2I75_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1695445.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            87139..88662
                     /locus_tag="U2I75_000282"
     CDS             87139..88662
                     /locus_tag="U2I75_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1695446.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKI
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(88731..89459)
                     /locus_tag="U2I75_000283"
     CDS             complement(88731..89459)
                     /locus_tag="U2I75_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1695447.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(89472..90752)
                     /locus_tag="U2I75_000284"
     CDS             complement(89472..90752)
                     /locus_tag="U2I75_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1695448.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(90893..91495)
                     /locus_tag="U2I75_000285"
     CDS             complement(90893..91495)
                     /locus_tag="U2I75_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1695449.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            91740..91946
                     /locus_tag="U2I75_000286"
     CDS             91740..91946
                     /locus_tag="U2I75_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695450.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            92258..92977
                     /locus_tag="U2I75_000287"
     CDS             92258..92977
                     /locus_tag="U2I75_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1695451.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(93058..93741)
                     /locus_tag="U2I75_000288"
     CDS             complement(93058..93741)
                     /locus_tag="U2I75_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN1695452.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            93959..96145
                     /locus_tag="U2I75_000289"
     CDS             93959..96145
                     /locus_tag="U2I75_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1695453.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        31254 a        17073 c        19877 g        28205 t
ORIGIN      
        1 aaataaaaat catactatga tatgtacgcc aatggagggg atgaaatgga aaagccaatt
       61 gaaaaagcgt tagatgaatg tcttttcttt agcgtgaaaa agctagatcg catgttgaat
      121 aaattagctg atgaagcgtt tagaaggaca ggtctggcac caacttatgg ttttatttta
      181 ttgatattaa aagaaaaaga tggaatacca caaaaagata ttgcacaaat gctctattcc
      241 gccccatcaa cgattgctcg ttttgttgaa aaattagaat ataaaggata cgtcaaaaca
      301 gttagcgaag gtcgcttgtc attagtttat cttacagaac aaggacgcca atttgctaag
      361 gaagtcgatg gtagctggga agaacttcat cagtcataca aagcagtttt gggaaatgaa
      421 gcaagtaaag aattagccca gacaatgaat gatgcaacag ataagttaca aaaaaaataa
      481 aattttttaa gcaaattagt tgcatgtaca actaatcgag tggtttaatg atagatgatt
      541 ctcgataggt taatcagagc gaattatggg aggaatgaca atgtcacaat ttgatcaatc
      601 aattacttta ccttcaggag ttcaattgaa gaaccgattg atgctgtcgc ctatgacgac
      661 acaactcagc tttttcaatg gagtgatcac cgaagatgag atcacttatt ataaacaacg
      721 ttctaaaggt cttggtgctg ttattactgg tgctgcaaat gtccaagata tcggaaaagg
      781 ctggccaggt gaactcagca tcgctcatga tgaaatgatt cctcgattaa gtgagctagc
      841 gaaagcaatc caaggagaag gcgcaaaagc gattattcag attttccacg gtggacgaat
      901 gacaagtcga gcaactttat ctggagaaca gcctgtatct gcaagtgcag ttgcagcaaa
      961 acgtcctgat gcggaaacac cacgagcaat gagtgaagaa gaaatcgttg aaacaatcca
     1021 agcatttggc aaagccacac gtagagcgat tgaatcagga tttgatggaa tcgagttgca
     1081 tggagcaaat acgtatttga tccaacaatt tttctctccg cattcgaata gacgagagga
     1141 tcaatggggc ggtagcttgg aaaatcgtta tcgttttatt gaagaattat taacaacggt
     1201 tttctcagcg gttgaacaat atgctaaaaa accattcatc gtagggtatc gtttctcacc
     1261 agaagaatat gaaacaccag gtatccgttt tgaagacacg atctggcttc ttgaacgatt
     1321 acgtgagtca aaactagatt atctgcatgt ttcattaaat atgtatgatc gaatagctcg
     1381 ttctgataaa tacaacgata aatcgattct tgaagtggtc cataacacga ttcaaggaaa
     1441 gatcccttta gttggtgtcg gtgggatccg caatcgcgaa gatgtagagc aggtattgaa
     1501 atatgcagaa cttgcagcaa ttggacagca aatgatcgtc gatcctgact gggcagagaa
     1561 attattggaa aaccgtgagg atgaatttgt aacgaaacct tttgaagaag catataaaga
     1621 gctttatttg ccaagcccat tatataattt cttgaaagca cgttatcaat aacgcaattt
     1681 ttatagaaag aggatgggaa aatgaaagta ttgatcgtat taacaaatgt agaaaagtac
     1741 gaaaagatcg agcgtcctac aggtttgtgg ttaagtgaac tgactcattt ttatgatgta
     1801 ttagtaaaaa atgggatcga tgcagatttt gtcagtccta aaggcggata tgtaccttta
     1861 gaaccgcaaa gtgttttgaa tatggatgaa atcgattggg cctattatga agatgaagat
     1921 ttcagaaatc gggccttggg aaatacctta cgtcctgatg atgtggatgc aaatgattat
     1981 gcagcggttt attatgctgg tggccacgga acaatgtggg attttccaga agcaatagat
     2041 cttgggaaag tcgcttcaca aatctatcga aatgaagggc tggtctcagc tgtttgtcac
     2101 ggtgttgtag gattgttgcc agttacagat gagtctggaa aatctatttt agctggacgt
     2161 acagtgactg ggttcagtaa tgaagaagag gaagcaaatg gtacaacgaa tgaagtacca
     2221 ttcttagcag aagatgcttt gaaagaaaaa ggagcaaact atcagtctgg agcagctttt
     2281 tcagaagtgg ttcaaattga tgggcggctt cttacagggc aaaatcctca atctgctcat
     2341 caattaggcg aagcagttgt tgaagcatta aagaattaaa aataaatgat gtgtctattc
     2401 taactattac attacacacg ctaaaactcg tcttttcgtt cttttctgtt tcctatttgg
     2461 acagaggtac gtaaaaggcg agtttttttc ttttataaga gtacattttt tgaaacatct
     2521 cttcagaaat gatactcttc aattaggtcc actagtacat tattgagaag cagacctaga
     2581 acacggtatg atggagaggg ttgattctgc tgcatcgcta tgtatcataa ggttgaaagc
     2641 gagttatgaa tactgtgttt tctgattgag aagttagcag tagaacacct tataagaaag
     2701 aggaatgcag atgaacattg taattgtcgg cgggtcgttt gcagggattc atgcggctat
     2761 ttatcttcgc caattgatgc caacaagtga aatatatttg attgaaaagc aatcgaagtt
     2821 aggatggatt cccagcgggt tcaatttgat attaaaagga aaggttacca gtgaaaaaca
     2881 actttcttgg atcacgaaag aagaactgac tgaccgctat cgaatccatg tttacacaga
     2941 aaagacagta ataggattga aagaaaaaaa agtcgtttta gcaggaggaa aaaaattgga
     3001 ttttgatcgt ttgattctag cgactggttc aaatcaaaag tttcgaaata tttctgcaga
     3061 atcaacgctt attcacccag ttaaaaagat acaaaacgta acggctcttc agcaaaagat
     3121 catgaatgca aagaaaattg caattattgg tgcaggacaa gcaggaatcg aaatagctga
     3181 agggttggca tcaagaaaaa aacaaatcca tctttacgaa agccgaaagt caattctttt
     3241 tcgttatcta gatcctgaaa tgacagaacc gttagtaaag gaaatgaaaa atcaagggct
     3301 ttcacttttt ttagaagaac aagttgtgtc tttgaccgaa aatgaaaccg ctattgtaga
     3361 gacggaaaaa agaaaggaga actatgatct tgttttgctg gctaatcatt ctcgtcccga
     3421 caatcagatg tgggaagaac aattaacatt gaatgatgat ggaacgatct gggtagatga
     3481 ctacttgcaa acgtctcaaa aagacgttta tgcgattggc gatgcgatcc aagtaacttt
     3541 tcgtccaaca aatgaaaaaa tgtatgtttc tctagtaaat aacgctgtcc gtacagcacg
     3601 aaatgtcagc aagacaatta gtggctcaca gacaaaggat cagggaacct atcgtccagt
     3661 cggcaatcag tggtttggct atttcttagg aagcgtaggt ttgacagaag aagagagcat
     3721 tttttatccc caaaaaattg aaacaggata cgtagactac aagcttacag caataaatca
     3781 aagcaaagtt aaactcaaat ttctatttga ttctgaaaaa aagattcttg gagcacagct
     3841 ccaaagcaaa aaagagatgt tccaccttct tgaccaactg acaattgcag tagaagaagg
     3901 atggacgttt gaacagttag aagaacatga gctatttttc cagccagaat accgtgtgcc
     3961 agatatggtg tggtcaaagg tggcgaatac tgcctatgaa gattgaagca ttattagaaa
     4021 gaaaagagca gttacagatc ctgattttgc gaaaccttgt tctgcaaggg ggaacagcct
     4081 ctatcaatgg tttgagagag catgtccaac tgtcaaaagc ctcgtttgat caatatttgg
     4141 aagatattga attgattggc cgaatgatgg aaaaaaaagt agaagtacag cgaaatgagt
     4201 atcaggcgct gcttgtgttg gacgaagatg taagtttgga aaaaattcta ttgtttttat
     4261 tgcaggaaag cttgaagttt aagatgctcg tctatttatt agaacatcag caagtatcca
     4321 tcgttcgatt agcgacagcg tttaatatca gtgagtcttc tgtttttcga aagatcaaag
     4381 aattgaacca gctcttagag gaatttggat tacaaatcaa aaacggtcaa ttatacggag
     4441 aagagctaca gatacgatat ttttattatg aactattcca atatatcccg gaagatcagc
     4501 gaccgctttt tttgcaaaat acaccagaaa aaagaccgtt cattctagga ttggaccgtg
     4561 tattagaaac aacgtttacg gcatcagcag aagcacagat tgcttgctgg ctcgggatta
     4621 cgaaaaaaag actattgaat gaaaaaagta cgtacgcaac tttgaaagaa aagaaattac
     4681 tctatcagtc ggaccggctt tatcaagcaa tcgatccaat catcgtgatg catcttagtc
     4741 gaacagcagc agaactgaat gtatacgaaa caatgatgtt ttacagcttt tttgtttctt
     4801 tttcgatagt agacgaagaa atattttatc agtatgattt gacacggagc aaaaagttac
     4861 caaccgctgt tttagatact tacattcggg aaacgatgtt atggcattat cgtcctcgtc
     4921 gtttgaaaat caaagaagaa aaggcagtag gctaccaaat ttcccagatc aacaatgaat
     4981 ggtttttctt tgttggaaag atcgaagtct atgagcgtga tcggcttttg gaacagcagc
     5041 agaaaatgct cgggcattcc ttaacacagt tactggcgaa attacaggaa acagcggtgc
     5101 agcaattgcc gaggaaaaga gctgaagata gtgagcttag ttacctgatg atccagtatg
     5161 ccaatgttct tttgatgatc gatttttata ttgcaaaacc agtagtgatt ggaatcgatt
     5221 tagaaagtct gccgatttat cgtattgctt ttcaacaata tctgatcaga gaactgaggg
     5281 gaatcggcgg tattgagatt ggaagttatg aagaaggtaa agaatatgat ttggtgatca
     5341 cattttgcca gagaaacaaa aaacagtgtg aatattatct gtcagaattt gcgtcacctt
     5401 acgacattat tcgattgaaa agaagaatag agacgctgaa aaaagaaaag aattgattaa
     5461 aaaatagctt cataataaaa aactgtcaaa aaaagaaaaa aagaataaaa ttttgacagt
     5521 ttcttttttt aaaacagtag tatgataaga gtagttaaag aaagcgtttg catataatgg
     5581 agggaatcac aatgacagaa tcaacgtata tcatggcgat tgaccaaggt acaactagct
     5641 cacgagcaat tatctttgac aagaaaggaa gacacatcgg cagttctcaa aaagagttca
     5701 cgcaatattt cccgcaagaa agttgggtgg aacatgatgc aaatgaaatc tggaattctg
     5761 tacagtcagt tattgccgga gcgttcatcg aatcagggat caaaccaaat cagattgctg
     5821 ggattggcat aacgaaccag cgtgaaacaa cagtaatatg ggaaaaagat acaggacgtc
     5881 caatctatca tgcagttgtt tggcaatcca gacagtcagc tggaattgca gataacttaa
     5941 aaaatgaagg ctatcaagaa tttttccacg aaaaaacagg gttagtgatc gatgcctatt
     6001 tctcagcaac gaagatacgc tggattcttg atcatgtaga aggcgcgcaa gaacgagctg
     6061 aaaaaggcga attgatgttt ggaacgatcg attcttggtt agtatggaaa ctgaccgatg
     6121 gacaagcaca tgttactgat tactcgaatg ccagtcggac gatgttgttc aatatccatc
     6181 agctagattg ggatcaggag attttagatt tactaaatat tcctcgagtg atgttaccaa
     6241 aagtcacttc taactcagaa gtttatggtt atacgcaagg ttatcatttt tatggaagcg
     6301 aagcaccaat ttcagggatg gcaggagacc agcaagccgc attattcgga caaatggcat
     6361 tcgaaccagg catggtcaag aatacttatg gtacaggttc atttattgtc atgaataccg
     6421 gtgaaaaacc gcaattatcc aaaaataatt tattgacgac aattggttac ggcataaatg
     6481 gaaaggttta ctatgcttta gaaggaagta tttttgtggc tggttcttcg attcaatggc
     6541 tgagagatgg acttcaaatg ttgcaaaaag ctagcgattc agaagacgca gcgaaacgct
     6601 ctacaagcga agatgaggtt tatgttgttc ctgcattcgt tgggctaggt gctccttatt
     6661 gggatcaggc agcacgaggg gctatgtttg gcttgacacg tggtacgaca aaagaagata
     6721 tcattaaagc tactttacag tcgattgctt atcaagtgag agatatcatc gatacgatgc
     6781 aagatgacac tggaatcaag attcctgtat tgaaagtaga tggcggtgca gcaaacaatg
     6841 aatatctgat gcaattccaa acagatatct tgaatgtacc gatccaacga gcagaaaact
     6901 tggaaacaac ggcgctaggg gcagcatttt tagcaggatt agctgtcggt tattggaaag
     6961 atacagacga gatccgcgaa ttttatgaag ctggcaagct atttgaagtt caaatggagg
     7021 aagagcgccg agaaaaactt tacaacggtt ggaaaaaagc agtaaaagct acccaagcat
     7081 ttgaataagg aggaaatcca tatgttttca aacaagacaa gacaagatag cattcaaaaa
     7141 atgcagcaag aagaattgga tctgttgatc atcggtggcg gaatcactgg tgccggtgtg
     7201 gcagtccagt cagcagcatc aggaatcaaa acaggattga tcgaaatgca ggattttgca
     7261 gaagggactt cctctcgctc gaccaaactt gttcatggcg gtattcgtta tctgaagaca
     7321 tttgatgtgg aagtagtagc tgacacagtt ggtgaacgtg cagtcgtaca aagtattgcc
     7381 ccacacatcc caaaaccaga tccaatgctt ttgccaatct atgagggcga aggagcaaca
     7441 accttcaata tgttctcggt caaagtagca atggaccttt atgacaaact ggcaaatgtg
     7501 acaggaacca aatatgagaa ttatacgctg acaccagaag aagtattgga aagagaaccg
     7561 tttttgaaaa aagaagggct aaaaggcgcg ggtgtctatc tagatttccg caacaatgat
     7621 gcccgtttag tgattgataa tatcaaaaag gctgccgaag atggggctta tctagtaagt
     7681 aaaatgaaag cggttggctt tttatatgat ggcgatcaaa tcgttggcgt caaagctcgt
     7741 gacctgctga cagatgaagt gatcgaaatc aaagcaaaat tagtgatcaa tacgagtggt
     7801 ccttgggtag ataaagtacg aaacttgaat tttacgcgtc cagtctctcc taaaatgcgt
     7861 ccaactaaag ggatccattt agttgtagat gcgaaaaaac tgcctgtacc gcaacccaca
     7921 tacttcgata caggaaagca agatgggcgg atggtttttg ctattccaag agaaaacaag
     7981 acttactttg gtacgacaga tacggattac caaggagatt ttacggatcc gaaagtcaca
     8041 caagaagatg tagattatct attggatgtg atcaatcatc gctatccaga agcaaatatc
     8101 acattgaatg atatcgaagc aagctgggca ggacttcgtc cactgttgat tggtaattct
     8161 ggttctgatt acaatggtgg agataatgga tcgatttcag acaagagctt caataaagtt
     8221 gtcgatacag taagtgaata taaggaaaac aaagtttctc gtgctgaagt agaagacgtg
     8281 ttgaaccatt tggaaaacag ccgtgatgaa aaagcacctt ctacgatttc cagaggtagt
     8341 tctttagaaa gagaaccaga tggtttgctg actttatctg gtgggaaaat caccgattac
     8401 cgtaagatgg cagaaggagc tttgcgattg atacgccaat tattaaaaga agaatacgga
     8461 atagagacga aagaaatcga ctctaaaaaa tatcagattt caggtggaaa cttcgatcca
     8521 actaaattag aagaaacagt gacagaatta gcaaaagaag gagtagcagc cggtttagag
     8581 gaagaagatg ctacttatat cgctgatttt tacgggacta atgcccgacg tatctttgaa
     8641 ttagcaaaag aaatggcacc ttatcctggt ttgagtctcg ctgagtcagc tcggttacgt
     8701 tatggtttag aagaagaaat ggttttggcc ccaggcgatt atctcattcg tcgtacaaat
     8761 catctgttgt ttgaacgaga tcagcttgat gagatcaagc aacctgtgat cgatgcaatt
     8821 gctggatatt ttgaatggac agaagaggag aaggcgcaac agactaaacg tttagaagca
     8881 ttgattgcag aatcagatct gcgggaacta aagggggaga aataaatgaa cagtgatatg
     8941 actcaaatca tgggggagtt tattgggaca ctgatcctag ttttgcttgg agatggtgtg
     9001 tgtgcggctg tgaatttaaa caaaagtaaa gcacaggctt ctggctggat tgtcatcgca
     9061 tttggttggg gactcgctgt aacaatggct gtctatattt ctggattcat gggcccagct
     9121 catttgaatc ctgcagtctc tctagctatg gcgatgacag gggcaattag ttggaatctt
     9181 gtcgttcctt tcatcattgc gcaagtttta ggagcttttg caggggccat tcttgtttgg
     9241 ctttcttatt tgccacattg gaatgcaacg aaagatgaaa gcgcgatttt aggaacgttt
     9301 gcaacaggtc ctgctatacg gaattatcca gcaaatgtga tcacagaatt aattggtaca
     9361 tttgtattag ttcttgggtt gctagctttt ggacaaaacg aatttgcacc tggaacgaac
     9421 gtattcgctg taggtggatt gatcttagcc atcggtcttt cattaggagg gccaacagga
     9481 tatgcaatca accctgctcg ggactttggt cctcgattag cacatgcggt attgccaata
     9541 gctaataaag gaacttcaga ttgggcatat tcatgggtac caatcgcagg tccaatgatc
     9601 ggagcaatta tcgcggtagg tgtctattca ttaatggtgt aaatctataa aacatactaa
     9661 agtttgacaa ccaaaatgtg tcttgctata gttaagtgga aaataaataa gacctagaag
     9721 aatggggtat gttttgtgag tgtagctggt atctaatcag taagtcgaat agccaataaa
     9781 caaagtttat ttggtgtgct tatgaaacgg tacctgtcag gaaaacaata tctttgtatt
     9841 ctaataagga taggacatga cttttttaag ctgtgttcta tccttttatt tatctctagg
     9901 cccttattta ttttgggaca ggtatctctt caagaaaagg agagaatatg gacaacgaaa
     9961 tgattagtca attacaattt gatcggatca aaaaggaaat ccaagcaaga gcaattggaa
    10021 actatagcaa aaaacgaatc agcgatatga cggtctcgac aaacctacaa actgtcttgg
    10081 atcgtcaaga agaaacaaga gaagcacgct tgatcttaga aagcagtcag catgttccat
    10141 ttatggggtt gccgaggatc gatgcactga cagaacaagt caaaaaaggg ttgattctgc
    10201 agccaaccga tttgatcgaa tatgcggatt ttcttagaag cagtcggatg atcacaaagt
    10261 tttttgataa gaatcaatac cagacaccct tgttgtttgc ttatagcaaa catcttcctg
    10321 atttgataaa tgtagaagag ttgattgacc agaaaataaa aaataataaa gtcagtgacg
    10381 acgcgtcacg taatttgcgt aaagtacgca aacaattaca actaatcgaa aaagaaatcc
    10441 agtcaaaatt actgaaattc ttgcgtcatc cgaaaaacaa agaaatgatc caagaagcaa
    10501 tgattgttca aaaaggcgaa tactatacga ttcctatcaa agccagctat aaaaataaaa
    10561 tagatggaac gattatcgat gaatcaaata aaagaacgac ggtttttatt gaaccaactg
    10621 tgatttctaa attgaatgaa cactatcagt tgttaaaagc tgaagaaatc agtgaagaat
    10681 atcaagtcct agcggcattg actggtgcta tcgcagaaaa cgaagaagtg atcgatttat
    10741 tgatcgaaac gatcacggtg ttagatatca tctttgctcg ggcaaaattt agccgagaaa
    10801 tcaatggaag cacacctaga atcaataaat cggaacatat cattatcaag caaggcagac
    10861 atccattttt acctgagcat gcggtaccat tggatgttga aatcggtaaa gactatcgag
    10921 gcttgattat caccggagca aatgccggag gcaaaacagt cgtgttgaag acagtcggtt
    10981 tattgacttt gatggcgatg ttcggtatgc aagttccggc aaaagaaggg acagagcttg
    11041 ctgtctttga tgagattttc gtcgatgtcg gtgaccacca aaatttagaa aatgcgttga
    11101 gtacattttc tggtcatatg caaaacatcg ctgctatttt gaaaaagata aaaaggaata
    11161 cgttggtact tttagatgag attggcagtg gaacagagcc aaatgaaggg gctgcactag
    11221 cgatcgcaat catggaatct atgtatgagc aaggggcatt gatcattgcc acgacccatt
    11281 atggagaaat caagaaattc gccggagatc atgaagattt tgtgccggct gctatggctt
    11341 tcgatcggga agcacttcgt cctaaatatc agttgcgggt tggaagaaac aggagaaagc
    11401 caagctttgt ggattgctca taaaatggat atgtcgatga aattgatcca tcaagcagag
    11461 cgttacttgg cggaaaaagc ctatcggaca cagaaaaaag aatttcttcc taagacagca
    11521 gttacaaata ggaaggagaa taaaaaagaa cgtcagctct ttgcaaaagg tgatcggata
    11581 tttgtcaatg aataccaaaa agaagcgtta gtttatgaag atataggtga agatacgatt
    11641 gatgtatatt tagacaaaaa aataatccac gtgcctcgtc agcgtgttcg tctggtgcgt
    11701 tcagcagaag atctgtaccc gacaggttac gatttagaca gtctgtttat cgattataaa
    11761 acacgtaaac ggcagcgtga cctagagcga ggatcaaaaa aagctcataa agtactagta
    11821 aaagaaatgc gcaaacgaca ggaagaaaga agagtaaacg atgaaaacag caagtgatta
    11881 tctcaaatta gctcataata tcagtgtctg taaagaaaaa taaaaacagt gaaatcattg
    11941 gttttattcc ccgtgtaagc taaaataaaa agaaaagagt gctgaggagg tagggcgata
    12001 aaatgaaatt aagcacagac tggcgtaaag aaatacaaac gattccgaat cttttatcga
    12061 tattccgtat ttttttgctg ccgatttatc tttattttgt gctgagacag tcattttata
    12121 tagcaggagc ggtcattgtt gtctcaggtt tgtctgatta tctagacggg gtcattgctc
    12181 ggagatataa tcaggtaaca gacttaggga aggtgcttga tccgtttgca gataaattga
    12241 cacaactttt tctgattttg tctatggcat ggtatcgacc gtggctttgg ctgttgtttg
    12301 gtctattctt gataaaagaa ggctttatgt ttgtagctgg attaatcggg ctatctaaaa
    12361 atatcaaatt aagtggagcg aagtggtatg gcaaagtagc gactgctgtc atctatgttg
    12421 gcatgatcct tttgctgctt ttcccagaat taccaacgct gtgggtgaga gtcatttttg
    12481 ccgtcatcac ttacggtctt ttacaatctt ttgtcttgta tgctgtcgag taccgaaaga
    12541 tgtttcaaag aaaataaaaa aggggtgtga cctatgtcgc attccttttt ttgataaata
    12601 gactcccagt agctacttct cgataataag ccaaccgaac caaaaacagg agaagttgtt
    12661 tttgaaatta tttcttgtga ttcggagtta aatggtaggc tcacaacctc ttggtaaacg
    12721 gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg agcagctatt
    12781 tttccgtttg tttctctaat actcaggtca aaccgctgat tttacaacct cttccttaaa
    12841 cggtattcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaatcc
    12901 attttcacaa aatttttctt aatactcgaa actaagcgac tttttcacaa tctcttcctt
    12961 aaacggtgtt caatcagctg accttcggta ttagctcaaa aaacggcaaa atatgaggag
    13021 ctattttccc gtttttttct ctaatactta ggtcaaaccg ctgatttcac aacctcttcc
    13081 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatatga
    13141 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagcgttt tcacaacctt
    13201 tgattcacgg ttttccagaa gtagcttctt cgaaaaataa gccgaaattt cttgaagctg
    13261 atcacttctg tcgcaacctc tactgactaa ggcttgatat aagcataacc ttgggattgc
    13321 agttcaacta aatcaaggac acctgctggt acaacgatta cttgttcagg taaagaactt
    13381 tctgaaatat gattagcacg catagcattg gcgcaagcgt gaaaaacgac tccttgcaaa
    13441 tctaagaatt ctgcattggc agagtctaga taaccggtaa tcgcgatgcc atttacagat
    13501 acgacaatcg tcgtttctgg agaagctttt atcaggtttt ttacgttttt ccctgtttct
    13561 gaccattttt ctaattcatc aatatggaaa acgactttca ttgaaatcat tcctttactt
    13621 tttaataatc atcttgatcg cttgttctag tttagccgct tgttcttctg gattactatc
    13681 aggtttctca aagcaatttt tcagattatc tgcaacgatc atgcccatta cgcgatcaat
    13741 actagaacga acagcgctta attgagtgat gacatccata cattcttttt cgtcttcgat
    13801 cattttttgg ataccacgaa tttgtccctc tgtacgtttc aaacggttca ggatattttt
    13861 attatcaatc tccataacac attctcctct atttttataa ttagataata ccccttatcg
    13921 gtattttatg caaatataaa aagtagtgaa aaaaatgaag agtcattctg gttgacatat
    13981 taataaagct cctgtttaat ataccccatg aggtatttat aaaaatgtaa atgaggtgca
    14041 gaaaatgaat ttgctatttc aatcaataaa atcgataaca gtcccagaat taaaggaaaa
    14101 gcttttggaa aatccagcgt tacttgatgt tagaacacct gcagaatacc ggggcgggca
    14161 tataaaagga gctaaaaatg ttccattaca aagtataaac agatatgatg gagacaaaga
    14221 aaagaccgtc tatgtgattt gtcaatcagg gatgaggagt aaacaagcag ctaaagaatt
    14281 aaagaaatct ggttatgatg tagtgaatgt acgtggtggc atgaatcaat ggtttgatcg
    14341 aacagtagga gggaaataag atgaaaatcg ttattgtcgg aggtgtagca ggtggaatgt
    14401 ctgctgctac acggcttcgc cgattaatgg aagatgcaga aatcgttgtt tttgaaaaag
    14461 gaccgtatgt ttcttttgca aattgcggct tgccttatta tctttcggga gaaatcagtg
    14521 aacgggaaaa tcttcttgtc caaacgccag aatcattatc cgctcgtttt tgtttagatg
    14581 tgcgtccaaa tcatgaagtg acagccatct ttcccgaaaa caaaacggta gaagtcgtac
    14641 atgagggtca aaaacacatt gaacagtacg atgcattggt tttatctcct ggtgcaaaac
    14701 cagttgttcc atcgattcca gggataacag aagccgacaa tgttttttct attagaaatg
    14761 taccagatat cgataaagtg atgcatgcat tagaaaaaca gccaaagcgt gccgtgatcg
    14821 ttggtgcagg attcatcgga ttggaaatgg cagaaaacct aaaaagaaga ggtttagaag
    14881 tcatggtgat cgaacaagca ccacatattc ttccaacact ggatgaagaa atggcagctt
    14941 ttatagaaaa agaattgtct catcaaggag tagaagtgat tacttctcat gctgtcgctg
    15001 gatttgaaga ccacgggaaa cgattgcgac tggatgatgg gcgtaccatc cctgctgatt
    15061 tagttatttt atccattggt gttcgtcctg ataaccagct agcagtgacc gctggaatcg
    15121 aattaggcat acgcgggggt atcctagtag acgaacgtta tcaaacgaat attcctgata
    15181 tttatgcggt gggggatgct atcgttgtaa aacagcaaat cactggaaaa gatgcactta
    15241 tttctcttgc ttcaccagcc aatcgtcaag gtagacaagt tgcggacacg atttccggaa
    15301 tttctcgaag aaatcaaggc ggtattggaa cagcaattat acgaacgttt ggaatgactg
    15361 ccgcatccac cggtttaagt gaaagaacag ccaaagaaaa cgaactgtct tttgaagtca
    15421 ttcatgtatc aggaaaagat catgcaagct attatccaga agcaacagat attttactga
    15481 agttgatctt ccatccagag actggcgaga tttatggtgc acaaggtgtt ggggcaaaag
    15541 gtgtggataa acggatcgat attttagcaa cagcaatcaa agggcatttg acgatcttcg
    15601 atttaccgga attagaattg acgtatgcac cgccatttgg ctcagccaaa gatccagtaa
    15661 acatgctagg atatgcagca atgaacattg cagaagggct tagtgaaacc gtacaatggc
    15721 atgaattgcc gacagaacta gcaaaaggaa aaattttatt agatgtgcga acagcagaag
    15781 aattggaaaa gggcaaattc aaggaagcca aacatatccc tttgaatgaa cttcgggacc
    15841 gattaaatga attagacagc caacaagaat atatcgtcag ctgtcatagc ggattacgta
    15901 gctatctagc ggaaagaatc ttgaagcagt ctggctacca cgtaaaaaac cttgatggtg
    15961 cattttcttt atatcaaact gtccgacctg aagaactgat atatcctaac aaatgatact
    16021 cgttaaaaag cttgcattcg actcttaaaa gaggtaaact aatcggttcc accactttaa
    16081 aaaaagagga gaatgtgtat ggtcaacaca caaaaacagt tagaagaagc atatctgaag
    16141 aacgtgtatc aaaagctaat tgataaaaaa aacgaactgc gggaatcgat ggatacggca
    16201 aaaatagaag gaaaccagtc gttgaaagaa atggctggcg agcttcgatt gaattttgac
    16261 agttacttag ataacctgga tactttttcg atgattgaaa tgaaaaatag agaaatcgat
    16321 cagatgaata tcaaacagaa aaacgcagaa aatcagctag caaagatcga acgtttattg
    16381 aaaagtccct actttggaaa agtcgttgta gactttctgg ataatgagcc aaaagaatcc
    16441 ttttatatcg gtacagcaaa tttcacagat gatgcagatg aaaatcttgt ttatgattgg
    16501 cgttcaccaa ttgccgaact tttttacaat aacatgattg gtccatcttc ttatacggtt
    16561 cgtcaaaata aaatcgatac ggttattaaa gaaagacgac aatttctttt ggaaagagat
    16621 cgtttggtca agttttttga tacgtctgta gctattcaag atgacgtatt attagaagtc
    16681 ttaggccaag atgagaccaa tgaaatgaag gctatcactg ctacgatcca atcggaacaa
    16741 aacacaatca ttcgggatgt gaaaagtcaa aatatcttgg taaatggcgt agcaggaagt
    16801 ggtaaaacct ctgctatcat gcagcgtatt gcgtatctgc tctatttata tcgagatacg
    16861 atgacatccg atgacttgct gattctatca cctaatcatc ggtttatcga ctatatttcg
    16921 aatgtcctac catcattagg tgaaagaaat ccactgaatc taaccatcat acagctggtt
    16981 tcacagcttt cagcagagga aatagaaggt gaagaggctt atttcaaacg tatcacggga
    17041 gaaaatgtat cggagcagac agaacgtttg cgtagcaaaa agtttatcga taatctaaaa
    17101 caatcagatc ctttgttttt agatcatccg aattttatcc gtggacttac taaaaatgga
    17161 aaaacagtcc tttctaaaaa aacgatcgaa aaaatatatg agaaagttcc tgctcatcct
    17221 aaattgatcg ataggcttca agcaacgaaa aaggcactga tgagcgaatg gaagaatcat
    17281 cttttgaaac aagcaaaaag tcctgctgtg cagaatcagg tactctcgtt gacagaagat
    17341 aggcagttgg aattattcgg taaattgatt tctgatgatt cggaacaaag tattgtagct
    17401 tatgcaagga aacttttaca aaaaaaatat cgaaaaataa ccagacaaat cgaagaaatg
    17461 gcatgggtgg atgagcatca gttgtttgaa agaatttatg aaaaacgata tggctcagct
    17521 tatgcttggc aacccacacg tactgttgat gaagcagtca ttatattagc tattcgtcat
    17581 ttacttgttg aaaaggtaaa tgtaccagca tttcgctatc tattgattga tgaagtacaa
    17641 gattataccc tggcacaatt aggtctattg atcgaattat ttccaaaaac tcactttact
    17701 ttagtaggtg acgagaatca ggcaatcttt aattcttcca ctacttttgc tgatattatg
    17761 cgttgtttcg acgactacca tctgccgatc catcggtatg atcttagaaa tagttaccgt
    17821 tctagcggag caatcacaga gcttttcaaa acctatgctg tggatcaaga gaagatcgac
    17881 atcgtatcga tccgaccgca aggaaaagaa cccgaatacc gctcttttcg atcctctgaa
    17941 gaattgttga aaatactagc tgagattttg gtttctctaa aaggagaaaa agcggcagtt
    18001 atcacccaaa cagaagagga aacccaaacg ctacaagaat cgattaaaaa aaattcatat
    18061 ggattagaaa actgccaaat cattcctttg agtctagcaa aaggactgga atttgatcat
    18121 gtaatcttat atccatttga aaacgatgga gatgagcaaa gaagacgcag acaaatgtac
    18181 acggcgatat ctagaggaat gaaaagcatt gtcgtattgg aaagagcaac ataattaaga
    18241 gataaaaaat aaaaagaaca actgataaaa agaacaaaat atttttcatt tgctgatgag
    18301 aaatgttttg ttctttttct taggaaaaat ttaggaacac tttatgtgaa aagaaatgaa
    18361 atgtatggta aactcctacg tcgtaacaaa aaaacgtgag gtgaggatat gaaaaaagta
    18421 acaagagaag aagttgcatc aattttggtg aaagataagt atttttcaaa aggtaattac
    18481 tggttaaaga tttggcaaac gttagtggct cttttaggat ggatgatcgt tgttcttcct
    18541 tttatatggg tctttttccc attaacgaga ccagaaagag ccaaggattt ttccttgtac
    18601 gcttttttat cagaaaagaa aatgctgtat ttcttaatcg gcttttttgt attgattttt
    18661 ttcagtttcc tgattacctc atttgtattg actaggtgga acaatcgaaa tctatacaaa
    18721 aaagtaaata aatcttttga atatgaagat gaaaaattga aaaaaagaca agaattactt
    18781 gaagaagtgt atacggaacg ttttggaaca aaagaagaga gagaaacggt ccgtttttat
    18841 tctgtctccg aagagaaaaa tctagatacg acattcattg cagatttgta taaagaaaat
    18901 ggggtggaac tgaaatgatc tcaatcaccc atacaggtaa tttattctta gatacttgtt
    18961 tgtctattat gtattttttt ctcgtttctt atccgatttt aggcggattt gtatggttta
    19021 ttggtgtctg gtgttatgtg tttttataca aacataagca aaaagaatgg gtagatgttc
    19081 ccttgagtgt cgaaccgttc atcacgatca tggtgccagc ccataatgaa gaaattgtga
    19141 tcgaatatac aatcgagtat ttgatgacga aattgaatta tcacaactat gaggtattag
    19201 tgacagacga tggttcaacg gaccaaacac cagaaatatt agctagactg atgaaaaaat
    19261 acgctaatct tcgtgtggtt cggatcgaaa aaaataaagg aaaagcccac gcgtttaata
    19321 tcggacttgc ctttgcaaaa gggaaattga ttttaagcaa tgatgcagac acggtaccgg
    19381 agccagatgc actgattagg tatgtgaatt atttcattcg ccctggtgca agacatattg
    19441 ctgcggtcac tgcaaatatg gatgtccaaa atcgtaccaa attgattgca aaatctcaaa
    19501 cggttgaatt ctcaagtatc gtgggcatca ttaaacgaac acagtcagct gttttcggtg
    19561 gtctttatgc gtacagtgga gcaaatacga tgtatcgaaa agaagccttg atcgatgtgg
    19621 gcggattcag acaagatcga gcgacagaag acatcagtat tgcttgggat catcagttaa
    19681 atgactggtt atctgttttt gctcctgaga ttatcttttt catggaagta cctgtgacgt
    19741 tgaagatgct atatcgacac agaaaacgtt gggcaaaagg tggaacagaa gtctggctga
    19801 cgaatttcaa aaaagtgatg ctccatcctt ttaaacatat tggaagaacg atcatcttca
    19861 ttgatcaaac attgagtatt gtctggtcaa tattcttttg tatttccgta gttctttttg
    19921 ctggattaat cgggcattat gtatatcaag gaaactacga acagatttat attactttta
    19981 cattcagttt tgtgttcatc tgctttgaaa tggtcgcagg atttttccag ttgctagctt
    20041 cgctgattgt agatgatcga cgaagaaaat tgaaatacct tcttttcgca ccactatata
    20101 tgcttttgtt ctggatagtg aacgcgataa cgatcgtgac gactttcatt ccagcagtca
    20161 aaacgatttt aggttatgga agcgggacat ggaaaagccc ggaacgtaca aaaaaataaa
    20221 agatgcaccg ttttttagac aaacagcctg ttgtgtttat ggttcttcca aacatttttg
    20281 cttataataa gaaattagtt aaaaagtagt aaggatggga aaagtgatga aaaaaagaat
    20341 gaagccgtta atctggccaa ttgctgtttc cagtattttc ttattgggac agacagcttt
    20401 tgcggaaact tctgaacctg ctcaaacaga accttcaaca accataagct cttctggtgc
    20461 tgaaaacggt gcctcggaaa cagaaatgac ttccaatgta ccgacagata attcaggaga
    20521 aacgatttca tcttcagaag atacatcaat aaatagttct acctcttctg accaaatcga
    20581 tcatttgcaa gacttggaaa ataaaagaaa agaactcgaa gaagcattag aagctgcaaa
    20641 aaacaaattg aaagaagacc aagcagcaat cgatcaagat ttagctgatc ttaaaaataa
    20701 agtgggaaat gaccgtgcac aacttgagaa aaagcttgcc gaagcgaaag caaaagcgga
    20761 agctgtccaa acagatcttg acgggaagat ccaacaagca aaagaattca atcagcaatt
    20821 aaatcaggtt gctcaaacag cttctggaaa gctaagtcaa actcaagcgg atatcacagc
    20881 ggccagtcaa gcaatccacg gaaagatcag cgaagcacaa acagttgcta atgattttga
    20941 agcaaaaaaa gctgacttag cagctagttt ccaacgaata aaagatattt tgcattcggc
    21001 aggagaaaaa tcagtgacct ctggaagcac gacagaaaca gttcagcaaa cgacagagcc
    21061 atccaaatca acaagtcaga ctttgccaaa aacaaatgaa aaaacagctt ctgtcagtta
    21121 ttccttagca ggggcgggtg tgttagtcgc agcagcttac ggaatcatcc gcaaaaagaa
    21181 ataaaaaaga gaggatggga aggtagagta cctcgaagtt agactgaaaa atctaattct
    21241 tgagtgccac tacatgtccc atctctcttt agttattaaa acagatgtcc cctacttatc
    21301 tcgtggagac agctgttcca ctacatgtga ccatcagcat gccattgtcg ctgcctaata
    21361 cttcatagtc catttttacg ccaatgatcg catcagctcc taatgctttt gcacgttcgg
    21421 ccatttcggc aagtgcttct tctcttgcac ccattagttc gttttcatag ctttttgagc
    21481 ggccgccaaa gatatttcgc aaccccgcct ccaagtcttt aaatgcatgg atcccagtaa
    21541 tgacctcacc aaaaacgatc cctttataag ctactacggc ttttccctct acactgttcg
    21601 ttgttgttgt aatcattcga tcactcctct cttttaatta aagtataaca aacttcaaga
    21661 agcactacac atattttccc aaaattagaa atttattata tacttgaatt gttcattaat
    21721 gaaagcgaaa ccaatcgtta gcaactatgt aaaagaaagg aagattcttg tgaaaaaaag
    21781 tattttgctt atttctcata gtcaaaaagt aacagatggt attaaagaaa tgattgagca
    21841 gatgcaaaaa tccgaagagg tgcaaatttt ttctctcggc ggaataaatg gcgagatcgg
    21901 atcagacccg atgaaaatcg tagaagcagt caatgaatca agcgatgcag ctagttattt
    21961 tgtttttgcg gatatcggta gcgcggtatt gaactcggaa ctggcaaaag acatgttgga
    22021 agaagagcag cagcaacgtt atcatctggt ggatgcgcct ttagtggaag gagcttttgc
    22081 agcagccatc actgccggta taacagatga tactcagcaa atcttaacag aagcacaaaa
    22141 tgccggaaag aaaggatggg aataaattga aaaaaatcat gaatgatcca agcaatattg
    22201 tcgaagaaat gctggaagga ttagtaaaaa gctatccaga acttgttcat cgtgtagaat
    22261 cttcccgtgt tgtagccaag aatcagaaag cggaacaagt tggtttggtt tctggtggag
    22321 gaagcggaca tgaaccatcc catgccgggt ttgtagggga agggatgtta agtgcagcgg
    22381 tgcttggtga tgtgtttact tctccaactc cagatcagat tcagacggct attaaggaag
    22441 ccgatagtgg aaaaggagtt ttgctgatcg ttaagaatta tacaggggat gcattgaact
    22501 tcgatatggc aaaggaatta gctgctatgg acgatattga agtagaaagt gtgatcgtag
    22561 atgatgatat cgccgttgaa aatagtacgt atactgctgg aaaacgcgga gttgcaggta
    22621 ctgtccttgt acacaaaatc gtgggcgatg cagcaagaaa cggtgcttcc ctagctgagt
    22681 tgaaagaact gggagaaaag gtcgtacagg caactaagac gatcggtcta gcgcttcgag
    22741 cagcaactgt accagaagta gggaaaccag gattcgaact aggagaagat gagatcgagt
    22801 atggtgttgg tatccacggt gagccaggct atcgtcgaga aaagatgcag ccttctaaag
    22861 tactggcaaa ggaacttgtt actaagattt tagatgatta ctcgaattta ccaaaagaag
    22921 caggtgtact agtcaacgga atgggcggga cgcctttgat ggaacagttc gtgttcatga
    22981 atgatgtttt agctctgttg gaagaaagag gcgtcaatgt agttttccga aaagtcggta
    23041 attttatgac ctcactagat atgcaaggtc tttctctaac actgatcgac ttgacggaga
    23101 cacaatggaa agattcttta gaaagcaatg tacagacgat ttcatggtaa aaaatctcaa
    23161 gataaaaggg tgataatatg aaactaaccg ttcaagaaat ccaagaatgg ctcacgcaat
    23221 ttgcagaggc aatcaatcag aacaaacaat atctcagtga tctagatacc ccgattggcg
    23281 atggagacca tggaaataac atgggaagag gggtttcagc ttatgaagaa gcttttcaag
    23341 ccgatcatcc tgaaacaatc agcgatacat tcaaagtatt ctctatggcg atgatatcga
    23401 aagtcggtgg ggcttctggt ccgttatacg gatctgcatt catgaacatg atgaaagcga
    23461 caaaagaggt cgattctatt gattctcaag aaaaattagg cgaagtgatc gagcagggaa
    23521 cagcgggtat tcaaagccga ggaaaggcag aagctgacga taaaacgatg ttagatgttt
    23581 ggctgccagt cacagaggca ttgaaggcag ggaatttaac aaaagaaatc attgaccagg
    23641 caaaagaaca caccaaagtc ttggtagcta aaaaaggacg agcttcctat ctaggagagc
    23701 gtgcaatcgg acatatcgac ccaggagctg cttctagtgc cattttattt tcaacgcttt
    23761 tagatgtgat caatcaacct taagttcaat aaaactgcgg tgatgctcat cgcagttttt
    23821 tgtttggctg ttcttatgac ttgatgcaga acagcttttt cacaacctct atttgtccat
    23881 atcgcaaaat tcaggtagaa tacaaaagat aacggaaaga ggcgaaacaa tggaacttgg
    23941 aaaatcagat tatcagttgt ttgatcgacc aatctacgca ttcaaacagc tcaaagagag
    24001 ccatccaaca gataagattg aacaaatcaa gaaagaatat aaagaacact ggcaaaaatg
    24061 gaaagagatt cagctccaga cagctgcact tttgcctgat atgtacggca tgagcaaacc
    24121 taaaatcgaa agctggacga atggctggaa tttacgcagt catttttgga gcgcgtaccg
    24181 aagcgaatct cgtcaagacg agaatgcctg tctagcagtt ttgttgaatc aaaaacaata
    24241 ccaaatctat ttgatgtacc aacattataa aagcgaagaa cgatatggta gcattgcagc
    24301 gtataaccag ttgcttgcga tactgaaaga gtggagcaaa acagtcgata tcaaggatta
    24361 ttacatatgg ccgcaaccag aacatgaact tgacgaccac ttagctttgt ctgactattt
    24421 gtccgataca aagaaacaag aagagttgaa aaaagcgatg agagaccgaa cctttcaaat
    24481 gggtaaacta ttcttctatc cgcaagaaat cgaacacgtt gagcaaataa ccgctgaaac
    24541 cttacaagaa ctagcaccac tatatcaaaa gctaggtgcg gaaaaatttc aatagacaac
    24601 acgaaataag caatgcgaaa aacaaacctt actctcggct tgcgagtgaa ggtttgtttt
    24661 tgattattca aaaagtaaat atttaaaaaa ttgataggaa tcaattactt aaggtctcaa
    24721 aagaaggaaa ataaagcaag ggacaaaaag aaatccactc tatttgctgg tatctggttc
    24781 gcttgacgtt tatgctatga taaaagaaaa ttatcagttg gaataaggga agttatgttt
    24841 ctttattaga aatagctgtt tagaaggagg agagattatg agtcatgatc atttatgtgt
    24901 ttcatttgtt cctttgttca atcacttagt acagcaagat caagaaaaag tacacgcact
    24961 tgtcaaacat cactatgtgg aaaaaggaga acagattctt gcgccgaatg gagaacctca
    25021 attagtgatc gtggcctccg gcagtatgaa agtgtatcaa ttatctgcta gcggaaagga
    25081 acagctttta cgagtaatag agccgggcgg atatgaggga gagagccagt tgctaggcgt
    25141 tccaaatgag tctttatttg gcgaagcact agaaaaaacg gaaatttgca tattgagaca
    25201 aaaagatttc tcggaactct tactgcatta tcccgccctt agtctgaaac ttttagcaat
    25261 caacgttgag aaattaacaa aagtacagca gcagacaact tttttgatga tggaaaaagt
    25321 agaagaacga gtcgctgtct atctgcttga cttagctaaa gcgacaggga aggatcactt
    25381 tttcctgcca atgaaaatga aagaacttgc tgcatttatc ggtacgaccc ccgaaacttt
    25441 atctagaaaa tttaaattat tagaggagaa tggttatgta aaaagaaaga atcgagaagt
    25501 ctggatcatt gataaagagc agttagaaga cttgtaaata aaaaaatgac ctccaaatgt
    25561 tggatttttg acccagattt ggaggctagc atgaaattgt acatgctttt ttattatcct
    25621 tctagtaagt ctcttcgcct ataaccaata gaaccaagga cgaacagtgc aaggctgata
    25681 catgataacg taaggaacac ggaaagatca aaagaatcgt ttggcatccg tggaacccag
    25741 ctagggacag ctgttttgga aaaccattct ggtaaatcca aaattccgcc aaagtaattg
    25801 agtgcaaagg agtaagctaa gtagatataa gcaagtttac cgattcgagg aagccatccg
    25861 aatagaaagc cactaattcc gataaacact aaaacggcag gcaggaaatt gaacccggct
    25921 ttaagaaaca tcaatagatc catgtctttc gcatccttca tcgaagccaa agcagaaagc
    25981 cctagtgcac cagaactaat caggattgcc aatatagcaa atagaatagc tagtaacata
    26041 ttggtccaat aaagttggtt cctagaaatt tttgttgcga acagctgatt catacgtccg
    26101 tgggtttctt ctgtaaataa acgattgatg ctagcaattg gtaaaatagc agctagacaa
    26161 atcaagacta ccatgatcgt gcttgtaaag tttttttcaa ttgagacgcc agattggata
    26221 aacatctgtt tcattaagtc gttactttct aaaaactgtt gcatattccc gtagatagag
    26281 ccgtatgccg cccccatcaa accaaaagtg ataagccaac tgattgtcat ccctttattt
    26341 agatagaata ataaaccacg caaggataaa agagaacgtt tagcatacgg ttttccttca
    26401 cgttcgggga tatagccggt gttcaaatct cgatgttcct ctaaaacaaa tgaactgatt
    26461 atcaagatta gactgaacaa aaacaaataa agcaaaggaa tccagttgtt agttgtaaat
    26521 ggataggtca aataagacca gcctaaagga ttccacatcg aaagggagga atcagctaca
    26581 tctgttattg ctcgtagaag gtaaagtcca cctaaaaacg ctaaagacag accattcgaa
    26641 ctggctgaac ttggcatcag ttgtgcagca aacagtgcaa cggcactccc caaaatcccc
    26701 atactaccta cagatagccc aaaaagccaa gcaccttttg cagtgatcga atccgcttgg
    26761 aaaaaaatca tcgtacagct gatcgatatt gctaaaagca aattgatcag aatactttca
    26821 gcgatgacag ctaaagagtt tgcttgacgt ccgactcgaa aggaacgaat aagttcaatc
    26881 agaccaagtt cttctttttt tctcgtttgg ctcaccacgt gtaaaacaga gatcacagca
    26941 gcaaacaaac tgcaaaaaag caacatttca tttgcataca tcgctcctaa agtatactca
    27001 gcagcattcg aagctggagt tggtccgacc attgcaatca ttgccgggtt ttgcaaggtt
    27061 tcgtataatc cagctaatcc ttgtccggaa gtcaattctt caaacgcagg gacaaaacca
    27121 ccagaaaaag cagtgacacc gactagccat atcaggatct ttttccagtc acggctaagg
    27181 ttttgagaaa aaagggtgga ccagcgtgaa aatttatcag tcatttcgct tcctcctcat
    27241 ccttcataat gtctcatgaa taaatcctca agagtcggtg gaatagattc aaattttttc
    27301 acacctaatt tggacgcttc agctaaaatc acatcgaaag cttcgttatc agcagcaaat
    27361 tgagcgaggt gacctttttg cacaaagtca tggacaccgt tcaattgtgc taagtgacta
    27421 ttctctgctt ctgtttctat agtgatggtc gaacgagtca aatgacgcaa ttcatcgagt
    27481 gttccttctt catcgagtgt tccttcgcgt atgatcacga ctttatccgc tagtcgctcg
    27541 acttcactga gaatatgaga agaaagtagg atggatttgc cctgttcttt caatttttca
    27601 acttcttctt ggaaaatcgt ttccatcaaa ggatctaaac cagaagtagg ctcatcaaat
    27661 aaatagagat ccgaatcaac agccaaagca gcaatcaagc ccactttttg gcgatttcct
    27721 tttgaatagt tttttgcttt tttcttagga tcaagagaga aacgttgaat cagttcatct
    27781 tttttttgag cactcccttt tccatgaagc ttcatgaaga gatcgatgat cgctcctcca
    27841 gtcaaatttc cccacaaaga gatatcgcct ggaacataag aaagacgttc atggatagat
    27901 aaactatctt cccatacatc ttgcccaaaa atcgttgccg ttccgccgtc tttttttata
    27961 atccctaaaa gtgtccgaat cgtcgtggat tttccagaac catttggtcc gataaatcca
    28021 acgacttcac ctgaatgtac ggtgaaagag acatctttta aagcttcgaa acgaccaaag
    28081 ctctttttca aatgtgtgac tttgacgatt tctgccataa aaaccccact cctttttcta
    28141 aaaatatata ctaaacaaga ttaaatagtt taataaaaag gttttatacc tttttataaa
    28201 actttttttt attattttag tacataatat tctttttata aatttctgtc aatgaattca
    28261 taaacaagga attgctagct tgatactttc tcaatagata gttataataa acctcagaat
    28321 aaatttgaac tatatatatt ataaaagttt aaaattacgg gaggctctac aaatgaacgg
    28381 ttttgaaaag agagcacaag agaaaaagaa agagatttta caagcagctt ttgacttgat
    28441 gaacacagac ataggaaccg ctggagtgac aatggataag gtagctgaga acgctcaggt
    28501 aagtaaagcg acgattttta aatattttgg cagcaaggaa gggctgattt atgaagtatt
    28561 ttttgcattt atgaatgatt tagggcaaga agcgaaagcg ttgattgcgg aaaatcttcc
    28621 atttgaagaa acactgattg ccatgagtga aaacaaaatc cgttatctca atcgagtgaa
    28681 caaacaattt tatatcgatt tgatgacata ctatacaaaa aaagaaaaaa aagaattttc
    28741 tgacgcgatg gatgcgtata cgcaagaaag tttcagtatc atgcttgatc ttttccatcg
    28801 agggcggaaa gaaggaaaag tggatttgaa atattcggat gagttcttga tcctctattt
    28861 tcaagcaatg gtagaaggaa tttctaagcc ggagatatac gcaaaactgc ttccatatac
    28921 agcagaatgg acagaagtcc tgatcaaggg aatcgcaccg aagaagaaat aaagcataaa
    28981 aaagccgaaa ctgacgataa aatgtcagtt cggctttttt ggttcataaa tgacgtaaaa
    29041 tcaagcagat tcgcgtgctt ctttgtcata gacatcatgg acattgatga atggatcaag
    29101 aataggattt tttccgtcta aatcagattg aaaacgaatg ccatcgatat tgatccgagc
    29161 atttcgatag aaatccgtac ctaattgtcc ccaagctggt gtagaagcat cgacattaca
    29221 gaaaatatcg aaaccttgac tcttcaagta agcaaatttt tgattttctg ctgaataagg
    29281 ctgccagtcg ctgatgtctg caccaaatgg ataaatcaga atatttgttt ttcctaggat
    29341 aggtgcaact tcttgctgcc acaattcatt gtcttttttt acatcttcta gtgaagcttg
    29401 agtcatattc aagtgacccc atgtatggga agcaaagctc cagccgtctt tcttcaactg
    29461 ttccgccact tttttagctt ctttaatctc tttttttgtt ttttcattgt ctccatattg
    29521 agattttgat gttcgataac ctaaaactcc gttgtaacct gtcagggcca aggtaccttt
    29581 tgcgcctcga taagaaaaat caggatgttt cttgacaaag gcatcgatca gaggaaccat
    29641 gtcaaagtca ccgatttctt ctttatcttt ctctttatag acatttttga tttcattgtt
    29701 atttgatacg accaatcggc taggaaaacc ggaattatcc atatattcat aatagctgac
    29761 atcatcttgt gaaagaacca atggtttttt tccttcaggt agcgcaagcc cgttaaagga
    29821 aagatttcct tgttcgtctt tttggatcaa atcattcaaa ttaactaaaa caaaatcatt
    29881 ttgataaagt tgttcgatcg aacggttgaa ttcttcaaca gtcaccatat aatctttgta
    29941 tccttgtgcc tgttgagagt ggaaggcttt ttgcgggtcc acgatcaagc tgtggaaaaa
    30001 gacatgagag atctgagtag gatcagacca gtcgatcagt tgttctttct ccttttttag
    30061 tgtttgaaga agctgttgtg tttcagtaga tccgttctta ctatcttttt ccaataacgc
    30121 aatcgcttgg tcataatcgt agctttgagc cagatttttt gcttgagtaa ctagttgttg
    30181 cttatcatct gtcggttctt ttgctttttc tgttattttt ttttcaggaa tgattggttc
    30241 tttttttgta gatgatcgga tgacaaaaaa caaacttaga attaaaataa cgatagttaa
    30301 agcagaagtg gctcctaaaa taattttttg ctttttttcc atatacctcg tcccttgctt
    30361 tttcattttt ttacttgtcc cattccttat tgtaacgaag aaagaataag aggcaaagta
    30421 aagtgacttc tcgataaatt gcagaaaaaa tacagaaaaa gttatttctt ttaacaataa
    30481 ttgaaaataa aatgttcgga attagagcgg attcaaaaaa gaaaaaatga ggaatttaaa
    30541 aaaaatatcc tttttctgtt gacaaagaaa aagttgcgct ctatgataga cacatattaa
    30601 taataacaag acgaagatcg aaacgaagta gttgttaaag ccaatgtttc agaaagctgg
    30661 tggtcgctgc aaaccagtac atgctttaaa gcgaattaca tttcaggagt ctttggcagt
    30721 gattgccacg cgacagcgtt aaatgttcga gaggtatgtt ggatatctag acatacaaat
    30781 tgggtggtac cgcgagattt cgtcccttgg cttatatgcc aagggacttt ttttatttcc
    30841 tatcctatcc tgaagaaata attaattaca catgctagca ggttcctgct ttcataaaaa
    30901 aactacacgg aggtcagtaa aatgaatatt attgatgaat taacttggcg cgacgccatt
    30961 aatcagcaaa caaacgaaga aagacttcgc gagctcgttg aagaaaagag catttcactt
    31021 tattgcggcg ttgatccaac tggtgattcg atgcatatcg gacatttgat tccatttatg
    31081 atgatgaaaa gattccaatt ggcaggccat catccatata ttctgattgg cggcggaaca
    31141 ggaacgatcg gcgacccaag cggccgcaaa acagaacgtg tgttgcaaac gatggaacaa
    31201 gtgcaacata acgtggacgc actttccaat caaatgagaa aattgtttgg taaagatgca
    31261 aatatcacat ttgtgaacaa ctacgactgg ttgtcaaaaa tctctttact tgaatttttg
    31321 agagactacg gtaaaaactt caacatcaac acgatgttag caaaagatat cgttgccagc
    31381 cgtttggaag taggaatctc atttacagag tttacttacc aaatcctgca atctatcgac
    31441 tttttgcatc tgcataaaac atacgatgtg caattgcaaa tcggtggagc agaccaatgg
    31501 ggaaatatca cagctggatt agacctaatc cgaaaattag aaggaccaga agcagaagcg
    31561 ttcggtttaa cgatcccatt gatgctgaag gctgacggaa cgaaattcgg aaaaactgca
    31621 ggcggtgccg tttggcttga tccgaagaaa acttcaccat ttgagttcta ccaattctgg
    31681 ttgaatcaag atgatcgcga tgtagtgaaa tacttgaaat tctttacttt cttgtctcaa
    31741 gaagaaatcg aagacttagc gaaaaaagta gagacagaac ctgagaaacg tgaagcacaa
    31801 cgccgtttgg cagaagaagt tacaagattt gtgcatagtg aagaagactt gaaagaagcg
    31861 caaaaaataa cgcaagcatt gttctctgga aatattaaag aactaaatgc ggaagaaatc
    31921 gcgcaaggat tcggtaaaat gccgaacgtt gaaatttcaa gcacacctga aaatatcgtg
    31981 gaactgcttg tttccacaaa aattgaacca tctaaacgtc aagcacgtga agatgtttca
    32041 aacggagcta taagtattaa cggtgaccgc gttaccgatt taaattttgt cataaatcca
    32101 tccgatgaat tcgatggtaa gtttgtggtt attcgaaaag gtaagaaaaa ttactttttg
    32161 gccaaagtaa ttgattagaa tttagtaagt ttttaccttg aaaaagggaa atactattac
    32221 aacattccaa ttttgaaacc catcctaaaa tagaacgctg gttgtcgtta atacaatctt
    32281 tgcgagggag tttacatttg taaattccct cgtttttttt aattatttca aaagtgtgaa
    32341 caattcggat aattgataca tattaaaata tgattaaaaa tcaggccaaa acttagttta
    32401 cagaatagaa gaacaggtgt aatgtagggc tcgacaaaat ttaaatattt ttttaggagg
    32461 gtttcactat gagtgaatca ttgtcgaaag atctaaacct aaatgcccta ttcattgggg
    32521 acaaagctga gaacggccaa atctataaag ctttgttgaa tgaattggtg gatgaacatt
    32581 taggctggcg tcaaaactac atgcctcaag acatgcctat tattacgcca gaagaaaaaa
    32641 gcagtgctag ttttgaacac actgtgaata aaacaaagga tgttctatca gaaatttcag
    32701 cgcggatgcg tacccattcc gttccttggc ataatgccgg acgttactgg ggtcatatga
    32761 actctgaaac attgatgcca tctttactag cgtacaattt tgctatgcta tggaatggta
    32821 ataacgttgc ctatgaatct tctcctgcaa caagccaaat ggaagaagaa gtaggaatgg
    32881 aatttgcaaa attaatgagc tataaagatg gctggggcca catcgttgct gatggttctt
    32941 tagcaaactt agaaggactt tggtatgccc gcaacatcaa atcattgccg cttgcaatga
    33001 aagaagtgac acctgaacta gttgctggaa aaagcgattg ggaattaatg aacttgtcaa
    33061 cagaagaaat catgaatctg ttagacagcg tcccagaaaa aatcgacgaa atcaaagctc
    33121 actcagcacg tagcggaaaa catttagaaa aattaggaaa atggttggtt ccacaaacta
    33181 aacactattc atggctaaaa gctgccgaca tcatcggtat cggtttagac caagtgattc
    33241 cagtgccagt tgaccacaac tatcgcatgg acatcaacga acttgaaaaa atcgttcgtg
    33301 gtctagcagc tgaaaaaaca ccaatcctag gtgtagtcgg tgttgtagga tcaacagaag
    33361 aaggagcaat cgacggtatc gataaaatcg ttgcattgcg tcgcgtatta gaaaaagacg
    33421 gtatttactt ctacttacac gtagatgctg cttatggcgg atacggccgt gcgattttct
    33481 tagacgaaga caacaacttc attccatttg aagatttaaa agatgttcat tacaaataca
    33541 atgtctttac agaaaacaaa gattatatct tagaagaagt acacagtgca tataaagcaa
    33601 tcgaagaagc agaatctgta acgattgacc cacataaaat gggctatgtt ccatattctg
    33661 ccggtggtat cgtcatcaaa gatatccgca tgagagacgt tatctcttac tttgcgacat
    33721 atgtatttga aaaaggcgca gatatcccag cattacttgg ggcttatatc ttggaaggtt
    33781 caaaagcagg ggcaacagca gccagcgttt gggcagcaca ccatgtctta cctttgaatg
    33841 tcacaggata tggtaaatta atgggtgcat caatcgaagg tgcacatcgt ttctacaact
    33901 tcttgaatga cttgtcattt aaagtaggag acaaagaaat cgaagttcat ccattaactt
    33961 atccagattt caatatggta gactatgtat tcaaagaaaa aggcaacgat gacttagttg
    34021 caatgaataa attgaaccat gacgtatatg attattcttc atacgtgaaa ggaagcattt
    34081 acggtaatga gttcttaact tcacatactg actttgctat cccagactat ggcaacagcc
    34141 cattacaatt tgtgaaccaa ctaggattct cagatgaaga atggaaccgt gccggaaaag
    34201 taactgtatt gcgtgccagc gtaatgacac catacatgaa caaagaagaa cactttgaag
    34261 aatatgctga aaaaatcaaa gcagctcttc aagaaaaatt ggaaaaaatc tacgcagacc
    34321 aattattggc aagcgaagca aaataagcaa ggtaatcatg ttagaaaaca gcttgaatac
    34381 taactgtttc tgacgcatgt ttggatctga agagcaaggg gagacctaag ctcttcgatc
    34441 cctttgtttt gaggttgtct ttgaagaaac ccttcaagca ataaactcaa agaatcccct
    34501 caaatattcg ctgaattgtt tcgtttattg ctgaaactac ttcaaagaca ccgttatttt
    34561 atccaaaaaa tcaaggagag tgtcaacggt gagtaaatct gaagtaagta aaatgggtct
    34621 tgggaccttt atcggtctga caatggcact gtgtgcaacg gttcggagta ttcctacgtt
    34681 agccgcagtc ggttggacat tgatttttta ttcaattttt gcagttttgt tttttgccgg
    34741 tccgatatca atggtttccg gtgagctttc cacgatgctt cctcaagaag gcggtcctca
    34801 gttatgggta aaaacagcac tgggcagcaa atggggattt gtcgttgcat ggctattatg
    34861 ggtgcaaatg ttcccaggta tggtaatggt cgcttctact ttaggaccat tgttaggaaa
    34921 cacatttggt aatgtagaac ttggcaataa tcactggttc gttttaggtt gtattttagt
    34981 tatctactgg atcatcacga tcttgaactt gaaattcgat atggcaaaag tcggcgggaa
    35041 catcggtgta tggctagggg tatatatccc agtagtcatc atgttcgttt taggtgtttt
    35101 ggcagccttc aaagtcggac tagtctcaaa cggctatcta ggtgactttt catggtcaaa
    35161 agctttccct gacttggaac atattgattc attgaaatac ttagctggta tcacatttat
    35221 ttttgttggg atcgaaatga gttctgttta catgcctcgt ttgaaagacg caacaaaaaa
    35281 ttatacaaaa ggcgtattta tcgctttgat cggattagtt ttattgaatg tcatcaatgc
    35341 gatgcttgta gcaaatgtcg taccagatgg aaaaatggaa ttagcaaata tcacgcagcc
    35401 tatcttgatt gactgtcaaa tcctaggctt accagaagtg atcggaaata tcttcagctt
    35461 catggtattt attggtgtgc ttttacaatt atcagcttgg gttactggac catcaaaaac
    35521 aatcatccaa gtagctcgtg aaggattcct tccaccaaaa ttcggattcc ataaagaaaa
    35581 caaatacggc gtatctagaa atgtcgttct aacacaatca atcgttattt cattatttgc
    35641 tttgctttat ggtgtaatgg atgacgtaag tgctgtattc ttgacactta caaatgctac
    35701 aactgttatt tattgtatcg tgtacatttt gattgctgtt tcattgctta aaatgcgtaa
    35761 gaaacatcca gaattcgaac gtccttaccg aattggtaaa aacggaaacg gattagcttg
    35821 ggtagtcagc tgcatgctga tcttctctat catcgttgtc gtatttgcta cattaggtac
    35881 agcgacattg agtgatgcat tattagtagc agctatcaca gtggtcatgt tcgttattcc
    35941 attgatcatc aatcacttca agaaagattc ttgggggatt gaggtagaaa aaagtttaga
    36001 agaaaaataa aaaaataagt ctttcagccg acgttttgtc gctgatttag taggaggtcc
    36061 tgccaagtga atagtttttt catggcaggg ccttttcatt tgaaatagga gatcgacgat
    36121 gaataaaaat atttctttaa aagaaagtat cgctgtattg atcgtgttgt tggcactcct
    36181 aggagtactg atcatcggat tccagctctc gccacatatc ccgatccttg cagctttcat
    36241 gtgtttgttg ttttatggca gatttaaaaa gttcacatgg gatgagatcc atgacgggat
    36301 catcaaagga atcactccag ggatcattcc aatcctgatt tttcttttga ttggtgtctt
    36361 agttgcttca tggattttat ctggaaccat ccctacgatt atggtttatg gatttaaact
    36421 tgtttctgtg cgtttctttt taccgacagc atttcttgtt tgtgcaattg tcggagtgac
    36481 tgtcggcagt tcatttacaa cgatttcgac gattggaatt gcttttttgg ggatcggtca
    36541 tttacttgga ttcaatgatg caatgactac aggcgcagtc gtttccggtg cctttttagg
    36601 gaataactgc tcgcctttat cagatacgac caacttggca gcgggtattg gaggagttaa
    36661 tctattcgaa catatcgctg gtatgcgtta tacggatttc ccagcattcg tcctttcgtt
    36721 gattttttat acgatattag gacataacag tcattcagcc gatctagcta gtatcaatga
    36781 aatgatccaa aacatgaaag cgagcttttg gatctctcct tggacattga ttccactagt
    36841 tattttattt gggggggcaa tcaaaaaaat tccggctatc cctacattac tagcgggatc
    36901 gacagctgct ctcgttcttt cttttatcca tgagaaaagt ttgacggtcg gaaaagcagc
    36961 agacatcttg atgaatggtt atgtcgcaaa tacacctgat ccaaagctag atgaactgct
    37021 ttctcgaggc gggatcatga gtatgttagg ttctgctagt ttgattttac ttgcattggc
    37081 attaggtggg ttactgatca aatatctgat cgttgatacc atcgttcaag aattaaaaga
    37141 aaagatggac cgaccttctc gtttagtcgg gtttactgcc ttgagctgta tcggaatcaa
    37201 tctgatcgtt ggagaacagt atctgtccat cattcttcca ggagaaacat tcaagcgttc
    37261 ttttgatgac gttggcttat caaaaaccta tttgacacga acactagcgg attcaggtgc
    37321 gaccgtcaat tctctcgtac cgtggggagt cagcggcaca ttcattatgg gaacgatgaa
    37381 agttagcgcg ttgcattact tgccattcgc ctttttctcg attcttgcac ctgtctttac
    37441 aatattaggc ggttttttat tgaatcgtcg acgtgtaaaa aattgaagga aggaaaaaag
    37501 aggctgggac agaagcgttt aactccaaga aataagaaga aactcaagaa aattgctttt
    37561 taaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata
    37621 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct tttttacaga
    37681 taaacaatgt gttagaagta gtatcttctt ttacataaaa agctgaccat aggataaatg
    37741 gtcagcgtat ttagtgagtc agctatttag tatgggtttt ctaaaggaac gactgtctct
    37801 cttttgatca agcgatgagg gatgaccaaa cgtacgccac tgctcaattc ttcatggatc
    37861 aattcgcgca gcttttgcat agcgacacgt cctaattcgg agatatcgat atcgatactt
    37921 gtaaggtaag ggtgtgttaa tgttgagaag atcgagttgt tgaagctaat gacggagagt
    37981 gtctctggaa tgacatagcc atacatttgc gccaactgca tcgtgcgaag ggcgaagata
    38041 tcgtcaatga ccaccaatgc tgtggcttcg ttttctttca acgttttctc aaaggacaga
    38101 taatcctccg gatgctctag tactacactg gataacgcag gaagaccagc catcatcatt
    38161 gctttttgat aaccgaaata tcgttcgaaa tacaaacttt catgagtcgt gtttgtcaca
    38221 aacaggatat tcttatgtcc attctggatc aaaaattctg ttgcttgttt acctagaagc
    38281 tggttatcat tatctacata aacgatttcg ttctcatgat gataaggctg tccgatcaaa
    38341 gtgaaaggga tgtggttttg gtataaataa tcaatgacag tatcttgact atctgaatac
    38401 actagaataa aaccatctac ttgtttttgt aaatgcatac gctggacgtt ctctaacagc
    38461 aattcaaaat ttttagcagt agcgattgct gtagtcatat cataattccg cgcttcttcg
    38521 ttgattgctt ccatgatctc aagataaaat gggttaccga tccgctcttt tgaatcaaga
    38581 gggggtaaga ttacgccgat ggcactggaa atgcgttttc ctagattgcg ggcagtcatg
    38641 ttaggaacat aacctagttc atccataatt tttcggactt tcttctttgt tgtctcggaa
    38701 atactaggat gatcgttgat gacacgagag acggtcgagg tggcaacacc ggcttttttc
    38761 gccacatctt ttacggtaat agccatgata aaaacacctt tctcttgtta ttgtgcagag
    38821 aaccgataaa tcgtctgact gatccgttct tgaccaggat ggaggttgat cgatccaaat
    38881 ttggggaaat gaacgatatc agggatttct tgcggttcga ttgctaatcc gtagtttgaa
    38941 tgcatctgtt ttccattgac agagaaatca gcttcgaatc cagtcgtaga aaataatacc
    39001 atacttttat tagaagttgc tatagtcatt tggcgaccag aatgcttctc gaataattgt
    39061 aaggaagttt tggaaagcct aggatgatgc aaaagaaata catcatctag tccatctgga
    39121 taacatgcaa ggatctcttg cagtgttggg gattttctaa agtcgattgg cagtttttct
    39181 ttttttgtcc aatttccagt aggcagtttt tcttgatcta attcaagcac accatctaaa
    39241 aagaccgtta actgatgttc atagatgtct cttgttccat ttcctgaaag gttgaagtag
    39301 gcatggttgg tcggattgca gatggtttca acaaggctac tagcagaggt tgtcatctct
    39361 aattcgtttt ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg ataggtagaa
    39421 aattcatctt ttaagtaaaa gaccacacct attgatgatg gagttttgaa aacttctact
    39481 tcccaaaatt ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga tccgcagttt
    39541 ttctccaatc tatgctcacc ccaagaagca tcccggatcc ttccggctac aggtccgaca
    39601 gttgctccaa aaaagctttt gtctgttaga acatcttcga aacgatcgta agacaataaa
    39661 acattttctg attttccttc tttgtctggt gcgaagattt ggtgcaggcg ggcaccgtaa
    39721 ttgagaagaa cgacttctaa gtagtcgttc ttcaatgtga ttttctttat ttcagtctct
    39781 ggaactggta cgacggagat ctctggatta tccatggtca tgccactttt ctttcaagta
    39841 atcgatcgta agaaaagtcg tatcagggac aagcgggatc tcctctccaa ggtcttcagc
    39901 acgtcccacg ccaattggtt gagcgccact tgagaggatg gcttggatac cggcttgcgc
    39961 gtcttcgatc ccgatacatt cttctgcttt tagtccgaca gcttttgctg caaggaggaa
    40021 gatatctggc gcaggttttc cgtttgttgc ttctgccggg tcggcaatag catcaaagta
    40081 aggtgtaaga cccatttttt caagtaggaa tggcccgttt ttacttgcag aagcaagagc
    40141 aatcttgatt ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg ggaagacatc
    40201 ttttggttcg atcgtctgga tcatctctaa ataataatcg ttttttcttt tcgctaattc
    40261 tgcaaattct tcctttgaaa aagagtgttc ttttccgcca taagctaaaa gcaaagagag
    40321 tgagtcttct cggctgacgc cttttagctg ttcgttgaat gtgtggtcga tcgtaatgcc
    40381 gatttcctga ccaagctttc gccaagcacg ataatggaat tcagcagtat cggtaatgac
    40441 accgtctaaa tcaaacaata cccctttaaa catttgcttc ctcctttctt agcgttattt
    40501 ctagacgatc cgctaaagaa taagactgtt catagacgta aatttctaga ggagaacctt
    40561 ctaataactc gaaagtaact tgttcatctg tgactgagat gaacaataaa cgtccgcgat
    40621 aattgatatg gaaagaataa gctttccaag tttttggcag aaatggtgcg aatctcaact
    40681 gatcatgggc agttttcatt tgagcaaatc cttggacaat cgacaaccag ctaccagtca
    40741 tggaagtgat gtgcaagccg tcttctgtgt cgttattgta attatccaaa tccaagcgag
    40801 ctgttcgctg gtacatttct acagctttgc tttccattcc taattctgct gctaaaatcg
    40861 aatggatact tggcgaaagg gaagactcat ggacagtcat tggttcatag aaatcaaagt
    40921 ttttttgctt ttcttctttt gtaaaagtat cattgaagaa ataaatcccc tgtagtacat
    40981 ctgcctgctt gatgaagcaa gatctaagga ttttgtccca agaccaatgc tgattcagag
    41041 gaagatcgct tgtgggaata tcggatacag gcatgagatc tttatctaag aatgtgtcat
    41101 gctggacaaa gattcctaat tcttcgtcaa ctgggaagta catgttctct gcgatatctt
    41161 tccatttggc tagttcttct tcgctgatag tgatagcagc ttctttttga taagttaaat
    41221 aattttctcg cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac caattgttat
    41281 tgatattgtt ctcatattcg tttgggccgg tcacaccgtg gatcatgtat ttatttgctc
    41341 gttttgaaaa atggacacgg tctgcccaga agcgtgcgat ttctgctaaa acttccagtc
    41401 cttcattttt caaatattcc gtatcgccgg tgtaattcgt ataattgtag attgcatagg
    41461 caatcgcgcc atttcggtga atctcctcga atgtgatttc ccactcgtta tggcattcga
    41521 caccagtgaa ggtgaccata ggataaagcg cgccttttaa tccttgttgc tgagcgttgt
    41581 ggattgcttg cggcagttga ttatgccgat atttcagcag attcttagtt acttcaggtt
    41641 tggctaaagc aagataaaga gggacagcat acgcttctgt atcccagtaa gtggctccgc
    41701 cgtatttttc tcctgtgaat cctttggggc cgatattcaa tcgttcatct tcgccgtagt
    41761 aagtagagaa tagttggaac aaattgaatc gaataccttg ttgtgcttct tcgtctcctt
    41821 cgatgaccac atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc tcttcttttg
    41881 cttggatata attgtttgtc tggttcgtta attctgagac agctttttct gcttgctgcg
    41941 cttctggaat atctctacta gtaagaacga taatttcttt ttccactgac catgtctcac
    42001 cttgagataa ttctgcagac aagcaaccat gtaatgctaa agtatcttcg tgatagtcag
    42061 ggatgactga ttttccatta acgaaatggc gcattgctgc agtaacagta aattgttcaa
    42121 tcccaaagtt gtttggaata gttttagtcg tcaaatagcc gatttcttct atagttccac
    42181 gatcaatctc attccaaaaa tgttcatcat agttgctgtc ttcgttttga acatttccat
    42241 ccaattttga tacgagtttg actgttccgc taccttcaag catttctata gttaaataga
    42301 tataagctga ttcttttttg acaatgctca gaaaacgttc gaaggaaatg cgtactttat
    42361 tagtagaagt agtgattgtg aatgtacgag ataatacacc atttttcata tctaattccc
    42421 agtaaaagtc ttctggttcc aaagaagcca agtcgatcag ctgatcgttg atcgaaatat
    42481 ctatcgcgat aaagttcaag gcgttgatca cttttccaaa gtattctgga taaccatttt
    42541 tccaccagcc gacacgtgtc ttatcgggat accagacacc cgccaaataa gtaccttgat
    42601 ggtggtcccc tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa ccgtttccaa
    42661 tacttgttaa agattcttgc agtcgaagat tttttttatc taattgtgtc gtgctgattt
    42721 tccatggatc aatagcaaat agtcgtttga tctgtttcat ttttctcctc ctaatgagct
    42781 gttttatacg ttcataataa acgcaaccga ttgcacaagt caatagacaa aagaaaatat
    42841 ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa gggaacacac
    42901 gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc atttacaaaa
    42961 aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag ctaaacggaa
    43021 tgaaaaagat atttagtttt gagttttggc aaaaatttgg aaaggcttta atggttgtgg
    43081 tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt cccttgatca
    43141 atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt ggttgggcaa
    43201 ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg gcaaaagaaa
    43261 gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg atcactgggg
    43321 ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc cacactctgt
    43381 tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca ccagctttga
    43441 atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct tataataagt
    43501 actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag cgctttgttc
    43561 cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg gtgtggccaa
    43621 acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa gaaagcgcac
    43681 cgattctagc accgttttta tttggaacat tagaacgttt actattgcca tttggattgc
    43741 atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat gagattttat
    43801 caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg gcttgggcca
    43861 ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt gtgttgcata
    43921 actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt atcttgatgg
    43981 gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga tataaatcaa
    44041 tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca ttggagttca
    44101 tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa ggggctgcat
    44161 ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc gaattgctga
    44221 ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac ttcgtgttgt
    44281 gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc aaaaaattca
    44341 actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa accgttgtat
    44401 ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat ttgctgggag
    44461 gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta agtgtaaatg
    44521 atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg ggattgatcg
    44581 tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg aaatcagata
    44641 tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt tctacaggaa
    44701 atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt tccgcagctt
    44761 ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa aaaatgatgg
    44821 gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc gctggaaaag
    44881 tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat ggtatcgaag
    44941 ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt gagattgctg
    45001 tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat cttgatgtga
    45061 tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat aaagtagaag
    45121 aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc tccattaaaa
    45181 tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag ccaaacaaaa
    45241 atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca gtttgaatac
    45301 tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa cagccaaaca
    45361 aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga acactatttg
    45421 ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg aagacaagga
    45481 gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca gaaccagaga
    45541 aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg attgctttac
    45601 aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta atgaagttct
    45661 gtccggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt ttagttcagc
    45721 ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac atcggttatg
    45781 ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt caggtcctta
    45841 ccgtctccca atttcaaaag aaagaagatt atcagacacg caagatcttg attgggaaaa
    45901 caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg tggacagata
    45961 aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg gaaactcgcg
    46021 taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt tattatactg
    46081 taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa aaaggaaaag
    46141 cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa ttgcgtatag
    46201 attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt tttgatggga
    46261 tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat gtgaacgaat
    46321 aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca aacgattata
    46381 ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca tgtaaaagct
    46441 tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa gtaagagaaa
    46501 gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg attccttttt
    46561 gctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat gtttttccat
    46621 tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt ttgtccccag
    46681 tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa gttgtttcgt
    46741 tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc ttatagattg
    46801 gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata tctttgctga
    46861 agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga ctgacaaatg
    46921 tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt gctgaaaaaa
    46981 taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac tggttcagtg
    47041 aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga aatggaaact
    47101 ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga atcaagctag
    47161 tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat aaggtgttct
    47221 tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga aaggccttag
    47281 ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt tctctgttaa
    47341 caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc caaatatttg
    47401 ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct tttccttttt
    47461 cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct aacatgatcg
    47521 aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg ataacaggga
    47581 actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa aaacaagtcc
    47641 cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta ctcatgaata
    47701 aaacggctgc tggtacactt acccaatttc gaacattgac atatctcagt aaaatcagca
    47761 tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa tgaaaccagc
    47821 tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg taaacggcat
    47881 tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg aaatccccat
    47941 atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca tcagcaccca
    48001 aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg aaaaaaattg
    48061 ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta gataaattca
    48121 tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ttcatgaaaa tatttatgaa
    48181 ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat aagttttgtt
    48241 gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta gctttttcat
    48301 gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca ctagcctaga
    48361 aaatatgaga agctattttc tgactagcgc tcttattact caaagcataa cgcagttttc
    48421 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaacccaa
    48481 caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca ggacagcttt
    48541 ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata ataaaagaaa
    48601 gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa acagtctcgt
    48661 cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact gatcgcttta
    48721 tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat cgaacaagta
    48781 caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga gaaaaaccaa
    48841 cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa gaacagaaat
    48901 gaaagtacaa catccagcca attagaggaa tggctgatcg gaagtatcaa tatacctaag
    48961 atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga aaatggagca
    49021 ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc agtgatctct
    49081 gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt agaaaacgga
    49141 gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa aaatatacaa
    49201 gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga cttagttacc
    49261 ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac aggacatcgg
    49321 attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga aagaaccatg
    49381 cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt agtgtttatc
    49441 ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc atttattatt
    49501 agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca taagaaaaaa
    49561 acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg gaaagtcaag
    49621 ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc taaagttgct
    49681 gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga ggggcgaaaa
    49741 cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata agtaaggaga
    49801 gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc tgttctatcg
    49861 atttggctgc atgctaactt ggattctcat gaattcattc catcaactta ttgggagaat
    49921 caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc agaaatagat
    49981 caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat ctttgtgaaa
    50041 agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa agaaaccaat
    50101 aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt ctatctttca
    50161 caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga ggaacttgtg
    50221 atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa tgctcccagc
    50281 ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact gtgtgaatat
    50341 gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt attagctcaa
    50401 aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc aggtcaaaac
    50461 gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg taataagccc
    50521 actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac tcaaagcata
    50581 acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc ggtattagct
    50641 caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata ctcaggtcag
    50701 aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata attttcatcg
    50761 aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc gctaataaca
    50821 tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc aggaacaagt
    50881 aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat atgttcgttt
    50941 tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa gatcattccg
    51001 tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt tggctctgtc
    51061 caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg gtcttttacc
    51121 caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc atttttggca
    51181 taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc aataaaatct
    51241 tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa catgtaataa
    51301 actacatgtt ttcgattact catttatgat agaatggatt taaagatttg tcaaggaggg
    51361 aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat taagtaagaa
    51421 gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag aattttggca
    51481 agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag gaatcattca
    51541 gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat taacagaaga
    51601 agggaaggaa caattggcag agtggattca tacacccacc aatgaactac cggttaatcg
    51661 agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg cattagtaga
    51721 aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat tagagcgaaa
    51781 aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact acctgatttt
    51841 agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg tattagaaga
    51901 aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct ttctatatga
    51961 aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt ttcaagtatt
    52021 tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg tttagagttc
    52081 ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt aagttaattc
    52141 gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga agttaccgat
    52201 ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt ttgaaaaaac
    52261 tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag cttttgacaa
    52321 agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg taggtaaacg
    52381 atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct tttgttccca
    52441 ataccttaat tgacgtggag acacacctac gatatcactt aattcactga tgccaacaac
    52501 caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg tcactccttt
    52561 tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta ggttgacaaa
    52621 tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa agaaggagaa
    52681 atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg acacttttag
    52741 ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta ttgacgacag
    52801 cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa tggttaacga
    52861 ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg atcaataaat
    52921 tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga acgattactt
    52981 gctttacagc acctaatttt agtacattac taatcggtcg tttgatccaa gcttgcggtg
    53041 tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg ccagaaaaaa
    53101 gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct ttaggcccta
    53161 cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt atggtactgc
    53221 caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt attccattgt
    53281 caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc ggtagtttac
    53341 tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta attggtttct
    53401 tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat ttggaacatc
    53461 cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct gttttagctg
    53521 gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat attcaaaata
    53581 ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca ttgatcatgg
    53641 gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa cgtttagcca
    53701 tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg acaaaaacga
    53761 ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc tccatggtca
    53821 tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc agtcatggga
    53881 cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct attttgatca
    53941 gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg gataatacgc
    54001 cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat gccgcgttta
    54061 tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg aagaaaaaag
    54121 aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga ttgtcagctt
    54181 gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct ttttatcttt
    54241 gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt tgtccaatca
    54301 ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca gtgctgattc
    54361 ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa aagtgtatct
    54421 ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata acgaaacaaa
    54481 taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt attgggaata
    54541 caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg aatatgacag
    54601 ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg accaagcagc
    54661 caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag caaaagctta
    54721 tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca aagcagagca
    54781 agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg caaaattagt
    54841 gaaagaagca aaaggcgaat aatattttga acaccgttta aggaagaggt tgtgagcctg
    54901 ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca gcttctcatg tttgttgact
    54961 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcgaaa aaaggaactg
    55021 cgacaagact tttgtcacag ttccttcttt tttttgctat gctatactag atgtaaaaaa
    55081 taggagaaat aaaatgaggg aaatttattc ttttgaatca aaaatctatg cttccgaagt
    55141 cggcaagggc ggggcatata ttatttttcc gtatgatata agaaaagaat tcggtgctgg
    55201 aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat gaaggcagta tcgtgaatat
    55261 gggcgtgaaa aatccagatg gttcagtctg ctacatcctt ggactacgaa aagatattcg
    55321 taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt acagtgaagc aaaaataaaa
    55381 gaggttgtga caaaagacgt gtcacaacct cttgataaac ggtgttcaaa agccagtttc
    55441 tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca tgtttacaaa atttttctta
    55501 ctactcgaaa cgaagcggct ttatcacaac ctcttgataa acggcgttca atcagctgtc
    55561 cctcggtatt agctcaaaaa cggcaaaata tgagaagcta tttttccatt tttttctctc
    55621 atactcaggt cataacgctg atttcacaac ctcttctttc ataatggttt ttcaccatct
    55681 ttataataaa ctggttcttt gatatggaaa tctatttttt ccaaccaatc taaaatcttg
    55741 taacctaact cttctgacat cggaaaagag gcgttgattt gtgcattgga gatatggtca
    55801 acatggatac cggaagtgat cgtacaactt cccggaacgg cagttttgat gatcacagct
    55861 attttttcgg ctaagacatc gtctttatgg aaccgaccat cgtggctagg gaaacgtaca
    55921 gtttgcatct ctgtatcacg agaaaatgta gtgactgttc cgatatgtgg tgtatctccg
    55981 cctgttaaag tgatcaataa gtctttaccg attactttaa cagcagcttg gatcgtgaaa
    56041 cctagtttgg ttacttcaac tatcgtttct ttttccatat taatcaccct gccaacgttt
    56101 tgaatgctca tttacaattt caaattgatc caaaagtttc attgtatggt gattaacaag
    56161 atcttggatc gtttgaggct gattataaaa agcaggaatt ggtggtatga tttgcacacc
    56221 tagtcgtgct aatttagtca agttttccaa gtgaatggca tttaatggcg tttcacgagg
    56281 aacaatgact aactttcgtt gctccttcat tgttacatca gcagcacgtg caatcaggtt
    56341 gtcgcctaaa ccaacagaga tacctgcgac tgttttcata cttgccggta cgatgatcat
    56401 tccatcggtc aaaaatgatc cgctggcgac agcagcacct aaatctttgt ttgaataatg
    56461 gtaatcaaac aggctttcga attcagttag tgataagtcg gattccagct ttaagttctg
    56521 ttttgcccat ggactgatga ttccgtgtgt ttctatcatc ggatattctt ttagtttctt
    56581 cactaggttg atggcataaa tggtgcctga agcgccactt acaccaatca cgattttttt
    56641 cttccgttgt gccaattgtt ccattttccg cactcctttt tatgatgtca aaacagttgc
    56701 agatcattca ttatttcaaa tatttttccc aatcttctac ttcttggaat tttgcacgta
    56761 caaaatgttc tttcatatcg aaaggaactg taccgtcaaa gatagttttt gcacttatcc
    56821 cacggaaacg aatagagcca ggattatatt ctggtaattc tgatgggtcc aatggatggt
    56881 tcctcattcc tgggatcacg atgatatctt tatctccttg gaaacgagtg ttgaaagtcc
    56941 aaactacatc gttcatgtcg aaaatatcaa catcttcatc gactaaaatg actgtcttca
    57001 attctttgaa ggctgataaa gcaagaatag ctgcttgacg ctgaatacct tcatccgctt
    57061 cgttttcttt acggatttgc atgatggtca tcaatttacc accacctgca ggaggattgt
    57121 agacatttac tacttttcct ggaatagctt tatcgaccaa ggcaagaata cttgcttctg
    57181 taggaatacc ggccatagat acgtgttctt cactgggacc aatcgttgtt tgcatgattg
    57241 gattgtcttt tcggtgagta actgctttga ctttcactac ttgtacagct ggattagcat
    57301 ccccagtata gccaggaaat tctggcattg cttttcctga gttcgtgttg atatcttctt
    57361 gcattgtttc atttggcatg atttctgctt caatgatgaa ttcagcacga gcaataccca
    57421 cttcatcgac tgctatacca tcaacgattt gaataggttc gttacgcaaa gctcctgcac
    57481 accatagttc attgtagcca agtggagtgg taggtggttc gaaagtcgtg ccaatggcaa
    57541 ttgctggatc taaaccaata ttgatagtga taggcattgg tttattcaag gcttcgaatt
    57601 gtttttggaa gtgaccaatg tggcgtccgc caggcatgat gtacataccg atcgtatctt
    57661 tatcttctag aaccatacga tggatcgtta catcgctcat tgttttttct ggatctgatc
    57721 ctaagactac acccattgtg atgtatggac cagcatcttc aggagtattt gtaggtgcag
    57781 gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag atgaacgact tcttgtgctg
    57841 gagcgtctgc tttgtctact ttcacaggtt tgattggatt ttctacagcg tctttcaaga
    57901 aacgaccaag tgttcgataa tcatggtgca aaatttcacc tacacgttta cgactagcca
    57961 ttgttccgat gttcacacgt gtgccgggga aacctttgat attgttgaaa gtcatagctg
    58021 gtccttcttg agtcggacgt tttactgtac cgccagcacc gatataacgg taaacaccag
    58081 ataattctgc ttcaggatcg atttcaatat ctgtttcgtg atattgacct ggaagtgttt
    58141 ttagttcttc gatgactttt cttaaatcat acggttgatc tgtcatgaga gattcctcct
    58201 tagttgtctt tacctatcta tagtaaaagg tgaaaaagag gaaaacaatt caacttgttc
    58261 ggtaaagtat tctacaatgg aatggatgcg tttttttgat aatagaataa actatgatat
    58321 acttatcact aaaagaaagc gcatacttag tgttggagga aggaaagtga aaaaaatgga
    58381 ggagtcattt cctaaagcag taaaagtgga aaatatagcg aatattttga aagttacatt
    58441 tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca gaagagataa ccgatgcctt
    58501 gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt ctcgcacttt ctcgtaatat
    58561 gtggattggg actgaagtaa cgattgaagc agatggaaca gtattcataa atggaaaaga
    58621 caggtatacg ccagaagagt tgtggtacaa aggaaagaaa agtataccag aattataaaa
    58681 aataattaaa aaaaaacagc gcgaaacaag gaacatatta cttcctcgtt tcttgctgtt
    58741 ttctactatt tcagcgctct tatgactcaa atctaaatgt ggttgtcaca accttattct
    58801 gcacgcaaag cgatagcggc gtcttttttc gctgccattc gtgcaggaag atgaccgcca
    58861 agcatcgtca atatagtaga gacgatcact agaataatcg catggacagg atttagttga
    58921 gcaacatttt ttagatccgt catgttgtat aagatgctat tgatagggaa cgtagctagc
    58981 catgcaataa acacacctaa tatcccagaa gaaataccta aaatacacgt ttctgcgtca
    59041 aatacgcgag tgatgtcttt tttacgtgca cctaaagctt ttagtacacc gatttctttt
    59101 gtccgttcaa tcacagatgt ataagtaatg atactgatca tgatcatact tgttactaaa
    59161 gagatccctg caaaagcaat aaggacataa gtgatagcat ccatcaatcc gccagtcagt
    59221 tcagtcatcg ttccagctaa atcagtataa atgattttat cttcatctga tttgccttta
    59281 ttgtaatcat ctagataatc caagatcttt tctttgtctt caaaattgtt cggataaatc
    59341 atgatacttg aaggcaagct gtctccacct aaataactaa gcaatgtttg tttggcattt
    59401 tcgtcaactt tttcagtagt taagacattt acaccactgt ctctttgagc tttgacgatt
    59461 tctgattctt tgttttcgtt gacgatttga gtcgttaatt gatcgctata agcgattcct
    59521 ggagataaga gattcattgt agaagaagat ttcacgcgta ggatccccga gatcttcaat
    59581 tcatcagaag cattctgata catcgcatca taatctgtat tagggataaa attcccagtt
    59641 ggaagttttg tatagtaagt attgttgttt actaatttga atgttgtacc aacgatatca
    59701 tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa gattcttcaa tgcgttgata
    59761 ttagtattat tattgccgtc tacgatcagc acgacatctg ttgctgaggc tggataactt
    59821 ccggcaagta aagaataatt gtcttttaaa aagttttctt tgctagtgtc aagctgtgta
    59881 gggaaacttg acactcccac acctgtcatt gctgacatcg tactagataa agaaagagat
    59941 tccgtatcag gattttgatt cgagaagctg acaggctgta ctttgccatt tacatcacgt
    60001 aaaagattga ttcctgtagt acgggtaaat ccgatattat tacttaagtt aggatcgatg
    60061 tctgttacgt aatcgacgta tgtttgatca atcttattcg tgtgctgtgc tttatcttct
    60121 tcgctgactt ttgcagtaat cgttttgctg tctggataat ccgctttgct tgcacccaat
    60181 ccggcatcgt cacgagtctg gttagtcgtt accttagaaa tagtaatcgg aaatttcgcc
    60241 atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc cagaagaaag ggacagaacg
    60301 atcgcgattc cgatgatccc aatactggaa gcaaaggagg tcaagaaagt acgtcctttt
    60361 tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc aaaaactcat ctttgttctg
    60421 cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag gatgggaatc tgttaagatc
    60481 ttaccatcag aaaattcaat gattcgatct gcgtattgat gggccagttc aggattatgt
    60541 gtgaccatga tcactaattt ttctttcgat aactcttgga tcaattccat gatctggata
    60601 cttgtttctg tatctaacgc tccggttggt tcatcgcaca aaaggatatc tgggtcattg
    60661 gctagtgctc gagcgatggc cactcgctgc atttgtccac cagataactg atttggcttt
    60721 ttatgcatat gatttgtcaa gccgactcgg tgcaatgcat cttctgcccg ttttcgtttt
    60781 tcgtctttag aaacaccact taatgtcatc ccaagttcaa cgttttcaat aatgcctaga
    60841 tgaccaatca agttgtaact ttgaaaaatg aaaccgatag agttgtttcg gtaggcatcc
    60901 cagtcactgt ccttaaagtc tttagtagac tttccgttga ttaccatatc accggaatca
    60961 taattatcta gtccaccaat cacattcagc atagtcgttt taccagaacc actaggacct
    61021 aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca caccatccaa tgctttggtg
    61081 gtggtttcgc cgactttata atattttttt atatctttta attgaagcat aatcaccctt
    61141 ctcccgtatt gaaatattcg gtatactaat aagtatatca gaacaatgat cgtagtatac
    61201 gaaataaatg ttaactgaag atgaactaca agaaaaaatc tagaaatttt tcaaaaaaca
    61261 gagggagatc aatgaatcgt attttagtag tagaagatga tccgcaatta cagttgcttt
    61321 accgttctgt gttggaacga gcgggctttt cagtcatcac cgcattgaat ggagaggatg
    61381 cgctgaaaaa actagaagcg acacgtatag aattaatcat tacagatatc atgatgccga
    61441 aaatggatgg ctatgacttg ctagactcat tacgtagcac cagatttgac acacctgttc
    61501 taatgattac ggcaaaggcc gattttgagg ataagaaacg cgggttcaaa ttaggagcag
    61561 atgattatat gacaaaaccc atcgatgtca atgagatgat cctacgtgtg gaagcattgc
    61621 ttcgacgttc aaagatcaat tacagcagta gcttgataat cggacaaaca aaattagagc
    61681 tagatgctta tacagtgtta caagatcagc attcgttgat tctgcctcaa aaagaatttc
    61741 tgcttctgta taaattactg tcttatccca acaagatctt cactcgccaa cagttgatgg
    61801 atgatatttg gggattagat acgaatacag aagaacgaac gatcgatgta catatcaaac
    61861 gtttaaggac ccgttttgaa aagacggaag attttcaaat catcacagta cggggactag
    61921 gctataaggc ggttatttaa agatgaaaaa aaagctattt tcacaattat ggatctattt
    61981 tgctagtatt gtcttcattt cgatcttagt gacactgctg tgttttctcg gttttatttt
    62041 cttattgtct cgacattcta tctcgcctgc tgaatcggct ggaaaaccaa cgttataccc
    62101 gttattcgtc tttgcggggt tcagtatggt cgtgggtaca ggaatatcga tttttgttgg
    62161 gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat atgagtctgg tagcaacggg
    62221 agatttttcg attcgaatgg atgaacagca gaaagtagca gaagtacagc aactgtataa
    62281 agatttcaac gttatggtcc aagaactcaa tagtatcgag acactgagga atgactttgt
    62341 gtccagtgta tcccatgaat tcaaaacacc actagcaaca atccaaggat atgttcaatt
    62401 gttacaagcc ccaaatctct ctgacgaaga acgccaaata tttttatatc ggattatcga
    62461 aagtatcacg cagctttcac aattaacaga gaacatctta aaattgaaca aactggaaaa
    62521 ccagcgtatc caactggaaa agaaagagta tcgattagat gaacaaatca gagaagtaat
    62581 cgtttttttt cagcctaaat gggagaaaga acagctagaa ttggatatcg aacttgctgc
    62641 agtaaattac acaggcaatg aagagttttt gtaccaagtg tggctgaata tcatggataa
    62701 cgccataaag tacaatcaaa tcaatggaca gattcatatc aaactatttg agacagccac
    62761 agagattgta ctggaagtga ccgattcagg tgtaggaatg aatgaagaga ccagagatcg
    62821 aatgtttgaa aaattttatc aaggagatac aagtcgtcaa atttctggga atgggctagg
    62881 actttcactg gttaaaaaaa ttctagaact gcatgatgga cgtattgatt atagcagtat
    62941 cgaaggagta ggaacgacag caatgatacg gttaagtaaa caataggaaa aagaataaaa
    63001 cccctaaaaa ttctattgaa ggtttttagg ggtttttagt cgtttttttg tgattaggga
    63061 aatgttctta cggttctttt ggcataaaaa tcgctgcttt cttattgaac tacgagaaat
    63121 gttagaggtg tcattgaaac aaggacatga tgcaagaaag agagcgaaag tcatgcgtcg
    63181 ctctcttttt ttgggtggat agatggacag aaaatgtagg gcttagtttt cttcttgatt
    63241 taaatactcc caaaatgtat ctgaagccaa agaatgctca ccgactttac gagtaaatgc
    63301 aagataactc tctgttgttg gagaaatagg gagaatcaca gtattttctt ttaatagatt
    63361 cttgactgtt ttttccataa caatagcaat ccccatgtca tttgcaatca tattgatgat
    63421 caagtcgatt cttgctcctt tataagagat ttttggttca aatccagctt cgcgacacag
    63481 atctagtaca ggttgataca agttcgtagt ggggccaagg agcaaaaagt tctccttttt
    63541 cagttcgctt agatccaatt tctttttact agctaaagga tgatttttag gtagaactgc
    63601 tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa cttggtgggg cctggaaact
    63661 tctagcaaag tggatcgtgt cactatgttc attggcgtca ttgtaagtta attcgacact
    63721 ttccacttct ttcaactgga ctgtaaattc aggatggcgt ttcaaaaact cggtgatttt
    63781 ggaaaaacct ttataattga tcattgtcgg tatcgttaag atatttaatg aaagattgtt
    63841 gtctttcgta taggtttcaa gcacttcttg catagcatgg tattgttcta cgacattttt
    63901 tacatatggg agcaataagc ttccagcttc ggtcaattcg atttttctat gggcgcgttc
    63961 gaataatttg acgtctaatt ctgtttctaa cgaaagaatc tgtttagaaa tatttccttg
    64021 ggtggtgaat tgtcgatcag cagttttggt gaaattcatt gtttcggcta aatcaagaaa
    64081 aacttcgaat ttatggatat ccatcatatt cctcctactt attccaataa agaatacttt
    64141 aacacaaaaa atgaattggt gtaatcttct ctgtctgtta caatctttct tgcagcgaaa
    64201 gcaataatta cagaaatttt tctgattttt ttcgcttcgt agaaaaagag aggagttttt
    64261 tgatgccaac tggagttttg atcaatgtag gttccgtttt gtttggcgga ctaattggcg
    64321 gattagtagg aaataaatta agtgaacatt ttaaagcaca gctgacgatg gtttttggtg
    64381 tgtgttcgat ggggatggga atctattcca ttgcgccgat gaaaaatatg ccggctgtta
    64441 tttttgcttt agtgatagga acagcgattg gtttgattgt tcatttaggt aatgggataa
    64501 ataagggggc tgcgctgatg caagtaccta tttctaaaat cttccctagt gaaaagcttg
    64561 gtatgaccca cgatgaattt atcagcacat tagtgacggt cattgttcta ttttgtgcaa
    64621 gtggtacggg gatctatgga agtttagatt ctgggatgac aggagacagt acgattttga
    64681 tttctaaatc tgtccttgat tttttcacag cagctatttt tgcgtgtaat ttagggtatg
    64741 tcgtttctgt gatagcaatt ccccaattca ttatttttta tattttgttc ttattggcaa
    64801 aatttattta tccattgact acaccagaca tgatcttaga ttttaaagct tgcggcggat
    64861 tcttgatggt cgctaccggt tttagaatga tcaacgtgaa aatgttccca gtagctgaca
    64921 tgatacctgc aatgatcgtt atcatgccac tgagctggat gtggacaaat tggattatgc
    64981 cactgttata ataaaaatca aagagaagcc attttttgct gactaaaaag gatcaaatct
    65041 tttagtcagt ttttttgtat ttgacaaact gattttgaaa ctatattata tgttgtataa
    65101 tatagattga aataaaatat tacacttgag aatagaaggt gacgtaatgc acatccagat
    65161 tccaactgtt cttcttgatg gtactgtgct agcagcatta agcaaggaag atatgtatgg
    65221 gtatgcctta acaaaaatcg tacaagaaag tctctctgtc agtgaatcta cactatatcc
    65281 ggttttgaga agattgaaaa aagaaggata tcttgagacc tatgatgaac cttttgaagg
    65341 cagaaaccgt cgatattact cgttgacacc aatgggaagt cgtcatttag aagaaattat
    65401 tgatgaatgg attgtttttc gagattcttt agaccggtta ttggaggaga gcacaaatga
    65461 atgaatattt gaatgaagtt gtggatcatc ttcaagaaat tcctgaagac gatcgttttg
    65521 atttgatcca gtattatgag gaatattttt tagattctgg aaaaacatta gatcagatca
    65581 ttgatgaata tggtacacca aagcagtttg ccttaaaatt aaaaatcagt tacttttcag
    65641 aagaagaaca agcagaaaac gagttagtga aaaatccaaa aagacaaatt cgcctgattt
    65701 ggttaatcat tatcggttta tttgcttctc ctgtcctcat tccactagca ctggggttta
    65761 tattggttat tgggggacta ttggttgctt tactagcgct catttttagt atctatgttt
    65821 tatgtatcag tatcttaggt gttggcctat tcatgatcat ctcaggaatc agtgtgtttg
    65881 gacaatcgat tctgagtgga ttattcttta tcggtgtcgg tctgttcgcc acaggaagcg
    65941 cagtatttat ttcgccactg ttgctgaaag gaacaaaagg gcttttttat gtcatgatga
    66001 actttgttaa atggatggga cgacgtttta ttacaaaaag agatctgcat tcagcgggcg
    66061 tttgaaggag gacacataaa ttgagattga aacatttttt gattaccggt attggtctga
    66121 tgcttactgg tggcctccta gcaggtgcaa cttatacatt aggcgctcaa aagagtctga
    66181 cttgggataa tggtcctcgg ttgatcgata tggttactga gacaaaaaaa atcaaggatg
    66241 caaacaaaat cgtcatccat gcagaaaatc aaacagtttc ggtcatacgc ggattggatt
    66301 ttgagataaa aacgacttat gataagacag aaaagccaga aataaaggag aaaaatggcg
    66361 tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat cattagttta gaagacacaa
    66421 gtgcacaaat cactattgtt gtccctgcag atcatgaatt atctgaattg acaatcgaag
    66481 ggaataatag tgcaattgat atcaacgaag tatcaatcaa aaaagtgaat actaagacac
    66541 atgatggctg ggtatcgttc aatgaattta caggaggaac aataactgcg aaagatgaat
    66601 taggctcact tcgattagat gaggccaatg tggataactt gatcgtcaca ggtgaaaaca
    66661 gcaacttcca tctagaagac agtttgatca gcaaagaagg aaaagtcatc ctaaacggtg
    66721 gcaatttatc tttgcatgag atggaaaaca gtggctatac tatccaaacg tctggtgaat
    66781 cttatattga aaaagactac cagccaataa caaatacttt gttagaacaa ggacagaaaa
    66841 agctaaagat cactgctaaa gacacatcga tcaatattac gacggatcaa gaggacaatg
    66901 aaacagattg atcctgtact caactggata ttttaagaaa aaacagcaaa gatccttcca
    66961 atctttgctg tttttcatcg cttaacgcac ataactatag taaaataaga aagaaaatat
    67021 ctaaaaagaa aacagggtga aagagtatat gaaggtagaa gaaggtcaaa gagaaaagtt
    67081 aaaaacagaa atggaaagat tacatactta tatcactcaa ctaagccaaa ccttctatga
    67141 tccagacaaa gaaaaagtga tggttaatta cccgaacaat tcagaaggtc gccaattaga
    67201 gcaagtgtac catgaagttt tcaaacattt attgacggtg aagaaagagc tggattatta
    67261 ttctttaccg attatcgata caggaatatt gaagtatgat gggaagaagg aacgatttat
    67321 ctttaaatct gttcgggaag atttgcctct ttcagcaggg atggatctag aggtcttggt
    67381 agaagactat tttacagaag aaaagcactg ggttcgaaca aaattggatt atttaccgca
    67441 ggcagccgga gggacacagg caagcggttg gtacatcact gaagacaaag agttagaact
    67501 agaaggtgta atggcgcgta ttcgaaaaaa aaattaaaaa agtaacatta gatatttatt
    67561 gaaggataaa ggagcgcagg aagtagcatg agagaaaaaa gagcatttta tatcaatggt
    67621 tatgcaggga ttgtcggatt ggtcgtatta gctttagttg gattgttttt cttctattta
    67681 ggtatgtggc aagcgaaagt ttgggcactt tttttaagta tttttctttg gttgatcacg
    67741 ttattgttgt taagttcggc aactgtagtc agtcctaatc aagcaaaagt aattttgttt
    67801 tttggacaat atttaggtac gattcgagaa aatggctttt tccttacgat acctttggca
    67861 caaaaaatga ctgtttcgtt gaaagttcgg aatttcaata gttctgtttt aaaagtcaat
    67921 gatttggatg gaaaccccat cgaaatctca gcagtagtag tctttaaagt cgttgataca
    67981 gcaaaggcat tattcgatgt agcgtattat caagattttg tagaaatcca aagtgagaca
    68041 gccattcggc atatcgctag ccaatacccg tatgatacat tcaatgagaa tgatttgacg
    68101 cttcgaggaa acacgacggc agtgtctgac gagttgcaaa aagagctgca agagcgttta
    68161 gcagtagcag gtgtcgaagt aatcgaaaca cgcttgaacc atttagccta tgcaacagaa
    68221 atcgctagtg ccatgctcca acgtcaacaa gcaaaagcca ttttgtctgc tcgacaaacc
    68281 attgttgaag gagctgtcac aatcactcaa atggccttag agcaaatcga agatggacaa
    68341 gaaatcaatt tcaccgatga tcggaaagtc caattgatca ataatttact tgtctcaatc
    68401 attacagaca aaggaacgca acctgttatc aatacaggcg acatgaaaga ccgtaagtga
    68461 catacttttt gtaagatagg aagaaggagt ttaggacgga ttattgtctt gaactctttt
    68521 tttagttgtt ttaaagaaaa tgacaaaatt gaaagaagaa tgatagttga ttcgaaggca
    68581 gaattgttcg cttttttcta taatcagtat ataaagtaag agagaagggg aaaaagtgaa
    68641 aaaacttctg atcggtctac ttgttttcat cgttgtggta cttgcagggg tgaaaatcgc
    68701 agattacgta gttatgggtg gagaagaata ctatgtacaa attacgacag atggtacaaa
    68761 aacagtttca aaatcagact ctggacaaga aacagtcaac tatagatata tcctgcctgg
    68821 ctatacgaaa gagggtaagg aaaaagaact ggacttcaag ggacaattga ctcgaccgct
    68881 tcgtaaagga gcatatttaa aaatcacctg gaacaaaaat aaaggtgtca cttcgtatga
    68941 agaagtcgaa aaaaaagacg tgccgaaaac agcactggaa aaactaatta aagggtaggg
    69001 aaagaaatga acaaaatagt cgaagtacag aatgtaacaa aagtatacgg aaaaaacaat
    69061 gagaaaaaga cgcaagcatt aagcggcatc agcttcgatg tagaaaaagg cgaatttatt
    69121 gggatcatgg gcgcttctgg ttctggtaag tcgacacttt tgaatatttt atccacatta
    69181 gataaaccaa cagatgggca tatccgaatc aatcaaaaag atgtgaccac attaaaagga
    69241 aatcaactag cagattttcg tgcgaatgag attggcttta ttttccaaga tttcaattta
    69301 ttagaaagct tgacagcaca agaaaatatc gctgttcctc tttctttaca aggcgtcaga
    69361 ccaaaagaaa tcaagcaacg tgtccaaaaa attgcagaac gtttatccat ctctcatatt
    69421 ttggaaagct atccttctga gatttccggt ggacaaaaac aacgggtcgc cgccgcacgt
    69481 gcattgatca cacaaccaac gattctatta ggggatgagc ctacaggtgc gctggattcc
    69541 aaaagtgccc gggatctttt agatacgatg gacgaattga atacaaaaga tcatgtatcg
    69601 attcttcttg tgacgcatga tccgttttct gcaagttatt gccaacgtat tttattcatc
    69661 aaggatggcg gtatccatca agaagtgaaa cgtggagatc aaagccgtga ttccttctat
    69721 cgagaaatac tatctattct tggaaacttg gaacagtagg gggagattga ctatgctttg
    69781 gaaattatca cttactggga tcaagagccg tttaagagac tacttggtgt tattctcagg
    69841 attagttatg gcatctgcta ttttttacat gtttgaatca atggcaagca acgaagcttt
    69901 tttgaaaagt aatacgatcg ttgcggctac agttatcatt ttccgattcg gttctgtttt
    69961 actagggatc atcacattcg tttatatttt atatgcaaat tcctttttga tgacgatgcg
    70021 gcaaaaagac tatgcaatgt ttatgatgct gggagcaaaa ggcagaaaaa tcgcacagat
    70081 gattttttct gaaacattct tagtagggat cgcagctact ttagtgggtt cagctgtcgg
    70141 tgtaggattg acaagtatcg tccaccgatt attagtggat cagttagatc tcaatatttc
    70201 gcattttaca ccttttagta ttcacgggtt gttgatcact gttttgtttt ttgctgtttt
    70261 gtttttatta gctgctgttg tcaatgcttt ttcgattgtc aaaaaaccga ttttagcatt
    70321 gattcgtgcg gatcagacac caacaagaat caagcagcat aaattcttgt ttttccttga
    70381 agtagtactg ggaattgctt ttctaggctg cggttactat atgatgaaac acgtggggac
    70441 ctatcaagtc tttggtatag gggtagcctt agttacgatc gtattaggaa cttatttcat
    70501 tttccattct gtgatcattt tctttttatc attactaaag aaatcagaac agatttcatt
    70561 gaaaaaattg aacaatttca cattgtcaca gttaaacttt cgtattcgtg aatatacaca
    70621 aatgctctca atggtcgcta tgctgtttgc cttggcactg ggcgctttga cggtaggatt
    70681 aggttttaaa aatgaaattg cagacttgac agaaaacaat tctggatatg atctagtgat
    70741 gaataatcct cgggcagaag atcagcaaaa aatcaaagaa ctttcaccaa cactttctgc
    70801 ttcttatact caaaaagaag atgcgggaac gatttactat aatgcaagtg aatttgcggc
    70861 tcaaccattg atcatgataa aacatgaaga tggaacaccg ccgaagatca cgaaagaaaa
    70921 agttcctgct aaaaaaatga atgaactgac tgtacaagag gaacttcgaa gctatgaatt
    70981 gccggaacag caagaaaaag aaatcaaggt agtcagtcaa tcagaatttg atcaactgaa
    71041 cttgccacaa tccactttac agctagttca ggtaaaagac ttccaagcaa acctgaaacc
    71101 aatcgaaaca cttgttgctc aaaataaaat gaacaatcca tctttgcaaa aagtagagta
    71161 cagcaaccag aaatatgaaa tgtatgaagt gtataatggt ctattctctg gttttgaatt
    71221 tatgggattc ttcctaggtt tagctttctt gaccatgctt gctagctgtt tgatgttcaa
    71281 gatcttgtca ggaagcaaga gtgacattgc tcgttatgaa atgcttgaaa agataggaac
    71341 acgacgcggg ttgcttaaac aatcgatccg tagagaagta ggcgtgttat tcttagcacc
    71401 aggaatctta ggtgtagtcc atgtcttatt cggattacaa atgtttggtc tattcatgca
    71461 aaatccatac aaagatattt gggttccgtt tacgatattc ttcgtattgt atttcatcta
    71521 ctatgtactg acgacatggt tatatactgg aatcgtattg aaaaagagat tgtgaaaaag
    71581 ctgtcctgca tcaagtaata agaacagcca agcaaaaata gctcctcata tttttcgttt
    71641 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga aaaagaggtt
    71701 gtgacaatat ttttgtcaca accttttttt cacggtcttt tattagaata tagtaaattt
    71761 tttctcgtta ctcacggaat aagctgttaa gaaaacggtt tccatgtata ctggaatcag
    71821 tgcaaatgaa agaaaagagg gtgcaaggaa catggaaatc aatgaacaaa attttggcga
    71881 aaactatcac ttgattacga tcacgaatca aaatgggaca tctttgtctg tcagtgatct
    71941 tggggcacgg gttgttcatt ttcaaacaat aatcgataat gaaaaacgag agttgatctt
    72001 aggttttgat gaagcaaaag aatatctaga aaaagaccct tatatcggtg cttcaatcgg
    72061 acgtatggct ggacggatta cgaatggcac attcaaatta gatgatgaaa cgttcgaagt
    72121 aaaaacagac ccggacactg gtcattgttt acatggtggt gcacctagct ttgaagccaa
    72181 gcagtggcat tatacagtca tcaatggtga aaacgaagca tcggtcattt tctatcagac
    72241 tagcccagat atggaaaacg gatttcctgg aactttagat gtagaagtac ggtatacgct
    72301 gacgaacgat gatacatggc gagtgacggt ccaagggatc agtgaccgca aaacagtatt
    72361 caatcctaca aatcatgtct atttcaattt aaccggtgat ccagcacagt caatcgacga
    72421 acatatactt tggttgaata gccgattatt tgctacgctc aataaagata caacgcctac
    72481 aggagaagta gtttcagtaa gtggcacagc atttgacttc caacgtccta aaaaattagg
    72541 tgaggtcttt gccagtgatt ttcgccaatc aatgatagtg ggcggcatcg atcatccatt
    72601 tttgttgaaa aaacaatctg gtttactaaa tgctgcggaa ttgacaagcc cagatgaaaa
    72661 agtatcgatc caagtaaaaa cagatgcttc aggaatcgtg atttttacag caaattttgg
    72721 agaagatgga ccggaaatgc gcgggaataa attagtaaat catggcggta tcacatttga
    72781 gactcaagaa ctgccaggag cggagcgact tgaaagtttt ggagatgtca ctctggaacc
    72841 taaccaagta cgcgagtttg taacggaata caaaataaat ataaagaaat agaataaatt
    72901 aaaaagggag tgtgacagtg tcacatcccc tttttaattt attctatttc tttaaaggca
    72961 gatcaagcca gaatgatttg tctaaactta acaaggttag tacaggaaca gttgcagctt
    73021 taactgtacg agtagttgtt tctggtatct gtcttgccgt aaaattgttc cacaaatccc
    73081 agattgaagc gacacggaag atggaagaag cgttgttgac gcgttttatc gttgaaaatt
    73141 ctaatcctgt gattggcacg ccatctgttg tccatttgat tggcacatcg aatcgcttag
    73201 acatattgag gatttcagcg tttgcttctt cagttgttgc tgcttctttt ttcggttcat
    73261 catattttgt caggtcgtac gcttcgatga tcgcgttcaa tttttgtgaa tattggcgat
    73321 ctgtagcata acgtccttcc aaatatttgg ttgcttcttt gtagtctttt gtttccgact
    73381 tccaagttgg tttgtagaaa tcaggattgc cagtcagtcc atttttgatc aagtcggcat
    73441 agtcttccaa actttccttg taagaaggat atttacggaa ttgtgcgcgg atttggtagc
    73501 tttgtccgga actatcttgt tcaagtgtat tgaaggtcac gctgcttcct ttatatgatc
    73561 ctttgatacc aaaaaggtta tgatttggtt cgcttgataa taagctgttt ccagatccac
    73621 tttcaaggat agcttgagcc atcatgactg atgcgtagat tccttctttt tgcccgattt
    73681 cctgtgcatc atctgcaatc tttttcaaga actgatcagt tgtttggttt tgactgaaat
    73741 ggaacgagcc atctaatcca tctaaaatag atgagccgcc tgtcagcgaa cctgaagcag
    73801 caggtaaacc tgctaagttt actcggacag caaaatcagg cgtgaaatct gaaatagaag
    73861 ttactcgaac tttatctcct ggttgaggcg catggataaa ttgtccgttc ccaatataga
    73921 tacctacatg gtgagtaaca ccggaagaag gtccccagaa gacgaggtcg ccaggttggg
    73981 cctgacttac tggaatctgc gtacctgcat attgctgttc gcctgtccag ccaccgatat
    74041 aatgaccggt tgctttcata tatacgtaat aagtcaaacc agaacagtca aaagtatttg
    74101 gaccttttgc tccccataca tatggttttc caagatgctt gaatgcttct cgaacgattg
    74161 cttgttggac attcgttgta tttgtaggca atacagcatc aattggtgat tttacaacaa
    74221 aatcagaaga agcagaagat ggattctcta taccgctttg atttgctgta tttggacgag
    74281 atgaagcatt gttcgttccg ccattcgatg aaccattgtt tggatggttt ggttctctct
    74341 gagtatttcc attgttattc gatccagatg ggtttgtttg attcgaagaa ttcccgctgt
    74401 tcgaattatt gttattattg tttgaattat ttgaaccatt attattgttg ttattaccca
    74461 catctggttt gtttggcgtt tccggatctg gtgtactagg gacttcttca ctaggatcag
    74521 ttggtttctc tgttgaagta tccggtccag ctttaaattc tgcagtaata gataaattgt
    74581 tcgtacctac tgtaaaggtc tttgcatcat aatttacttc tttggcacca ttaacagcga
    74641 ctgaagcaat ttgttcacct tcagctggcg taatagtgta agttaccttc gtttccgcta
    74701 ataaaccttc taccgttcca ctttccgtaa tatccaaagg ttgctgttca ggggaatccg
    74761 ctgtcgaagc agtcagttgg atcttaccaa tcaaagaagg agcaattgta agggaatatg
    74821 tctcggctgg cttttcattt tctgatggat cagccggatc aatttcagtt ggctcggtcg
    74881 atcctgttgt tgagttagtt gttccagttg ggtcttctgt aaaagaatcc gttgtttcac
    74941 tagtagctga gctggatgta gtcgtatctg ttgtgactgt ttgatcagct atttgattat
    75001 ctacggcaaa tacggtattg gtcgacaaaa cagacggagc aatcagccaa gtagcagcta
    75061 gcaaacgtat cgttttttta tttttcataa taaactagtc cttttccttt cattgatttt
    75121 ctgtcttatt cagcattcat tagacatttt ttggaacaga taatcgtgtc gaaaagcttt
    75181 ttcttttcat aaatctgtct gtgtattcta aataaaacaa tttgtaaata agcaacgagc
    75241 attcattcgt ttttctttga cgttcatttt attgcataga ccacctttgt ttggggataa
    75301 acaagtgggg ataaaaatgg ttatgcatat gtaaattata ccaaagtttg gaaagtatca
    75361 caaaaattta taaaaataat gatcctaaaa agaaaaagac tgaaatatgt gataaacatc
    75421 tcatacttca gtctttttca ttaactattc ataagacatt aggtcttcat caaacccgct
    75481 aacgagagta gtgatcattg ctacgactag actaacagcc agtcctgcta agtaccagaa
    75541 aaaagtaccg gaaatgaatg caggcaatgt ggtcagacta ccaaatacaa aagcgatcgc
    75601 tttcacttgg gcaaatccca ttacagctcc accagcagct gcaccaatgc aagcagcaac
    75661 aaatggtttt ttcaatggga tcgcaattcc gtaaacagtt ggttcggtca ctcccgcaag
    75721 taaaatcgaa gcaaaagcag aaccggagat cgaacgggtt tttgaacgtt tacttcgcag
    75781 aaatacacct aaagctgcac cagccattcc aaaattggct gctcctgtca acggaacgat
    75841 tcggtcgaac ccataagcag agatattact gatcatgaca gggtttacag cccattggat
    75901 accaaatatt accaaaatat tccaaccacc agcgatgatt gcgccactga tcggtccgtt
    75961 aatatccatc atccaagtaa ttgcggcagc taaaccttcg ccaaggtaaa cacccgcagg
    76021 cccgaatacc attaacgtca aaggaaccgt gataactaat gaaagtaaag gtacaaatac
    76081 taattgcata ttttcgcgaa tatatttttt gaggaatttt tcaacaaaag aataaaagaa
    76141 aatacctaaa atcgccggca tgacagtaga cgtatactgc atcagtacta caggaatccc
    76201 cagaaaatca gtagctgctt tgccactgtc tgaaagtaaa gaggtcagat tcggttcaat
    76261 cagtgctgca gcaatgacag ccgcaatata tccattcact ttaaacgttt tggcagcaga
    76321 aaaagcaaga aagattggca gaaaataaaa gacggcattc gacgcagcag acaaaatatg
    76381 ataagttccg cttgtttcac taagccagcc aatcgtagtc gctaaaacaa ccagaccttt
    76441 taatatacca gaaccggcaa acagcggaat gatcggcgta aagatttccg atatcaaacc
    76501 aaatagtttt tcgatcggat ttagcttttc tttcggagct gaatgatcga tttgacgcgt
    76561 caattccttg taagcaggag ccaatccctg atccatgatt atttctgcat atctatcgag
    76621 taaaatgaaa tacttaatgg aagggatttg ctgttttaat ttttctgcat tggctactgg
    76681 ctgttttttc aatttgatac gaagccgagt cgcacatgga aggacagaat caatattgtc
    76741 ttttcctccc aactgttcaa tcagcatatg aatctgctcg gacagtacca tctgttcagg
    76801 attcattgtc gtttccatta gaagaccact ccactcacta atataatatt ggcgtaaggt
    76861 gtcatttcgc ctgtacgaat aaatgcatga cagttattca actctttttt catttcttca
    76921 tgtgggataa agctaatcgg tgtatctggt aacaataatt tgattttttc caatatttcc
    76981 gggttcattg tctttatttc ttccgccaat tcaatcgatt gtacctcaag ttcttctaaa
    77041 acgttttcta aaacagataa aaaacttgga agaccgtttg agacagccaa atctattttt
    77101 tcagtaccaa aggggacagg catgcctgca tccccgatac ttaatttatc aaagtgcccc
    77161 atttgtgcga ttactcgtga aatatctgaa ttgattactt ttcctttttt catgtttgtt
    77221 tcttcctctc attgttaatt ttagactgct gattgttcta aagcttcgcg gtaagggata
    77281 gaaggttgcg caccataaga ctgcacagca acagaagagg ctagatttcc ataaaggatc
    77341 gcttctttca agttactaag gtctggctga agtgtgctgc ttaatgcgcc aataaatgta
    77401 tcacctgcgg ctgttgtgtc aacggcatcc actttaaaag cagggacgat accgtgttct
    77461 ttttctgtat gatagaaagc gccggcactg cctagtgtaa tgattactgt tccaatacct
    77521 aattcatgga gtttttcggc tgctgcgacc aaactgttat gatctgtcac tctgattcct
    77581 gtaatgattt ctgtttctgt ttcatttggt ataatgatat ctgtcttttc aagtagttca
    77641 gtaggaatcg tttcacgagc tggtgcagga ttcaggatcg ttgtttttcc tgcgtctttt
    77701 gcaatagaaa aagctaggat cgtcgcatct aagctacttt caaactgagc aataataaag
    77761 tcactgtttg taatcagttg cttgtttttt aggacttctt gttcatggaa agcattattt
    77821 gcccctgcat ggatcaagat actattttct cctgctgcgt ctaccataat cattgcttgt
    77881 ccggttgtgg cgccttggat ttcagctatc ccagatgtat cgatgttttc ttttgtcagt
    77941 agatctaata actgttgtcc ttcgctgtca gccccaacgc ctccaataaa agatgtccgt
    78001 gcgcctgaac gtttggctgc cactgcttgg ttggcacctt tacctccgcc agcgtgaaaa
    78061 atctcatgcg catgcatcgt ttcgcctggc ttgggcatgt tggttaaacg aagggtagtg
    78121 tccatattaa tacttccaat tactgtaacc gtgttcattt tacaaacctc caactatctc
    78181 atttattttg atgggaaaat tattttttaa aacgttttag taaaacgttt taatagttcg
    78241 ataaaaatat agcacacctt gaaagctatt tcaagggtgc agttgaaaat cttttttcta
    78301 atcgtacagg taatcttttt tcttcccatt ctttttctgg gaattggatc cgatctaaca
    78361 ataacttggc agatgtttgt cccaattcga agattggttg tgcgatcgtt gtcagtttcg
    78421 gcttgatata ctcgcacata tctatattat catagccaat aatgctgtag tcttcaggga
    78481 tgctttttcc agcttcctct aatccgcgat ataatccaaa agctaattca tcatttaaag
    78541 caaacacacc agttgcagca gaatcaagaa gttctgctgt gatctggtag ccaccttgtt
    78601 ttgagaactg tgtaggtaag aggatcagtt ggtcgtgagg gatttggtaa acatcaagag
    78661 cagatttaaa cccctcaatc cgagcgtgga cattttccgg tggattttca ggatagacaa
    78721 gggcgatcgt ttgatgtcct agactaagca agtgcatgcc cgctaggtaa ccaccgcgaa
    78781 aatcgtctgt tcgtacggca tcactaaatc cttcagattt tttctgatcc aaaacaatga
    78841 agggccggcc ttgttttttc aaattttcgt tgattgcatc tgtagagaca gcagaagtcg
    78901 cgataatgaa tccatccaca cctcgacgtg tgagctcggc tagatattcg atttcttttt
    78961 gatgatcaga atcagcatta cataatatcg tgacgaaatt ctgcttatac aagatatcct
    79021 caattcctcg catcagtgta ctgaagaatg gattcgtgat atcaggaacg agtacaccaa
    79081 ttgttttcgt ttccttggta atcatttgtc tagcaaaata gtcaggctga tagcctaatt
    79141 cttctttggc agctaaaact ttctgaacag ttttcggact gaatttctcc gtatttccat
    79201 ttaatatcaa ggagacagtt gtgattgaaa cgccggaaag tttagcgact tctttgattg
    79261 tgatcttttt tttcaccatg ttcatactcc tatctaaatc ctgtggttat tttcccatga
    79321 aatcaaaaag aagtaaatga aaatcacgtg tgccagtact tatttgcttt gttggatagc
    79381 ttcgattatt tgtttcgttg agcgtactcg tgcagttttc ggaaatatct tttttatgaa
    79441 caattgatgg gattctttgt cacgatcact gcaagcatct tcagcaagca cgatatggta
    79501 cccgtgttga aaggcatcta atgcagtagc atatacaccg tttgttgtcg ctacaccgct
    79561 taaaatgata gtttcgattc cacgccggcg aagttgaaga tcaagagaag taccaaaaaa
    79621 agcacttgga ttgtattttg tgatagtcaa catattatct gtatctttca aagaatcctt
    79681 caaaagtaaa tcagtgaatt ctggaggtac agaagcagtc attccaccca tatcagtttg
    79741 ttgactcaaa taagggaaag tggagatgtc tacgttcact aaaactttca aggcagcggt
    79801 acgtttcaat gttcttacta acaaatcatt gtttgtcaga atagatgctg cagtgtgtgg
    79861 agagagtgta ccgttattta cgatctcttt ttgcatgtcg atcgcgacaa aagcagtttt
    79921 tgataaatca aaaatcatat tttcctttaa atcattcatg atgttccttc tttcaaataa
    79981 caatgttttt tatgtaagta aatcataaat cgtttatttg tcaactaaat taaaggattc
    80041 ctttctttag tctagagaaa ttcagtggta aaataaataa gtgtgagaaa acgcattcga
    80101 tgcatagata tagcgagaaa tatcgtgaaa atggaggaat cagagtgaag tatgtcgaag
    80161 taaaaaatga atacaaacga tacaaaatgg gagaaacgac cattacagca aatgacgata
    80221 tttctttttc aattgaaaaa ggcgaattag tgattatact tggtccaagt ggagcaggaa
    80281 agtcaacgat tcttaatatt ttaggtggga tggatacacc tgatgaaggt cagatcatta
    80341 ttgatgacac agatattgcg caattttcag ataaacagct tactgcttac cgacgaacag
    80401 atgtagggtt cgttttccaa ttctacaatc tcgttcctaa tctaacagca aaagaaaatg
    80461 tggaattggc aacggaagtt tctcctgatg cgcttgatcc tgtagaggta ttgaggcaag
    80521 tgggcctagc tcatcgattg aataactttc cgtcccaatt atcaggaggc gaacagcaga
    80581 gggtttcaat tgctcgtgca ctagctaaaa atcccaaact tcttttatgt gatgaaccga
    80641 caggcgcgct ggattttgaa acgggcaagc aagtattgaa attattgcaa aacgctagca
    80701 gacaacacgg caataccgtt ttgatcatta cacataattc tgctttagcg cccatcgctg
    80761 atagagtcat tcacattaat gatgctaaag taaggtctgt tgaattaaat gatcatcctt
    80821 catccattga tgagattgta tggtaggggg attttatgaa aacaagaaca tatcttaaag
    80881 caggttttcg cgaaattcgg caatctaaag gacgattcat tgcaatcatc cttattgtca
    80941 tgctaggaac acttttattt gtaggtgtca aaactgctgg acctgtcatg caaaaaacga
    81001 tggatcacta tgttggtaca gcaggacttt ctgatttgca aatcgtttct actggtggct
    81061 tgacacaaaa agatattgct caagcagaaa agatcccaga tgcaaaagta gaaacaggaa
    81121 aacagatcta ttattccaat actggcaaaa acgaagtgat tcaaatattt tcttataaca
    81181 aaaataacaa gcagaacaag ctacaattaa ctgatggaaa actcccggaa aaaccaaacc
    81241 agcttgtatt ggatgaaaaa gcggaagatg aagggtataa aatcggtgat atttatcaga
    81301 ttgattcaga tgaattgaaa gaaaaagagt ataccatcac gggctttgtc cgttctccat
    81361 tgttcatcaa caatttggag agaggatacg cgaacgtcgg aaatggtagc gtcgattatt
    81421 ttgtctattt acctgaatcc gtttttaaaa cagatattta ttctgtctta tatcttgatt
    81481 ttacaaacgt aaagaatttg aatacgtaca gcaaagctta caaagataaa atggagcaaa
    81541 atcaagaaaa ggcagaaaag tatttacaag atcgacccga agaacgctta gaagagctga
    81601 aaaaatcggc caacgaagaa ttagaaccgg cacagcaaaa aatagcggat ggcaaagcac
    81661 aaacaacgca agcaagaaca cggttagaag atgctaaaaa acagttagcg caacagtcag
    81721 caactatcca gcagcttcct gaagcgcaac gaacaatcgc tgttcaaaca ttatctcaac
    81781 aagaagctca acttgctgaa caagaaaggc aattagttga gaaagaacgt gaactggcct
    81841 ctgcagagga agaattgaat cgaaatgcag aaaaaattga aaatttaact aaaccatcat
    81901 acctatacaa tgaacgttca gaaaatgtcg gttttcaaga atttggtgat ctgtctgatc
    81961 gtatcgctgc gattgccaat gtttttcctg ttttcttttt ctttattgcc gcattgatca
    82021 cctttacgac aatgacacgt atggtagaag aaaaccgcag ggaaatcggg acattaaagg
    82081 cgttaggtta tacgaaagtt gaaatcgcaa aaaaatatgc aatctatgca agtctagcct
    82141 cagggatagg tattgtttta ggaacgatat taggaacgaa tcttttgcca aggatcattt
    82201 ttgagctgtc taacgagcgg tatgatattg ggagtgcgct tattttttat gactggccgc
    82261 cgattttaca agcagctgcc gcattcttca ttgccgcatt tggcgctgca atgatcgttt
    82321 tattcaaaga tttaagagaa cggcctgctg cattgcttca accgaaagca ccgaagccag
    82381 ggaaacgcat ttttcttgaa tacatcactc cgctgtggtc acgccttagc tttaatcaaa
    82441 aaatcagtta ccgaaatctc ttccgatata agtcaagaat gcttatggcg attatcggaa
    82501 ttgctggatg tgccgggctg atggttgcag gcgtgggatt gaaagattct ctaagttctg
    82561 tttcagccaa acaatttgga ccgatcattg actatcaagc aatcgttaca ttggattcat
    82621 ccacggaaga tagtcgtcaa aaagcagcgg atatgctgca gaagcaagaa aaagtaaatg
    82681 agatcctagc tattcatact caaaccgtag aattgagaaa aaaaggacat gcttcgcaaa
    82741 gtctgacagt gatcgtacca agtaaaacag ctgatttgga tcgttttatc catttaactg
    82801 atcttgaaga gaacccacgc tccttgccta aaacaggaat cgctattacg caaaaagtag
    82861 cagaactatt tgacttatct acaggagatc atatttcgct ttatgatgaa gatcaaaaag
    82921 aatggaaagt cgaagtaaaa gaaatcgtga aaaactatct aggtagcttt atttatatgt
    82981 cccctgaata ttatgcttca gttactgatc aatcgatgat ggataatgca tttttgctgc
    83041 gtacagataa gatgacaaca aaagaagaag aaatgctttc cgagaaactg cttgccacag
    83101 acgctgtgac aaatacgtct ttcatgtcca aacaaatcga aacgcaagaa gaatcaatgg
    83161 cgaacttaga ctcggttgtc ttaatctttg tcgtattatc aggactgctg gcttttatcg
    83221 tcttgtataa tctaactaat atcaatattt ccgagcgtgt acgcgaatta tctacaataa
    83281 aagtccttgg attttttgat aaagaagtca cgatgtacat tgttcgagaa aatatcatct
    83341 ttactctttt aggaatcatc ggaggatttg gtgtaggata tatattgact gacttcatct
    83401 tgcaacaagc gtctatggag aatgtcatct ttccattagt gatcacatgg tgggcttatg
    83461 tcttatcagc aggattaact atcgtattta cggttattgt gatgattgtt acccatttca
    83521 aattaaagca tatcgatatg attgatgcgt tgaaatcgaa tgaataaaaa cagaggttgt
    83581 gaaatcagcg gtttgacctg agtattagag aaacaaacgg caaaatagtt tttcatattt
    83641 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccatgaat cagaggttgt
    83701 aaaaaagccg tcctgcatcg agcaaaaaga atactcaaat aaaattagct cctcatgttt
    83761 tacatttgag aagactgatt gccgtagata aaagcttttg aacaccattt atagaagaaa
    83821 caagaatatt ttgctatttt aaacaagagg atagacaaat acatgtctga tctcttgttt
    83881 tttattgggc aacagacctc ttagaatgta taaagataaa ttttacaaac gctttcggtt
    83941 cagttataat aataaatgat aacttgtata tataagttgt ggtaggtaat aaatcagttg
    84001 aatggaggta aatatccatg acatttaaag acaaagtcat ttaccaaatc tatcccaaat
    84061 cttacaaaga cagtaatggt gatggaattg gagatttaaa aggaatcaaa gaaaaactcc
    84121 cgtatctgaa tgatctaggc atcgacatga tttggctaaa ccctatttat ccaagccctc
    84181 aaaaagacaa cggttatgat atctctgatt acacagccat cgatcctatc tttggtacga
    84241 tggaggaatt cgaacagcta atagccgaag caaagaaata ccggatagaa atcatgctcg
    84301 atatggtgct gaatcacgtt tcgacagaac atgaatggtt caaaaaagca ctagcagggg
    84361 accagcatta tcaggacttc ttcattttaa gagaagagcc aacggattgg gtatcaaaat
    84421 ttggtggaag tgcttggtcg ccatttggag atacggggaa atattatttg cacttatatg
    84481 atcggacaca agctgacttg aactggcgga accaagcagt aagagaggaa ctattcaaag
    84541 ttgttcgttt ttggatggaa aaaggggtaa aaggattccg tttcgatgtt atcaatgtta
    84601 tcggtaaaga cgaacaattg aaaaataata aagaaaatga tggcaaacct gaatatacgg
    84661 acaaaccaat cacccatacg tatctaaaag aattgaacca agcaacgttt gggacggatt
    84721 ccactatcat cacagtcggt gaaatgagct ctacgacgat cgaaaattgt atcttgtata
    84781 cagaacctag tagagaagaa ttatcaatgg tctttaattt ccatcatttg aaagtcgatt
    84841 atgaaaatgg gaataaatgg tctactcctc catttgattt tgcagaatta aagcgactat
    84901 ttcatacatg gggagaaaag atgagtgagg gtaacggctg gaatgctcta ttttttaata
    84961 atcatgatca gccacgtgcg ctcaatcgat ttgtagctat cgatcagtat cgaaaacaag
    85021 gagcagagat gctagcaacc atgatccatt tgaaccgtgg gacgccctat atttatatgg
    85081 gagaagaaat cggcatgatc gacccagact attcatcgat ttccgactat attgacgtag
    85141 aaagtttgaa tgcttataag ttactccttg aacaaggatt cacagaaaaa gaagcgtttc
    85201 gtcggatcaa agcaaaatct cgcgataact ctcgaacacc catgcaatgg tcagacacag
    85261 aacaagcagg atttactaca gggaaacctt ggttaaaagt tggaggcaag ctagaagaga
    85321 ttaatgtaga aaaagaacta tcttctgaag attctatttt gtcctattac aagaaattga
    85381 ttcggctgag aaaaacatat ccaatcgttg cacaaggaga ttatcatgct tatgaagcaa
    85441 atcatccgga agtttatggc tttcttcgcc aatttgaagg acagcagctg cttgtattaa
    85501 caaatttcta tgataaagat acaaggatcg ccatcccaga aggattcaca gaagccaata
    85561 ttcttatcga taattattcc gatactgttc ttcatcaaag tctggagcta aaaccatacc
    85621 aggcaattgc tttgctgaaa aattaaaaca aaaacacaaa acgtcgttcc tacgagctat
    85681 tctcgtaaga tcgacgtttt gtgttttatt taaaataatc ttcttcagaa aatgaagagt
    85741 tttcatactc tcctttatcg ccataaaatt tattgtagcg ttgtttgaat ttacggaaaa
    85801 taaaagtaac gagtaatttg actaacgtat agataggaat accaaaaata acgcccacaa
    85861 ttcccattag atcacccatg acaagtaaaa cgattaagat cgtaatcgga tggatctgta
    85921 aacgatcacc cattactcga ggaatcacca agtcaccgtg gagaagttgt acggcaaacc
    85981 agacaatgac aaatttgaca gccataaaag tagagtcttg gaatgcaatg atcaaacctg
    86041 gaaagaaggc gataaacggc ccaatatatg gaatgataca taacaaccca gcagaaatag
    86101 ctaaaacact agcaaattct aatccgatca gtaagaaaca aacccagtag actgctccaa
    86161 ggataaatga agcaatgatt tgccccttta gaaatgaacc caattgttga ttagcaatct
    86221 ttgtcaaatt tttaaaatct gtacgaaatg ctggtgggac aatcgctaaa actgactgat
    86281 aaaatttttg aggatctctc aaaaggaaaa aagcgacaat tggaccagta aataaagtaa
    86341 taaaaatagc tgtaacagtt gaaaaaatgt tgctgatccg ttgtgcgcca ctttcccaat
    86401 aatttccaat aaatccagct atatcagacg tcacgtcgtc aatagaagcg aaaaattgat
    86461 taaatgaatc ggcaaaaggt gttttctcca aaaattgtcg gagactctct tgaaaatctt
    86521 gaaacaaatc tggcaaacta ctgatcaatg tctttgcctg ttcttcaaga aaaggaataa
    86581 gccatatgct tccaagcccc aataaaaata aaataatcac ataaacgata atgactgaca
    86641 agttcctcga aaggtgcttg ctcaaacgtt tgatgactgg ttccaaaaga taatacaaga
    86701 tcagtgcaaa caataatggg ggcaagatag tagcgataat gattccgaca ggcttgaata
    86761 taaacgagat ctgatggaaa aaataaatga tcagcgcaag taagataact attcctaaaa
    86821 catagtaaga ggtttttcct cctaaaaagc ggatcaaacg attcgttgta tctgtttctt
    86881 ttttcactct ttttcctcct ttatctataa cttcattata gacaaaagcg ggtataaaag
    86941 gaaaatgata gaggttgatt gcaaaaatat cgtcaatcat tcatataaac tttctcatca
    87001 attaaaataa gaaaacgctt gacagttctt ttaaccatat gataaattaa atcctgttgc
    87061 taaaacgtaa ctattacggt ttgcgaaaag cgaatcggaa cattcaaatg aaagaagatg
    87121 agaagaggag tgtcataaat gaaacgattg ataggtatat tggccatgct ggttttagca
    87181 ggtatgttaa ctgcttgcgg ggcaagtggg aaagccgagg acagtaaaga aaaattgagc
    87241 gtgatgacga ccttttatcc catgtatgat tttacaaaag caatcgtcgg ggatgaagga
    87301 gaagtagagt tattgattcc agcagggact gattcccacg attatgaacc ttctgctaaa
    87361 gacatggcaa agatccaaga tacagatatc tttgtttata atgatgaaaa catggaaaca
    87421 tgggtccccg caatccaaaa aatattacaa gaaggcaatg tccatacgat aaaagcgaca
    87481 gaaggtatgc ttttacttcc gggaagtgaa gaaggacatg atcatgacca tgaacacggg
    87541 gaagaaggcc atacacatga gctagaccca catgtatggc tagctccaag tttagcaatc
    87601 aagcaggtag caaacatacg tgatcagctg attgaagctt acccagaaaa acaggaagta
    87661 tggacaaaaa atgcagcagc ctataccgag aaacttcaag cactccatca gctttatcaa
    87721 gaaaccttta agcaagcaaa acaaagatct tttgtcacgc aacatgctgc attcaactat
    87781 ctagcgttag aatatggatt gaatcaagta tcgattgctg gtctttcttc tagtgaagaa
    87841 ccgtctgcgg cgagaattgc tgaactgaaa cactttgtta aagaacatgg aatcaactat
    87901 atttattttg aagaaaacgc caaagatagt attgccagaa cgttagcaaa cgaagcaggt
    87961 gtatctttag aagtattgaa tcctttggaa ggattgacga atgaacaaat agaaaatgga
    88021 gaaaactatc tttctatcat ggaagcaaac ctcgaagcgt tgaaaaaaac gacagagaca
    88081 gaaaacccgc tagaagatac tttgaccccc aaaaaagaaa agagcgtcta caacggttat
    88141 tttgaagacg aggatgtaca agatcgctct ctttcagatt gggcaggaac ttggcaaaca
    88201 gtagatacgt atgtagaaga tgggactttt gatcctgttt ttgaatataa agaaaaattg
    88261 aatcaggata agacagcaca agaatataaa gaatattata cgaagggcta tcaaacagat
    88321 attgcttcaa tcaagatcag tgagaaagag atggcattta cgtttaagaa tggaacaacg
    88381 aaacgttcaa attatcgtta tgtagggaaa gaaatcttga cctacgaggc aggaaaccgt
    88441 ggcgtaaggt atctattcga agcagaagat aaggaaagtg gcgcttttag atatattcaa
    88501 ttcagtgatc acacgattgc accggctgac tccgaacatt ttcatttata tgcaggaaat
    88561 gaaagtcaag aagtattgct gaaagaaatg gagaattggc caacatttta tccagataca
    88621 atggatggac gtgaaattgc acaggaaatg cttttgcact gataggcaaa aaagaaccga
    88681 agggtttgga tcggctggtc atttagtcgg tccaaactat tcggttcttt ttatgaaatc
    88741 aatttcttta atccacggat agaggctggg atggtctcgc cgtttgccaa cgtgacttct
    88801 ctggtttttt tattaatcga ccgcacattt tgtgtattga taacatagga tttatgacaa
    88861 cggataaaat tccgatgcat tttctcgatt tctttgatct tctcatagaa ttgtaattgg
    88921 gcatgagtca aatggacttc cagtttatgt ggcgctccta gcgaagtaga aaaatattgg
    88981 atgtctttga tcggtaaaaa tttgatctca tcattaaact ctattttgaa gatatctttt
    89041 tggatttggg acatttgaaa atcttcgaaa attttgctca ggatatgctc gacattttct
    89101 tttaaatcat aaagatcttc tttgacgata taatcagtcg gttcgatgtt gctttttaag
    89161 attttcatcg acatttccgt atgggaagtc acatagatta tttttcctaa aggatctaat
    89221 tcacggatca atttaccgat cgctacacca tccatttcac tgtcttctag ttcgatatct
    89281 aaaaagtaaa taccaaaaga agaagttctg atcttggctg cattgagaag ttcttccgga
    89341 tcagaggtag aaatatggac ataactgtct aagttttcaa tcatgattct atttttgatc
    89401 accgttgata aaacttctaa ttgttttgga tcgtcttcac aaatataaat gggaatcata
    89461 tcattactcc tttattttat ttgtatataa atcgtttgag aaaaacgctg gttttcaatt
    89521 gatgtttcaa ggaaaagata aggattggtc tttgtcaatt catccaagat aaacaagcca
    89581 aggccttcgt tttttggatt tgtttttgtt gagaaccctt tttgtttcaa ctggtttaac
    89641 ggtaatgtat ttgttgttgt attgatcaca cgaatcatca cggaatgatt cttcttgagt
    89701 atggtcaatt cgattttttt cttctttgca gcactagctc cttcaatcgc attgtccaag
    89761 ataataccaa gcatacgtac aaggtttacc gtatgtttcg tatccagttc gattggctca
    89821 ggaaaatgaa gctggacgat caaccctttt tcctgtgcca tcatgagttt taatgacagt
    89881 aaactacgga tctcaggaac tttcatatta tccaacatac ttagattggc cggaaaaagg
    89941 ttcatcatct ggcgtgtagg taatatcgtt tgatcataat aagaccgtaa tccttctaag
    90001 tcgccttctg aaatatagct gtgaagagaa aaaagcagat tttggtaatc atgtttgaat
    90061 tctctcaagt cttgatagtt tttttctaga tctttgatat aatcctgttg catcctgact
    90121 tgttgctcta ataactgatc agctaatttc ttattagaga ttttccaata ataaaccaag
    90181 atgatcaggc caacacacag atatagaatg aaaaaactcg tattcaaagc taagacatca
    90241 ggtgtttcac cagaaaaacg ggtataaaaa ataatgatat agtatgtaaa taatatcaat
    90301 gtcccgatcg taccgatcat acggcggtcc acagtgtgaa accaagtctc taacgatttt
    90361 tttaaacaaa aagctaataa agcagataaa aaagcggata agacaatgtg aaaatactgc
    90421 aatgaatctc ctggttcaat aaccgaagat ttcaaaacaa aaaaatcgcc gatgcttgtt
    90481 aagtgatctc ctaaaagcga cgtcaataac ccaaaaggaa gcgcgtaaac caattgttga
    90541 cgcgaagtcg aaaagaagaa aaaagtaagc atcagcattc ctatagcaat tagagcagta
    90601 aaaatcccta aaatcgaata gacccatcca atcaaaatac aagaaatcag aatgactccc
    90661 gcaaacagat atttgtatcg gatgtgatgg tcaatcaata agatacaaat tgtgtaaatc
    90721 aaatattgca acgctatgga ccataaaaac ataaagtaca gctcccattg atttttacca
    90781 aattatagca caattggcgt attatttttt taatttttca gggattttga attcagaact
    90841 cataccaaag cagcagccag atacaaaaaa gtcagataag ttagttaatt gtttaatgat
    90901 tgtttttttc atgaaagatt cctccaaaat tcatcaagta gtttgtaata ggaagcaacg
    90961 tagctatttc taaaacaatc ccaagtagca aataggtaga aaagtaagaa gctgagaaaa
    91021 ataacgtaat gagagaaaaa attagaaaca tgctgatcgc cttctttaat aatgggggac
    91081 gtttctcggg ttcaatggga ttgattgctg tacctcttga tccatagcga agcagtaaca
    91141 aaaagcagct gattaacaaa aagaccatca ttgggaagga gatagtcaat tgcagatgtt
    91201 tcatcaccaa gggaacgccc cataaataaa gtagtgtcca cagcagacaa acgaattcag
    91261 agtcggaatg caatccataa gcatatctgc ggatgcagat ataagaaaga aaggtgattc
    91321 ctgtttccgc ccatgagcct gtcaccagag aaaagactag aacgaccgct agttttccaa
    91381 gattattcaa taaaacttct aagacaaatt ttacttggac atacaccata tcttcttccg
    91441 atttgtctcc gctgaataag gcttctaata atttgttcac aactttatct ttcatctcat
    91501 acgtacctcc accacaaatt ttatgctaaa aaaaataaaa aacgtctttt gtttcataaa
    91561 aagtggtttt gacatcaaaa ccgtcatgat ctcctaaaaa aatcttcgtt ttccaagaac
    91621 cttaccaaaa aaagtgacag ttttgatacg aaaagtactt tttatgacaa gtttgtgctt
    91681 tttcgagtga tttatgttaa aaaattactg accttataaa aggtgatgga gggattctta
    91741 tggaaaatga acaattgttt gaagtcggcg aaacatatgc atttaaatct gatttatttg
    91801 aatcaccaat aaaaggaaaa atagaaaaaa tctatgaaaa tagtgtgatg atacgtgtca
    91861 tgaaatgcgc agtagaagac aatgataccg ctcataatct gcactggcag ttagttgtac
    91921 gcaaatcaag tacaatagaa gaataaaaac agataaaatg ttgagagcat aaagaggttg
    91981 tgagtctccc gtttagctct gagttacaag gaataatgtt aaaaaacagc ttttcatgtt
    92041 ttatgaatat tgccttgtga ccgaggtgtt ggcaggagaa tactgtttat ttgaaaaaag
    92101 ggctgcgaca agacttttgt cacagccttt attttcatga ataggagtta cttttggcta
    92161 cttttagaga agtagctttt aaggagcaga taaagtcaag gcttttattt ctataaatga
    92221 agtagtaaaa tgaagaacag attttattac aggaaggatg ccgataattg tgatcattgt
    92281 tgcaggaacg atcggtgcag ggaaaagcac actaactgaa atgcttgccc aagatttaga
    92341 aacaaaacct ttttatgaga acgtggaaga taatgaagta ttgccgctat tttattctaa
    92401 tccagaaaag tatacttttt tattacagat tttctttttg aacaaacgct ttttggcaat
    92461 caaagatgct ttcagccatg atgacaacgt actagaccgc tccatctacg aagatagtat
    92521 gctgtttcat ttgaatgcag atttagggcg tgtttctgaa gtagaagtaa agcagtatga
    92581 aggtctttta gaaacgatgc tgaaggagtt agaagaaatt tcgccacaaa agaaaccaga
    92641 tctactggtt tatattcgtg tatcctttga aactatgtta gcgagaatca aaaaaagagg
    92701 ccgggaatat gaacagctag aacaagatcc tgaactctat tcctactata aagaactcaa
    92761 tcgacgatac gaagagtggt atgaacagtt tgatatatgt cccaaaatag tgatcgatgg
    92821 ggacaaatat gatttcgtag ccgatccggc atgtggagaa caaatcgtac aagaaatcaa
    92881 gatgcgggca aagaaaatga cagaagaggc agatgcccag tcgatccgct acactacaaa
    92941 taacccgaaa gcaaagggaa tctttacaac taaataatat aaaaactaag caaattgctc
    93001 actcgcagat atttctctgt gagcagcatc ttgcttagtt tttttgattc attgagatca
    93061 aatagtgaac cgattcgtat gataaaattg ccataaatga aatgtctgag tgagggccat
    93121 gatcaaaaat aaaatggaaa cgatcaaaat cgaatgggcg ccaaacagat gatgacagaa
    93181 aacaaggaca atagctcctg cgataaaggc aagaacgaca cttagatagt ggaaaaatcg
    93241 atcaaaatct gctttttctt tagaaataca ataatcatat aagttcgttg ctaccccttt
    93301 taaatttcca gttgtaaata aattagtgta ggcacgcccg ttgattttat caaaagctac
    93361 ccactggatc gatgcagtaa aagcaatcaa aatcgttact aatgcgggat gctcatggac
    93421 gggtgtcaaa ctaacaaaga aaaaaatcac tgcttcataa tatagaacaa acaagcgcca
    93481 gaaatgtttt tctttttttc taaaataatc aattagatac tttgttagaa gaatccctaa
    93541 gaaaaagaaa agggtagaca atactttttt accaacactt agccattctc cgtcaaatgc
    93601 ttggatgatc gatagaatga tattgccagt ttgagcagag gcaaaagcac catattggat
    93661 ataagtataa ctgtccatgg ctcctccgat aaatgtcagc aaaagtccta ttgtacgccc
    93721 ctcgtgaaag gaagtctgca tttttttcat caccttttat gaatattttt gtcttcctta
    93781 tcttagcata aacgattgaa aaaaaggaaa aattttcaga aaactaaaat catatcgctt
    93841 cccattttca tttttagaaa ggtctgttag tataaagaaa aaatagacaa cgaaacttta
    93901 aataaccaac ttgaaataaa caaattcaaa gaagaaagat gggaaggaaa tcaatcagtt
    93961 gaatgaaaaa gaaattctcc gattacaaaa agaattgaaa cgctgggaga acaagtataa
    94021 aaagcttcaa caagtctttc ggctacaaga agaaatcatt gctacctatg aagcggctta
    94081 tggcgaagca cccggactta ttgaggaaga agtacaagta aaagaaagtg aaggaaacag
    94141 ctctttcttt aaaatgatgg agaaacgttc ctataatcgc ttcaatcaag aacaaaaagc
    94201 agctatcaca tatgatatga agaaacatct tcgaatcata gcaggagctg ggagcggcaa
    94261 aacgcaaaca atctgtgcaa aagctgttta tctgatgacg caaaaacaag tagacgaaga
    94321 acgcatacta atgatcactt ttacaaggaa cgctgcaaat gaactaaaaa aacgagtaga
    94381 taatttcagc cagagaaaaa ccgctgtcca tatcggaaca ttccatagta tcttttttag
    94441 gatatacaat gaaatttgtc gaaaatttcc tgaagtagca atgcaaggaa tacaaggaga
    94501 cttttcaaag gatactgcac aaaaagtgaa tgctgttttg caacagctga tacgtaaata
    94561 taatctttat atatttgatc aatatggaga aaaaacgatt gcttctcggt tagactattg
    94621 gcagaatatg aacctttcag tagaagaaat ggttcagtta gttaaagaaa aatatgattc
    94681 cattgataag aatgcacgac agccgatatc agaacgactg tatggattgc tcacggaact
    94741 ccaagaacaa aaaagaagcc agcaattgct agagttcaat gatgtactcc aaaatctgaa
    94801 aagcgctttg gagaatgagg agatccagca ttatatcggc caaaaatatg attatctatt
    94861 cattgatgaa tttcaagata ctaatccact tcaatgggaa atcgtgaaac ggatgacaaa
    94921 ggaaaacagg atcaagttga ttgttgttgg agatgatgat cagagtatct atggattcag
    94981 aggatcagag ccagcataca tcaagaactt tgaaaaagaa tatcctacca aaactctttt
    95041 cttattaact aattatcgtt ctcgggcagc tattgtccaa ggagcaaata gattgatcag
    95101 ctataataaa aatgatcgga taccaaaggc gatgattcca gcacaaaaag aagcaggtgt
    95161 tatggaagcc tatttgtttt ctgatactaa agcagaaagt cagtggctaa tcaaacaaat
    95221 tcagacattt attcaaaaaa atgggaaata taaggaatca atcatattat accgatcagc
    95281 ttcacaaacg cagcagcttg tccaatctct gctgaaaacg aatattcctt ttgtactgga
    95341 agcagatagt ccatatgaag gtatctttgg tatcaagtct ttccaacatt tctatcaaaa
    95401 aattgttcat tggcaaacag cactgaatct gcaaaagaaa aatcaggcct accagcaatt
    95461 gctccggcag ctgatggcag actgctacct gaaaaaaagt gaatgcgatc aatacttttc
    95521 tcctaaatat caaaatatag ccgttgcaga ttatattttg tcgataagcc ccaatttaaa
    95581 atcgaaatct gcagaaatcc gagagtttga ggaagcgttg cgtaaaacag accagcatca
    95641 agaaatctat tcgcttgttc aaagctatct tcatctgcca cgtatagcta aggaaatcga
    95701 ccagagtgat caagaatgga tacgagaaga agtaaagcag catgctactt tcagaagact
    95761 ccaagcctta tcgcaagaaa cccaagcgac agctaaagaa ttaaaaaccc gcttgattgc
    95821 ttacagacaa ggaaaacttg atgcactatg tatccagagc attcacaaaa gtaaaggtct
    95881 tagttaccga aatgtctttt tgattggttg taacgaagga agtttgcctt ataacggagc
    95941 gacagaaaag aaagtaatcg actttaatga aataaaagca gaaccagtaa cgacgataga
    96001 agaagaacga agactatttt atgttgcgat gactagagca agaaatcgtc tgtatctatg
    96061 tgtacctcaa atgaaaaatt caagaaaatt gaaagtatcg agatttatca aagaaaccgg
    96121 gagtcgtgtg aaaaaaggaa agtgatagaa gagagggtgt gacagaagtg gtcagtttca
    96181 agaaagaaga aggaatgtac gaaaattgct tctcaaattt ttgtgaaatt tcagcttatt
    96241 ttcgaagaag ctatttctgg aacacagttt attcggaaaa gggaggtgtg acaagaattt
    96301 tgtcacacct cccttattta ttttgaaaat gagaatacta ttatcaaaat agagaatata
    96361 tagaaaaatt ctcattattc aaacgtaaat aatcagttat tttgatttt
//
LOCUS       DASHFF010000004        90125 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.004, whole genome shotgun sequence.
ACCESSION   DASHFF010000004 DASHFF010000000
VERSION     DASHFF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.004"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="U2I75_000290"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="U2I75_000290"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN1695454.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="U2I75_000291"
     CDS             1011..2258
                     /locus_tag="U2I75_000291"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN1695455.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="U2I75_000292"
     CDS             2334..2678
                     /locus_tag="U2I75_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695456.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="U2I75_000293"
     CDS             complement(2795..3121)
                     /locus_tag="U2I75_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695457.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4695)
                     /locus_tag="U2I75_000294"
                     /pseudo
     CDS             complement(3494..4695)
                     /locus_tag="U2I75_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4812..5327
                     /locus_tag="U2I75_000295"
     CDS             4812..5327
                     /locus_tag="U2I75_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1695458.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5526..5972)
                     /locus_tag="U2I75_000296"
     CDS             complement(5526..5972)
                     /locus_tag="U2I75_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1695459.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6019..7497)
                     /gene="msr(C)"
                     /locus_tag="U2I75_000297"
     CDS             complement(6019..7497)
                     /gene="msr(C)"
                     /locus_tag="U2I75_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1695460.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7851..8480)
                     /locus_tag="U2I75_000298"
     CDS             complement(7851..8480)
                     /locus_tag="U2I75_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1695461.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8464..8928)
                     /locus_tag="U2I75_000299"
     CDS             complement(8464..8928)
                     /locus_tag="U2I75_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1695462.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9415..10683)
                     /locus_tag="U2I75_000300"
     CDS             complement(9415..10683)
                     /locus_tag="U2I75_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1695463.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10914..11651)
                     /locus_tag="U2I75_000301"
     CDS             complement(10914..11651)
                     /locus_tag="U2I75_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1695464.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11718..12287)
                     /locus_tag="U2I75_000302"
     CDS             complement(11718..12287)
                     /locus_tag="U2I75_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN1695465.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12415..13287
                     /locus_tag="U2I75_000303"
     CDS             12415..13287
                     /locus_tag="U2I75_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1695466.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13629..14501
                     /locus_tag="U2I75_000304"
     CDS             13629..14501
                     /locus_tag="U2I75_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1695467.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14498..16456
                     /locus_tag="U2I75_000305"
     CDS             14498..16456
                     /locus_tag="U2I75_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1695468.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16707..17558)
                     /locus_tag="U2I75_000306"
     CDS             complement(16707..17558)
                     /locus_tag="U2I75_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1695469.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17681..18148
                     /locus_tag="U2I75_000307"
     CDS             17681..18148
                     /locus_tag="U2I75_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN1695470.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18411..18893)
                     /locus_tag="U2I75_000308"
     CDS             complement(18411..18893)
                     /locus_tag="U2I75_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN1695471.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18993..19856)
                     /locus_tag="U2I75_000309"
     CDS             complement(18993..19856)
                     /locus_tag="U2I75_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1695472.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20454..21302
                     /locus_tag="U2I75_000310"
     CDS             20454..21302
                     /locus_tag="U2I75_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695473.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21473..21814)
                     /locus_tag="U2I75_000311"
     CDS             complement(21473..21814)
                     /locus_tag="U2I75_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HEN1695474.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21900..22607
                     /locus_tag="U2I75_000312"
     CDS             21900..22607
                     /locus_tag="U2I75_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1695475.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22594..22917
                     /locus_tag="U2I75_000313"
     CDS             22594..22917
                     /locus_tag="U2I75_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1695476.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23006..24043)
                     /locus_tag="U2I75_000314"
     CDS             complement(23006..24043)
                     /locus_tag="U2I75_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1695477.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24490..25473)
                     /locus_tag="U2I75_000315"
     CDS             complement(24490..25473)
                     /locus_tag="U2I75_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN1695478.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25889..26659)
                     /locus_tag="U2I75_000316"
     CDS             complement(25889..26659)
                     /locus_tag="U2I75_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695479.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26652..27587)
                     /locus_tag="U2I75_000317"
     CDS             complement(26652..27587)
                     /locus_tag="U2I75_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695480.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28181..29830)
                     /locus_tag="U2I75_000318"
     CDS             complement(28181..29830)
                     /locus_tag="U2I75_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1695481.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30025..30921)
                     /locus_tag="U2I75_000319"
     CDS             complement(30025..30921)
                     /locus_tag="U2I75_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1695482.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30914..31594)
                     /locus_tag="U2I75_000320"
     CDS             complement(30914..31594)
                     /locus_tag="U2I75_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1695483.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31595..32377)
                     /locus_tag="U2I75_000321"
     CDS             complement(31595..32377)
                     /locus_tag="U2I75_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695484.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32370..33287)
                     /locus_tag="U2I75_000322"
     CDS             complement(32370..33287)
                     /locus_tag="U2I75_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1695485.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33290..33574)
                     /locus_tag="U2I75_000323"
     CDS             complement(33290..33574)
                     /locus_tag="U2I75_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695486.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(34402..35523)
                     /locus_tag="U2I75_000324"
     CDS             complement(34402..35523)
                     /locus_tag="U2I75_000324"
                     /inference="COORDINATES: protein motif:HMM:NF019371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEN1695487.1"
                     /translation="MAKLKLQLEEEERVNQVSEELSEEIKDLCKKAKNKEIFSITDQI
                     KYARFKGIEISESDESIVQDILLYRTYYFKVTAYRKNFEKDINGKYIDLSFVKLADLA
                     KIDMYLRMILSRMIFELEHSLKTLIISSITNSLTEDGYKIVEDYDMYMQNKFIDSQRK
                     KGNTKTNEEILSEYTQASEKILDKVKGKFGYDFDFYNHHHDKISIWALLEVMTLGNLE
                     RFITFYFEEEHFGYLGLKTAQKLLKYVTNVRNASAHSRPLIYNIVEPYQFGRADSNLK
                     KRRPVIQLTQFATNAGLDSELIARALTNMKMNDIVATIYLHEKYVQTQKLKTTNFEAL
                     SDLLDRIKVNGKMYEKNHELVEVFHFFEKIVMEYGKLIT"
     gene            complement(35573..37189)
                     /locus_tag="U2I75_000325"
     CDS             complement(35573..37189)
                     /locus_tag="U2I75_000325"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEN1695488.1"
                     /translation="MSNASEITAKLWAMANKLRGTMDASEYKNYILPFMFYRYLSENQ
                     DEYLEKNGLDEYYEVTDSEEKEDYLEEISRGIGYAIAPEYTWEQLVSKIENHQIKASD
                     FQDMLDSFNLNAKRNPVAEDDFANVFSDINLGDTRLGSNTNERAKALNDVVLMVNDFT
                     FKDDSGHDILGDVYEYLIGQFAANAGKKGGEFYTPHEVSQVLAKIVTKDAKADDTQFR
                     VYDPAMGSGSLLLTVKNELPDGNKQGSVDFYGQELNTTTYNLARMNLMMHGINYRNMN
                     LKRGDTLDADWPFAEKDGMQIPLKFDAVVANPPYSAKWDIKNVDREKDTRFKGYGVAP
                     ASKADYAFVLHGLYHLDKTGTMAIVLPHGVLFRGASEGRIRKNIIDNNLLDTVIGLPA
                     NLFYGTSIPTAVLVFKGRESRRTKDILFIDASSEFVKGKNQNKLSPENIDKIIETYEK
                     REDVEKYAHVATLDEIKENDYNLNIPRYVDTFEEEEVTPLPHLAQELADVKQEIEKST
                     DKLFEMLGELHGTTDEAQEELRKFMEMLGR"
     gene            complement(37205..38419)
                     /locus_tag="U2I75_000326"
     CDS             complement(37205..38419)
                     /locus_tag="U2I75_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN1695489.1"
                     /translation="MSNEITKEPEIRFDGYEGDWEERKVGEVFSERSERSGEGELISV
                     TINSGVVKASELDRKDNSSDDKSNYKKVEVGDIAYNSMRMWQGASGYSPYNGILSPAY
                     TVIIPKKDIDSKFFAYDFKRYDMIQTFKRNSQGLTSDTWNLKFPTLKSVKVMVPSIEE
                     QKQISTFFESLDTTITLHQRELDLLEQTKQTYLKKMFPKTGEDKPEIRFAGFTDAWEQ
                     RKLGEVATRVTRKNKKLESTLPLTISAQFGLVDQNTFFNKQIASKDVSGYYLLKKGEF
                     AYNKSYSNGYPWGAVKRLDKYDMGVLSTLYICFRPVDINSDFLASYYDTDRWYKEVSK
                     HAAEGARNHGLLNIAASDFFETELMIPKNNNEQEKIADFFKSLDTTITLHQRELDSLK
                     EMKKTLLQKMFV"
     gene            complement(38416..39417)
                     /locus_tag="U2I75_000327"
     CDS             complement(38416..39417)
                     /locus_tag="U2I75_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEN1695490.1"
                     /translation="MNEIQFLIYEDSDKVEVLIREETIWATQKTISQLFDVGVPAISK
                     HLKNIFESGELDEEVVVSILENTTQHGAIDGKTQTNKVKYYNLDAIISIGYRVNSQKA
                     TKFRIWATDILKDYIQKGFKIDVERMKQGENAFGKDYFRELLETVRSIRASERRIWQQ
                     ITDVFAEISYDYDKNSEITKQFYATVQNKFHYAITGKTAAEIVFNGADKTKENMGLTT
                     WKNSPDGRILQSDVSVAKNYLSEKQIRSLERNVSSYFDYVERLLEDETLLGMEDFAKS
                     IDEFLTFNRYQILDGNGKISSKAAKRKAVDEYKEFNKHQHIVSDFDKEITKIEGELK"
     gene            complement(39424..42498)
                     /locus_tag="U2I75_000328"
     CDS             complement(39424..42498)
                     /locus_tag="U2I75_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN1695491.1"
                     /translation="MTKQFGIKSQSELRFEQEVVDYLTRIGGVKQWEYKAEIKTTEQL
                     WNNFKKILEQNNQARLDEPLSATEFNQVKKIITNINTPYQAGQFLYGVNGVSEIEVDL
                     DNGKHVFLTVFDQAQVGGGNTVYQVVNQIQRPKVVDGKPNRRFDITLLINGLPIIQIE
                     LKKALHNATEALNQMEQYIAEKQFSGIFSTLQILIAMTPFDIRYMANTTLQNFNRAFA
                     FNWQDEETAHPVRSWKLFADKVLSIPMAHDLSTRYMVLDGTKNKESIKVMRPYQVYAT
                     KRVLDKVRKFDFKHDEGKLGYIWHTTGSGKTITSFKTAWLASRLSNVDKVIFLVDRIA
                     LTNQTADAYRAYDPIAGFEGKTGVVSDTANISDLHRKLTKKSDKNIIVTSIQKMSRYV
                     TRDSFQPLTENVLFIVDEAHRSTGDGTKNEGMLESIRKALPNSAWVGYTGTPKFPETR
                     EIFGDLLHAYTIKEAIADKNVLGFKVEFKETIEAPADPTEEDIDDNLRGSVYDHSPEH
                     VELVVEDIFDNWKERSNNRKYNALFTVHVGGNRASTPRAMEYFDKFMEENNKRSIEDQ
                     IKVAISFSMDTSNSSHQLKTNENLHRAIQSYNRLFGTAFDMTTVKSYTEDLARRLNKT
                     ADDGNYLDLVIVVDQLLTGFDAPELNTLYIDRTLKGGNLIQAYSRTNRIHDRDAKPQG
                     NIVNYRWPIQNEYEMNKSFAIYSNRDSAAEQLSLDELKKDNEESGILSKSFSKIESET
                     KEVIQKLAELTDNFIQLPPSEKAQEEVFEGLQEYNRLLNQLKQFTEDDEKNPVSVYDE
                     PELFYDRLGITEDQEVMLTTVLANELRERRAQIENIDISQVNLEMTHIHDVTINYDYL
                     IELIARMADEVHEDKMDQAELTKNEIDVEIAKSDSDKEITKVRNFVSKIFTKEFIFDK
                     YPAPRDVEEMNQAMEQSQKDSNIKLYTEFVRTWGLDNSVKPKDLAKLISRHRVGQEDM
                     DKQGELTAMMNDARTDYKEIAAAEVANLSWVKYRIEFRKAFYQMADETKKGE"
     gene            complement(42502..43080)
                     /locus_tag="U2I75_000329"
     CDS             complement(42502..43080)
                     /locus_tag="U2I75_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN1695492.1"
                     /translation="MEMRKLSEFVTFVQGINQSRAEKQFGNQEIDYYDQASFEEDHKY
                     YNEATQEERVNYLFDQEVSLDKRDVVISNSLQRATMVSGQNVGKVLSLNFTKVEFKSD
                     LLDKRYFLYLFNNHKDIQRQKDRGLQGTGPVLRLTKQSLEQLVIPLPLIEEQRKIGGI
                     YTESLKLQSKLSIYTRLLEQFTNSMLEKSLEE"
     gene            complement(43383..43628)
                     /locus_tag="U2I75_000330"
     CDS             complement(43383..43628)
                     /locus_tag="U2I75_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1695493.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRIRLEKGIGQTELIRDLQLHKIA
                     ITRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            43733..44506
                     /locus_tag="U2I75_000331"
     CDS             43733..44506
                     /locus_tag="U2I75_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695494.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSETSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAKLYTEKVEQFFKSGANKDFSKISDYSENFFNETQDEIMNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLEKPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEVEMVIKIF"
     gene            complement(44736..44939)
                     /locus_tag="U2I75_000332"
     CDS             complement(44736..44939)
                     /locus_tag="U2I75_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HEN1695495.1"
                     /translation="MLQEVVHSNSEIEITRDSKNGLNDGVIMISKRKWELLQEELYLQ
                     RTGTWAYVSDLMKNEKEDDFKVV"
     gene            45020..46146
                     /locus_tag="U2I75_000333"
     CDS             join(45020..45248,45248..46146)
                     /locus_tag="U2I75_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1695496.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNTLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(46334..46684)
                     /locus_tag="U2I75_000334"
     CDS             complement(46334..46684)
                     /locus_tag="U2I75_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695497.1"
                     /translation="MIFENESEEIGRMTEENKVDLLETLVINKKEFEQLFLDFFVQYD
                     RIYDFNSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDADYYQPQEVFR
                     EFRNKIIGFSQTTK"
     gene            complement(46725..47219)
                     /locus_tag="U2I75_000335"
     CDS             complement(46725..47219)
                     /locus_tag="U2I75_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695498.1"
                     /translation="MFKVYFPFQWLVKGIFFVLLGLILFGSYLTIKARYFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESGKSETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFNL"
     gene            complement(47212..47514)
                     /locus_tag="U2I75_000336"
     CDS             complement(47212..47514)
                     /locus_tag="U2I75_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695499.1"
                     /translation="MSDPYIKVYCSKETKKLFDSFYEKNKGTLGTKANMFRVMVSNLP
                     MLASQSNKKFSDPESIKLEQTISELESSIMKKLDGIDQKLSCSLKTKYKTEEKKDV"
     gene            complement(47537..48874)
                     /locus_tag="U2I75_000337"
     CDS             complement(47537..48874)
                     /locus_tag="U2I75_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1695500.1"
                     /translation="MGSIIKIASKSRSIHNTISYMLNKQEDSELVTGIGVSNDLNAIE
                     EFKRTNEIFGDKGKIKLWHFTQSFDPLEDISAEKAHELGVKWAEKMFPNQSILVVTHT
                     DKGHVHNHFGISPNDQRTGKKMDIRKNDIRVLQAWNDNILIDNGINPLDMYKYNHLVA
                     DRENWKEREFRTENKISNREKIKLAVSEVAQYAKNYDDFHAELKKNHSIETYRFAKNQ
                     RIGYKLLDSEDGLILKIGGKRLGQNYDMNYLDDLFTENKKAEQIQSEEVLTEIAIRYL
                     GKSLNGRADSRFNWQERAQRFELDNENIPFLRSSKDYEEFYDKYKFEIEDRIENLLDV
                     FGENKNQFFGFAVEEEPLKYKKKCVNLVYKKVVYDIAESLSTDYDQFNIQDTEPALKR
                     NFKKRYIQETKRVLYLFNRAIDDGTIEKARREYEETARGIDYQKQQNQYDMDF"
     gene            complement(48859..49356)
                     /locus_tag="U2I75_000338"
     CDS             complement(48859..49356)
                     /locus_tag="U2I75_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695501.1"
                     /translation="MAIDVSKLPLEERRKRYKRNEDSRRQNRPIKFRVDDYQYQELEK
                     TADIFGLSVPMYVKKIAVDNAGKSLKYTDRQYKEINFHLLAIGRNLNQLVKTANYLRV
                     QLNKKDTEIQKEEHQSVYLSFTTKQERLKRLYAEKNDLMIQIKSISDPLNRLEKELNK
                     LWARL"
     gene            complement(49505..49843)
                     /locus_tag="U2I75_000339"
     CDS             complement(49505..49843)
                     /locus_tag="U2I75_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695502.1"
                     /translation="MARKKQKSIADLKKELAELERKLYPELGKYVYEKTGISSVKEFE
                     ETFEINQKGTQEKWQKAVNFANSEFTLVHEFLASDQLMETKFQQFLRRKGETAYRIFY
                     REEAQEESVR"
     gene            complement(49847..50032)
                     /locus_tag="U2I75_000340"
     CDS             complement(49847..50032)
                     /locus_tag="U2I75_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695503.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     VMKNELMNELSHIQKGA"
     gene            complement(50046..52799)
                     /locus_tag="U2I75_000341"
     CDS             complement(50046..52799)
                     /locus_tag="U2I75_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695504.1"
                     /translation="MKIIKKKIDNRKINWLLISFLILIAMIIFTSNVAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQSVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMINKT
                     NDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVATKFSDN
                     FLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLNAGEGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERRLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTMSAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENPQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVDVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSGTSQPRTVDIRENLQNTTSSQNGNRTVDVREN
                     VQSSASNQPRTIDVRENLQNTTSSQSGNRTVDVRENVQGSASNQPRTVEVRETRAEAS
                     QGDKTQKINIVEERKERPNEKVIVEQEVKEKKRQRFTYENNPLFQDISDKPNPLFDSL
                     FKK"
     gene            complement(52786..53004)
                     /locus_tag="U2I75_000342"
     CDS             complement(52786..53004)
                     /locus_tag="U2I75_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695505.1"
                     /translation="MIENHLVDKIYCLDHNADTLELIESICSNTIVLDLRAVNDDYVT
                     HIIVNKKMAQKLSESLKKWAEGGNNENN"
     gene            complement(52997..53737)
                     /locus_tag="U2I75_000343"
     CDS             complement(52997..53737)
                     /locus_tag="U2I75_000343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695506.1"
                     /translation="MREKLKKLGDKKRHTFQGTFEFNGYKKDKYKFGQEYENATFMLS
                     NLKIVQGDNLELVTDHIWMNLTKQFLKFGWLKKGDMVQFDGRIKSYSSKKGINYKIER
                     PSKVKVFRKGTEINESSALKISTRYEIVEDIKRQNREYYEARDFFFENYFSVPRYFKL
                     KEKWPEIQAVVAINENRYTSEQFKQVFKDRDSLVDLYKKIQETDKYNLQKEFYNGLVS
                     NKNRIDLNEVEGYLHKIENFDYGRGYFD"
     gene            complement(53870..54745)
                     /locus_tag="U2I75_000344"
     CDS             complement(53870..54745)
                     /locus_tag="U2I75_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HEN1695507.1"
                     /translation="MAEKRMLAKSFLNNASFMKMSDSAKLLYVYLNTNADDDGIVEAY
                     PYTKMLGTPDDDVKILFTKGFVFFLNEDLLAFLPHWLQNQNIRIDRYKPSIYRFKLLN
                     LKPEFFSKLKPIKFDMILQSGQVEPLRVDLMEKWYPAGIPCDVKSDTQLSQDQNSQDQ
                     TNQDKDSIEEINQDQNSFFENIINQKGIQIITRLGDRYTEVQREKYLKLIASMKDSIE
                     REYKIEIRGQDYFDITNKMLTRLFNSINEKLNLGEEIDEMKFINTTARNHWEDIAKQE
                     KASRLDMLETLKKIG"
     gene            complement(54915..55196)
                     /locus_tag="U2I75_000345"
     CDS             complement(54915..55196)
                     /locus_tag="U2I75_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695508.1"
                     /translation="MEQIGVSVMLNTDQTNQLKTHVFEVTKEAIDQARIDSGLERPFL
                     KGKEMAKFLNVSYGTFLKFKRLGLPIIVVDTIELYSKEEVKKWMLQHQI"
     gene            complement(55251..55448)
                     /locus_tag="U2I75_000346"
     CDS             complement(55251..55448)
                     /locus_tag="U2I75_000346"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1695509.1"
                     /translation="MFQINEDFSIRIRERRGKLMITKTKAAELIGISRQTLKKIECFE
                     VQSVSKNVYQKIIGWLLKSDY"
     gene            55615..55989
                     /locus_tag="U2I75_000347"
     CDS             55615..55989
                     /locus_tag="U2I75_000347"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1695510.1"
                     /translation="MTQKNRIKETREEQNLTLKELSNLSDIPYQTLRNYEIGKRKPRN
                     DEVWWKLGTILNVRGPYLKGEDSFKTNDELHIYYLKKSHTNFELSKKEEIIDIIDKLN
                     EDGLDEALRQLALLSKIDDFKR"
     gene            56079..57245
                     /locus_tag="U2I75_000348"
     CDS             56079..57245
                     /locus_tag="U2I75_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1695511.1"
                     /translation="MSIQKYKTKSGTRYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDMGYHRPSNDLFRTVYEEWLPSYKNRVQETTYQRTKDLFRLHILPKFG
                     DKSISKITPIYCQNAINEWAVKYTNIKQLKSYSSQIFEYAIFAELLQRNPMTNTKLPK
                     QEKKESENYFSLEELKEFLEILNKEESLKHILIFQLLITTGVRKGELSALRWADINFE
                     EQLLHIGKSYATIRTEDPNATRKTIRIQKNTKNVSSERILPIDTQTIEMLKLWKKEQA
                     EVLLQFGINANTKKQLIFTYVNANGDVNQPLHADYSNNIMRRLEKKYKFKHVTIHGLR
                     HTHATLLLEGGASIKEAQDRLGHKNAETTLNTYSHVTEKAQRNAVDKFIAYTGL"
     gene            complement(57344..57736)
                     /gene="rpsI"
                     /locus_tag="U2I75_000349"
     CDS             complement(57344..57736)
                     /gene="rpsI"
                     /locus_tag="U2I75_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1695512.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(57752..58195)
                     /gene="rplM"
                     /locus_tag="U2I75_000350"
     CDS             complement(57752..58195)
                     /gene="rplM"
                     /locus_tag="U2I75_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN1695513.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(58714..59181)
                     /locus_tag="U2I75_000351"
     CDS             complement(58714..59181)
                     /locus_tag="U2I75_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN1695514.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(59301..60839)
                     /locus_tag="U2I75_000352"
     CDS             complement(59301..60839)
                     /locus_tag="U2I75_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN1695515.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            60919..61605
                     /locus_tag="U2I75_000353"
     CDS             60919..61605
                     /locus_tag="U2I75_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN1695516.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(61806..62333)
                     /gene="msrA"
                     /locus_tag="U2I75_000354"
     CDS             complement(61806..62333)
                     /gene="msrA"
                     /locus_tag="U2I75_000354"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1695517.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(62417..63094)
                     /gene="pgmB"
                     /locus_tag="U2I75_000355"
     CDS             complement(62417..63094)
                     /gene="pgmB"
                     /locus_tag="U2I75_000355"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1695518.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(63091..64221)
                     /locus_tag="U2I75_000356"
     CDS             complement(63091..64221)
                     /locus_tag="U2I75_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1695519.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(64221..66404)
                     /locus_tag="U2I75_000357"
     CDS             complement(64221..66404)
                     /locus_tag="U2I75_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1695520.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(66401..67426)
                     /locus_tag="U2I75_000358"
     CDS             complement(66401..67426)
                     /locus_tag="U2I75_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1695521.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(67471..68298)
                     /locus_tag="U2I75_000359"
     CDS             complement(67471..68298)
                     /locus_tag="U2I75_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1695522.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(68295..69362)
                     /locus_tag="U2I75_000360"
     CDS             complement(68295..69362)
                     /locus_tag="U2I75_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN1695523.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(69388..70224)
                     /locus_tag="U2I75_000361"
     CDS             complement(69388..70224)
                     /locus_tag="U2I75_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1695524.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(70221..71090)
                     /locus_tag="U2I75_000362"
     CDS             complement(70221..71090)
                     /locus_tag="U2I75_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1695525.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(71160..72443)
                     /locus_tag="U2I75_000363"
     CDS             complement(71160..72443)
                     /locus_tag="U2I75_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1695526.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(72457..74142)
                     /locus_tag="U2I75_000364"
     CDS             complement(72457..74142)
                     /locus_tag="U2I75_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN1695527.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            74417..75442
                     /locus_tag="U2I75_000365"
     CDS             74417..75442
                     /locus_tag="U2I75_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1695528.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(76037..79711)
                     /gene="rpoC"
                     /locus_tag="U2I75_000366"
     CDS             complement(76037..79711)
                     /gene="rpoC"
                     /locus_tag="U2I75_000366"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN1695529.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(79769..83386)
                     /gene="rpoB"
                     /locus_tag="U2I75_000367"
     CDS             complement(79769..83386)
                     /gene="rpoB"
                     /locus_tag="U2I75_000367"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1695530.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            84114..85082
                     /locus_tag="U2I75_000368"
     CDS             84114..85082
                     /locus_tag="U2I75_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1695531.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            85100..86005
                     /locus_tag="U2I75_000369"
     CDS             85100..86005
                     /locus_tag="U2I75_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695532.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            86002..86808
                     /locus_tag="U2I75_000370"
     CDS             86002..86808
                     /locus_tag="U2I75_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1695533.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            86944..87933
                     /locus_tag="U2I75_000371"
     CDS             86944..87933
                     /locus_tag="U2I75_000371"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN1695534.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(88250..88774)
                     /locus_tag="U2I75_000372"
     CDS             complement(88250..88774)
                     /locus_tag="U2I75_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN1695535.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            89061..>90125
                     /gene="scm"
                     /locus_tag="U2I75_000373"
     CDS             89061..>90125
                     /gene="scm"
                     /locus_tag="U2I75_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1695536.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        26429 a        18758 c        15545 g        29393 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gttttttcaa
     4441 accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta
     4501 actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt
     4561 taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac
     4621 tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt
     4681 ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtaa
     4741 atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag
     4801 gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac
     4861 gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga
     4921 tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg
     4981 agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat
     5041 ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg
     5101 ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc
     5161 tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac
     5221 aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc
     5281 aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa
     5341 cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg
     5401 aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta
     5461 gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg
     5521 ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg
     5581 atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag
     5641 aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc
     5701 attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt
     5761 actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg
     5821 aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga
     5881 tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta
     5941 aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg
     6001 aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt
     6061 tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa
     6121 aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat
     6181 aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat
     6241 cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg
     6301 agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct
     6361 catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac
     6421 ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa
     6481 taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa
     6541 ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc
     6601 catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt
     6661 catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc
     6721 attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccgg
     6781 tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt
     6841 cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg
     6901 tagctgagtc tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa
     6961 ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata
     7021 gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg
     7081 atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata
     7141 agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc
     7201 tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc
     7261 ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc
     7321 atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt
     7381 ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc
     7441 tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg
     7501 ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat
     7561 ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa
     7621 atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact
     7681 ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag
     7741 ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc
     7801 acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac
     7861 gtagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct
     7921 attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac
     7981 tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac
     8041 atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt
     8101 gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc
     8161 atcttccctg attcgtaagc ttggttatag atttcttcgc catattcgta acattgcata
     8221 tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc
     8281 aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggttgc
     8341 atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt
     8401 gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa
     8461 aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag
     8521 ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc
     8581 cggggatagc acggaacctg tatctttatg gtccatttca caatgctcga ttttcttctg
     8641 ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga
     8701 aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg
     8761 caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat
     8821 ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc
     8881 ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt
     8941 tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt
     9001 caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa
     9061 ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat
     9121 tgattcttat caaactatag aagatagctc ttctgcagta gaaaatttta caccgccaaa
     9181 aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc
     9241 ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt
     9301 gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt
     9361 tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata
     9421 accataaaat cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt
     9481 agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat
     9541 tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa
     9601 agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa
     9661 acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc
     9721 tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg
     9781 acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag
     9841 attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg
     9901 acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga
     9961 atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa
    10021 tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt
    10081 cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa
    10141 tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc
    10201 ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga
    10261 ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata
    10321 gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc
    10381 atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga
    10441 tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat
    10501 tgtattttct gagacagtag ctgactgcaa agtactctct gtatcgtttt caattcctgt
    10561 ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt
    10621 tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt
    10681 catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt
    10741 aaaagcaagt caatgtccat atttaaataa catttgtttc tttttgataa atttgctttt
    10801 tctacataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt
    10861 cgtttgaata actatttaac aagtaattct ctttggatat tatccgttat ggattatact
    10921 tcctgtgtcg tcggacggat aatcatgtta ctgacagaca tccggtctgg cgtttctatc
    10981 gcaaatgcta cagcagaagc tatatcttcg gaggtcaatc cccattcttt ttgcgcttca
    11041 tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct
    11101 cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc
    11161 atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca
    11221 tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga
    11281 agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat
    11341 tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat
    11401 acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa
    11461 tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct
    11521 tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc
    11581 ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat
    11641 aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc
    11701 ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt
    11761 cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc tgcaacaatg
    11821 gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt
    11881 ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg
    11941 ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca
    12001 cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt
    12061 ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta
    12121 atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct
    12181 gctaaaggct cttcgttttc agcttggatc tgatggatca cttcaaagag cggatccgtt
    12241 actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct
    12301 tttatcctta ctttataacg gacggcttat catgtctaat acttataaaa catacttaac
    12361 tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat
    12421 attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca
    12481 aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa
    12541 cttggtgtga cacttttcac tcggggctcc cgagagatac agttgacgga ttctggtcga
    12601 tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta
    12661 acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc
    12721 tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat
    12781 ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt
    12841 ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa
    12901 gatcgttggg gacttttgat acatcgaaat catcctttag caaagcaaac cgtcatccat
    12961 ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa
    13021 ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta
    13081 ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg
    13141 atcatcaata cttatgggac ggaaaaaata ttcatcccac tcgatccgcc attacaagct
    13201 tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta
    13261 gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta
    13321 acaacgaata agataagcaa aagatccaac gattaaataa cataagtttc ttaatttatt
    13381 tcttcctttt atttctgata attccagtaa attactataa ttataaaaaa agaattgaca
    13441 aaacataact gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga
    13501 tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct
    13561 atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg
    13621 gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg
    13681 gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga
    13741 tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc
    13801 aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc
    13861 atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc
    13921 acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa
    13981 gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc
    14041 tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata
    14101 ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg
    14161 tatctgacat catccattta gtggaatcag aattggaaac agatatccat tcagaaccat
    14221 tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa cgaacccatg
    14281 cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag
    14341 aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga
    14401 tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg
    14461 tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat
    14521 gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat
    14581 cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg
    14641 taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc
    14701 agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa
    14761 tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc
    14821 atcttttgct cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc
    14881 tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa
    14941 gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct
    15001 cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg
    15061 ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca
    15121 tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt
    15181 tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat
    15241 catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa
    15301 caaagcaaaa acagcttcct tgatgctagc agcagctttt gcttcattct ttactggtgt
    15361 gacagaacca cttgaatttt catttatgtt cgttgcttgg ccattatatg tcttgcatgc
    15421 aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc
    15481 ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc
    15541 attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt
    15601 tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga
    15661 cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc
    15721 tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt
    15781 gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg
    15841 tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt
    15901 tgcagatgag atggcaaaac tacatggtgg tgctgcagca cgacctgcac aaacaaacac
    15961 cgtggttaaa acagaaacat ttgccggtga aacgatcgaa caagatattt acgctatcgc
    16021 aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat
    16081 gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac
    16141 gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga
    16201 agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata
    16261 tgtagaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc
    16321 tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt
    16381 ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt
    16441 ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag
    16501 ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa
    16561 ctccgcggta ataagtccag ctaaacgaaa catatgaaga gctatttttt atttgactct
    16621 tcttattact tgatgcagaa cagctttttc acaaaatttt tcttcatact caaaactaat
    16681 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
    16741 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
    16801 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
    16861 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaactct
    16921 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
    16981 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
    17041 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
    17101 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
    17161 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
    17221 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
    17281 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
    17341 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
    17401 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
    17461 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
    17521 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
    17581 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
    17641 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
    17701 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
    17761 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
    17821 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
    17881 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
    17941 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
    18001 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
    18061 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
    18121 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
    18181 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
    18241 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
    18301 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
    18361 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
    18421 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
    18481 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
    18541 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gcttacaaca
    18601 agatacgttc cttttgggaa tgggatgatt cgcgtagtgt ttggaacatc ctgactcgtt
    18661 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
    18721 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
    18781 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
    18841 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
    18901 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
    18961 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
    19021 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
    19081 tcgactaacc cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
    19141 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
    19201 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
    19261 atcaaactgc ccttttcttc caagcggtct ccatcaaatc cataagaaac gttgattact
    19321 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
    19381 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
    19441 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
    19501 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctactgatt
    19561 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
    19621 tatccatatc taaacgccgt ttctgtcact cttgatccat ctagaagatc gcgattagcc
    19681 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
    19741 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
    19801 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
    19861 atcttcctcc ttgattgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
    19921 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
    19981 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
    20041 aaattttggg gaagacagat tctaagtaaa caaagcggta aaaatatctt ttattccctt
    20101 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
    20161 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
    20221 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
    20281 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
    20341 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
    20401 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
    20461 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
    20521 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
    20581 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
    20641 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
    20701 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
    20761 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg cactttatcg
    20821 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
    20881 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
    20941 tcatggagat gcgaaagttc atccaagaag cagttgaatc gcttattcag caaaatgcct
    21001 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
    21061 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
    21121 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
    21181 tcgatcatac tgacgcctct tattcaaaac gctactctat aaaaatcctg aaagaagact
    21241 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaacta aagaaacggt
    21301 aaagaaagac gatttcaatg aaaaatagca agtaaacgat aacaaccatc ctaaatccac
    21361 aagaaataca gcagaccttg ctttaaaaac acgaatcatg caaaggatga agtaaagaag
    21421 acttaacgcg tacccaatat tagtcatttg actattaggt acgcgttttg ttttataagt
    21481 catgacaaat ttccttcgtt gctggataga ttttccggca ttgttgatag attttatgat
    21541 acttttctac attttgtaag agaggttccg ctgcatctgt atattggata aaattctgac
    21601 aacaactttc aggtgtgtcg aaccacccta accctagtgc agcaatcatt gttgcgccta
    21661 atgcaggtcc ttcctccata cttaaacaat agaccgttgc atcgaaaata tcagcttgga
    21721 tttgtagcca tgcaagattt ttcgcgcctc ctcccaccga aacaatacgt gtaaatctgc
    21781 gacccgctag ttctttacgt ttcaatccca ccattgacaa atgctcatta aaaatttaga
    21841 atattcacaa gtttaacgac aattatgaaa atttatgtcg tgctaaagga gttttgttat
    21901 tggatgaaaa gttagatata aaaactgcga tcaaagacac gttacctacc gttttcggtt
    21961 atatcggtat tggacttgca tttggtatcg ttgggaaagc tgccggattt catccactag
    22021 tcgtcacgtt gatgtccctg ctggtctatg ctggttctgc ccaatttatc acagtcagca
    22081 tgcttgctag tcacagccca ttgctttcca tcgttttctc gacctttcta gtcaattccc
    22141 gaatgattct gatgagtatg acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa
    22201 atctgctgat tggcacattg ctgacagatg aaagtttcgc actgggaatg aataaattga
    22261 actatactgg acaaaagtta aactttcgtt ggatgaatac agctaattgg atttcttatt
    22321 tgacgtgggt gggttcttct cttgttggcg cacttttagg gaattttatt actgatccga
    22381 aaaagtttgg tttggatttc gccatcgtcg ctatgtttat cgggttactt tatcttcaag
    22441 tcatttctga tagaaacacc tctaaacgat tacagttgat cctgattggt ctcactttga
    22501 ttctggtata tgtcggactg atttttatcc caagcaactt agtgattgta gttgtaacac
    22561 tgattgggtg cggattgggg gtgtggatca aacatgcctt cttttgagtt tacattgctg
    22621 actattttag gttgtacttt agcaacttgg ctttctaggg tcctaccatt tgtcttactg
    22681 aaaaaattcg atttaccgca accgttgctt gagtatttaa gttttgtccc gatcgtcatt
    22741 atgtctgctt tatggttcag cagtctattt acccaaaata tcggacatct gccgcagatc
    22801 aatctcgaaa atgctttggc ttcagttcct acacttttag ctgctattct ttcaaaaagc
    22861 ttattggtga tcgtcttggc aggaattctt tccctttcgt tgattcgctt attataaata
    22921 aaaaaacaag agcttatggc aaattactgt catgcgctct tgtttttatc aaagtcaaat
    22981 aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg accactaaca acgacttttc
    23041 ctaacatatg tcctgtttct acgtctttgg ttgcttgttt caagttcttc gcattcatcc
    23101 catctgaata gaccttgctc aatgtactgc ggataatccc ttcatttgct aaagcagcaa
    23161 tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca
    23221 tatactccca gtcgaagctg atagattggt ttttccaaac acctatatct aacggctctt
    23281 tgatccctac gatcgttccc acatgtccaa atggtcggac aaggtgcttg atttgatcaa
    23341 agtagcgggt aatatcgaat aatacagcga catactctac ttgatcgatt cctaattcac
    23401 gaaaactttt acttaagtcg tgatgataat cgattggata gtctacacct gtttttttca
    23461 gccattcaaa attcttcgga cttgcagtcg cgtagacttc taggccagcc caatgcgcta
    23521 gttgattcaa aatcgaaccg acgcccccgg atccgttgat cactaagatt tttttcccgc
    23581 gattggcatt ttcttctgga gttagaccaa atttttcaaa taacaattca taagcggtca
    23641 atgaggtcaa cggcaaggct gcagcttctt catctgaaag attttttggt gcaagagcga
    23701 caatacgttc gtcaactagt tggtattctt gattgcttcc tgctcttgtt gttgtacctg
    23761 cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc gtcacctaca gcaacaactt
    23821 tcccgactcc atcaaaacct aagatacgta aagcatttct tatcccgtta tcttggcgaa
    23881 gtttcgtatc gacaggatta acagatactg cgttgacttt taccagaaga tcccgtgaac
    23941 tgggtacagg tatctgttct ttttcatcaa taaagctttt tggatcatcg atcggcaacc
    24001 cttcataaaa accgactacc ttcattaatt ccttttctga catacgaatt cctccttagc
    24061 ttcatcaagt tggagcatcc atacagtgtt catttcaata tatatagtat gctatttgtt
    24121 ccaccttttt tattcatcat aattattctc ttacttaaag taaactattt gcactttttt
    24181 ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttataaacg gtgttctcct
    24241 accaactctt cggtcacaag tcaaaatatt catcaaacat gagaagctgt tttttgaaat
    24301 tgctccttgt gactcagagt taaacggcag gctcacaacc tctgatataa acggtgttca
    24361 atcaactgac cctcggtatt agctcaaaaa acgacaaaat atgaagagct atttttgttt
    24421 ggctgttctt attacttgat acaggacagc tttttcacga cctctaattc acggtgttca
    24481 agcaagaaat cattgttcac tgaaaagcgt tggatgttct ttttcaaagg tctctaagat
    24541 ctctcgtact tcatcagtag atttcgtatt catcaactga actcgcaagt catttgctcc
    24601 tggaaatcct ttgatataga ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat
    24661 gatttctgaa taatgatctt gcagatctag ttgcagacga agtagtccga tcagttcttt
    24721 ttctgtatgt tccctcgggt ctttttcaaa tgcataagga ttcttgaaaa tccctcggcc
    24781 aatcatgatg ccatcaacgc catatttctc ggccaattct tctcctgtct ggcgatcggg
    24841 gatatctcca ttgatcgtga ttagtgtttg tggagcgatt tggtcacgta aagcaatgat
    24901 tttaggaatg acttcccaat gtgcatctac tttactcatc tctttacgcg tacgcaaatg
    24961 gacagaaaga ttggctatat cttgatgaag taggtgactg atccattctt ccatctcaga
    25021 catttccgta tagccgatac gcgtcttgac gctgacaggc aaaccgccag ctttagctgc
    25081 ttcaatcaat tgtgccgcta cttctggacg aaggatcaag ccactgcctt ttccacgttc
    25141 tgctacatta gggactgggc agcccatgtt gatatcgatt ccttggaagc ccatttctgc
    25201 catagcaatg ctcatttctc taaaaaattc tggcttgtct ccccaaatat gggcaaccat
    25261 cggctgttca tcttctgtaa atgttaatct tcctcgtaca ctgtcttttc catctggatg
    25321 acagaaactg tctgagtttg taaactcagt aaagaagaca tccggtgctg ctgcatgctt
    25381 tacgacatgg cgaaatacaa catctgttac atcttccatt ggtgctaaaa caaaaaaagg
    25441 ctttggcagc tctttccaaa aattagtagt catttatttg ttactctcca ttctatcaag
    25501 cagtgaagtc gcaatctttc tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag
    25561 caaagtgaac agcttaaaag atgtttctga ttgagcaaaa aaaagactgt gacaaaaatc
    25621 ttatcacagt ccattttccg gaataaatgg tgttcaaaag cttgcatttg cggtaataag
    25681 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
    25741 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
    25801 cgctcaaaaa acaacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
    25861 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
    25921 aataccaagt aacggcgtac aaaacaacga tgaataacac aagtacgatc atcgatgttg
    25981 ctactggcac atcgatcgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
    26041 tcggattgac ttttgaaaca gcttgccaaa agggcggtaa catagagatg gcatagaaaa
    26101 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcatcat
    26161 aagactgagc aaaaatcccg ttgagcagtc ctgctaatga aaacaagatt gccgtcatca
    26221 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
    26281 tgatcgttac gagagagccg acgagaatac tgcgacccac accaccaatc acaaagcccc
    26341 aaataatcac atgcgtagga acaggtgcta ccaagatttc ttcgatattt ttctgaaatt
    26401 tttgagagaa gaaggacgaa gaaacattgg catacgaact agtgatagca gacatcataa
    26461 tcaaaccagg aacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgcccac
    26521 cgatcatttt tccgaaaata acaaaataca aggatgtcgt gatcactggc ggaaccagtg
    26581 tttggaccca tatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
    26641 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaag
    26701 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
    26761 gtaagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
    26821 tgctcatctt caaatgtaca agcatatcct tcaatcacag gtttggtatc atatggcgct
    26881 aaatcaaaga taaatgtttc aaattgcagt tttgacagaa gctctttcat acttgtattc
    26941 tcgatcagtt cacccgattg gatgattccg atgttgcgac aaagcatttc agcttcttct
    27001 agataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg tagaaactcc
    27061 cacatctcgc gtctcaactc gatgtcaact cctgctgttg gttcatccaa gatcagcaaa
    27121 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
    27181 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
    27241 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
    27301 tggaccgttt caaaagggtt aaaattgaac tcttgtggaa ccaatccaat ctgttgcttt
    27361 gctcgaacta gatccttgtc cagatcataa ccgaagacat tcacttttcc agatgtttta
    27421 ttgactaaag aagtaataat cccgatcgtt gtagacttcc cagctccatt tggccctaat
    27481 agcgcataga aatctccttc tttcaccgaa agatcgattc cccgcaatgc ttcgacccca
    27541 cttccataga tctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
    27601 ccttatagac atgattctta attgtatcat ggaatacagc agatgaaaaa acaacttggc
    27661 ttccacacaa aaacacacct gctaacaaag aggagatgtg ttttctagtt catgatcatc
    27721 ttcgatagaa taactatcta aacaactctg gaacttttaa aatcataaaa gtaccgacag
    27781 caactactac gatgacatat agaagatcct tataactgag gttcttgtct tttttattca
    27841 tgttaagctt tcctccgtcc ttttatcgaa agcttttttc ttttatttta accgaaataa
    27901 ataaaatgga aatagttatt ttcaatttct gttttcgctg tttgttttac ggagaacttt
    27961 gccatggaac gtgcgtctcg tcgctgatgc taagtttttt cgataaaagc tgctagaagg
    28021 aagacagcgt ggtttatttt gtatcgtctt ttgctaaatc ttccaagatc atctggacag
    28081 cagactcgtt cccattgaat tctgccactt ttttggcatc aatgtacttc ttttgataat
    28141 cgtctttttg gacccacgca tcatgcttcg tttgataaag ttaatcaaac ggataccact
    28201 gacgctgttc atcgaacaac aaactttctt tttgggcctg ttcaactatg gatttaggat
    28261 aattcaaaac tttcagcaag gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt
    28321 tgaaatcaaa actgattcct tttccttctg ttacttgttc ttcaaagtgg acattctcac
    28381 aataattctt taaaatctct gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg
    28441 gatactccgc aagccaccga acgactgaag cggaagaagc aatccgttcg atcgtatttg
    28501 ttccttttaa aatctcatct acgaagcagt agcagcgttc tttggaagcc acttggtcca
    28561 gcaaacgttt gatcgatttg atttctgaaa caaaataact gtcgccttca aataaatcat
    28621 cttcgattgc catggaagtc aacacatgtc caggttgcag ggtaaactgt tctgcaatcg
    28681 ctgtttggat cgtctgtgct agaatacagc tgatggctat acttttgaca tacgtagatt
    28741 ttcctgatgc attggaaccc gtgactaatg tattcttctg ccagttgact ggattaacta
    28801 ccgcttcttt taacaatgga tgatagatat ctttcgctgt cacaccgccc tcagtaaact
    28861 ctggtcggca agtgatcggc atataggtgc gataattcaa aacggctgct gcaacttcca
    28921 attctcccag caattcccac agcttgatgg cttcagttga atgcttttct aatttagtga
    28981 gaacgaaatg ataggagata aatggcagca tgaacatgat gttgacatat tcaaaaatca
    29041 attctgcttc actcccgctc ttcattcgaa atgaaaaacc taatttaggg atgtctctta
    29101 atggtttagt ttgctgtttc agctcttttt gaagtggtgt cgtcattttt gctaactgat
    29161 tggcggctga aatcgttgaa accaaataac tcatgctatt cagctctgtt tctagtgttt
    29221 gtttcttcac tagataatag accgtattga atactaagcc accgattgct agatagattc
    29281 ctagtacttg ccctaaaaga agcaaaccta cacccaccag cggaagcaag ccaagaaaaa
    29341 taaataaacc tacgcgtcca atctttttac tttttccatc cgctagatat tgttttgcaa
    29401 aattatgatc tttttttcct aaacgagcga attgatattg gattctttct ctcagttgag
    29461 gattttcctg ataaaaagca attagttttt ctaactgata atcttctcca aaatcaaagc
    29521 tacgtaaacg ctgatacaac gcttctgaac ctacactaga ataggtactg ttgatccctt
    29581 caaatacttc gaacatatcc aagtcatacc aagtaagatc gtcgatttca ctatcccatg
    29641 aacggaattt tttctccgtg agccacgcat ccttcaaact ttcttcttta tcaaatctcg
    29701 gttgataagg aatttttccc caattactac gaactgtttc ctttactttc aaacgtgaaa
    29761 aaatgtctaa aacaactaat aaaataacaa ctgcaataat aattaaaacg ataaattctc
    29821 tttggcccat atccctctcc gttccttgat ttgttagttt tagtatagca ctgttgcgct
    29881 atttgaaaag ggggtacaaa aataactgtc ttcgttacct aattcgtatc aatcacacca
    29941 aaataaaaac accttccaaa aaagaagatg tctttaaaac gagatacaat tttttgtcat
    30001 tcacttcagg taaattagag ccaattaaac cctccgcaac ttgacggaga tggagaagat
    30061 gcccccgtct aatctagcgg tcatctctcc tcccattcct tccaccaatt tttttgacag
    30121 gtaaagcccg attccgctag attccgtatt tgtgcggctg gtattttcgg tataaaaacg
    30181 ctggaagact ttttcaacag aggtttgcgc tcctggtttg ataggattgg ccactgttaa
    30241 tcgaatgaat gaatcctctt ttgaaaatgt cacctttgct tgtccatctg cataacgcaa
    30301 taaattctgt atgatatttt gcagcactcg cagcaacatg agcggatctg ctaatacagt
    30361 tgtatttatc gagtgttcag cgaacgctac ctcgatattt ttctcagaaa attgttcata
    30421 aaaagagaaa aatacttctt ccacctcttt agaaaaagaa agcgtatgga gatccatttc
    30481 tttttgcttc gtctctattc gagcaagatc ataaaactga tccgtcatac tgagtaatct
    30541 tgcaacagat tgtttgatga tcgttaaata cttctcccgt ttctccgtat cctctacctg
    30601 ttccagtaat tgtacataac cgttaatagc agtcaaagga gttctgagat catgagaaag
    30661 gccgacgatc gatagtctga tgttttcttc catttcttga aatgcttgtc ctttttgttc
    30721 attttcatcc acccatgtat tcagtgcatc tactaggtcc attagattct tttctcgaaa
    30781 ttcgactgaa agacgaccac catattcttg tcgttcagaa agagtggcga ttttcttagt
    30841 taatctcttg atttcccttt gcaggaagaa aataaaactt cccataaaga taacaacgag
    30901 aagcaacagc cacttaatca aatctaaacc ccactcccca caccgtatga atatacacag
    30961 catgtgcctt tgctagtttc cctcgaagat tggaaatatg catattcact gttttctcac
    31021 tatcaaaata tggttcttgc caaactgttt cataaagatt tgctcgcgaa aatacttttt
    31081 tcggatgttc taaaaaaagc ttcagcagat cgaattctct tcctgtcaaa tggacagcca
    31141 caccattcac atttacatcc ctcgactctt tgtccagtaa gatttccttg taatagatgc
    31201 gttgcgacag atcttttact tggacagaag tctgatgacg aagttggatg ccgattcgta
    31261 acaacagttc ttcgatatca aatggtttcg tcacatagtc atccgctcct gctgccaaca
    31321 aggaaacttt atctctttga tcgctttttg ctgaaagaac gatgatcgga acattcgatg
    31381 tctgtcggat ttctgctaac aatgcttcac ctgaaagccc tggaagcatg agatctagaa
    31441 ggatcaaatc gacaggatat gtgcttaaca ataattttcc ttccgtacct gaataagcat
    31501 caagaagttt gtatctctgt tcaagaacct cctccagtaa agaatgaaca gcttcatcgt
    31561 cttcgataat caaaatagtt tccagcagga acacctactt aatgtcagat ttttggaaaa
    31621 taaacattcc tagagctgat gataccacga ttgttactag actgaccaga atcattttcg
    31681 gtgcttgcga ataagttgct tgttgcgcaa agtccgtata catgagccac gtttccgtaa
    31741 accactccgt tagaaattcc aagcctttga tttttgcgaa aacaccggga acgaaggccg
    31801 ttcccatgat ataaagaaaa gaaagaagca aactgacact ggtacttcta gtgatgaatg
    31861 tcaaagccac tggaacagat aatgtcgcta atatcagcag aagcaataca ggaaatactt
    31921 ttagaacata atttccatag tttttgattc cctcaacttc accaaatccg aaagcaagtg
    31981 tgtaagcaat catggctagc cccgtaatca aaaccacgcc aagtgttgtg atcaaaagcg
    32041 taacgagaag tttcgacaca taataagtca ttcgattccg tcctaaagat aacgtattgc
    32101 gaatcgtacc gttttgaaat tcttccccaa tataaaagca cgcaaatgct gcgaaagcca
    32161 gaagaatcca tgcagccatt ttttccacca ttaaaatccc tgttgcaccg ttgatgactg
    32221 tatccgttgc ttggtttaat tcattgccgt attttgtatg catataccat tcgttccaag
    32281 aataaaaaag cggaagtacg accatcagtc ctagtgagac ccacatttta cggctcttcc
    32341 atacttttat tttttcagca gttaatagat taaacatgtg gattgcctcc agttaattgt
    32401 aagaaataat cttccagttt ttgcccaact aggtggatac catccacagg aacttttgcc
    32461 tctaccaatg cttgattgat ggctgctact tggtcaattc cttcaaaaag ctggataaca
    32521 cggctagatt ggacaaaata atccctataa ccaagagagt cgagaaccgt tactgcttgt
    32581 tccgtcgccg aagtgtcaat ttttataaat tgacgagatt cttgtgccaa ttcttctttc
    32641 gataattctt tgatcaagtg tccttcatgc aaaataccgt agtgagtggc gatctgtgac
    32701 aattcatcta gcaagtggct agaaagcaag acggtgatgc cccgttcttt taccaaacgt
    32761 tggatgattt ctcgcatttc tgccatccct gctggatcca tcccattcgt cggttcatcc
    32821 aaaatcaaaa attctggatt cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata
    32881 cctaaagaga aattctttgc tttctttttc cctgtatttt ccaatcgcat cattttcagc
    32941 aggtcttcga tttctctatc cgacacaccg ccgttcgctg cttggatacg taggttatct
    33001 cttgcagtta attctggata aagagcaggg gtttcaatcg attgacccat acgtcgtctt
    33061 gctgcctgca aatccgtcgt tccaaataat tcgatcgaac cttcatcaat cgagatcaaa
    33121 cccataatag ttctcataaa ggtagactta cccgctccat tctccccaat caaaccatag
    33181 atcattccac gtttgatttc gatcgatact ttatctaatg ctttggcagc accgtatttt
    33241 ttggttatac cagttgcttt caagaccgtt tcatttccaa aatccatact tatccttctt
    33301 tcttattgat aagtgaagta tagttgacgg gtataaagaa agcagccaga aatgtataaa
    33361 gaaagtgtaa attttccaat tgaatttcaa gaaaaatcag actgtctcgc agacgtggtt
    33421 gagttgtctg ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca
    33481 tggtaatcgt tgttatcgtt gacatcagac gtccttgtat atgcgttgga acttctcgct
    33541 gaagccagac catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa
    33601 aataatacat gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga
    33661 cttttcacaa tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag
    33721 tcacaatatt tatcaaacat gagaagcaat ttttgtaaat tccttcttat ttcttgaagc
    33781 tgaccacttc tgtcacaacc tcagaagcgg ataaataata ttgtttgatg atactttatc
    33841 tttttcccgt ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca
    33901 tagctaaaaa cacaaaaaag gcactttcca aattgtggaa agtgccagaa aagttaggat
    33961 taaggtaact gcgctcagca ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga
    34021 acgataatcg caattaacgt tttgagaact gtgaagcttt acgggctttc ttaagacctg
    34081 gttaaacaaa cttttgtttt ataatgtttt acagagaaag aaatcgtgta aaatcagcat
    34141 ttaatgattc atataattct acttagttaa ataaagttat atcctgtcat tacctcaaat
    34201 cattaccaca acttggcgag ttggctgatt gaagaatatt ctgtgccaaa taaaaaaaat
    34261 gatgccaaaa ataagtacac tactcaatac ttattttcaa catcattaat cctaaaatag
    34321 cttcgctttt acccgttact atctaccctt tcggaagggg tatttcatct gactaaagaa
    34381 tagcatccaa gataccaata gtcaagtaat taatttacca tactccatta caattttttc
    34441 aaaaaagtgg aaaacttcta caagttcatg attcttttcg tacattttac cattaacttt
    34501 tatgcgatca agtaaatcag atagagcctc aaagtttgtt gttttcaact tttgtgtttg
    34561 aacatatttt tcgtgaagat atatagtagc cacaatatca ttcattttca tattggtgag
    34621 tgctctggcg attagttcag aatcaagacc cgcattggtt gcaaactgtg ttaattgaat
    34681 aacaggcctt cttttcttca agttgctgtc agctctacca aattgataag gttcaacaat
    34741 attataaata agaggtctac tatgtgcaga ggcatttctg acattcgtaa catattttaa
    34801 taatttttga gctgttttta atcctagata tccaaaatgt tcttcttcaa aatagaaggt
    34861 gataaatctc tctaaatttc ctagggtcat aacttctaat aaagcccaaa ttgaaatctt
    34921 atcatgatga tgattataaa aatcaaaatc atatccgaac ttccctttca ctttatctag
    34981 aatcttttca gaagcttgtg tatattcaga caagatttct tcgtttgttt tagtattacc
    35041 tttttttctt tgactatcaa taaatttatt ttgcatatac atatcgtaat cttcaacaat
    35101 tttatatcca tcttcggtta atgaattagt gattgaactt attatcaaag ttttcaaaga
    35161 atgttcaagt tcaaaaatca ttctactcaa aatcattctt aaatacatat caatctttgc
    35221 taaatcagct aattttacaa atgataaatc tatatacttt ccatttatat ctttttcaaa
    35281 attcttacgg tatgcggtta ccttaaaata atacgttcga tataataaaa tatcctgaac
    35341 aatactttca tccgattcag aaatttctat tcctttaaat ctagcatatt taatttgatc
    35401 cgtaattgaa aaaatttcct tgtttttagc ttttttacaa agatctttaa tttcttctga
    35461 taattcttca gagacttgat taactctctc ttcttcttct aactgcaact ttaatttagc
    35521 caatttgtcg cttttaataa aaacacacct cctttatctc tttattttta tactatcttc
    35581 ccaacatctc cataaacttc cgcaattctt cttgcgcttc atctgttgtc ccgtgcaatt
    35641 cacctagcat ttcaaataat ttatctgtcg atttttctat ctcttgcttc acatctgcta
    35701 attcttgtgc aaggtgtggt aatggagtca cttcttcttc ctcaaacgtg tctacatatc
    35761 ttggaatgtt taggttataa tcattctctt taatttcgtc taaagttgct acatgtgcat
    35821 atttttcaac atcttcacgt ttttcatagg tttcaataat tttgtcaatg ttttctggtg
    35881 ataatttatt ctgatttttc cctttgacaa attcactaga agcatcaata aatagaatat
    35941 cttttgttct cctagattct cttcctttga ataccaacac agcagttgga atacttgtcc
    36001 cataaaatag atttgccggc aatccgataa cagtgtctaa caagttatta tcaatgatat
    36061 ttttccggat tctcccttca gaagctcctc ggaatagtac accatgaggc aatacaatcg
    36121 ccatcgttcc agttttatct aagtgataca ggccatgtag cacaaacgca tagtctgctt
    36181 tagaagcggg tgctacgcca taacctttaa accgtgtgtc tttttctcga tccacattct
    36241 ttatatccca cttagcagaa tatggcggat tagccactac agcatcaaat tttaatggaa
    36301 tttgcatacc atctttttct gcgaatggcc agtctgcatc aagggtatca ccacgtttca
    36361 aattcatatt tcgataatta ataccatgca tcatcagatt catacgagct aagttatagg
    36421 ttgtcgtatt caactcttgc ccatagaaat caacactgcc ttgcttattg ccatcgggta
    36481 gttcattttt cactgtcagc agcaacgagc ctgatcccat tgctggatca taaactcgaa
    36541 attgagtatc atccgctttc gcatctttgg ttacaatctt tgctagtact tggcttactt
    36601 catgtggggt ataaaattct ccaccttttt tccctgcatt ggcagcaaat tgaccaatta
    36661 agtattcata cacatcaccc aggatatcgt gaccagaatc atctttaaag gtaaaatcgt
    36721 tcaccatcaa aacaacatca ttcaaggctt ttgctcgttc attcgtattt gatcctaacc
    36781 gagtatctcc aagattaatg tccgaaaaaa cattggcaaa atcatcttcg gcaactggat
    36841 tccgtttcgc attcaaattg aacgaatcaa gcatatcctg aaagtcactc gctttgattt
    36901 ggtggttttc aatctttgat actaactgtt cccaagtata ttccggagca atcgcataac
    36961 cgattcctcg actgatttct tccaagtaat cttctttttc ttcagagtca gtgacttcgt
    37021 agtactcgtc taacccattt ttttctaaat actcgtcttg attttctgat aagtagcggt
    37081 agaacataaa tggtaaaata taatttttat actcacttgc atccattgtt ccacgtaatt
    37141 tattggccat tgcccataat tttgctgtaa tctcacttgc attactcatt aattttctct
    37201 ccttttagac aaacattttt tgtagcaatg tttttttcat ttcttttagt gaatccaact
    37261 cacgctgatg aagggtgatg gtggtgtcga gacttttaaa gaagtccgca attttttctt
    37321 gctcgttatt attttttgga atcatgagtt cagtttcaaa aaaatcagat gcagcaatat
    37381 ttaaaagccc atgattccta gctccctcag cagcatgctt agaaacctct ttataccaac
    37441 gatctgtatc ataatatgac gctaaaaaat ctgaatttat gtcgaccggt ctaaaacaaa
    37501 tatacaatgt tgataataca cccatgtcat acttatctag tcgcttaaca gcaccccaag
    37561 gatagccatt ggagtagctt ttattatagg caaactcacc tttcttcagc agataatatc
    37621 cgctaacatc cttactggca atttgcttat taaaaaaagt attctgatca actaatccaa
    37681 attgtgctga tatagtcagt ggaagtgttg attcaagctt tttgttcttt ctggtcaccc
    37741 tagtggcaac ttcccccaac ttacgctgtt cccaagcgtc agtaaatcca gcaaaacgaa
    37801 tttctggttt atcttcacct gttttaggaa acattttttt taaataagtt tgttttgttt
    37861 gctctaacag atcaagctca cgctgatgaa gggtgatggt cgtgtcaagg ctctcaaaaa
    37921 aggtagaaat ttgcttttgt tcttcaatac taggaaccat tactttaact gattttagcg
    37981 ttggaaattt caaattccat gtatcagatg ttaagccttg tgagtttctc ttgaacgttt
    38041 gaatcatgtc atatcgcttg aaatcatacg caaagaactt cgagtcaata tctttttttg
    38101 gaatgataac tgtgtatgca gggcttagaa tgccattata aggggaatat ccgcttgcac
    38161 cttgccacat tctcattgag ttgtatgcaa tatcaccaac ttcaactttc ttgtaatttg
    38221 acttgtcatc actggaatta tcttttctat caagttcgct tgcctttaca actcctgaat
    38281 taatagtgac cgagataagc tctccttcgc cactacgttc tgaccgttca gagaacacct
    38341 ccccaacctt acgctcttcc caatctccct cgtatccgtc aaatcgaatt tcaggttctt
    38401 ttgttatttc attgctcatt ttaactctcc ttcgatcttc gttatctcct tatcaaaatc
    38461 agaaacgata tgctgatgtt tattgaactc cttgtactca tcaaccgctt ttctcttagc
    38521 tgctttactg ctaatttttc cgtttccatc aagaatttga tagcgattaa atgttagaaa
    38581 ttcatctatg cttttggcaa aatcttccat acctaaaagt gtttcatctt ccaataatcg
    38641 ctcaacataa tcaaaataag aagatacgtt ccgttcaaga ctgcgaattt gtttttctga
    38701 taaataattt ttagcaactg atacatctga ctgtaggatt cttccatctg gagagttttt
    38761 ccaagtcgtc agtcccatgt tctcttttgt tttatctgca ccattaaaaa caatctccgc
    38821 agctgttttt ccagtaatag catagtgaaa tttattttgt acagtggcat aaaactgctt
    38881 cgtaatttca gagtttttgt cataatcata agagatttct gcaaatacat cagtaatttg
    38941 ttgccaaatt cttcgttcac tggcacgaat tgagcgaacc gtttctaata actctcggaa
    39001 gtaatccttt ccaaaagcat tctcaccttg tttcattcgc tctacgtcaa ttttgaagcc
    39061 cttttgaata tagtctttca gaatatctgt tgcccagatt cgaaattttg ttgctttttg
    39121 agaattaact cggtaaccaa tagaaataat cgcatcgaga ttataatatt tgaccttatt
    39181 tgtttgtgtt tttccatcta ttgcgccatg ttgagtggta ttttccaaaa tggaaacaac
    39241 cacttcttca tctaattcac cagactcaaa tatatttttt aaatgcttgc taattgctgg
    39301 taccccaaca tcaaaaagtt gtgaaatcgt cttttgcgtc gcccaaattg tctcttctcg
    39361 aataagcact tcgactttat cactatcttc ataaattaaa aactgaattt cattcaatag
    39421 aacttactcc cctttcttag tctcgtccgc catctgatag aaagctttcc gaaattcaat
    39481 tcgatactta acccatgaaa gattcgccac ttccgctgct gcgatttctt tataatctgt
    39541 tcgtgcatcg ttcatcattg ctgttagttc accttgtttg tccatatctt cttgaccaac
    39601 tcgatgccgt gaaattaatt tggctaaatc ttttggcttc acactattat ccaatcccca
    39661 tgtgcgaaca aattctgtat atagtttgat attgctatct ttttgagatt gttccatcgc
    39721 ttgattcatt tcttcaacat ctctaggagc cggatactta tcaaagatga attccttcgt
    39781 aaagatcttg gaaacaaaat tgcgaacttt cgtaatctcc ttatcgctat ccgattttgc
    39841 aatttcaaca tcgatctcat tttttgtcag ctcagcttga tccattttat cttcatgaac
    39901 ctcatctgcc attcgtgcga tcaattcaat cagataatca taattgatcg tcacatcgtg
    39961 aatatgcgtc atctctaaat tgacttgcga tatatcgata ttttcgattt gggctcggcg
    40021 ttcacgcaac tcgttagcaa gtaccgttgt cagcatgacc tcttgatcct ctgtaattcc
    40081 taagcggtca tagaacagtt ccggttcatc ataaacagaa acaggatttt tttcatcgtc
    40141 ctccgtgaat tgcttcagtt gatttagtaa tcgattgtac tcctgtaatc cttcaaatac
    40201 ttcctcttgc gctttttcac ttggaggtaa ttgaataaaa ttatctgtta gttcagctaa
    40261 tttctgaatc acttctttgg tttctgattc gattttacta aatgatttcg ataaaattcc
    40321 cgactcttca ttgtcttttt ttaattcatc tagcgatagt tgctctgctg ctgaatctcg
    40381 atttgaataa atagcaaaag acttattcat ttcgtattca ttttggatgg gccaacgata
    40441 attaacaata tttccttgtg gctttgcatc cctatcatgg attctattcg tacgtgaata
    40501 tgcttgaatt agattgccac cttttaacgt acgatcaatg tacaatgtat taagctccgg
    40561 tgcatcaaaa cctgttaata gttgatccac gacaatgact agatccaaat aatttccatc
    40621 gtcagctgtt ttgttcaaac gtctagctaa atcttctgta tatgatttaa ctgtcgtcat
    40681 atcaaaagca gtaccaaaca atctattata agattgaatt gctcggtgaa gattttcatt
    40741 tgtttttagc tgatgactac tatttgatgt atccatcgaa aaactaatgg caacttttat
    40801 ctgatcctca attgaccgtt tattattttc ttccataaat ttatcaaaat attccattgc
    40861 tcgaggagta ctagcacgat ttccccctac atgtactgta aataacgcat tgtatttccg
    40921 attattagag cgttctttcc agttatcaaa aatatcttca acaacgagtt ctacatgctc
    40981 gggactatga tcatacacac tgccacgaag attgtcgtca atatcttctt ctgtaggatc
    41041 tgctggtgct tcaatcgtct ccttaaactc cactttgaat cccaaaacgt ttttatcagc
    41101 gatagcttct ttaatcgtat aagcatgtaa taagtcacca aaaatttctc gcgtttctgg
    41161 aaacttaggt gtacctgtat aaccaaccca agcagagtta ggcaaggctt ttcgaataga
    41221 ttctagcatc ccttcatttt tagtcccatc acctgttgaa cgatgtgctt catcaacaat
    41281 aaataataca ttctcagtta atggttgaaa gctgtctctt gttacatatc gagacatttt
    41341 ttgaatactt gttactatga tgtttttatc actttttttg gtaagtttac ggtgcaagtc
    41401 agaaatattg gcagtatcac ttacaacacc agttttccct tcaaatccag cgattggatc
    41461 atatgctcta taggcatctg ctgtctggtt tgtcaatgca attcggtcta ccaagaaaat
    41521 aaccttgtct acatttgata agcgactcgc taaccatgcc gttttaaaac ttgtaatcgt
    41581 ttttcctgaa cctgtcgtat gccaaatata gcctagcttg ccctcgtcat gcttaaaatc
    41641 aaatttccta actttatcaa gtacccgttt tgttgcatag acttgatacg gacgcatgac
    41701 tttgatactt tctttgttct ttgtaccatc caacaccata tatcgagtag ataaatcatg
    41761 ggccatggga atagacaata ctttatctgc aaacagtttc catgaacgta ctggatgagc
    41821 tgtttcttca tcttgccaat tgaaagcaaa tgcacgattg aaattttgta atgtagtatt
    41881 tgccatgtaa cgaatatcaa acggggtcat cgcaatgagt atctgtaagg tagaaaaaat
    41941 cccactaaat tgtttctcag caatatattg ttccatttga tttaaagctt ctgtcgcatt
    42001 atgtagcgcc tttttcaact caatttgaat aatcggtaac ccattaatca aaagcgtaat
    42061 atcaaaccga cgattgggct taccatcaac aacttttgga cgttgaattt gattaacgac
    42121 ttgatagact gtatttccgc cgccaacttg ggcttgatca aaaacggtca aaaaaacatg
    42181 ctttccgtta tcaagatcaa cttcaatttc tgatacacca tttacaccat ataaaaattg
    42241 tccagcttga taaggcgtat taatattggt aataattttt ttcacttgat tgaactctgt
    42301 tgcagataac ggttcatcta gccgagcttg attattttgt tctagaattt ttttgaagtt
    42361 gttccataat tgttctgttg ttttaatttc agctttatat tcccattgtt ttacgccacc
    42421 gattcttgtt agataatcca caacttcttg ctcgaacctt aattcagatt gactttttat
    42481 accgaattgc tttgtcataa tctattcctc caaacttttt tctaacatag aatttgtaaa
    42541 ttgctctaac aagcgagtat atatacttaa cttactttgt aatttcaatg attcagtgta
    42601 aatcccccct atttttcttt gttcttctat aagaggtagc ggaataacaa gttgttccag
    42661 agactgtttt gttaacctta aaactggacc tgttccttga agacctcgat ctttttgacg
    42721 ttgaatatct ttatgattgt taaataagta aagaaaatag cgcttgtcta gcaaatcact
    42781 tttaaactct acttttgtaa aatttagaga cagaacttta ccaacattct gtccacttac
    42841 cattgttgcc cgttgaagtg aattactaat aacaacatcg cgtttatcta aagatacttc
    42901 ttgatcaaat aaatagttta ctctttcctc ttgagtcgct tcattataat acttatgatc
    42961 ttcttcaaat gaagcttgat cgtaataatc aatttcttga ttgccaaact gtttttcagc
    43021 tcttgattga ttaattcctt gaacaaaggt tacaaactca gaaagctttc tcatttccat
    43081 atatacttcc tcctaaaata caattcaggt ctcatttaaa cgatttatcg aaataatcat
    43141 atatttcttt ttcgtttctg cgtttttata ataaacctta tacagtaatt cgtcaatgga
    43201 aataatttta tattacaaaa aatccgcaaa aaatgcggat tcctttaaat atttaatctt
    43261 tattctagtg tagtacaagc ctatacccta acgttacata tctcattcat tattatacca
    43321 gtatttcttc atgacacata agggtgtaac ataataattt taaacgaaat gactacaagt
    43381 agtcattgaa gtaagtcttc ataagatacc tgtaggatat ccctaattgc tctcaattga
    43441 tctaacttaa tgtgttgccg gccaccttca attttaacca atgtttccct agttatagca
    43501 attttatgaa gctgtaggtc tctaatcagc tcggtttgac caattccttt ttctaatcgg
    43561 attcgtctaa tattgctacc gattacattt tctttatcaa taattttttc ctctttcaag
    43621 aatagcacac cttttggact aaaattagtc ctttttctta ttaatcttaa agattcggta
    43681 tgctacaatg ggactagtat tagtccacat tcaattttaa ggaggaattt ttttgaagaa
    43741 atttgcaatc attctaacct gcgctttact catcggaact ataagcgggt gtaccacttc
    43801 tgaaacaagt tctacaagta cggaaactag tacaacaacc tcagaaaaga attacccaat
    43861 acattttttt ccggttccag atggatttga agcttattat catgataaaa aactagatgt
    43921 ccctttatca gaaactgaat ctactgaaaa gattttgcca attgaaattg aaagaaatca
    43981 agacgatata aaactaaaag ctgatcttcc cttactgaac agcacacaaa ttgatagaca
    44041 tgaaaatatt aaagttaata ttgaaaaagg agcgttagca aaactttata ctgaaaaagt
    44101 tgaacaattt tttaagtctg gtgccaataa agacttttca aaaataagcg attattcgga
    44161 gaactttttt aatgaaaccc aagatgaaat catgaactca acttttatgg ctgaagatat
    44221 gtacaatatg acattaaaaa aaataaaagc ttacgaccat ccgttaaatc tgacactaaa
    44281 taatggactg ctttcttttg tattagcagg ttacgttgac tatgaatatt caccagatta
    44341 tttggagaaa cctcttcaac acgagaacaa tgatattaat tttagatttg tctacgagtc
    44401 aaaaagtaaa acatggttag ttgatcaatt aagcacgata aacacaagct tccatgaatt
    44461 acctaaacct gaaaatgaag ttgagatggt cattaaaatc ttctaaaaaa gaagcgttga
    44521 aggagaaata ttcctttcaa cgctcttttt tatatgctta gaaacataaa taaattgtat
    44581 actttctttt tagcccctat tacttttcac gaaaaatatc tttttttcga cgctagcgtt
    44641 tctctacttc taatactgta cacagttttt gtattattga aaaattactc attatttagc
    44701 attaatccac atcgttttat caatcgacct tcatactaaa ctactttaaa gtcatcttcc
    44761 ttctcgtttt tcattaaatc agatacataa gcccatgtac ccgttcgttg taaataaagt
    44821 tcttcttgta acaactccca ttttctctta gaaatcataa tgacgccatc gttcaatcca
    44881 tttttagaat ccctagtaat ttctatttca gaatttgaat gaactacttc ttgcaacact
    44941 tggtgttagt atcaaatctt agacagcttt tcgtagagac aagaataaat taaacttatc
    45001 tacgagagga tgaattttta tgacccgata tgaagaaaat tttaaacaaa tgattgttga
    45061 actgaatcaa actggacgtt ctgttcgagg gttagcgaaa gaatatggct tatctgaagc
    45121 aacgatttac aaatggaaga atttatattt acctgatcag tccacaggac tgactggaaa
    45181 agaagtagct gaactgagaa aagaaaatgc tcgtttaaat gaggaacttg aaatcttaaa
    45241 aaaagccgca gccatattct ctcggaaaac ctaaattcgc ttgttcaatt tattgaaaaa
    45301 tggtgtaagg attacactgt ttctttgttg tgtcggttat tagaaatccc tagaagtgtc
    45361 tattattttt ataagaataa accgttgaca gctacagaaa tcagaaataa tacgttgaaa
    45421 aagaagattt caacaatttt ttttacgaat aaacaacgct atggtgccac aaagatccat
    45481 caagttttat taaaagaagg gatttcagta tctcttaaac atgtccaaaa gctaatgaaa
    45541 caattaaatt taaggtcgat tgtagttaaa aaatatagac ctcaaaggtc taataaaccg
    45601 atcatttcaa aagagaatct cttaaatcag gacttttcta ctgaaacgat ctgtgagaaa
    45661 tgggcagctg acattacgta cattcctact aagaaaaatg gttggtgtta cttatcttca
    45721 atcatggatt tacacacgaa aaaaattatt agttatacat tttcaaaacg aatgactgtg
    45781 gattgtgtca ttcaaacatt gaataaagca aaaatacatt atcacattcc agaaggaatg
    45841 attctacaca ctgacttggg cagccaatac acagcaagag aagtggaaca atggcttaaa
    45901 accaacaaaa taaggcattc ttatagtaga aaaggaacac cttatgataa tgctggaatc
    45961 gaatctttcc atgcctcatt gaaaaaagaa gaagtctaca cgactagcta ctcagatttt
    46021 gaagaagcaa atcgagcgct atttagctac attgaaggat tttataaccg aaatcgaatc
    46081 catagctcga ttcactatct aaccccacag gaatttgaag aattagcaaa agaaaaaatg
    46141 gcttaaccgt ctctactaaa atgtgtccaa gatattgact caaatccaac tttacctata
    46201 ttttctttga agtttgttgg agtaatagta tccaaaatga acacctctct tctctatacc
    46261 tctattatga agcgcccatc aaataaagca agcgttgagg aataattcta tcaacacttg
    46321 ctttattaag ctttcatttt gtagtttgac taaaaccaat aatcttattt ctaaactctc
    46381 gaaaaacttc ttgtggctga taatagtctg catctatttc aaatgtttct gagaataatt
    46441 cattgaattt ttctactgaa ataatgattt taccattctt ctctcgactg ttttctatta
    46501 tttctgcttc taattcaaat aaatctagtg acgaattaaa atcataaatc ctgtcatatt
    46561 gaacaaaaaa atcaagaaaa agttgctcaa actctttctt atttatcact agtgtttcaa
    46621 gcagatcaac tttattttct tcagtcattc ttccaatttc ctcactttcg ttttcaaaaa
    46681 tcacaaaaat gatattaact taaaagccac taacaaaata agacttacaa attaaagggt
    46741 aatccaacaa aataaccatt cgctctacgt actacgattt ggtagtcctg cctttcaatc
    46801 ggtaattcat aatagtaatt tactgtttct gatttaccac tttcaatata ctcaggatta
    46861 tccaatatca tagcatactc ataaagctct tcaccatcct tattttcaat cgaccattgt
    46921 tgcggacgta tcggattttc tgtatcattt ttcattgtat aggtcacttg taatacacga
    46981 tcatgctgtg caaattcatt tcttttttcg gtcagttcaa tatttttaaa tgttagttca
    47041 accccatctg cctgataagt ttcacctatt tttaaatctt ttgttacaat ttttattggc
    47101 ttttcttctg gaaaatacct ggctttaatt gttagataac ttccaaaaag aatcaatcct
    47161 agtagcacaa aaaaaattcc tttaacgagc cactgaaacg ggaagtaaac tttaaacatc
    47221 ttttttctcc tccgttttgt atttggtttt tagagaacac gataactttt gatcaatacc
    47281 atccaacttt ttcattatac ttgattctag ttctgaaatt gtttgttcaa gtttaatgct
    47341 ttcaggatca ctaaattttt tatttgattg actagccaac attggtaaat ttgaaaccat
    47401 cacacggaac atatttgctt tcgttccaag agttccttta ttcttctcat aaaagctgtc
    47461 aaacaatttt tttgtttcct ttgaacaata tactttaata tacggatcag acattctttt
    47521 ccctctccct gtttacttaa aaatccatat catattgatt ttgttgtttt tgataatcaa
    47581 ttcctcttgc tgtttcttca tactctctcc gagctttttc aatcgtacca tcatcaattg
    47641 cacgattaaa taaataaaga acacgttttg tttcttgaat ataacgcttc ttaaaattac
    47701 gttttaaagc gggttcagta tcttgaatgt taaattggtc ataatcagtt gataaactct
    47761 ctgcgatatc ataaacaact tttttataca ccaaatttac acatttcttc ttatatttta
    47821 aaggttcctc ttccactgca aatccaaaga attggttttt attctcacca aaaacatcca
    47881 ataaattttc tattctgtcc tctatttcaa atttatattt atcataaaat tcttcataat
    47941 cttttgatga tctaagaaat ggtatattct cattatctaa ttcaaagcgt tgcgctcgtt
    48001 cttgccaatt aaatctacta tccgctctac catttaacga tttaccaaga tatcgaatag
    48061 caatttcagt gagtacctct tcagattgaa tttgctccgc ttttttattt tctgtgaaca
    48121 aatcatcaag ataattcata tcatagtttt gccctaaacg ctttccgcca atctttaaga
    48181 ttaacccatc ttcagaatct aagagtttgt acccaatacg ctgatttttg gcaaaccggt
    48241 aagtctcaat agaatggttt ttcttcaatt ccgcatgaaa atcatcataa tttttcgcat
    48301 actgagcaac ttcagaaaca gctagtttta ttttttctcg atttgaaatc ttattttcag
    48361 ttctaaattc acgttctttc caattttcac gatctgcaac taaatgatta tacttataca
    48421 tatcaagtgg gttaattcca ttatcaataa gtatattatc attccaagcc tgcaaaactc
    48481 gaatatcatt ctttcgaata tccattttct ttcctgttcg ctgatcgttt ggcgaaatac
    48541 caaagtgatt gtgaacatgc cctttgtctg tgtgtgtgac aaccaaaatt gactggttag
    48601 gaaacatttt ttctgcccac ttaacaccca actcatgtgc tttttcagcg gaaatatctt
    48661 ctagtggatc aaagctttgt gtaaaatgcc aaagctttat tttcccttta tcaccaaaaa
    48721 tttcattggt tcgtttaaat tcctcaatag catttaaatc attcgatacg ccaattcctg
    48781 tcacgagttc agaatcttct tgtttattta acatatatga aattgtatta tgaatactcc
    48841 tactttttga agcaattttt ataatcgagc ccataactta ttcaactctt tttccaagcg
    48901 attcaatgga tcacttattg attttatttg tatcattaag tcattttttt cagcatatag
    48961 ccgctttaat cgctcttgct tagttgtaaa agataagtaa acactctgat gttcttcttt
    49021 ttgtatctcg gtatcttttt tatttaactg aacacgcaaa taatttgctg ttttcactaa
    49081 ttgattcaaa tttcttccta ttgctagtaa atgaaaatta atttctttat attgtcgatc
    49141 ggtatatttt agacttttcc ctgcgttgtc tactgcgatt ttcttcacgt acattggaac
    49201 agataaacca aaaatgtctg ctgttttttc tagctcttgg tactgataat cgtctactcg
    49261 aaactttatc ggcctatttt gtcgtcgact atcttcatta cgtttatatc gctttctcct
    49321 ttcttctaat ggtagtttac tgacatcaat tgccatgcgc acttctcctt tgcaatatta
    49381 tttttaagtt caaaagaagc aagcgagggg aaagggaacc cttccccgct tgtcgaggga
    49441 gtaaactccc tacgagcacc ctcatgctcc gcattttatc gagattagat agccgataaa
    49501 tcagctacct aacactttct tcttgcgcct cttctcgata gaaaattcga tacgctgtct
    49561 ctccttttct tctcaaaaat tgttggaatt tcgtttccat caattgatcg cttgcaagaa
    49621 attcgtgtac tagggtaaat tcgctattcg cgaaatttac cgccttttgc catttttctt
    49681 gagtaccttt ttgattaatt tcaaaagttt cttcaaactc ttttactgat gaaattcctg
    49741 ttttttcata gacatacttt cccagttcgg gataaagttt tctctccaac tcggcgagtt
    49801 cttttttcaa atctgcaatt gacttttgtt ttttccttgc catttttcat gctccttttt
    49861 gtatgtgact aagttcattc ataagctcat ttttcatcac taattcttct tgaatcatca
    49921 gttcaatata atttgtgacc gtgatttttt catttgtcgc tttctttact gagtcgcata
    49981 attcggcaag ttctaagaaa tcctgcgtac taatctgtag aattaattgc atgacaatca
    50041 cctctttatt ttttaaatag actatcaaac agcggatttg gtttatcaga aatatcctga
    50101 aacagtggat tattttcata agtaaatcgt tgacgtttct tctccttcac ttcttgttct
    50161 actatcactt tttcattagg tctttctttt cgttcctcga caatatttat tttttgcgtc
    50221 ttatctccct gacttgcttc agctcttgtt tcacgtactt ctaccgttct tggttgattt
    50281 gacgcactac cttgtacatt ttctcgtaca tctactgttc gatttccgct ttgactactt
    50341 gtagtattct gcaagttttc tcgtacatct atcgttctcg gttgatttga cgcactactt
    50401 tgtacatttt ctcgtacatc tactgttcga tttccatttt gactacttgt agtattctgc
    50461 aagttttctc gaatatctac cgttcttggt tgacttgtcc cactactttg tacattttct
    50521 cgtacatcta ctgttcgatt tccattttga ctacttgtag tattttgcaa gttttctcga
    50581 acatctaccg ttctcggttg gcttgtccca ctactttgta cattttctcg tacatctact
    50641 gttcgatttc cattttgact acttgtagta ttttgtaagt tttctcgaac atctactgag
    50701 cgcctatctt gactacttgt agaattttgt ggattttctt tgactgattt ttctaatccg
    50761 gtatttgaat ttttagcttc ttggattctt tctttagtca acgcttcttt ttcgtccata
    50821 ttagcgcctg tacgcccttc tacacgtttc cttaacattt cctcacgatt cattgcttca
    50881 ggcgtgtatt ttgcttcctg tcgcttctga ttaattaatt cttcagcaga cttagcttga
    50941 ccgtgcatag catttttacg ttcttcaata tcttgtttaa ctttttgctg aattgcactt
    51001 gcaggattaa tatttgattc agcattattt ttcattgctt cacgcatacg ttcagaacta
    51061 gacataattc tatcagcgtt ttcaacttta gcgccatctt ttctatccga aagacgtgtt
    51121 ggcatagaat cagcatattt tccagtttca tcttctttat ttttagctac tataggtttg
    51181 ctatttttat taatagcgga catggtacca tcatctaaac cttctttgaa tttattcttg
    51241 gtatcattag ccatatcacg catactctta aatggttttg ttgctctttc actagccatt
    51301 gtagctccat ccgaagctaa gcctgataat ctacgttcac gagcataccc aagcgttttc
    51361 cccgttttat tagctttttg agaaagtgat tgaccaaagt tgcgttcagg ttttcgttct
    51421 tctccttccg cttgatcgtt tccaccacta tttttcccac ctttagatcg gttcatacca
    51481 cctgttcctt tacgaaacag cattgcaccc atgccaaaag cagaagcaag ttgcccatag
    51541 ccttctttga gtccaatatc cacaccaaag taacgcatga ctgttccact gcctttgatt
    51601 aaaacaaaca cagcacagat ataagcaatt gtttttataa agatatttgt tgagatacct
    51661 tcaccagcat ttagataatt gacaaacatt gcaaacatag acaatccgaa cccttgaaat
    51721 ccgactgtca aataacactg taaaatatct gacaaaactt gttttgatcg ttgacctgtc
    51781 tctaagtcag tagcaaatac caaaactcca agtacacgtt taaatgctaa ctcgagtatt
    51841 gctgtaatta taatgaaagc actaaaaaca tacgctactg ctaaagcaat caatccaaat
    51901 gaaattcccc acaaattcgc ttgatagcga taatatcctg atttgatacc atcaacaaag
    51961 gttgacaaaa aattatctga aaatttagta gcaacaaatt cattatcaga attttcaact
    52021 aattcataac gcaggttatc agctgttggg ttctcttttt ccattttatc aattacatta
    52081 tctgtcaata cttgcgtaag atccgttttc caaaaatttt cttttgttag attattccgg
    52141 tcattcgttt tgttaatcat tgaatactga tctgttttat tgatataagc taaatcagtg
    52201 accccttgtt caatcagcga ccacgccacg ccttcgtccg aatttgtaat cgtagtcgta
    52261 tcattataaa atgttttaga aaactctatt ccactgtgaa tcaaagtcgg cattaaaaga
    52321 atcaatgcta ctgacattac aacattcatt ccaactgatt ttagatcaac cgttccttta
    52381 cctgtaatca ttttataacc aataatcact agtgaaagta tcattaagcc aacaacaatg
    52441 gtacttaaaa ctgattgata gactttattc aatccggtac tttctacaaa gctaaacaaa
    52501 tctaaaacct cgggaatcat attttgaata ccatcgacaa tagcgaacat tgattttata
    52561 acagaccagc ctatccacat accgaactgg ctgtaaaaat ttccgttata ttttttaaga
    52621 taattctgat aaagtggatt agttaaaaac tcagtttgac tctcagtatc atcaaaccag
    52681 ttgaaaatac tgccttctgc aacgttactc gtaaaaataa tcatagctat gagaataaga
    52741 aaactaatca acaaccaatt tatttttcta ttatcaatct tttttttaat tattttcatt
    52801 gttacctccc tcagcccatt tttttaggct ctcagaaagc ttctgagcca tttttttatt
    52861 aacgatgatg tgagttacgt aatcatcatt aacagctctt aaatctagaa caatagtatt
    52921 tgagcagata ctctcgatta actcaagagt atctgcgtta tgatcaagac agtaaatctt
    52981 atctactaga tgattttcaa tcaaaataac ccctcccgta gtcaaagttc tcaattttat
    53041 gaagatatcc ctctacttca tttaaatcaa ttctattctt attgctgact aacccgttgt
    53101 aaaattcttt ttgtagatta tatttatctg tttcttgaat ctttttatat aaatcaacca
    53161 gggagtccct gtctttaaaa acttgcttga attgttcgct tgtatatcgg ttttcattaa
    53221 ttgcaacaac tgcttgaatt tcaggccatt tttctttcaa cttaaaataa cggggaacgc
    53281 tgaaatagtt ctcaaaaaag aagtctcgtg cttcatagta ttctctattt tgtcttttta
    53341 tatcctcgac aatttcgtat cgtgtagaga tcttcaaagc tgaactttcg ttaatttctg
    53401 ttccttttcg aaagactttc acttttgaag ggcgttcaat tttataattt atcccctttt
    53461 tacttgaata acttttgata cgaccatcaa attgaaccat atctcctttt tttaaccacc
    53521 caaattttag aaattgttta gttaaattca tccaaatgtg gtctgtgacc aactctaagt
    53581 tatccccttg aacaattttt aaattcgata acataaaagt agcattttca tactcttgac
    53641 caaacttgta tttatccttt ttatatccgt taaattcaaa ggtaccttga aatgtatgcc
    53701 gtttcttatc tcctaatttt ttcaattttt ctctcaaaat aatcaactcc atcatttaat
    53761 atatttttgt gatggtagat actctcgatt aacacaagag tatctgcgtt gtaatcaaga
    53821 caataaattt gatatattaa ccgtttttca atcatattat taatcctttc taacctattt
    53881 ttttcagtgt ctctagcata tccagtctac tagctttttc ttgcttagct atgtcctccc
    53941 agtggttcct agctgtggtg ttgataaact tcatttcatc aatttcttca ccgagattca
    54001 gtttttcatt tatagaatta aaaagtcttg ttagcatttt gtttgttata tcaaaatagt
    54061 cttgacctcg aatttctatt ttatattctc tttcaattga atctttcata ctagcaatca
    54121 acttaagata cttttctctt tgaacttcag tatatctatc acctagtctg gtaattattt
    54181 gaattccttt ctgatttata atattttcaa aaaaggaatt ctgatcttga ttaatctctt
    54241 ctatactatc cttatcttga ttagtttgat cttgactatt ctgatcttga cttaactgtg
    54301 tatccgattt gacgtcgcat ggtataccag ctggatacca tttttccatc agatcaactc
    54361 ttaatggttc tacttgacca gactgcaaaa tcatatcaaa ttttattggc tttagctttg
    54421 agaaaaattc tggttttaag ttcaataact tgaatcgata aatagaaggt ttatacctat
    54481 cgattcgtat attttgattt tgtaaccagt ggggtagaaa cgctaacaaa tcttcattta
    54541 aaaaaaatac aaaacctttt gtaaaaagta tctttacatc atcatcgggt gttccgagca
    54601 ttttcgtgta tggataagct tcgacaattc cgtcatcatc tgcgtttgtg ttcagatata
    54661 cgtataataa ttttgctgaa tctgacatct tcataaaact tgcattattt aaaaatgatt
    54721 tagccagcat tcgtttttct gccataaaaa tcacacttct tcctatttga atgttaaaat
    54781 gtagtcaaat aaagtttagc tagaacttta ttattgagcg ttcctagtgg tctgcaaact
    54841 aacgggaaca ctctttttta ttcttattaa aaccagcaat actagttcac cttctttcat
    54901 ccttttacat ccaattaaat ttgatgctgt agcatccatt tttttacctc ttcttttgaa
    54961 tatagttcaa ttgtgtcaac cacgataata ggtaatccta accttttaaa cttcagaaaa
    55021 gttccatatg atacgtttag aaattttgcc atttcttttc ccttaagaaa aggtcgttct
    55081 aatccgctgt ctattcttgc ttgatcaata gcttcttttg tcacttcaaa cacatgtgtc
    55141 ttcaactgat ttgtttgatc agtattcaac attacactga ctccgatttg ctccattaaa
    55201 aattcccact ttcatatttt catcaataag ccaactcgcc aagttgtttg ttaataatct
    55261 gatttcaata accaacctat tattttttga taaacatttt tcgacacact ttgaacttcg
    55321 aagcattcaa tttttttaag agtttgcctt gaaattccaa ttaattctgc tgctttagtt
    55381 ttcgtaatca tcaactttcc tcgtctctct cgaattctta tactaaaatc ctcattaatt
    55441 tgaaacataa acatccctcc atttttattt ttgcatttat ccgtttatcc gatgtcggat
    55501 aaatagattt tagtatatat tattttgata gtcaattaaa aatatacatt ttggataaaa
    55561 ttgttcattt ttttatccaa atcatatatt atagatagta agaggtgaac ttcattgact
    55621 caaaaaaaca gaattaaaga aactcgcgaa gaacagaatt taacacttaa agaacttagc
    55681 aatctgtcgg atattccgta tcaaactttg agaaactatg agattggtaa aagaaaaccc
    55741 agaaatgatg aagtttggtg gaagttaggt acgattttaa acgtccgtgg gccctactta
    55801 aaaggagaag atagttttaa aacaaatgat gaattgcata tttattatct aaaaaaaagt
    55861 catacaaatt ttgaactgtc taagaaagaa gaaattatag atataataga taaactaaac
    55921 gaagacggtt tagatgaagc cttacggcag ttagcactat tatcaaaaat agatgatttc
    55981 aagcggtaac ttacttatca tcatgtagac tatcaggtat cataaactaa ttcctcaatc
    56041 agccaactcg ccaagttatc agattggaga gattagttat gtcaatacaa aagtacaaaa
    56101 caaaaagcgg tacacgatat agtgtccgct tatatgttcg taaagatgaa aatgggaagc
    56161 aagaatacta tgtaaaaaaa ggcttcaaaa ctgaaaaaga agccaagtta cacgaagcaa
    56221 gaaaaaaggt cgagattgaa gatatgggct atcacagacc ttctaatgac ttatttagaa
    56281 cagtctatga agaatggttg ccgtcttata aaaatcgtgt acaagaaacg acttatcaac
    56341 gaacaaaaga cctttttcgc ttgcatatcc tgccaaagtt cggagacaaa tcgatttcta
    56401 agattactcc tatttactgt cagaatgcaa tcaatgaatg ggctgtaaaa tatacaaata
    56461 tcaagcaact taaatcctac agctctcaaa tatttgagta tgcaatcttc gcagaattgt
    56521 tacagcgtaa ccctatgaca aataccaaac tgccaaaaca agaaaagaaa gaatccgaaa
    56581 attatttctc actagaagaa ttaaaagagt ttttggaaat tctcaataaa gaagagtctt
    56641 taaaacatat cctgatcttt caacttttaa ttactaccgg tgttcgcaaa ggtgaattat
    56701 cagccctacg ctgggcagat attaattttg aagaacagtt actacacatc ggaaaaagtt
    56761 atgcaacaat tcgcactgaa gatccgaatg ctactagaaa aacaatcagg attcagaaga
    56821 atacaaaaaa tgtctcttcc gaacgaattt taccgattga tactcaaacc atcgaaatgc
    56881 tgaaactttg gaaaaaagag caagcagagg tacttttaca attcggcatt aatgcaaata
    56941 ccaaaaagca acttatcttt acctatgtaa atgcaaatgg cgatgttaac caacctttac
    57001 acgcagatta ttccaataat attatgagac gtttagagaa gaaatataaa tttaagcacg
    57061 ttactattca tggattgcga cacactcacg ccactttact tttagaaggc ggtgcgagca
    57121 ttaaagaagc acaagatcga ttgggtcata aaaatgctga aactacgctc aatacttatt
    57181 cccatgtaac tgaaaaagcg caacgaaatg cggtagacaa gtttattgct tacactggat
    57241 tatagacaaa aaagttgccg tcattacctt tttcattacc acaacgaaaa aaagcacctt
    57301 ccaactgaat ggaaagtgct tgaaatcgtt ggtataacaa cgattaacgc ttagagaatt
    57361 gtgaagcttt acgagctttc ttaagacctg gttttttacg ttcgaccata cgtgcgtcac
    57421 gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact tctagcaatg
    57481 cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg ccgtttacgt
    57541 taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga ttgattactt
    57601 cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca gtaattttgc
    57661 cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct gtgccgatat
    57721 attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg tctaaaactt
    57781 ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat tttgtaaatt
    57841 gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg atcaaacgac
    57901 gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct gggtagttgc
    57961 tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact ttgtctgcat
    58021 tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt ttatttttac
    58081 cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca gttgcgtcta
    58141 ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta cgcacgttat
    58201 tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg gctctttgtg
    58261 gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa cgtttttctg
    58321 cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat aaaagggtcg
    58381 tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca caacccctta
    58441 ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat atccataaaa
    58501 cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg caggcttaca
    58561 acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
    58621 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatttcac
    58681 aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg gcgctttgcc
    58741 caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt cgataaagat
    58801 atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt ggtaaagatc
    58861 tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag tacgatccca
    58921 gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg tagaataagg
    58981 tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt tcaaatcatc
    59041 catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg
    59101 tgtttggtga acaacaacag acatttggct tagatctgca actaattgat ttaatacttc
    59161 tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac ttaaaataat
    59221 agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt ttcagttcgc
    59281 ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg cagttgagca
    59341 aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg atagattttc
    59401 tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc tatcaattcc
    59461 tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat cacagctcct
    59521 aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac atcagccatc
    59581 atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc tgtgagctga
    59641 ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc ttctagaatc
    59701 gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact gccaaacgca
    59761 tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat cagaccattg
    59821 gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt ttctcctttt
    59881 tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca
    59941 gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac ataacaaaac
    60001 gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt
    60061 cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt agaaagcggc
    60121 ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc tgctgctgtt
    60181 tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt
    60241 cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat atcaaaatat
    60301 ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct ataaaagata
    60361 tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg gctgttttgc
    60421 agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa
    60481 aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc tcggttatct
    60541 gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat caggccatgc
    60601 atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg tttaattact
    60661 tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc tgccatccct
    60721 tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct
    60781 gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga aacgttcatt
    60841 ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt atcttggttt
    60901 tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat cggatcattt
    60961 ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc tagatctcat
    61021 tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct ctcgatcatc
    61081 gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta tttgttggca
    61141 gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc aagaaatttt
    61201 tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt attttacttc
    61261 atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt ttggttgcag
    61321 gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt
    61381 cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc ttggagtggt
    61441 ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc
    61501 gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct
    61561 tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa ggttgtgaca
    61621 aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg tgacttggag
    61681 ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga ccctcagtat
    61741 tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt agctttttcg
    61801 gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct
    61861 cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta atcttctgca
    61921 ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg
    61981 tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc attatagtag
    62041 atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg
    62101 gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg atcatattcg
    62161 atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata agttggattt
    62221 ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg ctcttcaaat
    62281 ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt catataaaat
    62341 cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt gcttgtgaca
    62401 gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa aacgaacgta
    62461 cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga tttatgtttt
    62521 cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca gcttctgaat
    62581 cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct ttgagaaaaa
    62641 cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca tcaaaataac
    62701 ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat cccaatccga
    62761 tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc aaaacggatg
    62821 tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca gcaaaggctg
    62881 atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat cgctctagtg
    62941 actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat ggaatggaca
    63001 gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct acaagaacgc
    63061 catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa aatgaacgac
    63121 ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa
    63181 agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc
    63241 tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag gaacgccctc
    63301 tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc catctagacg
    63361 tccttcgcct acgattacga ggtctgcttg ctgtacccgt ttatcgaact ccagtaaatc
    63421 caatacgtag tcgatgccag agactatttt ccctgaagca aatgctaaca gtcctgcacc
    63481 cattccacca ccagcactac tgcccgatgc ctcgattact tgaggggcaa atctcatata
    63541 aaaatcatgc atggcttggt cagccactgc taattcattt tctttcaagc ctttttgtgg
    63601 tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga ttagtaacat cagtaatgac
    63661 agtgattttg gctttaatca gctcaatagg cacgttcgtt ttatcaactg tagcaatttt
    63721 acctagattt tctcccagtg cttctacttt ttgattatct tgatcgtaaa aagcataacc
    63781 tagaccttct gccatgccga tgccgccatc gttggtacta cttccgccaa cgccgatgat
    63841 gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga
    63901 taacctaagt ggctgttgtt ggtttagtgg gacatctttc aatccgacga tttctgccat
    63961 ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt tctttgtttt tgccaaatgc
    64021 attagtaacg actgctgttt gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt
    64081 tccttctcct ccatcgccta taggtagcag atcatattca gcctgaacaa aaacttcttg
    64141 aaaacctgat tgaatggctt gtgctgcctc taaggctgtc agactttctt tgaaagaatc
    64201 tggcgcaatc agaatacgca ttattttccc ctccgttcca agatcagttc tttttccagc
    64261 tggtattggt ctccattgac aaaaataggt aatgtttctt tcgtctcttt ttttattact
    64321 atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa
    64381 attttttccc atgctgaagg aagtcttgga gaaatagaaa gagttccatt cgttactgta
    64441 atacctgaaa aaccaaatat aactgctagc catagtgctc ctaatgaagc agcatgaatt
    64501 ccgtcatctg atgaatgcgg atctggtcct aaatcaatca ggcacgcttc ttggaataaa
    64561 tgatacgccc attccatttc ttggatacgg caagcctcta tggcgtgaat cgctttactc
    64621 aacgatgagt cgtggattgt tttgttttca tagtaatgca aattcttaga aacgattggt
    64681 tgagaaaaat actcaggtaa taagtataac agcatgacaa gatctgcttg cttgctgact
    64741 tggagttcat ttacttcgct acgggcataa tcaagtaaaa tagactggct gccaggacgt
    64801 tctttgtaag tggtcaaatc aataactggc ttactgagat acgtgtcatc ttgcggaatc
    64861 agctgttctt tgttaggcac tggcaaatat aacttatcta aaaaattttg acattctttc
    64921 aaaaaaagcg gatcgccagc ttgatattta acagctaagg ccacattata atgcgccaga
    64981 tagttcgtat atgcattatt gtcgatatat tctgtgtatt catctggacc aatgactctg
    65041 tgtatctcta aatgatcatc gacttttaca gctctgctca gccaaaaagc tgctgtttca
    65101 tgaatgagcg tttttccctt tgtttctaaa aacgattgat cctctgttgc ttggacatac
    65161 tcttgaacgg cataagcaag gtctgcactg atatgatgtt ctgcggcagc tgaagcaact
    65221 tcttgtcttg ttccggtttt gatattcaat gcagcaaatt tgggtgtctc ttcctgacca
    65281 gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt gataatctct tgctttttgc
    65341 cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat tctttgctac ttcaggaaac
    65401 tgatataaaa agaaaggcag gataaaaatt tccgtatccc agaaaacatg ccctttataa
    65461 ccttcgccag ttagtccctt cgcccctaca ctaacacttg gatcattgcg gggagtcata
    65521 atctgtaaat ggtaagcagc gaagtccatc gctaactgat cgaaagaaga atcgccttgg
    65581 atcgttatcc gatgctgatg ccaaaaatct gtccaataag acatcgactg ctctaaaatt
    65641 tctgtataac taacgctttt ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc
    65701 tgaagagagg tagagagcac gatgatcttc tccagcgtaa aggacgtatt ttttctgatc
    65761 ttttctgaaa aggaagcggt gatccggcga ttttttgctt ggaatacacc tgctttattg
    65821 aatttagctg caatcccaat atcaatttta ctttctgttg tctggtagga tgcactgatc
    65881 aaatcgtcac caaatactcg cagttctttt tcaaccaact gctgtgtacc aaaattggtt
    65941 tgctgtccat cgatacccgt tttcacatct acttggcagt cttcggtcaa cggcttgatg
    66001 accactttca gggctgcctg atggagatta ttttgatcag caacacgaca agtaatgact
    66061 tccacttttt ttccttgagc agtttcaatg agcgcatagc gtttaagctc tcccatttct
    66121 aaatctagtg cccgctcgta tgcaagaact ttcatcgtct caagagaaaa atgttcaccg
    66181 tcgattttta tttcccatct gatgacatct gggagattca ctaactcggc tgattctgct
    66241 cctaaaggtc ggttatatac accagctgca aacattcccc ttacttgatt ccgatagtct
    66301 tcctcctggc ttcctctgat accaaaaaat ccatttccaa tggtcatctg actcgcatac
    66361 ttattgatat actctaaatc aaactgtttt tcttctactt tcataaggta ataacttctc
    66421 ctgtttctgc cgaacgatat aaattttcca ccagattctg gatgacatat ccttgccagc
    66481 catctgcaat catctgagtc tggtcaccga gcgcccgatt cacaaaatga gccatactta
    66541 gttcatgtgg gtcattaact ggcgtggtct tttgtaagga aaccagcttt ccatctacat
    66601 cttcgaatag ttcgcctgga tacagattag cacctgcctg atcgccacaa aaagccacat
    66661 tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt cagttggatg atcttgccgt
    66721 tttctaattc cagtgtgcca aaaattgcat cttctactgt gaatttgctt ggatcccatt
    66781 tcccgaattt ccctgaattt tttttcgtac caattttttg gaagctgtac gcccgaacgc
    66841 tttttacttt aggaaatcca gtaatataca tcgctgtatc aagcatgtga atgccataat
    66901 cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa cgttccccag cctggaacac
    66961 cagaccgacg aagcgcattc gcttctacgt aataaatatc cccgagaaca tcaagatgat
    67021 tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa tccataagcc aaaatctgcc
    67081 cgtttttatc ggctgtctct gccatctttc ttgcttgatc agctgtcata gctggtggtt
    67141 tctcgcacat cactgaagca ttagcttcga gggcagccaa tacatgttcg aagtgaaagc
    67201 gattgggtgt acagacactc acgatatcgg gagaaatctc attcagcatt tgtgtaactg
    67261 atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg
    67321 gatcaaccac agcggccaat tctaagacat ctgatctttt ttgataataa gcagcatgta
    67381 ctttttctgc tacttgtcca gcgcctatga ttgctacttt cttcatttat ttctctctcc
    67441 tttgcttatt tacttattgt tattctttct ctattctttc tctaaatcat ttaaatgacg
    67501 cgctgtttcc agacagtttt tcaagtattg caatgattcc tgataaattt tcacttcatt
    67561 ttctcctcgc aaacgacatt catataccat ataaccttca tactcatttt gaagtagcgt
    67621 ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt tggtagcgat gattatctgc
    67681 taaatggata tgacccacat actctttttg cgtagtcaat gcttttgaaa tatcatcttc
    67741 ttcaatattc atatgataaa aatcagcgat gatctgcaca tgtttcaatt gattagagtc
    67801 aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg
    67861 ctccaaataa ataaccgtac ctgtttcttc ggctacttta tctaaataac gaagtgattc
    67921 gctgactgct ttatagtctc cttctttgct tcggggtgaa accatcggcg gtaatcgata
    67981 agtgaacatt ccccaagctg ctgggataac gattcctttg cctccaacct cgcttagtgc
    68041 acgcaaaatc tcagcaattt gacgtacacc attaagacgc cgttcttcga taaagtcacc
    68101 gatccaccca tcgtagccgt tacaagctgt cactactggg atccctgttt ctttgattgc
    68161 ctgtttgact gtgtcgatat tttctattaa caacttaccg tcgatttcgt attcttcaaa
    68221 tccacagtct ttgataaatc taaatttttc taaaatgtct cttgggaaaa atgcttgatc
    68281 ttgcgtaccg aatttcattg ttctcctcct cggacttgaa cgcccatttt gatactttct
    68341 tctgggtgct ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact
    68401 ggatcaatga tttcttcaca atccaagtaa ccgttcatca ataattccca acatgtgtcc
    68461 tcgattcgtt tacggttcca tcttgggtaa tctgggtttg gttcactaga tgctcgagca
    68521 aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa
    68581 ggtttagcaa aggcaacata agaaatgatc ccgccatagc cgatccctat taaagaagct
    68641 tgtaatgcgg cagcactgcc actcgtttca ataactgaat ctactccacg tttatctgtc
    68701 aattccttta gacgaaaacc gacatctgct ttcataggat ctagcgtgta atcggcaccg
    68761 tgtttttctg ctaattctct acgctgcgct atgggatcga tcccaacgat ggtagtagca
    68821 cctgcttttt tagctaattg aatcgcaatt tgcccgatag cgcctaaacc gaagattgct
    68881 acagcatcac ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa ttgagcagga
    68941 tcgtaacata ctgcattttt ccattgatcc aaccgatcaa gtttacgtag tttataatta
    69001 tcaacaccgt ttgtaatcag cgtttcacga atgggtccat aagtgcagac taactctcct
    69061 ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat catatttcct
    69121 aattgaaatt cgccaaatac cacaccgcgt tggcttcctt cttctcttgg caagaatagg
    69181 cgccattctt ctgaaaattc ttcatcgata aatggactta atcctctaaa gtcgacaatt
    69241 tctgttcctg gttttggagt agcgaaaatt actcggatac gtacttcgtt cttttcgatc
    69301 tcacggtctt cgtaatcttt gaatccggct attcttggtt ctaatgcaac taattttttc
    69361 atcttttctt tcctctttct tttcttttta tccttttacg ccgccttcga ctaaatcacc
    69421 tttaataaat cgttcagata acgcatacat aatgatgact ggtaaagcgg tcaccaatga
    69481 agcggccatc attcgtcccc aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat
    69541 tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat aatcattcca
    69601 cgaaaccata aagcaataaa cgaagacaga aatgattcca gaaatagcca atggtataat
    69661 gatccgaata atgatctgga tccggctaag accatcgatc attgctgctt cttcaatatc
    69721 agtcggaatc gtgtcaaaat aactttttag catgaatatc gcagtaggaa gtgtttgaac
    69781 gaccatcgtg ataatcagtg ctgtattggt atcatacaaa cctagaccgg aaatgatttt
    69841 aaataatgga acaacgatca aaataccgga aaacatataa acggtgtaaa agctagcatt
    69901 gattttcgag cgagctttga agcgtagttt tgacaaagcg tatgccccgc tgatcccaat
    69961 cactactgca gttacggagg ctatcaagga gatcactaaa ctatttttga aatacatgat
    70021 gaatggaaaa atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg
    70081 aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat
    70141 gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat
    70201 tttcctgctc tttttatttt tcatttcaag tccaccttct ttcgtgagag caagacgacc
    70261 gctgcaataa tgatgaacaa tagaatcgat atagctgctg cttttccaag atcattgaac
    70321 gcaaaggctg tctgatacaa ataaacgcct aagatattga ctttattcgt cagtagatag
    70381 acatctgtaa acatatagaa catccagata gatcgtaaaa caatcacagt tgctagtact
    70441 ggtttgattg ccggcagtgt cacgatcttg aacttctgcc aagcatttgc accgtccatt
    70501 tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac
    70561 gcatacggat agtatctcca gatagcaaac aaaacgacca taaagaagct agcaactggc
    70621 tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc tatctactaa cagatcattg
    70681 acgattccat aactattatt gaacatatat ttccaagcaa aaatcaaaga aatagatggc
    70741 gtaacataac ttaataaaat cagtgaacgc accgttttcc taaatctgaa cttgcggtta
    70801 agcaaaatag ctacggctaa tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa
    70861 gcaactgtta aacctaaaga acgataaaat tcactatcac ttaaaatttc aagataattg
    70921 cttaatccga taaaaacggc gtctgagtta ggattcagcg gcatatcgaa aaaactgatc
    70981 tgaatattgg aaagcattgg aatcagcact aaaacagcta ataaaagcat gcttggtgct
    71041 aaaagtccca tggctaattt taagtcagaa tgtttccttg gacgattcat tgaaacctcc
    71101 ccctgataat ctgccaatag ctatctactt ttttcgaaca ccattggcag aacttcttgt
    71161 tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt tccgacagtt
    71221 acttggttca ctgcttttcc gatcacacct gagcttgtga tatctcccat tttcgtgaaa
    71281 ttcttttccc cgactagtcc aaagacttgt acttcatcaa aagcactagc aatctcagat
    71341 ggcagctcgc caaaagactt gatgacttca ttttctttgt aaccatccag ttcgactact
    71401 tgtttattca ctggttgagc accaccagga gacatcaaga cccacttttc catgtttttc
    71461 ggttgagata agaaagctgt gaatgcttca gcagcctctt tttgtttctc gtctagacca
    71521 gaagaaatcg tcaatccaga tactgtgcca tagacagctt gatctttttg tgttgggata
    71581 gcaaaaccga tattttctga gttccccgct tcataaacag aaggcaaaat gtaagttgaa
    71641 tagattgcca ttggtgctgt tccattcatg aaagcatctt tgatttctgt cacatcatta
    71701 gagcctggca tcgtgtattg ggataagttt tgataaaacg acaatgcttc tctcatcttt
    71761 tccgtatcga tcgttacctc gccttcatca tccaaaacat ttgctccatt tgacaaagca
    71821 aattgagaga acgcttgctc actcattgtt ccttcagcag taggcatagc aattccatat
    71881 tgtttattct ctttatctgt gaaatgttga gcaattttta aaatatcatc ccaattttct
    71941 ggttctgaaa aaccagcttc tgataatttt tctttgtcat accaaatacc ttgaacccag
    72001 ccgctaattg gtgcagcaat atagcttttt ccatcttctg aacgaacaag gtttttggct
    72061 ccgtcataat aattatcttc tccagcttcc tcgataacgt tttggactgc cgtctgatca
    72121 atcagttcat ctttatccat cactttagca aaatcctgac tgacttccat tacttctggt
    72181 aatttaccgg aacgagctaa cgtaacgatt tttgtattga acgctgattc ttctaccggc
    72241 acttgcttga ttttgatgtc tggattttct ttttcaaaat cagcaaccag ttcattgatc
    72301 acacttaatc gttcctgttc gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat
    72361 tcgttagagc tttgactttc accacaagca gtaagaagaa atccagtagc tagcagacag
    72421 gtagtaacaa taattttttt catttttttc cacctcttat tttattattt tcagccacat
    72481 atattcatac ggctggaata tcaactttcg ttcttgactg caatggataa tttgtccaga
    72541 aaacaggtct aggtaatctc cttttggtaa aagataacct gctgattgat tggaaaaatt
    72601 atggataact aagactttct cttccttcgc ttcgcgaata aaactgataa aagaatctgc
    72661 tgagtccagt aagtaaatca aactattcgg atggaaggct ttttcttttt ttcgaataga
    72721 tagcatctgg gtcaattcac catatactgc tgttcttaat ggatctgtct gcaactcttt
    72781 ctcaatatct gctaacaaaa atttttgccg attgattttt cgattctctc ctgttttttc
    72841 tacgcctttg taatcatttc gacttcccag cacactttga atataaattg ccggcacgcc
    72901 tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt
    72961 atctgttgga gaacttaacg catctagata cgtagcgttt atctcatatg gactggtcgt
    73021 tccatctggg tttttcttgt agtttactcg ggcaccctct ttcttcagct gttccactaa
    73081 ttcaaggatc gttttttccg gcagaatacc tcgtaccgga ttcaatccga taccgtcatg
    73141 ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg tttttaacca
    73201 ttctttcaac ttacttgtat tacccgaacg aatcgcttga agggtcaaag gtggtagcga
    73261 aaactgatag accatctgtg cttcattctc gccattccca aaataactga tattatcttt
    73321 gtgtggcaca tttgtctcag tgatcaatac cgttcctgga gctgcttcat cagcgattgc
    73381 acggaataat tggatgattt tatgcgtttc tggcaggtgt atacaagaag ttcctagttc
    73441 tttccataaa aatcccacag cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa
    73501 caaaagaata tcgatcattt tcaataaaac ttctggattt ttaaaattca agtctacttg
    73561 atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt
    73621 aagtaacggg ctggtccttg gtctagtcac gctgcttgta tctgtatcca gtggcaaatc
    73681 aataaaaaaa tctttgtacg tatcattcaa gtttaaatat tcctgaaacc aagtactttc
    73741 ggcagacata tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc
    73801 tatatcttcc catgtgccca aacggtcgtt gattgcctga taatttacaa cggagaaacc
    73861 atcatcagat gtatatggga aaaatggcaa gaaatgtaca actctaaatg tatctgccaa
    73921 atacttttga taaaatttct taaatgtacg caaagttggt tgctgctctt caaaaaattg
    73981 atccccataa gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc
    74041 tttcttctgc tgataaacac tgactttttg ttcaatcata gaggcggttt tctcagacaa
    74101 atctgagtcg cgataaatcg cttgtaaaag ttttgtatac atcggattcc cctttctttt
    74161 ttgggagcgt tcccaacgat ttgatcatat tatggaaaac gcttactgtc aatagcattt
    74221 ttttaaataa ttttgatata cacaaaaata caaataagaa agcttatgta tcaataatta
    74281 atagcttctt aaaaaaatat ggtctctcat gatcatgcaa gctattccta aattttcaaa
    74341 atcatagaca aaaaagaatg tctattgtac tatggaatta ttctaaaacg ctcccaaaaa
    74401 ggaggtgcat ataaaattga aacctactat ttatgacatt gcgaaaaaag ctaatgtctc
    74461 aaaatcaact gtctccagag tattaaataa ccagccaaat atctctgata aagcaaaagc
    74521 aagtgtactt caagcaatca aagaattgaa ttatgaaccg agcaaaattg caagaggact
    74581 gtcaggaaat ggttttgacg cgattctagc attatctcat cgtacttctc atacgaccta
    74641 tggcaatcct tttttttcag atgttattca atccatttct gccgtttctg aacaaaataa
    74701 cttcgacttg attctacaaa cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa
    74761 aaagatccaa gaaaaaatga tccgaggaat tattatttta agctctacga tggatgaaac
    74821 acttcttttt gaattggatc aatataatat tcctattgtg gtaatcggaa aagtcggaaa
    74881 cgactacacg aacatctact cagtggacac ggataatttc caggatagtt acgatttggt
    74941 ggaaacactg atcaagaatg gacaccgtca tatcggctgt ctccatgctc cgacagaagt
    75001 ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc accacctaga
    75061 tatccgtaat gaatggatta tagacggcgg ctttcgctta gaagatagca tggcagcaat
    75121 gaatgattta atgaagctac gagaaagacc aactgcgatt ttttctaccg atgcattaaa
    75181 atcattgagt atttacaaag tggcagaaca atctggaaag cagatacctg atgatctttc
    75241 gatcgcagga ttcagtgatc ccttattgtc ctccctattt tcgccacagt tgactcggat
    75301 agacattccg acaaatcagt taggaatcaa agccacagaa cttttattcc aactaatcca
    75361 ccaccaatac cctgaagaaa aaagaatcat tctttcaacc gagattactc tcacagattc
    75421 gatccaacaa caagaaatgt agaacagaat tataaaagaa cagaaacact gaggataagt
    75481 cagttactct gttctttttt caatcactca tccctcgtta ccccctcccg gtattttagc
    75541 gaatttttct tattttccat ctgttttaga tagctgtgga taactataat gtttgtgtta
    75601 aactattttt tccacaggga agaaaaaact atttttaacc acgttgtgta taagttttta
    75661 tagttcgttt ttttaaacac atttaccctt atttcaatcc atgttatttg gggatactta
    75721 tctctaaaac ataaatagaa taacagacat gtctactggt tttgttttat tcgaggaatt
    75781 attcaataat agctagcaaa atattaaaaa taaccgctct atcttcccat caatttattt
    75841 tgcattcaaa aaatgatcta tctttacttt tgttgattga cttctcttgg aagatgatcg
    75901 ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca cttttcttct
    75961 agttagacaa attattttag acaaaccaaa aaagaggttg tgacaataat ttgtcacaac
    76021 ctctttttta aaatatctat tttgtttgat tgttcattgc gtcttctgca gccatttgcg
    76081 cttcaatatc tgaaatactg tagacatttt cgcttgctac accgacttct tttggttcca
    76141 tgtcacgata acgttgcata ccagtaccag ctgggatgat cttacctata ataacatttt
    76201 ctttcaaacc aagaagagga tctttcttac cgcggatcgc agcatccgtc aagacacgag
    76261 ttgtttcttg gaatgatgca gcagataaga aactatttgt ttctaatgat gcttttgtga
    76321 tacctagtaa gacaggtcga gaagttgcag gaacgcctcc tgagactagc gtattgtagt
    76381 tttgatcttt aaagtctgcg atgtctaata atgtacctgg taagatatct gtatcacctg
    76441 gatccattac acgaacttta cgcagcattt gacgaaccat tacttcgata tgcttgtcgc
    76501 cgatttctac cccttgcata cggtaaacac gttgtacttc acgaagcaag tagttttcaa
    76561 cagataatac atcgcgaact tgcaacaatt gttttggatc gattgatcct tctgtcaatg
    76621 gtgcaccacg gtggatcata tcgccttctg ctactttcat acgtgctgta taaggaacag
    76681 tatacgtacg tgtatctgtt tttcctttga ttgttacttc tttggtacgt gttgcaggat
    76741 cttctgagat atcgatgaca tcccctgtta cttctgtgat cactgcttgc cctttcggat
    76801 tacgtgcttc aaagatttct tggacacgag gaagaccttg agtgatatcg tctccggcaa
    76861 ccccacctgt atggaatgta cgcatcgtca actgagtacc aggttcaccg attgattgag
    76921 cggcgatcgt accaactgct tctccgactt caacgtcaga gccagttgca aggttgcgtc
    76981 cgtaacaatg tttgcagaca ccatgttttg tattacatgt gaagacagaa cggattgtca
    77041 ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc aattggtttc
    77101 ctgcaatgat caattcattt gtttcaggat gacgtacttc tttacgggtg taacgtccca
    77161 ctaaacgatc ttctaatgat tcgatgattt catttccctc gcggatggct tggatttcaa
    77221 gacctcggtc agtaccgcaa tcttcttcac ggatgattac atcttgggcc acgtcaacca
    77281 gacgacgagt caagtaacct gaatcggcag tcttcaaggc tgtatcggtc atccctttac
    77341 gagccccgtg agttgagata aacatttcca agacggatag tccttcacgg aagttcgaaa
    77401 tgattggcaa ttccatgatt cgaccattag gagcggccat caaaccacgc attccggcaa
    77461 gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc agacatcatg aagattggat
    77521 ttttcgcttc caaaccttcc atcagttttt gttggatgct gtcttttgca gcattccaaa
    77581 cagcaatgac acgttcataa cgttcgtcat ctgtgatcag accacgacgg aattgttttg
    77641 taatagtttc aacttgttta tgggcatttt caatgatttc ttgtttttca ttcagtacac
    77701 tgatgtcagc gatccctact gtgataccgg catgtgtcga atgtttgtac cctaagtctt
    77761 tcatacggtc aagcatctta gaagtttctg ttactttgaa tcgtttgaag acttctgcga
    77821 tgatgtttcc taagtttttc ttcttgaacg gtaatactaa ttcttgctct ttgatatgtg
    77881 caggaatatc agtaccagct tcaacaaagt atttgtccgg tgtttggacg gtcaagttga
    77941 agtctgttgg ttcattcaga taagggaatt caggaggcat gatctcgttg aagatgatct
    78001 taccaactgt tgtgatcatc gtacgttctt tttgccattc agtaaatggt ttttcaccta
    78061 atgagttagg gttcacaccg atacgtgaat gtaaatgaac atatccgttg cgccatgcga
    78121 taacggcttc gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct tcttcttcca
    78181 ttgtcaagta gtagttacct agaaccatat cttgagaagg agtaacaact ggtttaccat
    78241 ctttagggtt caagatgttt tgagcagcta acatcaacat acgtgcttct gcttgtgctt
    78301 cttcattcaa tggaacgtgg acagccattt ggtctccatc gaaatcggca ttataagctt
    78361 cacatacaag tggatgcaaa cggattgcgc gtccttgtac taaaactggt tcgaatgctt
    78421 ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt tccttgatta
    78481 cgtcttctaa aacatcccat acttcgtctt cttgacgttc gattttacgt ttcgcatttt
    78541 tgatgtttga agctagttcg cgttgaacaa gctcacgcat cacgaatggt ttgaataatt
    78601 caatggccat ttcttttgga agtccacatt ggtacatctt caagaaaggt ccaacaacga
    78661 taacggaacg acctgagtag tcgacacgtt tacctagcaa gttttgacgg aaacgacctt
    78721 gtttcccttt caacatatga gaaagagatt tcaatgggcg gttacctggt ccagtaactg
    78781 gacgtccgcg acgaccgtta tcgatcaatg catctactgc ttcttgcagc atacgttttt
    78841 cattttgaac gatgattcca ggtgcattca agtctaacaa acgtttcaaa cggttattac
    78901 ggttgattac acgacggtaa agatcattca agtcacttgt tgcaaaacgt ccaccttcta
    78961 attgaaccat tgggcgtaaa tctggtggaa taacagggat aacgtccata accatccagc
    79021 ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg attgcacgtg
    79081 tacgtttttg tccttgtgca gatttcaatt cttctttcaa ttcggctgct tctgcatcta
    79141 gatcaacatt atcaagtaat tgtttaatcg cttctgcacc catagcagct tggaattctt
    79201 gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt tttttctcta
    79261 aattcgtatt tcctggttcg atgactacat atgaagcaaa ataaatgatt tcttccaaag
    79321 cgcgtgggct catgtctaat acaagaccca tacgtgaagg aatcccttta aagtaccaga
    79381 tatgtgaaac aggagctgct aattcaatat gtcccatgcg ttcacggcga actttcgaac
    79441 gagtaacttc cactccgcat cgatcacaaa cgattccttt ataacggata cgtttgtatt
    79501 ttccgcaggc acattcccag tcttttgtag gaccaaaaat ccgctcacag aacaaccctt
    79561 cacgttcagg ttttaaggta cgatagttaa tcgtctcagg tttttttact tcaccgtaag
    79621 accagcttct gattttttcg ggagaagcca aacctatttg catactttcg aatttattta
    79681 catcgatcaa aaggggttcc ctccatttca tttaatggca aaccgcaaga cggctgatct
    79741 tcgaccgcct tgcagctcat agaccggatt agtctaaatt atcttctgcg gtttcaaagt
    79801 tttggatgac atcgtctttt tcatcttcca cttgttcagc agctttcttt tctaattctt
    79861 tggcagtttg ctgttcggca aattttgtca aagcatcaac agtgatcaaa tcgtcgtctt
    79921 catcgtccat gtcgcgaagt tcgatctctg tttcttcgat atccaagaca cgcatgtcca
    79981 gacctagtga ttgcaattct ttcaccaata cgcggaatga ttccggtaca cctggttttg
    80041 gaattggttc acccttgacg attgcttcgt atgttttcac acgtccgact acgtcatctg
    80101 atttgtatgt caagatttct tgtaaagtat aagccgcacc gtaagcttcc agtgcccata
    80161 cttccatttc accaaaacgt tgtccaccaa attgagcttt acctcccaat ggttgttgtg
    80221 taaccaatga gtaaggtccg attgaacgtg catgcaattt gtcatcgacc atgtgagcaa
    80281 gtttgatcat gtacatcaca ccgacagaca cacgtccatc aaatggttca cctgtacgtc
    80341 cgtcgtacag aacagttttc gcatcgctgg ccataccggc ttcacgaact gtttcccata
    80401 cgtcttcatc acttgcacca tcaaatactg gcgttgctac gtggatacct aactgacgtg
    80461 cagccatacc caagtgtaat tccaatactt gcccgatgtt catacgtgaa ggcaccccta
    80521 aaggattcag catgatatcg attggtgttc catcaggtag gaatggcata tcttcttccg
    80581 gcataatacg ggatacaacc cctttatttc cgtgacgacc ggccatttta tccccttcat
    80641 ggattttacg tttttgaacg atataaacac gaactaacat gtttacgcct ggtgacaatt
    80701 catctccagc ttcacgagta aagattttca catcgtgaac gataccgccg ccgccatgtg
    80761 gtacacgtaa tgatgtatcg cggacttcac gagctttttc gccaaagatc gcatgcaata
    80821 gacgttcttc tgcagataat tctgttaccc cttttggcgt gactttccct acaagcaaat
    80881 cgccatcttg gacttctgca ccaatacgga tgatacccat ttcgtctaaa tcttttaatg
    80941 cgtcttcccc gacattaggg atttcgcgtg tgatttcttc aggtcctaat tttgtatcgc
    81001 gggcttctga ttcatattct tcaatatgga tagaagtgta tacgtcatct tttaccaaac
    81061 gacggctcat gatgatcgca tcttcatagt tgtacccttc ccaagtcatg aaaccaacta
    81121 aaacgttttg gcctagagcc atttcgcctt gttccataga tggtccgtct gccagagtat
    81181 cccctttttc gactttctca cccaaatgaa cgattggacg ttggttgtag cttgttccgg
    81241 agtttgaacg acggaatttt gttacatcgt atttatctaa tgcaccgttg tcgcggcgta
    81301 cgcggatttc agaagcatca acgtattcaa cgacaccgtc atgtttacat aacaaagcag
    81361 ctcctgagtc atgcgctgat ttgtattcca taccagtacc aacccatggt gcttgaggat
    81421 tgatcaacgg cactgcttga cgctgcatgt tggcacccat caaggcacgg ttggagtcat
    81481 cgttttccaa gaaaggaata catgctgtcg caacggcaac tacttgttta ggtgatacgt
    81541 ccatgtagtc tactttatca atagatactt ctaagttttc actttgagca cgagccataa
    81601 caacgtcttg agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg gctacgatgt
    81661 aatggtcttc gatatcagct gtcaagtagt cgatttggtc agtaacacga cctgtttcac
    81721 ggtctacacg acgataaggt gtttcgataa atccgaattt atttactttc gcataactgg
    81781 acaaactatt gatcaacccg atgtttgggc cttcaggagt ttcgatcgga cacatacggc
    81841 catagtggga gtagtgaacg tcacgaactt cataaccggc gcggtcacga gtcaatccgc
    81901 caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg tttgtttgat
    81961 ccatgaactg tgacaactga gaagaaccaa agaattcttt gatacttgcc acaactggac
    82021 ggatattgat caattgttga ggtgtcaatg tttcagtatc ttggatcgac attctttcac
    82081 gaaccacacg ttccatacgt gctagcccga tacggaattg gttttgtaaa agttcaccga
    82141 ctgaacggat acgacgattt cctaagtggt cgatatcatc gacattgccg ataccttcca
    82201 tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc tggacgaacc gtttttacag
    82261 ctgcttctgg atagccgttt ccgatcacgt ttacttcacg ttcagggtct cttggtgaaa
    82321 atactttgat gacttggaca gtcattggat ctgtgaccac accgtcttct gatggatagt
    82381 aagtcactga atttaggcca ttatcaatga aaggagctag tgtttccatg acttggtgag
    82441 tcagaactgt tcctttttca acgatgatct cgcctgtttc tggatcaact aatgtttcag
    82501 ctaatgtcag attcaataga cgtgttttca aatctaattt tttgtttact ttgtaacgac
    82561 caacgtttgc taagtcgtaa cgttttggat cgaagaaacg agcattcagc aagttacgtg
    82621 agctgtctgc tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct ttcaaacctt
    82681 cttctgttct tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta ttgcgtaatg
    82741 tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga accaaatcct aacgcacgaa
    82801 ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg tacataagag atgtcttttg
    82861 catcagtttc catttctagc caagctccac ggttagggat aacagtagaa ccgaatcctt
    82921 cttttccgtt tttgtcgact tttccatgga aatagacacc tggtgaacgg actaactgag
    82981 aaacgattac tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc attaatggga
    83041 aatcaccgaa gaatacttct tgtgctttga tttcgccagt ctcgcggttt gtcaaacgca
    83101 atgttacatg taatggtgca gagtagtttg catcatgcgc acgagcttct gctactgtat
    83161 actttggttc tttcaattca taatctacaa attccaaaga tagattgcca ttgaagtcat
    83221 cgattggcaa aatgtcttca aacatttctc taagtccctc atctaagaac cattggtaag
    83281 agtctgtttg aatttcgatc aaatttggta attccaacac ttcactgatt cttgcgaaac
    83341 ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc ttcacccctc
    83401 taaaatgtca ttcaaaaaga ctacccgatc aattgttaca ataatatttc aaatattatt
    83461 tttaggtaac aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa aaatagaaca
    83521 ttctcttgaa accttacttc gtgagcttct acttttgttt ccttatatta aagccgaaac
    83581 ggacaatatt tcgtttctgc cttttcttca tcagatagtt tttgcaactt catatactac
    83641 acttattttc tataaaagtc aacacttcct ggcgtttctt ctgctttttc gcaacatttt
    83701 cttaatgttt tcaattttat gttttcaatt atctgacaat tcttaattga cagacctttt
    83761 tatctcggtt atactttaga aaattcattt tgtggaggaa cttctatgcg aaccaacatc
    83821 aaccaaatcg caccaacaca actgaataac ttttacttta gctactttag ataggtttgt
    83881 attcatcatg atggatacaa acgttaaacg acagtttgta tctatgatgg ctaaagaact
    83941 ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag tgaaaataca
    84001 aagtaaaagt atcaagcatt ctactttttc aatttatttt ccgacttttc aatccatttg
    84061 gacattttgt ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa gaaatgaaaa
    84121 aaaatataat catcggatta ggcgctgccc tgctactagc tttaggaggt tgcgcacaaa
    84181 aagacaattc cgcagaggca tcagatggac aaaaaacaat tggcgtattg caacttgtac
    84241 aacataactc gttagatgct gcctataaag gattcaaaga aggattagca gaagaagggt
    84301 ataaagaagg cgataatcta acaatcgatt atcaaaatgc ccaaaacaat caagacaacc
    84361 tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga tttgctactt ggaatcgcca
    84421 ctccagcagc acaatcttta ttaaatgaaa caaccactat cccgatgact gtcacagcgg
    84481 tcacagactt ggaacaagcc catttagtca catccaatga aaaacctggc ggcaatgtga
    84541 ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg cttactgtta aatatcgtgc
    84601 cagacgccaa aacaatcggc atcatgtata acaacggtga agcaaactct aagatccaag
    84661 ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta ctgacagcaa
    84721 attccacaaa tgatgtccaa caagtgacga aaagtctagc taaagatgta gatggaatct
    84781 atattcctac tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa gtcgcaaaag
    84841 aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt caaagacggc ggtctagcaa
    84901 cagtaggaat cgactacgaa agcttgggca aacaaacagg gatcatggca gcaaaaatct
    84961 tagacggcaa gaatccaagt gaacttcctg tcgaaacagc gaatgatttg gaactgtatg
    85021 tcaataaaga catggccaaa gcattaggca tcaatccaga cagcatcaaa gaagctgaat
    85081 aaaacacagg aggattctca tgacaaattt gctctctctt ttcttatcat ctgcatcgca
    85141 aggggtctta tgggccctac ttgcgattgg tgtatacgtc actttccgta tcttggacat
    85201 tgccgacttg actgctgaag gaagctttcc tttaggtgca gggatcgcgg cagtaagtat
    85261 tacaaatggc tatcatccat tgacagcttg tcttctaggc tttttagggg gtgcggcagc
    85321 agggcttgtt tccggactgc ttcacacaaa attgaaaatt cccgcccttc ttgcagggat
    85381 catcaccatg actggtttat attctgtcac atcccgtgtg atgggtgcgc cgaacatctc
    85441 tttattgggt cagccaacga tctttcattg ggtcgagtca ttcggcataa caaaagacaa
    85501 cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac ttgttttgtt
    85561 cttacgtaca gaaacaggac ttgctattcg tgcaactggc gataatctgg caatgagcga
    85621 agcaaacggg atcaacacag ataatatgaa gatcatcggt tatatgattt ctaatggatg
    85681 cattgctctt tctggctcct tacttgcgca aaacaacggc tttgctgatt taaattctgg
    85741 tattggaacg atcgtaattg gacttgcttc tattatcatc gcagaagttt tgttacgtaa
    85801 ccagccacta tttctccgtc tcttgactgt cgttttaggt gcggtcattt atcgttttat
    85861 tttagcgtta gtcttcgaac tgaatgttga accaagtgat tcgaaacttg cttctgcttt
    85921 cgtgctagtg atctgtcttt ctttcccgca gatccagcaa aaattccatt tacctcaaaa
    85981 aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat tggaaaatct ccatcatacc
    86041 tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct ttccttagat
    86101 cccggcgact tcgtgacgat catcggtgga aatggggctg gtaaatctac tttgctgaac
    86161 agcatcgccg gtacattttc tgtcgatcag ggtcaaattt tattgaatgg aaaaaatatc
    86221 acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca agatcctaaa
    86281 ctagggacag ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat gaaacgagga
    86341 aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc gaacattttt caaagaccag
    86401 ctggttcggc taaatttggg gttggaaaat cgtttatcct cagaaatcgg tttgctgtct
    86461 ggaggacaac gtcaagctat tacgttattg atggccacat tacagcgtcc agaactgatc
    86521 ttattagatg aacacactgc agcgcttgac cctgctactt ccaaaacggt gatggaattg
    86581 acagaaaagc tgatcactga gcagaatctc actgctttta tggtcactca tgacatggaa
    86641 gacgcgatcc gctatgggag tcgactgatc atgctgcatc aaggaaggat cgtcgtagat
    86701 atccagcaag aggaaaaaca gcagctcaca gtgccccaat tattagcgtt gttcaaacaa
    86761 aacagcggca cggcattaaa agacgatcaa gtacttttat cttcataatc agaaagagga
    86821 tgggacaaaa aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct
    86881 ttatgtatca accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa
    86941 tggatgggaa aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa
    87001 aagacaccag attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca
    87061 agtatttgga aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct
    87121 acagggtatc gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta
    87181 cggtcgttta ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa
    87241 gatgccaaac tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca
    87301 caagaacaag ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc
    87361 tacatgagtc tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc
    87421 atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc
    87481 aaatgggtca atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca
    87541 gtcagtgata tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt
    87601 tctgttcctc aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc
    87661 gacggacaag ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc
    87721 acttatcttt ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg
    87781 ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc
    87841 ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc
    87901 gaaatcagtc tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg
    87961 atctcataca gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc
    88021 tgacagtttc tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca
    88081 ctttctttaa atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga
    88141 gatagcaggc cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa
    88201 aaaagaactt gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta
    88261 atcccaacaa acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa
    88321 tagaaacgag acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat
    88381 ctagctcgga aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca
    88441 agtatcctag cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat
    88501 gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac
    88561 tatgccaaat tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg
    88621 tatgccaagc cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac
    88681 gattggcctc ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat
    88741 ccacactctc gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct
    88801 acttactttt tcctaattct aggataccta cttatatgtt actacacacc cttatttttt
    88861 acactttaac agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa
    88921 ctatcaattg aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa
    88981 tgatttttca aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg
    89041 aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt
    89101 tgttcaatat cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc
    89161 ctgggaatat ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat
    89221 tccacgtatt tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa
    89281 cacatgaact tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc
    89341 aagaaataaa ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag
    89401 gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg
    89461 gtcatcctac agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag
    89521 atcgtgctgg gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag
    89581 caaatgatct tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca
    89641 tgaacaatcg gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata
    89701 tcgatgcaga agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag
    89761 tagttgtatt taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat
    89821 acaatgatcg ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt
    89881 ttggaaatga gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc
    89941 ctaatgctac tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga
    90001 ttttagatgc agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta
    90061 cagatacgac agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt
    90121 catca
//
LOCUS       DASHFF010000005        76211 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.005, whole genome shotgun sequence.
ACCESSION   DASHFF010000005 DASHFF010000000
VERSION     DASHFF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.005"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..411
                     /locus_tag="U2I75_000374"
     CDS             <1..411
                     /locus_tag="U2I75_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1695537.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="U2I75_000375"
     CDS             509..697
                     /locus_tag="U2I75_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN1695538.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="U2I75_000376"
     CDS             complement(755..1108)
                     /locus_tag="U2I75_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1695539.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="U2I75_000377"
     CDS             1639..2061
                     /locus_tag="U2I75_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1695540.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="U2I75_000378"
     CDS             2058..2288
                     /locus_tag="U2I75_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1695541.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="U2I75_000379"
     CDS             2786..2986
                     /locus_tag="U2I75_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1695542.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="U2I75_000380"
     CDS             3013..4206
                     /locus_tag="U2I75_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1695543.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="U2I75_000381"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="U2I75_000381"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN1695544.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="U2I75_000382"
     CDS             complement(5898..6821)
                     /locus_tag="U2I75_000382"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN1695545.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="U2I75_000383"
     CDS             7017..7622
                     /locus_tag="U2I75_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1695546.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="U2I75_000384"
     CDS             7930..8946
                     /locus_tag="U2I75_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1695547.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="U2I75_000385"
     CDS             9087..10388
                     /locus_tag="U2I75_000385"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN1695548.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="U2I75_000386"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="U2I75_000386"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN1695549.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="U2I75_000387"
     CDS             11117..11515
                     /locus_tag="U2I75_000387"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN1695550.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="U2I75_000388"
     CDS             11581..12663
                     /locus_tag="U2I75_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1695551.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="U2I75_000389"
     CDS             12875..14437
                     /locus_tag="U2I75_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695552.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="U2I75_000390"
     CDS             14430..15563
                     /locus_tag="U2I75_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695553.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="U2I75_000391"
     CDS             15563..16519
                     /locus_tag="U2I75_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695554.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="U2I75_000392"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="U2I75_000392"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN1695555.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="U2I75_000393"
     CDS             17808..18626
                     /locus_tag="U2I75_000393"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1695556.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="U2I75_000394"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="U2I75_000394"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1695557.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="U2I75_000395"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="U2I75_000395"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1695558.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="U2I75_000396"
     CDS             complement(20506..21087)
                     /locus_tag="U2I75_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1695559.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="U2I75_000397"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="U2I75_000397"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN1695560.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="U2I75_000398"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="U2I75_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN1695561.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="U2I75_000399"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="U2I75_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN1695562.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="U2I75_000400"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="U2I75_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1695563.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="U2I75_000401"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="U2I75_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1695564.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27136..27444
                     /gene="rpsJ"
                     /locus_tag="U2I75_000402"
     CDS             27136..27444
                     /gene="rpsJ"
                     /locus_tag="U2I75_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN1695565.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27475..28104
                     /gene="rplC"
                     /locus_tag="U2I75_000403"
     CDS             27475..28104
                     /gene="rplC"
                     /locus_tag="U2I75_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN1695566.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28132..28755
                     /gene="rplD"
                     /locus_tag="U2I75_000404"
     CDS             28132..28755
                     /gene="rplD"
                     /locus_tag="U2I75_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN1695567.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28755..29045
                     /gene="rplW"
                     /locus_tag="U2I75_000405"
     CDS             28755..29045
                     /gene="rplW"
                     /locus_tag="U2I75_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN1695568.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29084..29917
                     /gene="rplB"
                     /locus_tag="U2I75_000406"
     CDS             29084..29917
                     /gene="rplB"
                     /locus_tag="U2I75_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN1695569.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29960..30238
                     /gene="rpsS"
                     /locus_tag="U2I75_000407"
     CDS             29960..30238
                     /gene="rpsS"
                     /locus_tag="U2I75_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN1695570.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30261..30608
                     /gene="rplV"
                     /locus_tag="U2I75_000408"
     CDS             30261..30608
                     /gene="rplV"
                     /locus_tag="U2I75_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN1695571.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30622..31278
                     /gene="rpsC"
                     /locus_tag="U2I75_000409"
     CDS             30622..31278
                     /gene="rpsC"
                     /locus_tag="U2I75_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN1695572.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31281..31715
                     /gene="rplP"
                     /locus_tag="U2I75_000410"
     CDS             31281..31715
                     /gene="rplP"
                     /locus_tag="U2I75_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN1695573.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31705..31893
                     /gene="rpmC"
                     /locus_tag="U2I75_000411"
     CDS             31705..31893
                     /gene="rpmC"
                     /locus_tag="U2I75_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN1695574.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31918..32184
                     /gene="rpsQ"
                     /locus_tag="U2I75_000412"
     CDS             31918..32184
                     /gene="rpsQ"
                     /locus_tag="U2I75_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN1695575.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32242..32610
                     /gene="rplN"
                     /locus_tag="U2I75_000413"
     CDS             32242..32610
                     /gene="rplN"
                     /locus_tag="U2I75_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN1695576.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32647..32955
                     /gene="rplX"
                     /locus_tag="U2I75_000414"
     CDS             32647..32955
                     /gene="rplX"
                     /locus_tag="U2I75_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN1695577.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32983..33522
                     /gene="rplE"
                     /locus_tag="U2I75_000415"
     CDS             32983..33522
                     /gene="rplE"
                     /locus_tag="U2I75_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN1695578.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33541..33726
                     /locus_tag="U2I75_000416"
     CDS             33541..33726
                     /locus_tag="U2I75_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN1695579.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33762..34160
                     /gene="rpsH"
                     /locus_tag="U2I75_000417"
     CDS             33762..34160
                     /gene="rpsH"
                     /locus_tag="U2I75_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN1695580.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34192..34728
                     /gene="rplF"
                     /locus_tag="U2I75_000418"
     CDS             34192..34728
                     /gene="rplF"
                     /locus_tag="U2I75_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN1695581.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34889..35245
                     /gene="rplR"
                     /locus_tag="U2I75_000419"
     CDS             34889..35245
                     /gene="rplR"
                     /locus_tag="U2I75_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN1695582.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35266..35766
                     /gene="rpsE"
                     /locus_tag="U2I75_000420"
     CDS             35266..35766
                     /gene="rpsE"
                     /locus_tag="U2I75_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN1695583.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35781..35960
                     /gene="rpmD"
                     /locus_tag="U2I75_000421"
     CDS             35781..35960
                     /gene="rpmD"
                     /locus_tag="U2I75_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN1695584.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36004..36444
                     /gene="rplO"
                     /locus_tag="U2I75_000422"
     CDS             36004..36444
                     /gene="rplO"
                     /locus_tag="U2I75_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN1695585.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36444..37739
                     /gene="secY"
                     /locus_tag="U2I75_000423"
     CDS             36444..37739
                     /gene="secY"
                     /locus_tag="U2I75_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN1695586.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37801..38448
                     /locus_tag="U2I75_000424"
     CDS             37801..38448
                     /locus_tag="U2I75_000424"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN1695587.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38639..38857
                     /gene="infA"
                     /locus_tag="U2I75_000425"
     CDS             38639..38857
                     /gene="infA"
                     /locus_tag="U2I75_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN1695588.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38890..39006
                     /gene="rpmJ"
                     /locus_tag="U2I75_000426"
     CDS             38890..39006
                     /gene="rpmJ"
                     /locus_tag="U2I75_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN1695589.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39024..39389
                     /gene="rpsM"
                     /locus_tag="U2I75_000427"
     CDS             39024..39389
                     /gene="rpsM"
                     /locus_tag="U2I75_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN1695590.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39417..39806
                     /gene="rpsK"
                     /locus_tag="U2I75_000428"
     CDS             39417..39806
                     /gene="rpsK"
                     /locus_tag="U2I75_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN1695591.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39886..40824
                     /locus_tag="U2I75_000429"
     CDS             39886..40824
                     /locus_tag="U2I75_000429"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN1695592.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40852..41232
                     /gene="rplQ"
                     /locus_tag="U2I75_000430"
     CDS             40852..41232
                     /gene="rplQ"
                     /locus_tag="U2I75_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN1695593.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41504..42343
                     /locus_tag="U2I75_000431"
     CDS             41504..42343
                     /locus_tag="U2I75_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1695594.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42319..43188
                     /locus_tag="U2I75_000432"
     CDS             42319..43188
                     /locus_tag="U2I75_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1695595.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43178..43975
                     /locus_tag="U2I75_000433"
     CDS             43178..43975
                     /locus_tag="U2I75_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1695596.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44178..44924
                     /gene="truA"
                     /locus_tag="U2I75_000434"
     CDS             44178..44924
                     /gene="truA"
                     /locus_tag="U2I75_000434"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1695597.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45257..47926)
                     /locus_tag="U2I75_000435"
     CDS             complement(45257..47926)
                     /locus_tag="U2I75_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN1695598.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48164..48352)
                     /locus_tag="U2I75_000436"
     CDS             complement(48164..48352)
                     /locus_tag="U2I75_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN1695599.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48622..48984
                     /locus_tag="U2I75_000437"
     CDS             48622..48984
                     /locus_tag="U2I75_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1695600.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49036..50718
                     /locus_tag="U2I75_000438"
     CDS             49036..50718
                     /locus_tag="U2I75_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN1695601.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            50835..52871
                     /gene="recG"
                     /locus_tag="U2I75_000439"
     CDS             50835..52871
                     /gene="recG"
                     /locus_tag="U2I75_000439"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN1695602.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            52920..53921
                     /gene="plsX"
                     /locus_tag="U2I75_000440"
     CDS             52920..53921
                     /gene="plsX"
                     /locus_tag="U2I75_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN1695603.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54043..54285
                     /gene="acpP"
                     /locus_tag="U2I75_000441"
     CDS             54043..54285
                     /gene="acpP"
                     /locus_tag="U2I75_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1695604.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            54553..55557
                     /locus_tag="U2I75_000442"
     CDS             54553..55557
                     /locus_tag="U2I75_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695605.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            55558..56502
                     /locus_tag="U2I75_000443"
     CDS             55558..56502
                     /locus_tag="U2I75_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695606.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56502..57464
                     /locus_tag="U2I75_000444"
     CDS             56502..57464
                     /locus_tag="U2I75_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695607.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57491..58405
                     /locus_tag="U2I75_000445"
     CDS             57491..58405
                     /locus_tag="U2I75_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695608.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58431..60212
                     /locus_tag="U2I75_000446"
     CDS             58431..60212
                     /locus_tag="U2I75_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1695609.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            60560..61246
                     /locus_tag="U2I75_000447"
     CDS             60560..61246
                     /locus_tag="U2I75_000447"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN1695610.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61266..64847
                     /gene="smc"
                     /locus_tag="U2I75_000448"
     CDS             61266..64847
                     /gene="smc"
                     /locus_tag="U2I75_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN1695611.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            64856..65665
                     /locus_tag="U2I75_000449"
     CDS             64856..65665
                     /locus_tag="U2I75_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1695612.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            65676..66674
                     /gene="ftsY"
                     /locus_tag="U2I75_000450"
     CDS             65676..66674
                     /gene="ftsY"
                     /locus_tag="U2I75_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN1695613.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            66861..69128
                     /gene="gshAB"
                     /locus_tag="U2I75_000451"
     CDS             66861..69128
                     /gene="gshAB"
                     /locus_tag="U2I75_000451"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN1695614.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69217..69669)
                     /locus_tag="U2I75_000452"
     CDS             complement(69217..69669)
                     /locus_tag="U2I75_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN1695615.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            69819..70769
                     /locus_tag="U2I75_000453"
     CDS             69819..70769
                     /locus_tag="U2I75_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1695616.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            70762..71721
                     /locus_tag="U2I75_000454"
     CDS             70762..71721
                     /locus_tag="U2I75_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1695617.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            71718..72473
                     /locus_tag="U2I75_000455"
     CDS             71718..72473
                     /locus_tag="U2I75_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1695618.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72496..73449
                     /locus_tag="U2I75_000456"
     CDS             72496..73449
                     /locus_tag="U2I75_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1695619.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(74093..74389)
                     /locus_tag="U2I75_000457"
     CDS             complement(74093..74389)
                     /locus_tag="U2I75_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN1695620.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            74744..75100
                     /locus_tag="U2I75_000458"
     CDS             74744..75100
                     /locus_tag="U2I75_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1695621.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            75110..76009
                     /locus_tag="U2I75_000459"
     CDS             75110..76009
                     /locus_tag="U2I75_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN1695622.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        24831 a        13781 c        16342 g        21257 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    26821 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    26881 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    26941 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27001 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27061 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27121 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27181 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27241 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27301 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27361 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27421 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27481 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27541 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    27601 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    27661 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    27721 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    27781 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    27841 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    27901 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    27961 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28021 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28081 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28141 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28201 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28261 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28321 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28381 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28441 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28501 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28561 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    28621 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    28681 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    28741 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    28801 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    28861 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    28921 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    28981 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29041 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29101 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29161 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29221 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29281 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29341 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29401 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29461 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29521 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    29581 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    29641 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    29701 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    29761 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    29821 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    29881 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    29941 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30001 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30061 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30121 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    30181 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    30241 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    30301 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    30361 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    30421 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    30481 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    30541 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    30601 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    30661 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    30721 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    30781 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    30841 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    30901 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    30961 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    31021 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    31081 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    31141 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    31201 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    31261 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    31321 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    31381 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    31441 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    31501 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    31561 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    31621 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    31681 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    31741 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    31801 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    31861 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    31921 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    31981 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    32041 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    32101 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    32161 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    32221 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    32281 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    32341 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    32401 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    32461 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    32521 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    32581 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    32641 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    32701 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    32761 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    32821 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    32881 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    32941 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    33001 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    33061 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    33121 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    33181 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    33241 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    33301 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    33361 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    33421 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    33481 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    33541 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    33601 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    33661 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    33721 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    33781 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    33841 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    33901 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    33961 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    34021 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    34081 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    34141 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    34201 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    34261 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    34321 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    34381 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    34441 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    34501 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    34561 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    34621 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    34681 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    34741 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    34801 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    34861 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    34921 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    34981 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    35041 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    35101 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    35161 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    35221 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    35281 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    35341 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    35401 gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    35461 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    35521 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    35581 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    35641 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    35701 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    35761 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    35821 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    35881 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    35941 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    36001 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    36061 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    36121 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    36181 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    36241 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    36301 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    36361 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    36421 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    36481 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    36541 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    36601 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    36661 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    36721 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    36781 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    36841 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    36901 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    36961 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    37021 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    37081 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    37141 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    37201 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    37261 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    37321 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    37381 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    37441 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    37501 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    37561 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    37621 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    37681 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    37741 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    37801 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    37861 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    37921 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    37981 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    38041 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    38101 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    38161 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    38221 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    38281 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    38341 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    38401 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    38461 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    38521 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    38581 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    38641 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    38701 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    38761 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    38821 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    38881 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    38941 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    39001 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    39061 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    39121 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    39181 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    39241 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    39301 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    39361 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    39421 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    39481 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    39541 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    39601 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    39661 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    39721 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    39781 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    39841 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    39901 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    39961 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    40021 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    40081 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    40141 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    40201 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    40261 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    40321 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    40381 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    40441 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    40501 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    40561 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    40621 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    40681 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    40741 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    40801 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    40861 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    40921 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    40981 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    41041 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    41101 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    41161 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    41221 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    41281 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    41341 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    41401 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    41461 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    41521 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    41581 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    41641 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    41701 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatgat
    41761 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    41821 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    41881 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    41941 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    42001 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    42061 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    42121 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    42181 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    42241 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    42301 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    42361 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    42421 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    42481 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    42541 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    42601 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    42661 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    42721 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    42781 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    42841 ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    42901 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    42961 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    43021 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    43081 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    43141 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    43201 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    43261 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    43321 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    43381 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    43441 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    43501 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    43561 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    43621 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    43681 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    43741 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    43801 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    43861 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    43921 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt
    43981 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt
    44041 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    44101 tgaaaagaaa gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga
    44161 acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt
    44221 ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat
    44281 tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg
    44341 gagtacatgc gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac
    44401 gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa
    44461 ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag
    44521 tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc
    44581 cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca
    44641 cctctttttg tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta
    44701 agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct
    44761 tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc
    44821 ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag
    44881 cacaccctga aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag
    44941 tggtcagctt caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa
    45001 tttcggctta ttttcgaaga agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc
    45061 ctgtcattta actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg
    45121 aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt
    45181 tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac
    45241 aggttccatc gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt
    45301 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
    45361 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
    45421 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
    45481 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
    45541 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
    45601 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
    45661 caacatctag accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac
    45721 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
    45781 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
    45841 ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
    45901 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
    45961 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
    46021 aatactgttc atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
    46081 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
    46141 cattgacatt ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt
    46201 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca
    46261 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
    46321 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
    46381 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
    46441 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
    46501 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
    46561 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
    46621 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
    46681 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
    46741 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacca tcgtcttgtt
    46801 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
    46861 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
    46921 gataaaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
    46981 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
    47041 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
    47101 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
    47161 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
    47221 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
    47281 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
    47341 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
    47401 gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg
    47461 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
    47521 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
    47581 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
    47641 gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
    47701 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
    47761 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
    47821 ttccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
    47881 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
    47941 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
    48001 tctagttttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
    48061 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
    48121 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
    48181 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
    48241 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
    48301 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
    48361 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
    48421 tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
    48481 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
    48541 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
    48601 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
    48661 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
    48721 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
    48781 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
    48841 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
    48901 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
    48961 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
    49021 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
    49081 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
    49141 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
    49201 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
    49261 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
    49321 tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact
    49381 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
    49441 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
    49501 gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg
    49561 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
    49621 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
    49681 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
    49741 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
    49801 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
    49861 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
    49921 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
    49981 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
    50041 gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
    50101 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg
    50161 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
    50221 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
    50281 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
    50341 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
    50401 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
    50461 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
    50521 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
    50581 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
    50641 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
    50701 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
    50761 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga
    50821 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
    50881 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
    50941 attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag
    51001 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
    51061 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
    51121 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
    51181 aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg
    51241 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
    51301 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
    51361 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
    51421 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
    51481 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
    51541 tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa
    51601 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
    51661 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
    51721 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
    51781 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga
    51841 ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg
    51901 aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
    51961 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
    52021 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
    52081 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
    52141 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
    52201 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
    52261 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
    52321 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
    52381 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
    52441 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
    52501 tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
    52561 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
    52621 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
    52681 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
    52741 atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag gattggcaga
    52801 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
    52861 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
    52921 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
    52981 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    53041 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    53101 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    53161 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    53221 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    53281 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    53341 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    53401 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    53461 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    53521 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    53581 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    53641 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    53701 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    53761 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    53821 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    53881 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagcg
    53941 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    54001 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    54061 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    54121 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    54181 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    54241 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    54301 aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct ttttgacacg
    54361 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    54421 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    54481 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    54541 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    54601 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    54661 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    54721 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    54781 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    54841 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    54901 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    54961 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    55021 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    55081 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    55141 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    55201 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    55261 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    55321 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    55381 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    55441 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    55501 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    55561 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    55621 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    55681 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
    55741 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    55801 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    55861 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    55921 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    55981 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    56041 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    56101 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    56161 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    56221 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    56281 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    56341 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    56401 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    56461 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    56521 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    56581 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    56641 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    56701 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    56761 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    56821 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    56881 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    56941 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    57001 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    57061 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    57121 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    57181 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    57241 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    57301 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    57361 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    57421 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    57481 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    57541 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    57601 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    57661 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    57721 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    57781 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    57841 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    57901 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    57961 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    58021 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    58081 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    58141 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    58201 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    58261 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    58321 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    58381 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    58441 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    58501 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    58561 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    58621 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    58681 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    58741 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    58801 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    58861 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    58921 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    58981 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    59041 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    59101 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    59161 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    59221 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    59281 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    59341 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    59401 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    59461 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    59521 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    59581 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    59641 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    59701 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    59761 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
    59821 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
    59881 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    59941 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    60001 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    60061 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    60121 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    60181 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    60241 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    60301 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    60361 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    60421 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    60481 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    60541 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    60601 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    60661 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    60721 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    60781 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    60841 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    60901 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    60961 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    61021 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    61081 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    61141 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
    61201 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    61261 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    61321 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
    61381 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    61441 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    61501 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    61561 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    61621 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
    61681 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    61741 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    61801 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    61861 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    61921 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
    61981 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    62041 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    62101 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    62161 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    62221 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    62281 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
    62341 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
    62401 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
    62461 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
    62521 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    62581 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    62641 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    62701 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    62761 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    62821 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    62881 aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    62941 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    63001 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    63061 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    63121 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    63181 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    63241 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    63301 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    63361 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    63421 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
    63481 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    63541 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    63601 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    63661 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    63721 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    63781 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    63841 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    63901 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
    63961 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    64021 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    64081 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    64141 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    64201 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    64261 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    64321 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    64381 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    64441 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    64501 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    64561 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    64621 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    64681 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    64741 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    64801 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    64861 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    64921 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    64981 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    65041 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    65101 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    65161 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    65221 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    65281 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    65341 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    65401 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    65461 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    65521 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    65581 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    65641 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    65701 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    65761 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    65821 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    65881 gttttgaaac agcgatacga attaccgaag cattacgtca agaagtgaaa ctcagaaatg
    65941 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    66001 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    66061 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    66121 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    66181 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    66241 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    66301 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    66361 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    66421 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    66481 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    66541 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    66601 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    66661 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    66721 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    66781 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    66841 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    66901 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    66961 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    67021 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    67081 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    67141 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    67201 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaag
    67261 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    67321 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    67381 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    67441 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    67501 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    67561 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    67621 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    67681 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    67741 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    67801 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    67861 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    67921 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    67981 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    68041 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    68101 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    68161 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    68221 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    68281 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    68341 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    68401 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    68461 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    68521 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    68581 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    68641 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    68701 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    68761 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    68821 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    68881 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    68941 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    69001 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    69061 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    69121 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    69181 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    69241 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    69301 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    69361 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    69421 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    69481 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    69541 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    69601 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    69661 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    69721 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    69781 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    69841 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    69901 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    69961 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    70021 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    70081 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    70141 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    70201 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    70261 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    70321 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    70381 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    70441 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    70501 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    70561 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    70621 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    70681 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    70741 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    70801 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    70861 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    70921 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    70981 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    71041 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    71101 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    71161 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    71221 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    71281 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    71341 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    71401 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    71461 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    71521 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    71581 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    71641 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    71701 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    71761 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa attgactgct tttatcggtc
    71821 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    71881 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    71941 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    72001 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    72061 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    72121 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    72181 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    72241 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    72301 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    72361 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    72421 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    72481 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    72541 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    72601 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    72661 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    72721 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    72781 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    72841 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    72901 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    72961 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    73021 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    73081 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    73141 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    73201 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    73261 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    73321 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    73381 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    73441 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    73501 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    73561 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    73621 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    73681 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    73741 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    73801 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    73861 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacgatgttc
    73921 catctgctgc ttctcggcaa taagcccact agactagaaa atatgagaag ctattttctg
    73981 tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt
    74041 caatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat
    74101 gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa
    74161 gcttactcca ccgttttaat aattgataga aataactctc ttctgctttg attttatact
    74221 cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca
    74281 atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga
    74341 tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc
    74401 caaagaagtc ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac
    74461 aaaaagtaat tctttttcca atagttgtta aaggtttttt attttttata gtattttttg
    74521 ttatttaaga aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt
    74581 tcaagaaact gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat
    74641 aaacgaatga cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca
    74701 tctgatgaat aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac
    74761 aaaagaagca caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga
    74821 tatcgaaaac ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc
    74881 atttcgattg atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt
    74941 ggctgataca ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga
    75001 tccatccaca cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg
    75061 cttgagaaaa ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt
    75121 ggcgatcatc agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca
    75181 gctttggcaa gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc
    75241 taataaagta gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac
    75301 gacatttcat ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca
    75361 ttttccagac ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct
    75421 tttagggatg aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat
    75481 cgtacgaatg aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct
    75541 cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa
    75601 aaaacttgag gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca
    75661 atctgctcct tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg
    75721 agaattactt gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg
    75781 ctttgaagat aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta
    75841 tgaatggcag ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc
    75901 tatgaaatta cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca
    75961 acatttgcaa gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag
    76021 taatgtgtaa aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt
    76081 tatcgtggat atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat
    76141 gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac
    76201 aaaaaaaaat a
//
LOCUS       DASHFF010000006        75915 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.006, whole genome shotgun sequence.
ACCESSION   DASHFF010000006 DASHFF010000000
VERSION     DASHFF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.006"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(99..3377)
                     /locus_tag="U2I75_000460"
     CDS             complement(99..3377)
                     /locus_tag="U2I75_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN1695623.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3648..4193)
                     /locus_tag="U2I75_000461"
     CDS             complement(3648..4193)
                     /locus_tag="U2I75_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1695624.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4205..4897)
                     /locus_tag="U2I75_000462"
     CDS             complement(4205..4897)
                     /locus_tag="U2I75_000462"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN1695625.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(4916..5200)
                     /locus_tag="U2I75_000463"
     CDS             complement(4916..5200)
                     /locus_tag="U2I75_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695626.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5213..5776)
                     /locus_tag="U2I75_000464"
     CDS             complement(5213..5776)
                     /locus_tag="U2I75_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1695627.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6004..6666
                     /locus_tag="U2I75_000465"
     CDS             6004..6666
                     /locus_tag="U2I75_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN1695628.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            6790..7986
                     /locus_tag="U2I75_000466"
     CDS             6790..7986
                     /locus_tag="U2I75_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN1695629.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8087..9463)
                     /locus_tag="U2I75_000467"
     CDS             complement(8087..9463)
                     /locus_tag="U2I75_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1695630.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9500..10366)
                     /locus_tag="U2I75_000468"
     CDS             complement(9500..10366)
                     /locus_tag="U2I75_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEN1695631.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10455..11480)
                     /locus_tag="U2I75_000469"
     CDS             complement(10455..11480)
                     /locus_tag="U2I75_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1695632.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11498..12172)
                     /locus_tag="U2I75_000470"
     CDS             complement(11498..12172)
                     /locus_tag="U2I75_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1695633.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12356..13249)
                     /locus_tag="U2I75_000471"
     CDS             complement(12356..13249)
                     /locus_tag="U2I75_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1695634.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13323..15020)
                     /locus_tag="U2I75_000472"
     CDS             complement(13323..15020)
                     /locus_tag="U2I75_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1695635.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(15013..16326)
                     /locus_tag="U2I75_000473"
     CDS             complement(15013..16326)
                     /locus_tag="U2I75_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN1695636.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(16326..19844)
                     /locus_tag="U2I75_000474"
     CDS             complement(16326..19844)
                     /locus_tag="U2I75_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN1695637.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(20137..20595)
                     /locus_tag="U2I75_000475"
     CDS             complement(20137..20595)
                     /locus_tag="U2I75_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1695638.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(20893..21411)
                     /locus_tag="U2I75_000476"
     CDS             complement(20893..21411)
                     /locus_tag="U2I75_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN1695639.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(21413..22030)
                     /locus_tag="U2I75_000477"
     CDS             complement(21413..22030)
                     /locus_tag="U2I75_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN1695640.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(22027..22881)
                     /locus_tag="U2I75_000478"
     CDS             complement(22027..22881)
                     /locus_tag="U2I75_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1695641.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(22878..23789)
                     /locus_tag="U2I75_000479"
     CDS             complement(22878..23789)
                     /locus_tag="U2I75_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1695642.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(23830..25164)
                     /locus_tag="U2I75_000480"
     CDS             complement(23830..25164)
                     /locus_tag="U2I75_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1695643.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            25372..26202
                     /locus_tag="U2I75_000481"
     CDS             25372..26202
                     /locus_tag="U2I75_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEN1695644.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(26446..26728)
                     /locus_tag="U2I75_000482"
                     /pseudo
     CDS             complement(26446..26728)
                     /locus_tag="U2I75_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26886..27080)
                     /locus_tag="U2I75_000483"
     CDS             complement(26886..27080)
                     /locus_tag="U2I75_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695645.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(27585..27770)
                     /locus_tag="U2I75_000484"
     CDS             complement(27585..27770)
                     /locus_tag="U2I75_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695646.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(28620..28961)
                     /locus_tag="U2I75_000485"
     CDS             complement(28620..28961)
                     /locus_tag="U2I75_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1695647.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(28961..29191)
                     /locus_tag="U2I75_000486"
     CDS             complement(28961..29191)
                     /locus_tag="U2I75_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1695648.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            29478..29816
                     /locus_tag="U2I75_000487"
     CDS             29478..29816
                     /locus_tag="U2I75_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695649.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(30021..30260)
                     /locus_tag="U2I75_000488"
     CDS             complement(30021..30260)
                     /locus_tag="U2I75_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN1695650.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(30236..30583)
                     /locus_tag="U2I75_000489"
     CDS             complement(30236..30583)
                     /locus_tag="U2I75_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN1695651.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(31315..31896)
                     /locus_tag="U2I75_000490"
     CDS             complement(31315..31896)
                     /locus_tag="U2I75_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1695652.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(32079..32705)
                     /locus_tag="U2I75_000491"
     CDS             complement(32079..32705)
                     /locus_tag="U2I75_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1695653.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(32727..34058)
                     /locus_tag="U2I75_000492"
     CDS             complement(32727..34058)
                     /locus_tag="U2I75_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN1695654.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(34213..34614)
                     /locus_tag="U2I75_000493"
     CDS             complement(34213..34614)
                     /locus_tag="U2I75_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1695655.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(34700..34906)
                     /locus_tag="U2I75_000494"
     CDS             complement(34700..34906)
                     /locus_tag="U2I75_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695656.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(35001..36503)
                     /locus_tag="U2I75_000495"
     CDS             complement(35001..36503)
                     /locus_tag="U2I75_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN1695657.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(36521..36976)
                     /gene="argR"
                     /locus_tag="U2I75_000496"
     CDS             complement(36521..36976)
                     /gene="argR"
                     /locus_tag="U2I75_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1695658.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(37074..37691)
                     /locus_tag="U2I75_000497"
     CDS             complement(37074..37691)
                     /locus_tag="U2I75_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN1695659.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            37912..39285
                     /locus_tag="U2I75_000498"
     CDS             37912..39285
                     /locus_tag="U2I75_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1695660.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            39360..40472
                     /locus_tag="U2I75_000499"
     CDS             39360..40472
                     /locus_tag="U2I75_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN1695661.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(40544..42181)
                     /locus_tag="U2I75_000500"
     CDS             complement(40544..42181)
                     /locus_tag="U2I75_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN1695662.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(42349..42957)
                     /locus_tag="U2I75_000501"
     CDS             complement(42349..42957)
                     /locus_tag="U2I75_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1695663.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(43341..44492)
                     /gene="queG"
                     /locus_tag="U2I75_000502"
     CDS             complement(43341..44492)
                     /gene="queG"
                     /locus_tag="U2I75_000502"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN1695664.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(44511..45263)
                     /locus_tag="U2I75_000503"
     CDS             complement(44511..45263)
                     /locus_tag="U2I75_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695665.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(45404..46354)
                     /locus_tag="U2I75_000504"
     CDS             complement(45404..46354)
                     /locus_tag="U2I75_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1695666.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(46355..47194)
                     /locus_tag="U2I75_000505"
     CDS             complement(46355..47194)
                     /locus_tag="U2I75_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1695667.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(47191..47937)
                     /locus_tag="U2I75_000506"
     CDS             complement(47191..47937)
                     /locus_tag="U2I75_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1695668.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            48175..49545
                     /locus_tag="U2I75_000507"
     CDS             48175..49545
                     /locus_tag="U2I75_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1695669.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(49600..50496)
                     /locus_tag="U2I75_000508"
     CDS             complement(49600..50496)
                     /locus_tag="U2I75_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1695670.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(50610..50780)
                     /locus_tag="U2I75_000509"
     CDS             complement(50610..50780)
                     /locus_tag="U2I75_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695671.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(50777..51454)
                     /locus_tag="U2I75_000510"
     CDS             complement(50777..51454)
                     /locus_tag="U2I75_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1695672.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(51545..52450)
                     /locus_tag="U2I75_000511"
     CDS             complement(51545..52450)
                     /locus_tag="U2I75_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1695673.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            52625..54079
                     /locus_tag="U2I75_000512"
     CDS             52625..54079
                     /locus_tag="U2I75_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1695674.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            54095..55126
                     /locus_tag="U2I75_000513"
     CDS             54095..55126
                     /locus_tag="U2I75_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1695675.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            55133..56320
                     /locus_tag="U2I75_000514"
     CDS             55133..56320
                     /locus_tag="U2I75_000514"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN1695676.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            56476..57858
                     /locus_tag="U2I75_000515"
     CDS             56476..57858
                     /locus_tag="U2I75_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN1695677.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            57830..58216
                     /locus_tag="U2I75_000516"
     CDS             57830..58216
                     /locus_tag="U2I75_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695678.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(58416..59084)
                     /locus_tag="U2I75_000517"
     CDS             complement(58416..59084)
                     /locus_tag="U2I75_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN1695679.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(59273..60070)
                     /locus_tag="U2I75_000518"
                     /pseudo
     CDS             complement(59273..60070)
                     /locus_tag="U2I75_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(60351..61730)
                     /locus_tag="U2I75_000519"
     CDS             complement(60351..61730)
                     /locus_tag="U2I75_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1695680.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(61905..63074)
                     /locus_tag="U2I75_000520"
     CDS             complement(61905..63074)
                     /locus_tag="U2I75_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1695681.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(63049..64200)
                     /locus_tag="U2I75_000521"
     CDS             complement(63049..64200)
                     /locus_tag="U2I75_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN1695682.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(64160..65521)
                     /locus_tag="U2I75_000522"
     CDS             complement(64160..65521)
                     /locus_tag="U2I75_000522"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN1695683.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(65518..66519)
                     /locus_tag="U2I75_000523"
     CDS             complement(65518..66519)
                     /locus_tag="U2I75_000523"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN1695684.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(66535..67230)
                     /gene="dapD"
                     /locus_tag="U2I75_000524"
     CDS             complement(66535..67230)
                     /gene="dapD"
                     /locus_tag="U2I75_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN1695685.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(67236..67952)
                     /locus_tag="U2I75_000525"
     CDS             complement(67236..67952)
                     /locus_tag="U2I75_000525"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN1695686.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(67949..68830)
                     /locus_tag="U2I75_000526"
     CDS             complement(67949..68830)
                     /locus_tag="U2I75_000526"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN1695687.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(68833..69633)
                     /gene="dapF"
                     /locus_tag="U2I75_000527"
     CDS             complement(68833..69633)
                     /gene="dapF"
                     /locus_tag="U2I75_000527"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN1695688.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(69620..70879)
                     /gene="lysA"
                     /locus_tag="U2I75_000528"
     CDS             complement(69620..70879)
                     /gene="lysA"
                     /locus_tag="U2I75_000528"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN1695689.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            complement(71094..71264)
                     /locus_tag="U2I75_000529"
     CDS             complement(71094..71264)
                     /locus_tag="U2I75_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695690.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            complement(71365..71928)
                     /gene="efp"
                     /locus_tag="U2I75_000530"
     CDS             complement(71365..71928)
                     /gene="efp"
                     /locus_tag="U2I75_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN1695691.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            72356..73273
                     /locus_tag="U2I75_000531"
     CDS             72356..73273
                     /locus_tag="U2I75_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN1695692.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            73286..74431
                     /locus_tag="U2I75_000532"
     CDS             73286..74431
                     /locus_tag="U2I75_000532"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEN1695693.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            74602..75060
                     /locus_tag="U2I75_000533"
     CDS             74602..75060
                     /locus_tag="U2I75_000533"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN1695694.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            75219..75689
                     /locus_tag="U2I75_000534"
     CDS             75219..75689
                     /locus_tag="U2I75_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN1695695.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(75742..75857)
                     /gene="rrf"
                     /locus_tag="U2I75_000535"
     rRNA            complement(75742..75857)
                     /gene="rrf"
                     /locus_tag="U2I75_000535"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        21681 a        15944 c        13428 g        24862 t
ORIGIN      
        1 aaaaagatag aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct
       61 cagtcaattg ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg
      121 acataaacaa ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta
      181 aattctcccg tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta
      241 tccggatgag aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct
      301 ctttctggtt tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt
      361 tgctgggtaa cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga
      421 taagtgactt ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta
      481 gaaaattgag cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata
      541 tcttcaaaga caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc
      601 ttcagttcaa tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata
      661 atcggattat tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct
      721 ggatattccg aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga
      781 ccatccactc tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga
      841 acatggatac ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta
      901 tttagagact cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc
      961 tctcgttcat tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat
     1021 gggcggatct gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca
     1081 tggtagtctg tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta
     1141 aaacttgtac tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca
     1201 agattttcga aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc
     1261 attttgaatc cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa
     1321 gcaactgagt ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg
     1381 tcccaaccgt catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct
     1441 attgtcagtt tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa
     1501 gatgtacagt tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta
     1561 ccgctgattt ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta
     1621 tgatttccag aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct
     1681 cgtaaacccc agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt
     1741 acaggacgct tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc
     1801 ccgttaacgc cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg
     1861 acgattgtct gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt
     1921 ctgcttccgt ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt
     1981 ttacggtagt ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat
     2041 tcttctggcg gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc
     2101 atcagttttg tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc
     2161 ttaagcacct ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta
     2221 aaggacatag tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga
     2281 tcaatcaagc gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca
     2341 acttcgattc cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata
     2401 accgattcat ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct
     2461 tctcctgttt caggatcgta ataatatgcc tctaaaccag tattcgtttt tttcatctct
     2521 acgtccagtt tagtaatcga gttcgtggcc ggaaacagat tcccatgacc gtttccgaat
     2581 atattataag aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt
     2641 tccactgaga cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg
     2701 attgcccgtg tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact
     2761 tcttgtctat ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt
     2821 gaataaaatg cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca
     2881 aatccttctt gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta
     2941 aagttgatgt ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac
     3001 tggaattttc cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg
     3061 gcatacctac tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct
     3121 tcttcagatt gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct
     3181 tcttccgtat ccatatcttc actagttctt ggtatttcct ccattgttga catcccttca
     3241 atgttcacgt gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg
     3301 acagaaatta aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt
     3361 atacgttttt ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt
     3421 attacctaat ttatttttac tgtcaataaa aaacaaaatt ataataagtg tattttataa
     3481 aaagaaaaaa tataacttaa gttgttgata ataaagagat ttattaacgt aaaaagaata
     3541 agttttttta aaataacgta taaaaaagag cataaaacaa agtcgtttgt tttatgctct
     3601 tcttttatta ttttgtgctt tttattttat caaatcactt tgttttttta taaaatttct
     3661 gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt
     3721 gccacggcat ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta
     3781 tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct
     3841 cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa
     3901 taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc
     3961 cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa
     4021 tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc
     4081 tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt
     4141 ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc
     4201 ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt
     4261 cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac
     4321 gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca
     4381 tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt
     4441 cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg
     4501 ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg
     4561 gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt
     4621 ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat
     4681 tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga
     4741 cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa
     4801 ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta
     4861 cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata
     4921 taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata agagatttca
     4981 tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg
     5041 gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat
     5101 aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct
     5161 tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc
     5221 ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact
     5281 gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt
     5341 cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa
     5401 ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg
     5461 cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt
     5521 ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa
     5581 attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg acaccgccag
     5641 gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag
     5701 caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt
     5761 gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct tttttttcat
     5821 ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt agattttctc
     5881 taaatataga aaatccttct ttagtccgat actctttata gtagtccgta tgctaaaata
     5941 agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca tggagggttt
     6001 attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt tctctttgtc
     6061 cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg
     6121 tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact
     6181 gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt
     6241 cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag
     6301 acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta
     6361 ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg
     6421 catctgccga atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc
     6481 aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca
     6541 gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta
     6601 gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag
     6661 gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc
     6721 gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag
     6781 gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca
     6841 cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa
     6901 caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta
     6961 ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca
     7021 cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg
     7081 atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg
     7141 atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc
     7201 cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag
     7261 attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta
     7321 aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg
     7381 aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca
     7441 gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat
     7501 tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag
     7561 atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct
     7621 gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc
     7681 gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca
     7741 acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg
     7801 aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg
     7861 atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat
     7921 gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc
     7981 caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg
     8041 ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca
     8101 tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac
     8161 cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg
     8221 agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa
     8281 acatttttct caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga
     8341 tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca
     8401 caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa
     8461 acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca
     8521 tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg
     8581 ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat
     8641 acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta
     8701 tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca
     8761 ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac
     8821 cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta
     8881 caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta
     8941 atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa
     9001 tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt
     9061 cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg
     9121 aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac
     9181 caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt
     9241 ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca
     9301 gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga
     9361 tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac
     9421 tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt
     9481 ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa
     9541 tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa
     9601 ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa
     9661 cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata
     9721 atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga
     9781 agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat
     9841 tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc
     9901 taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt
     9961 gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat
    10021 aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta
    10081 attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga
    10141 actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc
    10201 aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga
    10261 tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa
    10321 tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt
    10381 tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg
    10441 tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc
    10501 attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc
    10561 agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt
    10621 ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct
    10681 gaatctttaa ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt
    10741 ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc
    10801 catttagcat cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt
    10861 tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat
    10921 tcttggatga ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct
    10981 acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata
    11041 tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg
    11101 acccatgagt caatataatc tttttggtct tgttgattac tgattgctga ttgcattact
    11161 tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc
    11221 ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc
    11281 caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca
    11341 atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc
    11401 acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc
    11461 aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc
    11521 ctacttttgt ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat
    11581 tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca
    11641 gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat
    11701 attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat
    11761 tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac
    11821 ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa
    11881 aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga
    11941 taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca
    12001 aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga
    12061 acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca
    12121 tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc
    12181 cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt
    12241 acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc
    12301 gtttgtctct tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc
    12361 tgttattttt ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa
    12421 gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt
    12481 ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta
    12541 gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct
    12601 tttctctaat tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt
    12661 cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt
    12721 caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg
    12781 tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca
    12841 aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg
    12901 ctccgatcat atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt
    12961 gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct
    13021 gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa
    13081 gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta
    13141 aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta
    13201 ataaaagact gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct
    13261 tcttatttaa aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt
    13321 gcttatactt cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa
    13381 cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg
    13441 cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct
    13501 tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt
    13561 acataacctt ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga
    13621 acctcaaagt atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac
    13681 tcaattctat ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt
    13741 gcgtattcag aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg
    13801 cttgatgcat catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct
    13861 actccttctt cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta
    13921 tggaaatcat ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct
    13981 cctccacgtg catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct
    14041 gtcaaaatcg tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt
    14101 gttaaaatcg tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact
    14161 gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa
    14221 tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga
    14281 tcaatataag aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg
    14341 tcgctttcct ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa
    14401 tgcacatctg tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata
    14461 tcaggcatca tcactggcat cataacaacc attggagacg cgattctgga aatatgagct
    14521 ggcgaataag tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc
    14581 acttggacgg tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt
    14641 gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct
    14701 tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg
    14761 taatatactt ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca
    14821 aaatccgtag aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt
    14881 ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttgactagt
    14941 tttccgttcc cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg
    15001 acaatcattt ttttattcat ttttttctcc ccgttctttt tttattaata aaacagcacc
    15061 taacaataaa agaattccta gtaaagaaat caacgttctt gcttcattcg tttgcgggaa
    15121 tacggaccat tttcctgatt gacctggatt gcttggacca ctcggctctt tcggtttgtc
    15181 cgttgtttct tttacggtct cgttataaac acgtggtgtt gcggattcat aggcagagac
    15241 tggaactttc ccggattcta attcagccat ctcctgtccg ttaactacta catattgtgg
    15301 ttggcctttt gcatccaccc aaaattcagg gatgatgacc tcgattctct tacttgcttc
    15361 atccatctcg taaccttcca caccttttaa ttcttcaaaa taataggtgc cagaaggcaa
    15421 gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa atgaatttgc ttacattttt
    15481 gtcattcaaa ggatcattgt ctgctggttt gatccattga tttttcaagt cattagcagg
    15541 agccatatct aaataatatt tccccccttc tatcatctga tatagagcaa aaacagcacc
    15601 ttctaatggt tttccttttt cagttgttcc tctttgacgt ccatacttga agaagtatgg
    15661 atctcttaga taaccgacat ttttaggata gatatgaatc tcttgcagtt cctcggttga
    15721 ttctgtcggg tgataaattg gcaaaatagc agcaattggg atagctgtct gatcgatatc
    15781 aacatccaat ccaacttctg gatcaatcga ttcttcgaaa atcaaataaa ctgcatcccg
    15841 atcttggttt ttcttaggtg ctttgatacg tgcaatcccg tctcctaatc cgccaaccgt
    15901 atctttccca gttgttaccg aagtccctat ttggacaagc ctttcttctg tgattagctg
    15961 acggatattg tttctgttcg tttcagcgat ttgctggacg aattctttag gagtcatgtt
    16021 ttcagcagct tttttttcat gataataatc ggtcatgtcg taaatggcaa atgtcgctcc
    16081 atttagtggc accgtctgtg aaatgatatc tgctgtttcc cctgcttcct cagagatagc
    16141 caagccatca ttttcatggt attcaaattg atcgttataa tcgatatctc gaatcatccg
    16201 tttatgcaac acgaattcca cgacatcttc ccctgattct tctgccgaca tcgcatgtgg
    16261 aagaaggact gctgcaagcg gtagtaaaac aagtaattga agcatcaaac acaacagctt
    16321 tttcattacc ttgctccctc ctttttagat tgtctgacaa acaaaccata ggctgctcct
    16381 gcaagcgcca tgaagaatgc agcaagcagg atatagaata atcgtccgga tcctcctgta
    16441 gcaggcaacg gattttcagg atcgttcccg acactaagct gtaatctgat tcgatttgtc
    16501 tccaaaagtt catattctaa ctcttgctgt agatcaggcg tctctgcttc ttcgtttgga
    16561 taatacgaaa tctcaacttt tagtgtaccc gcttcttttc ctggaactac ccgatcattt
    16621 tctatgtcag gttcttctcg ataactaatt tctaaaataa agtaacctgg aagttttcta
    16681 tagctgtcag gtgacttcac ttccttgatc cgatagaccc cttccaacaa gtctgaagca
    16741 ttgaataggg aagaatcgtt tggatctggt gccaagcctc cctcgatgat ttcatactct
    16801 cctgcactat tttgtttctc aattgaaaat tctgcgcctt caagacgtcg gctattcata
    16861 ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt catcttctaa ggttacttgt
    16921 agtacaccat cgatgatttc atagccttcc ggcaagggag cagtttcgtc ttcgatgacc
    16981 gtatcttcaa tgataaactt accttcttca ttcagtgcaa actggaacac ttgatcagat
    17041 agccgatacc catttggtgc gacagattcc gagacctcat attcaccaat agacagatgc
    17101 agaggttggt cgtaatttaa atcgacatac ttttctactc ctacggcacg agaatagaag
    17161 tatccgatcc caggcaaacg tttcgattct tctggttcta acggattacc agtgtatgct
    17221 cgatacagcg ggaaggaaga atcccccact ttcctgatat cgaaacctgc accatctaat
    17281 gtatggcctt catggtctaa ttttctaact cggatctccc gataatcaaa cgtattgtaa
    17341 gcagtgaatg ctacccataa acgatcctct tcatctgttg tatacgtgag atattgccaa
    17401 ttttcttcac ctgtttgctt gagttggata gtccctatag cattatcgta aagtcgcaag
    17461 atccatgtgt cattttcatc aggttctttg aaaccttctg ggttctggct ctcaatgatt
    17521 ttgtattctg catagttggt agtttcttca ctatccttat aaacaaatga atctggctct
    17581 ccgattggat gaataacttt ctcaccatcc acgattacat atttcccgtc ttcatctact
    17641 gaatagaacg tgagtctccc tttctcttcc tctacttcgt tcacaccaga aagccctttc
    17701 gctactggat atccgatttc cagtccgtta ccatctgttt cataaagttc aaactctgcg
    17761 ccattgattg gcatattact gccttcaatt agtttattga tattcatgtg gattggcttg
    17821 aattcatttc gcatttccag tttgacatac aactcatcat actctgtgcc ttctatgtta
    17881 ggctcttgta caacagaaag cttgtgatat tctccttcat aataggaatt cggatcttta
    17941 ccttccggtc cgccgactct cgaaccagat gtatattcat ctacaagctc gtagtgacct
    18001 aacatctcat cttcgttggc tgcatatttt ttgatttgga attgatacgt ctcatctact
    18061 aactgataac gtggtggtcc agcgatctct ctgatccaaa atatgtcgct gactgctcca
    18121 gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg ctattccgtc cgtggtagtt
    18181 ttacttgttt cgattggttc ggcttcccca ctctctggat tttggtcata taatgcaaat
    18241 tcgactccag ccaacggctc gccattttga cccttcttgc tgacttggaa attacggcct
    18301 ttatgttcgt aacgaatgga agaagatggg aaatacatcc gtccagtagt gatatcattc
    18361 tcataatcct ccaggttctg aatataggcc atattgttca ctgtgcaaaa atttccgtct
    18421 tgagctcccg catcaagttt tacttggtac ttgatcctga ccatttcccc ttcacctaaa
    18481 gaaatcggag aaaatttata agcttggttt tcccgtccga catacgttgt cggagtcgcc
    18541 actacttctg gtggggcttg tccatgacgt tcttcatccc atatttctgg ctcttggtac
    18601 ttcatgccgt tttctcctgg atctagatgg taaaactgaa tatctgccga tctgccagct
    18661 ccaaatggtg agacattaaa accagtttca tcataaagtg tggcttgctg gatcgttctc
    18721 ttgaaggtag aagagatatc ctccaaggct gtaacaatgt cattcttgtt gttcgcattg
    18781 taatatcgcg gtgttacgcc atcttcttct aaatcagcaa tatttttcaa tacatttcgt
    18841 ccaatagcat ctagtcgttg tctcccgcta acgtttccat cgattcctaa accaatggag
    18901 aagatacttg gcgggttctc ttgatacttg tttttcagcc aatgatgata agcaactgtg
    18961 ttgatcgcag atgacttttc ttgcgaagta ggactgccat tcgtattgtt attagaagaa
    19021 ccatcaccaa cccagtgagt cgctttatta tctccttctt ttacctctcc ataacgatag
    19081 tgtacattta cttcatcttc tggacggtta aagtcagatg cataggtaac tggatgagta
    19141 aaccctgcat tagaagtatt cccagaaaac tcctcaaaat ttctgaacaa aacgtcatta
    19201 tcttcagcaa tcttatcttc catcacagtc cagttgctcc aagctccatt agtgctcgta
    19261 cgataacttg actgaatcgg tgcataactg aaggtcggtg tcccatctcc cacaacgatt
    19321 aggatttttt ctggatttct attttctccg ccattgtctt tatacaatgt ttcatagcca
    19381 tttttaagcc ctagtgtaag cggagttcct ccgattggtg tacgatttaa tgctgtattc
    19441 tcacttcccg tcaaacgatt gatatcattt gtcaatggaa agatgtccga actgatttgc
    19501 tcatggttat tgatagctgt actattatgg taaaaattca ccataccgat tcgaatatcc
    19561 agactcgggt cagagagcag atctttactc atttcgttta ctgcctcaat caacgcagca
    19621 tttttagttt gactattggt cccagcggtt aattcactca tactcgctga cttatctaga
    19681 acaaaaacga tatcgattgg actgatttcc tttttctcgc ccccgatgac atctagatag
    19741 acattgtaaa gggttgttgc atcttcagga tgtagatcag atgctgtgct gcttggcaga
    19801 acaggttcta cccatttttt gatgtacgcc ccgttataat ccaagtaact gttttcaaaa
    19861 ttccgttcat ttccccaaag ctgaacggtt tgagctggaa cgtttctatc ttcttcgcct
    19921 gcagttgatc cattattagt agcatagtcg taatttcgcc agttcgctcc cccgtttgta
    19981 taatcactat catcgatcaa gttctctccg tcatatggcg agcctttgtc tccttgatag
    20041 cgttcattgc tttgtgtcca atttcctaac acttttgtat agtcattcgt tgtaaacttc
    20101 atctattctt tcatcaacca ccacttgaag aggtagttac ccttcgttca catcggggat
    20161 cttttctgtt tcaaaaatca atgtgccggt ttctgcttct tttgaataaa cttttccgac
    20221 ataccattct tgaccatcga tcgtctcgac ttttaggtct tctccatctt catcatagga
    20281 ggatagacct gttccacgaa cgttttttac ggtaccaatc ccggcggctg ccgcatcgat
    20341 tgctaaacgt attctacctt cattggcact ttcctttttt tcaaaagcca gatgccattc
    20401 aagggtttct tctttttcaa tatacttccc ttcaattttt gcatagtcct catctaagaa
    20461 tacctcatcc tcctttgttt ctgttgactc agcagctaca tccagggtcg tcatatgata
    20521 gactgactgg aaatatggtc caaatagcaa actgacagtt actaactgca aaagcttcaa
    20581 ttttaaccct ttcacatcct tatactcctt tcaaaaatat ttgtcttctt tcacaacaat
    20641 caacaaaaca atgcccatct aattataatt taatttaatt ttaattttaa atcaaatgat
    20701 atccttttta gttaaaaaac accaattata caacatttat tattaaaatt aaattataaa
    20761 attttataac gttctcattt aacaaaactt aagttattaa gacaaatgaa taaacaacta
    20821 cgctttcctc gaaacaaaaa aataaagcac aaatgcgcat ttacgcactc atgctttatt
    20881 cgtacaatta attcagataa cggcagcgca taggatgcca ataacacttc ctaaaatagc
    20941 tcctcctaaa atgtcagtag gataatgtaa aagtaaaaaa atcctcgaat aagcgatcaa
    21001 acaagcaaat aaaaaggcta atattcctgc aactggactt aattcgaaca acgcagctac
    21061 tgataaaaag gctagcgctg tatgtcctga aggaaaagaa gaagtcttcg gtttacttgt
    21121 gtaaaggaca agccgacgat ttttttcata aggtcttttc cgtcttgtcg tttttttgat
    21181 aaaaagcaaa ccaacaaaaa aatagatcaa actgccgata agaatggcta gaccatattc
    21241 tctagtatca ggtatcaaga ctaaaacagc agcaaggagc aaccaaataa aaccaccatt
    21301 agagatatta ctgataaatt tcaaaaaatt gatttttcga ctatccgttt tccgttgctt
    21361 ggcgaacttg ttgataatac tatcatcgat tttagcaatc aaacgcaaca tgttattcct
    21421 cctttttaag aaccttttct tgcgtacgat ggataaaaag caggacttga aaataaaaca
    21481 agaaactgaa acctaataaa gcaaaataga ttggtcctgc aaaccagaat gcgacaagaa
    21541 ggatcaacgt gcctgccaaa tagaaaagcg cgcttttgaa atcattaaaa aacaagaaaa
    21601 atgcagtaat ccaagaagct tctttttgtt ccagttgaag caagattttg taactaaaga
    21661 gaagcgcgct taatagaagc aaaaacaacg tagtatacat caatgtatag ccaattcccc
    21721 caccccaata gcggaaaatc tgataatcga tcaatagaag aagaaaataa acacttgcta
    21781 aactatcttg ctttccataa agagagaagt tctcacgaaa ctttttccag aatagtgaac
    21841 cgagcttttt ctcttcattt tttttgtaat gataaatcat atacagaact gttcgacttg
    21901 cagttcctaa tgtgaaaaca ggcaagacgc acaggaacca tagtagctgt aaatggataa
    21961 gacgtggaat ccattcaatg atttctaaga aataatcatt ggctttttta ccaatcgatc
    22021 gattcatcat ccttttgaac ctcctgtcaa atccatccct tcaatgaaat aacgttgtgc
    22081 aaaaaagaac agcaaaatcg tcgggatcaa gacaagtgtt gctcctgcca tcagataatt
    22141 ccattgtgtc gttgcttggt tctgaaaaac ttgcaagcca atctgtaacg tgtatttttc
    22201 ttgatcttgg atatataaca gaggacctaa gaaatcattc caagctgcgt tgaaggaatt
    22261 gatcgcaatg gtaatcaagg caggtttcgt caatggaagc atcaaatgac tccaaatata
    22321 caaatgattc gctccgtcga tttcagcagc ttcgatcaat tcattattga tcgataagta
    22381 aaattgacgc atcagaaata tattgaacgc attgccgaag aacgatggaa cgatcagggg
    22441 caaataagta cctacccagc cgatcttgga gaataataca tattgaggga tcatcgttac
    22501 aaaccccggg atcatcatcg tagaaagaac caaagcaaac atcagctttt tcccaggaaa
    22561 atcaagcttc gcaaatccat aagcaataaa agaatttgaa aggacattac cgatcactac
    22621 tagcacagtg atgaataatg tatttcttgc atagcgccaa aaaggaaagg cggcaatcgt
    22681 atccagatag ttttcccata cgattttttc tgggaaaaag gtcggaggat aacgaacgat
    22741 ttcttccatt ggtttcaatg aagtaaacac catccaaagc aatggaatga tcaaaatgat
    22801 tcctattact gtcatgagga cataacttag tatttttttt gtttgccgat tcacactggt
    22861 ctttttgaaa taatccatca tttgccgtca cctccagaat aatagaccca cttaggtgca
    22921 agcttcaaat tgacaaacgt cagaaccaac acgatcagaa acaggatcat tgacatagcc
    22981 atcgcataac ccatatcaaa agctacgaac gccttgttcc acatatgcag attataaaaa
    23041 agcaaagaat ttgcaggtcc gcctgttcca tttttcgtca tgacataagc ttcttgaaaa
    23101 atttggaaag aaccgatcgt agaggtgatc acatcgtaaa aaataatagg agtgattaat
    23161 ggcaaagtaa tacttttgaa ctttccccaa acgcctgctc catcgatttc tgctgattca
    23221 tataaagatc taggaacatt ttgtaatgta gctaagtaaa gtagcattgc tcctccagaa
    23281 ctccatattt tcatcaaaat caacgcaggt ttcgtccatt gtggatcaaa cagccaagct
    23341 ggtccaggga tatggaacca gcccagaaat gtgttgatca atcctgcaga tggagaaagc
    23401 agctgcatcc ataagatgta gactgctact cctgataaga ctgccggtaa atagaaagct
    23461 gtacggaata cactgattcc tttgattttt tgattgagta ctgtggctaa aaacacactg
    23521 aaaatcgtcg tcagcggtac attgaagatg acgaaataaa cggtgttgta taacgatttc
    23581 caaaataaat catcgtgaac gaacattctt ttgaaatttg ctagaccaat gaaattcatc
    23641 ttggatgtca aattataatc ggtgaaacta ccaataactg aaaaaagcat aggccctagc
    23701 ataaatacca agaacccgat aataaaaggc gagataaata aataggccca tcttgcttct
    23761 tttttctttc ggatagataa acgcttcaaa ttttcttgcc tcctctgtac ctagacagtc
    23821 aaaaatggat tattgatttt gcttgactaa gttttctact gctttttgtg catctgccaa
    23881 tccttctttt ggtgattttg ttcctaaaag aatctggtca atctgttcat tcactaatgt
    23941 cgtgtaatcc ggagcatcta aaggaactgg tgtgatcacc gtatttttaa agttttcgtc
    24001 cgccatttga tagatcattt ttcctttatc cgttaggttg tcattattca ctaaattctc
    24061 attggctgtc ttgctcgcca tgatatcaaa acttttttcg ccgaattttt cttgtacttc
    24121 tggagaagaa agatatttga tgaattcata agaggcctca ggatcttttg ctccgtatgg
    24181 tacttccaag acgaatccgc caccccacga ccaatggcct gaaccttctt ctttttctgg
    24241 gagctgcatc acgccaaaat caaaatcatc cggtgcattt tccatcaaac tagagtaata
    24301 attgatattt tgggcacgca tggctactaa tccggaaata aatggatcag atacaccaga
    24361 cccaaattcg gcttctaatt tattgatcgt atttttccca taataatctt gccagtcttg
    24421 gatccactcc aaggcttcca ctttattttt agtatcaatc ttgacttttt catctttgtc
    24481 aaaccaacta gtcccgttat ctgcgtttag cgcccataca tcagccccta aattccataa
    24541 tggataaaaa ccgatgcgtg taaattcttt tccttctttt ttatctagtt tacgtgctgt
    24601 ttcttctaat tcatcccaag ttgtcggtag atcttgttca ctgattccag cttctttaaa
    24661 taacttttta ttataaaaaa tgacttgtgt atctgtattg aatgggatcg cataaggatc
    24721 accttcatat tctactgtat cccaaagctg cggataaaaa tccttagaag tgtctttttc
    24781 tacatatttg gagagattag ttgcttgctg tgcttctgct ctttgggcaa cggaatttat
    24841 gtcctgtaca ataacatcgg gtgcatttcc agctgttatt gcagataaag atttagtcca
    24901 gatgtcaccc catggctgat acttataatt tacatggatc tcgttttgtg attgattgaa
    24961 atcctcaatg atttcttcga tagcttcttt tcgtgcaccg gatccccaaa aggaccagaa
    25021 atcaatattt tttcttccat cacttgtttt ttcagaagaa tttgctgcac ttgaactggc
    25081 atcgcttgca ttacttgatc cgcatcctcc taataaaaac aatgctgaac ttgccagcaa
    25141 ccctgtaaac aataattttt tcatatttta cctccaaatt ttaatgtaac cgatgccggt
    25201 tttaatataa cgatatcatt ttgttctgaa aataacaatt tataaggtat gattctaaaa
    25261 aaattattaa tgataccaat actttatata attcgcgagc gctttctttg aattttttaa
    25321 tctttcgtag cagaatgaga atgtattaaa aatagattag gaggaatttt gatgtttaga
    25381 caccagaaag aattgcagtt tgaagcaaaa ccagatcgtc cgaatccatt aatcgcgaaa
    25441 tatctgcagg agctgatcgg cggccaatat ggcgagatga gtgtagctat gcagtacctg
    25501 ttccaaggct ggagctgccg aggagaagaa aaatataaag atatgctgat ggacattgca
    25561 acagaagaaa tcggccacgt ggaaatgttg gcgacaatgg ttgctcgttt attagagggt
    25621 gcgcctgttg aagatcaaga agcagcgatg caaaaagatc caacaatcgg tgccattatc
    25681 tctggaatga atccacagca cgcgatcgta gctggcttag gacctcgtcc tgctgacagc
    25741 gtgggcaatc catggagcgg tgcctatatc attgcgagtg gcaacttgtt agcagacttt
    25801 agagctaatt taaatgccga atcacaagga agacttcaag tagcacgtat ttacgaaatg
    25861 atcgatgatc gtggcgtaaa agatatgctg tctgttctcc ttgcaagaga tagctatcat
    25921 caaaatctat gggaatgtgc agtgaaagaa ttagaagaaa aagaaggttc aatcttagtc
    25981 ccaagtactt tcccgcgtga caaagaacga aaagacattg cttatgactt ctataacttc
    26041 tcagagggag accaaagtag tgaaggttct tgggctagtg gcaaagcact ggatggcgaa
    26101 ggcgaatatc aatggttgaa agaacctaag atcgaaggcg ctgcaccaaa attagaacca
    26161 gtaacaccaa aaatgtatgg gacacctcca ttcaagaaat aaatatctac ccgtagaaac
    26221 acccgttttt tgttccacac gctttgtgag caaaactgac taaagtcatt aatcataacc
    26281 acccttcaaa agggtggttt tctctagagc tataagctca aatcaccggc cagcgcctaa
    26341 agacgctggc tttcatattt tatgttcaag ccatattgcc tttgccagtt ttgcccacct
    26401 cttaaaaggt ttatgtacta ctccttagcc cttaaaagga tcgtcttact ctcgtacact
    26461 cagcttatct aacgcactat catgtttttc ttgttcttga atgtactttt tgatggtcgc
    26521 ttcattttgt cctacttttg tcacatagta tccttccacc caaaagtgtc gatacccaaa
    26581 tttatatttt agatttgcac aatctcttga tgatctcctg aatattggct cgatattgat
    26641 tataaattat ttttcttcta tactttggtg tgaatacaat gtggtattta cacatctact
    26701 ttgtacgggc taaactattt ccctttattg ctccacccgc atagcaagtg gttttgtatt
    26761 tcaatcgctc ggcgcgctca attaggtcta aagacaataa atctaaaaac ccgctgcttc
    26821 actgacagaa acaacgggtt ttcagaaaaa aatgaaatga aaaagatctc ttacggcaat
    26881 ctattttatt tttttgattt tttataaatg gaaaatgctt caagaagtcc aagaaataaa
    26941 gccgtataaa aacaagaacc tatgaaatct ttatttatta gatattcaac agaaatcccg
    27001 ataacactag ccaacaatat atttatcaga agtgtaaaat aaggatgctt tttatagaat
    27061 agaactagtg ttgttttcat agactctcct ttcttcatat ttccaattaa tcacaaattc
    27121 acggcattac tagaaaaatt aaaggggcgc agaagtggaa aaatactctt cgactttccc
    27181 tacaaaccag gccatcttgt gtttaataac tataccaaat ctaaggtacc tctaaatcta
    27241 aattccattt gtggcggtga cctccaattt ttaactcttc tatctcccat ccagtataca
    27301 taaccttgat acttcgacca cttatatatc gaacacacaa atacctatcc attgcaggag
    27361 tactacctaa cttagttgct acttgcatct aaataacagc tgaaaagata ggacttactc
    27421 ttagtaaagt atctgaacta ttaattttta aactatcatt tttatcctca actacatgca
    27481 catctgttgc aacttcactt tctgtctcat ccagataaat gattaaggaa tcgggaattg
    27541 caatatcttc agctgacgct ggaatagttg agctaaatta gtcatcatat ctttattata
    27601 aaataaccaa cagcaaaaaa tacaatgaaa aacaaacttg gaactaatgt atcattaata
    27661 aatgttgaaa aattcagcaa ctcattactg tatgatttta gcagcccaac caacatatac
    27721 cctataaaag ctaataagat ggttaggaat cctttagaat ttttactcat tcatagacac
    27781 ttccttaaat aacttttatt atagaaggta ttgtaaataa taatattgac aaaaagtgac
    27841 tcaaaataaa aaaaaactta cattatactg gaatgaactg tattgatagt gtacctaaaa
    27901 gctctactga cataagatcg tgtaatcggt aaagtaattt tattaattat acgggatgta
    27961 atcaattaga gaataaacta atcataaaag gtacttttac attcataatt gtcgctttga
    28021 gattctactt tggttcacta tctgacccaa ttgcacattt tcgatcatta caagtattgg
    28081 tgtcgttaga acaactaaat aaaaactagc ggtagatttt tcagaatcta tgctaatttc
    28141 tgtatggatg ttccagtgat ggcaaatttc tcttttcatt gtttttcttg tcgcatgata
    28201 tgcctttttg tgttattcag ttaaactata gaaattcttt cctagacaga tcttgatccc
    28261 aaattttacg tttttgagca tcgatatagt ttaaaatgac attactcata tctaaagagt
    28321 ttagattttt taggactaaa actgtcagtt gttctttttc ttctttctgt taaagtattg
    28381 tcgcgacttt tcgtatacgt taagatatgt cgtgaaaaaa caagttttgt gacacaaaaa
    28441 aacgaatgac ttgaggaaat cacctcaatc attcgtttaa aatgctatat attctttagt
    28501 ttaagatacg gaaattaatg ccatcgacca cactagtaga atgaacgaca tttacttggc
    28561 ctttttttaa tttcgttatg tttagcatat aaaaatatta tagagtttac ttacaatgct
    28621 taaaacatat actgtataat ctgatcaatt ttagccatgt cttgcaatgg taagttttct
    28681 atttttttga gttttcgttc cttaaaatca agagatttta attgtgtaat aattacttgt
    28741 ccttttatat ctagctcttc aggtaatgag taacgtgtcg gtaaattctt tatagtggaa
    28801 gtaattggac atatgaccga aaacattgtt ttacggttaa attcataacg agagacgact
    28861 agtcctggtc gtcttttttg aatttctttt cctacggagg gatcaaaatc aatccataca
    28921 atatcacctt ttttaggaat ataaattctt tcttctgaca ctataaaatt tctctccctt
    28981 caggagttaa atcttcccat tcgtctttct cataatactc ggcctcttcc ccaccactaa
    29041 atggatcttc aatttttgga actaacgtaa ttgttccatc atcagaatac acaacgatat
    29101 attcttggtt ttctgaaggt ttctttccgt tatcagaagg taaagttacc actacagaac
    29161 ttccttgtaa tctagatttt gtaacaagca ttatcatcac tcccttaata aaagtatact
    29221 atataacact aagtaacgca aaattcccct ttgtaaacta tctagaaaat tgttttgttt
    29281 aaagtatatc ttattggata taaatagaaa taaattgtag ccagttcatt ttttctttaa
    29341 ctaaaaaaat aatccaagaa acattgaacc tatggatcca aaagctaccc aaatactata
    29401 gctaaccccc cgctaccttc aattttgcag ataaaacgct ttgggagaaa atttcaaaga
    29461 caacaggcca tcctatcatg cccgattata aacaaaaagc tggctattcc ttttgctctg
    29521 acaaatttca attgggaaag ttcacgaaac tagcaggtga agaagtttca actgttcgga
    29581 tcaaagaatg cccgcttcaa atagaaacat tggttcaacg aatttcaact agacaagact
    29641 ttgctattgt agaacgtgag atcgtagcta tttttgtaga agaagggatt ttacatgatc
    29701 caactcatat caatgtggat aaatggcaac ctcttatcta taaattcaga gaatatacaa
    29761 ctgccaacca gtcgttaact ttgaatttta gattccaaga attcaaagag tcataatgat
    29821 aaacatcgtt aggtataaat aaaaaagact cgcagcacga gtaaaaaaca aatagaagct
    29881 atacattaga aaggtcgact gtgaccctat agatgcagta tatgcagctt atgagtattt
    29941 cttctgttct ctttgttttt gggatctgtg ctgaagtctt tttcgttttt tgccgctctt
    30001 tctttgaaaa atcgattatg ttataccttc tccattacta attcaggcat ctgctgagta
    30061 atgatttttc gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc ttcagtcaag
    30121 acaagcggca ttcggtcatg gattggtgcg actgtgcggt taggttcagt agtcattatg
    30181 attgattctg ccattttttc atcctgatgg ctacgataaa aaccgccaat atacactatt
    30241 ttttccttat ttgaaaacat aaatttgtct ttttcttcat tccactcatt gggaaaacac
    30301 aacgattttg caaaaatggt tgatgaaagg tctttttttc ttctactgtc tctgcacggg
    30361 cattaatcat caattgtcct tttttaaatc cggtaaatcc ccacttcatg atttccggag
    30421 tgaggatgtt ttcttcctct tttcttaacg tcacaacaag atttgacgga aaaacttctc
    30481 cagctgctaa ttctgctgcc tgcttcggtt gtttcgtctg ggcttctcga taataattct
    30541 gaaggagctc tgactctaga tcaaaaaaat aacgaccaca catcctaatc cctcccaatc
    30601 tttttttcta tcatacactg caacaagaag ttcgcaccaa caaaacgctt tcaatttttg
    30661 tagaactaaa aaagttgtga caagtgacaa actctccagt gaatggtatt ctcctgccaa
    30721 ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgcttttt gaaattgttc
    30781 cttgtgactc ggagtgaaac ggcaggctta cgacctctgg gtaaacggtg ttcaaaagcc
    30841 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagctatttt cacaaaattt
    30901 ttcttaatac tcgaaactaa gcgacttttt cacaacctct tccttaaacg gtgttcaaaa
    30961 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagcagcta tttttatttg
    31021 gctgttctta ttacttgatg cagggcagct ttttcacaac ctctccacta aacgatgttc
    31081 aatcagctaa ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgccg
    31141 tttgtttctc taatactcag gtcaaagcgc tgatttcaca acctctaata cacggtgttc
    31201 cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaatttgaga agcaattttc
    31261 gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcta attattaaag
    31321 aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc taagtagtga
    31381 tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa tcaacgcttt
    31441 ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca ggatattttg
    31501 tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt attcttcatc
    31561 cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag tgatctgtgt
    31621 ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg gctgttttcc
    31681 agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg catggatcac
    31741 cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt ttcctgtatc
    31801 agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat aaataagatc
    31861 tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt ttcatttctc
    31921 atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt aaaaaagact
    31981 gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc tagatgttca
    32041 ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg ctctaatact
    32101 tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc tgcatccatt
    32161 gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa tccactctct
    32221 accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc aagaattact
    32281 gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg tccaaagatt
    32341 ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc ttcgataaaa
    32401 tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa catctgctgc
    32461 tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg tgggtaaaaa
    32521 taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac catttcaatc
    32581 atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa gccaccgcct
    32641 gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt tttattattg
    32701 aacatggtgg attctccatt cgttatttat tttaatacat catttagagc ttcactcatt
    32761 gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc attcatagct
    32821 agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc ccctagaatc
    32881 aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc taatacttgt
    32941 gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc ttgtgctttg
    33001 gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac actgtaaggc
    33061 acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc cataatgatt
    33121 cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc
    33181 caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc gcgatctgtt
    33241 agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct tccagttgca
    33301 gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa ggtgacggca
    33361 gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa ttcgattccc
    33421 gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg tggaagaaat
    33481 tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat tgatgcaaat
    33541 tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg ttcctgatcc
    33601 atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat tggtaaaatg
    33661 attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac tttttcacca
    33721 ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc catcactgtc
    33781 ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc cgtcaatttt
    33841 tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa aatcaatgat
    33901 ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt tgacttttca
    33961 acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt accgcccttt
    34021 ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct tcttttttac
    34081 ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa tcatctgctt
    34141 tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc tggttcgatt
    34201 tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag ttgctttgga
    34261 gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt cgctgatgcc
    34321 atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta aattataaag
    34381 agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga tcatatgttg
    34441 taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg gatttgttcc
    34501 ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg gtgcctcgac
    34561 ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt tcattgtact
    34621 gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac aagtaaggcg
    34681 ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact atttttccaa
    34741 aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa
    34801 tcttttctac ataatctttt atatgattga ttttaagata catatatacc agaaagttga
    34861 ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt tcctcctttt
    34921 gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt ccaagcaact
    34981 agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga aaaatgtccg
    35041 aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc attttctatc
    35101 tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc gtttccgtat
    35161 ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt catccctttt
    35221 tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc gcgtggttca
    35281 tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa ctctttgatt
    35341 tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag gatctttgcg
    35401 atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg tccgcttgtt
    35461 tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt attgattaca
    35521 tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat gaatctttgt
    35581 agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc ttttaatacg
    35641 attgtactta atactgcgat caaattattt cgattgatca ctgttccaga agcactgaca
    35701 actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa
    35761 acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat atgattagga
    35821 aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa gctattcaat
    35881 actttttcag caaagaatcc accagcacca tttccggcat caacaatgat tttccaacca
    35941 tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac taaatcttgc
    36001 gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct ttttattttt
    36061 tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc tgaggtaaag
    36121 aattttattc cattataata atagggaaga tggctagcag tcagcataac cgcagcatcg
    36181 cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc taattcgaaa
    36241 tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt cagtgctggt
    36301 ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg atcggttgat
    36361 ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac catgtctgtc
    36421 gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc cgatccattt
    36481 tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc attttttcca
    36541 gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg aatgtatcta
    36601 caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa ttcgttacca
    36661 catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac tggatacgca
    36721 aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca gatggctggt
    36781 taaataatgt atagcgtact attttgtttt cattttgact ttttactaac ttcatttctc
    36841 gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa
    36901 gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc
    36961 gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag ataagtaata
    37021 tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg ttgctaacct
    37081 ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt tcttggatcg
    37141 ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt tgcgatgaat
    37201 gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg taagatttgg
    37261 gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc ttccggaagt
    37321 ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg
    37381 atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa atacttttct
    37441 tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc aggaatcagc
    37501 caattttttt taggcgacat gcgtattgca tagctgactg catatagaca ggctactctt
    37561 cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa tgatttttta
    37621 ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt acgccattca
    37681 attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat catacctctt
    37741 tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag aataagtgaa
    37801 gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg tttctatttt
    37861 tataatagaa atcactctct ttttttacga aattttttag aggtgatgta aatgacaaaa
    37921 caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc ttttgcgggc
    37981 gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca gtttgattta
    38041 acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc gatcattgtt
    38101 ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt agcttcaaat
    38161 ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat ggtcctattg
    38221 tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat gttccatatt
    38281 atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg tactttgaca
    38341 acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc acattttaca
    38401 tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat tatcggctgg
    38461 tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat aagcgtttta
    38521 ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg aacagtttcc
    38581 agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta tcgaagtaaa
    38641 aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt ccgcttgttt
    38701 ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt cttgccgaat
    38761 ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat gcttcccggc
    38821 gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca atatggtccg
    38881 aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc attattccta
    38941 ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta tatgatcggt
    39001 atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa tgaagaacta
    39061 caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc tgtggctact
    39121 tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa ctatgaagtg
    39181 tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc
    39241 tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc atatacggca
    39301 ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta
    39361 tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc agttacttag
    39421 atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca actaaagcta
    39481 gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg
    39541 ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt gatcaaacga
    39601 tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct gaattggctg
    39661 ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa tggctttcac
    39721 ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg cgtatcagtg
    39781 gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt ttagcacaaa
    39841 aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat tcttggctca
    39901 tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct ggcccggcac
    39961 ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa aaagggcagc
    40021 tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct atgcttgacg
    40081 gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct acccatgaaa
    40141 acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg actaagagta
    40201 cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag ttccgtgtag
    40261 gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca gatgatccaa
    40321 gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct tatatcggct
    40381 atctattagc caacttctta aatactggtc attggcctac ggaagattat caaaaggaca
    40441 tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag taaggatccc
    40501 gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc aatttctgaa
    40561 tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct atctgagctt
    40621 caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag gacgtatcct
    40681 caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat ggaccaaacg
    40741 acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt
    40801 tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt tgctgtttca
    40861 aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg aaaggattag
    40921 caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt tccgcgtctg
    40981 atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca ttgatcttga
    41041 taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc ttttcgctaa
    41101 gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca tttccgtttc
    41161 gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg cccacattct
    41221 gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt gttttatcaa
    41281 agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg aaagcatcgt
    41341 catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc gttgattttc
    41401 cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta gcatattgtt
    41461 tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa gcaagcgtca
    41521 aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct gtattatgga
    41581 gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg ggatcacaaa
    41641 agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct aagatcttcg
    41701 aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat ccttctggaa
    41761 tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact tttgtatggt
    41821 aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg tatttgcttt
    41881 gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc acaatgcttc
    41941 catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca gcatgtgaga
    42001 ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa gctgtcttcc
    42061 tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac
    42121 tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct tttgtactca
    42181 tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt attcgagttt
    42241 ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa aaaaaccgag
    42301 aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt agtaccagcc
    42361 gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg ttacatattg
    42421 ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg aagcatctaa
    42481 ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg attctttttg
    42541 tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg gggcagcagg
    42601 agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt
    42661 tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat catttgaagt
    42721 tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg aatttttttc
    42781 agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct tagataatga
    42841 atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc ctacaaacat
    42901 tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg aattcatatt
    42961 aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat catttagaaa
    43021 tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta tttaatattt
    43081 gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt gtataagcaa
    43141 agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc tgtaacattc
    43201 actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa ttctcttaaa
    43261 aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga caaaaacttt
    43321 gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg ggcatgttcc
    43381 aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa tacatcaagc
    43441 catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt tcctctgatg
    43501 acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg atcacctaga
    43561 ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca tgaaccagct
    43621 aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg ctttaatttt
    43681 ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc ttttccttga
    43741 ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg cattttttta
    43801 cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca tcgctgggca
    43861 ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca gcgcatacag
    43921 tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt aacgatctca
    43981 ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg tccaataaat
    44041 cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc tacttgcgga
    44101 gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat gaattcgatc
    44161 aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga agcacgagca
    44221 aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg atacgcaagt
    44281 gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag acgctcatcg
    44341 atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc caacgattct
    44401 tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc taatcgtttg
    44461 ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg ttaacggctg
    44521 tataacccat aatatagttt ttcttttttc agctggacaa actggatctg ctgatcggca
    44581 atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata atttgttgaa
    44641 attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg ttttgtactt
    44701 aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc gcgggaatac
    44761 aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg gattttccca
    44821 ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct gttggcaata
    44881 aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc tttgatgact
    44941 gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac taatacgcca
    45001 ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt ttcatctaaa
    45061 ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg ttctgttgtc
    45121 tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg aaggtcttga
    45181 tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt aatcgtatat
    45241 ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac tataacggaa
    45301 tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc atatctctta
    45361 taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt tcttttgttt
    45421 gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag taagaatctc
    45481 cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt ttcaaacccg
    45541 tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg aataaaaccg
    45601 gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc gtaccttgac
    45661 tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt gaaaaatact
    45721 tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa gcaaaacttt
    45781 ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca ttttttttat
    45841 agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc tccacatatt
    45901 ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt tcttcaccgc
    45961 tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt ccttcttctt
    46021 ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga ttcaaaccgt
    46081 tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt acttcatatt
    46141 catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct gttccgacct
    46201 cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt tctttacttc
    46261 cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag aaactaactg
    46321 ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc tgaaataaaa
    46381 ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat gctaacacga
    46441 tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca actgcactta
    46501 gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt agcaagtaag
    46561 ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact gtctgtaaac
    46621 tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc gatgtgttca
    46681 gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct ttataaaaga
    46741 aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa tcgctttccc
    46801 taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc tgggcatatg
    46861 aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc gctgtatcat
    46921 ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc atcccaaaag
    46981 cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca cctggaagaa
    47041 ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata aaagaaccga
    47101 tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc aggaattgat
    47161 aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt ttactaaatg
    47221 tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt cttgtactgg
    47281 accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt cttctacttt
    47341 atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt cctgcaagat
    47401 gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat ctaaaaagat
    47461 ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct gtccacccga
    47521 tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca aagcttgtct
    47581 tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg gatatgttcc
    47641 taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac ttcgctgttc
    47701 cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg tgactttacc
    47761 agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc ctgcaccgtt
    47821 tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag ttttcaaaac
    47881 agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac gttccaaatc
    47941 ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac taaaatggaa
    48001 agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca ttttatgaaa
    48061 attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga aaaacatgtg
    48121 ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt ttgtatggaa
    48181 gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc actaggtgga
    48241 acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac agggccctca
    48301 gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg tgctttaggc
    48361 gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag cgagtacatc
    48421 catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat tgtcgtagga
    48481 ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga tttgccggca
    48541 attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat ttctgtcaag
    48601 gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat cattttgatg
    48661 atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca ggcagtaggg
    48721 atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg atttttcttt
    48781 gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat gactgctggt
    48841 gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat cggtcgaatc
    48901 ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg gaatcaattg
    48961 agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac atttgctgca
    49021 ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc agggattttc
    49081 agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa aaaatttctg
    49141 aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg tggaatctta
    49201 attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga aaatattttc
    49261 gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt gattttgatc
    49321 agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc ttttaaaatg
    49381 ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt attgattttc
    49441 atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt cttgctttac
    49501 atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa gaacgaagct
    49561 tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt ttagcgtctc
    49621 accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga tcgtttctac
    49681 tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa ctacggttgt
    49741 cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg tgtcttgtgt
    49801 tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat cagaagatgt
    49861 tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca aataaatatc
    49921 ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca aagtatctaa
    49981 gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc tgatcccttc
    50041 gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata aaccagctat
    50101 tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag gcgtcttttc
    50161 ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca aaccatttgc
    50221 tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac ttgtttgttt
    50281 cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt ctgacgaatt
    50341 ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca cggcaactgc
    50401 tgccccaatc atgatcatcc gcttacgagc tgcttgtttt tgctgtcttc ttcgggtcat
    50461 ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca atttttatgt
    50521 ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc gctgtgcaca
    50581 tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca agattgtccg
    50641 tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag tttctttatt
    50701 tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc taattgttgg
    50761 atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta aaggtcaagt
    50821 cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt ttttcagatt
    50881 cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca atccgctgag
    50941 tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc ttctcccaac
    51001 gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga taggtcatat
    51061 ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta tattcttctt
    51121 ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt cctagttctt
    51181 tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt tttaaatgag
    51241 caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc tgggccaaga
    51301 gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc aaatgatgcc
    51361 aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa gattcttcga
    51421 tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt tagtcttcgc
    51481 tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat tttaaccaga
    51541 ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc aaggtatcca
    51601 aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta gccgtttctc
    51661 cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct acttgatagg
    51721 caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga ttgatccgta
    51781 ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact cgcgacaaat
    51841 ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt tcagcataat
    51901 gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt tgtacaccac
    51961 cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt aatgctgtta
    52021 actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa ataatgtcga
    52081 tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa agcgactgcc
    52141 gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc cacggatctt
    52201 ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga tcaccttttt
    52261 tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc acgacatgca
    52321 ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt ttagagaaac
    52381 cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt aaaaaaatcg
    52441 attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa gttaaataaa
    52501 agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc attaaaagat
    52561 ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga gaggaataca
    52621 gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt ttggacacga
    52681 cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc agatgttcgc
    52741 aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct tagttgtagg
    52801 tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg ataatactcg
    52861 tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta gttccgtact
    52921 cttggtagca atctttacca atttctttgg cttagctact cataatacga ctttatttat
    52981 catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta aggatatcgc
    53041 tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa aatttgctac
    53101 gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg ttgtccctat
    53161 cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt caggctggtt
    53221 ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca ctgccttggg
    53281 cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga tcaaagatgt
    53341 gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt atatcgctta
    53401 tgcgattgcc aatgtagcaa caactgccgt attattctac ttcttcaaat atgtacttgg
    53461 ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt tagtagctgt
    53521 tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc ttggagggat
    53581 cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt tgccaatcgt
    53641 tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat ccgctttgat
    53701 gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta acgaagctgt
    53761 tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta acgggatcgt
    53821 agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg atatcactgc
    53881 tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt tgatgatttt
    53941 ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg cacaaatcgt
    54001 tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac ttcacgatgc
    54061 agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa aagattttgg
    54121 ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt ctatctctga
    54181 tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt tgggatttga
    54241 caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg gtcgcaccgc
    54301 agggagaatc gaaaatggcc acttttctct caatggaaaa acttaccaat tagccacaga
    54361 tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa aaaaatggtc
    54421 ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta caagtcccga
    54481 cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt tgacaaaaga
    54541 taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat tcaatccgac
    54601 aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc aacatgagct
    54661 ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta taggggtaaa
    54721 agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag cgtccgtttt
    54781 tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat ttttcttaaa
    54841 agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa gaatccaagt
    54901 agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg gcacagaaac
    54961 accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg agactcaaac
    55021 agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag ccggatcagt
    55081 atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca tcatggaaat
    55141 caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa ctacacaata
    55201 cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg gaggccatgt
    55261 cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag acgataataa
    55321 aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta ctttagatga
    55381 gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg tccttaaatt
    55441 aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag gaacaatccc
    55501 taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg ttgtattaga
    55561 agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta aaaaagtaga
    55621 aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg gcttcggtga
    55681 gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg ttccagtgaa
    55741 actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat tggcagattc
    55801 aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc aaaaaacttt
    55861 gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta ttgaccaaat
    55921 cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc agcggacttt
    55981 gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac tgttaaatga
    56041 ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag atacactagt
    56101 agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag ctggatttgg
    56161 tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa acaatgtcta
    56221 tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac acattggaaa
    56281 tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc aacgatctca
    56341 gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag ccttttgctc
    56401 gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc taattgtgtt
    56461 gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga aaaagaacct
    56521 tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga gtgcgttgaa
    56581 aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc cgattcgaca
    56641 gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa agcttacggc
    56701 atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga tttatatatt
    56761 gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc tatctataaa
    56821 aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt ccttgatgta
    56881 acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa actgatccat
    56941 aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga taacccgcta
    57001 cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt tgctgaatgg
    57061 cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga cttttgggga
    57121 atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt ttatgattta
    57181 gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac tcagctttat
    57241 gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc aaaatcaaaa
    57301 agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga aatcgagatc
    57361 gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc agcaaaaaca
    57421 caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa tggcaagacc
    57481 gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct agcagatgct
    57541 ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt aggcgtgaac
    57601 tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga tcctgaaaag
    57661 cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa atacggcttt
    57721 caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc acgtagttct
    57781 ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga tgagacggac
    57841 agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat taaaatgggg
    57901 gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag aagaagcacg
    57961 caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta tactgacaga
    58021 agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg aatttggaag
    58081 actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt actttatgat
    58141 tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg aaacaaaaaa
    58201 atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat aagaaaggat
    58261 ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg aagaagcaac
    58321 ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag ttttgagtaa
    58381 caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg gtaagattca
    58441 tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca ttcaacataa
    58501 atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag ccatgcttca
    58561 gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac aagcaggatc
    58621 gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc attctctagg
    58681 ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat tgctcgcaac
    58741 tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc gtctttgatt
    58801 agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg gacgatacgt
    58861 tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg ccctacatgg
    58921 acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt atagactaaa
    58981 tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac taataataaa
    59041 ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc tccccctaac
    59101 tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat acaaggaaga
    59161 aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa aagttagact
    59221 tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat ttttacacaa
    59281 ccgttacggt tgttttacga aattggacga gactttgcca gtttaatttc tgttttatcc
    59341 gttcattatt gtagtaatag atatagctat cgatggctga ttttaattcc tcgaaacagt
    59401 tataaacttt cccgtgaaaa atctcttgtt ttaataagcc aaaaaaattc tccataagtg
    59461 aattatctaa gcagttccct ttccgagaca tgctttgaaa gattttgtac tccttcaatt
    59521 tgtttctata agctttcatt tgatacgccc aaccttgatc tgagtgaaac gtacgacgat
    59581 agggacaatc gttagtggtc tggatagctt cctccaaacc ttccattacc gctaatgcat
    59641 tgggcttttc agagatacga taggataaaa tctcactatt gaacatatcc ataaaaggat
    59701 ctagatacaa tttcttttga cggataatgc ctttggcatc cacttcatgg tatttaaatt
    59761 cagttgtatc cgtcgtgatt ttctgatggc agatactggt gtagaaacga cgatggatcc
    59821 gatttttggc tactgctcca acttttcctc gataagaatt gtagcgacgt gatttacgcg
    59881 tgtatgccgt tacttttatt cctagcttct tgatcaagcg ctgaactttc tttttattga
    59941 tagagaaacc gcggtttctt agttcattgg tcatccgtaa acagccataa tccttgtgct
    60001 tctcccgaat tttaagcatt tccgcttcaa tctcttgatc tggattttta cggtccaatc
    60061 gcctttgcca tccaaataat catgaacgat ctttaattta agttcaaaag tatatttaac
    60121 cataaaaaat accgacctcc aaaagttaga ttttttggtc taacttttgg gggtcggtac
    60181 acaaatcgtg tcacagtccc ttttttgaaa ataaacggtg ttcaaaagct tgcatctgcg
    60241 gtaataagtc cagccaagtt aaaaacataa gaagctgttt ttacttggct gttcttatta
    60301 cttgatgcaa ggcagctttt tcacaacctc ttttcaaata tttcgttttc ttattctaca
    60361 aatggttctt ctcctaacgc ttgttgagcg gctagattca acatgctcca ttgtcggtca
    60421 aagcctggct ggaagaagaa gtcagcttct gctagttctt ctaaagtcag tttgttttgg
    60481 atcgctagtg ccaatacatt tccttgacct gtaacatcat aggtagataa tactgctcct
    60541 cctagcaatt gatgggtcat tggatccgag aataattgga tggacacttt tggatttcct
    60601 tgttcttctg gtacatattt tggtcgtaac gtatcttcat aataagacac tcgtacatct
    60661 actcctgctt ttgctgctgt aaagctattc aatccgcttg ctgcaaaatg atagtcaaag
    60721 acacttaatg cagaggaacc tacgactccg ccgaacggtt tgtttggtac ttcttcaaaa
    60781 agatgtttta ctacataacg tgcttctcta cgtgcaacag tcgctaaagc gatcggcatt
    60841 tttttgcgtg ctgggataga ataagcaagt gtagcatctc ctactgcata cacatctgga
    60901 agattcgttt gcaagtattc atttgtttgg atccagccac gttcatctaa gtcgacgatc
    60961 ccttttaacc attctgtatt tgccttcaca ccggcagctt gaatgactag atctgctggg
    61021 atttcttttt cacttgtttt gattgcctca acttttccgt taccagtaaa agcttcgatt
    61081 tttgcatttg tcaaaacttc aataccgttt tcatttaggt gttgttctaa aatatctgtc
    61141 atctcagcat ctaaataagt gcctaaaggt ctatcgatca catcaagcaa tacgacttct
    61201 ttcccagctt ttcggcttgc ttctgctgct tcgatcccaa tgtaacccgc acctacgaca
    61261 gcgatttttt taatagccgg atcatttaat ttttctttga ttttggttgc ccaattgtat
    61321 ccacgcatca agtagacatt ttctagatct gctcctggta ctggcagatt gtttggtttg
    61381 acaccagaac tcaaaatcaa tttgtcataa ggatattctt ttgtttcgtt atctgatgcg
    61441 ctagtaaccg ttactgtttt tttatcggca tcgatcgttt ttacttcgtg attatttaaa
    61501 atatgaacat tggattgcgg aaagttttct ggtctgaaat tacgtacatc atttacatct
    61561 gttaccttgt cttctaaata cagctccata ccacatgaca tgaaggaaat atagtctcct
    61621 gcttcaaata gcgtgatatc tacattttca ccgtatcgtg tcaatagttc caagatcgat
    61681 tgatgccctc catgagatgc acctacaatg acaactttga ttttttccat tgatgaaacc
    61741 tccttgaata ttcttttaaa atcattctaa acaatttaaa cttaacatta cattagttat
    61801 gaatcaaaag aaacgcgttc atttgaacta aatttaaaaa acaatcgtct ccttgcagaa
    61861 tataccgcaa aattaacgat tgtttttttg ataaaatcta tgaatcactg ttctgttaaa
    61921 aagtctttga tccgtttgca ggcctcatgt aatttttcca tgctgctagc ataagaaatc
    61981 cggatataac cttctccttc tgggccaaat gcaattcctg ggatacacgc aacttttgcc
    62041 tgttccgcta attctacaca aaactgccaa gagtcagttt gaatggcagc tgggagtttg
    62101 caaaacaagt aaaatgcacc gtttggctgg gtcacttcaa atcctaaagg cgccaattgc
    62161 tggaccagat agtctcttcg tgtttgatat tgctctttca tattctcgct atctttcacc
    62221 ccatttatta gcgcttcagc cgcagctttt tgcgacatcg ttgtagcggc agtcaccaga
    62281 tattgatgga ctttaattag ttcgtcaata agggcttttt gagcaaaaat aaagcccact
    62341 cgccaaccag tcatcgcatg tgacttggat aacccgctta tcacgattgt ttgttctgga
    62401 agataagtag ctatggacat atgcgttcca ctataagtca attcagcata tacttcatca
    62461 cttataacaa ataccggata tttttttaaa acctcagcga ttgctttcaa ttgttttgca
    62521 gacaatgtcg tcccagtagg attgttagga tagtttaaaa tgactgcttt gacagctgat
    62581 ccatattgtt caaatgctcg ttcaaattgt tccggctgac agacaaaccc tgtgtcagaa
    62641 gtatctatcg ggatcaattc agcaccagct agttccacta atggctgata tcctgaataa
    62701 gcaggcgctg gaataagaac cttgtctcct tcttccaaaa cagataacaa cgcagaggca
    62761 atcgcttccg ttgcaccaac tgtcgtcaaa acttccgttt gcggatcata agttaatcca
    62821 taacgctgtt gctgaaatat acaagccgct tcccgcagtt cctctaatcc tctcattccg
    62881 gtatagtgag aaaagtcttc ttcaatcgct gctattcctg cttgcttgac atgttcagga
    62941 gttggaaaat ccggttctcc cagggtgagt ttgatcacat ctggtatcga cgatatttgc
    63001 tgatcgaatt gtcgtatttt tgatacttct attctagaaa gcctaggact aaatcgattg
    63061 gataaattca tcattattta ctcgctcctc ttttgaactg ctatcgattt ttacaagaag
    63121 attttctgta aaaagaatcg ttttgtacca ttctacacca ttttctaaaa catccatatc
    63181 aaaatcgaac gtcggatcat gtaaagcagg ggtatcaccg acacctaaaa agaaaaatac
    63241 acatggatat tgttgcgtat atatgccaaa atcttcggct tgaagaactg gagcatctaa
    63301 ttcatgtagt ttgtttgaat ctaacagtgc tgcatataag cctctatggt tgattacagc
    63361 tggatagccg tcattatagc gaaaagaaat agtagctgta gagtgaaact gttctgctaa
    63421 atggtctaat cccctcttca aataattttg catatctgaa ctgtaagaac ggatacttcc
    63481 ggatacaata acttctccag ctaaaacatt tcggatcgtt ccaccttgga tttggccaaa
    63541 tttcattaga tgattccact ctgccggtaa ttgccggtca aaatcataaa cattcttcaa
    63601 aaatcgtaca gctgcctcca gactatcgat tcctttttca ctagaagcaa tatgtgcaga
    63661 ctggccttta atgataatgt ctgtttcact gctttgtgcc atcaaaggtc ctggtcgaga
    63721 gaaaacttct cccttagcta gtccaggcca tagatgaaag ccaaatattg cttgtacttc
    63781 atatttatcc aacaatcctg actgaataac gctttgtgcg ccaccggaaa tttcttctga
    63841 tggctgatag atcagtacta cattataaat aagatcctca atgtgtttag ttataaagtc
    63901 agtcaaccct aaaagcatcg ccatatgtcc gtcatgacca catgcatgca tataaccttc
    63961 atgcttagaa gcaaaagaga gattcgtttc ttctttgact ggcagtgcat cgatatctgt
    64021 tcgaaaagca atcgtttctt cttgctgatt atcaaaataa aggagtagtc ctgtttcgcc
    64081 gatttcatgg ataacggcct cgctgttttt tacttgttca tataaatatt tttttgtctt
    64141 gaattcatga aacccgattt caggaatttg atggagtgcc attcgatatt ctttcagcat
    64201 ccgtattcac catcccttca tagataccct tcactactgt ttcatattga ttattcggta
    64261 caccgatgat cagattcaac tcctgtgctc cttgtgataa gatcgatgtg cgaatttcta
    64321 atttgttcaa tatcgataac actttcccag ataatccaat caattcttta tgcggacccc
    64381 caacgacaga gatcaaagct aagtcttcga tcacgacgat ttcttccacg ccaagtgttt
    64441 cttttaattc tttcgtgatg agaaataact tatcggcaat cgcttctgct gagacgatca
    64501 ctccgatatt gtcgatccct gaaggaatat gttcgattga tatctcatgt ttagcaaaaa
    64561 tgctcattgc tttccagata aacccgattt catcggacat atgacgtttg aataaagtga
    64621 tggaaagaaa atccttgcgt ccagctattc ctgtcagtcc attttccttt tctatttcgt
    64681 gattcgatat ttttgtccct tgagcgaaag gatcattggt attttttatt tggatgggga
    64741 tatcagcctc ttgaactggg aaaacagctg cttcatgaag aacgttagcc cccatataag
    64801 ccatctctct cagttctcta aaagagattt cctcgatgat tttaggatga tcgatgattc
    64861 gcggatcagc catcatgatt cctgaaacat ccgtccaatt ctcataactt tccgcttgca
    64921 acatcttcgc cagtatcgcg ccggtaatgt cgcttccccc acggcccaat aatttttctt
    64981 ttcctgcagg gttcgccccg taaaaccctg gaacaaccat tttcggatat cttttaaatg
    65041 cttttgtgat ttcctgtgct gttttttcct gatcgatttt cccatcatag tcaaagaaaa
    65101 tcaggtcttt tgcgtcaata aactggtaac caagatactc agccatcagt tgagcagtca
    65161 gatattcacc tctacttact aaataatctt cagttatctt ttcgtcttcc agttgtttct
    65221 gtatgcgtgc tagttcatct tcaataggta actgcagggc aagaacatct cttacttgaa
    65281 tgaaacgatc acaaatcctt ttccaaaggg gtgtccaatc aacatgatgc tgaaggtaag
    65341 cataaatcaa ataaagtaag tctgttactt tgtcatcatc tgacgtacgt ttgcctaaag
    65401 cagaaacaac gatcacttgg cgattgggat ctgaaagaac aattgtacgg acttttttga
    65461 attgctcttc atgagacatc gatgttccgc cgaatttcgc tactttcagc gtattactca
    65521 tcttgccacc tccgctattt tctgcattaa agctgcagca atctgtacgg cattcgcagc
    65581 ggctcctttg cgtatgttat cggcagtcgt atagaacaac aatccgttct ctaaagaagt
    65641 gtcttttctg atcctcccaa cataaaccat atctgttcct ctagcaagaa tgctcgtcgg
    65701 atatttatgt gctgctagat catcttctaa ttgaatccct ggaaactggc tgagacactc
    65761 tctaatttct tccgtcgtaa actctttttg cagttgaact gccacagaga ctccatgtcc
    65821 attttcgatt ggcacacgta cgcaggttgc tgatactggc aaagcaggct gatgcagaat
    65881 cttacgggtc tcatttatca ttttctgttc ttcttttgta tacccatttt ccaaaggctg
    65941 atcgatttcc ggaatcaccg tttggctaat attataagga tacaaaactg gttcctcgtc
    66001 attttgcgta cgatgaagat cttcaatgcc tttttgcccg gaaccagaaa ctgcttgata
    66061 ggtcgaataa ttgactcgct ggataccaaa ggcttcttgt aaagctttaa gcggaagtac
    66121 cgcttgaatg gttgaacaat ttggatttgc aatcagccga ttcaatgtat agtcttccag
    66181 attgacctct ggaacgatta atggaatatc cgaatcttcc cgccatgctg aggaattatc
    66241 tatgacaaat agtccctgac ttcgtgcgat tggcgcaaac tctttggata cagaagcacc
    66301 tgcagaaaat agcgctaaat catatccttc aaatgaagtt tctgtcaatt cctgtacagt
    66361 gtgtttcttt ttcttgaata cacaaacttt accggctgaa cgttttgaag ctaataattt
    66421 caatcgtcct actggaaaat catattcttc taacactttg agcatcgttt ggccgactaa
    66481 tccggttgcc cccacaatac aggtatctat ttcgcgcatt tttctccatc tcctctataa
    66541 ttcgcgtaac gcagcaatca atgctgtatt ttcttttgtc tgatcatcac ttatttttaa
    66601 tacacgtgca ggtattccac cgacaacagt ttctgctgcc acatcttcta aaaccactgc
    66661 tcctgctgcc acgactgcat cttttccgat atggacacct tcaacaatca ctgcattcgc
    66721 acctaccagt acgccgtctt ctacgataac cggtttggcc gatgctggtt cgatcacacc
    66781 tgctaagaca gctcctgcac caatatggca gtttttgccc actgtcgctc gtccacctaa
    66841 gacagcaccc atatcaatca tcgtgttttc accgattact gcaccaatat tgataatcgc
    66901 tcccatcatg atcacggcat tgttgccgat tgacacttga tctctgataa tagcacctgg
    66961 ttcgatacga gcattaattt ttttaatatc tagcaaagga actgcgctgt atcttgcagc
    67021 agtttcgatt tcgatttcaa tgattttttc tgattcagct tctaaaaccg gagcaatcgt
    67081 ctcatagtca ccaaaaacaa cgaagctgct tccttcacca aaaactttac tttctttaaa
    67141 ttcgattggc tcggttacat tcagatagac tttcacaggc gtctttttag atgccgtttg
    67201 aatgtagcgg atgatttctt gagcttccac gttttttaca cccctttgtc cattaataaa
    67261 tcttctaatt tgtaataacc tggtgactga tctttcaacc agtccgctgc ttctactgcg
    67321 cctgcagcaa agattcgatt agaaaaagat tcatgtttga tcgtaaatat ttcatcagta
    67381 gcagcaaaca atacatcatg ttcaccaggt aaagaacctg cacgaatact gtgtacacca
    67441 atttggtttt cttctctttt tttcccttgc cagttgtata cgggctgcag cttttgcact
    67501 tcttggattg tttgaagcag catatttgca gtaccagatg gtgcatcttt tttacgacta
    67561 tgatgttttt caatcagttc gatctgccag ccttgcaaca ttgcagctgc ttgcttgatt
    67621 agttgattca tcaccatgat gccgaacgaa aaattagcac tatacatcac aggtacatgt
    67681 ttggcatgtt cttttatttc ttggaactgt gattgtgtat aacctgtcgt gccaatgact
    67741 aacggaagtt gatatgataa gctatacgaa aggattttct ctaaattatc tggatgagaa
    67801 aaatcaataa gcagttctgg cagttccgta aaagcaagcg tagttgcttc tttaggtgcc
    67861 gtccgtaaaa gaaggaccgt atctcctctt tcttgtgcaa cttcttcgac tctatttccc
    67921 atctgtccac tgccaatcag tccgattttc atttttcaac cgtcctttct tttaacaaaa
    67981 gctgagctaa ttgtttcttt ttctcttttg aaagagaaac taaaggcagg cgtagaggtc
    68041 cggaaggtaa gtctaataat gacatggctg ctttgacagg aataggattg gtctcactga
    68101 acaaggcatg gatcagttca agtgcattta attgcttttc ttttgcttgc tgccatcttc
    68161 cgtcctgcgt atcttttact aattcatgga ctattttagg ttggatattc gcccatacac
    68221 ttattacacc gcttcctcct acactcatta ctggtaggat caaatcatca tttccactgt
    68281 aaaggaagaa ttcttctcca atcaaccgag cggcatccat cacataagcc atatctcctg
    68341 aggcttcttt caaaccaatg atattaggat gttctgctaa atttactaat acatggattg
    68401 gaatcgacat gcctgttcta gaaggaacat tatacaaaat aagcggaatc ggtgaggctt
    68461 ctgcaattgc tgtaaaatga gcagctagtc ctgcatcact tgttttgttg tagtaagggg
    68521 tgatgaccaa aagtgcgtct gcccccaaag aagcaaaatg cttcgctttt tcaatcgttt
    68581 ctttcgtatt attcgttcca gctcctgcaa tcaccggcac acggccagcc actttcttta
    68641 ctgtcagttg aaggatctgc tcctcctcag attgtgtgag cgtagaagat tctcctgttg
    68701 tacctaaaac taataatcca tctgtttgtt catctaaatg gaattggatc aatttttcca
    68761 aaccagcata atctactgat ccctcttcat tcatcggcgt gatcagcgcc acgatcgaac
    68821 cttttatcat cttcattcct ccaatattct tgtccattca tgaactgctg ggccagccat
    68881 ttgtatcgca tcctcaccag agatcatcaa gtctccttgt tccaagtgaa caactacctg
    68941 atctgctgaa attttcccag tttccttagc aaccacataa gaggcacagc aacctgtacc
    69001 gcaagcaagt gtccaaccta ctcctctttc ataggtacgg acgatcaatt cttgttcatt
    69061 gactacttct acaaagttga cattggtttt ttctttgaac aaaggatgat gacagatcag
    69121 ttcacctctt tttctccaga cttcctcacg cgcattttca acaaaaacaa cggtatgaat
    69181 cgttcctaaa aataaactag tgatcgttac tttttcacca ttgatctcta tttcctggtt
    69241 ttttattgga taagtttgat tagctttgat tttttccgga gaatcatctg gcactcccat
    69301 atctacgctg caataaaaag gtgagggatc caacaccgta atttttcttg tgcctgctaa
    69361 tgtctctacg ttgaattcct gttcattcat catgttctga tcatttacgt atcgagcaaa
    69421 acagcgaatg ccatttccgc acatcggcgc gcggctgcca tctttgttgt aatagatcat
    69481 ttccaacgga tcgattttta cggcgatcaa tccgtctata tcatattttt cttttgtgca
    69541 taaagaagcg gctaattgac tataatctgt gtcttctgcg taatctaata agataaaatc
    69601 atttccgcag ccattatatt tatgaaggat cataggaaac ttcccccctt agtagatcag
    69661 aaaatgcttg tctgcgaaca atcagtcttg attggccatt attgacaaaa acaactgctg
    69721 gtcgtgtcgc tcgattgtag ttgctgctca tcgagtatcc ataagctccc gtggaataaa
    69781 cagctaaaag atcacccgat tctgctttcg gcaaataagc ttctttaatt accacatcgc
    69841 ctgattcaca catttttcct gcaatcgtca ctttctctgt tttttcttcc gacagttttg
    69901 ctgctaaatc acaatcgtat tctgcctgat acagagctgg acggatatta tctgtcatcc
    69961 cgccatctac aaagtaatat tttttatgtg gtgtcttttt gatgaaacca atcgtgtata
    70021 gtgttgtacc agcttctcca acaatacttc tacccggttc gatcagtaat tgacgaatgg
    70081 tcagttttcg tttttccaaa gcttcttccg tatattcaat cagttttttt agtgcctctt
    70141 cgactgggat cggctgatca ttttgtgtat aacgaatccc aaaccctccg ccaagattca
    70201 aggaaagagg ttggtcaaaa tcggttaagt aattcgtcaa tttgtcgatt tcagctaacc
    70261 aagcagtaac atcaaaaatt tgtgacccga tatgagcatg aaatccttct aactctagat
    70321 agctgctttc ttttatcaac tgaagggctt gttggcactc tttactttca tagctcatgc
    70381 cgaattttga atcaatatgc gcagtgacga tatattcatg agtatgtgct tctactccga
    70441 cattcaaccg aagcatgata caaatttttt ttaaatagcg ttggcttaat tctgctaata
    70501 attcaacttc catcaaatta tcggcaacaa tatgcaacaa atcattttca agtgcataga
    70561 tcagttcctc tggcgtcttg ttgttaccat ggaagtagat agtatctcta ggaaaatcag
    70621 cttgcaaagc ggtatagatt tccccaccac tgacaacatc tagcccgaat ccatattcac
    70681 gaactaaatg cagcatctca atcgtttgga atgctttaga ggcgtaaagc acttttgtat
    70741 caaaacgttt cgatacaaag cctcgcttga atgacagcat cgtttgtctc atcttttctt
    70801 catcatacac atacagaggt gtcttatact tttctgccaa tgtgcttgct gtgactccgc
    70861 caattatcaa ttctgtcatt ttttcattcc tcttttcact ttttttccgt ttttattgga
    70921 atttcatttg ataaagattt gaattttatt ggaataatat taaacaaaat tttctgaatt
    70981 gtctagtata tttcgaaata tttttttaaa aataagaata aaaatggtta taaaaacaaa
    71041 atacccgaac tattagaagc tatttataca taaaaacata aattcaacac tatttagtga
    71101 tgaaaaaata ttttcgcttc aatatagttc ggaatagttc ggatattttc aattttatga
    71161 atcatttcaa tttcttcata aataagtgca gttttttctt tctctagctt ttgccattca
    71221 tctttcaaaa tataaataaa atcaaatact tttttatttt tcaatttgct gattccacaa
    71281 aaaacctctt gaaaggcaga tccgatgatc tgcctttcaa gaggtcaagc tctgttttca
    71341 aattcttatc aatccgttag cgaattattt tgacgcacgt tttacataag aaccttctgc
    71401 tgtattgatt tccaacaatt catctgtttc gatgaagtca ggaacattga ctactaagcc
    71461 agtttccatt gtcgctggct tacctgaacc agtgactgtt gcacctttga tggaaggttg
    71521 tgtttccaca acacgtaata cgacagttgt tggtaattga acgccgatga cttcgctacc
    71581 gaaaaattgg atttttactt ccatgttttc taggatatat ttcatttctt cttctactgt
    71641 ttctacagga atttcatatt gctcatatgt ttccaaatcc atgaagaaag ctgtatcatc
    71701 catcgtgtac aaatattgta caggttttgt gtcgatatgt gctttttcaa atttttcttc
    71761 tggtctgaac gttgtgtcgt atgttgcacc tgaacggaca tctttcagtt tcatacgcat
    71821 aactgtattt cctttaccag gtttatggtg gcttgcatcc agaactttaa tcagtttgcc
    71881 gtcttgtacg aaagtcatac cggctcttaa atcacttgct gagatcatta tttttgctcc
    71941 ttcttttctt tatcataatc aactttattg tatcagacaa ccggccagaa aactagagta
    72001 ttctctatct tttaaggtct catgcctatt tgtcagaaaa ataaacacgg gtaaacgatg
    72061 ctatcgaaat atttttttct ttcaagcctt tgacttttat tttttttcat gctatcgtta
    72121 ataaaaattt tacttggagg gatacacatg acagtcagca accaaaaaca attgaatcgt
    72181 tgctgttgta tggtgcataa aagcagcata ctatttgtca tgtctattaa ttgcccttca
    72241 tagaatgagt accaggtaac ttatcttttt atctactcaa tttttctcta tgtctttcaa
    72301 ggagctgatt taatagtcag ctcctttttt tattatcttt ttttaggagg cccacatgat
    72361 tatctcgaca attacagaag ctattggttc cacaccgctt tatcgctttg acaatcagca
    72421 ctattctatg cctaaaggtt ctgctattta tgcaaaacta gaatatttaa atcctggagg
    72481 aagtatcaaa gatagattgg gcagttattt gatcaaagaa ggatttgccc aaggaaaaat
    72541 caatgaaaaa acgacgatca tcgaaccaac tgccggaaat acaggaattg ggatcgctct
    72601 ggccgcactc acatacaaat taagaacggt ttttgttgtt ccagagaagt ttagcttaga
    72661 aaaacaacaa ttgatgaaag cattgggagc aaagatcatc cattctccta gtgagcaagg
    72721 aatcatcggt gccatcagca aaagtaaaaa acttgcagag gagatatcaa acagctatct
    72781 gcctcttcaa tttgaaaata aagataatcc tgctgcttac tatcatacgt tagggccaga
    72841 aatctttcat gaaatcaaag aaaatatcca tagttttgta gcaggaattg ggagtggtgg
    72901 aacctttgct ggaacttcta cctttttgaa agaaaaatat cccgacattc gaataatcgg
    72961 tgtagaacca gagggctctg tgttaaatgg tggtgatcca gcccctcacg aaattgaggg
    73021 tatcggtgta gaattcattc cgccgtttct ttctcccctt tcaatcaatc agatcgagac
    73081 aatctctgat gtagaagggt ttaactatac taggcagtta gcacgagaac aaggactatt
    73141 agttggaagt tcgagtggtg cggcttttgc agcagcatta agagaaattc gtcgtttacc
    73201 acctggtcat cgtgtcgtta cgatttttcc agacgcagca gaccgctatt tatcaaaaaa
    73261 catttactta taggaggaaa gaaacatgca cattcaaaca aaattgatcc acggaggcat
    73321 cagtgaagat cccacaacag gagctgtcag tgtacctatt tatcaaacat ccacttatcg
    73381 gcaagatggc gtcggccagc ctaaacaata tgaatattca agatctggta atcctactcg
    73441 ttttgcattg gaagaactga ttgctgattt ggaaggcggt gttcgaggat tcgctttttc
    73501 ttctggtctt tctgggattc atgccgtttt ttctcttttt caagctggcg atcatatctt
    73561 attaggagat gatgtctatg gaggaacatt ccgtctattt gacaaagttc ttactaaaaa
    73621 tggtctcgag tacacgatca tagatacgag taatcttgat aaaatagaac aatccatcaa
    73681 acccaataca aaagcacttt atcttgaaac acctagcaat ccgcttctaa agattactga
    73741 tttagaaaaa agtgccactc tggctcatca acatggattg atagttattg ctgacaatac
    73801 atttgctact ccttattttc aaagaccttt agatctcggt tctgatatcg ttgtccatag
    73861 cggtaccaaa tatctaggcg gacacagcga tgtagtagct ggtttagtga cttctaacca
    73921 taaagatcta gccgatcaga tcggatttta ccaaaatgct atcggagccg tacttggtcc
    73981 ccaagatagc tggttacttc agcgcggaat caagacacta agtgttcgta tggaggaaca
    74041 tcaaaaaaat gcttttgtgg tagctgattt tctattttct catccagcag tagaaaaggt
    74101 ctactatcca ggattacccg atcatgaact ccatggagtt gctaagcagc agatgagcgg
    74161 ctttagcgga atgatttctt ttactttaaa aaatgaagaa tctgctattc catttgttga
    74221 atcactccaa ctatttactt tagcagaaag tttaggtggc gtagaaagtt tagtggaaat
    74281 tccttctgtc atgactcatg cgtctattcc aaaagaaaaa cgagaagagg ctggcataaa
    74341 ggacggcttg atccgtcttt ctgtaggaat tgagtatggt caggatctca taaacgattt
    74401 agctcaggca tttgacagaa tcaagaacta gaagtcccaa taaacgactt agcagaaaaa
    74461 tctaaaaaat atttcagatt gctacacctt ggaaacgata agtgttagaa tcaagtagaa
    74521 agacgttttt gtaaatttca ctaaataatt ctttagtgaa aagcatcaaa acgaatgaac
    74581 aaacagatag gagtcttaca aatgagaatg aatgtcgaaa gttttaatct tgaccataca
    74641 aaagtcaaag caccttatgt acgtgtagct gatcgaaaaa cgggtgcgca tggcgatgtc
    74701 atcattaaat acgacgtccg ctttaaacaa ccaaataaag aacacatgga catgccaagt
    74761 ctacattcac ttgagcactt gactgccgaa ctaatccgca atcacgcaga ttacatcgta
    74821 gactggtctc caatgggatg ccaaacaggc ttttacttga ctgtattgaa tcatgaaagc
    74881 tacgaagaca ttttagatgt tcttgaagca acaatgaaag atgttcttga agctactgaa
    74941 gtacctgcaa gcaatgaaac acaatgtggc tgggctgcca gccatacact agaaggtgca
    75001 caagctttgg ctcgtgaatt tttagcaaaa cgcgatgaat ggtctgaagt agaagcatag
    75061 tttctaaaag aacgtaaaga tgagaagcaa tatagctatc ttatcttcgt attttattaa
    75121 atatttattg tctgaaaaga gttctaaacg ggaaaacaaa tagctagttt tcagtttagc
    75181 gctcttttta ttatataaaa tagaaaggag aaaactttat gtctatttat aattttgaaa
    75241 ctactttaga agacggcact gtctattcat tgaaaaaata tgcaggcaaa ccgataatca
    75301 ttgttaacac tgcaacaaaa tgcggtctag cacctcaatt taaacaatta gaagaacttt
    75361 accaagctta tcagacacaa ggtctggtta tcttaggctt tccttccaat caatttaaac
    75421 aagagctaga ttcagcgact gaagcagcag aagcttgtaa aaccacttat ggcgttacct
    75481 tccctatgca tcaattgatt gacgtaaatg gagaaaatac agatcctctt tttacttatc
    75541 ttaaaaatga agctcctggt actgtcacta aagcaataaa atggaatttt actaaatttt
    75601 tgattgatag aaatggcaaa gtaatcgaac gatttgcacc acaaacttct ccattgaaaa
    75661 tgaccgctgc aattgaatcc gttctttaac ttaataaaaa taaaaaaaga tgaactattt
    75721 ctagttcatc tttttgcctt tgcgtggcga cgtcctactc tcacaagggg caacccctca
    75781 ctacaatcgg cgctaagaag cttaacttct gtgttcggca tggttacagg tgtatccttc
    75841 tcgctatcgc caccacactg tggtgttatc ttttattgag taaattttgt tcactcaaaa
    75901 ctggatttga agtca
//
LOCUS       DASHFF010000007        63865 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.007, whole genome shotgun sequence.
ACCESSION   DASHFF010000007 DASHFF010000000
VERSION     DASHFF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.007"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(538..1995)
                     /gene="ascB"
                     /locus_tag="U2I75_000536"
     CDS             complement(538..1995)
                     /gene="ascB"
                     /locus_tag="U2I75_000536"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1695696.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1992..3887)
                     /locus_tag="U2I75_000537"
     CDS             complement(1992..3887)
                     /locus_tag="U2I75_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1695697.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3945..5720)
                     /locus_tag="U2I75_000538"
     CDS             complement(3945..5720)
                     /locus_tag="U2I75_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1695698.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(5770..7266)
                     /locus_tag="U2I75_000539"
     CDS             complement(5770..7266)
                     /locus_tag="U2I75_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1695699.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(7460..9082)
                     /locus_tag="U2I75_000540"
     CDS             complement(7460..9082)
                     /locus_tag="U2I75_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1695700.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(9410..10723)
                     /locus_tag="U2I75_000541"
     CDS             complement(9410..10723)
                     /locus_tag="U2I75_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1695701.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(10968..11192)
                     /locus_tag="U2I75_000542"
     CDS             complement(10968..11192)
                     /locus_tag="U2I75_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695702.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(11212..11634)
                     /locus_tag="U2I75_000543"
     CDS             complement(11212..11634)
                     /locus_tag="U2I75_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN1695703.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(11776..12486)
                     /locus_tag="U2I75_000544"
     CDS             complement(11776..12486)
                     /locus_tag="U2I75_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695704.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(12504..13001)
                     /locus_tag="U2I75_000545"
     CDS             complement(12504..13001)
                     /locus_tag="U2I75_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1695705.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(13038..13490)
                     /locus_tag="U2I75_000546"
     CDS             complement(13038..13490)
                     /locus_tag="U2I75_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1695706.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(13625..14230)
                     /locus_tag="U2I75_000547"
     CDS             complement(13625..14230)
                     /locus_tag="U2I75_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1695707.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(14680..15300)
                     /locus_tag="U2I75_000548"
     CDS             complement(14680..15300)
                     /locus_tag="U2I75_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1695708.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(15302..16171)
                     /locus_tag="U2I75_000549"
     CDS             complement(15302..16171)
                     /locus_tag="U2I75_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN1695709.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(16194..16601)
                     /locus_tag="U2I75_000550"
     CDS             complement(16194..16601)
                     /locus_tag="U2I75_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1695710.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(16620..17909)
                     /locus_tag="U2I75_000551"
     CDS             complement(16620..17909)
                     /locus_tag="U2I75_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1695711.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(17936..18238)
                     /locus_tag="U2I75_000552"
     CDS             complement(17936..18238)
                     /locus_tag="U2I75_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1695712.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(18263..20356)
                     /locus_tag="U2I75_000553"
     CDS             complement(18263..20356)
                     /locus_tag="U2I75_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1695713.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(20845..21609)
                     /locus_tag="U2I75_000554"
     CDS             complement(20845..21609)
                     /locus_tag="U2I75_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN1695714.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(21720..22589)
                     /gene="mdcB"
                     /locus_tag="U2I75_000555"
     CDS             complement(21720..22589)
                     /gene="mdcB"
                     /locus_tag="U2I75_000555"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN1695715.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(22614..23690)
                     /locus_tag="U2I75_000556"
     CDS             complement(22614..23690)
                     /locus_tag="U2I75_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN1695716.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(23714..24307)
                     /locus_tag="U2I75_000557"
     CDS             complement(23714..24307)
                     /locus_tag="U2I75_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN1695717.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(24311..25987)
                     /gene="mdcD"
                     /locus_tag="U2I75_000558"
     CDS             complement(24311..25987)
                     /gene="mdcD"
                     /locus_tag="U2I75_000558"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN1695718.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(25977..26276)
                     /locus_tag="U2I75_000559"
     CDS             complement(25977..26276)
                     /locus_tag="U2I75_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN1695719.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(26378..28024)
                     /gene="mdcA"
                     /locus_tag="U2I75_000560"
     CDS             complement(26378..28024)
                     /gene="mdcA"
                     /locus_tag="U2I75_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEN1695720.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(28031..28942)
                     /locus_tag="U2I75_000561"
     CDS             complement(28031..28942)
                     /locus_tag="U2I75_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1695721.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            29062..29919
                     /locus_tag="U2I75_000562"
     CDS             29062..29919
                     /locus_tag="U2I75_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1695722.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(30106..30468)
                     /locus_tag="U2I75_000563"
     CDS             complement(30106..30468)
                     /locus_tag="U2I75_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN1695723.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            30814..32253
                     /locus_tag="U2I75_000564"
     CDS             30814..32253
                     /locus_tag="U2I75_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1695724.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(32260..34811)
                     /locus_tag="U2I75_000565"
                     /pseudo
     CDS             complement(32260..34811)
                     /locus_tag="U2I75_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(34925..36256)
                     /locus_tag="U2I75_000566"
     CDS             complement(34925..36256)
                     /locus_tag="U2I75_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1695725.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(36222..37700)
                     /locus_tag="U2I75_000567"
     CDS             complement(36222..37700)
                     /locus_tag="U2I75_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1695726.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGSVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(38005..39840)
                     /gene="tdc"
                     /locus_tag="U2I75_000568"
     CDS             complement(38005..39840)
                     /gene="tdc"
                     /locus_tag="U2I75_000568"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1695727.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            40310..41737
                     /locus_tag="U2I75_000569"
     CDS             40310..41737
                     /locus_tag="U2I75_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1695728.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(41829..42194)
                     /locus_tag="U2I75_000570"
     CDS             complement(41829..42194)
                     /locus_tag="U2I75_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1695729.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(42191..42556)
                     /locus_tag="U2I75_000571"
     CDS             complement(42191..42556)
                     /locus_tag="U2I75_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1695730.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(42748..42996)
                     /locus_tag="U2I75_000572"
     CDS             complement(42748..42996)
                     /locus_tag="U2I75_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695731.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            42995..43123
                     /locus_tag="U2I75_000573"
     CDS             42995..43123
                     /locus_tag="U2I75_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN1695732.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            43180..44874
                     /locus_tag="U2I75_000574"
     CDS             43180..44874
                     /locus_tag="U2I75_000574"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN1695733.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            44878..44997
                     /locus_tag="U2I75_000575"
                     /pseudo
     CDS             44878..44997
                     /locus_tag="U2I75_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            45648..46781
                     /locus_tag="U2I75_000576"
     CDS             45648..46781
                     /locus_tag="U2I75_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN1695734.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            46812..47132
                     /locus_tag="U2I75_000577"
     CDS             46812..47132
                     /locus_tag="U2I75_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN1695735.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(47161..47625)
                     /locus_tag="U2I75_000578"
     CDS             complement(47161..47625)
                     /locus_tag="U2I75_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1695736.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(47756..49009)
                     /locus_tag="U2I75_000579"
     CDS             complement(47756..49009)
                     /locus_tag="U2I75_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1695737.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLASGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(49038..49355)
                     /locus_tag="U2I75_000580"
     CDS             complement(49038..49355)
                     /locus_tag="U2I75_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1695738.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(49381..49701)
                     /locus_tag="U2I75_000581"
     CDS             complement(49381..49701)
                     /locus_tag="U2I75_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1695739.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(50165..51580)
                     /locus_tag="U2I75_000582"
     CDS             complement(50165..51580)
                     /locus_tag="U2I75_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1695740.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(51597..52913)
                     /locus_tag="U2I75_000583"
     CDS             complement(51597..52913)
                     /locus_tag="U2I75_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1695741.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(53063..53761)
                     /locus_tag="U2I75_000584"
     CDS             complement(53063..53761)
                     /locus_tag="U2I75_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN1695742.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(54050..55010)
                     /locus_tag="U2I75_000585"
                     /pseudo
     CDS             complement(54050..55010)
                     /locus_tag="U2I75_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(55124..55957)
                     /gene="msrA"
                     /locus_tag="U2I75_000586"
     CDS             complement(55124..55957)
                     /gene="msrA"
                     /locus_tag="U2I75_000586"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1695743.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(56191..56934)
                     /gene="truA"
                     /locus_tag="U2I75_000587"
     CDS             complement(56191..56934)
                     /gene="truA"
                     /locus_tag="U2I75_000587"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1695744.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(57125..57760)
                     /locus_tag="U2I75_000588"
     CDS             complement(57125..57760)
                     /locus_tag="U2I75_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN1695745.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(57907..59148)
                     /gene="sstT"
                     /locus_tag="U2I75_000589"
     CDS             complement(57907..59148)
                     /gene="sstT"
                     /locus_tag="U2I75_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN1695746.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            complement(59469..60146)
                     /locus_tag="U2I75_000590"
     CDS             complement(59469..60146)
                     /locus_tag="U2I75_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN1695747.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(60397..60939)
                     /locus_tag="U2I75_000591"
     CDS             complement(60397..60939)
                     /locus_tag="U2I75_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN1695748.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(60944..61171)
                     /locus_tag="U2I75_000592"
     CDS             complement(60944..61171)
                     /locus_tag="U2I75_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1695749.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(61250..63130)
                     /locus_tag="U2I75_000593"
     CDS             complement(61250..63130)
                     /locus_tag="U2I75_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1695750.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            63416..63751
                     /locus_tag="U2I75_000594"
     CDS             63416..63751
                     /locus_tag="U2I75_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1695751.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
BASE COUNT        19095 a        13361 c        10730 g        20679 t
ORIGIN      
        1 ttgtcaaaaa acacggaata atgtccgcct cctctgtcta gatttgaaaa aaagaagtag
       61 ccatcttcaa agaatttcgg cttgttttcg acaaaactcc ttctggaaca ccgtgaatca
      121 gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa aattttgtga aaatggcttt
      181 ccacattttt gcataaattt ggcttaatac cgaaaaactg gcttttgaac accgtttata
      241 tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa caaaaatagt
      301 tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc agctgattgt acaccgttta
      361 ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt caaaaaacag
      421 cttctcatgt tttatgacta tggtgacttg tgatcgagga gttggcagga gaatactgtt
      481 tattcgtaaa gaaggggctg tgacaagact tttgtcacag tcccttattt ccttttatta
      541 taggatttcg ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt ttttctttaa
      601 ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca tagatatatc cataacgttt
      661 tgacatttgt gaagtagaag cgctgacgat atcaatgcag ccccacgacg tataaccaaa
      721 taaatctact ccatctttga tagcttcctt catctgctca atatgctgct tcaaatagtc
      781 aatgcggtaa tcgtcttgaa cggttccatc ttcttctact ttgtctatag ctccgagtcc
      841 gttttcagct atgaacagtg gtttttgata cctatcatat aattgattca aagcaattct
      901 caaaccctta ggatcaatct gccagcccca ttcacttgat ggaagatacg gatttttaat
      961 tcctccagtt attaagtttc cttggacccc ttcccaattt tctggatgag cactgactgc
     1021 actagacatg tagtagctaa aagaaacaaa atcaacaggg taggctgcta agatttcttc
     1081 atcacctact tctattttta agttgacacc tttttcttca aacattcgtt gcgtatacgc
     1141 tggataatat cctcttgctt gaacatcact aaagaacaaa gaagttcttt gctgctcaat
     1201 tgttttttga atatcatccg gatcacaaga atacggaaca agaagctgat agctgatcat
     1261 acaaccgact tgtgaacctt tgataatttc atgacaagct tttgtagcta aagcacttgc
     1321 tataaactga tggtgtgcac cttggtattt cgcttgttcc aaatggggat ggttttcttc
     1381 aattactcct acgctaacaa aagggtgatg ttttacacag ttgatctcat tgaaagtcat
     1441 ccaatatttt actttatttt tatagcgttt gaatactgtt tcagcgaaac gaacgtaaaa
     1501 ctgaataagc tttcgattag gccatccacc atattctgta accaaatata acggcatttc
     1561 ataatgggaa agagaaacga ctggctcaat cccatatttt agacattcgt caaacacttg
     1621 atcataaaaa aataatcccg cttcattggg tgtttcttca tcaccatgag gaaaaattct
     1681 tgtccatgcg atagacatcc gatagcattt aaaacccatt tcagcaaata aacggatgtc
     1741 ttctttaaag tgatgataga aatcatttcc gtgtctttta ggataataat acttgctgtt
     1801 gggatcctgc gccgctttta cttgctgatg cgtcatcata tgccattgat ctgtccattt
     1861 ttctttaggt aaatcgctgt cgaatgtata cacatcagac acagataatc ctttttcatc
     1921 tacatcccat gcaccttcac attgattggc cgctgtcgct ccgccccata aaaatccttc
     1981 tggaaatttt ttcatatcga tccccctaaa cttgctagtt ttcttgtttg attatttttc
     2041 ttatgactag taatgatatt tttgaatcta ctactatttg taacaataac cattgtggtc
     2101 ggatcatatc cctctttgat taattcttgc aaatctactt ccatcaataa atcgccttta
     2161 cgtacttttt ttccatcaga tactgcactt tgaaagtatt tgccatttaa attcactgta
     2221 tcaataccga tatggatcag tatctctgct ccttcttcac tgactacccc aattgcatgt
     2281 ttcgttggaa aaactgttga aacttccccg tttacagaac tgtagatttt tccgtccgtt
     2341 ggaatgatcg caaacccttc tcctaaagat ccactagcaa atgttggatc gtttacttca
     2401 gataaatcaa ttatttcacc tgctgcagga gtacttaaca ctatatcctt tgaggattct
     2461 tcttcgattt catccttgta gccgaaaaga aaagttagca tacaggaact gatcatcgct
     2521 aagaaataac tagcaactag ccaaacaaat ccttcaccaa tataaatcgg gaaagacaaa
     2581 atacttcttg tagcaactgc caaagcaccg gctcctgcgg caccagctac tccaccaaat
     2641 atagctccac atatacaagc aatgtaaaat ggtcgtttat atttaagagt cacaccataa
     2701 acagcaggtt cagtgattcc aaataatgct gtgataaatg cagataaaga taaacttttt
     2761 actttcttat ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg cgcagctacg
     2821 gcagtcatac atgctggacc tagcgtatca cgaccgtaga aagaaagatt gttggtcatt
     2881 actggtacaa gtccccaatg taacccaaaa atcactaaca cttgccaaaa tccgccgata
     2941 acagcaccag ataaaacagg gctaaagtta taaacagcta aatatccagc tgcaatcagc
     3001 tgacttgcat aatctgcaat tggacctaca actaataaaa taattgggac catcacgatg
     3061 atacaaatga atggtgtcaa taaatttcga atactttcgt gcaagctttt gcgaatctta
     3121 ggctcaatat aagaaagaac ccatacagct aaaattgctg gaataaccgt acttgtatac
     3181 cttgctagaa tcacgggcat ccctagaaat cgcaaggtta tgctatcact atatgcactg
     3241 acaatcgtag gataaacgag agctgctgca ataaccatag cgatgaaacg atctgtattg
     3301 aacttttttg cagcagtata tgccaaaaat ataggcaaga aatagaaaaa tgcatcagct
     3361 gctgcattca aaatgatata agttcctgat tcagtagtta ataaattcaa actcgtacat
     3421 aaggcgagta ctcctttgat caaaccactt cctgccaaga cgcctaaagt cggggcaaaa
     3481 acagcggcga tcatgtctat aaaagagttt aaaatgcctt ttttctcagt tcctctcttt
     3541 ccactagatg catcatcttc tagtttcatt tgtttggata atacgtcata cacttcattt
     3601 actgcatttc ctactacgac ttgaaacatt cctccagctt cgactacagt gatcacacct
     3661 tctagttgct ctaccttctc cttatctgcc ttcttattat cttttagggt aaagcgtaac
     3721 cttgttgcac aatggtaaac attgctgaca ttttcttcgc ctccgacaaa tcggacaata
     3781 tctttcgcta attgtgaata gtccattttt acttccccct tgttaattaa tgccgtagca
     3841 atccttctta caagataagt atagttctta gaaaacggtt tctccattca attagtaccc
     3901 cttctaatag cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc gagcaaatca
     3961 ctcactgctt gaaagaaaac aagtatatcg tttctcttat agtcaaagga gacaaaaaaa
     4021 acacgcttga tcttcctttg ctgatgtata agtggtatct caagctgata ctctgtgatt
     4081 tttgtttcta cttggctttt gataagacag cacaacaaag tctcggcatt taacggaagc
     4141 ataactaggt cagaactagg agagattagt tgctgaatgg aatctatctt tttatggatt
     4201 tttatttgat tttcttcttt gtagtaatga tcaagcagtt ttgttttctt tttttcttca
     4261 actatggtca gcttttcacg caaaatctga taaatctccc aagattcaaa ctcatctaga
     4321 acactaggta gataaatcaa atcatcccac tcaaaaataa gctcttgctc agtagtcaaa
     4381 attgcatcat attccgttat tttgtttgat tgatttttga aaagatacac tggttctatc
     4441 cggcattctt tgatccacat gttcatactt tgttgaatct gtttttttag tgtattcaaa
     4501 atactaattg gctggttact aacaatcagt atctcccctt gttttcgaaa aggattcaac
     4561 gcttcggcaa gatacaaaac aaaataagcg ctttcagcta aattgggacg taattgaaaa
     4621 tgtattggga agaaacgccg aattaaataa atttctaaag gataacggga gatgatcttt
     4681 tgtacaaaat gattcatggt attatgtccg gcatccatcc tcgttttcat ttgtttgata
     4741 tgggttagca aaaattctgt ttcatggaac aataacgaaa caggaagatc taaaaaacga
     4801 ataatcgttt gctcaaatgc tcttagattc ttctctattt ctttgttttt ttcatcatta
     4861 atcatcaaat aatttaactc taatagtcga ttttcaattg cttgtttttc ttgattggaa
     4921 aaagaatagg gtagttctga tgataacttt tttgtgaggg tctctaccat cggaataaat
     4981 aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt caaaacgaga
     5041 taccgagaaa atcgtatgaa gtcctctcct gaaacaatat aattatatga tataagaacc
     5101 tcttttaatg tcttttggac agtcggcatg atattggaaa aaattgtact gatttgttga
     5161 ggaaaccttg cgtgttggat caccgtttct gttccaacaa gagataagaa aattcttttt
     5221 gaattttctt cagcaatgat ttttagtcct tttgtcgacg aaacttccag ttctaattta
     5281 ggattgcgct cgatccaacg caaaatcgtt tttatttgta atgatacggc tgtttttgaa
     5341 agaaagaaaa tgtctgctat cttttgcata ctgacatacc ctttttgaaa caataagtac
     5401 gtcacaatag ttgaagctcg gaaatccatg ctggaataga ttttatgttt cgtcacctct
     5461 tgtatgattt gctgtttagt ttcagattca attgattcta aaaaataacc tgaacgttta
     5521 tttgactgga taatgatttt cttctcgtag atagcaagca tatttttgat ttcagtttgg
     5581 atcgttttct tgttggtacc taaagcttca gctaattctt cgcttgttat ccaatgattc
     5641 ttatttctta atactacaag aatttggagt tgtcgtttat ttaagtccat ttgcttacac
     5701 ctcctctttg ttataaacat acaggaaaaa cctctcaaat gacaatagtc acttgcagag
     5761 gtttaaaaat catttgaaga aagatctata ttcaaggttt ttaccattag actggataat
     5821 ttgtttataa tattcaaaac tatctttttt caatctccta aagcttccgc tcccatcatt
     5881 ttgtctatcc acataaataa agccatatcg cttcctcatt tcccctgttc ctgccgatac
     5941 gatatcgatt ggtccccacc ataagtaacc aatgatttgg caaccatctt ttattgcctc
     6001 agcaatttcc tgaagatgta tttttagata ctcacaacgt tttggatcgt ggattcgatt
     6061 caattcatct ggtgcttcat ctagtccaat gccattttca actatgaata atggtaattc
     6121 ataacggtct gtcaattcat tcatgacata acgaaaccct agaggatcaa ttggccaact
     6181 ccaaggttcg ggagattttt ccttcaaaaa gggattatcg aaaccatttg ctccgccagt
     6241 atctacgcgc atttctgctt cttttttata gacagcgcta cgataataac taaatgcaat
     6301 ataatctgcc gtgttagctt gcaagcactg ttcgtctcca tctaacatta ctggtgcaca
     6361 gtcttcttcc ttccatgtcc tgtaaatata agctggataa tgccctcggc acatgacatc
     6421 ggagaaaaac catgaattgc gtttaaattg cagtgaacca aatacattct ctggatcaca
     6481 gtcatatgca taagtcgcta atgcagaaaa gctacacata atacccatct tagcatttgg
     6541 atcgattttc tttaataatt gagtagcacg ggcgttcgct agaaacatat gatgacaggc
     6601 ttgataaatc actttttttg ttagtgtagc aatgggttca agtttatctc tagcagggaa
     6661 tgtaaccaat ccagcagctg cataaggcat acgaaaagca ttattgatct cattaaaggt
     6721 catccaatat ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa
     6781 aaagtcgatt aattttcgat tttgccaccc cccatactct cctaccaatg ctaagggggt
     6841 ttcataatga ctaagagtaa ttactggctc gatatcatat cgatttaatg tattgattag
     6901 tctgtcataa aatacgagac cagcctcatt tggcttctgt tcgtctcctc ttggaaatat
     6961 tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct aactccttca gtaaacgaat
     7021 atcctcttca aatcgatgat aaaaatcaat cgcttcatga gaaagataat ggagtgattc
     7081 gtctaaagca atctcccaaa tctttttttc ttcattccac tgaatactag gatgctggtt
     7141 catgatcccg ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca accatgcacc
     7201 ttcacattga ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa atatttcctg
     7261 atccataact atctctcctc gctatcaaca atttcttact tgaagtatag ctcgattcat
     7321 caaaacaaga tattctaact atttcggcag aagagaagaa aaaaattgaa tgctactctt
     7381 catttttaat atttatatat aacaaataca taagacgatc cccgaaagaa aacactcgtt
     7441 aaccatctaa gcaaacgatt tattttactt ccaacacaaa tgcttcataa ggtcgtaaat
     7501 caactttgcc gtctggtaac tgtttcagag gataatttga cacaataacc cttaaagctt
     7561 cacgtggaat agtgaacgtc tgcttttcac tggacatatt acaaataacg agccattgct
     7621 ggtcttcata tatgcgagaa taggcaaaga catgctcagg cgtcttttcc actaattcat
     7681 agtctcccca gacgatgatg tcatgttttc tacgcaaatc gatcaatttc ttgtacatat
     7741 aaaaaacaga atcttgatct gctaacgctt cttctgcatt gatttcttta taatttggat
     7801 tgactcgaag ccatggaatt ccagtagtaa acccagcatg ttcacgggcg ttccattgca
     7861 taggcgtcct agcattgtct cttcctttcg ccttgattga ttccaagatt tcctcttttg
     7921 taaatccaga agaaatcctt tcgttataca tattgattgt ctcaatatct tttgcttccg
     7981 cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc tccttgatag atataagggg
     8041 tgcctttcat catatggagc aagatagcaa acatcttggc actttctttt cgatacacat
     8101 cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg attattccaa aataaactat
     8161 tccatccttc atcacctaaa gatgtttgcc atttggataa tacttttttc agttcagaga
     8221 tactcaaggg tttcaaatcc catttgtctt tgccttcttg ctgatctaat cctatgtgtt
     8281 cgaattggaa gaccatggat aattcatttc ttttaggatt ggaataaagt ttagcgattt
     8341 cggtagttgc tccccatgtt tcgccaacag tcatcagatt tttgtcacca aaagttgctt
     8401 gattcatttc ctgaagatac tcatgcagtt taggtccatt acttgtgatt tctttatccg
     8461 gttctttgcc aatcatatcg atcacgtcca tacgaaagcc cccaatacct ttatcgatcc
     8521 agaaattcat catttgataa atctcttggc gaacctgttt atttttccaa ttcaaatcag
     8581 gttgacgctt gctatacaag tgcagaaaat attgccccgt tttttcatcc aactgccaag
     8641 cagaaccact gaatattgag cgcaatctat taggctcttt tccattgacc ggatcacgcc
     8701 aaatataata gtcccgataa ggattttctt tatttttacg ggcctcaatg aaccacggat
     8761 gttcatcaga agtatgattc accaccagat ccatgatgat acggatattt cttttctttg
     8821 cttcttcaat cagttcttcc atttcttcca tcgtaccgaa ttctgccatg attgcttgat
     8881 aattactgat atcataccca ttgtcatcat tcggagactc gtatactgga gaaagccaaa
     8941 ttgcatcaat acctaaatac gccagataat ccaacctgga aataatcccc cgcaaatctc
     9001 caataccatc ttgatttgaa tcttgaaaac ttcttggata gatttgataa acaactgaat
     9061 ggtgccacca tttttgttcc atgattttcc tcctataaat ggatgtttct attttttgct
     9121 taatcattta ttttagtctc tctcactaaa aattgtaacg ctttcaaata gctattgcac
     9181 cttgttatcg gtaacagaat aaaacaaaaa aacagctgag gaaattccct caactgtttt
     9241 ttgaaaggat tgatttatgc agggaagaat gctgatttca caacttcttt ataaacggtg
     9301 ttcactcagc tgtcccttgg tattagctca aaaaacggca aaatataaga aactattttt
     9361 tcgttttttt ctctaatact caggccagaa cgctgatttc acaacctctt tatttcacat
     9421 tcaatatcca catgaatccc acaaatacaa agaacagacc atacatgatt ggagatactt
     9481 cttttcctct tttagctgct aacatggtaa ttggatagaa gataaaacct aatgcaatcc
     9541 catctgaaat actataagtc aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa
     9601 tctctaagtt tccccattcg atttgtcgca aagattgtgc catcaatact cctaccataa
     9661 tcaatgctgg tgcagttact tgtgaagtaa caactgatag taatggagaa aagaacaagc
     9721 ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga
     9781 taccagctga tgattctaca aatgcaccaa caggagaagt accaaataag gaacccgcaa
     9841 gcatagctgt tgaatcagaa gctaaagctt tccctacacg aggcatcttg ttgtctttca
     9901 tgaaacctgc ttggtttgct aatccaacta aagttcctgc tgtatcaaag aaagtcacaa
     9961 gcaagaatgt cagcactacg acccacattt gaagagaatt gatgtcccct acatgtttta
    10021 atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc aacaatttca gaaggcattg
    10081 gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc catcccgatg aagatgccac
    10141 ctggtacacg ccgaacaagc aagatagcag ttacgactaa tccaaaaatc gtcaaccaag
    10201 tagaaccaac gttgagtgat cctaatccta ctaatgtaga ttcattcgaa acgatgatcc
    10261 caccttcgct cagaccaagg aaagcgataa atagaccgat accgccagaa atcgcatatt
    10321 tcaaatctgc tgggatcgca tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa
    10381 taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt ttcccatgga atccccattc
    10441 ctaaacaaac agaataagca aaaaaagcat tgatcccaag agcaggtgcc gttccaattg
    10501 gataacgggc taataccccc attaaaacac agccgatcgc actggctaat gcagtcgcag
    10561 taaacactgc tccttcatcc attccagaag cacctaatac agttggattt acaaataaga
    10621 tataagccat tgagataaat gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt
    10681 tcaatttttc tatttcgaag taggaaagta ttttttcctt catcttccat ccccctaaat
    10741 ccgaacatta taaataaagt ccgttaaaat ttcgatttat taataaaaca acgtgtttta
    10801 tcatatacta aaacaaaaca taagtaaaca aaaaattcgg attttaagca aaatccgaac
    10861 gttccatctg attaaatccc tttatctcaa agttcatgaa cattcacttt ggattacaag
    10921 aatttcttta ttgtgttaat aaagtttctt cattctagtc tcaaaaatca atagtgtgat
    10981 ttgattttac catttcgttt ctgttcttct ttgatgaatt cttttgtatc gacaggattc
    11041 agattattta gtccgccaaa acctgtatcg accatttttc cccagccttt tgtcttcttt
    11101 tttcctgatt tgagattctt tgctcgaagc ttctcactat gttttgctcg ccgatacttc
    11161 aaataacctt ctaatcttcc cattagatcc actgtttttc ctccttttta ttcaatcaga
    11221 cgactcagct tttcctataa caaaataatt gccttcaggg tcagaaaaat tgaaagtcaa
    11281 gatgccattg ttatctgtga tttctcctgg ttcttccatg ttctcgtaga tagtttcaaa
    11341 ttcatccgta aaaaatacta aagaaggcgg tggtcctaat acctctggtg aaaacttctg
    11401 cacaaatact tttgggaata cgcctaattc taattctttt gagatgctga gtacaatact
    11461 cttaaattct tctggcaatt cgattgtttc agaaatttct gcaccaattt tttctacaaa
    11521 gaatcggcaa atcatctcta cgtcattgac ataaagcatc acacgtgttt tttgtatcat
    11581 catttgaccc ttctttctat ttgattttct ttcctgtcta taaactgatt ccattatatc
    11641 acgaaagcgc aaacaccttg tgtttgatac tattcaagaa aaataaagca atagtttcta
    11701 ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa cggcaatgaa tgccgctctt
    11761 cttatttcga ttcttttata attctgcttc tgattcatct ttggttagtg tgtgtctaac
    11821 actagttacg ccaaatgttt gatcgatttt ttccttcaac gcttcgattg cctgtgcgcc
    11881 acgtgccgct tgttcagcat tgatggattc aatgaccagc accgctgctt gtgtttcttc
    11941 cgtcagcatc gttctttggg cttcacgcca gttgagacag cgacagatcg ctccttgttc
    12001 atcatagtag atgatttctt ccggaagtgc cggagcatcg gaatctgcac ctaaaggcag
    12061 gaatgattcg ccaccttggg cttttcctaa atgcagattt cctgcgatct tttcgatatc
    12121 ttctccgccg caaggtacgc caaaagacaa ggaaatacta ttatagatat ctacaagcgg
    12181 attgattggg aaaaattccc gttcttgatt tgcacgtttc agtaaagctt caatagaaga
    12241 acgtgcacct tttttagtct tgaatgtggt aaatgcctga cgccactgag caatgacttc
    12301 gttttggctg aatgtttctt ctgtaaggta atttcttgct tcttttgctg cctcattcaa
    12361 taattgatga tacgtttctt cactttcttt tgttacgtgg ttatcgatac catttacgga
    12421 taagatttcg atggttgctt caggaaatac ttcccagaaa ttggtatcca ctattacttt
    12481 tttcatctgt tcacacctct tgattagaat aattgcttga tgaatagata gtctgtttgg
    12541 ggatcatctc ccatataaaa aacatgttcg ctgtatttga taaatccttt tttacgataa
    12601 aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc aaatggctgt tttttgcttt
    12661 tcttttgctt tttcaatagc tgtatctagt aaaaatgtac cgataccttg atgtttaaat
    12721 ttttttcgta catagattcg ctcgatttct aaaaaatgat cagccatttc ctcagattga
    12781 ctcgttcctg tatttagttt caggtaaccc gcaggctctt gatcgatact cactaggaaa
    12841 aaatcggttt ctttatcttt tagctcttct gtaagttttt ctagtgcata agcttgtgtc
    12901 aagtaatcag ctaaatgctc cggtgaatta tcttttccaa atgtatctgt aaatgtttcg
    12961 atactaattt gttgtagtaa gtctacatct gaaggcgtca tttttcgaat ttcgtattta
    13021 ttcacggctt cttcctttca atagatcctt ttattgcctt ttttgacaaa attccaatct
    13081 tcgctaacat tcttgcatac tttatttaaa agatattcca attgttttgc ttcttcgtca
    13141 gaaagaccat ttaatgcgac tgcattggag tagttgtttt ctcgaatgat gaaaggatag
    13201 atttctgctc cttttttagt cggatagagt ttcttgattt ttttattttc tttatcttcc
    13261 aaacgttcga tcatcccatt ggattctaat ttcttgattg ctcgggcagt cgttgtacga
    13321 tctactttga tcatttctgc tagtttttct tggatgattc caggattttc gcaaatacgt
    13381 actaaataaa gatactgtcc tcttgttaaa tcgacttctt tgaattcgat attgctaata
    13441 gaatctagcg ctcgggcaat cattccgatg tctcgaagga tttcagccat ttcatcccac
    13501 ctttattttg tttgtaagac tagttcacct ctttttgttg caaatgcaac tattttcaat
    13561 aaaaaaatca agttcttttt taataactag taaagtatat tcttttcttc ttttacgaaa
    13621 aagctcaatc acaatagatc tggtttttgt tttctaaaat aagtaactgt ttcttcgttg
    13681 ttcgcttttc tgatagcttt ggcaactctt ctatactaaa aaatcccatg ttggaagttt
    13741 cattattctc aataaacttg gcctcttcac cgtggatcgc acaagcaaaa acaagtttat
    13801 aatactgaaa aagctgtgga atatcttttt gtttattcgt atcaaaaact gctcgcaact
    13861 gagtagtttc tactgttaat cctgtttctt catagacttc tttacgcaca ttttcttcag
    13921 gagacaatcc tatttcagca aatccaccag gaagtgacca ttctttcgtc cgtttatctt
    13981 cgattagtag gaccttgcca tcttttttga tataggcacg aacatctact tttggtgttg
    14041 gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc gaaagtttct tttcctattt
    14101 cactgatcag ttccaatgaa atcgtccgga tctcctcaaa acgctcttta tcaaatacat
    14161 cttttccata atatagacca gcttgagaaa gtgcgagtag tcttttgtat tgagccaact
    14221 tcttgttcat tctcttccct tcttttttag caaatgtatg actatgtaat caagtataga
    14281 agactggttt aattacggca aataaatata atgaaatctg caataaataa aagaatgtta
    14341 aaaaactcga gaacaaaaga cattcgactt ctctggggct gtgaataaat ggcgttcaaa
    14401 agcttgcatc tgcggtaata agccccacca aacgaaaaat atgacgagct attcttgtct
    14461 ggctgttctt attaattgag gcaggacagc tttttcacaa cctccccact aaacggtgtt
    14521 ctcctaccaa ctcctcggtc acaagtcaca atattcatca aacatgagaa gctgtttttt
    14581 tgaaattgct ccttgtgact cggagctgaa cgacaggctc acgacctctc ggtaaacagc
    14641 gttcaaaagc caatttttcg atattaagcc atttctttgt tacttattgt cattgatttc
    14701 tttttctaat ttatccaata gtggagtgta atccacttga gtatagtctg ttacatcaaa
    14761 ttcatatagt gttcctaact ggaatttgtc atattttcgg tcggcgatga tctttttgaa
    14821 tgcttctttc gtaatatgat tggtcccttt cgttcgatct tccaatacgt caccatagtg
    14881 ataatgatcc atgatatagc tcaaatgacg atctgattca cgatcacgtt tgtaccgacg
    14941 ttgccataac acttcaaagt ctgctgttag gcgaatcgtg atcacctgat atccgtatct
    15001 atctgctaat tcttggagtc gtgctttttg cttatcacta aaaggatact cggacacaat
    15061 cactttcttt cctgcttcca tatacaaatc aagtgctttg taatagaaag gataaacttt
    15121 cgtcctttct tgttccgccc gttcttccaa attgttaaac ccgatggatt ctgcatagta
    15181 ttccttgatt tcatcgagcg tgatggcata catatctgga aatcgttccc gtagtttatt
    15241 cataagatat gtttttcctg taccaggact accagccaga agaatgaaat actttttcat
    15301 ctcatttatt tcctaagtta attggacttt ccaagttcat aaacagtcca atcttatgct
    15361 tgacaatttc ttttactgct tcatttgctg gaatcacatt atggcgaaga gacttgttga
    15421 tttctgtttc actaaaggtt tctttagcta ctttcgtcca gttgactaaa agctccgttc
    15481 caacattgaa tttgcggatc ccgttattga tcaattcagg ataatcttct tctttcacac
    15541 ctgttcctcc atggataact agggggacat caactactgc atcgatttct ttcagcaagg
    15601 gaatgtttac ttctgttttt gatttgaatt gtccatgatt cgtaccgata gcaatagcta
    15661 atgcatccac gcctgtagct tctacaaatt tcactgcgtc ttccggtctt gtatatacct
    15721 tgtcattttc agcgacatgg attccttctt ctgtaccgcc gattgttcca agttctcctt
    15781 ctacggaaac accgtgcgca tgggcatatt ctactactgc ttttgtcttc aggatatttt
    15841 ctttgaaagg cagatgggac ccatcataca tgacagaagt atatccgctg tcgattgctt
    15901 ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa gactgcatca accatttctt
    15961 cttctgcaat agctgtcatt acattgacta acacatgcat gccgatatat ttagcggtat
    16021 cgacacttgt ttgaataatg atcggcgaac ccatttcttt tgctgcgcct agcatagcag
    16081 gaagcatttc taggttatgc gtattaaaag ctccaactgt aaaattcaac tcttctgctg
    16141 tttttgttac ttctttcaat gttgtataca tcttttattg ccaccttatc tttttatttg
    16201 tttcctttga tcgttacttg ttttgcaatt tgtcgcatct catccatttt gcccatgtag
    16261 taatcgatcc cttgatcatc accagcacga gcagcatctg cacgcttctg gttatagagg
    16321 ctcatcagtt ttttataccc atctccgatc gtctgtttat tttccaaact cttttctaaa
    16381 tattggatgg cttgatcgtt cacatacaat tcagaaaatt taagtccaac ttgttcataa
    16441 agtttcgcta atttatgctt atcttgggta gtggaaatct cttgtttcaa ttgtgtaatc
    16501 tctgattgaa tcttttcggt cgcttctggc gatagggctt tttcttcttt tgccagaact
    16561 tttttcggtt ctttttcttt tttcttaaaa aatccaaaca tcattttccc tactttcaat
    16621 taaaatgctt tcaaaactgg gcttaagatc caggcatata agagtgctgc ggctactgta
    16681 tctacatcgg ttgctgtagc attgttgaat cccatcgaat taaagatcgt tactagcaaa
    16741 gcgggcaata atgtaataaa gaaaccatga gcaataccgc caatgatcgc tcctcggcgt
    16801 ccgccaactg cgttgccaaa tatccctgct gtccctccgg caaagaaatt ggttagcata
    16861 ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa acatggcaat aatcgtacca
    16921 atcgttgtcg taatgaaacc taaaatgaca gcatttgggc tataaggaaa gaaaactgga
    16981 caatctaagg cagggatcgc atcaggaact aatttcatag ctatccctcg aaatgccggc
    17041 acgatttcac ctaacagtag acgtacacca gctagcaata catacacacc cactacgaat
    17101 tggatggctt gtaaaaaggc aaacatgata tagttttgat ctcctgataa ttcagaagcg
    17161 aatggttcgc ctgcaaataa aacagtgatg atatatagcg ggaccatcac gaccatgaca
    17221 gacaaataag tatcttgcag aaattcgaaa tgtgtcggca acttgatatc ttcgatcgac
    17281 tttttatttt cgcctttctc accaaaaagc ttagcaacac cggcttcaaa taagtacccg
    17341 atcgtacaaa aatgacctaa agcaatatca tttgatccag tgattttacg aatgatcggt
    17401 tgcgctactg ccggcatagc aatggcgaaa caagctccga taaaaccacc gaccaagatc
    17461 aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg tcgtcatcgt tgccatccat
    17521 aaaattgctt gaccagttaa aaaaatgtac ttccattttg tgaaacgagc caaaataata
    17581 ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc tacctaaacc caaatcattc
    17641 attgcttgac cattgataga ttcaatagaa gggatgatcc cttgcatatg aaatccttca
    17701 gtaaagattt tcccgaaata agttaaactt ccaacgatga tgctcgaacc agcactcaac
    17761 acttgaaatc ctaggagtgt ctttaacgtg ccagagatga cttgtcctgc tgatttcttc
    17821 tgcagtagta gacccaacat agcaatcaat gcaatggtga tcgatgcctg agtcaaaata
    17881 ttttcgatga taaaattgat aacgcccatg tttttaccct cttttctttt tgtatttaga
    17941 taactccctt atcttgcaga actggggtga ttttttcttc aatttcattt tttgaaacaa
    18001 tatttttcaa atagatcatc gccgttttag gatcaactgc gaacttttcg aactgcgaac
    18061 gaaaattttc tgctgtgatg atcacatctg gtttcatgga aactgcggaa gaaatatcac
    18121 aatggtccag tttggcttct actcctaatt tgttcaaaac atcctcagca gacatctgtg
    18181 ctgcaaaact acttcctaaa cctgcaccgc atacgaataa aatgttcaat ttgttcatct
    18241 ttgttctccc ccgtatcatt tattatttct caactactga aaaaagcagt tgtttgaaat
    18301 cttcgatatt tgtgcattca atcaatcgat ccaacttctc ttcgttgttg atcaattcaa
    18361 ttagttcttt catgatgttc agatgcgagt aggagtctac tgctgccaaa caaaaaataa
    18421 tctttactgg atccatttca ggattaccga aatcgattgg ttgctcaatc gtagctacgc
    18481 tgactcctaa tttattgacc ccatcttctg gtcgggcatg ggctagtgcc atgtgtttgc
    18541 caatcacgat atacggtccg taatcattca ctgcacgaat catcgcttcg acatagcgct
    18601 cttcgacaat gttttcttta accaatggtc tagcagctag ttcaatcgat tcctcccaag
    18661 tagtggcttt ttctttgatc atgatattgc tgtccgtcaa aatatctttt aacataggct
    18721 ggatctccct tttgttgatt tctaaattgt ttgtttcgaa taatttttct aacttgccat
    18781 agatgattcg atctactcgg ccaccacttt ctttcatgac atctattatt tgagtgaata
    18841 actccgtcga ttcatgactg ttaggctcta accgctgtag ttcttcattt cttttcaaaa
    18901 agtcttctat aaccaagata ttcgtctcag ttaaaatcgg atcgacaact aaaagcggct
    18961 tatttcgata attcaattga aaggtgctga aaaccagatt gacatccaat ttatctacta
    19021 attcgatttc ttttgaactt aaaactgcag taatctcgat ctgaggaaac ttttctttca
    19081 gattctctgc taacagattt ccagtagcca tcccatggtt acagacaacg actgatttat
    19141 aaacataatt caagtctcgc ttgattacac tggcgatcgt cgaaaagtgg atgacaagaa
    19201 aagctatctc gtcctccagt atcttgccac caattacttc ttcgatcgta ggaacaaaat
    19261 gagtgactgc gcggtagatt gggaaataat ttgttcggat gttttctttc aatggattca
    19321 tgacttggat atgattcttg attcttacaa tcagtgcagc catgtgctta tagagatttt
    19381 cgaataaagc ttcttccttt agatggaaag gtacgtgtgt ctcgttctct acaaattgga
    19441 tcaattggat cgttagtaat tgagcttgta cccactcgat tgaattggag atatctctag
    19501 cattgaacgt ctcaatagtg aaacgaagat aatttctttc tacttgcggg acattgtcca
    19561 attgaaattc ctcgatcatg tggtcaataa atgcagaaaa gctactgtct tttatttctg
    19621 tatttttcca attaatggca gagataaatt catgtttcct gattcgctgg atccagatca
    19681 atgtaaaaag caggatttgt tttttataaa tatcatcttc ttgtttcaac aacttattgg
    19741 aatagatctc ctgtattttt ttgatttcca ccaaaggaaa ataatggaga aagatttttt
    19801 gagccggtgt taattgtgca tcttgattca taaagaagtc ggtcattccc agattctgat
    19861 tgataaaatc atagatcatc gtacggatag atcgttctga acctttataa accagtcctt
    19921 gctttccata actgacgatc tctatcccat aactgaccag tgttgctcgt aaccgtctca
    19981 tatcttcatc taatgtactt ttagagattt gaaattcttc ttcttttttg tttagatgga
    20041 tcgggattcg gctgtaagca acggctaaaa ctagatcaaa aatacgttcg cgccgattca
    20101 aaaagccgtc tttcacatct tttatttcag tttctaatcg agcttgcttt tccttttcca
    20161 gcttcaacaa aaaacctttg ttgcgaattg tacggatctc cggaagcttt ttactgctca
    20221 accagatatt cgcttcaaga acttcctgac ggatcgtccg ctgactgaca ttgaactcac
    20281 ttgctagatg ttttgctttt actggatatt gggctgtcat gattctgtga agtatttcaa
    20341 ttgttcttgt tttcatagaa atgatctcct tctgatttca agtatatggt tccgcttaca
    20401 taataaaaag tagatatttc tgccacttga agtggaaaga aagtaagcaa agcaaacaaa
    20461 aaataagcca tatgcttttt catctcctca acgaaaaaga catacgccta ttattacaaa
    20521 aattttgccc atctatacaa aaaagaggtt gtgaaaaagc tgtcctgcat caagtaataa
    20581 gaacaactaa actaaaatag tgcttcctat ttttcgtttg gttgttctta ttatcgcaga
    20641 tgcaagcttt taaacaccat ttagtggaga ggttgtgaca gagatgttca gccttgagcg
    20701 ataagaagcg attttgaaaa atcgcttccc atattttaca aaatcgtgcc ttattgccga
    20761 taggctatct ctggcacacc gtttattcag aaaaaggagc tgcgacaagg cttttgtcac
    20821 aactccgttc ataaatattt cctgtcaatc cagcataatc ggctgaataa aatcggcttg
    20881 ccatcttttt tctttctcag aaaaatcaaa ataccatatc tgagaaaaat actgtcgttg
    20941 tgtcttactt cctgagatca atcgcccatc ttttcgatcg actgaataat cacagcatct
    21001 gaaatcaatt cggatggcaa aactattttc gtggatctgt cgataatttc ctacttcctg
    21061 aatagttatt tttttagtat gatttcttac accgtctgct ttgttttttt gcagaacttt
    21121 ctgatgttcg gcaaacagct tgtctgtata acagcttctg gctttttcag gagtttcttc
    21181 ttcccaagcg gattggatct tcatgaatat cttttggatt tccagaaact gtgctttctt
    21241 ttctttagcc gttcctgaca tgcgggacaa tatctccgct ttcagccttt tcttttgtct
    21301 tttattcttg acgatagaaa aagcagaaac ccctcctact gacaaaacag ctaatgcaag
    21361 aaatcccgtt tgctcataac tagaaggacc tgtacctgga ccataatacc cataagaatc
    21421 tctgccgtaa gaacgactgc caaaataact gcccccatat gaacgaccac caaatgaacc
    21481 tctgcccgaa cttcgaccgc tagatgtacc tccaccatga ccacccgcga cagcgaatcc
    21541 ttgggtcggc agagcaaaca aacataggcc cactaacaaa atgattgcta aataattgat
    21601 ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa ctatttttat gatagccttg
    21661 ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt tttagcaatt ttgtcttatt
    21721 tagtcattga gtaacatcgg acaaatgtat gtgtcggcag tccataattc ctctatcttc
    21781 agcatgaaca agcttgcaga caagagatct gcgcttcctc caggagagat ttggttttcc
    21841 tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta aagcactttg actagcagca
    21901 tgagcaagtt tttgcatttc cactaacgtt ttctgatcac tgcgatgcaa tacgcatgtg
    21961 tcatctagcg aactcatcaa aactaacaac atatgcattt gtttctgtac atatgaatca
    22021 ttatctagct ccttagctgc ttgaattgca agcctgatat gcggataccc taatatcgct
    22081 tcttcatatg caccatttac gccatatttt tgcttcaccc tatgaccatg agtggttttt
    22141 ttatgacgat attttgtatc aggaaaagca gcaagcttgc tgacatcatc aagtattttc
    22201 gataaagaaa aaggctgttc catactgatt ttttgtgcaa tgacactgat caatagcccc
    22261 attgcccaaa tcgctccttt atgggtattg atgccatttg tagcaatcag cattgccttt
    22321 tccgcttttc gcccaatctc agcaattctt tctcttaact cctgatcaat agcatgatgc
    22381 catgctattt gagccatttc ttcaaaaaaa ggagttagcg tcttagcaga cttaagaaat
    22441 aaagcatagt ccatatcgga atgtgcaccc gcatccagtg catccactaa tccaggtttt
    22501 ggacttaatg ttacttctgc gatcaatgct tcctctacca acgcagcgag ttgctgagtc
    22561 actgcttttt ttcgttcgtc atttatcatg acactcactc cttcttccta ctattacttg
    22621 ctgaaacctt ttttgatagc tacccaaatg atatgaagta accagcttaa agcaaatgca
    22681 caacttgccg tcattagata agctacaaga atcatgactc ctgaaggtaa ctgcagccaa
    22741 ctatctttaa acaataataa cagtacttgg aaaacaaata cattggctaa atacgtacga
    22801 taagcataaa cagacaacca tttgaacaat ggcaattttg gcgaatcgtt tacaatcatt
    22861 ttggaagcgc ctatgaagac agcaaagatg atgactaaac tgtataaact ttggatcgta
    22921 ttcaagtaag gtgcattcgc aaagctcaag tctcctggat gtgcgagcaa tgaacggaca
    22981 gaaaagaacg cgataactaa ggcaacaggt aagaaaagat aacgtactct gtccaaatat
    23041 tgatagactt tttcgtgata aacaaatgcg gctaccccat aaatcatgaa actagcaaca
    23101 aaccgatcta ataagtacca gctgtcatga taaggcccag tcaatacttg tgtttgatag
    23161 aacagatacc atgcgacata taaaatcgtt gaaccaatca atactggcca tacatatttt
    23221 ttattagcaa atactttgta accgagccaa acaaaaaacg gcatgaacaa ttggatctgc
    23281 agcatcataa cggtatacca taaatgaggc gctccatcac ctgtgacata tttcagtaaa
    23341 aagcttgcag tatccgtata cggagtttgt tgttggactg ctggaaatag ccaaagataa
    23401 gccgtcgtcc atagaagata cggaacgact agttcaaaaa acttttcttt caaaaattcc
    23461 caataagaca aatgctgact tgtttttacc atattataaa caattgcaaa aataaacatt
    23521 ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa aagctgtatt tgttgcgctg
    23581 tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca taacagccgt tacagctgat
    23641 actttctgga agtcacttac gttgattttg atttttttgt tttcaaacat ctctttctct
    23701 ctctttttct ttattaccaa agttctttgg ttaatttagg tccttcgact gttttcaata
    23761 gaattttttt ctctggctct ctcaaatact cttttagtga aaaaccgcct tgccgagtca
    23821 ctacttgggt atcgaattgc tcaaaaaaag caggatgcga ttgaattacc tgaattggca
    23881 attcaatcgg tgaatttgcg tacagcagca aatcaaaatc actggtttct tttactgccg
    23941 gaatcccagt cgataattca aatcctaagc tgccgccgat tccccattta catcctgata
    24001 atatgtttct agcagcatga aattgctgat aaacaggata atccatgatt ttctcttgtc
    24061 tatacgattc ttgttcgatg atgtcccacg gatgaatgac ttgtttgacg gcatgttttg
    24121 agatttctgt tgaccatctc tgattgcggg caggtccacg taaaccaatc ggtagtattt
    24181 ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat cttttcagtt agaacgaccc
    24241 attcaggcaa ggtgaaccct tctatttcat gtttatcaaa aaggacaata tcatgtgctt
    24301 gaatcatttt ttatgcatcc cattcttcgt ccattttggc acggacttta tttgtttcga
    24361 cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt tgtcagcatc gtgtcatttg
    24421 tattgcggac atcttgaatg gcttcattga ttgcttcgat gactttgctg acattttgtt
    24481 ctgaagcagc ttggtcggtg atatcttcta ggaaatgata gatcgctcct agttttgtat
    24541 aattattgat atcgtaagcg atagctggaa ctttttcagc agctgcttcg atttcagcaa
    24601 ccgaacgttt tgtcacacgt gcagcacttt ctttagacat cgcttggacg gtaatttctt
    24661 tattgtttaa agcaatcaag cggcttgatt gtaatccatg tgctaaaaat gcacctgaaa
    24721 tagcatttcc tgcaatcaat ccaataactg gatgtccagc ttgacggagt tttgcataag
    24781 cagcagcact attagccaaa gccacatgga taccgatcaa ttcttcgtta tagccgtatg
    24841 cttggcttgg tacatcgatc acaacgatga ttggccgttt cacttttttg tctttatctt
    24901 cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc ttcaactaaa ccaacttcac
    24961 cgtttcgtac acggtacatc gggtttttag gatcaggtac gattgatgtc acgatggctt
    25021 cttctatgaa tttacttgat ttaccataat acacagtact aatagaagaa gttggatttt
    25081 tcatgccagt tagtgccgca aaccaatcgt aaccaacact tgctgcagct ggttcatttc
    25141 cttctgctac ttctggtaag tcaattgatt ttgcttgatg gttagcaatt gcttcattgt
    25201 aatcgtcaat ggccattggt tttgaaagat ctaaagaatc aagtaatgat aaataaaatg
    25261 ctgagctttc actgcgatgc gtgtcttttc gttcgtccat tgctgcgatg acagcagctt
    25321 tgattgcttc tacactatca gtcactaatt catcaatgat ttttgattct acccgttgac
    25381 gagatcctaa tgtattccaa atcaagtctt tgtcactaga atcaaattct cgaacgcctg
    25441 cttcttgttc aataacttct ggtccattca aaccgactct cgcttttttt gttgcgatta
    25501 aataactcat taatgctgaa gtaatcgaca taccgccaaa tgatcctaca cgtcctggaa
    25561 tcaaaccgat gactggtaca tattgtttta gagcaatgat tacatttccg atttctgaaa
    25621 tagataacag tccgtagttt gcttcttgca aacgaacacc acctgtatct agtacaatga
    25681 ctggaaagat ctcatttcct tttttatttt cttctaacac atgttcaagt gcagcaacga
    25741 tttttgcacc ggaaacttca ccgataccgc caccttggaa ttttccttcc atagcgatta
    25801 ctactgcttg tttgccattg atcgtgccac ggctgacaac gatcccgtca tcactttcag
    25861 ggacgattcc ttgtgctggt aaacttggcg cgatgatttg atcgaatgga tcaatcaatt
    25921 cacgtccatt gtcaagtaga gcgttcactc gttctctggc gtttaattct acaaaactat
    25981 ttttcatctg aaagttcctc cattgcttgc gtcaatcgta ggtacacaac tcctggtgta
    26041 gcaccgaagt cattgatcac gatatctgct gaaattggat aacgatcaaa aaatctcgtt
    26101 aatacattgt tccatacttc gccgaagcca tcgcttcctg tacaaatttt tacagtagat
    26161 tctgtttttt ctgttggaaa catcaagatt tccaaatctc ctgaaccgac cactcctaca
    26221 tggattttct tattgatcgg ctgtttcgtt ggatatttat aagctaattg ttccatggat
    26281 ctctcccctt tttgtaaaac agagcataca cgttctgctt attcgtcttt cttcttccat
    26341 ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta tgaccaagtt ctgaatttca
    26401 ttggtggttc atacaggcca tctgaccatt cgatcaaatc atcgatggtt tgtgcagcta
    26461 ataatgaacg tttcgcttca ctacgtttga tgcctagatc ttctggtaat ttcacgatgc
    26521 cggctgcacg caagctatct agtgattctt tcgtgctagt cattccaaga ggtgtaacac
    26581 cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt ttttgctttg tacaaataag
    26641 caattccttc ttctgtcacg atatgtgtag tatcttcgct ataaatcatc actggtgcat
    26701 tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa ttcttcgaca aagactggtt
    26761 ttttatttga accatatgtt tcaaccattt gaacaactaa tttttggcct ttccctaatg
    26821 gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga atgacgacga ccgccaggat
    26881 tatgtcccat gttaggcgca ccgccaaaac cagataaacg tccttttgtt acggtagaag
    26941 agtttccttc gtaatctaat tgcaaggttg aaccgataaa catatcgatg gcatattgtc
    27001 ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc gtctttaccg acaaaaaaga
    27061 catctgggcg agcagcaata tatttttcca taccaacttc gccaccaaaa ctatggatgc
    27121 tttcaaccca acctgattcg atggctggaa tcatcgttgg atgtgggttc aatacccagt
    27181 tcttcgcgat tttccctttt aaccctagtg attcaccata agttggcagt aacaattcga
    27241 tagctgctgt attgaatcca ataccgtgat tcaaagattg tacgccgtgt tctgcgtaga
    27301 ttcctttgat tgccatcatt gccattagaa tttgcaattc tgtgatgttt tgaggatctc
    27361 gtgtgaataa agcttctaat tgataaggtt tatctgcttt cacgacaacg tctacccagt
    27421 caccagggat atctacacgt ggtaaagaat cgacaacttc gttaacttga acgatcacga
    27481 taccatcttt gaaagctgca gcttcgatga ttgttggtgt ttcttccgta ttgaaacctg
    27541 tgtacaagtt gccgtcttta tctgctttat ctgcagctac taaaacaaca tttggaatca
    27601 aatcgatgaa taagcgtcca taaagttcca aataagtatg gatatcacct aatttcattg
    27661 tgccgtcttc aatcatttgg gcgacacgca agctttgtgc accagcataa gagaaatcga
    27721 tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc tggtcgtgag atactagaca
    27781 tgatcaaatg cagatcattc actttttctg catctacttt ttcaagactt tctgaaagaa
    27841 aacttgcttg tttttgattg tcgccttcta atacaacttt gtcgccggga gcaatcagtt
    27901 tttccattac ttctacgatt ttttcagttt ctacatactt gccattgagc aagttgctga
    27961 ttttttccaa tcgctgcgct tttttttcgc gtttagaagc ccagttgcgg tttggttttt
    28021 ccataattag tcatctccat ttttgatata gaaacgtggc gtcatcaata ccatgttggt
    28081 ctagattgat cgtacggatc cctttatcta caccaaattt tgtatgaatc agctttgtca
    28141 aagtattgcc ggggggaaat tcaatcgtca gatccattcc taattctttt gccaagtcca
    28201 tcatttggtt ccactgcaca gggaaagcta ggttttccaa caaatcttta cgaatctctt
    28261 ctgcgtcctg agtggctctt ccatccgtat tttttatata caaacatcta gcatcagata
    28321 gatggacaga ctgcatatac ggcttgaatt tcttgaccgt atcattcatc aagagacagt
    28381 gagatggcac agggactttc agcagcttcg ctgtttgcgc ttggtgccgt ttggcttctg
    28441 ccaacgtaac ggataaacct tcgatactcc cagataacgt atgttgcatc ggacaatttt
    28501 catttgacag ataaactgga tggtcaggat caaatgtttt tgctacaatt tcctcagctt
    28561 cgcttctcgt cacacctact actacgccca ttgcataacc gcttggataa gcttctttca
    28621 ttaagactgc gcggtgatag actagttccg ctgcattctc aaatctcaaa gatcgacaag
    28681 caacagctgc acaaaaagca ccaatcgaat gtcctgctgc aatatcaggc tgaataccgt
    28741 ttttttccat ttcatctaaa taaaatactg cttttgtcag taatgctagc tggataaaaa
    28801 ccgtatcttg atagttttct tctttatcaa ctaaccgata ccccgtcaca ttttctaaac
    28861 gttccatgta catttcaggg atatccgcta acatccctaa cttctgactg ccttgtccag
    28921 gaaataagaa acctgtactc atgagcctcg cctcctcagc acttactttt tccactgcta
    28981 tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa gaaaaaacca taacaaaaag
    29041 gtatggatgg gatggataat tatgaaattg cttcatctgc agtattttaa aaaagtattc
    29101 gaaacaaaaa atgttaccca agcagccaaa gaattattta tttctcaacc tgccttgagc
    29161 cgtgcgatca aacatttgga aaacgaatta ggtgtccctt tgttttacca tagcggcaga
    29221 aacattgaga cgaccgttta tgcagaagaa ttttatccat atgcagtcaa gacattagac
    29281 acattagaag aaggaatcaa attattgtct tcggtcaatg agaagatcgt tacttccgtc
    29341 atattgtatt tagaggtagc ctctgtttct attccgaact tggtccgaat ttttatggaa
    29401 aagcatcctg atatccaatt aacgatcatg caacatcatt taccagatga tattaccgat
    29461 cctgtactgt acatcacttc ggagatgaag cctggcatga ccaatattcc tatcatgaaa
    29521 gaacctgttt atgtcgccat tcctaagaaa catcctcttg ctaaaaaaga acatctgcaa
    29581 ttagaagata tccaacgcac ccccatcttg atgctgtcaa agagcaacgc cttccgtcat
    29641 acactggatg cagcacttga agcgaaaagt atcgaattga cgatcagttc gacgacagat
    29701 gatcctgcga cattacgttc tattgtcaag caaggattag ctgttagctt tttcccaaaa
    29761 gtttcttggt cttacgataa aaatgatcct ttcgtcttgc gggaaatcaa agatttgccc
    29821 ttgacacgaa caatttattt atcttctagc ttcacagaag atcatccttt gatcaaaatg
    29881 attgcaaaaa cgatgcggga attttattta ggaaaataaa aaaaaaagag ctgggacaaa
    29941 attcttgtcg cagctctttt ttcgaacaaa cggtgttcca gaagtaattt cttagaaaat
    30001 aagccgaaat ttcccaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
    30061 agctaaacat ttctgtcaca acctcttctc tattgtccta gacattcatt gcgttgattt
    30121 aaaacttcca tcaatcaaat ctgctagttc atctagcttt tctattttac gtagatcaat
    30181 agagatccaa tgttctttat tcatgtgata ggctggaaaa tacccctctt ttcctcgcaa
    30241 aattccgcca agttcaggat cgaccttcaa atcaagaata ttgatgatcc tatggtcact
    30301 tgcatcatca tagatttttt cttctggaac agacatcagg accccaaacc attttttgct
    30361 tttttgatgt ctgaatacgc aataatcagg aaatttttta aaaatatatt ctggagacaa
    30421 attgtatgtt tcattgatgt gatcgattag ttcttttttc gttagcatct gcttccatct
    30481 ctttcttatt gattcgcttt attcaaagat accacgtaag aatagaaaaa tgaatacaag
    30541 tagttgctaa tcaaatactt cgatattttt cttttttatc aactcttaaa tgttcaatcc
    30601 gcctgtgttg gtcttgctac catctactat taaaatgttt attattgttt gcccacctat
    30661 tttttgattt tagtggtatc atttttcaag ttcatgatga aattcttatc atcagtaaat
    30721 gctccagaaa aataaacaat gattgaaaaa tcagttgaaa cttgaccacc gtttattttc
    30781 tccgtattct tagaatagaa gggagtctga ttgatgtatt cactaatgaa aaaaatcatt
    30841 accgaaaaag atattcgacg tcatgtttct ttagtagaac aattgctgaa tcatacaaag
    30901 ataaccgtaa atgaattagc tgaaatcata gggacaaccg aacgaacgat tttttctgac
    30961 cttcaatcta ttcgctctca gctgcccgaa ggctgggaca tcatcagcga tcaagcagga
    31021 atcagtttgc aaaaccagca aaatttatta acaaatgacc tttgggagat ttttttcaaa
    31081 caatcagtca gtgtcgaact gctgaaaaat ctgcttttta caaaaaaagt tgctgtccct
    31141 gattttctag ccgaccatgg gctttcttat gggacattga aaagacatgt gactaaaatc
    31201 aatcagcgtt tagcaagtta cgacttgcag atcgatttga cgaaatatac agcttgtatt
    31261 cttggaaaag agcgtgcgat tcgtactttt tatcatcgat tgctgattcc tttcactcat
    31321 aacaactatt ttttcgagga ttattcgatc catgaatccc attattttca atttttaaga
    31381 aacctcagtc aaactgaact agcggttgaa acagaagaga ttttcggaac atgttggttc
    31441 tttatcaata cgatccgtat aaaagcgaat tgccgattag attcttctat ccataccaac
    31501 agcacactct cctccctgta tgactctgca ttgaaaaaat tgtatttaac ggaaggtatt
    31561 tatcttaaag acactgagct ttcttttgct tctttctgct ttctggaaag ttggaattac
    31621 aacaataact atggacaaga aaccgctcgc tgcctgcatc attctccatt tttagaggtc
    31681 ctcgaaacgt ttgtagagga actagcttca gagctgtctc ttgatcaact aaaaaaaaca
    31741 tctctgactg ataacttggc tctcctcata ttaaaatatc atgaatcgcc gatattgatt
    31801 gaagcattgg accgtcaata ccatcgtttt ttagaaacat atgatcagca atatgcacac
    31861 ttgtatcaac agaaagagaa tttactgaac gatctaaaaa aagaaataga aatcaatgaa
    31921 ccaaattact ttctccaact cttgtctcta ctgatccaac aaacgatttt ttctatcaaa
    31981 ccgaatttgt tcaatgtcta ttttattttc caaggagaac cttcatggaa ggcgttcttg
    32041 cagcaggaat taaaagatta cttaggaaaa cgtgtctgct tcttacctat cgaactaacc
    32101 gatctttcca ctatttcttt tgaaaaaaca gatatcttga tttccaattt ccctctagat
    32161 tctctagata tccctattgt atacatttct tctattccaa cgaaaaatga gctcaatcgt
    32221 ttgactgaac tcactttcaa aactttttta tagtaacggc tagcgactgt attctctagc
    32281 tgttttttct tttttgcgaa acagtttgtt catgatccct ttttctattt ctacaactaa
    32341 gaatagaaga atcacatttg ctccaatcaa taactgttgt gtcatcgtta aacttactgt
    32401 accgatcagc tgctggccga ctggtagaaa aataaccatc ccttgcaata aagcaaggat
    32461 acctaatgaa agaaaaagcg ctttgttttg caaaagacct tggttcaatg acggcttgga
    32521 caactctctg caattgatca tgtaaacagc ttgagctaag acgatatttt gcagaagaat
    32581 ggtttgctgt aagctttgcc cttcaaactg catagccaat aaataggcag gaaccatgat
    32641 cagtagggag acatagatga tccgaaacaa atgataacca gataggattc cttgctttgg
    32701 atctcttggt gggcgcttca tgatttctgg atctggcgac tcgaacccta gtgcataaga
    32761 taaagtgatt gtcgttacca tattgaccca taaaatctgt actggggaca aaggtagcgg
    32821 gcgattcagc ataagtgccc aaatcaagat caatccttga gctaatgcag ttggcaagaa
    32881 gaaattgatg gttttttgca aattatccaa aatccgccgg ccctctttta cggcttttgc
    32941 aatcgtatgg aaattatcat ctcctaaaac catatcagct gcctgtttgc ttacttcact
    33001 acctttgatt cccatagcaa ttcctacatc ggatcttttc aaagcaggtg catcatttac
    33061 gccgtctccg gtcattccga cgatttctcc atttttctgc aaagctttaa caattcgcag
    33121 tttatgttct ggtgtcgttc gagcaaagac atcgactatt ggcacttgct gaatcaactc
    33181 ttcatcagac ataagatcca aatcgatccc ttctaaaact ttggcagtat gttttaagcc
    33241 aatttgttcc gcaatcgctt ttgctgtgtc tttgtgatcc cctgtaatca ttttcactga
    33301 gatccctgct tgttggcatt cctttacagc tttgactgca ctttcttttg gcggatcgat
    33361 caatccggcg atccctgcaa aagttaattg ggttaaagtc tcatgcgtga gttcctgttt
    33421 cgaatctact tttttataag caaatcctaa aacacgctga ccttttctcg caaattctgc
    33481 agctgtctga ctccatgctt cttgttctgc agaagaaaga gttgatttag caaataatac
    33541 ttctggtgca cctttgacgt aaatcaccgc tcctttttcc tccgcatgca tcgttgccat
    33601 atatttgtat tcagaactga acggaatctt tgcttctatt ttttcaaagg tagattggct
    33661 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
    33721 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
    33781 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
    33841 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
    33901 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
    33961 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaagaat atgccaatcc
    34021 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
    34081 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
    34141 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
    34201 ccgactgcag aaccagattg tacaagcgtt cctgaaaaac ccatattttt ctggtcgccg
    34261 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
    34321 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
    34381 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
    34441 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
    34501 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
    34561 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
    34621 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
    34681 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
    34741 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
    34801 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
    34861 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
    34921 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
    34981 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
    35041 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
    35101 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
    35161 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
    35221 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
    35281 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
    35341 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
    35401 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
    35461 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
    35521 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
    35581 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
    35641 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
    35701 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
    35761 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
    35821 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
    35881 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
    35941 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
    36001 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
    36061 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
    36121 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
    36181 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
    36241 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
    36301 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
    36361 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
    36421 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
    36481 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
    36541 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
    36601 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
    36661 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
    36721 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
    36781 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
    36841 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
    36901 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
    36961 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
    37021 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
    37081 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
    37141 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
    37201 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
    37261 gccatatcaa atttcaaatt caaaaaagtg atgatccaat aaacgactaa aatgacaact
    37321 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
    37381 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
    37441 acaacggacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
    37501 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
    37561 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
    37621 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
    37681 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
    37741 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
    37801 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
    37861 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
    37921 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
    37981 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
    38041 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
    38101 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
    38161 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
    38221 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
    38281 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
    38341 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
    38401 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
    38461 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
    38521 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
    38581 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
    38641 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
    38701 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
    38761 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
    38821 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
    38881 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
    38941 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
    39001 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
    39061 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
    39121 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
    39181 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
    39241 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
    39301 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
    39361 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
    39421 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
    39481 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
    39541 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
    39601 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
    39661 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
    39721 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
    39781 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
    39841 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
    39901 acctcttcac tatagcgcca aaaatgagaa tttttttttt ataacatcaa atccacgtag
    39961 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
    40021 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa
    40081 ataaaatgac tgtccttcat tcactaaaca tatttttatt tttttatatt gttaaaaatt
    40141 cgcaatttat tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt
    40201 tttaacggca tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt
    40261 cataatattt cccatattgt gcaaaaaaat acaaggcgga ggctctacta tgattgaaga
    40321 atatattgag aaaaatattt taagacagct gtttttatgt gggcaattct atgtaaataa
    40381 agaagtcaat ctagaaaagc tctccaatct cctgcatgtt tgcaaaacca ctttactcaa
    40441 tgatatcaat aacataaaaa aagaatttga agaacagatc gcttatacac atcgggaaaa
    40501 ggaccgttac actttatatt tttcagaaca tatacctcga tgcaagatca tgcagcaatt
    40561 gtctcaaaat tcgttgtttt taaaaacttg tctgctttat ttggaagaag atgaacctga
    40621 ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa
    40681 acaagtatta gcttatttta atgactgcgg gatcgaaatc gaccgctact cccctcgatt
    40741 cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg tatcgtttag gcggttttaa
    40801 ttcttgggag ctacctgaat cgttttttga acgtgccgat cgtttcattg aatccgtaac
    40861 ggaaaatagt ggcagatttt atgataaaga aaataaagaa atcttatcga ttggttttgc
    40921 tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga
    40981 aatcaaaaaa cgtcctattt acaattatgt cgaatcagca tgggaaaaca ctgatttcca
    41041 aacctattat aaaaaagaag aattcgcctt tattttgaca ctattcaatc tttgtaatta
    41101 tggtttccat tcttatcagt tgattgcgga agactttcaa cagctacacc aagtgtttat
    41161 tgacaatact cccgaaataa aagaactagt tgctaccttt gaatctcact tcaatcaaga
    41221 attattcgga aaccaaccgt tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga
    41281 taactatcaa ttattcatgc cagaaaaatt ttatctttta aatgaggaac agacaaattt
    41341 atataaagaa gtccagacaa ttttttccag ctggtccagc cagttacctt atgatctacg
    41401 gttgaatcct aattgcatgc gggcttttgt gatcgaattg agcggaattc tccgtctgac
    41461 aaaagaacat ctgactatct atatcgtcac aaattcggat gttcattatc tgatttatcg
    41521 ggaagccttg gaagctgtaa ctactttcga ttttcaagtt gcaccaacaa tttattcaag
    41581 tatatctgat ataaagaagt atgcgcagca gtcctcaaac cgggtattat gtgaacgtac
    41641 attatataca ccagatgcgg tccaatatga aaatattatt cctatttcga tcaatacgat
    41701 cgatcgagct atcatttcag ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc
    41761 acagcctctt atttgttgaa gatgcccatt tctgtaacaa tctcttgata cacgtcaggc
    41821 tcacaacctc acttattttg gacatctacg ggtccgatct ctttatttaa aaaataagaa
    41881 atgatggttc ggataactac caacgccgct agttggaaaa tatcctgcaa ggtaggtttg
    41941 ataatcgatt cgatgatccc agctgaaaca agaatttcca aacttagaag tatatagcct
    42001 cccaacatct ttttaatagc attgttctcg ctgactcggt cgctttgcac ttttgtatgg
    42061 acaaataaat atttcaaaaa atccttgatt gatagacaaa caccccaaac caataaacag
    42121 atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat atggaagaag aaactgcatc
    42181 aactctgtcg tcatcctacc tctcctcttc tttgtttttc tttgtcttct atttcctgat
    42241 tcaagaaata agaaatgact gttcgaatga taataataag tgcaagcttc aaaatatctg
    42301 tccaagtcgg cttgataact gactcgatga tatcagcaac gactagtact tccaagctta
    42361 acaaaatata acttgctagc agttttcttg tatctgcatt acgagtatta cgttctgcat
    42421 agtttttctt gacatgagac caatcaaaaa gtttgaatag aacaagaacg atcccgacaa
    42481 ttaagatacc gatagaacaa atatttaaaa tagcaatgat ccattctaga aattcaatta
    42541 aatttgagcg aaacatatac gctacctact ttcttttaat ttgttcctat tcttttattg
    42601 taaaagaaag ttaccttgtt cgtctattta aaagttgttc taatgataac ggttcgctta
    42661 aatttttctt tttcactctc ccatttttct ccctccatcc ataaatcgtg gaagtatcca
    42721 aaacaacgta gcaagttaaa ataaggctta gtccgtcatc agcttttcaa agaagcactg
    42781 tctcggtgct ttttttgtac ccaaatttct aaaaatagaa ccatacacct cctcaaatct
    42841 tttcttctta cctaccctat ttttatttac aggaaaatca gacacttctt cttttttgtc
    42901 aaaatttcct ttttcttctc gtcctatcat ttggcattct aataaaaacg aactatcctt
    42961 ggatatagaa agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg
    43021 catcggttta ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaga caggactatt
    43081 cgaagatgac agtcatttat atgatgaatt cgaatcaatc tagcatccta tttagttgaa
    43141 tcactacgga catacttgga agacattgga ggaatcacaa tgagaaaaag taaagcaata
    43201 atgattggtg ccggtattgc caatatggca gcggctgttt atttgattca agaaggcaaa
    43261 tggaaagggg atcagatcac cttttattca ttggatgatc acggttcaaa tgatggtgca
    43321 ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc atccattaga aaaccaaaaa
    43381 ggctatattg ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga tttgatggat
    43441 ctgttaagtc gaataccttc tgttactgag ccagggatga ctgctgaaga agatacgaga
    43501 gattttgatg caaaacaccg aacctttgac aaagcacgtt tgatggaagg tggcaaagga
    43561 atcattgatg gcggaaaact gggattcaat aataaagacc gtattcttct gactaaactg
    43621 attgctatgc ctgattcaga agaagaaaaa ctagacaata tcactatcga agaatatttc
    43681 aaagatgatc cacacttttt tgaaacaaac ttctggttta tgtgggaaac aacatttgct
    43741 ttcagaacac gtagttctgc tcaagaattg cgtcgctata tgcatcaaat gatttatgaa
    43801 ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac gttacaatca atacgaaagt
    43861 attatgcttc ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc
    43921 cgtgtcactg actgggaatt caaagaaaca ccgatgcaag atgaaatcac tgtgactggt
    43981 ttgaaaatta ccaatacact gactcaagaa gaagaatacg ttgtggtcga tgaagataca
    44041 gcagttattt tcactaatgg gtccattaca gattcagcta ctttaggaga ttacgaaaca
    44101 ccagctgtag aaaatatgga ttatggtgca gcttctagtc tctggaaaaa agccagtgaa
    44161 cgtttctaca acttgggaaa tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg
    44221 gtcagcttta ccctgacgac gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg
    44281 actcaagaac caggaaatgc cttgaattca tttatttcaa ccacaccgat cacgccgtta
    44341 ggccaaaaag atgtcaatat gtctatcgtc gtgcatcatc aaccgcattt tacgtctcag
    44401 aaaccaaatg aaacagttat ttggggttat ttcctttatc caagacgacg tggcgaattt
    44461 gtggataaac agtatattaa aatgaatgga aaagaaatgc tagaagaact gattggacaa
    44521 ctttcaaaag tagatcctgg accagtgaat attcgtgaaa aagaaacaga aattttcgac
    44581 agcgtaataa ataatatccc tgtctatatg ccttatgctt ccgcgttgtt caataatcgt
    44641 gccaaaagcg atcgaccaaa agtcattcct aaacattcga ccaatctggc ctttacagga
    44701 gaatttgtcg agcagcctta tcagatgatc tttactgaac aaagtgctgt acgttctgga
    44761 gaaatcgctg ctttccattt tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt
    44821 tacgacaaag atattcctac tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga
    44881 ggttgtgaaa aagctgccct gcatcaagta ataagaacaa ccaggctaaa acagcttctt
    44941 atgtttttga tttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttacca
    45001 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cacccaagct aaaactgctt
    45061 cttatgtttt tgacttggtt ggacttatta ccgcagatgc aagcttttga acaccattta
    45121 ccaagaggtt atgaaaaagc tgccctgcat caagtaataa gagcagccaa gctaaaacag
    45181 cttcttatgt ttttgacttg gttagactta ttaccgcaga tgcaagcttt tgaacaccgt
    45241 ttaccaagag gttatgaaaa aggcgtttag tttcgagtat gaagaaaaat tttgtgaaaa
    45301 tggttcctca cgtttttgca taaatttgac ttaataccga gaaactgatc ccttctgtca
    45361 taacctcttt ttatttatgt ctgtttcttt ttgaaaacaa ctattttttc aacgtttact
    45421 ctcttgccaa tccaacatca tctaacggat acaactattt caatgattac cattacacta
    45481 aaaaatcgga aacacacctc tccgaacaca aatacataag actatagaat gtttcattta
    45541 agaaaaaaag cgtttcatta tcaaaaaggt aagatctcct ttaaaatgaa attatctgta
    45601 gtactaataa tgcagacaat tttatgaatc tgaaaggagc atctccaatg aaagcagtca
    45661 cttggcaagg aaaacagaaa atggaaatca gaaacgtaga tgatccaaaa atattagaac
    45721 caacagatgc aatcatccgt atcacagcaa cagccatttg cggctccgat ctgcatcttt
    45781 accatcatgg tgaagcagtt ttggaaaaag ggtatgttgt aggacacgaa ccaatgggaa
    45841 tcgttgaaga agtgggatct caagttcaaa cattgaaaaa agggcaacgt gtggttatcc
    45901 cttttaatat tagttgtggt cattgtcatt tctgcctgaa tcacatggaa agccaatgtg
    45961 atgaatcaaa tccagatcag ttatatggcg ggcttttcgg atttggcaag ttgaacggta
    46021 atcattgggg tggtcaagcg gaatatctaa gagttcctta tgcagactct acttcatttg
    46081 ttgtaccaga taatgatctt cctgatgaaa aagttttatt tctatcagat attttgccca
    46141 cagcttattg gagcgttgaa aacgcaggag taaaaaaagg cgatacagtt gtgattctag
    46201 gttctggtcc agtcggtcta ttcgctcaaa aattcgctgt tatggctgga gcagagcgcg
    46261 taatcgcagt agatccagta cagcaccgtc tagataaagc ggctaattac aatggggttg
    46321 aaacttattt cttagaagac acggcacaag ctggaacaga attgtatgaa ctggctaaag
    46381 gtggcgcaga tgtcattatc gattgtgtag gaatggacgg attagagcct gtcaaagaaa
    46441 aagcgaaaaa tttagtaagc ttacaaagcg gcacaatctc tccattacag atggcatcgc
    46501 aagctgtcaa aaaatttgga acagtccaag taactggtgt ttatatgact ccagcttctt
    46561 cttacccgct gcaagaattt tttatgcggg atatcgctgt caagcatgga caggcaccaa
    46621 ttatccatct aatgccaaaa atttacgcaa tgattgcaga aggactattc gatcctacac
    46681 aaatcattac tcattcgctg ccactatcag aagcagcaca agcatatgag atctttgaca
    46741 aaaaagaaga taaaaatata aaagttgttt tgaaaccttg aattcagtaa aaaatacagg
    46801 tgattttctt tatgcccaaa atcaaacgaa atacccatga gatcgaaata ggaaaagaag
    46861 aagatatcga aatacgagga cgtcgcttcc gcttctattt ccaaaatcgt tacacattga
    46921 tctcactggc tgtcgattta ttaaccggga ttttctatat ttttggaagc cttgcttcct
    46981 taactaaaat ccccgatcga tatggcacct atttttattt ggctggtgct attttcttga
    47041 cgattcgtcc tattctcaga atcgtaagga acatttttat ttacgacgaa aaaaaagcaa
    47101 tgaagagaac aagcaaaaaa aaagaggcat gagaattttt caaatctcat gcctcttttt
    47161 tcaccctatc gtatcacttt gatacagacg agcggtcgtc ccactttctt ccttgaccat
    47221 tgtcaaaaat gtccactcgt atcgttcata aaacgtggta tgatctgtaa ttaagtaaag
    47281 tttttcgtac ccaagttgat gcatttcttt tctgatttcc gctaatatcc gcttggctat
    47341 cccctgctta cgcttttccg gttcaacaaa cagggcacaa agatttggtg tcagatcagg
    47401 gcggttatgg aagtcgtttt caatgacgcc cgctcctccg acgatggttt ctttttcatc
    47461 taagaccaca taccattgag gaatactttc cttattagcg attccagtac gaatactttc
    47521 ctcatacaca acagcaggaa ctccccattt tgatgaaaac caattcgcgc tttgttttac
    47581 taattctggg tgttctctta atttgatcca gttatatgta accaatgttt aaacctccga
    47641 ctaaaataaa acgtttacaa aaattataca agtctttatg aaaattagca atgacaacaa
    47701 tagtgatgta aaaaaaaaac ggaaaacaat cacttgttct ccgttttttg attaatcaag
    47761 catctaaagc ttcttttctt ctttcttgga tcgtagctac cttgacgaac ggtagataga
    47821 tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc acctgcaata ctttttgttg
    47881 ccaatacacc gccaataatt ggtggagtta cccatggagg cataacggta gctgctggca
    47941 ctaaacctgt agaagtcgca aagtaagcaa cactaactaa gaccattggt gtcaaaataa
    48001 atggaatgaa catgatcggg ttcaacacga taggtaaccc aaacatcgtt ggttcattaa
    48061 tattgaagac acctggcgca atcgacaagt ttgttacaac catatatggt ttgtttttac
    48121 gtcctactaa ataaatagct aacagtaaac ctaaagttgc acctgttcct cctaagttga
    48181 caaagctgtc aaagaaaggt ttgttcacga taaacggtgc gctatgccca gctgcgattg
    48241 cattaacgtt tgcttcaatg gctggcgcat tgattgtttg catcaaagga tcgaccatat
    48301 tcgctccgtg taaaccaaag aaccataaga atggtgtgat aaatgcaagc agtaaggcag
    48361 atggataaga gtttgcaagt cccataaatg gttcttggac cactgtatag aaagaaccta
    48421 cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag
    48481 tgatcattgc tggtagtaat gaagcaaatg cttttgcaac tgccggtggc acaccatcag
    48541 gcattttgat gatcaatttt tcatttccgc ttaaacgtac aaagatttct gttgaaataa
    48601 tcccaacaat aatagcaata aacaaaccgt ttgagctcat acctaatgct ccgcctaaag
    48661 cgaagaaaga gcctaaagaa atcacacctg cggaaacacc gtcttttcgg tagccgctag
    48721 ctaaatgata agcaagcaag aaagcaatca agacagaaag aatggcaaat gtaccgttcc
    48781 aagcagcagc cccgaatcct ttccatgatt caccaccaaa aattgagttc atcaattctt
    48841 ggaacccggg aatcggtaag ttattgatca ttactgctaa tgctcctaag atcattaacg
    48901 gcatactcac cataaatgag tcccgaatcg caactaaatg ccgttgtgca ccaatttttg
    48961 atgctacagg tataaaatgt ttctccataa atgcaacaaa tttatccata aatcttccct
    49021 cttttcttta ctgtagatta cgcgttcaat cgacgataca gatcaatgat ctcttttgct
    49081 aaatctgtaa aagctataga tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg
    49141 acttcaacgt gatttccttg tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct
    49201 tggactaaag attcctctgc atctttgatt ttttcatctg ctaaagtgaa atcacctttt
    49261 ttcgcagcag caatcgcttc cattgcatcg ctttttgcat ttccgccaaa catgatcaga
    49321 cccatgatgg cttctagatt tttttgatct tccatttatt gtatcctccg ataattttta
    49381 ttatttgttt attagttttt ctgcttggtc gagtactttt tcaccattca tcatgccata
    49441 gtcagccata ttgattacat ctaaaggaat gcctttaggt gctagctttt cagcaaattg
    49501 attttgcata aagcgtactt gcggtcctaa aagtaaaacg tctacatttt tagattctag
    49561 ttgattatcc gcatctgaag cagatacagc aaagatgtct gcttccattc cttttgcttc
    49621 tgctgccttt tgcatttttg tgactaggag acttgtactc attccagctg aacatacaag
    49681 catgattgtt ttcttttcca ttttaaacac gttcctttct atacttacac ttgtatataa
    49741 gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg atatcatcac atttaaggtc
    49801 tataccaagt tgcatacaca ctaaaaaaga aagcgcacac taaacacaca aattaatttt
    49861 tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact
    49921 tttttcaaat aaacggtatt caaaagcttg catctgcggt aataagtcca accaagttaa
    49981 aaacataaga tgttgttttt actttgctat tcttattact tgatgcagga cagctttttc
    50041 acaacctctc aataaacggt gttcaatcag ctgaccctgg gtattagctc aaaacgctgg
    50101 attcacaacc tcttttattt caaataacca gtaaatacaa tgaacatcta caaaaaactt
    50161 ctatttattt atagatttcg tcaggcaaat ccaattctct cttatcaatc aaagtctgat
    50221 accacgaagc agattttttc aatgttctgc ttcgttcatc gttcaattcg attcgaacta
    50281 atccatatcg attcttaaat gcattcattg gtgacacaca gtcagtgaat gcccataaca
    50341 tatagccttc gcaatttgcg ccttcttctg ttgctttgat caagtaatgc aaatgctgtc
    50401 cgataaattc gatacggtaa tcatcttgca cgattccttt ctcatccata tactgttctt
    50461 catattctct tcccataccg ttttctgtta tcagccaagg gatattatga taattttctt
    50521 ttagatacat ggctatatca tacatgattt tcggatagat ttcccatccc ctggaattat
    50581 tcattttctt cccaggtaaa tcaaagactt cataatatcg ttcaggatgg aaaggtgtct
    50641 cttcattcca ttgataacga ggggcgcgaa cgcgtttagg ataatattga ttgattccaa
    50701 ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa
    50761 tcgattgttt ttgacacaac tccaggaact cttctgtgta gtatcctttt accatcggat
    50821 caaaaaatac tcgattataa aacaaatcat acatataagc agctttttgg tcttctggag
    50881 aattggatcg agcatagacc atttcaggat ttaaaataca gccaatcctt ccaccataat
    50941 ttccgctgcg gtaaatctct actgctttag ccgttgctaa aactttatga taattccaca
    51001 tcatccactt ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg
    51061 tttggggaac gattggttcg ttgaatgtaa accagttttt taccctatca ccgtaacgat
    51121 caaaggcaat ttgagcatac ttagcataaa aatcaactac ttttcttgaa gaccagccat
    51181 catatttttc caaaagaaaa gctgggactt cataatgttc caaacaaatc atcggttcta
    51241 cgtctgcttc gatcaaagag tcgatcattt cacttatatg ctgagcatat tcttcatcta
    51301 ctatacagtt ctcataatca gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg
    51361 tcagatggat ggatttcatc agatccacat cctgctggta ttgttccata aaattagttg
    51421 ctacggctgg gccataccca ttatgccaaa caaaatgttc atttttatac cagctatcta
    51481 taaacgaatc ctgtccttct ttttttcctt tccaaccttc cgtttgccaa gcagaagatg
    51541 cagcaccaag aataaaattt tttggaatag taattttcat gatttcctcc tgttttttag
    51601 atcccatcat tttccacacg atttgctact tttacaaatg gcagatagat caaaatagaa
    51661 acaataatac aaacaagttg cgtaataact gcaccgatat ttcctgctgt tgccagataa
    51721 gcattgacga tcggcggcat tggccaaggg accattacaa ctgctttcgc cgcaaatcct
    51781 aaagcggttg caaaataacc aatcgttcca gtgataagag gcgttaagat aaacggaata
    51841 gctaaaattg gattcagcat gatcggcata ccaaaaataa ctggttcatt gatgttgaag
    51901 atcccaggag caagggataa cttggtgatt tgtttcatat cttcccgttt tccaactaac
    51961 aatactgcaa tcagtaaacc gatcgtgact ccagacccgc ccatactcat atacatatcc
    52021 caaaatggca tagtaataat gttcggtact tcttttccag cttcaaaagc ttccgtattt
    52081 acagcaattg ccgctaaaag caacggttcc cgtatcggct tcaccatttg atttccatgg
    52141 ataccaatca cccaaaagac ttgcgcaata aacatcaata gcaaaatacc cggtaaccct
    52201 tgtacgacac cttctaatgg gcgttgtacg acattgtaaa tgatatcata aaggtacatt
    52261 cctgtaagtt ctttgatgat aaatcccgca gccgcgatac ccgatacagt cagaatcgtc
    52321 gggaataaag cagaaaaact agcagaaaca ttcgttggca cggtatcagg cattctcagc
    52381 tgtagtttct tctgtttcct caaccagcaa taaagttcaa tagaaaggat ggagaccaac
    52441 attcccaaaa ataacccttt cgtatctgta tattgtttag ccaatacgtt ttctactata
    52501 acagtctgat tttcattcac taattcataa acagttggat taacagataa ataagacatc
    52561 actgcgatga taccagggaa tacaccatcc actttattca gcttggctaa ttcgatccct
    52621 atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga catagctgat ggcagatgcg
    52681 ataggcttta attcttctaa aaaacgcaag aaacttatct gtgctaagcc attttccgag
    52741 tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg taataattac cggaatcaat
    52801 gcagcaaatg cgttttttat caccatgata taccttaaat tattgattct cgttgccacg
    52861 ttccctaaaa cattggctaa tttatccata aaactcattc cagatcccct cattcctgct
    52921 tcttctcctt gacgtcatct ctttttgtct attagtcagc gcttacattt cgatgaacag
    52981 aaactattta ctcctctttt ttaccaaata taaaacaaaa attcatctga tgtatttatc
    53041 ataaaataca tcagatgaac tgtcaattag tttctttttc tcctattatt ttctgtaatt
    53101 ctctccgatt tattgccata tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc
    53161 tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc agatgctgga
    53221 tatcataatt tttattaaac aaagcaattg attcattaat gattgggata atatgcagca
    53281 aggctacgtt tccgctagct tctgcaatca tcgtatgaaa atcgatgtct aattgaacat
    53341 gttcttgtgt gccttctcga aaagaagttt caatcttttg cgttaatttt ttcatttgga
    53401 cgatttgatt cttcgttgca tattccgctg ccaaacctgc catcttaggc tccaataaat
    53461 atctcagttc aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga
    53521 gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt ccccgctcct tgccgaactt
    53581 caaggatatt cctggaagct agagcccgca cagcttctcg caccgtgctt cgccccacct
    53641 ctaaataatc ggacaattca tattcattcg gcagtttatc tcctacttgc aactgcattt
    53701 cctttaaata tctaaggatc ttgtcagtag ttacttctac tagggtttta ttttcttcca
    53761 aaccacatac ctccttttca tctgatgtat tttttcgtaa ttattataac acgtttgaat
    53821 catctgatga ttttttgtat acaaaattga ttaatccgtt catagacata caaacgaaga
    53881 agatatttac cataaaaaag actaggataa aattcactcc tagccttcga tcatttttat
    53941 tttgaacgac aaaaaatatg atgttcaata acagacgatc ttcattcgat tcttgatgtc
    54001 atcttttgtt ttttttaata tatggataaa aagtttgatt acatcgtttc tattctagaa
    54061 tttggtcgat tctcaattct tcttctggtg agaaatctaa attatccatc attcgaacac
    54121 tttcttgtaa ctgacttaac cgacttgctc cgaccagtac acttgtaaca gatttttgtc
    54181 gcaaattcca tgcaagggcc atttgcgcta atgattgtcc tcgtgatacg gcgattgttt
    54241 gcagtgcttt gattttttcc agagttgatc caacttgttc ttctgaaagg aatggtattt
    54301 ccttacgatg tgctctagaa tcttcaggaa taccatgtag ataacgattt gtcagcaacc
    54361 cttgagcaag cggactgaac gtgattgccc ctagcccttc ttcttctaat actggagata
    54421 gatcgtcttc tagtagtgca cgagaaaaca tattgtagcg catctgatga ataatgaacg
    54481 gtgcttcctt tctttttaaa atttcagtca tttttttgta tcttctccat tatagttgga
    54541 tataccaata tataatgctt ttccttgtct tacaagttgc atcaacgctt ctgctgtttc
    54601 ttccaaaggt gtatcagggt ctgggcgatg atgatagaag atgtcaacat aatctagccc
    54661 cattctttgg aaactttggt cacaacttga tatgatggat ttacgagagc cccattcccc
    54721 atatggccct tcccacatat gataacctgc tttgctagaa ataatcagtt catcacggta
    54781 ggcttgaaaa tcttctctaa aaatacgccc aaagtttttc tctgcagctc cagcagggcc
    54841 tccataatta ttcgcaaggt cgaaatgggt aattcccatg tcgaatgcgc ctcttaaaat
    54901 ctctctttga ttatggatag gatcataatc accaaaattt tgccataatc cgagaggaga
    54961 gataaaatcg gtaattttaa acctgaatga ccggatttac gatattccat tttttcataa
    55021 cgtgcactat ctgcaatata caatttttct cctcctcatc aaatcttttt aaaacgcttt
    55081 cttgttttct atttttagtt tactacattt taaagaaaag gagttaatca aaatgatttt
    55141 ttcgtatatt tttttgccat ttgtttttta ctcgttgata gatcagaaat tgattacgtg
    55201 cttgctggat ttttttatag cgtttcggtt gttttttgta aaattgctga tgatagtttt
    55261 ctgctggcca aaacgtaccg gctggttcga tttttgtgac gattggttgg tggaaagttc
    55321 ctgaactgtc tagtttctgt tttgattgtt ccgctaattc tttttgtcgt tcgtcttgat
    55381 aaaaaatcac tggacgatat tgcttccctc tatcttgaaa ttgtccaaat gtatctgtgg
    55441 gatcagtaat ttgccaatag attgtcagca actcgctata acttatttct cttgtgtcat
    55501 aaatgatctc cacagcttcc acatgacctg tgtaaccacc acttacttga tcataattcg
    55561 gtttttcaac atgtccgccc gtgtatcctg acaaaacaga taggatccct tttttcgatt
    55621 caaacggctc gaccatacac cagaaacatc cccctgcaaa tactgcacgt tcttgatagt
    55681 cagcatcttg catggcatgc ttggaaaagt catatttttg ctccttatca aatccttccg
    55741 taatcaataa ataaaagtcc attacatcag gcgtcagatt cattcggata gctaatggac
    55801 gtagtgttac ttctagtttt gataactgtt ctttaacgga tatattttct cttgtgtcat
    55861 tctttgtctg aatcaatact tttcgttccc aatctcgtgt attcggattc aaaatcagat
    55921 tataaagtgt ttgaatgaat atttgttcct catccataga ttctcctcct ttccgaagaa
    55981 gacaagctag tgactttagc tgtaatttat cataaagtga ggaaataatc gattgaatcg
    56041 tatttattca aagaagataa gcagcttcag atgatataag atgatcttta tcttaaaaag
    56101 catactctat ggtttggttt aactaaaaaa cgacctctga aaaaagggat gttctccaat
    56161 tctcagaagt cgagtaaaga tgattctttt ttatgagcga taataaactt catccaaaaa
    56221 cagaccttgt gcagggacgg tttctcctgc atgttgccgc actttgcttt caaatatttc
    56281 atcgattgtt tctatcggca tggcacctgt gccgatttct agtaatgtcc ccatgataat
    56341 ccgcaccatt ttatataaga atccgtctcc gacaaaagta aaatgaagca tttctccctt
    56401 tcgttcgata gataattcat caatcgtacg cactgtggat ttttttgatt ttttcaatgc
    56461 agaaaaacca atgaaatcat gtgtacccac taatttttcg caagctgcat tcagcttgtc
    56521 catatccaac tcttgaggga aataaaagct gtggtttcgc tcaaatgctg aaggaatcac
    56581 gttgttccaa acataatagc tgtatttttt gcccaccgca ttatatcttg aatggaagcg
    56641 ctcaggcatt tcttcgattc ttttgatgac aatatcccgc ggaaggtaac gattcataaa
    56701 gtcgatcatt tctgccagtc caatttctgt gtttgtttta aaatttgcta cttgtcctcg
    56761 cgcatgggtg ccagcatctg ttcttcctga tccaataatt tctatctctt catttgtcat
    56821 ctgatgcaat actttttcga tttttccttg aatggtttta tcgtcatctc caagtctttg
    56881 ccatcctgaa tatctttttc cgtcatattc gattgtcaac ttgatgtttc tcatatttta
    56941 gtggctcctc tgtcttccat ttggtcttct ctctaccttt ttaagattgt agcacaaaat
    57001 atagctcctt gcttctctaa gacagaagct atacaagaaa actgtggaaa tatatatttc
    57061 agaaaaacaa aaaacagcca ccggatgaag tatccgctga ctgttcaatt tgacggctca
    57121 agttttactg tttgagatgc aagatactca accaggcaat gggcacataa ttaaagaaaa
    57181 aactgattct ttggcaaaaa cctattttat tcagtaatgg ccaagaattt atagaaggaa
    57241 ttcttgccag tccataaatc gccgcaaaaa gcaaactgat cacagacagt ccaaggtata
    57301 agtggcttac gtccatttgc ccattttttc gatagatcaa gaaaagaaga actggaaaca
    57361 ataaaaaacc actgtaacct aaacctgatc cgatattatg gacccatgtc gagacattcc
    57421 acgaagattc atgggtattg atactgaaca aacctgtaaa gatacaatca cctataccat
    57481 agaacgcgat ggctgctgta actagggtag ctaacacacg agatgtactt acaaatgcag
    57541 catacaatgc aggcaatgac aggatgaaaa agatccctga aaccacagac caaataagaa
    57601 acgcttttct tacaggactg gaaacctctc caaatacact gatcacgctc ttcatttgat
    57661 ctaattcagg ataatacaat cctaatacgt acggagtcaa aaaatcactc agcacgccta
    57721 ataataaaaa gaaaaaacca aatttacgta aaaaatccat gatacgcctc caataacaat
    57781 tacctatgct tttaattgtt attataaacg actttatctt gatttaacag aaacctctat
    57841 ttacacgaac atctatataa ctcattaaaa actcgaatca ttttaaaaat actaaatatc
    57901 taacgttcaa cgttcactta atccatctaa acaatctttc aatttgattt tttccccact
    57961 tcttttacgc gcgcctagct ctcctattgc agtaaacaac agatcacttg acgaatttaa
    58021 ggcagtttcc attgaatcct ggataacacc gataataaat ccaataccaa cgatttgcat
    58081 cgcgacatca ttagagatac caaacaaact acatgctaat ggaatcaata acagtgaacc
    58141 gcccgcaaca cctgaagctc cacaagcagc taaagcagac aaaatacata aaaggacttt
    58201 caaaacaaat ggtgcttcaa tccctaaaga atgtacagca gtcaacgtca tcaaggaaat
    58261 ggtaatcgcg gcaccgccca tattgatcgt agcacctaac gggatcgata tggaataaga
    58321 ctcttttgtc aatttcaatc tttcagccaa catcatgttt atcgggatat ttgcggcaga
    58381 acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa caaaagaaaa tcaatggata
    58441 gggattttct ttaataaaca aaaacgtgag gaatgggtac acaactaatg cgacaaacag
    58501 catcgtaccg atcaaaacaa gtaaaagctg cagatactct gccaatccag cgattccagt
    58561 cgtagcaatt gattgataaa ccaatcctaa aatccctatc ggagcaaatg caataatcac
    58621 ttgtacgact tgtgacaacg ctatagatat ctgatccacc aactcttttg tcggttctga
    58681 acgtgctcga aaagctagtc ccaacaatac agcccagaat aaaacgccaa ggtaattcgc
    58741 atcaataaga gattgaacag gattttgtgt gacattcgtc aacatagtag ataaaacact
    58801 ttccaaactt tgcggtgctt tttcagatac tgtttcttgt aaagtaatag gaacagtaaa
    58861 aattgaactt acaatgactg ctacaagtgc agaaagcaat gttcccacta aatataagat
    58921 caaaataggc ttcacgaatg tcttattccc aattttatgc ttggcaattg acgccatcgt
    58981 caagaaaaaa accagaatag gtgcgattgc cttcaagcaa ctgacaaaca acgtccccaa
    59041 tattgaaata aatgtccacg tggggaaaaa gataccaaac aatattccga ctaaaatacc
    59101 aacaacaata cgttgaacta acgacatatc caaaaacttc tctaacaaat gaccaccctt
    59161 tcacaatttt aacaatcatt gtatcatttt atctaaagaa actaaaggtg caattttcta
    59221 aaaattctaa taatacaatt ggcaattcat gctcttgcaa cattcaattt tagtattgga
    59281 gggtttattc aatctctaaa gaagtcgaga gaaaactatt tatcgttatt attatagtta
    59341 tccaagtaaa acgtaactaa gatgaaggac gaaactagaa atttcatcct tcatcttaat
    59401 tcttagacat agcaatcaat taatattttc cactgagtct cttctttctc tcactctcac
    59461 tggtttactt acccataaac gatttgatta aagccatttt tcaattcatt attcgctgtc
    59521 tcgttaaagt aaagataatt atcgggattc ccctgatttt gttgtgtcac caatactttt
    59581 ggctgatttt gctgaaaccc gaatagatag acgtgctttt tcagatatgg ttctgtctct
    59641 gcatcggaat agacggctgt gatttgatag tctgcttgac ctgtcccact ttctgaccat
    59701 tctgcagaaa caggcactcc accaatagcc atcttccact ccccgttcaa aataacttga
    59761 gggactttaa gaccatataa gtccacactc atttgattcg tatagctttt gtattgttgt
    59821 cccattgttt ggccccatgt agccataaat gcctctaatt gtgcggattt gtctgcattc
    59881 cacagtgttt gttcgacttg ttcgctgctt tctgaagatg aagcctctac agaagaactt
    59941 gaagtagatg tttgagttgt ttcagtcgtt ttggaagact ctgacgaagt agaggtatct
    60001 gctgtttccg tacttgctgc agttggcttg ctctctgtgc tagttgttgt agtagttgca
    60061 attgttgctt tcgctgtttc ttttgttgat tcttttttcg attcattccc gttggtacaa
    60121 ccagctaaca aaaaagctag accaattgtt actaaaatcc tttttttaaa ttaaaaaact
    60181 cctttcgatt actcccactt ctttattcta tcaaataagc aatgtgattt tattgatttt
    60241 ttgtattttc tcatccatct tttaaacctt ttaaaaacta agaagcaccc gctgatgttg
    60301 tctgtaatac atcagcgggt gcttctttta ttttaatctt cgcgattttc caatttacct
    60361 cacgctttat atttgtattt gaagaaacat agacttttag tcatcatcgt cttcctcatc
    60421 caaatcttgt cggacagttt ttccttggag tctttgtact tggtagatga tttctttatt
    60481 gaaacttaaa agttcaacaa gcaattgttt caagccatat ttttcttctt tttcagctaa
    60541 tttgattgcc cgtgtgataa agacattttg gaaatcaaaa tcttttgcaa agttttccaa
    60601 cattgttgag gctggtaaat atttgttttg accatgttcg taaatttttc cataacgcat
    60661 gacttcttct aaagtagacg gaacgatttc attttcaatc actaactcat gagagatttt
    60721 attgaaccat ctcgcttctt gatcagcata ttctgaaaaa agggtactta attcttgcga
    60781 agatgtaccc aaaacataca attgtgcttg gatcaatttc atttcttcta atttaaaatt
    60841 tgccaataca tgccccacca tggcgccagc agttggttta tgatgatcga tatctgcttg
    60901 ttttgtttct tgagcaaatt tttcttccac tatattcact gatttacgcc tcttttactt
    60961 ttgttgattg gacttcataa cctaattttt ccacaactgc agctaattgt tcagggtgga
    61021 cttcatctgg atcaaagttt gctttgattt tgctcgcatt aaacaaaact ttcgtattgc
    61081 ttaccccagg ctgttgagaa accgcatgtt ctattttttg catacagctt ggacaagcta
    61141 gtgtatctaa ttgcataatc atttttttca tcgtagttct tccttccttt ttcttgatag
    61201 tcttattcta gattacagct tccataaaaa aattgattct tatcaagttt tacgattttt
    61261 cttctttgct ttcgtgtcca aaatacaata aacgcatcgc gttaaagata acgatcaaga
    61321 tactagcttc gtgaacgaac atcccactag ccatgtaaat aaacccggca aataagccga
    61381 tgagtaaaaa taagacgaca ccaatggcaa tcactacatt ttcacgggta ttcaaaacag
    61441 ttttcttagc caaacggtaa gcatgaacca acgatccaaa actagattgc atcaagacaa
    61501 cgtcactcgt ttcgatagcg acatccgttc ctgatcccat agcaatcccg atatcagccg
    61561 cagccaaaga gggagaatca ttgattccat ctccaatgaa agcaacgtgc aatccttgtt
    61621 cttggtattt tttcacaaat tgaacctttt catctggcaa taattcagca tggacttcat
    61681 caatgcctaa catgtcagct acataatcag cagtcatctg attatcacct gtcagcatca
    61741 ctaaatgttt tgctcctgct tgtttcaaca tagctaattg ttcagcgact tctggtcgaa
    61801 tcacatctga cacaccaatg atttgagtga tttttttgtc gattgctaca ataatgactg
    61861 agctgcctct ttgttgtaat tgatgtaaat cttgtacttg ctttggtgtg agagaaagtt
    61921 tgtgagcggc aatcaatttg gtgttgcccg cacaaactgt atgaccatca atctcagctg
    61981 tgattccttg tcccttgatc gtctgattat ctttgactgc taattgttga aaatcaatgt
    62041 ctttctcctg agcataatgg atcacggcgc gccccagagg atgatcggat aacgtttcta
    62101 atcgagccac taaagctaag ctgtcccctt catcctctgt atagttcttg atagtggata
    62161 cagcagtagt tcctttggtc aatgtaccgg ttttatcaaa gacaaaagta tcgattttgg
    62221 aaaaagtatc cattgcttca ccgcctttga ctaagatgcc ccgtttcgct ccattaccga
    62281 ttcctgctac attcgaaaca ggcgcaccaa tgactaaagc gccaggacaa ccaagaacca
    62341 aaacagtgat ggctaaacga aaatcacgac tgatcagacc aacaagcaac gcaataagta
    62401 aaacggttgg agtataatac tgagaaaatt gatcaatgaa tttttccgca tgtgatttgg
    62461 tatcttgtgc ttcttcgact aattctataa ttttggcaaa ggtcgtatca tctccgacct
    62521 tagttgcttc gatcgtcacg tagccatctg ctaaaatcgt tccagaaaat acgttatctc
    62581 cactgatttt gacgacttgt cgtgattcac ccgtcactgc ggcttcatcc gcataccctt
    62641 tcccgtcaat gactcgtccg tccacaggaa tagctccacc agtttttact aagacgatat
    62701 cgccttcttc cacgtcatcg atttccactt cttcggtcgt tccatcttct tggacaacgc
    62761 tggcagtcga tggggccatc tcggtcaaag ccttgattga ttcccgcgtt ttggtcaatg
    62821 ttttttgttc cagataacta ccaaacaaaa acaagaatgt aacgatagct gactccttgt
    62881 attctccgat cataaaggca ccaagcacag caatagtaac gagtaaatca attgagataa
    62941 ctttgacttt tactgcctga tacgcatgga ttataatagg tatgaccgca ataatagaag
    63001 ctatggctag agcaatgtta tagaacaaga caaatccagt aaatttcccg ataaaaccaa
    63061 aaataatcaa cacagctgat aaggcggtga tatgatttcg atgctcttgg atccactttt
    63121 gtaatttcat ctttctccct cttttccttt tcattcttac gagactaagt atagatactt
    63181 atccatcata aaaaattgac cactatcaag tttattcaaa aaggcggttt tcttttggta
    63241 tactgaattc ctacagtcat taaacgatca tatagaaaaa ggaagcgccg aaacgcttcc
    63301 ttaggtagtt ctattttttt attcaaagat aagatgcttt gtcatcttac gactgaagaa
    63361 caacaatcaa aaaagggcag cagccactca ttcacaagaa atatcgtttt ttgatatggg
    63421 agttgaagca gtggacaaat atctctactt attagctggg aataagatcc aaaaaagctt
    63481 aatggatttt atccaagaat tggaatgtac tttccacaaa aaattcactc attccattct
    63541 tttaaaactt ttgattcata cagcgtgtct aattgagcgt acattgataa acggtcatga
    63601 acttaaaatt atatcagaag atgacacaag accttctcat gaaacaattt ttcatgtaaa
    63661 aaaggccttc aaaaatattg aaacagaatt tgggatcact gtttcttatg atgaatgttt
    63721 ctttatctat gatattatcg catctaaata atcttttttt atgtaaacga gtattttggg
    63781 agcgtcaata attttgtgta aataaattgt cctcctgcaa aataattagt tactcagtaa
    63841 acattgaaac taatgtatcg gttac
//
LOCUS       DASHFF010000008        61163 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.008, whole genome shotgun sequence.
ACCESSION   DASHFF010000008 DASHFF010000000
VERSION     DASHFF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.008"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            432..3239
                     /locus_tag="U2I75_000595"
     CDS             432..3239
                     /locus_tag="U2I75_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1695752.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3430..3921
                     /locus_tag="U2I75_000596"
     CDS             3430..3921
                     /locus_tag="U2I75_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1695753.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            4024..5013
                     /gene="manX"
                     /locus_tag="U2I75_000597"
     CDS             4024..5013
                     /gene="manX"
                     /locus_tag="U2I75_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN1695754.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            5046..5849
                     /locus_tag="U2I75_000598"
     CDS             5046..5849
                     /locus_tag="U2I75_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN1695755.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5870..6784
                     /locus_tag="U2I75_000599"
     CDS             5870..6784
                     /locus_tag="U2I75_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN1695756.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6867..7241
                     /locus_tag="U2I75_000600"
     CDS             6867..7241
                     /locus_tag="U2I75_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN1695757.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7380..7625
                     /locus_tag="U2I75_000601"
     CDS             7380..7625
                     /locus_tag="U2I75_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1695758.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7797..8861
                     /locus_tag="U2I75_000602"
     CDS             7797..8861
                     /locus_tag="U2I75_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1695759.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9107..9532
                     /locus_tag="U2I75_000603"
     CDS             9107..9532
                     /locus_tag="U2I75_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1695760.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9525..9974
                     /gene="nusB"
                     /locus_tag="U2I75_000604"
     CDS             9525..9974
                     /gene="nusB"
                     /locus_tag="U2I75_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN1695761.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10071..10925
                     /locus_tag="U2I75_000605"
     CDS             10071..10925
                     /locus_tag="U2I75_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN1695762.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10926..12263
                     /locus_tag="U2I75_000606"
     CDS             10926..12263
                     /locus_tag="U2I75_000606"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN1695763.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12268..12492
                     /locus_tag="U2I75_000607"
     CDS             12268..12492
                     /locus_tag="U2I75_000607"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN1695764.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12494..13384
                     /locus_tag="U2I75_000608"
     CDS             12494..13384
                     /locus_tag="U2I75_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1695765.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13393..14211
                     /locus_tag="U2I75_000609"
     CDS             13393..14211
                     /locus_tag="U2I75_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN1695766.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14318..14767
                     /locus_tag="U2I75_000610"
     CDS             14318..14767
                     /locus_tag="U2I75_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1695767.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14785..16467
                     /gene="recN"
                     /locus_tag="U2I75_000611"
     CDS             14785..16467
                     /gene="recN"
                     /locus_tag="U2I75_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN1695768.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16522..16638)
                     /locus_tag="U2I75_000612"
     CDS             complement(16522..16638)
                     /locus_tag="U2I75_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN1695769.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16711..17667)
                     /locus_tag="U2I75_000613"
     CDS             complement(16711..17667)
                     /locus_tag="U2I75_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1695770.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17838..18206
                     /locus_tag="U2I75_000614"
     CDS             17838..18206
                     /locus_tag="U2I75_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN1695771.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18377..18808
                     /gene="mraZ"
                     /locus_tag="U2I75_000615"
     CDS             18377..18808
                     /gene="mraZ"
                     /locus_tag="U2I75_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN1695772.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18829..19788
                     /gene="rsmH"
                     /locus_tag="U2I75_000616"
     CDS             18829..19788
                     /gene="rsmH"
                     /locus_tag="U2I75_000616"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN1695773.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19793..20200
                     /gene="ftsL"
                     /locus_tag="U2I75_000617"
     CDS             19793..20200
                     /gene="ftsL"
                     /locus_tag="U2I75_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN1695774.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRIRKVK"
     gene            20201..22393
                     /locus_tag="U2I75_000618"
     CDS             20201..22393
                     /locus_tag="U2I75_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1695775.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22419..23381
                     /locus_tag="U2I75_000619"
     CDS             22419..23381
                     /locus_tag="U2I75_000619"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN1695776.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23395..24756
                     /locus_tag="U2I75_000620"
     CDS             23395..24756
                     /locus_tag="U2I75_000620"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN1695777.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24782..25870
                     /gene="murG"
                     /locus_tag="U2I75_000621"
     CDS             24782..25870
                     /gene="murG"
                     /locus_tag="U2I75_000621"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN1695778.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25885..27105
                     /locus_tag="U2I75_000622"
     CDS             25885..27105
                     /locus_tag="U2I75_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN1695779.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27261..28586
                     /gene="ftsA"
                     /locus_tag="U2I75_000623"
     CDS             27261..28586
                     /gene="ftsA"
                     /locus_tag="U2I75_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN1695780.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28609..29850
                     /gene="ftsZ"
                     /locus_tag="U2I75_000624"
     CDS             28609..29850
                     /gene="ftsZ"
                     /locus_tag="U2I75_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN1695781.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29867..30544
                     /locus_tag="U2I75_000625"
     CDS             29867..30544
                     /locus_tag="U2I75_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN1695782.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30558..31157
                     /locus_tag="U2I75_000626"
     CDS             30558..31157
                     /locus_tag="U2I75_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN1695783.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31172..31459
                     /locus_tag="U2I75_000627"
     CDS             31172..31459
                     /locus_tag="U2I75_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN1695784.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31508..32290
                     /locus_tag="U2I75_000628"
     CDS             31508..32290
                     /locus_tag="U2I75_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN1695785.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32311..33018
                     /locus_tag="U2I75_000629"
     CDS             32311..33018
                     /locus_tag="U2I75_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN1695786.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33280..36078
                     /gene="ileS"
                     /locus_tag="U2I75_000630"
     CDS             33280..36078
                     /gene="ileS"
                     /locus_tag="U2I75_000630"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN1695787.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36127..37653)
                     /locus_tag="U2I75_000631"
     CDS             complement(36127..37653)
                     /locus_tag="U2I75_000631"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN1695788.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37668..38315)
                     /locus_tag="U2I75_000632"
     CDS             complement(37668..38315)
                     /locus_tag="U2I75_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN1695789.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38499..38828)
                     /locus_tag="U2I75_000633"
     CDS             complement(38499..38828)
                     /locus_tag="U2I75_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN1695790.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            39005..39733
                     /gene="sfsA"
                     /locus_tag="U2I75_000634"
     CDS             39005..39733
                     /gene="sfsA"
                     /locus_tag="U2I75_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN1695791.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39749..40762
                     /locus_tag="U2I75_000635"
     CDS             39749..40762
                     /locus_tag="U2I75_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1695792.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40762..42039
                     /locus_tag="U2I75_000636"
     CDS             40762..42039
                     /locus_tag="U2I75_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1695793.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42102..44804
                     /locus_tag="U2I75_000637"
     CDS             42102..44804
                     /locus_tag="U2I75_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1695794.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44956..45273
                     /locus_tag="U2I75_000638"
     CDS             44956..45273
                     /locus_tag="U2I75_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1695795.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45303..45623
                     /locus_tag="U2I75_000639"
     CDS             45303..45623
                     /locus_tag="U2I75_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1695796.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45731..47191
                     /locus_tag="U2I75_000640"
     CDS             45731..47191
                     /locus_tag="U2I75_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1695797.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47259..47480
                     /locus_tag="U2I75_000641"
     CDS             47259..47480
                     /locus_tag="U2I75_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695798.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47511..47693
                     /locus_tag="U2I75_000642"
     CDS             47511..47693
                     /locus_tag="U2I75_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN1695799.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47693..48106
                     /locus_tag="U2I75_000643"
     CDS             47693..48106
                     /locus_tag="U2I75_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN1695800.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48229..49410
                     /locus_tag="U2I75_000644"
     CDS             48229..49410
                     /locus_tag="U2I75_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1695801.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49481..49645)
                     /locus_tag="U2I75_000645"
     CDS             complement(49481..49645)
                     /locus_tag="U2I75_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695802.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49799..49872
                     /locus_tag="U2I75_000646"
     tRNA            49799..49872
                     /locus_tag="U2I75_000646"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49833..49835,aa:Arg,seq:cct)
     gene            complement(49941..51080)
                     /locus_tag="U2I75_000647"
     CDS             complement(49941..51080)
                     /locus_tag="U2I75_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1695803.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51379..52014)
                     /locus_tag="U2I75_000648"
     CDS             complement(51379..52014)
                     /locus_tag="U2I75_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695804.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52127..52762)
                     /locus_tag="U2I75_000649"
     CDS             complement(52127..52762)
                     /locus_tag="U2I75_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEN1695805.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(52796..53257)
                     /locus_tag="U2I75_000650"
     CDS             complement(52796..53257)
                     /locus_tag="U2I75_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEN1695806.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(53387..53818)
                     /locus_tag="U2I75_000651"
     CDS             complement(53387..53818)
                     /locus_tag="U2I75_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1695807.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53836..54156)
                     /locus_tag="U2I75_000652"
     CDS             complement(53836..54156)
                     /locus_tag="U2I75_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1695808.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54455..55231
                     /locus_tag="U2I75_000653"
     CDS             54455..55231
                     /locus_tag="U2I75_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN1695809.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            55246..55449
                     /locus_tag="U2I75_000654"
     CDS             55246..55449
                     /locus_tag="U2I75_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1695810.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            55465..55803
                     /locus_tag="U2I75_000655"
     CDS             55465..55803
                     /locus_tag="U2I75_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEN1695811.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            55790..55969
                     /locus_tag="U2I75_000656"
     CDS             55790..55969
                     /locus_tag="U2I75_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695812.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(56012..56482)
                     /locus_tag="U2I75_000657"
     CDS             complement(56012..56482)
                     /locus_tag="U2I75_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695813.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            56569..57267
                     /locus_tag="U2I75_000658"
     CDS             56569..57267
                     /locus_tag="U2I75_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN1695814.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            57445..57786
                     /locus_tag="U2I75_000659"
     CDS             57445..57786
                     /locus_tag="U2I75_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695815.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            57779..58450
                     /locus_tag="U2I75_000660"
     CDS             57779..58450
                     /locus_tag="U2I75_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN1695816.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            58456..59142
                     /locus_tag="U2I75_000661"
     CDS             58456..59142
                     /locus_tag="U2I75_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695817.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            59145..59894
                     /locus_tag="U2I75_000662"
     CDS             59145..59894
                     /locus_tag="U2I75_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1695818.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            59906..60175
                     /locus_tag="U2I75_000663"
     CDS             59906..60175
                     /locus_tag="U2I75_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695819.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            60180..60344
                     /locus_tag="U2I75_000664"
     CDS             60180..60344
                     /locus_tag="U2I75_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695820.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            60337..60639
                     /locus_tag="U2I75_000665"
     CDS             60337..60639
                     /locus_tag="U2I75_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695821.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            60636..60797
                     /locus_tag="U2I75_000666"
     CDS             60636..60797
                     /locus_tag="U2I75_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1695822.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            60794..61099
                     /locus_tag="U2I75_000667"
     CDS             60794..61099
                     /locus_tag="U2I75_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695823.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT        20501 a        10231 c        12838 g        17593 t
ORIGIN      
        1 gaatcttatg attaaagagg ttgtgacaag acttttgtca cagccttttt tgtttataaa
       61 aataaaagtt gtagtggagg ctacgaagtt tactttgtct ttttacactt aatatttttt
      121 tgccaatttg gaatcaggat gttttgataa ctatcgtttt caattgatgg aagctatatt
      181 gtttgctatc agtgaaataa ttatatggta aacagtgttc tttttcttgt ttccattata
      241 aaaatcgtat cgggattttt cgttctttta aattgaacat ttctatctat ccgcgtgtta
      301 tcaacaaaac aaaatcaggt aaatagttga cgaaacagaa ataaatacat agtattatgt
      361 tttgaagcct aagtacaata gtctatagaa aaggagcgat accattggca aaacgcattg
      421 accgaattta tatgtatgtc aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc
      481 aaggagtaac aacacaggaa attgctgaag tgttagggat tcagcgaaca aatagcagta
      541 aagatctgaa tcagctagtg cgcgaaggaa aactgataaa gacagacgga cgaccagtac
      601 gttacatata tcaaacactc gttacgcacg taaagccagt tacaaaacat gtagtcagtt
      661 acaaagaaac gccggtctct gaagaaaaaa atacactcca aatagatact aaggatatct
      721 ttgcaaaaat cattggagct aacggcagca tgaaaaattc agtggaacag gcaaaagctg
      781 cgattttgta tccgccaaga ggattaaatt gtttgattac aggacctacc ggatcaggaa
      841 agacttattt tgcacacgcg atgtatcatt ttgcaaaagc gcatcatgta atagatgagg
      901 aaacggaact gattgttttc aattgtgctg attatgccaa taaccctgag cttttgatga
      961 gtcatttatt tgggtacgca aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga
     1021 ttatcgatca agcggatggc ggtatgctgt ttttggatga gattcaccgg ttgccgcctg
     1081 aaggtcaaga aatgatcttt tactttatgg atcatggaac ttacagtcgt cttggggaaa
     1141 caacgaaatc ccatgaagca aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt
     1201 cttctctatt agaaactttt gttcggcgta tcccaatcaa tatcaaactt ccggcttttg
     1261 aaaaacgtcc tgcaaatgag aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa
     1321 accgcacgca gcgaaagatt tctttgacag aagatgtagc aaaagcattg atcggcagtg
     1381 tgacctatgg aaatatagga caattgaagt caaatatcca gttagtatgt gcacgaggat
     1441 ttctaaatca tatgcaatca cccgaaatct cgatcacaat cgatgatttg acagaaggga
     1501 ttcgtagcgg attgattcaa ttagcaagca accgcagtgc aatggcagag ctttctaaaa
     1561 tattggagcc aaagataact gtagcaccaa atgatacgat catgaaaatc caatcggact
     1621 cctatgagtt accttataat ctatatgata tcatcggaga taaagcagct ttactgaagt
     1681 cggatggttt ggatcaagaa gcaatcaatc atttcatttc tacagatatc aacatccatc
     1741 tgaagtcttt ctataaagac catggttttt cttttaatgc agacagcaaa ttagcagaat
     1801 ttgtagatcc gaaaatcata gaagtgacca atcagattta tgtaatggtc aaaagatctt
     1861 taccatatga gtttcagcaa aactttattt atgctatgag cttgcatatc agttcttttt
     1921 tgaaaaaaat caacttagga gaagaacggc atacgaatga taacatccgt gagatggctt
     1981 ttgattttcc agatgaatat gctgttgcga aagaagtgcg tagttatatc gaaacgtatt
     2041 ttcaagtgga gattccagaa agtgaagact attacttgac cgttctactc gtctcgttac
     2101 gggcaaatca agcaagcggc agaatcggtg tagtcgttgc agctcacgga aacagcacag
     2161 caagcagtat ggtccaagtt gtccaacagt tgttagatgc agagaacgta cgagcagtag
     2221 acatgccttt ggatatggat ccgaaaacag cattggaccg aattgaacgc aatgtacaag
     2281 aagtagatga aggaagcggg gtcatactct tagtagatat ggggtccctt gcgtcgttta
     2341 attcgcaaat tcaaagagac actggaattc cagtacgtac tgttgatatg gtaacaactt
     2401 ctctagtttt ggaaactgta cgaaaggtct cagtattagg gacagatcta gatatgttgc
     2461 atgaatcatt gaaaaatttt cgaggttacg cagaaatatc ttctgtagaa tctgttcagg
     2521 aagcaaaacc tattcataag aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta
     2581 cagcacaaag aatcaaagaa ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat
     2641 tagaagttct agctttgtcg attgtggaag tgaaagaaga actgccagtt atccaaaaag
     2701 aatatcagat catagctact actgggatta ctgatttgaa tatccaagcc ccatttattc
     2761 ctttagaacg ttttattgat caaaatatcg aagtcatttt ggatcagttg ctgatggaat
     2821 cagaattgga ggaaactgaa ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg
     2881 ttgagtttat ctctgataac ttcatcttta ttaatggtag taagttaatc gatccaatgt
     2941 ggcaattttc tacacaaatc tctcagacga cgggaatcgg cgatgaagaa tatggattca
     3001 aaatcaacct agtgatgcac acagcaggaa tgatcgaacg tattattcgg aatgaaccat
     3061 tgactgtgga agaaaacgaa ttgacaaata caacgaatga tcctctttat agccaattgg
     3121 cagcctcagt tgttctgttg gaagatcaga taaaagtaaa agtgccgata gaagaaatgt
     3181 attatctgct acgtctagtc cataatcagt tagataaaaa agaatacact gtgccatgaa
     3241 tacacaaaaa cgatacactt ttatagtgta tcgtttttgt gtattggtta agaatccttt
     3301 aaattcaatt agtgtatcgt aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct
     3361 aggaggtggt tgttcacaca gaaatgatag gggtttctgt gaagtagtga ggaagaagga
     3421 gttttgcaga tggatattcg attggcacgt atagatgatc gtttaatcca cgggcaagtc
     3481 gcaacagttt ggtcgaagca aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc
     3541 gctcaagata aattgagaaa gtttctacta aaagaagctg caccgccggg gatcacagca
     3601 aatgtaataa ccgtgcagaa aatgattcaa atctatcata gtggattatt atcacgagca
     3661 aaagtgatgc ttttattcac caatccccaa gatgttgaag cggttgtccg ctgcggtgta
     3721 cagttgaagt cgttgaatat cggcggtatg agttttactg aaggcaaatc aatgattacc
     3781 aattttgttt cagtaaatca acaagatatt gattcctttc gttacttgaa cagtcaagaa
     3841 gtggagttag agattcgaaa agtacctgct gacaggaaaa tccttttgat ggaactacta
     3901 gcgaaaatca aaaaggtgta gcaagaatgg ttgttggaaa aggtcaaata attctgttgt
     3961 aatggttagc aattattaat atttttgaat ttgttacaac aaaagtaaat aggaggtata
     4021 aatatggtag ggattatcct agcaagtcat ggacaatttg ctgaaggaat cttacaatcc
     4081 ggttcaatga tctttggcga acaagaaaac gtcaaagccg tgattttgaa accaagtgaa
     4141 ggtccagatg acttgagagc caaattggaa gaagcggttg cttcttttga taatcaagac
     4201 gaagtgttat tcttagttga tttatggggc ggaacaccgt tcaatcaatc aaacacatta
     4261 ttcgaagagc acaaagataa atgggcaatc gtcagcggtt tgaatttacc aatgttgatt
     4321 gaagcctacg catcacgctt ttcaatggaa tctgctcatg aaattgcagc acacatcatt
     4381 gaaacagcaa aagacggtgt aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg
     4441 aaagcagcag ttgaagacgc acaacctaaa ggagcacttc cagaaggaac tgtggtcgga
     4501 gacggaaaaa tcaaatatgt attagcacgt gttgactcac gtctgttaca tggacaagtt
     4561 gcgacagctt ggacaaaagc agtgcaacca aaccgtatta tcgttgtttc agatgcagta
     4621 tcaaaggatg atctgcgtaa gagactaatc gaacaagctg ctcctccagg tgtaaaagca
     4681 aatgttattc ctattagcaa aatgatcgaa gtagcaaaag accctcgttt tggaaatacc
     4741 aaagcattgc ttttatttga aaatcctgaa gacgtattga cagctgttga aggtggcgta
     4801 gacatcaaag aattaaatgt tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt
     4861 aaagtattgt ctatgggaca agaagacgtt gaagcgtttg agaagcttga acaaaaaggt
     4921 gtgaaattcg atgtacgtaa agtacctaat gattcacgcg ataacatgga tgatattttg
     4981 aaaaaagcaa aagctgaatt agcaaaagcg taataaataa tattaaaatt tataggaggc
     5041 ttatcatgtc tattatttca ataattttag tcgtattagt tgcctttcta gctggtatgg
     5101 aaggtattct tgatgaattt caatttcatc aaccactagt ggcatgtaca ttgatcggtt
     5161 tagttaccgg taacctcgaa gctggtatcg tacttggcgg tacgcttcag atgatcgctt
     5221 taggttgggc aaatatcggt gccgctgtag caccagatgc tgcattagca tcagtagcat
     5281 cagcaattat tttagtttta ggtggacaag gtgttaaagg tgttccttct gcgatcgcga
     5341 ttgccgttcc tttggctgtt gcagggttgt tcttaacaat gatcgtacgt acaatcgctg
     5401 ttccaatcgt tcatttgatg gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa
     5461 tgtggcacat cattgctgta tgtttacaag gggttcgtat cgcggtccca gcagcagcgt
     5521 tgctatttat cccagcacaa actgtgcaat cattccttga atcaatgcca gcatggttaa
     5581 cagatggtat ggctatcggt gggggaatgg tcgtagctgt aggttacgct ttggtaatca
     5641 acatgatggc tacaaaagaa gtatggccat tcttcgttat tggtttcgtt gtagctgcaa
     5701 tttctcaatt aacattgatt gctctaggtg ctttaggtgt tgcattagct cttatttact
     5761 tgaacctttc taaaatgggt ggttcatcaa ataatggagg cggaagcaac tctggtgacc
     5821 ctttaggcga cattttgaat gactattaat cttgaaggag gaaaataaaa tggcagaaga
     5881 aaaaatcaaa ttatcaaaaa aagatcgttt agctgttgcg tggcgctcta ccttcattca
     5941 aggttcatgg aactacgaac gtatgcaaaa cggtggctgg gcattttcta tgattccagc
     6001 aatcaaaaaa ttatacaaaa caaaagaaga acgttctgca gcattaaaac gtcacttgga
     6061 attctttaat acacacccat acattgcttc tccaatttta ggggttacat tggctctgga
     6121 agaagaacgt gcgaatggcg cgcctgttga tgatgtagcg attcaagggg ttaaagtagg
     6181 tatgatgggt cctttagctg gtgttggtga cccagtgttc tggtttacag tacgtccaat
     6241 gctaggtgca ctaggtgctt cattagctat gggaggaaat atccttggac caatcatctt
     6301 cttcttaggt tggaacatta ttcgttgggc attcatgtgg tatacacaag aatttggcta
     6361 caaagctggt tctaaaatta ctgatgacct ttctggcgga ttgttacaag acgtaacaaa
     6421 aggtgcttca attttaggga tgttcgtatt agctgccctt gtacaaagat gggtatcaat
     6481 caaattctta ccagttgtat caactgtaaa attagataaa ggtgcttaca tcgaatggga
     6541 taaattgcct tcaggtggtg aaggaatcaa gagtgcattt gaacaagtta ataatggctt
     6601 agccctttct cctgaaaagg taacaacatt acaagacaac ttagaccaat tgattcctgg
     6661 tttagctgca ttaggattaa cattgttatg tatgtggttg cttaagaaaa aggtttctcc
     6721 aatcgttatc atccttggtt tattcgtagt cggtgttgtt ggacacgtca tcggactttt
     6781 ataatccata aaaatatagc tcgggcgttg tcccgagctt tttttatata gtatactgtt
     6841 cataaacaaa tatctggagg gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg
     6901 taattgatgc tacttcgttt acgggtctga cagattacgg gcaaatcatg ataggtgata
     6961 atggttttga gttttaccat gcgcgagata gacgcaaata tatccaaatt ccatgggaag
     7021 aagtggatta tgtactagca tcagtcatgc taaagggaaa atggattcct cgatatgcca
     7081 ttcaaacgaa aaaaaatggt acatacacat tttcttccaa agaacctaaa aaagtgcttc
     7141 gtgcgatcag agaatatgta gatgaagata gaatggtaca atcattgagt ttctttgatg
     7201 tagtgaaacg tggtattcgt agtatgttta aacgcaagta aaaaataaag aaaacttcta
     7261 agaggaggtt ttctttattt ttttactttg cagaagaaaa tatgaaatag caaaaccaat
     7321 tatttgtccc ctttaaatcc catatgctaa aattgattag caaagaagga ggttatatta
     7381 tgagttggtt atggacattg attgttggag ccgtgatcgg agcaattgca ggtgcgatca
     7441 ccagtaaagg aaattcgatg ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa
     7501 gtattggaca agcgattttc ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc
     7561 catctatttt aggcgcagtg attttggtag cagttgtctc atttttcttt ggaaaacgat
     7621 cataagtttt tacaaaatag taaactaaaa ggagttgcag cgagactgtg actccttttt
     7681 ggctttattt atttaaaata aaaagaagtt ttccggaaat ttttatttaa ctgtcgtgta
     7741 cgaagaaaag tttgctataa tggaaaaaag cgtgcaaaga aagagggagt ttcattatga
     7801 tgctacgtgt agaaaaattg cgcaaaaaaa tgcaagaaga aaatttggat tcatttttgg
     7861 ttaccagtcc gtacaatctt cgttacttaa cgaactttac aggtacgaca ggattagctg
     7921 ttattacttt ggaaaaagca ttttttatta ctgattttcg ttataccgag caggcggcag
     7981 cgcaggctca agggtttgaa attattaaaa atgtcggacc tatttttgat gaggtagcaa
     8041 acttagtacg caaagaagaa ctgcactcat tagcttttga ggaaacaaca gtctcatttt
     8101 tagaatattc ggttttagaa gaaatcatcg atgcggaatt agtacctgtt tcacagatga
     8161 tcgaagaact cagagaagtg aaagatgaag aagaaatcgc tattatcgaa aaagcatgtc
     8221 atattgcgga tatggcatat gatcacatcc tgaaaatgat ccaaccgggc atgactgaga
     8281 ttgaagtcgc aaatcaacta gacttttata tgcgttctct tggcgcttca ggtgtttctt
     8341 ttgatacgat cgttgctagc ggattgcgtt cggctatgcc gcatggtgta gcaagcaaaa
     8401 aaatcatcga acaaggtgac ttgatcacat tggactttgg ttgctattac gaagggtacg
     8461 tttccgatat gacacgtaca ttcgctattg gtgatcctgg ggaaaaatta aaagaaattt
     8521 atcaaatagt cttagatgct caattagcag ttattgatgc tgcaaaacca ggaatgactg
     8581 gtgtccaatt agatgcagtt gctagagatt atatttcaaa acatggttac ggggaagcat
     8641 ttggacattc tacaggacat gggatcggtt tagagatcca cgaaggacca aacgtttctt
     8701 ctcgtgcaga aaagcaattt gtaccaggaa atgtgatcac tgatgaacca gggatctact
     8761 taccaggaat cggtggagta cgtattgagg atgacttgtt gatcacagaa gatggaaatc
     8821 gtgtgttgac tcgttcacca aaagaattga tcatcttata gtttcatcaa tagaagaagt
     8881 aagcgtaaaa atcagtgttc acagactttg ttatagaaaa tcactcgatt taatgataca
     8941 ctatagaaga acttttaagc tgtaagagat aataaagttg ggtgttttct ccaattcata
     9001 gcagtttgca gctgatcggc ggcagttttc ttaccacgtg atcactatgc cgcatcatag
     9061 agaattcgga tgtatctcgt gcttaggcaa agggaggaat aaataaatgg cagatgaaaa
     9121 aaatttgatt ttaaacgcaa accaagattt aggagagatc gtgatcgctc ctgaagtcat
     9181 cgaagtaatt atcgggattg cagcttctaa agtagaaggc gtatacggaa tgcgcggaac
     9241 ttttgccagc aatgtcactg aattattagg acgtgcagct cacggaaaag gtgtttactt
     9301 agcaaacgag gaagatggct tgaaagtaga tctttactgt tatctagaat atggtgtttc
     9361 agttcctaaa gtagcaatgg aaatgcagga acgggtcagc cagcaagtcc ttttcatgac
     9421 ggatatcgat ttatctgaag tcaatatcca tgtagtagct gttgttcctg aaaaattaga
     9481 acaaccaaca atcgaagaac tttttgaaga cgaagaggaa gagaatgaat aaagaattat
     9541 caagacacga gattcgggaa atggcattgc aggctttatt tccattagac ttcaatgcag
     9601 acctaacaaa agaagatgcg attttcaacg caatcgaatt agaccaccgc gacatgatca
     9661 acgaagatga atcagagttt gttcctgttt atcttgatac gctagttggc ggtgtctgtg
     9721 caaaaaaaga tgaattggat aaagtgattg aaaaacattt aaaaaacaat tggcacatca
     9781 atcggatctc aaaaatggat ttggtgatct tgcgtatcgc gatttttgaa atgacttatg
     9841 tgacagatgt accagcggct gttgctttaa atgaagcaat cgagttggca aaaacattta
     9901 gtgatgaccg ttcacgtaaa ttcgtcaacg gtgttttatc caatgtattg aaagaattag
     9961 aagctggggc gtaagtccta gcttttttct atgaatcatc ataaacctgt tataaatcgg
    10021 gaaaattcta tggtagaatg aaaacacaaa aaatatctgg gagtggttag atggcagaat
    10081 tgattaacgg aaaagaatta gctgaaaaaa tgcaggcaga gattgctgaa aaaatccaaa
    10141 aattgaaaga taatggtatc catcccggat tagtcgtttt attggtaggg gaaaatccag
    10201 ccagccaaat ctatgtaaga aataaagaac gtgtagcaaa agaaatcgga atccattcat
    10261 tggtagagcg ttatcctgag acgatttctg aagacgaact acttgcagaa attgaaaaat
    10321 acaatcaaga tcctagtttt catggtatat tggtccaatt accattgcca aagcatatag
    10381 atgaagaaaa gatcctgtta gctatccgcc ctgaaaaaga cgtagacggt tttcatccga
    10441 tgaatttagg cagactcctt gcgggtaaac cggacaagat tccttgtact ccatacggca
    10501 tcatgaaaat gtttgaagct tattctatcg atccggcggg taaacgagca gtagttatcg
    10561 gacgaagcaa tatcgtcggt aaaccgatgg ctcagctact cttgatggca gatgcaactg
    10621 ttactgttgc tcattctaaa accgaaaatc tggctgaact ggcacaagaa gcagatattt
    10681 tagtcgtagc aataggacgt ggacattttg tcacaaaaga attcgtaaaa ccaggtgctg
    10741 tagtgataga tgtagggatg aatcgtgatg ctaacggtaa gctgatcggt gatgtgaaat
    10801 ttgatgaagt ggagcctatt gcaggctgga tcactcctgt gccaaagggt gtagggccaa
    10861 tgactatcac gatgctgatg tatcaaaccg taaaatctgc cgaagctttt ggagcaggtg
    10921 aatagatggc acaagaatat ttaactgtca ctacattaac gaagtatttg aaaagaaaat
    10981 ttgatgccga tccttattta gaacgcgtat atttaacagg cgaaatatcg aatttccgtt
    11041 tacgtgccaa tgcccatcaa tatttcagct taaaagatga ccatgcaaaa atatctgcta
    11101 ttatgttcaa aggagcattc caaaaattac gattccagcc aaaagaagga atgaaagtcc
    11161 ttgtcgttgg gcgtatttca ttatatgaag cgggaggttc ttatcaaatc tatgtagagc
    11221 acatggagcc tgatggaata ggtgctttat atcaagaatt ggaagaacga aaagaaaagc
    11281 tgatgaagga aggccttttt caagggccta aaaaaatgct gccacgtttc ccaaagcgaa
    11341 tcgcggtcct taccagtcca agcggagcgg ttatccgcga tatcatcacg acggtgaaaa
    11401 gaagatatcc aattgtccaa ttggttttgt tcccaacagt tgtacaagga aaccaagcag
    11461 cggacaatgt tgtcaggaat atccagagag tagaagagat gggcaatttc gatacaatga
    11521 tcatcggtcg tgggggtggt tcgattgagg atttatggcc gtttaatgaa gagcgggttg
    11581 ctcgggcgat ttatcaagct aatacgccgg tgatttcatc tgtaggacat gaaacagata
    11641 ccacgatcgc tgatttagta gcagatgttc gggctgccac tccaacagca gctgcagaat
    11701 tagctgttcc tgttttgtca gaagagttga tgcgcattca agaacggcaa gctagattgc
    11761 agcaggcgtt tgtacgccag atccagcgca aacaagaacg gttcgagcgt gctcgacgtt
    11821 cctatgtatt tcggcagcct gaaagattgt atgaaggtca gaccatcaaa ttagaccagt
    11881 tgaaacagcg attatatcaa gctacacagc aaatttatca tgcgaaagaa aaacaaacga
    11941 tcgcattaag ccaccaattg gaacaagtgg ctccgcttta tcgagttaaa gcagctcgcc
    12001 aagaaactgc ttatctagaa aaacgcttag aagagaaaat ccaacaatat atgaaacaac
    12061 agcagcagcg ttttgctcaa gctgttcaat ctcttgattt gttgagtcca ttgaaaatca
    12121 tgggaagagg ctacagctat acgacgttaa atgatcaagt agtaaaaagt gtcaccgaga
    12181 ttcatcaagg ggatttgctg acgatccatt atcaagatgg aatcgttaaa ggacaagtac
    12241 aagagataga caaaggagaa taaaaatatg gaaaatccaa cttttgaaga gtcactgcaa
    12301 gaattagaaa aaattgtcat gcagttagaa caaggagacg ttccattaga atcagctcta
    12361 gattcgttta aacgaggaat ggaattaagc aaacattgcc aagatacatt agctaaagca
    12421 gaagagacct tgacgaaaat gatgacagaa tcaaatgaag aagttgattt tgacggaaac
    12481 gaggaaagct gagatgagat taactgactt ttcagctatt catctacctg ctgtagaaaa
    12541 agaaatcttg tcttttttag atgagcatac aacggataga ggactctatg atgcgatgag
    12601 ttattcagtg aaagcaggcg ggaaaagaat acgtccctta ttacttttaa cggccgttgc
    12661 ttcctttgat gagcctattg atgttcccgt ctatcaagta gcagctgctt tagaaatggt
    12721 gcatacgtat tccttgatcc acgatgattt gcctgcgatg gacaatgatg atcttcgtag
    12781 aggaaaacct accaatcata aagtattcgg cgaagcatta gctatattgg ccggagatgg
    12841 tttgcttaca ggagcattcc agctaatcag tatggcacac ttaggtaatt ctcctaagct
    12901 tctgctgctt cagcaattag ctgtttgtgc gggatcacaa ggtatggttg ctgggcaagc
    12961 tgcagatatc gagggcgaat cgaaaaagct ttctttagaa gaattagcat ttattcatga
    13021 aagaaagaca gggcatttga ttcgttatgc cttgctggct ggtggcatat tagcgaagca
    13081 acctgaagaa atcttattgc tacttcaacg attagccgaa catttaggtt tagcttttca
    13141 gataagagat gatctactcg atgtgattgg tacaacgaaa acattaggga aaacagctgg
    13201 taaagatgaa cgtatggaaa aaaatactta cccgcgttta ttaggcctag aaaagacacg
    13261 tgaggcgttg gagattgagc tgttatccgc caacaagatc atagacaaat tagaagagga
    13321 cgttttttct tttgatggcg ggctgatgcg ccagatgatc aagcaatttg atgtggagaa
    13381 atgagggaaa gaatggaaaa agaacgcgta gatgtattag cggtcaaaca aggattattt
    13441 gaaacaagag aacaagccaa acgttcagtg atggctggtt tgatttacaa cgaaaaaaac
    13501 gagcggtttg ataaaccggg agaaaaaatt ccagtgagta gtgaattaag agtcaaaggt
    13561 aaaaaacttc cttatgtttc tcgaggggga ttaaaattag aaaaagcact caaagcattt
    13621 gatctatctg ttgaaggaaa aacattgtta gatattggtg catccactgg cggatttact
    13681 gatgcagctt tgcaaaatgg cgcaaaaatg agttatgctt tagacgttgg ctacaatcag
    13741 ctagcttgga aaataagaca agatcctaga gtcgtcgtaa tggaaagagt gaattttcgt
    13801 tatgcaaaac ccgaagactt taccttagga attccagaag ttgctgttat cgatgtttct
    13861 tttatttcac taaaactgat gctgcctcca ttacatgcta ttttaaaaga agagggagaa
    13921 gtgattgctt taatcaaacc ccaattcgaa gcaggcagag aagctgtagg gaaaaacgga
    13981 attgttcgtg accctcagac ccatcaaaaa gttttagaag acattttgtc atttgcggca
    14041 caaggtggat atgatgtgct ggaattaagt tattctccga tcacgggggg agaaggaaat
    14101 atcgagttct tggctcactt gaggaaagta cccgaaagcg gaacaatcaa ttcagctatt
    14161 aatatggcag aagtggtttc aaatgcacac gaacagtttg accataaata aattgtaact
    14221 gtgaaaatca agtgagaagt atgcggattc ttctttatta taccaaggaa ataagctatc
    14281 attgattaag aagaatatgc taaggagcga agacgtaatg agaaaaaaag atcggcatcg
    14341 tttgattact cgattgttga gtgagcaaga tatccgaaag caggaagaat ttgttgaaat
    14401 cctgaaaagc aaaggaatat ctgttactca agctacgatt tccagagata taaaggaact
    14461 gaaactgatc aaggtaccgg cttcagatgg cggctaccgt tatagcatac cagctgaaac
    14521 aaacgaagat gtaaatacta aattggaaaa actattaaaa gacgctttcg tggcagttga
    14581 ccaaatggaa aaatttgtta ttttgaaaac attgccggga aatgcttcgg cagcagcaaa
    14641 tttgattgat aaacgttaca agaaagaatt attttcgatt atcaatgatg acgataatgt
    14701 tttgatgatt acacgaactg aagaccaggc agttttattg aagaaagact tcctgcatta
    14761 cctttaagtt gaggagcgaa tgtgatgcta caagaaatca gtattactaa ctttgcgatt
    14821 attccggaat tgcggctttc ttttcacgaa ggaatgaccg cgctgaccgg agagactggt
    14881 gcgggtaaat ccattattat cgatgcttta ggtctgttag caggaggaag aggttcaagc
    14941 gattatatac gtcaaggagc agaaaaatgt gtgcttgaag ggttgtttga actaccaaaa
    15001 caagaaggat tctctgaact gatggttgaa ttagggatcg aaacagatga ggacaatctg
    15061 attgttcgac gggatatgtc cttaactgga aaaaatgttt gtcgagtgaa tggtcatatt
    15121 atcacattag ctaatctgag aagaatcggc agttatttag tagatatcca aggacaaaac
    15181 gaacatcagg aactgctcca gcctgaatca catcttgctt tattggatcg ttttggagat
    15241 gtagctttcc aacagaagaa aaagagttat cagcaagaat atatctctta tcgtgagctg
    15301 gaaaaaaggg tacgaaaagt gcagcaaaat gaaaaatcct atgttcagag aatagatatg
    15361 ctccatttcc agcaagaaga aatagctgct gctgatttgc aagtcggaga agaagagaaa
    15421 ttaaaagaag aaagagaaaa acttagcaat tatcaaaaaa ttgctgatgg attagctgca
    15481 ggttacggag cactgacgaa taatgaacaa aacagtgtag atggtgttgg cttggccgtt
    15541 tcagaactgc aagggatcgc ccatctagat gtggagtatg aagcaattta tgaaaatatc
    15601 caaagtgctt actatttgct gcaagatgca atcggcgata tgagtcgtca gattgattta
    15661 ttggagttag atgagagtcg tttagaagaa gttacgcaac gtttagaatt aatccgacag
    15721 ttgaaacgta aatacggcga atccattgag tcaatattgg cttactatga cgaaatcacc
    15781 gaagagctag cttcttctga tttttcagaa ggtcaattgg acaaaatgaa agaggaacta
    15841 gagcaaaaag agcttctact tcagcaacaa gccgcagatc ttcatgaagc gcgtaaaaaa
    15901 atcgcaaaag aattggaaaa atccattctg catgaattga aaagtctgta tatggaaaac
    15961 actgagtttg aggtacgttt cttaaaagaa gaaaatagac aattgaatca tgatggattt
    16021 gaccaaatcg aattttatat tacgaccaat ccaggagagc cactgaagcc tttggtgaaa
    16081 gtcgcttctg gtggcgaact gtctcggatg ttgcttgctt tgaaaactat tttttcttct
    16141 gagcaaggtg tgacaagtat tatttttgat gaagtggata caggtgtgag cggaagagtt
    16201 gcacaggcca tcgcagataa aatttctcaa atatcgaaat actctcaagt tttgtgcatc
    16261 actcatctgc ctcaagttgc tgcagtcgcc gactatcaat attatattgt gaaagcagtg
    16321 attgatggac gtacgcaaac ttctgtttca gagttgaaaa caaaagaaag agaagaagaa
    16381 attgcacgta tgctggctgg aagtgagatt acaaaactca cactcgaaca tgcaaaagaa
    16441 ctattgaaac tcgcaaaaag gacctgagcc acctcgcagt ggttcaggtc cttttcagca
    16501 gctgatagct atattttatt tttacatgac actcattaca gcagtcaata caacagatcc
    16561 aatcgttaaa atcagcattg tccaaatgac aaattttgtt actttagcaa aagtacttgt
    16621 ttgttttttt tcacgcattg ttttcaacct cgtcattaat tagttgattc aagtgtacaa
    16681 tcttttttcc atttccacaa gagggctttt ttataaaaac ttcccttttt ttaaataata
    16741 ggtagttacg atacaaataa ataatgttaa tagaactagc catataaagg catcatccct
    16801 ttcagcaata ggtaacttaa tgttcattcc atagatactg ccgacgattg tcggtatagt
    16861 taaaataatc gttaaagaag ttaaaatttt cataatatta ttcagattat tcgatacgat
    16921 tgctgaaaaa gtctgattca tttgttctac tagttttaat tgaatgcgag aagtagtcat
    16981 tgcctgctct gtttccacca atacatcacg taaatgattt ttcaaagcat gattttcttg
    17041 gaaaagttta ttttgcgcta gttctttcaa tgcatctaaa ttagcagaag tcgcttctct
    17101 gaaataaacg aggctttttt ggatatccat gatttgataa agttgtttgt tttcagtgga
    17161 tacttgaagt ttggcttcca aagtatcgca ttgagattga agcgtagcta aataactgtt
    17221 gtaagagagt gtgacctgcc aaatgaattg aaagaagata ttgagattag gcgaaatatc
    17281 actttttgga aaggtctggc taaagacttt ctttaaaaac agtggttcat gattcgtaat
    17341 tgtgataagc ttctttttgg gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa
    17401 ataaccgcta ggacttgttt tagcaaaagg atattgtaac aataataacg ctggctttgt
    17461 caactttgat tgattgaatt cttctgtacg ggaattttct gcatcgtcca atatggaagt
    17521 gatgtaatct gacggaattt caaattgatg tgtcagctga tgaatctctt cttgattagg
    17581 acgttctaca caataccaga tcgtttggtc agtttccttt ccggtattac gcagtttctc
    17641 attatctaat gtaaaatatt tgatcatgat tttcctcctg ttgcctgttt cttttattat
    17701 aacggatagg gaattggaat acatggaatg tgtttgtaag atatagaata taactgcata
    17761 aatttaaata aaatgaaaca gttgtgtaaa gtatatacag tatacttgaa gcaacgataa
    17821 aaaaggagtt tacgttcatg ggcacattca caccacttat cttgttttgg tatatttacc
    17881 cggtaatcgt tttgtttggt tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt
    17941 ttaaaataaa agcccctgat ttggcgattc cttttttgtg gggcggtatg catgcattgt
    18001 ctagaaatac agggacaatt tctatcttac cgtttttctt tatttctgtt ttgctaatgg
    18061 gaatttgtat tgctgttttc caagcatatt attatgaaga aattatttat ccgcgttatt
    18121 tcaagatgac ttggcgtttg gtttttctgt tgacaatcgt cttgtacgct gtcttgatca
    18181 ttgtgaacat actttcttac ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa
    18241 ggctttttgc agcttgattt ttaaaaaaat gtttatgaat aaaaaaaagt tgttcccgga
    18301 tttcccagaa aaggtggtag aaagtggggg attgtggtag actaatttta gttagtggag
    18361 gaatgggggg ctttcaatgt tgatgggtga attccaacat aatattgatg caaaaggacg
    18421 tttaatcgtg ccctcaaaac ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg
    18481 attggatggc tgtcttttcg gctatccgat gagcgaatgg gaaaatctgg aagctaaact
    18541 gaacgaaatg ccgttggcta aaaaagacgc acgtactttt gtgcgattct tctattcggc
    18601 tgccactgaa tgcgaacttg ataaacaagg acgaatcaat atacccagca cgttaagaaa
    18661 ttatgcagct ttgacaaaag gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg
    18721 ggatgagaca cgatggcaag atttttctgc agcagcagaa gagaactttg acgaaattgc
    18781 tgaaagtatg attgatttcg gactataaga ataggaagag gctgactaat gactgaaaca
    18841 tttcaacact atacagtcat gttgaaagaa acggtagatg gattgaacat caaaccggat
    18901 ggcgtttatg tagactgtac actcggcggt gccggacata gtgaatattt attaagccaa
    18961 ctaagttcta aagggcatct gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc
    19021 aagctgcgtt tagcttctta tgtcgaacaa ggtatggtca catttatcaa agcgaacttc
    19081 agagaattag aagaacgctt gagtgagcat gtagataagg tagacggcat tttgtatgat
    19141 ctaggggttt cctctcctca gctagatgaa gcagaacgtg gatttagtta tcatcaagat
    19201 gcaccgttgg atatgcgcat ggatcagtca gctcctttat ccgcttatca cgtaattaat
    19261 gaatattcct accatgaatt agtaaagatt ttcttccgct atggcgagga gaaattttcc
    19321 aaacaaattg ctcgtgaaat cgaacgagta cgaaaaacaa aaccaatcca aacaactggt
    19381 gagcttgtcg aaatcattaa acaagtcatc ccagctcccg caagaagaaa aggcgggcat
    19441 cctgcaaaaa gaatttttca agctgtgcgg attgcagtaa atgacgaact cggtgccgaa
    19501 gaggcctctt tagaacaggc aatccggtta ttgaaagtca atggaagaat cagcgtaatc
    19561 acattccatt cattggaaga cagaattgtc aaaagtatgt ttaaagaata tagtacggta
    19621 caagatctac caccgggact tcctgtcata cctgacgaat tccaaccaga actgaaagtc
    19681 attactcgta aaccaatcct tccttcagag gaagagctaa cagaaaacaa tcgatcacgc
    19741 agtgcaaagt tacgtattgc agaaaaaata agaatgaaag aggagtagaa tgatggcaga
    19801 attaaagaaa atgcaagatt acgaatatga tttacctgaa ttggtggatc agccggaaca
    19861 acaaatgcct gattcttctt cgaatcaaga aatcattctt ccacaatcac cggcaaaacg
    19921 attaaagcag atttctgctt tggaaaaaat tgctgtggca agcgtggcga ttgctattgt
    19981 tgctttatgt gttttaacag ttatgctgcg cacaaatatc agcggagctg aaaaagaaat
    20041 cacgtcaata caaacagaaa tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga
    20101 aaaaaacgaa ctttctcgta ctgaacggat taaacaaatc gcagaagaaa aaggattgtc
    20161 tattaatgac gacaatttaa ggataaggaa agtgaagtaa atgagtctta aaaataaaat
    20221 tcggcggttt atggaaaaga aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat
    20281 cattttattt gctacgagta ttggattgtt ctttttattt gccgtccgtt tctcgtatat
    20341 cgttattgga ggtcatgttg ctggtacctc attagctgaa aaaacaaaac agttgtacca
    20401 aggaagtgaa gtagtaaaag caaaaagagg aacgatttat gatcgtaacg gtgtggcgct
    20461 cgcagaagat gcctcctctt attcaattaa agcgatactc tcaaaaacat atacctctgg
    20521 ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa atagcagaaa tcctgcataa
    20581 gaatctttct atagacaaaa aagatgcact gaatatttta gaggacggcg caaaaaaaga
    20641 gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa
    20701 catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt tattttgtcg accatcaggc
    20761 tcggatgtat ccaaatggcg tcttttcttc tcattttatt ggatacgcag ttccagacaa
    20821 agatgagaat ggcttagttg gtaaactagg tttggagtct gcttacaatg atatcttgag
    20881 cggtaaagat ggtaagatta tttatcaaaa agataatttt caaaatcctc tgccaggtac
    20941 agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc tacacgactt tagacagcag
    21001 actacaaagt tatttagaaa cactgatgga tcaagtaaat gaagaatatc agccagaaga
    21061 attgacagca gtcctgatga aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc
    21121 tactttcaat cctgaaacaa tggaaggatt gactggaaaa gatgctatct ggcgtaattt
    21181 cttggtacaa gatagttatg aaccaggatc aaccatgaaa gttttcacta ctgcagctgc
    21241 tatcgaagaa ggagaattca acgagaatga aaccttccaa tctggaaaaa tccaagtaga
    21301 agatgctacg atcaatgacc atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc
    21361 tctttcatgg tcaagtaacg tcgggatggt catactggaa cagcgattag gcggacgatg
    21421 gtataactat ttacaaaaac tgggatttgg acaaagtact cattctggat tagatgatga
    21481 agtaaacggt gctttaccta cattaaatat tgtcgaccga gcaatgagtg cttatggaca
    21541 agcggtaggt gtgacgaatt tccaaatgat gaaagctttc acttctattg cgaataacgg
    21601 tacaatgatt caacctcgat atatcagcaa agtggtggat ccccaaactg gagaagagcg
    21661 aacaactcaa acagaagttc tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg
    21721 tgaatatatg agagatgttg tcgaaagcga aaactacggt agtgcttatg gtgtatatag
    21781 tgtacctgga tacaatgtat cggcaaaaac cggaacagcc caaatcgctt ctgatacagg
    21841 gggatatcaa acaggagata cagcctacct gtattcgatt gtggagatgg ttccttctga
    21901 agatccagac tatgtcttgt atctaacaat gaaacaccct aagacatatg atcgaatggc
    21961 attggctaaa atcgcaaatc cattgatgaa acgggcaatg gattttaaag aaactgaaga
    22021 agattcagat acagaaacaa aaacagaaaa agtatccgta gccgattatc gtaatcttga
    22081 agcggacgtt gcagcagctg atgctcaaaa aagtggactg caacctgtag tgatcggtaa
    22141 tggtaaaaaa gtacaaaaac aatctactgc caacggtgat cagttgatct ctggagaaaa
    22201 actgatccta tatactggtg gtgataagct gatgcctgac gtaacaggat ggtcaaaagc
    22261 agatatcatg aagctgggga aaatcctcgg tattgaagtt agttttgatg gagatggtta
    22321 ttgtgtcaaa caagaacttg ctccatatga aaaaatcact gaagacaaac ttagttttac
    22381 tttagaagaa taagaagaga aataggagag aaaagagcat ggaatggaca caagcattga
    22441 ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat gccgctattt attggttatt
    22501 ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg accaaagtgg cacaatagca
    22561 aagccggaac tcctacaatg ggcggcttag tgtttctgat cggctctatt ttgacaggaa
    22621 tttgggttgg agcttggcaa aaacagctaa ctcctactct tttcatcttg ctttttgtat
    22681 tagcgttata cggagtaatc ggctttcttg atgattttat aaaaatattc aagaaaagaa
    22741 acatgggact gaactcgaag cagaaattga ttggacagat tctaggggga atcatctttt
    22801 atcttgttta ccgttctgaa ggttatccag gtgtattgaa tttcttcgga ctagatctac
    22861 ctttaggaat catttatggt attttcgctc tcttctggct tgttggattt tcaaatgcag
    22921 taaatctgac agatggcatc gatggattag ttgctggctt aggaagtatc tcttttgcag
    22981 cttacggtct aattgcttgg catcagcatc agtatgatgt attaatcatt tgcttaagtg
    23041 ttttaggagg tttactgggt ttcttcgtct acaacagaaa acctgccaag atttttatgg
    23101 gagatgtcgg ttcattagcg ttgggcggac ttttagcggc aatttctata atgctaaacc
    23161 aagaatggac attgcttttg attggtttga tctatgttat ggaaacagca agtgtgatgc
    23221 ttcaagttac ttcctttaag cttacaggaa aacgaatttt taaaatgtct ccaattcatc
    23281 atcactttga aatgtgcgga tggtcggagt ggaagattga tacagttttt tggttgatca
    23341 gcattgtgac ttcgttgatc acattatgga tcgtttggta ggagatgacg ggaaatgaaa
    23401 aaaatatcaa catatgaaaa taagaaagtg cttgtcttag gactcgcaaa aagcggagta
    23461 agtgcggcca aattattaca tgaattgggc gcacttgtca ccgttaatga tggaaaacct
    23521 tttgacgaaa atccggaagc acaagagctg ctttctttag ggataaaagt aataacaggt
    23581 agtcatccaa tcgaattgtt ggatgaagaa ttttcattga tggtcaaaaa tccagggatt
    23641 ccctacagtc atcctttagt agcaaaagcc caagaaatgg gtattccggt aattacggaa
    23701 gttgagctag cttacgaagt agcggaatgc ccaattattg ggattacagg aacgaacgga
    23761 aaaacaacga caacaacgat gacaggacta ctactgaatg caggagcaga tcaaggaatt
    23821 gcacgtttag cgggtaatat tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca
    23881 gaagataaaa ttgtgatgga attgtctagc ttccaactca tgggaatcac agatttccgc
    23941 cctcatatag cggtgattac caatatctat gaagcacata ttgattatca tggtacaaga
    24001 aaagaatatg tcaaagctaa atggaacctt caaaaaaaca tgacagaaaa agattatctg
    24061 atccttaact ggaatcagtc agaactacaa gaattagcgc aaagaacaaa agcacgcgta
    24121 ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt atgctgacga ttactcaata
    24181 tactataaaa aagaaaaaat aatggaaata tctgaacttg gcgtaccagg taagcataat
    24241 gtagaaaatg cactagctgc tatttctgta gcaaaattgt atggtatatc aaatgaagct
    24301 atacgagaaa cgttgcattt tttccatggc gtaccacatc gtacgcaata tgtaggagaa
    24361 atccaaggca gaaaatttta taatgattca aaagcaacca atattttagc gactaaaatg
    24421 gctctaagtg gatttgaaac gtctaaggtc gtgttattgg ctggcggatt agacagagga
    24481 aatacatttg atgaattaat tccttcatta aaaggaatca aagcaatggt cgtatttggc
    24541 caaacaaaag aaaagttgat ggatgctgga aaaaaagcag gcatcgagac aatagtaaca
    24601 gccgattctg tagaacaagc agttccttta gcattagaaa acagtacaga tggtgatgta
    24661 gttttattat caccagcgaa tgccagctgg gatcaatacc caaattttga aactagagga
    24721 aacagattca tggaagctgt gaaccgatta aaatagaaaa agtagaagcg agcgataaaa
    24781 aatgaaaatt ttggttacag gcggaggaac agggggacat atttatccag ctcttgcctt
    24841 tgtcaattat gtaaagactc aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg
    24901 aggtctagaa aacaaaatcg tccctgatac aggaatgcct ttccatacct tggagattca
    24961 aggatttaaa cgaaaaattt ccatgcataa catcaagacg gtccgattgt ttcttaagag
    25021 tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca gatatcgtta tcggtactgg
    25081 aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa atgggtattc caacagttat
    25141 ccatgaacaa aacagtgtac caggtgttac caataaattt ttaagccgat atgtggatcg
    25201 gatcgctctt tcatttaaag atgcggcttc cttttttcct gaaaacaaag cagttcttgt
    25261 aggtaatcct cgagcacaag aagtagcaga tactaaaaaa tcagatattt tgaaaacatt
    25321 tgatttggat ccagaaaaga aaacagtctt gatttttggc ggtagtcaag gagcacttaa
    25381 aatcaatcaa gcagtatcag tttttttaag tgaatatatt ccttcttctt accaagtttt
    25441 gtatgcttca ggagatagat attatgaaga gatcctaaat aaaataaatc ctgaaataca
    25501 aaatattagt gtccagcctt atatcaaaaa tatggcagag gtcatggctt ccagtgatct
    25561 gctggtgggg cgtgctggtg caacttcgat cgcagaattc acagctctgg ggttaccggc
    25621 agtgttggtt cccagcccat atgttacgaa tgatcatcag actaaaaatg caatgagtct
    25681 tgttcacgca ggagcagcaa aaatgattgc agataatgaa ctaacaggtg aatcactaag
    25741 tcagacagtc aacgaaatca tgggtgatga agaattgcaa aagcaaatgt gccgagcttc
    25801 taaagaacaa ggaatccctg atgcttcaaa acgtctttat gacttagtaa aacaaataat
    25861 caaaaaataa aggcggtggt cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat
    25921 ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg ctaaccgaaa atatctggca
    25981 gaacaagaga tgaaaaacca agagccaaaa aaagaagaca taacattaga tacacccctt
    26041 tcagattctg aagaagaaaa aacggttcaa gaggaacagc aagaagaaag ccagtccgag
    26101 aaaaaaatcg aattttttga agagatcaag caaaagaaaa acggtggacc atataacggt
    26161 tcttttttga accggctgcc caatttaaaa agtcaacggc acaaagtact atttcgtcgg
    26221 ttagctttga tcatcactgt attgggtata ccgctgattt ttttgatcta ttatgtttct
    26281 ccttatagca aattacaagc agttgcagta tcaggcaata agacagtcaa cagccaagaa
    26341 atcatctctg atacaaaatt gtcattagga gaaaatgttt ggggacagta ttttcatcga
    26401 tctacttata tcgatcgcct taaaaaagca cagccaagaa tcgaaacagc aaatatacat
    26461 ttcaaaggga tgaatgaatt cgatttggat gtaacagaat ataaagagat tgctttgatc
    26521 gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa cagtattaga tgaaaaagta
    26581 gcaaatccta caaaaaatct ccctatcttg gaagagttca aagactctgc aaaaatcaaa
    26641 gaattaacaa agcaatacaa tcagttatca tcagaattac aaaaagcaat ttctgaaatc
    26701 aagtacacgc caagagcaag caataaaaat ctgatccaat tgaatatgaa tgacggcaat
    26761 caagttattg taaatatcaa taatcttgcg aaccaaatga aatattattc gcaagtagcc
    26821 aaagatatgg atgaaaaagg tgtgatcgac atggaagttg gtatcttctc gcatccttac
    26881 ggtagcactc aagaaacgga agaatcgggc caatcggaag aaacaactga aagtgaagaa
    26941 aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct
    27001 ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt caagtgaaac aagtcaaata
    27061 gaagataatc caagtacaac tccgtcaagt ttaaatgaaa attgataaaa caactttaaa
    27121 aagtaagaat gtactttcta aaaaccagta atttatggta gaattggaag cagtgaatat
    27181 atctattaat aagtatattt taaaacggac gctagttgtt cgcttgtata tggaatagta
    27241 agtaggaggg aatcccattc atggcaaaaa caggaatgta tgtaggcctt gatattggaa
    27301 cgacatctgt taaagttgtc gtggctgaat atatcgacag ccaaatgaat attattggag
    27361 taggaaacgc aaaatcagaa gggattaacc gaggcattat cgttgatatc gataaaacgg
    27421 tccaagcgat acaacgagca gtacgacaag cagaagaaaa agcaggtatc cagatcaaag
    27481 gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga aaattgccag ggaatgattg
    27541 cagttaacgg agattcaaag gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg
    27601 cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc aatattgcca caagatttta
    27661 ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat gatcggcgta agattagaaa
    27721 tgtacggtct gttgtttaca ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg
    27781 aaaatgccgg attaatcgtc aacgaaatgg tgattactcc tttagcgctg actgaatcaa
    27841 ttctttctga cggagaaaaa gattttggta cgatcgtcat tgatatgggt ggcggccaaa
    27901 caacaacggc tgttatgcat gataagcaat tgaagtttac caatcttgac caagaaggtg
    27961 gcgaatttgt cactaaagat atctctattg tattaaatac gtcatttaac aatgcagaag
    28021 cgttgaaaat aaactacggg gatgcatacc cagaacggac atcaccagat gaagaatttc
    28081 cagtagatgt gattggtcag tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa
    28141 ttatttcagc tcgaatggaa cagattttca acaaagcaaa agatgcattg gaccaaatcg
    28201 aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg
    28261 ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca
    28321 tggggctgag aaacccggta tttacaaatg taataagtat cgtggattat tcagcaaact
    28381 tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc
    28441 aaatggtagt tgaacaagaa acaacaaaca catatgagag ctatgaagca cctgaagaaa
    28501 caatctacga tgagccggaa ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg
    28561 gtttctttac aaatattttt gattaattca tcctgggagg aataagatat ggaattctca
    28621 attgataata atatcaatga cggcgcagta atcaaagtca tcggagtcgg tggtggcggt
    28681 ggaaatgctg taaaccgaat gattgaagaa aacgttaaag gtgttgaatt cattacagcc
    28741 aacacagacg tgcaagcatt aaaaaattca aaagctgaaa cagttatcca attaggcccc
    28801 aaatatactc gtggtttagg tgctggttct cagccagaag ttggacaaaa agcggcagaa
    28861 gaaagtgaac aatccttacg cgaagcttta gacggtgcag atatgatttt catcactgca
    28921 ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa
    28981 ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca cttttgaagg accgaaacgt
    29041 ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa atgtggatac attattaatt
    29101 atctcaaata accgtttatt agaagttgta gataaaaaga caccgatgct tgaagctttc
    29161 cgtgaagcag ataatgtttt acgacaaggt gtacaaggta tctctgattt gatcacagcg
    29221 ccaggttacg ttaaccttga ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca
    29281 gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa
    29341 gcaatctcct ctccattgct tgaaacatct attgatggag ctgaacaagt acttcttaac
    29401 atcacaggcg gtttggatat gacattgttc gaagcacaag atgcttcaga tatcgtagca
    29461 aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat ctatcaatga agaaatgggc
    29521 gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag
    29581 tctagccgac cagctagaca agcacaaatg caatcacctg ctcaaaagac tgtattagat
    29641 atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca gttttggcga ttgggatatc
    29701 agacgtgaac aaaatgttcg tcctagagtt gatgattcca attttgacaa tatcgaaaaa
    29761 aaagaatttg atacattcaa ccgcgaagaa acaaaatctc atggcgatga tgaattaaat
    29821 acgccaccgt ttttccgtcg taaaaaataa aggagaggaa gagcacatga ttgctgacaa
    29881 cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa
    29941 agaagttaca ttagtagctg taacaaaatc tgttgaatcc gctcaagctg aagaattggt
    30001 tagagctggc gttatgaatt gtgcagaaaa ccgagtagat aaattattag aaaagaaaca
    30061 agatttattt gattttcctg aaataaagtg gcatttaatt ggtaatttac agcgcagaaa
    30121 ggtaaaattg attgtaaatg aaattgatta ctttcatgcg ttggacagtt taagattagc
    30181 agaagaaatc cagaagcggt tagagaaaga attatcctgt tttattgaag taaatgtttc
    30241 tggtgaggaa tcaaaacatg gaatttcacc agatgagctt ttaccgtttg tggaatcttt
    30301 agctgcctgc acaaaaatca aggttgttgg gttgatgact atggcgccta aagaagcttc
    30361 tgatgagcaa atccataaag tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc
    30421 aaaaggttat gcccatgcac catgtagtga attaagtatg gggatgagtc aagactttca
    30481 aattgcgatt gaagaaggtg caacatttat ccgtgtagga acagcgctat tcaaagacga
    30541 ataaaggagg aacaaaaatg ccgcttttaa acagaggagc aattgctaat tttttcggat
    30601 tagctgatga ggaagagtac gaataccaag attatcctga aacaacaaaa aaggctgtac
    30661 agccaactaa aaaaaataca acacagaatc aatcaactca ttctgtatct gctgcgaata
    30721 ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac gcatgaaagt attcagccgg
    30781 ctgaaaagaa aaaagtaatt gaactacatc aatcacctac aaaaactgta gcgaaagaaa
    30841 gaacagcttc aagaacctct acacaaccta aagcaaatct tgctggaaaa atcactgtaa
    30901 tggaaccaag agcttattct gaagctatgg cgattgctaa acgaatcatt gctggagaag
    30961 caaccttagt gaattttcat ttagtagaag aaaaacaagc gagaagaatc gttgattttc
    31021 taacaggaac agtttatgca ctagatggag atatcaaacg tgttggagat gagatttttc
    31081 tttgtacacc gactgggatg gagattgaca gttcaaccgc tcaatctttt gcagatgcga
    31141 atttgtttga tctgtaagag gaggaactac gatttatact attgcactat tacttcattt
    31201 acttcaacgg gcagcctatt tctatagcat tatcttgatg gtatatgcat tgctctcttg
    31261 gtttccaggc gggtatcaat cagggctagg acgattatta gctaagatct gcgaaccgta
    31321 tatcagtttg ttcgatcgtc tgcctttgag gattggaccc ttggatttca caattttagg
    31381 ggcaatcctg atcttgaact tagccgtaca aggtttagca cagatcgtta tatggataac
    31441 tgtccattta gtttattgat tgaagcaaca aaatctttat tctcagaata gataggaggg
    31501 gaaaattttg gatgcaaatg tataccagca ctttcgggca gatgaacgta catttatcga
    31561 ttcggtaggc gattggattg aacaggttca aggacaatat gcaccctatc tgaccgaatt
    31621 tcttgaccca agacaatcct atattttaga aacactgatt cgacaaagca gtgatttgcg
    31681 tttcatgttt tatggcggat atgaacaggc agaaagaaaa agatgtttga ttttccccga
    31741 ttattacgaa cctgtagagg aagattttga aacagcattg tatgaaatcc attatcctag
    31801 caaatttgcc agcctaagtc atgggaaaat ccttggaact ttagtgggaa cggggatcaa
    31861 acgctcttac tttggcgata tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa
    31921 tgaggtggat cattttgttg ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt
    31981 ggaaccaatc aaatacacag aaatgattat gccgaaagat agctggacac aagaaagagg
    32041 aacagttagt tccatgcgac tagacagtgt gatctctgag atttataata tttccagaca
    32101 gcggtccaag cagctgatcg aagctggtaa agtaaaagtg aactggacag aaaatacaag
    32161 acctgatttt gtacttgaac tattagatat tatttcagtc agaggttatg gcagagtcca
    32221 gatccaagaa cttgagggga agacgaaaaa agaaaagtac agggtactgt tcggtgtatt
    32281 acgtaaataa aataaaaagg tggtttactc atggctttaa ctccattaga tatccaaaat
    32341 aagacattcc cgacaaaaat gcggggatat aatcaagacg aagtagatga tttcttagat
    32401 ttagttgttc gtgactatga ggaattgact caaagaaatc gagaattaga aaaagcagtc
    32461 aaacattctg aagaaaaact tgagtacttc aatgaattaa aagatgcttt gaaccaatca
    32521 atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa
    32581 gtgatcgtta cttctgcaca aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt
    32641 gcacatcaat taacaactga tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca
    32701 gaaaaagcaa gacagcttgc gacagaaact gaagatctta agaaaaaaac acgagtattc
    32761 catcaacgta tcagcttgat gcttgagtct caattagaac aagtgaaaag tccggaatgg
    32821 gatgaaattt tacaaccatt ctccagctac gtgactgaca gccatgaagt gatcaaagaa
    32881 gttttagcaa aacagcttga caatgaaaat gatactgatg taaactcaga cacaggagtc
    32941 atagaagcac ctatgacggc atttgataca caggctattg atatctcgat cgtgcctggt
    33001 gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt cttaaagcga gccgatgaga
    33061 gtggaagttc ggtaagacac tgcatggtga aagatcctct acttgagacc tgcttttccg
    33121 aaattagagt aagaagcagc ggctgacctc gttattggtc acagagagtt tgcactagat
    33181 agatgcaact aaatttgggt ggtaccacgg catgttcgtc ccttgcggct gaacatgctt
    33241 ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg
    33301 caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg
    33361 caaaaagaat gggaagaaaa tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa
    33421 cctagctttg ttcttcatga tggacctcca tatgccaatg gtaatatcca tttaggacat
    33481 tcattgaata aaatcagtaa agacattatc attcgttcaa aatcaatgtc tggctatcgt
    33541 gcaccatatg ttccaggatg ggatacacat ggattgccga ttgaacaagt attggctaat
    33601 aaaggcgtga aacgtaaaga aatgtccatg gcagaatact tggaaaaatg tcgtgaatat
    33661 gcactttccc aagtagataa gcaacgagca gatttcaaac gcctaggagt tgcaggagat
    33721 tgggaacatc cttatgttac attagatcct tcttatgaag ctgcacaagt acgcgtattt
    33781 ggtaaaatgg ctgaaaaagg ctatatttac aaaggattga aaccaattta ctggtctccg
    33841 tcaagtgagt cttctttagc ggaagctgaa attgaatata aagatgtaaa atctccatct
    33901 atttttgtag ctttcaatgt caaagacggc aaagatattc ttgatgaaga tacagctttt
    33961 gtcatttgga cgacaacacc atggacattg ccagcaaacc aaggtattgc tgtaaatcca
    34021 aaatttacgt atgtattggt ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg
    34081 ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa cagttctaaa agaatttgct
    34141 ggacaagaaa tggaatatat gactgcaagt catccattct atgaacgtga atcacttgtg
    34201 attttaggcg atcatgttac tttagacgct ggtactggat tagtccatac tgctccagga
    34261 catggggaag atgactatat cgctggtagc aaatataaac tatctgtagt gtcacctgta
    34321 gactcaaaag gattgtttac agaagaagca cctggatttg aaggtatctt ttatgacaaa
    34381 gcaaatccta tgatcactga tctattaaaa gaaaaaggag ctttgctgaa gctagatttc
    34441 tttactcata gttatcctca cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact
    34501 ccacaatggt ttgcatctat cgataaattc cgtcagaata ttttggatga agtagaaaaa
    34561 gtagagtggg ttatcccatg gggtaaaaca cgtttgtata atatgatccg tgaccgcggc
    34621 gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa
    34681 aatggagaag caatcatcac accagaaaca atcgaacatg tggcaaaatt atttgctgaa
    34741 catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc
    34801 cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag atatcatgga tgtctggttt
    34861 gattctggtt cttctcatga agctgtttta cgtcagcgac cagaattatc attcccagca
    34921 gatatgtatt tagaaggatc agaccaatat cgtggctggt tcaactcaag tatcacgact
    34981 agtgtggcta tcaatggtgt agcaccatac aaatcagtta tttctcaagg atttgcttta
    35041 gatggtgaag gccgtaagat gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg
    35101 atcaaacaat ttggtgcaga tatccttcgc ttatgggtat ctagtgtaga ttatgaagca
    35161 gatgttcgtg tttctatgga tatcttaagt caagtggcag aagtatatcg taaaatcaga
    35221 aatacgatgc gtttcttatt agcgaataca gaagatttcg atcctaaaaa agatactgtt
    35281 tcgtacaatg atttacgttc tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc
    35341 aaagaaatcc gagaagaagg gtacgacaaa tataacttca tgcatattta ccgtacagtt
    35401 atgaacttct tgacggttga tctatcgtca ttctacctgg attttgctaa agatgtagtt
    35461 tatattgaag cagaagatga ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca
    35521 gctgtagctt tgacaaaatt attgactcct atcattccgc atacagcaga agaaatctgg
    35581 tcattcctga aagaagaaga ggagtacgtt cagttaagcg agtttcctgg ttatgaagag
    35641 tttgcaaacc aagaagaatt gatggatacc tggactgctt tcatggactt ccgtgataat
    35701 gtcttaaaag cattggaaga agcaagaaac agcaaattga ttggtaaatc tttggaagca
    35761 aaactaactg tttatccaaa acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat
    35821 attgcacaat tattgatcgt ttcaccagac tactttgaag ttatggcagc agatgaacaa
    35881 gtgccagata acgctatggt atttgatgat gttgctatta cagtggaaaa agcagacggg
    35941 gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag gagtagatga aaaacttcct
    36001 catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa
    36061 ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat gttctttgtc acaccccctt
    36121 ttgttatcaa tcgtttaatt ttccacgttc acgataccag ttatctggtt gccagatcca
    36181 ttcgaatccg tctttttcaa gtagttcgaa agctgcttta ggtcccattg atccagcttt
    36241 gtagttggga aaatccggtt gtgtttggtc ccaagctttt cgaatgacat ccacgatatg
    36301 ccaagattga gcgacttctt cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc
    36361 taatagtaat ttttcgtaag cttctgggct attttcaacc atttctgcac tatttctaaa
    36421 ttccaatttg attggttccg tttcaaatcc ttgccctact tctttcccat tcaaggataa
    36481 agaaaatcct tcagttggct gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc
    36541 gtcacatggt tcatcgacag atgttttgaa tacattaaca ggaacttgtt tgaatacgat
    36601 gttgatccga gtcccttttt cagtcaaacg tttgcctgta cgtacataga aaggaacacc
    36661 tgaccaacgg aagttatcga tagtgaattt gcctgcgacg aatgtttcag tctctgattg
    36721 agcatccact ttgtcttctt cgcgatagcc cttgaattgt acaccatcca attcaccttg
    36781 agcatattgc ccgcgaacga agttttctaa cacttcttct tcgttataaa tgcggatagc
    36841 tttcaatgct ttgatctttt ctgtacggat ttctttttcg gaaaatgcta ctggcggttc
    36901 cattgcaagt aatgctacaa cctgtaaaat atgattttgc accatgtctt taagtgcgcc
    36961 actatgatcg tagtagcccc cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg
    37021 gatattgtcg atatagcgat tgttccattg agcctcaaaa atattattgg caaaacggat
    37081 agctgagatg ttttggatca tttcttttcc taaataatga tcaatcctga aaatatcttg
    37141 ttctgggaat acttctctga tttgctggtt caattcaaaa gcagactggt aatcagaacc
    37201 gaacggtttt tctatgatca aacgattgaa gccatttttt gtcataatat tttgtgattt
    37261 caaatgagaa acgatcgttc caaaaaattg aggtgccata gccaaataat agacacggtt
    37321 accgtttaat tgatagtgtc catctaatcg gtcagacaat tcttttaatg tctgataatg
    37381 cgttgaatca tttacatcat gggattgata gtaaaaatgg cttgcaaagg tagttgcttc
    37441 ttcatctgaa ggattcaatg cacggattgt ttctttgaca acttcacggt agtgctcatc
    37501 gctccaggga cggcgagcgg ttccgattac agcaaaatgc tcgcctaaat ttccttttct
    37561 gtaaaggcgg aacaaagacg ggtacaattt acgctgggct aggtcacctg taccgccgaa
    37621 tatcgtaaac aatacatttt tttcattaga catgtggata cactccttta tattttatcg
    37681 acaaaaatcg tgctggccgc cttaaagctg acggggattt tctttctatc attttggata
    37741 gtgatcggtc cttcataagc tgcgatgtca acgatctcat attcttcgtt gatattcagg
    37801 tcaatagtga ctaagtaatc caacaattct ttttcatcaa gcactcgtgc gatacgcact
    37861 tttgtaccga caggatagga ctccaatgtt tgtcggcgtt cttcatggac aacttcctct
    37921 tttttaggaa tcgatcctcc atgaggacaa tgttcaggat gattcaaata gttttctaaa
    37981 tgatctgcaa gatgttccga agtcacatgt tccaatactt cagcatcatc atggacttca
    38041 ttccagctat agtctaaatg ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc
    38101 agactgctag ctttttgcag tccgcttgca gtcaattgta caccttgata aggggaatgt
    38161 tctacaagct cttctttcac aagtttagaa atcatttcac tgacagatgc agctgagaca
    38221 tctagactag atacgatttg tttattgttg actttcgttg tatctccacc tagttctaaa
    38281 atgattttca aataatcttc tcgatttggt gtcatattcg atcatccttt catggtaaaa
    38341 aaccatcatt agtaagtgta acaaaaaaaa cgttaattga ctatttttct aaataaaaaa
    38401 caaaggattt ttagacgttt ttacaacaaa aaacgaaaga tgatagagaa gactcattct
    38461 ttcgtttata aaggatatat atatacattg ggagggatct atttttcaga taacaactta
    38521 tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg gataagaaaa gataagataa
    38581 aaaccatact gatcacgtac gactaaacga tattcatcta tttctaccac agatgtgcca
    38641 agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc
    38701 tggctatcac taaagaacca tgtaccttga tactttgaga gtccttctga cttttccgct
    38761 tgttttttct ttttcaagta aataaaactt gagagaacgg taatgagtga accaagaaaa
    38821 tagatcattt tttttatcac caaatcactc ctttatgaga aaataatcgc atagacatat
    38881 tctttcttct ttatttcatt gtaccgaata aaagctaaaa tttctctaag actttcgact
    38941 ggttttttta ctgtttttct acaaattatg ataggataca tagtaggaag aaaggtgaca
    39001 taatttgaag tatcagcaag tggaaatagc tttttttatt gaccgcccga atcggtttat
    39061 agcacattgt atgaaaaaag acggagaaat aattattgcg catgtcaaaa atacaggtag
    39121 aggaaaagag gtcctgatac caggcgctga agtggctatt gtatttgctc ctggacctaa
    39181 acggaaaacc gcctatgatc tgattgctgt aaaaaagaag ggtgactggt ttaatatcga
    39241 tagtcaattg cctaatcggc tggcaattga tggtatttta gacggcacga tccttttacc
    39301 aggattaact ggtccattta cggtatacaa gcgtgaagta acttatcttc actccaaatt
    39361 tgatatttat ttagagacgg tggtcggaca aaaagcattt gttgaagtca aaggtatgac
    39421 acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc acactcagag gattgaaaca
    39481 tataggagaa ttaacgtatg ccgcacaaga tggttatgcc gcttatgtct tgtttattgc
    39541 tcagttcgaa catcttcatc tggcaacgat ccatgaagaa atgcaaccag cactagctga
    39601 tatggttcga catgcgcagc aaagcggagt tcaaatcctc gcctataatt gtcaagtatc
    39661 ggcagatgag gtaactgtca agcaggccat tccatttgat gtagatatcc cttttgaaga
    39721 tcctatacaa tagaaagaag gtaaaataat gatcaactta ggtattatcg gaacaaattg
    39781 gattactcat caatttgtcc aagcagcttt aagcacaaaa aaatatgaat tgacagcggt
    39841 atattctaga catttggaaa aagcacagga atttggttct cattatgaag gggatatcga
    39901 attcgcaaca gatttgaaga cttttttcaa tattactcat atggacacca tttatattgc
    39961 ttctccgaat agtcttcact ttagtcaagc taagcaagca attttggcag gaaaaaatgt
    40021 gatagttgaa aagccagctt tttcgacgcc aaaagaaatg gatgaaatca tccatttggc
    40081 aaatgaacac aatgtgtatt tttttgaagc agcaagaaat atccatgaat caggttttaa
    40141 aaagattgcc gattacttac cattaaaaga cgagataatc ggtgcaaatt tcagctatat
    40201 gaaatattct tcgcgctatg accaagtatt agatggagaa gaaccgaata tcttctcgac
    40261 tcatttctca ggtggtgcat tgatggattt aggtgtttac cttgtatatg cagctgttgc
    40321 ttggtttgga atgccaaaag aagtccatta tttcccaaga aagatttcta ctggtgtaga
    40381 tggtcttgga tgggggatct tacgttatga gaattttgat gtatcgatcc agccaggtaa
    40441 aatcggtgat tcttatttac cgtcagagat ttattttgac tcaggaacgc tagtgatgaa
    40501 tggagtcaat gcaattgaac aagcacaatt tcatgatcgc gcgcataaag aaatcattga
    40561 tttggcggta acagctgcag aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt
    40621 catggctcat ccgaaagatc cagcttgggg attgttgtat gaagagtgga tcgagctagc
    40681 tagaaatgtg aaccaagtga tttatgattt aagaaaaaat ggcggcatca aattcgatgc
    40741 agataatgag gaagattttt aatgaacgaa tttgtacaag tatttcccga aaattggcag
    40801 aaaaaatatg aggataaagg gtttatctca ccttctctta ttcaacgagc agtttttcag
    40861 ccactaagta ataaacaaag tatagtagcg gtttctccta caggttcagg taaaacactg
    40921 gcttatcttt ggcctctact actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt
    40981 tttgcttctt ctcaagaact agctattcaa gtagcagatg ttgcacgtga atggggaaaa
    41041 gacaaagagt tgaaagtcca atctttggtg ggcggagcca acgtcaaacg tcagattgag
    41101 ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag gaagaatatt ggaattgatg
    41161 aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg tatttgatga agcagaccag
    41221 ctatttgacg ctgggaacag ccaaatcatt gatcaaattc ttcatcaagc tccaacagag
    41281 tatcagctag cttttttttc agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc
    41341 actgggaaaa cgtttgagac aattgacgtg acagcagagg acgattctcg aaaaggactg
    41401 agacattact ttttacgtgt accgatacga aagaaagatg actacttgag acgtctcacg
    41461 catatagaag attttcatgg tctgattttc tttaatcaac tgactgaatt aggcgtaatg
    41521 gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc tggcctctga tcagaacaag
    41581 ctactccgaa aagcagcgct agaacaattc aaaaagaaaa tggtcagagc acttttgacc
    41641 acagatgtgg cggctagagg tttagatatt acaggtcttc cttatgtagt gaatgcagaa
    41701 gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa gaactggtcg aatgggtaat
    41761 gaaggaatag taattacact cgtacaagat aataatctaa gagatttaaa acggctttta
    41821 cgtccatcaa atataacact tgaagagatt tttttatacg gaggtaagct gcaaactgag
    41881 cagccaacca aagaagatgc agctcctgca gtaaataaaa agtatgcgaa aactttttct
    41941 ggaaaggagc agaacccaga atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa
    42001 tcaaaaaaag agagaaataa aggagcaagg cgcaaataag cgccttttta tgctatgatt
    42061 atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag
    42121 agatcttgca gctattgaca gaaaaccaac aaggattgac agcaagtgat gtagctgagc
    42181 gcttaactat cgatcgaagc aatgcaagcc gatatttaag tgaactatac aaggcccatc
    42241 atattgtaaa aaccgctggc cgaccagtcg tttactcttt gccaacggaa aagtcaaaat
    42301 ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt tgaaacactc gttggagaaa
    42361 atgattcttt aaaagtcagc atacagcagg caaaagctgc gattctttat ccaccgcggg
    42421 ggttgcatac gattattttt ggagaaactg gtacagggaa gtcgatgttt gctgaatgta
    42481 tgtatcattt tgcaatcgat tcagagatgc tttctgcaga tgcgccattt gtttctttca
    42541 attgtgcgga ttatgctcaa aatccgcaac tgctctttgg acacatcttt gggatcaaaa
    42601 aaggggctta cacaggagca gctcaagata gtcccggtct aatagccaaa gcagacggcg
    42661 gtatattgtt tttagatgag atccaccgtt taccgccgga agggcaggaa atgctgttta
    42721 ctttcattga taaaggtatt taccgtccgc ttggagaaag tgcacaagta catgaagcat
    42781 ctgttcagat cattggggcg acgactgaat cttcagaaag tttcttatct acgttcaatc
    42841 gacggattcc tatggcaatc accttgccga atcttgcttc acgttcactg gatgagcgtt
    42901 atgaaatcat ctctcttttt atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata
    42961 tcgaaaaaga agcgattctt gccttcatgc tttatgatgc ggaaggaaat atcggtcaag
    43021 tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt acattaccgt actcatcatg
    43081 agaaaaagct aatcattcga aaagaagaac tttcccttca agtacaaaaa ggattactga
    43141 aaatcaagga aataccggaa cgtctcgacc gattcatgga cagcaaaagt cagtatttga
    43201 catttgaacc aggatttgct gatgttgtct ggtcacaaga tccagaacga aacatgcagg
    43261 tctataacga tattgaggaa aaagtcactt ctctttccga aaccggtgtg gagaacattg
    43321 atctagagac cctcatttca aaagatgtgg atgcttattt ccagacatat gtcaaagaat
    43381 tgactaaaag ttcgattccc aaagatcttt tgccagatga aatctggcgg ttaaccaatc
    43441 aattgtatga tgtggctgaa aaacggttag accgtattta taatgaaaaa gctcgttttg
    43501 cttttgcttt gcatctgcaa agtacattgg atcgtgtgag agaaggtcat atgatcgtcc
    43561 acccagattt gaataacgta cggaaaaatt ttaagaggga attccaggta gcaatcgatc
    43621 tttcaacgat cattgaagaa gaagaacaag ttgagattcc ttttgatgaa atcggcttta
    43681 ttagtatgtt tctttctatc aatctaggag aaagtgagcc tgttaaagaa aacaaagtag
    43741 atgttctcat attgatgcac ggcagacaga ctgcttcaag catgttggag atggcacaag
    43801 aactattagg aacaaaagta gggaaagctt ttaatatgcc actggttatg gaagttcaaa
    43861 caatgtatga agaagtgtta caatatgtca aaaactatca agagcatttg acgaatggcc
    43921 tactcttgtt aacagatatg ggatcattaa acaccttcag tgatttgatt tatgaagaga
    43981 ctggcattcg gacgaaagct atcacgatga caagtaccat gattgtgata gaagcgatac
    44041 ggatggcaag tgttggaaga agcttagaag atatttatca aaatatccag ctttcctttg
    44101 aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag
    44161 ccgtaatcgt gacatgcttt actggtgaag gagtagctgc caaattatat cagcgtatta
    44221 tgccagtgat taatcaaaat aaggtagaac tgatccagat gcagttttta gaacgtgaat
    44281 cattcaaaaa acacatcgat gccttattgg aagaatatga gatcaaagca attgcgggaa
    44341 cagttgatgt tgattatcaa aatatcccat ttttttcagc ctatgacgtt tttgatgatg
    44401 aaaaactaaa tattttaaag cgtatagctg atgatgagat cccgacagct aaaattgtcg
    44461 attcacttgc tggatcatta agcgatgtct tttctattga tgaactgatc cgttcattgc
    44521 aaaagacagt tcagcagatc cagaccgatt tgtcgattat cgtggaacct ggagtagata
    44581 ctggaatcgt tatccattta gccttcatgt tagacggatt gatcaaacaa gaaagccatc
    44641 ggcaatttga gaaactgtct attttctcaa aaatttatcg tttggaaatg gatatcgttc
    44701 gggcaaactt gatggcacta gaacgaaaat atcaggtaaa aattccagaa gatgatatcg
    44761 catttcttac acaaatgttt cttgaaaata aaataaaaaa atgaacacac taatcatact
    44821 gtttacacac taaacatctg tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc
    44881 tatttcagct ctttttttta ttggcatgct tcttgcgtta tataaaagtg taaaaaaata
    44941 aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag tttgttcagc agggatgagt
    45001 acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat
    45061 atttttgcgg tctctgcctc agatgcagat aataatttag aatctaagaa tgtcgatgta
    45121 ttattattag gtcctcaagt acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct
    45181 aaaggtattc cattagatgt catcaatatg caagactatg gcatgatgaa cggagaaaaa
    45241 gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt atgcagttgg gggtataaca
    45301 acgtggaaga tcaacaaaat ctagaagcca tcatggggtt aatcatgttt ggcggtaatg
    45361 caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg
    45421 ataggaaaat cgctgatgca gaagaatcac ttatacaagc gcatcatgca caaacagaca
    45481 tgttgacaca agaagcacaa ggaaatcata tgcaagtgac attattgact gttcacagtc
    45541 aagatcactt aatgacatct attgcgttta ctgatttagc aaaagaaatc atcgaacttt
    45601 atcgtcgcgt atacagcgaa taaatacaac ttcttttttg aacggaagaa gaaaaatttt
    45661 tgaacggatt ttttattttg ttataaggag aactctcttt atataaaaaa cttataggag
    45721 gaagtaaaaa atgaacggac tatcaagttg gatggagaaa tacatcctgc cggttgccgc
    45781 aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat gcttttatcg gcacactgcc
    45841 cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca attattcgtg atttaccgca
    45901 acagtttaac agtacctatg ctgacagttt agcagcagac aaaggaatct ttgccgtatt
    45961 gaatgtcatc atcggaatca atggttttgt ctggaacggt acactagcaa tcgccggatt
    46021 gatctttgcc ttttcttggg ggtataatct tgcccgagct tatggagtaa atgatctagc
    46081 tggggggatc gttggtttag ctaccttgat tcaaggtatt tcctttggtc cagcattatc
    46141 agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat gcaatcaata gttctgagat
    46201 tggtgcaacg attgcagatg gcacactgtc agttggatta tggggttggc taaaactaga
    46261 tcatttgaat ggaaacgctt attttacagt tatgatcatg ggagctattt cagtcattat
    46321 tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg cctgattcag taccaccagc
    46381 agtatctaaa gcatttgcag ccattttacc agcgacgatc gctttatacg tcattgctat
    46441 tatcaattat atcgtaggtc gtgtagctga tgggcaactg atcattgatt tggtgcaaaa
    46501 atatattgca gaaccattcc taggattatc tcaagggttg ggagcagtat tgatcgtcac
    46561 agtatttgtt caagtattct ggttctttgg tattcatgga tcaaatgtat tggcaccagt
    46621 gctagaggga atctggggac aagcacaact aatcaatatt gatattttcc aaaaaggtta
    46681 taacggaaaa actggtacac cagccgtgtt agatgcaatc gaagatggaa aagcttatat
    46741 gtgggtccgc ggatcgttcg atgcttttgc atggtttggc ggatcaggcg gaacgatcgt
    46801 cttactgata gctatcttga ttttctctaa acgtgcagat tatctaacag tagctaaatt
    46861 atccattggg cctggtatct tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt
    46921 attgaatgca atcatgttta tcccattctt gattgctcca gtagtagcaa cagcaattgg
    46981 atggacagct acttatcttg gcctagtcgc tccggtatcg cagcaagtca catgggtagt
    47041 accgccgttc ttattgtcat tcttagcaac aggagcggat tggcgggcac cgatcgtaac
    47101 attagtatgt atggtcgtga ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa
    47161 aatggaccct gccgcaggcg aacaacaata agatgaacgt gaggagcggg acgctttaag
    47221 tttcgttcct cataatgaat tttcgaggag gaactgaaat gattacaatt gccagtgcaa
    47281 tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa tgaagaagga atgactttta
    47341 catttaaaga aaaacaagga atcaagctgt tctttgaaac aagtgcagaa gatccggaag
    47401 ttgcggctaa aaaagcaaag gaactaatca aagcacagcc ttggggaaca gtcctttatt
    47461 ttcaatcaac agctgtttag aaaatggttt aaaaagatag gatggttgag atgataaaaa
    47521 acggtttatt tttatgtagt attggttctt tgatcgtgtt gtatagtttg aatccacatt
    47581 cgcaaaggta tgatttgtat agtatggtca caggtctttt tctaacagga ttaggaatct
    47641 atttcttcat gaaggggaaa aaaagagaag aagcaaagga gaaaaataac taatggaaat
    47701 cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat aaagttatgg actctgtcct
    47761 tttcgatgta cgtaaagcga ccggcaaatc agtaactaga aaacagctga aaaatttgga
    47821 gtacgtcaaa caattctcgc aaaataaccg ggctcgcata aaaatcgaag aagtcattga
    47881 aaaccagtct tataagtttc gtacatcgac aacgaagaat gaattcgtcg ttcattatca
    47941 aatcaaacca ttggacgatt cgacctgtga ggttcattac acagaaaaaa tggagtctta
    48001 tggcatgctt caaaagttga atgatatgct tttaggaaca atcttgatgt attttaaacg
    48061 gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg tattaaatca atgaaacatg
    48121 gtactattta gttattgaaa aatgaagtaa gctagaagga atttttcata ggaagataga
    48181 tctttcctgc gaatcctcac tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa
    48241 gtaagagagc taggtgtatc ttcgattgag gaaatgtttg atttagccac ttatgcattt
    48301 aatggggcag atacacctga aaggcgagaa cgttttcgta cacttgcgga gaattcttgg
    48361 aattacggat tttttgatga ggaggatcgg ctaacaagtc aagtcatggc gacccctttt
    48421 ccagtgaatt tttacggaca agagtaccta atggccggca ttggctatgt tgcttcttat
    48481 ccagaagcaa ggggacaagg cgggatcaat cagatcatgg aaaagatcct tgaagattgc
    48541 cgtgatcgta aagtaagctt atcttatctg gcgccattct cctatccctt ctatcggcgc
    48601 tatggctatg aacaatcatt tgataaaatc agttatcaac taaacagtcg tgatgtgccg
    48661 tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt ttgaagatgc aaaagctgca
    48721 atcagaagaa tttatgaaca gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac
    48781 tggtggtata cgtataaatt caagcaaaaa ggcaattacc agtttgcttt gcattttgat
    48841 gataatgagg aagcaacggg atatatcgtt tatcagcttg caacgtcttc ctttattatt
    48901 gcggagtggg gatttttgga tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct
    48961 cacaatggtg cttttgaaac atttgaatat tcatgcggct acggcggtaa tgatcaaaat
    49021 tatctattag aaaatccatt tgcttctgta atgatcacac cttatatgat ggcgaggatc
    49081 gtagatattt ctttgttttt ggaaaattat ccatttaaaa agagacaagc tgcatttgcc
    49141 ctacaaatag aagaagatca ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc
    49201 gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa aactcccaat cgttcgagga
    49261 agtatccaac atatcacgca gctattatta gggtatcgta aagtggaaga gttggtattc
    49321 caagaaagaa tacaggtgac aaaagaatat gaagaaacgt taagctcaat cttgccacag
    49381 caacagccaa ttcttaatga ctatttttga taaaaaacgg gtgatgacaa tatttttgtc
    49441 accacccttt tgtttttcat ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt
    49501 tcgtaaattt gaattttttg ttttcttttg ttttagggtt ttcttttttg tggtcttgga
    49561 ttgcttcttt cgtaatcttt tcccgatttt ttttagatga atctaagcgt tctgcgtata
    49621 ctttttcttt tggcttatca ttcattggaa aggacctccc tacattattt cattctacct
    49681 ctagtttatc atctgttaag gactagtgga aacgttttgc ctaacttata agctgatttt
    49741 tttagcagat aagcttgcaa gtaaaaaaca aacaagctac aattgattta ttgaaattgt
    49801 tcctgtagct cagtaggata gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc
    49861 cagtcaggaa cgtataaata acccctgtat cctttgcggt acaggggttt gttttgtatt
    49921 tgtgccaaaa atgtgccaaa ctagttatca tttttatata aactatcgaa cacagtattg
    49981 attttttctt tatcctgatc ttcaagctct cgaacaatat gaagataagt agacattgta
    50041 gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa
    50101 agtaaaatag atgcatgtgt atgccttagt ccatgcaaag taaattgctt ttcaatccct
    50161 aattctttta attttttgcg caaatatttg ctgacggcgt ttgatgaaac taatccattt
    50221 tttaagttga aaaaaacaaa attatgaggg tttttgattt caaaattctc gaataattct
    50281 ttttgattga ttttaaactt ttttaagaga tcaatcgtat gtttatcaat ggaaattttc
    50341 ctatttgaag tttcgttttt tgtttttccc cattcattta atttataatt ccatgtttta
    50401 ttaatagtga tgatttgatc ttcgaaatcg atatcttccc atgttaatcc tagaagttca
    50461 gcaaatcgca ttccagtggc accagcaact aacacgagca taggagaaga gtactgcgca
    50521 gaaaggtttt cttctacgag tttcattaaa gatttaaatt gatcataatc taaatattta
    50581 tcttcttttg gcttcaatga atcttttcct ttgattactg cttttctagt tggatcaaac
    50641 ggaattaatc cttcttctac ggcatctttc aacgacgctc taatatggtt attaaactta
    50701 ataacggtag attttacatg gtctttagca tacttattta aaaagcgttg atatcccact
    50761 ctatctaaat cagaaatcaa taccgctggc atatatttct ttatgttcat taacgtatct
    50821 tcgtattttc tataagtgat aggagatact gttccttctt tataaagttg catccaatcc
    50881 tcaaagtaat ctgataataa tagatttttg cgatcccctt taaggccttt gttcaagtta
    50941 tattctaatt cgttggcggc atcttttgct tctccttttg tcttaaatcc tgattttctt
    51001 atcttcgtgt attttccgtc atcttttttg tatgaaattt cgtattgcca actatttcca
    51061 cgtttgacta atcttgccat aattgaatca gctctctttc tctgatacaa taggaactat
    51121 aaagaagccc attgtttggg ttttattatt ttttgcacat cctttttgct tgccggcgca
    51181 aggatgtgct atttgtcgtt ttaatggtat aattggatgt gtattagatc atccaatcag
    51241 aggtgtttat tatgattaaa gtgattttta aaagaataac tgaacttcca gagtttagat
    51301 tatttgttga ttattggtat gtctggtttt ttattgttgc attgctgata ggtctagatg
    51361 tcatattaga aagaaaactt aagtaagagt tgagttaata aattgaattc ctaaatgaga
    51421 tattttgtag cccatatttt gtttgccgac atcacctaat actatggagc taatcgtttt
    51481 ttctaacaga ccaaaatcag ataatctatt taagctacca tctattagct cttgtgaata
    51541 gccagacatg ctgatatttc cagtgaaatt ttcgtttcca cgactcatta agcatgctaa
    51601 caattgaatc tcaatcgatg ttaattgatt cagtacaaga agataatact cctcgttatc
    51661 ccatgaagcg ttgttaattc gaagtaaaca attcttataa agatttttat acatatctat
    51721 tttattttgt gcctttgatt tttctacctc gtcatttatc ccttcgaata ttccaatgag
    51781 ttggtccttg ttctctaagt tgaaaacatc ttctggaatt tgatcgcgaa tgttttctaa
    51841 tgattcgttt aaatttttgt agaatttctc gatgcgtttg aaacgcttct cgtttttaga
    51901 cccaaaataa agtgactgca caacgccacc aatataagga attgctccaa taccgccttc
    51961 aactattaat tcaagatatt cttttttggt tagtttttca ttgctattat ccatagttac
    52021 ctcctatttt gtcatttcct ttacaagcat tgtgactcct gatggcactc ttttgtatag
    52081 gttttattat tgtgcacaat ctaactctta gtggggcgtg gttcatttat tttttactta
    52141 ctttactatt aatcatatcc cattgagcat aggatttttc tatgttcgat cctcttgaac
    52201 taatttctcc tgtaaaagtt actttatcgc cctttgtaaa ggtatcagca ttgtttaagt
    52261 ccttcgcaaa aattacatat gaaactttag gtgttgcaga aatatcattc caacttttat
    52321 catcgtactc tttgtctgct ataatggcta ttctagtagt cattgattcg ataacagttc
    52381 cttcaaaagt atatgtttta ccactaatat atttagtaaa agctgtattt tgctcagtag
    52441 ctggagtaat agaataataa ctatctaaga aagcacccca gtcatgatta tttgtctcta
    52501 aaaattcttc aaattgagtt aggcttttat tgctgctgtt agatgtagta gtttgagtat
    52561 cagatgatga gctttcctgt ttgttattgt catttgcctc gtttgatgag gaaagcgaga
    52621 aacctacggt agtaaaaata atcccgataa taaggagaat cataggtgtt ttcttatttg
    52681 tttttttgat aaatcttaca attaacatga ttaaacctat aataaataaa acaataccag
    52741 taaaaccaaa tataccgtcc attaatagat cctctttctc ttttgatatt gaatattata
    52801 aaccaagcaa ttcttttttc ttaaaatcga attcttcttg attaataatc ccttcatcta
    52861 acaactcttt atacttttta atttcatctg cacttgaaat ttgtgtttct ttggaattat
    52921 tatctaatat agattgttgc ttctctatat atttttttaa attttccatt tcattccaat
    52981 atttttttgt aaacataacg gtgttttcat ctttcgtggc cgctaatata cctccgcgac
    53041 tttcgtttcc gcctagcaag gtaaattgta tataaccatt actcatacct ggttttttta
    53101 attgtacggc tgatatattt ttaaagggga tagttttttc tcctttaata ccttgattaa
    53161 ctaaatttat aaatcctttt ctaataattg aaatagaatc atcagttact ttaatcaagg
    53221 tttttcctgg agattttatt aaaatctctt tttccatttt ttcttcctca tttctgtgat
    53281 atgatttttt ttgtagaagc tcatatatga ggaaagagcc cgtgttgcag cacgggcttt
    53341 tttaatgttt tgctgaaatc ggttttttta ataattcttg atgtattcat acatattgcc
    53401 ttgcgtaaga atattttttc ttaaaatagc attggcagac agcataacaa gtgtatctga
    53461 gcttattctg aatagaataa tactccataa atttttcgag attaaactga gattcatcag
    53521 tcagttcgtt ctcaatatag atatttaata aaattagaat agctatttta tctgcttctg
    53581 tttcaaattt tgagtgaaaa gttgtagaag tatcgtacaa aactgaaaat tcaaaatgag
    53641 aggcgctgaa atgtgcaagt tcatgagata aatgaaaggc ttctgcagtt tcaccgtata
    53701 gattttcatt caaaaaaatg attctgggtt ttggatagta gaaaccaggc tctttcatct
    53761 ccatataaac tactttcaaa ttgtattcgc tcaacatttc tttcaatttc aaatacatac
    53821 aaaccatcac tccaactatt cattttcctc taaagcttta gcaattgcaa tcgctttacg
    53881 cattgtctcc ttagatattt cttttccatc aaaagaaaaa acagtatcgt cttctgataa
    53941 atccacatgt ttaggggttt ctctttcttc tctacctaga aggtagtcta cagagacatc
    54001 gaaatagtca gcaatccgac ttaattcatc tgaattagga gtattatttt tccactttgc
    54061 tagataaccg ttagaatatc caaaattaat ttctaactgc cgtatagata tctttttcct
    54121 cttcgccaag tcttttatta tttcataggt attcatcgat aaaacaacct ttctaaatgt
    54181 ttacaaaaaa agtttagaaa aatacgcaga aataatttga ctaattctga gtaacgagct
    54241 atactatatc ttgtaaacag atttaacaac taaaaagaca acaaaaaaca ctattgatat
    54301 gtaaatgcag accgccaaga aagctttaaa atcaatgttt ttatgtctta tttaattatg
    54361 ctcttagtat agagtattac tcagatactg tcaattgaat ttagaaaaaa gttgttaaat
    54421 ttgtttacga atataaaaga aaggaatgag aaatatgaac acaccgcaaa tttttaattt
    54481 cgaacaaaac gaagttcgga caattttagt aaatgatgaa ccatattttg taggcaaaga
    54541 cgttgcgagt gttttgggtt attcaaacac taaagatgct ttgtcgcggc atgtagattt
    54601 ggaagataag atggggtcgc gaatcacgac ctcaggtcaa tcaagagaga tgacaatcat
    54661 caacgaatct ggtttgtaca gtttaatctt aaaatcaaaa cttccctctg ccaaaaaatt
    54721 taaacgttgg gtaacaagtg aagtgttgcc agcaattaga aaacatggag gttatctaac
    54781 tccagaaaaa gtagaagaag ctttgcttaa tccagataca atcattcaat tagcaactca
    54841 actaaaagaa gaaagaactg gaagattaat cgcagaacaa aagattgcag agtacgaacc
    54901 taaaatctcc tatttagata gcatattatc ttctacagat tcagtaacaa ttagtcagat
    54961 tgcagcagat tatgggatgt ctccacaaca gatgaataaa ttacttcata aactaggtat
    55021 tcagaaaaaa gtcggtaacc aatggttatt gtgcaaaaaa cacatgaacc aaggatacac
    55081 aaaatctcat acaactgaga tcccgaaagc cgatggtggc actaaaattg taatgaatac
    55141 caaatggaca cagaaagggc gtctatttat ctacgaatta ctaaaaaaag aaggatatta
    55201 ccctcaaatg gatttagagg aaattggtta gaaaggagtt ttagtatgac tgacattgca
    55261 gaaatcactc aacgagatag agaaaaaatc aaagaatatg tcgaaagttc aaagttctta
    55321 acttacacca tgcttgctga aagatttgga attagcaaaa gctacttatc tttaatttta
    55381 aacggtaaaa agacttctgc agaagcaaac agaattatag attcgattat cactatgtac
    55441 gaattgtaaa gggaggaaca gctaatgcaa tatctagaag caaaaatccc aattccagaa
    55501 ggctatgtaa ttatctccca agtggattat gaggagttaa aaaaagctga tgatactggt
    55561 agatggatga cgttgccaga agtgctagaa cggattaaca gaaaatatga ttggtttact
    55621 tctagagttt taaagaaccc aagatataga aatattatcg atatcgagaa aaacaaaaat
    55681 ggatttgtct attatccagt tgaaggaaga gacacatatc tatttttaag aagtaaaaca
    55741 cttgaatttt tagaaacaaa tttttcggaa atcttaagga ggcaagcgga tgggaaaatt
    55801 taatagagcg ctagtgttca gcgcaccgct aatcatctac gctttaggac tttggggaag
    55861 caggcaagcg ttgataggaa cgattgttta catggtttgg atttttatgg ggcttgatga
    55921 agctgaagct gagtacagag cgaaaaagcc aaccgagggg gctgactaaa aatgaataga
    55981 aaagagaaac tagaatgtat attactatta ctcagtttaa ttctttcact aatttctcta
    56041 ttgggaagtt tgtatttttg atatcaacaa ttttgtggat ttgggttcta tttagagtaa
    56101 atatcatttt tgtttggtga ttaaggagtt tattaaaatt aattttccct acttcaaatg
    56161 caaggtaaca agatatagca cttttaggag gaatatttag cggtacagct tcagagtata
    56221 ttctattatc atcgactgat cctcctttag caattaattt cttaaatgga gtagctgacc
    56281 atctgcggtt taagttagcg gatgaaagtt ctaaattaac taacatagct ggttctgttg
    56341 aatagttaga aatgattact tttgtataaa tcatatctgc agcgaagtaa gaagcattaa
    56401 gttcaacttc tagttgaggg cgcattcctt ttatttttat ccctgttaaa aacgtactca
    56461 atataaaact tattattgac atccattgaa aaattgttaa gtgaaaaaaa ttcaaaataa
    56521 acaccaccag tttttaacta aattatacca aaaaggagag aagaaataat gcaagaatta
    56581 gtaattttga aaaataaaga agctgtgact acgagcttac aagtggcaga aagcttcgag
    56641 aaaaaacatc aacatgtttt aagagatatt gatgcactaa aaaaagatgt gtccaatttt
    56701 ggacagatgt ttgtagaagg taatgaacca gattcatatg gcagaaatcg acgagttttc
    56761 ttcattagta gagatggatt tttcttgctg gctatggggt ttacaggaaa gaaagctatc
    56821 tacttcaaac aaaaatacat tgaagcattc aacgaaatgg aagatgttat tcgcaagaat
    56881 actgttcctc aaacaattga agatatgatg atctatcaat tagaagaaat gaaagatgtt
    56941 aaaaaagatg tttccatgct taaagatact atgcgaatta gcggacaaca agagtttgaa
    57001 attaagcaaa aaggaaatat gaaagttatg ggagttctag ggggaaaaga aagccgagct
    57061 tatgaagaaa tcagcaaaaa agtattctca aaattttggt ctgaatttaa acgtaccttt
    57121 tcaatcccaa gatatggcga gttacctcgt aagagattcg atgatgctgt ttcatttatt
    57181 gaaatgtggt tgccagaaac tgcgatccgt atggaaattg atcaactgaa cagacaacag
    57241 agactttttg gtgatgacaa tgaatagagc tgaagcgcta agaataggga cggcaattgc
    57301 taattgctgg tggaaatact ataaaccaat catcctaagc caacaacata ttgacaagca
    57361 aaaagcatgg caacaaataa aaaagtgact ccgccggcaa gcaaagagtc acaaagaaaa
    57421 cacatcataa ggagatttta gcatatggaa aaagaacttt ccactctaga tcaatatttg
    57481 attgatcctg attggggcaa gccgaaaatt gaggaaacaa gtggtcgaaa aatcagacga
    57541 aatcttttga cgaatgaaga actagcttgg gatcaagatg atttaggcaa ccatgtaact
    57601 atttgggatc atgtttatct tatccatcta tcgaagcatt cgaataaacc tgaatatatt
    57661 tacgtcatcg aagatggctt gattgatgcg ctagaagagt acgacagaga taacttgatt
    57721 gatatctctt attacggacc aggtaagaaa tacattgctg aaatggaggc agaatttgat
    57781 gagtgaaatc aaagggacaa cgaactttga aaaacttttt agtcgtaagt taaataaaat
    57841 tctcaagaaa aaaggaaatt ttgattattt atcttgggct cacgcgtggg agattatgaa
    57901 aaagaatgat ccacaggcaa cggtaactat taatgagtac aaacactaca gggttgtttc
    57961 tggaactcat caagactttc ttgttgagga atataaacct tttcttatgg atgaaactgg
    58021 gacttatgta tctgtctcag taacggttaa aggacacacg gaaaccgaat tatttcctgt
    58081 tttagattat cgaaaccaac cagttgttaa accaaatgca atgcaaatca ataactcatt
    58141 gaagcgatgc tttgtgaaag cattggctct acacggactg ggattatatg tatttcaagg
    58201 ggaagatatt ccaacaccac ctagaatcga tacaaagaaa ttaaacatgc tagagacgat
    58261 tctagaagct ttcaatgagc agatgggtaa agatatgacc aaaaccttaa tcgaatatgt
    58321 taatgagcag acagataaat tagggctctt agctgataac gttgaaacta ttgaacagtt
    58381 aagctatgag caatgtgcct tgatggagcg agcaatagca gctaagagaa aagaattaga
    58441 taagaagtga tatgagtgtt taaaccatta atcgattcat attcagcggt tctgaaaaag
    58501 ttcaaaggaa aagacataag cgcaaccatc aatgaggaag tgaacattga tcgactaaag
    58561 acgatgtatg acggctacga tggtgatcga gtcattgaaa ttcgttttat tgatcctaga
    58621 cgtttcaccg tacagcaacg aaacttcatc tatgcgctga taggcgatat ttttatcgat
    58681 acaggcatgc caacggactt ctggaaggaa ttcttctact ttcgttttga aggtgtcaca
    58741 gggcgcaaaa taagcctgaa agacgaatcg aatacaactg tgagtgatgc caatgtctta
    58801 gcaaatatca tcttagattt catctttgaa catcatattc ctttcaaaga aggctatgag
    58861 attttacctg cgaatcaaga atattacttc tacaaatgca tcacaaaaag agtctgctgc
    58921 atctgtggca aaacaggagc tgacatcgat cactttgaca aagcgctagg aagacgaaag
    58981 cgcaaagaag ttgatcatgc agagtacaca tttgcagcac tctgcagaat ccatcacaca
    59041 gagaagcaca aaataggtgt gattaatttc aaaaataaat atcaaatcaa agggatcaaa
    59101 ttaaaccaga aaacaatcaa aaagttaaat attggagggt aaaaatgaca gaacatcgaa
    59161 gttattatgc gattatacca gccaacgtaa ggtacgacaa aagacttaaa ccaaatacta
    59221 agttgttata cggagagata acggccttgt gtaatgaaag aggcttttgt tgggcaggca
    59281 atgagtactt tgcagattta tatggtgtga ataaagagac catatcgcga tgggtaagtg
    59341 atttgattaa gtttggatac ttgaatcggg aaatcattta caaagagggt accaatcaaa
    59401 taatcaatag gtacctacga attaatcaat accctattga cgaaaaacgc aataccccta
    59461 ttgacgaaaa agtcaaagat aataatacat ctattaataa tacatttaat aatacaaaag
    59521 aatatataag agagttaccg ccttcgaaaa aatcgaaggc taagcccgtc cgtcataaat
    59581 acggagagta taaaaatgtt cttttgtcag atgagcagat ggagaaactc aaaacagaat
    59641 tccctaatga ttaccaagag cgaatcgaac gactgtcaga gtattgtgaa tcatctggta
    59701 agacttataa aaactatttg gcaactattc gaagttgggc aaggaaagaa aaaagtgaac
    59761 ctaagaacgc aagcagtgga tacaagcgca caggaagacg agagaagctt cctgaatggg
    59821 caatcgacca agaagcctat ctcaagaaaa aagcgctaga acgagctaat agacaatcaa
    59881 aagcaccatt ctaagaggtg gaaaaatgaa gatcgattat ctagaactaa ttaatgaaat
    59941 agcgaattat aaaaagggcg aggaattaga cgtcctgaga gacgtatatg atcaactcga
    60001 agaagctgga attgaaggaa ttaagaatga tcgttcgagt tggagtaagc tcagatacta
    60061 tttcgcactt tatatcgata caacacaatt aagaaattta gcatatacca aattactatt
    60121 tgttgattgt gttaaaggct tgcaaaaaca tcttaatgaa cttgagcagg tgtaatcaga
    60181 tggacctaaa gacatttaca gcacagattg aactaatgca tcaagaagct ttaagacaaa
    60241 gtgtatcgta cgaagacaag tggctcaaca cgttccatgg cggacgtgag agcgcacttg
    60301 atcaagtact caaattactg aaaggagaat gtcgggatgg ataagaaagc ggcaatgcag
    60361 cgaattatcg aattgactta ttcagaagat tggcaaaatg acaaagaagc tgcttcagaa
    60421 gtgatgaggc ttggaagagc gatgtgggca gacaagagca acaagccaag accacgaaaa
    60481 atcgcaattt ggcacggtga caaacttcta gtgataggga cagctgaaca gttagcaagt
    60541 ctcacaggct tgcacgagaa aatcgtgagg aaaagagcaa ggtgtggcta cacagacgct
    60601 aagaagagaa cgtttagata cgtggaggaa tcatcatgac aacagaagaa gtgattcaaa
    60661 tgcgtattcg aagccttcag cgtgagattg acgatctgga acgaacaaag gcagtgatgg
    60721 tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga gaatttaaga agggaaatcc
    60781 atcgattgga ggaatgagcg tggataagaa agcagcaatg aaaagaattg ctgaattaac
    60841 caagtcagaa tcttggcaag aagacaaaga aatagttgca gaagtccaaa agctcggtaa
    60901 atcaatgtgg actgaaaagc ccaaacggag aacgccgaga aaaattgcaa tctggcatga
    60961 tgaccgaatt ttagtaacag gtactgctga acagttatct gaaattactg gattaagcaa
    61021 aaacattatc tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag
    61081 gtatgtggag gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa
    61141 atcatgatga agattcagaa aga
//
LOCUS       DASHFF010000009        60145 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.009, whole genome shotgun sequence.
ACCESSION   DASHFF010000009 DASHFF010000000
VERSION     DASHFF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.009"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..591)
                     /locus_tag="U2I75_000668"
     CDS             complement(<1..591)
                     /locus_tag="U2I75_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1695824.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(616..795)
                     /locus_tag="U2I75_000669"
     CDS             complement(616..795)
                     /locus_tag="U2I75_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695825.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(872..1381)
                     /locus_tag="U2I75_000670"
     CDS             complement(872..1381)
                     /locus_tag="U2I75_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695826.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(1477..1856)
                     /locus_tag="U2I75_000671"
                     /pseudo
     CDS             complement(1477..1856)
                     /locus_tag="U2I75_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            2186..2935
                     /locus_tag="U2I75_000672"
     CDS             2186..2935
                     /locus_tag="U2I75_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN1695827.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(3119..4396)
                     /locus_tag="U2I75_000673"
     CDS             complement(3119..4396)
                     /locus_tag="U2I75_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695828.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4475..5119)
                     /gene="pcp"
                     /locus_tag="U2I75_000674"
     CDS             complement(4475..5119)
                     /gene="pcp"
                     /locus_tag="U2I75_000674"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN1695829.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(5318..6262)
                     /locus_tag="U2I75_000675"
     CDS             complement(5318..6262)
                     /locus_tag="U2I75_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN1695830.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(6264..6950)
                     /locus_tag="U2I75_000676"
     CDS             complement(6264..6950)
                     /locus_tag="U2I75_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN1695831.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            7424..7744
                     /locus_tag="U2I75_000677"
     CDS             7424..7744
                     /locus_tag="U2I75_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN1695832.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            7898..8803
                     /locus_tag="U2I75_000678"
     CDS             7898..8803
                     /locus_tag="U2I75_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1695833.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            8800..9573
                     /locus_tag="U2I75_000679"
     CDS             8800..9573
                     /locus_tag="U2I75_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1695834.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(9754..11178)
                     /locus_tag="U2I75_000680"
     CDS             complement(9754..11178)
                     /locus_tag="U2I75_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1695835.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            11389..11835
                     /gene="nrdI"
                     /locus_tag="U2I75_000681"
     CDS             11389..11835
                     /gene="nrdI"
                     /locus_tag="U2I75_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN1695836.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(11900..13882)
                     /locus_tag="U2I75_000682"
     CDS             complement(11900..13882)
                     /locus_tag="U2I75_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1695837.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(13896..14792)
                     /gene="murQ"
                     /locus_tag="U2I75_000683"
     CDS             complement(13896..14792)
                     /gene="murQ"
                     /locus_tag="U2I75_000683"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN1695838.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(14795..15856)
                     /locus_tag="U2I75_000684"
     CDS             complement(14795..15856)
                     /locus_tag="U2I75_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1695839.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(16067..16633)
                     /locus_tag="U2I75_000685"
     CDS             complement(16067..16633)
                     /locus_tag="U2I75_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1695840.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(16661..18163)
                     /gene="eat(A)"
                     /locus_tag="U2I75_000686"
     CDS             complement(16661..18163)
                     /gene="eat(A)"
                     /locus_tag="U2I75_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1695841.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(18421..19542)
                     /locus_tag="U2I75_000687"
     CDS             complement(18421..19542)
                     /locus_tag="U2I75_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1695842.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            19668..20609
                     /locus_tag="U2I75_000688"
     CDS             19668..20609
                     /locus_tag="U2I75_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1695843.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(20759..21655)
                     /locus_tag="U2I75_000689"
     CDS             complement(20759..21655)
                     /locus_tag="U2I75_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN1695844.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTSFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            21924..23264
                     /locus_tag="U2I75_000690"
     CDS             21924..23264
                     /locus_tag="U2I75_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1695845.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            23482..23925
                     /locus_tag="U2I75_000691"
     CDS             23482..23925
                     /locus_tag="U2I75_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN1695846.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(24190..25608)
                     /gene="nhaC"
                     /locus_tag="U2I75_000692"
     CDS             complement(24190..25608)
                     /gene="nhaC"
                     /locus_tag="U2I75_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1695847.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(26011..27234)
                     /locus_tag="U2I75_000693"
     CDS             complement(26011..27234)
                     /locus_tag="U2I75_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN1695848.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(27265..28104)
                     /locus_tag="U2I75_000694"
     CDS             complement(27265..28104)
                     /locus_tag="U2I75_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1695849.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(28121..28801)
                     /locus_tag="U2I75_000695"
     CDS             complement(28121..28801)
                     /locus_tag="U2I75_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695850.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(28803..29876)
                     /locus_tag="U2I75_000696"
     CDS             complement(28803..29876)
                     /locus_tag="U2I75_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1695851.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     misc_binding    complement(29932..30170)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(30212..30445)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(30522..31193)
                     /locus_tag="U2I75_000697"
     CDS             complement(30522..31193)
                     /locus_tag="U2I75_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1695852.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(31280..33958)
                     /gene="mgtA"
                     /locus_tag="U2I75_000698"
     CDS             complement(31280..33958)
                     /gene="mgtA"
                     /locus_tag="U2I75_000698"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN1695853.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            34617..35054
                     /gene="ndk"
                     /locus_tag="U2I75_000699"
     CDS             34617..35054
                     /gene="ndk"
                     /locus_tag="U2I75_000699"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN1695854.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(35076..35942)
                     /locus_tag="U2I75_000700"
     CDS             complement(35076..35942)
                     /locus_tag="U2I75_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1695855.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(36102..38195)
                     /locus_tag="U2I75_000701"
     CDS             complement(36102..38195)
                     /locus_tag="U2I75_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1695856.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
     gene            complement(38671..39294)
                     /locus_tag="U2I75_000702"
     CDS             complement(38671..39294)
                     /locus_tag="U2I75_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695857.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(39453..40850)
                     /locus_tag="U2I75_000703"
     CDS             complement(39453..40850)
                     /locus_tag="U2I75_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1695858.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(40882..41853)
                     /locus_tag="U2I75_000704"
     CDS             complement(40882..41853)
                     /locus_tag="U2I75_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1695859.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            42227..42652
                     /locus_tag="U2I75_000705"
     CDS             42227..42652
                     /locus_tag="U2I75_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695860.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            complement(43140..43841)
                     /locus_tag="U2I75_000706"
     CDS             complement(43140..43841)
                     /locus_tag="U2I75_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1695861.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(43857..44297)
                     /locus_tag="U2I75_000707"
     CDS             complement(43857..44297)
                     /locus_tag="U2I75_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1695862.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(44297..44872)
                     /locus_tag="U2I75_000708"
     CDS             complement(44297..44872)
                     /locus_tag="U2I75_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN1695863.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(45719..46198)
                     /locus_tag="U2I75_000709"
                     /pseudo
     CDS             complement(45719..46198)
                     /locus_tag="U2I75_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(46357..47058)
                     /locus_tag="U2I75_000710"
     CDS             complement(46357..47058)
                     /locus_tag="U2I75_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1695864.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(47166..48575)
                     /locus_tag="U2I75_000711"
     CDS             complement(47166..48575)
                     /locus_tag="U2I75_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN1695865.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(48623..50488)
                     /locus_tag="U2I75_000712"
     CDS             complement(48623..50488)
                     /locus_tag="U2I75_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1695866.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(50609..51460)
                     /locus_tag="U2I75_000713"
     CDS             complement(50609..51460)
                     /locus_tag="U2I75_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1695867.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(51770..52483)
                     /locus_tag="U2I75_000714"
     CDS             complement(51770..52483)
                     /locus_tag="U2I75_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN1695868.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(52551..52877)
                     /locus_tag="U2I75_000715"
     CDS             complement(52551..52877)
                     /locus_tag="U2I75_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1695869.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(53000..53227)
                     /locus_tag="U2I75_000716"
     CDS             complement(53000..53227)
                     /locus_tag="U2I75_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN1695870.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(53549..54802)
                     /locus_tag="U2I75_000717"
     CDS             complement(53549..54802)
                     /locus_tag="U2I75_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1695871.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            54863..55603
                     /locus_tag="U2I75_000718"
     CDS             54863..55603
                     /locus_tag="U2I75_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1695872.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(55689..56447)
                     /locus_tag="U2I75_000719"
     CDS             complement(55689..56447)
                     /locus_tag="U2I75_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1695873.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(56444..57472)
                     /locus_tag="U2I75_000720"
                     /pseudo
     CDS             complement(56444..57472)
                     /locus_tag="U2I75_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(57492..58679)
                     /locus_tag="U2I75_000721"
     CDS             complement(57492..58679)
                     /locus_tag="U2I75_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695874.1"
                     /translation="MKNYNRISANNQLPMIESLRVPTNADKKIYGHVVVPEGVEAKPR
                     DAWTGEVEVTLRITYADTTKSSVEITALTQGQTDENEGAGYNPWGEGEQGGLFEVQVP
                     NDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVDVIPPEPANLSTTLINAASRTIT
                     GTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPENQLFAGDVLEIVLNDQAGEATAK
                     GVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKIVVEGGLSFLAPSKLDFGQIKAT
                     GLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQPFTNDAGFSMQDVLYFKKGEEY
                     QLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLVLTKENQIAGNYSAEITWTLADA
                     PTE"
BASE COUNT        17250 a        12649 c        10341 g        19905 t
ORIGIN      
        1 gatattcttt tgcatatgat cataaagatg accactgatc atccgctgac cgacaacttt
       61 gaagtcatgg cgttcgtata attttttagc ctttggattt ttttcatcca cacttagacc
      121 gatgacggag cgattagctt tcttcgccaa tttaggtaat gcttctaata atcgcgaacc
      181 gatgccttgt cctctaaaat cttctgatac actgatagag tccaaatacc attcgttggg
      241 aaacgtttct ttatcaatga acaaacgtgt agacgcatct agctgatatt tctctaagat
      301 aggtaccagt ggctgatcga tgattgattc ttcttcatct gtataaccgt aagcaactcc
      361 tgcaacttta ccgtctattt cttctacgat acctcgtgaa taactgtaac gataatttgg
      421 atcagtgatt gcttcttcta aaatctctaa agtcttttgt tcccctgtct gcttcaaaaa
      481 tggcagttcc atatctttta gaatcactaa gatcaatggg gcgatttctg gagcatcttc
      541 ttttctcgca aaacgaatca tagtttcctc tccttttctc cattctaaca tgcagaaaaa
      601 gaaatcgcaa aatcgttatt ttaaaaaatc ttcaatcttt tcagtaatga aataaccatg
      661 agtaacgatt cttgccgctt cagttcggca ataagggcag acgactggca tttctttatc
      721 attatgttcg actacattat tgacttcttc gtatgcaaat ttttttccgc aattagggca
      781 tttcattttt tccatgttat ctcaacttcc tttcatcttt actctcttag cttactctta
      841 tattgtaaaa aagagaagca atatccttct tctatgcaga tatccatacc tgacggtcct
      901 gttctccatc tattaatcgg actttataat aaggacgttc atctttgact agaagtgtcc
      961 actctttcac ttttgctttt ttcccgtagt agctgactgc ttccttcatc gctgcttgcg
     1021 gttgtttgaa ctcttccgtt ttttctatat caaaaagatg ttctgcggcc ttttccgtgt
     1081 catttcgtac ggttgttttc ccagtggatg gattgactgc tatgacttgt ccttcacaaa
     1141 aaaactggta ttcatatgtt gcattcgtag gattctccaa atctttttca actgcaaaat
     1201 gaatagcaga tatgttatgt tcttttgttt cgttttttac gattgctgct gcttttttca
     1261 atgagatccg atattcctgg tcactaatat catccatgtg aaaagcggca ttgagcagtc
     1321 ccgtcatacc ggtaatccct attacgatga gggcagcaac accaaacatc atttttttca
     1381 ttcttcattc tcctgtttct tttttttaaa gaatatcaag aaatgaacga atgaaaaatc
     1441 gaatgaaacg aaaaaaaagt gacaattttg ttatctttat tcgtgcagtt ctaatggcaa
     1501 tccgtctgga tcaaagaaaa acgtcatttt tttcctgtaa aggtatcttc tctaatcggt
     1561 tctgattcga tccctaagga ctgcaattct gcaattgttt cttcaatatc gtctactttg
     1621 aaagcaagat gacgcaagcc acaagcttct ggatagtttg gtcgttgtgg cgcattagca
     1681 attccaaaaa tctccaattc agcacttcct agtttcagat ctaatttata gtcatctcgt
     1741 tctttgcggt aattttctct gattacttca aatcctaatt tattaacata aaaatcacgt
     1801 gatttctgat aatctgaaac aatgatggct atatgatgga tcgttgtaag gttcatatga
     1861 gcactcctat tcttcgttta agctcggatc tatattaatc tccagttcca ttgtaaatgt
     1921 gtcagattga aaaaaatagc tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga
     1981 aagtaggtcc tccaagacac tcttcctgtt cccaatataa taaatgaaag taagaaatta
     2041 tattttcaag aaaacaaaca gaaaattatc ataacacaaa ttatttataa ttgaaagact
     2101 agtttttttt caagaaaagc atacaataga ttgtgttagt aaatacagaa cacactacat
     2161 ttagtgtctt aaggagtcct catacatgaa gtcatatttt cagtttttag ctcatcagct
     2221 aaaaggctgg ccgcaacaaa attattatct tttctttttc agtcttggct gccaggtcat
     2281 gactcttgtc aatagtccca tcacttggct gtccgttatt acatttatcg gtaccacgct
     2341 tggtgtattg tgtattttat ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat
     2401 tttgtctgcg atttgtttta ttatcgttgg gttttcagca aagaactatt taagtatcgg
     2461 tgaacagatt gcttatatga tcacattgga tatccccgta ttgttaagta aagaatggaa
     2521 tcataatatg gcttcaaaaa tccgaaaatt cactggaaaa agctggttta tcgcaatcat
     2581 cagtactttc cttgtctatc ttgtttcagg attcctgatt ggtcgttata cagatgatcc
     2641 tcggccgtgg gtagatgcca ttgctttttc tattagcttg actgctggta tcatcagttt
     2701 cttgcgttac aacaaccaat attactggtg gttagcttca gggcttgctc aaatggtcct
     2761 ttggtatatt tcatttacgc atggatctgc aagtttggcg atgttcgtga acagctctgt
     2821 ttacttgctg aatgatgtcc ttgcatttac cgtttcacct tggtataacc aaaaagaacg
     2881 tgaacgtatg caaaaaatgg aagaagaata ctacaacgca caacaacacg cataaaaaaa
     2941 ctgagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
     3001 cggaatcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat
     3061 tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tctatttttt
     3121 atagtaattc cttcagatac tttcttgcct catcattgaa acgcagtgat tcttctaact
     3181 tctgataata ttccaacgct tgtttctgag cttcagtaaa acgataatgt tttcctttcc
     3241 atttgtcgat tgtctcttgc acatttccag attggatatc tttcactact agttgccatt
     3301 tttcttttga gactgtacgg tctttcttat cagcagattg tatagcttct cgttctttac
     3361 gcaatacttt taaaaaacca tttctgacta tccgaggaat gctgtcgaag aacccttctg
     3421 taaaaccata gcgacaatat tgcttccaaa taaatagata gatttttggt tggatgaatc
     3481 gttggttttt cgcaaaatat tccatctgag cagtgacttc tggtgttcgt tgacgttttg
     3541 tctttctcaa cacggaaata aatgtttcaa aataagcatt aggatgtact ggcacttctt
     3601 catatacgat ggattgccaa tctttacgta aaaatgcgcg atagttgatc aagaattctt
     3661 ctacatcctt tactaattct tctgcaaagt cgtcaaatag ttcttttttt atctgtcgct
     3721 tttttgactg cccattgatg tgttcactta ttttcttgat ttcattttcc aatgcttgat
     3781 aatccgctat gtaaatcggc atttctactt ggcgaaattc tgcgagccgc tgagctaatt
     3841 ttatatccgg taaatatggc tgtttcatcc gacgattttg gtacaagaca aaacagtatt
     3901 tttgccatag tccttcagga aaatcgatat tcttctgctt aagccataat aattcgtcta
     3961 aggccagatc aacttttgtc attccctgat ggatcgaagc tgccactgtc ggagagacat
     4021 gcaggtgatc ggcaaagtgg ttgatgccta gttttaggat ctttccggtt tttggcgatt
     4081 taacgatgta ttgcactttt aattctctac cacactcaca atacaatttt tgctggcttc
     4141 gttttgttcg aaaaaacgga ttgatcttga agtcagaaaa cacccaatct gttgcagcta
     4201 aataatgttc ttccataaaa cgagagctga ttagatatcg gcggtgctta tcgactagtt
     4261 cccgctgttt tttcgttaat tttgcataga tctcccaacg ttgactgctt gtgtattgtt
     4321 tagaaatttt gatgggatag cttttgctga tttcttttgg aaaccattct tcaaagacgg
     4381 attgccattc ttccacggct gtttctttcc tttctaatta caaaaagctg gctactaaca
     4441 taacgttagc aaccagccag tgtttcttgc taaattagtg agttgttccg gcatttccgg
     4501 aaatatcttc tttattttct actgctgctt caactgcata agtcaaagaa gaagcaatgt
     4561 cttctaaaga catagaaggc gtacccactg gacgatcgat cacttgatcg ggtgagaatg
     4621 gcacgtggat gaacccacct cgaattgccg ggaattgtgt ttcgatcaaa tgaagcaaac
     4681 gatacatgat atcattgcag acaaatgttc ctgctgtata agagatgtac gctggtagac
     4741 catgatcttg tacatttttt gtcattgctt tgattggcaa tgaggtaaag taagcggctg
     4801 gaccgttttc ttctaaagcc gtatcgaatg gctgatttcc ttcattatca gggattcttg
     4861 cttctgctag attgattgcg acacgttcga acgagactgc tgaccgccca cctgcttgtc
     4921 cgacacagat cacgacatct ggttggtgct gttggatggc ttctttgact acttgactgc
     4981 ttttttcgaa tacggtcggt acttccactt tgatgatttc agcaccgctg atttcatctg
     5041 gcattttttt tactgcttca taggctggat tcactttgtc cccaccaaat ggatcaaaac
     5101 ctgttactag tactttcatc tgttgttcct ccgtccgatt gatttaacat atggttatct
     5161 tttatcatac cattttcctt gaacagcgta aataaaggaa acaatagttt aaataacata
     5221 acagatcaaa cttaatatat tcttataaaa aaatagtgtt ccactacaat gttagaagag
     5281 gttgcgacaa ttattctgtc acaacccctc tcttctatta tttaaataga atcatataag
     5341 caatttggaa aactagcata aataacgcaa taaacaactg gtttttgatc actccgtatt
     5401 tgtctttcat ctccagcatc gctacaggca cgatattgaa gttagcagcc atcggtgtca
     5461 gcagggtccc acaaaaaccg caagtcaatg caaccattcc tattaacgct ggattcgctc
     5521 catgactaaa tacgaatggt gcaccgatcc caaccgtcat cacggtgatg gccgcaaatg
     5581 cattccccat gattaccgta aatatcacca tgccaagagc gtaaatgatg atgccgatgt
     5641 tcacatttcc ttctggaacg atcgtaccta ctgcattgga aatcactgtc ccaactccag
     5701 cgcttgtaaa taccgcacct agagaagcta aaagcattgg caacatgctt aacggcccta
     5761 ctgtgcccag catatctgct gcatcatcta aaaatgtact tggtcgatta tctcgagaaa
     5821 agaacatcaa gattagcatg gctgcaaaaa caccgacgcc cacgccgact aaagcaccta
     5881 aattggtaaa tagagcaaac aagatcgcaa agacaccgat actcaaagct ggaataaaaa
     5941 ttttcattcc tagtttgtct gacatttttt ccatatattc ttttgatgga agccggcttt
     6001 ctcctttgct gactttctta gcaattgctg gtatggtcat cgcaaagatc aagccaccgt
     6061 tcacgattgc cggaagccat cttcctcctg caataactac ccctaagacc gtccagaaaa
     6121 aggctgaccc ataacgatga ggattggttt tatctgttaa atttcttact ccagtgtaaa
     6181 tcaatacgag tcccatcacg atataaagga tctccagtaa tttatcgcct aataaaatat
     6241 caggatttgt aaaaaagtcc atcttattgc ccctttcctg gataatattt cttcgctaat
     6301 ttcttttctt taagatagaa gtagacactt gccacaacta aagccgtgat agccactgga
     6361 acttccacta aagcaagctg tcccaatgtt acgtcatatc ctagatcttt cagtgttgat
     6421 tggactagca acgcccctgc tcctccgatg aatagtactt ggccaaagaa ccagcagaca
     6481 ttttccatag ctgaagccat accttttagt tcttcttcat gcttaggatg gacttctaac
     6541 ccttttgatt cgatggtccc caatgccatc ggtaaaatga ttggacgaac aaatccagca
     6601 actccaccaa aactcacgtt aaaagccgca aagatcattc gaaagatccc ataaatatct
     6661 acaatcatac cagcagatac tttcttgaat cgcttgatca aggaagccgc tgattctttc
     6721 agaccatttc tttcaagcgt tcctgttgcc aacataatga tgataaaaat cgccatcccg
     6781 cgattgttta caaaacttgt acctaaaagt tctaacattc cttgaatgcc aagaccgcca
     6841 gtcaaagctg taacaactaa agcaaccatt acgatcaata tcgagtctaa tttaaatgca
     6901 aacccgagaa taacaatcaa gacgcctaaa aggctaagat aattttccat atattttcct
     6961 ccataaaatg acaatatttt tttaattata aagcaaaaca cataaaaaat atagttgttt
     7021 tctcacgaaa tagacactat ttttaaatat ttttcgttat ttttttatca caaaaattat
     7081 cttacttatt gtaaggtaaa gcttttttta atttattgcc cgataatata tagaaataag
     7141 gtctagttac tgtttttttt aaatgattaa taaataacaa cttctttcta tgaaattgca
     7201 aagcattcat cttcttttcc gctaacgata tatccgtcag tgcttggtac actttactaa
     7261 aaagtatgag attgccgata tctggatagg tgtgctatac tacagaaaaa gatatacaat
     7321 ctgccaccgg gtaggataaa gaggatgatc attccattag gtctttgaag ggatgatttt
     7381 cctctttttg tgtatttttt tatagttaag gaggaatgta agtatggcat ggatggaatt
     7441 aatcatagca ggaattttgg aagtattctg gtcaacgatg atgaaatgga gtgatggttt
     7501 ttctaaaatc aattattctt tttacacagt gattggcatg attgctagtt tttacttttt
     7561 gtcaaaggca atcaaaagtt tgccgatgag tctctcttat cctatctgga cagggattgg
     7621 cgcagttggt tcggtcataa ttggtgtggt cttgtttcat gataagctga atctttccac
     7681 ttggttcttt gtggggatgc tactcatcag tatcattggc atcaagatta ctagcggcca
     7741 ctagacactt ggaataagta ctgatcagaa aaaataagcc tttttattta atattcaatt
     7801 tttttatgaa aaaataagaa ataagtccaa tgacctagcg acttctccac tcatcaggtg
     7861 tatgcttgta ctatctatag aaaaggagtg aatatttttg aaaagtcttt cggtccaaga
     7921 attatccaag aactttggtc acaaacaagc cctcagcgat gtcagtttta gcttatcccc
     7981 tggcaaaatc gttggattag taggtcctaa tggcgctgga aaaacaacga tcatgaaagc
     8041 tattttagga ctgatccatt actcagatgg aacaatctgt attggtgaaa atgagatttc
     8101 tccctctagt cataaagggt tggaacaagt aggagcattg atcgaatacc cgggtatcta
     8161 tccattctta acaggctatg atcatctgaa gttattctct gaaaccaacg atgtatctgc
     8221 tatcgacact atcgtgaatc agctcaagat ggaaaagtac attcacaaaa aagcgaaatc
     8281 ctactcgttg ggaatgaaac aaaaattggg aattgctctt gccttactga accatccgtc
     8341 tatcgttatt ttagatgagc cgatgaacgg tttggatcca caagcaacaa gagatgtgcg
     8401 aaaaatcatt accgacttgg ctactcaggg cacctctttc ttgatctcca gtcacttact
     8461 cagcgagctt gaaaaaatgg tcgatgagct gatcctgatc aatcaaggaa agatcatcaa
     8521 acaatgtaca atggctgaat tgaaccaaac agcgcaagat ttcatcgtta tctctacaac
     8581 aaatgatcta gctgctcgaa cgatcttgac agaagctgga tatactttgg aagatcctga
     8641 taaaatcaag cttttaaaag aaaacgaaga attattagga accgtgatcc agctattgtc
     8701 tgcacaaaat atccatgtga cagacgtcca gcatctgacg aatgatttgg aatcttctct
     8761 attggaaatt ctggattcag taaaggggga agaagaaaaa tgacattgct tgtaaaacaa
     8821 gaattattta aattgatcaa gaaaaagtca actgctgttt tatctgttct attagttgtt
     8881 ttgctcatag gaacagcact tctagccaaa aaatacacga cgatcattga tccagttgaa
     8941 atgactgctc agctttttag tgccacatct tggattgtct ttatcatgat tgcagctgca
     9001 agcacgatca tttcgatgga agcacaatat ggtacattga aaaatctcct ttatcgaaag
     9061 tattctcgag gagaaatatt agtaagcaaa tggatcacat tagttattta ttctgtttat
     9121 ttataccttt tagctattat tgtcactgtg ttgatgaaac tgattctttt cccttcgatt
     9181 tccttcacgg agaaggtttc tactggtcaa acactgatcc aaagcttgtt cacttatact
     9241 ttgggtagtt atatcggttt atggctgatt ttaagtttag tgttgatgat tgcttgcttt
     9301 atcaatagtt ccggtgcatc catctcagca ggaatcgtct tttattttgc ttcgtcaatc
     9361 atatctggta tccttattgc actcatccaa aaatgggagt ggatcaaatg gaatccgatc
     9421 agtatgttga atctgcaaaa ccaaattgga aacgaagaga tcatgaagca attgactcat
     9481 ctttctacta atcaaatgct attcggtaat ttagcttaca tcgttttgtt cttagcatta
     9541 ggatatctcg tatttaagcg aaaaaatgta tgatgactca tcaaaaaaga ggctgtgaaa
     9601 gagacgttca gccttattgc cgattggctg tctcttaaac accgtttatt ctgaaaaagg
     9661 agctgtgaca acatttgtgt cacagctcct ttcctagtat gtttttgaat aaacgacagg
     9721 ctcacaacct ctctttatac ttcttttata cttctatacg tgaagcatcc cagtggcaaa
     9781 taaaaccatt gcaatcactc cgaccatcgt cacacctata agtaaaatct tatctaagtg
     9841 gatatgttgt acaggctcgt tttgctcctt acgtgctttg aggtaaaaat aaatgcctgg
     9901 caagaaaaga acaagaacaa gcattacttc attaaaggct acgactagca tagcccatac
     9961 ttgaaaggca cttgctaaaa gccctactaa aaattggacc cattctttcc gctgataaga
    10021 aagtttcatt tggtacaatc ctaagaaaag ccacgtgaag aaaatagctg ctgtaccaag
    10081 tgtgtaagca aagttataag cactgtcagt gaataataat gaaagtaaaa agacattcgt
    10141 acataacgtc gtcaatacga tagcataagt tggtgcccca tttttgttga gttttcccca
    10201 tgctttaggc aaaagtccgt cttcagccat gtttcccatt gtttcaatcg gtaagatcgt
    10261 ccatgagagc caaacaccaa ctaaagaaat gatcaaaccg ccgttgatca cgactgctcc
    10321 ccatttacca accatcattt caaacacata gcccatagca ggctgcgcct tgatcgtttg
    10381 cagctgattc atcgtcattg tcccatatgg aagcatggaa accaagacgt aaataataat
    10441 caagccgaat atccctagaa tcgttgcttt cccaacatct gattgttttt ttgctcgtga
    10501 acctacgaca gaggctcctt caataccaac aaatacccaa agcatcacca tgatgcaact
    10561 caatacttga ctggaaaacg ggatcggctg tccattcgca ttcaatataa tgtcgctaga
    10621 aaagttatca atgaattgtt ggatcgaaaa cgtgctgata gctactacaa taaataaaaa
    10681 taatggcact aatttacaga atgtgacgat agcattgacg aaactagcat tttcgactcc
    10741 ccgattcact agccatgcat atccccataa aaagactgta ctcatcacga tcgatgccag
    10801 attttgccca ccttcaaata ctgggaagaa atatcctaat gcgctcatca acaatgttgc
    10861 aaaagctacg ttgcctaacc agcatgtgag ccagtaaccc caaccgctga taaaaccagc
    10921 gaaacggcca aaaccttctt ctgcgtaact aaagatcccg ccttttaaat ctggacgttt
    10981 tttattgata ttattgatcg acaaggagag catcaacatt gctgttccta cgagtaccca
    11041 actgaaaata gctgggccgc cagccgatcc ggagatgtcc gtcataatac caaatacgcc
    11101 acctccaatc gctgaaccaa ttactaaggc agcaagtgga attaaaccaa ttcctttttt
    11161 ctgttgctct gtatccatgt aagcatcctc caaaataaaa ccgcttttat caaaaagtat
    11221 aacaaacctt ttttattttt acttaattaa taaaaacttt aatagaaata taattctacc
    11281 ggcttttttc tcattataca agcaaagcgc ctagtccctt tccaaattac accaccaaaa
    11341 aacttaaaaa agatgctata ctaaaaggaa agaattggag gatgatccat gaacatttta
    11401 tatatatcga tttcaggaaa tacgcgtgcc tttgccaaac atctagcaga atatgctgaa
    11461 aaaatgcatg cagaagatcc tgtcaatcct gaagttactt taaaagaaat ccatgaaaat
    11521 agtgatttta cgaaagaaac agaaccattt tttactttcg ttcctactta tttagacggc
    11581 ggaaatggtt tagataacgg tgatacagaa atcttgaccg aaacgatgcg tgaatatctg
    11641 gaacatgaag acaatcataa actttgtctt ggtgtagtcg gtagcggaaa taaaaacttc
    11701 aataatcagt attgtctaac agccaaacaa tatgcacaaa catttggctt tcctttcttg
    11761 gcagattatg aattacgggg aacaccatct gatgtgacac gaatttatca gattttagca
    11821 gaaaacaata aataataaaa aagaagaagc tgaaaaatag atttcagttt cttctttcgt
    11881 ttctattgtt tctatcatat taagctttga catccatcac aggtgctttt gcttttgctt
    11941 tacctgtttt ttccaagaca aagtttttta cacgatccat attggttacc aagacgatca
    12001 tggatgtttc tttgcctgct gctttgatcg cttctaagtc agcttctgct acaagcgtat
    12061 caggtgtcac ttgctgccct tctgttactt tcacatcaaa tggcgtaccg cctaagtcaa
    12121 ctgtgttgat tcccatatga agcaagattt ctaaaccatt tggcgttgtg atcccaatag
    12181 cgtgtttcgt tgggaagaca gagctgacta ccccagtaac tggagaatag atatgtccgt
    12241 tttctggttc tacaaagtag ccttcaccca tcatcttttg tgcaaaaact ggatcacttg
    12301 ctttatctaa agaaacaact gttccgtcag caactgatga gaggatagtc acactttcag
    12361 ctgaagtttg aggtactact ttttcaggca cttcatttac tgtggctgtt tcagtcgcta
    12421 attcttcttt ttctaaacgg tctttaggaa taccgaagaa gaatgtagca acgaagccac
    12481 cagcataagc tgccaataat ccaaggatgt agcctaacca tttaccgtca gagatcaatg
    12541 ggatcaaagc ggctcctgaa ggtccaatag cgatagcccc gacatttccg atcattccga
    12601 tcactgcccc gccgatacct ccaccgatac aagcagtaat aaatgggcgt cctagaggta
    12661 atgttactcc gtaaatcaat ggttcgccga ttcctaggat cccgactggt aaagctcctt
    12721 tgatcatttc tgttaattct ttatcttttt tgcagcgaat ccataatgcc aatgctgcac
    12781 ccacttgtcc tgcaccagcc atcgcaagaa ttggaagcaa tagcgtacga ccagtttcat
    12841 caatcatttg cacatggatt ggagtcaaaa tttgatgcag tccgaacata accatcggca
    12901 agaataatgt gcctagaacg aagccggcaa atgctccacc aacgccaagt acccagttga
    12961 tcccaccgac catgccatca gaaatgaaac ctgctaaagg catgatgaag aaaatttcaa
    13021 tcagaccgat ggcaatcaat gaaagagttg gtgtaacgat gatatctacc gcatcaggta
    13081 ctactttatg cagttttttc tcaacgattg acaatagcca aacagcgaag atgacaccta
    13141 ggatcccacc ttgtccagct gcaagagctt caccagtaaa taagtttttc agtggtgctt
    13201 ccggattcat ccctgtcagc atcacaacgg ccccgataac tccaccaaga gttggtgttg
    13261 caccaaaaac ttgtgcagca ttgatacctg tatagataac taggtagcta aacatcccgt
    13321 ttttcaagat gttcatcaca tcgatatatt gttgccatgt tgctgcatca agtgttccgg
    13381 ctgtgactaa gtttgctaaa actgcggcaa tccctgccac gatcccagta ccgacaaatg
    13441 caggaatcat tggcacaaag atattagaaa tgtcttttaa taccgatttg attttagatg
    13501 gtttttgttt cgatttttgt gccgctttca tttcttgtgc ccgttcgttt acacggtctt
    13561 ttccagatgc tgatgaattt gctgtagctg tacttacaga agaaagtttt tctcctaatt
    13621 tcacgcctgc caaatcaacc atttgctgtg ctactttgtt cactttccca ggtccgatga
    13681 tgacttgaag ctgttcatct tctactacac caagcactcc tggaatcgct tttaattcag
    13741 agacatttac aagatcatca ttgatgatct tcatacgcac acgagtcatg cagtggacaa
    13801 tgctttctac attgcccatg ccgcctgccc ctttataaat ttgttttgcc agttcttcca
    13861 aagtcagttc gttttttgcc atggtctttt ccccctcata aggtcccttt tacaaatccg
    13921 tttgcttcag ctaattttgc ttgagcttcc tctttatcac tatttgtcaa aatcatgacg
    13981 atagccagct taacattttg atctgcttct tcaaagtact tttcagctgt ttcgtaatca
    14041 gcttctgtcg cttgcatgat gattcgtttt gagcgttcga ttagtttttc gtttgttggt
    14101 ttgacatcga ccatcaagtt attgaatact ttacctatac caatcatact gatagtagaa
    14161 atcatattca agataagttt ttgtgctgtt cctgacttca atcgagtaga gcctgtcaaa
    14221 aattctggac ctgcatcgac ttcgattggg aaatccgcgt gtttactgat ttcggcttgt
    14281 ttattgcaag aaatcgttcc tgtgacagca ccgatcgatt ttgcgtaatc taatccaccg
    14341 atcacatatg gtgtacgacc gctagctgcg ataccaataa ctatatcatt ttttgtcaaa
    14401 ttcagcttga ttagatcttc ttgccctagc tcataagaat cttcggcccc ttcgacagct
    14461 acagtcattg cttcttctcc accagcaatt agacctatga ccatttccgg ttctacaccg
    14521 aatgtcggta cgcattcagc agcatctaac acacctagtc ttccacttgt tccggctccc
    14581 atataaatca gacgtccacc ttttttaaag gactcaatcg tgttagcaat cactggttcg
    14641 atacttgtca gttctttttc aaccgcagcg gccactttct gatcttcttt gttcattagt
    14701 tgaacggctt gagaaatact catctcatct aatccaaaag tttcttggtt tctgcgttcc
    14761 gttgttaggt tctctaaatt gattttgctc atttttatct tccttctttt cttataaatt
    14821 gatagttcat accagctttg atttggttga tcaatggtag ctcttctttg ataactttag
    14881 ctactacatt cactttttca tccgctggca aatcatattt ggttaattgg atctcaccca
    14941 tatagcgcaa atacttttcg ttatctaaag taatagaccc tttcatacgc gtagctgtat
    15001 ttcttgctgg gataggcgga atctctcgaa aacgtgcatc tacgcttcgt atgacatctc
    15061 ttgcatcgtc ttgccgattc gtatgcttcc ctaatatata ttcgtaaaat tcttcatcta
    15121 caggttccgt atggagcaag actgtctgtt ctttttggaa agaagcaaat tgctcctgaa
    15181 cgttttctga cagacctgca tctccgatgt aaacaatatc cgtgttactt gctaatagat
    15241 ctagagcgcc tgcaagagga tggatccctc gatgttcttc caaagttggc aatccttgat
    15301 atagtgggcc tctcaactct gtatctcctg gtacaaaacc ttggacaagg agaccttgaa
    15361 ttttgagcca ttgatttttt ttctgatacc attctttgtc cagccctgtt tcgggacgtg
    15421 gataataatt atgccaagct tcaatctgtg agaaatcagc ttctgcttct ttcaattcat
    15481 cgatatcttt aggcgtgatc gtgctagcat tcaaactgat tttcaattga tgtgaccagt
    15541 cggcaatctg ctgattggaa atatgataat ccattcttaa acctgttaca ccaattgctt
    15601 tcaaaggcgt tagatcttct aatgaaaaac ccgctcgttc caatgcctct cctgaaatat
    15661 cgaccatcaa ttctagttta ttctttttcg cttcgcttcc tagttcgatc aatcgctttt
    15721 gatatgccga aacatcatct tctggtatat gcatggaagt gaaaataccg acaaagccat
    15781 tggatgccat ttggcggata tactgtttct tctcttctga caaagaatcg ttcataaata
    15841 ttgaaaagcc aaacatatta tccctctttt tctgtatccg tcttcatttc tatgactaga
    15901 gtatatatct aaagaaacaa aatttcaata cttttattaa aacaattaaa tttcatttct
    15961 aaaggttgag tttatattat caaaagaatt gtcataacct aaaaaaggca ctgctaagca
    16021 aaagcagtgc cttgaaatat tttatttaaa taacacaaaa acataattac tcataagaaa
    16081 ccaattctgt attatctacc atgaatgtaa agaacccctc attaggcatc ggatgaaaat
    16141 aagcattgat cactcttatc aaggcattat ggcaaactaa cagatagaca ttttcttcgt
    16201 cttcgatgca ttctttcaat aaaggaacga tccgtgcata cacatctaag actgactccc
    16261 catttgggaa tcgtacagcg aacgctaaac gtgctttttg aaagttctca tctttgcttg
    16321 gcagtccatc ataatcccca aaatccattt ctaccaatcg ttcatcaacg gtcagcggca
    16381 agctgagacg atcagcaact gcttgtgctg tatctcttgc tcgttgaaga ggggaatgga
    16441 tgattttcgt gattggaaca ggcaaatcta ctaccttttc tgctagtttc tgtgcctgca
    16501 tctgtccttt ttcagtcagc ggcacatctg cccgtccgca aactaatcct tgtgcgttcc
    16561 aagttgtttc tccgtgtctc gttacataaa gcatgtgatt ctctccttcg ttggcaaagt
    16621 tccccttaac cataccttgt tgttcttgag attacaaggg ttagattctc tcaagagaaa
    16681 taatctcagt agatacttta tccaggaagg tttgatcatg ttccactagt aacatggcag
    16741 gtttcacgtt caagatcagt tgttctaatt gttcttgatt gaagacatcc aaataattca
    16801 atggttcatc ccatgtatat agttcagctg gctgtgataa agatttagcc aattccactt
    16861 ttttccgttg gcccatactc atctgctcga tcttgttatg aaaaacatct ctttccatcc
    16921 caagctttcg gaggttgttc aaaaatgctt ggtagtctac ttggtttttc tctgcaaatt
    16981 ccgccaacgt tcctcgattg tcttcataat tttggcttgc ataactaatg ctcagatgtt
    17041 ttggctggta tttttctcct ataaccttgc cgttgaatgc ccccagaaga taatggataa
    17101 tggatgactt tcctgcaccg ttaggacctg aaatcgccac ccgctgatga ggctcgattg
    17161 taaaatgaat tggctcgaat aacagatttt cataccctaa ttgaagatct tctacgctta
    17221 aaagtcgctt atggtgagac gcttggctat tcatcgtcaa agaatcgata tattcgatat
    17281 cttttaatag tttttctttc tcgctgatct gggtctccat ccgattcacg attgctttgg
    17341 atcgtttcat cgtccgtgca gcatcagcac ccactgctcc tttattcact cgtctagatt
    17401 cagtatcgat gaatccgact tgtttctttg ttttatctcc ttctcgggaa cgagaccatt
    17461 cggctttttc agctgctgtt ttctttagcc tactgacttc ttttttcaat ttttcatttt
    17521 gagccatttc aaattcatca cgaagttttt tctgttcttc atagatagag aaattccctt
    17581 gataaagttc cagttgactt ttttcgattg ctaaaacatg gtccactact tcatcgataa
    17641 atccccggtc atggctgacc acgatgaagc cttgtttttt ctttttcaaa taagccgcta
    17701 cttgttttct accagagata tccaaatgat tcgttggttc atcgattaac gggaaatgag
    17761 tgtcatccac aaataaaagt gccagtagga cttttgtctt ctctcccccc gatagtgtgc
    17821 tgaattctct ccataagatt tcaggatctg tttgcatcaa ttggagctct ctttcgattt
    17881 cccatatctc aaaatccgta atatcattta acacgtaata ggttaaacgt tctttatctt
    17941 ttgtctgttg cgggaaatag gcaaattctt gctgatggat tacttgccct tgataaggta
    18001 gtttgttttg tagaatattc agtaaagttg tctttcctcg accgtttcgt ccgataagtc
    18061 ctagtttcca ttgtgtgtca aaatttagat ttgcttgttc aaataataat gtgccttggc
    18121 tgtcgtagcc gaatgtcaga ttttttattt cgattttaga cattttccat cacctcgtaa
    18181 aaaaagacca gtcctatttt cagaactggc taaagaggat acaagggttg gatacacaca
    18241 aaaagaccca tacatcgaaa aaaccaagcc aatcccgaaa atctgcacac tgcttccttg
    18301 tctccacaag gtacagtctg aatgcagctt tcatcaatcg acttaatttt tcaatgcacg
    18361 gatccctcac tttgtcctat atttgaaatt acgatagcat aaagaaaatg attttgtcaa
    18421 ctacttcggg aggatggtga aaaatctgct aagtgtctgt cctaagattt ttgttttttc
    18481 taatgtttgc ctgtgttcca ttgcttcttc taaacctatt ggacccaaat gaatacttaa
    18541 gacaattggg agaaaatcag aaagtccttc tagctcttgg tctctacttc cgcaaatagc
    18601 gattattggt tttcgatatt tttttgcaag cactgctaca ccgtatggaa cttttccgtg
    18661 gatcgtttgc tgatcgatcc gcccttctcc tgtaatgatg acatctgctt gttcgatctt
    18721 tttttccaat ccagtgattt ggctgactaa atcgaaacca gaaaccatct tcgctcccaa
    18781 caaaaaaagg gctccaccaa taccgcctgc tgctccactt cctggaatat catctaaatc
    18841 cacctcccag aaatctttta tttgctgagc aatctttcct gcttgtttat ctaagaaagc
    18901 tagcgtcgta tctattcctc ctttttgtct tccaaattgc caagcagctc cttgttgacc
    18961 tgtatatgga ttggtaacat ctgcagcgat aatcaatttt actttagaaa caagaacgtt
    19021 tcttcgatca aaatgaagtt ttttagtaga taataaagga tttccttctg tgtacccttc
    19081 taaagaagca cccaaggatt ggagtaatcc tagaccacca tcgctacacc cgcttcctcc
    19141 taaagtgaca ataatttgtt ctacttcttg aagcagtgca tctttaataa gttctcctaa
    19201 ggcataactg gaagcaagtc ggatagtttg atccgttggt ttatcaagta gatctaaccc
    19261 caatgcttgt gcagattcga tgactgcggt gttttttcct tgccaagttg tcaataaata
    19321 gctgattttt cgctgacgga ataagagatc catcccatca atggtacgcc atgttcccct
    19381 gagcgcgtaa tagattgctt ctgcagtatt ttctccgcca tctgcaatcg gcacattgat
    19441 ttttcttcct ttacgatctt cccagccatt tagaactgcc tgatttagtt caaatgaaga
    19501 agcacttcct ttaaatgaat caattgccgc caaaatatcc atctgtatcc ctcactttct
    19561 tcttaagtaa gacacttttt ttcatttttt tcaaacaaaa caatgctatt ttcttccatt
    19621 tttctatata ctaaaaggga actcattgaa taaaggagag aaaaaacatg aaaatcgcaa
    19681 tagctggggc cggagcaatg ggcagccgaa tcggattgat gctacatcaa agcggaaatg
    19741 aggtcctctt gatcgatcgt tggcctgctc acattgaagc cattcgtaca aatgggttga
    19801 ttgctgactt taacggtaaa gaagtggtcg caaaactgcc aatctattca ccagaagaaa
    19861 tcatcgaaag caacgaacac gtggacttga tcgtagcatt aacaaaagcc aatcaattag
    19921 atgatatgtt ttgttcgatc caatccatta ttactgacaa tacttatgtt ttgtgtttac
    19981 taaatggatt aggtcatgaa gatgtactgg aaaaatatgt gccaaagaaa aatatcttat
    20041 ttgggatcac gatgtggaca gctggtctag ctggtcccgg aaaagtcaca ttgttaggtg
    20101 atggagaaat cgaactagag aacttggaac cagaaggcga agcatttaca aaaaaagttg
    20161 tagaagtctt tcaagaagca aatttaaatc cgatctatag ccataacgtt cgttattcca
    20221 tttggcgaaa agcttgtgta aatggaacat tgaatggttt atgcacgatt ttggattgta
    20281 atattgctga actaggcgcg caaaaagcgg cagagagcat ggttcgaaca atcgtatctg
    20341 aatttgcttc tatcgcagca aaagaaggaa tcatactaga tcaagaagaa gtgtaccaac
    20401 atattgcttc tacttatgat cctgacaaca tcgggttgca ttacccatct atgtaccaag
    20461 acctgatcaa aaaccatcgt ttgactgaaa tcgactacat caatggtgcc atttggcgaa
    20521 aaggacaaaa atatgatatt gctacaccat attgtgcctt tttgacccaa ttagtccatg
    20581 caaaagaagg aatccttggc gcagaataaa aggctctctg atgagaatca gctgaatcag
    20641 cagctggttc tcttttttta agcccaataa aagatcaaaa agaaaacaca actctcatga
    20701 tgccataaga attgtgcttg tttgtttcag tacataatct cttcaaaatg taggtttttt
    20761 atttgttttc aacagaggag aggtcatatt cgtattgctc ataaatagtg gaatcgttag
    20821 tgataaatcg ttttgcccaa ttttctgcat cataaaattc caatagctct gataggattg
    20881 gcttaaatga tgtatgcaga aattccggta attcatctaa atcacgttct cgaagcaaaa
    20941 aaatattttc aggacgataa aaatgataat gagcaatcgc ttgattatcc gtcactagtt
    21001 tcttttgaaa atacatggat tcaaatgtcc gcaaagtcac tccttgctga ccctcccgca
    21061 acagttccag aatcccgcgc gattcccctg tttttttcag gtactcttca taagtaagat
    21121 atccaggttg ttcattccct cgatgtggaa gaatgaaata gttggtcgta acatctcttt
    21181 ttataaattc ctgcttatag ctttctgctc gttcttttct cccattattc gttgcaccaa
    21241 aaaatagatc cgtctttggt tcgctagaag aaaaattcat atgtttcgaa taaaatgaac
    21301 tattatgttt cagcccaagt tcttttgctt ccaaaaaatc aaaatgataa atctcatcga
    21361 ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg ttctgctttg aagtgattcc
    21421 aaaaaaataa cacaattttt gcattggtaa atgggcgcac gcgtcgagcc atctccatat
    21481 taaatccata atctaaaaaa acgatcagct gataatttgc caactgttgt ctagctgatt
    21541 taatgaattc cgactgtagt aaatcttttc cggaccaaac atctaaagag cggatggaag
    21601 gcaaaaaata aagtgattta ttttttgcaa ctagcagtac gtcatttttg ttcatctttt
    21661 ccgcctcact ttctctgtca ctttttccta tgatagcctg tttaaatagt aaaaagccag
    21721 taaaacgctt cctatatttg tatgaaaatt taacatttgt atgaaaatat gatgaaataa
    21781 atcattcttc tattaaaatt cgcctaaaag ttttctgaat agttgtaatt gtctgaaaat
    21841 ttgttatcat tagtcattgt taaatattgc tatttattct taatataaat tttgattgtg
    21901 aatttaaaag aaagaggtca tttatggaag ataaaaaatt gaaaaaacaa cttagctcca
    21961 gacatatcac catgttagct ttaggtggag caatcggtgc aggtctattt aaaggaagtg
    22021 gcgaagcgat tgggatcgcc ggcccttctg tattgattgc ttttttgatt ggcgggctga
    22081 ttttatatat cgtgatgaaa ggtctgggaa aaatcgtttt aagcggtggc gacacgcatc
    22141 acggattatc tggtttgata cgcccttatc taggtgctca ttctgctgat tttactgatt
    22201 gggtctactg gtctatgtgg attatcaata tcatcgctga agctgttgca gctgcttcct
    22261 ttttgcagtt gtggttcccg cacattccag cctgggtatt tgtttttatg ttggctgttt
    22321 taactacgat catcaatctt tattcggtac ggttattcgc agaaacagaa tattggctgg
    22381 cttttgctaa aatcagtgtc attatcttac taatcatttt tgctacttat cttgtcggtc
    22441 agcaaatgtt gggcacaggc gttttcccta cccttcagca attgactgac catggtgggt
    22501 ttactcctca tggtatgaag ggaatcatca gttctttgct tgtagttatc tattcttacg
    22561 gcggttctga actgattgct atcacggtaa gcgaagcaga tgatcctaaa cgtgcgattc
    22621 ctaaagccat caaaggtgtc atgggaagaa tcatttcctt ttacatcatt cctttgtttt
    22681 tgcttttgat tctttttcct tggaatactt tagcaggcac gaatgtcagt ccttttgtga
    22741 tggtatttga aaaaatgaat atcccatttg cagcggatat cgtcaatttt gttattgtcc
    22801 ttgctttgtt ctcatctatc aattcgggtg tgtatgcatc ttcaagaatt ttgttcttcc
    22861 gtttgaagga tcgaaaaggt tcctctagaa aactagctgt tttaaacaag catcaagtac
    22921 cgcaacgtgc tgttttattt tgtaccagtg ttctttatct tggtgtagct ctttcttatt
    22981 ttgtcggtga caaattattt ggctatctgg caggttccct ttcgtataca gtattattga
    23041 tctggatcat tatcagtgcg gcatcctttg tactggcact aagaaaagga acattatggg
    23101 ataaaggaat cagctttttt gcattagctg tcttaggatt gattttcctt ggtatcctgt
    23161 tcacgaactc cattggggta actgtattga ctggcctgct ttacctgttc atttatttca
    23221 gttatcaaaa aaagaatgat gcgttcgtat tgacgaatga atgaaatcga tagaaaattt
    23281 tattttattt atgtggaata gatggaacta ttgaagaaag gagttgtgac aaaagtcttg
    23341 ttgcaactcc tttttccgaa taaacagtct catgacctct tggtaaacgg tgttcaaaaa
    23401 ccctctgtgc aaaataattg attgttctcc atgctcatga catgatagaa gaaaggaatg
    23461 aaaaaaagga gtttgtttta aatgaaaaag tattggaagt ttttgttgat gatcggtgtt
    23521 tcaacggtag ttatgtttgg tatgatgtac ttgaatgtct atgcgctcga tcatatcttc
    23581 ttcagtcgta cacgagtatt catggcgttg atgatgggtg cagtcatggc agtcatcatg
    23641 ctgttgttca tgtggaaaat gtatgaaaac aaaaagataa atgttatgat cttagccgct
    23701 agtcttcttg tttttgccgg ttccttgttt atggtacgaa gccaaactac tgtcaacgac
    23761 acagcttgga tgaaggccat gattcctcat cattctatcg ctatcctgac aagtaaacga
    23821 gctgatttaa aagatcctga agtaaaaaaa ttagcggaag aaatcatcca ggcacaagaa
    23881 gaagagatcg acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt atccgtccaa
    23941 ataaaaaaag aggatgttcc agaagtgatc agcttcaaga aataagaaga agctacttct
    24001 ggaacaccgt gaataagggg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
    24061 aaacaaaaat agcttctcct atttttcgtt tggttaggct tattaccgca gatgcaagct
    24121 tttgaacacc gtttattcgt aaaagggatt gtgacaaaaa aagtcttgtc acaatccctt
    24181 tttatgtttt tattgttcta attgcgctgt cttgcttact gcttcttgcg gtttcacaaa
    24241 tttttttgag attccccaac ctgtgaaggc actgaccatc gaaaacacag gagaaagcaa
    24301 actgaaaaat acgaatggca agtaagtgag tgtaggaata ccaaatgtac ttgctacgaa
    24361 acttccggca attccccaag gcactaagta gttgattact gttccaccat cttctagcgt
    24421 tctacctaat acagttggat caaatcctgc tttttcataa gtttctttaa atgcattacc
    24481 aggcagaata acagatagga attgttcacc aataaaaata tttactccga tacttgtcat
    24541 caatgttgta aaaatcaatt ttggtgtgtt cgtcatcttt cttgatactc gttccatcac
    24601 ggttgagatc aagccaaatt cgatcaacaa tccacctaat gacaaagtta gaataatcaa
    24661 tgacacagta ggcatcatac tttcgattcc tccgcgacta agcagtgaat cgatttgttg
    24721 gtttccagta tttgaaacaa accctgattc gatgacccca gccaacttcg ttaaggaagt
    24781 ttgtgggttt tgaataaaaa tcattcctac tgctacagcg atattcaaca aaatcgtcaa
    24841 aattgccggc acctttttcc atgcacacag taacattaaa aataaaggaa tcagtgacca
    24901 gattgaaata gtaaagtttg cttctagaac atcagtcact tcacgaatag atgaaagact
    24961 tgcggcttta cttgtgtgcc ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg
    25021 aacagttgac cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc
    25081 tgctagattt gttgattcgg ataatggaga catcttatct ccaaaaacgg cacctgaaac
    25141 aatagctcca acaaccagcg ctggatttag gcctagtgtt gtcccaatac cgaaaaatgc
    25201 aatcccaatc gttgacataa cagtaaaggc actccctaca gcgctaccta cgattgcaca
    25261 cacaacaaat acagaaggga caaaccactg gacactgatc aatttgaacc caatgaccat
    25321 tagagttgga atgatccctg cttgtatcca tacagcaatc aatgaaccta ctaaaataaa
    25381 aataaagatt gggatgattc ctggtttgat gccattgatg attccttcgt gcagccaatc
    25441 tgtcgggtat ctttttatca ctgcatacaa catgaccatt ccgatcgctg ctaagatcgt
    25501 gatattaggt gagaccttta atccgacaac gccaatcgca atgcctaaaa caataaccat
    25561 taatacaagt gttgcttcta aaaaacttgg tgttcctttt tttcccatga taatctctcc
    25621 ttttttagtt gttcttatgt tctaaaaaag ttaagatatc ggattactcc gccgcaattc
    25681 atccgctatt cctcctttat aaaaataaaa agacctgttt taaactaagg acgaatgact
    25741 ccgcggtacc accttaatta aaaacagatc tgtttttcac tttcaaactt ttattcaaat
    25801 gtatggactt tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg accaccgact
    25861 ttctgaacaa aacacctaac cgctactgcg tgaaaagttg acttgtatga agtttacctt
    25921 ccataagatc atttgtcaat gaaaaagaaa atattttttc atttttctga aaattatttt
    25981 tataactttc acacacaagt ttatttttta ctagaaagta gcaccctctt gtttttttct
    26041 tgctactatt tcttgatgtt cttgcttgat tctttctaat agctttggct ctttcaacag
    26101 atcaagagct gtattggcga gtagttctgc tgcaatcgca atagaagcaa ggcctttgtc
    26161 acttttagca gcagctttga attcttctgt gtgtccggca atatatgtct cagagattgc
    26221 taccgtaggc tggattgttg gtatgacttg gctgacattt cctacatcag aagaaccgag
    26281 acttgttctt tgttccgttt ctatttcttc atctggaaca cctgcttctt ttgcatgctg
    26341 gaagaataag tcatcaaaag aaggagtaac gatgacatca tcaactgcat tttggaacaa
    26401 tccaaactca aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat tttccacttt
    26461 ttcatatact ttatctaatg tttgccgatt tcctgcacgt aaatagaaac gtccagcagc
    26521 gtaatctgga atgacgttgg cagctactcc tccgtctgta atgatcccat ggatattgac
    26581 atcttttggc aattgcaagc gtagagcgtt gattccatta aacacttgga tcaatgcttc
    26641 caaagcattt actccttttt caggtgctgc tgctgcatgg gaagcgacac cgtaaaattt
    26701 gatgtcgact ggatcattcg ctaaagattc tgtcgttttc ccataacgaa aagctggatg
    26761 gacgcataac gctacatcga catcctcaaa aaaaccttct cgaacaaagc tgcctttagc
    26821 tgatccgttc tcgccccctt cttcccctgg ggtgccatat acacggattt ctccaccagt
    26881 ttcatcaata aattgctcca ctatgctggc ggctaatacg gagtaagtac caaacaaatt
    26941 atgcccgcat gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa aggcaattgt
    27001 gggtccgggt ttattggaaa tataacgtgc atcaaaggct gttcggtgtc cagctacttc
    27061 tttttctacc caaaaccctt ctttttctaa ttgtttaatc aacgctgctg aggaataaaa
    27121 ctcatagtta cttacttcag gatgttcgtg gatatctaga gcaagtgctt gatattctgg
    27181 taaacgatat tggattagat ccttcaattg gtttgttgag attgttgttg tcataaaaaa
    27241 ttcctcctat gctagtcatt tttcttattt tctcccgaat gtttcccatg caggcacgct
    27301 tgatcctttt gatgttttct cgattacttt gactgtgtct tctgtttgat aagcgtctac
    27361 tattttttga tacgttttat ttttttcatc ttcttttctt gcaacgatga tattcacata
    27421 aggacgtgca tggtcatcca ctggttctaa gaagatggca tcagaagcag gattaaatcc
    27481 agcatctaca gctacaccgc tattgatcac tgaaatgtct acatctgaca aagcacgtgc
    27541 tgtttgggaa gcatctaatt cagaaatgtc tagatttttt ctattctcgg taatatcaga
    27601 aacagtgggc gcttgtcctt ttgccttatc cactttgatc aatccagcag tttgcaataa
    27661 cagaagagct ctgccgccat ttgtaacatc attagggatt gctacttttg ctccgtcttt
    27721 gatatcagct acatccttca aagtgttaga ataaattccc agcggtgcat tgacagtatt
    27781 tccgatcgat actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa agaattgatg
    27841 ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc gcatttggtt gattgtaatc
    27901 tgtaaatttc acgtattcca aattaatgtt ctcctttttc aaacggtctt tcacgttgtc
    27961 ccatacatct gtatcttctc caataattcc tagttttacg gtagtttctt ttttgtcagt
    28021 tgtatctgct tttccttggc tacaccctgc caaccaaata attgctacaa atgtaatgac
    28081 tacaaaacga ataatttttt tcattttttc tcctccactt tcaatgtgta atttttttaa
    28141 tcagttgatt actgataaat tgacttaaaa aaactaagat tagaatcaat aatgtagcca
    28201 caatcgttac atctgtttgg aatcgattat accctctcga tatagcaaga ttccctaatc
    28261 cgcctgcacc tactgctcca gccatggtag tcagtccgat cacgttgatg atagtcaacg
    28321 ccgagacccg aatgatacca ggcaaacctt ctaacaaata cacacgtaaa ataataccga
    28381 ttggactggt tcccatcgct tctgccgctt cgatcacccc aggatcaact tctagcagtg
    28441 cgttctcgat ttgacgagca aaaaacggaa caactccaaa aatcaatggc actaaagctg
    28501 ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa agagaaagca
    28561 agataatgaa agggatggaa cgaatgatat tgatcagctt atcaagtatt tcaaatagca
    28621 gacggttagc tagtaatcca ttagggcgtg tcactactaa cgtcacacct aagatgattc
    28681 cgatcatccc gccaatgaat gctgtccaaa aagacatata tagtgtttgc ttcgtcgctt
    28741 cgacaaattc atccggaatc aaataaacat tgggcaaata tgttttaaag aaatcagtca
    28801 ttttatcttc ctccttcgat cactttgata cgactatcta attcgttttt ttgtaaaata
    28861 tttattttta caccagattt gatcaaataa tccaatgctt tttgccgctg tttggtttct
    28921 ccagctaaag taacgatcaa actccctaac ggcgtttctt gtaataactc tacattgcca
    28981 tataaaatat tcgctgttac ttgatatttg ctgtacagat gagcgattag aggttcgttc
    29041 gtctgatttc caatataact taattccacc agccatttat tggcgatctg atcggcaaaa
    29101 gcagaatggg caatgatcgt ttctaatgct tggtcaagat gagtagctgt tcgaataaaa
    29161 tctttggtca attcttcttc aggattgctg aagatctgga tgctactacc tttttcaatg
    29221 attcttccat cttccataac cgctaccttg ttgcagattt ctttgacaac ttgcatctca
    29281 tgagtgatca ggacgattgt taaacctaac tggcgattca atttttttaa caatgcaagg
    29341 atctgaaggg tcgttttcgg atcaagtgca ctggttgctt catcacataa taatacctta
    29401 ggttcattcg ccaaagctcg tgcgattgct actcgttgtt tttgtccccc cgacagttgt
    29461 ttaggataag cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc attcactttt
    29521 tgacgacgtt cctgtttcga ttttccagaa tactttaaag aaaaatcaac gttgtcaaaa
    29581 atagttcggc tttccattag attgaaatgc tgaaagatca taccgatggc ctttctttct
    29641 tttcgcaatt ctttgggaga gagctgactc aaattcgtct gattgacaat gacttctcct
    29701 gctgttggtt tctgcagcaa attcatcaca cgaactaacg tacttttccc tgcacctgag
    29761 taaccgacga ttccataaac atcgcctttt tcaatcgtta aatctacaga atctactgcc
    29821 tgtatctctt tttctttttg tttgaaacat acagaaacat ctcgcaattc aatcattaaa
    29881 attcctcctt atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt tcttgaggac
    29941 gcttttttgc gtgttaccac ctcatgttca agctgtgttc acacacaact cctcttcaag
    30001 tacgatcata ctctagcaag atatcggtcg cagccgtcat cgccttacat ctatgctcgg
    30061 gatgaattgc tcgatggcca ttttccaata gtcgttcctt gcttcttctc agctaccgaa
    30121 gctctctgtc aaaggttctt ctatcgtact taccatttca atgcaaataa tattcaaaaa
    30181 aaaatccttt tactaacata aactatgtca gtaaaaggac gacgtatcgc ggtaccacct
    30241 tttttcggaa gtttccttcc ctcaagccca acatctcaaa aagagagatt ggcgttcgtt
    30301 ttaacggtcg aacagttccg aatttgccta cttatcgaca aattattctc caaggccatt
    30361 ttcagctttt cttacttgac tcttctcacc agccagagcc tctctgcaaa gtttccaaac
    30421 ttactttcct tttcaacgaa atcgatattt tgttgttggg attaatctac atgaggaaaa
    30481 ctaaaaagtc aagcacgaaa ttcaaattat tttttctata tttatttttc tgttttttta
    30541 tctaacattt caataacagt tcgaagatct tttccttcag caagaagttt caatttttcc
    30601 ttttcttcga cggtataatt tcggacaaaa ctggaatcag gtatcaaaac aacgcctaat
    30661 cctgctcgtg aagcagctgt tgctcctact aaagaatctt ccactgcaaa ggctgaacta
    30721 gcttctaatc ctgttcgttc caaagctgtc agataaggaa tcggtgaagg cttgttctcc
    30781 gacacttcgt ctccgaatgt aaacgtgtca aagtaatcag ctaatttaaa gtgatccaag
    30841 atatctgttg ctcttttcgt aacagtggtc gtagcaatgc ctagtttcat atgacgttct
    30901 tttaagtctg ccaatatttc ttttgcatat ggttttaagt ggatggctcc gcttgtcagc
    30961 tgttgataaa aatattcttc acgcttcgct cggacttcct gtactttttc agggctacct
    31021 gctgctttca ctaaaatatc aaaactttgt ttccagtttt ttccgcccca agcgtcaacg
    31081 atttcttttg ggatatggat atcaaattgc tgaaatcccc aaagccagcc atcacgatac
    31141 gttttttctg tgtcaactaa caaaccatct aaatcaaata ttgcaccttt catctataca
    31201 ttaatccttt ccaaaacaat acacatagtt tagcacgact ggtccaaaaa taacagaagc
    31261 aaagaaccag ccttctcgtt tataagagtt cttggaatcg atggatatag attttcttca
    31321 cagctgtcac aagcgccaga taagcgatga ctgtagcaaa taaccataac cagtaattgc
    31381 caggaagtgg catcaagcct aattcagcac caaaacttgt aaaaggaagg acagaaccga
    31441 caataattcc taatgtagtg ataacaaaca tggcaaatgt tacgttgctt tggataaatg
    31501 gtactttcgg tgtacgcagt gcatggagca ctaaggtttg tgtccatagt gattcgacaa
    31561 accatcctgc atggaataat gcgatgaaaa tcacttgctg tgaactatcc aatgtatgga
    31621 aacttccgcc aactacagca ggacaaataa caaaatacat caacaagtaa gtagtaatat
    31681 caaatatcga acttgttggg ccaaagtaaa ccatgaattt cccaatacta gatgcattcc
    31741 atttttttgg ctcgtccaaa tattctttat ccatccgatc ccatggcaag gaaatgcagg
    31801 aaacatcata gattagattc aaaaacagaa gttgtaaagg cagcattggt aaaaatggca
    31861 ggaacatact tgcaatcagc acggaaaaca tattgccaaa attagaactt gctgtagcct
    31921 tgatatattt catgatattc ccaaaagttt ctcgtccaga aagaaggcct ctttccaaaa
    31981 tcgtcaaatc tttttctaaa aggatcacgt ctgcagattc tttcgcaata tccacagctg
    32041 tatctacaga aataccgata tcagccactt tcattgcagg agcgtcattg ataccatctc
    32101 ctaaaaatcc gacagtatga ccatttttac gtaaagctgt cgtcaaacgt gctttttgct
    32161 gaggattcaa ttttacgaat acttcatggt cttcagctac ttgtgctaat tcatgatcac
    32221 tcatttccga gattttctca ccggtaacca attcattgat ctctagacct acttgacggc
    32281 acactgaacg tgtaactaat tcgttgtccc cagtcagaac ttttacagct acaccatgtt
    32341 ctttcagagc tttcaacgct tgtttcgttg tttcttttgg tggatcaagg aaagtaagat
    32401 aaccaatcaa aaccatgtct gactcatctt tgactgaaaa ttctccaacg acgcttggat
    32461 tggtcttttg tgcgacacca atcactcgta agccatcttc attaagtgac tgtacttttg
    32521 caagtacggc ttcttttcgt tcttttgtta atggtgtaac agtaccgcca agatcaataa
    32581 atttagaaat gttcagcatt tcttcaatcg cgccttttgt gatcatttgt gttttaccag
    32641 ctggatcagc aaccacaaca ctcatacgac gacgttcaaa atcaaaagga atttcatcca
    32701 ctttttgata attgatctta tctgtatcta aagtttctgt tgcttcatca atgatagcca
    32761 catctaacaa atttttcagc cctgtttgat agtagctatt tagatacgca tggcgcaaaa
    32821 cacgatcatc ttcatttcct tcacaatcca tatgatattc aagaatgatt ttatcttgtg
    32881 tcaatgtccc cgttttatcc gtgcataaca catcgattgc accaaagttt tggatggcat
    32941 tcaaattttt gatcactgtt ccttttttgg ccatcacact tgctcctttg accaaattcg
    33001 tcgtaacgat cattggaagc atttcaggtg ttagaccaac tgctacagat agtccaaaaa
    33061 gaaaagcttc tagccaatcc ccttttgtaa gcccattgat gacgataact gctggagcca
    33121 tcaatgacat aaatttgatc agtagcatgg atgttttatg gatgcctaat tcaaagcttg
    33181 attgaattgg tttttcagat agcgtttctg ctacatgacc aaatagtgtt tcatttccaa
    33241 cagaaacaat gattccttcc gcacttccac ttatgacatt acttcccata aagacaagat
    33301 tttcgtaact tgtttcgctt tgctgatcgt cttcacatac tttcgcatgc ttttctactg
    33361 gatagctttc ccctgtcaat gctgcttggg aaatgaataa atcctttgcg gacaataaac
    33421 gcatatccgc tggtatcata tcccctgctg aaagccgaac aagatctcca cagacgattt
    33481 cttccatagg aatctctgtg tactttccat tacgttttac agctgccgtt actttcacaa
    33541 gtgatttcag tttttcagcc gcttgattgg aacgtacgga ctgaatcaac gtcattgtcc
    33601 cgctgacgat gaccatagtg aagataatgg ctacaccgaa aagatcttta tctccaggag
    33661 ctgctaatac atattccgtg atgaatgaaa taattcctaa tgcgatcaac actaatgtaa
    33721 atggcgtgat gtatgctttc aatatttcta cgatgaatgg tgtttttttg ccatatgaaa
    33781 tcgcattttc accgtaattt tctcttgaac gttcgatttg ttcttctgtc aatccatttg
    33841 ctgatgcgtg gaatagttca aataattctt gtttcgtttt tccagcaaaa gctgcataga
    33901 ttttttgtgt atctttcttt ttttccatct caatctctcc tttgtgtcca aaagacagct
    33961 gttctcgctg cttttggtct ttagctattc atcggatcga tagagaaaaa actgaaaggg
    34021 gctgagaaca gatagataat agccttggcc tttcctctag atccttgttt ttcgaatgac
    34081 tgtcatccat ttgttctcat ctcctttact aagtaatttt aattcatgac ttccaatcat
    34141 ttttcgaata gttattttcc caattagacc acctcctttt tatttttcac cgatggggat
    34201 ttgcggcaac aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa aagcatatta
    34261 aaaaaagctt ctcagatctc atcgttgagt tttgacactg tacaacgtaa aaagcagata
    34321 cctgctttta gctaccttat gaaaagcctt atctcgacaa ttcctgttct acccattggc
    34381 gtctctcgac gtttttgggc agtagcctat gtttgtatag gagcctcacc taacaaatct
    34441 tattcggttt tccttcgtta atataacaac taaaaaagag gatgtcaaat caattttcca
    34501 tgtttttcat acttttttta aaatacacac gttatattcc tgtctcacgc ggttttattt
    34561 gtctcatttt ccacctcccg ctataataga aatatagtag gaaacgaggt gctgttatgg
    34621 aaagaacact tgttattatc aagccagacg gcgtgagacg ccatttagtc ggaagcatca
    34681 ttcaacgttt tgaagcaaag ggactggcca ttgctgaaat gaagtttgaa actatgactc
    34741 ctgaattagc aaaagaacat tatcagcact tgactgagcg cagttttttt gatgaactga
    34801 tcgattacat gacttccggt cctgtcgttt atcttgtgct ggtcggcgag gaagtgatcg
    34861 atatcgtccg taagatggtt ggggcaacaa aagctgtcga tgctgttcca ggtacgatcc
    34921 gaggagacta cgcgctgccg gggactgaaa acatcattca cgcttctgat tcccgtgatg
    34981 ctgcggtaaa agagatcgcc cgctttttcc cgccattcga tataaacaaa ccgtctgaaa
    35041 aagcggcgaa gtgatacaaa ttctaaactg ctggtctagt tgctgggttc agcaatggcc
    35101 agcagttttt tctctaagga cctccgttga ttctttccta acatatattc gagcactgtg
    35161 aagaattctt ggtagtcttt cgtctttttt tcccctaaag cagcagaaat caatctttga
    35221 taataatctg ccatcaatgt ttcatagatc actgtacttc tttctttatg tgctaagctc
    35281 cgtttcatat cttcattcac actacgtgcc tttttcaacc gaccattttc tatataaaac
    35341 gaagtcaggt tcattaaaaa agtaaagaga tactgctcat gattgttcaa ctgtgctaat
    35401 cgtcgcattc tttttttgat tttcaaataa ttgttctcaa tgaaccgatc actgaatatc
    35461 caaggaaaat cagctagaat agatagttca aattgaccat agtctggtat ctgcatcaag
    35521 tattcttcaa tgattcggca ttcataacgt aaaaattcaa attctgtctc agatacttct
    35581 gttttttcga ccatgtgaag actcaaaacc aaataataca gatcttctgt tttcttaaaa
    35641 tgcctacggt tattgctgat ctctttataa ggatcaaagc tgtcttcttg tacccgatct
    35701 agcattcgtc tttttttctt taactttatt ggttcatcca gctggtttcg catccattca
    35761 tattctgcaa gtgtcatgtt cattcgctct aaaaatgatc tcaatgttac aaatgacatt
    35821 tttttcactg aatttgtctc aatcccgact agagaagtct tagaacagat tccttccgct
    35881 aattgggact gtgtcaactt cttgttgact cgaagtttct tgatcaattc accatcatac
    35941 ataatagttc tcctttttaa tttaaatttt tccacattca ataaatagat acgttaaaaa
    36001 ggaaaaatac acgaaaaaaa taaaaaaatg tttttttcat aaaaaatacc aaccttcaag
    36061 cattagaatg ttgtctaact actgaaggtt ggtttgattg tttattgatt atctttttgc
    36121 tgttcaacgt ctgttcttgt tactggatat aagttggttg ttgttttatt gccatttttt
    36181 gaaaagacac ttgtcgaatg ttcgcctttt tgcttttcga tggctatgtt acgttctgct
    36241 tcatttttat agtcatattt tttcggatcg atcgtttcaa atccgtttgg cacataaaaa
    36301 cgcaagagat tttcttgatt cagcttatct gaaagactta aagcagattt gactttttcc
    36361 tgatctttgg aaatttcctc gtcgattcct gctgctttcg tatcgatgat ttctcctgtt
    36421 tggttattgt aagcttttcc tcctaagatg gtgtatttgg gtgtcacgaa gttcccattt
    36481 cgaaaagcca ctacttgatc atgctgagga gataataaat ccgtaccaaa atggagatag
    36541 tctttatcat cgattcccaa gagatgaagt aatgtcggca aaacatcgat ttctccaccg
    36601 tattcgtgat tgactgaccc tttagtataa ccaggtatat gaatcattaa tggcacacgc
    36661 tgcatttgtg cgttgtcaaa ttcattccat gtatctgggt cttttccaag agcacttgct
    36721 aagtctttat tatctgtatt tgagatgcca aaatgatccc cataaatcac aaagatcgaa
    36781 ttttggtaaa tagcggattt tttcaggtac gtaaaaaatt gttccaatga ctgatccaaa
    36841 taatgtgccg ttcgaacata gttatctact gtgctatttc cagtgttcaa agatggaaaa
    36901 tcgaaattct tatcatccgt ataataaggc gtgtgattgg ttacggaaag gaatttcgtg
    36961 tagaacggct gttgtagatg ttcaagatat tgggcagatt cactgaataa atctttatct
    37021 aatattccat aacctagcgt ttcatctgat tcatcaaaat aagatcgatc aaagaaattg
    37081 tcataaccta agtttttata tacatgatct ctattccaaa aactagctac attcccatgg
    37141 aaaacagcgc ttgtatagcc ttgcttttgt ccaaggatcg ctggtgcggc ttgaaagaca
    37201 ttatctgacc caagttctgt gaataatgag ccttgaggta aaccaaatgt gccagtttcc
    37261 aacatatttt cagcatcact tgtctttccc tgtcctactt catgaaaaaa attatcaaag
    37321 ctgatcgttg cttgattctg gaaaatacta tttaaaaacg gtgtgacttc ttgtccgtct
    37381 accttcatat taatcaaaaa ctgctggaaa ctttctaaat ggagaacgat cacatttttc
    37441 cctttagcga tcccaaatgt ttcgggattt ggctctgcat aatgtttctt tgtataatct
    37501 aaaacttcat caatcccatt actgcttgca tgcgcgcgaa cactgctagt cattcctgtt
    37561 ttgattccat cataaatagt atacgcatcg attcctagat atttgacaat atatgaacgg
    37621 tcaaatgttc gctgcagcaa ttgtggacgg ttggcttcgc tcagcgctag atttcctgaa
    37681 aaaataagcc ctgaaagaca ggtcacagcg atagccatcg gtttgctgac aggttgactt
    37741 ttgataggta ttttcttgat cgtcaaccaa accagtatcc caatgaacgt agcaatatcc
    37801 agccagtaga ggacatcatg gggtttcatc aaagaaaagg agcttcctga taacccttgc
    37861 gacactttcg aaaatcccaa tacagatttg actgtaatga aatctgtgaa ttcccgataa
    37921 aaaataatgt tcaagtacaa caaaagcgtg tttaaaatat caatgatcaa aagaatggct
    37981 aatgctggct ttggcttttt aatatacaaa gaaaggccaa aaaacaataa ggtagttgca
    38041 atcgggttga tccacaagat aaagtactgg atcgtacctt ccacacctaa cgaaaaatct
    38101 gcatagtaag caataatcgt cttcatccag aaaaagaaaa cagctgtcaa aacgattgct
    38161 aaacgatttt ggcacaattt tttaagttgt gtcacgtata tcacttctct ctattcacat
    38221 ttttccttat tttgctcctg aaaaatgaaa attccctaaa aaagttgtta tcttaagatt
    38281 tttttattct ttctaaagat tattccgtgc acttttcaca gcataaaacc gctgtgatat
    38341 aagcgattga aacagtaagg tgataaaagt cttgtcgcag ctcctgttta gttgatgcag
    38401 gacagctttt tcacaacttc ttctttaaac ggtgttctcc tgccaactcc tcggccacaa
    38461 gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg tggctcagag
    38521 ttaaacggca agctcaagac ctcttgatac acggtgttca atcagctgac cttcggtatt
    38581 agctcaaaaa acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg
    38641 tcaaaacgct gatttcacaa cctctgtttt tcattgtcca taggattgat attgtccttg
    38701 tgatgactca ttcgtaataa ttgaggcttg attttcttca cttcccgtta tcgtttgtcc
    38761 actgccagaa tctgaaggac ttgttcctgt cgactcgttt ttttgagaag agacagttgt
    38821 tttcttgact tgttcatttg tctttttcca ttccgctaaa cgatcggcga ccactttttt
    38881 gtctactttt tcagaataag cggtgatcac ttttccattt ttcaaacggg ctactttgtc
    38941 gccatcatga gtcaaaagca aaacggtcaa atgcttgtct ttaaattgat attgtctgag
    39001 agaaatcgca ctaagtacgt catcgatatt aataaattga ttttcttctt ttccttttgg
    39061 caaatagatg tccaattcgc ctttttcgta attggcagtt attttatact ccgcccacca
    39121 ataagttgag aattcttttt tgaaattatc tactgattct ttgcttgcta cgattttttc
    39181 tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa aatgctgcgg ataaaataag
    39241 cagtaagaaa aaaccgaata accaaaagag aagaaacggt ctttttttcc tcatgctttc
    39301 ctccactcct atttaatttt gactattcta ttatatacaa aaaatgaaat agaaagacaa
    39361 aaaagttgaa aaagatcgta aataattgaa cttctttttc acaaaatggt aagaatggag
    39421 aatttcacgc atttgctctc aactattctt tcttaagcct caaatccgtt tttatctgat
    39481 acgtctttga accagtgacc cgattttttg attgttcttt ttccatcttg tgctaagtct
    39541 actgaaataa agccatatcg attcttatat gcattggtcc atgaccagtt atccatacat
    39601 gtccacatat gatagcctaa acaattggca ccttcttgaa ttgcttgatg gacatatttc
    39661 agatgagatt ggacaaattc gatccgataa tcatcgtgga tcatgccatc ttctttgata
    39721 aatcgtgctt cgttttctac acccataccg ttttcagaaa taaagcagtc gatatttcca
    39781 taatgatctc gtacattgat caaaatatca tagattcctt gttcatagat ctcccatccg
    39841 cgataaggat tcattttacg accaggcatt tcataattgt cgaaataatc ctcaggcatt
    39901 ggaccgtttt tagtatcgat tggtgtttct tttgctttta cccggcgtgg ctggtaataa
    39961 ttgataccta aaagatctac tgtattttct ttgatgaccg tcaaatcctc tggatggatc
    40021 acaggcatga gatcaagttc ttttactatc tcaatcaatt cacttggaaa ctcgcctttt
    40081 acagctggat caaggaatga acgattgaag aacgcatctg ctatttgcgc tgctttaaca
    40141 tcttcagggt ttgattcgtc tcttggatag cttggtgtca gattcaagat gataccaata
    40201 acaccacctt gctccatttc atggtaagat ttgatggcta acgctgaggc caacgcttca
    40261 tgatagccaa cttggatcgc ttttttcata tctgactctt ctggataatg ccatcgatat
    40321 aaataacctg cttcgactgg aacgattggt tcattatgag taaaccattt ttttactttc
    40381 gaaccgaaca attcaaaaca acttttggca tatttttcat aggcttcaac cgtttctcgg
    40441 ttcaaccatc cgcctttttc ttgcagatac atcggcatat cgaaatgata cagattcaca
    40501 aagggttcga taccgttttc aatcaactca tcaaaataac gatgatagaa acgaacggct
    40561 tcttcgttga ttgttccaga ttcatcaggt atcaagcggc tccattggat agatgttcga
    40621 aatgaattat gcccagtctg tttcatcaac tgtacatcct cttgatactt cgtataaact
    40681 tgtgaagttt tttcaggtcc tacttggtca aagaactttt gcggttcttt tttgtaccaa
    40741 taatcaaaaa tgttctctcc ttttccatca cctgaaatcc ttccttctgt ttgtgggcca
    40801 cttgctgcag aaccccacca aaaatttgct ggaaattgat attttttcat caaagcacct
    40861 ctttttcttt tttagttcgt tttagttgat tccatgtatg atattttcca tcacaattac
    40921 tttttcattt tcttcctctt cttttttgat tcgtttcaac agctgttctg cggcttgttc
    40981 cccgatttta tctgttggtg gtgtaatcgt gattttcgga aggatgctca aatcgttttt
    41041 atccaaataa tcataatgac taaacgcaat atccttcccg attctgatac cttcttgatt
    41101 gaaacattcg atagcaccga tcgacatatt ataattgctg acaaaaatcg ctgttactcc
    41161 tttttctaat aattcttttg ccccataata gccacttttt ctagaatagt ctcctgtaaa
    41221 gacctctaat tggatcgatg gatcattttg taccgcttct tttgctcctg ctaaacgttc
    41281 tcttgctacg tagtctgttt gagtggcagc aataaatccg atttttttat gtccttgttc
    41341 accatgatga gcgatcactt ttgctacact ttctcgatca ttcactaata ttgtatcaat
    41401 acctgctggt ccttctgggc aattcaatgc gataactgga atactcattt ttgtgagcat
    41461 ttcaaatcca gaccactctt cttcggataa gaagacaatc agcccatcga tcacatgctt
    41521 catcaggtat tcaatttttt gttcaatttt ttctggctga tcggcaaacc cgcttaataa
    41581 taagctatac ccattttctt cagctactct ttcgatccct gagacaactt ccgacccaaa
    41641 acggctggaa atcccgttta tcaaaagtcc aatagagaag cttcgattat tcttcaaccc
    41701 tttagcaatg atgttttctt tatagtccaa cgctgaaatc gcttctgcta tttttttcat
    41761 gttcttttcc ttcagttgct gaccattcaa gtaacgggaa actgttccca ctgaaacacc
    41821 agccatctta gccacgtcac ggatcgatgc catacattct cactcctgcc agttacattt
    41881 gatttctctt tcttgttgtt cgattatgat gaccgctttc tttgcatagt gttgcttgtc
    41941 atcaatatcc atataactaa agtcttcagt agagcaactt cctctcacta ttaattatac
    42001 ttaaacaagt atttgaaccg gttcattttt aatatagcat ttttttaaaa gattgcaaca
    42061 cttttttaaa acgctttcct aaaatggcga attttttgcc tttttaaaat tttattctaa
    42121 aatttactct atttgtgggt gttttgtgcg tttttctctt ccatccgttt gaagttttac
    42181 tttccaatgg tataatttct ttatcctatc aaatggggga agtattttga agaaattaat
    42241 cgtactattt attttgtctg cgggactttt gacattgact ggctgtaatt tgtttggcgg
    42301 ctctgaccaa tcctcggatg aaacaaccga ttcttctgtc ttaaaagcgg aaaaagcaga
    42361 tatcactaac agtgtatata cgctaaaagt acgtaaagat atggatttat ccgcgggaca
    42421 gttgacattt acagataaaa aaatggaatg gacacgcacg tatcagacac aagaaactga
    42481 ttcttctgaa tcatctgaag aaaaaacagt gctgacagac atcaagatta ctaccaaaga
    42541 tgacgaatat attatttcag gaaaagaaaa taaaaaagaa gcaaccatca catttaccaa
    42601 aatcggtaat taccgtttga aggatgagga tggcaacatc tacagtctat agtgccgtct
    42661 gttggatctt cttttgtttc tccccttctt ttctgctata gtaaagaagc aagatgattg
    42721 taaaggagac gaaatggatg atcgatagtc ctgattattg tacatgtaat aacaacaaaa
    42781 atatccatac agaatttaat gaccaaggaa gcttgagcat atgcgacaac tgtcacaaac
    42841 tagtcaaaga ttcatttaaa acatttgaat cttatcctgt tcaaaattat gtaaaaagta
    42901 gaagaagata aacaaatcca agcaaaactc actatggctg atttttatga acttcaaact
    42961 cagcttcttc ctttgatcaa gcttcatcag taaaacaaac agtggatcat ttcagtttac
    43021 aaatcaaaga gtaaagattt atttatttca aatatttgaa tataaaataa ccaaacgata
    43081 gaaaggctta ttctacatca cgtagtaatt tttctttccc gtcgtttggt tttttatttt
    43141 tagcctaatt cttgaccttt tgcatgctgg ttatccgtaa aatattttcc agcaggcttt
    43201 ccttccaatt cagctagttt ttcttggatc tgatggatca ccttttttgc atcttctgga
    43261 ttttctacaa aatcgaattt atccccatcc acttgtattt taggacatac atcaaaatct
    43321 tcataccatt gattatagcg ggtgttcaac gtttcataat aggtatataa cgtctcatcg
    43381 ctttctagct gttcatagtc acgcccacgt tttttgatac gttctagcat cgtgtccaaa
    43441 gaaactttga tatggaccat caaatcaggc cgtttttttg gagcaacatc atctaactcg
    43501 tttagcatcg tatctaataa gctatcgtac tgctgtactt cgatatccgt tacccgacct
    43561 aaatcagcgt ttagatggaa caacaggcta tcttcataga tggatcggtc tagcacattg
    43621 tcatcgttca ctaaagcatt tttcattgct aaaaatcgct tgttcaaaaa gaagatttgc
    43681 aataaaaaag cgtactgctc aggatttgaa taaaagagcg gcaatacttc gttatcttcg
    43741 acattttcat aaaatggttt agaatcaatc tcttttgcaa gcatatccgt caaactgctc
    43801 ttacctgcac caatcgttcc tgaaatcaaa atcactgaca tacaaccctt cctcttctat
    43861 aagtactcgc ctacgtaagt ttcaacaggc aatgtttcaa aatcatattc acgtacagct
    43921 tggtctgatt ttaaatacgt atggagactt tgtgtaatca tcaagttcgt aggtaccgtc
    43981 tcttcttgta caatgatcat cggttttttc aacatagttg ccacacctaa ttcgtaagcc
    44041 gttcctggat cgattgtttg atgctcaaag tcaaggatag caacaacaaa ttctgcattt
    44101 gtcagttctt ccatatcttt ttcgtatact tcctgcgccc atccagcgct gaatagttca
    44161 taattgcttt cttggtgatg acgtggagag tagaaacttg tgactgtttt gttttcctcc
    44221 aacgcttttt cgatacgact tactcgatcg atttgctcct ctgaaaaaaa tggtcctgct
    44281 aaatatattt gactcattat tttgcctcct tcaataataa ctgattttta atagatactg
    44341 ctcgtaattc attgaccagt tccaatggat tgttggaaaa gattacttga tccaaccatt
    44401 ttttatccat aaaatcctct tttgcaaaag tttctaataa ttgttgcaag ccgctgtaat
    44461 aaccgccaat attcaatatt ccgattggct tgtcgggcgt cagaccgatt gctatccaag
    44521 aaagaagttg tgagaattcc tccaatgtac ctagacctcc agggaaaacg aggacagcat
    44581 ctcccttttc aaataataat acctttcttt catcgatcgt ttctgtagga ataaatgtag
    44641 taacatcttt tccaggcaat tcagtttcaa aaagcccatt cggatagata cctgtgactt
    44701 ttccgttatt ttccagtact gttttactaa tcactcccat aatcccggag tatgaaccac
    44761 cgtaaaccag ttcgatttct tcttgtgcca tgtattttcc taacgtttgc gcaataaatt
    44821 tgtaactttc tttattacca aaacgggaac cgcaaaaaac agtaagtttc ataaaaaaac
    44881 actccctaac tcaaaagtct atcttcattc tacacgtgtt tttttaaaat aaaagactag
    44941 attttgtagt tttttagtgg tatataggat agacaccact atatatagta tttagaaaaa
    45001 aacatttttc agctatttta tagataaaac aaaaataaat aacgtgtttt catcgatgtg
    45061 aaatgactta cttatagaag atactgggat ttcgtcctat atgccaataa cgtgtattat
    45121 tttaaacaac aaatacttgt ttcttttatt aacgaaaaaa tcattttgtt ctcgtgttat
    45181 gtttcactga taaatgatgt ttctctaaat aaaaaagccg acgaactaaa cgatcaaaat
    45241 ttgatcattt ttgttcatca gcttctccta aaaatctttt tgttaacgat aaatagctgg
    45301 atttgtaaaa atatcacttg aatcgtcact gctggaagaa aattcagtga taaccgcacc
    45361 tgttgcgcct gcctggaact agtgtttgat attgggctcg aacgtgtact gatctctagc
    45421 atgaagaata atttcttttt ttactgtata ataactttct ttcctttttg gaatagtaca
    45481 ggaaagtttt tttgtttcct ttccttcgac ataaggtata ccagaccctt tcgacaacga
    45541 aaggttctca tttttccttc atcgtcattt caaggagggt gccgatgctc tggacagttt
    45601 cgatcgctcc taaaccaaaa tctgtatatt cgattctttc taactctgat tctttcatac
    45661 cattcctgcc tcaaggtaca tctattgtac ttttttcttg taatccactc tgcctctcct
    45721 aactaattgt caaatcatcc caagatatgt cttcttcctc tttttcttct tggatgattt
    45781 ctgcattcgg atctactggt tttttcaata ataaaatggt tattcctgtg acaaaagaac
    45841 cagctaacaa tgcaccaatg aatgcccatg gtctggtcat cgtagcgact gcaaacattc
    45901 ccgatgccgg aatacggcta tctgcgcccc aaaacatgct taatccgcca cctactgtcc
    45961 caccgactcc cgttgctatg acattgcgtg ccaagtcgtt caatacgata gggaaacatc
    46021 cctctgtgat ctgacagatc cccataggaa aagcggtctt caatgtatcg atttcttgac
    46081 gattgagtat atttttacga atgattttcc ctaagaaata agcgatcgtc aagccgaatg
    46141 gggttaccat cgaagctaaa atcaatacag tgattggttc atagattcct tctgaaaatc
    46201 gtgccacacc cccttatcca gaataaatgg taatccagaa acagccgtgt atctgcggta
    46261 ataagcctaa ccaaacgaaa aatatgagaa gctatttttg tttggctgtt cttattactt
    46321 gatgcagggc agctttttca caacctcttt ttttatttat gtttcataat caatatggta
    46381 aggatgcagc tccgtatggt agaccgcctc cgaatattca atcatttccc caatttgcgt
    46441 agacgatttc cgtatccgtt tgatgccgat ctgtgcgtct gttttcagaa ttccttgttc
    46501 ttcttctgtt aaaaatactg ccgagaaatc atcagaaaat gagtcaatag tatatccttt
    46561 tttcgtcagc aaacgataca aagattcttt gccaaattct tctctgctga attgcatcat
    46621 ttcttgtgga agataatgaa caaaataaat ataaggctga ttgttcaaat aatataagcg
    46681 cttaaaacaa acagcttgct caccgaaaaa ggaaaacaaa ggatcttcct tctttatatg
    46741 gaccaattgc aaatccagcg tttctttccg gacttcatat cgggagttct ccaagatttg
    46801 cgtaaagctt gccgctttgg ataatcgatt gtaaggacga ttgcttagaa ccgtcgtccc
    46861 acgaccactt ttctttgcga catattcatc tgatgccagc agttcgattg cctttcggac
    46921 agtgattttg ctgacttgaa acagttcttc taattcactt tctgtcggca acatcgaacc
    46981 gactggatat tttcctgaga aaatatcttc tttaatttta tctgcgactt ccaagtatag
    47041 tacacgtgtc cttttcataa cgtcaccctt tgctttacta ttactaaaag aatagcacat
    47101 aaaaaataaa aaaagaacac aaatcacatg tgagaagtgt gttctttttc attcagctgc
    47161 tttaattaca agtttacgcc atttgaggca attacttctt tataccaatc aaaagatttt
    47221 ttctttgatc gattcagtgt cccatttcct tcatcgtcta gatccacata gataaagccg
    47281 taacgtttac tcatttctcc tgttgatgca ctgacaagat cgatacatcc ccaaggcgta
    47341 tagcccatca aatctacacc gtctgctact gcattagcca tcgcttcgat atgacgacgt
    47401 aaataatcga tccgataatc atcaataatc gtgccatcct cttccatatg atcaatggct
    47461 ccaagaccgt tttcaacaat gaataatggt ttctggtaac gattgtataa ttcattcaat
    47521 gcgattctca gcccttctgg atcgatctgc cagccccatt cacttgcctc aagaaacgga
    47581 ttttttactc ctcccagcaa gttcccttct gaagttgctg cttgtggatc agtctcattg
    47641 attgccgtag acatataata actaaatccg atgtagtcca ctggatattc ttttagcaac
    47701 tggaagtctt tttccgtttg ctctaatttt tccggatcga ctccatattt tttatgggta
    47761 cgcgctgtgt atgcagggta ttctccacgt acttggacat ccgtgcagaa aaaattgaat
    47821 tcttggtttt ggatcattgt tgctgcctga ttgactggat tggcatcgat gctgtaagtt
    47881 gttgcataga taatcatgca gccgatttgg atatccgaac gcaattcgtg gccgattttt
    47941 acggctttgc tacttgcaac gaattgatta tgccatgctt ggaaaatatt ttggtactct
    48001 cctgctccat tgctttttac taatccttga cttaaagctg gaaagtgaaa ggccgaattg
    48061 atttcattga aagtcatcca atatttgact ttactgctgt atctctctaa tactgtctga
    48121 gcaaatcgtt catagaagtc gatcagtttt cgatttttcc agccaccgta ttcttttgcc
    48181 aaatgaagag gcatttcgta atgagaaatc gtgatcacag gttcaatatc atattttaag
    48241 cactcatcaa tcagctgatc ataaaattct aatcccgctt cgttaggagt accttcgtct
    48301 ccatttggaa aaattcgtgt ccaggcaatc gaaaaacgat aacatttgaa tcccatttcc
    48361 gcaaacaagg caagatcttc tttgaaacga tcgtaatgat cgattcctcg atgattcggg
    48421 taaatatatt tttcttgatc aatcgtccag tcaaattctt cactgccgat aatcgcaaag
    48481 cgctgcttac ctccaggcat agcatctgca acggatagtc cttttccgtc tcgatcataa
    48541 gccccttcta attgatttgg cagcagttgc tccaccccat aaaaattttt ctgaaaaacg
    48601 tgattgcatc ctgatttcct ccttatttta caattgccag aacatcttcg tttgcaataa
    48661 tctctttttg gtctaattca agtatatcgg caaatgcatc agtattcgta atgacaactg
    48721 gagtgattac gctatagcca gctgctctaa ttgcttcgat gtcaaaacga atcaataaat
    48781 ctcctgcttt gacgagatcc ccttgtttga ctaagatctc aaagccattc ccatttaatt
    48841 ctactgtatc catcccgata tgaatcaaaa tctccgctcc gtcagtcgtt gtgatcccta
    48901 cagcatgtcc agttggaaaa atagtcgtga tctctccgtc tgcaggtgca actagttctc
    48961 ctacttctgg atcaatagcg ataccttttc ccatcacgcc agaagaaaaa acttggtctg
    49021 gtacttcact caatggcagt actttacccg ttaaaggact ttttaaggta tgccgtgctg
    49081 aagtaatcgg ttctcctgca ttcgcatgtt tgttttctaa ttgattgtct tgtgtttgtt
    49141 cgtcaaatcc taaaatcaat gtacctacga atgcaagaac aaaagcaatt cctgtagcta
    49201 ttactgctac tgtcacattt gactctacac catccaccgt attaatgaaa gtcggaatcg
    49261 tcaataagct gaccaaactg ctggagaaag cttttactcc ggaaaatccg ataatagctc
    49321 caccaatacc tccagaaata caagcggcaa tgaatggttt cttcaaaggt aatgtcacac
    49381 catacacagt tggttcagtg attccaaata acgaagtcac tgtagaagaa atccctaatg
    49441 ctcgaagttt cgtatctttt gttcgcaatg ctacagctaa agcagcccca ccttgtgcca
    49501 agatggctgg aagaagcatt ggcatcaata catcaaaccc atattgttcg atattcaaaa
    49561 acatgatcgg tacgaatccc caatgcatgc cgaacatcac gaatacttgc cataaacttc
    49621 ccatgattag acctgctacg attggactaa atccataaat cgagttgaag agaccgccta
    49681 aagcattacc ggcaaccgtc ccgattggtc ctaaagcaag aaatgttaat ggaaccatca
    49741 ctactaaaac gacaagcgga actaaaatca tttgcagaaa ttgcggaatc acttttttta
    49801 cgaatggctc taatttactt tgaacccata cggctagaat gattggtatg acagaagaag
    49861 tataaccaga tggtgaaaga atcactggga tcccaaagaa actgatagct tctcccgcac
    49921 cagcaactgc tgtgatagct ggatacacaa gtgccatagc aattgctact gctaaaaact
    49981 ggttggcttt gaacttttta gctgctgtaa aagcaagcat gacaggtaag aaggtaaaga
    50041 caccatctgc tgcagcaaac aatatttgat aagtaccgct atcagctgtc agccagccca
    50101 tcaccgttgc caatgataag aatcctttca ataccccggc ggctgccatc gcccctagaa
    50161 aaggtgtaaa tatactagag ataatatcga tcagacgatt caaaatattt ttggactctt
    50221 cttttccatc ctccgaaact gcaagacctt cctcatccac gatcgattga taaacatctg
    50281 ctacattact gccaatcact acttgatact gaccgccact ttgtacgact tggatcacgt
    50341 ccgggtcttc ttttaacttt tgcgtatctg ctaaagattc atcttttaat ctgaatcgca
    50401 accgcgtagc acaatgtact aaacttttga cattcttttg tcctcctacc gcttcccata
    50461 cacgacgacc aactgcttga ttatccattg ttttttcctt ctttcttttt tatttaaaaa
    50521 ataaaaaccc agattgaaaa aaacagtcac aaacattcgt gaacctattt tttcaatcca
    50581 ggttttgcct gccatacagt aacaatcctt atgattggat gctttttttc tcacttacta
    50641 ctctttgaat atgaatcgtt aaataaatct gctctgcgat ccccatttcc tgtttttgcg
    50701 tttgtcgaag atactcattg atccgttcga cacaatgaaa cgcttttgga tactttcctt
    50761 gaactaatgc gtagagaaat tcatcttgct cgtcagatcc ttcccctttg agataacgct
    50821 gggcaaaaaa ctgcagatga gtaatgatac gctgataatt caaagattct tcatcgaaca
    50881 cgatgccgaa gaatttactg atgatactca gtatatctct taccattgtg gtagcggtca
    50941 tcgtcacttg catgtcttcg tttccttgtt ggctattcac aaaatgaaag gcaataaaac
    51001 cggcttcatc ctcagaaagt tccacacccg tcttttgttt cacgaacaaa agagcctctt
    51061 tcgctgcttg atattctttc ggataaaatt tttttgtttc aaataataat ggattaggca
    51121 gttggatatt ttcttttgct cgtgtgatcg cataatgaag atgatcggtc aatgtcagat
    51181 aaatattagg cgaaaattca acttttagtt tatcttcagc cagttcgact acttgtttga
    51241 cccattcgat ctcctctgcc ggaatatcag ccatcagctg ctggaattgg tttttagcaa
    51301 aagaatctct taaaacaaat tctttttgga ccgcttctgg cgaaatctcg tccccaatct
    51361 ttttttgaaa agctagtcct tttcccatac aaacgacttc ttccccgaat ccatttttcg
    51421 aaatgacgac attgttattc aaaattttat caattttcat tttgcttcgc cctctcaaga
    51481 aaaataaaaa aacctagaat tcgcctactg tttcttagct gaattctggt tttgcctgcc
    51541 gtacagtaac aatccatttc ttattcaaaa tcatcatacc atcgaatttt tatttatgca
    51601 agccttttca tataaaaaat aaaaaaactg gaacacaagt ctatctagaa aacaaccaaa
    51661 tcagagatgc acctctttgc gtttctcttt ttttcagcaa attggttttt ccttatcgat
    51721 tcatatcctt tggctgtttt taatctgtaa cgacttatgt cccagtatcc taaaatgttt
    51781 ttgtctctag attgaaagca gatctcatat catctgtcgc atcttttatc gatgtaacac
    51841 tttccagttc tccttgtaca actagtcgcg tgacggcgtc catgtcgcca caagtaatta
    51901 gtgtgacaat ttcttttccc tcgacttcat tcagtaattg ggtatctgta ggagcaactt
    51961 tttctttaaa aaatgttttg tatgtataca cattttctaa atctgtcaaa tagatcttgg
    52021 cacccatctc taaattttct agcggtgtaa acagcaattc agggttcgtt gcacgatggc
    52081 ttgctaaggc ataatttcct tttcccattt cttgatctgg agataatgtt cctgctccgt
    52141 aaagtagtga cgtattatcc aagcctttga aaaccggtaa attgattgaa actgaaggga
    52201 ctgcgatact agcaatcact ggtagatctg ttcctctcat ttgcgacctc atgacccctt
    52261 ctgtcgtcat tggttctacc gcatcaaagt caaaactgac atctttatcg ttattctttt
    52321 tcagatcttc ttttgttaca ttcgctatag catatttatc tcctgtctct ctgacaaaat
    52381 aatccttgat ctgttcattg aaaatcaaag ctagtcctac taataacaag aggaacaaga
    52441 ggatattgac tagccatctt tttgcttttc cttttttttt cattttctct ccctccagac
    52501 tcattttact gtttttcttt tgctctttcc aacagccgaa tgacacattt ctacaactct
    52561 gtgtattttt ttcgtacacc tgcgcttttt tctacaggat atttcaaatt atttttgatc
    52621 aatttttctt caatgatctc atctagatcc aggtgaaggt tgtctgccat catgtatgaa
    52681 tagatcaata catctgccag ttcttctttg attcgttcta attgtgtttc ttttacttct
    52741 tccggcgttt tccattgaaa aagttctaac aattcagatg cttccaaaga aatagagata
    52801 gccaaatctt tttcgttatg aaattgtcgc caattacgtt catctctaaa ctggttgact
    52861 ttttccatac tattcatttg cttgccttct tccaatcact tcttacttga ttaatcatac
    52921 cattttttta cataaaaaat agatgatgct tttaaaaaaa ctcctgtttt cttttattta
    52981 aaataaatgt catagaaaat caataattta ggtagccacc atgtgtatgc agtagcgtgt
    53041 cttcttccct tgaggctttt tgtttttcga tacgttcttc ttcccaaaca gacaacgcta
    53101 ctagaatctc tcttaatgat tttccttctt cggtgagcga gtactctact ttaggcggga
    53161 cttggttata ggcttttcga tggatacttt catcttgttc taactctctt aactgctggg
    53221 tcagcatttt ttttgtgatc gtagggattt gcttgcttct ccacgcctaa aataagcgat
    53281 attgctcttc cctttatctg gtgtaagcaa agcagttagc attttgacga tcgtgggttc
    53341 cgcttttcac ttcttatcta cttagtatca tgaagtatac tttcgtacta atactaagta
    53401 gcggaagtgt caactacttt gtttaactac atagcaaaag aaacagaagg gtgacaacgg
    53461 tcatcacgct tctgtttctt ttgaatatgc tgtgttgttg tctctatctc cgtgtagact
    53521 ttcctcaaac ttatcctttg acagtaagtc agtaggctga tttgctcttt ctagatgcca
    53581 gatacatttc tacaaaaaaa tagtgattct gttttgaaaa ttcgattttt agttctgcat
    53641 tttcgtaaac atccaacatt tttttcgcac tgtatagacc taacccgcca ttttttttag
    53701 aggtttttcc tcttttgacc atctccgata gcgtcaaatt cgttggattt gccttgttcg
    53761 ctatactaaa acgtgatccc tctttttctt ccagcagatt gatcgtaatc ggactctgat
    53821 cggctacacg atgatcgaat gcgttagaaa taaaaataga taaacagcga ataaaaacaa
    53881 ccagatcaat cgggattgtt ttcacttcga caggaataac tacttggatc gggattttct
    53941 ccgattctgc tttttctaga aattttaaaa agacacccct gacagctggg gatacaatat
    54001 ttgttagctg ttgtatttgc ggtttttcaa aaatagctgc agaatgatct tctaatattt
    54061 ctaaaaattc caacgtttct tttttatttt cctgaagagt aatttttaat gaaaacagta
    54121 gacttttata atcatgtttg aattcctcta gatgatggta atattcttca tttatttgat
    54181 acagttgaag cgattcctga tgttgtcttt cttttacttg gaaactgatc aacataatgg
    54241 aaaaaaacaa agaaaaaaaa ctgatgacta tcgacaaaag agatagaagc agataattag
    54301 atgagcggaa gtgaacactt cccaatacca agtaaatcac gccccagctt aataaaatac
    54361 aaatacctcc aaaatagtgt tcagttggat aagtaaaaag caagttgaga aatctcgtct
    54421 gtttgtctat actttttact aatagataga aaataaatgc gataagtagt gcaaaaaaac
    54481 ttgctgttcc aagatgtctc tttcctaaat aatccatcag atcaaaaacc agaaaaaagc
    54541 tgagtccagt gatagcaaaa aaaagattac tgatcaaaca gctgataaac aggtttttcg
    54601 tagtaaaata aaaaagaaac atcaataata agccaactag gtaaggaaag ctcactttag
    54661 ggataggaag taaaagtgtc accaccatca aagctataca accaaagctg ttttttacta
    54721 gcagttgttt cgaaaagaat aaatagatac ctaaatctaa aatcaaagta aaggtcaaat
    54781 aaagtgtcac tgttgcgacc atcttattcc tccattgttt tgtttcaaaa tgtacacata
    54841 tttttttaat aggagcctgc atatgaaaaa gtattttatc atagaagata accagtttca
    54901 cagcgaatgg atcatcagag aattggtcaa tcatcatatc gagaatatcg tgcagctcac
    54961 ttctatctct caaatcaatc agttgatcca gttgggcact attgataatc acgatgtttt
    55021 ctttatcgat atcgacttga agcaatcact cacaggtatt gatgtcgccg aacaaatacg
    55081 taaaaaaaat gcacatagta atattgtttt tttcaccgct tatacgaatt ttgcagtaga
    55141 gatcatcaat cggaaaatcc ttccgattgg atatatcaga aaagacagga atttatctga
    55201 acaaattcgt caagtgctag aacagatcaa ttggaaagaa cagcagatcg taaatgtcga
    55261 taaaatcttt attgatcaaa aaggtggaag tctcgtcctg tatcctcggg aaattttata
    55321 tattgaaagt atcaaaggtt tgaaaaacca agttatggtc aaaacaacta cagaagataa
    55381 attagtgaat acttctttaa aacaattgaa gacagagtta gaaaagtatg cttacttttt
    55441 acttttaaaa tcttactgta tcaacttatc gcaattgcag aaaattgatt ctgctcatta
    55501 tgtgttagaa tttttcaacg gtgactcctt actcgttggt cgaaagatct ttgaaaagac
    55561 aaaggaacgt ttccatgact tccaaaaaac tgcttcgagt tgatgaataa gagctgagac
    55621 gtaagcatag cagatttcag ggagtgggcc ttgaatcggc tccactccct gaaatcgttg
    55681 atatgatttt acgcttcctc ttctgtagga gcatctgtta acgaccatgt gaatgttgaa
    55741 acgtagttgt ctacagcata attggataca ggaacaaata attccactga ttgtctccct
    55801 tgttccacat tctctcctaa tgaataaatc catgtgccaa tcccttcatt tgctcctgca
    55861 gttactagat ctactgcctg atctgctggg atttcgattc cttcagcgcc agttccagaa
    55921 acggtagatg gcattccact gctattagtt gcgctatttc gaatcacggc gtttttgaat
    55981 ttcacctttg aaccagttag tttattcgta tcagatgttg taccaataaa ttctgttcgt
    56041 ttgactgata atttccaacc ggcataattt ccgcttttat ccgttacttg tacgaaattc
    56101 tgtacttgtg tttgtgctcc agtactgtcc gttccgcttt gtaatgcagc atatactgtt
    56161 tttgctgtac ctaacttgat tgttccaaaa tcaagatctg gagcttggtc aatcgtcagt
    56221 aatccggatg ttccctgatt tggttcgtca ggtgtaaaag gagtatcagg atcatttgga
    56281 tcaacaggag gaactggaac aacgtcttca ggatttgcag catctggttt ggtcacagaa
    56341 atcgttcctt ctgtagtggc tgagctctta ttctcagcaa aaacactttg tgtacataat
    56401 aatagacttc cagctagaac ggtacttaat gcaattgttt ttttcatttc tttttccttc
    56461 tttcttttaa aataaataat ccagcaaata agccaataag aatgactcca acaagtatca
    56521 gccatcgcca gtcaaatgtt tctggctgat tttttaaatc aatatccttt tcattcaatt
    56581 ggtccgctgc atcatcagtt atttcaaaat cggtatggag attccagcgt ttttctttcc
    56641 aagcaccctt cacaaccgct tgataatgtc ccggagctaa tttttttcct tctaatggga
    56701 caagtaaatc aaaatttgta ttgggtgcta catctatttt ttcttttttt attgaaaggt
    56761 aaggctcttt tgtttctttt ttgtagattt caaccgatgt agtcaagccg aacatataag
    56821 aagcatttgg attttgaaga ttcgccttaa tcacatttct tccatcgctt tgatccgcat
    56881 aagctttttt taatttcaaa tctggggaag actctttttc gctatttcaa atcaagattg
    56941 ctttcacttg agaaaactga ttgacgacac caactgtact tttgttagaa acgttctctt
    57001 ttttttcttg aaacgtgatc cctcctgcga ccacaccagc cagctctttt tctggcgctt
    57061 tcagtgaaag tgtctcagtg accgcacttt gaggaggaac agttatttct cccactgttt
    57121 ttgctaagtt tttcatatca taacttaatg tttcatcaaa tttacttttt gattctccat
    57181 attcgatcac accgtttgaa ttcgtcgtag cggagtggat caatgtctca actgttactg
    57241 atttatctgt tcggttaaag accgtgtagg ggatttcctg tgttttccca ggctccatcc
    57301 ttacatttat atatgttttg ttcttatcaa tctgattagc cggtaattca aaatctaccg
    57361 caaaattgaa ttcggaggca tggacaggtg tctgccaaag gatgaatcca aggagaagga
    57421 tcataagtaa agagcagttg tttcgatgac ttttattcca tattcgattc atcaatcatc
    57481 ctccttgatc tttattctgt cggcgcatct gctaatgtcc atgttatctc tgctgagtaa
    57541 ttaccagcta tctgattttc tttggttaag acaagtttaa acgcccgacc gttatttaaa
    57601 tattctgaat aatctgcttg cacattcgtt gaattttgtc ctctgacaat ttccagttct
    57661 tcttcattca ctaattgata ctcctctcct ttcttgaaat ataggacatc ctgcatagaa
    57721 aatcctgcat catttgtgaa aggctgagat aattttatcg ttaagatcca cggatttttc
    57781 gtttctcgct gatcataaat ccataatttt tcctgtatat ccatttggcc aaatgcttct
    57841 tgtgtcaaac cagttgcttt gatctgacca aaatcgagtt tggaaggggc aagaaatgag
    57901 agaccacctt ctactacgat cttcgtagca ggtgcaaaag gcggggcatc atgataaaca
    57961 actggcgtat cacttggatt gtgattccca atttcatcgt tggtacttgg cttgttctgc
    58021 acacccttag cagtagcttc tcctgcttgg tcgtttaata caatttctaa tacatctcct
    58081 gcaaaaagtt gattttctgg gatagaaaga acaaattccc ctgtatcatc aatagttgtt
    58141 tctgacaaaa cagcttgatt ttttttcaat gaaaccaagg ctcctgcttc acctgtacct
    58201 gtgatcgtgc gagaagctgc attgatcaat gtggtactta aattagcagg ttcaggagga
    58261 atcacatcga ctgtcgtttt ggcagagtcc agttctgtca cttcttggcc acctcttact
    58321 tttttagcag caacaacttg cacttgatcc tccgttttta atctctcatc attgggtact
    58381 tgaacttcga acaagccgcc ttgttcgcct tctccccatg ggttatatcc tgccccttca
    58441 ttttcatctg tttgtccttg cgtgagagca gtaatttcta cagatgattt cgtcgtatct
    58501 gcgtaagtaa tacgtaaagt cacttctact tcgcctgtcc aagcatctct tggctttgcc
    58561 tcgacacctt cgggaacaac gacatgccca tagatttttt tatctgcatt cgttggcact
    58621 cttaaggatt caatcatcgg gagctgatta ttggcactga ttcgattata atttttcatt
    58681 ccggcacctg tctgagcaaa ttcatttgtt aaacctgcac tagggcttga tacgaaattg
    58741 cctaagtcgg tcccattgat cgataagtct gtcatatcag aaaaataatc cgggtctttt
    58801 tcaaagttat cgaatgttgc gcctgttttt cgccataaag cgatttctga atgggcgatc
    58861 tttaaagaag aatctccatt tccttggaat acccagccgc ctatgccatt ggtctcgctt
    58921 ccaatccgtt tgttttggaa atcaaaatag gtaggagatt ctaagtccac attcaacaca
    58981 tccccactga tgatgccggt cttatcttcc cctgttttcc cgaccatgac taaataagtt
    59041 ttttcacctg ccgtaaaatt taaatcaata tctccagttg tatctacagt tgctccggaa
    59101 tctgcgcgta tatcgatatt tgatttttct ccagtgactg taaaatcagc tgaacctgga
    59161 ctttctagaa aaccagaagc gtcataaaat tggatcgctt gatttccttg attgagtctg
    59221 ttcacgtaag atttgccatc acctgtattt cttaccctca atgagcctcc attggttact
    59281 gtcagctttt gcgtaccatt ctcaaaacga actgcgctgg aacgcccact ctgcttatcg
    59341 atgtcgataa ctgatccggc tccttctacg atcaaagaaa ggccttttcc tacaaaacgc
    59401 atcccattgt tcccgggttg atcgtcatct ccagccattt tgaccgagat ttgtccacca
    59461 cttgatacat ggatttgact agaatctcct tgcaagcgaa atccgcccgt agttgctgta
    59521 ttgatggtga ttttcccacc attcgataca gtgactttcg atcctgaacc tctcatttgg
    59581 atgattccgc gattgttgtc attggtaata ttgatctttg tatttaccga aaattcgctt
    59641 cccgaaccac tgacttcaaa catgacatta ccaggtagcg tatcgactgc ctctccttca
    59701 tcgctaacgc catctgcccg atcataatca agagagacct ttgaaccgct taagatatgg
    59761 attcctggcg ctgtttttcc actaccagtc tcatctcctg aatctagaaa accgtagaat
    59821 ttccctagat acggtccata aagttgacta ccattgatca cattcaattg atagcttcta
    59881 caaaaatagg ctttacccgg agcggcattc agtccttcct tcactggttg gacatccaac
    59941 acaccggaaa caccatctaa tgtgacagtt gcacgaggag cgtaaatcag tcctaatttg
    60001 gacccatcat acaaatcaag tgtcccggta aattttattt ctcccgtaaa atgatatctg
    60061 cttccccaaa aatcggtcat ataagcaatg ataatggcat ttccatcgat atttggtgtc
    60121 aatccacctg gatgtgcagc tgttc
//
LOCUS       DASHFF010000010        60136 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.010, whole genome shotgun sequence.
ACCESSION   DASHFF010000010 DASHFF010000000
VERSION     DASHFF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.010"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            396..788
                     /locus_tag="U2I75_000722"
     CDS             396..788
                     /locus_tag="U2I75_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1695875.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            816..2156
                     /gene="glnA"
                     /locus_tag="U2I75_000723"
     CDS             816..2156
                     /gene="glnA"
                     /locus_tag="U2I75_000723"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN1695876.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3348..4958
                     /locus_tag="U2I75_000724"
     CDS             3348..4958
                     /locus_tag="U2I75_000724"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN1695877.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5250..6119
                     /locus_tag="U2I75_000725"
     CDS             5250..6119
                     /locus_tag="U2I75_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN1695878.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6284..7555
                     /locus_tag="U2I75_000726"
     CDS             6284..7555
                     /locus_tag="U2I75_000726"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1695879.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7552..8847
                     /gene="rho"
                     /locus_tag="U2I75_000727"
     CDS             7552..8847
                     /gene="rho"
                     /locus_tag="U2I75_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN1695880.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8946..9209
                     /locus_tag="U2I75_000728"
     CDS             8946..9209
                     /locus_tag="U2I75_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN1695881.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9654..10103
                     /locus_tag="U2I75_000729"
     CDS             9654..10103
                     /locus_tag="U2I75_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1695882.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            10160..11578
                     /locus_tag="U2I75_000730"
     CDS             10160..11578
                     /locus_tag="U2I75_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1695883.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11578..12438
                     /locus_tag="U2I75_000731"
     CDS             11578..12438
                     /locus_tag="U2I75_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN1695884.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12612..13385
                     /locus_tag="U2I75_000732"
     CDS             12612..13385
                     /locus_tag="U2I75_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1695885.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13532..13897
                     /locus_tag="U2I75_000733"
     CDS             13532..13897
                     /locus_tag="U2I75_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN1695886.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(14045..15253)
                     /locus_tag="U2I75_000734"
     CDS             complement(14045..15253)
                     /locus_tag="U2I75_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1695887.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15272..15550)
                     /locus_tag="U2I75_000735"
     CDS             complement(15272..15550)
                     /locus_tag="U2I75_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695888.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15704..16399
                     /locus_tag="U2I75_000736"
     CDS             15704..16399
                     /locus_tag="U2I75_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1695889.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16841..17638)
                     /locus_tag="U2I75_000737"
     CDS             complement(16841..17638)
                     /locus_tag="U2I75_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN1695890.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17635..18819)
                     /locus_tag="U2I75_000738"
     CDS             complement(17635..18819)
                     /locus_tag="U2I75_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1695891.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18844..20091)
                     /locus_tag="U2I75_000739"
     CDS             complement(18844..20091)
                     /locus_tag="U2I75_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1695892.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20306..22051
                     /gene="dnaX"
                     /locus_tag="U2I75_000740"
     CDS             20306..22051
                     /gene="dnaX"
                     /locus_tag="U2I75_000740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN1695893.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            22071..22385
                     /locus_tag="U2I75_000741"
     CDS             22071..22385
                     /locus_tag="U2I75_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN1695894.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22410..23006
                     /gene="recR"
                     /locus_tag="U2I75_000742"
     CDS             22410..23006
                     /gene="recR"
                     /locus_tag="U2I75_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN1695895.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            23070..23714
                     /locus_tag="U2I75_000743"
     CDS             23070..23714
                     /locus_tag="U2I75_000743"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN1695896.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23729..24058
                     /locus_tag="U2I75_000744"
     CDS             23729..24058
                     /locus_tag="U2I75_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN1695897.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            24072..25010
                     /gene="holB"
                     /locus_tag="U2I75_000745"
     CDS             24072..25010
                     /gene="holB"
                     /locus_tag="U2I75_000745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN1695898.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            25046..25870
                     /locus_tag="U2I75_000746"
     CDS             25046..25870
                     /locus_tag="U2I75_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN1695899.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25863..26210
                     /locus_tag="U2I75_000747"
     CDS             25863..26210
                     /locus_tag="U2I75_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN1695900.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26279..27151
                     /gene="rsmI"
                     /locus_tag="U2I75_000748"
     CDS             26279..27151
                     /gene="rsmI"
                     /locus_tag="U2I75_000748"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN1695901.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27260..28381
                     /gene="dinB"
                     /locus_tag="U2I75_000749"
     CDS             27260..28381
                     /gene="dinB"
                     /locus_tag="U2I75_000749"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN1695902.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28435..29037)
                     /gene="nrdG"
                     /locus_tag="U2I75_000750"
     CDS             complement(28435..29037)
                     /gene="nrdG"
                     /locus_tag="U2I75_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN1695903.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29352..31541)
                     /gene="nrdD"
                     /locus_tag="U2I75_000751"
     CDS             complement(29352..31541)
                     /gene="nrdD"
                     /locus_tag="U2I75_000751"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN1695904.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31840..32634
                     /locus_tag="U2I75_000752"
     CDS             31840..32634
                     /locus_tag="U2I75_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695905.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32624..34666
                     /locus_tag="U2I75_000753"
     CDS             32624..34666
                     /locus_tag="U2I75_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695906.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34947..35096
                     /locus_tag="U2I75_000754"
     CDS             34947..35096
                     /locus_tag="U2I75_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN1695907.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            35107..36621
                     /gene="dltA"
                     /locus_tag="U2I75_000755"
     CDS             35107..36621
                     /gene="dltA"
                     /locus_tag="U2I75_000755"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN1695908.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36618..37823
                     /gene="dltB"
                     /locus_tag="U2I75_000756"
     CDS             36618..37823
                     /gene="dltB"
                     /locus_tag="U2I75_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN1695909.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37868..38101
                     /gene="dltC"
                     /locus_tag="U2I75_000757"
     CDS             37868..38101
                     /gene="dltC"
                     /locus_tag="U2I75_000757"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN1695910.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            38104..39372
                     /gene="dltD"
                     /locus_tag="U2I75_000758"
     CDS             38104..39372
                     /gene="dltD"
                     /locus_tag="U2I75_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN1695911.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39513..40595
                     /locus_tag="U2I75_000759"
     CDS             39513..40595
                     /locus_tag="U2I75_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN1695912.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40595..41398
                     /locus_tag="U2I75_000760"
     CDS             40595..41398
                     /locus_tag="U2I75_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1695913.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41468..42208
                     /locus_tag="U2I75_000761"
     CDS             41468..42208
                     /locus_tag="U2I75_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1695914.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42219..43223
                     /locus_tag="U2I75_000762"
     CDS             42219..43223
                     /locus_tag="U2I75_000762"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN1695915.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43268..45112)
                     /locus_tag="U2I75_000763"
     CDS             complement(43268..45112)
                     /locus_tag="U2I75_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695916.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45324..45842
                     /locus_tag="U2I75_000764"
     CDS             45324..45842
                     /locus_tag="U2I75_000764"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1695917.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45970..46653
                     /locus_tag="U2I75_000765"
     CDS             45970..46653
                     /locus_tag="U2I75_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN1695918.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46962..47035
                     /locus_tag="U2I75_000766"
     tRNA            46962..47035
                     /locus_tag="U2I75_000766"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46996..46998,aa:Arg,seq:tct)
     gene            47194..47553
                     /locus_tag="U2I75_000767"
     CDS             47194..47553
                     /locus_tag="U2I75_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEN1695919.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47676..49307
                     /locus_tag="U2I75_000768"
     CDS             47676..49307
                     /locus_tag="U2I75_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN1695920.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49369..50010)
                     /locus_tag="U2I75_000769"
     CDS             complement(49369..50010)
                     /locus_tag="U2I75_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1695921.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50196..50272
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50292..50600
                     /gene="rplU"
                     /locus_tag="U2I75_000770"
     CDS             50292..50600
                     /gene="rplU"
                     /locus_tag="U2I75_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN1695922.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50627..50971
                     /locus_tag="U2I75_000771"
     CDS             50627..50971
                     /locus_tag="U2I75_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN1695923.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50984..51277
                     /gene="rpmA"
                     /locus_tag="U2I75_000772"
     CDS             50984..51277
                     /gene="rpmA"
                     /locus_tag="U2I75_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN1695924.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(51370..52518)
                     /locus_tag="U2I75_000773"
     CDS             complement(51370..52518)
                     /locus_tag="U2I75_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1695925.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(52530..52841)
                     /locus_tag="U2I75_000774"
     CDS             complement(52530..52841)
                     /locus_tag="U2I75_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695926.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(52894..53289)
                     /locus_tag="U2I75_000775"
     CDS             complement(52894..53289)
                     /locus_tag="U2I75_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1695927.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(53325..53669)
                     /locus_tag="U2I75_000776"
     CDS             complement(53325..53669)
                     /locus_tag="U2I75_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1695928.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            53970..54191
                     /locus_tag="U2I75_000777"
     CDS             53970..54191
                     /locus_tag="U2I75_000777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695929.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            54318..55094
                     /locus_tag="U2I75_000778"
     CDS             54318..55094
                     /locus_tag="U2I75_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN1695930.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            55097..55285
                     /locus_tag="U2I75_000779"
     CDS             55097..55285
                     /locus_tag="U2I75_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1695931.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            55287..55571
                     /locus_tag="U2I75_000780"
     CDS             55287..55571
                     /locus_tag="U2I75_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695932.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            55592..55780
                     /locus_tag="U2I75_000781"
     CDS             55592..55780
                     /locus_tag="U2I75_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695933.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            56106..56345
                     /locus_tag="U2I75_000782"
     CDS             56106..56345
                     /locus_tag="U2I75_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695934.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            56351..57037
                     /locus_tag="U2I75_000783"
     CDS             56351..57037
                     /locus_tag="U2I75_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695935.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            57040..57870
                     /locus_tag="U2I75_000784"
     CDS             57040..57870
                     /locus_tag="U2I75_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEN1695936.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            57887..58738
                     /locus_tag="U2I75_000785"
     CDS             57887..58738
                     /locus_tag="U2I75_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1695937.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            58735..59094
                     /locus_tag="U2I75_000786"
     CDS             58735..59094
                     /locus_tag="U2I75_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEN1695938.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            59109..59270
                     /locus_tag="U2I75_000787"
     CDS             59109..59270
                     /locus_tag="U2I75_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1695939.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            59267..59572
                     /locus_tag="U2I75_000788"
     CDS             59267..59572
                     /locus_tag="U2I75_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695940.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            59572..59928
                     /locus_tag="U2I75_000789"
     CDS             59572..59928
                     /locus_tag="U2I75_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEN1695941.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            59915..60133
                     /locus_tag="U2I75_000790"
     CDS             59915..60133
                     /locus_tag="U2I75_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695942.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
BASE COUNT        19582 a        10274 c        12371 g        17909 t
ORIGIN      
        1 cttcgcaacg atagcaaccg gagtttcttg atgaaaatat gcttctatca ttacaagctc
       61 gtttggtgta agatgggtat aagtcattta tgttcactct ccttgtatgc tttagcgggt
      121 attacaattt gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg
      181 gcgcaataaa aacactaata aaggatagta cagtataata aaaaataaaa ggtatctggt
      241 acgaaaatca actagatacc ttttttgtgt tcttattttt ttgttatttt ataaaaaata
      301 gagatctcat gttagaaaac ctaacatatg cagttgacaa tatttttttt aattggtaca
      361 ctttagccaa cttataaaac gaaaggggac gtctgatggg agaaaaggaa ttgcgccgat
      421 ctatgtccgt atttccaatt ggtacagtta tgaagctgac tgatttgtct gctcggcaaa
      481 ttcgctatta tgaagaacaa gatttagttc atccggaacg aagcgaagga aaccgacgta
      541 tgtattcgct gaatgatatt gatatgttgc ttgagattaa agactatctt tcagagggat
      601 tgaatatggc tgggatcaaa cgtgtatacg aaatggcaca ggcgaaaaaa caagaagcaa
      661 aaaacaagaa gccgttgaca gatcatgacg ttcgacaaat ttttcgtgat gagattttgg
      721 cgcaaggtgg gttaagcaaa acaggtcatt atcagtcgca aggattgcaa agacaattct
      781 ttgactgaat aaattaaaaa aggaagaggg ataccatggt aaagaaacag ataacaggtg
      841 aagaaatcaa acgaatcatt gaggaagaaa acgtacgatt tttacgattg atgttcacag
      901 acatcttagg tacgatcaaa aatgtagaag ttccagtgag ccagatcgac aaagtattag
      961 aaaataaaat gatgtttgac ggttcttcga ttgaaggatt tgttcgaatt gaagaaagcg
     1021 atatgtattt gtatccagat ctctccactt ggatgatctt cccgtgggaa agtgctcatg
     1081 gaaaagttgc tcgtttaatt tgtgatatct ataatccgga tgggacacca tttgccggcg
     1141 atcctcgcgg gaatttgaaa cgagcattga aagatatgcg cgaccttggt tttacctctt
     1201 tcaatttagg acctgaacca gaattcttct tatttaaatt agacgaagac ggtgggatca
     1261 caacgacctt gaatgataaa ggtggttatt ttgattttgc accaacagat ttaggtgaaa
     1321 attgccgccg tgacatcgtg ttggaattag aaagtctagg ctttgaagtg gaagcttcac
     1381 atcatgaagt agcgccagga cagcatgaaa ttgactttaa atatgcagat gtggtagatg
     1441 cttgtgataa tatccaaacc ttcaagctcg ttgtcaaaac gattgcacgc aagcatggac
     1501 ttcacgcgac tttcatgccg aaaccgttat ttgggatcaa tggttctggt atgcattgca
     1561 atatgtcttt gttcaatgat gaaggaaatg ttttctatga taaagacggt gaattggaac
     1621 taagtgaaac agcttatcat ttcttaggtg gtttgctgaa acatgctcgt gcctacacag
     1681 ccgtttgcaa tccaacagtc aattcttata aacgtctcgt tccaggttat gaagcgcctg
     1741 tttatgtcgc atggagtggc cgtaatcgtt cgccattagt acgtgtacca gaatctcgcg
     1801 gtttatccac acgtttggaa ttgcggtcag ttgatccatc tgcaaaccca tacttggcaa
     1861 tggcagtatt gttgcaagca ggactagacg gtatccgtaa tcagatcaca ccgccagcag
     1921 ctgttgaccg taacatttat gtaatggatg aggaagaaag agaagctgca catatccagg
     1981 acttaccttc aactatccat aatgctatca aagaattgcg taaggataat gtaatgattg
     2041 acgcattagg acaacatatc ttctcaaatt ttgttgaagc aaaacgtctc gaatgggcgg
     2101 cattcagaca aactgtttcc gagtgggaaa gagaacaata tctagaactt tattaaaaca
     2161 ggaataccgt gaattagagg ctgtgaaatc agcgttttga cctgagtatt agagaaacta
     2221 acggaaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
     2281 tgaacaccgt ttagtggaga ggttgtgaaa aagctgccct gcaacaagta ataagaatag
     2341 ccaagcaaaa acagcttttc ttgtttttga attggctgaa cttattaccg cagatgcaag
     2401 cttttgaaca ccgtttacca agaggttttg aaaaagacgt ttagtttcga gtattaagaa
     2461 aaattttgtg aaaatggctt tctacatttt tgcataaatt tggcttaata ccgagaaact
     2521 ggcttttgaa taccgtttat ccagaggttg tgagcctacc atttaactcc gaatcacaag
     2581 aaataatttc aaaaacagct tctcatgttt gatggatatt gtgacttgtg accgaggagt
     2641 tggcaggaga acaccgttta tttagaaata aggaggcacg acttttgtcg catctcctta
     2701 ttttttactt atgtgattcg ttaccttcaa tgaatcccta caaggcactt ttgttgaaaa
     2761 aaaggtcatc tttgaaaaaa ttagtggaat tgttttgttt ttgatattat ttaatagaaa
     2821 agatatgcca aaaatccgag aactgtggac tacgtttttt acgtaaagga ggtaaataag
     2881 ggtgaaagaa aaagattgtt tctacgataa gctatataaa ctgttgctgc aagtaggaaa
     2941 aagtagttct ttgttaggtg ggatattatc tatcataatg tttgttttat ttttttatct
     3001 attatttcac ttaaaatttt tcagtaacga ttttctgatt tgcttaggtt taacgttagc
     3061 tatgtttata tcaagcttat tatgtgggtt aaattttttt tgcactcata aaggacttaa
     3121 gaaataattt ctcaaaggat atcgaaaaag aaaaaattgt aagcggatag tattcatttg
     3181 agtacttgac tttcccttag tcagcttgta ttatacacct tgggcaccct tttagagggt
     3241 cagaagttta cagaatttcg gctccctatt cttattgaag aatagggagc tttgtttatt
     3301 gtctgagact tttcctgcac ataaattaaa aaggagtttt tgaacttatg acaaaatata
     3361 tttttgtgac cggcggtgtc gtttcatcaa tcggaaaagg gattgttgca gcttctttag
     3421 gacgcttatt gaaaaaccgc ggcttaaaag taactatcca aaaatttgat ccatacatca
     3481 acgtggatcc ggggacgatg agcccatatc agcacggaga agtattcgta acagatgatg
     3541 gtgctgaaac agatttggat ctgggccact acgaacgttt tattgacatc aatttgaaca
     3601 aatactccaa cgtgacgaca ggaaaaatct attctgaagt tttgcgtaaa gaacgtaagg
     3661 gcgaatactt gggagcaact gttcaagtta ttcctcatat tacaaatgag atcaaagaaa
     3721 aaatcatgcg agcagctaaa atgactgatt cagatgtgat cattacagaa gtagggggaa
     3781 cagtagggga tatagaatct ttgccattcc ttgaagcact tcgtcaaatg aaagcagatg
     3841 tcggtacaga taatgttatg tatatccata caacattgat tccttacttg aaagctgctg
     3901 gagaaatgaa aactaaacca acgcagcaca gtgtaaaaga gctacgtggt ttaggtatcc
     3961 aaccgaatat tttggtggta cgtacagagc aacctgtttc tcaaagcgtg aaaaacaaat
     4021 tggcacaatt ttgtgatgtc gaaccagaag cagttatcga gtcgccagat gtagatacac
     4081 tttactccat cccattagtt ttacaagcac aaggaatgga tcaaattgtt tgcgaccatt
     4141 taaaactaga tacaccagaa gcagacatga cagaatggaa agaattagaa gaacgtgtct
     4201 tgaacttgaa gaaaaaagta cggattgcgc ttgtcggtaa atatgtcgag ttaccagatg
     4261 catatatctc agtagttgaa gcattgaaac attctggttt cgcctataat tcagacatcg
     4321 agatcgattg gatcaaagca caagaattaa cgagagaaaa tgtagaagaa cgtttgaaag
     4381 acgcagatgg aatcttggtt cctggtggat ttggtgatcg cggagtagaa gggaaaatcg
     4441 aagcgattcg ttatgctcgc gaaaatgacg ttccgttttt aggtatttgt cttggtatgc
     4501 agatggcttg tgtcgaattt gcacgtaacg tcgttggatt agaagatgcg tcatcagctg
     4561 aaacgaatcc agatacagca aacaatatta ttgatttaat ggctgatcag gaaaacatcg
     4621 agaatttggg tggaacttta cgccttggat tatacccatg taaaatcaaa aaaggaacga
     4681 agacggctga agcatatgat ggagccgatg ttgtgcaaga acgtcaccgt caccgctatg
     4741 agttcaataa caaatatcgt caattgtttg aagaacaagg gctagtcttt tcaggtgtct
     4801 caccagataa ccgtttagta gaaatcgtag agctgccaga gaaaaaattc tttgtgggat
     4861 gccaattcca cccagaattg atttctcgtc caaatcgccc gcaaaaatta atcaaagcat
     4921 ttgtcggagc atcattagca gatcacgaaa caaaataatt tttgaacttg atagaaagag
     4981 aaaagtagca agagaagggg ataagcaatt ttcttgtcac ttttctcttt ttctaatttg
     5041 ttattatttg taggaaaaaa gatagaaagc aaaagacttt tttcgattaa agcgctattg
     5101 gatattttat tttttgcaaa aaacttcttg aaaattttca ttttcattga ttttagtaga
     5161 aaactaccga aatgtttggt aaaatagggt cgttggttaa gaattgtctt aacaaattta
     5221 gttttacaaa acttaggagg aattttatta tgccagtagt atcaggagct gaatttttaa
     5281 aagctgctcg taaaggcggt tacgctgtcg gcggattcaa cacaaacaac ttggaatgga
     5341 ctcaagcaat ccttgaagcg gctgaagcaa aacaagcacc agtacttatc caaacatcaa
     5401 tgggtgcagc taaatacatg ggtggttaca aaatctgtaa agatttaatc actgatttag
     5461 ttgaagcaat gaacatcact gttccagttg ctatccactt agaccatggt gattacgaag
     5521 ctgctttgga atgtatcgaa ttaggctata cttcagtaat gttcgatggt tcacatctac
     5581 catttgaaga aaacttgaaa ttagctaaag acgttgttga aaaagcacat gcaaaaggta
     5641 tctcagttga atgtgaagtt ggttcaatcg gcggagaaga agacggaatc atcggtaacg
     5701 gcgaattagc tgatattgaa gaatgcaaac aaatggttgc tacaggtatc gactacttag
     5761 cttgcggtat cggtaacatc catggtcaat atccagaaaa ctggaaagga ttagcttttg
     5821 accacttaca agcaattgct gatgctgtag gtccagacat gcctttagta ttacatggcg
     5881 gatcaggtat tcctcaagat caaatcgaaa aagctatctc aatgggtatc tcaaaagtaa
     5941 atgttaacac tgaattccaa ttatcatttg ctgcagctac tcgtaaatat atcgaagctg
     6001 gtaaagataa agaaggtaaa ggattcgacc ctcgtaaatt attagctcca ggtaaagcag
     6061 caatcgttga agatgctaaa tctcatatcg actggttcgg ttcagctaac aaagcgtaag
     6121 catccacata acagatacga tatataggaa tggagaacga cttttgaagt cgctctccat
     6181 tcttttttac ttaaaacggc agaccatgct cgtttctttt ttttatctat gataaaatac
     6241 aagcatatcc atgaaaaaag attaataaac aaggtggaaa cagatgaaaa aaatcgtaat
     6301 caaaggaaac cgacctttag ctggtgaagt aacgatcagc ggtgcaaaaa atagtgcagt
     6361 cgcattgatt ccagctgcaa tcttagctga ttccccagtg atcttggaag gtgtgccgga
     6421 catacaagat gttcattcat taattgaaat tttagagatc atgggcgcaa ctgtccattt
     6481 ttccaacaac atactagaaa tcgatcctcg agaagtcgta tcgattccga tgccgcacgg
     6541 aaaaatcaat agtttgcgtg cttcttatta ttttatggga acactgcttg gtaaatttgg
     6601 cgaagcggta gtaggcctgc caggtggctg ttatttaggc cctagaccga ttgacctaca
     6661 cgtcaaagga tttgaagctc taggtgctga agtgacaaat gaacatggtg ccatgtattt
     6721 acggacagaa aataaaggat tgcgtggcaa tcggattttc atggacgtgg tttcagtcgg
     6781 agcaacgatc aatgtgatgt tagctgctgt aaaagcaaaa ggacagacag taattgaaaa
     6841 cgctgcacga gaaccggaaa tcatagatgt ggcaacttta ctgaataata tgggggcaaa
     6901 agttcgagga gcgggtacag atgtgatccg tatcgaagga gtcgaaacat tacatggctg
     6961 tcggcatttc atgattcctg accgcatcga agcaggaacc tatctagcat tagcagctgc
     7021 agtaggaaat ggtatcaaag tgaaaaatgt gatctttgaa catttggaaa gctttatagc
     7081 aaaattgcag gaaattggtg tccatatgaa aatcagcgaa gatgaaatcg aagtttaccc
     7141 ctcgaaagaa ttaaaacccg caaacatcat gacctatcct tatccaggat ttgccacaga
     7201 tctgcagcag ccacttactg cgttactatt gatgactaca ggcacttcag aaatcatcga
     7261 tacgatctat gcgaaacgtg tgaaccatgt gccggaacta gcacgaatgg gcgctgatat
     7321 taccgtggaa ggaaatatga tcatcgtgaa tggtccaaat aaacttcacg gcacagaagt
     7381 cgtggcttct gatcttcggg caggtgcttg tttagtgatt gctggactat tggcagaagg
     7441 gacaacgacg atttacaatg tagattatat cttacgcggt tatgatcata ttatcgagaa
     7501 actaacagcc ttaggggcat caattgaaat gatagaggaa gtgggagctg aatgagcgat
     7561 tatttgacaa tggcagaact agagaacaaa acgttaaaag aagtttatag ttatgccaaa
     7621 gaatttaaaa ttccttatta cagtaagatg aataaaaagg aattatctct agctgtgatt
     7681 cgtgcacaag cagagaaaca aggattttat tatatggaag gaatattgga tatcgttggt
     7741 caagatggtt acggtttttt acggccaatc aattatggac caagtgcaga agatatctat
     7801 atttctgcct cacaaatcag acgattcagt ttgagaaatg gggacaaagt ggcaggaaaa
     7861 gcccgtccac ctaaagaatc ggaacgttat tatggtttga tgcacgtgga aagcgttaat
     7921 ggaaaagatc cggaagaagc aaaagaacgt ccgcattttc cagccttaac acctctttat
     7981 ccgcaaagac agcttcaact ggaaacaaca ccgcaaagac tatctacccg aatgatcgat
     8041 atcttttctc caatcggttt tggtcagcgt ggattgatcg tcgcaccgcc aaaagcaggg
     8101 aaaacttctg tattaaaaga aatcgcaaat ggtatcaccg aaaactatcc ggaagtagaa
     8161 ttaatcgttc tactgattga tgaacgaccg gaagaagtaa cggatctaga acgaagcgtg
     8221 aaaggcgacg tggtatcttc tacatttgat ctgcagccgc aaaaccatac tcgagtttct
     8281 gaattagtat tggaacgcgc aatgcgttta gtcgaagata aacgagatgt cgttatcttg
     8341 atggatagta ttactagact tgctcgggcc tataacttag tcgttccacc aagcggacgt
     8401 acattgagtg gcgggattga cccagccgca ttgtataaac ctaagaaatt tttcggggca
     8461 gcaagaaaca tcgaagaagg tggcagtttg acgattttag cgaccgcttt agtcgataca
     8521 ggaagccgta tggatgacgt gatctatgaa gaattcaaag gaactggaaa tctagaatta
     8581 caactttcac gtgaattatc tgaacgtcgg atcttcccag ccatcgatat caaaaaatca
     8641 ggtacccgaa aagaagaatt attgatgcca agtgaagaat tagaagaaat ttggaaactt
     8701 cgaaaaaata tgactggaga ttcattagaa tatacggaac aactgatccg ctttttgcgt
     8761 aaaacaaaaa acaatcagca atttttcaaa gaatttcaag atgtctcctt tggtaaaaaa
     8821 aatccgacca gaaatctgaa aagataattg caaccttgac tgaaacatgt taagatgtta
     8881 atgttgtaaa tcgacaaatc aactctggtt cagcaaatgg agcagggcaa aggagcgaaa
     8941 aaatcatgaa agaaaacatc catccagatt accacccagt agtattcatg gattcaacaa
     9001 ctggtttcaa attcttgtca ggttcaacaa aaacttcaca agaaacagtt gaatgggaag
     9061 acggtaatac atacccagtc atccgtgtcg aagtaacttc tgactcacat ccattctaca
     9121 ctggacgtca aaaattcaca caagcagacg gacgtgtgga ccgtttcaac aaaaaatacg
     9181 gtctcaaaga cgcaaacgct gcggaataat aaaaacgctg ataactcaac gtttagcaga
     9241 ctatctgata gggatagtct gtttttttat gttttttgcc aatttaaaac ttcgtttgat
     9301 gctgaaaaag tcgtaggatc ataatgcaca aaacaatgaa aaactagtat atagttttaa
     9361 ttatccactt gataaaaaca ccttatatta acgaaatgaa gaggggttat ttcgaagtaa
     9421 aaaaccattt acctaaaatt agaagctaag ctttcctttc aaggaaggct ttttgttttg
     9481 ttatagactt gcgattgttt gtgagctaga tcgttcattt taatgactta tacacgcgga
     9541 tgatgggtct ttttcctctg ttcattcata atattgaacg gtacagctag cgatgctata
     9601 ctgacctcag ttccaagaaa acgttaacat tttttgtggg ggagaggttt tttatgaggt
     9661 ttgatcaatt gatcgcagaa gaactgattg ttttcgatcc atcgatagat tcaaaaggat
     9721 cattatttga gcgaataggg gatgtgttga aaaaaacaaa taaggtaaga aatgttcaaa
     9781 aagtcgttaa ggcactattc aaacgagagg aggagatctc cacaggaata gaagatggat
     9841 ttgggattcc ccatgcaaaa agcaaatatg tgacaaaacc agcactgtgt ttttttcata
     9901 cagccaaatt aacggactat agcgggatag atgaaatgcc aattgaatat atttttgcca
     9961 tcgtagttcc tgaaaaatca gcagatatgc atctagagat tctcagcaca ctctccagac
    10021 gattgatgaa tgcagatttt agaaagggaa taaaagaagc cactacgcca gcagatatca
    10081 tcagacagat tagtcaagaa tgaaaatagt agagtctttg tccgagtatc gatagtgaag
    10141 aaaaaggagg aaaataatca tgaaaatcgt tggtgtaact gcttgcccga ctggaattgc
    10201 tcatacttat atgtcggcag aaaaattaga agtaacagca agaaatctaa accatcaagc
    10261 aaaattcgaa acacaaggtg taaaaacaga aaatcagtta tctgaacaag aaatcaaaga
    10321 ggcagatgca atcattcttg cagtagataa agaaatcgaa ttggaccgat ttgccgggaa
    10381 aaaagtaaag cgtgtctcta caagccgagc aatcaaagaa ccgcaagtag taatagacga
    10441 agcattgaga gatattggcg tattcgttgt gtctaatgaa aaagaaccag ctgcaaatga
    10501 gaaaaagccg acaatctata atcatttcat gaatggtgtg aattacatgc ttccatttgt
    10561 gatcgcaggc ggaatcatca tcgcactgag ttttgccttt gggattacag cggctgatcc
    10621 caactcagca gactataatg tgttagcagc tgcattttca aggatcggag gagatacagc
    10681 gtttgccatg atggttcctg cgttaggcgc ggggattgct acatcgattg ctggaaaagc
    10741 aggttttgcc ccaggaattg ttgccggatt actggcctct acaggtggtt caggttttct
    10801 tggcggaatg attggtggcg tgctagcagg gtatatctgt gattttctgg caaataaaat
    10861 tagcgtaaaa aaagaatttt ctgctattta tcagctgatt gtcgtcccct ttttatcgat
    10921 cttaattatt gggcttttga tggtatttgt gattgaacaa ccgatcacat gggtgttgga
    10981 tgctttgaca aattggctga atagcttggg aaatacatcc gggctattgt tcggattact
    11041 tatcgggatc atgatggcag ctgatatggg tgggccgatc aataaatcga tttcgacttt
    11101 ctcgatcggt ttgatgtcag ctggagtaaa tgcaccgatt gctgcttgta tggctgcagg
    11161 aatgacacct cccctaggta ttgctttagc cactttatta ttcaaacata aatttaccaa
    11221 agaagaacgg acttcaggtc aatcatgctg gattttaggc gcttcgtata ttacagaagg
    11281 tgctataccg tttgcggtat ctgacccgat ccgcgtgata cctagtttga tgacaggttc
    11341 agcagtcgct gcagggattt ccatggcagt aggagttact tcaatggcac ctcatggagg
    11401 gatttgggtg atgctaattc caaatgttat gaacaattta cctatgtatg tcttggcaat
    11461 tgcatcaggt acagtcacta cagcctttat gcttggacta ttgaaaaaaa cagtgaaaga
    11521 gaaaaaacaa gacaagtgga cgaaaaatga aaatctaaca aatgaggtgg aagcgtaatg
    11581 ttatatacaa tgaaagagtt attgaaagtg gctaaagaga atcaatttgc agtaccagcc
    11641 ttcaatattt gcagctatga tatgatgaaa gcaatcatcg aagaagcaga gagattagaa
    11701 gcaccagtaa tcttagaaat tcatcctgat gaaatcgcct atttaagtga tgagtttgtt
    11761 gcggcagtaa gagcctatgc ttatacaagt aaactgccga tcgttatcca cttggatcat
    11821 ggcggtacga tagaagatgt cttacgggcg attcgaaacg gctattcttc cgtgatgatc
    11881 gatgcgtctt tgaaatcatt cgacgacaat gtacagatta cgaaaaaagt ggttgcgtta
    11941 gctcatgcgg taaatgtctc agtggaggct gagcttggaa cgattgggaa taatggttct
    12001 gctgaaggag gtgctgaaac aatcatttat acagatcctg atcaagcgaa gatttttgtg
    12061 aaagaaacag gaatcgatac acttgctgta gcaattggaa cagcgcatgg cttgtatccg
    12121 aaaaacagaa ctccaaaatt aaatatcgac ttactgaaag aattgaacca acaaatcgat
    12181 cttccatttg ttttacatgg cggttcaggt aatccggacg cagaagtcag cgaagcagta
    12241 aaatacggag ttgccaaagt gaatttgagc tcagatctta aaagcgtctt tttcaatgct
    12301 ttgagagaaa tattgagtga aaatccatcg atgtacgagc cgaatgcaat ttatccagca
    12361 gctaataaaa aggtacaaga agtagtcaaa cataaaatgg aaattttgaa cacagtaggc
    12421 aaggctagtt tatactaaaa ataaaaatgt gtggtcaaga gggtatgaca atagtcttgt
    12481 cataccctct taaccacaca cgatgttcag aagtagtttt ttcgaaaaat ttcttcttat
    12541 ttctcgaagc caatcacttc tgtcacaacc tcttttttcc tgttactata taaagtaaat
    12601 ggggtgattt tatgttgaat gaagtggaga atagacagca ggagattgtg aattatctaa
    12661 aaaacaacca atttgcaaga gtcaccgatt tgatcgagtt agtaaattat agcgaagcca
    12721 cgattaaacg tgaccttgtt tttctggaaa aaagtggatt gatccgtaga gttcgcggtg
    12781 gcgccatgct tattgacaat cgaaaaattg acgtgcctta cctgatgaag atcaccaaat
    12841 tagaagaaga aacagaaaaa cgtaagattg cagatacagc ttcttctttg attcgagatg
    12901 atatggtctt attcttggat tccagtacaa cctctttaca cttagtcaga aacctagcaa
    12961 gatttgatgg tttgcagatc attacgaatg gcatcgtcac agctgctatg ttgagtgaat
    13021 atacaacggc aaaagtatct gtattgggag gggcaattgt tccgaaacga gcaacaatca
    13081 acggttcaaa agcctataac gatgcattgt catattatgc ggatttagcg ataatgagtt
    13141 gtcgcgggct agatttcagc caaggagtaa cagaaactca tgaaggagag gcaatggtca
    13201 aaaaagcttt tagaaaacaa tcagaccggc ttgccgtttt agttacacag caaaaaattg
    13261 cacataaatt catgcatcaa gccattgctt ttcatgaaat cgattatctg gtgacaagta
    13321 aagaactgac aaaagaacag tatgcgaaag taaaggaaca ccatatcgaa tgtctgtacg
    13381 gataaattat tacgtgaatg ttctaagtgg cttcctcatt tttagatagg accttatctt
    13441 tgctcgcttc tctcagccga tggtataata atgaaaaggt tatcttgtta gataatctta
    13501 cttttttatc caggagaaag gagtgcagaa aatgaaacat gtgaaggcgt tggtagtgaa
    13561 ggcaatcatg atctgggcga ttctgtgggt cgtgctgaca ggtttatacg gtgttagctt
    13621 tatggattca acgattgttg gggtaattat tgtagtcatg atctatgtgc taggggatct
    13681 gttgatctta cgtaaagtag ggaacatcgc agcgacgatc gcagatgctg gttcagctgc
    13741 agttatcttg tggctctatc tatattttat gaatgataca gtcgatattt ggatgaaagt
    13801 attgattcct gctctattga tcggtgtggc agaatggttc ttccacaagt ggttgctaag
    13861 tcaaggcatc gtaccagatg aacggaaaat ggaataaatc aacagttaaa ctattcttat
    13921 cgataaatga aagctttaaa aataaataga tgtttaatta agaataatca taaactattt
    13981 ttactcaaaa aaccgctcag attattttca gatctgaacg gtttttttta tctattcatc
    14041 aagatcaaat ttcatttttc aatgattcaa tcaattcgcc ctctgcttca tgactatgtt
    14101 ttcggccatt aacaagatag agtgctgtac atatcaatgg aatgactccg gcaatcacat
    14161 acaatcctct aaatgaagta agagatttta gcatccccat caaataagga ccaacgccta
    14221 agcctaggtc taaaccaata aagtaggtag ataaagcaat actgattcga tgttcgcgga
    14281 cgatttttaa acaaacagcc tgtccatttg acataaacgt accataacct aggccgacta
    14341 atgctccaga gataagcaat gtcacactat tcgtcgttac actcagtaaa aataatcctg
    14401 ctgttaagaa taaatagcta ggatacatca cgtattgttc tcctttgaca tcgaaaatcc
    14461 ttccagtcaa tggacgtgta aatgtgatga ccaaagcata tacgacgaag aaaaatgacc
    14521 cagcagcaac taagtggatt acttttgtat aagaagataa aaatgataaa acacttgaat
    14581 aagaaagacc catcaaaaag gcaattcctg taatgaaaag tgctttcttt tcaataaaac
    14641 tattgatatt ccatgagcga agagatattt tttgttcttc agataatgga atatttttta
    14701 caggaaagac aaggcaggca atcgtaacaa agaagaccag tacaattgaa aaccagataa
    14761 tgaaacgaaa atcagtcata ttcagcaaaa tcattcctaa aaatggccca acagcggcag
    14821 ctaaactagt acttaatcca taataattga tgccttcccc ttttttagat gcagggatat
    14881 atgcagtaac gatagcattt gtagctgtag aaactgtacc gtagccgaat ccattcaaga
    14941 atcgaactaa atacaatgcc ccgatagttg gcgtataaag atatgctaat gttgaaagga
    15001 gatagaagat cgcgccgcct cttaaggtaa acttccgacc aaatagttct aactgtttcc
    15061 ccatgaataa gcgtgccagc aatgttccga tgatatagat cccagaagca aaacctgctt
    15121 ggctcaagct ggcgtgtaag gtgtcttgcg caatgactgc catgatgacc atcaacagat
    15181 aataaatcag gtagacaata aaattgatca atgtgattcc aataaaatat ttattaaata
    15241 atttcttttc catgtatgtt atacattccc cttaagctga atatgctttt aatttttgta
    15301 tttgaacttc tacatcgtga taattggatg gatagcgatc aacaagttgg cgaataaata
    15361 aatctcttgt agacataaac agatttaaaa attcttcgca ttcgtttgtt ggattctcat
    15421 ccactgcttt gatcaattcg atcgagtttt gatacgcatc atttaaaaag actaaagaag
    15481 ctttcatatc aaatcttgtt tgatatgctg tttctaatcg attaatgatt gtgttcatcc
    15541 agtaattcat gttttttccc tctattctta aaaaatttta cttctgcagt ataacgaata
    15601 aaaaaaagga aaaaaatcgc ataagtgatg gaaaaaggta cttaacaaat gttaatatca
    15661 ttagtaaaat acctttggac tttaaaaagt tttaaagtac tgtatgatct ttttagatga
    15721 aagtttggat gggggagcat cattggatag tagagtctta aaggaatatt tggataaaaa
    15781 tgattttccg attatacgca aaggatatca taaatatttg acattcgaag ggatggaaga
    15841 ttcccatacg tatatcttga aagagggaac ggtaaaagca agtgttattt caaatgatgg
    15901 gagagaattt aaccttcgct atatcaaaga tcttgaaatc gtctctcttt taaaagacga
    15961 atattcacaa ttcatcgatt cgccgtataa tattcgagtt gaatcagaat atgcagagtt
    16021 ttatcgaatc aatcgtgtgc gcttttggca aaatatcaac caagacccaa agctacaaca
    16081 ctatgtaaaa gagtattacc gctatcgtct gctttattcg atgaaaaaaa tgcagcaaat
    16141 gctattaaac ggaaagctgg gagctgtttg tacgcaactt tacgaattat atgatttatt
    16201 cggtgtgcgg acagaagaag ggatgctgat cgatttttcg atcacgaatg aagaaatcgc
    16261 caaattttgt ggcatctcaa ctgctagcag tgtaaacaga attttaaaac aactgaaaaa
    16321 agatggtatt atcaccgtta aagaccatca tatcgttatt ttgaatatgg acttgttaga
    16381 agacaatatt atcttttgat gaacaaagag gttgtgaaat cagcgttttg acctgagtat
    16441 tagagaaaca aacggaaaaa tagttcctca tattttgccg ttttttgagc taataccgag
    16501 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta actctgagtc
    16561 acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg
    16621 aggagttggt aggagaacac cgtttaccaa gaggttgtga aaaagctgtt ctgcatcaag
    16681 taataagaag agccaagtaa aaacagtctc ttatgttttt aacttggctg gacttatcac
    16741 cgtagatgta agcttttgaa cgccgtttat ttgcaacaaa aggggctgtg acacgatttt
    16801 tgtcacagcc ctttttttgc gaacattcat ttagcaattt ttagaaactt ctgccgccac
    16861 cgccaaaagt cccgccaccg ctagaatggg tagtacttcc gccaccgccg cttccaggag
    16921 gtgggctttt ggggattctt cttgtcgtga caaaagaatt tgtcagacga tcggtttttt
    16981 cagttaattt gatactggat ttttctcgga atggatattt gtagatgcct gatttcaatt
    17041 gataacgcga aacagtcaca aaataaaaga caagactaag gataagagcc aaaacaacag
    17101 caatgatgat ttctactgtc gtcaaaacct tatagcgtgt aattttgcca gtttcttcgt
    17161 ctacacgata atgtcctcct ggaactccgt cttccacata tgttgctgat ttagacaggt
    17221 aagcttttgc agcattgtaa taggcagcat ttgtcatctg atcataaaca tcatctaaga
    17281 tcgaatcgat tctgctatca gtcaaataat cgatcatatt accagaagtc gaaatgtaga
    17341 tttcacgctg tcccatatct aatagcaata ctgagccatt ttgatcattt ccgactgcgt
    17401 ctcttaagta attatcagca aagtctcttg gttcttctga attagatgta gtcgttacga
    17461 tcaagactcg tcctttgatt ttttcattca gcttttctgc ctgttgttcc aaagattgga
    17521 tttgatctgt tgtgaataac ccagcctcat catttaccgt agctgtatct gcatgagtag
    17581 ccaccggcca gccaacactt actagcaaaa gaaagaataa aaaaaatttt ttcatcatat
    17641 caagtaacct ccgatcagaa agaggatcgc tagcccagcg aaaatcccaa gagcggtcag
    17701 tcccaatttt ttctggctga tcggcaggac accgctgacc tttcctgttt gtccattcat
    17761 tgcgtagtag tagacttttt tacttgagtc attgcttcga taagtaacaa gccataccgg
    17821 cagtaatacg taattattta cttcgtttgt gattgaagca gaagttcgca caccggtcaa
    17881 tgttgtatat ccatttgcgg tatcttttaa cagcttctct gaatagtctt ttaattcaga
    17941 ttgaatctgt gatttgattg cctcatattc gatatctctt ttttctgctt ggaacccggc
    18001 taaatactgg cttttaaaat caattgcttt atctaacggg aaaggttgca cgccttcgac
    18061 catcttttgt tgcgtatttt ttgataaggc attttttact agttctttaa aagaaagctt
    18121 gcctgctctt gaaacagcaa attgcttcgt ttctgtgtat tcgatgtcac ctatccgcca
    18181 tatgcggata actgtaccat tcgcctgcat ggcaccatct acttcagcat ctgtagccca
    18241 ataagggaaa tacacacctg tcaacttatc gatttgatcc ttattgaaaa aagctttggg
    18301 aacaaaccat tttttcttgg tccaagcgag aaatctgttg attgcttctt ctttctcgac
    18361 agcaaaagga agtactttat ttggcaagaa ttctccgctt aaacgtcccg atagtacgac
    18421 gggattatgg cagtagtaac aataagtcgc agcagttgtc gcatcagtca cgatctctgc
    18481 accacagttg ggacaaagaa aaagatctat cgctgcggta ttatctgcag attcgtccgc
    18541 tgtatctgaa aaaccgcctg cctcgtcaac cgctttgcgc tcggcttcat tcatatcggc
    18601 aagctgttct tgggctgtaa aagtaaaatc ttcggtaggt gaaggttctt ctttgctttc
    18661 ttcgccagcc atacgcgctt ctttttgctt ttgctcatat tggctgactt cttcttctgt
    18721 atagatattc aggcagtatt cgcaatgaaa tttttggtct ttgggatcaa aagtcaacgg
    18781 accgccgcaa ttaggacatt tatgtgtaag aacatccatc agctgcacac ctttctaaat
    18841 aaattaatta acggggattc ctttaaacgg tttaccacag tttggacaga atttagggat
    18901 gccgttggaa agatccacga tctcattgca ttcactgcat cgcatttgta gcttaggctg
    18961 aacgttttct acaggtttgg cagctccgca gttggaacag aattttccgg aattttctgt
    19021 cccgcaaact ggacatgtcc aagtatccgc atttccttgt gcagtttgtt gattttgttt
    19081 ttctgcttgt tgtttgattt gttcttggtt attttgagct gcttgagaaa gataagaacc
    19141 atttgcattc attcccatac ctacacccat aaagccggtc attgctcctg cttcattttt
    19201 tccggctgct tccataccac gtgcaatgga accttgcacg tatccttcac gtacgctagg
    19261 atcacctagc atcgcccctt cgttacgcat attgatcagt ttcacagaat catcggtgta
    19321 agaaatgctg gctacagcca cagaaacaat ctccatccca cgtaattcac gccaagaatc
    19381 atccaatgca gtatccatgt atttgcttaa ttccaaactc ttagatggta cataagaaat
    19441 ccgttgacca tctgcagaca tctgattgat tgctgattgt agcgctgtta ggaattcagc
    19501 taaatactgc tcattgatat cattgatttc cacttgtgtt ttgtttttag ggattgcgtt
    19561 tgtataaaat aaaatcgggt caacgacatg aatagaataa gtgccatgtg cccgtaagaa
    19621 taattctgcg ttatagaaat tgtcaaagta gttcaacggc gcgcttgtac cgaatttgat
    19681 tccctttatt tcttggagat tgatataaaa aacttgttgt ttctgcggtg tgacgccgcc
    19741 aaatttgaat cggtcaaaag tttcagcaat tgcttctttc agtgttccat tgaacatgga
    19801 aggagctgcg tcatttttaa tggtgtaata accttcctca gcggtataat cgatgatttt
    19861 tccaccatcg accaaaagca tcatcatgtt tggatagaca tggacgactg ttccgtctgt
    19921 taaaacatct tctgttcctt tacgattgga gccacgtttg tcatctttgc ggactttgac
    19981 accttttgtc atgaccgttg tatcactcat attgtctggt tcgatgactt ctagccattg
    20041 atctgccaga ccgccgccaa ccatacttgt tgctgctttg ataagaccca taaaaaattc
    20101 ctcctctgat tcagctagtg ccaaacaatt attgtttatt tattatacca taggaagatg
    20161 tgaaacttct cggactagag acggaattga aatggaaaat tgcgtattgt ttaaatacct
    20221 gaaaaagccg agaaggatag tagttttttt acctaatatc acgtataata agagatagtg
    20281 aattttttaa aaaaggggga tccttatggc atatcaagca ctttatcgag tctggcgttc
    20341 gcagcgtttc gaagacatcg ttggacaaaa agctgtgaca cagacactaa aaaatgctat
    20401 tgtttcccac aaaacctcac atgcctactt gtttacagga cccagaggaa ccggaaagac
    20461 gagtgcagca aagattttcg caaaagcaat caactgtccg aacagtaaag acggtgagcc
    20521 atgcaacgaa tgcgaaatgt gtcgatcaat tactgctggt acgcaagaag atgtgatcga
    20581 aatcgatgct gccagcaata acggagtcga agaaatccga tttatccgtg atcgggcaaa
    20641 ttatgcgcct acaaaggcag cgtacaaagt gtacattatt gatgaggtac atatgctttc
    20701 aacaggagct ttcaatgcct tgttgaaaac attggaagag ccaaaagaaa gtgtcatatt
    20761 catcttagca acgaccgaac ctcacaaaat accagctacg atcatttcac ggactcagcg
    20821 atttgatttt aaacggatca atacggcaga tattgtcgaa catcttgaat ttatcttgaa
    20881 agaatcgact atcagctatg aaccagctgc attgaatgtg attgcccgtt ctgcagaagg
    20941 cgggatgcgg gatgcattga gtattcttga tcaggcaatc tcgtttagtg aaggaacaat
    21001 tactttagat gatgccatgc aagtgacagg tagcttaact gacgaaatga tggacagtta
    21061 cttgagtgct tgcgtgaaaa aagaagtacc agaagcattg gaaacattga accaaattct
    21121 cgcagccggc aaagaatcac gtcgctttct agaggatatg ttacaatact gccgtgactt
    21181 gctgatgtat cagcaagcac ctaacctagt tgaggaacaa tcgagtacga tcagaagtac
    21241 atttacagaa ttggcacaaa ctgtttcagc ggatcagctg ttttcaatga taaagatttt
    21301 gagcgatact caaaatgaaa ttcgttttac caatagtgcc gctatttatt tggaagtcgc
    21361 tacagtcaaa cttgcacagc taccgtctgc ttctgttcct tcagcagatg tagcatttgc
    21421 agatgatcag atagtaaaac agctacaaca agagattaag gaactaagaa acgaaatcaa
    21481 ggaactacgc agtaatacag gaattgagaa tacaaaagta cagccggtac agcaaacaaa
    21541 tcataaaaaa cagacaagta cctatcgtgt accgacggaa cgagtatata aagttctgcg
    21601 ggaagcgaca agacagcatc tgaaccaagt aaaagaagta tgggaagact tgctgatgag
    21661 tctatctgtc actcagcgag caatgcttaa agcaagcgaa ccagttgcag ctggacctac
    21721 aggactagtg attgcgtttg attatgagat tgtttgtcaa agagcagcaa atgacgaaga
    21781 attgcagctt tcggtacaca acaacttaag ccgaatgatc aaagattatg cgcctgatcc
    21841 tgtttatatt acaagagaaa gctggccaga acttcgaaga aattatttga cgcaggcaca
    21901 agaagaggga acagaaaact cttttgcttc tgaagactca gaaggagaaa tcgagttgct
    21961 tcctcaagaa caagaagtac cagaagaaac acttgtagta aaagcagagg aactgtttgg
    22021 ctccgaagct gttacagtgg tagatgatta ataaaaagga gtagataatt atgatgcgtg
    22081 gaatgggcaa tatgcaaggc atgatgaaac aagtacaaaa aatgcaaaaa gaaatggcag
    22141 aagcacaaga gctgctaaat aaaacagagt ttacaggtaa agccaccaat gaattggtca
    22201 aagcggtttt tacaggcgat cgtcgcatga aagatatcca aatcaatgaa gaagtcgttg
    22261 atcctgatga tatcgagatg cttcaggatc ttgtcgtaat ggcagtcaat gatgcgatcg
    22321 cacaaattga tgtggaaact gagaaaaaat tagggaaata cgcaaaaggt ttgccaggat
    22381 tttagtttta aactgaagga gtaatgaata tgcaatatcc agaaccaatt gcgaaactga
    22441 tcgacagcta catgaaactt ccaggaatcg gtcagaaaac agcgacaaga ctcgcctttt
    22501 atacgatcga tatgaaagat gaagtagtaa atgaatttgc taaatctttg ttaagtgtaa
    22561 aacgcgattt gcatttttgt agtatctgcg gaaacatcac agaagaagat ccctgcgaaa
    22621 tatgtagaaa tccttcacgt gatcgaagcg ttatcctagt ggtcgaagag ccgaaagatg
    22681 taatggcaat ggaaaaaatg cgagaatatc acggacttta tcatgttctc catggtgtgt
    22741 tatcaccgat ggaagggaca ggtccagaag acatcaatat ttcttctctg ttgcagcggc
    22801 tgcatgatgc agatgtaaaa gaagtgatta tcgcaacaaa tgcaacaaca gagggagaag
    22861 caacagctat gtatctctca cgtctgatca aacccgcagg tatcaaagtg actcgtctag
    22921 cacatggtct gtctgtaggc tctgatatcg agtatgcaga tgaagttact ttattgaaag
    22981 ctgtcgaagg aagacgtgag ttataaattt ctaattaagt gttttttcag atacaatata
    23041 aaagcagcac aagagatggg ggcaacaatg tgaacggact atttattaca atcgaggggc
    23101 ctgatggtgc aggaaaaacc agcatcttga atgaacttta ccctcttttg aaagaagtag
    23161 caaaaacaga aatcattcaa acaagagaac caggaggcat tccgattgct gaagagatcc
    23221 gagcagtgat ccttgatcca aaaaatgatc ggatggatga acgtacggaa gcactgcttt
    23281 atgcagcagc aagaagacaa catctagtcg aaaaggttct gcctgctctg gcaaaaggga
    23341 aaatcgtttt atgcgataga tttgtcgaca gttctcttgc ttatcaaggt gcaggccgac
    23401 gcatcggcgt taccgagatt gctcggttga atgagtttgc tacagaaggt actacgccgg
    23461 attttaccct ttatcttgac gtagattcag acaccggatt aagaagaatc aagaaaaaca
    23521 gacaaaatca aatcgaccgt ttagattcgg aaggtttgga attccaccag cgtgtacgtc
    23581 atgcttactt aaagctggca gaagagaatc cggaaagaat ccacaaagtt gatgcaagaa
    23641 aaagctttga agaagtgctg caaacaagtt atcatacaat tattgaacag tatccgcaat
    23701 tttttgaaaa ctaggaaggt gaacgtttat gaaaattatt ttagcgatta tccaagacaa
    23761 agacagcaac cgtttatcaa atgaattgat tgatgcaaat atccgtgcca caaaactatc
    23821 atcgacaggc ggcttcctta aggctggaaa cagtacgttt atcgtgggta ttgaagatga
    23881 ccgtgtagaa gaagcattgg aaatcatcaa aaaaacgtgt gaatcaagaa agcaatttgt
    23941 atctacacct gttacactgg atatttcgat ggatgggggc gtcccttatc cagtagaagt
    24001 agaagtaggc ggtgccacag tcttcgtctt acctgttgaa ggattccatc aattttagga
    24061 ggagctgagc tatgaatcaa gaaataaact tggctcaaat gcaaccgatc gtgtatcagc
    24121 agcttcaacg gagttttgag catgggcgta tcgctcatgc ttatcttttt gaaggggaga
    24181 aaggaacagg aaaacatgaa gtaggtatat ggcttgcaca gcacctgttc tgtacccaaa
    24241 tgaaagacaa tctgccttgt ggaaaatgca acaattgtca gcggatcgaa aagaaagaac
    24301 atccagatgt tttggttatt gaaccggaag gacaaaccat caaagtcgat cagatcagac
    24361 gtttgcagac ggaattcagt cgcagcggtt acgaatcaag aaaaaaagtt tttttgatca
    24421 aagaagctga aaaaatgaat agcagtgcgg caaacagttt gttgaaattc ttggaagagc
    24481 cccctggaga ttttttagcg gtgttagaaa cagatgcagt aggaagaatt ctccctacta
    24541 ttcaatctcg ttgtcaagtt ctgcattttc aagaactttc gaaagaagca ctgatccatc
    24601 gattgcaaga agagcagatt ccattggaaa aagcaaaact tttggctttc ttaacaaaca
    24661 gtttaggaaa agcagttgaa atctctcaag atgaatggtt taatgatgct aaggacttga
    24721 tttatcaatg gttcgtttac ttgcaaaagc aagatactca agcctttatc tatgtccaaa
    24781 aaaaattggt aaaatcattt aaagaaaaaa cacaacagtt tacaggatta tctgtcttga
    24841 tgttttatta tcaggaagca cagcgtcaag cagtagcaaa cggcgaatat ggcaaagtga
    24901 aacagataaa ccagataatt gaacggatat tgatcgcaga gcagaaacta cgcagcaatg
    24961 tcagttttca ggctgttgcg gaacagttcg ttttgcatac gattttttag cttgccgcaa
    25021 aggatgatga tcaaggagga tggaaatggt agaagtagta ggtgtccgat atagagatgc
    25081 aggtcatatc tattattttg caccaggaga ctctgaatat gtttatagtg acaaggtatt
    25141 agtcgaatct cagcaatcct tacatattgc tactgttgtc cttcctaaaa aagagattga
    25201 tcccaatgac cttcctgaag ctatccgtcc gatcttgcat aaagcgtcac aagctgaatt
    25261 agaaaaagac aaaaaaaaca aaaaagatgc ttatgatgca ttgaaaatcg caaaagaaaa
    25321 agtgcagttg cgtgagttgg aaatgaaaat ggtcaaagcc gaatacacat ttgatcgaaa
    25381 taaattgatt ttttctttca cagcggacgg gcgaattgac tttagagaat tggtaaaaga
    25441 tctggccgct gtatttcgta ctcgtattga gcttcgacaa attggtgtcc gcgatgaagc
    25501 aaagataata ggcggaattg gtccatgtgg gcgtccatta tgctgttctt cttttttagg
    25561 cgatttcgtg cctgtatcaa tcaaaatggc aaaagatcaa agtttatctt tgaatccaac
    25621 aaaaatttcc ggtttatgcg gacgcttgat gtgctgtctg caatatgaga gcgaagaata
    25681 cgagacagcg aaaaaagaac tccctgactt tggaaaagta attgctacac ctgatggtaa
    25741 agggaaagtc gtagggttga atctgcttag ccgtatcgtt agtattcggc tgactggacg
    25801 ggaaaatcct gtcgaatatg aatgggatga attgaaagaa tttatccaag taggtgagat
    25861 gaatggataa gagatcatta tacgacggat tgaatcaatt agaatcagag ttgagaaata
    25921 cattaacaga acttgttgaa atgaagcatg ctttgcatga gatcgtagaa aaaaatacaa
    25981 ctttggagat ggaaaatcaa cgtctgcggg aacacctgcg ggaattgaac caagttgcac
    26041 aagctccagc tgaaaatgtg aagcaggaac tttctaagtc tcgtatgaat cttgaaaaaa
    26101 tttatgaaga aggattccat gtctgctacg atttatatgg atcaagaagg gacaatgacg
    26161 agccttgtgc gttttgtcta gaagtgatct atcgcgaaag cggtcgatga ccaataacaa
    26221 gaggagactg gagcggagaa atactgctcc agtcttttta ttaaggagat tgtgacagat
    26281 gtataatcaa aaaagtttca agggaaatcc ggtatatggg gatttatatc ttgtaccgac
    26341 gccaattgga aatttagaag atatgagcta cagaagtgtt cgtatgctgc aagaatccga
    26401 tcttatcgca agtgaggata cgcgtaatac acaaaagtta ttgaatcatt ttgaaatcca
    26461 tacaccccaa aaaagtttac atgaacataa ttataaagaa cgtgtccctc aattgattgc
    26521 tttgttaaaa gaaggaaaaa cgattgctca agtcagtgac gcaggaatgc cttccatcag
    26581 cgatcctgga catgagctag ttttagcctg cataaaagaa gggatttctg tgatctctat
    26641 cccaggacct acagctggga tgacggcttt gattgcttca gggctagtgc ctcaaccatt
    26701 tttattttat ggcttccttc cacgtaagaa aaaagaacag aaagaaacat tgaaaaagct
    26761 aaaagaacag acagttacgc tgatttttta tgagtcacct tatcggatca ctgctacatt
    26821 gacgaatttc ttagaaattt ttggaaatcg ccaagtcgtt ttgtgtcggg aattgacaaa
    26881 ggtccatgaa gaatacctta gaggaagtac ggaagagcta ctcgcttata tcgaagaaca
    26941 tccagtaaag ggtgaatgtt gtctgcttgt agaagggaat ttgttcgaaa tgccagtgac
    27001 ggaagttgat gaagagttgg gaacgttaaa ggaacaagta gaagcaaaga tcgcttcagg
    27061 cgaaaaacca aatgctgcta tcaaagcagt agcgctgaaa aatgggctga aaaaacaaga
    27121 agtctaccgt cagtaccatg aattagactg agatagtata agttgtctat aatgaaagac
    27181 taatgaggaa atcagctact aacttttcaa atctcttgaa cgcgaacgaa tgttcttgta
    27241 taatgaataa gaggtgagga tgatggagct gcaatttcct ttacataatg atacatcaag
    27301 aaaaatcatt catattgata tggatgcttt tttcgcttcg gttgaagaac gagatcatcc
    27361 tgagtttcgc ggacatccat tagtaattgc cagacacccc agtgacactg gtgggcgtgg
    27421 agtagtgact acagcaaatt atgaagcaag aaaattcgga attcattcag caatgagtgc
    27481 acaaaaagcg tatgagcttt gtccgcaagc catctttaag cctggcgacc atcataaata
    27541 cagcgatatt tccaaacaag tacgggaagt attctatcgt tatacagata ttgtggaacc
    27601 agttagtatc gatgaagcgt atcttgatgt gaccgaaaat aaaatcaatt gcaagtcagc
    27661 catcaaaatc gcccgaatga tccaagcaga tatttgggag agtgtccagc taacatgttc
    27721 tgcgggagta agttacaata agtttttggc caaattagca tcagactatc aaaaacctag
    27781 aggaatcacg gtagttgccc ctcaagacgc tgtgccattt ttaaaagctt tgccaattga
    27841 aaaatttcat ggggtaggga agaaaactgt acctagaatg catgagctag ggatttatac
    27901 aggaaaagac ctatatgaat gcacagaaat gatgttgatt cgaaattttg gaaaaatggg
    27961 ctattctctt tatcgtaaag tcagaggaat ccatgattct ccagtaaacg ttacaagaga
    28021 gcgaaaatct gtgggaaaag agcatactta tgggcagccg ttgcaaacgg aagaagcagt
    28081 gctgactcag ttgcgccaac tagctgaaaa agtagaagaa gcactgagaa gagtccaaaa
    28141 acatgggaaa acagtagttt tgaaggtcag gtacacggac tattctactg tgaccaaaag
    28201 agtgacattg ccagaatata tctataaaaa agaagcactc ttttatcaag caagccttat
    28261 atgggaggaa atcttgggag tagaaaaagg aatccgattg ttagggatta ctttgacgaa
    28321 tctggacccg atgacttatg aaaacatcgt ccttccatta tgggaaaatc aagaaatata
    28381 aaaacagctt tgagagttga aaccggaaat ccgttttctc tcccaaagct gtttttatat
    28441 caagtcttgc tttttgattt gttcataggt ttctgttgca tccgtacatt tttcccaaat
    28501 cacaacttga ttggattcta aagatttttg gacgtcaatg atccgctggt tgctacttcc
    28561 gcgaaattgt agttttaggt cacgtttgct taattcaaag cgtccgtcaa caaggatatc
    28621 gatttgcgaa aggagttcta atttatcttc tgaatcttgt agcaattcat caaaggtata
    28681 gcctgtccat gaccagatat cttttgtcgt accgaactct tttcggatac gtcgtacgac
    28741 ttgcagacaa acatctgtat tcaaaaaagg ttcgccacca agtaaagtta atccttgcac
    28801 gtaatcgttg cgcaagtcag cgatgatttg ttcttctaat tcaggtgtga atggtttgcc
    28861 atatcggaaa ttttgtgcag ctttgttgaa gcagccttcg caggcaaata agcagccact
    28921 aacgtaaaga ctgttgcgga caccttcacc atcgacaaaa ttaaacggtt tgtagtcagc
    28981 aatatagttt tggctgtagt cttttgctaa ccattctttt ggttttggat ttctcataaa
    29041 aaaacttctt tcatttaagg ttcaaagata tagctattga agataaaatc tactcgacca
    29101 cataaataag aacaaactga cctaaatcca ttgttcaaga aagcttgttc ttattttgat
    29161 agagggcgtt caaagataca aagccatcga aagagggtgg tgaaaaaaga cttgtcatag
    29221 cctcttattc tgaataaacg gtgttcaaaa gcttgcatct gcagtaataa gcccaactaa
    29281 acgaaaaata tgaggaacta tttttgttta gctattctta ttacttgatg caggacagct
    29341 ttttcacaac ctcatttcat gtgtttcaca cgtgaggaaa tttctttatg gcggccatgt
    29401 accatcggac gagcttgcgg atttcctaaa tagccacaag tacgtttgac tacatcacat
    29461 gtttttggat catgatttcc acattctgga cattcgaacc ctcgttctgt cggtttgaaa
    29521 tcaccttcga aaccacattg atagcaatga tcgattggcg tattcgtacc aagatacccg
    29581 attttgtcat atgcaaaatc ccatacagct tctaaagctt tcggattttg ttggagcata
    29641 gggtattcac agtagtggat gaatccacct gaacaatatt ttggataatc cttctcaaat
    29701 tccaattttt caaatggcgt tgggttttta cgcacatcat agtggaaact gtttgtataa
    29761 tattctttat ctgtgatatt ttcgatgatg ccgaattttt ctgtatccaa acggcagaaa
    29821 cggtcagtta gactttcact tggagttgaa tagacactga aatgataacc atattcatcc
    29881 ccccatgcat ctgcattagc ttttaattct ttgacgatat caagtgtgaa agcttttgct
    29941 tctgggtttg tttcccattc gccaccgaag aaagctgaag caacttcata caatccaatg
    30001 tagcctaacg atacagtagc tcgacggttt ttgaataatt cattgacaga atcggtacgt
    30061 gaaagacgtt tcccgaaagc accatacata tataaaattg gcgcatttgc tggaatggct
    30121 tctttacaac gttctacacg atagagaagc gcatctttca tgatttctaa gcggtcagac
    30181 agtaactgcc agaatttatt ctgatctcct tttgattcga tcgcgattct tggcaaattc
    30241 aatgtaacaa cacctaaatt cattcgtccg acatttactt cttgaccatt ttcgtctttc
    30301 cacccttgca agaatgaacg acagcccata ggtactttga aacttccagt taattcaacg
    30361 attttatcgt aattcagaat atcaggatac attcttttcg ttgcacattc caatgctagt
    30421 tgtttgatgt cgtaattagg atcttcaggg tttaagttca cacctttttt caaagtgaag
    30481 atcagtttag ggaagatagc tgtccgcttt tcactcccta atccattgat tcgaatctgt
    30541 aaaattgctt tttgaatttc tctttcaaac cagttttctc ctagtccaaa tcctaatgaa
    30601 gtgaaaggag tttgtccatt tgaagtaaat aaggtattga tttcatattc caaactttgc
    30661 atagcatcga agatatcttt tttggttttt gcttttgcat attctttttg gcgttctggc
    30721 gaatcgatcc attcttccgc gtcttttaga tgttttttat agttcaattc tgcaaaagga
    30781 gcaagcaatt catctgttcg gtcggcagaa cagccaccat attgacttga cgctacattg
    30841 gcaataatct gtgagatttg tgctgtggcg gtctggatag attttggcga ttcgacttcg
    30901 gcattgccga ttttgaaccc attgttcagc atgcctttga aatcaatcaa gcagcaattc
    30961 gtcatcggtg tataaggatg ataatccaaa tcatggtaat ggatatcgcc tttttgatgc
    31021 gcatttgcta tatgtggtgg caacattttc agtccgattg attttccaac gatcccggct
    31081 gtcagatctc gctgtgtatt gaaaacatta ctgtctttat ttgcgttttc gttcacgaca
    31141 gtttggtcct tgttgattag cttttcaatc gaaaagttga tatctgttgc tttactgcga
    31201 gcaaaatctc gtttggtacg ataattgatg tactcttctg ctaattcata ttcgtttttt
    31261 tctagtaata tatgttccac gatgttttga atctcataga ttttgacatt ttcagcaaac
    31321 cggcttttga tttcttggac aaccaagtct gcaatcattt gaattttttc ataagtaagt
    31381 ggtgataatg agccgtgaat tttcttttct gctttgatca gtgcttcatt gatcttttgt
    31441 tcatcgaaat caacatggcg gccatcgcgt ttgatgactt tcatctggga tgtctctggc
    31501 tgctgagtaa cgacagtttt ttcttcataa tttactttca ttatcttcaa ctcctgctaa
    31561 attgttcttg tctatcataa aacaaaatcg gggaaggagc aactaaatat cggctatata
    31621 tagtattcaa tgtgctgtat aattggattt ctatactata tgtagtgtat attttattgt
    31681 gaaaaaaggt gaaaacttgt agctggtgga tttgtttttt gcatatttaa ttttgctgaa
    31741 taaaataatt gaataaaatt ttttattcag tccaagtaaa agtgacaaaa acataagagc
    31801 aaattattcg tagatatgct actatgcaaa taaatagaaa tgaaaaatga aagaggagga
    31861 gaagaattgg aaaagatgat cgaagtgaaa catctaagta aaagctacgg cagcaaactt
    31921 tctcccgtca aagccttaga taatatctca ttcacagtag aagaaggcga gtttgttggg
    31981 attatgggcc caagtggtgc aggaaagaca acgctgatga atatcttgtc cacgatcaat
    32041 ttgcctacga tgggcagtgt ctcgatccaa ggaaaagaaa tcacaaaaat gaaaaacagt
    32101 gagctcagtg actttcgtcg aaaaaaacta ggattcattt ttcaagagtt caatttgatc
    32161 gataccctaa atgcaaaaga caatatccta ttgcccctag cagttgaacg gatgactaaa
    32221 gaggaaatgg aaaagagagt aagacatgta gctcaattgt tgaatataga agaactgctc
    32281 aaacggtatc ccgatgaact ttctgtcgga cagagacaac gaattgcagc tgctagagca
    32341 cttgttgttc agccacaagt gatttttgca gatgaaccaa ctggctcttt ggactcgaag
    32401 tcggctacag aattgttgaa ctacttgaag accatgaatc aaaaagaaaa agcaacgata
    32461 ctgttagtca cccatgatcc atatacagca agctactgcg atagaatttt atttattaaa
    32521 gatggtgtga ttttttctga agttgtcaga agagggacaa gaagagagtt ctttgagaaa
    32581 gtaatagaca tgcaggcgac gattggcgga ggaggaaaaa tgaatgctgt ataaactagc
    32641 cgttaaaagt ttttttcggc agtcacgagg ctatcttgtt tattttttca gtttgacttt
    32701 atccgtaatg atctattatt ccttcagcgc aatgacctac gatcaatctt tagtcagaag
    32761 agcaagtcaa gatacttcga tagatggtgg attgagtttt ggtggattta tcattactgt
    32821 tgttttgctt tttttcgtat tcagtgccaa ccgatttttc ttgaatagaa ggcaaaagga
    32881 aataggcatc tatcagcttt ttggtatgaa taaactgcag atatcaggta tatatgtgtt
    32941 agaaatcatg ataattggat tgttcgcctg tatttctggg atattactcg gtattatttt
    33001 ttccaaactc ttttcaatga ttttggttcg aatgatggat atggatctta ctagtccatt
    33061 ttttatctcc attccatcag tagtagatac tttatttgct tttttcatca tccttttagc
    33121 agtatctgta tattcaatat ggaaaatatg gcgctatcca atgattcgaa gttttggaga
    33181 aaaagaacaa gcagatagtt caacaatgcg cttcaggaca aggcaccgat tactcgcagt
    33241 catcgggtta ctacttttaa cgagcggata ttttggagct agtcatttta gggaaatcac
    33301 ttcatggatg atcagttatc attatcttga tacattagtt ttcttttttc cttttttaat
    33361 ttttttcgtt tgtgtggtcg gtacttattt gtttttctgt tatacatttc ggttattttt
    33421 gatcatattg agtaaatgga agatttatta ctatggtatc aattttttga taatgggtaa
    33481 cacgcaagtt catttgctca aaagttggcg tacaaattct ctgattacag tcattctcgg
    33541 aatttcttta atgacaattg gcggcacggc tagtatctct actattttat cctaccagga
    33601 gaaagtttcc tccccgatgg actatcagtt tgatgttgag acagagaaaa aaatcaagcc
    33661 gattctagca gaggaagggc agaagataac taaagaaatc accttgcatt ataaagtcat
    33721 tgggagtatc tatagtcaaa atctcgaaga acctatgttt caaactgcta atctgatcac
    33781 ggaaacacaa tatcaagcct tcagaaaagt taatccagat ttgccgaaca tttctttgaa
    33841 atcacctggt catactgttt tgcttgatca aatgcaaact ctttttcgga atatttcatt
    33901 atatggatct agtattggac tgccagataa tcaagaattg aagattcaac aaatgcttcc
    33961 aagtgtatta ggtgacgaaa caatggtata tctaagtcct gtactgattg tagatgatac
    34021 cgtttttgaa aaagcaaaag gtgtggatta tcaagtagtc aatgtagatg tatcaggtgg
    34081 aaataatgaa aaaatagatg aacgactgag caatgacctg tcgataaagt ggctcaatgc
    34141 tgtttattat tcttatgata tcgtaaatgg aagtctacaa ggagaaatta gcacaaagaa
    34201 acttccggat gaccaaatga aggaagcaca atttagccaa gaaagctctc gtttgaattt
    34261 ctcttccagc tattcgaaat tgcgtgcagt taatcggcga atcggtatca atatttttgt
    34321 cacactattt gtcgggatga tcgtgattat tgctactggt agtattttga cagtccggca
    34381 attagcagaa gcagaggaag aaaaagaaaa ttatcaccta ttgactaagc tggggattcc
    34441 acaaagaagg atcagacgga tgatcttcga gcaaaacgca cttaccttct ttccacccat
    34501 gatattaggg atcactcacg cagtatttgc aatcaatgtc ttcactcagt atataaaaac
    34561 tgccgattac tggttggctt atcttgtgag tggcttattg atccttatct atcttttgct
    34621 ttatgtcatt acgagtcagt tatattgtcg gattatcgag gaatgatata cggaataaga
    34681 caagataata aatagaacta aattttgaat accatttatt tggaataaga ggttgtgaca
    34741 cgatttttgt cacaacctct ttatttttta tattttttaa aagtacgaga aattataaag
    34801 aaaaaagcag tgatttgatg tgctattttt ataaaagcta tgtgacaaaa ctgttagtaa
    34861 aaaaacggtt tctgtaagct agacacatgt ttctttttgt caaaattagt tacactttgt
    34921 gtgaggttat atgaggaggg aatccaatga acaaatggcg ttctaatcca aaattgaatt
    34981 attggggaaa attcatcatt cgaacaattt tttatagtgc agtactgctg ttcctgattt
    35041 atctgtatca ctataaaaat attcaaggag gaacttttat ctataacgaa ttttaggagg
    35101 aagagtatgg aaatcaaaac gattattgaa gcaatcgatg attggggaat aaatgaacct
    35161 gatcgagttg cttatcaggc ggatgagacg catacttttg gtgaattgaa acaagcatca
    35221 gacgcacttg cttattattt agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt
    35281 ggcaatttgg agtttgaaat gattgtctca tttctagggg ttgtcaaatc tggccatgcc
    35341 tatttgccga ttgaagagca tacacccaag gagcgtatcc tatcgatctt tcgggtggct
    35401 aaaccatcta tggtcatcag tatcggggac tggatagaag atattccttc ggttcctgtc
    35461 ataacaaaaa aagaatttca agaaatcaag aagataccag taggatttta tcccagaaat
    35521 agcgtaaaag gtacagaaaa tttttatatt atttttactt ctggtactac tggagaacca
    35581 aaaggcgtac aaatcagtca tgataatcta gtcagctttg ttcaatggac gatggaagac
    35641 tttgggatca agcctggtat gcatttctta gcccaagcgc cattttcttt tgatttatcg
    35701 gtattttcta tttatcctgc attagtttct ggcggaatgc tgaagccgtt aaataaagca
    35761 gttgttcaag actttcgtca attattcgcg acattgccag ccttgaagtt gaatgtgtgg
    35821 gtttctactc catcatttat ggatatttgc ttgatggaac ctacattcaa tggagaaaat
    35881 gtgccggaat tggacatgtt tttattttgc ggagaagaat tgacgaaaaa aactgcagaa
    35941 agtctacttg atcgattccc aaatgcacgg atatataata cttacggtcc aacagaagca
    36001 actgtagcta tttcaagtat tcagattgac cagcatgtac tagatagtta tgaccgacta
    36061 ccgattggct acgtaaaaaa agacacgaaa gtttctattg tccaagaagg taaacctgtt
    36121 tcaaaaggag aaacgggtga aatcattatt gcaggaccaa gcgtatcaaa aggttacctg
    36181 aacaatcccg aaaagacagc tgctgcgttc ttttgtcaaa atgacttagc aagttaccat
    36241 acaggggatg caggtagact agatgaatat gggcttttat tttatgaagg acgtatggat
    36301 cagcaggtca aattacatgg ataccggatt gaactcgggg atatcgaaca ttatctgctc
    36361 caagacaacc gaatcaaaca agctgtcgtc gtaccaaaat accaaggaac aaaagtacag
    36421 cagctggttg cttttgtcgt attagaaaac aaaacgaaac agccggattt tcagttgact
    36481 aaatctatca aagaacaatt actagaagtt gtaatggatt atatgattcc tcagaagttc
    36541 cattatgtcg atctgctgcc tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca
    36601 gctgaggtga atcctgaatg atgttacctc atatgatccc ttatgcagag ccgttttatt
    36661 tttttctttt agccattgcg cttgtaccga tcgttctatc tttattgata tgggaaaaaa
    36721 gattaccagt ttatcaatca gcagtgacac ttttcttttt atttgtcagc tttggaggta
    36781 attattggca acagggagcg gcgctgattg ggtatgtttt gtggcagacg atacttgtat
    36841 ggggctattt tgtttatcgg caaaggaaaa atcagacagg atggtttttt gccgccgtta
    36901 ttttagcgat aattccactg tttttaacga aagtgactcc tttttttgaa catggaaaag
    36961 catcgttact tggtttttta ggaatctctt atttaacctt taaatctgta caaatgatca
    37021 tggaaataag agatggcatg atcaaaacat ataagccatt tcgttatatc cagtttctgc
    37081 tgttctttcc aacaatctct tctggaccaa tcgatcgcta taggagattt gaaaaagatc
    37141 agctccatcc gcctgttcgt gataaatatg ttgatctttt gggaaaaggc gtccaaaata
    37201 tttttgtcgg ttttttatat aaattcatta tcggttatta ttttggtcaa gtactgcttc
    37261 ctgtagtggg acgcattgcc ttacagcatg gtggtatttc atgggcatta gtaggttata
    37321 tgtacgtgta cagtatgtat ttgttttttg attttgctgg ctatagctta tttgcagtcg
    37381 gtacaagcta catgatggga tatcagacac caatcaattt caataaacct tttttatcat
    37441 ggaatatcaa agaattctgg aatcgttggc atatgacgct gtccttttgg ttccgtgatt
    37501 atgtatatat gcgattgatg ttcttcatga tgaagaaaaa attttttaaa agcaagatcg
    37561 taatgtcaaa tatcggttat tttgctttgt ttcttctaat gggtatatgg catggattga
    37621 cgtggtacta catcgtatat ggactgtatc atgcttgtct gatctgtctg acagatgcat
    37681 ggatccgatt taagaagaag cataaagagc agttgccttc taacaaattt acgcatggct
    37741 tagccgtatt tttgacattt caagcagtat gttttagctt cctgattttt tcaggatttt
    37801 tagataaact tttttttagc taacgaatag tgacatatta atttatataa caaaggagaa
    37861 aaggaaaatg gaagaacaaa ttttatcaat cttagaagaa ataacaggaa cagatgaagt
    37921 aagaaaaaat cgtgatattg atctatttga tgaagggttg cttgattctt tagcatctgt
    37981 tcaattatta gtcgagctag acggagagct ggatatccaa gtccctgtat ctgaattcga
    38041 gcgagaggat tggagcacac cgaataaaat cattcaacaa gcaactgcat tgaagggttg
    38101 agtatgaaaa agaaactttt tgctattttc ggcccggttg ttttagctgt tggcttactg
    38161 tttgtgtttt ttacctcctc ctatcaagta gatatctcta atccagaaac gattagacgt
    38221 gcctcttctt ccatgtcagt gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta
    38281 tctgaaaaac aatatattcc tttttttggc tcaagtgaat taagcagaat cagtccattt
    38341 catccgtctg ttctggcaga aaaataccag cgaaactatc gtccattttt gctgggagca
    38401 cccgggacac aatcgttaag ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa
    38461 aacaagaaag tcgtctttat tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact
    38521 gattacttta atacgtatta ttctgaactt caaacatacg actggttgtt ttcaatgaag
    38581 aaagtaacgc ctgctgatcg ctatttagct agaagattat taactttctc aaaagtaaaa
    38641 gagaatgata cgttgacggc gattctgcaa acgatcaaaa aagggaagct acctttacct
    38701 gaatctttga atcagctgcg cagccaatgg aacatgctga agagagagga tgaagtattc
    38761 agcaatattg ggttgaaaga tcgacagcaa aaaatcgatc atgagtcaaa acgattacca
    38821 aaacaatacc aagaaacaga attatccatt ttagcaaatc aaattggaga acgagaaacg
    38881 acgaataatc cattcgggtt gaaaaacgat ttttatacgc atcgtatccg agcccacgag
    38941 ccagagctca aacagtcaca aaaaaattgg gattatcgtt tttcgccaga attttctgat
    39001 tttcagctgg tcttggatca attagcaaaa aaccataacg aagtattgtt catcatccca
    39061 ccggttaacg agaaatggag cgattatacc ggactatctc aagaaatgtt acaaggattt
    39121 gccaaaaaga tcaaatttca attaaatagc caaggattca atcgaatagc tgattttgtc
    39181 aatcaagcgg gtaccaacta ttttatggaa gacacgatcc atctaggctg gaaaggctgg
    39241 ttggctgcag atcaacagat cagaccgttt ttagaagaaa atcatattac tgctagtaag
    39301 tatcatcttg acgatgcttt ttttagcaaa tcttggcaac atcaaatacc agataaacta
    39361 caattaaaat aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt
    39421 ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa atcataagca atggtataat
    39481 cacaatatga atatagaaat gggggaaaac atttggatac aaaagaagaa cagctactta
    39541 ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga gaagtattca
    39601 gagaatatgc caaaccattc gctgatgata ttttttatga cggtttaggc agtgtgattg
    39661 ccaaacatgg tgctggcgga ggccccaagg tctttatttc tggacatatg gacgaagttg
    39721 gttttatggt aacaaaaata accgaaaaag gattcttgga attccaaact cttggaggtt
    39781 ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg
    39841 tccacggtgt gattggttcc aaaccaccac atgttttaac acctcaagta agaaataaac
    39901 cttatgaaat aaaggatatg ttcattgata ttggcgcatc aagccaagaa gaagccaaag
    39961 agtggggcat acgtcctgga gatatggtca ctccatatat tgaatacaaa cgaatgaatg
    40021 gcagtaaata tttactggca aaagcatggg ataatcgtat cgggacagct gtttctctgc
    40081 gagtattaga gaatctatct aaagaagcac acccaaatgt tttattcgca ggaagcgatg
    40141 tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac acaccttgta aatcccgata
    40201 ttgcttttgc tttagatact ggtacagcag gagacactcc aggaatgaca ccaaaagaag
    40261 cagattcgat tttaggaaaa ggcccacaga ttttgatttt tgatgcatct atgatcccgc
    40321 ataaaaaatt attgaatttt gttattgatg tagcagagga aatggatatt ccattccaat
    40381 atacagtgat ttctggcgga ggaactgatg caggacaaat gcatttgaca agagatggtg
    40441 ttccgtctgt agccattacc gttcctgttc gttatctgca ttcccataca tcagtgattc
    40501 atgaagatga ttatttgaat acagtgaaac tagtcacaga attagtgaaa cggcttgata
    40561 gtgaaacagt tgaaaaaatt cggagttatg aataatgaat aatagaatac ctatttctga
    40621 atatcggaaa aaagcacgag tttctttgga aaatcaatgg ggaatcaata cttggcttgt
    40681 tttcttatct ataatattta cgagcgtcat ccagaacttt tttgacggtt tcagtccttt
    40741 tccggaagaa agcatgcagc agcacttatt gagttttcta gtcgataatt tacttttatt
    40801 cgcattcaac tatgcattgt tttacattgc gctagtagtt gtacggggtg gcagaccaaa
    40861 aagtaacctt ttattttcag ttttccaaag aaagtatttt ggttcgatac ttggcatcaa
    40921 tttgatcaat tcagtcatca actggctact caacagttta atctttctgc ccatgttttt
    40981 aatcagcgga gtgagtactt atatgcagct gcttttcagt tcaaatgcgg tacctacggg
    41041 tgtcatgcca gaagattttc ttgatatcag ctttgtttta tcgatgatag ggatgatttt
    41101 tgtttcagca ctcatcatga gtattgttgg cggtttattc caatttgctg catggacaaa
    41161 gttcgactat cctgatttgt cggttattca atgtttgaaa tatggctggt ttttattgaa
    41221 agatagaatt ggtacgtata ttttgttgca gctgtctttt atcggctggt atttgctagg
    41281 agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc
    41341 agaattttat gaacaagcac ggattgaaaa aggaggtcca gacgcctatt ttacttagca
    41401 tgtaagcagt ttattcatca aaaaaataac ttttgtggta ggataaatct ttgaggggga
    41461 atgtacgatg aaattaactg ttttaggatg cttaggagcg tatccttata aaaatcaagg
    41521 gacaacaggt tatttgcttc aatctgacgg gttcaatttg ttgatcgatg caggtagtgc
    41581 tacgctgatc aaattacaag aacaccttga tccacttgat ctagatgctg tgatccttag
    41641 ccattatcat catgatcata ttgctgacct tggtgtatta cagtattact ggcaacttca
    41701 tcctaagaga aatccggaga cattgctttc aatttatgga cacacaaaag attccattca
    41761 ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat ccttattttg aagctgaaga
    41821 attaaaaatt ggtccttttc tgattacttt tatggaaaca atccatccag tagtatgtta
    41881 cgccatgcgt atcgtagaag aaacaacggg gaaagtattc gtatttacag gtgattcagg
    41941 ttatttggaa agtttcgctg attttgcgaa agatgctgat ttgtttctag cagataccta
    42001 tctttttgaa ggaaatgaac atcatcatgc ccatttcact tcaaaagaag ctggagaact
    42061 tgcgaaatca gctaacgtaa agaaacttgt acttacacac ctacctcagt ttggtgattt
    42121 agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata tagaacttgc
    42181 tgcagttgac aaagtgttta acatttagga ggaagaacat gatttttgta ccaaatgaaa
    42241 accacgaccc aagaatcaat ttagcaattg aacaatattt gctgcaagaa atgcctttag
    42301 atgaacctat tttgctcttt tatatcaatg aaccttcaat catcatcggc agaaaccaaa
    42361 atacgcttga agaaatcaat cgagattatg tggaagaaaa tggcattcat gttgtccgtc
    42421 gcttaagtgg aggcggagct gtctaccatg attttggaaa cctgaacttt agttttatca
    42481 tgcctgatga tggggattca ttccgtgact ttgcgaaagt aacacagcca atcatccaag
    42541 cacttcatga attgggtgtt gctggggcgg aattgaaagg ccgaaatgat ctagtcattg
    42601 atggcatgaa attttctggc aacgcgatgt atgctaccaa cggtcggatg tttgctcatg
    42661 gaacaattat gttcgactct gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag
    42721 acaaaatcga atcaaaagga atcaaatcga tccgttctcg tgtaacgaat atcaaaccat
    42781 ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga
    42841 aaattttcgg cgtagagtca gtagaagaag taaaaacgta cgaattgact gatgaagatt
    42901 ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa ttgggattgg aattacggga
    42961 agtctccaga tttcaatttc tctcgccaaa aacgtttttc tatcggttcg attgaagtcc
    43021 atttgaatgt ctcagaagga atgatcaaag aagcaaaaat ctttggcgat ttcttcggac
    43081 taggagatat ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat aaagcatctc
    43141 tatcaaaaat agtcagccaa attgatgtga agaaatattt tggtgcgata gaaccagaag
    43201 aattgcttac cttactttat tgaaaataaa aaatagcgga atccacatat atggattccg
    43261 ctatttttta tacaggattc gggtaatttc gagagagttg aacagtgatc cgatcaatga
    43321 aagtatcttt ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct ttcatcatca
    43381 ttaagtcaaa acgttcctga tcatctaatt cttctgtgat gcctcgacta aataggtttg
    43441 tatcgtaatg tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg
    43501 acaaataaat ttttcgatac agtggatttt cgatggcatg agtttctcta aaggaatgct
    43561 ctaaagcgtg gatcaaaagt tgagccagat cgtcatcgtt gataggaagt tcttcgggaa
    43621 gatctgcttg gaaggttatc tgaatattcc gtttggcagc taacttctgg taacgcgcta
    43681 agataagatt tgtcacctgg tggttgcaaa aagaacacag catttcgaaa ttttttgctt
    43741 cttctaatat cttttccaca tagtttttag cttttgtttg gtccgtatgc attaaatctt
    43801 gcagattctc catctcttgg acaaattcca ggcgaagtgt atttacttct tggatatgct
    43861 gtcgaagctg ctcataatgt tgctccaaca aatcattttt gatttttaag aaatctactt
    43921 gtctccgatc tttgtcggta tttaagatgt gatcgattag attgtagctg ataataacga
    43981 ttagaatgat aatatataaa atcgtagacc aattgatcgt tgtttgatag atacctaatg
    44041 ccgtttggat gtaaatcaag caatggataa tggctaccaa gacgatccaa ggtgtacaag
    44101 ctacataaaa acggttattc ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag
    44161 tgcttataac actagcaata ttagcaatcg gcattaattc aggaagttct gcaggagtaa
    44221 aagttgcata aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt
    44281 accatttttt atagtaaaac attcggctcc aatagtagca gatgatgaaa gctgacgtta
    44341 agataataaa taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta
    44401 aaatatcttc tgatacagct tcaaatccta atgccaaagc aaagcaactt aagagaataa
    44461 gtgagtattc aagacgtttt tgtttataca tcaagtagat cgtatagaaa aaggtcccta
    44521 tagcgataaa aacagcgatt atcatcgtca atacttgcgg cactgattgt gaataaataa
    44581 aactaatcaa tggtccagtt gttcctagat aaactttcgg tggcagtcca gcatagttct
    44641 gataaggaga tgagactgta agggtcaatt tttttccgat ataatttgct ggtaaatcta
    44701 tcacagccaa tgagagtccg ggattgcttt cttcctgata atctttccgt tggtataaaa
    44761 tcttttcgcc tagatataca gtaatccatt gatggtttcc tttgattagt aaatttgctt
    44821 cgtccaacgg tttttcaagt gtcgtttcca taatgaatgt ttcgtcttta tccacggtag
    44881 gaatcgtacg agtgtacttg ctgtcaaact taaaatcagg atcatctttt gtatagaaaa
    44941 cccacgaatc ttttatttct ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa
    45001 agaaataagc aattcctgtt aaaaataaca atgataatat aatgattatg ctccacttta
    45061 tacgtaaata aaaagattct tttttattat atatgttatt aaaaaaagac ataaaggtat
    45121 tcctttctaa aacttgtaat gttagggtaa acgtttttta aactaacgtc aattatttat
    45181 aataaaaaat gagataaaga tgaggaaata gaattagaaa catataatta ttaaagggaa
    45241 acatttttac agctataatt tgaaaaaaat ttatcgttat tatatgatta agaaggattg
    45301 aataaagaaa gaagttgaaa acgatacctg attacaaaaa aataactttt ataagttcta
    45361 taacgttgat tatattttta attcttattc ttctcgtcgt cttggttccc cgaatttttg
    45421 gtttgactcc ttatattatt gaggacaaag aaatgagtcc atattatgca caaggaaatc
    45481 tgatatatgt acgaccgaaa aatacaaatg agattttagt cggagatatt attacatatt
    45541 atgaaaattc aggaaaaaaa atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg
    45601 aagattttta tacaaaagct gatacaaaga cttatattga gcctggagta gtaaaaaaga
    45661 gaaatattat cggtagtccc atctttcaga ttccatatat tggactagtc ctttcaaaaa
    45721 cggcatttgc atggattcag gggttattct ttacgattgc tttttgccta acaggaatca
    45781 cagcatggaa tacctttgta gagttgaaaa agaagcggag acgaacacag aaatttttgt
    45841 aagaatgtta aaaacataaa tgtattccag agatccaacg actcttctga acatgaaaca
    45901 cgagaaaaca ctcatgcagc ttgtagattt agtcagttgt tcgtataatg tgtgaagagg
    45961 aggagtagta tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga actattggat
    46021 tttatgcgta cccagcaaaa aaaattaccg ggtgagctag gcaaactgga agaagaagct
    46081 catgtagcag aagtaccagt tattccccat gaaacagttg tatttttgaa atttttactt
    46141 ggacagcttc agcctgaacg aatcttggag atcggagcag ctatcggttt ttcatcaagt
    46201 gtcatggcca cgacaatcgg agaaaacgct catgttacga caattgatcg tttcgatgtc
    46261 atgatcgaga aagctaaaaa aaactatgaa cgtctaggac tgacagataa agtgactttg
    46321 cttgaaggtc aagcagcaga tatcttgccg gagttgtctg gtccgtatga ctttattttt
    46381 atggatagtg caaaatcaaa atatattgaa tttttacctg aatgcttacg tttgttaaga
    46441 aaaggtggcg ttttgatggt agatgacatc ttccaaggag gaacgatctt gctcccagat
    46501 gaagagattc cccgtggcaa acgagctatc catcgtaaat taaatgaatt tcttcgtgtt
    46561 gtgatggatc atcccgattt gacatcgaca atcttgccat taggtgatgg tgtgatctta
    46621 atgacaaagg aatcagaaac aatcgagttg tgatacatga ttatggcagc ctgtagataa
    46681 tcgattgaat cgagggctca attcgaactt gatgtaaaag tttctatatt tgtaagtcaa
    46741 ttaaaggata caggaaatct aaataaacag gagtagcatg aagtcaatgt agctcctgtt
    46801 ttcaattgat aaccttagaa cagctgaagg agaatgtctc gttttaaaag attgatttag
    46861 tagttgggcc aaaataattc attatttgct aattaaataa ctatttcgta ttgacagaag
    46921 caatttaatt atctataatt attgatgttc cgctgattct tgtcatagta gctcagctgg
    46981 atagagcatc cgccttctaa gcggacggtc gggggttcga atccctcctg tgacgctaaa
    47041 aaaccgttag aaactgaggt ttctaacggt ttttctttgt tttttgtgcc aatattgttt
    47101 gttttcaaag gcaaataaaa taaaaaaaca tccttatcga gtacaattat aaggagcaaa
    47161 aaaataaaac tcatcagaaa agaggaagaa gatatgacga tactatctag aattttagtg
    47221 actctagttg caattgaatt tttttatatc atgtatatcg aaacggtgag aactgattct
    47281 gacacgacaa gtagggtttt taaaatgagt aaagaagagt taagcagaaa atctgttcaa
    47341 acattgttta agaaccaagg agtgtacaat ggactattag gagtgggact actttatgga
    47401 gcttatcttt cttccgcatc taaagaaatc acaagtatgc tattaatatc tatttttttt
    47461 gttgcattat atggcagttt gactagcgat cgttggattt tactcaaaca aggtgggcta
    47521 gctctgttgg cattgctttc tattttctta taaatataga aaaaataaga atttatctaa
    47581 caggaaactt tgtgaaaaat attactttat ctcatgaaat atgatattct aatgaatggt
    47641 attttagaaa atttcatgaa aaaggagtta gataggtgtt caaaaattat gtttcctcac
    47701 taaaaaaaga attttcaggt tatgatcttt caagatttca gaaagatctt ttggctggga
    47761 tcacagtagc tgctgtagca ttgcctttag ccttggcttt tggggtatca agcggagctg
    47821 atgcggccgc aggtttgatt acggctatca ttgccggtat cgttattggc ggtttatcag
    47881 gcggttttta tcaaatatca ggtccgacag gtgcgatggc agctatcttg atgtctattg
    47941 cggcaacaaa gggaatgcaa ggtgtattgc ttgctacttt tttagcgggt ctatttttac
    48001 ttgcagcagg acttttccga ttaggaacac tgacttcttt tatcccagca cccgtaatta
    48061 cggggtttac ttcaggtatt gctatcatta ttgcattagg tcagatcgat aatttatttg
    48121 gtgttcactc ggaaggtaca aacgttattg aaaaactaag tagttatcaa acgataggat
    48181 ttccgataaa cacagcctct ttgatcatgg gagggctagt cattttaggg atgtttatct
    48241 atccaaaaaa atgggcgaaa cgaatgccta gttcgctgct ggctatcatt ttggcaacag
    48301 cagttatgct attgttccat ttaccagtag caactgtggg taagattcca caaacattga
    48361 tcagtagtaa tcgtttgaat atgggagatt ttagcttttc tgccttgcaa caagtggcag
    48421 ttccagctat tagcattgct ttattaggaa tgattgaaag cttgctatgt ggtgcttcgg
    48481 ctggccgaat ggcaaataag cccttagata gcaatcaaga attaatggcg caaggaattg
    48541 gaaatctgtt gcttcctttc tttggcggga ttcctgctac tgcggccatc gcccgtacaa
    48601 gcgtggccat caaatctggt gcacaaacta gattagctgg tatgatacat gcagttgtct
    48661 tactgctctc catgcttgtt tttgcgccag tgatgtcaca gattccaatg ccagcacttg
    48721 caggtgtttt gattgtgacg gcttggcgta tgaatgaatg ggaaacgatc aaggagctat
    48781 ttactaataa atattggtca gcagttctat tgtttatcct tacgatgatt tgtacagtta
    48841 tctttgattt aagtattgct atcgtgatcg gggtaatcgg cggctgtgta ttttttgttg
    48901 tcaaaagcgc tgctatcacg atttcattag aaacgatcga ttggcaaaga atggggctgc
    48961 cagaaacgga aaagctgaat aactggacag tagtgtatat tagtggtcca ctgtttttta
    49021 tgtcttcaga aaaactcaaa aataaattgg aagaactaaa tcatcacgat ggtgtcattt
    49081 tctccatgcg aggcgtacca agtattgatt tgacggcacg tgcagttttt gatgaatttc
    49141 aagaaaaagc agagcaaaga ggacaaacga ttatctatac ttccttgcag ccagaagtag
    49201 aaaaacaact ttctcacttg tgggaacata cattcacgca tcaacatcaa acagttgctc
    49261 atgcgttgac agctttacat gcgaaatata ctgcaattag ccattaaaac aatgaaaaag
    49321 aacagacaag actgtaaact gaagatttta tggtcttgtc tgttcttttt aatattgctg
    49381 taaaagtgaa gctagtactt gccagtcttg atggataaca ggtacaaggg agcgattgta
    49441 gaaaatagct gctggatgaa aaagtggaag aattcgatag tttttttctg accattcgta
    49501 ggcatccttt tgttctgtta attgtaagac aggccctgta tagagttgac catgattttt
    49561 ggtaaccgta tattctttgc caagtaaacg ttgaagacca atgtttccaa gtgtcgcaat
    49621 taattttggc tggaccttca tcagttcgta atccaatatt ggtgcatgcg caaatacttc
    49681 ggaacgagta ggtgtccgat ttggataaac agtttctgac tgttgtgtcc gtttattgat
    49741 ccgatgagtc acgcgatacg ggcggctcct tacagcactg gtgatataca cctcttctcg
    49801 tgttaagcca gttgaagaaa gtgcctgcat caattctttt ccagcttgac cgctaaaagg
    49861 aacatgcgta tcaatctctg ttttccctgg ggcttctccc acgagcatca gtttaggatg
    49921 aagaggtcct tgcccagcga taaatccttc tacaggacgg ccgttcattc ttaaagaagc
    49981 ttcttgtatc agttcttttg ggtattccat ttatgctcct cctttttctt ttattataag
    50041 aataaaagaa acacaatgcg actattttat ttggtagaac tgtgaggata gaaaagcaga
    50101 agtattgctt gaatttttgt tttacttttt gattttaaat ttagttgact aacggttgct
    50161 gttctggtaa gctagtactg ttgtaattgt ggactccaca gctacaaccg ctcagaacaa
    50221 gtaataagca cagcatatgc tgtcacttcg gatggcgagt ctaagtatat cagaaggagg
    50281 tgctttatag catgtacgca attattaaaa ctggtggtaa acaagtaaaa gttgaagtgg
    50341 gacaagcaat ctatgttgaa aaacttgacg ttgaagcagg agaaaaagtt gtttttgacg
    50401 aagttatctt agtaggcgga gaatctacaa aagtaggcgc tccaactgtt gcaggtgcaa
    50461 ctgttgaagg tactgttgaa aaacacggca aacaaaagaa agtcgttact ttcaaataca
    50521 aacctaaaaa acattcacac cgtaaacaag gtcatcgtca accatataca aaagtggtta
    50581 tcgacgcgat caacgcttaa tagcgtttga gaaagaaggc ctatatatga tcaaaggaac
    50641 atttaagcga aatgacgcag gtcagatcgt ttcattcact ttgaccgggc atgctgatgc
    50701 tggtccttat ggaagtgata tcgtttgtgc gggtgtttct gcgttagcga tcagcactgt
    50761 caacgggatt gcctcattag ccggttttga gccgattgtt gaaatgaatg aagaagaagg
    50821 cggttatctt tataccgaag tcacttcagg aatgacacaa gaacaaaaca atattgctga
    50881 gattctatta gaaaatcttt tattaggatt acaatcgata gaagctgaaa actcagaata
    50941 tatccaaatt aaaacaatta atgataaata ggaggtgcag accatgttgt tatctatgaa
    51001 tctacaatta tttgcccaca aaaaaggtgg cggttctaca tctaacggcc gtgactcaga
    51061 atcaaaacgt ctaggtgcta aacgtgctga tggacaaaca gttacaggtg gatcaattct
    51121 ttaccgtcaa cgcggtacaa aaatctatcc aggtgtaaac gtgggtatcg gtggagacga
    51181 cactttattt gcaaaagttg atggcgtagt acgtttcgaa cgtaaaggcc gtaacaaaaa
    51241 acaagtgtct gtttatccag tagctcaaga agcttaataa ataaactgct ctatctcttg
    51301 tgtagcaagg gatggagctt ttttgtaaat tatgggattt tttttgacca tacttttgac
    51361 catactttct tagaagttaa tataatttgt tagtttttgt gttgtttctt cttttttgct
    51421 gttagtaatg tgtgtatatg tgtccatagt agtttttata tttgcgtggc ccattctttc
    51481 ttggacttct ttgtgatctg cgcctgcctc atatagcatt gatgcatgtg tatgtctaaa
    51541 accatgcaga ccgatataag gtaagccggc acgctcacga tatatattgt atctttctgc
    51601 cactgactga ttaattgtga aattattttc gtcattggta aacaaataat tagctttagt
    51661 aaatcctaat tgaaataagt actttctttg ttcgagcttc caaaatttta gttgctttat
    51721 agttttttca tctaaataaa ttattcgatt tgattttttg gttttaggag tttcagatat
    51781 ataatattta tctgaacgag ctacagtttt tttaatggcc atttcatttg ttttaaaatt
    51841 aatattgtcc caagtgagtg ctaatatttc gccgattcta catccgctga atgctaatag
    51901 tctaaagagc gtatagtctc tgtttttaaa atatggattc tgttcttgag atacttcatt
    51961 tagaaataat tccaactgtt cttttgtata taattttaat ttcttttctg ttttaatttg
    52021 tggctttgga ataagtaaat ttaatgttgg attatcagat gttaaaccaa tatttatagc
    52081 atacttaaaa actttattca tatagtttat gaaaagaggg tactgctttg aagttccttt
    52141 ttcactccaa tcatttacta ccttttgaca atatgccgta ttaattttag aaagcttaat
    52201 atttccaaat ttgggatata tatgtttatt aaagattatt ttagtccttg aatatgaact
    52261 ttcttttaca gtttttttgt agctttctag ccataattct gctgcttctt tgaaagtagt
    52321 atttgtactt gtgggcattc ctgttttttg taattctagt tctaacctag aaagtgctgt
    52381 ttttgcttct ttttgtgttc taaatcctcg tttagtagta tattttttct ttccagttag
    52441 cggatcaata ccgagataag ttttaaagta ccatgctttt tcgccatttt tctttttata
    52501 ttgttttatc attgccatca atattacccc tattcaaatt ctaattctgc catgctatca
    52561 ttgaaaaatt cgatcagtcc ttgttcctct ataaattcaa gccatatttc cttcccgtta
    52621 attaatggaa ctcctttttc tttacgaatt gtttgataca agttagtcac gacttgtttc
    52681 cacatttcta atacagcttt atctattttc ttatcatccc agcgtttttt ttggggtcta
    52741 tttttgtttt tttcaatttg ataattagta gctttgatga taaatgtttc gtatccggtt
    52801 gtgctttttt ctaaccattc tttgaattct gtatatatca tttttgttca cctttctatt
    52861 tttgaacgta tgttcttttt tgcttaaaaa attttagaga gctgcataac aatagatgcg
    52921 agcttttaat aattctttca caagtggttc gaagcgaacg cttaaattgt tatcttctat
    52981 aaatttcatg taattgatat tgtgtggttc taattcagac tttgcaatat atctatctaa
    53041 taatttttca atcatgaagc gatcagcctc atattccatt tttgaatgga gagagaatgc
    53101 taagttatat aaaaaataat tatcatgatg tttagaagcg tgtcctaatt catgtaataa
    53161 ggttctttgt cttctatact tagataaatt agcttttatt actatggtat tcatacaagc
    53221 aacataatgg ccatcggcat caaggttttc acgctcttct accttaacgc cgagttcatt
    53281 aattatcatt tcaatttgac tgtccaaatg actcacctac ttatttagtt atcttttttc
    53341 tctaaatatg cttcaatcat agctgatagt atttccctat cattatcggt gagcggtttt
    53401 ccgtcgctac tcataacaga tgctaaagct tcttccacgg ttagttttct ttcctctggt
    53461 gcaactcccg cgttaggatc atctgtgcgt cctagcaaat aatctgttga tacatggaag
    53521 tagtcagcaa ctttttgcaa agtttctatt ttaggacttt gtgtcttcca tttataaata
    53581 gagttttttc caaatcctat tctattttct aattcagaaa tagaaatacc tcttttttta
    53641 cagagctctt taaccctttc gaaaagcatg tcaaatcaac cttcctagag cgtacgataa
    53701 taatttatac aaaaagatag aaaggtagtt gacaaatcta tccaattgtc ttatatttaa
    53761 ctcgtaagct aatttaataa gctaacaagc aacaagaaat acacctaata aaataaacga
    53821 attcgaggtc ggcaaactaa gaatgttaat ttttagggtt tttctaggct tatttaactg
    53881 tatataaata taagacagtt ggatagaaat gtcaataaaa attagctaat tttattagct
    53941 tacatattta aagaaaagga ggaaacatca tgaaacaaaa taaaaaaact ccttccgcaa
    54001 aaggagcttt aataacttta aattttaaag accaaaagtt tagtgctgtt gttgatgaaa
    54061 ttcatccaca agagcatcaa aaaaattctt ttgcaactga cgagaaaaag actcagaaac
    54121 gctctgagaa agttcttcga ataattcaat ttctgttgga aaagattcgt cattttggaa
    54181 gtctattttg aattctttat taacagtctt gaatacggtt tgtttgaata aggcgtcgac
    54241 atccatatta aattcttcca tactattttc acctcgcttt ctattcaaaa ttatatcaaa
    54301 gaaaggaatg agcaaaaatg aacacaccgc aaatttttaa ctttgaacaa catgaagtaa
    54361 gaacagtaac aatacatgat gaaccatttt ttgttggaaa agatgtggct aaagttctag
    54421 ggtaccaaaa tggtagtaga gatattaaca gacacgtcga tgtagaagat cggcaaaact
    54481 accaaaacgg tacttttgaa tcgcctagag ggttaactat tattaacgaa tcaggtttgt
    54541 actccttaat tttaggaagc aaacaaccaa atgctaagaa atttaaacgt tgggtgacta
    54601 gcgaagtgtt gccagcaatt agaaaacatg gaggttatct aactccagaa aaagtagaag
    54661 aagctttgct taatccagat acaatcatcc aattagcaac taagttgaaa gaagaacgta
    54721 ctggaagact aatcgcagaa caaaaaattg cagaatacga acccaaaatt tcgtatttgg
    54781 atagtatctt atcttctaca gattcagtaa ctattagtca aatcgcggct gattatggga
    54841 tgtctccaca acagatgaac aaactacttc ataagctagg tattcagaaa aaagtaggca
    54901 accaatggtt attatgcaaa aaacacatga gacagggtta cacaaaatct catacaactg
    54961 agattccgaa atctgatggc ggaacaaaag ttgtgatgaa tacaaaatgg actcaaaaag
    55021 ggcgtctgtt catttatgag tcgctgaaaa aagaaggata tattccagaa atcgatttat
    55081 tagaggagga atgacgatgt cacaagaatt tattttgaaa gtcagaattc aattagcaaa
    55141 atacggaaag tctcagaact ggttagctga tactatcgga atttctagac catatatgtc
    55201 ggacattatg aatgggcgta gaaaaccaga taaacaaatc aagccaattg aggcagcact
    55261 agcagagtta gagaaggaga aataacatgc aaataacaat tccagataat ttagtagttt
    55321 ccgaattaac tacacagatt acgaatgctg ttcttaattc attggacgaa cgattacacc
    55381 ttatgaacaa atcagtagag cttcctccat atccaaacaa atcagaggta aaaaaagttt
    55441 taggcattgg tgatgacaaa ttaacacatt ggataagcct aggcttaaaa acacagcagt
    55501 ggagcaagtt agacatcaga attgaacgat cggaactcca aagatttttg aaagaaaact
    55561 ttgagttcta aaggcaaagg agaatgattt tatgtcctac acattgcaac aagaacatca
    55621 aattctaggt ctgattaaac agcgtagaaa acaattacaa gatgatcgtg cagcgcttag
    55681 aaaatccgat gagctatcag atagacaagc tgaactaatt gcttctgaac ttgaggattt
    55741 gagaatgcta gaaataaaaa atagggagat tagattatga agaagacaga cacacttttt
    55801 ataggattca ttttggggtt attagtgatt gtagcgcacc aaagtattat cggaggaagc
    55861 ttgttcgcag cattgatggt tttaatcaat ctgcttgact caaaagaaag gagcaactat
    55921 ggcacgagaa gaagcgctaa aaatcggtaa agtgattgct gataattggt gggcaaatag
    55981 ccgttctatt attttaagca agcaacatat cgaaaagaaa aaagcatggc aacaaataaa
    56041 aaagtgactc cgccgaccaa agcaatgagt cactcaaaaa atcataacta aggagatttt
    56101 aacatatgga aaatgaactt tccactctag atcaatattt ggccgatcct gattggggca
    56161 aatcgaattt acaactggtt gctaacaagt acgaaaaatc aattgatgaa gcaattgttg
    56221 cattgactga tgcagcttcc ataaccgctg atgacagtaa atggaccaag agagacttgg
    56281 gcattctaaa acgaggcgtt aattggcttg aagatcagta cagagaagaa acaaaagaga
    56341 agtgatatga gtgtttaaac cactaataga ttcatactcg gcggtactaa agaaatttaa
    56401 aggtaacgac attggtgcaa caatcaacga agaagtaaat atcgaacggc tgaaaacgat
    56461 gtatgacgga tatgatggcg atcgagtcat tgaaattcgc tttattgatc cacgacggtt
    56521 cacagtacag caaagaaact tcatctatgc gctcataggc gatattttca tcgatacagg
    56581 catgccaacg gacttctgga aggaattctt ctacttccgt tttgaaggtg tcacagggcg
    56641 cgaaataagc ctcaaagacg aatcgaatac aaccgtgagt gatgccaata tcttagcgaa
    56701 tatcatccta gatttcatct ttgaacatca tattcctttc aaagaaggat atgagatttt
    56761 accagcgaat caagagtatt acttctacaa atgcattaca aaaagagtct gctgcatctg
    56821 tggcaaaaca ggagctgata tcgatcactt tgacaaagcg ctgggaagac gaaagcgcaa
    56881 agaagttgat cattcagagt acacatttgc agcactctgc agaatccatc acacagagaa
    56941 acacaaaata ggtgtgatca atttcaaaaa taaatatcaa atcaaaggga tcaagttaaa
    57001 ccaggaaaca attaaaaagt taaggatagg aggataaatt tggctgagat aagttggatc
    57061 aaacttaaaa ctactatgtt tgacgatgaa aaaatacgat taatccaagc tgttcctgag
    57121 tcggatgcca tcatcgttat atggattcga ttactagttt tagcaggaaa gactaacgac
    57181 gatggtctga tatatatcca gaggaacatg ccttataccg aagaaatgct tgctacattg
    57241 tttggcaaaa acgtaaatac ggttcgctta gcgctaacta cattggcaaa tttcaacatg
    57301 attgatctaa gcagtgatgg actaattgcc atcagtaatt gggaaaaaca tcaaaatatc
    57361 gagggtatgg ataaagtaag gctaaaaaat gctgaaagaa accgtaaata cagggaaaga
    57421 aagagacagg aacgtctcaa attggaaaat gacgttagcg tgacgtcacg tgacggtaca
    57481 gataaagata tagaagaaga taaagatata gataaagaag aaaagaaagg taagtattct
    57541 gacgaacact tacgccttgc taaaaagttg caaagtaatt taactgaaga ttttccaaaa
    57601 gaaatgaaca aagtagatat cgaaaaatgg gcagacacaa tcaggttgat ggaagaaaga
    57661 gataaagcat ctatagaagc gattgagtat gtgatcaatt ggctacctac aaatgaattt
    57721 tggtttggaa atattagaag tgctaagaaa ttgagagaaa aatttgagaa gctcaaattc
    57781 gaaatcaaag cagacaagaa gaatcataaa aagcaaagtc aaaaactaca gtacagcaat
    57841 cctagtgaat atgacgactt gccaatttaa aaaggagatg catcacatgg aaagcctagc
    57901 aaatgctatg gagaaactaa taagaagagt attagtgcaa agcggaaaat gtccagaatg
    57961 tagcgaacct ttgtatagtt ggcgagctaa aaataaggat ggttcagaac gttgtaaacc
    58021 aacatgcatg agttgtggtt ataaagcgtt acgtgtgaaa gaggatatac agaccgaacg
    58081 gatatataac gatagcttaa aagcacgagc gttgagtttt tttcaaaatg gttcggtatt
    58141 aacagataaa actttgttta aatgcaaaat ggaaaattat cacgtagtgg atcaagaaac
    58201 aaaaatcgct ttagaaaaag caaaaagcta tacgaatgag gttctgctga accatcctgc
    58261 acatttcatt ctatcaggga aatcaggaag cggaaaaagc cacttatcaa tggccacagc
    58321 ttgggaaata cttgagcgct caaattatga caagaaaata ctttttataa gctatcaaga
    58381 attattagag caaataaagt tttcttataa caatactgaa ctgagaaaag aaattgaagg
    58441 atcgcttata gccgatatta aaacaactga tttggtggtt tttgacgata ttggagctga
    58501 attaggtagt ggggtatcaa atagtaggca gtttacaaac aacacgttaa acacgctctt
    58561 agaagccaga cagaacaagg caacgatcat cacaacaaac ttatctggtc ctgaactaag
    58621 agaagcctac ggtgaaagaa ttgtttctag gatatttaag aattcagaag gttatgcgct
    58681 gaaattccaa caaacagcag acaagcgcat aaaaccagtg aaaggtagta tcgcatgaat
    58741 aaataccgta ataaaaaaac tgttcatcga ggtatcaagt ttgattctat cgcagaagca
    58801 gagtattacg atctagcctt gtggcaagct gaagcaaatg gctggaaagt aaaacttcag
    58861 gaaagatttg agctgatgcc gaaatttgaa ctagacggaa agaagtatcg caagatcgag
    58921 tatattcccg acttcacatt ttataaaaac ggcaaacttg tcaaagtcgt agatgtcaaa
    58981 ggaatgcaga caaaagactt taagatcaag gcaaagttgt tctgtcatca atatcaagcg
    59041 ccgttgatat tagctaaaaa atatcggaat acgttcaagg aagagcgttt ttaacgaggt
    59101 ggtccatcat gacaacagaa gaagtgattc aaatgcgtat tcgaaacatt cagcgtgaaa
    59161 ttgacgatct agaacgaaca aaggcagtga tggtcaatga aacggcgaga aaggcaatcg
    59221 atttgcacat agagaactta agaagggaaa ttcgtagatt ggaggaatga gcgtggataa
    59281 gaaagcgaca atgaaacgaa ttgctgaatt aaccaagtca gaatcttggc aggaagacaa
    59341 agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg tggactgaaa agcctaaacg
    59401 gaaaacgccg agaaaaattg caatctggca tggtgatcga attctagtaa caggtacagc
    59461 tgaacagtta tctgaaatta ctggattaag caaaaacatt atctgggata gagctaggag
    59521 cttatggatt gattcaaaag gacgacagtt taggtatgtg gaggagagat aatggatctc
    59581 attacacaat acagtgacat catcctcaag aaaatcatga tgaagattca gaaagacaaa
    59641 aaatcaaaag aacgagctga attagttaag ttagaaatgg ctgaaacagg agcaggagtg
    59701 cgaagtagca ggcattggaa agcagcagca aacattgaat tttattacaa cgaaattcaa
    59761 aaagggttcg atcagatgcg tgagctggat cggcaaacaa attggagcaa gaaacttcat
    59821 caagatcgtt tcaaatttgt agagaagtat aaagaaatat tagaagagta tttgaggagg
    59881 acagcaaatg ataaaaaaac tcgttcaatt cagcatggat ttatatgata tcgaatcagg
    59941 agcaacacta tctgtggaat cggaccatct aatcataaat tttggtggaa agcgccagat
    60001 tattttgtgg gtagttgatg atgtactgtt tccagaaatt gttcatgatt tcgaagaatc
    60061 aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa ttgattgaaa aatacgagga
    60121 ggacagcgaa tgatac
//
LOCUS       DASHFF010000011        59366 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.011, whole genome shotgun sequence.
ACCESSION   DASHFF010000011 DASHFF010000000
VERSION     DASHFF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.011"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(159..1469)
                     /locus_tag="U2I75_000791"
     CDS             complement(159..1469)
                     /locus_tag="U2I75_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1695943.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="U2I75_000792"
     CDS             complement(1481..2899)
                     /locus_tag="U2I75_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1695944.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="U2I75_000793"
     CDS             complement(3139..4707)
                     /locus_tag="U2I75_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1695945.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="U2I75_000794"
     CDS             complement(4711..5121)
                     /locus_tag="U2I75_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN1695946.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="U2I75_000795"
     CDS             complement(5118..6041)
                     /locus_tag="U2I75_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN1695947.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="U2I75_000796"
     CDS             complement(6038..6916)
                     /locus_tag="U2I75_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN1695948.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="U2I75_000797"
     CDS             complement(6903..8240)
                     /locus_tag="U2I75_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1695949.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="U2I75_000798"
     CDS             complement(8243..9346)
                     /locus_tag="U2I75_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1695950.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="U2I75_000799"
     CDS             complement(9420..10928)
                     /locus_tag="U2I75_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1695951.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="U2I75_000800"
     CDS             complement(10894..11586)
                     /locus_tag="U2I75_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1695952.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="U2I75_000801"
     CDS             complement(11608..12582)
                     /locus_tag="U2I75_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1695953.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIVAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="U2I75_000802"
     CDS             12882..13754
                     /locus_tag="U2I75_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1695954.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="U2I75_000803"
     CDS             complement(14212..16410)
                     /locus_tag="U2I75_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1695955.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="U2I75_000804"
     CDS             16642..17634
                     /locus_tag="U2I75_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1695956.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="U2I75_000805"
     CDS             17694..19115
                     /locus_tag="U2I75_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN1695957.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="U2I75_000806"
     CDS             19108..20607
                     /locus_tag="U2I75_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN1695958.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="U2I75_000807"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="U2I75_000807"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1695959.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="U2I75_000808"
     CDS             22199..22882
                     /locus_tag="U2I75_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN1695960.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="U2I75_000809"
     CDS             22884..23720
                     /locus_tag="U2I75_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1695961.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="U2I75_000810"
     CDS             23731..25485
                     /locus_tag="U2I75_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695962.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="U2I75_000811"
     CDS             complement(25761..26102)
                     /locus_tag="U2I75_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1695963.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="U2I75_000812"
     CDS             complement(26170..28341)
                     /locus_tag="U2I75_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1695964.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28510..29409
                     /locus_tag="U2I75_000813"
     CDS             28510..29409
                     /locus_tag="U2I75_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1695965.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="U2I75_000814"
     CDS             complement(29480..30337)
                     /locus_tag="U2I75_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1695966.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="U2I75_000815"
     CDS             complement(30322..33024)
                     /locus_tag="U2I75_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN1695967.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="U2I75_000816"
     CDS             complement(33037..34326)
                     /locus_tag="U2I75_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN1695968.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="U2I75_000817"
     CDS             34476..35522
                     /locus_tag="U2I75_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1695969.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="U2I75_000818"
     CDS             35524..37677
                     /locus_tag="U2I75_000818"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN1695970.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38150..39601)
                     /locus_tag="U2I75_000819"
     CDS             complement(38150..39601)
                     /locus_tag="U2I75_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1695971.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39598..41322)
                     /locus_tag="U2I75_000820"
     CDS             complement(39598..41322)
                     /locus_tag="U2I75_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1695972.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41315..41929)
                     /locus_tag="U2I75_000821"
     CDS             complement(41315..41929)
                     /locus_tag="U2I75_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1695973.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42274..43731)
                     /locus_tag="U2I75_000822"
     CDS             complement(42274..43731)
                     /locus_tag="U2I75_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1695974.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(43772..44692)
                     /locus_tag="U2I75_000823"
     CDS             complement(43772..44692)
                     /locus_tag="U2I75_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1695975.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44704..45651)
                     /locus_tag="U2I75_000824"
     CDS             complement(44704..45651)
                     /locus_tag="U2I75_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1695976.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46130..46849)
                     /locus_tag="U2I75_000825"
     CDS             complement(46130..46849)
                     /locus_tag="U2I75_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1695977.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(46898..48544)
                     /locus_tag="U2I75_000826"
     CDS             complement(46898..48544)
                     /locus_tag="U2I75_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1695978.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            48723..49313
                     /locus_tag="U2I75_000827"
     CDS             48723..49313
                     /locus_tag="U2I75_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1695979.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49405..50910
                     /locus_tag="U2I75_000828"
     CDS             49405..50910
                     /locus_tag="U2I75_000828"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN1695980.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(50994..52346)
                     /gene="celB"
                     /locus_tag="U2I75_000829"
     CDS             complement(50994..52346)
                     /gene="celB"
                     /locus_tag="U2I75_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1695981.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52346..52864)
                     /locus_tag="U2I75_000830"
     CDS             complement(52346..52864)
                     /locus_tag="U2I75_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695982.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(52880..53206)
                     /locus_tag="U2I75_000831"
     CDS             complement(52880..53206)
                     /locus_tag="U2I75_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN1695983.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53219..55198)
                     /locus_tag="U2I75_000832"
     CDS             complement(53219..55198)
                     /locus_tag="U2I75_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1695984.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55219..55533)
                     /locus_tag="U2I75_000833"
     CDS             complement(55219..55533)
                     /locus_tag="U2I75_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN1695985.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55701..55994
                     /locus_tag="U2I75_000834"
     CDS             55701..55994
                     /locus_tag="U2I75_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN1695986.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56072..57184)
                     /locus_tag="U2I75_000835"
     CDS             complement(56072..57184)
                     /locus_tag="U2I75_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1695987.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57571..57750
                     /locus_tag="U2I75_000836"
     CDS             57571..57750
                     /locus_tag="U2I75_000836"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1695988.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            57944..58114
                     /locus_tag="U2I75_000837"
     CDS             57944..58114
                     /locus_tag="U2I75_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1695989.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58538..59278)
                     /locus_tag="U2I75_000838"
     CDS             complement(58538..59278)
                     /locus_tag="U2I75_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1695990.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17757 a        12144 c        10387 g        19078 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ctattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt
    37861 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta
    37921 gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtgaaaatg
    37981 gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt tgaactccgt
    38041 ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa taagaacagc ccaaaattag
    38101 atttctagac aattttttat aagttatttc attacattgg ctttttcttt tatgattctc
    38161 cactgttttc tcggtactct ttaggggatc tgccgacgat tttcttgaat aatttagaga
    38221 aatacgtggt gttgttgtag ccaatctcac ttgctatctc attgatgttt tgattactgt
    38281 gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt caaataagct gaaaaacttt
    38341 ttttcgtttc ttttttgaac agctgtccta gatacatgac atttaaatgc aggttttctg
    38401 acacttcttt gagtgtaagt ggttcttgat aatgctggtg caggattacg attacttttt
    38461 ccacattgtc actgtacttt ttctgtctct gatgcttttt gatgaatgac agaagtagct
    38521 gctgaagttc ttgcaccgtt tttgcctgat ggattttttc gattccttgt aaatattcct
    38581 catcttccag atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa ctggaatatc
    38641 gtatatcttc cggcatcatg gctgctaact gaaactgttc aaaaaaatca gtgatcattt
    38701 tttgagcgac ttccaattgt ttattttgca gcacgcgtct gaaacttgaa aaatcgatgg
    38761 tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta tctccataaa
    38821 attgatgcaa ctgtagattg tctttcgctt gttggaatgt ttcagggatt ttgtccaaag
    38881 aatctgtctc ttccccgata ctgatcagcc attcctgatc aggaaagttt tcttgaacaa
    38941 aacaacagaa gccatcaact tcatctgcct ccaacaacaa cgtgatcaat acacggtctc
    39001 cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg caaccatcta ttgataagag
    39061 tatcatgagt tcttgccagt tgaatcaata ctatcctatg ctgcttctca cttatttgtc
    39121 ctattatttc ttcttcactt atttcatcca attcttcatt caaccattga ctgaataaca
    39181 gttcttgata gatttcttgt tgattttttt gttcattttg ctgatctaac cgtgttttga
    39241 ttttcttgag gctttcaacc aattcgaact tattcacggg tttcatcaaa taattcacgg
    39301 cgcctaattg catacctcct ttcaaataat cgaattcttg gtatcctgat aaaatcataa
    39361 attcaaaatt atgacgttct ttttgagccg cttcaataaa ttccagtcca tttatttctg
    39421 gcattgtcac atccgttact actaaatcaa tctgtcgctg ttcaagtaca gctagtcctt
    39481 ctttggcatt ctctgctgtt gctactattt gaaagcctaa actttgccaa tcaattatct
    39541 tttttaaccc attcaaaatc atgtattcat catctaccaa taacacttga tacacgctca
    39601 tccctccttg aatgtaatag aaacggtcag tccacctgtc tcattattct caagcgacat
    39661 ataataactg ctgccgaagc tggcacgtaa acgttcattc acgttttgca gaccgataga
    39721 accgtgaagt tccactttag gatgagacag tttttcttca atctgtttta gccgtttttc
    39781 agtcatccct tttccattat ctttgatgat gatccttacc cgtttgcctt cctgcaagac
    39841 tttgacactt agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa aataattttc
    39901 aactaatggc tggatcacaa atttaggtac ttcgatcttt tccaaatcag gatcgatcat
    39961 aaaatgatag gctactcgat taggataacg catttgatac aaatagacat atttttcgca
    40021 aaagtccagt tcttctttta aagtgatcgt tttctcttga ttcgtattgt ttcgaagcaa
    40081 agcggaaaac gcatacacaa catctgctaa ttcttcactt ccttcgctga ttgcatacat
    40141 tcggatatat tctaatgtat tatataaaaa gtgtggattg atctgtgctt gaagcgcccg
    40201 catatgtgcg tcttgctgtt tgatttctaa tttgtagatg tcttctacaa actgattgat
    40261 gctatccaac atttcgttga taccgattga aagttccttc agttcatatt ccgtgtttgt
    40321 tatgtcgatc cgcgtctcta aattccctgc tgctgtttga tccatggcct gcatgacgat
    40381 agatacttgc tgtgaatatc gtttgaatgt tttataaaga aagataagca gtatattgtc
    40441 taaaataatg ataccaatga tcagcggggc aagatttttt cccgtttcga tttttacact
    40501 ttttttatct agtacagcta atatacgata atcatcagac agttcttggt gttgaacaaa
    40561 attgtttttt tccagatcgt caatcggcaa ttgtgaattt ttcttcagac tctctgtgat
    40621 gatcatttct tgtctttcaa acgtttcttt agctatttta ttatcatgga atgtgtatag
    40681 tcgatttgtc gttccagaaa tcatgtataa agagagtcca ttgaatgacg ttagattttc
    40741 taaaatcata tctagttctt tcttttgaaa accaataaaa atacttcctg actcagctcc
    40801 tccttgtgtg ataggcaact tgatataaaa ggcgtctagt aattttggtg tccccttttt
    40861 tatttcccca cctttgtttt ctctagaaga ttcataatac tccggtagtt gattcaacac
    40921 gatatatatc gcacttagtt cttcataatt tgcgtagaga tctttgattt gatttgggaa
    40981 agaaactaat tcatcggtac tgttttgctg ttcgtataca tacatcagat actgatctat
    41041 gggttttgtt aagtaggtcg tcacattttc gatcttttcg gaattcccta caagctggtt
    41101 tttgatgatc ctactcaagg ttgttttatc attcagcatt ctgcttgtgc tttgaacggc
    41161 atcacttgtc atgttttctg cctgtttttg attttgttcc caagtatgga tactaaaaat
    41221 aacggtagca gtcatcgtta caccgatcat gataaccgcg tatttccgca acagacgatt
    41281 tagtaaggtt ccttttgaaa atagaatccg aaacttattc ataactttcc aactgtttat
    41341 ctaattcttc acgccaatgt gttgtcatcg taccattcca aaccgtcaaa agaccaatga
    41401 caccaaacag gatcaatcct ttgaattgcc aagtcacgcc taaaattata ccgctaccaa
    41461 taattatttt tagaaaagac gaaaagctgg aaaaaaccga aataaaactt agctttagca
    41521 gattaggaac agagatcgta tattcactat ctaaaatcat actgtattga taagtagcaa
    41581 ataggcagag caaggcaaaa agtaaaataa aatcaatagc taaaaaaatg agccctttga
    41641 tttgaacaga taaatataaa ttgtaactca ataaagcagt accagataaa aataaactaa
    41701 atagcagatt gcctcttaaa aaatttcttt tatatatatt ccagcctctt tttattgtga
    41761 tttctttata ctcgaatccg tacaaataaa atagttcatt gactgttttc catgccgcac
    41821 ctaatccaaa gatgatcccg ccggcaaatg tatatatcca gaaatacaag gttagtttta
    41881 taatgaccca gatccggata aataactctt ccaaaacctt ccctaacata tcttctcctc
    41941 caaacattaa gtttatactt tctcattata caaaaaaagg ttgggcaatc agcaccttga
    42001 cctgattatt agagaaacaa acggaaaaat agttcctcat attttgtcgt tttgtgagcg
    42061 aataccgagg gttagctgat tgaacaccgt ttaccaagag tttgtgaaat agccgcttag
    42121 tttcgagtag taagaaaaat tttgtgaata tggctttcca catttttaca tatatttagc
    42181 ttaataccga gaaactggct tttgaacacc gtttattcgg aacaagaggt tgtgacacgt
    42241 cttttgtcac aacccctttg tctttattca ttattattct tgtcggaact catcatattg
    42301 tttttgcatt tctttcaata ccttatcata gccagccttg tccaaagcgt ctttcaattt
    42361 cggtaatgtt tcatctggat caacagtccc tgtattcaat ccatctaaat attgattcat
    42421 gacattagaa atattactta attctgtttt gacactgtca gtgttgaagc tgaatcctaa
    42481 aatcggtgaa gtttctgcat cagcaatcga ttgatcacgt ttggcaatca tgtcatccgt
    42541 aatagactct tgtgtatata gaatcttgtt gtttcctgta ttccaagcag acatatgtgt
    42601 atttggctga taaccatcta gcaatttgat tttatcatct gaaccatcga ctttttccca
    42661 tgcttctccc tcgatccccc aaacaagccc attcaataat tctggatcac tgttaagcag
    42721 ccctagaagt tctactgatt tttctttatt ttttgaagta tttgatacga caaaatttgc
    42781 catttgtgct tgagctgtag attttagtgg aacagtaatt gcttttgaga caagttcttg
    42841 ttttgctgca tttgtcaaaa ttgtatcgcc ataatcatac ggtccttgtg tttctccacg
    42901 cattagccaa gtatttcctt ctagtggata atccttattg ctcgttgcag cgtcacttgg
    42961 aatgagtcct tgtttgtacc agctatgcat tgtacgtaac agtccgatat acgtttcatt
    43021 atcgtattgg ttgatgattt tcgaatcatc tccatccaga tcaatagcaa atggtaacgt
    43081 attccctaaa ggataatcaa agttcccttg gactttgaag ccttgtccaa tggcaaaagc
    43141 tgccgtgttt ggttcttttt catggaattc cttcaagact ttttctgcat cagcgtaaga
    43201 ttggattcca tcaattgaaa gatcgtattt atctagtaaa gctttgttaa aagtaagtac
    43261 ttgttgagca aagacatttc cattgactgg gaaagcatat aattttccat tgaccagatt
    43321 tcctttaata tatgcttcat ctaaatcatt ataagctttt tcagcgtact taggcgctaa
    43381 ttcagtcaaa tcagcaaagg ctcctttttg ggcattggaa acatagttgt tcgcaaaggc
    43441 gatatcataa ttttccccgg aagaaatgat gacgttcatt ttcttttcgt aatcgcccca
    43501 gccaatgtaa ttgatattga cttttacacc gatctctttt tcgattcgtt tgtttgctac
    43561 ttccatcaat tgatcgaaat tatctggttt atctccgatt tgatacatct gcaaagtgtc
    43621 cgaattgcta gcttctgcct ggtctttaga accagaacct ccgcatgctg ctaatgttcc
    43681 caacaacaac gctgaaacac ctgtaaaggc tagtttcttc cataacttca tttttcttcc
    43741 tcctaatttt atgatttata tttttgtttc tttattcttt tactccgccg attgttagac
    43801 ctttgacaaa atgcttctgg aaaaatggat agctgatcgc aataggcaac gtcgagatga
    43861 ctacgatcgc cattctggca gattctcctg gaactgaggc taatccgcct gtcaattggc
    43921 ttcctgctcc tgaattttgt gtcagatatt gaatattact ttggattttc atcaaaagat
    43981 actgtagtgg aactagctta tcatcttgga tgtatagtaa agcattgaac cagtcattcc
    44041 agtaacccaa tgcggcaaat aaactgattg tcgcaatacc tggaacagct aacggtaaaa
    44101 cgatctgaac aaatattcgc agttcgctag ctccatcgat ccgtgccgat tcaataatcg
    44161 aatcagagac cgaacgttta aagaatgtgc gcatcacaat aatattgaag ggactgacag
    44221 ccattggcaa gatcaatgcc caaatcgtat cttttaactg aagaagattt gtcataacaa
    44281 gataattggc caccatccct ggtgagaaca acatcgtaat caaacaaaaa acggtaaaaa
    44341 aacgacggaa tggaaagttt gaccgtgaaa tcgcataagc atataaagaa gtagccgtgc
    44401 tattgatcag tgtacctaaa acagtaacaa gtacagtgac gaaaagcgct tggaaaattt
    44461 tatcctgcat ctggtcgaac aagtacgtat aaccaaatat actgaacttt tctggccaaa
    44521 aactgtaccc gtgagtcgac aatatggact catcggtaaa agagatggcg ataacaaata
    44581 taaatggcaa aatacaggaa atagcaaaga gtgcaatcat tatattagag aaaaaattag
    44641 ccgtcttatt gaaagagcgg acttggactc tctccacttt cttttttttc atttttttcc
    44701 tccttagaat aatgcagaat cgctatcaaa acgtcgggcg atcgtatttg tgatcatcaa
    44761 caagacaaag ccgacaactg attggtaaag tcctgcagca gccgtcatac caaaatctcc
    44821 tgtggatgtc aggccattgt agatatacgt atccaaaact tgagtcacat catataatgc
    44881 accggaattc ctaggaatgt tataaaacag gccaaaatca gctcggaaaa tgtttcctac
    44941 ggctaaaatc gttaaaatcg tcattagtgg aataagttgt ggtatcgtca catgtttgat
    45001 ctgctgccat ttgctagctc catcaaccat agcagcttca taataagtcg gatcgattcc
    45061 cataacagat gcaaagtaaa taatgctgtt ataaccaatc cctttccatg taccaatgaa
    45121 caccaagata aacggccaat atttcggttc attataccaa ttgacagctt ctcctccatg
    45181 tccagtgatc cactgattga atattccttt gtcaggactt aaaaaagcgt aaacaaagta
    45241 actgataatg acccaagata gaaaataagg tagtaaggac atcgtttgat aaactttaac
    45301 gagtcgttta tttctcaact cgctcataat gattgcaaat acgactgaga tcagcagatt
    45361 caaaaggata aatgctacat tatataaaat tgtattttta gtgatgagaa atgcttgatc
    45421 cgaagaaaat aaaaatttga aattttccca acccacccaa ggactttccc tcaagctagc
    45481 caagaaccca tctgctgaaa tatgaaaatt tttgaatgct acgacattcg ccattacagg
    45541 tatgtaaaag aaaaaaatca tccaaagcat acccggtaca gccatcagga caagtgcttt
    45601 ataacgccat agattcataa aaaaatgatt gaaccccttt cgttttttca tctaaaaacc
    45661 cctttcgatt cattcgcaac gtaattatag cgctttcaat aatgagaata aataaacaaa
    45721 gtatagggaa aatgaaacaa agtataggag aggttgtgaa atcagcgttt tggcctgagt
    45781 attagagaaa aaaacgggaa aatagttcct catattttgc cgttttttga gctaataccg
    45841 agggtcagct gattgaacac cgtttatcag aggttgtgaa ttcagcgttt tggcctgagt
    45901 attagggaga tcgtgtcctt actatctaac tctgagtcac aaggaacaat ggggactgta
    45961 acaaaagtct tgtcacagtc ccttattccg aacaaacggt gttccagaag tagcttctac
    46021 ggaaataagc cgaaatttca caaaaaagcc atccgaaagt cagacggaca agtctgcttt
    46081 tcagatggcg ctacaactac tctgcttatt cttttagcta tccattcaat tatttttctc
    46141 tcacttgttc tagctcttct ttggtcatgg gtcgatattg ccctttattc aaatctttag
    46201 gcagccaaaa ttttcccata cggattcttt ttaaatataa gacttctttt cctactgctt
    46261 tgaccattcg cttaacttgg tgaaactttc cttcatgcaa aatcagacgg ataattgaag
    46321 tttctgtctc ttgatctact tgatcaattt ggagttttgc cggcaatgtc tgctcatcgc
    46381 catcaataat cagtccttct gcaaataaac ggatatcttc ttctgtcatt ataccgctaa
    46441 cctcagctag atattctttc tccacatgtt ttttaggtga tagtaactga tgggcaaaca
    46501 ccccgtcatt cgttaaaatc aagaggccct ctgtgtcttt atctaatcgg ccgactggaa
    46561 aaagatcttc ccgataatcc tggtcttcca ataaatcgat tacggtttga tcatgattat
    46621 cctgagtagc agaaatgact cctgcaggtt tatgcaaaat gtagtaaaaa tctttttgat
    46681 acgtaatgat ttctcctaaa taagtaaccg catcttcgaa ttcatttact tgcgctttat
    46741 cgcttttcac cacttgtcca ttgacttcga tcgttccttt tttgattaat cctttgactt
    46801 ctttgcggct gccaagtccc atatctgcta aaaacttatc taaacgcatg cgtttccccc
    46861 atttcaaaag agcatcaatc tagctcttct gattcactta ttttattttt aatttatgtc
    46921 gtaaacgcgc tattcctggt cctagtagtc tttctgccaa gcggattttc aaagctaaat
    46981 aaccatacac taaagcccct atacctgcta ctacaagaat caacaataga gactggaatt
    47041 tgctatcttc gtttaaaaaa gcaccaaaaa tcatctttgt gatccacgaa gccaaaagca
    47101 taatcaaggt aagaataaag atcagcaatg tcctgcgcca gacaaaactt ggtttaaagc
    47161 gggctacttc atggattctc tttaagatca aggtacaaga cacagcaaat ccgatcattg
    47221 tcgccaataa agggccgtat acttcaaata aacgaatggc tgggtattga agaaggagtt
    47281 tcactagaaa cccaataacc aaataattaa ttgccgcctt gttttcaaac attccttgca
    47341 gcatgttgga aactagcata tatagtccta ggaagagtcc tacaaaactt gcttggatca
    47401 gtacattact tcctagagaa tccggtgtat aaaataatgt atttagcgga taggcaagta
    47461 acataacacc aaatgtcgcc ggaaacataa aaaatgaaaa caactgtaaa tttccgcttg
    47521 tcaattttgc cagacctact cgatctttca acgtaacagc ttctgtaatc aacggtaacc
    47581 cagtagacgc gatcgatgtt gccaatgcaa taactaccat cgtcaactta tctggattgg
    47641 cactaaaaat cgaaaaaaga tccagtaact gtgcattgga atactcggtg gatgcagaca
    47701 tgatcttcat aaaagtgaac tgatcgacta atttgaagat cgttacccct gaacccacaa
    47761 taataaacgg aatcgcttct ttaacggtat ctaacaacaa ttccttcgtt gcaatattta
    47821 ttttgttttc gctgtagtga ataaacgccg atgtatacgc ttgatgtttc ttcaaaaaat
    47881 acaaaaggac aagaatactt gctagcatac cgataaatgc cgcgaaagtt gattgcgtga
    47941 ccgccgtcac atagtcacca tccatgactt tcatgatgat gaatgtagcc aatagcatat
    48001 aaaaaatacg tgccacctgt tctacaatct gcgacagcgc ataaggcatc atttcttgat
    48061 tgccttgcaa ataccctcgt atcacactca tacaaggaaa aaccaataca gcgatactca
    48121 aagaacgcat cgttggaatc agttcttctc cgccaccaga agcatgagct agccaaggag
    48181 aggcgatgta catgaacaaa gcaaacaaca ggcctaaccc gcccatcatc tgtaatgcac
    48241 ggataaataa tcgtcgtgaa gtgccatatt cattcaatga attatagcgt gcagtttgtt
    48301 ttgcaatcgc agcaggtatt cctgctgtag agatcagtag aaacaatgca tatatattgt
    48361 atcccatatt gaataatcca ttggcagcct tagcattttc gcccatccaa gcataccagg
    48421 gaatgatata gacagcgcct agcaatcttg aaatgatatt actggcggtc atccaagcag
    48481 agcctcgtgc cattttttct tgcggtgtta attgatctaa tgatggtttt ctttgattat
    48541 ccatcggtca gattccaact ttctacaaaa cattactatc attttaattg gtaaaacaga
    48601 aactggcaat cgattttata taacttttat aagaacaatt gtttttttga attcggtttc
    48661 gtttttttgg taaagtaaag aaatatacag ccttttatgg ctaacattta taggagatat
    48721 caatgacaca agaaataaaa aaactacccg ttatattttt aggtcttttc tttatcacag
    48781 ttggattgaa ttggttcttg ctccctcacg atatagcttc cgccggagtg ggcgccatcg
    48841 gacatttagt tgaaacggcc ttttctatcc aacgctcagt tactgtttgg agcatcaatc
    48901 ttatgatgct gcttctagcc gctgtcctat taggaaaatc agtatttttt aagactgtag
    48961 cgggaagtct gctttttcct gttgttttag agattgttcc aaaagtggaa atgatctcct
    49021 cacatttctt ttcactgatt tcaggaagtc ttttgttcag tcttggggtt tacacattgt
    49081 acacagtcgg ggcctcaaat ggcggtataa cgataccgcc gattattttc cacaaatttt
    49141 ttcggcttcc aatgccgcaa ggacttttac ttaccaatag tcttatcgtc tttttgaatt
    49201 attatgtttt tggattgttg gagacactat tcgttctcct atctattaca gtcagttcat
    49261 tgtttatgaa aatactcatc cgcttgtccc ctgtgtcaag aaaatttgaa taaagctgga
    49321 ttttgtcata gcggaaacaa acgatatatt aaaaagagtg tgctataata aaccaagaaa
    49381 ttgaataaag cgagggatca atcaatgagc ttctctttag aagacatccg tcatttacta
    49441 ttaaaagaaa atctattaaa agaatttgtt tcaacaagag gctggcatct tgatgtacca
    49501 gaaaaagctg aatttcttca gttaagttat gattcaagaa aagctgatgc ttctactttg
    49561 tttttctgta aaggaatgaa tttcaaagaa gaatatttgg attcagcaat cgaacagggt
    49621 attcaatatt atgtatcaga acaaccatac gaaaattcag cagtaggaat catcgtaacg
    49681 gatatccgta aagcaatggc gcttcttgct atggcttttt acgactatcc gcaaaataaa
    49741 ttgaaagtga tcggattcac tggaacaaaa ggtaaaacaa ctgctgcgta ctttacaaaa
    49801 gcaatcttgg atcacacaac aaataaaaaa acagcattgc tttctactat gaatacaacg
    49861 ttagatggca aaacatattt caaatcccat ttgacaacgc ctgagtcatt agatttatat
    49921 cgtatgatgt cagaagcagt tgaaaatggt atgactcatc tagtgatgga agtttcttca
    49981 caagcttaca aaacacaacg cgtatacggc ttgactttag atgtcggaat cttcttgaat
    50041 atctcgcctg accatatcag tccgatcgaa catccgacat ttgatgacta tttctattgc
    50101 aagcgacaat tgatcttgaa ttcaaaaaca gttgttttaa atcatgaaag tgactatttt
    50161 gatttactta gggaaacagc cgatctattc cagattccaa cgatcactta tggtcgttcg
    50221 gaagaaagca attatcaagt gatccgttca gataaaggta ctcacggttt tactttatct
    50281 tctcatgaag atcgcttagc tatctgcgat actgcttatg atattttgct ggctggagga
    50341 ttcaaccaag aaaatgcagc cagtgcgatc attgccgcag cactaacagg tgcctccagt
    50401 caagatgcac aagacggatt aaaagaagct cgtgtaccag gccgaatgga tcaattgatc
    50461 caatccaatg gagcgcatgt ctatgtggac tatgcacata attatctaag cttgaaaaca
    50521 ttgctgaaat ttgccaaaaa tgaacatcca gatggccgtg taattgttgt attaggcagc
    50581 ccaggaaaca aagcaatctc ccgtcgacat gattttggaa aagtgctatc agaaacagct
    50641 gacatcgcct ttttgacggc agatgaccca gcatttgaag atccgaaaaa aatcgcggaa
    50701 gaaatcaatg aagcaattac taatccagat ttgatcgttc attatgaaat ggatcgtcct
    50761 gaagcgattc gacgcgcttt agcagaaagc gctccgcaag attcagtcgt tattgcggga
    50821 aaaggcgtgg atccctatca aaaaatcaat ggtgtagatg aaccttatga aggggattat
    50881 gcaatcgtga agcggttgat tgaagaataa tggtatttat atactagtgg tttcccattt
    50941 ctaagaaaaa aagggactgt gattttttgt cacagttcct tttttcttag aaatcatttt
    51001 aacttttcac tagctaattc tgctgccttt tctctttctt catttaataa tttattatca
    51061 tagaatttga taaatgggaa ccagataatg aatgcgatga ttgcacaaat gactgctaac
    51121 acagcagctt tccaatcacc accactacca acaaatgctc cgatcccgac tggtgaaggc
    51181 cacggcatct gtgcaagcat cggtttgacg atttccagcc gaatggcaaa ataagcgagt
    51241 gtagcagaag ccatcggagc taagaaaaac ggaaccgcta aatagggatt gtagacgatt
    51301 ggcatcccga aaatgattgg ttcattgata ttgaataatc ctggtacaag tgaggctttt
    51361 cctaacactt tcagctgatc agatttcgcg aggaatgcaa taaagatgat caatcctaaa
    51421 gtcgcacctg atccaccaac ggttacataa gaattgttga attctcctgc caacggataa
    51481 tttgcgcctt gtgcgttagc tgccatatta gctaatacga tcggggtcaa gaaagaagtg
    51541 atgatattcg ctccatggat ccctacgatc cacaatgcat ggatcaagaa ataaatcacc
    51601 atgacaccaa gccatgtatt cgtcaactgc gtaacaaacc caaatgggat agcaatcatt
    51661 ttgaagatat ccgttcctag tgcaactaat aatccattga tgatcatcac cacaaacgca
    51721 atgacaaatg ttgggatcag agcagtgaaa gaacgagaaa ctcctggcgg aacaacatct
    51781 ggcattttta tgacccaatt tctcttgaca cacatacaat acaattcgac cgcaataatc
    51841 gacataataa tagctgtaaa gattcctgaa gttcctaaac gttcgacaaa agagcccatc
    51901 ctaataccat ttactacagt ttctgtctca ttgatagaag caatcaagct gattttacca
    51961 tcagtcatga tcaattcagg gatacacata aagaatgcaa aaacagatag taatgccccc
    52021 gtgagcggat taacatttaa tttttgttca tctcgttcaa ttgacgttaa ttcataagca
    52081 aacacaatgg caaagtaaat agataaaatc cccatcgtca ttgtattgac gatcatgtac
    52141 aagtcactaa ctttgaaaaa agttgcatta aaaaatcctt ctaaaaatgg gaacggtaat
    52201 ggcaaaatat ttaaaaccaa aaacatcgag cctacaatcg tgaaaggaat agaagccatc
    52261 cccgctgcca tgacagcccg aacgatttta agttgcgcaa ttttccccat aggtcccatc
    52321 aggtatttct gcaaaaaatc aaacattagt tattctcctt tcctaaatat aacgcttttt
    52381 catattgttt aatacggata tactgttggt cttggttgag tgaaattttc tttaaacgat
    52441 actgtataaa tgcactggca aaaataccgc ttacaacaag aatgaaaaca aataactgtc
    52501 ctccattgtt atttgccata aaaggataca agaatgtaaa tcctgaggta aacaacactg
    52561 gaatcactaa taaattaaag acactctgga acaaaaaata ccactttgtc catggaacgt
    52621 tcgtttggtg ttttcgataa agctttactt gttctcctac tgcaggcaaa atagctaaaa
    52681 tcagaatcat cggtaaacta cttcctaaac ttttagctga tagaagcaga atcatccatt
    52741 gcatgttcat gaaaaagaaa aaggcagtca ggtaacgtac gattagataa cgattgaaat
    52801 acatgttttt caaactaagt tgcttttttt ctaattcgat tttccttttt actgtatcct
    52861 gcacaatgat ccttcctttc tatttatttt tttgatacaa ataattcatt tcctgtgcaa
    52921 cttccaataa tgtcatcgtc gtcatcagat gatcttgtgc atggaccatg ataatttcca
    52981 tttctatttt tgtcccgctg gcatattctt tcagaagttg agtttgtgac tgatgcgcat
    53041 gaagaatctc ttcatttgct tgtcgcaact tctcttctac ttcttcaaac atctctttac
    53101 gcatcgagcg aaatgcttca tgtatcagtg tccgtgcatt tccgctatgg aaaatgatat
    53161 caaaagaagt cacttgtagt gtttcactgt ttattttttc agccatctgt tttcctcctt
    53221 attcgatcag tttgataaat ttactgctga aattctcaaa aataggttca gctaagattt
    53281 cctgctgtac ttttagctgg tcaactaacc taacgattgc tttagtcacg accgtaattc
    53341 cttcattttc gacataagaa ggcgaaacta aaaaaacaaa atgaatttct tgatattgat
    53401 cccactgcat cccagaagga atcaatgcaa cggctacctt tgtttcgatt ccaactggaa
    53461 cagcgggatg tgggactgcc actgtatcac tgaatatgat ttgtcccata ttttcacgtt
    53521 gctggatctg ctttcgcatt tctcttatat atccttcttc ttcatttatg ctcagctttt
    53581 gcagcaaatc atgaagaatt tcttcctttt tcggttcgta cgtataagtc ttgaaaaatt
    53641 tttcagacaa ctgttcggtg taaatctgct ttttttgctg tagagaaaat gctggtatcg
    53701 gtctatctga ggaatgatag ggtatagatt ctccaactac ctttcgtatt ttacgaacat
    53761 catcttcatt caaaaaaata ctgacatgta aaactggaat tttgaaaaac attgtcgaaa
    53821 gatcaataga agaaataatc aggtcgattc cttttaaagt tgtttcattg atctcataat
    53881 agccttttac acttaccact gtaatatttt taccgaattc actgactacg cgattcttta
    53941 atagttgggc actgccgtaa cccgttgcac agataatcaa tgcatgtagt ttatgcgcag
    54001 ctctttcttt ttccaatgcc gccatgagat gcaaagccaa ataggcccat tcatcttcat
    54061 tgatggtata gtttttcaaa gccggcatgt tgctgaaatg cctttttact gcgtcaaaga
    54121 ctttgggatt ttctttcttt atctcctcag tcaatgggtt ctcaatgcga atacctcggc
    54181 tcaatcgaat catcatcggt gacaaatgat cgagtagtcc attgaaaagc tggagatcct
    54241 ctactaatga aaaatgaaat tcctgagaca atcttttcag aactttcgct aattcctttg
    54301 atagttcctg atcattttga ttttctttgt gattcgactt agccatgaga tgtaatgtca
    54361 aataagcaat ttcttcttcg ggaaacgata cacctgcgat tacctcaata cgttgaacga
    54421 tccgctgcgc cacttgatac tctactcgct tagaaacttc ttgttcgatg cctaattcct
    54481 tgatttcaaa cccttctcgg agccgtttga tccccaaagc caagtgtaaa gtcagattct
    54541 gtatgataaa atccgaaact tttaacttag cttctcttgt ttcatcgaga atgataatga
    54601 ctagctcttc aaaactgatg tctgggaaaa aacggctttg ccctaaatat tctttcaatg
    54661 aatttccata atggtttcca aaaaaagtat ccatgataaa atgccgtcga tttctttctt
    54721 ccccttcgat ccatgtccct tgaccatgct tagaaacgat ttttagtgaa tatggaatca
    54781 atcgctcctt gatttcttgc atgtcctttg tcaaactgga acgactgata taaagctctt
    54841 ctgccaaatc atccataaaa agaatggcat cttccaaaag caattggttc aatatatact
    54901 tttgtcgatc ttcggactca taaaatcctt gcggttctct agcatatcca ggtactattt
    54961 cttgccgact caaaaacaag tcaaatgtca gtctttgtaa aatatgcagt tggtagccat
    55021 aacctggttt agcaatgatc ttacctccgt ttttttctac taactctttg atccgctgca
    55081 agtaattacg gattgtccga tcagataata acaattcaga agctaaacgt tgactggtga
    55141 catactcctt cttgtgatca atcaaatatc tcaacagcaa tttttccttc tgtttcatta
    55201 tcatccactc ctattccatc atatgttctc ttcgatcacc tctgctaact tctggatgcc
    55261 tgtagggatt gggacatacg cttggaaagg gatactaaca acaggctttc caacagattc
    55321 tcctaatttt ttgaatttat ccaaaaacat cgtcgtttgc gggctgatca gaaataaatc
    55381 aaaatcacta ttcttaatca tattatcgcc ttctgtagct gaaactgcat ctacgagtac
    55441 ctcttctctt ttttgcttaa agaaatccgt tgtctttgag gccatcatcg aggatgacat
    55501 tcctgccgca caaataatca atgctttttt cataaatcaa tcactccttt tatttttata
    55561 aaccaagcat atcttgtaag cgttttctat tcaaaatata tatttccgta gcattgcgga
    55621 aaaaatgtgt atcatatctt gaataataat ttctggcacc gtgatatact agtgatagaa
    55681 ttttttcagg aggggattta gtgagtatca cagtgaatat tttgtataca agtacaaatg
    55741 gacaagccag aaaatttgca gaagaaatgg tcgaaagaaa cattgtcaaa gcgattcgtg
    55801 cagaagaagg aaacgagaaa tatgactatt actttccact agaagatcca gaatctcttt
    55861 tactgatcga ccgttggaaa gatgaagaag caatcgaaaa gcatcacaaa tccgaaatga
    55921 tggctcaaat aaacgaactc cgtaaaaagt ataaattaaa aatgaaagtc gaacgtttca
    55981 ctgatttacc gtaaaaagat acacatggaa tgatagcaat ttatcatcca tgtgtatctt
    56041 cactttttcc ttatttttta ttcgatctca tctaatgatt caaaccgtgt ttttctctta
    56101 tttttcggat aagtatttca gtcaccccaa tatacatgat tcctaaacat accccaagga
    56161 tattctcaaa aatgcggatc gttactgctc ctggaacccc gaataaactg gcagtgattg
    56221 ccaaagcacc aaaagaatta aaaactgttt tatatctata ggtagaacaa acacctaaag
    56281 ctagaccacc taatataccg acccagttaa aaggaataaa ttggctgata ccaataaata
    56341 aaacacatcc aatcaatgag ccaatgattc ggtctactgc tcgttctttg aaaccaatcg
    56401 acatcaatcc atagctagat acaatagaag aaaatgcaaa ccctgcccac atcaatcgct
    56461 gaaaagggac atactctcct gcaacgatca aaagactaat gccaaatgca tagatgagca
    56521 tccaaatttt ttcctgcgaa tacatgcctt tcaaaaaaat ctctttaaac aagcttttgt
    56581 ctttgttctt ttctcgatgt tttctgtaaa ggagaactga aaaccaacaa aaaaacaaaa
    56641 ataataagct acttgtttgt gtaaagaaat tcttgttcaa cgaatcttta ggtaacgaat
    56701 aaacaataaa gagatatgaa aagccgtaca aagaagcatt ccccattttg ggatcactag
    56761 cggttgccat taatatgctg cttagtaaaa taaaatgaat caacatgccc ataactgacc
    56821 atttgatcag ctggacatac ggtgctacta atagtgagaa catgacgatt gctaaaccta
    56881 tgatcgaatg ggaaatctta taaccaaagt caacaaaacg aatactcatc aacatacaga
    56941 ataaaaccac agcgtatggt ttatgcttcg attcaaataa tgttgtaatt ataatgataa
    57001 aacaaatggc aaacaatacg atgaataatg atcgtaaaaa caatgaacaa atatacatgc
    57061 acttttcttt tttagaagct gcttctcgta ttttttgttt taaaataaac ggatctagtt
    57121 gtagagactg ataaaaatcc acctgttgtc gtcctttctt tttactttta ttttttacag
    57181 tcatctatga caggttgtaa atactatgga acatcatatg gcgttgtcat tttcttgtca
    57241 actatttttc ctattcatgt ttttataaaa aaacatatcg ttttcttgta tgattaaatc
    57301 accacaaaat aaacaactga gagaacgata tgctttatga atggttctat caaaaaaatt
    57361 ctgagatcaa tagacaaaaa tttgatgatt acagaggtct cttacgaaac cttacagaag
    57421 aaaaaaacat tgatcgttgc tgcagttctc ttccctgctc catgtgtctg tagaaattgt
    57481 ggttctgctg tggtagatgg aaataggaaa ttaatggtgg tgaaaaacaa gaagaaggaa
    57541 accattgtcc actttgaata atataatcat atgccaatgg ttatacgcct aaaaaagcag
    57601 cgctatacct gtaaaaactg tcgaagccat tggaacgctc aaagttattt tatccgtcca
    57661 aggcactcga tttcaaatca tgtcagacat aaaatcactt ctttactgac tgaaaaagtg
    57721 tcgttatttt ttatttcgaa aagttgttag gtatctttga caaccgttat tcgcacattg
    57781 aaagagttga gaagctattt accaaagtct tccaaaagaa ttctgtcttg agtgttgagg
    57841 gttgtgaatt tcgttctcac gtttccgttg aggataaact gagttttatt tccgcggatg
    57901 gcgagacaga aaaattaatc gagaaaattt acctacacaa aaaattcctc gattaacaaa
    57961 ctatttcctt agctgcacta atcctaaaga aatagaattc ttagttacag acatgaacgc
    58021 cacctacttt cagctcacca aacatgttct accaaatgcg aaattggtga ttgatcgatt
    58081 acttggtttt tccacgcctt tcaaggaggc gtaaccttta ttcacgagtt ggttgaagct
    58141 ttccgagaca aaagacccga cttgtttttc tccttattga caaagagcca ttaaaactag
    58201 agaaaaaatt acaactgatc gctgattatt tgtagaaaga aaatcaaagt attacccaat
    58261 aaacttgagt cattaggcaa aaaacactgc gaaactcaag aatgattttc gcagtgtttt
    58321 tgtatatttt ttattaacta gtattactac aaaggtttat ttttcacttc tcaacgcagc
    58381 acaaaattgt ctttacttat ttactggttg ggttcaacct tctctactcc gatactaata
    58441 tttgtttgta taccccttct aaaatcggta gtttccattc aattgatggg tactagttga
    58501 ttttaaaaac tatgcttatt caatttttta ttttagctca tatatttcgt ttctttctaa
    58561 attttatact gatgaaaaca agtagcacac ccaatattga caatataaac tggctaatga
    58621 tggtttctcc agtttttgga agatgcttat ttccactgct tgttggattg tttcctctta
    58681 tatctgagct atcagcaact tttctattct cttttgttaa aaagatcgcc acttcttgtt
    58741 ctttttcgat cttgaaagtt actggtgtcg catccaattc ataccccgtt ggcgcttgag
    58801 tttctaccag ctgatatgct cctggtgcta agccatctat cgccagtttt ccatctgctc
    58861 ctgtggttag acctgactgc agaacggttc cattggcatc ttgtaactca aaaacagctc
    58921 cttgcaggac ttctccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa
    58981 gtcgattttc tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga
    59041 ttggtgtcgc atccaattca taccccgttg gcgcttgggt ttctaccagc tgatacactc
    59101 ctggtgctaa gccgtcaatc gccagttttc catctgctcc tgtggttaga cctgactgca
    59161 gaacggttcc attggcatct tgtaactcaa aaacagctcc ttgcaggact tccccgcttt
    59221 ggtcatcgat ctttgtcaac acgacaccgc ctggtgtaag tcggtttgct ttcgtcaatt
    59281 ctaccaccgc tgtctgactt ctttcgatct caaactcgat cggtgtcgca tctaattcat
    59341 accccgttgg cgcttgagtt tctacc
//
LOCUS       DASHFF010000012        56381 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.012, whole genome shotgun sequence.
ACCESSION   DASHFF010000012 DASHFF010000000
VERSION     DASHFF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.012"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            328..1173
                     /locus_tag="U2I75_000839"
     CDS             328..1173
                     /locus_tag="U2I75_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1695991.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1454..2674
                     /locus_tag="U2I75_000840"
     CDS             1454..2674
                     /locus_tag="U2I75_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN1695992.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2671..5391
                     /locus_tag="U2I75_000841"
     CDS             2671..5391
                     /locus_tag="U2I75_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1695993.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5375..6319
                     /locus_tag="U2I75_000842"
     CDS             5375..6319
                     /locus_tag="U2I75_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1695994.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6388..7398)
                     /locus_tag="U2I75_000843"
     CDS             complement(6388..7398)
                     /locus_tag="U2I75_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1695995.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7578..7922)
                     /locus_tag="U2I75_000844"
     CDS             complement(7578..7922)
                     /locus_tag="U2I75_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1695996.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7915..8349)
                     /locus_tag="U2I75_000845"
     CDS             complement(7915..8349)
                     /locus_tag="U2I75_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN1695997.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8487..9224
                     /locus_tag="U2I75_000846"
     CDS             8487..9224
                     /locus_tag="U2I75_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1695998.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9217..10428
                     /locus_tag="U2I75_000847"
     CDS             9217..10428
                     /locus_tag="U2I75_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1695999.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10632..11414
                     /locus_tag="U2I75_000848"
     CDS             10632..11414
                     /locus_tag="U2I75_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN1696000.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11484..12146
                     /gene="trmB"
                     /locus_tag="U2I75_000849"
     CDS             11484..12146
                     /gene="trmB"
                     /locus_tag="U2I75_000849"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN1696001.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12244..13998)
                     /gene="ade"
                     /locus_tag="U2I75_000850"
     CDS             complement(12244..13998)
                     /gene="ade"
                     /locus_tag="U2I75_000850"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN1696002.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14130..15059
                     /gene="tadA"
                     /locus_tag="U2I75_000851"
     CDS             14130..15059
                     /gene="tadA"
                     /locus_tag="U2I75_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN1696003.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15016..16065
                     /locus_tag="U2I75_000852"
     CDS             15016..16065
                     /locus_tag="U2I75_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN1696004.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16062..16340
                     /locus_tag="U2I75_000853"
     CDS             16062..16340
                     /locus_tag="U2I75_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN1696005.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16315..16785
                     /locus_tag="U2I75_000854"
     CDS             16315..16785
                     /locus_tag="U2I75_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN1696006.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16802..17104
                     /locus_tag="U2I75_000855"
     CDS             16802..17104
                     /locus_tag="U2I75_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696007.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17121..17492
                     /locus_tag="U2I75_000856"
     CDS             17121..17492
                     /locus_tag="U2I75_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN1696008.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17464..17823
                     /locus_tag="U2I75_000857"
     CDS             17464..17823
                     /locus_tag="U2I75_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696009.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17995..19002
                     /locus_tag="U2I75_000858"
     CDS             17995..19002
                     /locus_tag="U2I75_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1696010.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19027..20211
                     /locus_tag="U2I75_000859"
     CDS             19027..20211
                     /locus_tag="U2I75_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN1696011.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRGLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20648..22231)
                     /locus_tag="U2I75_000860"
     CDS             complement(20648..22231)
                     /locus_tag="U2I75_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1696012.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22809..22952)
                     /locus_tag="U2I75_000861"
     CDS             complement(22809..22952)
                     /locus_tag="U2I75_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN1696013.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23175..23654)
                     /locus_tag="U2I75_000862"
     CDS             complement(23175..23654)
                     /locus_tag="U2I75_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1696014.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23727..24011)
                     /locus_tag="U2I75_000863"
     CDS             complement(23727..24011)
                     /locus_tag="U2I75_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696015.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24089..24283)
                     /gene="ssrS"
                     /locus_tag="U2I75_000864"
     ncRNA           complement(24089..24283)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U2I75_000864"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24355..25641)
                     /locus_tag="U2I75_000865"
     CDS             complement(24355..25641)
                     /locus_tag="U2I75_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN1696016.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25779..26450
                     /locus_tag="U2I75_000866"
     CDS             25779..26450
                     /locus_tag="U2I75_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN1696017.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26416..26904
                     /locus_tag="U2I75_000867"
     CDS             26416..26904
                     /locus_tag="U2I75_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN1696018.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26917..27864
                     /gene="prmA"
                     /locus_tag="U2I75_000868"
     CDS             26917..27864
                     /gene="prmA"
                     /locus_tag="U2I75_000868"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN1696019.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27865..28629
                     /locus_tag="U2I75_000869"
     CDS             27865..28629
                     /locus_tag="U2I75_000869"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN1696020.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28862..31075
                     /locus_tag="U2I75_000870"
     CDS             28862..31075
                     /locus_tag="U2I75_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN1696021.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31086..31532
                     /locus_tag="U2I75_000871"
     CDS             31086..31532
                     /locus_tag="U2I75_000871"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN1696022.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31703..32962
                     /locus_tag="U2I75_000872"
     CDS             31703..32962
                     /locus_tag="U2I75_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN1696023.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33051..33998)
                     /locus_tag="U2I75_000873"
     CDS             complement(33051..33998)
                     /locus_tag="U2I75_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1696024.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHIPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34392..35684
                     /locus_tag="U2I75_000874"
     CDS             34392..35684
                     /locus_tag="U2I75_000874"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN1696025.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35699..37468
                     /gene="aspS"
                     /locus_tag="U2I75_000875"
     CDS             35699..37468
                     /gene="aspS"
                     /locus_tag="U2I75_000875"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN1696026.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37577..38452
                     /locus_tag="U2I75_000876"
     CDS             37577..38452
                     /locus_tag="U2I75_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1696027.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38718..40601
                     /locus_tag="U2I75_000877"
     CDS             38718..40601
                     /locus_tag="U2I75_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1696028.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40676..40960)
                     /locus_tag="U2I75_000878"
     CDS             complement(40676..40960)
                     /locus_tag="U2I75_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1696029.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40950..41684)
                     /locus_tag="U2I75_000879"
     CDS             complement(40950..41684)
                     /locus_tag="U2I75_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN1696030.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41813..42436
                     /locus_tag="U2I75_000880"
     CDS             41813..42436
                     /locus_tag="U2I75_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN1696031.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42628..43749
                     /locus_tag="U2I75_000881"
     CDS             42628..43749
                     /locus_tag="U2I75_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN1696032.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43756..46884
                     /locus_tag="U2I75_000882"
     CDS             43756..46884
                     /locus_tag="U2I75_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN1696033.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46903..50097
                     /locus_tag="U2I75_000883"
     CDS             46903..50097
                     /locus_tag="U2I75_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN1696034.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50243..50950)
                     /locus_tag="U2I75_000884"
     CDS             complement(50243..50950)
                     /locus_tag="U2I75_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1696035.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51715..52113
                     /gene="spxA"
                     /locus_tag="U2I75_000885"
     CDS             51715..52113
                     /gene="spxA"
                     /locus_tag="U2I75_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1696036.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52445..53098
                     /locus_tag="U2I75_000886"
     CDS             52445..53098
                     /locus_tag="U2I75_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN1696037.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53324..54322
                     /locus_tag="U2I75_000887"
     CDS             53324..54322
                     /locus_tag="U2I75_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN1696038.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54498..56306
                     /gene="pepF"
                     /locus_tag="U2I75_000888"
     CDS             54498..56306
                     /gene="pepF"
                     /locus_tag="U2I75_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN1696039.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18550 a         9727 c        11688 g        16416 t
ORIGIN      
        1 ggcaaaaaga tgaactagaa atagttcatc tttttttgtt taaatctttt gctaagttga
       61 actaatagag tcttttatga atatcgaagt tagtttttca cctaacttcg ttttttatag
      121 ttggttatta taaaatagta atatagctga tttggattca attcatattt atttataaat
      181 caaaaataca gagaatagta tataaatgtc ctttcctatt cttaaaatga tgtttgtact
      241 tacttaaatt aggtataatt agtatatgtt ttgtgagaaa tttagactta caataagcag
      301 tgaatttata atcaacgtat aggagacatg gatgaaatga aactaattgg tatagattta
      361 gacggtactc ttttgaattc tgaccagagc attagtcaaa aaaatgcaag aacaatgaaa
      421 catttaccgg aagactgttt tccattcatt tgttctggaa gagaagtaga agacattaat
      481 aatattctca aaaaatctgg tttaaggata ccagcagtag ggttaaatgg ttctgttgga
      541 tttgatggag aaaaaaagat atttgagtct tccttcgata aaaatgatgt aaaaaaaatt
      601 gattctgtcc tatcgaaatt tccaaccaaa atatatacaa atctcggaag ttatgaatct
      661 cagaattata aaaataaaat gaaaaaaatt tttcacgagg tagggagtga attttctatc
      721 aaagagctaa actatgagct tgagtatgaa aaatctattt gttcaatccc ttataataat
      781 attgaggaaa ttatagaaaa ggatgctatc agcatttaca aattttttat ttttattcct
      841 aatcgtcaat tgaaagctaa tatatttaac catttgaaaa agatgctaaa tgtgtctgtt
      901 actgaatcgg cacctgtaaa tattgaaatc gtacctcaaa atgtatctaa aggtgtggtg
      961 tttgatcgtt tggaatcaat ctacaattta aagaaacctt tgagaatcgc gatcggagat
     1021 agtttaaatg acttatctat gtttgaaagt gcagatatta gttttgcaat gggaaatagt
     1081 catcaagaaa tcatgaaaaa agctgattat gtcactgttt caaatgataa agacggagta
     1141 tctcaagcat ttgaaagaat tcttaccata taaactaatt aaggtagtat agaacataga
     1201 attctaaata cggaaaaatg gagcataaat tccagtacaa atagtacttc ttcagtgtca
     1261 aacgaagcga atataacata gatatttagg attgtgacaa aaatcatgtc acaatctttt
     1321 tttcgaataa acttattttt tgaagtcggc catttatcgt aatttctttg cataatacga
     1381 acaaattttc ggttattgat gaaaaaaaac attgaaaatg gtatgataaa agaaaaaaag
     1441 gaggaaattt ttaatggtga aatttatcca tgcggcagat ttacatatgg atcggtcatt
     1501 tgaaggactg acaactcttg ataaaactgt acaggaaaaa ctattgaaga cgaatctgac
     1561 cgttttatca aatatcattg aacaggcaat aataaataac gttgattttg ttttacttgc
     1621 aggagataat tttcatcaaa atcgtccttc attaaaaata cagaaacatt tttcagaaca
     1681 gatgaaacgg ttagaaaaga accatatacc tgtatttata atattcggaa atcatgattt
     1741 ctatcaaaaa gagcggtatt ggttctcctt tcctaaaaat gtccatttat ttacatccga
     1801 aacggtagag acaaaaaaaa taacgataaa aagtggagaa actgtctctc tcagtggatt
     1861 tagttatcgt caaccgtgga ttcaaaaaga caaagtgatg gaatttcctt ctagagaact
     1921 cacagattac catattgggt tgtaccacgg ggaacctggc gtctctcaaa aaggaaacta
     1981 tgctcctttc caaccctcaa agatgcaaga aaagggatac gattattggg ctttagggca
     2041 tatccatgtg ccaacggtct taaatgaaaa gcaaacaatt gtttatccag gtgcgcctca
     2101 aggtcatact aaaaaggaac aagcttctac atctattcta ctagttgaat taagtaaagg
     2161 aagttgccaa atacatccga ttaaagtagc agaggtctat tggaagacaa aggaaatatc
     2221 attgaggaaa gcaagaacga caaaagaggt cattgttcac attcgtcagc agctttcaaa
     2281 agtagaggat ggatttagtt taattgagat aaaattaaaa gattatgacc atctcaatac
     2341 agatgttttg gaacggatac aatcaggaga gctattagac tatttgttag aggaattctc
     2401 agataggata aatgatttct ttatttggcg tatttctctc atagagaata ctttttatac
     2461 gaagacccca cttgctgctt ctgctaaatt gatggaacag ttatttcaat actatcagac
     2521 acctcaagat ttccaacaaa ttttaaatga agtatatagt catcaagaag cggctagaat
     2581 attgagcgaa ctgccggaat atcaagaaga aacacttgaa aaagccaagc agctgcttaa
     2641 tcaagatttt ttatttgagg aggatcagga atgaagattt taaggatcga gattgctgct
     2701 tttggaaaat ggcgtcaaaa aagttttgac ttttattcag gcaatcagct gatctatggg
     2761 ggaaacgaag caggaaaatc aacgatttat caatttattc aagcaatatt atttggtttc
     2821 ccttcaaaag gccgtaaaaa aaaggattat acaccaaaag acggctcagc ctatggcggc
     2881 aaaatatggc ttaaacatcc tgtatatggt gaatttgctg tcgagcgcta caagcagcaa
     2941 aatcgcggaa aatcgaaggt gtggctgggg gatcaagtgg gaagtgatga gttactagaa
     3001 aaactgatcg cacctttgac gaaagagtta ttccagcagg tatttacttt ccagcaagag
     3061 cagttgatgg aactagatca tttgagagaa aaagagctac atgatgcgct gatttcttta
     3121 ggcatcacgg gcagttctca agtattccaa aagcggcaag aatattatca gaaagcacaa
     3181 caattattca agaaaaaagg ccaaaaatta ctaatcaatc aaaaattaaa tgaatggaaa
     3241 gagctgcaag aaaaaattca tcaaaaacaa gaacaggaag cttattttgg caagttgatc
     3301 caacaagaac aggaatacga gtcgcaacag cagcagttat tggcacaatt aaaagaggcg
     3361 aacaaacaat tttcattagc cagacagcaa gaacttaatt tttctttata tgaagaatgg
     3421 caatctctca aaaaaaacag cactacaggt cttcctgatg agcaacttct tttagatctg
     3481 gagtctttct ccaaacggta tcagcaaaca aatgagcgtt tggaagaatt agaagcaaca
     3541 ctggaaaagc atagcgggat ggatcagcag tcagccagat attattttta tttagagcag
     3601 gagcaggaac taaaagatct ccagcaactt caatttacag cggaacaatt aaacgaagaa
     3661 tggcaaagat tacaaattga gactagagaa acaaattcag aattgaagat tttggaacaa
     3721 aggtggcatt ggcagaaaaa tccgttaccg gaagaatttt tatatgaaga agaatggaag
     3781 catcttctgg ctgaagaaag aaggattgct gatctagaaa gccagatttc tgtgcgtctc
     3841 caaatagcta aagaacagca agagatgatt gaagcagaag tgaatgactt ggaagagacc
     3901 tatccagcat tattacaaaa tcagtcagag atagcaccag tctcttcatc taaaccgtat
     3961 atgctgatgg ctggcggagc aataatcatt gcaggtatat ttgtaccaat gcccttgaaa
     4021 gtgatccttt tactaattgg tgcaggcatg attggctatt tcttctacaa aaggcaacca
     4081 aaaattaaaa ctaaaaatga tttctctgag ataaaaggaa tgtggcaaga aaaactagga
     4141 caacttgatc tagccttagc tcaattagct gaagcagaag aaaaacaaaa gagaactgga
     4201 gaagaaaaac agattcttca gcaagaagta tatcgttttg cggatcatcg gcacttagga
     4261 aagatggata cgctcaaact catgaaagaa tatggacagg aagtcataga ttatcagaaa
     4321 ttatatggcg tatttgagcg gaaacataaa agagaacagg aaatacaaga aaaactacga
     4381 caaatcgacc aaaaatttga gtttgttaaa gaatggttac cgatacaaga taagacaatt
     4441 tcagaaaaaa tggtattgct ttcccgtttt atacaagaga tggaacagat gaaatttgcc
     4501 agaagctatc agcaaaatac tttattgaag caagaaatca atcaattgaa aaagaaacaa
     4561 aaagtatgga tacaagaata cagcgaacaa ttgactcaag caggcttatc ttatccttcg
     4621 gaagtacctg tctttttaca aaaagccaaa caagccgaac agcagatgaa aagatacaat
     4681 gaattatcgg aaatgcttac tccactattt cctgaagagc tgacaaaaga gaaattatct
     4741 gtaagactgc gccaactgaa agaaaagcaa tcccaactcc agcaagaaga aaaagaagca
     4801 agtgaaaatc ggcaacgact ccaacttcaa gtagaagagt tgcaaagaga cggcacattg
     4861 gacgaacttt atcaacaagc tagccggcaa aaagcaaaat tacaggaatt actggaggaa
     4921 tggggagcac ttgaaatcgc cgggacattc ttaggtgatc tttcaacaga gatgagtgaa
     4981 agacaattac cccagttgtt aagagcagca ggtgattatt taggtatttt aacaaaagga
     5041 aattatcaga agatattact gaaaaatgat acactgactt taacagacgg atatacagaa
     5101 tttcccatct atgagttatc tacaggtacg aaagatcagc tgattatggc gatgagattt
     5161 gcttacctct cattagaagc taaccgttcg atatgcccga tcatcattga tgatggctgg
     5221 cttcattatg atcatcagcg aaagtatcaa ttagctgaac ttttagctaa atttggagaa
     5281 aagtatcaaa taatttgttt ttcttctgat aaagaaatgg taagctatta ccatgagatg
     5341 aaccaaatcg taaaacaatt agaaggagca aataatgaaa aaaattcgtg atttagtaat
     5401 cgatgagcag tttgaacaat ttgtcctgat caaaagtgca gatgtaagaa tcgctaaaaa
     5461 cggaaaaaaa tttattgctt ttactttcca agatacttca ggtactatcg atggtaaata
     5521 ctgggatgct tcggaggagg aaatcaataa atttacagcc ggcacagtcg tttttctaaa
     5581 cggtaaaaga gaggtttatc aaggaaaccc tcaagtaaag attcttcata tgcgcttaac
     5641 aagggtagat gagccaggag agcctagttt atacatggaa agagcccctt tgaagaaaga
     5701 agaaatggaa gaagaaatca atcaattgtt gtttgagatc accaatgcgc attggaatcg
     5761 tattgttcgc tatctactta ataaatatca aaaacaattt tttgagtttc cggcagcaaa
     5821 aagaaaccat catgcatttg ccagtggatt agcttatcat actttgacaa tgcttcactt
     5881 agggaaatca atcgccgatg aatatcctga gctgaatcga gcattgttgt atgctggtat
     5941 cattttgcat gatttaggaa aggtgattga actttccggt cctatgtcga cagaatatac
     6001 gcttgcagga aatttgatcg gtcatctggt attgattgac gaagaaataa ctaaagcctg
     6061 tcttgcgttg aaaatcagcg aagctgacga agatgttctc gtattacgtc atatggtttt
     6121 agcacaccat ggcttattag aatatggctc acctgtacgt ccaagaataa tggaagcaga
     6181 aatattacat caaatcgaca atatcgatgc ttcgatgcag atgatgttga cttcgattcg
     6241 tcaaacagaa ccaggaaaat acaccgatcg gattttcggt atggacaacc gaagtttcta
     6301 tgtaccaaaa gatttatagc aaaaatttat ttttttcatt caaaacaaaa aaagcgagac
     6361 ataagtcttc gctttttttg ttttctttta ttttgatgaa tcagttgttt cttcagttga
     6421 agattctttt gttgaatcag ttgattttgt gtcagaactc tttgttgaag ctgtagtttc
     6481 acttgagtct ttcgtacttg atgaagttgt tgtgaatgca ctcaacacat tttcaaaggc
     6541 atcatctttg atttttacat ttgcatcttt taattcttca ccgattactt tagttgtgaa
     6601 tgcattgtca cttagtttag tttcagtagc gatatctttt agttgatctt tgtatttatc
     6661 catgtcattg cctttatttt ggtttttcac cattttgaca acgtagtatt ctgtcgcata
     6721 agaagtagga tttgttgtag taataacatc ggagatctca ccgtctttca atttgaaagc
     6781 tgcttctttt acttctgcag gaatagttgt tgtcgttgag tcgaatttaa ctttaccgcc
     6841 atcttctttt gtctctgtat cagttgattt gtctttggct aatttagaga aatcatctcc
     6901 atcatccgct gattttttca catcttttgc ttcatcttca cttgataatt tgatgatttg
     6961 tgcttctact tctggatgga aagatttcca agcagttttc aaatcgtcat ctgtaatatc
     7021 tacgtgagct ttcaagccag cttcaaaagc taaattgtta cgaatatatt ctttataagt
     7081 atctttcgta tatccagcag cttctaattg tgattcaaaa gtatcaccta atgatttcgc
     7141 ttgtttgtcg tattctgcat ccacttgctt atcggttact ttatcgccat atttgttatt
     7201 gaatacttta taaatgatca tccgttgtac taaggattgg ttcgatgatt caagttttgc
     7261 ttcatcatag aaatcagata cagtgatcgt tccgcctttc atcgttgcga tatctttatt
     7321 agtatcgcct gaacaagctg ctagagttaa aacagccaac gcactagtag ctgccaaaat
     7381 aattgatttt tttttcatta aaatttgcac tcctgtaatt agttattatt tttatatatt
     7441 aactatacca tatcccattt cctttaaaaa cgttgtcgtg gtagtttcaa acaaaattca
     7501 aaatttcatc acattcctag gaaatacgca aacttttctc acttatatag ttaaatctgt
     7561 tgagaaaata atcattatta tatttgttca ggatactctg gcagatcagc ttttagtttt
     7621 tctacttgtt cattgatttg tgtgatacgt ggttcggctt ggaatttaaa atcttcgata
     7681 tcttttttga ttgcttcttg gaaagcagga atcacagatt cagctgtttc ttttgtttcg
     7741 atcaatgcgc ttttaaaatg attcaggctg ttatttaaat catttgtcag agttgttgct
     7801 tcttccaatt cttcgatcag tttctttctt gtttcattcc cgctcctggg cgccaataac
     7861 agacctccga cagtgccagc agcagctcca aataacagtc ctttgaaaaa gtttttaatc
     7921 atcgcaggac acctgcttta cgattgtttc ttgaatagct tttagttctt ccgatccata
     7981 actatcttga tgattgacaa agtgaataga aaaatcatcc tctttgctgt agcgagggac
     8041 taaatggata tgtgaatgaa aaacagattg ataggctagt tcacggttgt tattcaagat
     8101 attaagacct ttcatttctg gaaaagcttt ttccaatgct tgagcaatct taggcacacg
     8161 cgaaaaaacc tctcctgcaa gttcgggatc atattcaaaa atatctgcta catgacgttt
     8221 aggcacaact aatgtgtgtc ctttcgttgc ttgagagatg tctaaaaaag catagacctt
     8281 gtcgtcttca tatattttat agcttgggat ttcttgattg atgattttgc aaaaaataca
     8341 gttttccata taaaacatcc tttctataaa gaataaatac ttcagcttaa ctttaccata
     8401 agttaaagct attgtaagga aaaaaacaaa ataccgtcat tttcttcgtg gtataataaa
     8461 taaaaataaa tggcggagga aaaagaatga gtttaattat cgatcatgtc acaggggggt
     8521 acggacatct ccctgtgtta aaagatatca gtttcgatgt acagcctgga gagatagtag
     8581 ggttgattgg actgaacgga gcagggaaaa gtacgaccat caagcacatt atcggtctgc
     8641 tggaagcttc aaaaggaacg atagaaatca atcatctatc cttaaagaaa aatacggaag
     8701 aatatcgcaa acaaattggg tatattccag agactccttc tttatatgaa gaattgacat
     8761 tgagagaaca tatcgaagtc actgcgatgg cctacgatat acctatggat atagcaatga
     8821 aaagagcaga aaagttgttg gagacttttc ggttatctaa tcgtcttgac tggttcccag
     8881 ctaatttttc aaagggaatg aaacagaaag tgatgatctt atgcgccttt ttgatcgagc
     8941 cttctttata tatcatcgac gaaccatttt taggcttgga tccactagct atcaatgcat
     9001 tgcttgaatt gatggtagaa atgaaaaaag aaggagccgg tattttgatg tctacgcata
     9061 ttttagctac tgcagaaaaa tattgtgatc gttttgtggt cttacataac ggagagatac
     9121 gagcagacgg ttctcttgag atgttgagaa aagaatttca gctgccggat tcttctttgg
     9181 acgaaatcta tattgcttta acaaaggagc aggcacatga gtagtttttt tcaaaaacgt
     9241 ttagcaagac atcaaaagaa aatgatgaaa tatatgcgtt atgtattgaa cgatcatttt
     9301 gttttagttt gtcttttctt gattggcggt gtgggattat attattctaa ttggttgaaa
     9361 accctgcagc caccgtttgc aattggaggg ctcataatct ctgtattttg gatgctctgt
     9421 ttattcgtgg gtaagatggc tacttttgca gaggcagcag atattgtttt tttacttcct
     9481 aaagaaaaag aaatgcaaac ctatttggaa cgagcttttc gctattcttg catttttccg
     9541 ttgatctttt tgatattggc atgcggattg gccatgccat tagtggtggt tagtacggag
     9601 caatcctttt cgatgttttt cgtctatctg atcatattat ggagtttgaa attcagtcat
     9661 ctgcaaatca agagagcaga attgtttcga ctagatcaac aagttattcg ttattggaaa
     9721 ttaggctggt ttttttcttc tctgatcttt ttgtttctat cattttattg gctaatatgg
     9781 gcaggaatag caggtgcatt agtacaggca agtctatatt actggttttt gtggaagcaa
     9841 caagaaaaag gattggattg ggagaaaatg gtgcatgtag aagaacatag gttgcatcag
     9901 atctatcaat ttatcaattt attcacagat gttcctgaaa ttacggcaaa gatcaaaaga
     9961 agacgctatt tggatcctat attgactcat attaaaagag aatcaaaaaa cacttatctt
    10021 tacttgtttg caagaagatt ggcaagaggt tctgatttta gtggtcttta tttccgatta
    10081 gtaattatag gtgggttatt gattgccgga gtagtcgact tttattttag tctgattatc
    10141 ggtttcttat ttttatattt gatcggtttc caactgttac ctttagcgaa ccagttccgc
    10201 tatatggttc tcgttcagct ttatccaatc cataagaatc agaagaagca ggcgattcag
    10261 tatctactca catgggtatt aattatcgcg tcggttattt ttggtttgat tgctgcagtc
    10321 gtattagatg ggcagcagcg atgggttccg ctagcggctt atttgattgt tactatgctc
    10381 tttgttaaac tatatgtacc aactcgtttg aagaagatgt cagactgaaa acaaagctgt
    10441 gtagggtaaa aatgagaatg aaaaatagag cgttgacagt aagaatgctg ttcgttctat
    10501 tttttttgag aaaagctggt tttttgaatg gcaattaaat gaaaaagaac gtatatgtgc
    10561 ttgacgtgta gtgttcaggc gactagaata ataagtactg caaaacaaag agtaagagga
    10621 cgggagaatg catgatggag tttcagttag accaagagtg gcggttacgt cccatcaaag
    10681 gcgacacagg aaaaacatac ataggcatgc gtgatgagga caaagtcttt attaaacgaa
    10741 acacgactcc gatgttggct gctctttcaa gggaaggaat tacaccaaaa cttgtttgga
    10801 caaaacgaac ggggaatggc gatactttga cagcacaaga gtggctggaa ggtcgcttgc
    10861 tgactcctaa tgagattggt caaagaaacg acgtgattga tgttttgtac catttacatc
    10921 attcaaattc tcttaaaacc atgctgaccc gtatcggtgg tcgtgtcatg acgccggaga
    10981 agatgctgca aaaatatgaa aaagaattac cggaagaact ccagaaaaat caatttctgc
    11041 aaattgtcca tcgttttctt cgtgagcaga tcccgtcaat agataaggaa aacttatcag
    11101 tagtacacgg agatgtaaac catcgtaatt ggttagtatg taaaaattat ttatatctag
    11161 tcgattggga ttcaatcatg tttgcagacc ctgcggtgga tattgcaacg attttaggaa
    11221 attatgtgcc actgtctagc tggaatcagt ggctgatcag ttatggcata cgcccgactg
    11281 acgaaatgct tgaaaaattt cattggtatg cggccatgaa tttgctccaa gaaatcacca
    11341 gatattgcaa acgtgaagat catcgacgga tgaacgaaga aattttacaa ttaaaacgaa
    11401 ttttcagtgg ctgaggtttg gcccaaactc tgcggagatt tggccagacc tttttaatta
    11461 gaggaaagga atggagataa ataatgcgtg tccgaaatcg tccgggagca gcggaaatgt
    11521 tagcggctca cccgaatttt gtaattagtg atccaacatt gtggaaggga aaatggaatg
    11581 aattgtttga aaacgatcat ccaatccaca ttgagattgg tatggggaaa ggccagttca
    11641 tcactggaat ggcaaaagcc catcctgaaa tcaattatat tggagtagaa atgcaggtca
    11701 gtgttgtttc gattgcacta gacaagttga tcgaacaacc gcttcctaac ctgaaacttc
    11761 tacatgtgga tggatcagcg ttgacagagt atttcgctga cagcgaagta gatcaaatct
    11821 atctgaattt ttccgatcca tggccaaaaa aacgtcatga aaaacggcga ttgacatata
    11881 agacttttct tgctgtagat gaacagattc ttcgtccaaa tggagaaatc catttcaaaa
    11941 cagataatca aggattgttt gagtattctc tggcaagttt ttctcaatat ggcatgatcc
    12001 tcaaacaggt atggttagat ttacatcaaa gtcaatttga aggcaatatt atgacggagt
    12061 atgaagaaaa attttcttca aaaggtcaac gtatttatcg agtagaagct caatttcaag
    12121 ataagaccaa aaagaaaagt ctgtgaaact aactttctgc tttcataaaa acattaatat
    12181 ccataaataa atcccagaaa atgaacatta cagatgtaat gtattttctg ggatttattt
    12241 gatttaaaat gtatttattt catcggtttt tccacaaggt ggaatcaatt ggaagcgaga
    12301 aaatgaaaat aatcctttgt cggtgatctt caattcgggt atgacagata atgttaagaa
    12361 agaaagtgtc aaaaaaggat caaaggtttt agctgctcct agttgatgtg ctttagttat
    12421 taattcattc aactgttcat agacgatcga aaaaggttcc tgtgtcatga ttccgcctat
    12481 aggaagagaa acacaagcta tttcctgctg tttgttgaca acggtcatcc cgccaccttt
    12541 ttgaatcaaa agattagcag catacagcat atcctcgtcg tttgtcccaa cgacaactag
    12601 attatgcgag tcatgggcaa tcgttgtcgc tatcgctcct tgtttcagct gaaatccttt
    12661 cactatgcct aatccaatgt ttcccgtttg atggtgacgc tcgatcaccg caatctttaa
    12721 ttggtctttt tcgattgaag gaatgaatcg ttcgccttgt cggtccactt tttctaccaa
    12781 atctttagtc actagactat tcgggataat ttcgatcaca tgggcttgtg aagaagaaag
    12841 aagcagctct aacgaatgtg gatttaatgg ttgagcgttt agttttggca gacaacacgt
    12901 atatgaattt gaatgatcag caaataaatg gtcttggagt tgcccagatt gaacaactaa
    12961 tgttccctta tagaaaactt catgaattgg cagcttggta agatcgtctg ttaagaagaa
    13021 atcagcttga tacccagggg caatcgcacc tagtgttttc aaatcaaaac attctgctgc
    13081 attcaatgtt gccatctgaa tggcttgtaa tggatcgatt ccaagtgcaa tagcttgacg
    13141 gacgatatga tcgattgatc cttcttttac taaatcattg atcagtttat catcggtgac
    13201 aaagaagcat cttcttgaat tttcaggtgt gatcgccggt agtagcgatg ccagatcttt
    13261 tgcaacggtc ccttctcgaa ccattaaata cataccaaga tccaaacgtt ctttagcttc
    13321 ttttgcagtt gtagcttcat gatccgtccg aatacctgct gcaggataaa catttaactc
    13381 ttccatgccg ataccagctg catgcccgtc aatttttttc ttatgctggt gcatcaattg
    13441 gatcttttct aggatgtctg tttcattatt tgctactgct tgatagttca tgacttcagc
    13501 taagcctaaa acttcgggtt tttgtaaaaa aggggctaat ttttttgcat ttaaatttgc
    13561 accattggtc tcaaaagggg ttacaggtac acaagaaggt agcataaaaa agatattcat
    13621 gagtgtttgt cttgcatctt ctatcataaa ctcaagacct gtactcccag caatgtttcc
    13681 tatttcatgg ggatctgtca ctacagtagt aactccatga agcaaggata cttttgaaaa
    13741 ttcgcttggt gataaaagtg agctttcgat atgcacatgg ccatcgatga atcctggcac
    13801 tagatattgc ccattagcat tatgtacgct ttttccttcg tattcaccga ttcctacgat
    13861 cattccttcg cagattgcta catctttttc catgatttct ttcgtaaaga cgttgacgat
    13921 cttagcatgc ttgatcacta ggtcagcagg ctttttttta cttgctactt cgatatattt
    13981 ccttaatgtt gtttccaatg gagtgcctcc caagataaat ttttataagt ataacataaa
    14041 ttcggttttt gccaatattt ttttgtaaat tcattttatt tgaaacaaaa agatcaaaaa
    14101 gacgtatgtt ttttgtggag gggagaataa tggaaataaa acgattatcg gatcaaatga
    14161 taagtgaaag ttttgccaaa catgcccaag acgtatatat attgccagct tcagaagtat
    14221 tttacatcta ttttcgctat ggaaaaaaac gaacattctg gaagaaagtc tcttcggcag
    14281 atggacagca actgatctca agatttaagt atttgggaca gatggatatc ggagaaaaaa
    14341 gaaaagctca gttaggtgca tttacttatt ctttagaaaa acaggaagtt cgtctgcggt
    14401 tgtcttctgt aggagattac cttcaacgag agagtttagt gattcggcta ttatacggta
    14461 cttttgaaac accccatcgt tgctttagtt ctcgagattt tgcaactatt ttacagaaaa
    14521 ccgtcagacg tggattatac cttttcagcg gaccagtcgg atcaggcaag acttctctaa
    14581 tgtatcggtt ggctttggaa gaaaaattgc aagtgattac tatcgaagat cctgtagaga
    14641 tagaagaacc gttttttctg cagctacaga tcaatccgaa aatagcacag acttatgata
    14701 cacttttgag actcgcatta cggcatcgtc cagacttgct aatcattggc gagataagag
    14761 atgaatcaac tgcacatgct gcggtaagag cagcactaac agggcaccgg gtttttgcaa
    14821 ccgtacatgc acgcaatctt gaaggtacat tggtcagaat ccatgaactg acaaagaaaa
    14881 aagaagagct atctgaatgt ttagctggtg tcatttatca agaacttttg gaagatgcca
    14941 atcaccatcc ttctgtttta tggagttatc aatttatgga ggaagaatca gtaaaaaaat
    15001 catgggagga aagttatgaa gaagctcagg aaaatatttg gcagtcagaa ctattctaaa
    15061 ggattaacaa gaagccagca aaaccagttt gcacaaacca tgggagaact gcttttatct
    15121 ggttttagtc tgcaagaatc cctttattta ttactcacga tccgttcgtt tccacctgtt
    15181 attttggcaa atatccaaca tcagctaaag gaagggcaag cgtttagtga ggtactttcg
    15241 caaataggat atggagcaga gcaactggta cagatcgagt tagctgaacg tcatgggaat
    15301 ctgccgacaa ctttgttgga aattgccaaa cagtttcggc ttgttgaagg atttcgcaat
    15361 gatttgaaaa agctgcttgc atatccgatg ctattgctca cctttttgct aggtatcttg
    15421 tttagtatgc ggttgttcgt tttgcctcag cttattggtt caggaatggt catgcaagag
    15481 cattggggta ttcagctgat acaagtcttt cattggtatg ttctactatt catcctagcg
    15541 gttactctat tggctttact agtaaggtat cgtctgatga agttgtctcc tgttgatcag
    15601 agtgaatgga taagcagaca attattgatt ggaccgatat attccttgta tcaatcggct
    15661 tatttgtcac tagagattgg aaagcttttt catgaaggtt tcgaattaag gcaaatcata
    15721 gtctgcttaa aggaaacgaa acaagaaagt ctgattcagc atcttgcctc taagttgatt
    15781 caaggattgg aggcaggtat gtcattggct gagcagttcc agaattatcg ttttctatca
    15841 gaagaattca gtaaaattgt tttacaggga gaggcaaagg gaagtttagg taaagaactg
    15901 attttttaca gcgaatggac aagaaaacag cttttcaaac gtcttcatca ctggatgcat
    15961 tttattcagc caatcatttt tttgggcgta gcggctttga ttttattagt ttatgccgct
    16021 gttttattac ctgtttacgg aagcatagag gaggttttgc catgaaaaag agaaaaatat
    16081 cttattatag cggttttaca ttgatcgaaa tgctaatcgt cttactgatc atcagtgtat
    16141 tagtgttgtt gttcgtccct aatttgtcgc gttataggaa tcatgtagac caagaaagtc
    16201 gtgaagcgat catccagttg gtagacacac aaaaagagct ctatgcgctt caaaataatg
    16261 gaagagttcc tactgtggag gaattgttga atgaaggcta tatcaaacga gaacatgcag
    16321 aaatctatca gcggccttga aggtaaggtt ttagcatcag gatatacgtt gtttgaatca
    16381 ctcttactga ttttattact tacaacgatc ctttcttttc cagttctcgc attttctgca
    16441 tggaagaatg aattggcagt ctatcaattt ttcggccaat ttgaaaaacg aatctattct
    16501 acgcaaaaaa tcgctattgt gaatcagatc caaacaggct tttattggaa tagtgaagag
    16561 aatcaaatca tttttcatgt tccgaatcca gataaaataa attgggaagt attagatatt
    16621 cctgatgaga ttaccttgaa acgccatgct tctattactt ttgctgctag aaccgggaat
    16681 gaaagttcac taaaagctta tcaattttat tgggaagaaa aagagcggac gattacgtat
    16741 caattccaga tagggagtgg tcgctataca aaaagaattg aataaaacag gatttgtttt
    16801 gttggagtcc cttacagcgc ttatgatcgc ttctgtctgt gtctacttag tgagctttgg
    16861 ccagctgttt ttgcttcagg cagaaaagaa aagtggggag gaactgctat ttattcgaat
    16921 gatttatgaa gatatcaaga cgcagcggat gtatcacaaa agaccagagc gaagggaaga
    16981 aatgatccca tacgatatcc aaattgtcga taataatgga gtaatcgaaa aagtgtttgt
    17041 caatatggag aaagaagcga tcagtattga aaaaacaagt aattccagca tttacgctga
    17101 ttgaatgtct aattggactg atgatattga gcagtctatt gctcacattc aatcttttga
    17161 ttcagcaaac catccaagta agtgaatttt tgcagcgtaa agatcaaaaa gagtggctaa
    17221 tatttttagc acaattagaa gaagaactca agagcagtca caatgtctct gtgaaaaatc
    17281 agcaactgca ctacacgatc gaggataaac agtatatcat cgaaagatat caggcaatga
    17341 tacgtaaaag aagaacttcc ggcggacatc aacctatgtt aacaagtatc actgagcttc
    17401 aattattgga aaaagaacac acaatttctt tttatgtaca ctttgaaaat ggagaagatg
    17461 gatatgccat atggacaaaa aacgatcagt agaaaatgtt ataagggctc tattttattt
    17521 tctgtcttgc ttttatttct tttatgtacg tttttattga tggcagcatt agaaagctac
    17581 cgtttgtcaa tggatcttaa tatacgaatc aaaaattatt atatagcaaa aataatgaag
    17641 cagatggctt tatcggaaca gtctacgctt gccgaaagtt cggaaggagt tttttactac
    17701 acaactggat ctgtaacata tcagtgggta caacagtccc tattcttaga ggtggaagtc
    17761 tcaccgttta tctttcggtt cacagaagaa gtaaaaaatg atacagatac tagtacagaa
    17821 tgatggaatg ttcgtgaaaa cgccctcttt tctgttataa cactgttttt aataaaaaaa
    17881 tgtttgccgt ttcacaaata aaaaacagtg aaaacgattg aaattttgtc aattgttcga
    17941 tagaattaaa caggtgaaaa aattgttata atatatctag aaatgaggtg caccatgtta
    18001 ccagaaaaaa tggaacaagg gtttaatcaa aacctggaag ctattcagct tttacagaat
    18061 gcattgggaa cttccttttt agaagcctat gtcgaaaatg cagaaaattt aatagatgat
    18121 tatcaagtac gtgtggtgga tggagtgccg acaaaagaga caacgcaacg gatcacagct
    18181 ctttatgaag agttgaagaa actttctttc gagccagaag aatggcgcag actgtctcaa
    18241 ttacttttat tgaagggcag ccagacagaa catttgcagg cgaaccatca actcactcct
    18301 gatagtatcg gttttttatt tgtttttctt attgaacaac tatacacaaa taaaaaagca
    18361 ccggttaaga ttctggatat tgcggcagga atgggcaact tgttattaac agttctttta
    18421 aacctatcga atgcggggta ccaaacagaa ggaattggtg tggatattga tgatacattg
    18481 ttggctgtcg cagcttctac aagtgatttg acacaagcaa atgttcaata ctttcatcaa
    18541 gacgggttgc aggaattatt aatagatcct gtcgattttg caatcagcga tttgccaatc
    18601 ggttattatc cgaatgacga aaaagcaaaa gagtttttaa caagtaccga agaaggccat
    18661 agttatgcac atcatttatt gctagagcaa tcaatgaaat atgtgaagcc ggatggtttt
    18721 ggtttgtttt tgatgccgag tggattttta gaaacggatc aaagtgaaga aatcaaaaaa
    18781 tggtttaaag aagaaggtta tcttcaagga atgattcaat tgccagatga attgttccgt
    18841 aataaacaat cgcaaaaaag tattttgatc ttacagaaaa aaggaccaca agcacaacaa
    18901 gtaaaagaag tcttgcttgt gaaacttgct tcattaaaag aaccagagaa ggtgacggaa
    18961 tttttcaacg agtttaaaaa ctggaaatca tctgctctgt aatcattcaa cgagaaggag
    19021 agtcatatgt ctaaaacaat tgcaatcaat gctgggagtt caagtttgaa atggcaatta
    19081 tatcaaatgc cagaagaaac cgtagttgcg aaagggatcg tcgaacgtat cggtttacaa
    19141 gattctattt ttaccatcaa gtacggcgat aatcaaaaat tcgaacaaat catggatatc
    19201 gaaaaccacg aagtagcagt aaaaatgctg ttagatcagt tgattgaact gcaaatttta
    19261 agctcttatg atgaaatcac cggagtaggg caccgtgttg ttcacggagg agaaacttat
    19321 ggggattcag tagtgatcga tgagaaagta atggaccgca ttgctgaatt agcagaattt
    19381 gctcctttgc ataatcctgc aaacttaatg gggatcaaag cgtttaaaaa gattttacct
    19441 gacattttga gtgtggctgt attcgataca tctttccact caacaatgcc aaagcaaaat
    19501 tatttatata gcttaccggt gtcttattat gaagatttca aagtaagaaa atatggcttc
    19561 catggcacaa gtcatcgtta tgtgtcggaa cgtgcggcaa aattattgaa caaaccgatc
    19621 gaagaactaa aaatcatcac ttgccactta ggaaatggtg tatcgatcac agcagtcgat
    19681 ggaggtaaat ccatggatac ttctatggga ttcacaccat tagctggggt gacaatgggt
    19741 acacgttcag gtgatatcga tccagctgtt ctcccatatt taatggaaaa attagagatt
    19801 gatattgatg aaatgatcaa tgttttgaac aagaaatctg gtctgctagg cctaacagga
    19861 ttgtctagtg atatgcgtgg tcttgaaaag aactatgaaa aagaacatat tcgtttagct
    19921 tatgacattt tcgtggaccg catccgaaaa tacatcggcg gttatgtgac aacgatgaat
    19981 ggtgttgatg caattgtctt tactgctggt atcggcgaga atgacggaca tgtacgtagt
    20041 gaaatcatca aaggtatgtc ttggtttggt tgtgaaatcg atccagcgtt gaatgaattg
    20101 cgtggtgaag aagtagatat ctctacaaaa gaatcaaaag taagagtatt agtgatccca
    20161 acagatgagg aactaatgat tgcccgtgat gtggagcgtc tgcgcggcta agccaaagca
    20221 ttcgttagga taaaagatga aacgcatgca agagggaatt cttgcatgtg ttttttttgc
    20281 ggagaagacc gcaaaatggt aatttaactt caatttccct tcttttttac aggatcttct
    20341 tagggaattt tgcttggaaa agtggtcaaa agggaaaaga ctggcatttt atttattgat
    20401 tttcaggaaa atcccaatat tttattttca ttcatcttta taagggacat tacatatgta
    20461 aaaagaaaaa acatttttag atcagcctcg tgggctgtcg actttattct tcgctttatg
    20521 tttagctaga aaaccgatcc gaaacattca ttgatcgaat aataaaaact tgattttcca
    20581 cctgtacttt gcctgaatcg tgaaagcaca ggtatgaaaa tcaagttttt tgttgcgaaa
    20641 aaacgtccta gtcaatcagt tggccgagcg gctggcggct gatctgctcc tgatcaataa
    20701 cgagtgcttc ttggaagtgt tgctgcaaaa agtagcggtc atggctgact gctaggagcg
    20761 ttccttgata gtcttgcagc atttcttcaa tttcttctcg agcatagctg tcgaggtgat
    20821 tggttggttc atccaaaatc aggacgttga ttttttctaa aaagagtttc agcaacgcca
    20881 aacggatttt ttcaccgccg gataaatctt tgatccgttt acccacatcg ctttgataaa
    20941 agccactttt ggctaattgc cgtcgagcga cttcttcgtt tccagtcaat gtgatggcgt
    21001 aagccagcaa acggctatcg ggatcggcaa actgcagctg ctgcggcaaa taaccgattt
    21061 gcaagctact acctaatttg atggtgccac cgtaaggctt ttcctcaccg agtaataatt
    21121 tgagtaaggt tgattttcct gcaccattgt cgccgatgat ggctaaatga tctccacgat
    21181 agactgcaaa actgctgtct ttgaatagta cttgatcagc gtactgctgg tgcaagtttt
    21241 gaatgatgaa aacttctttg ccagattttc caccattaga caagctttgc agccgatttt
    21301 tcgggggagg tggcggtttg acgagtgtca gtttcgccag gcggcgttcc agttctttgg
    21361 cttttttgaa gaaggactca ttgtctcctt catgtgccca ctgacgaaaa cgacgaatca
    21421 tgactttcaa ccgctggact tctttttgct gcagttcgta attttttgtc agctcagcca
    21481 agcgagcttc tttcaagact cgaaaacggc tgtagtttcc gggatactca atcaatgcac
    21541 cttcttccag ttcccatgtt tttgtggtga cttgatcgag aaattgccga tcatgagaaa
    21601 cgaccagaac cgattttttt gtattctgta aatagttttc caaccagcga atactgttaa
    21661 gatctaaatg attggtcggt tcatcgagta aaagtaaatc ggcatcggct gctaaaattt
    21721 tggctaaagc gactttaact cgttcgcctc cgctaagttc tgttaaccgt gcttcctgac
    21781 ggtgtggaat ccctaagcct tgcaagatcg ctgcaatccg gtcttctaat acgtaaccgc
    21841 ctagttcttc aaagcgatgc tgcagctcac cgtaacgggc cagacacttc tctaagtttg
    21901 tatctggttc tgtcatcaat gcttcgtagc gactcatttg ctgtttgagg tcctgtatct
    21961 cagcggcaga atggagcaag tagtggttga cggtcgcttc ggaaactgta agttcttgtt
    22021 cgaccgtccc gatctttaag ccttttttgc gactgataac accgctgtcg acaccttctc
    22081 tgccggtcaa gatttgtaaa aaggttgatt tgccagttcc gttgataccg actaaggcaa
    22141 ttttttcacc agcttcaatt tgacaggata aatcctcaaa taatggattc ccgccgattg
    22201 tttttttaat agaaattgct tgtatgatca tgatctctct ccttgagacc actcaaaaag
    22261 gccatggtta tccatggcct gtgttattta aactcagatt atcatgagaa aaataccttg
    22321 agatggccat tgattttacg tgaattcaac tgttttggac aaacttgtcc cgtcaacagc
    22381 aactgtaaaa ccaattgcat ggctaaatgc gatatggtat cgttggtaat tttcattact
    22441 tgttatttta gtcatgcgca tcatagtatt ttccctcctt cgtgtagttg tcttttccag
    22501 tgtacctgaa agtgtcagaa agttcaacta gcgctgacat ttacttaata tttatcgatg
    22561 tgtaattctg tcagagagct ttttttatct gactgagggt aatgtctcgt ggaccacatc
    22621 attgtattct gagctaaacc agcgttcagg cgaaatcata tggtgtttga taaaaaactt
    22681 tgtctacatg acttcctttt gccatttgct taataccgtt gcattaaaca gctcttctct
    22741 ttggaaatct caccaacatc gcgttcagga gtatcgttgt ggttgcaatg gcaagcggta
    22801 gcctttcatc aattcttgaa tgatttaccg aatgtagcac ttgccttctc gcggtacccg
    22861 aacccgaaat aaaattttag tttggcgata aaatcggtat ccgccatcga tacttcatat
    22921 tttttcgtat cagattcatc ggaaacttcc acattgacga accaataagc tttggcatgc
    22981 ttaggtcgtt ttaaaccaca ccagggcgac taatcgccct ggtgttcact tttttttatt
    23041 ttacttgaac tcattgactg ttttcccttt attttaaaca ttataaagac cgatgagtgc
    23101 ttgccgcgtc ttttgttttt atcacggttt tttgtgcaaa aaaacctgcc gatagttggc
    23161 aggtcgtcta cgtgttaagc tttggtgtgg tctttagctg gcaattggtt ttctaaatca
    23221 gttcggacaa caagcacatc gcaagctgcg tttctaataa catattcaga aacagaacca
    23281 ataaataaac gttctacagc attcaatcct gtggctccta acataatcaa atctacttga
    23341 ttatcttctg ggatttctct ggcaataatt tgttttggtg agccatattc tacgacagag
    23401 gttacattat tcaagccagc tttcttcgca ttgctttcat aatctgccaa tgtttgcttt
    23461 gccatttcag tagcttgttc tgcaagcatt ccatcaaagg atgaaacggt ttggaaagaa
    23521 cgtgtgtcaa tgacatgtgc taatagaagt tctccgttat ttcggatagc tacattgacc
    23581 gcctttttaa aagctaattc agcttcatca gaaccatcta cggcaaccat gatttttttg
    23641 tattgttgta acataagcat tcactcctca agtcaatcgt attgtactct tttattgtag
    23701 aacactttag ggaaaaagag aattatttac tgctttggtt cagcaatttt ttttgcaaat
    23761 cggatactga aggtggcaga aacaataatt acgatcagta aaaagaccaa ggcaataaag
    23821 cgttgattga aaaaagctgt taaaataccc attacgccta aaacagcata tgcagctccg
    23881 taaaattgta ggaatccttg atttttatca tttttgttga tcaatacaaa aaagatatcc
    23941 tgtttattaa ataaataata gctaacgaat aataatacta aagtggcgat aatcattaaa
    24001 atcctaatca tgaacaaatc ctccaaaata agcaacaaat agaaaaagca atttgccaag
    24061 agcaaattgc tttattcttt aaaagatggt aatctccgtt aatgccgttg tttgtcctgt
    24121 agtattgatc ccgcgaattc aagcaggtgg gtcccatggt cgtagcttcg aactaccaaa
    24181 tgataaggca tgttaccagc agtgacacaa gacgggtccc aagatatcaa taaaaatgtt
    24241 cggtcaacac acaaagagga caattcgaac atcacagatt tcccacctct atagtagcat
    24301 atgaatttaa aaagagcaat gagagagctc gcagaaacag aaccgttttc ttgtctagtt
    24361 ttttggagga tgctttttcc actcttttat acggtaatac tgttgaccca gagcctgttc
    24421 atatttccct gtatcttttg gctgataata ctgagcattc ttcagtttat ctggtaaata
    24481 ttgctgattg acccaagcct gatcaaaatg atgaggatac tgataaccca cgccacggtt
    24541 gagtgatttc gcacctttgt aatggctatc tctcaaatga tcaggaacat ctccagcttt
    24601 gccctctcga atatcagcaa gtgctgcatc taatgccatg taggcagaat tggattttgg
    24661 cgacaagcaa agatcgacga cgacgtctgc tagtgggata cgtgcctcag gcaatcccaa
    24721 tttttcagca gctaatacag cgttcactgt acgagccgca gcagcaggat tacctaaacc
    24781 tatatcttca taaccgataa ccatcagacg ccggcaaata cttgccagat cacctgcctc
    24841 aactaaacgg gcgagataat gaagtgcagc atccacatca cttccacgaa tcgatttttg
    24901 aaaagccgaa ataacgtcat aatgagcatc tccatttttg tcatgggtaa gagcttttct
    24961 ttggacacat tcttcaatga tcgacagtgt aagatgaatc cttccttctt tatctgaagg
    25021 agtcgatagt gtagccagtt ccagcccatt caatgcactc cgcaaatcgc cgttcgttgc
    25081 acgcgaaagg tgaagaagag catcttcgtc taactggatt gcttgttgtc caaggccgcg
    25141 ttctttatcc cttaaagcat gttcgactgc caactggatg tcttgttctg ttaatggttt
    25201 gacttcaaag atctgtgtac ggctgcggat cgctggattg atggtaatat aaggattctc
    25261 agtagtcgct ccgatcaaga tgattcggcc actttctaaa tgaggaagca gaaaatcctg
    25321 ttttgtttta tctaatcggt gtacttcatc caataagagg atcactgtac cactcatttt
    25381 tgcttcttcc gctacaactt gtagatcctt ttttgaatct gtagcggcgt ttagcatgcg
    25441 aaatgcataa ttagtagagc cggcgatcgc acttgcgata ctcgtttttc cagtaccagg
    25501 aggtccatat agaatcatgg aagagagcat gcgagcatcg accatgcggc gtatgatttt
    25561 tccttccccg actagatgct gctgaccaac gacttcatcg atcgttcgag gtctcatgcg
    25621 ataagccaat ggctgttgca taggattttc ctccttcttg tttttacgtc ccttaattat
    25681 atcatattta gcaagatgcc tagaaagaac agccttctgt tgttttgttt tcatagctta
    25741 tccaactaat attgtgtatg ataaagtagg tgagaaaaat gacggataaa caaaagatag
    25801 atgaattttt ttggaataaa acgacgccag aagtggcacg ggatttattg gggatgtatt
    25861 tggagcatga cacaccagaa ggaagattag ctggatatat tgtagacgcg gaagcatacc
    25921 taggtccaga agacgaggcc gcccatagct atggtttaag aagaacaccg cgtgtgcggg
    25981 caatgtatga aaaaccaggt acgatctacc tttacacgat gcatactcat cggattttga
    26041 acatcattac tcagccagaa gggattcctc aaggagtaat gatacgtgca atcgaacccg
    26101 ccgcaatgat tgatcaaatg agtaaaaatc gtggcggaaa aactggtcca gatatcagca
    26161 acggtccggg aaaattagtg gaagcattag caattcctca agaactttat ggtcagtcta
    26221 tagcagatag ttcgttacgc ttagtttttg agaaaaaaaa gacaccaaaa aagatcaatg
    26281 ctttgcctag aatcggtatc ccaaacaaag gtgtatggac agaaaagccg ttgcgttttg
    26341 ttgtgtcggg aaatccttat atctctttac aaagaaaaaa ccaaatcgag aaagattggg
    26401 gttggaggaa agaaaatgaa aaagaaggat ctattaacat atttagatga aataattgaa
    26461 aaacaagtga cagactatga tgtagcttta gattgggata caaaaaatca tacgatcgag
    26521 atcgttgttc gcttgtttgc agaaaataaa gcacaatttg agattgatga tgcagaaggg
    26581 atcgtttcgc aagaaccaat tatcgaattt gaagacggcg tacttctttt taatcctcag
    26641 aaatctgttt ttgatgaacg caattatcta gcagtaatcc cttatgaagg aaaaaaaggg
    26701 ttggcaaaat cagtagctga ttctttagtg gaatatctaa atgaagtatt agctcaaggg
    26761 caaagcgatt tgcttgattt tttggatgaa gacgatgacg aagctgtatt cgagcttcac
    26821 tgggataatc agcaactagc agaattagta gaagtaaaac agcaaaatga agcagatact
    26881 tatttaccgt acccaagtta ttaaggagtg acagatatga aatggaatga agtaaaagta
    26941 gaaactgaaa gtgaagcagt cgaagcagtt gctaatattt tgatggaggc aggggcgagt
    27001 ggtgtcgcga ttgaagattc tttagatgtt gaacattttc agtcggatca atatggtgag
    27061 attttagata aaaatgattt tacatcgctg aaagaaggcg cactagtcat ggcttatttt
    27121 ccggaaacga ctttcttacc ggaaatcttg ccttttatca aagatcgtat cacacaatta
    27181 cctgagtttg gtctagctat tggaaaaaat aaagtaacag ttagtgaagt agaggaaagc
    27241 aactgggcaa cagcctggaa gaaatactat catccggtgc gtattacgcg aatgctgacg
    27301 gttgcgccaa gctgggaaaa ctaccagaca gaagatcctc tagaaaaaat tatcacacta
    27361 gatcctggta tggcatttgg cacaggcact catcccacga cttccttgac attgacagca
    27421 ctagaaacag tcttgcgtgg tggtgaaaca ttgctagatg tgggaactgg gtcgggcgtg
    27481 ctaagtattg cagcaaaata tcttggtgca aaagatgtct atgcctttga tttagacgaa
    27541 gtagccgttc gttcggcgaa agaaaatatg gatatgaatg aaatagccaa agatgtccat
    27601 gtatcagcta acgatctttt gacaggaatc gagattgaaa gtgatgtaat cgttgcgaac
    27661 attttagcag atatcatctt attgatgatc ccagatgctt ggcgtttact gaagcaaaca
    27721 ggcacgctga tcgtttcagg aatcattgaa gagaagaagc aaatggttct ggatgccatg
    27781 ttagaacaag ggtttatcgt ggatcaaatt ttccagcaaa aagactggta tgcaattatt
    27841 ttgaaaaaac cagaggtgga atagatgcag cggtattttt taaaagaatc atatgaatca
    27901 aaagactatt ttgttttaga aggggaaaac tatcaccaca ttgtacgggt tatgaggatg
    27961 gaacccgagg atgaagtttt tttggcattc aatgatcaaa tagccattgt tgcagaaatc
    28021 acggagatta ctgaagaggc tgtttatctg aaagaaatta aaaaagaaga aaatcaaaag
    28081 gaactgccaa gttcgattac catagcgagt ggctatccta aaggggaaaa gctggattgg
    28141 atcgttcaga aatccactga attaggagcg caagcttttc ttggtttccc ggcatcagcc
    28201 tctgtcgtta aatgggatgc aaaaaaacgt atgaaacgac aacaacggct tgaaaagatt
    28261 gctcaagagg cagcggaaca atcccatcgg cagatcgtgc catcagtaga gttattggaa
    28321 acgaaacaag atttatttga gcggctagat gattatgacg tggtcttggt tgcttatgag
    28381 gaatcagcaa agcaaggcga aaaatcaaat gcagccaaaa ttcttagtga aacaaaacca
    28441 gatagtcgaa tcttagctgt ctttggacca gaaggtggat tatcccgcga tgaagtcgat
    28501 tattttgtag aacacaaagg gatactttgt ggattaggac caagaatcct tcgtacagaa
    28561 acagcacctc tttatttatt aagtgctgct agctatcaat gggaattaat gaatccttct
    28621 ttcagctaac gttcttcatt aataaaagag tatcgacaga aagaattgaa aaaatagtca
    28681 acaatttgta taatagaaac aattatttat acaatggata cgaaaaatcg tttgaaaaag
    28741 cttttaaaaa gcgaacattt ccgtaaaaaa acgtataact gcacggattt tgggacaatt
    28801 atcaaacaga tatttgtccc gattcccgtt ttaccgaaaa aagaaagggg gatcgattcc
    28861 tgtgacaaaa gaagaaattt taacgggacc cggtgtcata aagctcgtta gtcaatacat
    28921 gggtcccgaa catgtagcat ttgttgagaa agcatgcgaa tacgcgactg ctgcacatga
    28981 cggacagttt agaaaatcag gcgaacccta cattattcat cctatccaag tcgcaggtat
    29041 attagcagat ttaaaaatgg atccccatac agtggctaca ggcttcttac atgatgttgt
    29101 tgaagataca gaaatcactt tagaagatct gagagaagaa tttggcgatg acgttgctat
    29161 gttagtggac ggcgtaacca aattagggaa aataaaatat aaatcccacg aagagcagct
    29221 agcagaaaac caccgaaaga tgctgctagc aatggctcaa gatttacgag ttatcatggt
    29281 caaattagct gatagactac ataacatgcg tacgttgaag cacctgcgag aagataagca
    29341 aagaaggatc gctcaggaaa ctttagaaat ctatgcgcct cttgcacatc gtctagggat
    29401 cagccggatc aaatgggaat tagaagatac agcacttcgt tatctaaatc caaaacagta
    29461 ttaccgtatc gtccacttga tgcagacgaa gagagaagaa cgtgaaaaat acgtaagtgg
    29521 tactgttgaa gatattcgaa tagcgacaga agagctgggg atttttgcag aaatctatgg
    29581 acggccaaaa cacatttatt caatctatcg taaaatgaaa gatcagaaaa agcagttcaa
    29641 cgaaatttat gacttgctag ctattcgagt gatcgtagat tcaatcaagg attgttatgc
    29701 tgtattagga gcaatccaca caaaatggaa accaatgccc ggcagattta aggattatat
    29761 tgccatgcca aaagctaata tgtaccaatc tttgcatact accgtgatcg gtccggcagg
    29821 aaatccggta gaaattcaaa tccgaacaca ggaaatgcat gaaatcgctg aattcggggt
    29881 tgctgcacac tgggcctata aagaaggaaa aaatgaaaaa gtagaaccag atggtatgac
    29941 gaaacaatta agctggttcc atgagatact cgaacttcaa gacgaaagct atgatgcttc
    30001 tgaatttatg gaaggcgtaa aaggagatat ctttagtgat aaagtctacg tcttcacacc
    30061 aaaaggagac gttactgagt taccaaaagg atccggacca ttggactttg catacagtat
    30121 ccatacagat atcggtaaca aaaccactgg tgcaaaagta aatggcaaaa tggtgcagct
    30181 tgattacaaa ttgaaaaacg gagatatcat tgagatcatg acttctccaa attcatttgg
    30241 cccaagtcgc gactggttga aattagttgc tactagcaaa gcaagaaata agatcaaacg
    30301 tttcttcaaa gcccaagatc gagaagaaaa tgtgatcaaa ggccacgaat ccgtggtcaa
    30361 atgtattaca gatctaggat ttacgcctaa agatattttg acgaagaaca aactgcaaga
    30421 agcactcgat cgttttaatt atcaaacaga agatgatctc tatgcagctg tagggtatgg
    30481 agaagttagc cccttgacga tggccaatcg tctgactgaa aaagaacgta aagaacaaaa
    30541 aatcgagcag caaaagcaag aagcagaaga aatcatgaat cagccgaaaa aagaacctga
    30601 caaaatgaaa gtacgtcatg aaggtggcgt tgtcattcaa ggggtagaaa acttactaat
    30661 tcgtatcagt cgctgctgta atccgattcc tggtgatgat atcgttggtt atatcactaa
    30721 aggcagaggg atatccattc atcgtcgaga ttgtccgaat gttcagcctg acaaaccaaa
    30781 tgtagcagaa cgtttgattg aagtcgaatg ggaagataca tcgaatacac gaaaagagta
    30841 tgatgcagat ttggaaattt acggctataa tcgttcaggc ttattgaatg atgtacttca
    30901 aacagtcaat gcgctaacga aaaatctcaa cagcgttgaa gcacggacga ataaagataa
    30961 aatggcgacg atccatttga cggttggtat ccagaattta tcccacctaa agagtatcgt
    31021 ggataaaatc aaagcagtac ctgatgtcta cagtgtacgc cggacgaatg gataggagga
    31081 cagatatgag agcagttgtt caacgggtaa gtaaagctgc tgtgtcagtc gatcagcaag
    31141 aaattggaaa aattggctac ggcctacttg ttttattagg cattcatgaa accgatacgc
    31201 aaaaagatgt ggactatctg gtaaaaaaaa ttgcacaaat gcgtattttt gaagatgaac
    31261 aaggaaaaat gaatattagt gtagaagatg tgggaggaca aattttaagt atctctcaat
    31321 tcacactgtt tgcggatacg aaaaaaggaa atcgaccaag ctttgtggaa gccgcacgac
    31381 cagaaacagc tattccttta tacgaatcat tcaatgaagg aatcagaagt cgcggcatca
    31441 cagtcgctac cggacaattc ggagcagata tgtcggtttc tctcgtcaat gatggacctg
    31501 taacgatcat tattgattct aaaaacaaat aaagtaatct ggttttcact agtaaaaact
    31561 atgaaacacg tcgcttaagt tagactgata aactctaact taagtggctt ttttgtctgc
    31621 aaaaagcgct tcttttgtca gcagtcggaa aaaaagttaa gcttaaatta tacaagttat
    31681 tttttatagg aggttgctga agatgaaaga agtaccagta gtagatatca aaatcacaga
    31741 tttaaaaaaa ttgtatcaaa aaggatacaa catgttggag gagttacgcc atgaagcgga
    31801 cgctcccgtc gtcaaagcaa agattttcaa taaagaagct atcacgatct acggatcatc
    31861 agcagcaaaa gtattttatg atccaaggaa tttcaaacga aaaggtgcta tgccaaaatt
    31921 agtattgaag acactttttg gtcaaggtgg agtacagact ttggatggag ctgctcatca
    31981 ccatcggaaa aatattttta tggatctaat gacaccggaa agaatggaag attatcatcg
    32041 tatcttagat aagaatctga cacaagcact tgaagcacag catggtcaat ttgaactatt
    32101 tgatttaagt aagatggttt tctttacatc tatttgcgag tgggcaggta tcaatttatc
    32161 agcgatttct aaagatgagg tcgaaaaatt agcagaatat caaatctcta tgatcagcgg
    32221 aacgtttact tctccgattg atcacataaa aggtgtcgaa aatcgcaaaa aatcagaaaa
    32281 atgggcgcaa ggcttgattg aagaagctcg acaaaatcct gtggctggaa aagaaaatgt
    32341 ggcattatat gcttttgcca atgccacaga tttagatggt cagcttttac ctctggaagt
    32401 ggcagctgtt gaattattga acatcatccg tccaacggtt gctctaactg tgtgggcggc
    32461 attgatggga catgcacttt tcagtagacc agatttgtat cagcaattaa aaaatgactt
    32521 ttcgacgtta caagatccgt ttattcaaga aatgagacgc tattatcctt tcttcccaat
    32581 gctacctgcg atttcgctga aagaagtaga agtagatggt tatcgtatac cagaaggcag
    32641 ttgggttatt ttagatttat atggaacaga ccatgatgaa cgaacagttg aagcgccaga
    32701 ttcctttatg atcaaacggt atgtagggaa agcaaaagat atttcctata aagaagaata
    32761 tgagatgatt gcacaaggcg ggggaaattt tcgacaaatg catcgctgtg caggagaatg
    32821 gatcactctt catagtctac gtgtgttttc cgatcagcta gtaaataagt ttgagttctc
    32881 tgtacctgaa caagattgga cgattccatt caatcagttt ccgacttatc cgaatagccg
    32941 agcgctactt tacaagaatt agattagcat aaacttatcg aaaaaaagga agatcaaaca
    33001 gtgctgatct tccttttttc atattactat aaattgataa ggtcaattga tcaaaatcca
    33061 cgaaatgctt catcaatggt ttgataatct tcattggata aatgcaaatc taaagcttgg
    33121 acattgtcag atacttgctc tggacgctta gctccaggta taactacaga cactcgtgga
    33181 ttcttcatgt accaagctaa aacaagttgc gcaggagtgg cttggtatct tttagcaatg
    33241 gggcgaagta catcgacagc ggttaaaatt tctctgaaac gttctccttg gaaatctgga
    33301 tttctttttc gaggatctcc ttcaccaaat tgtttttcct caccaagttc atattttcct
    33361 gtcagcaaac cggaagcaag aggaaaatag ggaacaaaac tgattttgtt tttttcaaga
    33421 taagggaata attctttttc agcctgccga tggatcaaac tgtatttatc ttctactaca
    33481 tcgacatatc catctgcatt cgcttctttc aattgttcta acgtgaagtt tgacacaccg
    33541 actgctcgga tttttcctgc ttcttttaat tcgtgcaatg tagcaactga ttcatttttt
    33601 ggtgtgcttt catccggaaa gtggatataa aaaatgtcga tgtaatcagt ttgcagacgt
    33661 tttagtgcat cttccaccga ttgtttgaga aactcaggag aattatcaaa tgttcgccct
    33721 ttatttggta tatgagcagc ttttgtagcg atgacgacac gtgagcgatc atattctttt
    33781 tccttcagta cctcgccaat cagttcttcg gaacggccgt ttccgtaagc ataagcggta
    33841 tcgatcaaat tgatcccgct attcaaagct gtacgaacta cttgttttcc tgtctcatct
    33901 tccaaaccgt caaaaagatt atgtcctcca acggcatttg ctcctaatcc aagctgttct
    33961 gcgtagactt gtgaatgtcc gattcttatt tctgccatag ctgattcctc ctttttgtct
    34021 agtctaaaac aaaataccaa aaagctgaac taaaacgttt ttgtgagggg aaatagtcag
    34081 atatctcttg actttgagac tggtttttag taggttataa gtaatatcga atgtcaatga
    34141 aagagcgagt aagtttttga aactttacaa gagaggatca tcgaggctga aaatgatcta
    34201 aagtttcatt agcctaaaga cactctggag atggacgagt gaaaagccgt cccgcgtacg
    34261 agcgttattc gtaagaggaa agagaaatct ttcaaaatta ggtggtaccg tgtatgaatt
    34321 tcattcaccc ttgttcatga tgaacaaggg tgattttttt atttacatag ctaagaaagg
    34381 gagagaaaac tatgagtttc caaagaccaa aaggaacaaa tgatattttg ccaggcgaat
    34441 cagaaaaatg gcagtttgtc gaagaaacag cacgtttatt attcagagat tatcagtata
    34501 atgaaatgcg tacaccgatt tttgaacact atgaagtgat atcccgaagt gtaggggata
    34561 caacggatat cgtttcaaaa gagatgtatg atttttatga caaaggggaa cgccatgtga
    34621 cgcttcgacc agaaggtaca gcaccaatcg ttcgttcttt tgttgagcat aaattgttcg
    34681 gcccagagta tgcgaaaccg ttcaaaactt tttatatggg accaatgttt cgctacgaac
    34741 gccctcaaaa aggacggtta cgtcaattcc atcaaatcgg agtagaagca tttggatcag
    34801 aaaaccctgc aactgatgtg gagagcatgg tgatggcact tgatttcttc aaacagttgg
    34861 ggatcacgca aatccggcta gtcatcaatt cattaggtga taaagaaacg cgtcaagcct
    34921 atcgtcaagc attgatcgac tatcttttac cttttgaatc agagctgagt gaagactcta
    34981 aacgccgcct tcatgaaaat ccattacgcg tattggacag taaagacaaa cgagatcaaa
    35041 aatttgtggc agaagctcca tctatcttag attatctaag cgagcctgct caaaaacatt
    35101 ttgataccgt tgtttccatg ctggatgcat tggctgttcc ttatgaaatc gacagtaata
    35161 tggttcgcgg actagattat tacacacata cgatttttga aatcatgagt gacgcaccaa
    35221 aaatgggggc acaagcaacg atttgcgcag gcggacgtta tgacaatcta gtggaagaac
    35281 taggtggtcc ttcaacacct ggcttcggat ttgcaatggg tatcgagcga ttactcttga
    35341 ctatggaggc agaggaagta gttatccctg catttaatga gttggatgct tacgtggtcg
    35401 cactcggaga cgaaacaaat atcgaagcat tgaaagtggt ccaagcgatt cgaaactttg
    35461 gtttttcagc tgaccgcgac ttcatgaacc gtaaagcaaa agcgcaattt aagacagcag
    35521 ataaagcgaa tgccaaatta gtattggtga tcggtgaaga tgagcttgct agtcaaacga
    35581 ttaatgtcaa agcaatggca agccgaaaag aaaaaagttt tccattagaa gagatatatg
    35641 aacattttga tgaagtctat gatgaaatga ctttattcga gtaggaggaa aaaaataaat
    35701 ggcacaaaga acgatctatt gcggattagt ttccgaacaa caattagaac aaacagttac
    35761 attaaaagga tgggtacaaa aaagacgaga ccttggggga gtaatcttta tcgacctacg
    35821 tgatcgtgaa ggaatcgttc aaatcgtctt taatcctgaa atcaacaaag aagcatggga
    35881 aatcgcggat aaatgtcgca gtgaatacgt gatcgaagtg actggggaag ttagaagacg
    35941 cggagaaaat gcagtaaatc ctaaaatggc cactggtgaa ttcgaagtga ttgcaagtga
    36001 aatcgtgatt ttgaatcaag caaaaacccc gccatttcaa attgaagatg atcaagcaat
    36061 cagtgacgaa atacgtttga aatatcgtta tttggatctg cgtcgtccaa aaatgactaa
    36121 aaacatcaaa ttaagaaatg cgacaacaaa agcgattcgt cattatttag atgataatga
    36181 ctttctcgat atcgaaacac cttacctagg gaaatctaca ccagaaggcg caagagacta
    36241 cctagttcct tcacgtgtcc atgctggaca tttctatgcg ttgccgcaat ctcctcaatt
    36301 gtttaaacaa ttgctgatga atgctggatt cgaccgttat tatcaaatcg ttcgttgttt
    36361 ccgcgatgaa gatttacgtg gagaccgtca gcctgaattt acgcaagtag atattgaaac
    36421 aacgttctta acagcagaag aaatccaaac ttacacagag ggattgattg cgaaggtaat
    36481 gaaagaagtt cgcggtatcg aagtcacatt gccatttcct cgtatgacct atgatgaagc
    36541 aatggctcgc tacggcagcg acaaaccgga tacacgtttt gatatggaat tgatcgatct
    36601 aagcgataca gtcaaagaag tagaattcaa agtcttccaa atggctcttg aaaacggcgg
    36661 tgtcgtgaaa gcattgaatg ctaaaggcgc tgcagatcgc tactcacgaa aagacatgga
    36721 tcagttaggt caatatgtag gacaattcgg agctaagggg cttgcatggc tgaaagtaga
    36781 agaagacgga ttgaaaggtc caattgctaa attcatggga gaagcaacag aagcaatcat
    36841 taaagcaaca gatgcaaaac caggtgactt gctgatgttt ggcgcagata aaagtgaaat
    36901 cgtcgctgct gcattaggtg ctatccggac acgtttagga aaagaattag gactaattga
    36961 tgaatcgaaa ttcaatttct tatgggtaac tgattggcca caatttgaat tttcagaaga
    37021 agaaggtcga tatgtctctg ctcaccatcc attcacgatg ccaaaagaag aagatatccc
    37081 gttattggct gaagaccctg caaaagtcta tgcagaagct tatgatatcg tcttgaacgg
    37141 ttatgaacta ggcggaggtt ctatccgtat tcatactcgt gagcttcaag aaaaagtact
    37201 agaaactcta ggctttacaa aagaacaaat ggaagaacaa tttggtttct tgttaacagc
    37261 tttagattac ggattccctc cacatggcgg tattgcatta ggattggacc gttttgcaat
    37321 gttattagcc ggagaagata atattcgtga agtcattgcc tttcctaaaa acggaaaagc
    37381 agcagatgtg atgagtgatg caccaagtat ggtttcgcct ttacaattgt tcgaattgaa
    37441 tattgatgta acgaatatag aagaatagcc gataaaaagg taaaaaggat ttgtcgaata
    37501 cgcttctttt gacaaccttg atttaattga aaaacacttt ataatgaagc cataacatat
    37561 aaagcggggc ttttccatga aaaaaacatt cgaaaattgg gcttatcatg attacacgac
    37621 caagctttcg gtatctatcg tttatgcgtt gatcgcatca gtcgcattga atttctttta
    37681 tcagccagga catatttatt caagtggtat tactggtcta gctcagattt tgacgacttt
    37741 gtcaaaatca ctgataggat tcgaagtacc ggtatcaaca actttgtatt tactgaatat
    37801 acctttgttt gtattggcgt ggttaaaaat cgggaagaag tttacaattt tcacttttat
    37861 cacagtcact ttgacctccc tttgtatcca tattttaccg gaaacagtcc tatctaccga
    37921 tccaatcatc tgtgcgttgt ttgggggggc tgtgatggga ctaggtattg ggctagcgtt
    37981 gagaaacggc ctatcatcgg gcggtctaga tatcgtgagt atcacgattc gtaaaaaaac
    38041 aggaaagtcg attggttcta tttcgatcta cttcaatgca ttgatcgttt ttgttgccgg
    38101 aattctattt ggctggcaat atatgtttta cagtacgttg tctattttcg tcagtggaaa
    38161 agtaactgat gtcgtttata ctaaacaaaa gaagatgcag gtaatgatcg taacgaaaca
    38221 tccagaaaaa gtagtaaatg aaatccaaaa ctgtatgcgt agagggatca cgatcatcaa
    38281 tgaagcagaa ggtgcttata agcatgataa gcagacagta ttgatgacga tcgttacacg
    38341 atacgaactg cctctactta gagaagcaat gaaagcttct gatccacaag cttttgtaag
    38401 tattgcagaa aacgtccaga tacttggtcg tttctatgag gatggcattt agatcgagtt
    38461 tgacaaaaat tcatgaaata ctgctgtaga cagacgttcg tttgtctgca gtttttttct
    38521 accatagtag aatcagcaaa gaaaaactgc ttttttattt gaaattttct taaagttgaa
    38581 aaaaagcatg tttatttttt taaatttatg ggatgataga aaaaaatagt agagaaagga
    38641 cacgattcta cttgctattt tcacttaata cccgtatatt acgaatgaaa aaagtttatg
    38701 aaaagttgga atattgaatg gaaaaacaaa aaaggttaaa aaacagccgc tacatcactg
    38761 ggtttgatgg gctccgttca ttagctgtgg tgggtgttat cttctatcac cttctgccaa
    38821 ctagtatgaa aggcggctat ttgggcgttc cgattttttt cgtcgtatct ggttatttga
    38881 tcacggatct gctgcggcag gaatgggaac aaaatggaaa aatcaatatt tggcaatttt
    38941 atgtcagaag aatgaagaga ctctatccgg cacttgtttt tatgcttgtg acagcttcgg
    39001 cttatatcac tttgtttcag cgagggctgt taaacaatct tcgaggagtt gtggtcagca
    39061 gcttactgta tgtcaacaac tggtggcaaa tcaataacgg tttatcttat ttcgatcgat
    39121 ttgctaatga gtcaccgttt acgcatatct ggtctttagc agtagaaggt caaaactatt
    39181 tggtttggcc gatcttgttt gtattgttga tggtatttgt tcgtaaaaga aaatggatct
    39241 tttatactgt gttaggtaca tccttcctct ccgccatctt gatgatgatc ttgtatacac
    39301 caggaggcga tccaacccgt gtttattacg gtaccgacac tcgtctgttt tctatatggt
    39361 tgggaagtgc gcttgcgttt gtttggccaa gtacgcgttt aaagaaaaac atcccgaaac
    39421 aagcgaaaaa tattctgaat atcgctggag gaatctcgct tttggcactg attctttgct
    39481 tcttcttttt agatgatcat tacaatttca tctattacgg aggaatgctc ctagtgagtc
    39541 tgttatgcgt agtgctagtt gcagtgacag cacaccccgg agcaagtttg aatcgatggc
    39601 tgacaaatcc agtatttact tggattggca aacgaagcta tggcatttat ctgtatcagt
    39661 ttccagttat gattttttat gaagcaaagg tgactgatat tgccgatcat gttttgttgc
    39721 atactttagt ggaaatcaca ttgattctaa tcatcagtga actttcttat cgatatgtgg
    39781 agcgtccgct agcacgtttt gattatggtc gtacattcca agtgatgaaa ggatggttca
    39841 gcaaaccgat catcagtaag agcaaaccat gggttgttcc aggcacatta cttgtagcaa
    39901 ttgcactcgt tggtttcgtt acggcaccga aaaacgctgt aacagccgat caaaaacaac
    39961 tgcaggcaaa aatcgctgaa agtaaaaaat tagcagaaga aaccagaaac aattcagaaa
    40021 acaccactag ttcggttgac caagcgatcc ttgataagta cgagttgtcc aaggcacaag
    40081 ggaataaggc acaaaaatta gaactaacgg catttggtga ttcagttatg ttggatgctg
    40141 caacggatct acaagaactt tatccaaaag tagtggtaga tggagatgtc ggacgacaat
    40201 tatatgccag tgtaccgtac attgaaaaat taaaggaaca agatttgttg aaagacactg
    40261 ttctgatcgg actaggaacg aatggctcat tctcggaagc tcagtttgat agtgtgatgg
    40321 atgcaatcgg tgatcgaaaa gtatactggg tcaatgtccg agtaccgacg aagcgctggc
    40381 aaaatgacgt gaatgcgatg cttgaacaga tgacgaaaaa atacgacaat atgacgctga
    40441 ttgattggta cagctacagt aatgaccata gtgactggtt ttatgatgat caagtccatc
    40501 caaatcctga agggatgaca cattacattc acttagtcag cgaagccttg ctaggaggaa
    40561 gttcaagtac gaacagcagt gagacaaacg tgacagaatg atagctacaa aaaacttcag
    40621 gcaaaaacat ttgtttttgc ctgaagtttt tttatattta ccgtgttttt aacattcata
    40681 tagattcagg aagcaaaacg gttgagtgtg tctgtaaata acgttctttt tgttttcggg
    40741 tgagtttttt gaatgcagct tctgcttttg ttgcttcgct tcttgaagaa aatacttctg
    40801 catggatcat cttaacaggt cttctttttt gaagacgagt atatttagca ccagtaccat
    40861 tgttatgttc ctctaatcgt ctagaaagat cggttgtata tccaccataa aatgtctgat
    40921 ctttacagaa taatacgtaa aaataatgtt catttgccat agagcatctc acgaatctct
    40981 gcagtgtatt ctccatccat attgtaagtg atcaatggtg gagcgacttt gaatccgtct
    41041 aatttgcctt ctttgattcc ttcaactaac aaaatatttg cctcttttcc aggttttggg
    41101 taaacaaatt ggatcctttt aggtgcaatt cgatagtctt ccatgacctg aatgatttct
    41161 aagaaccgat caggacgatg gaccatagta aagcgtccat ttgtttttag tagcttactt
    41221 gaaatatcga tcacttcttc taacgtcgta tgaatctcat gacgggcgat tgccaggtgt
    41281 ttattagggt tcgttttatt tgttggcagc cctttgaaat aaggtggatt acagacgact
    41341 aagtcacaag aattatgtcg aacgacagat aagctgtctt tcagatcgat ggtgtggaca
    41401 gtcatttgtt catttaaatg gttaagcgcg atactacgtc ttgccatgtc tgctagacga
    41461 ggttgaagtt cgatagcgtc gatagccgcc tgggttcgtt tacttaaaaa aagtccaact
    41521 gcgccatttc ctgcacaaag gtcaacaatc ttcccttttt taggtacacg ggaaaagtga
    41581 gcaagcagca cagcatctaa agaaaaagaa aacactttgg aactttggat aatcttgata
    41641 tcttctgcgt ataattggtc gattcgttcg tcttcgtgaa gcatggattc cctccaaaat
    41701 gaaaaaaata gtcgtctgta ttatactatt tttattaaaa agaaccaagg tggcttgtca
    41761 aacctctatt ttttatgcat actatgacca taagaaggga aaggaagaga ctatgtttta
    41821 tcgatttatg cgcggggtag ttcgagtact attagcctta gttaatggaa atgcccgcta
    41881 tgagaataaa gaagttttac cgcaagatga gaattacata ctagttgctc cccatcgtac
    41941 ttggtgggac ccattatatc tggcggtagc tgcagcaccc aaggaattca gctttatggc
    42001 aaaagaagaa ctgttcaaaa atccagttct gcgatttata ttaactcgat ccaatgcttt
    42061 tccagttaaa agagaaaatc caggaccaag cgtcattaaa acacctgtaa agattttgaa
    42121 atcgacaaat ttgagtttga tcatgtttcc cagtgggaca agacatgcta cagaacttaa
    42181 aggcggaatg gcattgattt ccagaatgtc taaagcgaaa atcgtgccag ctgtatatca
    42241 aggtccgttg acacttgggg atctgttcaa gcgtcagaga gtgacagtgc gtttcggcga
    42301 accaatcgac ttatcagata tcaagaaaac ggataaagaa ggtatggcgg agatcgaacg
    42361 acgaacacaa gcagcttttg acaaattgga caaagaagtg aaccctgatt tcaaatatga
    42421 agtcaaaaaa aaataaaatg ataagtttat gcgtgggcgg tcttttgaat ctactgtcct
    42481 gttcagttca gactttttca atgaaaacga gggagagacg tcaagtctgc cctctttttg
    42541 cgttccaata tgtaaaatag aagacaggtg tcatgtaatg gatcaaagtg ttataataat
    42601 tagaaggaca ttttttgagg tgattgaatg cgttttttac atacagccga ttggcatatc
    42661 ggaaaaaaac tacaaggtta tgacttgatt gaagaccagc gtcatgtact atcttctata
    42721 ctagatatcg cgaaagaaga aaaagtagag gcaatcgtga ttgccggaga cttatatgat
    42781 aggagtgtcc cagctgttga tgccgtagaa ttatacaatg aattgatggt cgattggaat
    42841 ttaaaggaaa agatcccgat ttttgctata tcaggaaatc acgatagcag tacgagactt
    42901 tcagcgggta cgccatggtt cagttattcg aactattttt tgcgaacaca gctagcagaa
    42961 gcatttgaac cagtcgagtt taaaaacgta caatttttct ttcttcctta ctttgaacca
    43021 attgcagcga gattatattt tgaagatgac aatatccgaa cgatcaagca agcggtagcc
    43081 aaggtgatag ataaaatgca ggagctattt gattcagaaa aaaaacaagt attgattagc
    43141 catttttttg taatgggaag tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg
    43201 ttggattcga tccctggtga actacttaca ccgtttgatt atgtagcact aggacatttg
    43261 cataatcaag ctgctttgag acaggataat gctcgctata gcggttcccc attgaaattc
    43321 tctttatctg agatcaacca aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg
    43381 gagttgaatt ttagggagat cgaacccctg catgatattg aagaaataac agcgagtttc
    43441 aaagaattgc tggatcctgc cttttatcag tccgtcaatc gggagaatta catctatatc
    43501 tatctcactg atagaacgat catcccgaat atgatgaacc agcttcggca aatatatcct
    43561 cgtattttgg ggattgaacg ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa
    43621 gtactagaaa tgaaagatcc tcaaaaatta gcagaagcat tttttagtga aatgacggaa
    43681 gagcagctaa ctgcaaacca agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa
    43741 atggaatgat caagaatgaa accgaaaaaa ttacaactta aaaattttgg tccttttatc
    43801 aatgagaccg tagatttttc acgtttgaca gaagcaccgc tttttttgat cagcgggaag
    43861 acaggagctg gcaaaacgac gatttttgat gggatcacct tcgcactttt tggtgaaact
    43921 tccggaagac ttcgctctgg aaaagagatg cgttctttgt ttgctacacc agacgaagaa
    43981 acaagcgtga ctttttcttt tgaacatcag cagatgagct atgaaatcga acgaaaacca
    44041 gaacaagttt tagcaaaacg aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg
    44101 ttgaccattt ttgatgggaa aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca
    44161 gatcagttga tcagagaact gatgaatctc gatgcgaaac agttttctca aatcgtttta
    44221 ctaccccaag gagaattccg taattttttg gtttcatcaa gcagtgataa agagactgtt
    44281 ttacgtcatt tattcggtac tcaatttttc caacagttca atgagcgatt aaaagaaaaa
    44341 gcaaaacaac agcaacaaaa attggatcat ttagagcaag agctgcatct tctgcaaaaa
    44401 aggtttgttt tagtcaaaga agaggaacca gcagcagggt ttgaagccgt tttagcccaa
    44461 tggacaaacc atcaggcaat actcgaaaaa caaatcacgg aagaaaaaga agctctaacg
    44521 gaacttcagg aaaaacaaaa gcaactagaa aattcctact atcgttttga aagcttgaaa
    44581 aaaagttatg aagaaaaaac gatattaata aacaagcaaa aggaattagc tgctcaaaaa
    44641 gacgaaatag aggaaaagaa acgatggatt gggtattatg aatttacgca acgattagtc
    44701 gaaccaatcc agcgcctaca agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt
    44761 agtgagcaaa caaagaaact agcgatgact acgcagaatt atgaagagtg gcaaaaaaat
    44821 gaagcaaacc gcgtacgccg acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac
    44881 aaacagatga tggtgccaat tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa
    44941 ttaacaattt cattggataa agaagaattg aatgccgaga agctgtatca aaatctcgct
    45001 atctatcagg aaagaactcg aattcttcaa gaaaatttac tggatcagga aaaactgcaa
    45061 gaagatcgtc ttgcttttgg acagttaaaa tatctagatg aaaagcagca agaaatagaa
    45121 aaacaaaaag caaaagacac tgggtcgctc gcacagataa gagaagaact caaaaaagca
    45181 actgaagaaa taacaaaaaa tagtcagcta ttgaaagaac agacagaaca agcagaacag
    45241 ctgaaaagcc aatgggcaaa gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg
    45301 gagccatgtc ttgtttgtgg atcaacggaa caccccatac aacaattaaa acacgaacag
    45361 ccaaataagg aagatatctt aaaagtagaa caacaactgg cagaagctga gtctgctgta
    45421 gaaagaactc agcaaatcat tgccaaaagt gaagcacatg aaaataatct tcaacaacaa
    45481 ttatcagatt tagaacaaaa aatcatggca acgaatatac aattggctga acaagaagaa
    45541 cacttgcaac aggctttgaa aaaggcattt cctgagataa cagaacaaaa aacaaaaaca
    45601 actcttgtag taatcgaaca atctttagat agacgaaaaa gcaagatggc caatgcaaga
    45661 atagagctgg aagaaataag acaacaagca gaaaagctcg aacaacaatt agctgtctac
    45721 aaacagcagc agacagaaaa aaataatcag ctccagcaac tgcaaggaga aatcaaaagt
    45781 ttagaacaac aattgcaaga tacggatagt aactgtttag aagaggagat acgacgactt
    45841 gaaaaacagt tagaagaaga ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa
    45901 ttgttgcaag atcttcatac gcttcggaca cagcttgatc ttcaacatga acaaatagaa
    45961 aaaatagatg aaaaaataga aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag
    46021 tcgtattttt caacaattga agaaatctgt gcatttgcac aacagcaagc acaatataaa
    46081 gaaaaccaac gtacagttac ttcttatcat gaagagcaat tagtgacagc tgaccggctg
    46141 cgtcaattag aaaatatcca gctaactgag acattcccag atacagaaga gatcagccaa
    46201 cagttagaag aaataagacg tatgatcgaa ataaagcaag aaaagatata cggtttacaa
    46261 gaacaaaaaa ttagtaatca aaaaatctac gatgaatgtc tttcgattta tcacacaagc
    46321 cagacgaaat tagatgagct tacgcaatta cagcagcttt cgcaaacatt caacggagaa
    46381 aatcctaaga agactagttt ggaacggtat gtgcttcagg tttatcttca agaagtgtta
    46441 caagtagcaa atgatcatct ccaacgttta acgaaaaatc gttatcaatt tgagctagcg
    46501 gataccattg gcagttatcg agggaaaacc ggattagaaa tcaatatcta tgatgatcag
    46561 gcaggtatgt ctcgaagtgc gcataccctg tctggcggtg agagttttat tgcagcactt
    46621 tctttagcat tagctcttgc cgatgtgatc cagacacaag ctggtggtgt agcaatcgat
    46681 gcattgttta ttgatgaagg atttgggtca ttagatgaag aagctttgga aatggcaatg
    46741 gaagctttag aaacgatcga aagtgaagga agaatgatcg gcatcattag tcatgtaaga
    46801 gaattgaaag aacgagtatt acaacaaata agaattgata cagaaggtag cggacaaagc
    46861 aaaatacgct atcaacttgg ataaaaaggg agattggtaa ctatgaaatg gagtattcca
    46921 gaaaaagtca ttgcacgtgg acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc
    46981 cctgaccaag aagaaaacgt gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt
    47041 gatttggatg gtacagcaaa agagatagat tactgccaat gtccctattg ggaggaacat
    47101 aaatattgta agcatacagt tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt
    47161 atcatgaaaa aagaccagcc aatgctagaa ccggtaaaag caacaagtga gggagaaatc
    47221 attgcaaaag gtttttctcg tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg
    47281 attattgaat accaagtaga aacgattgag actaatcagt atcacccaga gctttctatt
    47341 ttggcacttt acttgcgtat cggttcattg gcatcaccga aaaaaacata tatcgtaaaa
    47401 aacatctatg attttttaca agcttacaca gaaaaagaaa cttacactgt taataaacag
    47461 taccagtttc gtctagataa acgggcgttt caaaaagaag atcaaaaaat actcacgcat
    47521 cttgcaggaa gtgctcagac gcatcaatta ttaggtacga acggcttaca agtcaaagga
    47581 aaactagata aaagatatct gcttctccca gtagaacaag cccgcttcct gttagagcag
    47641 atgaatcaga ctacacgttt gcatctgcag ataaatgaac aaaacgatca aggaatacaa
    47701 ttttccgatc aaaacaaacc tttatcattc aaagtggaaa aatttggaga agattatttg
    47761 ctgcaagcat tgaatgattt tgattttttc tttatgcatt atcagtgggg agttatccaa
    47821 gggacatttt attctctgac gaaatatcaa caaacggttt atcaaacttg gaaacaattg
    47881 attcgccgat tggagaaacc agaagtagtg ttcaagaaaa aagaattacc gtctttgttt
    47941 aaagagatta tccctctgct gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa
    48001 gaggaagtag cagatatccc cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa
    48061 atccaagctc gagtcgattt cgtctatggc gaagtcatct attcgactga cccgaagcat
    48121 gaggtctcga cagaacaggc ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta
    48181 cttgatctat tcagaatgta tcgctaccag aaaaatgaaa ctggttatga acgtccattg
    48241 ccaagcgggg aagaactata tgcctttttc cgcacagaac tatctgtctt ccgtcagttt
    48301 ggtgaagtca gacttggaag aaaattgcga gagttgtatt tagatgcgca taaacatcaa
    48361 ccgaaaatcg aagtaaccga gaccggttca tggctcgata ttcgattcga tgtgacaggg
    48421 atagaggagc aagaaatcga tcatgtcctg caaagcttgc tgagaaatga tgctttttat
    48481 acattagaaa atggtcaggt tctttctttt gattcagaag aatttcaaca aacaagccaa
    48541 gtgttgcaac aattacggga atcgatacgt acagaagaag gaacgatcca tgtacctaaa
    48601 aatcaaggat tgatcattca aaatcaattg gaaaaaagca atgctacttt tagtgaatcg
    48661 ttccaaacga tggtacaaga tctcattcat ccagaaagat accaggcaca gcttcccaaa
    48721 ggattgaatg caacgatgag agattatcaa aagcaaggtt ttcgatggtt gaagatgtta
    48781 ggtcattatc agtttggcgg tattttagcc gatgaaatgg gacttgggaa aacactacag
    48841 acaattgcct ttttactttc ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta
    48901 gcgcctgcga gcttaattta caactggcag gcagaggtga gaaaattcgc cccttcttta
    48961 agtatccaag taatcaatgg gaataaaaaa gaacgtgaag aattactggc taaagacaca
    49021 gatatccgag tgacttctta tgccagctta agacaggatc tagctaatta tcagtcgcaa
    49081 aaaattgatt atttgattct tgatgaggca caaatggtca aaaatagcag tacgaaaact
    49141 gcacaagcat tgagagaatt agctgtacct cagcggtttg cactgagtgg gacaccaatc
    49201 gagaataatt tagaagaatt gtggtcattg tttgcaacga ttatgccagg atttttcccg
    49261 actaaaacaa aattcaggga acttgccgca gaagaaattg cgcaaatgat tcgaccgttt
    49321 attttacggc gagataaaca aactgtgttg aaagacttgc cggaaaaaac agagatgaat
    49381 ctatattctg cacttacaga agaacaaaaa actgtttatt tagcttactt gcgtcaaatg
    49441 cgtgaagaaa tcacatcaat ggattctgaa gcattcaaga aaaaccggat cgggatactt
    49501 gctggtctga caagactgcg acaaatttgc tgcgatcctc gtctgtttat tgaagattac
    49561 caaggcggtt ccggcaagct cgaacaagta aaagatctac tggtagctgc caaagagaac
    49621 aaacgaagag ttttgctgtt ttctcaattt acagggatgc tgactatctt gcaagaagag
    49681 ttagccgagc tagggatctc gacattttat ttaagaggca gtacgaaacc acaagatcgg
    49741 ttaagtatgg tagacgcatt taatgctggc gaaaaagatg tttttctgat ttccttaaag
    49801 gctgggggaa ctggattgaa tctcacggga gcagatacgg tcatcttgta tgacctttgg
    49861 tggaatcctg ccattgaaga acaagctgct ggacgtgcac atcggatcgg acaaaaaaat
    49921 gtggtagaag tgtggcgtat gatctctgaa ggaacgattg aggaacgcat ggattcactc
    49981 cagcaagaaa aacgcgaact gttccaaaaa gtgatccaag gtaatgaaga acagttgacg
    50041 aaactgactg aagaagacat ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag
    50101 actcgaaaaa gcgttcagta atgggtaaga aagcctttct aaagaagcga ttgtttattt
    50161 gatttaaaaa atagcaagga ggctgggatt ccattttatc ccagcttcct tgctatcatt
    50221 tttttatttc tatttttaaa ggttatacaa agattgtctt cattgtctct aacagctgat
    50281 caaaagaaga aatctcgata tcgcaaattg gagtctgttc aattttgcgt tctcgatggt
    50341 tgaaccaaag cgcttgccag ttagcacttt ttgcacctag aacatcattg tcgtaattat
    50401 cgccaacgta taaggtctct tctggaagca tatgaaattc tttctcagct agctgaaaaa
    50461 tctctttttc tggtttttgg aaacctgttg cttgagaaat gagcatattg cgggaaggaa
    50521 tccaattgtt caattgaagt tgcttcagtt tttttgtctg atggtcagtc ggcccatttg
    50581 tgataattcc taatgggaca ttcttttcct tcaagaaatc gagggttttt ttgacctcct
    50641 catgcaagca gatattatca agttcttcct cgtaaatttt ttgaaataag aggccatctt
    50701 cttccgtaat atgagggtag tctaaatctt ttagtgattg gctgatccga tgtgctcgca
    50761 tatattctag tgtccaatct cctgccatta cttttgggaa gttttcatca ctatgatggc
    50821 ggaaccggat atatagtgga tgcatatctg catctgaaac aagcggaacg actcttttga
    50881 ccgcgtttcg aaatggctgc tgctgatcat acatcgtatc atcaacatca aacacaactg
    50941 atttaatcaa aattttacac gcctttcggt tttcattgat tttttcacaa atccttgact
    51001 attgtaacag aaaaaattgt ctaaaaaagc tgataaataa aggtttttct tactatattt
    51061 ctatttcctt gacagaataa ggtttttata tttcagtttt tttcactaaa aatcgtgaaa
    51121 cacgtcaata agtttgatga acaaattaat cattatggta gaagtcgatt gatttatttt
    51181 aatgcaatct ctgtggaatt ttcgaaagat ttattttttt aatacagtct caatataact
    51241 attcaaacat aaaataacgt tatttgattt atttgtttat agtaaatttt atttagtaat
    51301 cattgcttta aaatactacg cttatataac agagaaaata agcgttataa taaagaacag
    51361 tattatttaa aatgacagaa aacattacta ttaaagaaat aaaaaacata aaaaatctta
    51421 agaaatcggt ttcatttagc ttttgagata aaataatagt acagttttat acgctgtttc
    51481 atatgtttag aaaatttgaa ttagatttga aaactctaat ttttaaatga taaaaaaagc
    51541 gagaatgctg gttttactgt atattaacct tagataactt tcattttttt aaaaaaatta
    51601 tgaaaaaaag gtagctattt aaaagttgat tatgtataat agaaacagat gggagattat
    51661 tatgatcttc tgttacaaaa aagtgattct tatcacgaaa aggagtggtg cacgttgtta
    51721 acattatata cttccccaag ttgtacttcc tgcagaaagg ctcgtgcgtg gttgcaagaa
    51781 catcagattc catttgttga acgtaatatt ttttcagaac cattgaacag ttctgaatta
    51841 aaagcgattt tacaaatgac agaagacgga acagaagaaa tcatctcgac acgttccaaa
    51901 gtattccaaa aactgaacat ggatttagat gatctgccgt tacaagaatt attggaactt
    51961 gtgcaaaaca atcctggatt gcttcgccgt ccaatcatga ttgatgataa acgtctgcaa
    52021 gttggtttta atgaagatga aattcgtcgg ttcctaccgc gcgatgtacg acaattagaa
    52081 ttacgtcaag cacaattgat ggctggatta tagaaggata atacgaaact tggaaaagac
    52141 tattacaaag atagtttttc caagtttttt tgtttttaca aaaagtattc ttgattagat
    52201 gtataaaaag gtagtagaag atgatgattg cacgaaaaat agagagggaa aattagctga
    52261 aaagtcggct atatagtatt cgtctgccaa aaatgttata gttaaataag atagcttgaa
    52321 aggttgaaaa atccgctggt tttattaaat tcgcttaaca gcgagatttt ttttctttac
    52381 atttaagctg atttcgctac aatgaatcta tattaaacat tgtggcaaag tgaggtgtag
    52441 caaaatggaa atggaacata ttaatgaaaa tacgatccgt gtactaattg gtcatgaaga
    52501 tttagaggaa cggggcgttt catttttgga tttacttggc aatcacaaag aaatcgaaaa
    52561 tttcttttat agtattttgg aagaagttga tatcgaagag gagttccgag gaagcgaagc
    52621 tgtcactttc caagttttgc caaaaggaga cggattagaa ctgtttatca gtaaaaatct
    52681 tccggatgaa gaaatttctc aatcagaaga ttctactgtt gattcagaag atgtgagtga
    52741 ctttcttcgt cagcagatta ttggcagcga tccggaggaa gaattatctg gaagcgccct
    52801 agaagaacag gaaagacaag caacttttgc ttttgaagat tttgaacaag tgatccaact
    52861 tgcaacagat gtcgaactag agtctgcttg gactgatttg tatttattcg aagagcgcta
    52921 ttacctagtc gttcacttct ggatggaaaa tctcaaccaa gcggatgtag agaatcagat
    52981 tgctcgaatt ttggagtttg ctgaaaagag cgatcgtaca ccagaaacat tgaatgaata
    53041 tggacaatgt ctgatggcgc gtaatgctat cgagcgaacg aaattttatt ttgtataaat
    53101 cagcattata gtaagagact gtgacaaaaa ttgtgtcgca gtcttttttt cgtataaatg
    53161 tgttcaaaag cttgcatctg cggtaacaag cccaaccaaa cgaaaaatat tgggaactat
    53221 ttttgtttag ctgttcttat tacttgatgc agaatagctt tttcacgccc tcttttttcg
    53281 tgtaaataaa ttcttagacg taaataagtt cagagggaga aaaatgctgc ttgcaatgaa
    53341 tgaatataat aagtgctgtt ttgctaaaca tgcagaaaaa ggaaagaaat attattgtcg
    53401 tggctgtcga aaaagagtga ttttgaaaaa gggaaagaaa aaatgtgctc attttgccca
    53461 tcaaaaatca gataactgtt cggtctttag tgaaagagaa tcaaaagagc acttacagtt
    53521 aaaggaatgc ttcatggatt ggctgggaca gtcagtagaa ccagtatttt tggaagccta
    53581 tttgccgagg ttgcggcaaa ggccggatat tttgttggcg aatctagcaa tagaaatcca
    53641 atgttctaga ctgtctcacc agagatttat tgaaagaacc caaaattatt tgaataacag
    53701 ttaccaagta tggtggatac ttggtaattc ttttcttgga caatcgcaat tctcattgat
    53761 agaaaaaagt tgctgctact ataatagaaa aagaggagtg cattgctgga aagctgatct
    53821 caagaaacaa aagctttatt tgtatcatca tattacagag actgtatcag ggcatatttc
    53881 cttttgcagt agctgctgga ctttttctag tagggacctt aaagaaatat tcactagtaa
    53941 cgaaattaaa atgaaccaaa tgaaaaaaat agaaaaagtg agtgaagatg ggaagaaatg
    54001 gctgacacgc caacttattc acaagcaaaa gaatacagta tccatacaag aacaatgcta
    54061 cttgcgtcat aaacatcttt tgcatctatc atcgtggatc tatcaaaaaa gtcggttttt
    54121 cttttattta caagaacagg tttttcttta tcgaatgttg tacgaagaag ccttggaaaa
    54181 ccagaaagtt cctgatttca actcatggct ttgccaaata aaagaacata aaagggaatg
    54241 gctttttcct atgattgatg aagagatggt ctatcagcag ttttttaacg aatgcataca
    54301 cttgtcttct ttgaaaaaat agtaagtata aaaataggct aggaatcggt tttctatgta
    54361 aaacaagatt tagttatgct aaaattgatg gaagcagaca catgaatgag tagaaaagtt
    54421 agatgctgtg acatgtggat gttagactat gatctatttt gatagataga gctaagggaa
    54481 gaaaaggagt ggatttcatg gaagtaaagc agttgccaaa acgagaagaa ttgcctgaaa
    54541 atttaacttg ggacttgacc aagatctttt caagcgacca agagtttgat gagaaatatt
    54601 tggaattatc agaagagtta aaacaatctg aaaaacacaa aggaacactt gatcaaggcg
    54661 cttctcaatt tttaaatgcg attgaattcg tattgagggt ttatcgccaa actgaagtca
    54721 tttatgtata tgcgcacctt aaaaacgatc aagacactgg aaatacagat taccaagcgc
    54781 tttatgcaag agcaagcagt ctgttttcga aagttagtga agccgtttcc tggtttgaac
    54841 cagaaatatt gcaattgtca gatgaccaga tttggcaata tttcaaagaa gaaccaaaat
    54901 tggaagtcta tcgccattat atccagcaaa tagtagataa tcgagcccat gtcttatctg
    54961 ctgagcagga atctcttctt gctggagcag gtgaaatctt tgatgcttca agtgatacat
    55021 ttgctgtttt gaataatgca gatctagttt ttccaacgat tgaaggagaa aatggtgaaa
    55081 tagtccaatt atctcatggc gtgtatggtc agttgctaga aagcacggat cgaagggtgc
    55141 gcgaagcagc atttaagggg ttgtacagtg tttacgaaca atttagaaat acatttgctt
    55201 ctactttagg cacacatata aaaggacata attttaaagc gaaagtccgt aattacagct
    55261 ctgccagaga agcgtctttg agcaataatc atattcctga aagtgtatac gatactttgg
    55321 tagacgtggt aaacaagcat ttgcctttgt tacatcgata catggaatta cggaaacgtt
    55381 tattagaagt ggaaaaactg cacatgtatg atctttatac accggtctta ggggaagctc
    55441 caattacctt tacgtacgaa gaagcaaaag aaaaagcttt agaagcactg aaaccaatgg
    55501 gtgaagaata catggccatc gtagaaaaag cattctctga acgttggatc gatgttgtcg
    55561 aaaataaagg gaaacgaagc ggtgcttatt cttcgggaag ctatgacaca aatccatata
    55621 ttttattgaa ttggcatgat acgctggatc agctatttac gcttgtccac gaaatgggac
    55681 atagtgttca tagttatttc actcgttcga accagcctta tgtgtacggc gactactcca
    55741 tctttttagc agaaattgct tcgacaacga atgaaaatat cctaacggag tatttattgg
    55801 aaacagaaaa agaccctcgt gtacgggctt atgtactcaa ccattacttg gatgggttta
    55861 aaggaacagt tttccgccag acacaattcg ctgaattcga gcatttcatg catacggaag
    55921 atgaaaaagg tgtgccattg actagtgaat acctaagtga tagttatggt aaattgaatg
    55981 caaaatatta tggtccagca gtcgaagaag acccagaaat taaattcgaa tggtcacgga
    56041 taccgcattt ttattataat tactatgttt tccaatactc gactggcttt tctgctgctt
    56101 cagcactggc gaagaaaata ctaaaccagg aaccagaggc actagagaac tacttggctt
    56161 acctaaaagc aggtaacagc gattatcctg tggaagtaat gaaaaaagca ggagttgata
    56221 tgacacaggc tgcatatatt gaagatgcaa tgtcgatgtt tgaacaacgt ctaaatgaat
    56281 tagaagaatt gatcgatcgt ttataaggct ttctattttt gattttctcg aatagaaacg
    56341 aaaaaaatac gtcggctctc ttgtaaggga gtcgacgtat t
//
LOCUS       DASHFF010000013        51801 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.013, whole genome shotgun sequence.
ACCESSION   DASHFF010000013 DASHFF010000000
VERSION     DASHFF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.013"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            164..2698
                     /gene="secA"
                     /locus_tag="U2I75_000889"
     CDS             164..2698
                     /gene="secA"
                     /locus_tag="U2I75_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN1696040.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            2825..3926
                     /gene="prfB"
                     /locus_tag="U2I75_000890"
     CDS             join(2825..2896,2898..3926)
                     /gene="prfB"
                     /locus_tag="U2I75_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN1696041.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4031..4717
                     /gene="ftsE"
                     /locus_tag="U2I75_000891"
     CDS             4031..4717
                     /gene="ftsE"
                     /locus_tag="U2I75_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN1696042.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4701..5594
                     /locus_tag="U2I75_000892"
     CDS             4701..5594
                     /locus_tag="U2I75_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696043.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5746..7194
                     /gene="cls"
                     /locus_tag="U2I75_000893"
     CDS             5746..7194
                     /gene="cls"
                     /locus_tag="U2I75_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1696044.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            7531..8388
                     /locus_tag="U2I75_000894"
     CDS             7531..8388
                     /locus_tag="U2I75_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN1696045.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8503..9423
                     /gene="pstC"
                     /locus_tag="U2I75_000895"
     CDS             8503..9423
                     /gene="pstC"
                     /locus_tag="U2I75_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN1696046.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9423..10307
                     /gene="pstA"
                     /locus_tag="U2I75_000896"
     CDS             9423..10307
                     /gene="pstA"
                     /locus_tag="U2I75_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN1696047.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10322..11128
                     /gene="pstB"
                     /locus_tag="U2I75_000897"
     CDS             10322..11128
                     /gene="pstB"
                     /locus_tag="U2I75_000897"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1696048.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11144..11902
                     /locus_tag="U2I75_000898"
     CDS             11144..11902
                     /locus_tag="U2I75_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1696049.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11915..12592
                     /gene="phoU"
                     /locus_tag="U2I75_000899"
     CDS             11915..12592
                     /gene="phoU"
                     /locus_tag="U2I75_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN1696050.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12814..13848
                     /locus_tag="U2I75_000900"
     CDS             12814..13848
                     /locus_tag="U2I75_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1696051.1"
                     /translation="MQKKISIIITLFFICILASWFIQRQAALYDVPVIEIERVRIEED
                     QQLITGRLMNTDQKGEVISLTAPYQKNQIETANITTRQIYLVQMHGDEWQLVAKKNDG
                     WLFFFTSVFIGTMLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAILFV
                     VIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTR
                     PYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVIGS
                     MANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIAVL
                     FFLAVRRLKK"
     gene            13845..14612
                     /locus_tag="U2I75_000901"
     CDS             13845..14612
                     /locus_tag="U2I75_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1696052.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            14712..16280
                     /gene="liaX"
                     /locus_tag="U2I75_000902"
     CDS             14712..16280
                     /gene="liaX"
                     /locus_tag="U2I75_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN1696053.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENSEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16456..16764
                     /locus_tag="U2I75_000903"
     CDS             16456..16764
                     /locus_tag="U2I75_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN1696054.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            16764..17114
                     /locus_tag="U2I75_000904"
     CDS             16764..17114
                     /locus_tag="U2I75_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN1696055.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            17280..18218
                     /locus_tag="U2I75_000905"
     CDS             17280..18218
                     /locus_tag="U2I75_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN1696056.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18233..19063
                     /locus_tag="U2I75_000906"
     CDS             18233..19063
                     /locus_tag="U2I75_000906"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN1696057.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            19107..20132
                     /locus_tag="U2I75_000907"
     CDS             19107..20132
                     /locus_tag="U2I75_000907"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1696058.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20147..21085
                     /gene="galU"
                     /locus_tag="U2I75_000908"
     CDS             20147..21085
                     /gene="galU"
                     /locus_tag="U2I75_000908"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1696059.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            21210..21659
                     /locus_tag="U2I75_000909"
     CDS             21210..21659
                     /locus_tag="U2I75_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN1696060.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            21652..22254
                     /locus_tag="U2I75_000910"
     CDS             21652..22254
                     /locus_tag="U2I75_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1696061.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(22317..23420)
                     /locus_tag="U2I75_000911"
     CDS             complement(22317..23420)
                     /locus_tag="U2I75_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1696062.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            23630..24631
                     /gene="ccpA"
                     /locus_tag="U2I75_000912"
     CDS             23630..24631
                     /gene="ccpA"
                     /locus_tag="U2I75_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN1696063.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            24985..25380
                     /locus_tag="U2I75_000913"
     CDS             24985..25380
                     /locus_tag="U2I75_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696064.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            25377..26633
                     /locus_tag="U2I75_000914"
     CDS             25377..26633
                     /locus_tag="U2I75_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEN1696065.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            26645..26968
                     /locus_tag="U2I75_000915"
     CDS             26645..26968
                     /locus_tag="U2I75_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696066.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            26961..27227
                     /locus_tag="U2I75_000916"
     CDS             26961..27227
                     /locus_tag="U2I75_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696067.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(27390..29735)
                     /locus_tag="U2I75_000917"
     CDS             complement(27390..29735)
                     /locus_tag="U2I75_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1696068.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            30014..30916
                     /locus_tag="U2I75_000918"
     CDS             30014..30916
                     /locus_tag="U2I75_000918"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN1696069.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            31127..32902
                     /locus_tag="U2I75_000919"
     CDS             31127..32902
                     /locus_tag="U2I75_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN1696070.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
     gene            complement(33211..34797)
                     /locus_tag="U2I75_000920"
     CDS             complement(33211..34797)
                     /locus_tag="U2I75_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN1696071.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            35048..35272
                     /locus_tag="U2I75_000921"
     CDS             35048..35272
                     /locus_tag="U2I75_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN1696072.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            35414..37165
                     /locus_tag="U2I75_000922"
     CDS             35414..37165
                     /locus_tag="U2I75_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1696073.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            37165..38949
                     /locus_tag="U2I75_000923"
     CDS             37165..38949
                     /locus_tag="U2I75_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1696074.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            39284..40135
                     /locus_tag="U2I75_000924"
     CDS             39284..40135
                     /locus_tag="U2I75_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1696075.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            40232..42118
                     /locus_tag="U2I75_000925"
     CDS             40232..42118
                     /locus_tag="U2I75_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1696076.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(42159..43061)
                     /locus_tag="U2I75_000926"
     CDS             complement(42159..43061)
                     /locus_tag="U2I75_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1696077.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            43291..44694
                     /locus_tag="U2I75_000927"
     CDS             43291..44694
                     /locus_tag="U2I75_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1696078.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            44691..46127
                     /locus_tag="U2I75_000928"
     CDS             44691..46127
                     /locus_tag="U2I75_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1696079.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     ENEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            46272..47198
                     /gene="trxB"
                     /locus_tag="U2I75_000929"
     CDS             46272..47198
                     /gene="trxB"
                     /locus_tag="U2I75_000929"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN1696080.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            47412..49139
                     /locus_tag="U2I75_000930"
     CDS             47412..49139
                     /locus_tag="U2I75_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN1696081.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            49155..49505
                     /locus_tag="U2I75_000931"
     CDS             49155..49505
                     /locus_tag="U2I75_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN1696082.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(49599..50459)
                     /locus_tag="U2I75_000932"
     CDS             complement(49599..50459)
                     /locus_tag="U2I75_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN1696083.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            50551..50979
                     /locus_tag="U2I75_000933"
     CDS             50551..50979
                     /locus_tag="U2I75_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1696084.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            51141..51317
                     /locus_tag="U2I75_000934"
     CDS             51141..51317
                     /locus_tag="U2I75_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN1696085.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            51344..51790
                     /locus_tag="U2I75_000935"
     CDS             51344..51790
                     /locus_tag="U2I75_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN1696086.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        16904 a         9159 c        11152 g        14586 t
ORIGIN      
        1 ttttttgagt ttcctcctct tttagatgaa atatgaataa attatgaagt tcttacttta
       61 atacgcttaa gatatttcaa ttatctataa ctaatgataa aatgaaaagt aaagtctaca
      121 tagtctggat aatacacaga aggaaaagga gattacacaa cgcatggcca atttgattcg
      181 ttcacttatc gaaaacgata agaaagaact aaaacgttta gatagcattg caaaaaaagt
      241 tgaagcatac gctgaccaaa tggcagcatt aactgatgaa caattacagg caaaaactgc
      301 tgaattcaag ggcagatacc aaaaaggaga aacattagat caattacttc cggaagcttt
      361 tgcagtcgtc cgagaagcag caaaacgtgt attggggtta tatccatacc acgtacaatt
      421 aatgggtggg atcgtgcttc acgatgggaa tatccctgaa atgcgtactg gtgaaggtaa
      481 aaccttgact gcaacgatgc cggtgtatct gaatgcgttg actggcgaag gggtccacgt
      541 agttaccgtc aatgaatatt tagcaacccg tgactcgacg gaaatgggcg agctttataa
      601 tttcttagga ttgacagttg ggttgaatat caactcaaaa tcttcggatg aaaaacgcga
      661 ggcttataat tgcgatatca cttatagcac gaataatgaa ttaggatttg actatttgag
      721 agacaacatg gtcgtttacc gtcatcaaat ggtacaacgt ccgctgaatt atgccattgt
      781 cgatgaagtt gactcgatct tgattgatga agcgagaacg ccgctgatca tttcagggca
      841 agcagaaaaa tcaacagctt tatatacaag agcagacaac ttcgtgaaac gtttgaaaga
      901 agaagaagat tataaaatcg atatacaatc aaaaacaatc agtttaactg aagcagggat
      961 cgaaaaagct gaagaaacat tcggtttgga caacttatat gacattgaaa atactgcatt
     1021 gacccatcac ttggatcaag cattgagagc caactacatc atgcttttag acatcgatta
     1081 tgttgttcaa gatggaaaag tattgattgt tgaccaattt acgggtcgga tcatggatgg
     1141 acgccgttat tcagatggtc ttcatcaagc gatcgaagct aaagaaggcg tagagatcga
     1201 agacgaaacg aaaacgatgg cgacgatcac tttccagaac tatttccgta tgtacaagaa
     1261 acttgcgggg atgactggta cagctaagac agaagaagaa gagttcagag agatttataa
     1321 tattcaagta taccagattc cgacaaaccg tccggtcatc cgtgacgatc gaccagattt
     1381 gctttaccca acattacaaa gcaagttcca tgctgtcgta caagatatca aagatcgcta
     1441 ccgcaaagga cagcctgtct tagtcggaac tgttgcagtt gaaacatctg agttattatc
     1501 agatatgctg aatcaagaac gtattcctca tgaagtactg aatgcgaaaa atcactttaa
     1561 agaagcagaa atcatcatga atgcgggtca aaaaggtgca gtgacgattg ctaccaacat
     1621 ggctggtcgg ggaacagata tcaagctagg gcttggcgta agagaacttg gtggcctagc
     1681 agttatcggg acagaacgac atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc
     1741 aggacgtcaa ggagaccctg gtatgtcaca attctacttg tctttagaag atgatttgat
     1801 gaaacgattc ggttcagaac gaatcaaagc tttcttagat cgaatgaaga tcggtgacga
     1861 agatgctgtg atccaaagca agatgctgac gaaacaagta gaagctgcac aaaaacgcgt
     1921 agaaggaaac aactacgaca cacgtaagaa cgtcttgcaa tacgatgacg tgatgcgtga
     1981 gcaacgtgaa gtaatctata aacagcgtca agaagtgatc atggaagaaa aaagcttatc
     2041 taaaccactg atgaatatgg tcaaacggac aatcagtcgt gtggtagatg cgcatacgca
     2101 attagaaaaa gaaaattgga atttggaaag tatcgtcgat tttgctggca gcacgcttgt
     2161 ccatgaagac agtatttccc ttacagatat cgaaggaaaa acctctcaag aaatcaaaga
     2221 atatctaaac gaacgagcac aagaagtatt caataccaaa gcagctcaat tgaacggacc
     2281 agagcaactg cttgaatttg aaaaagtcgt gatcttacgt gtcgtggact ctaaatggac
     2341 agatcatatt gatgcaatgg accaattacg tcagtcaatc ggcttgcgtg cctatggtca
     2401 aaacaatcca ttagtcgaat accaaacaga aggttataag atgttcgaag atatggtagg
     2461 tgccattgaa tatgaagtca ctcgtatctt catgaaagct gagatccgtc aaaacgtaca
     2521 acgtgagcaa gtttctcaag gaacagcttc tcactcagaa gatggagatg ccaaagaaaa
     2581 cagtaatact agcgcgaaaa aacaaccaat ccatgtgaac aaggttggta gaaatgatcc
     2641 ttgtccttgt ggaagcggga aaaaatataa gaactgtcac ggaaaagatg caaactaatt
     2701 tctgacagca ggattggtga gacaaattct tttttaaaga atttgtctca ccttctttct
     2761 gttatttgac gattctgtta gactagatat aataaaaaaa gtatagtgaa agaaggaaaa
     2821 aaacatggaa atatccgaaa ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag
     2881 cttcaggagg tctctttgac ttagaccagc tggaagaaga cattgcggaa gcagaagcaa
     2941 aaatggctga acctggtttt tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa
     3001 acaatgcaaa taaagaaact tatgatcaat tccattctct tgctgaagaa ctggaagaaa
     3061 tcgaattgat ggtggagatg ttgcaggaag aacctgatgc agtaatgcaa gaagaagcag
     3121 aagcacgtat caatgcgcta gatgaaaaaa tgaatacgta tgaattatct atgttgttag
     3181 atggtcctta tgatcgaaac aacgccatcg tcgaattgca tccaggggct ggcggaacgg
     3241 agtcccaaga ctggggcagc atgcttttac gcatgtatac acgctgggca gagcagcatg
     3301 ggtatcaagt agagacacta gattatcaag caggcgatga agcaggaatc aagagtgtga
     3361 ccctactgat caaaggacac aacgcatttg gctatctgaa atcagaaaaa ggtgttcatc
     3421 gccttgtacg tatctcgcct tttgactcag caaaacgtcg tcatacttct ttttgttcgg
     3481 tagatgtgat gccagagctt gatgaaacga tcgatataga aatcaatcca gatgatttga
     3541 agatcgacac atatcgggcg agtggtgcag gtggtcagca catcaataag acagaatccg
     3601 ctgtacgaat cactcacttg cctacaggaa cagtagtagc gagtcaggca caacgttcac
     3661 aattgaaaaa ccgggaacaa gcgatgggga tgctgaaagc taagctttac caattagaag
     3721 tggaaaagaa agaacaagaa gcagctgcac tgagaggaga acaaaaagaa atcggctggg
     3781 gatcacagat ccgttcttat gttttccatc catattctat ggtaaaagac catcggacaa
     3841 acgtagagac aggaaatgtc caagctgtta tggacggaga tcttgattta tttattgacg
     3901 gctatttgaa aatgcatctg caataagagc cctttgttaa attgaaataa aattgtaaga
     3961 ttttgcaagc aagttgaaac attgccatgc tataatagct aaagactgaa aaagatatgg
     4021 agagaacgcc atgattgaaa tgaaggatgt aatgaagaag tactcaaacg gtaccactgc
     4081 tatccgcaat ctctcgattg tcatcgaaca aggagagttt gtgtatgttg ttggtccgtc
     4141 tggagctggt aaatctacat tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg
     4201 aactttgaac gtcgcaggtc atgatctaat agccatcaaa aaccgcgaag ttccttatct
     4261 acgcagagaa atcggcgtgg tgttccaaga ctataaatta ttaccaagaa aaacagtata
     4321 tgaaaatgtt gcgtacgcca tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg
     4381 agtaatggaa gtgctggatc ttgttggttt gaaacacaaa gtccgagtct ttcctagcga
     4441 attatctggt ggggaacaac aacgtgtttc tattgcccga gcaatcgtaa atacacctaa
     4501 agtacttatc gcggatgaac cgacagggaa tctagatccg gaaaactcat gggaaatcat
     4561 gaaactatta gatcggatca atgcacaagg aacaactgtc gtgatggcta cacataacag
     4621 tacgatcgta aatacgattc gacaccgagt aattgctatc gaaaacggcc gcattattcg
     4681 agaccaagcg gaaggggaat atggatacga tgattagaac ctttttccgc catttattag
     4741 aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt agcttcggtc agtgcggtaa
     4801 cgatcacgtt gactttagtt ggtgtgttca tggcggtcat catgaacgca accaaactag
     4861 cacaagatat agaaggaaac gtggatgtat ctgtgttcgt agacattggt acaaaagacg
     4921 aagagatgaa aaaattagaa aaagaattaa atgacttaga tcatgtcaaa aaagtcgaat
     4981 tttcaagcaa agaacaggaa ttgaagaaaa tccaaaacga aatgggcgat tcttggaaat
     5041 tgtttgaagg ggataataac ccgttatatg atgtatacgt agtaagtgct accgatcctt
     5101 cttatacaaa acaagtagca gaaaaagcgg cagatttgaa gaatgtttcc cgttctgatt
     5161 acggtggggc ttcctcagat cggattttcc aaattgctgg tgcagttcga acatggggat
     5221 tagttgcggc ggcattgctg ctgtttgtag caatgtttct catttctaac acgattcgga
     5281 tcacgatttt atctagacag cgtgaaattc aaatcatgcg tttagtaggg gcaaaaaatg
     5341 gatatatccg ctggccattt ttccttgaag gtggttggat cggcttaatc ggtgccattc
     5401 ttccgatact gatcattaca tttggttacg catggtttta ccaattgatc aatccgtcat
     5461 tattacgatc tcattattca ttgatccatc cagaaaatat cgtttggaaa atcaatcttt
     5521 tgatggtcgg tattggtgtg atcattggtt cactcggctc gatcatctct atgagacgtt
     5581 tcttgaaatt ctagtgtcac aagataaata taatcagaaa gttggacgat ttcgttccag
     5641 ctttcttttt ttatcagcta ggtggagtaa atggggcggc ttttttctct ttactttaaa
     5701 aaacaacttg ggtattctta agttggtaga aaaagaggtg aagccgtgaa gattatcgtt
     5761 gacaattttt ttacgatcct tttgattatg aatattttgc tttcctttat tatcgttttt
     5821 cgagaaagaa aggaaactgc gcaaacatgg gcatggctgc tcgttttgat gttcattcca
     5881 gttgtaggat ttattttata tattttttta ggaagaggga tttccaaaga gaaaatcttt
     5941 gatttgaaga tccaagggaa aatcggtaag aatctagaaa tcgaagaaga taaacaagca
     6001 ttgatgcgag ggctttatcc tcatccgcct actggacaag tagatgtgaa acaattgatc
     6061 tatatgctta ctgtatttga aagtacgctt tatacgacta aaaatgaaat cactttattt
     6121 acagatggtc gtgaaaaatt cgatgcgctg atcgaggata tcaaacaggc agaagaccat
     6181 atccatttcc aatattatat ttaccgcagt gatacattag gtgaagaagt tcgagaggca
     6241 ttgacagagg cagcaagacg cggagtaaaa gtcagagtcc ttcttgatgc atggggttcc
     6301 acacaagttt cttctagttt ttttcagaat ttgaaaaaag caggtggaga agtcgcattt
     6361 ttctttcctc tattcgttcc gtatatcaat cctcgaatca attatcgcaa tcatcgtaaa
     6421 atcgtagtga ttgatgggaa aatcggatat acaggtgggt tcaatgttgg aaacgaatat
     6481 ctaggattgg taaagaaatt tggttattgg cgagacaatc atctgcgtat ctatggagaa
     6541 gcggtttaca ccttgcaaaa ccgttttttg atggactgga attctcagca tacaaaagaa
     6601 gtaggatatt caccggaatt tttcccgacc attcattcta caggtgatat ggctgttcaa
     6661 atcgtgacaa gcggaccaga taccgaacat gagcaaatca aaatgacata tttgaaaatg
     6721 atttcgttag ccaaacggga gatattgatc caaacgccgt attacatacc ggatggatcg
     6781 attcatgagg cattgaagtt ggctcttctc tcaggcgtaa aagtccatat ccaaatacct
     6841 aacaaaccag atcatcttct cgtttactgg gcaacgtatt ctttcgctgc agagctgatc
     6901 gagtatgggg caagaatcga aacatatgaa aatggattta tccatgcaaa gacgatgatt
     6961 atcgatggcg gcatcgtttc cgttggttca gctaatatcg acgttcgctc atttagattg
     7021 gattttgaag tcaatacact gatctatgat gaacgaatgg cttctcgcgt aagaaaagca
     7081 ttctttgaag attcaaagat ttcgacgcat ctcacaaaag aagtgtatga aaaccgaggg
     7141 atgatgatca agatgaaaga aggattagca agactgattt cgccgctcct ttaaaataca
     7201 aaaaggacag gagagccatc aatagatgga aatctcgcgt ccttttttct cggggccatt
     7261 tctgagagta cgtcagaaat ggagaaagcg tctcgcatga gcgaaattaa ctttggggta
     7321 tctcttgttt tttggggaaa gcaagaaata agctaatata tgctattaaa agagacacat
     7381 ttcaaagcca gtatagcata gaaactttta atgagatagc tttttttgca attatgagaa
     7441 tttattttgt tatttaatga ttttgattcg ttttcatcaa gtaatgaaaa tgatagaata
     7501 aacatacttt aggattaaag gagatttcta atgaaaaaac taaaattatt cttaccaata
     7561 ataggattag ctattctttt atcaggctgt tcaaagtgga ttgatcgagg agaatccatt
     7621 actgcagttg gctcttctgc cttacagcct ttagtagaaa cggttgctga aacctaccag
     7681 gcagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac tgggttgtct
     7741 caagtacagt caggagcagt cgatatcgga aattctgatt tgttcgcgga agaaaaatca
     7801 gggattgatg cttcggcatt agtcgatcat cgagtagcag ttgttggaat cactcctatt
     7861 gtgaacaagg atattggcgt aaaagatatc agtatggaaa acctgaaaaa attatttacg
     7921 ggcgagatca aaaactggag tgaagtcggt gggaaagatc agccagtagt tatattgaac
     7981 cgtgcatcag gaagcgggac ccgtagtacg tttgaaaaat gggtattgga cggagaaaca
     8041 gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccaaatcgt tgcggacaca
     8101 cccggagcaa tcagctatgt tgctttttct tacgtttccg atgatgtgga tacgttgaac
     8161 gttaacggtg tggaaccaac agatgaaaat gtgacaacga acaaatggac gatttgggca
     8221 tatgagcata tgtacacaag agaccatcca aaaaaactga caaaagaatt tttagactat
     8281 atcttatctg atgaagtaca agataatatt gtcggagagc taggctatat ccctgtttca
     8341 catatgaaag ttgaacgtga ttggcaagga aacattgtta aagagtaatc tttacacaaa
     8401 atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt tcaatttgta
     8461 cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa
     8521 gtattattaa caaaatcaaa acgttcaaaa atggaacagc gcggaaaatt cattagtttt
     8581 ttgtgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt cgctagtaaa
     8641 ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt tggtacagat
     8701 tggaatccaa gccaaatagg agcaaatggg aaacccatgg taggggcatt gcctatgatc
     8761 acaggttcat ttattgtgac ctttttatct gctattgttg ccacaccatt tgctattgga
     8821 gcagcagtgt tcatgacgga gatttctcct aaaaacggaa cgaagatctt gcaaccggtc
     8881 atcgagctac ttgtaggtat tccgtctgtc gtttacggat ttatcggttt atctgttatc
     8941 gtgccttttg tcagaagtat ctttggcggg acagggttcg gtatcttagc tggtacattt
     9001 gtgttattcg ttatgatctt accgactgtc acaacgatga ctgtggatgc attaaaggca
     9061 gtgccgcgac actatcggga agcttcatta gcgctaggtg ccacaagatg gcaaacaatc
     9121 tacaaagtgg tgctgcgagc agcagtgccc ggaattttaa cagcagtcgt ttttggtatg
     9181 gctcgtgcgt ttggcgaagc cttagctatc caaatggtta ttggtaatgc ggcattaatg
     9241 ccaacaggac ttactactcc tgcttctaca ttgacgtcca tcttgacaat gggaatcgga
     9301 aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct gatcttgcta
     9361 ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa
     9421 taatgaacgc aaaacgctca gataaaattg ctacaggtgt tctatatgtg gtttcaggga
     9481 tcatcgtatt gatcttagca gctttattac tttatatctt agttcgagga ataccgcaca
     9541 tttcatggtc attcttgact gaaccttcca aagcctacca agaaggcggt gggatcggga
     9601 ttcaattatt taactcgatt tatttgcttt tgattaccat gatcatcagt tttcctatct
     9661 ctttaggtgc gggtatctac ctttcggaat atgccggaaa aaactggctg acagacgtga
     9721 tccgtacgtc tatcgaaatc ttgagctctt tgccttctgt tgttgtcggt ttattcggtt
     9781 tcttggtttt cgttattcaa ttccagtatg gtttctcaat catttctggt gcattagctt
     9841 tgaccgtatt caacttgcct cttttgactc gaacggtcga ggaatcttta caagcggtcc
     9901 attacacaca acgagaagct ggattggcgt taggtttgtc acgctgggaa acagtgataa
     9961 aagtggttgt tccagaagcg acaccaggaa ttttgactgg tgtgatccta agttcaggaa
    10021 gaatttttgg tgaagctgcc gcattgattt atacagctgg tcaaagcgcg ccagcattag
    10081 attttacgaa ctggaatcca ttaagtgttt ctagtccgat cagcattttc cgccaagctg
    10141 aaacactggc tgtccatatt tggaaaatca acacagaagg tacgatgcct gatggtgttt
    10201 ctgtttcggc tggcgcttct gccgtgttga tcattgctgt tttactgttc aacttcggcg
    10261 ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga gaagaagtaa
    10321 aatgaaaaaa tataattgga atgaaacaaa tgtcattacc atgccaaaag ataaggaagt
    10381 tgcattatat acgaatgatc tgcatgtttg gtatggcgac aatgaagcaa tcaagggagt
    10441 ggatctagaa ttcgaaaaaa ataagatcac tgcattgatt ggtccctctg gctgcggaaa
    10501 atccacgtat cttcgttctt tgaatcgtat gaatgatggg attgctaata cgaaaattac
    10561 tggaaagatt atgtataaag acgtggatat caatgcgcct caagtggatg tttatgaaat
    10621 gcgtaaacgg atcggcatgg tgttccaacg ccccaaccct tttagcaaat cgatttatga
    10681 caatataact tttgctttga aacagcatgg tgaaaaaaac aaaaagaaat tagatgaaat
    10741 cgttgaaaca agcttgaaac aagcagcttt gtgggatcaa gtaaaagata gcttggataa
    10801 aagtgccttg gctttatctg gcggacaaca gcaacgtttg tgtatcgctc gtgcaatcgc
    10861 gatgaaaccc gatatcttgc tgttagacga gccggcaagt gccttggatc ctatttcgac
    10921 aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tacacgatca ttatcgtgac
    10981 gcataatatg caacaagcag ctcgtatcag cgactatact gcctttttct atctaggaaa
    11041 agtaatggaa tatgacaaaa caaacaagat atttaccaga ccaaaaattc aggcaaccga
    11101 agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa aagaaattat
    11161 ctcatccaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaagg gaattgatct
    11221 aagtttcaat caaggtgaaa tcacagcgat gatcggtcct tctggttgcg ggaaatctac
    11281 ctatctacgt tcgttgaatc gaatgaacga tctgattcca aatgtaacga tcaccggaag
    11341 tgttttatat aaagacaaag acatctatgg tccgaaaaca gatgttgttg aactaagaaa
    11401 agaaatcggc atggtgttcc aacagccaaa tccatttcct ttttctattt atgaaaatgt
    11461 catctatgga ttgaagttaa aaggtgaaaa agataaaaaa gtccttgatc aagtagtgga
    11521 agaaagtctg aaggcagcgt ccgtttggga ggatgtgaaa gataaactgc ataaaagtgc
    11581 cttgtcttta tccggtgggc agcagcagcg tgtatgtatc gcacgtgttt tggcagtgaa
    11641 tcccgaaatc atcttattgg acgaaccaac aagtgcactt gaccctgttt ctactgggaa
    11701 aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg tgacccataa
    11761 tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg gtcacttgat
    11821 cgaatacaac gatacgaaga agatattcat gaatccagaa aaacaagaga cagaagatta
    11881 tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga
    11941 attgctgaat ctccataatc agttctacga aatggggatg atggtgagca gcgctgtgca
    12001 caagtcagtt cgtgcataca tcaaacacga taaaatctta gctcaagaag ttatcgaaaa
    12061 cgatataaat atcaataata tggaaacgcg tttggagaag aaaagttttg aaatgattgc
    12121 attacaacag ccagttacaa ctgacttgcg tatgattatc acggtcatga aagctagctc
    12181 tgatttagaa cgtatggccg atcatgcggt gtccgttgct aaatccacaa tccgcgtaaa
    12241 aggacagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg attacgtgaa
    12301 aaaaatggtg gacaacgtcc tagttgcgta tgtcaaaaca gacgaaaaag atgcgcgtac
    12361 aatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata atgcaaccat
    12421 caaaaacatg caagaaaatc ctgaaacagt catcagtggc acagactatt tgaatgttgc
    12481 cagctatcta gaacgaatcg gagactatgt gacaaacatc tgtgaatgga tcgtctactt
    12541 ggctacaggg aaaatcactg aattaaatac gaaccacaat gagattttct aaactgaagt
    12601 tgtgattctg ctgtttattt aggaaaagag gttgtgaaat cagcgttttg tcctgagtat
    12661 tagagaaaca aacggtaaaa tagttcctca tattttgccg ttttttgagc taataccgaa
    12721 ggtcagctga ttgaacaccg tttattctgg aaaagggttt gtgacaaaac atgtcacaaa
    12781 cccttttcat tattccaata gaaaggataa agaatgcaaa aaaagattag catcatcatc
    12841 actctatttt ttatttgtat acttgcttca tggtttattc agcgtcaagc tgctctatat
    12901 gacgtacctg tcatcgagat cgaaagagtg agaatagaag aggatcaaca attgattaca
    12961 ggaagattga tgaatacgga ccaaaaagga gaagtgattt ctttaacggc tccttatcaa
    13021 aaaaatcaaa tcgaaacagc aaatataaca acaagacaaa tctatcttgt ccaaatgcat
    13081 ggagatgaat ggcaactagt agcaaaaaaa aatgatggct ggcttttttt ctttacaagt
    13141 gtcttcattg gcaccatgtt gcttattggt ggaaaacagg gcgtatttgc tttaggaagt
    13201 ctcatcatca acatcgtttt acttttactt ttgttgctcc tctttaccaa aacagaaggt
    13261 atttctctct taaatatagc aatcttattt gtcgttatag ggataatcgt tagtatattg
    13321 gcgttagacg gatggaatag aagcagtata atcaaaattt cggctgcagt tacaagtgtt
    13381 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagatca aggactgcgc
    13441 tatgaagaac tgaattatct gacacgaccg tatcgttctg tttttctttc cagcgtgctg
    13501 attgggacag ctggagggac actcgatacc gtgatcacag tcattgcgac attagaagaa
    13561 ttgaccaagc aacaaccgaa aatatcagcg aaacagttat ggctttcagg aaggcaagtg
    13621 ggccgagatg tgattggttc gatggctaat atccttttat tcgtctatat cagcggagcg
    13681 attccatcgc ttctgttata tttaggaaat caatggtctt tcaaagaaac ggtagaacga
    13741 catttatcat tagaactttt acgcgtcatt gctggcagct taggaatagt attatcaatc
    13801 cccatcgcgg tcctcttttt tctcgctgta aggaggttaa aaaaatgaat gtacttatta
    13861 tacttgttgg gatttttgca atcagtgtgc tatttgtcgg tggtacgcaa ggtatgtata
    13921 ttcttttagg attattcatt aatttaggaa tctttttcct attattattc ggctatcatc
    13981 agaagtggcc gatattggta ttgtcgatta tagggttcct attgatcgct gtggttatct
    14041 tattttttat caatggttat aatctgaaaa tgcgagccgc ttttgccagt atactgatct
    14101 ttttgttttg tttcctacta ttgattccta tcactgattt tttagcgatc caaggcttta
    14161 cttcgataga actagaagaa ctatctggct tagataagac gttagcaatt gattttagac
    14221 tacttacgag gtcgttgctt ctgatcagtc ttagcggagc tgttttagat gcgagtgtag
    14281 cgatttctag tggaacattc gaagtatatc aggcaaatcc gcaaatgtct tttaaccaat
    14341 tgcgccatgc tagctttgcc attgctaaaa aaatattggc atcaacagtg atgacgcttc
    14401 tttttgcgtt tatggggagt tctttagcgt tgattctttg gtttattgac ttagacattt
    14461 cttttcagca aataatcaat gaaaaatcat ttgtcttgga atatacgatg gctattctta
    14521 ccacactggc cgcactactt gtcctgccct taacttgcgt gactacagca tatctcttta
    14581 caaaaaagaa agaacatctc aagatggagt aaaagttaca acttaagtcc tatgacattt
    14641 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
    14701 aggcaatttc catgaaagaa agagaacgta ttttagactt agtgaaaaaa ggaatccttt
    14761 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaaacaaa
    14821 tcaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
    14881 tgatcgatga acttgaaaac agtgaagaaa aaatttctga gccagttgat ccaaaagaaa
    14941 aagaaagaca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
    15001 ataaaacttc ggcgcgtcta gacgaagtaa atgctcaatt cgctgacaat aaggcagcac
    15061 gtaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
    15121 cagaagaaga attagcacag cgtcaaacgt tagaagcaga aatcaaagag ttagaagcaa
    15181 ccggcgatac actattagaa gaacaattga agctggaagc agaattaaaa gatatccgca
    15241 agaatcaatg gtctgagaaa aaagaaacat ttacggataa gtttgaattg ccagatgatt
    15301 ggaaagatca agcgacagat acgctgaatc aagtaggcga aaaaatgtca gaagctggat
    15361 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtaaatgata
    15421 acatggaatg gaaagatgtc agcttacgcg tccctggaat tgcgacaacg aaatttgaac
    15481 acgaatttta ttatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
    15541 acgtgacgtt aaaaacgtgg gacagtgatg atgtgaaagt ggaagccaag atcaaacttt
    15601 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag atcgaggtca
    15661 atgaagatca tatctccttc caaattccaa ataagcgtgt gcgtgcagac cttgttttct
    15721 acttgcctaa acgagtatac gaccatgcag ctatcaaact tctaaatggc aacatcatga
    15781 ttgagacatt ggaagctaaa gatatttata caaaatctac gaatgggaac atcatcgtga
    15841 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
    15901 acgggaacat cttagattct attatagaaa cagtaaacgg tacagtgaca tttggtgcca
    15961 caccagaaaa cctttctgtg tctttagtaa atggtgatgt acgtcttacg attaaagaag
    16021 ataatttgaa aaaagtagaa gcaagttcag tgaacgggaa tgtcaaagtt gctttaccag
    16081 atagcatcgg tttggaagga cacgctaaaa caagtcttgg aagcattaac agccgtctgt
    16141 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
    16201 gtgtttcaga tgatgagatc gcacaagtgc agctttcaac gacaacagga agtatctatt
    16261 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
    16321 gcgatttttt aaaatagctt cccctatttt aaaaaaatgt gccttattgc cgataggcta
    16381 tctcttgaac accgattaag aagaaggttg tcacaacgat tttcttcatt taaaatagta
    16441 gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ccaataaaaa tgttgttttg
    16501 acaggtactt tagcagggat tggggaatat atcgggattg atcccactgt tatccgtgtg
    16561 ctttatgtat tcttaagctt agttgctttt ggaagtccaa tcattttata cattttatta
    16621 gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa caatgcatat
    16681 tatgaaaaaa acaattatcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16741 gatgacgaat ggagtgactt ttaatgtcgt actttcagcg tattttggtt aatacgttaa
    16801 cattcatatc gttggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    16861 ccgtccttgc cagcttcgta ttatctgtgt tgaacatgtt tgtcaaaccg attttgacga
    16921 tcctgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    16981 cgattcttaa aatgacttcc ttttttgtag gagcagaaca ttttagtttt tcttcatttg
    17041 gtgcagcgtt attcgtagct atcttgatgt cacttgtcaa tgtaattgtt agtgaacata
    17101 acagcagaaa ataaaaaaag ctgtgccgag atctcggcgc agcttttttg tttagaataa
    17161 agaatgcgtt ttctattaca ttggtaatta aataaaaacg ttttttttaa ttggtagtac
    17221 ttttggctag acaatgataa aatgatagag aacactctaa tagaaatgag gtagcatgaa
    17281 tgtcagaagt tgtaaaagtt agccaattag taaaaaaatt atctttggaa atcgtcacag
    17341 gcgacgagaa aagtttagac cgcgtcatcg tgactgggga catctcacgc ccaggactag
    17401 agttgaccgg ttattttaat tattattccc ataatcgttt acagctattt ggcagtaaag
    17461 aaatcagttt tgcggaacgg atgatcccag aagagcgttt gatcattatg agaagattat
    17521 gtagtgaaga tatgcccgca tttattattt caagaggatt gccggctccg gaagaaatga
    17581 tccaagcagc caatgaaaat gggatagctg tgctgcgttc gcctatctca acgtcccggc
    17641 tgctaggtga attatctagt tatttagatg gacgcttagc ccctcgaaca agtgtccacg
    17701 gtgtgttagt cgatgtctac ggattaggtg tactaattca aggggacagt gggataggga
    17761 aaagtgaaac ggcgttggaa ctgattaagc gcggacatcg attgatcgca gacgatcgag
    17821 tagatgtcta tcagcaagat gaactgacgg taatcggaga acctcctaaa atcctggaac
    17881 atctgattga gattcgaggg atcggtatca tagatgtaat gaatcttttc ggtgcgagtg
    17941 cggtacgagg ctctatgcaa gtccagttag ctgtttatct agaagcatgg gcgaaagata
    18001 agaaatacga ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa gtgggcattc
    18061 cacaaataaa aatccctgtc aaaacaggac gaaacgttgc aatcatcatt gaagtagccg
    18121 caatgaattt ccgcgcacgt acaatgggct ttgacgcaac gaaaacattt gaagaaagat
    18181 taagtcgctt gattgaagaa aactcaggca atgactagca aggagtgaca atatgttagc
    18241 acaaatcaat cgagttgctt ttgagcttgt aggaattcct atctactggt atgcattgat
    18301 tatcgtatca ggaatcatta ttgccatgtg gctcagcagc agagaagctg ttcgggtagg
    18361 attaaaagca gatgacgtga cagattttat gttgtgggga ttgccgattt ccattattgg
    18421 tgcccgcttg tattatgttt tattcgatct tccccaatat atagctgatc caatccagat
    18481 attcaatatc cgttcaggag gattagcgat ttacggggga ttgattgcag gtgcaatcac
    18541 actgtacttt tttaccaaat atcattttat ttctttgtgg acctttttag atatcgctgc
    18601 accaagcgta ttattggcgc aggcaatcgg acgatgggga aatttcatga accacgaagc
    18661 gtatggtcca gacacgacac gaagtttctt agaaagtctg catttaccgc aattcatcat
    18721 tgataacatg tatatcgaag gaatctatcg acaaccaacc tttctttatg aatcactttg
    18781 gagtttgatt ggatttattc tgcttttggt tttgcggaag aaaccacatc ttttgaaaaa
    18841 aggagaagtc cttttatgtt atctaatgtg gtatagtttc ggacgtttct ttatcgaagg
    18901 gttacgcaca gacagccttt actttttagg tgtgatccgc atttctcaat tattgtctgc
    18961 tgttttattt gtcggcacat tgattctgtt tatttggcgt agaaaaaaag gaagtttacc
    19021 ggattatgac cgctcattag gaaaaaatca acaaattatt tgattttcag agcgttaaaa
    19081 atgaaaaaaa tttcaggagt ggtaaaatga attatagagt agcggtttta ggaccaggct
    19141 cttgggggac tgctttagca caagtcctcg ctgaaaacgg acatgacgtt cggatttggg
    19201 gacatcgtgc ttcacaagta gaagaaatca acacccaaca caccaatcaa cgttatttac
    19261 cagatcatca tctacccgat tccatccgag cattcaccga tatggaagat gcagtaaaag
    19321 atgcagatgc ggtactattc gttatcccga cgaaagcgat acgttctgtt gcaaaagaac
    19381 tagttgccaa attgaatacg aagccagtga tcatccacgc aagtaaggga ttagaacagg
    19441 gaacgcataa aagaatctct gaaatcttgt tagaggaaat cccgacagaa aaaagaaaag
    19501 ctgttgctgt tctttctggg ccaagccatg cggaagaagt agcaaagcat gacattacga
    19561 cgatcacagc ggcaagcaca aatgtaaaag cagcaaaata tgtacaagag ctgttcatga
    19621 accattattt tcgaatctat accaaccctg atgtgattgg tgtagagatg ggagcagctt
    19681 tgaaaaatat tattgccatt ggagctggtg ctattcacgg cttagggttt ggcgataatg
    19741 caaaagcggc aatcatgacg cgcggattag ctgagatcag ccgtttaggt gtagcaatgg
    19801 gtgccaaccc gctgaccttt attggcttaa gcggagtagg tgatctagtc gttacttgca
    19861 caagtgttca ttccagaaac tggcgcgcag gcaacttgtt aggacaaggt cagcctctag
    19921 aagaggtgct agccaatatg ggaatggttg ttgaaggtgt gaatactact caagcagcga
    19981 tggaactagc ccaaatgatg gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc
    20041 tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat catgttacgt gatgggaaaa
    20101 aagaaaatga attctcatta acaatttttt gaaggagcga atgtatatga aagtaaaaaa
    20161 agcagttatt ccagcagcag gattaggaac aagattttta ccagcaacaa aggcgatggc
    20221 aaaggaaatg ttgccaatcg tggacaaacc aacgatccaa ttcatcgttg aagaagcgtt
    20281 gaaatcagga atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga
    20341 tcactttgat tcaaatatcg aattagaaac aaacctaagt gaaaaaggaa aaaccgaatt
    20401 attgaaatta gtagaagaaa cgacagatgt caatttgcat ttcatccgtc aatctcatcc
    20461 aaaggggtta ggacacgccg ttcttcaagc aagagccttt attggaaatg aaccgtttgt
    20521 cgttatgcta ggcgatgata tcatggaaga cgaagtacca ttgacacagc aattgatcga
    20581 tgactatgag cgtacacacg cttctacgat tgccgttatg caagttcctc acgatgaaac
    20641 ttcaaaatac ggaatcatca acccagggga agtactggaa gatgggttat acaatgtcaa
    20701 aaactttgtt gaaaaaccag atccaagcaa agcaccaagt gatttagcta ttattgggcg
    20761 ttacttattg actccagaaa tctttgatgt attatcagaa caacaaccag gcgctggaaa
    20821 tgagatccaa ctaactgacg caatcgatac gttgaataaa acacaacgcg tatttgcccg
    20881 taaattcaca ggtaaacgtt atgacgttgg tgacaaattt ggttttatga aaacaagtat
    20941 tgaatatggt ctgacacatc cagaagtagg ggcacctttg cgtgaataca tcatgtcttt
    21001 aggtaaaaca ttagaagcag aaggaagcaa gatcaatcat aatccaaaag gaataacaga
    21061 aatcaaagaa gaaaagaaaa aataatcttt ttttctaacg agcttcagct gctaattgaa
    21121 taaaaagtag ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc tatccttata
    21181 gaaaagagag acaagaaggg agtttgatca tgtcaggagg agaaattgca ggattgatcg
    21241 cagcaatcgc ctttgcgata cttgtcgttt tcatcgttcg cgtattgttg caagtttcca
    21301 aaactttaga aaaagtagac cagactgtcg cagaagcaaa tacgacaatt gagatcgtca
    21361 cacaagacgt agatctttta tctagacaag tagaaggctt gctagtcaaa agtaatgagc
    21421 tgctcttgga tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg
    21481 atttaagtga aagtgtatcg gaacttaatc attctagtaa aaacatcgtg acgaaagtcg
    21541 gcggaatcgg aaaaacgaca gctaaagcga ctgttgccgg aaaagtcggt ggaacagcga
    21601 tgaaattttt aaaaacaaaa aacatactga aacgacagga ggaaaataag aatggctaaa
    21661 aaaggtggat ttttattagg tgcagtagtt ggaggtacag ccgcagcaat cgccgcatta
    21721 ttgttagcac ccaaatctgg taaagaactt cgggatgaat tggctgctca agcagatgat
    21781 ttgatggaca tggcttcaga ttacacagat atggcaaaag aaaaatcatc tgagttaaca
    21841 ggtattgcaa aagaaaaagc gcaagatctt tctcaacaag caggggatct agcaggcaat
    21901 gttaagcaga aggtaagtga atcagcagag gaagcaggaa gcgaatcaga tgatatcttg
    21961 ttaaacctca aagaacaatc agctgattta tctaatcgtt tcaaaaaaac agcagaagat
    22021 ctgaaaaagc cagcagacga attaggtagt attgctaaag atacttcaga agatatcatg
    22081 atcgatgtaa aagatgctgc agataaagca ggagagacgg tcaatgaagg aatgtcagaa
    22141 gcaaaaccag ttgcagatga cacaaaagaa atgttgaaaa tggcaaaaga agatgcaaaa
    22201 gaagctaaaa aagaaatgaa agatacaatg acagattcga ctaccaatca ctaagattac
    22261 tcatttctag aataaaaaag gtgagagcgg accagctctc acctttttta ttttcattac
    22321 tctaaaacaa gtaattcttt tggtgtagtc gtgaatggca cacaaccatc acttgtcaca
    22381 tacacacaat cttcgatacg tacccctact ttttcaggaa tatagattcc tggttcaaga
    22441 gagaagcaca tcccttcttc aatcactaag tcgtttcctt ctacaagaga agggaattcg
    22501 tggacggttg ttccgattcc gtgacctaaa cggtgattga aatattcgcc gtagccatag
    22561 ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc tgggcgaact
    22621 gcctcttgtg cggcaagctg tgcttcaaga gtgatgttat agattttttc ttggaattca
    22681 gttggttttt gataagcaac tgtacgagta gcatcactgc aatagccatt ccaaataacg
    22741 ccaagatcga ataacactag ttcatttggt gaaaccttcg tgcttcctgg tgtaccatga
    22801 gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg tgaaacacct
    22861 tgttttttta attggtattc gatttctgcg ataattgctt gttcagttac gccttctttt
    22921 actgttttga agccaatttc aaaggcaaca tcggcccatt taccagcttc gatcagtttg
    22981 tctttttctt ctaccgtttt gatcagttgc agacgctgga taatagaagt tagatcggtg
    23041 taagaatctg tacctgtcaa gatatcagag atagcatcga agcgatcgac agtcagagca
    23101 ttcttttcaa tacctacatt accaggttgc ccatagcgtg cgcgaagctc ttttccgatg
    23161 atctcccaag gattttcgct gtctaagtag ccacgtacat cgtacgccca gctgctgttt
    23221 tgagcatctt caacttccag agcaggagtg aaaaggaaag gatcttgttc cagagggata
    23281 aaaagagcta gtacacgctc gtgaggatca ctttcatatc cggaaaagta agcgatgtgg
    23341 ccagggtcgc tgatataagc caattctaat tggtgttgtg ccatccattg acgtagttca
    23401 ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt attcaataat
    23461 tgtaccactg attgattaaa agaccaacgt tcttaaaact gaaaaaagta aaaattttca
    23521 tgaaaacaga gataaaacgg ttgcaattgt tgagattatt tgatatccta ttttagaaat
    23581 gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa tggaaaagca
    23641 aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta ctgtttcacg
    23701 tgtcgtaaat ggcaatccaa acgtcaaacc agcaacaaga aaaaaagtct tggaagttat
    23761 cgatcgcttg gattatcgtc caaatgcagt cgcacgtggc ttggcaagta agaaaacaac
    23821 aacagttggg gtaatcattc ctgatgtcag caatatgttc tttgcctctt tagcacgtgg
    23881 tatcgacgat gtggcaacaa tgtacaaata caatatcatc ttagcaaact cagatggcaa
    23941 cgatcaaaaa gaagtcaatg tattgaataa tttattggct aagcaagtgg acggcgtgat
    24001 ctttatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt caaaaacgcc
    24061 tgttgtacta gctggttcca ttgatccaga tgaacaagta ggtagcgtaa acattgatta
    24121 tgtatcagca acaaaagatg cagtaaataa attagcaaaa agcggcaata aaaaaatcgc
    24181 ttttgtcagc ggtgcgctga ttgatcctat caatggtcaa aaccgtctaa aaggctataa
    24241 agaagcatta agcgaaaaca atctaccata tagcgaagga ttgatttttg aggcacaata
    24301 caactttaaa gatggtctgt ctctagttga ccggatccat aatagcggtg caacagctgt
    24361 ctatgtttca gacgatgaat tagccattgg tatcttggat ggtttattag accgcggcgt
    24421 aaaagtacca gaagaattcg aaatcatcac aagcaacaat tctttactga cagaagtagc
    24481 tcgtcctcgt ttgacaagta tcacacaacc attatacgat ttaggagcgg tatccatgcg
    24541 cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta tcttgcctta
    24601 tggaatcgaa gaaaaaggat caactaaata ataaaagatc aatcatttaa aggttgttcc
    24661 caaagtggtc aacttctgga acaaccttta tacgagaaaa ggggttatga caaaattttg
    24721 tcataacccc tttttttgtg actttaaaaa aaagagagaa tggcggaaac aaaccttacg
    24781 tccatcgtat acgacacaat ctgtgcgtag atgctacatt ttgttcacta ctatcaaaat
    24841 tactttaaaa tgaaaacgtg ataaaaaaga aagtagaaaa gtagaaaagt aactctaaaa
    24901 aatccaaaaa aatccaggct cgattgcgta cttatcttaa aggagaaaca atccatcata
    24961 cataacccga aaggagactt ctatttgcct aagaaaagac gaaagaccat agtcatcatt
    25021 ttgaccattc ttactatagg aatcggagga aaattttaca tggacaaaag agaagcgcaa
    25081 aaacagcaag atttactcgc agtagaaaaa cagtccgtca aagttctgaa aaataccttt
    25141 gcggatatca aagaagtgaa aatcgaacaa tttgagaaaa acgacatgac aggaatctat
    25201 gaagcattga taacgatgac caatacagaa ggaaaagccg tttattttga ttatagcttt
    25261 acaaaagaaa gcaatgagat agatggctat ggtgttgaaa atgaagaagt acaaaaagaa
    25321 gggattacgg ttgatgagat tatagttatc tatacagatg ggagtgagga aaaagtatga
    25381 caattactga taagcagtat aaaggaataa gtaataaagt atattggcta gatccaaaac
    25441 atcctcaata tagtaaaagt tacaaagaag gcgccattaa aaactttgga gaaacaaaat
    25501 tccaaatcct aaaaatcaaa aactccttcg acggcatgca ggcgatggct gtagcgccaa
    25561 tcaagaacgg taaagtggat atttctcaag tagtgattgc ttttgcagga acgaacttaa
    25621 gcgattggaa agatttggaa acggatgcaa gaagtgttat gaatctctat gggaatagac
    25681 caggagcaag tttttctgaa aaagcaacaa caaacaagat tttatccgtt agtggattga
    25741 aaacagcggg gcaaatcaat tcagcgattg catttgctaa tgaagtaaaa aaggaatatg
    25801 gagaagctgc tatttctact accggccatt cattaggtgg attcttagca ttatatgtag
    25861 cagcagagaa acaatggaaa aatgtcggct tcaacgggcc ggacccgtat gatctcttat
    25921 caccagaagc aaaaaaatgg gtggaagaaa atcccggtat gctcaccaat taccgaaatc
    25981 acgccgattt tatcggtaat tttggtggta atggcacagg tgctgaagtt cggatcagta
    26041 tggaaacagc atacttgaat ctgcttgctt cccacagttt aacctcttgg aagtttgatg
    26101 aaaatggaaa gctgattatt cctgaaaatg aaaacaatgt taaagctcga aaacaacaga
    26161 aagaaaacca agcgacccag ttgttttcct atcagttgca agaactgaac aacatacgta
    26221 aaaaactacg attaagtggc ggccggctga cagttaatga acaaatttac ttagatgaca
    26281 cacaagctag gttagtggtg gaaaagttat cggtaaaaat gaaaagcgct atggaagaag
    26341 ttatccgcat caaccaaaga ggaatttttg agctagagaa aaaatggcaa gaaggcttgc
    26401 ggcaagcacg tgttgtcgcc ccttcgttga ctgaacagga aatcatagaa gcattagcta
    26461 gtgtgggcgc cacaaaagag aacttagtcg attctgcgaa agaagtctat ctgctacgaa
    26521 tcaataaggc aagacgtatg ggggaggcct ttgatacatt ggcgaaggag atccaagaca
    26581 aaatcaatga aatcgtggca cgagatcgag aattagcaca gcaattcaat taaaggagaa
    26641 gtagatgaaa ctagaagaat tggaaagaga ttatcgaagt gaacgaagac aattagaagc
    26701 tcaagaagat gaattgatcc actttaagaa tcaaggatta cgattgatgg agcaagagga
    26761 gcagcgactg catcatgtat taaagaactt agctttagat aatgagcctc tgtttcaagc
    26821 aagacgaagt tattcgaatt atcaggagca ttatcaacaa gccctacaag tagaacgacg
    26881 aaaactctat cagcagcaag acgaactaga acgttcctat cgtcaaaaaa tcagacaaat
    26941 aactatggag aaatcatctt atgaatgaat ttttaatgca attactaaaa gaattagaga
    27001 aaaatctaca tcgatcaaat gtacgttcag ttggtatggg gatatctcag caaacttggg
    27061 cagaaaaacg attgacagat caaaaccacc aaatgactgc agaagaagct actcaagaat
    27121 tttctaaccg acatgcacac ttaaaagaat cagtaaaagg gcaatttgca gaaaaactaa
    27181 ccgaattatt ccaaagacaa gaaaagaaat taaaacagtt tcgttagtaa agttatatag
    27241 aaaaattttg tgaaaatggt ttttcacatt tttacataaa tttgtcttaa tatcgtagat
    27301 gtaagctttt gaacaccatt tattcggaaa aagggagctg tgacaagatt tttgtcgcag
    27361 ctccctttaa ttctgattaa tctattagat caatcatctt tatcgtctga ttctttggtc
    27421 tctggattcg ctctagcata agctgaagcg gtgttccagt aatctgtata gttttcatca
    27481 gtaccgccaa cgccgaattt aaaggcgctt ttcttcggac cattttttgc ccaaagtgat
    27541 tccactgttt ttcctggcgt ttggatagat tttttattga cagtaacttt tccagctgtt
    27601 tgtcctgtga acccagatac ttttttcttc atcacatcgg atgataagtc aaatttctca
    27661 tcagtgccga aaatctcagg gtttgcttgg taagcttgat tgattagatt ggcaagataa
    27721 ttagccgaac gtattcttgc ttggctgtcc attccagtat tgtcatcgtg tcctgcccaa
    27781 ctactgatag tgatagatgg agttgagacg atcagccatg aatctcggta ttcattggta
    27841 gaaccagttt ttccgaccca atctgcattt cctagatttc cgtttaagct tgagatgaca
    27901 tctttgaatg gtgtagtgat tttttcattg atcacactac gcatcatgtc attcatgatc
    27961 gatgccgttg cttcggaata aatacggaca gaattacttt catgtttata aatcacatta
    28021 cctgcattat ctgtgatgga atcaatgata tagccttctt cgtagactcc accatttgct
    28081 aaggcctgga atccatttgt ctgttgaagc gtcgttacat ctactgtccc taaaggagcc
    28141 gattcaacgc cccaattatc atttgctggg tagttcatct tcgccaagta gtgctcgtaa
    28201 gcgtattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata agctgggata
    28261 ttgttggacc attccagtga ctcacggacg gtttggaagg tgttcgaccc ttcattggtc
    28321 gcattgacga ttttctcacc agcatttttt ccttcttgcc aagtcgttgc ataatctgat
    28381 acacgggatt ctgatccgac aagtcccata tcgattgcag gaccgtaaac aagaaccggt
    28441 ttgatggatg aaccagcttg acgttctgta tcaaaggcgt ggttattttg attctggctg
    28501 taattccgac cacccacgaa gccatagata cgtccggttc tgttatccat caatacattt
    28561 ccagtctcca cttggctacc agcaccatca tctaacatgt agccataatt ttctacgccg
    28621 gtttgcaatg cagcatagat atctttatca atcgtcgaat ggatcgtata tcctttgttt
    28681 tggatcgttt gctgtgcttt ttgataataa gcttcactga ccgatgaatc tgacatatca
    28741 gcattatctt tttctgccaa ttgttgtgca ataatccttg ttgcttcgtt catcacggta
    28801 taatacaaga attctctgtc agtctgttcc gcgatttgtt gtgggagaaa gtcttttgta
    28861 agatcatagg ctttcgcttc ttcgtattgt tctttcgtaa tttgtccttc acggtacata
    28921 ctgaataaaa caaaatcttt tctggcaaga ccggcagaaa gatcttcttt tagcgcgcct
    28981 gtattcgtgt atggactata cgtgataggg ctttgaggca aaccagcgat ataagcagct
    29041 tgcggcagag tcacatcctt tgcatctaca ccaaagattc ctctagcagc ttcctggact
    29101 ccagcaatat tttgcccctt attattccga ccaaatggtg agacattcaa atacgtagta
    29161 atgatctcat cttttgaaaa gtgtttttct acttgtgcag aaagaacgat ctcgtttgct
    29221 ttacgtttga atgttgtttc atcggttagg atctgttgtt tgactaattg ctgtgtcaaa
    29281 gtggatcctc cagacgagcc gatgccagtc gcttctgaaa ctagtgctcg taaaacagct
    29341 ttagggacat aaccttggtg tttatcgaag tattcatctt ctgtactgat aatcgcggtt
    29401 ttcaataaag gcgaaatctt atcagatgaa atagtcgtcc gcatcagatc tgtctggatt
    29461 tttgatatct ctgtattatc tgcataaaca atctttgacg tttccgtaat gtttcctagt
    29521 tctgtctgca gttctttttt agtcggcaat tgtgtgtctt cgacaagata ggcaaagtag
    29581 ccagcaccta ttccgatacc tagagctgcg aacaacaaaa gaagaacagt aataaaaaca
    29641 gtcaatgatt gaaacactct aaaaataatc ttaggaacaa accatttttc tttttttctt
    29701 ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac taatggtttt
    29761 atttcgctaa ttatagcaaa taataggaca aaaaaacaga caaggctgtt tttgacagga
    29821 atttcttttt cgaatgagat aaaacgtgag gaaatgatct tttttatgaa agagttctta
    29881 agcttttctt tagggaaagt ttcagttact atggagaaca gggcaaagaa aaacaaacta
    29941 ttttctttgt acatcatctt atcaatatac aggaattgtt cgaaacaatt gcctttcaag
    30001 gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga aaatgctgtt
    30061 aggcgcggca gaagaagcag ccagttacgg atcttcgacc tttatgatct atacaggcgc
    30121 tcctcaaaat actagaagaa aagctatcga agagatgaat attgatgccg gaaaagcatt
    30181 tatggcagaa aacggactaa caaatatcgt cgttcatgcg ccttatatca ttaatttggg
    30241 aaatacgatc aaaccagaaa tgtttccatt tgctgtacaa tttctgagag aagagattgt
    30301 ccgtgcggaa gctttaggtg ctactcaaat cacccttcac ccaggcgctc atgtaggagc
    30361 aggggcagaa gctgggattg ctagaattat tgaggggttg aatgaagttt tgacaaaaga
    30421 acagacggca cagatcgctt tggagacgat ggcagggaaa ggtacggaaa tcggtcgaac
    30481 atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat tatccgttac
    30541 ttttgataca tgtcatacga atgatgccgg atataatata aaagaagatt tttcaggtgt
    30601 aatggaagag tttgataagg tcatcggatt agatcggctt aaagtactgc atatcaatga
    30661 ttctaaaaat cctcaaggtt ctcataaaga tcggcatgcc aatatcggtt ttggaacaat
    30721 tggatttgac acattgaaca aaatcgtcca tgatccaatg tttgacaaca tttcgaaaat
    30781 attagaaacc ccatatgtcg gtaaagataa aaagaaagct tacgcaccat atggaaaaga
    30841 aatttccatg ttcaaagcgc aagcattcga tcccgaagtg ttccctgagt tattagcaga
    30901 agaagtcaca ctttaagttt tctacctgac agcaacaaaa aaggattggg cagtctgcct
    30961 tgtccttttt tgtttaagaa acaaaggagt gctttttgca ttcgttttcg ccatgctcct
    31021 tttgccccct ctttttaaat tcagacaaac agggtatact gatagaaaag taaaaaacga
    31081 aaagtttcac aaacaagtag caaaacgtga ttgaaaggac gaaaagatgc tgaacatttt
    31141 aaaccccttg atcgaccaac taaaaaagaa acatacatac tttattcttg gcatagccgt
    31201 attatttaca attattcggt tgtatttata tagtaaagca atctatggaa cagatatgtc
    31261 ggcgacctat gatgatcagc tgctgattca ctatgctcaa aatattttgg atggtaaatg
    31321 gctaggggag tacacgacta cgacactttc aaaagggatt tcctacagct tgtttttagt
    31381 acttgcacat aaactgcatc tttcttatgc agttttattc ggcatgttca gtattcttgc
    31441 cagtatattg gttgcactag cgtttaaacc aatcgtgaag aacaagtatc ttcttttaag
    31501 tatctatttt tattttttgt tttctccgat tagttttaca catgaatatt ccactcgaac
    31561 atatcgtaat gctctcgtga tcccgactgt tgagatcatt atcgcttgtt tactagctat
    31621 ttattaccgc aaatataatt ccagcaaaaa attgctgccg tggtttttag ggctagctgt
    31681 ttcttttcct ttcttttggt tcattcgcga agattccatc tggcttcttc cttttactgt
    31741 agtcgcgtta ggcatcagtt tcgttcagat cttgctagga caagactttt ctttcaaaga
    31801 agcaaagaag ttaaaagaac gaccatttct cctttcgaaa caagaatggc tgaaactttc
    31861 attgttttta ctccctctag tcggtttctc catcgtctat cagtggatcg aatataaaaa
    31921 cgagacaaca tatggtattg cagccatcaa tgaccgttcg agtggcgaat ttggacgttt
    31981 gatgaaacag ctgatccgaa tagatgacga aacaaactta aataaagaaa acagtgagat
    32041 atgggtctcg catgctgcat taaataaagc tttagacgca tctcctacat ttcatactgt
    32101 ttctactcgt attgacgaat tgtatacgac tcatgcgtgg acccaaggcg gaaaagtaaa
    32161 agaattacca ggagatatca ttttttgggc attgcgagat gtggtaagcg aatctggtta
    32221 ttaccaaaac aatgcaaaag aaaccaatga tttttggaaa aaagtcaatc aagaattaga
    32281 agaagcctat caaaagggaa ctatcaaaaa gaaaaaagaa ttctatctga tgggatcagg
    32341 ggatggaaag acgatgaatg atctgccaat cgtatttgat tttctgaaat caggatatgc
    32401 ttatgcatgg cgatacaaag atttccatca aggggggaat tatagttttg ggacgcaaga
    32461 acaagtcgaa caggcacaag aattattgaa tcttccacta atggataatt ggatcgatag
    32521 cagtgatccg agaacacctg atttccagtt gacaaaaaca gcaaaagtcg cgaatgtcat
    32581 gataaaaatt taccaagttc tggccattcc cgtgctgatt gcttcaatag cagggatatt
    32641 acttatgata ctaggaaccg tatttgcaaa agctgatcgt gcctattata gttcctcttt
    32701 gattgtagtc atcggattaa tactgacaca attggttttt cttttcggtg tctcgtggtt
    32761 ttgttcttat gcgccagaaa aaaaggatta ttttttaagt gtctacactg gcgcaggcgt
    32821 gccattaacg caattcatcg cagtaacaag catgctagtt tttagtaaat tgaatctgaa
    32881 aaaagcttta tcttccaaat aaaaagggaa ctgtgacaaa agtcttgtcg cagttccttt
    32941 ttccgaataa acggtgctca aaagcttgca tctgcggtaa taagctcaac caaacgtaaa
    33001 atatgaggaa ctatttttgt ttggctgttc ttattacttg atgcagggca gctttttcac
    33061 aacctctgat acacggtgtt ccagaagtaa cttcttcgga aataagccga aatttcacaa
    33121 aaatttgaaa agcaattttc ttaaattcct tcttatttct tgaagctgcc cacttctgtc
    33181 acaacctcct ttgtgtaaaa gaatcagttt ctattcgatt tcttctatcg tttcttgcaa
    33241 acgctgttca tattttttgt ttcctttata taaatcacca atcgtccatg ccaacaggag
    33301 aaggacaaga acaatgatta cataggcgat ttgatcagcc aatgctagtt gagctgaggt
    33361 ggcttgctcg ccgaagaaac tttcgatttg acctggtaat cctcgtaagt tcaagtaagt
    33421 cagactaatc acagagaacc agccaagaag ttttacaaca aatgaatttt tgaatcggct
    33481 ccccatctcc gcttggctat tggtcatcat cagtaaagga atcatcgaga atggcaatgc
    33541 aaaagccaga aaaacttggg attcattcat caagttattc aaagcggtat gttcttcgat
    33601 cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag aaagcaatcg
    33661 tgtgactagt cttcttagcc aaatcgggat acgcatatgg atgaatcctt ccatgatgac
    33721 ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa gtgctacagc
    33781 aaataaagta gacaagatac cagattttgc tacagcaatc aagataccat tgctcatagc
    33841 tgatggatca gataaagctt catacagacc aaaaaaggaa ggatcttgga cagaaccggt
    33901 tttgaataca gcgacaccca tgatcaatag cagtgcattg actaaaaaag ccattgacaa
    33961 ctggatatta gagtcccaag tagaaaagcg taccgcttgt gctacatctt cttcgtcttg
    34021 gtgatcgatc ttccgagttt gagaaactgc tgaatgaaga tataagttat gcggcatgac
    34081 tgttgcacca ataatcccta acgctccagt cagcggagtt tcaccaccta tgacaggtga
    34141 gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc aatcagggtt
    34201 agacaagcca acttgataga cgaaaacgaa taaaatgacg aaaatcaagc agacaaccaa
    34261 agcttcgatt ttacgaaacc cgacttttgt cagcaataaa aggacgaaaa catctaacac
    34321 agtgataaat acggcaatta ccaatggaat atcaaaaagt aagtagaggg cgattgctgc
    34381 accaatgact tctgcgatgt ctgttgccat aatggctaat tcagtcaaga tccataaaat
    34441 gacacccaat gtcttacttg tccgtgctcg gattgcttgg gccaaatcca tttgggtcac
    34501 gattcctaat tttgctgcca tatactgtaa aagcatagca attagactag acatcaaaat
    34561 aactgacatc aataaatatt ggaaattttg tccaccagtg atcgaagtgg accagttacc
    34621 tggatccata taaccaactg ctactaatgc acctggtcca gagtaagcaa gaagtgtttt
    34681 aaaaaaactt tttccctttg gtacatccac tgttccattt acttcagcta aagataaacc
    34741 atttgtatgt tcaataagtc gatgcttatg aaattgtttg ttggattgat tattcaagaa
    34801 aaaaccacct ttcctattta tctacgatta cattctacac ctcagaaaga aaaaattaaa
    34861 gaaaaagatt cggtatgcct aatttttttt cgtggcgtgt cttgctggtt aatgaaagaa
    34921 attttgttct tctctaaaaa aaccactatt ttttgcatga aactaaagaa tacgcctttc
    34981 ttttttcaat aaactttagt aaaataaatc attgagagca attcggaaat agaaaggaga
    35041 gatttatatg gtatcaacag gtattgtcgt acttattgct attattgctt tgctaatcgg
    35101 cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc
    35161 tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atggggcaaa aaccatctga
    35221 gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac
    35281 ccaaagactt tttgcgggct gattttttca gtccgttttt tatttcgtaa tttgttagac
    35341 tattcagact attcagcaaa aaagacaagg acagacaatc ctatgctctt gataaagaca
    35401 aaggaggagt aaaatgtcga tttttagaaa attaggttgg ttttttcgtc aggaaaagaa
    35461 acattattta attgggattt tctccctatt cgcagtggcc ttggtacagt tagttcctcc
    35521 aaaagtgatt gggatcatca ttgatgagat cgccgataag aatttatcaa taaagatcat
    35581 ttcttggtgg atcttactat tgatccttgc agctttctta caatatcttt ttcgttatat
    35641 ctggcggacg aatatttggg gaagtgcagc tcggttggag aaagaacttc ggcagcagct
    35701 attcaaacat tttacagaaa tggacaatct cttttaccaa aaatatcgga ctggagattt
    35761 gatggctcat gcaacgaatg atttgaatgc aatccagaat gtagcaggag ctggaatctt
    35821 gacctttgca gattcagtga ttacgggcgg aacaacgatc attgcgatga ttttgtttat
    35881 tgattggcgc ttaacactga ttgcgctgat tcctctgcct ttgctagccg ttacttcacg
    35941 agtgctgggg tcaaaactcc acgatgcttt tcgtgattcg caagaagctt tttctagtat
    36001 caatgataag acacaagaaa gtatcactgg gatcaaagtc attaaaacat ttggacaaga
    36061 aaaagaagat ctcgcggatt ttgaagaaaa gattgaagat gcaattatta aaaacaaacg
    36121 agtcaatttt ttagactcct tatttgatcc attcattacc ttgattatcg gtctgtctta
    36181 tgttgcaaca attattttag gtggaaagta tgtcttggaa ggcacgatct cgattgggca
    36241 gatggtctca tttattagtt atatcggtat gcttgtttgg ccgatgtttg cgattggccg
    36301 attgttcaac gtattggaaa gaggaaatgc ttcttatgat cgcgttaatg aacttttgca
    36361 tgaaaagacg catatcgtgg aacaactggg tgcagtgcag accccggcaa aaggcgagct
    36421 gaatgtagct atttcctctt ttaactatcc gaaagaagaa tcaccagcat tagaacaaat
    36481 caaatttgcg atccaagaag gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa
    36541 aacgacaatc atcaaattac tgatgcgtga atacgatgat tatcaaggga aaatccaatt
    36601 tggcggacgt tcgatcaaag aatattcatt gaatgcttta ttaggatcaa tcggttatgt
    36661 gcctcaggat cacttcctgt tttcgatgac cattcgagat aatatccgtt tttctaatcc
    36721 aaaactttca gaagaagaag tagagcatgc ggcttcttta gcatttatca acaatgaaat
    36781 caaggaattg ccaaaaggtt atgatacctt agttggagaa cgtggagtct ctttatctgg
    36841 cggacagaaa caaaggattt cgattgctcg agcattgatc aaaaatcctg aattgctgat
    36901 cttagatgat gcactttctg cagtagatgc caaaacagaa gaagctatct tgcataattt
    36961 aaaagaaata agaaaagaga aaacaaccat cattgcagct caccgtttaa gcagcgtgat
    37021 gcatgcaaaa gaaatcattg ttgtcgatca agggaaaatc attgaacgag gaacccacca
    37081 cgagctgctt gcattaggtg gctggtatga acgaatgtgg gaaaaacagc aattagaagc
    37141 gaagatagaa ggaggcgagg cctgatggat gaaaattatc aatcggaatg gacaaaatcg
    37201 attccactta aagaacagaa gaagatcatc aaacgactga tgaagttcgc caaacctttt
    37261 agaagaacgt tcatcgttgc tatcttattt gcgtttgccc tgtctgtgat caatgtccta
    37321 ttgccaagga tcatccaaac ttttatggat gatcatctgg ctaaacagtc agctacgaca
    37381 caagttattt tgttttttgc tggtgtgtac ttgtttggag tcatagtcaa aagtatcatt
    37441 tggtttttcc aatggtattt gtattccatg gcttcattga agacctatca gtatgttcgg
    37501 gtaagattat ttgaaaagct gcatacacta ggtatgcgtt attttgacca gacaccggca
    37561 ggatcgaccg tttctcgagt gacgaatgac acagaaacgc tatttgaatt ttggtatgtc
    37621 tttttgatgg tattgactgg gatctttgca gtcgtttctt ctttcgttgc catgtttcaa
    37681 atcaatggag aaattgcctt gtggactttg gtgttcttgc cgattttagg aatcgtcatc
    37741 tggtattacc agaaattcag ttctcgtatt taccgaaata tgcgggaaaa attaagtcag
    37801 ctgaacacga aattgaatga atcgatttcc ggcatgcgta ttatccagca gtttcgccaa
    37861 gaagctcgtt tagctaaaga atttgaacaa gtgaacaacg agtatttaga cacgcgctat
    37921 gcgatgatca aaacgaattc gattctgtta agtccgatca tcaattttct ctatgcattg
    37981 gcgattgcgt tggcgttgac tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt
    38041 ggactgatct atgcatttac gacctatgtc cagggatttt ttaatccaat gtctcaaatg
    38101 atggactttt tgagcacctt tacagatggg atggttgccg gtagtcgtat tttgaaaatc
    38161 atggatacag aagaatatgc accgcaacaa aatcctggag cagatcaagc gattacttca
    38221 ggtaagatcg agtttcgcaa tgtaagcttt tcttatgatg gagagcacaa tgtattgaac
    38281 aatatctctt ttgtcgctaa tccgggtgag accgttgcat tagtggggca caccggaagc
    38341 ggaaaaagct cgatcatcaa cgtattgatg cggttttacg aattttatga aggccaaatc
    38401 ttgattgatg atcatgacat tcgagaatat ccaatggaag agttacgtaa gaaaatggga
    38461 ttggtcttgc aagatgcctt cttgttttat ggagatattg caggaaatat ccggatgatg
    38521 aatccagcta tcacagacga acagattcgc catgcagctg agtttgttca agcaaacaag
    38581 ttcatcgatg aattaccaaa aggatatcac tcaaaagtga tcgaacacgg tgccagctat
    38641 tctagtggac aacgacagct gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata
    38701 ctagtattgg atgaagcaac agcaaatatc gatactgaaa ccgaaggatt gatccaagaa
    38761 ggattggcaa aaatgcgaca aggacggaca acgattgcga ttgcccatcg gctgtccacg
    38821 attcgggatg cagaattgat tttagtttta gacaaaggtc atatagtaga gcgtgggaat
    38881 catgaaacac ttctagcgca aaatggtttg tatgcagata tgtacaaact gcaaagcagt
    38941 ggtgtgtaaa aagaggttgt gacagtagca tagtagcatt ttgtcctaag cgctagagtg
    39001 aaaataaaac agagcttccc agattcttca tctgtgagcg ttagtggcgt agtcacccga
    39061 tttaaaaaag acgacgacag ctagtcaaaa gtgcctaata gccactaaga aagcgttgac
    39121 aaaagcgttt gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga
    39181 tgcaagctaa acctgatgag tctcccatgg ttccatggac ggctttttgg gtttttcttt
    39241 ttgttttgaa tacagcccta tatatcatgg aaaggagcaa aacatgctga ttgagaaagt
    39301 actgaataac aacgtcgtaa taaccaggga tgaaagaaat caggaaatga tcgtgatggg
    39361 aaaaggtttg gcattcaaaa agaaagtggg tgacccgatt tttgactcat gcatcgacaa
    39421 aaaatttcag ttgacaaatg acgggctttc acaacagttc caagaattgg cacaagcgat
    39481 tcctcttgag tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctaca
    39541 agtccagttg aatgattctg tgtatattgc attgacggat catttgtata cggctatcga
    39601 acgtcacaaa atgggaatat ttgtaccaaa tgtattgctg tatgatatca aacaactatt
    39661 tcctaaagag tatgccatcg ggaaaaaaac agtgaagaaa atctcggata aataccacat
    39721 caagttatca gaagatgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat
    39781 tcaaagccaa caaaacaata cctacctgtt tactataaca atacaggata ttctaaatat
    39841 cgtccgctat tactataaga ttacatttga tgaagattca gtccattttt ttcgattcgt
    39901 cacacactta cgtttttttc tgacacgcgt cctagatgga gaaaaatcag aagtgggaga
    39961 agtttctgag gaattgtttc aggttattca aaaaaaatat aaggatacgt ttggttgcgt
    40021 tgagaaaata gcagactttt tagccgagac gttcaactac cagatgtcca cggacgaaaa
    40081 aatgtattta acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaataaa
    40141 aaaaggagga taaattaaca agaacgtatg aaataagggt agtgacattc agttggctaa
    40201 accttccact aatatattgg agggaataga aatgggaaaa taccaagaat tagcgagaaa
    40261 aatcgtagaa aatgtcggtg ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag
    40321 gcttcgtttc aaattaaaaa acgaagaaaa agcaaatgat gagatcttga aaaatatgga
    40381 cggtattgta acggtaatgc gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt
    40441 gccagtcgtg tttgaagagg ttataaaagc aggaaacctt acttttgatg aggctgtttc
    40501 aacagaaaaa atgagaccgt ttgatatgct tattgatgga atatcgggct gtttccaacc
    40561 attccttgct atcttagctg ctggcgggat gattcgtgga ctgacagcat tccttgtctt
    40621 cttaggcgcg tttgaccgcg gttctggcac atttgttatg tttgataata tcggtgattc
    40681 tgtattccaa tttatgcctg ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa
    40741 tgaattcgta gggatgttgc ttggcgcagc attaatgaat cctagcttgt cgctggaagc
    40801 tttgagtggt gcagcagaag caccactaac gaccatattt tctggcacta tttttgaagc
    40861 acctatttat caaacagtat tcggcattcc ctggattgcg cgtaactacg cttcttctgt
    40921 cattcccatt atctttattg tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt
    40981 agtacctgag atgatcgcga atttcttcgt tccattcttc accgttctta ttacaatgcc
    41041 tttaggtttt ttactcgttg gaccagtatt cacattcgca actgatattt taatggctgg
    41101 atttgaaaca cttttagcac tttcgccagt tatttatgga gcaatcgtag gcttcttctg
    41161 gcaaattctg gtgatgtttg gtctccattg ggcaatcgta ccaatgggat tgatgcaatt
    41221 ttctgtgaat ggttggcaaa atattatgac acctgttgca gtagtgtctt ttggacaaac
    41281 tgcagcactt accgcactct actttaagct tagaaatcca aaagacaaag caattgcaat
    41341 accagccatt gtttctggta ttgtggggat tacagagcca gctatttatg ggtttacatt
    41401 accacgtaaa aaaatattta tttttacttg tgtcggtggt gcgttagggg gcgcgtatag
    41461 tgggttaatg aatttaactt cttggaatca aggtggacta ggcatcttta caattcccaa
    41521 ctatattaga ccagatggtg atttaacaga tgtgattaat gtattgattg ggatcgccat
    41581 tgcgatggtg gtatcctttg cgctgacttt tttcttttgg aaggaggaag caggtgaaac
    41641 agatgacaga cagaaaagat caggtaaaga aattgtaaaa acaccgatac aaggacaaat
    41701 cgctccactg aaagcagcaa aggatgcagc ttttgcacaa ggaacactag gtcgaggaat
    41761 cttgatctac cctgaaaaag gagaagtacg tgcccctttt gacggaacag tcatgaccct
    41821 gttccccacg aaacatgcaa tcggaatggt ttctgaaaca ggtctggaat tgctgattca
    41881 tgtcggattg gatactgttc agttggaagg gaaatatttt gaaagcttgg tgcagcaagg
    41941 agaaagagtg aaacaaggcg atctgttgct ccgcttcgac aaaaagaaaa tcgaaaagga
    42001 aggatacagt ttagaaacac ctgtaatcgt caccaattac atggattatt tggatatttt
    42061 ggaaaatcaa ggggagacgg tcgggccaga cgatgcactt atcaccgcac tgacctgaac
    42121 ggttaggtga aagaatggaa tagacttaac tattcttttt agtccacttc tttattttca
    42181 aaattgaaag gctctttcat gatggctgag ctgatagggc gtttatcaaa tttttgtcct
    42241 acagaaatta gatggactaa attctgttga taattttctg caaaaacaca actatataac
    42301 acgtctagaa gataacaaat cgatgtattg atcgtaaaat taccaatttt tgaatacagt
    42361 cgttcgcgtg tcgtaagtcg caaagcggct gtactcattg aggatagcgt tgaatcaccg
    42421 atactggtca acgctaaaat tggaacatga ttttgtttca atatttctgc ggtacgaagg
    42481 atcacattgt tttcacccgt ataagaaatc agaatcccgc acatatcact cgtgcaattg
    42541 tacgcttcat agaacgattc accaaaagtg gtgacaatcg tgacatccat tttgatgcgc
    42601 ttcattttca gcatgaaatc ttgagaaatc aaagtattcg agttactggc gaaaatcacg
    42661 attttttttg cttgaagcat tagttgtttg gctttttgca gttggtcatg atgaatgaga
    42721 gaaagtgtat cttgaatcgt catctgttct aaagaagcca ttttatttgc gatcgtcata
    42781 ataccgtctg tggtggaaaa aggaagattc gcatctacgt cttgaaaatt cccttcaaga
    42841 taggctgttt cagctaaaaa agcattacga aaactgaccc agccatcaaa acccattttt
    42901 tttgctacac ggatgagtgt ggacgggtgg acataacatc gctcagcaat ctcttggatg
    42961 gtcatttcgc caagtgctgg agaatcgctt aagagaaact gaatcacttc attttctgaa
    43021 ggagaaaagg agatttcttt gattttttca cgtaataaca tgtagatacc ttcttctagt
    43081 ttttttacgt aaattataac ttttttgaaa atgatttgta aacggattta tgttatatag
    43141 cttaataaag tcattttttg acaagatatt tacaaaaatc cagtagaaaa cactaacttt
    43201 tgtaaatgtt actggaaaga aacgaatttc tattgtttgt tttgtttaca aaaaagataa
    43261 taaaagtgta aaaagcaaag gagtgagaac atggcaaaag gaatcaaaat cgctacgatt
    43321 ggtggaggca gcagttacac ccccgagttg atggaaggat ttatcaaaag atatgaggaa
    43381 ttaccgatac gagaaatctg gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc
    43441 gtcagtcaga tgtctcagcg tatgtgggat gcttctccgt atgaagtcaa gatctatgca
    43501 actttggatc gggaagaagc attgaaagat gcggattttg ttacgactca gtttcgtgtc
    43561 ggtcaattag atgcacgaat caaagacgag cggatcccta gttattatgg gatgattggg
    43621 caagaaacaa atggtgctgg cgggatattc aaagcttttc gtaccgttcc agtggttttg
    43681 gcaatcgttg aagacatgaa gcgtttatgt ccaaatgctt ggctgatcaa tttcgcgaac
    43741 ccaagcggga tggtgacaga agcagttatc agatatggca aatgggataa agtgatcgga
    43801 ttatgcaatg tgccagtaat ggcgacgatg acagaaccag caatgcttgg caagaaaaaa
    43861 gaagagctga tctacaagtt tgccggtcta aatcacttcc attggcacaa agtagcagat
    43921 aataaaggaa atgaccgcac agcagatttg attgatcgtt tatacgatga agacaatgga
    43981 ttgccgaaaa atatctttga catcccgttt ttcaaagaac aattaaaaca aatgaacatg
    44041 atcccttgtg gctaccaccg ctattactac cgtgaagaag aaatgttgga acatgcttta
    44101 gaagaatatc aaacgattgg tacaagagcg caacaagtga aagaaacaga agcagagctg
    44161 tttgaattat acaaagatcc agaattggat cataaacctg aacaattgca aaaacgaggt
    44221 ggtgcttatt attcagatgc tgcctgtgaa acgattgctt cgatctatgg aaataaagaa
    44281 acacagatcg tggtgtctac aaagaatgat ggagctgttc ctgatctgcc ggcagattgt
    44341 gtagtagaag tcactgccta tgttggcgca caaggcgcac gaaatgtagc gtttggttcc
    44401 cttcctgctg ctgaaagagg ctggttgcaa gtaatgaaga atatggaatt gctgacgatc
    44461 gaagcagccg tgactggtga ttatggaaca gctttacaag cgtttacgat caatccgctt
    44521 atacgatcag gaaagacagc aaaacggatc atggatgagt tgtttattgc acatcagcag
    44581 tatttaccgc aattcaagga aacaatcagc cggttaaaag cagaaggaat cgaagtccaa
    44641 gatgaactgg caagagaatt gtcaagcaat acacatgtaa tggaggaagt atgatgggat
    44701 tcagaaaaga ttttttatgg ggcggtgcta cagcagccaa tcaatgcgag ggtggttatg
    44761 atgaaggcgg acgtgggtta gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc
    44821 ctgtcattac tggaaaaaag aaaatgtttg aatttgataa agatcatttt tatccagcaa
    44881 aaattgcgat cgatatgtat catcattata aggaagatat tgctttgttt gcagaaatgg
    44941 ggttcaagac ttatcggatg tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa
    45001 atgagccaaa tgaatctggt ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt
    45061 acgggatcga gccgcttgtg acgattacac attttgattg tccgatgcat ttgatcaaag
    45121 aatatggtgc gtggcgttct agagaactcg ttggttttta tgaaaatctg tgccgagtca
    45181 tcttcaatcg ctataaagga cttgtcaaat attggttgac attcaatgag atcaatatga
    45241 tccttcatgc accattcatg ggcgcagggc tgtattttga agaaggagaa aatgaagaac
    45301 aaatcaaata tcaagcggca catcatgagt tggttgcaag tgccatcgca acgaagattg
    45361 ctcatgaagt agatccggag aacaaagtag gctgtatgct ggcagcagga gcatattatc
    45421 catacacttg caatcctgaa gatgtttggg cttctagaaa atcagatcga gaaaattatt
    45481 tcttcattga tgtccagtca cgaggagaat atcccgctta tgcattgaaa gaaatggaac
    45541 gaaatggcat tcagatcaaa atggaacctg gggatgccgc attgctgaaa gaacatactg
    45601 ttgattttat ctccttttct tattattctt ctcgagtttc gactactgac aacgaactct
    45661 tggaacagac tgcaggaaat atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg
    45721 aatggggttg gcaaattgat ccattaggct tgagaataac gatgaatgat atttatgatc
    45781 gttatcaaaa accactattt attgtagaaa acggcttagg tgcagtcgat acgcccgatg
    45841 agaatggtta tgtggaagat gactatcgaa tcgactatct ggcagcacat atccaggcaa
    45901 tgaaagatac agtagaatta gatggagtcg atctattagg ctatacgact tggggatgta
    45961 ttgatttagt atcagctggt acaggtgaga tgaagaaaag gtacggattc atttatgtcg
    46021 atcgtgacaa cgaaggaaat ggcacattag cccgttcgaa gaagaaatca ttcaactggt
    46081 ataaaaaagt aattgattca aatggagaaa atctttcgaa tgattgatca atgatttgta
    46141 aaagtctatt gtttttcagc ggatttttcg agtataataa agctattcga gaaacgcaag
    46201 cagaacaata gacttttttt cgcattggct gtaaagtatg cttacttgat taagtaaagg
    46261 agaatctaca tatgtatgat gtaatcgtta ttggcgcagg tcctgctggg atgactgctg
    46321 ctttatatgc ttcacgttca aacttatccg tattgatgat cgaacaaggc gcccctggcg
    46381 gccaaatgaa caatacggca gaaatcgaaa actatcctgg tttcgattca atcatgggtc
    46441 ctgatctagc ttataagatg tatgaaggtg tttcaagatt tgggacagaa aatgcttatg
    46501 gaatcgtgca agatatcaag gatcacggca gccacaaaga agttatttgt gaagataaaa
    46561 cttacgaagg aaaaacagtc attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac
    46621 ctggcgagga agaatttgct ggacgtggcg tttcttactg tgcagtttgt gatggtgcgt
    46681 ttttccgtaa caaacgtcta gtagtgatcg gaggaggaga ctcagcagta gaagaagcca
    46741 tctatttgac acaatttgct tctgaagtag tcatcgtcca ccgtagagat gaattacgtg
    46801 cacaaaagat catccaagac cgtgcatttg cgaatgaaaa aatttccttt atgtgggata
    46861 cagtcgttga cgaaatcgtt gggaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg
    46921 tcaaaaccgg caatgagttt gatttggcag cagacggagt attcatctat gttggactgg
    46981 atccattaac tgcaccattt agagaagcag ggttgacgaa cgaagctggc tggattccaa
    47041 cagacaacga aatgaagacg aatataccag gcgtttttgc catcggagat gttcgtgaaa
    47101 aagatttgcg acaaatcact actgctgttg gtgatggtgg gatcgctgga caacaagtgt
    47161 ataaatactt ggaatcttta gaagaaacaa ctgcataagt ttaaaaagag tggaagaacg
    47221 gtttcaattg ttcttttact cttttttttc gcgtgaaatc tactgaatcc gatttatgcg
    47281 aatacttgca accgtgcttg ataaaagaat cctttcttga tttaagcgtt ttttagacct
    47341 cgtactttca tatctaataa gtgaatgcta tacttattaa gaaccatatt tcagaaagag
    47401 gtgttatttt catgtcttgg gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag
    47461 aagaaaactt aaaaaaacaa ttaattgatc tgggtgaaga tcctgaaaaa cttgaagatg
    47521 ctttttatgc accgttggaa ttcggtactg caggcatgcg gggaatctta ggacctggga
    47581 tcaatcgaat gaacatctat acggtaagac aagcaacaga aggacttgca cgtttcatgg
    47641 atacacaagg tgaagaaaca aaacgccgag gtgtcgctat cgcttatgac tcacgccatc
    47701 aatcaccaga atttgccatg gaagcagcca aaacattagc acatcacaat atcccttctt
    47761 atgtattcga aagtttacgt ccaacaccag agctttcttt tgcagtccgt catttgaaga
    47821 cgtttgcggg aatcatggta actgcatccc acaatccagc agcttacaac ggatataaag
    47881 tctacggaga agatggggga caaatgcctc ctgcagacgc agatgcattg acgaaatatg
    47941 tccgtgaagt cagcaatccg ttaaaagttg aagtattatc tgacgacgaa gctgaacata
    48001 gcggattgat cacgatcatc ggagaagaag tggatgccgc ttatctagaa gaaatcaaag
    48061 cagtaacgat tgatcatgaa ttggtggaaa caatgggtaa agatttgaaa cttgtgtaca
    48121 caccacttca cggaacagga aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg
    48181 aacaattcgt actagttcca gaacaagctg tcgcagaccc ggatttcact acagtcaaat
    48241 caccgaatcc agaagaacac tctgctttcg agtatgcgat gcgacttggc gagaaagaag
    48301 gcgctgactt actgattgca acagatcctg atgcagaccg tttaggcgca gccgttcgtt
    48361 tgccaaatgg ctcctatgaa gtattgacag ggaaccaaat cggagccctg tttgctcaat
    48421 atattttgga agcacaccaa tcgcgaggca ctttacctga aaatgcagcg gtattgaaat
    48481 caatcgtttc tagcgaattg cctacagcaa ttgcggaaca ctatggggct accatgttca
    48541 atgtattgac aggattcaaa ttcatcgctg aaaaaattca acaatttgaa gaagatcatt
    48601 cccatacttt tatgttcgga ttcgaagaaa gttacggtta tttagttaaa cctttcgttc
    48661 gcgacaaaga cgcgattcaa gcattgctct tattagcaga agtagcagct tactataaaa
    48721 aacaaggcaa aacgttgtat gacggcttgc aaacgatttt tgaaacatat ggctactacg
    48781 cagaaaaaac gatttctgtg acaatgagcg gacaagaagg ttctgcaaaa atctctgctt
    48841 tgatggaaaa attccgtgaa gaagcaccga cagaatttgc cggagcaaaa gtcatccaaa
    48901 cagaagactt caaagagttg acgagaacag accgtgaagg aaacgtagaa aaactaacga
    48961 caccgccatc tgacgtattg aagtatacgc tggaagaaaa tggctggatc gctatccgtc
    49021 cttcaggaac tgagccgaaa atcaaatttt atatcggtgt aaaaggaaac tcttcagaag
    49081 acgcagctga aaaagtagcc gcttatgaag cgattatcaa aaaaatgaca gaagaataaa
    49141 aaaacttgag actgatggaa aaagtggtaa actttagaag tcagtctttt ttcaagttgg
    49201 aggtgaatgg attgccagac acgcaaaaaa caaaagtaga gaaagtcagc caactattca
    49261 aaatgttgag tgaccccact cgtctcaaaa ttctgctgta tctgaaagac ggggaacaaa
    49321 atgtgacagc aatcacccaa gcagtagaaa tggaacaatc tgctgtatcg catcaactgc
    49381 gtttattaag agaaaaccat gtcgtcaagt cccatcgtga gggaaaagcg attttgtata
    49441 gtcttgatga ttctcacgta ctggatattt tgaaccagac gctcaaacat gtggaacatc
    49501 aataacgata ggtgcaaata gagacaaact ggactgtatc aacgaatctt cttcgctgac
    49561 acagtccagt ttttttaaga aaaagacaag aaaaactgtt agtctgtttc ttcggtggtt
    49621 tcagttccat aataactatg atcttctagg tctaaagcat tcaaaataag tgaagctgtt
    49681 tcttcgatag ataaagaagc gacattgatt actacgcagc ccaatttttc ataaagatca
    49741 ttcgcaaaat cgagttcagc tcgtattttt tcgatatcgg aataagcagt atcaggattt
    49801 aatccgtaag agcgcatccg ttctttacgg atattgttta aaatatctgg gttattcgtc
    49861 agtcccacga tttttttagg atcgatctcc caaagctgtt tgggaatatg tgcttgtggg
    49921 atcaaaggta gattggcaac ctttaaattt ttgttagcta aaaacaagct caaaggcgtt
    49981 ttggaagttc tggatacgcc tagcaaaacg acatctgctt ctagaaaccc acgaggatct
    50041 ttcccgtcat cgtattttac agcaaattcc attgctttga tccgtttgaa atagttctca
    50101 ttcaataaat gaagggcgcc aggttgccga atgggcttga taccagttaa gcgctcgatt
    50161 tctgatatcg gcggagtcat cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa
    50221 cgcgtcatct cgatcaattc ttgattgatc aatgtgtgca ggacaagggc tttttctttt
    50281 agtgcatctt gcagtgcctg catcaatgtt ttctcatctg tcacaaatgt tctgcggaat
    50341 aaattgaact caacggtagg atattgggcc atagtggctt gcgctaattt tgtcgcagtt
    50401 tctccagcag agtcagagat gacaaaaaaa gtaacgattt ctgtagaatc ggaagacatg
    50461 gataaaacct cacttgcatt ttttttatgt ttattatacc atactctaga atgtgtactt
    50521 cgtaatcatt ttgatttgga ggaataagtc atgaaaaaac agacattgat tgaacaatca
    50581 ctagaaacat tgaaaaataa cggttttaaa tatactaaaa aaagagaagc attgttaact
    50641 tatttagtga agcgcaatcg ctacgtatcc gctaaagaag tttttgacta tatgaatcag
    50701 gaattcaaag gagtaagtta cgatacgatt tatcgaaatc tccatgattt tgcagaaatc
    50761 gatttactgg aagaaaccga tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa
    50821 ggggaaatcg gccatcacca tcactttatc tgtacggtgt gcggaacaac gaaagaaatc
    50881 catatgtgtc caatgaatta tttcgaagaa cagctcggtg gatgtacgat cgaagggcat
    50941 agattcgaga ttttcggaag atgtgaaaat tgtcaataag gatttaaata aaataacatt
    51001 tgataatttt aaaaaaaagc aagatttcct tgacggtcaa aacagcatta gatataatat
    51061 ttacaggaca gtcttgtttt ttgagatata tttcaataac aattacggtt actttttaag
    51121 ctcggaggga gggaattaac atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg
    51181 atgctcttcg tcgcttcaaa cgttccgtgt caaaagcggg tactttacaa gaatctcgta
    51241 aacgtgaatt ctacgaaaaa ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa
    51301 aacgcaaaaa attctagttc tgtgatgaat tggagttggt agtgtgtcac tactaagtag
    51361 attaaacgat gacatgaaaa cagcgatgaa agcgaaagat aaggaatctt tacaagtcat
    51421 ccgaatgatc aagtcatcta tacaaaacga gcagattaaa gaaggtcacg acttgacaga
    51481 agaagaagaa ttaacggtgt tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga
    51541 gtttgaagaa gcaggacgtg acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt
    51601 cgaaaaatac atgccggaac aactttccga cgaagaaatt cgacaacttg ttcaagaagc
    51661 aatcacacaa actggtgctt catcaatgaa agaatttggc aaagtcatgg gtgcgatcat
    51721 gcctaaagtc aaaggaaaag cagatggaaa ccaagtaaat gccattgtga aagaactatt
    51781 acaatcttaa ttttatcatt t
//
LOCUS       DASHFF010000014        50350 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.014, whole genome shotgun sequence.
ACCESSION   DASHFF010000014 DASHFF010000000
VERSION     DASHFF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.014"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(58..186)
                     /locus_tag="U2I75_000936"
     CDS             complement(58..186)
                     /locus_tag="U2I75_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1696087.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="U2I75_000937"
     CDS             complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="U2I75_000937"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1696088.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1673..2728)
                     /locus_tag="U2I75_000938"
     CDS             complement(1673..2728)
                     /locus_tag="U2I75_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1696089.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="U2I75_000939"
     CDS             complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="U2I75_000939"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN1696090.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="U2I75_000940"
     CDS             complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="U2I75_000940"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN1696091.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="U2I75_000941"
     CDS             complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="U2I75_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN1696092.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="U2I75_000942"
     CDS             complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="U2I75_000942"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN1696093.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="U2I75_000943"
     CDS             complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="U2I75_000943"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN1696094.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10490..12049
                     /locus_tag="U2I75_000944"
     CDS             10490..12049
                     /locus_tag="U2I75_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN1696095.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12160..14091)
                     /locus_tag="U2I75_000945"
     CDS             complement(12160..14091)
                     /locus_tag="U2I75_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1696096.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="U2I75_000946"
     CDS             complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="U2I75_000946"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN1696097.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15020..15772)
                     /locus_tag="U2I75_000947"
     CDS             complement(15020..15772)
                     /locus_tag="U2I75_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1696098.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15931..16140)
                     /locus_tag="U2I75_000948"
     CDS             complement(15931..16140)
                     /locus_tag="U2I75_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696099.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16151..17479)
                     /locus_tag="U2I75_000949"
     CDS             complement(16151..17479)
                     /locus_tag="U2I75_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696100.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17615..17770
                     /locus_tag="U2I75_000950"
     CDS             17615..17770
                     /locus_tag="U2I75_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696101.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17844..18431)
                     /locus_tag="U2I75_000951"
     CDS             complement(17844..18431)
                     /locus_tag="U2I75_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN1696102.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="U2I75_000952"
     CDS             complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="U2I75_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN1696103.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20011..21300)
                     /locus_tag="U2I75_000953"
     CDS             complement(20011..21300)
                     /locus_tag="U2I75_000953"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN1696104.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21454..22386)
                     /locus_tag="U2I75_000954"
     CDS             complement(21454..22386)
                     /locus_tag="U2I75_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696105.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22543..23202)
                     /locus_tag="U2I75_000955"
     CDS             complement(22543..23202)
                     /locus_tag="U2I75_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1696106.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23205..24230)
                     /gene="sppA"
                     /locus_tag="U2I75_000956"
     CDS             complement(23205..24230)
                     /gene="sppA"
                     /locus_tag="U2I75_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN1696107.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24243..24533)
                     /locus_tag="U2I75_000957"
     CDS             complement(24243..24533)
                     /locus_tag="U2I75_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696108.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="U2I75_000958"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="U2I75_000958"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN1696109.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="U2I75_000959"
     CDS             complement(26820..28175)
                     /locus_tag="U2I75_000959"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN1696110.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="U2I75_000960"
     CDS             complement(28199..29368)
                     /locus_tag="U2I75_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696111.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="U2I75_000961"
     CDS             complement(29365..30252)
                     /locus_tag="U2I75_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN1696112.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34121)
                     /gene="nifJ"
                     /locus_tag="U2I75_000962"
     CDS             complement(30441..34121)
                     /gene="nifJ"
                     /locus_tag="U2I75_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN1696113.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34319..34840
                     /locus_tag="U2I75_000963"
     CDS             34319..34840
                     /locus_tag="U2I75_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1696114.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35539..36104)
                     /locus_tag="U2I75_000964"
                     /pseudo
     CDS             complement(35539..36104)
                     /locus_tag="U2I75_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36283..36939)
                     /locus_tag="U2I75_000965"
     CDS             complement(36283..36939)
                     /locus_tag="U2I75_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696115.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37507..38442)
                     /gene="whiA"
                     /locus_tag="U2I75_000966"
     CDS             complement(37507..38442)
                     /gene="whiA"
                     /locus_tag="U2I75_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN1696116.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38476..39474)
                     /locus_tag="U2I75_000967"
     CDS             complement(38476..39474)
                     /locus_tag="U2I75_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN1696117.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39471..40355)
                     /gene="rapZ"
                     /locus_tag="U2I75_000968"
     CDS             complement(39471..40355)
                     /gene="rapZ"
                     /locus_tag="U2I75_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN1696118.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40490..41221)
                     /locus_tag="U2I75_000969"
     CDS             complement(40490..41221)
                     /locus_tag="U2I75_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN1696119.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41225..42490)
                     /locus_tag="U2I75_000970"
     CDS             complement(41225..42490)
                     /locus_tag="U2I75_000970"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN1696120.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42753..45569)
                     /gene="uvrA"
                     /locus_tag="U2I75_000971"
     CDS             complement(42753..45569)
                     /gene="uvrA"
                     /locus_tag="U2I75_000971"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN1696121.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45579..47573)
                     /gene="uvrB"
                     /locus_tag="U2I75_000972"
     CDS             complement(45579..47573)
                     /gene="uvrB"
                     /locus_tag="U2I75_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN1696122.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47832..49334
                     /locus_tag="U2I75_000973"
     CDS             47832..49334
                     /locus_tag="U2I75_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN1696123.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49336..50073
                     /locus_tag="U2I75_000974"
     CDS             49336..50073
                     /locus_tag="U2I75_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1696124.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT        13904 a        10928 c         8861 g        16657 t
ORIGIN      
        1 aaaaagacta agaaacaata acaaaagtta acacttctta gtctttcttt agcacgtcta
       61 tttattaaag attttagggc cttttctcat gtttttacga tcattcttag gtgtttccat
      121 ttgtttggat cggcctccac cttgtttctt tttgatcaat tctttcattt tttctttttg
      181 gtccattttt attctccttt aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg
      241 aaaagatcta caggctgaac tttcttcacg gcataacctg cttctgcaaa taacttagca
      301 tctcttgcaa aagttgccgg attacatgag atatagacga ttcgttctgg tgacatttcg
      361 attgctgatt caataaaatg atgatctaat ccttttcgtg gagggtcaac tacgatcaca
      421 gatggtttga tccctttttc ttgccatttt ttcataactt tttctgcttt tccgacttca
      481 taatgaacat tttcgatttt attatttaaa gcattgaact gtgcatcttc aattgcttct
      541 ggaataactt ccatcccata caccttatcc actctgtctg ctaaagacaa gccaatcgta
      601 cctattcctg aataagcgtc gatcacaatg tcttttttat ctaactcggc aaactcgatg
      661 gctgtttggt acagatactc tgcttgttga gtatttactt gataaaatga ctttgctgag
      721 attcgatagg tttttcctaa tagctgatct tcgatatagt ctctgccata taaaactttt
      781 tctttgtctc ctaaaatcac atttgttttt tcttcattga tattttggat gaccgaaacc
      841 acttctggca attcttcatg aatagcttca gcaatttcct tggatttaaa gaatttttct
      901 ttacgagtaa ctaaaacgac catcatttca tgggaataat gaccacgacg gataatgatg
      961 tgacgaagct gtccttcatt tttttcttca ttatatcctc gtacttggaa ccgttgcaag
     1021 atatctctga ctactttgat tgcttgatcg atgacaggat cttggatata aaaatcttcg
     1081 atagggacta gatcatggct attctttttg taaaaaccgg tggctaactg tccatcgata
     1141 cggcgaacag ggatctgtgc tttattacgg tagccgaatg gttctgccat tccgaccgtt
     1201 ggaagtacag gaacttctgg catcttagca atcttcgcca ttacatgctc tacttgtttt
     1261 tgtttgaaga caagctgttg ttcatacttc aaatgggcta aaggtgcgat acctatgcga
     1321 agtaatgcat tgttttctac aggctgacga tcaggtgatt gtgtaataaa tgtttcgact
     1381 tttccatatc caaacttatt gcctgctttt actactagta tttctacttc ttctccaggg
     1441 agtgcatttt cgataaataa tggataacca tcgatttttg ctacccccat tccttcataa
     1501 ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct ttactatttg ctgttctgtc
     1561 atattttgct cctttaagcc tatttttcaa tcctactgaa ctctactata aatcagctaa
     1621 ttacacaact aaaaccggca ctgaccttac tacgttcagc accggctttt gattaattct
     1681 tttgattagg tgtttcgtct gctatttttc cgtcaccgtc gatatcttca tccgttaaac
     1741 gttcgacttc ttttacgaat tctttgctga caacctctaa ttcttcttca tctgtacctg
     1801 tgattgcttc atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat
     1861 tttggaaatg catcggtgct tccccgccgt attcaccatc taagttgatc attactggtc
     1921 gattgatatc atcttctgtt tcaacgaaca aactgcttgt tttggtatag attattcttg
     1981 gatcatcgac atgtcgaccg ccattcagca tgagtgctgc aatatgcaag atctcaaaga
     2041 tatttgctgt cttcacaatg attaaagaaa acttcccatc atccaatttg gcatctggcg
     2101 cgatctgttc gaatcctcca acagagttcg tcagccctag gaaaaacatc gaagcatttc
     2161 cgatatattc cccttcatca tacttcaagc gcattttgat tggctttact cgagggagca
     2221 tctctgctcc tttcgcaaga taagcaagat aaccaaaaat actctttagt tctgatggaa
     2281 cttcataagt caattctgtc aaatagccac ctgcagcgat attgatgaag taactgttat
     2341 cagaaaggcc gatgtccatt tttacggttt ggtgtttttt tatcacttct gctgctgctt
     2401 taatattatc tctaggaatt tttaatgctc gagcatagtc gttcgttgta ccagctggga
     2461 tgatggccat tttaggacgg tgttcaagcg gagcgattcc attaaccact tcgttgattg
     2521 tgccatcgcc accagctgcc actactagat caaaacctag ttctgcgact ctacatgctt
     2581 cattcttggc tgaatctggt tctggtgtag ttgcataggc gctggcttca aatcctgctt
     2641 cttctaatgc ctgtaggata tccgccaaat tttttttcaa taattctttt cccgaggtgg
     2701 ggttataaat tacgcgtgct tttttcattt tcagcctcta tcgtttcgct aattcatctt
     2761 taagtagctt attcacgaca ccaggatttg cttgtccttt agttgctttc atgatttgac
     2821 cgactaggaa gccaaccgca cgatcttttc catttttgaa atcatcgaca gattgttggt
     2881 tgttatccaa aatttcatta atgattggca gtagtttgtt cggatcagat aattgcaccc
     2941 aaccattttt ttcaactact tcttgggctt ctccgccatt tgtgatcaat tcacggaaga
     3001 cttttttggc gatttttgag ctgatcgttc catcggcgat caaacgaatc attcctgcta
     3061 agttctcagg tgtcaatttt gtctcagcta attccagttt ctcactgttc aagtaagcag
     3121 aaacttcacc catcaaccag ttagatgctt gcttggcatc tgcaccattt tccaaaacag
     3181 cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac cattgcgtca tactctggaa
     3241 gtccaaactc acggatatag cgttcgcgac gagaagctgg catctcaggc aaactagctt
     3301 ttactttttg aatccattcg tcatcgatca cgaatttcgg gatatcaggc tctgggaagt
     3361 aacgataatc actagaccct tctttgacac gcatcaaaac tgttttattc gttgtttcgt
     3421 caaaacggcg tgtttcttgt tggatttctc ctcctgaaag aagaactttt gcttgacgtt
     3481 tttcttcaaa agctaaacct tttttcacga aagataaaga gttcaagttt ttcaattctg
     3541 ctttcgtacc gaactcttct tgtccataag ggcgaagaga aatattggca tcacagcgca
     3601 tagagccttc ttccattttc acatcgctga cttctgtaaa ttggataatt gatcgcaatg
     3661 cttctagata agcgtatgct tcttctggtg aacgcatatc agcttcagaa acgatttcga
     3721 tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata accgccaata ccatgcatgt
     3781 tttttccagc atcttcttct aaatggacac gttcgatccg gatttttttc tttttacctt
     3841 ctacttcgat ttcgatccat ccgtcatgac cgattggttg atcaaattgg gaaatttgat
     3901 atgcttttgg attatctggg taaaagtaat ttttccggtc aaaatgagta tctttagaga
     3961 tttcacagtt taatgccaaa gctgctttca tcccgaattc cagcgcagct ttattcatta
     4021 ctggcagcac gcctggataa ccccagtcga tcacgtttgt attgctgttt ggttctgcac
     4081 caaaatgagc tggcgctgga gagaaaattt ttgagttggt ttttaactcg acgtggactt
     4141 ctagtccaat gactgtttca aaattcattc gtctttccct cctaaaatag ctggttgttg
     4201 tttatggaat tctgttgctt gttcaaacgc atatgccgct tgatacatcg ttttttcatc
     4261 aaaataatta ccgatgatct gcaatccaac tggtaatcct tctgataaac ctgccggtac
     4321 agacatacct ggaagtccag ccaagttgac agggatcgtt aagatgtcac tcatgtacat
     4381 tgtgattgga tcgttaatgt tctcaccgat tccgaatgca actgttggtg aagatggccc
     4441 gatgatcaag tcataatctg caaagacttt ttcaaaatct cgtttgatca atgtacggac
     4501 ttgtcccgct tttttgaagt aagcgtcata ataaccagca cttaaggaga atgtacctaa
     4561 catgatacgg cgttttactt cttcaccgaa tccttctgaa cgtgagttga catatacatc
     4621 atctaatgat ttcacatttt ctgagcgata accgtagcgg ataccatcga atcgttgcaa
     4681 gttggaactt gcttctgatg aggcaatgat ataataaaca gccacaccgt attttgaatg
     4741 aggcaagctg acttcttcca caacagcacc taatgaacgg aatgtttctg ctgctttcaa
     4801 tacagcttct tttacgccag gggccacacc ttcgcctaag tattctttag gtaaagcaat
     4861 cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga
     4921 agaagtaccg tctttttcgt cataaccgct gatagctgtc aatgctaatg cattatcttt
     4981 caccgtacgg gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa
     5041 acgtgagaca cggccataag taggtttcat ccctacgatg ccgttaaaag ctgctggttg
     5101 gcggatactt cccccagtat cactacctaa agaaactgga atttgtccag ctgcaactgc
     5161 tgctgcagaa ccaccagaag agccaccagg tacttttgtt gggtcccaag cattttttgt
     5221 tttcttgaaa taagaagttt ctgtacttcc acccatagca aattcatcca tgtttagctt
     5281 accaacaggg atcatatctg cttgatagat tttatccatg accgttgcat cataaattgg
     5341 ttcaaaattg taaaggatct tagatgcagc ggttgtcaag atatcttttg ttacgatatt
     5401 atccttgata ccaattggaa taccagcaag gacgttgctt tcatcaatcc ctttttcatc
     5461 taaagctttt gcctgttcca atgctttttc ttcgttcaat gtaatgaatg cttcgacctc
     5521 aggctctgtt tctttgattc gttcaaatgt tgcttttgtc aaatcttgta cagtgatttc
     5581 tttcgataca agcatatgat gcagttcttc tagagaacga ttgtgtaatt ctgtcattat
     5641 gcgccagcct ccccattatc gatgattgcg ggtactttga taaagccgtc ttctgtttcc
     5701 ggcacatttt tcaataattc atcgcggttc catccagctt gcgcccgatc ttgtcgcatc
     5761 acgtttactg tttcaaccac attcgacgta aatggaacac cttctgtatc tacttcttcc
     5821 agcatttcaa ccatatcgat gattttacct aattggtcgg taaaacttgc aagttcttca
     5881 tctgcgaacg caagttttgc taatttggca acgtgtttca cttgttcttc actaatcgcc
     5941 atactacccc ttctttctaa acgagttaaa aactttgctc tctattttaa cacaagagtg
     6001 ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac tcatttttca ttcttctact
     6061 gagcgacttt tagccaatgc atctgccatt tgatcttctg tccaaacttc gattcctaat
     6121 tcttgtgctt tggtcagctt gctgccagca tcttctcctg ctaccacgat atccgttttt
     6181 ttcgatacgc taccagtcac tttcccgcca agattttcga tcgtctcttt tgcttcttca
     6241 cgagtaaatc gtgttagttt accagtaagg actactgttt tttctttaaa aggagactcg
     6301 acttcttgca attgggcatt tcgaagccct ttgtattcga aattgacgcc tgcttgtttt
     6361 aattcattca tcaattcatg gacttcttcg ttactgaaat aagtgactac actgtcggca
     6421 atgatatcac cgatcgtatc aagcgcaata atcgactcat agtcactttt actcaatgtt
     6481 tctagatcac cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac atgtcggatt
     6541 cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa
     6601 atattatttg ctgatttttc ttttattttg tccaacgtca ataactcttc ttctgtcaat
     6661 ttgtaaagat cagcaacatc tgctactagt tttttatcat acatctgttc tagtacgcgc
     6721 ggccccaacc catcgatatt catcgcattt cttgaaacaa agtggttcaa accttctttc
     6781 atttgagcag ggcatttagg attgatgcac cgtaaggcta cttcttcatc caaatggaca
     6841 agttcacttc cacaaactgg acaatgtgta ggcaattggt attcttcgct gtctttaggt
     6901 cgtttatcta acacgacttg cgaaacttct gggatgatgt ctcccgcttt ataaatcaga
     6961 actgtatctt tcaagcggat atccttcaac ttaatataat caccgttatg caggctggca
     7021 cggcttactg tcgttcctgc tactcggact ggtgtcatga tagctgttgg cgttactacc
     7081 cctgttcgac cgactgtcca ttcgatgttt tcgattagcg tttcaacttc ttctggcgga
     7141 aacttgtaag ctgctgccca tctcggtgct ttgacagtaa aaccaagttg atcttgtagc
     7201 gagaattcat tcactttgat gacgattcca tcgatttcat agggcaaatc cacacgtttg
     7261 tcatgatatt cttcaatgta tgaccacact tcatcaatcg aatgacatag acgtttttcc
     7321 cgattcgtgt ggaatccaat tttttctagc tcttctagcg catcatactg ggtttttgct
     7381 tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg
     7441 attttagaat ctaactgacg caaagaacca gctgcggcgt tccgaggatt cgcaaagata
     7501 tcttttccct ctgcttctct gtcttggttc aattggacaa atgaccgttt tggcatgaaa
     7561 cattcgcctc ttacttcgat gttcattggt tcttttaatt tgattgggac cgaccggaca
     7621 gtttttaggt tttcagtgat gttttctcct acagttccat ccccgcgagt cgcacctctc
     7681 acgaaatttc catcttcata acgcaaagaa acggaaagtc catctatttt tagttcacag
     7741 caatagtcaa ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc tatcagctct
     7801 tctttgctga aaacgtcgtt caaactgtaa agcggaatat catgagtgac tttttcaaaa
     7861 ccttctaata cttttccgcc tactcgttga gtgggagaat cagacgtgat caaatcagga
     7921 tattgttcct caattgctac taattctgcg tattctttat cataaacata gtcttccact
     7981 gttggtttat cttctacgta gtattcgcga gaccattggt tcagtcttgt acgaagttct
     8041 tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt ccatcattta ccctccacta
     8101 ctaagctttt tcaattggcg caaaggcagc caaaagacgt tttactcctt gacttgggaa
     8161 agccacatct agttctagat ccttcgacgt cccgcttact cggacgactg ttcctagacc
     8221 ccattttttg tgtttgactt tatctccagg tttccagctg ttattttccc ctccagtagc
     8281 acttttagag ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg tttttttagc
     8341 gaatacaggt gtttttttag gcgtaggacg catgccttcg atttccagta actcctgatc
     8401 gatttcttct acaaaacgag aaggtcggtt atattgcgtc cgtccatata atgttctgga
     8461 gaaagcattt gttaaataca acgcttcttc tgctcgcgtg atccccacat atgcgagacg
     8521 gcgttcttct tccagttcgc tttcttccat caatgctcga gataaaggaa aaaccccctc
     8581 ttctagacca atcaggaata caactgggaa ttctaatcct ttggctgcat gcaaagtcat
     8641 caatgtcact tgggaagcat cttcttctaa attgtcaata tcggaaacaa gtgccagatc
     8701 atttaagaag accgttagtt tttcttctgg tgcatcagcg tcttcttcat tttgctgttc
     8761 gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc
     8821 ttgtgcttct aatgtatttt ggatttttaa gtcttctaga tatccgctac gatccaaaac
     8881 ctctttcgtc aattctgtca ctgtcaagta tgggatcatc tgagtgactt cttggatcat
     8941 ctctccaaat gcacctaatt gctgtcctgc ttttccaccg atatttgcca gatccacatt
     9001 ttgcgctgct tcaagcagtg gccattcatg aagagaagca aaactgcgta gtttttcgat
     9061 agatcctggt ccgatgcctc gtttgggcga attaacgata cgctcaaaac tgattgaatc
     9121 ttgaggattt gccagcacat tcaaatacgc taaaatatct ttgatttctt tacgatcgta
     9181 gaacttgtgc cctccaacca ttttataagg aatatttgct ttcaacagag tttcttccat
     9241 tacacgagat tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat tccgatgatt
     9301 gcttcgaatc tcttcttgca tccgatctac gataaaacgg gtttcgtctc gttcattatc
     9361 tgcacggtag taagtgatct tgtttccttc tttgttttct gtccacaagt tttttggttt
     9421 tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg
     9481 atagttttgt tctagcaaaa ttactgcagc atctggataa tctttttcaa aatccaagat
     9541 gttctgcata tctgcaccgc gccagccgta aatactttga tctgcatctc ccacgacgca
     9601 caggttccta aatctacctg ccaataaatt gactagcgta tactgtgcgt gatttgtatc
     9661 ttggtactca tctacatgaa tataatggaa tttgttttga taatatgtga gtgaatcagg
     9721 atgttcttca aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca tacattgatt
     9781 atattgaagc tctttttggt aagcagcata acattttgcc gcaatctctt cgtaataact
     9841 gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt gagagattgt
     9901 tcctaatata cttcgaggat catatttttt cggatcaatg ttcagttctt ttagtatccg
     9961 tttcatcaag gtcagctgtt cagaagaatc gatgattgta aagttccggt tatacccgat
    10021 aaaatcaaca tctcttctta aaatacgcac acacatcgag tgaaaagtcg atacccagac
    10081 atcttcgcct ccagatgcga gaatcgcatt gacacgttct ttcatttctt ttgcagcctt
    10141 attggtaaaa gtaatcgcta ggatgttcca aggattgact tctttttcct caatcaaata
    10201 ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct gcacctgcca ttagcagtaa
    10261 tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt ttaataattc
    10321 agctttggat gacattaaca acatcctttc ttttatcaat cttgttaatt atagcatttt
    10381 cttggctttc attaaactag ctagttcaaa atcatttgtt tctttttatg gatttttcct
    10441 tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa
    10501 aggaaaaatc aagaaattag ataagactca gatatatttt attctaaaag gaatatttgt
    10561 tggactgctg acaggaatcg tcgttagcct gtttcgatta tctatcgaaa agctaagcga
    10621 tttagttcga agtatctatg aaatgagtcg ggaacagccg atttacttaa ttggcctagc
    10681 tatttgctgc atccttgcag catttttcgt gggttatctg gtcaaaaatg aaccggatat
    10741 caagggaagc gggattccac aagtagaggg acagcttcgc ggggaattat caatgaactg
    10801 gttctctgtt ttatggaaaa agttcattgg cggtgtactt tcagttggtg cgggcctttt
    10861 tctaggtaga gaaggacctt ctatacagct tggcgcatcc attggacaag gagctggaca
    10921 attgtttcgg agtccatctt cagaagaaaa gattttaatt tctagcggtg caagtagcgg
    10981 acttgcagca gcattcaatg ctcctatcgc aggattactc tttgttttag aagaaatcca
    11041 tcactccttt tctccacttg tttggctgac atcatttgct tcagcaatca ctgctaactt
    11101 cgtctctctt tactttttcg gcttgagacc tgttttatat ctaggtgacc ttccttcttt
    11161 gccgttgaaa tattatggtt ccttagtgat cttaggagta cttctaggca tcttaggttt
    11221 tatttatcag aaggtcttgc tggctttgcc aaaattttac aaccgattgc ctttaccagc
    11281 ttattttcac gggttcgttc ctttcttgct gattttgcca attggttatt tatggcctag
    11341 tactctaggc ggaggcaatc aaatcgtttt agcttttgga catgcttcct tgccaatcat
    11401 tatgattatc ggtttgtttg ttcttcgatt cgttttttcc atgatttctt acggtggtgg
    11461 tcttccaggg gggattttcc ttccgatatt gacattgggg gctttaatcg gtagctttta
    11521 tggaaacatt atggttgatt tcgggatgga tccaatctac atcaaggatt ttgtcgttat
    11581 tgccatggct ggttatttca cagcgattgg aaaagcaccg ctgacagcaa ttattttagt
    11641 gacagaaatg gttggctctc ttaatcatct gatgccttta ggattggctg ctcttgttgc
    11701 ttatatcgtc aatgaccttc tcggcggtaa tccaatttac gaatcattat tggaacgtct
    11761 acttggaaat actcagaccg cctcactaaa aggtgcaagg acaacttttg attttccagt
    11821 aacggcagag agttctttag acggattaat ggtacgagac ttcaattggc ctaaaaatat
    11881 gctgctaatc tctatacgca gaggaaatca ggaaatcttg actcacggtg atacgatcat
    11941 gaatgtagga gatattttgg ttatcttgac tgatgaagca aacttgccaa gaatcaagca
    12001 ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa tcattttaac gaacatggaa
    12061 aaactatttt acaacaacta ttcatctaca ctacttgatt ctatatgatt ctataaaaaa
    12121 agaagagact gggaaaaacc agcctcttct tttttatttc tatgcttctg ctgctgcagg
    12181 agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag caataaacac
    12241 caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccat gaggagccag
    12301 taatttgatc ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa taataaaact
    12361 tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg tgacaaacga
    12421 tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga atttattctt
    12481 aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag ccataacaga
    12541 agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg taccaaacac
    12601 ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag ctaataatgc
    12661 accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca atgcatcatt
    12721 gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa
    12781 cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt tcttgaataa
    12841 tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg cacctaagaa
    12901 tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca aaccagggcg
    12961 gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac caaatgcagc
    13021 ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat ttgataattg
    13081 atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag cgattcctcc
    13141 accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt aaattctttg
    13201 tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat caccatggaa
    13261 tactggtgca gtaccactaa gggctagatt gatcagctct tctggtttac ggataccatc
    13321 actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta cttttttgtc
    13381 tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat
    13441 tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg cttttttctt
    13501 cagagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg taacggcaac
    13561 aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt gttctgcttt
    13621 ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt cttctggtgt
    13681 atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc
    13741 taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga aaaacagata
    13801 agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg cgaataaaac
    13861 tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat caccaagacc
    13921 tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga tatcggaaat
    13981 ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt tatcagtcgc
    14041 ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca tgattatcct
    14101 ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc aatcaattcg
    14161 cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag
    14221 gcttcgattg gatctttcgt acggctgtaa gccccaataa agccggcaat cattgaatcc
    14281 cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata agttccttgt
    14341 tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg tgccccttca
    14401 gctagcaatt ttttaccata cggaatgatt tcttctacag attggaaggt tgtattgtaa
    14461 agctcagcta attcgtgatt gtttggtttg atcaacaatg gtttcttaac aagtgcattt
    14521 tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg aatgatatca
    14581 atcagttctt gataaaatcc ttttcgcagg ctagccggcg cgcttcctga caatacgacg
    14641 atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc ttcatctgaa
    14701 atagatgggc ccaaaccgtt aatttcagtt tcagtatctg attttagttt gatattgatt
    14761 cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc aagccacttc
    14821 gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg agaatcgatc
    14881 cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa gtcttccttc
    14941 attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc aattgatgga
    15001 ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt tcacggaatt
    15061 cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa
    15121 cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat gtctcttcgg
    15181 aatttttcat ggctaaacgt ttaacagctg cctcttctgg gtccggagtc gtgaatcctt
    15241 gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa ttttttagtt
    15301 gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt ccacctaaaa
    15361 taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta
    15421 ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta
    15481 aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt tgctgttttt
    15541 cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat aattgctctt
    15601 tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct tgtaaatcac
    15661 gtcttatcgt tgattcagaa gcattaaaaa ggtttacaag atcttgtgat ttcacgattt
    15721 tctgctgttc aagcaagtct aggatttttt gtctacgttc ctctgtaagc attttcacga
    15781 tcacctcaat acaaataata gcatattttt tttctactcg caatataaat cagtcaaaaa
    15841 cgttcataaa aattataaat cagtcaaaaa aaaataaaaa accagtcaaa aagactggtt
    15901 ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt
    15961 tttcatataa acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat
    16021 cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa
    16081 gagacagacg gataataatg atgccggaac cattattcga taatagagtt gtttatccat
    16141 tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa
    16201 attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt
    16261 tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt
    16321 ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt
    16381 catctgttac ctctacttct agatattccc taatataata acaataagct tcgattccat
    16441 tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgcgag cgattctcgt
    16501 caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat
    16561 ctccttgttg aatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacag
    16621 gttgcatttg tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt
    16681 caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa
    16741 gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt
    16801 ctcgataaag ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt
    16861 ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt
    16921 cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc
    16981 aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct
    17041 ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat
    17101 aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa
    17161 tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc aataattgat
    17221 tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt
    17281 ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt
    17341 gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat
    17401 cgcttatttc ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata
    17461 atttatcttc gccaatcatt ttctcacctc tttaaaatct ttttataatt taattataac
    17521 caacgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat
    17581 agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga
    17641 taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa
    17701 agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa
    17761 acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga aagagtttga
    17821 ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc tgcccacgct
    17881 tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa
    17941 tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg gatcttatct
    18001 gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg atacatctgg
    18061 atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgaccgc acgtagctgg
    18121 tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc agttttggat
    18181 acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc gatcaaataa
    18241 aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt ctttcgatca
    18301 atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag agctatttct
    18361 ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat ttcagcttga
    18421 tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa
    18481 agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct ctgaatcttt
    18541 atagattact gttggctcac cagacaattc agctgcttct tttgtgataa tcactttttc
    18601 gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt caatgatgga
    18661 acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc
    18721 ttcaggttcg aattcaagtt cggtattatc tagagaaaga agtttttgat attgtttgac
    18781 tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca atttctctaa
    18841 agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt ttagtaaatc
    18901 ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt tttgtgtacc
    18961 aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc catcaaatgc
    19021 accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt gatgtggatg
    19081 tttacgtcct ccctgaggtg gcacgcttgc aacagttcct tctagaattt tcagcaaggc
    19141 ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc ttttacgcgc
    19201 aattttatcg atctcatcaa tatagatgat ccctttttct gcacgttcca cattgtaatc
    19261 agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat aaccagcttc
    19321 tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg ctaatgtttg
    19381 tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc ttttttgcag
    19441 ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt gattgtatac
    19501 ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat tcaatacttc
    19561 aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt cataaaattc
    19621 ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga cccctggacc
    19681 agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc gtacggtctc
    19741 attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa gtatgcaaga
    19801 aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa aagaaaaagt
    19861 gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac aaaaaataag
    19921 gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt ctagaagtag
    19981 cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg ctgtacctgt
    20041 gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt ctgtcaacac
    20101 gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga tttctgcatt
    20161 gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga tcacaagatt
    20221 tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt cttctgtaga
    20281 accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt tgttcaagaa
    20341 ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt caacgatttc
    20401 tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc
    20461 ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg ttgatacttc
    20521 gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg cttttacttc
    20581 atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt gataatcttc
    20641 tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta attgatcttc
    20701 aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc cttttccgcc
    20761 atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat cgccattttc
    20821 agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac gtttgatacg
    20881 tgcatccacg tcagcatctg ttacttcacg gtcttgtttt tcaacaacta gatctttata
    20941 ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc agtcttgacc
    21001 tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt
    21061 gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat ataaagcttc
    21121 ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga atccaggtac
    21181 gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga tttcgtcttg
    21241 cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat
    21301 ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttca tgcatacctt
    21361 taaatagtac actaaccctt tcaaattgta aacatattga atggatagaa tccttgtttt
    21421 tcataacatt tttatctttt tcactgataa aatctaatgg aagaagatca tttgctgcaa
    21481 aattttttct ctttttttta aaaaatcttg tttttcatct tctgtacctt catacatcgt
    21541 ttttcctgta cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt
    21601 atcatagaca ggataaacaa ttgcagattg caacgtgaat tcttcattta acacggaact
    21661 aagcagcgca tcttcattac cgtaacgatc tgaaaataca cataattgct ggtaacttgc
    21721 ctgctgctgc ggctttggca gatcttttgg tatgactttg actaactttt cttcaatcgt
    21781 caaatagaaa acaggatggc attcgttcaa ttgcctaagt gtctccagta attttgccct
    21841 tgctaaaata tggacctctg gcgatacaat aagtttactc gctgtttgga taaactcatg
    21901 ataaggaagc tgttcgatct catcaataga cgcaagctga cgtgcaggtt ccaacgaagg
    21961 aagttcctcc gatttattgt ggagtgcctt gatcttctgc ctctccattt gtccggataa
    22021 ttcttctgca aattggattt tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt
    22081 acttgctatc aattttctag catataaaaa atcttgtgta ctgattaaca agtcaaaata
    22141 aaattcagca aactcctcat tttgaagata ctgtttttca tgcaagagtg cttgcttcaa
    22201 tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca agcaggctat tagcctcaaa
    22261 gctttctttc aactcgtaag ctcgttgaaa taattctttt gcctgctgat attgctggtt
    22321 tgctagtgct tgttgtgcta ctcgcattaa attctcatat ttttctggaa aatcaatcgg
    22381 cttcacgcag tgtcctcctc ttgctcatgt tcagctcatg ttcaatagta gcaaaacaaa
    22441 aaaagcatga caattaaaaa taactgaagc accctctaaa agctagattt tagatctgac
    22501 ttttagaagg tgctttcaac tgttactgct ttttattgct catcagctgt tgacttcctg
    22561 gttgaatgct ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa aatcgcctag
    22621 atgttgcttt ttctgattaa agattgctgg aagataccct aacatcagca aaggaaattt
    22681 tagaatgaac cggcaagcac cttctctaac taaaacggtt gaccagctaa gttcttcttc
    22741 atcaaagctg atgactcgaa taccaaagat cattttccca atcgtttgac catgattgaa
    22801 tttcgtcaat aaaataaaat acgctagata aataagtaag gctgtgaatc cgtagatact
    22861 cagataagaa tctgaaaaat taagccaacc tagatttttg gcaataccaa gtgttaaacg
    22921 cgtaatagct ccgatacaca aaagatcgac caagaaagca aacattcgga taaaaaaacc
    22981 tgcatagaaa tacttaggaa aatcatttgc ttttggcggt tcatttcttg tgtccgtata
    23041 ctttttccat ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa
    23101 agagctcagt accttttggg aaaagttgga gtcaggtcct acagcaggct gctgttgctt
    23161 atctttttct tgttccttat tttcatttga aagctctgac atgcttactc acctccgtaa
    23221 taatacattg cttttggtga ttccgctgtc cctagattct caagaacgga taagatctga
    23281 ctagtctcag aaggcttcaa tccctggaat tctgctagtt tacttcctaa ccatgtacta
    23341 gcaaacccag atggcgtgga agaatattct actagttccg catcttcaag ctgttgttcc
    23401 tttcgcatag cttttaaagc atcttcagga aatcccaatt catcgactaa acctacttct
    23461 tttgcttgga caccatcata aatccttcca tcagcaattt ttttgacttc ttcttcagat
    23521 ttattccgtc cctcgctgac gattgctaca aaacgactat atgcgctgtc gatatatgct
    23581 tgaagcaccg cttcttcttc ttttgtctct ggtcgcgttc ctgaacccat atctttcaac
    23641 gctccgcttt taactgttgt atcttccaca cccagttttt ctagaagtcc tgaataattc
    23701 aatcctgaca tgatcacacc aattgagccg gtcactgttt cttctgttgc aaagatttta
    23761 tctgcttgag cagaaatata gtagccaccg ctagctgcca tgtttttcat gctgacatac
    23821 attgggatat catgttcttt acgaatcgta tccaatttct tagcgatttc cgcactttcg
    23881 tagacaccgc caccaggaga atttacttct aaaaagatac ctttgactgt tggatcttct
    23941 tcaatcgcct tcagttgttc cataaaattt ttatgattgt aaccttcaga agcaaataaa
    24001 cctgaagacc ctcccgcagc aatcgttcca tctactgata attttacgat tttttgcgta
    24061 ctgtctccct cttccaacac atttgaagct agttcttcat ccccatataa ccaactgttc
    24121 aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc
    24181 acaaataatc ctgcagcaat cagaactgct gcccaacgtc tcttattcat tctttctcct
    24241 ttctaataca agttttttat gacgatcaca gtttctgact gctcagaagt gtcgaaccgc
    24301 tctgcttttt cttcccgcgc atacccggaa aacgactctt ttgtcaattc atcttctacc
    24361 gtaaactcac gagcttcagc aacttccaag gcatcaggtg cgatttcttg gatgctatcc
    24421 cagccagtat ctaaaaatat tttcgtatca tctgatacac cagctgatgc ttcgaatttg
    24481 gacaattgtt tttttaacat tcccagtgtc atgatctcaa tttttgaatc catttttact
    24541 cctcctctat ccgattactt tctattgtac cgaactcctg aaaaagatga tagataaaaa
    24601 cccattattt tttgacataa aaagagcatt tgacaaagca tttgatatat accaatttat
    24661 tttttattct ttttttgttg acaagaacat tcgcaaaagg ttatgatttt aaatgtttcc
    24721 agataaaaaa caaaagagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tatctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgctg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaatg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagattctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctctggaaaa attgacacag atccctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagatagccg ccaagttctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gcgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gattggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttactcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccatcatt gatatttaac
    27541 ccgtctggat ttgtgcccat ggtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacctc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgattg
    29221 gcaccccagt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatca gttctctacg
    29461 caaaattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaatgtaat
    29521 gctgacatcc ccagtttctt cagagacaac gatggtaatc gcgtcgctga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttcttttgga atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctct ccagtgatat ctgcatctaa
    29761 tgcaattcct gtctcaatat actcatctaa tccagtatgc cgttcgatag taattagcgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat ctgctcgtat ttttcttgag aaaaacgcat aattttcagg
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttctt tcttctgctt caaggtccgt cgttttttct
    30541 tttctagtct taacgggtac atcgatagat tcctctggtt caagtcgttt cttgattttt
    30601 tctaattctc ccgtcagtgc agcatagttg taaaaacgtg ttttagcgtc ttggatagtc
    30661 ttttcaaata aagcttttgc aagatttgga tttgcgttct ctagcgagga aaagcgaact
    30721 tgtttcctca tgaaatcagg catagcagag aaatcaggtt ttttgtagtc caaagtcatt
    30781 gggttcttgt ttttttcgac cattgcaggg ttatatcgat ataatgacca atagccagaa
    30841 gaaacagctt cttttgcttc tttaagcgat tctttcattc caccaagcaa accatgtgtg
    30901 atacaaggcg tataggcaat gattaaagaa ggtccaggat acttttctgc ttcttcaaaa
    30961 gcctttatcg tctgcatttg attggcacct gaagcaattt gcgccacata aacatttcca
    31021 tatgtcatcg ccatcattcc tagatctttc tttgaagcat actttccact agccgaaaat
    31081 ttagcgattg ccgaagctgg tgttgctttt gaggtttgac cgccagtatt ggaatagact
    31141 tcgttatcca aaacaagaat attcacgtct gctccgctgg caagtacatg atcgatcccg
    31201 ccaaaaccaa tgtcgtaagc ccatccgtct ccaccaatca tccattggct gttttttacg
    31261 aatagatcct ttctcttgta tatatcctcc agttcaggga caattgatcg ttcgctttcg
    31321 atcgcagctt gcagttttgc tgctctttgc tgcgttcctt cgctctcaaa catgtgtacc
    31381 aaccagtctt ccatcaattc tcgtagttct tctgatactt ttggcaatac ttctttgatt
    31441 gaacttgcca aagcctcgcg acgagtctgt gccgctagaa gcataccata accaaattca
    31501 gcattatctt ccaataaaga atttgaccat gcaggccctt gaccacattc attggtcgta
    31561 taaggagaag caggagaagc accgccccaa atagaagaac agcctgtcgc attcgcgatc
    31621 atcattcgat caccgaacat ttgtgtcaat aattttacat aaggtgtttc gccacagcct
    31681 gagcaagcgc ccgagaattc aagaagcggt tgattgaatt gtgtagacaa gacagtattc
    31741 tttcctttga ctggatttgc tttttgtctt aatgtcattg caaaagccca attcacagct
    31801 tgttctttct gttcttcata aggcttcatc actaaagctt tttcttttgc gggacaagcc
    31861 tctacacaca gaccacaacc tgtacagtct tccactgaaa cttggatacg gtattttacg
    31921 ccgtctgcgc ctcgcatttc tctcacgata aatccttctg gcgcttcttc catttcctct
    31981 tcatctgcta aaaatggacg gatggcagca tgaggacaaa caaaggcaca ttcattacac
    32041 atcgtacagc gatcactaat ccactcaggt acttctaaag cgacgccccg cttttccata
    32101 gaagccgttc ctaaaggcat ttcgccagtt gtcattccat ttgctacaag tgtaccaaca
    32161 gagagttgat ttccttcctg tgcattcact ggttcaagta tttcttgtat ataaccagtg
    32221 ttccttttat tgattttttc aggcatctcc aacgttctcc aatggtctgg gatcgcgact
    32281 tgatgaagca gttcggtcgt ccgatcgatt gcttgtatgt ttttttctac gatagccatt
    32341 gatttatgtc catagctttt cagggcttct tcttttagca gcggaagcac ctcatcaaaa
    32401 ggaatgatgt cagctaattt aaaaaaagcc gtttccattg ccgtattgat ccggcgtccc
    32461 aaaccgactt cttgggccaa tctcatggca ttgatcgtat agaatctaat gttattttca
    32521 gccaagtaac gttttaattt taagggcaga tgcgtttcta attgttcgtc gctccaaaga
    32581 gtattcaata aaaaaatgcc acctggcttc aatcctttga ccaagtcata gctatgcagg
    32641 taagctggtg tgtgacaggc aacaaggtcc gcatgctcga caagatacgc tgaacgtatc
    32701 ggcgtatctc caaaacgaag atgagagaca gtcagaccgc cagatttttt cgaatcataa
    32761 taaaaatagc cttgcgcata tttatcggtg tgatctccaa taatcttgat agctgattta
    32821 tttgctccaa ctgttccgtc agatccaaat ccccaaaact tggcctggaa tgtttgcggt
    32881 tctgtcaaat ccagactttc ctttggttcc aaagattgat aagtcacatc atccacgatc
    32941 cctatggtaa accgtttttt gcgtatgctt gggtcttttt tcaattcatc aaaaactgct
    33001 gagatttggt cgggagtcac atctttagac cccaatccgt agcgaccacc aataacagcc
    33061 gggcgaagtt cagaatcata taacgcactt tgaacgtcta ataagagagg ttctccacct
    33121 gcccctggtt ctttacttcg atcaagaaca gcaattgttt ttactgtatg aggcaacttt
    33181 tgcacgaatg tttccactgg aaaaggacga taaagatgga tattcaaaaa acctgttttc
    33241 cgtccgtttg cattcaagta atcaatcgtt tgttcaatcg tctgtgcaac tgatcccata
    33301 gaaacaatga cttcttccgc atcgtctgct ccgtaataag tcaccaagtc ataagaagtt
    33361 cctcgtaatc ggttgatctc tttcatatat ttttgaacga tttcaggtaa tttatcataa
    33421 taggggttga tcgtttcccg ttgttgaaaa tggatgtctg gattctggtt cgtaccacta
    33481 acagtcggat gattgggatt catccctcgt ttacgaaatg aagctaattt ctcctgattg
    33541 atcagaggag ctaattcttc atattccaaa acttcgattt tttgaatctc gtgactcgta
    33601 cggaatccat caaagaaatt caaaaaaggt acttctcctt caatagaagc aagatgggca
    33661 acaggtgcta aatccatcac ttcttgtaca ctgctttcag ctaacatagc aaatcctgtt
    33721 tgtcttgcag ccatcacgtc tccgtgatct ccgaaaatat tcaacgcatt tgtcgtcact
    33781 gcccgactag caacatggaa aacacttggc agtagttccc cagcaatctt gtacatattc
    33841 ggtatcatca aaagcagccc ttgagaagcg gtatatgtag ttgtcagtgc accagttttc
    33901 aatgatccat ggaccacacc agctgctcct gcttcggatt gcatttctac tacttttact
    33961 ggttgaccga atacattttc ttttcctgct gctgaccatt gatctactaa ttcagccatg
    34021 gtcgaactgg gtgttattgg atatattgca gcaatctcgg taaatgcata agaaatatat
    34081 gcggctgccg tattgccatc cattgttttc atttttcgca tgattcatca tccttgttca
    34141 tattctctct tattatctgt tccattataa cgtaaaacat tccatttttt cttttattta
    34201 taaaaatatt tcacatatgt ctatttgaat tggtcatcta atttttagac aaacagtcta
    34261 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
    34321 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
    34381 atcgaatgct cctaaaaaaa tggtcgaatt catcaaccta tcttgtccgt attgccgaca
    34441 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
    34501 agtgatcaaa ttgttcgata aagaaaaaga aagcttgcag cgaggaaacg tgatgcaccg
    34561 gtatctcaca atcagcgatg gtcaaaaagc gatcaaagag atcaaacaaa tattcgatac
    34621 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
    34681 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
    34741 agcacatatt cgatttgttc cgactgtcat cttgggtaaa gaaatctttg atgaatctat
    34801 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataagg agcgtaggac
    34861 aaaagtaaag aacacttttg tctcacgctc taaacaccta taaacggcgg aagcagaagc
    34921 aactccttct tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa
    34981 tggtgtccaa aagcttgcat ctgccagatt atctcatttc ttatgacaag ctgagcaaat
    35041 tatctccacc aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat
    35101 tttcatacaa aatttctcaa attcccttaa cgagtaatat ttatgtacgg tgattcacac
    35161 tagtatccca atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct
    35221 tttatgtatg ctgttagttg attagatggt cgtttgttga gctaggtcgt ttattttaaa
    35281 caagttgttt ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa
    35341 tgcatacgca tctgtatttg ccactttgat tcgtataaag ctaacaactc ctaactcctc
    35401 tcatttaata gttgctctct tttttattca gatacatttt tgctgatcac agcctagtat
    35461 ttcaatggtt ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa
    35521 tggattaata gatttcgatt attcaaagcg attttctatt ggtggattgt gttcccaact
    35581 aacaagacgg attccaaagt cattatataa ctcttcaaga gttaaccctt tcctgttccc
    35641 aatagatgta tctctcccac gctcgatata aaatatggag tttcgaatgc caagacctgg
    35701 caaatctttc cttgttacag ttacactgct actaacttcc ccatataaaa tccagctcct
    35761 ccaaaatgat aatatatttt tcgaccatca tctaactcat gtaacaataa ttgttgtgcc
    35821 ctcttgtcac tcgccaaatg caaacgccac gtgcgatacc atgccatgct acctgcatct
    35881 gcagaaaatc catcaaataa tgcaacaatt gtatcttcta tcacaatcct ttccccatct
    35941 acttcatatt ctaatctaaa atcaaactcc cctcgggtaa tcgcaggtct tggtggctta
    36001 ggacccaata ttattctact taaaaccgca ttcgttccca gatgaaacac agctataaca
    36061 acaataatga ttaatatttt gtttctattt gataaatttc tcattattcg caaatcacct
    36121 ctacattctc acatactagt tcgtaatact cctttaatat caaaatatct actgatcgta
    36181 ataatattcg ctatgaattc ctactactcg tataagaaag gccaaatcat cagcttttac
    36241 ttgctgatga tttggcttgt ttctatttat cgtaattgtt ttctaatcac caaaattgtt
    36301 aacaatcggt tcagatattt cccaattcac tagccttata ttatattgat ttaacaattc
    36361 ttcttcattt attgtatttt gatcaagcgg accaaattgt tctaataatt ctttgtcatg
    36421 taaaatatcc cttctagtaa tgcctctttc cctttcaatg aaagaagcac taggaaaagt
    36481 atgattataa ccattcaacc tatctcccat gtaataatta gcaggtcctg gaaaataaaa
    36541 tatttgctta ttatcactaa cttctaacag caacactaaa tcttctccgc tggctaaacg
    36601 acttgtccat ctacgatatc tccctatgcc ttcgttcatt ccaaatccat caaattcaac
    36661 tattaacgtg tcttctatca catgtaattc ttcattcaat tcatactcaa gtctaaaagg
    36721 aaattctcca tatgtaatct ctggcaatgg tggtgctgga gttctccaaa taaatacaat
    36781 aactgtgaat agaaaaaatc caacgatcga tataaatgtt atgagacctg ctcgaagtaa
    36841 aacccgatat aataaaaatg ctgtatttcg ctttgctata tctagaaaaa taaaaagtac
    36901 tgcaatcata atgatagtaa aagttgtaaa aaaacgcatt tttgccctcc ctttataaac
    36961 atcgattctc aaattctaaa ccaaatcttg gtagttcaag ttcaggtata acgtattcca
    37021 accatacatc tcaattacta tttatgaact tatgattatc actgaataaa tcactccatc
    37081 cccttgttaa aacttctaca ttaattacac ctggttgttt tcgactagct aatccaatga
    37141 acttatcaac catccttatc caccaaccac gagaatcttg accagaagta agaagagcag
    37201 ttctcaagga tgatcgagca gttccttcca gttttttaat taatcaggcc agttatagat
    37261 aaatacttga tcttatctat atcaggactt ctatattaag caaaaattta tgtacaaaac
    37321 taaaatcttc attcagttgt ctccccgctt tttatacgat tgtattaaaa acaatagaag
    37381 cctttccaaa ttatgttact ccagtaaaga tggaattata gtttgacgga agaaaagcaa
    37441 gtataacttt taaaaaaaac tgatacattc gcatctcctg cttcatgtat cagttttttc
    37501 tttggattaa actgcttctt cacgcagttt ttccgcaaat tcatttattt tgcgaattcg
    37561 gtgattaatt cccgattttg atatcgcacc tgagggaatc atttccccta attctttcaa
    37621 gctgacctct ggatgttgaa gccgaagttc tgcgatttcc tgaagttttt caggaagggc
    37681 atgtagaccg acctttgctt cgatcaattc gatgttttcg atttgtttag aagccgcatc
    37741 aatcgtcttg ttcagatttg ctgtctcaca attgactaga cgattgaccg aatttctcat
    37801 atcccgtacg attcgaacat cttcaaatcg gagcattgag ttggttgcac caatcaatgt
    37861 taagaaatca gcaatttttt ctgctccttt taaataagaa atatagccat tgcgccgttc
    37921 aagcgttcga gcatgtagac cgtaataatt taacatatcg caaatgtctt ggttatgctg
    37981 ttcataaata gaaaatattt ccaaatgata acggctcgtt tccggattat tgactgaacc
    38041 tgaggccata aaagcaccac gtaagtaaga acgcattttt tgcgaatttc ccataatctc
    38101 gtccgaaacg tgtccattga acatcatacc atccatgata tcaagatcca tcaacacatc
    38161 ttttgtgcct tgcttcaacc ggacgatata gacattgttc ttttttaatt tcattttttt
    38221 acgaacgagt agctcacttc gtacctgata atgatctttc aaaagagaat agatacgtct
    38281 ggcaattgca gcattttctg tttggacgtt caaaataaac tgctggttca caatacctaa
    38341 agagccgttc attcgcagca aagcagctaa ttccgctttc gcatgttctc gatgaacttc
    38401 caaaccagtt agttctttct tcacatctgc agcaaatgac atcctattct cctcctttct
    38461 ccaacaataa attgcttaat attttgaacc gaaaactaaa cggaacaatt cttctactac
    38521 tttgtctcca tcgtggaaga caccaccatc tttcaattcc aaaaagtcgg tggaaatcac
    38581 tcgacatcct tcatttctca atccgttgaa gtcgtgtgcg acttgtacta gatactcatc
    38641 atagatctct gggtccatat aatcttcagg cactttttcc gtattgacca atactgtatc
    38701 gataaaaggc tgattcaaat gcttgtgtaa aacacgtata tgatcggcat ctgtgaaatg
    38761 ttccgtttcc cctttttggg tcatgatatt acagatatag acggtttcag cagttgtctt
    38821 gagcattgct tcgccgattt ctggtatcac taaatttgga aggatactgg tgaataaact
    38881 tccgggtccc aaaacgacca tgtcggcgtc cataattgct gaaacgactt ttcgagccgc
    38941 tttggcttca cttttatcat ttgtgttgca gacatacaca cggtcaatgg ttttacggtc
    39001 gatcgcgatt ttagattctc ctactgcagt cgttccgtct ttaaagacag catgcaaggt
    39061 caaaggagtt tctgaagaag gataaacatg tccatctaca tgcatcattt ttgctaataa
    39121 ttgaatcgcc tcataagtgc ttccccgcat ctcggaaact gcggcaatga tcagattacc
    39181 gatcgtatga ttggctaaaa actgatcttc ctttttgaaa cggtattgga aaatatcttc
    39241 atagaattgt ggcatatccg ataacgccac taagacattt ctcaagtcac caggaggagc
    39301 catagcgatc gattctctga ttgcaccgct gcttccccca tcatctgcaa cagtcactac
    39361 tgctgtgata tctgctcctt gatttcgcaa acttttcagg ataactggca gaccagtgcc
    39421 accgccgacc actacgatct ttggttttct tatccggtag gttttcattt tcacgaccgg
    39481 ttcaccgtct ctttcctttt atccttgtcc cgatgtgtca cattgacttt atagtttttc
    39541 cctaaagcat ttgccaaacg ttcagtcaat gccacagaac ggtgttgacc gcctgtacag
    39601 ccgattgcaa ctgtcaagct gctttttcct tctttaatgt atccaggcaa tacagtttcc
    39661 agaagatcca aaaatttatg gtagaatgat tgcgtttcat caaaggacat cacataatca
    39721 tagactggct gatcttttcc ggtcaatggt cttagttccg ggatataatg agggttgggc
    39781 aaaaaacgca catccatcac aatatccgaa tcgatcggca acccgtattt aaaaccaaat
    39841 gatacaaatt cgatccgaaa tccttggtct ccctttgatt tgaatgactc attgattttt
    39901 tcacgtaatt gtcgtggtgt caatgtcgtc gtatcaatga tcacggaagc ttgggctttc
    39961 aagtcgtcta gtatcgcccg ttctttacgt atcccttcag tgatcaaacc gtccatcgcc
    40021 attggatgtg ctcgtcttgt ttctttgtag cgagccacta gttcttcgtc tgaagcatct
    40081 aaaaataaga tagaagtgtc gatgaagttc gtattttcga tttcgatcag catgttttgg
    40141 atttcttcaa agaaggaacg agaacgtaaa tccactccta aagccatttt tgtgactttg
    40201 cctgattctt tgattaattc ccagaatttt ggaatcaatc gaggcggcat attatcgata
    40261 caaaaatagc ccatatcttc aaaactttgg atagctactg ttttccctgc accgctcata
    40321 cctgttatga tgactaattg caaattatct gtcataatta cgaactcctc tcacattgtg
    40381 catagtaaaa ttatatcatt ccgggcgtat cattaccagt tttctttttc ttttacaaaa
    40441 agatttggac caaatatctt ttaggatatt tgccccaaat ccaatttttt tacttttccg
    40501 atgctgtatc caatgccttg cttctttcag ctagcgctcg ttcaatcgtc cgatctgcta
    40561 aaatagattg ggcatcgatc actggataat ggttatcttc agcaaattca tgcatcaatg
    40621 acaattctgt gcaacctaag ataacttttt cacagtttag gcgctctacc gcttcttcta
    40681 agatttcgta atatagctct tgattcaaat aatccgattc cttgatctca tgatagatca
    40741 gataattgat tttttcttga agaacagtat caggaatcac tgtttcgaat ccaagatttt
    40801 tgacttcgcg ttcatagatt ccagctttca tacttccttc tgttcctaga atcgctactc
    40861 tccctgtcgt atgctgacgg acaagttcat ttgctgcttc tcttggcata tgaaggatag
    40921 gaatatccgt tgccgcttgt aattcttcaa aaaagtagtg tgcggtgtta caagtcaaca
    40981 caatgaagtt cggctgcaat aaattttgtt tctcaatatc atccagcaga aacggcattg
    41041 gattttcttc tgatcgatct aaaatataag ctgtacgatc aggaactgtt gcatgattga
    41101 ataagacata attcaaatat tcttgatctt ttgtagcttt cgttcggtgg ttgatcaagc
    41161 gaacaaaact ttctgtcgcc atcgtgccca ttccgcctaa aatactgaaa aaattctcca
    41221 tgaactagcc ttctttcaca tgaaaatact ttttgaatct tggaatatag ttatggaaca
    41281 tatatttcat caacaaccag cgcttgattg accggtcttt ttcataaaac acagtagtac
    41341 cgtaacgatt ttctttgatc atttgctcag ctagtttttt gtcttcattt tcccgagcat
    41401 attctaaaaa gatttttttg ggcacaccca tccataatct tgctttatct gattgattcg
    41461 ttccataggt cgtttcaaca aagggtttgt taaacactcg atcttctgtg acgaaacgag
    41521 ccagattcaa gccgttcaat gtcacaaaga aactgctacg accttgtctc aaattgattt
    41581 caaataattt atattctcca tctctaggat catacttcat atcgaaattg gcaaagcctg
    41641 tatattcgat tttttccaaa aatgccttaa tggtttgata gattttttcg ttataatctg
    41701 gcatgatcac aacgtaatta ccaatcgatg ctggtgtagg atcttctaac agtggatgac
    41761 caagacacat catccgaact tgatgatctt catctacata cgcatttagc acacgcatat
    41821 tactatcatc acccgggata aaatcttgta caatcatttc acttttataa ccggcttcgt
    41881 atatccggcc taaaattaaa tcgaactctt ctctagtctc taaaataaaa gctttttttc
    41941 ttccttcaaa ttggacagaa agatactcca cactgtttgc aggtttcaaa gcaactggga
    42001 attcaaaagg caattcctgt tccaaatgtc catttactaa catttcttct ctgacgatca
    42061 atgttttagg atacggaaga tcatattctt cacaaacttc ataaaagctt actttattga
    42121 tcagtctttc aaataaagaa taatcaatat atggacagat aaaatattct gacagctctt
    42181 gtttatgttg ggagatcaat tctgcatatc catcaccaca agcaatcaaa agatattttt
    42241 tactcttatc agaatatctt tcttttgcca aacgaagcat cgtctcgata aacacagggt
    42301 ctttatcaaa tcctggaata acttctacat tgacaatttt actgtagcgg gtaggggcta
    42361 actgatccga agcatatgct tggcagattt tgccgtatgc ttcattaaaa gaacgtgcca
    42421 taccataaac gttcatgtcg cttcctaata atatagggat aaattcttca ttttcaatac
    42481 tgttcatcat tatctctcac ttactcataa tctcttgttt ttcttttcca actacacaag
    42541 gaatatcata ccataaagaa aaaagcaatc aaaggaaact cctccgattg ctcaattctt
    42601 ttttgaacgg attgaactta taggaagttt ttgtctatac caatcagtgt agaaagaact
    42661 accatgtaat gattctagaa cactgatatt aataaaacaa gcaaaaattc atttttttat
    42721 gtttttttaa ctatctcagc tatctatcct ctttatacca ttattttttt caaatagtga
    42781 cctgtataac tttcttttac gttaatgatt tcttctggtg ttccagtcgc aaggatcgtt
    42841 cctccacctt ctccaccttc tggtcctaaa tcaatgatat aatctgctgt tttgattaca
    42901 tctagattgt gttcaatcac taaaactgtg ttaccagcat ctactaatct ttgtaataca
    42961 tgcaacaagc gggcgatgtc atcgctatga agtcctgtcg ttggttcatc tagtatatag
    43021 aaattctttc cattagagat tttgtgcaat tcactggcaa gtttcatccg ttgtgcctca
    43081 ccaccggaca atgtcgttgc tggttgcccc atcgtcacat aacctaagcc aacatcaaca
    43141 atcgtttgca gtttgcgatg aatctttgga atgtgcttga agaattctac agcatcttct
    43201 actgtcattt ccaaaatatc agcaatgctt tttcctttgt aatgcacttc taatgtttca
    43261 gagttatatc gtttgccatg acatacttca caaggaacat agacatcagg caaaaagtgc
    43321 atttcgatct taataattcc atccccgcga caagcttcac aacgaccgcc ttttacgttg
    43381 aaactaaaac gccctttctt ataaccccgc atttttgcct cgttcgtttg agcaaataac
    43441 ccacggatat catcaaatac acttgtataa gtcgctggat tacttctcgg cgtccggccg
    43501 attgggcttt gatcgatatc gatgatcttt tcgatacttt cgtagccgga aattgtcttg
    43561 aatttacctg gcttagcaga attcttattt aatttttgcg ctaacgattt ctttaagata
    43621 ctattgacca atgtactctt tccagaacca gacacacctg tgactgctac aaattctcct
    43681 aatggaaatt caacactaat atttttcaga ttgttttctg aagcaccagt gatcttgatt
    43741 gctttcccat tacctttacg gcgttctttt ggtacaggga ttgatttttt ccctgaaaga
    43801 tatttccctg tcaaagaatt ttcatcatta gctacttctt caggagtgcc tgcagccacg
    43861 atttgtccgc cttgttctcc ggccccaggt ccgacgtcaa tcagataatc tgctgcccac
    43921 atcgtatctt catcatgttc gactacaacg agggtgttgc caagatcacg cattttcttt
    43981 aatgagccaa tcagtcgatc gttgtcgcgt tgatgcaatc cgatcgatgg ttcatctaaa
    44041 atatacaaaa ctccagaaag attcgatcca atttgtgtag ccaatctgat tcgctgcgct
    44101 tctcccccag ataatgttcc ggaagcgcga ctcaaagtta gatagtctag tcccacattt
    44161 tccaagaatg aaagtctgtc atttacttct tttaaaatcg gtttggcaat tactttctct
    44221 tgttcagata acgttaagtt tgagaagaaa tctactgctt tttgaataga caactcattt
    44281 gtttgcccga tattcgttcc attgatttga acagatagag cttggggatt caatcgataa
    44341 cctttacatg tctgacaagt taattctgtc atataaagac gcatttgctc acgcgtgaaa
    44401 tcactgttcg tatcatgata acgacgctcg atattcttca ataccccttc gaaagtcgtt
    44461 tctacatcgc gaacgccacc aaaatcattt tcataatgga aatggaaaag atcatctgtc
    44521 ccattcaaga tgatatcctg atgctcttcc ggtaattctt cgaatggtgt atctaaatcg
    44581 ataccgaaac tttcacaagc ctgcgctaac atttgaggat aatactgaga gctgattgga
    44641 ttccatggaa cgatagcacc ttgactaagt gtcttcgttc tatctggaat cactaaatct
    44701 tgatcgactt ctaatttgac acctaaacca tcacagtctg gacaagctcc aaaaggcgca
    44761 ttgaatgaga acagtcttgg ttctagttcg ccaacagtaa atccgcaata aggacaggca
    44821 tagtgttcgc taaacaacat ttcttcttca ccgatcacgt caaccagagc atagccgtct
    44881 gccaatcgca gagccgcctc taaagaatca aatagacgag aacgaatccc ttctttcaca
    44941 atgatccggt cgatgacgat cgcgatatca tgttttttgt ttttctccag ctcaggcact
    45001 tcacttaaat catagatctc accatcgact cgaacgcgga cgtatccctc tttttggatt
    45061 cgttcgaata tttttttatg ttgtcctttt ttctttacca cgatcggtgc caacacttgg
    45121 atctttgtgc gctcaggcaa agccattact tgatctgcca tttgatctac tgattgactg
    45181 gtaatctcga tatgatcgtt gggacaaatc ggatgtccta cacgtgcaaa caacaagcgt
    45241 aaatagtcat tgatttccgt cacagtacct actgtcgacc gaggattttt acttgttgtt
    45301 ttctgatcaa tggaaatcgc cggactcaat ccgtcgatgc tatctacgtc cggcttatcc
    45361 atttgtccta aaaactgacg agcataagca gaaaggctct ctacgtatct ccgctgccct
    45421 tcagcgtaca gcgtatcaaa tgctagggaa ctttttccag aaccggacaa acctgtaacg
    45481 accacgaatt tatctcgtgg aatcgttaca tctatatttt tcaagttatg ggcacgtgca
    45541 ccgtgaatcg taattttatc gttagccatg ttgtcctcct actttgaagc ttttaattct
    45601 aaaatcatat ctcgcacatt agcggctgtt tcaaaatcca atgctttcgc cgcatttttc
    45661 atttcttttt ctaatttcat caataacgta tctttttctt ctttcgtcat ttcttcatac
    45721 gagacttgta ccgcttcttt tgtatcatct tccgattctt tcgtaataga aatcaaatct
    45781 cggatttctt taatgatcgt ttttggaacg atcccatgtt cttcattgta cttctgctga
    45841 atcgtacggc gtcttgatgt ttcatccatc gctagacgca tagaatctgt tactttgtct
    45901 gcgtacatga ttactttacc ttcttcattc cgagcagctc gtccaatcgt ttggactaag
    45961 gaacgttcac tacgtaaaaa gccttcttta tccgcatcca agatagcaac aagggaaact
    46021 tcaggtacgt ctaatccttc acgaagcaaa ttgattccaa ctaacacatc aaattcacct
    46081 aatcgaagat ctcgaatgat ttctgttcgt tccagcgttt tgatatcact atgcaaatat
    46141 ttgactttga tccctaattc tttgaaataa tctgtgaggt cttctgccat ttttttcgtc
    46201 aatgtcgtta caaacacccg ctggtctttt tcgactcgtt catttatttc gcctactaga
    46261 tcatcgattt gtcccatgat tggacgaact tcgatcactg ggtcaagcag ccctgtcgga
    46321 cggatgattt gttggataac ggtatctgtt tgttcatgtt catatggtcc tggcgtcgca
    46381 gagacataaa tgatctgatt gacatgtttt tcaaactctt ctaaccgaag tggccgattg
    46441 tctaaagcag atggcaaacg gaaaccgtaa tctactagca tctgttttct cgctcggtca
    46501 ccattataca ttcctctgac ttgaggcatg gttacgtgcg attcgtccgc cacgatcaaa
    46561 aaatcttcag gaaagaaatc caatagcgta taaggcggtt caccttcttt acgtccgtcc
    46621 atatgccgag aataattttc aattcccgac gtgtagccca tttctaacat catttcaata
    46681 tcgtaattgg ttcgctgttc taaacgttga gcttccagaa gtttgttttc atttcgcaag
    46741 acagttaaac gctgttctaa ttccgctttg atattcgcta cggcatgctc catatgttca
    46801 tcattcgtca caaagtgagt cgccgggaag attgccacgt gttccgtttc tcctaaaact
    46861 tcacctgtca gtgcattgac ttctcggata cgttcgacct catctccaaa aaactccaca
    46921 cgtaaggcac gttcatctct tgaagccgga aaaatctcca cgacatcacc gcgcactcga
    46981 aaacgaccgc gctgaaaatc tatatcattt ctttcgaatt ggatactgac taagtcacga
    47041 atcaactgat tgcgatctag ttctgctccc tgtcgaatcg aaactacttg tttttggtac
    47101 tcaaaaggcg aacctaatcc aaaaatacat gaaacggatg caatgacgat cacatcattt
    47161 ctttctaata atgaactagt agccgaatgt cgtaatttgt cgatctcatc atttacactt
    47221 gaatcttttt cgatatacgt atcgcttgaa ggcacataag cttcaggctg ataataatca
    47281 taataagaaa caaaatattc aacagcattg ttagggaaaa attccttgaa ttccccgtac
    47341 agctgaccag ccaatgtttt gttatgggca ataattagtg ttggtttatt cactttctca
    47401 ataagattgg aaatcgtata tgttttccct gttccagtcg cacctaataa gatttgtgct
    47461 tttttcccgc cgactacgcc atccactaat tggttaatcg cttcaggctg atcaccagcc
    47521 ggttggtatt tcgagacaag ctcaaagtgt cgggaggtat ctctctcaat cattcttcgt
    47581 cctccttttc attcattcct tttcatttta cgaccaataa aaagaaaagg aaagcaaagg
    47641 cattccttac taaaaaatta taccacaccg aacatacttt cgccactttt ttatcttatt
    47701 atgcatgtta cgttttataa cataaaatgt tagaaactcg catttttttc attttttcga
    47761 gcttacagtc ttgtctgatt cagcactttt cgcctataat aataaaatat tggaaattta
    47821 ggaggttgat tatgaaaaga aagcatttat ttcactcatt tgttttctta atgacaggac
    47881 ttgtgacact tgcgtttgga catgttgcac aagctgacaa cactacaacg gcagagtcaa
    47941 gcacaagcga gacaatgcaa aaaatagagc ccaaaaaaga cgtatacgta attgccagtg
    48001 actctgcttt tgctccattt gaatttcaaa acgcaaaggg cgagtacgaa ggcatcgatg
    48061 taaaattgat ggaaaacatc gccaaactgc aaggatttaa catccgaatg gactaccgtg
    48121 gtttctctgc tgctttgcaa gctttggaat caggacaagc agacggaatg attgccggaa
    48181 tgactgtaac agatgaacga aagaaaagct atgatttttc tgattcttac tttgatagcg
    48241 ggatccaatt agctgtcaaa aaaggagata cttcgatcaa gtcttatgaa gatttgaaag
    48301 gaaaaacagt cggagcaaaa gttggtaccg aaagtgctga atttttaagc gaccatgaat
    48361 ctgaatatgg ctattctatc aaatattttg atgctgctga tcaattgtac gacgggttaa
    48421 aagttggcgc aatcgatgca atgatggatg attatccggt aatcggttat gcgatcaaac
    48481 aaggacaaga attggaaaca ccgatcaaaa aagaatctgg cggaaaatat gcatttgccg
    48541 ttaaaaaagg acaaaatcct gagcttctta atatgttcaa tgaaggattg aaagaattaa
    48601 aaagaacagg cgaatacgat aagattatta aagaatatgt agcggatggt tcagaaactt
    48661 ccagtgagtc ttccactgct gatgaatcaa catttcttgg acttatccaa aacaactatc
    48721 aagcattgtt gaaaggactt tggagaacaa ttctcctaac tttgatctcc tttgctttgg
    48781 ctatagtaat cggtgtgatt tttggattat tcagtgtagc accaatacgc gctcttcgca
    48841 caattgctag tatttatgtc gatatcatcc gaggaattcc aatgatggtt cttgcgttct
    48901 ttattttctt tgggttgcct ggtatcgttg gatttacgat tcctgacttc cttgctggtg
    48961 ttatcacctt gactcttaac gcaagtgctt atatcgcaga gattgttcga ggcgggatca
    49021 acgccgttcc tgtcggacaa atggaagctt ccagaagttt aggtctttct tataatcgga
    49081 caatgcaaaa aatcattttg ccgcaagcga tacgcatcat gatcccatca ttcgtcaatc
    49141 agttcgttat ctcattaaaa gacacaacga tcatttccgc aatcggtgta gtcgaattgc
    49201 tgcaaactgg taagatcatt gttgcacgta caacacaaag tacgtatgtt tacttgatca
    49261 ttgcgatcat gtacttgatt ttgatcacag cattgacaaa attagcaaaa gttttagaaa
    49321 agaaggtgaa gtaagatggc agaaaaaatc ttagttgagc atttagtcaa aaaatacggc
    49381 gataacacag tattaaacga catcaatgtg tccatcaacg aaggagatgt cgtctgtatc
    49441 attggccctt caggttcagg aaaaagtaca tttttgcgtt gcttgaatcg tttagaagaa
    49501 ccttctgacg gtgaaattat tattgacggc gcccacttga tggataaaag cacagatatc
    49561 aacttggtga gacaacatat cggaatggta ttccagcact ttaacctatt ccctcatctt
    49621 tctattttgg aaaacatcat tttagcaccg atggacctga agaaagaatc tagagaagaa
    49681 gcagagaaaa aagcaatcaa attgctgcag acagttggtc tagaagataa aaaagatatg
    49741 tatcctgaaa tgctttccgg tggacaaaag cagcgtgtgg ctattgctcg tgctctagcg
    49801 atgaaccctg acatcatgct ttttgatgag cctacatctg cactcgaccc cgaaatggtc
    49861 ggtgatgtct tgaacgtcat gaaaaagtta gcagaacaag gtatgacgat ggttatcgtt
    49921 acgcacgaaa tgggcttcgc taaagaagtg gccaatcgtg tcatgttcat tgacgacgga
    49981 aactttttag aagacggaac gcctcaacaa atctttgaaa atccgcaaaa tgaacggaca
    50041 aaagatttct tgaataaagt attaaatatt taataaaaac caaaaatgcc cggtcgcttc
    50101 atgacgaccg ggcattttta gtttcttgtt ttttcactta actgtaccag ccgcatcgct
    50161 tgttctcctg cacaaacaag caatcgctct acctgttcca tcgcataaga aagtaccatc
    50221 ggttcattga cgatatcaag tacatagtcg ataccacctt cagaaactat gatgattatc
    50281 ttattctaaa gctggctttt ttgttggtac aaacatgaca actacaaaaa aaccaggcga
    50341 acgtatgatt
//
LOCUS       DASHFF010000015        49814 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.015, whole genome shotgun sequence.
ACCESSION   DASHFF010000015 DASHFF010000000
VERSION     DASHFF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.015"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            598..1287
                     /locus_tag="U2I75_000975"
     CDS             598..1287
                     /locus_tag="U2I75_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1696125.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="U2I75_000976"
     CDS             1274..2482
                     /locus_tag="U2I75_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1696126.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="U2I75_000977"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="U2I75_000977"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN1696127.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="U2I75_000978"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="U2I75_000978"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN1696128.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="U2I75_000979"
     CDS             complement(6585..7280)
                     /locus_tag="U2I75_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN1696129.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="U2I75_000980"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="U2I75_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN1696130.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="U2I75_000981"
     CDS             complement(8479..8670)
                     /locus_tag="U2I75_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN1696131.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="U2I75_000982"
     CDS             complement(8691..9581)
                     /locus_tag="U2I75_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN1696132.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="U2I75_000983"
     CDS             complement(9568..10335)
                     /locus_tag="U2I75_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1696133.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="U2I75_000984"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="U2I75_000984"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN1696134.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="U2I75_000985"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="U2I75_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN1696135.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="U2I75_000986"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="U2I75_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN1696136.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14906..15289
                     /locus_tag="U2I75_000987"
     CDS             14906..15289
                     /locus_tag="U2I75_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696137.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="U2I75_000988"
     CDS             complement(15457..16215)
                     /locus_tag="U2I75_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN1696138.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="U2I75_000989"
     CDS             complement(16233..17051)
                     /locus_tag="U2I75_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1696139.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="U2I75_000990"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="U2I75_000990"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN1696140.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="U2I75_000991"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="U2I75_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN1696141.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="U2I75_000992"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="U2I75_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN1696142.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="U2I75_000993"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="U2I75_000993"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN1696143.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21070..21312
                     /gene="yaaA"
                     /locus_tag="U2I75_000994"
     CDS             21070..21312
                     /gene="yaaA"
                     /locus_tag="U2I75_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN1696144.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21299..22423
                     /gene="recF"
                     /locus_tag="U2I75_000995"
     CDS             21299..22423
                     /gene="recF"
                     /locus_tag="U2I75_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN1696145.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22420..24366
                     /gene="gyrB"
                     /locus_tag="U2I75_000996"
     CDS             22420..24366
                     /gene="gyrB"
                     /locus_tag="U2I75_000996"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN1696146.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24386..26857
                     /gene="gyrA"
                     /locus_tag="U2I75_000997"
     CDS             24386..26857
                     /gene="gyrA"
                     /locus_tag="U2I75_000997"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN1696147.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27093..27392
                     /locus_tag="U2I75_000998"
     CDS             27093..27392
                     /locus_tag="U2I75_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN1696148.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27442..27954
                     /gene="ssb"
                     /locus_tag="U2I75_000999"
     CDS             27442..27954
                     /gene="ssb"
                     /locus_tag="U2I75_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1696149.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27980..28216
                     /locus_tag="U2I75_001000"
     CDS             27980..28216
                     /locus_tag="U2I75_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN1696150.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28359..30332
                     /locus_tag="U2I75_001001"
     CDS             28359..30332
                     /locus_tag="U2I75_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN1696151.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30338..30790
                     /gene="rplI"
                     /locus_tag="U2I75_001002"
     CDS             30338..30790
                     /gene="rplI"
                     /locus_tag="U2I75_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN1696152.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31064..32431
                     /gene="dnaB"
                     /locus_tag="U2I75_001003"
     CDS             31064..32431
                     /gene="dnaB"
                     /locus_tag="U2I75_001003"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN1696153.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33211..34503
                     /locus_tag="U2I75_001004"
     CDS             33211..34503
                     /locus_tag="U2I75_001004"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN1696154.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35239..36102
                     /locus_tag="U2I75_001005"
     CDS             35239..36102
                     /locus_tag="U2I75_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1696155.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36231..37853)
                     /locus_tag="U2I75_001006"
     CDS             complement(36231..37853)
                     /locus_tag="U2I75_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1696156.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38102..38623
                     /locus_tag="U2I75_001007"
     CDS             38102..38623
                     /locus_tag="U2I75_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1696157.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38809..40539)
                     /gene="spxB"
                     /locus_tag="U2I75_001008"
     CDS             complement(38809..40539)
                     /gene="spxB"
                     /locus_tag="U2I75_001008"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN1696158.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40704..41522
                     /locus_tag="U2I75_001009"
     CDS             40704..41522
                     /locus_tag="U2I75_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN1696159.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41625..41822)
                     /locus_tag="U2I75_001010"
     CDS             complement(41625..41822)
                     /locus_tag="U2I75_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696160.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41991..42569
                     /locus_tag="U2I75_001011"
     CDS             41991..42569
                     /locus_tag="U2I75_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1696161.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42664..42801
                     /locus_tag="U2I75_001012"
     CDS             42664..42801
                     /locus_tag="U2I75_001012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696162.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43614..44117)
                     /locus_tag="U2I75_001013"
     CDS             complement(43614..44117)
                     /locus_tag="U2I75_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1696163.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44476..45501
                     /locus_tag="U2I75_001014"
     CDS             44476..45501
                     /locus_tag="U2I75_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1696164.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45824..46702
                     /locus_tag="U2I75_001015"
     CDS             45824..46702
                     /locus_tag="U2I75_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEN1696165.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47178..47294
                     /locus_tag="U2I75_001016"
     CDS             47178..47294
                     /locus_tag="U2I75_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1696166.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48222..48560
                     /locus_tag="U2I75_001017"
     CDS             48222..48560
                     /locus_tag="U2I75_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696167.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48654..48884
                     /locus_tag="U2I75_001018"
     CDS             48654..48884
                     /locus_tag="U2I75_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696168.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49284..49655
                     /locus_tag="U2I75_001019"
     CDS             49284..49655
                     /locus_tag="U2I75_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN1696169.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT        15403 a         9237 c         9737 g        15437 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtat attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt
    20941 atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata
    21001 aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag
    21061 ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg
    21121 ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac
    21181 actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg
    21241 atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat
    21301 gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt
    21361 ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga
    21421 aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat
    21481 tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct
    21541 tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca
    21601 gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct
    21661 ttctttagta aaaggcagcc cgcagaccag gcggaagttt ttagatatgg aattaggcca
    21721 gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa
    21781 ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt
    21841 gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa
    21901 gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct
    21961 ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca
    22021 agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac
    22081 gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac
    22141 ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga
    22201 tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga
    22261 gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt
    22321 cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt
    22381 ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta
    22441 gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc
    22501 gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt
    22561 gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc
    22621 catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct
    22681 gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat
    22741 gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga
    22801 tcttctgttg ttaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat
    22861 gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa
    22921 acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca
    22981 actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc
    23041 gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat
    23101 tatgacggcg ggattaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt
    23161 ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa
    23221 tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa
    23281 ggcggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc
    23341 cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa
    23401 ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa
    23461 acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc
    23521 atgaaattct tgctggaaaa cccaagtgtg ggccgtcaaa ttgttgaaaa aggattacta
    23581 gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct
    23641 ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa
    23701 tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat
    23761 cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc
    23821 atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg
    23881 tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat
    23941 gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg
    24001 cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa
    24061 caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa
    24121 gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga
    24181 gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc
    24241 cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat
    24301 cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat
    24361 atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt
    24421 caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc
    24481 acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg
    24541 catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg
    24601 agatgtcatg ggtaaatacc atcctcatgg agactatgcg atctatgagt caatggtacg
    24661 gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc
    24721 tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc
    24781 tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga
    24841 tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac
    24901 aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt
    24961 agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt
    25021 attacctgga ccagattttc caacaggtgg tttggttatg ggaaaatcag gaatccgaag
    25081 agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat
    25141 gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa
    25201 attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt
    25261 gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag
    25321 cgcatctgtt atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt
    25381 caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaattcttga
    25441 aaactatatc gaacatcagc gcgaagtcat cacaagacgg acagtctttg aaaaagaaaa
    25501 agcagaagca agggcacaca ttttagaagg tttgcgtatt gctttggatc atatcgatga
    25561 aatcattgcg attatccgcg ggtctaaatc agatgacgaa gcaaaagcga cgatgatcga
    25621 acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt
    25681 gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga
    25741 agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt
    25801 agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt
    25861 cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa
    25921 caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg
    25981 tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc
    26041 tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata
    26101 cgaaattcca gaatacgggc gcacagccaa aggaatacca attatcaatt tactaggtat
    26161 cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca
    26221 ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc
    26281 taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa
    26341 tgtgctactg acagatggtc aatctaatat cattattgga acacataacg gctactctgt
    26401 cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg ttagaggaat
    26461 acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaag atcaagaagt
    26521 attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa
    26581 aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc
    26641 tggattgacc actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat
    26701 tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt
    26761 gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc
    26821 agaacaaaca gttgaagaac aaggatcaga agaatagcca tcaagactaa ttgtgtaagc
    26881 tagcaagaat gaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa
    26941 tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta
    27001 taatcagagc ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta
    27061 agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt
    27121 acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg
    27181 caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt
    27241 tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc
    27301 catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca
    27361 ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa
    27421 cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa
    27481 gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg
    27541 aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt
    27601 tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt
    27661 actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca
    27721 gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa
    27781 caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac
    27841 tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac
    27901 ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat
    27961 ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt
    28021 agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa
    28081 aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa
    28141 aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt
    28201 cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc
    28261 aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt
    28321 atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt
    28381 tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa
    28441 ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc
    28501 gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg
    28561 cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga
    28621 ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca
    28681 aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa
    28741 aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa
    28801 agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga
    28861 acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat
    28921 tggatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga
    28981 tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac
    29041 agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag
    29101 cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa
    29161 gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg
    29221 gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg
    29281 ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt
    29341 tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata
    29401 tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag
    29461 cttacatcgt tatcaacgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa
    29521 tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga
    29581 aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc
    29641 agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag
    29701 agtttccaag taaacctctg ctgacttata tcgaatcttc agcttcttca gcttcagagc
    29761 tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg
    29821 ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg
    29881 cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac
    29941 aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa
    30001 gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata
    30061 gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt
    30121 tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga
    30181 tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta
    30241 ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag
    30301 aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga
    30361 tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa
    30421 ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc
    30481 aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt
    30541 aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga
    30601 cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata
    30661 caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta
    30721 cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt
    30781 acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa
    30841 gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg
    30901 atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt
    30961 ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca
    31021 ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca
    31081 ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct
    31141 tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg
    31201 tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga
    31261 cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt
    31321 aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc
    31381 gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt
    31441 tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag
    31501 tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact
    31561 gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct
    31621 accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat
    31681 cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa
    31741 tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc
    31801 tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg
    31861 tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc
    31921 aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg
    31981 gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat
    32041 tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa
    32101 aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt
    32161 agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga
    32221 acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg
    32281 agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa
    32341 gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt
    32401 ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc
    32461 gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta
    32521 aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac
    32581 ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat
    32641 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt
    32701 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag
    32761 cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc
    32821 ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga
    32881 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    32941 tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt
    33001 tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac
    33061 ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat
    33121 aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata caatggttca
    33181 ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa
    33241 tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt
    33301 gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat
    33361 aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac
    33421 ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt
    33481 gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc
    33541 aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa
    33601 ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta
    33661 ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa
    33721 tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga agaatattat
    33781 gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct
    33841 ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac
    33901 cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga
    33961 agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca
    34021 cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc
    34081 gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat
    34141 tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg
    34201 atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc
    34261 gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat
    34321 gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca
    34381 gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca
    34441 ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc
    34501 taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat
    34561 ctatacgata ataataactt gaacactgtt tattcagaat aagggactgt gacaaaaacc
    34621 gtgtcacagt ccctttttcc gaacaaacgg tgttcaaaag cttgcatcta cggcactaag
    34681 cccaatcaga tgaaaaatat ggagtgctat ttttatctga ttgttcttac tgcttgatgc
    34741 agagcagctt tttcacaacc tctccactaa acggtgttct cctgccaact cctcggtcac
    34801 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgttcct tgtgactcgg
    34861 agctaaacgg caggctcaca acctcttggt aaacggtgtt caatcagctg accctcggta
    34921 ttagctcaaa aaacggcaaa atatgaggaa ctattttttc cgtttttttc tctaatactc
    34981 aggtcaaaac gctgatttca caacctctga ttcacggtgt tccagaagta gcttctacgg
    35041 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    35101 ttgaagctga ccacttctgt cacaacctct tattttgatt agaacttgcg aaaataaacc
    35161 ggttaaagtg tgtctagcgc aagagatttg ctataataga cgataagggt aaaatgtaac
    35221 gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga tctgccatac
    35281 catgtattag caaaagaaaa agttgatttt ttatcaatga atctccaatt gaatgatcaa
    35341 gagttagtag atgacctcgg aaagacattc aattatgacc gctacatgaa tcagctgaaa
    35401 aatggtgcga tgcctacgac atcacaaatc aatgttggcc gatatttaga atttttccgt
    35461 tcttatgcgg aaaaacagat tcctgtttta tatgtggctt tttccagtgg attaagcggc
    35521 tcttacagta gtgcgcttca agcggttgaa ttgctgaagg aagaatatga agaccctgag
    35581 atctatgttt ttgatacaaa agctgcaagt attggggaag ggcttttagt attggaagct
    35641 gtaagattga aaaaagaagg taaaagcatc gaagaaaccc tagagtggct ggaaagtaat
    35701 tatttgaaag tccattcatg ggtaacagta gacgatctga aatatctaga acgaggcggt
    35761 aggatttcca aagcaagtgc tactcttggc agtctattga gtgtaaagcc tattattacg
    35821 gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa caagtcgatc
    35881 cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca aatcgtttat
    35941 gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt ggaagagcag
    36001 ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag tcacacaggc
    36061 tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta actcattctg
    36121 taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat agatgagcat
    36181 cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca ttatgcccat
    36241 aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata tgtcgtatca
    36301 gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa tgtttggata
    36361 aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa gccgtcattc
    36421 aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac aagtacgtta
    36481 gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc agataaaaca
    36541 ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa gcttcttaag
    36601 aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa aatcgttaga
    36661 tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt tgtcacaccc
    36721 caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa tagcgttgtt
    36781 gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa attaaaagat
    36841 atatcatcta aaattttctt tccatcaatc gttacactta cattttctac ctgcaataag
    36901 tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct agaagaaggt
    36961 tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc ttgttttgat
    37021 tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat ttgttcttct
    37081 tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc caaccaaaaa
    37141 tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat atgtgtacat
    37201 actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc aaaattgatc
    37261 aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg ttcgtctagc
    37321 aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac cttttgtcca
    37381 ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag accttgcagt
    37441 aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc tccttctaat
    37501 tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat cgcatctttt
    37561 tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag tacagtatag
    37621 tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg tcccatagta
    37681 acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt tgatttacct
    37741 gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat attcacatca
    37801 tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat caattttttt
    37861 cctcactttt atcattatta gttttgacaa gtcttataga attatatagg ggaaaggtaa
    37921 aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga aaaaaataaa
    37981 aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca gcataatttt
    38041 tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag aaggattgaa
    38101 aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt tatttgtact
    38161 tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg tagtagtctt
    38221 tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca gattaaaaga
    38281 gctaacagga tataaaggaa agatgccctt aataatcggg atcatcgata ttctagttgg
    38341 cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg tttttgcagt
    38401 atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa gaggagtaag
    38461 tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat taggaatttt
    38521 cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg gattctattt
    38581 tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat caaacaaata
    38641 aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct tttttatcat
    38701 ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa aaatgtgaga
    38761 agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc acaacctctt
    38821 ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt tgaatgcttg
    38881 gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg gcaatgggcg
    38941 cttgttattg atcttcacat cgattactac aggacgatct gattttgcag ctgcatcaaa
    39001 ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga gcgcttctcc
    39061 aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt ccgtatcttc
    39121 ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa taattggaag
    39181 ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc catcaccact
    39241 taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc ctggcacacc
    39301 attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt tcatattcaa
    39361 taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg catcgtcttc
    39421 agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt cctctcgatc
    39481 ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg ctaggtacca
    39541 gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg tgtttgcatc
    39601 accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat cgatatcgat
    39661 ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt cgctaccgac
    39721 aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag cgacacgtcc
    39781 agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta gaacggaagc
    39841 gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc cgtttcgcgt
    39901 tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca aagcagctag
    39961 caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg tggagaaagg
    40021 ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag ccacgccttt
    40081 atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt gtggtgtcat
    40141 gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta gttcctggaa
    40201 agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta gaggcacatg
    40261 gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc ctgcagagcc
    40321 gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga gtgcgccgac
    40381 ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata gcgcatccat
    40441 aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc agctttcaag
    40501 aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact cctttagcaa
    40561 tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa atcccaacta
    40621 attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt agtacataga
    40681 tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa ccttggcagc
    40741 aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc gggttggttt
    40801 ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt gcttctggtg
    40861 tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa gcagaagaaa
    40921 atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt gtgtttttat
    40981 atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa gcagaaggat
    41041 tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg cataacattc
    41101 ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat aatccgacag
    41161 ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac ttcccagaag
    41221 gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag gcctttgttt
    41281 acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg ctactagtaa
    41341 cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca atgatttatg
    41401 tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa cgtcattttg
    41461 cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg gcattaggat
    41521 aaataaaaaa aatatatatg atatatcaaa tagggccgcg aacttatatc gcgaccctat
    41581 ttttttagta agttatatca taatgaagtg aatggtatca tacattaata atagtttttt
    41641 ctatgtgtat ttcctttgat ccagcgaaca agccagatga taagtgcgat tgggattaga
    41701 actttgaccg caaaccataa aagcccgctt aggacactga caaaaagact gatgacgaag
    41761 ctagctgcaa ttagcactaa aagaaccatg aagatattta agaacatatt tttttgacgc
    41821 atacaaaacc ctcctcttgt tcattcttta ctatagaaaa agtataacag ggattgttta
    41881 aaaacacctc caactgaaga ctgattttta cttcatctac ttaaatgatc aaacaattgc
    41941 tttttctctg gtttatcatc taaaatagag cggaaaagga ggagaatatc atgcctaaaa
    42001 atcgtcttga agcatttaca gatgctgtaa tcgcgattat catgactatt ttagttttag
    42061 aacttcatga acctaacgga gacacgtggg aggcgctcgc tgcattaggt taccgatttt
    42121 ttatctatat tgtcagtttt atcattttag cgatttattg gaacaatcat catcatttat
    42181 ttcaagttgt acataagata aatggacggg tcttatgggc gaacagtttc tttgtttttg
    42241 cgctttcgct ttttccgttt gcaacttcat ggatcagtga gtacatcaat agcctggctc
    42301 cagaaatgac ttatggtttt gttaccttga ccgctaatat tgcgtattat atcttggcaa
    42361 aagaacttgt caaagcaaat aaaggccacg agaaagcatt gaaggcgatg ttgaataatt
    42421 acaagaaatc gtatctatcg attggtttga atgtgttagg aattgtctta gggtatttag
    42481 tagcgccagt agccgtgctg atcgcaaaca tcgtgattct agctgcttgg atcatcccaa
    42541 gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt aagagaaaaa
    42601 tatagaaaaa ataaataaac gatcaaatat ccgagtgata ctagtattca aagagggatg
    42661 cagatggatc aaaaaagaat tcatgtagga ctgattacta gttttgtcac gatgcttatt
    42721 tatttggatc aaggtcattt gacttggaca gtagttggtg tcgtaggatt tttgacatta
    42781 ttcacagcaa taaaaaaata aacatcttta tgtaatctga tgttctatct atttaagaaa
    42841 agcatgttta cagcagtatg attctgttgt aaacgtgttt ttatttgtgg aaaatttaat
    42901 ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc cacagtggat aacttcgtaa
    42961 gagataaaaa taaaaaatta gtcaaagaag ggcgtataga tttcttttga taggagatat
    43021 acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt gtggtttttg
    43081 gaataaaaaa ataacatatc tcattttacg cttttgttat tttatactta aaaaataacg
    43141 acacagaaaa taccgtgtct cagatttctc tactacttct aaaaactact gtttttttcc
    43201 tttgaaatgc cttcaaaaag taggatcagg cagaatatac tggcagaatc aaggagaagg
    43261 gagcctaaat aaggagaaaa accatagaaa atataaagta attacttata aaacaagctt
    43321 tttatagaaa aataatatac ctgagtaggg tataaattaa gacggaattc ctttctagat
    43381 tgagatattg actaggaaga atgttaagat tttaagcctt taagctaaaa aaaaaccctt
    43441 tttgttaaat aaggggaata tagcctgtca gtttcaaact gcaagtcgtc ctgctgcaat
    43501 aatatagtag attagagatg aaaatagatt tttttaaacc aatgactgaa taaaaagacc
    43561 gtcatagata cacattggaa tgtgtctatt acggtctttt tttacgaacg attttattga
    43621 agacggacag ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa taaaatgtat
    43681 cccaaaataa tggctagacg gtctaactta tttgtcgata gatgtttgtt tagaagttga
    43741 taggcccata aacctatcag tcctcctgtt gtattagtta tcaaatcagt gatgtccgct
    43801 acgccaatac cgaataggaa ttgaatcaat tctgccaaga agcttacaag gaaaataatc
    43861 cctaattttt tagaaaaaga aagtctcttg aaattgacat tcaaaagcac cccaaaagga
    43921 aaaaagacaa tcaagttata agtggtctca cgtaataccg tcgtattttc aaaagaaaaa
    43981 gtagaaaaag ggacgagatt gacattgctg taagaataag tgagaaattg tggaagatta
    44041 aatgatagct tgaataaaat caaccataat agaaagaata aataaatccc taataaaaat
    44101 tttgatacat accgcataga catctcctcc atttaaaaag tataccatcg aaaaccgctg
    44161 aaaaatcata aaaaagtatg aagtttaagg accaacgtag ttatcgtacg aaaattagaa
    44221 gctttttttt gttgggaatc cggtttcttt aacaaagcta agatagcgag ttgcttggaa
    44281 aagttgctat cttttttctg tcaaaacgac tcggaatgtt cgttttttgt tctaatttct
    44341 ccaagttgtt tactttttga tactaagttt atgaaaaatc aagaaatagt tgttatacta
    44401 ggtaatgctg acaaacggga ttggcatttc atcttggttc agcttttttt ccaattacta
    44461 ggaaggaggg atagattgtt caagacttat tttcgtttca ataaaataca agataatcct
    44521 tttcgtatca tgaatctgat gctacttatc tttattgtat tatggttagg ttacataaat
    44581 catgattttt tgatttcaag tttttcatca ttagggatct ttactttttt ctattatcaa
    44641 aacataccga tgaaatattt gctgaaacgt atgtttctga tcggttgcgg actgttggtt
    44701 gctttcgttt tggggatctt atcgacttat gttttatggc ttgaaccatt tgccgtagct
    44761 ttggttgctt tttccagcag attcgtgttg cgattattcc atatttctaa accaggtggc
    44821 ttgttttttg cgatgctttc tgccatggga acgagcatgc agctgccaat cgctcaatta
    44881 ccgatcgtct cactttattt ttttatgggt gtcgtttttg ccctgattgc ggcagtgatt
    44941 acaaaattac tggattcacg gcctgagcag acaattgaaa aagcaacttt aaaagaaaga
    45001 tttcacgaag agcctttagt gatcattgat tctgttttct acagcgctgc cttatttctt
    45061 tccgtttatg tcagtcacgg cttgaatctg cataatcctt attggttgac cttgtcatgt
    45121 gcatcgattt tgctagcaga aaacttggat gcgatgaagc atcgacaagt ccaatatctg
    45181 attggatcga tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc gtttacgcag
    45241 ttgcagacca tttttctgat cacttttcta tatggaatcg cacaatttct agttgctaga
    45301 aattatgcag ttgctaatat ttttcttaat ccgatggcgt tgatgctatc tacacttatt
    45361 cgtggagcat acttgatttc tttgatcgaa tatcgttttt tagggatcgt cataggaagt
    45421 ttcatcggtt taggtgttgc gtgggtaatg acggttggtt tgcaacatta tttaacggtt
    45481 gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc aaaagaggtt atgacaagat
    45541 ttttgtcaca acctctaatt caaggtgttc aaaagccaga ttctcagcat taagtcaaat
    45601 tcacacaaaa atgtggaaag gaactttcaa aaaaatttta tatttattct atcaaaaaat
    45661 tttgtttatg atggtaactg aataaacttt cctgagatgg tcaatgcttt ccttgaaagt
    45721 cttacagata ttctttcttc atagaaagtt taagttttat agttagtgta caataaaagt
    45781 atgtttatac catatggtgt ccggataatc agtcatttaa acaatgaggt tcaaagatat
    45841 gaaaagggga aatttgatga agaaaataag caatgaaatc caaattctgg cagaaacttt
    45901 aactcttccc aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca tccttgatga
    45961 ggtgtttgag caatatagtc atgaactaat ggatcctaat aaagcaaagg cgactgcaaa
    46021 gaaaatagac aaagtctatg aagaggaacc ttttgcagca ttagctattt atctatatgc
    46081 agcttctcaa agttggaaaa aaatttatca accattgaag attccaagaa ctatctattt
    46141 ggcaacaatg aatgctttta ctagatttat tcatgaacat ttccaagtaa caggaagcta
    46201 tcgctttgac cgtggttttt ggatttggca ttacactagt ggcttgatct ttcggattgg
    46261 tgaattggag tacgaaatga catattttcc gaaagggtac acggatctaa gattagaagg
    46321 aaagcagtgc ctttctcttc atattccttc cgatgcagat ttatctcaga ataagataag
    46381 agatagttac gaaagtgcca ttcgtttttt tgaactttat tttcctgatt accattacca
    46441 agtgatgtac acagatactt ggttactatc acccaatctt actaaatggt tgaaaaaaga
    46501 gtcaaaaatc tgtctttttg cagccgatta tcgattatta tcagttgatg aacaggacga
    46561 tagtggagtc ccttggatct ttggacgagt agatgcccaa atacatgatt atccagaaag
    46621 tacgagttta cagcgacaag caaaagagca attgcttgca ggcgagcaca ttggctctgg
    46681 cttaggtatt cttacttcat aagtttagta cttcgaaata gtttaaaaga gcgtgagaca
    46741 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca
    46801 actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct
    46861 tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt ctatcgaaca
    46921 cacagtacgt tcaataggta aactagctat aacaatagat attaaagtta acggacaaga
    46981 gggccttttt taggtagcta aaacgtaata atgaaaattt atttccagag ataggagtca
    47041 atcagctagt ctgatcgact gttttaacaa gttgactttc attttaactt ttgataccat
    47101 ttaaaacaca aatcgtaatt gtttcgattt gtgtttttta gcatgactac aatcaaagaa
    47161 gaaagtgggt cgtccatatg gaaaaaacag atatctcaag tgcataccgc cgcttgaaaa
    47221 gcccaaatat caaaacacga aaacgtgcgc tgaagatcat caaagatgtg aaacggaata
    47281 gtgggaaaag gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa cgtttttcgg
    47341 ctattttcaa taatcggttt ttgtgtaagg tttccgaaag aactgctgtt gattttccat
    47401 ttttaacata tgataaattg gtagaatctt tcttttcaaa ttgataccat tccctaattc
    47461 cagttatata gttaactaca gaaccacaaa gcacgtacgt ttataaaagt attatctgta
    47521 gttatttttc aattcccttt caacctctta ttttgaccag tagtattgat cactttacct
    47581 tttaacacct ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg taactacata
    47641 atcaaaggtt ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc aatacggttg
    47701 caaataacca ttatcgttta ataagcagtt acgaaagctc ccaatatctc taacgttcag
    47761 ctcactccat ttcttttctt caaaaaaatc agtgattcgt atctctttct atttgtcgaa
    47821 aaagttaact ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa agcagttact
    47881 attgctagaa taacatcaag gaataataag ttttggtttt cagataatag tcgataagtt
    47941 gtgtattgga attttagata cagtaataaa gtatttttca tgaaattttg tgagaatcac
    48001 tattacaagt atctttaata aaattaatgg ttaaggagga acttaaagta gaatgtaaat
    48061 gaatagttgt agtcagtcga ttgtgatact agactcaaag ttgcaggaat aagtttttca
    48121 atttgtgatt ggaagataaa aaatgaaaaa gaagggcttt tttttatact ttgttgattg
    48181 tagtgtttat tttccctctg tttagccaag taacattttc tatgactcaa ccagtttcaa
    48241 gtgaggaaca tgataacctt tttcgtgcta cagaagagtt taagaaatat caggaaaata
    48301 ttgttccgga gactccatta gctagtgagc caattactga tattgatgga aaccaaatag
    48361 gatggtatat acagtacgaa gttatggtgg taaataatga ctcaatagta aaaaatacag
    48421 taggcttaga gctaacaccg attgaaaagg tgagttcatt gttaacgtat atttatactg
    48481 atgaaagtga cgagactgag atcattttaa cggattactc aaaaattgct actgataaaa
    48541 agtatatatt gtggatgtag acactagtga aatactttat gatatagaaa ttgatgaaaa
    48601 tttactagat gtagcacaag aaatcgatca tcaaaagcag gaagctattc gatatgctgg
    48661 tagaaaaggg aaaagaaaat atttattacg tgaatgtagc aaaagttaga gaagatgaaa
    48721 atgagtggaa agaattcaag agtcgctact caattaactc gactccgaca tttacagttt
    48781 acagagaagg atcgatagaa aaaactgttt tttggactaa agaaagtgga atgtctttag
    48841 ctgaagtaga agagtttttg gattatgtta gtatgcaaca gtaagggagt tcttattatg
    48901 aaaaattttg acacatatta tgttattgtt ttttcgatat ctttgctgtt aatattacta
    48961 actttaatgc agtataaaga gtatccagag tattttattt taacttttgt atatttagta
    49021 agtagctaca cacttctata taaattcaga agttcttcta gagtttctaa agtttgtttc
    49081 ttaattagct tattgattct ataatatttt tgatttatat aggaaattaa aaatattata
    49141 gaatcaacac tcagagaacg ggttggtttt taatagattt aataagtact gataatgaaa
    49201 tttctcctgg ttgggaaaac gaagttggtt accctgtaga agaatttttt caagattact
    49261 ttgcggagta tgccgatagg ataatgagag attacgtaac agataaaact caagaagtca
    49321 tcaatcaatc ccagtcatta aatgaattat ctgataaggc gaaagaagaa gcagttgcca
    49381 ttatttcttc agggattagt gactatattg atatgagtgg tgaaatcaat tatggcgata
    49441 aagacaaaat tgtacgcatg ataaaagctt ttatcaaaac ttttgatgtg aaggcagatc
    49501 ttaagtttaa tgatacttac ctagtaggaa aattaattaa tatccttggg acagagaaag
    49561 atacggaaac gtttatttcc tctttgtcta tgttgtttac agataagcat ttttcaactc
    49621 agcttggtgg agaggaacta gtggaaatct tttaaccatt acgcccagca ttaataaaag
    49681 tatatcaaaa aagtactcct gatgattttt aagattggtt tgagaaataa tgtgaaaaca
    49741 ctatttttaa tgttaattat agatagagat gaacgcgtca tatggttaaa agaatgaaaa
    49801 agtcaaagat acac
//
LOCUS       DASHFF010000016        47699 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.016, whole genome shotgun sequence.
ACCESSION   DASHFF010000016 DASHFF010000000
VERSION     DASHFF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.016"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            101..544
                     /locus_tag="U2I75_001020"
     CDS             101..544
                     /locus_tag="U2I75_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1696170.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="U2I75_001021"
     CDS             complement(550..1734)
                     /locus_tag="U2I75_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN1696171.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="U2I75_001022"
     CDS             2002..2700
                     /locus_tag="U2I75_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1696172.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="U2I75_001023"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="U2I75_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEN1696173.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="U2I75_001024"
     CDS             complement(3510..4043)
                     /locus_tag="U2I75_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696174.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="U2I75_001025"
     CDS             complement(4340..6721)
                     /locus_tag="U2I75_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1696175.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="U2I75_001026"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="U2I75_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN1696176.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="U2I75_001027"
     CDS             7422..7961
                     /locus_tag="U2I75_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN1696177.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="U2I75_001028"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="U2I75_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN1696178.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="U2I75_001029"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U2I75_001029"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="U2I75_001030"
     CDS             9068..10234
                     /locus_tag="U2I75_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN1696179.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="U2I75_001031"
     CDS             10280..11776
                     /locus_tag="U2I75_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN1696180.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="U2I75_001032"
     CDS             11857..12540
                     /locus_tag="U2I75_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1696181.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13185..14375
                     /locus_tag="U2I75_001033"
     CDS             13185..14375
                     /locus_tag="U2I75_001033"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN1696182.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14477..15430
                     /locus_tag="U2I75_001034"
     CDS             14477..15430
                     /locus_tag="U2I75_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN1696183.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15499..16848
                     /locus_tag="U2I75_001035"
     CDS             15499..16848
                     /locus_tag="U2I75_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1696184.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17031..17354)
                     /locus_tag="U2I75_001036"
     CDS             complement(17031..17354)
                     /locus_tag="U2I75_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696185.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17614..18645
                     /locus_tag="U2I75_001037"
     CDS             17614..18645
                     /locus_tag="U2I75_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1696186.1"
                     /translation="MYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHF
                     RDNTVLVLSEPSTYLPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCL
                     SLFFELTQLSGLYFIYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDEL
                     SYEKGKTVSLTRRLVAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVY
                     FMIVPFITNGWTLGKKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQ
                     IMNSTTNTVLQMSAVFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIG
                     QTKDDDSRN"
     gene            18715..20064
                     /locus_tag="U2I75_001038"
     CDS             18715..20064
                     /locus_tag="U2I75_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1696187.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20109..20540)
                     /locus_tag="U2I75_001039"
     CDS             complement(20109..20540)
                     /locus_tag="U2I75_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1696188.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20928..23570
                     /gene="alaS"
                     /locus_tag="U2I75_001040"
     CDS             20928..23570
                     /gene="alaS"
                     /locus_tag="U2I75_001040"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN1696189.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23770..24366
                     /locus_tag="U2I75_001041"
     CDS             23770..24366
                     /locus_tag="U2I75_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1696190.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIVTIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24488..25636
                     /locus_tag="U2I75_001042"
     CDS             24488..25636
                     /locus_tag="U2I75_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1696191.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25674..26087)
                     /locus_tag="U2I75_001043"
     CDS             complement(25674..26087)
                     /locus_tag="U2I75_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN1696192.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26166..26597
                     /locus_tag="U2I75_001044"
     CDS             26166..26597
                     /locus_tag="U2I75_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN1696193.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26696..26929)
                     /locus_tag="U2I75_001045"
     CDS             complement(26696..26929)
                     /locus_tag="U2I75_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN1696194.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27112..28782
                     /locus_tag="U2I75_001046"
     CDS             27112..28782
                     /locus_tag="U2I75_001046"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN1696195.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28929..29315
                     /locus_tag="U2I75_001047"
     CDS             28929..29315
                     /locus_tag="U2I75_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696196.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29302..30000
                     /locus_tag="U2I75_001048"
     CDS             29302..30000
                     /locus_tag="U2I75_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1696197.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30005..30469
                     /locus_tag="U2I75_001049"
     CDS             30005..30469
                     /locus_tag="U2I75_001049"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN1696198.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30466..31377
                     /locus_tag="U2I75_001050"
     CDS             30466..31377
                     /locus_tag="U2I75_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1696199.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31612..32151
                     /gene="pyrR"
                     /locus_tag="U2I75_001051"
     CDS             31612..32151
                     /gene="pyrR"
                     /locus_tag="U2I75_001051"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN1696200.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32165..33448
                     /locus_tag="U2I75_001052"
     CDS             32165..33448
                     /locus_tag="U2I75_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN1696201.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33461..34387
                     /locus_tag="U2I75_001053"
     CDS             33461..34387
                     /locus_tag="U2I75_001053"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN1696202.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34406..35686
                     /locus_tag="U2I75_001054"
     CDS             34406..35686
                     /locus_tag="U2I75_001054"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN1696203.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35691..36767
                     /gene="carA"
                     /locus_tag="U2I75_001055"
     CDS             35691..36767
                     /gene="carA"
                     /locus_tag="U2I75_001055"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN1696204.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36772..39957
                     /gene="carB"
                     /locus_tag="U2I75_001056"
     CDS             36772..39957
                     /gene="carB"
                     /locus_tag="U2I75_001056"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN1696205.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40011..40787
                     /locus_tag="U2I75_001057"
     CDS             40011..40787
                     /locus_tag="U2I75_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN1696206.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40784..41716
                     /locus_tag="U2I75_001058"
     CDS             40784..41716
                     /locus_tag="U2I75_001058"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN1696207.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41713..42429
                     /gene="pyrF"
                     /locus_tag="U2I75_001059"
     CDS             41713..42429
                     /gene="pyrF"
                     /locus_tag="U2I75_001059"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEN1696208.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42436..43065
                     /locus_tag="U2I75_001060"
     CDS             42436..43065
                     /locus_tag="U2I75_001060"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEN1696209.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43053..43367
                     /locus_tag="U2I75_001061"
     CDS             43053..43367
                     /locus_tag="U2I75_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696210.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43405..44046
                     /locus_tag="U2I75_001062"
     CDS             43405..44046
                     /locus_tag="U2I75_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1696211.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44089..44781
                     /locus_tag="U2I75_001063"
     CDS             44089..44781
                     /locus_tag="U2I75_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN1696212.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            44778..45680
                     /locus_tag="U2I75_001064"
     CDS             44778..45680
                     /locus_tag="U2I75_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1696213.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(45838..47544)
                     /gene="efbA"
                     /locus_tag="U2I75_001065"
     CDS             complement(45838..47544)
                     /gene="efbA"
                     /locus_tag="U2I75_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN1696214.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        15522 a         8347 c        10270 g        13560 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 tttattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt tgtcacagtc
    12781 cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa aattaaaaat
    12841 tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa taaataaatt
    12901 aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt gtgttgtatt
    12961 tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc atactatata
    13021 agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa ttctcataat
    13081 cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga cacgatatat
    13141 gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct tccaaagaac
    13201 taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta tacaatacga
    13261 tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa aagatgatca
    13321 atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa aaagcatgta
    13381 ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc aatggttcgc
    13441 tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca gaagcagcaa
    13501 tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta atgacaaaag
    13561 aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt tgccgcttat
    13621 ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag aaaaaagcaa
    13681 aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat ggtgttttct
    13741 ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa aaatatggac
    13801 tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt gcaggaaccg
    13861 tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta tcgaaagcta
    13921 taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg ttgaaagcta
    13981 gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca gctgcattgg
    14041 cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg gaaaatgcta
    14101 attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct gaaacaccta
    14161 ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag aagctgatcg
    14221 aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg acaggcagga
    14281 tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca gtagcagttt
    14341 atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt tcgtctattt
    14401 aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt agtaaaaaag
    14461 atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac aaattggttc
    14521 agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg cgacagatat
    14581 acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg taatggatta
    14641 cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc atttggctgc
    14701 tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga acgtgactgg
    14761 gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta cgccaagttc
    14821 aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata ctcttcagcg
    14881 tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg attattatta
    14941 tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt cttatgcaac
    15001 cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag caatcaaaga
    15061 aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt atatgccaga
    15121 tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg ttcatcgaaa
    15181 tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat ctattcaaaa
    15241 aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt caatagcaga
    15301 atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct tttcgccgaa
    15361 atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa aattggtaga
    15421 gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga ttggctatgg
    15481 tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga agtaaaaata
    15541 gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt tatgggcaca
    15601 gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac aggatccgcc
    15661 agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc aggactaaca
    15721 ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata tatcgaacgg
    15781 atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat ttattttcca
    15841 gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct atttggtctt
    15901 tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct gttgatcaat
    15961 tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg caagcttatt
    16021 cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga cttccaactg
    16081 tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc gggcttgcta
    16141 gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg agaattgaaa
    16201 aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat catgatcgtt
    16261 tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt cgcaggaaac
    16321 agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg aagactggtc
    16381 acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct tactggaatg
    16441 cgtctccctt atgttatggc aaaagaaaac aacctgcctt ttagcaagct ctttgctaaa
    16501 cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt catcgctatt
    16561 ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt tgtcatttgg
    16621 attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga acctgatttg
    16681 tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat cattggaggg
    16741 acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat ggggatagta
    16801 ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct aaaacattcg
    16861 aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat gaggagtacg
    16921 tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt ttgtctacga
    16981 tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt ttactttgat
    17041 ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac caagcaacca
    17101 gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt agacaaagca
    17161 tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag attgaagact
    17221 agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt agttcttttt
    17281 cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc aaaaacaaag
    17341 tacatattca gcatctcgac accccctttg tgtttatctt actttacttt gaaagaaatg
    17401 agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat aagagagata
    17461 caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt ttgcgctgat
    17521 tgtttttcca tttttagcct tagcgatctc atctgttttt ttatttatga atatcgaaag
    17581 tatggaacat ttctagtaac aagggcagtt attttgtatt ctttcgtttt ttatttattg
    17641 tgtgcgtatt ttttggtgat cttaccactt ccggcaaagg agacggttgc ccaaatgaca
    17701 gggccaaaga tggagctgca tttatttgct tcatggcaac atttcagaga caacacggta
    17761 ttggtcttgt ctgaacctag tacctacctg ccggcaatga agcagagtgt atttttggaa
    17821 ccagctttta atgttttatt actcatacca tttggcattt atttaagata ttatttccgg
    17881 ctttctttta tgaaaacgat gattgctagt ttctgccttt ctctattttt tgaattgacg
    17941 cagctaagtg gactttattt tatctaccca agaccatacc gcttatttga tgtgaatgat
    18001 ttagtgacaa atacattggg tggaatcatt ggttttgtcg ttacgccgtt atttacattt
    18061 atgcttccta caagaagacg aatggatgaa ctttcttatg aaaaaggaaa gactgtctca
    18121 ttgactcgac gcttggtcgc ttatttgatc gactggacat ttttatctct tattaatttg
    18181 gtagcggcga ttttctttaa aattgcgttc gattcagggg cagttacttc tcatatctgg
    18241 tggcttttat tagaagtagg ggtatacttt atgattgtac cgtttataac caatggatgg
    18301 acacttggca aaaaaattgt gcgtatccgt ttggtggaag aagaccgcga acgaatttct
    18361 ttaaaagcat tgatgatccg ctacgggtat ctgtactttc ttttttatgg tttaagctgg
    18421 ttggggcagt tgcttcagca aatcatgaat tcgacaacga atacggtgtt gcagatgagt
    18481 gcagtattca tttctctcgg tgtcttgtta ctacaaggta tttttatttt aaatatcttg
    18541 ctatcatttt tgagaaaaaa acggattctg ttttatgaaa aagctagtca cacatatact
    18601 gttagcacga ttggccaaac aaaggatgat gattctcgaa attagctgag caaaaacgct
    18661 ttgtctgtgt tataatgcca aagtcgtgat aatttaaaga gaagaggtgc acgaatgaat
    18721 aattttgata tattccgctt caaaccgttt atcaatgaag cgttaaccgc aaagggcttc
    18781 aaacagccca cagaggtcca ggaacgtttg attcctttaa ttgcaaaagg aaaaaatatc
    18841 attgggcaat ctcaaactgg tacaggaaaa acacatacgt ttttgttgcc tttgtttgaa
    18901 aaaatcaatg cagaaaaaga ggaagtacaa gttgttatta cagctccaag tagggaacta
    18961 gctgcacaga tctatgaagc agctacacaa atcgctaaat tttctacacc ggagatccgt
    19021 gtctcgaatt ttgtcggcgg tacagacaaa cagcgtcaat taaatcgtct gcatcaccaa
    19081 cagccacagg ttgtagtagg cacgcctggt agaatattag acatgatgaa tgaacaagcg
    19141 ctgaagattc acaccgcatt tgcatttgtt gtcgatgaag cggatatgac attggatatg
    19201 ggttttttaa atgaagtaga ccaaattgca agtcgattac cagaaaagct gcagttttta
    19261 gttttttctg ccactattcc agaaaaactt cgtccattct tacgaaaata catggaaaat
    19321 ccaatcattg aagaaatcaa accaaaagca gttatttctg aagacattga aaattggctg
    19381 atttcaacaa aaggtaaaga tgagaatcaa atgatttatc aactgctgac aattggtcat
    19441 ccttatttag caatcgtatt tgccaataca aaacagcatg tggatgaaat tgccgattac
    19501 ttgaaaaacc aaggcttgaa agtagcaaag attcatggag acatcacccc aagagagaga
    19561 aaacgagtga tgcgacaagt acagaatctg gagtatcaat atgttgtagc gactgattta
    19621 gctgctagag ggatcgatat cgaaggtgtc tctcatgtta ttaatgcaga aattccagaa
    19681 gatctagact tctttatcca tcgtgtcgga cgtactggac gtaatggctt aaaaggaacg
    19741 gccattacgt tatattcgcc aaatgatgag caagccattg atgcgataga aaaattgggc
    19801 gtctcatttg aaccaaaaga aatcaaaaac ggggaaatcg tcaaaacata cgatcgtaat
    19861 agacgtgcca aacgagaaaa atcaaaagat gtattagatc ccacgctgat tggtcttgtg
    19921 aagaagaaaa agaaaaagat caagcctggt tataagaaaa aaatcgattg ggcaattgca
    19981 gaaaaaaata aaaaagaacg caaaatcgaa cgcagacagc aaagccgtac tgctcgtaaa
    20041 aataagaaaa attcacataa ataaaaaaca agaggttgtg acaaaaggcg tgtcacaatc
    20101 tcttgttttt aagctagact ttttttcatt ccgatacaaa gtacttcatc ttcaataaaa
    20161 ggttctgtgc aagtatgata cccttgtttt tcataaaaat ctacagccgt catttcagct
    20221 actacataac tagtctgaca accttctgaa accgcccgtt tttcagcagc ttcaagcaac
    20281 tgaccgccta acccttttcc tcggtattct tttgctacgc aaaatcgttc cggcttgaag
    20341 caagtaggtg ttttcttttc ataacgcaaa gtagctactg gtcttttttg atcaagaaat
    20401 agataatagg tttgttgcgg gatatctttt ccgtcgaatt ctagttctgc aggaatccct
    20461 tgttctaata caaaaacttg atatctcaaa aagcagctag ctgcgcgtat ccattcatcc
    20521 gctccaacta tttttttcat catattcctc cttgtcatga atagcagaga tgtattgatt
    20581 cctttattct tatcgtacca aaaatcgtgg agtatgacca aaaggaaatg aaaataaaaa
    20641 ttgagatatt gacagtgatc aatagaatgc gtatactaat taagtacgaa aaggatatga
    20701 tttgtttttt aagactcaca gaaaattctt tcttggctga aaaaagaata gtcactaatc
    20761 aaattgctcc ctttttctat tttggtagaa ataccgacgc agctcagcgt tatcgagttt
    20821 aagaggtaat gattacacat cattgcaatc aaggtggtac cgcgagtttt cgtccttggc
    20881 tgtgatggtg tttttttgta tttaatttat agaaagcagg taatgacatg aaacaattaa
    20941 gtagtgcaca agttcgacaa atgtttcttg atttcttcca atctaaagga catacgattg
    21001 aacctagtgc gtctttgatc cccgtgaacg atccaacctt attgtggatc aactctggtg
    21061 ttgcaacatt gaaaaaatat tttgatggtt cagttgtgcc agaaaatcca agaatcacaa
    21121 atgcccaaaa atctattcga acaaatgata tcgaaaatgt cggtaaaaca gcacggcacc
    21181 atacgatgtt tgagatgctg ggtaattttt cgattggtga ctatttcaaa aatgaagcca
    21241 tccactgggc atgggaattt ttgactgatc caaaatggat ggcattcgat ccagaaaaac
    21301 tttatgtaac agtttatcca aaagatacag aagcaaaacg catctggcat gaagaagttg
    21361 gcctgccact agatcacatt attgatgtag aagacaactt ctgggatatc ggtgcaggcc
    21421 catgtggtcc ggatacggaa attttctatg accgtggaga agcttataac gatttagcag
    21481 aagacgatcc tgaaaactat ccaggaggag aaaatgaacg ttatttagaa atctggaact
    21541 tagtattttc agaattcaac cataagcctg ataacacatt tgaaccactt cctcataaaa
    21601 acattgatac ggggatggga ttggaacgca tggtctcaat cgttcaagat gcgtcaacga
    21661 actttgagac agacttgttt atgcctatta tccacgccgt agaaaagcta agcaaacaag
    21721 taaaatatgg tgaaaaccca gtaacagata tttcttacaa agtaatcgca gatcatattc
    21781 gcgccctttc atttgctatt ggcgacggtg cattgccatc aaatgaaggg agagggtatg
    21841 tcttacgccg tttgcttcgt cgtgctgtca tgcatgggaa aaaattaggt attgaacaag
    21901 cattcttata tcgtttagtc cctgttgttg gggaaatcat ggtaagttat tatcctgaag
    21961 tacttcaaca aaaagaattt attgaaaaag ttatccgtac agaagaagaa cgtttcaatg
    22021 aaacaatcaa tgaaggtttg gaaatcttaa atcaagtgat ccaagaagta aaaaacgaaa
    22081 acgggacgac tctagcagga aaagacatct tcaaactata cgacacatat ggcttcccgg
    22141 tagaattaac ggaagaggtg gcagaagatg aaggactgaa agttgatcat gctggttttg
    22201 aaaaagaaat ggatgcacaa cgcgaacgtg ctcgctctgc ccgcagtact gaaaaatcaa
    22261 tgggggtaca atctgcttta ttgacagata tcaaagtaga aagtaaattc attggataca
    22321 cagaacaaga atcagatagt caattattgg tcatcgttaa aggtgagtct cttttgaatg
    22381 aagtcgcaga aggtgaagct caaatgatct ttgatcaaac acctttctat gcagaaatgg
    22441 gtgggcaagt agcggatcaa ggatggatca tgaatgaagt aggcgaaatc gtagcagttg
    22501 ttacagatgt tcaaaaagcg cctaatggtc aatttatgca caccgtagaa gtaaaagcac
    22561 caatgagtga aggacaaacc tatcatctat tgatcgatca atcgatgcgc aatcggatca
    22621 tcaaaaacca tacagcaacc catctactgc acaaggcatt gaaagaagta cttggcgagc
    22681 atgcaaatca agctggttct ttagtcgctc ctggacattt acgattcgac tttacccact
    22741 ttggtcaaat cacttcagat gaattagctg aaatggaaag aatcgtcaac gaaaaaatct
    22801 gggaagctat cccagttgaa acaattgaaa cagatatcga tacagccaaa aacatgggtg
    22861 caatggcttt gtttggtgaa aaatacggac atgacgttcg agtagtaaat attggcggct
    22921 ggtcaatcga actgtgcgga ggcacacatg tagcaaatac agaaaacatc ggaatcttca
    22981 aaatcgtttc tgaatcagga attggtgcag gcgtgcgacg aatcgaggct gtaacaagta
    23041 aagaagcgta cgaactactt gaagaagaag aaaaacaact aaaacaagta gcaacaattg
    23101 ttaaatcacc tcaattgaaa gaagttgttt caaaagcgga acaattgcaa cagcaagtaa
    23161 gagagctaca aaaggaaaat gaacaattag ctagcaagtt agcgaatcaa caagctggtg
    23221 atgtattcaa agatccagaa acgatcaatg gaaaaacaat cattgcagca caagtaaaag
    23281 tgaaagatat gaatcagctg cgtcaattag ctgaccaatg gaaacaaaaa aatatttcag
    23341 atattcttgt tttagcaaac gaacaagaag gaaaagtcag tctgctagcg gcaatgacga
    23401 aagaatcaaa tgaagctgga ctaaaagctg gcgacttgat taaagcaatc gcaccgaaag
    23461 taggcggtgg cggtggtgga cgtccagaca tggcacaagc tggcgggaaa aatcctgctg
    23521 gtattccaga tgcacttaaa gctgcgaaag aatgggttgc gcaacaatag ggcgtttacg
    23581 tttgacagaa aaacaaaagt tcaaacattg aacgaaacat gattatgggg gcgaagacaa
    23641 acaagtcttg ccctcatact tttttcaatt ggatggcgca tctaatcaaa tggtcggatt
    23701 cggatggtct ttagttgacg aaagtgacag aaatttttac gattagtata aataaaaagg
    23761 ggtgaaaaaa tgaatagtag aaataagacc tttcgactag tcttacgtgc gatcttgtta
    23821 gcaatcatca tcgtacaagc gatggttcct tggctggggt ttattccatt aggattcatt
    23881 agtttaacaa ttatccatat cacagtaatt attgctgcag tagtattagg cccaaaagat
    23941 ggcatggtga ttggcttgtt ttggggaatc gttacaattg ttcgtgccta tgccatgcca
    24001 acaacgccat ttgatacatt agtgttcacg aatccgatta tctcggtagt tcccagagta
    24061 cttgttggct tagtagcagg cttggttttc cactggattt atcaaaaaaa taaatcaata
    24121 acgatcagct cagcctttgc aggggtactg ggttcattgg tcaatacagt cttagtgtta
    24181 ggattcatgg gattatttta tacaggtgca acggcagatg cctatggtgt agactctgct
    24241 cttttattta aaaccttagc aggggttgca gcaatcaatg ggataccaga agcaataggt
    24301 gcgggaatca taacaccatt actagcaaaa gcgttatttg cagcaacacc gttgaaacca
    24361 gaataatgac catcttttaa tagtaatagg acgcacgata gatagaaaaa ggaagagagg
    24421 agaggaaaac aatcgtcctc tccatctttt tacctgagca acaagcaaac tagaaggggt
    24481 aaaaaacttg gctaagaaac aagaagaaaa aaagaaacaa ataccgcaac agcggacaaa
    24541 aaaaggagta ctagcgggta tcttaggatt ggttacgaat ctgatcttgt ttgtctctaa
    24601 gctaatgatc gggctttttt caggaagtgt ctccattatg gctgatgcag tgaacagtct
    24661 atcagatact gcttcatcca tattgacttt gatcggattt aaaattgctg caaagccagc
    24721 agatcaggaa catccttatg gacacgaacg cttcgaatat atcagtgggt tatttgtttc
    24781 gatcatcatt acttatgtag gatttcaatt tttagagtct tctgctgaaa aaatcatcca
    24841 tccagaaaat gtctctttaa cacccatcgt cttttttgtc ctaatcattt ccatcttttt
    24901 gaaatttgca caaggcaaaa tgtatcaggt tattgcgcaa tccattgatt ctgaaacatt
    24961 gagagctaca gcaacagata gttataatga tgtctttact acactggctg tattgctttc
    25021 agcagcaatc gaatgggtaa ctggatggcg tattgatgga tatataggtt ttgctttagc
    25081 attatatatc ctttatagtg gattgacgat gcttcgaaat tttgtatacg aactattagg
    25141 aagtcgccca acgccagcag agattgaaga aatggaagag catttaaatg cttgcgatga
    25201 tatacttggc tatcatgatt tattggtgca taactatgga cccaataaac gctttgcatc
    25261 agtccacatc gaagtagatg aatcaatgaa tttgaatgtg gctcataaga ttattgatct
    25321 gatagaaaaa gactttaaaa agtctttgga tgtggaactt gtctgtcact tggatccaat
    25381 gcctgttgat aacgaaaaat actataatat cttgcggcag ttaaagcaaa ttcttcttga
    25441 aacaggtaca gggcttaata ttcatgattt tagagtaatt catcaaggaa aaacgctgca
    25501 atttgatgta gtggtccctg agaaattcca gtttgttgat acagaactag aaagaatctt
    25561 acgtagtcaa atagaagaaa aaatcggaga ttaccaattg gaaattacgt ttgatcacaa
    25621 ttatctttta tattaaatga gaaactataa aaagactcct taaagttttt aatttaaaga
    25681 gtcttttttt cttttaattg tttatggaga gcctgacgtg caagattatc tgctccttta
    25741 tttttgtttt cgggcaacca ttgcaggatc aataaagaaa attcggatag aagttcttgg
    25801 attctttgaa ggtagggaag gaagagctcg ttcttcgttg cgtttttgtc gaccgtagaa
    25861 actagtgttt tgctgtctga aaaacagaaa atcgtttcat tctgtaagtc gtttttcttt
    25921 agaaattcta aagtttttaa aaagacttcg aactctgctt gatggtttgt ccctatagaa
    25981 agcgggatag atagctgttc ttgtttacct tgatagacaa tcaagattcc acctccacta
    26041 ggaccagggt tgccttttgt agaggcatca atgtatactt ttaacataaa aagagattct
    26101 cctttttgtt caaactaatg ttatggtaag attatatcat tactgaatcg gtgagaggtt
    26161 agagaatgaa aaaaaatatt cgatggcagc ctgaactggc acatacgatc atctattggt
    26221 cattgacctt gatcattctg ttttttagtc tgattctttc tttagaaaac acaggtcctt
    26281 attggaaaag caattttgtt ttaggtattt ttcttgtttt tctatttcta ggtttgcggc
    26341 gttcttttat tttaaaatca aactctgtga ttatacgtta tgctgctttt tggaaagacc
    26401 gagaaatatc tgtcggacag attcgatctt tgacgccaac taatactggg gtgcaaatga
    26461 tgttgcaaga tgagaaagcc ccatctgttt tcatgatgcg aaaaaaagat caagtagtcc
    26521 tttttgatca ttttgaaaaa aacggaccca aatcagaatc aattccttat ccttatacga
    26581 aaaaaagaaa aagctaattt taaaaatcag aacctaaaaa aacgaatttt tacaggaaaa
    26641 gaaaagagtg agacgtacgg ttcatcccgt ttagcgtctc actctatttg tttatctatt
    26701 catgcatact agtatcggta gtctgaacgt tgcctttctt gacatgggca gcttgtgttc
    26761 ctcgattccc ttcgatgatc tcaaattcga ccacttgatt ttcttccaaa gttttgaatc
    26821 cctcttcttc aatcgcagta aaatgtacaa aaatttcttc tgtgtcatca taactgataa
    26881 agccgtatcc ttttttgttg tcaaaccatt ttacaattcc tgtggtcaaa gcactcacga
    26941 cctttctaaa gtctgatcat agccttcatt ataaagagta tttataagta cgtcaaataa
    27001 attaaaaacc tgtaatttta tgtttttttt ctgaataaaa aaaggtataa ttggcatatc
    27061 ttaacaaaga agatattttg ttgaataaag ttcgtgaaaa ggggtaatca aatgaagaat
    27121 gatctgcaaa tttcacaaga aacagagcta gaaccgattg ttgaagtggc tagtaaatta
    27181 gatcttaagg aagacgattt ggaactgtat gggaaatata aggcgaaaat caatttttct
    27241 ggaatcaatc gatcaaagga taacgctgag gggcatttga tcttagttac ttccatcaat
    27301 ccaacaccag ctggcgaagg aaagtcaacg atcactgtag ggttaggaga cgcgctcaat
    27361 aaaatcaata aaaaatctgt tattgcattg cgagaaccct cacttggtcc tgtgatggga
    27421 atcaaaggtg gtgctgcagg cggaggatat gcacaggtat tgcctatgga agatatcaac
    27481 ttgcatttta ctggtgacat gcatgctatc acaacagcta ataatgcatt atctgctctt
    27541 ttagataatc atattcatca aggaaatgaa ttgaacatcg atgcccgccg agtgatttgg
    27601 aaacgggtag tcgatctcaa tgatcgtgaa ctgcgtaaag taattgttgg cttgggtggg
    27661 ccaatccaag gtgttccaag agaagatggg tttgatatca cagttgccag cgaaatcatg
    27721 gctatcttgt gtcttgcggc agaccttgaa gatttaaagg ctcgtcttgc aaggatcgtt
    27781 gtcggctata catttgaccg tcagcctgtg actgctggag acttgaaagc agaaggcgct
    27841 ctggcattac tgctgaaaga tgcaatcaag ccgaatcttg tacaaacaat ttacggaaca
    27901 ccagcttttg tccacggggg accgtttgcc aatatcgctc atggctgcaa tagtatttta
    27961 gctaccaaga cagcattgag attagcagat tacgttgtca cagaggcagg atttggtgca
    28021 gatcttggcg gagagaaatt tttagatatc aaagtaccta acttgaaaaa agcgccggat
    28081 gctgtggtga ttgtcgccac tgtccgtgca ttgaaaatgc atggcggaat gaaaaaagat
    28141 gaacttaaaa atgagaatct tgatgctttg aaagtaggtt ttgcaaattt aaagcgccat
    28201 atacgtaata tggaacaata tcagctacct gtgatcgtcg caatcaatga attcgtaaca
    28261 gatacagaca atgaactcac actgttggag cacttgtgtg aaaatcaggg gattcttgcc
    28321 aagagagcga gtgtatgggc agatggagca gaaggcggcg ttgatttagc aaaagctgta
    28381 gtacgattga ttgatagaaa agaagctaat tacaagccat tataccgcct agaagaaacg
    28441 atacaagaaa agacagaaac aatcgtcaag aaaatttacg gaggaaatgg tgttgttttc
    28501 agcaaaaaag cgcaaaaaca aatagaagag tttacgaaga atggatggga caaactgccg
    28561 atttgtatgg caaaaacaca gtattcattt tctgatgatc cttcactgct aggtgcaccg
    28621 gaagatttca ccatcacgat ccgcgaaatc atcccaaaat taggggcagg atttttagta
    28681 gccttgactg gagatgttat gacgatgcct ggtttgccta aaaaacctgc tgcattgaat
    28741 atggacgtga caaatgacgg agaagtgttg ggattgtttt aagcttgatt ataatctacc
    28801 atcgaaggag ctgtattttg actacagctt ctttttttag acgataggga attaattttg
    28861 tttacactag gatcattctt tttctttatt gtattttgag ctatactaaa gcataatagg
    28921 agggtatgat gaaaacgaca tttataagaa aaaccggttt ttacggtatg ggtagctcga
    28981 ttcagctaag aatcaacaat gaaaaacgca gtgttctcag tcatgaacaa aagataacgc
    29041 ttgatctgga agtgcccttt acgatgcagg tcacctttta ttggctaaaa agccccgttt
    29101 atacagtgaa agaagcaaag cctgcttatg tgatcacaat gaattacttg attcttcaaa
    29161 tctatccgtt tttatttcta gcaactgggt tgggggccgt tttcctacaa aatttatttt
    29221 tcagtttttt tgcagttatt gtgttgattg cgttcttttt ctatataaaa aaccgggcat
    29281 atttgatcaa ggaggaaaat tatggcaaac tctgatcttt ttttaatcag tttcaatcga
    29341 atcgaaaaat ggatgagaga cgagatggga aatgcacgaa atatgggatt taccgaactt
    29401 gtcagacgcc tagctcaaag aaaacaatta atgatcagaa aatacgaaga tgatctttta
    29461 cagcttgctc agttaaggaa tgcgatcgtc catgatcgga tcgcagtcga ttttgtcatt
    29521 gcagagccaa atgattggac agtcaagcga atccagctga tcgaacaaga actgattcag
    29581 cctgaaaccg tgctgcctcg atttgcaaaa cgtgtaacag gttttgaaca agatcttccg
    29641 ttacttgaac tgttaaagat agtagcagaa aaaaggtatt cacaatttcc gttatacaat
    29701 aaaggaaaat ttgtagcatt gattacttta cgtaatatcg ggttttggct agcaaaagaa
    29761 agccaaaaag gtccggttga tttaacaaat aaacgagcgt tggatttgat catccaaaac
    29821 ggaaagtaca caaattatca ttttgttcca gctaaaacac atatttacga ggttgaagcg
    29881 atgtttcgtg agcaagggac tttggaagcc gtacttatca ctaaagatgg gaatcccgat
    29941 ggtaatctgc tcgggatcgt acgacctcgt gatatttata aacaaataga aaaggattga
    30001 ttttttgtta attatttatt ggatattcag cgctttgcta gtaggagtgg atcagttggt
    30061 caaatggtgg atcactgata atctagcgct tggagagacg aaaaatctta taccaggtat
    30121 tttgtcactg aatcatattc gaaacactgg agccgcatgg agtatgctag aagggaagat
    30181 gtggttcttt actatagtga cactcattgc agtcgttgtg attctcacat tgatgattaa
    30241 aaatcgtgaa aaaggtaatc gctggttcat gattggattg agtttgattc tagctggtgc
    30301 gattggcaat tttatcgacc gtattcgtct tggctatgta gtagatatgt tccagacaga
    30361 ttttatgagc tttccgattt ttaacgtagc agatgtgaca cttgtatgtg gagtgatttg
    30421 tatgttaatt tatattattt tagatgaaaa ggatcaaaga aaaaaatgac agaacttaaa
    30481 acgacgattc accatgaatc tggtaggatc gataaagtat tgactaattt attcaccgat
    30541 tacagtcgtt cgcaaattca gctgtggctg aaaaatggag cggtttctgt caatggacag
    30601 ccagtcaaag caaattacaa agtaaaacaa ggagacgaga ttactgtctt gatccccgaa
    30661 ccagaaacat tagatgtggt cccagaaaat atcccgttga cgatcgttta cgaagatgaa
    30721 gatgtagcgg tgataaacaa acctcaagga atggtcgttc atccttcagc aggtcatacg
    30781 catggcacaa tggtcaatgc gctgatgtac catatcaagg atctttctac gatcaacggc
    30841 gttattcgtc caggtattgt tcaccgaatc gacaaagata cttccggact gctaatggtt
    30901 gccaaaaatg accaggcaca cgaatcatta gctcagcaat taaaagataa gacctctttg
    30961 cgcaaatatg ttgctttagt ccatggagag attcctcacg aaaaaggaac catcaatgcg
    31021 ccaatcggcc gttcgaagga aaaccggaaa atgcaggcga taagagaaga tgggaagtcg
    31081 gctgtcacac attttactgt attggaacgt ttcgaaggat ttactctggt tgaattaaaa
    31141 ctagaaaccg gaagaacaca ccagatccgt gtgcatatga agtatatcgg ttatccgtta
    31201 gctggagatc cagtatacgg accgaagaaa actttgaaag gcaatggtca gtttctccat
    31261 gcgcaaatgt taggttttac gcacccgact acaaacgaaa agatgatctt tgaagcacct
    31321 cttccagaag tttttgaaaa aactttagaa aaattaagaa aaaacattgc attttaattt
    31381 agataacggt atagtaatta acaacgaaag cataaaactt aatagaattc ctttaagttt
    31441 agtcccgtga ggctaagaag ggtgggagca aggatacgta tggtgcgcca taagtagatt
    31501 gctcattgac atagtatagc tatcagtgta tcagtatacg ctcagtctaa acattcctcc
    31561 tgtcctctta gggcaggagt ttttttatgc aaaaaagtag ggggaatgaa aatgcaagca
    31621 aaagaagtag tagatcaggt aaccatgaag cgagcattga ctcgaattac ttatgagatt
    31681 atcgagagaa accatagtat ccaagatatc gtattggtag gtatcaaaac acgaggaatc
    31741 tacattgcat caagaattgc cgaacgcctg aagcagctag aagatatcga tattcctgta
    31801 ggagaactgg atatcacttt ataccgagat gacaaaaaag aaaatccaga agaaccggaa
    31861 cttcattctt ctgacatccc agtttcctta gaaggaaaag aagttatttt gattgatgat
    31921 gttttgtata caggacgaac gattcgagca gcaatggacg cagtaatgga ttttggcaga
    31981 ccaagaaaaa tttcattagc agtcctggtc gatcgtgggc atcgagaatt gccaatccgt
    32041 gcagattatg ttgggaagaa cattccaaca tctcgggcgg aagagatttt agtagagatg
    32101 caagagcttg atggacaaga tcgcatcatg attttgaaag aggaagatta gaggagagag
    32161 aaacatgtcg gagaaaaaag gatttcataa tgatgacgtg gtgttagata tccacgatcg
    32221 cccaacccca ggacattggg taggattaag tttacaacac ttgttcacga tgttcggagc
    32281 aaccgtgtta gtccccatct tagtaggtat cgatccaggt attgcgctag tcagttcagg
    32341 actaggtaca ttggtgtact tatttactac taaaggaaag atacctgcgt acttaggaag
    32401 cagcttcgct ttcattgcag cgatgcagct tttgatgaaa acagatggtt atccagcgat
    32461 tgcacaagga gcagtcacaa ctggattagt ctatttgatt gttgcgctga tcatcaaaaa
    32521 gatcggttca gcatggctgg ataaaatctt gcctccaatc gtcgtcggtc cagtcatcat
    32581 ggttatcggg ttgggacttg cggcaaatgc agcaacaaat gctatgtata atcagggcac
    32641 gtatgatttc aagtatattg ctgtagcgct gatcacatta ggattaacaa ttttcttcaa
    32701 catgtggcta agaggttttc tcggattgat tccgatttta ctaggtatta ttagcggata
    32761 catcgtagcc ttgctgtttg gaatcgtgga tactaagcca atcgtagacg ctgcctggtt
    32821 tgctgttcct aactttgaga ttccctttgt acaatatgaa ccaaagctat atcttggagc
    32881 aatcacaaca atggcaccca tcgcttttgt gacgatgaca gagcatatcg gccatcttat
    32941 ggtattgaat aaactaacta aacgtaattt ctttgaagaa ccgggtcttc ataagacgtt
    33001 gacaggtgat ggattagcac aaatcgtggc agcctttgtc ggaggtcctc ctgtaacaag
    33061 ttacggtgaa aacattggtg ttctggctat cacacgtgtc catagcgtgt ttgtgatcgg
    33121 tggagctgca gtatttgcag tcattttagg attcgtagga aaattaagcg ctttgatttt
    33181 aagtatccct ggaccagtca tctcaggaat cagttttatc ttatttggag taattgccgc
    33241 aagtggaatg aagattttaa tcgagaacaa aatcgatttt gataaaaaga aaaatctgtt
    33301 gattgcttca gttatcttag tagtaggaat tggcggactc gtctttgaag taggcacatt
    33361 tacattgtcg gcaatggcat tagcaactgt acttggcatc attttaaatc ttatcttacc
    33421 agaaacttca cgaagcgaag aaaactaagg aggaaacatt atgatcatca aatcagaacg
    33481 tatcagctta aagcatttat tgaccgttga ggcacttact gatcaagaag ttatgggctt
    33541 gatccgcaga gggcaggaat tcaagcaagg tgctaattgg actccgcaaa agcgccagta
    33601 ctttgctaca aacctctttt tcgaaaatag tactcgcaca cataaaagct tcgatgtagc
    33661 ggagaaaaaa ctagggctag aagtgattga atttgaagca agcacaagct ctgtccaaaa
    33721 aggggaaaca ctatacgata cagtactgac gatgtcagca ttaggcgtag acgtagcggt
    33781 tatccgtcat ggtgatgaga actattatga cgaactgata caaagtaaaa caatccagtg
    33841 ttcgattatc aatggaggag acggaagcgg acagcatcca acccaatgct tgcttgattt
    33901 gatgaccatt tatgaagaat ttggtcattt tgaaggtctg aaagttgcca tcgtaggtga
    33961 tatcactcac tctcgcgtag ctaaatccaa tatgcagatg ttaaaacgtc taggcgcaca
    34021 agtattcttc tcaggtccta gagaatggta cgacgatgaa ttcgaagtgt acggacatta
    34081 tatgccattg gatgaactac tagatcaagt tgatgtcatg atgatgcttc gggtgcaaca
    34141 cgaacgccat gatggaaaag aaagtttctc aaaagaaggc taccatcagg aacatgggtt
    34201 aacagttgag cgtgcgaaga agttgcaaaa acatgcaatc attatgcacc cagcaccagt
    34261 taaccgtgat gtagaattag ctgattcatt agtggaagga ttacagtcgc ggatcgttca
    34321 gcaaatgagc aatggagtat tcgtcagaat ggcaatctta gaagcagtgc taaatggaaa
    34381 agcgtaatta taggaggatg aaaaaatgaa aacactcatt aaaaacggtc agatcaatac
    34441 acgaaaaaat atgacgacac cagcagaaat ttggatagaa gatgggcgaa tcaaagcaat
    34501 cggtacagga ttctcagaag ccgaatttga tgaagtgttt gatgcaaagg gacaattgat
    34561 cacacctggt ttagtggacg tccatgttca tttgagagag ccaggattta catacaagga
    34621 gacgatcgaa gctgggactc gctcagctgc tcgtggcggt ttcactactg tatgcgcgat
    34681 gccgaattta aatcctgtac ctgatacggc agaaaagctg agacaagtat atgacatcat
    34741 tagaaaagat gcagttgtga aggtattgca gtatgcgcca atcacagaaa atctgcgaag
    34801 tgagaaatta gttgatcaag aagcactgat tgaagaaggt gcttttgctt tcacaaatga
    34861 cggtgtaggt gtccaaacag ctgggacgat gtatctggct atgaaagaag ctgcaaaaaa
    34921 caataaagcc ctagtagccc atacagaaga tgaatcttta ttatttggcg gcgttatgca
    34981 cgctggcaag aaggcagaag aacttggtct tcccgggatc ttaagcgtaa ccgagtcttc
    35041 tcaaattgct agagatctgt tgctagctga agcaactggt gttcattacc atgtttgcca
    35101 tgtttctact aaagaaagtg tccgtgtaat cagagacgca aaaaaagcag gaatccacgt
    35161 aacagcagaa gtttcacccc atcatcttat tttgatcgac gaagatattc ccgaagattt
    35221 cggcttttgg aaaatgaacc cgccgctaag aggacgggag gacagagaag ctttgatcga
    35281 aggcttgctt gacggtacga tcgattgcat cgctacagac catgcccccc atggattaga
    35341 agaaaaaagc caaagtttca tgaaatcacc atttggtatc gtaggaagcg aaacagcgtt
    35401 tcaactgatc tatactcatt ttgtagaaac tggtcgattc acgttggaac aagtgatcaa
    35461 ttggttggca gttaaaccgg ctgagatttt tggtttgaat gcaggaacgc taacggttgg
    35521 tgcccccgct gatgtagcag tttttgatat tacacaaact tgcacgatcg ataaagaaga
    35581 ctttttatca aaaggggaga atacaccttt tatcggctgg aaagtaaaag gcgaaacaca
    35641 aatgactttt gtcaacggaa aacttgtgtg gcagaaggga gaataaaagt atggaacgtt
    35701 tgcttatatt agaagatgga acagttttcg aaggaaaggc ttttggtgca gatgctagtg
    35761 tcttcggaga aatcgttttt acaacaagta tgactggtta tcaagaaact atcactgatc
    35821 aaagttttaa cggtcaaatc attacattta cctatcctat ggtgggtaat tatggggtaa
    35881 atagagatga ttatgaatcg atttctccaa catgcaaagg agtagtagta aaagaacatg
    35941 cacgtgtagc ttcaaactgg cgaaaccaaa tgacgctaga ttcatttttg aaacaaaaag
    36001 gaattcctgg aatatcaggt atcgatacgc gcgcccttac tcgcaaaatc cgtgaagtcg
    36061 gaacgatgaa agctagtata gttgatgctg gagattcctt cgaacatgca tttgatcagc
    36121 tgaaagcaac cgttctagca accaatcaag tgcagcaagt ctcaactaac aaaccttatc
    36181 caagtccagg aacaggtcgg aaagtcattg tcattgactt cggcttgaaa catagtattt
    36241 tacgagaatt gagtaaaaga aactgtaacc tcacagtact tccctataac acagatgccg
    36301 agacgatttt agcgctatca ccagacggag tcatgctgac gaatggacct ggtgatccaa
    36361 aagatgtgcc agaagcaatc gaaatgatca aaaaaatcca aggaaaagta ccgatttttg
    36421 gtatttgtct cggtcaccag ttatttgctt tagccaacgg tgcagatacc tataaaatga
    36481 aattcggaca tcgaggatta aatcacccgg tacgagaagt agcgaccgga agaattgatt
    36541 ttacttctca aaatcatggc tatgcagtcg atgaagccac gattgatcca gaaaaactca
    36601 tcgtaactca tgtagaagta aatgacggaa cagtagaagg agtcaaacac agagattatc
    36661 ccgctttcag tgtgcagttt catccagatg ctgcccctgg tccgcatgat gcactacatt
    36721 tatttgatga atttatggaa atgatggacg caggaaagga gcagtaaaca gatgccgaaa
    36781 cgtacagata tcaagaaaat tatggtgatt ggatctggtc cgatcatcat tggtcaagcc
    36841 gcagaatttg actacgcagg aacacaagcc tgccttgcct taaaagaaga aggatatgaa
    36901 gtggttttag taaattctaa tcctgcgacg atcatgacgg ataaagaagt ggcagatcaa
    36961 gtatatatcg aaccgatcac acttgaattt gtatcaagga ttcttcgaaa agagcgtcca
    37021 gatgctctcc ttccaacttt aggcggacag acaggcctca atatggcaat ggagctggca
    37081 gcatcaggta ttttagaaga attgaacata gaacttctag gaacaaagct ttctgcgatc
    37141 gatcaagcag aagatcgtga tctattcaaa cagttgatga aagaattgaa tcagccaatc
    37201 cccgagtctg aaatcgtcaa tacagtagaa gaagctgtag attttgcaaa taaaatcggc
    37261 tatcctgtca tcgttcgtcc agcctttact ttaggcggaa ctggtggagg tatgtgtgaa
    37321 aatgaagaag aattacgtat cgtatcagaa aatggattaa gtctttcccc cgctacccaa
    37381 tgtttgattg aacgaagtat tgcaggtttt aaagaaattg aatatgaagt gatgagagat
    37441 tcggctgata atgcaatcgt tgtctgcaat atggaaaatt ttgacccggt gggtatccat
    37501 acaggtgatt ctatcgtatt tgcccctagt caaacattgt ctgaccatga atatcaaatg
    37561 cttcgtgatg cttctctttc gattattcga gcacttaaga tcgagggagg ctgtaatgtc
    37621 caactcgctc ttgatccaca tagttttaat tattatgtga ttgaagtcaa tccgcgtgtt
    37681 tctcgttcat ctgcgttagc aagtaaggcg actggctatc cgatcgctaa gctagctgct
    37741 aaaatagcgc ttggattaac attagacgaa atgaaaaatc ctgttacagg aacgactttt
    37801 gcagaatttg aacctgcatt agattatgtt gtagctaaaa ttcctcgctg gccgtttgat
    37861 aagtttgaaa acggagaacg gcttttaggc acgcagatga aagcaactgg tgaagtgatg
    37921 gctatcggtc ggaatatcga agagtcttta ttgaaagctg tgcgttcttt agaaatcggt
    37981 acacaccata tcgaacttcc tgagttggca gacatcacgg acgatgacct aatccaaaaa
    38041 gtggttcgag cacaagatga tcgcctgttt tatgtagctg aagctattag aagagggtac
    38101 accatagaag aattagcgga tttgacaaaa atcgatttat tctttttaga taaactgttg
    38161 catatcgtag aaatcgaaca gcagttgaaa cagcagccgt tggatgacaa gatattgaaa
    38221 acagcgaaac aaaacggttt tacagacaaa aaagtggcgg agctgtggga aataaacgaa
    38281 gaagccgttc gccagatgag aaaagaaaat gatttgattc ctgtatataa gatggttgac
    38341 acttgtgcag cggaattcga atcaaaaaca ccttattttt atagttccta tgaatatgaa
    38401 aatgaaagtt cacgatcaga aaagccctca attctagtac tgggatctgg tcctatccgt
    38461 atcggacaag gggttgaatt tgactatgcg actgtgcatt ctgtcaaagc aatccagcag
    38521 gcaggatacg aagcaatcat catgaacagc aatcctgaaa cagtttcgac tgacttttct
    38581 atttcggata aattatattt tgagccattg accttcgaag atgtgatgaa tgtgatcgag
    38641 ctagaacagc cgataggagt aatcgttcaa tttggtggtc agacagcaat caacttagcc
    38701 gaacctttga ctgctgctgg tgtaaaaatc ttagggacaa cgatcgaaga cttggatcgt
    38761 gcggaaaatc gtgatttatt cgagcaagcg ctgaaagagt tggatattcc tcaaccttta
    38821 ggggatactg ctacaagtaa agaagaagcc gtggttatcg cttcgagaat tggttatcca
    38881 gttcttgttc gtccaagcta tgtattaggc ggacgtgcga tggaaatcgt tgaaaaccag
    38941 cgagatttag aagattacat ggaacatgct gtgaaagcat ctccagaaca tccagtacta
    39001 gtggaccgtt acttgcttgg tggtgaatgc gaagtggatg cgatttgtga cggtgaaaca
    39061 gtactcatcc cagggatcat ggagcatatt gagcgtgctg gtgtccattc aggggattca
    39121 atggctgtgt atccacctca aacattatct gaatcgatca agaaaacgat cgaagattac
    39181 acgatccgct tagcattagg tttgaattgt gtcgggatga tgaatatcca gtttgtcatc
    39241 catgaaaatg aggtttatgt gatcgaagtc aatcctcgag caagtcggac tgttccattc
    39301 ttaagtaagg tgacaggaat tccaatggca caaattgcga cgaaagctat tttgggagag
    39361 aaactaaaga cacttggtta tgaaaacgga ctttatccag aaacaaaaga agttcatgtt
    39421 aaagcacctg tcttttcttt tacgaaattg cagaaagttg atacttactt agggcctgaa
    39481 atgaaatcga ctggagaagt aatgggatct gacagacaat tagacaaagc tttgtacaag
    39541 gcattcgaag cttctggatt acacttgcct gaatatggag ccgtattatt tacgattgcg
    39601 gacgaaacaa aagaagaagc actagaatta gctaaacgtt ttacagaaat tggctatagt
    39661 ctgataggaa cgaaacatac agctgcttat ttcgaaaaaa atggtctggt cgttgaaaca
    39721 gtagcgaaaa tttctgagga agcagcagaa aaaaatgtag tcgacttaat aagagatggt
    39781 aaaacacaag tggttgtcaa tacgattgat aaagatcggg gaaatgcttc taaagacggt
    39841 ttcatcatcc gaagagaagc agttgaacat ggtattccat tgtttacttc tttagacaca
    39901 gcagatgcga tcgttcgagt gatggaatcc cgtgcttttt caactaaccc tatttaacgg
    39961 cgagcacatg ttaagacaaa aagacgaaaa accgttgcag gaggggacag atgaaacaag
    40021 aaatgatgga aatcgttcag caaaaacagc tggcaccaaa gatttttgaa atgacattga
    40081 aaggtgatct agtaaaagaa atgcagcaac caggacagtt cttgcatatt ctcgtgccgc
    40141 gtgcagactt gctattacgc cgaccgatta gcttgaatca tatcgaccac gaaacaaata
    40201 cttgccgtat catttatcgg gtggaaggtg aggggactag agtcttctct actctttctg
    40261 ccggagacca tttagatgtc atgggtcctt taggcaatgg ttttgatttg tccaatcttc
    40321 atacaggaga cgaagtattt ataatcggtg gaggaatcgg tgtccctcct ctatatgagt
    40381 tatcaaaaca gttagtcgaa aaaggaatta agccaacgca ttttttaggt tttgccacac
    40441 atgaagtcgt ttattatgaa aaagaattta atcgtctagg agaaacaaga atcgctactg
    40501 acgatggaac atacggtatg cacggaaatg tcggtaattt attgttagga acgaagacgg
    40561 aacctacagc tgtttttgca tgtggttcaa acgggttatt gaagacagtg gaacagctgt
    40621 tttcttcgca tcaaaatgtc cagttatcat tagaatcccg catggcatgt gggatagggg
    40681 cttgctatgc ttgcgtttgt catacgccgg aagatgaaac aggaacaaaa agtgtgaaag
    40741 tttgtgacga aggaccgatt tttaaaatag gagaggtaat tatatgaatg agaatccttt
    40801 agcggtcaaa cttcctggcc tagaattaaa aaatcctatc atgccggcaa gcggctgttt
    40861 cggttttggc aaagaatacg gaaaatacta tgatttgaat cagcttggat cgatcatggt
    40921 caaggcaacg actccaaatg cccgctttgg caatcccacg ccaagagtgg cggaaacacc
    40981 atctggcatg ttgaatgcaa ttggtttgca aaatcctggt ctagacgttg ttatgcataa
    41041 acttcttcca gaattagagg agtacaatga gttacccatc atcgcaaatg ttgctggtgc
    41101 ttgtgaagaa gactatgtag aagtttgtag caaaattggc gaagcaccaa atgtgaaagc
    41161 aatcgagctg aacatttctt gcccaaatgt caaacatggc ggaattgctt ttggaacaga
    41221 ttctgatgtt gcttttcaat taacacaagc agtaaagaaa gtttcttctg ttcctgtcta
    41281 tgtcaaattg tccccaaatg taacagacat cgttccaatc gctcaggcaa tcgaagctgg
    41341 aggggcagat gggtttacga tgatcaatac tcttcttggt atgcggattg accttaagac
    41401 aagaaaaccg attttagcca atcaaacagg tggactctcc ggaccagcaa tcaagcctgt
    41461 cgctattcgt ttgattcacc aagtagcaag tatatccagc cttccaatca ttgggatggg
    41521 cggcgtgcag acagtggatg atgttcttga gatgtttatg gctggagcaa gtgctgtagc
    41581 tatagggacc gcgaatttca ccgatccgta tatctgtcct aaactaatta aagagctccc
    41641 tgtgcgaatg agcgaactag gcatcgaatc gctggaaaga ttaatcaaag aagtgagaga
    41701 ggaacgtgaa cgatgaatca actagaccaa cgaccaatta ttgcattaga tttttcaact
    41761 aggcaagagg tagaagactt tcttcgattt ttcccgaaag aagaaaaatt gtttgtgaaa
    41821 atcggaatgg aattgttcta ccaagaagga cctgaaatcg tacgttatct aaaagaggct
    41881 ggtcataatg tatttttaga tctgaaactt cacgatattc cgaatacggt cgaaaaagca
    41941 atgcggggat tggcaaagct tggtgttgat ctcacctgtg ttcatgccgc tggcggtatt
    42001 cgtatgatgg aggcggcaat gcgaggatta gaagagggga ctccagaagg tggaaaacgt
    42061 ccgctacttc tagctatcac tcagcttact tctacaagtg aagaagaaat gcatgccgat
    42121 caactaatcg aagtttcttt agaaaaaagt gtgatccatt atgctagctg tgccaaaaaa
    42181 gcaggattag atggtgtggt ttcttctgct tgggaagtag aagcgatcaa agaaatggca
    42241 ggagacgaat ttgtttgctt aactccgggt ataagaccag aaggaacagt cgcaggagat
    42301 cagacaagag tagtcacacc ttctcaggca aaaaagatcg gctcaacttt tatcgttata
    42361 ggtcgaccaa tcactcagtc aactaatcct tataaagcat atcaaacgat tcaaacagaa
    42421 tggagctaac ctaaaatgaa tgttgaacaa agtatcgcaa aagacttatt agaaattgaa
    42481 gcagtatttt taaatccaag taatcctttt acatgggcaa gtggaatcaa aagcccaatt
    42541 tactgtgata accgaatcac gatgagctat ccaaaagtcc gaaaagagat cgcaaaagga
    42601 ttagcaagca aaatcaaaga agcttaccct gaagtacaag tgattgctgg aactgcaaca
    42661 gcaggaatcc ctcatgccgc atgggtagcg gaaatactgg atttgccaat ggtctatatt
    42721 cgaagtaagg cgaaagatca tgggaaaggt aaccaaatcg aaggacgtat atttgaaggt
    42781 cagaagatgg ttgtgatcga ggatttgatt tctacaggcg gaagtgtctt ggaagcagct
    42841 gaagcagcaa aaagagaagg tgcggatatc cttggtgttg cggctatctt tacgtatgag
    42901 ttgccaaaag gaaaagcaaa tttcgaaaaa gccgaaatcc ctcttatgac gttgaccaat
    42961 tattctgtat tgattgaagc agctttagaa gatcgttata tcgatgagca agaattaact
    43021 ctgctaaaag aatggaaaaa agatcctgaa aattggcaga tttaagcgcg cttgatgaag
    43081 atagagaaat ccgatttgtg tctccagaat catcaattgg aatagcaaaa atccaaaaac
    43141 tgattgcgag aagcatcttt tcatcaggta tgaaaagaaa cttctatcgt gatcagtttt
    43201 tgattttttt aaaattcatt tttctaagac tggttagcag aaagaagaga ataatctctt
    43261 ctcttttttc tattatatat agattttctc tattttttgg taagggagct gtttcaaaaa
    43321 tggtaaagag cggaaatgtt gcgaatatca gttatttaat gatatgatat tcggaaaaag
    43381 aaatctcaaa gggaggtaac ttttatgggg ttgaatgaat ttattttagg attgaacaat
    43441 ttagagacga tcacaagggc acctggattt tttaaatata cagagcatac agttgcagca
    43501 cattcgtatc gtgtagcatc tattgcgcaa gttctaggag acattgaaga agcgaatgga
    43561 caaccgatcg attggaaact gctctatgaa aaatcattga atcatgacta cacagagcgt
    43621 tttattggtg atatcaaaac gccggtgaaa tacgcaagca aagagttaag agggatgctg
    43681 caaacagttg aagaaaagat gacggacgaa ttcatcaacc aagagattcc agctgagttc
    43741 caagaagttt atcgccgtcg gttgttcgaa ggaaaagatg aaaccgtaga aggagaacta
    43801 ttagccattt cagataaggt tgaccttctt tatgaatctt ttgatgaaat catcaaaaat
    43861 aacccggaaa aagtttataa ggagatgttc attgaggcag tgactacgat taaaagcttt
    43921 gaacatcggc cttctgttca gtatttcttt aaagagatat ttccggaact attgaaccag
    43981 gaattttatg gaaaagaggc gtttttagaa accgtctcaa cgattgtaaa gaaagaagaa
    44041 aaataaaaat gatatgagat gcagggggaa gtatagttga aaaagaatat ggatgtcgaa
    44101 tggggctatt ctggagacaa aggtccggaa aattgggcga gtttatgtga ttggtttgcg
    44161 agaggtgcgg aatttccatt gcagtcgcct attcatttga ccaaggggga agaaaccaaa
    44221 atagaaggag acacgctttc ctttcattat caaaagcaac ggtttactga caaggaattc
    44281 aaaaacacga ttcatttagt accttttgac caactcagtt atgtagagtt caagaaagaa
    44341 cgctattatt tgacagatat tcatttccac ttgccttctg aacatatcat caacggaaaa
    44401 caggaagata ttgaatttca ttttgttcat atgaacagca aaaaagagaa tctagttgtg
    44461 ggtgtcatgt accagctaat ggtacaggaa ggttggctgt ataatcaaga aaatggggaa
    44521 agttggaact tagaaaaaca tgagcattgg gtaaatccgg atgtgttctt tccggagcaa
    44581 aaaagccatt tccattatat tggctcattg acaactccgc ctaccagcgg gccaatccaa
    44641 tggtttgtaa tggatcatgt aggaaaactg aaccaagagt ttcttctccc cttcgatggt
    44701 cagtatgctc gaccgaacaa ccgaccaatc cagcccttga atggaagaga aatttattat
    44761 ttcgaggagt ttgagcaatg aatattcagc agatgaaata cgtggtcgct gtagcaaaca
    44821 atggtagttt cagagaagcg gcaaagaaaa tgtttgtctc ccagccaagt ctttcagctg
    44881 gtatcaaaga attagaaacc gagttaggga tcacgctgtt tactcgaacg aatcgagggg
    44941 catatttaac agaagaaggg caagacttct taaatcgtgc cgagaaaata ttggtccagc
    45001 ttgatacatt agaaactcac tatctaacga gtgaaaagag agacaatttt tcgatcgctg
    45061 cccagcatta tgatttctta ggtcctttga ccgcacagct actcgaggaa ttcaaagacg
    45121 agatcaaaca atttcggatc atggagacga caacagcgag agtgatcgaa gaagtcaaag
    45181 agcagcacag tgagatcggc attatttata tgaatgagca taatcaagct ggtatcagaa
    45241 gatatttaca acaaggagag ctaagtggcc gcagcattgg aaaattcaaa acgcatattt
    45301 ttttaaggca aggacatcca ttagctgaac acgcagagat caccaaagaa gaattgttat
    45361 cttatcctca agttcgtttt acacaggatg gaaataattt cccgtatttt tatgaagacc
    45421 ttatcgagat ccctgatcaa gaaacagtga tttatacgag tgatcgaggt actttgatga
    45481 atatcgttct tgaaactgat gcttacgctt caggatctgg tattgtgatt ggagggattc
    45541 gcgagcactt gcggttaatc cctttggcag aaggcgaact aaatgaattt tatatcattt
    45601 attcggcgaa acgtccgctc agtgaaatcg ctcaaagatt tatcaaagag cttactcggt
    45661 tactggacca gcagagctaa gaaaagcaga tgagaacgct gtttattgaa caccgttgat
    45721 caaaaaagag gggttgtgac agaattttgt cacaactcct ctttttgtag caaacaattt
    45781 ataactaatt gctgttgtgg gaaagtacgc aaatatgatt ctagaaacac tcattgatta
    45841 cctgttttgt ttcatagctg taattttttc ttcttctggt gtcacgataa tcgtcttttg
    45901 gttttcataa ataacataac caggcttggc gccatttggt tttcggatat gctttacctg
    45961 tacaagatca accggcactt gggcagagta tcggtatttt gaaaaatagg cagctaactc
    46021 ggcagcttct gtaattgttt catctgaggg cttgtcactt ttaatgatga cgtgcgaacc
    46081 gggtatattt tttgcatgaa gccaatagtc ggttttttta gctgtcttca ttgtcagctg
    46141 gtcattttga agattgtttt taccaacaag aatgagcgtt ccgtcagaag aaaagtattg
    46201 atcaggctga gatggtttct tacgtttttg tttttttaag ttcttctttt tcaggtatcc
    46261 ttctgaagtc aattcttctt taattgcttc gatatccatt gggccggcaa tttccagttg
    46321 agataagaca gattcaagat attggatttc atctttggct tcttggattt gttcgccaat
    46381 cagcttaaca gcgttcttca gcttttggta tcgatggaaa tatttctgtg cattttgatt
    46441 tggtgtcaat gccggatcta gtgctatttt gattggccga tcttcttcat aataattagg
    46501 taaaacaact tcttttgcgc cgcgtggtac ttgtgtcaga aaagtagtaa gtagttcgcc
    46561 atttctacgg taattctcag cgttttcgga ttctttgagt gtttgttcac gttttttcaa
    46621 tttattttta tttcgtttta gttcattttc gatttttcga atcagttctc cgccttgttg
    46681 ttttgcccga tctttctctg ctttttcgtg ataatagcta tctaataatt gactgagtgt
    46741 tggataagct gttacagcag atgcctgttc ggacaatacc tgataagcaa tcggtgtgaa
    46801 aaaatcttta ttttccgttt cgtaaaaagt aggaataggc tgatggctga tggctgagaa
    46861 aaattcgtcc catacaacca tcttttcatt aggacgttcc agtaatcttt tggtcagctc
    46921 ttgagcgata tcgaaaccga ttccctgaaa ctgacgttgg attgcttttg gatctagttc
    46981 agtttgggaa agccgctgaa agattttttc tttctcggag ctaaatgggt tcaagacctc
    47041 ttgttttggc ggagcaacat attctacacc aggaagcaaa gaacgataag tgttttgaga
    47101 gcttccaatg tgtttgatcg catccagtat ttttccagtt tctcgattaa ctaagacgat
    47161 cgtactatga cgtcccatta attcaacgat caatatgata ttttgtagat cacccaactc
    47221 gtttcgttta gcaaagtgga aatggatcac tcgatcattt tcgatttgtt cgatgtcttc
    47281 taaaatagcg ccatccaaat attttcgaag catcatcacg aagttaggcg gattatcagg
    47341 attctgataa tcgatttggg taatctgtac acgagcataa cttgggtgcg cagataataa
    47401 taatcgatgg tttttgccgc gtgaacgaat caccagcacg atttcatttt cataaggctg
    47461 atggattttc gagatccggc cagatagtaa cgtttcacgc aattcgttga ccatcgcgtg
    47521 ggtaaataca ccatcaaagg acatggttca ttcctcattt cttttcataa gtatattcat
    47581 tataacgaaa aaaagaagaa aaaccaattt ggccgaatag taaagcaaaa gtctagaaaa
    47641 atcttggact aattacataa aaaacagtgt ttctatcgtt gaaatccaaa tttttaggt
//
LOCUS       DASHFF010000017        43441 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.017, whole genome shotgun sequence.
ACCESSION   DASHFF010000017 DASHFF010000000
VERSION     DASHFF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.017"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(98..1228)
                     /locus_tag="U2I75_001066"
     CDS             complement(98..1228)
                     /locus_tag="U2I75_001066"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEN1696215.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKE
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="U2I75_001067"
     CDS             complement(1877..3079)
                     /locus_tag="U2I75_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1696216.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="U2I75_001068"
     CDS             complement(3139..3342)
                     /locus_tag="U2I75_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN1696217.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="U2I75_001069"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="U2I75_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="U2I75_001070"
     CDS             4879..5691
                     /locus_tag="U2I75_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1696218.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6714..6866)
                     /locus_tag="U2I75_001071"
     CDS             complement(6714..6866)
                     /locus_tag="U2I75_001071"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1696219.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7057..7935)
                     /locus_tag="U2I75_001072"
     CDS             complement(7057..7935)
                     /locus_tag="U2I75_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1696220.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7974..9092)
                     /locus_tag="U2I75_001073"
     CDS             complement(7974..9092)
                     /locus_tag="U2I75_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN1696221.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9089..11188)
                     /locus_tag="U2I75_001074"
     CDS             complement(9089..11188)
                     /locus_tag="U2I75_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN1696222.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETMNYFAQLFGQLSMSDDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACSGLAEIFEALEQLSLSERYKDIACKLKESICKQMTIDGTF
                     GEQFLEGIGDEEKTTYSVKNYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDPLYQNTMTFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNH
                     QAFVTRLEELICLADLDGSWWWWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIYIQRRKR"
     gene            complement(11239..12060)
                     /locus_tag="U2I75_001075"
     CDS             complement(11239..12060)
                     /locus_tag="U2I75_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1696223.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12057..12866)
                     /locus_tag="U2I75_001076"
     CDS             complement(12057..12866)
                     /locus_tag="U2I75_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1696224.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12887..13381)
                     /locus_tag="U2I75_001077"
     CDS             complement(12887..13381)
                     /locus_tag="U2I75_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1696225.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13393..13881)
                     /locus_tag="U2I75_001078"
     CDS             complement(13393..13881)
                     /locus_tag="U2I75_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1696226.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13958..16654)
                     /locus_tag="U2I75_001079"
     CDS             complement(13958..16654)
                     /locus_tag="U2I75_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1696227.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17082..18145)
                     /locus_tag="U2I75_001080"
                     /pseudo
     CDS             complement(17082..18145)
                     /locus_tag="U2I75_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(18142..18969)
                     /locus_tag="U2I75_001081"
     CDS             complement(18142..18969)
                     /locus_tag="U2I75_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696228.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18970..19776)
                     /locus_tag="U2I75_001082"
     CDS             complement(18970..19776)
                     /locus_tag="U2I75_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696229.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19788..20873)
                     /locus_tag="U2I75_001083"
     CDS             complement(19788..20873)
                     /locus_tag="U2I75_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1696230.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20890..21432)
                     /locus_tag="U2I75_001084"
     CDS             complement(20890..21432)
                     /locus_tag="U2I75_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1696231.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21690..22679
                     /locus_tag="U2I75_001085"
     CDS             21690..22679
                     /locus_tag="U2I75_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1696232.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22676..23413
                     /locus_tag="U2I75_001086"
     CDS             22676..23413
                     /locus_tag="U2I75_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN1696233.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23410..23961
                     /gene="coaC"
                     /locus_tag="U2I75_001087"
     CDS             23410..23961
                     /gene="coaC"
                     /locus_tag="U2I75_001087"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN1696234.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23977..24609
                     /locus_tag="U2I75_001088"
     CDS             23977..24609
                     /locus_tag="U2I75_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1696235.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24694..25341
                     /locus_tag="U2I75_001089"
     CDS             24694..25341
                     /locus_tag="U2I75_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1696236.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25346..26329
                     /locus_tag="U2I75_001090"
     CDS             25346..26329
                     /locus_tag="U2I75_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN1696237.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26732..28246)
                     /locus_tag="U2I75_001091"
     CDS             complement(26732..28246)
                     /locus_tag="U2I75_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN1696238.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28384..29190
                     /locus_tag="U2I75_001092"
     CDS             28384..29190
                     /locus_tag="U2I75_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696239.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29365..29934)
                     /locus_tag="U2I75_001093"
     CDS             complement(29365..29934)
                     /locus_tag="U2I75_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1696240.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30017..32077)
                     /locus_tag="U2I75_001094"
     CDS             complement(30017..32077)
                     /locus_tag="U2I75_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN1696241.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32354..33832)
                     /locus_tag="U2I75_001095"
     CDS             complement(32354..33832)
                     /locus_tag="U2I75_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1696242.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34130..35065)
                     /gene="murB"
                     /locus_tag="U2I75_001096"
     CDS             complement(34130..35065)
                     /gene="murB"
                     /locus_tag="U2I75_001096"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN1696243.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35138..36058)
                     /locus_tag="U2I75_001097"
     CDS             complement(35138..36058)
                     /locus_tag="U2I75_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1696244.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36171..36923
                     /gene="xth"
                     /locus_tag="U2I75_001098"
     CDS             36171..36923
                     /gene="xth"
                     /locus_tag="U2I75_001098"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN1696245.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            37909..38442
                     /locus_tag="U2I75_001099"
     CDS             37909..38442
                     /locus_tag="U2I75_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEN1696246.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(38932..39456)
                     /locus_tag="U2I75_001100"
     CDS             complement(38932..39456)
                     /locus_tag="U2I75_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1696247.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(39449..39922)
                     /gene="tsaE"
                     /locus_tag="U2I75_001101"
     CDS             complement(39449..39922)
                     /gene="tsaE"
                     /locus_tag="U2I75_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN1696248.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(40045..41025)
                     /gene="pta"
                     /locus_tag="U2I75_001102"
     CDS             complement(40045..41025)
                     /gene="pta"
                     /locus_tag="U2I75_001102"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN1696249.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(41178..41858)
                     /locus_tag="U2I75_001103"
     CDS             complement(41178..41858)
                     /locus_tag="U2I75_001103"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1696250.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            41984..42853
                     /locus_tag="U2I75_001104"
     CDS             41984..42853
                     /locus_tag="U2I75_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1696251.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            42879..43394
                     /locus_tag="U2I75_001105"
     CDS             42879..43394
                     /locus_tag="U2I75_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696252.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
BASE COUNT        12948 a         8989 c         7437 g        14067 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caatttcttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cattcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gtgcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatagac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt tcttgctatc tttttataat
     6661 cacaactctc tataatagaa ttacgattta ttttatacgg ctgaaaaaat ctatcaccaa
     6721 aaaaattcaa acattaagcc aaggattatg aagtcaacat ttcgaaaaat gaacatacat
     6781 tggtaagcaa ctataactat taccacagac ataaagtaac acaatatatt actatttaag
     6841 tgtacgccac cagtgaatat cctcaaggta tacagcgcac aacaaaaaga aaaaaggaaa
     6901 cgcagaagca tttcctttca gtgatttaat gaaaacaaca cgatttgact tagagccttt
     6961 ttatttgaat tcattttcgc tttatccaac ttcatttgat catttgatta ctcggttaaa
     7021 tacttttgac atgagtatca atgaaacatt cattaattat ggttgcttat ttcttaccgc
     7081 ttttctggcc aatgctaatc cgccgattat tccggcatta tcacctaatt cgggcgtaat
     7141 aatgtagcta tcaattggtg gtatttcaac atatccattt ataagctcaa caaatttatc
     7201 ctgaactttt ttcttcatgt gttcttgttt cattacacca ccgccaaaaa taattctatc
     7261 tggcgaaagc atcagtgttg tattgtatgc acattgggca aggtaaaagg cttcaatctg
     7321 ccaaaacgaa tcgtcagcca ataaattctg gccttttaca cccaatcgtt tttcaatcgc
     7381 tggtcctgct gccatcccct ctaaacaatc ttggtggaag ggacagtttc ctgaaaaatt
     7441 gtcctttgga tgtcgcttga tcaacatatg ccccatttcc ggatgactga acccttccac
     7501 gaaagtccca ttgaacaagg caccacctcc gatacctgtt cccacagtga agtatgcaac
     7561 acctgctaac cctttgccag aaccagcaac atattcacca tagcaagccg cattgacatc
     7621 tgttgtccat tcaatcggaa tattgaaata ttgctttagc gttcctacaa aattgaaatt
     7681 ttgccaagca agttttggtg ttgatgtgat atgaccgaaa ctcgttgatt tgcattgaat
     7741 atcaattgga ccaaagcagc cgaccccgat gcctgccaat tgttccttaa aaggtttaaa
     7801 aaaatcaatg acctgcttca ttgtttcaac tggagttgtt gtgggaaaac tgactctttc
     7861 caaaatcgtt aattcctcag taccgactgc acagacaaat ttagtccctc cagcttcaat
     7921 tgccccatat atcatcatag gttcctccct tcgaatatgt gaaaaacact tcattaacgc
     7981 ggcaagcgtt ctcgatcagt gaaatgacgt ttagtatttc ctgaagcttc tgttgaatac
     8041 caataggcga cactagcaac atcgtcttgc cgctcaaata agcgaacatc atcgttgccg
     8101 atttgttgta aagtgaccga taaatcttcg gtaaaacaga tggggtcttg aagatgccaa
     8161 cgataaagac catgtcgtgg cattgcgtcg tcaccaaagg catgaacggc attcaatttt
     8221 cccgtttcaa aacgatctct tgtggcatct ctatttgatt ggtaaggata ccctaaaaag
     8281 gctgttgaat agtttttcgc atgtggacgt ccgaactcat cgtactcatg aaaggcccac
     8341 gccccgccaa aataatcttc tgaacctgtt gaggttacgg tcggatactc actatcccca
     8401 tcaatgtaga atttaaactc gccttcgccc caccaataac gttctaggca ggtaagcgca
     8461 aggtaagtac ccacgtaata gccttgaccc tttaatcgat caattaactt ataatcctcc
     8521 tgtaatttgg taatgcgttc ccgatgccaa tttgcgtgaa aatataggct attttcttcc
     8581 aattcgtcca ccaaagcgta atttatcgta taaaaaaatg aggacatatc cttcggatgt
     8641 aaattctcga tcgtaatttt agcggatgtt tggaaaggca tttcgaaata ggaattaaag
     8701 ccccccgtag gattaaccac tataggcaac gagttcacct cgcaacgctc tccaaaccca
     8761 ttacaaaaga aatcccctaa aggactttca acagccgggc tatccgaccc atcccaataa
     8821 attcttaaga ttaaatcacg taaaacaaaa ctgccaaatt ctgttcgatc acgaatcgtc
     8881 atccacatat gacgaattat tcccgttcca gtaatatccg caatcacagt ttcttcacca
     8941 gaagtcagcg gaattgcgcc acgacctttg cggcttggtc ctagctcgct tggcaacatc
     9001 gcagctttac ctttctcgcc tgttggattt tcgggagtga ttgctcgact ctggattttt
     9061 ttaaattggg taatattttt tagtatactc atctttttct cctttgaatg taaatcgttt
     9121 ttcccttttc tacgtgaaga atttttttcg tatcacttat tttcaatggc ttttctgaca
     9181 aatttgaaat actcaactcg gattgcgtac attcaatcga aatgaaggct tgaccgaact
     9241 tcaaattttt ccaagaaaac gttaaattat caacaggatc aatttgcaat actccttttt
     9301 tgaatttcaa tccgaaatat tgactgatca ttatactgac aaaaaggccc gatgcccagc
     9361 cgcatttgcc gcagccaaag tctcgcacaa cttctccttg tttagcctct aaacgatacg
     9421 gccaccacca ccatgaaccg tctaaatctg ccaaacaaat cagctcctct aatcgggtta
     9481 cgaaggcttg atggttgtgc atgtcactca ttaaaacagt gataaagcca ggaaaagtgg
     9541 cgccggattc taatccccaa gtgatgcctt tgatttcctc gctataagtt ggattgaaag
     9601 aactagcaga aaaggtcatg gtgttctgat ataaaggatc tttattatct aaaaattgat
     9661 aaaaaggcat caacgtagtg tctgattctt ctccatcatg catcaataaa ttgattttcc
     9721 catccacaat cacatcactt aaaaagatta acccttgctc aagaatcggt ttttgatagt
     9781 tcttcactga ataggttgtt ttctcctcat caccgatccc ctccaaaaat tgctccccaa
     9841 aagtaccatc tatggtcatt tgtttacaaa ttgattcctt tagtttacag gcgatgtctt
     9901 tgtatcgctc gctaagactc agttgttcta gggcttcaaa aatttctgca agtccactac
     9961 aggccttcca aagacaaaga ttgctgccgg tatgatactt tccaagtgca aaggcgtctg
    10021 ataaccaaac agatggaaaa agcattggtt cccctgaccg acgttgagct aatatgtcct
    10081 caataattct tgaagcattc agtagaacct gcggacaccg ctcaaagaat tttgtatccc
    10141 cagtatgttc aaaataaaga ctagccagta acgcactgga taacgaatta ctcagagaat
    10201 gattgatacc tcctgtaaat tttgaaccag gaaacttaac gccatattga tcaaaccata
    10261 gaaccgtttt ttgacataac tccggttcaa acatggtgaa tggcaatgat gcgtaataca
    10321 tatccttatt ccaaattcga ttagtagcag gatagctgcc ccaattgctg ccgacgacct
    10381 gtccctgtcg attcataccg aaactagaaa agctttgata aagtgcacga cgatagagat
    10441 ggacaactct atcgtctgac attgacagct gcccaaatag ctgagcaaaa tagttcattg
    10501 tttccttcag ccatttttcg atatcagatt cctgaaaagt cattacttct tcaaaagcat
    10561 ttgggtccac aaaagcaatg gcgaagcttg ccacttcctg ttgtgcgatc gtgtccttat
    10621 ttcctttttg agaaatcaac acattttgct gatccgaata cttctcttgg tacaaaggca
    10681 catctttcac tgaaacttga aggctttcgt gtgtttcatt cacaatgtaa acctgttgaa
    10741 caagcatact taaacgctta ttagccagta ttggagcaaa ggatactaca atgaccttaa
    10801 acgtcgaata atgatgaata tatctaggca aacagtcttg aatcacatcg acttcaatac
    10861 gatgatccga ttctgacaaa tggtagacaa tcccgtttac ttcaaccttt aaggataacg
    10921 aattggtttg agaaagctgt ttattgaccc aaacacctgg tttttcctca gttagtaaat
    10981 tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac atcaaggttt cctaatgtct
    11041 tttgattact tacatcaaag gtcaatccct ttacatcagg aatattccga tagtagctgt
    11101 attcgtttga agtagtcaca tttccgtcgt ttaactcagc attaaataat gggtgtttgg
    11161 gtgaataaat tacttcatta atagtcatct tttccctcct tgctaaaaaa actgcagcct
    11221 agtagctgca gcttcctact atgcgattcc aagtaatgac aataaaattc ccaagacgac
    11281 cattccaaaa ataattttga tgacgccgac ttttttatct agtaatttga aacagcccaa
    11341 tgttaccaat aacggtacaa ttcctttaaa aatactatcg aggatttctt ggacattcat
    11401 tagttcttcg cccttcattg aaaagctcaa aacggtattg aatttgacca tactgatcgt
    11461 cattgcgcct accatgatca gacccaagat actagcagct tttgtcagaa tttcaattaa
    11521 cccgttcgca tacagctttt caatatattt agagcctaat gaatagccta aaacgccgcc
    11581 atagtatcgc atcaaataat taggtatatt gaacataact aaaaagataa ttggtgccaa
    11641 aatatttccg gcattggcta agttgatcgc tagtcccgcg gtaacgacac gccaaactcc
    11701 ccagaacaat gaatcgccaa ttccagccaa tggtcccatc aaactcgttt tgatcgcgtt
    11761 gatactttcc acgtcaaaac cagctttttc agaattttcc ttctccatcg atgccgcaat
    11821 ccccatgaca aaggacgcgc aaggaatggt cgtcgaaaaa tactgtgtat gacgttccag
    11881 cgccgcagct cgatcttctt cacttttgta gaaatgatta atgaagggca tcatagaata
    11941 ggcgaaacca ccgccgccca tcgtcaccgg attcaccgaa gcatacatgg tcatcgagcg
    12001 ccaaaacatc ttccgaacca attttttctc atctttagaa attccatcag ctaatttcat
    12061 tgaaagaaat cctcctcttc ttggtcaagc gttgcattta cagcagcatc gctttgaact
    12121 gcatgaacag atatattttt tagttgtaga tctctttggg ctataacaat tgcaataatc
    12181 gttccaactg cggcaattgc aatcaatggt aactcgagat acgccataac aacggcccca
    12241 aagaaaaacc acacagcaat atttttactc caaagcatcc gtaacagcat cgccatccca
    12301 actgccggca gcagatttcc agcgactgtt aatccgttca tgatgacctc tggaatatta
    12361 tccaagacgc ttttgacaac atctgctcca aataaaacgg caaagaatgg aataagagca
    12421 taaagcgcat agtggacaaa ccacataacg aagtgcagat tgatgattcc tttttgatca
    12481 ccagattttg caaatttgaa aaatttagca gccatcggtc cacatacaaa agaaaaaatc
    12541 atattcatta actgtaatcc taatactgct agtggaaaga caatgggaat aatcgcatcc
    12601 acgtttcctc ccattttgat tgcgaacgct gtagccaacc cagcagccaa agaaggctcc
    12661 gccgtactag agattccgat attgacgaca cccataaaaa tggcttctag tgacgccccg
    12721 acaatcaacc ccgtctgcaa atcccccatc aacgctcctg ctaacggagc aacaacaatc
    12781 ggacgactca gcatggacat cccgaacatt tcatgtccag caactccaat aaaaacggcc
    12841 aaagcgacta caaatgcatc atacataatt tttctccttc ctttttctaa aaatcactca
    12901 aaatagagat agctgaacgt ttttccatcg ccggtgtagg ctgaaaaacg gtatctggat
    12961 aaagttcaac taattctttt aagtcttgaa tttctgacgt cgttaacatc acttcctttg
    13021 ataaggtttt cttatcttcc gatggcggtg tcattttccc agcattcccc acgttcatta
    13081 attcgatttc cgttgggaaa gctttcgcaa cttttaccgc atctttgaca ttagggacta
    13141 aaaccatcaa gcgcatgtcc ttcgcccgcg gatcttttaa caaatgaatc gcgtcatcaa
    13201 ctgttttaat aatgactttg acattcgatg gtgcagccat cttcaatgtc atcttttgaa
    13261 tatcgttggt agccgcctca tcgcttgcta ctactaaccc ggttaaattt agactctttg
    13321 tccaagtcat cgctacttgt ccatgaatta atcgttcatc aattcgcact gctttaatca
    13381 tttttcttcc tcctaaaaaa aatcttcctc ttcatttact acatgtacct catcaagaat
    13441 ggtcatcttt aattcctgtt ggcatttatt taccagttcc tgaatatcct cctttgttaa
    13501 ttccttggtt gccaatacta tttcgagcag aattggcaga ttaaagccag acaaaatagt
    13561 tacagcaatc tctttctcca aaaaggtttt ggtaatcttc tgattgacac ttccgccaaa
    13621 taaatcggta aaaacaaaaa tctgatcctg ctcgttcacc tggtttacaa atacattcaa
    13681 ctcctgttca aaatcacact catccacata agcattaatc accgtaatct cttgaacctt
    13741 ccccgtcagt aattgaatac tgctaagaaa gcctgaagct aagtgaccgt gagtcgctat
    13801 tagaattttc ttcatatggt taattccttt ctttctgttc acttttatat aagcaattcc
    13861 tgtgccaact tttaaaaaca ttggtttaat cacgttaatt cacattaaaa aaagtgtcta
    13921 acctaaaaaa aacagattag acactttttt agacacttta tttttgaata tacggtccta
    13981 ataattcata gagcagcgaa agctcataag cattgacttt aaaattatat ttcatctcca
    14041 ttgattgaaa aatacttcta gaaacataat aaaattcatc ttcatctggt ttcaaatcat
    14101 aattcggttc ttcagatttt tctgtccgag ccaagaataa acgttccatc atcaatgccg
    14161 tatgcatata gagatttagt tttacacggc catttaaagt gaaatgatag taattttcat
    14221 atttagaaat gactgtttcc acttcactca tcacgatcgt tgggttcaga aagttcaaac
    14281 ggtccgcaat tccttccaac gaaaagaatt ttactagctc ctgaatcaag cggttaaatt
    14341 ctgccggctt catttcagga ctcaaaatat gctccaaatt ggctgctcca tcaccgtcta
    14401 agatgtcata aacattaata ttaggaattg aaaaggaacg tggaagatcc gaagtcgtga
    14461 tgaccaattt tgtcttgtca aaataagttt catcattttg gtcgattaat tcacgtagtt
    14521 ctctgtagtc cttcgtgaaa acttcgatgg attcatccga taaatgatga cgaaaaacat
    14581 ctttcaattt gtccgaaatc cccaaacccg acatacagga aatgataatg ttgctacttt
    14641 gagaaaagcc ttcaaagtat tgaacattga ttgaatactt gtctgctgca tcctccgcaa
    14701 tctgtttaaa tggcgcattc atcgtcatct tcaagccaat atcaagtgca atggaagtcg
    14761 tcaaattatt cagaagaagt aactcccctt ttaaatcgtt cttgatctgt gtgtaaattt
    14821 gatttaaaga gcccatatct acgatcagca ctagactatc tgtattcact tgccgtttca
    14881 cgaaattcct tgtcgattcg acaatctctg acacattact ctctactggc atatccaacg
    14941 cttcaaaaac ataattgccg cataattgat tcactactgc ttgaatgctg ctagccgtac
    15001 tatctccgtg agcaagcaac aggcccttca gctcgatgga ttcatctaca taatcagata
    15061 aagtcacgac aataagcagt cttagaaagt tctcgtcctt ttctgagagc ggtggtaaat
    15121 gctccaagaa ctgattacat aagtaagtcg tccgaggtag atttgcagct aatgtacgat
    15181 ttatcccggt taagtctgtc ttatagcttg gggaaatgac ataggatctg ctaataattt
    15241 ctgcaacagg tccactcgtc gtcaatccgt acgtttttcc taaaacggat acccagacct
    15301 ttctaaacat ctgaatcatc aattttctac tcattgataa aaaagaatcc ttatcaatgt
    15361 gatgcaccaa atgctgtact tcattcttta tcgaagaaaa attttcattg aaattacctt
    15421 catttatcag ctgagtaatt ttttccaaaa tcgaatcata atcaaacggt ttgattcgat
    15481 ccgtttttat cttttgattt ttctttatca ataaactagc aatcggcaag ctttctactt
    15541 cggattccag catatttaac tgaatcaaga tctcattttt cccaatcgtt aatggttctt
    15601 cttgtgttcg actatacaca tccgcacaac tgattttaat gatattcttc agcttaccaa
    15661 tattcccact aaagggatga gataacatta aagacaaggc ttctcttgaa acaataatat
    15721 ctttttgtaa aacatccgct tcattttgaa aaaataattg aatcagctct aatcgttctc
    15781 gaatggggcg atcttcaaaa gttggcaaca taacactaat ttgaatacgc cgatcaaagg
    15841 tgtctaataa ataattttca cctttttccg tcgtggcgaa aataaaacga accttagcag
    15901 aatgccaatt tacattttca ccgactggac gatagtttcc agtatcaata aatagaaaga
    15961 gcttttcttg attttctttc gataggcgat ggacctcatc gagaaaaaga tagccgttgt
    16021 tggccgttgc caataaacca tcggtatcct tttcagcccc cgtaaaggcc cccttcttgt
    16081 atccaaacag tgttgccgaa agcagttcgg gattgtccgc ataatcggca cagtttaaga
    16141 tagcaaatgg tgcatctttt tcaataattt gctcatggat agcgtattca aaaatttttg
    16201 tggctaaaaa actttttcca acacccgtgg ctccatttat taatacactg agtccgttag
    16261 gcggatattt gacagcggct atacattttt gaatgacctc tctcagactt ccgtcatgac
    16321 caactacaga atgaaaggct tcgtttattt gctccgtatt atctacaacc gcatattcat
    16381 ctaaaggctt ccacaaaaca ggctttcccg gtaatttctc gaccttttta tcttccacta
    16441 atctcgtcaa ataatggctg gcattctgac gagagatacc tagattcttc gaaattttct
    16501 cggcagtgac agaagacata ttccctgtta gtcgtatcag ctcatcaaaa actcgatctt
    16561 tagcgctctt tttcattttt tcctccagtt taatgtgtct aaaagcataa taaactatcg
    16621 gctgcaatat gtctattcta tatttgtatt ccaattttta ctttgaaaca caaatagtcg
    16681 aatatttgcc tcagatatct gacaaaataa tttatctacc aatttagttt ttatctaata
    16741 tgatttttat gttttttcat agctacaaat attgaactgg attcatatat tagttttatt
    16801 aatagattac agaagggtta agtatatttg catagtttcc aattacttta tccttctgta
    16861 actttttcaa tcttagattt actagcccgt ctcgggccct attttacaat ggttttgtcc
    16921 acttttctct ggtagaaaaa cgtggtagaa aaaataaaat aacacaaaaa aagcggcgtt
    16981 tcatccgctt ttggtagaag attggaaaac tcagatagaa aaaggacgat ttttagctct
    17041 ttagaacgtt gataagtccc cattcttact gagttttgcc attatctcct atacatcttg
    17101 aattctaaga ataggtcatt atagatgcct aggtatttcg atcctagatc tttgtagact
    17161 tcaagatggg agatcgtgtc atcagatgga tagaattgtt cgtccgacga aatgtctttt
    17221 ggcaataacg cttttgcttc ttcatttggt gtagaataac caatatactc tgcattttgt
    17281 gccgcatttt ccggttctag cataaagttg atgaaatcat aggcaccttc tttatttttc
    17341 acggtttttg gcataacgat attatcaaac caaagattgg atccttctgg tgggatcaca
    17401 taatgtaaat gttcattttc ccacatcata tcggcagctt cgccagaaaa tgtcactgcc
    17461 acggaggctt cttcattgat catatacatt ttgatctcat ctgccacgat tgctttgaca
    17521 tttggtgtta aatgattcaa tttatttgct gcttctgtca gctgtgccat attcttacta
    17581 ttcctaaact attcaaagaa agccccataa cttctcgtgc accgtcaatc agcatcaaac
    17641 tgtcttttaa ttccggcttc cacaaatcat cccaatgctt aatttgattt gcatcaaaga
    17701 atttatcgtt atatacgatc cctaaagttc cccaaaaata tggaatcgaa tactgattat
    17761 ctggatcaaa atccaagtct aaaaatcgtg gatcaatatt atttaatcct ttgatcttcg
    17821 aatgatctaa tggaagtact agtttttctt tgatcatttt ttggatcata tattcacttg
    17881 gtatagcgat gtcgtagttt gtcccacctt gctggatttt tgtgaacatc gcttcattag
    17941 aatcaaatgt ttcataattc actttgtatc catattcttt ttcaaatttc gtgatcaaac
    18001 taggatcgat ataatctccc cagttataga tggtaagtac ctttgctcca ctcatgccac
    18061 ttgttttttc aagttgacga acaccaagca gcagcaccgc aataatgatg agaataccta
    18121 aaaacagaga ttgtaatttt ttcatcgcag ccgtgccacc ccctcgcgta ctcttctgtt
    18181 tttcttcagc cgttttggac ggttttcttt actaataaag tagtatccaa tgaccaatat
    18241 catggagaac aagaaaacta acgcacttag cgcattgatt tctaaactga ttccttgtct
    18301 tgcacgagaa tagatctcta ctgagagcgt cgtaaagcca tttcctgtga cgaaaaatgt
    18361 aaccgcaaaa tcatcaagag aataggtaaa tgccataaaa tatccagcta tgattcctgg
    18421 agaaagataa ggcaaaatga tatttttgac tacttggaaa ttattagcac caaggtctct
    18481 agccgcatcg accattgaat cgttcatttc ctgcaatttt ggcaagacca tcaagacgac
    18541 gattggaatg ctgaaagcaa tatgggaaag caatacactc tgaaaaccta atccgaatcc
    18601 tagtagtgta aagaaaatca agaaactagc accgataata acatcaggag agactaataa
    18661 gatgttattc atgctcaaca acgctgtccg tgcacgtcgt tttttcgtgt aatagattcc
    18721 catagcccca aatgtcccga tgatcgtggc gatcaatgca gataagaaag ccagcatgaa
    18781 cgtattcaat acgataatga tcaaacgtgt atcttcaaaa acagcttcat aattttccca
    18841 agtaaatcct gtaaagctgt tcatcgttcc tccttcattg aaggaataaa aaatcagata
    18901 gaagatcggt gcatataaga ataaaaagac gatccctaaa taaagatacg accatttaaa
    18961 ttttttcatt tatgagcgac ctcctttttt cttctcgcct gtcaggagca tcacgatgaa
    19021 catagcaatg attaaaatca caccgattgt agaacccatc ccccagtttt gtgtaaccaa
    19081 gaaatgctgt tcgatagccg ttcctaaagt gatcacccga tttccgccaa tcagacgagt
    19141 cagcatgaat aaagaaagag aaggaataaa tactgcttgg acaccacttt tcacaccatt
    19201 taatgataac ggaaaaatca ctcgtcggaa ggtctcaaag ctattcgcgc ctaaatctct
    19261 actagcactg atcaaagaag gattcatctc ttccaatgca ttgaaaattg gcataatcat
    19321 aaatggaatc tcgatatacg ttgccacgaa cagaaaactg aagtctgtga ataatatctg
    19381 tttcggcccg atcccgataa aatcaaagaa atgattcacg ctgccgtgca cactgaaaat
    19441 cccaataaat gcgtaagctt taagcagcag attcacccat gtaggcaaaa taatcagcat
    19501 cagccataat tgcttgtgct tcagttttgt taaaaaataa gcagtcgggt aactgatgat
    19561 cagcgtgaaa aacgtgatca aaaatgcata ccatacagaa ttgatcgtca tagaaagcga
    19621 tgtccccgac gtaaaataag atagataatt attcagcgtg aattgcccat tcatatcaaa
    19681 aaaagattga taaatgatca ttaatacagg cgcgatcacg aataaaccga gccacaaaat
    19741 ataagggacg gaatagatcc tgcgcattgt tttcattctt gtccccccta ttcttcgtag
    19801 ctgtcaagac gtgcatcgaa ttcttcttca gactcgttga aacgcatgac atggatatct
    19861 tctggctcaa aggccaatcc tactttgctt ccttctttag cttttttcgt tgaatggacc
    19921 atccattcat tttgctgttc atcataacag atgatttcat aatggacacc acgaaacagc
    19981 tgagtatcga ccgttacgac tagttttcct ttatctggtg tcgtgatcgt caaatcctct
    20041 gggcgtaaaa caacttcaac tggttcattt agacgcatcc cgccatcgac acattcaaat
    20101 tgcttaccaa caaactcaac tagattatct tcgatcatta caccgtccac gatattgctc
    20161 tccccaacaa aatcagcaac gaaatgattg attggctcat cataaatatc tacgggtgta
    20221 ccactttgga cgattttccc tttattcatg acaaaaatct catcactcat tgccaacgct
    20281 tcttcttggt catgagtaac aaaaataaac gtgattccta atcgttgttg cagatcccgc
    20341 agttcatact gcatatctgt acgtagtttc aaatcaagcg ccgataacgg ttcatctaat
    20401 agcaatactt tcggttcatt tacaattgct cgtgcaatcg ctacacgctg tctttgacca
    20461 ccagacattt cactaatctc ccgtttttcg tatccaggaa gctggaccat acgcaaagca
    20521 tcttttacct tcttttcgat atctgatttg gacattttct tgatcttcaa tccaaaagca
    20581 acgttatcaa aaacattcat atgaggaaaa agtgcataat cttggaagac tgtatttact
    20641 tgtcgcttat tagctggcaa atcattcaac cgtttccctt caaaatagat gtccccttct
    20701 gtagcttcgg tgaatccagc tataatacgt aggatcgttg ttttaccaca gccggaagga
    20761 ccaagcagtg tataaaattt tccttgttcg atctcaaaac ttactttttt taatactgtt
    20821 tcatcatcat agcgtttcac tacttgatca aatgaaatga ttggtttccc cacgttttta
    20881 ccccctatat tacaaatatg attgcgttgc aacaatcaat acttttgttc gttttttgag
    20941 atgattcctt aattgatgtt gttttgtcgc ttgataatag atcgactgtc ctgcttttgc
    21001 cacatagacc gtctctccta agcttaactc taattcccct tctaatacaa aaataaacgt
    21061 ttcagataat gaaggttcaa acgtcttata ttctcctgca ccttcaaaag tcagcaagac
    21121 aggctccatt tctttttcat tcgaatcagc cacgagccat tccaattcat acccgttttc
    21181 ttcatcgaaa taaaccgtat gatcttcttt tgaataaacc acttgcagat tcgctgcttc
    21241 ttgatgaaaa aattcttctg gagtcacacc taagacttca agaatagaaa agaatgtttc
    21301 cattgatggt gaacttaaat ctcgttccaa ctgggaaata tacccttttg acaaatctgt
    21361 gcgttcgcct agttcttctt gtgtcaaatt cttttggatg cgcaaatttc ttaacttctc
    21421 accaatttcc ataatttcct ccgaatcgaa gttttctaat agtaaacttc ctgtttacta
    21481 ccactcgtat tagtatacga aatttattga aaaaggcaac cctttgtaaa attttttttg
    21541 ctgaatattt ctttatcttt tggaataaat tctagttgtt tgattcttta tttttcacct
    21601 aatttttgtc taaatttacg gaagaacaag aataaaagag taggctttat ggtatgataa
    21661 aagaaaattt taaaaggagt gtctcacgaa tgaacgcttt tcgatctttt caggtaataa
    21721 aggaacctgt tttccataca gcaatcagtg atctgccatt tactgagttg gctgaaaatg
    21781 aagtcttggt aaaaattgcc tattctgatg tcaattataa agatgcattg gcaatgagcg
    21841 agtctggtca ggtgatacga acatatccga tgactccagg gattgattta tccggaactg
    21901 tcgtccaaag taacaatcca cgtttttcaa aagaagatct tgtacttgct actggttttg
    21961 ggcttggcgt cacccatcca ggcggttata gccaatatca aaaagtacca ggagactggt
    22021 tagttcctct accaaaaaat atgacgctgc gtcaagcgat gatccttggt acagcagggt
    22081 ttactgctat gttgtgtgtg aacgctttaa tccgtcaagg tatgacctcc gataaaaaag
    22141 ttgtcgttac tggtgcttct ggcggtgtag gcagtacagc gatagccctg ttacataaac
    22201 taggattcca gtcaatcatt gccttttcca gaaaagaaga gtctgtcaca tggctaaaaa
    22261 gtctaggtgc tttgcaagtc gtacgacctg atgaatttct gccagagaca acaaaagctt
    22321 taggaaaaca gcaaatcgac tatgtcatcg acacagtagg tggcgagcag ctaagcagtc
    22381 ttcttccatt gatctcttat aacggtgcag tagctttatg tggcaatgct gggggaatca
    22441 aactaaacgc gactgtcttg ccttttattc tgcgaaacat ccagttgatt ggaattgatt
    22501 ctgtcaatgt accaatagat caacgtcttt ctttatggca acagatggcg gatttgcaga
    22561 ttgcagatga actggtcgtc caagagatta ctctggatca attgcctgaa acagcatcta
    22621 aactacttgc tggaacacac caaggaagaa cactagtaaa tgtaggtgac cataaatgaa
    22681 gattctaatc acagctggag gaacgactga aaaaatcgat caagtacgtg ctattacaaa
    22741 tcattcgaca ggacgattag ggcaagcatt agctgattat ttagctgcca atccggatac
    22801 cacaatcgat tacgtgacta cgaaacaagc actgaaaccg aaaagacact ccaatataac
    22861 gatttatacg atcgaatctg cactggactt atttttacaa ttagaagaac taacgaaaaa
    22921 agaacactac gatgcgatta tccacagtat ggcagtcagt gattttactc ctgcttttag
    22981 tttttcagaa gaacagctgg caaaaaacct acccacttct tcaacgcaag aagaactgga
    23041 caattggttt gcggaaaatg aacagacaga taccactgtt tcaaagatct cttctaacac
    23101 cgagcatctt gtgctcgttt tgaagaaaac accaaaaatc atttcttatc tacgtgaatg
    23161 gcagccaaaa gcaaaaatca ttggtttcaa actattagta gatgtaccaa aggaatctct
    23221 tctggcagtc gcaaaaaata gtctgataaa taacaaaact gattttattt tcgcaaatga
    23281 tttgacggaa atccatggcg agacacacca cggctatctc ttatctaaag atggaacagt
    23341 ggaagaagct caaagtaaat ctgagattgc tgcattgatt actgagaaaa ttagactgga
    23401 ggaatcaaaa tgaaaaagat cttactaggc gttactggaa gtatttctgc ttacaaaagc
    23461 gctgatctta caagtcaatt cgtcaaatta ggtgcccagg tagatgtgat tatgtctaaa
    23521 agcagcacac attttatcac gccattaacc ttgcaatctc tgtcgaagcg cgcagtccat
    23581 acagacgtca tgcaagaatc aaatccgcag gtaatcaatc atatcgaact agcaaaacaa
    23641 gctgacttgt ttctcatcgc gcctgctaca gcaaacatta tcggcaagct agcaaacggc
    23701 attgcagacg atcttctttc aacagtcgca atggcgcttc cagtgggaac accaaaattg
    23761 attgcccctg ccatgaacac gaatatgtat caaaacccga tcactcagcg taatctggaa
    23821 acattgagat ctgtcggcta tcaggaaatt gaaccaagag aatctctttt agcttgtgga
    23881 gattttggaa aaggtgcctt agctgatatt cagacgatca tcgaacaggt catgctgact
    23941 cttaatgaaa gaaatgctta gaaaaaagga gtacttatga aaaatacaaa aaactttaca
    24001 ttaactgcga tgtttttagc tattttgatt ttattagctg ttacgccttt aggttttatc
    24061 cctatcggtc caatcaatgc aacaaccatg catattcctg taatcattgc ttcgatcgtt
    24121 ttaggaccaa gactaggtgc ttttcttggc ggtacatttg gactgatcag catgatccgt
    24181 agcacagtta tccaaacacc tttgtctttt gtcttttctc cgtttatccc agtcatcgga
    24241 acagaacatg gaagctggaa agccttgctg atcgcgttta ttcctcgcat cttgattggg
    24301 gtggttcctt atttcgttta taaaggtctg cgcaaactaa tgaaacaaaa agcagattcc
    24361 gtttctttat tcttagctgg actttccggt tcattgacca atacgatttt agtgatgaat
    24421 atgatctact ttttattcca gcaagattat gccaaagtca tcggaacaaa tttaaatgct
    24481 gtttattcag ctattctagc ggttattttt acaagtggta tacctgaagg aatcgtcgca
    24541 ggtcttgcga ctgcagcagt atgcaatgtc ttgttaagat acttcaagca cccttctctt
    24601 actaactgat agtcatcgta gccgatctct gatttttttc tcttttggtg ttactattga
    24661 atggaaagat ttgacgtcga aaggatgatc caaatgaaaa tttttgttgt aggtgcaaac
    24721 ggacaaattg gccgacacct gataaaagat ttagctccaa gttcccatga aatttttgca
    24781 ggggtgagag atgttgcttc ccaaacatta gtaaaaaaag agaatgtttc gtatgtctct
    24841 tttgatttaa cttggtccgt ggaaaaaatg accgaagcat tcaaaggcat cgatgtattg
    24901 atttttgctg ccggctctca aggaaagaat cttctgcaag tagacttgga tggcgcaatc
    24961 aaaacagtga tcgcagctga aaatgcgcat gtctctcgct atctgatggt cagtgctgtt
    25021 tatgcagatg aaccagcaaa atggcctgag tccatgacag attattatat tactaaacat
    25081 tatgcagatg aatggctgaa acgaactaac ttagattttg tcattcttca accagtcaca
    25141 ttgactaacg atgaagaagt tacttccatt caattaacta agcctaatga gaaggcatca
    25201 aaaacgatca ctcgctctac agttgctgct gtacttgccg ctctagtgga agaaacggat
    25261 atttctcgta cgactctagt actttctgaa ggtagcaaag agttgaatac agcattccaa
    25321 gaatgggcaa aggaggaata gatccatgcg tgccatttca atcaagcatc ctggttctgt
    25381 agatgtatta gaacaaattg aagtgcctgt tcctaaacca aaacaaggcg aattactgat
    25441 caaagtccat acagcagctg tcaatcgaac agatattatg aaaagagaat cagtctcttt
    25501 gaaacctccc taccctattt tgggagtcga agtagctgga gaggtagttg agaataaaag
    25561 tgatatctcc acttttactc ctgggacaaa agtatacgga ttagtcaatc taggtggtta
    25621 tgccgaatat gctgtgatgc ctgccgatcg tgcaatcctt ctccctgata cgctagacta
    25681 tgtttctgct gctggaatct cagaagtttt tttgactgct tatcaaacac tttactggct
    25741 tggaaaatta caagaaggcg aaactgtctt gatccatgca ggagcaagtg gtgtgggaac
    25801 agcagcgatc caattagcta gacagctttc tcacgcgaaa atcatcgtta ctgcaggatc
    25861 agaagaaaaa ctcgcctttt gtaaagaact tggtgcagat gaattgatca actataaaaa
    25921 acaagacttt caagaagagg tacgaaaaat cactgaaggt cggggagtcg atgttatttt
    25981 ggatttcatc ggcgcttctt actgggaaaa gaatttagct tctatagccg ttgatggacg
    26041 ttgggtcttg attggtatgc taggtgggac catcgtgcct gagatagacc ttggtgtatt
    26101 gattgggaaa cgtgtccaac tgcttggtac attattgaca cctagaagtg atcagtataa
    26161 agcagagctc gcacaaactt ttatgcaaaa agctgggcct tttctagaaa atggcaagat
    26221 tcggccaatc attgaccgta cattccaatt cagcgcagtc aaacaagcgc atgaatatat
    26281 ggaagcaaac aaaaacatcg gaaaaatcat tttgaagata actgaataaa atcatgctga
    26341 tcaaaaaaga ggctgggaca gaagcgttta actccaagaa ataagaagaa attcacgaaa
    26401 attgcttttc aaatttttgt gaatttcagc ttatttccga aggaatttct tctgcttccg
    26461 ccgtttatac gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt
    26521 tttccgaata aacggtgttc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa
    26581 aacataagaa gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca
    26641 caacctcttc actaaacagt gttcaatcag ctgaccctcg gtattagctc aaaacgctgg
    26701 tttcacaacc tctttttatt tacgttctat ttcattcatt ttccgaagca aatcgagctg
    26761 cttgtatgcc cgcttgtcgc ccaaaaataa tgatctccgc aactgaattt ccaccgatcc
    26821 ggttgtttcc atgtaagcca cctgtgactt caccagcagc atataacccc ctaattggct
    26881 ggttttcttt atctaatact tcagtgttcg tattgatttt cacaccgccc atcgtgtaat
    26941 ggattcctgg tgcgattttg atcgcataat aattcggaga cgataagtca tgttccatgg
    27001 ctgtggcacg gttgaattga gcatcttttt tctccgcaac atccgtattc catccatcaa
    27061 ctgtttttgc tagtgtttct tttggtaaat cgattttttc tgctaattct tccattgtct
    27121 ttccagttgt gacatatcct ttttggtcat aaaattcaat agctgtggca cgatcacgca
    27181 cgccttggtc aaaaatcaaa taagctgatt tttcaggcaa tgctgtaatt gctgcagata
    27241 ctttatccct tgtatccatt tcattgacaa atcttttccc ctcttgatcg accaagattg
    27301 ctccttctcc gcgaacaacc tcaccaatca agaccccctc atcctgctgc acagttggat
    27361 gaatttgtat tttatccata tcaacggttt gtccacctaa tttttcaatc atggtgatac
    27421 catctccagt tgatccttct tgatttgtcg taatataatc agctaaatcg ggacgatatt
    27481 tttcaataaa ttctttgctg gcaccaaaac cgcctgtggt aactacgatt gcttttccgg
    27541 tgacctcttt tggttcttct ccttgaacat gtacagtcac ttcattgacc gtttccccat
    27601 ttttcttaat gtctgtcaca tctgcgttga caaaaatagg gatttcccgc tcatggacgt
    27661 tattcaataa cccctccact aagtatccac caatagccga gccatcagac ggacgatgtg
    27721 tcctcttttc actcatccct ccagtgatcg ttaagttgtc cagtttgata ccgttttgat
    27781 ctagccaatc gaccgcatca gcagagtgat cgacaaagta tcgaagcatt tctttatcat
    27841 tcgttcctcc accacctttt agcgtttctt catagaaggc atcattcgag tctttgattc
    27901 cttcttcttt ttgaaatttt gtttctgaag cattcatacc gcttgatgat ttagatgtgt
    27961 ttcctccggc aacaggcatc ttttccaaaa ttactggatt caacccagca tcttttgctt
    28021 cgatagctgc tgccatccct gcaccaccag ctccaataat cacgacatca taagattctt
    28081 tcatttcttt tggatctgta tatacttgtt cagaagcgcc tgatgatact tcggcagatt
    28141 ccttcgtact agaacttgtc tgtgctgtat ttttctgatc tgcgccgcat gcgctcatag
    28201 caagaagtgc aacggctgtt aataaaccag caactaattt tttcatttgt atcccccttt
    28261 tctccttata ctatattaag tgattttttt cacaatatca actctgtttt tctttaagtc
    28321 atatagcttt ggtaaacaca cgcttcttag tataattaag gatatacatg aaggagttat
    28381 tttatgaatc atttgaaaac cttttttacg aacagtacaa aaactgcagg tagaatcaca
    28441 aacggaatct atattttatt acgtatcatc gtcgttatta tgctagtgga tcaggtctgg
    28501 aaccaacact ggggaaatac aatgctttta ttactgacat taattttatt tgaaatccct
    28561 tattttctgg aaaagatatt aaagatacag attccaaata tgctagaaat cattttgtta
    28621 tcttttattt ttagttcaac agttttagga gagttaagtg atttttatgg ctatttttca
    28681 atgtgggata cttttctaca tgcattcagc ggatttttag ctgggggtgt cggcttttcc
    28741 cttgtttatt tgctcaataa aaatacaaaa accatgaact tgactccttt attactagca
    28801 gttgtttcct tctgtttttc gatgacagtt ggtgttatgt gggagtttgt ggaattttca
    28861 gcagataaat tactgcaatt cgatatgcaa aaagatacga tcatccagca aatcaatagt
    28921 gtggagttaa gtaaagacgg aaacactgtc tatcatgttc cagatatcac tcggacaatc
    28981 attgaaagtc aaaatgcaga tggtaaaaca gataaaacaa tcatccaaaa tggttactta
    29041 gacgtgggaa tcatggatac tatgaaggat ttatttgtta attttattgg agcagttgtt
    29101 ttcagtgtat ttggttacct ctatagtcga aacgattacc aaaaatttac gttcatcaag
    29161 aattttattc ctaaaaaaat caaacgataa aacatgctaa aaaacaagag aaggggcagt
    29221 gacaatactt ttgtcactgc cccttctttt ttgaatgtat ggcatgccat tagttatccg
    29281 atagtattaa ctcaaaaaag taagatatga ggaatgacta tttcgttttt tatcccaata
    29341 ctgaggactt atttcaccat ttcgttattt atctaattta cttaaaacct gcataacttt
    29401 ttcataatct ggattaggtg tgccgtctag attttcagca ataaaagtaa aacccatttt
    29461 tttataaaga gcaatcgcct gatggctcca tgtctgtgtg tgaagataga ttggtccgtc
    29521 cgtatggagg gtaggaaatt cttgcaagac tctggcagtc aatgcttttg cgagcccttt
    29581 tccttggtaa tctggtttca ctgctaacca atgaaaaacc ggatacactt gctctcttga
    29641 tttttttcgc caagcggtac aggttgctac tttttctcct ttttttgtct ctacaaaaaa
    29701 catccttctt tcaagttctt caggataagg agcaaagttt tgttgaaaat actcaagtgc
    29761 ttcatctgtc gtttgaaatt ccccaacgga agtctcaatc tccgcccaag cagattcatc
    29821 ttctttttga taaaatcgaa attcaaaacc ttccgctaaa ggcgtgattg gaacattttg
    29881 ttgaagtggt cttgtcatcc agatttctgc gtaaggtaac gttttatcta acatttttct
    29941 atccctcctc gtctccatta tgccaaataa catatgactc agctacttat ttgactataa
    30001 cagatagaga tataaatcag aaccagtcat ctgcgtcaaa ttcatttcga ttagcgtata
    30061 cccatgttcc tgccatacct cgcatgaaat ctcctaaagc agaagaagga atacctccac
    30121 caggtgcggg tgttcccgga ctaggaagtg gtacttgcgg ctgcagatgc tcatcggtgg
    30181 aatcaatagt tgacaagcgt tttttcttcg tttccttctg gtcatctgat tccttctgat
    30241 cttccgactg tttagcaaca aattcttttt ctgattgttg ttttatttta ggctctaaaa
    30301 tttcattttc caatccagaa acttgtgggg ccaaatgagc ttcttgcctt atcgttttat
    30361 tagattctga gatttttcta gatattcttg ctaactctag gcttcttgtt tgttttactt
    30421 tccctgttgg agaaactgca tgagccattt gttgtttttt ttctgacaag ctttcttctt
    30481 gtcttttttg ttctttttga tctatctgtt tgtttaatgg taaaacatgt aatagtgact
    30541 gagcatggag ttcttggagt gaatacgggt catgataact atacataatc gtaaaatcac
    30601 ctgtttgttt ggtcaaataa ccatcctcca atggtacttc attgattgta accgatacat
    30661 cctttaacgg tacgggttta cctaaggaat ttgttgctga ttggatcaac tcttctttgt
    30721 ttattttttc gtttggatga acaataagat catgaccttc taatgaacga ttgtctggta
    30781 tataaacata agtaaaggta atttcccctc ctgattgctt cctattattc atcagttctg
    30841 agtgaagtac ttcaaggatc tccgaccagt gactttttct gtctaatccg tttgcttgca
    30901 tccaatcaga aatagaagtg actttcccat tttgttcata agtcgataaa tctatatttg
    30961 gcactcgatc agaaaattca gcaggtaggc gcaaatcagg aatacgattt acactactca
    31021 tatgtgtttc tgcttgaagt tctttatttg tttgactatc tataaaatga gtgatgacag
    31081 tcgatgaagc agcagatgct tctacataaa ctacagctgt tgtttctgcc acatttgatg
    31141 ttttcgagtc tatataagtt aatacaacgg gatattctcc cggagttgta gtatctactg
    31201 tcccgttcat tttgatttct tctttatttc ctgaagttcc atcgcttttt gttacatatt
    31261 caacaagatc tttaggggcg taactctctc ctgttcttaa gtgaagatct tttcctttga
    31321 tagctgtttg atcagcacga acatggatca cagcagatgc agtcattgtt tttaaagcaa
    31381 ttggatcaaa ataggacaag gaaatagtag atgttcctgt attagtcgta tccacgatat
    31441 tcccattgtt tgaaattact ttggacatat ctccttgtgt tccatctgcg ttcttcagcc
    31501 tttctaccag atcagatatc gaaacttttg tccctgccag aacatcgatt ttctttcttt
    31561 caaactgtga ataatctttt tcatatacat aagaaacttg tgcgcttcca ccgtccttta
    31621 tatctgtcaa agcatgattc aaaatatcag tgatctgttt taactgatca ccttttgtag
    31681 gatcagtcat atcggaaaag atagaaagcg gcacactgct tccggtttcc gatgtccata
    31741 atgttttgtt aggaattaac cagtagtggt ttatcgttgg aggataactg actttattat
    31801 aggaagtcaa tgcaggaagt agctgttttc catttgcatc agtgaaagta tgagtaatag
    31861 tgatagggga atcagtggtc tcagaaacag aggtacgtac gaccttgatg gtttgtgtcg
    31921 ttgcctgaac gggtacatgc atatagacac ctacagataa actgatcagc atcgtgcgca
    31981 tcataaactt aacgtagtgt ttttcagaac acgaccgttc cttatctaaa cgacaagcgt
    32041 gtttatttag ttgttttgaa cgtctttttg aacacattta ttcttagtcc tccctactct
    32101 tgtctaaaca tgaagtagta attttcataa gatgatttca ttatataaat aaaatcaaaa
    32161 gaagatggac tattaggaga gtattttttt tgatttttta ttgtttcaag aaaatcggaa
    32221 taataactta tgtacttttt aattttgtta agtaatatat ctagttatat ctagttatat
    32281 ctagttacct tttctttgaa aaaaacataa atcagctaat aaatatgtga gacaaagctg
    32341 ccatccataa atactagctg attttttcag ttaaatgtct atttttttct ctgatgatgg
    32401 tatttccaaa atattcccag tcatctggag tcggtggatt tttccaaatt atctgttctt
    32461 tattaaattt ttcaagtaaa caatcagata aataaatatt cgtacgagaa gtgattcgac
    32521 tctcaatcac aatattgtaa tttttaaatc ctttcacttg agaaataatg taatcggtat
    32581 aagcagatcc ttgcgaaaaa tcaatacaga catgaatcgg tgcttcaaaa tatgataccg
    32641 gacaaatacc gaccaataaa aagagataat cataaaataa gcgaatcatg aaacgttcat
    32701 cttttcccaa gaaatagcgt ttattctctt gtatgacctt ttggattgct gtactgaata
    32761 ttggatacgt ctcgaagaaa aactgtagct gctttttaga cgtaaagcta gtcatctcaa
    32821 agtcgaatat ccaatttttt cgattgacat gcatcatacc catacgaagt tcttttcgta
    32881 tatatagtct ttcttcttca gagatttcaa tcgaaaatgg taattgatca atcaattcca
    32941 ctgacatttt ttgactaatc atgtggatat catgaaaatg agcttccgca agtccaaatg
    33001 cgccatctaa ttcaaattct tcttccgtga tcaaaaacaa catgatataa gaaatctcat
    33061 caatgatctg ctctttaggg atatgaagaa aagaaatatg tgataaatcc ttgatgatct
    33121 tcattccttc tgagctctta aagtattcat ctgaaaagaa acgttttccg attggttgat
    33181 tcccataggc cctcaacaaa ctaatagtgg ttagcaaatt gaatttgatt cgttttgttg
    33241 cagacagttt taaattgaac agatattcaa aatattgcac gatactcttt gcttcattca
    33301 tgacgctttc tctaaatggc atagaaaaac cattaaagac cccgaaatat accccaaata
    33361 atacgttccg tatgtttatt tcttccccaa ctaatatgtt tttctttaac tttatttgat
    33421 gctgttcaag aaaagagact aattctcttc tttttacata ggctcttgat atgcttaaaa
    33481 aatgattctc tgcaaatttt tcgatcgtta cttttgaatc cacgatctcc gttaacagtc
    33541 gagcaatttt tgatcgttga aagtagattc ttcttaaatt tttgatatcc gtcaaattca
    33601 gacgttcgac atggatctct gcttcttctg aaatttggat ctctggcttt gttttgaatt
    33661 cagtaaattc tttatttaat tctatgatat aatttcttgt tttaaattta gacaaaccta
    33721 atgtttcata taacttaggc agcactacat aattcttttt ctcaaattct aaatatctag
    33781 ctagcagcag tttttctcta tcttcctctt ctataaggta atccaacttc atatcctaca
    33841 cctcaaaaaa tgcctcatac caattacttg atatgcagat gaaagttcat cttttcacct
    33901 atactgattt tatgatggat tatgcttgtt ttttcatcag ttattctcat tttttatctt
    33961 ttttttgaga atattgcaaa aaatggaaaa atttatgagg aaatattcta tattttttgt
    34021 tttattgtgt taaaatgtac aagatttgcg ccatttgcgt aaacaaccac aaaaaagagc
    34081 aggacggcca ccgtcctgct cttttactta ttttaagttc cattacttat taattggcta
    34141 aacttctgct tgtttctgat attgcttgtt tttctccaat gatccgcact tctgtttcta
    34201 aagacacatc aaatttttct ttgattactt cttggatgtg cgcaatcaat tcaatataat
    34261 ctgtcgctgt cgcatgatcg atattgacga tgaatccagc atgtttttca gagacttgtg
    34321 caccgcccca tttcaggcct tgcaatcctg cttgttggat cagctgacct gtatagtgtc
    34381 ctactgggcg cttaaagaca cttccgcatg aagggtattc taatggctgt tttgattgac
    34441 gcaattcggt caactcatcc attcttgctt tgattgcttc ataatcccct gtttgcaaac
    34501 tgaatcgagc ttccaatacg atagcacgta attcttggac tttactgtga cgataagaaa
    34561 ataccatttc gtcttttgtc aaagttttca atgttccatc ttcaagcaaa agatccactt
    34621 cagcaaaaac atctttgatc tctccgccat acgcacctgc attcatataa accgctccac
    34681 cgacacttcc cgggatgccg caagcaaatt caaatccagt caatgattct gccaaagctg
    34741 cataagtcgt atcaatcagt gaggcacctg cttccgcaat gaccatcgtt cctttgacat
    34801 gaatctgttt catttcagtc agcatgatga ctacaccgcg aatcccgcca tcttgaacga
    34861 tcagattgct tgcatttcct aataccatcc aaggtatatc atggattcgg caataatcga
    34921 tgatttgctt tacttcttct tgttttttcg ggaatgcaag gacatcagct ggtccacctg
    34981 ttttcgtaaa cgtatagttc atcaatggtt catcaaacaa tatagtaatc gtggctaagc
    35041 tttcaactat ttcttcttta ttcactgagg attttccttt cttttctcaa acgtcctcat
    35101 tattctatca cgcttaatac aatcttgcca gtcttgttca aagttgatga cggtagtgat
    35161 ctttaagcac ttcctcacat aattcatgac ttcttacaac ttctttttgt ttaagcaatt
    35221 ctgtttccga tttacctaaa cggatctgag aaaagaaggc ctctaccatt ggctcaaacc
    35281 cgcgtttgac tagcgtgttt gtccagtcgc caaattcttt cacttttttt ccatcagcat
    35341 cttgaatgac aagctgggtc agatcgttta cttcataagt accattttta cttgttactt
    35401 gatatttttc tatattagcg ccacttgcta aatccatcgt taaaatagcc gtttgatttt
    35461 gtgtttctaa ttgtaaaatg gcatattcca aaaattcctt tgtttctctt atctggtatt
    35521 ttgttctcaa aatgggttca tctaataaat acacagctgt atcaataaga tgtaaaaaca
    35581 aatcataaag gacgaactct gtcgtttctt ttgccgctat tcgatttttt tgcaggtgga
    35641 tcaaccgctt atctgggata cgtttcaatt cttctaccat aggagcaaag cgccggttga
    35701 aaccgatcat caaaattatt tggttcttat cagctaattc ttgcaattca cgaacttcat
    35761 ttagatggac actcagtggt ttgtcaatat aaacatggat accattttct agacattttt
    35821 tggctaattc ataatgtact tttgttgctg cgtggatcat acatgcttcg attttttctt
    35881 ctaaaagctc atcgatcgtt tcatatagat gttggaaccc gtactctttt tgaattcttt
    35941 cttttgtttc tttatttctt gttgcaaaat aaaaatcaag tgttccttgt tttttcgcat
    36001 aagttggtaa atatgctttt tctgcgatat tccctacacc gattacacca atcttcatct
    36061 actttcctcc tggatcattt agttactttc atcttacacc attttttact aaaatgaggt
    36121 acaatagtat cgcaaaggta aggaaattaa ttcgaaggag tgtgctttta ttgaaattta
    36181 tctcttggaa cgtaaacggg cttcgtgcca tcgtaaataa aaatttttta gaagtttttc
    36241 atgagttaga cgccgatttt ttctgtctgc aggaaacgaa actccaagca ggacaaattg
    36301 atttagatct tccaggttat tatcaatatt ggaattacgc ggaacgaaaa ggctattccg
    36361 gcacagcgat ttttgctaaa aaaccagcat tgaatgccac ttacggaatg ggtatcgata
    36421 tccatgatac agaaggccgt ctgattactt tggaatattc cgatttcttt cttgtaacct
    36481 gttacacgcc aaattctcaa agcgaattaa aacgtttaga ttaccgatta gaatgggaag
    36541 aagcctttta taattatttg gaaaatctaa aaaaacaaaa accagtcatc gtgtgtgggg
    36601 atctaaatgt cgctcatcaa aagattgatt tgaaaaactg gaaaacgaat caaaaaaatg
    36661 ctggattcac tccagaagaa agagcggctc tttctcgact gttggataac gggtttatcg
    36721 atacattccg ttatttttac ccaacccagg aaggtgtcta ctcttggtgg aactaccgat
    36781 tcaattcaag gaaaaacaat gctggttggc ggatcgatta ctttttgacg agcaaagatt
    36841 tagagccagg actagctgat gcaaagatcc atacggacat tatgggcagt gatcattgtc
    36901 cagtagaatt agatttgaaa taaagaggtt gtgaaaaagt cgtttagttt cgagtattaa
    36961 gaaaaatttt gtgaaaatgg ctttccacat tttgcataaa tttggcataa taccgagaaa
    37021 ctggcttttg aacactgttt atcgagagat tgtgaaaaag ctgtcctgca tcaagtaata
    37081 agaacagcca aacaaaaata gttcctcata tttttcgttt ggttgggctt attaccgcag
    37141 atacaagctt ttgaacaccg tttattaaga ggttgtgaaa aagctgcact gcatcaagta
    37201 ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg
    37261 cagatgcaag cttttgaaca ccgtttatta agaggttgtg aaaaagctgc actgcatcaa
    37321 gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt ggacttatta
    37381 ccgcagatgc aagcttttga acaccgttta ttgagaagtt gtgaaatcag cgtcctgacc
    37441 tgagtattag agaaacaaat ggcaaaatag ttcctcatat tttgctattt tttgagctaa
    37501 taccgagggt cagctgattg aacactgttt agtggagagg ttgtgaaatc agcgtcctga
    37561 cctgagtatt agagaaacaa atggcaaaat agttcctcat attttgctat tttttgagct
    37621 aataccgagg gtcagctgat tgaacaccgt ttagtggaga agttgtgaaa aagtcgccta
    37681 ctttcgagtc gtaagaaatt tttttgaaaa tggttcctca caactttgca taaatttgac
    37741 ttaataccga gaagtcatga tccccatcta actccaaatc acaaggaatg agttcaaaaa
    37801 ctagcttttc tcaacaaata ttacataaaa aattctatgt tttttgttaa atttatttgc
    37861 ttttttcaaa agtatgttac agtaatttcc caaggaggtt ttacagaaat gaattttgtt
    37921 gccatggatt ttgaaactgc caatcatcag ccttatagtg cttgttctct tgcgctagtc
    37981 atggtaaaaa atagccagat cgttgatgaa ttctatacat tgatacagcc tgagactcct
    38041 tttttctggc gaaatgtcca aattcacgga atccatcaag aagacgtacg caatgcgccg
    38101 aaattcccag aagtctggca aactatccaa ccatattttc aaaaaaatcg tttagtcgtt
    38161 gcacataatg ctgcttttga tacaaaggtc ttagcgggat gcttagatta ttatcatctt
    38221 gaacagccta attatctttc tttatgtaca gtgaaaagta gccgtcgcct gtttcctgaa
    38281 tttccaaatc atcgattgaa cactgtgtgc gaaaatttga acatccaatt agatcatcac
    38341 catgatgcat tggaagatag tcgtgcatgt gcggaaatcc tgctttatca agaaaaaaaa
    38401 ttcgggacag atccattgaa aaaacttgtg actataaagt aaaagaatac ttatgaaaca
    38461 cacaaagagg tcgtgagcct gccgtttcac tccgagtcac aaggagcaat ttcaaaaaat
    38521 agcttctcat gtttattaaa tattgtgact ggtgaccgag gagctggcag gagaacaccg
    38581 tttattggag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa
    38641 aatagcttct catatttttc atttggttgg acttattacc gcagatgcaa gcttttggac
    38701 accgtttacc aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaacgg
    38761 caaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa
    38821 caccgtttat tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt
    38881 ttttctgcct tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat
    38941 caaataactc attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg
    39001 catgaccgct tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg
    39061 aacttgaacg tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat
    39121 ttcttctagc ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc
    39181 gacttctccc acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat
    39241 aatcgtccct tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc
    39301 caactcttct tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa
    39361 ttctgtctcc ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac
    39421 tgcttcccgc aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa
    39481 ttcgctgttt gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt
    39541 ctaaatctgt atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt
    39601 ctaatagatt tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc
    39661 ctaaatctgc tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac
    39721 cttgactata ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta
    39781 atccttgggc aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata
    39841 tcagattgtc acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg
    39901 ttaatcctga tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta
    39961 tatcgcgtaa tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt
    40021 ttttggctag ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga
    40081 taatttatat acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa
    40141 gccttgtaga attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc
    40201 gatatttcct gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga
    40261 atctggcgct ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc
    40321 gtcgatcatg tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc
    40381 tacttctggc gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc
    40441 gatatcgaac aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc
    40501 agttggattc acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg
    40561 taccatgatc atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag
    40621 tgctgggcga acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat
    40681 ccccatatac gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg
    40741 ttctttggat gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt
    40801 atcgtaatta tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc
    40861 tgcatttctt atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt
    40921 caaccgggca gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat
    40981 cccacggcga acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga
    41041 cgtttttata tgtattatca tacactattc cagaaaaaaa gaacagttta ttttatctgt
    41101 atcagtcaaa aatgctggaa aaattaagtt tttcacatac aaagcgaact gcactataac
    41161 aggaaagcgc tgttttatta cactgtttca ggcagttgcc aatcaatcgg ttcttctcct
    41221 aacgcaagga gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa gccacgactt
    41281 gcagataacg gactgggatg tggagatgtt atgatgatat ggcgactctt atcgatcatt
    41341 ttgatttttt cttgtgccgg ttttccccac aagataaaca cgattggttt ttcccgttca
    41401 tttaattttt caatgatcgt gtctgttaat ctttcccaac ctttcccgcg atgagaatac
    41461 gcttgccctt cccgaacggt aagaacggtg ttgagtaaaa ggactccctg tttagcccat
    41521 gacactagat ttccatgttg aacaggtgcg attccaagat catcataaag ctctttatag
    41581 atatttctta acgatggcgg gatctttacg ccaggttgca cagaaaaaga taagccatga
    41641 gcttgatttt ctccatgata aggatcttgt cctaaaatca ccactttaac ttcttcataa
    41701 ggagtcaatt ccaatgcttc atagatatga tacatatctg gatggatctt ttgggacgca
    41761 tattcttttt ttaaaaattc tcttagatgc agataataat cttttgaaaa ttctgaggac
    41821 aaaacttctt gccaactgtt atgaatgatt tctttcaaaa actccacctc ctaataaaaa
    41881 gaataactca cattttcttc aatcacaagg gacaatcttc accctcagca cttgattaat
    41941 atggtaaact aggaacaaga agcagaaagg aacgataatc attatgatca aattaattgc
    42001 atcagatatg gacggcacat tattagatgc ccatatgagt gtttctcctg aaaatgccga
    42061 ggcgatacgt tttgcaaatg atgccggtgt tgagttcatg gtcgctactg gtcgaaacta
    42121 tcaagaagct cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga tcactttgaa
    42181 cggggcacag gtatttgata aagaaggaaa ttcattgttt accgtgtcga ttccaaataa
    42241 tcaagcaatc tctgttttag atattctaga tgctaacggt atttactatg aagtagcaac
    42301 caacgatggt ctttattctg aaaatcaagc gaaacgaatt gaaagttttg cttccatggt
    42361 ggctacccat ttacctcacc ttacgtataa aatggcgatt gccatggcct cagctaagct
    42421 ggagttattg cacatcacat acgtggacag tattcgtgct attttggaag aaaagaatct
    42481 ggaagtttta aaaattattt gttttcatac agaagggcca gctgtattag gtccagttgg
    42541 aaagcagatt tctcttttag gcgatctagc cgttacttcc tctggtcaaa acaatatcga
    42601 agtgaaccat aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg
    42661 gatttcttta gaagaagtca tgacgattgg cgataacttc aatgatgtca gcatgctgca
    42721 agtagcagga gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag attatgccaa
    42781 atatacaacg gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc
    42841 agaagacttg tagttttatc tagaaaagag ggatatagat gtcagaattt ttcattcagg
    42901 aaaagcagct tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt
    42961 ttttgatggt tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat gccatgaatg
    43021 gcgatctcgt tgctcacatc aagcagataa gcctgacatt cggtacgcgt tttgagctgt
    43081 ataaagagtt cgaaaaggtg ggaaccatgc agaaaatatt caattggccc ggtgactttt
    43141 attatattcg tagacttcac tggacagtcc aaggtgatat ctataatcat caatatcaaa
    43201 tccatcattt caatcagcaa atcatgaaaa tggataaagc aacacttttt tcaggagatt
    43261 actatgtatt ggatgtctca aatgatgaag atgctccagt ttgtatttgt attgctgcgg
    43321 ttttagatta ctggctctac cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac
    43381 ttaacttaag ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag gtacatcttt
    43441 t
//
LOCUS       DASHFF010000018        42580 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.018, whole genome shotgun sequence.
ACCESSION   DASHFF010000018 DASHFF010000000
VERSION     DASHFF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.018"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            287..694
                     /locus_tag="U2I75_001106"
     CDS             287..694
                     /locus_tag="U2I75_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696253.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="U2I75_001107"
     CDS             708..1109
                     /locus_tag="U2I75_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696254.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="U2I75_001108"
     CDS             1111..1482
                     /locus_tag="U2I75_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696255.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="U2I75_001109"
     CDS             complement(1518..1820)
                     /locus_tag="U2I75_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696256.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2069..2269)
                     /locus_tag="U2I75_001110"
     CDS             complement(2069..2269)
                     /locus_tag="U2I75_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1696257.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2574..3806)
                     /locus_tag="U2I75_001111"
     CDS             complement(2574..3806)
                     /locus_tag="U2I75_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN1696258.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4060..4629
                     /locus_tag="U2I75_001112"
     CDS             4060..4629
                     /locus_tag="U2I75_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1696259.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4807..5247)
                     /locus_tag="U2I75_001113"
     CDS             complement(4807..5247)
                     /locus_tag="U2I75_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN1696260.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5405..6169
                     /gene="map"
                     /locus_tag="U2I75_001114"
     CDS             5405..6169
                     /gene="map"
                     /locus_tag="U2I75_001114"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN1696261.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6201..7124
                     /locus_tag="U2I75_001115"
     CDS             6201..7124
                     /locus_tag="U2I75_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN1696262.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7200..8339
                     /locus_tag="U2I75_001116"
     CDS             7200..8339
                     /locus_tag="U2I75_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN1696263.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8332..9132
                     /locus_tag="U2I75_001117"
     CDS             8332..9132
                     /locus_tag="U2I75_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1696264.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9132..9959
                     /locus_tag="U2I75_001118"
     CDS             9132..9959
                     /locus_tag="U2I75_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1696265.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9937..10671
                     /locus_tag="U2I75_001119"
     CDS             9937..10671
                     /locus_tag="U2I75_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1696266.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10771..11637
                     /gene="rfbA"
                     /locus_tag="U2I75_001120"
     CDS             10771..11637
                     /gene="rfbA"
                     /locus_tag="U2I75_001120"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN1696267.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11651..12223
                     /gene="rfbC"
                     /locus_tag="U2I75_001121"
     CDS             11651..12223
                     /gene="rfbC"
                     /locus_tag="U2I75_001121"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN1696268.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12245..13273
                     /gene="rfbB"
                     /locus_tag="U2I75_001122"
     CDS             12245..13273
                     /gene="rfbB"
                     /locus_tag="U2I75_001122"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN1696269.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13371..14222
                     /gene="rfbD"
                     /locus_tag="U2I75_001123"
     CDS             13371..14222
                     /gene="rfbD"
                     /locus_tag="U2I75_001123"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN1696270.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14257..16290
                     /locus_tag="U2I75_001124"
     CDS             14257..16290
                     /locus_tag="U2I75_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696271.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMITFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16334..17614
                     /locus_tag="U2I75_001125"
     CDS             16334..17614
                     /locus_tag="U2I75_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN1696272.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17824..18630
                     /locus_tag="U2I75_001126"
     CDS             17824..18630
                     /locus_tag="U2I75_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696273.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18642..19862
                     /locus_tag="U2I75_001127"
     CDS             18642..19862
                     /locus_tag="U2I75_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1696274.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19852..21438
                     /locus_tag="U2I75_001128"
     CDS             19852..21438
                     /locus_tag="U2I75_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1696275.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21477..23615
                     /locus_tag="U2I75_001129"
     CDS             21477..23615
                     /locus_tag="U2I75_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1696276.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(23983..24966)
                     /locus_tag="U2I75_001130"
     CDS             complement(23983..24966)
                     /locus_tag="U2I75_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN1696277.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            25344..26735
                     /locus_tag="U2I75_001131"
     CDS             25344..26735
                     /locus_tag="U2I75_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1696278.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            26751..27791
                     /locus_tag="U2I75_001132"
     CDS             26751..27791
                     /locus_tag="U2I75_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1696279.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRKIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            27810..28895
                     /locus_tag="U2I75_001133"
     CDS             27810..28895
                     /locus_tag="U2I75_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN1696280.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            28928..29890
                     /locus_tag="U2I75_001134"
     CDS             28928..29890
                     /locus_tag="U2I75_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1696281.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            29883..31310
                     /locus_tag="U2I75_001135"
     CDS             29883..31310
                     /locus_tag="U2I75_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN1696282.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            31312..32382
                     /locus_tag="U2I75_001136"
     CDS             31312..32382
                     /locus_tag="U2I75_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696283.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            32369..33790
                     /locus_tag="U2I75_001137"
     CDS             32369..33790
                     /locus_tag="U2I75_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN1696284.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            33803..34810
                     /locus_tag="U2I75_001138"
     CDS             33803..34810
                     /locus_tag="U2I75_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1696285.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            34822..35826
                     /gene="neuB"
                     /locus_tag="U2I75_001139"
     CDS             34822..35826
                     /gene="neuB"
                     /locus_tag="U2I75_001139"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEN1696286.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            35823..36971
                     /gene="neuC"
                     /locus_tag="U2I75_001140"
     CDS             35823..36971
                     /gene="neuC"
                     /locus_tag="U2I75_001140"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEN1696287.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            36944..37621
                     /locus_tag="U2I75_001141"
     CDS             36944..37621
                     /locus_tag="U2I75_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1696288.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            37611..38753
                     /locus_tag="U2I75_001142"
     CDS             37611..38753
                     /locus_tag="U2I75_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN1696289.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            38766..39743
                     /locus_tag="U2I75_001143"
     CDS             38766..39743
                     /locus_tag="U2I75_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1696290.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            39743..40651
                     /locus_tag="U2I75_001144"
     CDS             39743..40651
                     /locus_tag="U2I75_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEN1696291.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            40652..41404
                     /locus_tag="U2I75_001145"
     CDS             40652..41404
                     /locus_tag="U2I75_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN1696292.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            41409..42356
                     /locus_tag="U2I75_001146"
     CDS             41409..42356
                     /locus_tag="U2I75_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN1696293.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        14299 a         6445 c         8239 g        13597 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata
     2041 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
     2101 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
     2161 tagatgcatg cgtatgtctt agtccatcaa taaacagtcg aagagaaaat agggaaaaat
     2221 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaataga
     2281 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccaacgaaag atatatcgtt
     2341 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
     2401 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
     2461 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
     2521 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
     2581 caatcgccgt ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
     2641 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
     2701 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
     2761 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
     2821 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
     2881 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact
     2941 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
     3001 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
     3061 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
     3121 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca
     3181 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
     3241 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
     3301 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
     3361 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
     3421 agtgctttgc cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat
     3481 gcatcgggat cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc
     3541 gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga
     3601 aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta
     3661 taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca
     3721 actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt
     3781 agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa
     3841 ctattagcat gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga
     3901 ggttattttt ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg
     3961 aagaaaatca aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat
     4021 ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt
     4081 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
     4141 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
     4201 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
     4261 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
     4321 atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt
     4381 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
     4441 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
     4501 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
     4561 attgaaaaga tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa
     4621 atcgattgaa tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat
     4681 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
     4741 gaatgcaatg aataaaagag ccatacagat tgaataaatc tggatggctc ttttattgca
     4801 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
     4861 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
     4921 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
     4981 tccgcagaca ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc
     5041 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
     5101 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
     5161 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
     5221 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
     5281 tatgaattat accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg
     5341 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
     5401 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
     5461 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
     5521 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
     5581 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
     5641 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
     5701 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
     5761 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
     5821 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
     5881 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
     5941 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
     6001 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
     6061 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
     6121 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
     6181 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
     6241 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
     6301 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
     6361 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
     6421 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
     6481 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
     6541 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
     6601 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
     6661 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
     6721 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
     6781 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
     6841 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
     6901 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
     6961 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
     7021 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
     7081 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
     7141 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
     7201 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
     7261 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
     7321 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
     7381 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
     7441 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
     7501 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
     7561 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
     7621 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
     7681 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
     7741 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
     7801 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
     7861 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
     7921 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
     7981 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
     8041 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
     8101 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
     8161 cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
     8221 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
     8281 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
     8341 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
     8401 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
     8461 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
     8521 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
     8581 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
     8641 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
     8701 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
     8761 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
     8821 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
     8881 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
     8941 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
     9001 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
     9061 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
     9121 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
     9181 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
     9241 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
     9301 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
     9361 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
     9421 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
     9481 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
     9541 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
     9601 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
     9661 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
     9721 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
     9781 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
     9841 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
     9901 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
     9961 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
    10021 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
    10081 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
    10141 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
    10201 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
    10261 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
    10321 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
    10381 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
    10441 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
    10501 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
    10561 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
    10621 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
    10681 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
    10741 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
    10801 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
    10861 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
    10921 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
    10981 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
    11041 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
    11101 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
    11161 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
    11221 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
    11281 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
    11341 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
    11401 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
    11461 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
    11521 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
    11581 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
    11641 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
    11701 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
    11761 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
    11821 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
    11881 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
    11941 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
    12001 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
    12061 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
    12121 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
    12181 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
    12241 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
    12301 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
    12361 aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga
    12421 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
    12481 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
    12541 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
    12601 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
    12661 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
    12721 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
    12781 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
    12841 tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
    12901 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
    12961 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
    13021 cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa
    13081 cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct
    13141 aggttggcag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
    13201 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
    13261 aaatggacaa taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag
    13321 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat
    13381 taactggagg aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc
    13441 tgccatatgt ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg
    13501 cttatattac agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag
    13561 ataaagcaga agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa
    13621 atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg
    13681 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
    13741 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
    13801 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
    13861 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
    13921 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
    13981 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa
    14041 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
    14101 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca
    14161 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
    14221 aatttccttt ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca
    14281 cgacgaaaag aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt
    14341 ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta
    14401 aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa
    14461 actgatgtga tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca
    14521 cctttttacc tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta
    14581 aacggacaaa acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga
    14641 aaaccattca actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg
    14701 gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag
    14761 aggaaaaaag ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag
    14821 tggtggttca tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattact
    14881 ttttatcatt atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg
    14941 gtggttattt tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg
    15001 gcgtacttgc tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca
    15061 tgggatctgt tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg
    15121 gttcatttct ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca
    15181 ggaaaacggg taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca
    15241 aattggaaaa ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc
    15301 tatcactttt ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat
    15361 agcgaacaaa agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg
    15421 attaccgttg ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca
    15481 ttaagagcca tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct
    15541 tatatttgga gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat
    15601 gcatggtctt atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt
    15661 gaaaccatcg cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg
    15721 agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat
    15781 ccagtcgtag aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa
    15841 atcgatcaac aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc
    15901 ccaacattgg gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg
    15961 aagaaaatcg atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga
    16021 gcttttatcg ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc
    16081 acgttgaatt ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca
    16141 ttaactgatt acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa
    16201 gacggttata tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa
    16261 actcaggaaa atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt
    16321 agtagaaagg agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag
    16381 tgtcggtttc ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg
    16441 ttttccagca actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc
    16501 tattttgctc attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt
    16561 tgcgcttgcc cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt
    16621 tggagactta ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg
    16681 gacaaaatgg gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt
    16741 atatcgtgta tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa
    16801 ttcgctttct gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc
    16861 tttattgatc agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg
    16921 gatagcagtc gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt
    16981 cgcgttgatt gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg
    17041 tgcgccattt attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc
    17101 ggctgtctct gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta
    17161 tcagacgcta ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca
    17221 gcgaggaaac caatttttag gaaaccacaa tagttataat tctattttga atatctatgg
    17281 attgatagga tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt
    17341 gaaagcagat ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat
    17401 gcagtcattt atggaggata cattgacttc ataccgatgg gttccgatta tttatatcct
    17461 gttggctatg gccgttcatc gatatgatga accagaagtt acggaggaat tctatgttat
    17521 ggaagaggca tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag
    17581 cgctcattct tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt
    17641 gacaaccgtc gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga
    17701 aacgttagag aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt
    17761 accaaaaata tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca
    17821 caaatgttta aagatttgta tttatttatg aaggacattt atcagaacag aaaattactg
    17881 ctacagtttt cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta
    17941 tgggcgttta tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga
    18001 ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg
    18061 attccctggt tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat
    18121 acttatttag taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta
    18181 tccaatctgt acacccattt attcttcatt ctgattggca tagctgtcgc gcttataaat
    18241 ggtatatatc cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct
    18301 ttcttgacag gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg
    18361 caattaatta cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct
    18421 aatcagtttg attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt
    18481 caaggttatc gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact
    18541 ttgtatttct gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga
    18601 ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta
    18661 gactaaaaga tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg
    18721 aagccctcaa tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga
    18781 atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat
    18841 ctacaatgtt gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg
    18901 aaggaaaaat ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct
    18961 atgaaaatat ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa
    19021 tggtcgatga tatcattgaa tttgctgaca tcggcgacca tatttatcaa cctgtaaaaa
    19081 cctattcaag tgggatgttc gttcgtctag cttttgctgt agcgatcaat gtgaatcctg
    19141 atattttgat tgtggatgaa gcattggcag taggagatct tgaatttcaa ctaaaatgta
    19201 tggaaaaatt tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata
    19261 ttagttcgat tcgccgtttt tgtgatcgtt cctattggtt gaaaaatgga aaagttgtcg
    19321 aatatggtga tacgatggaa gtaacgacga attacgaaaa ctttttgaag aaaaaatcag
    19381 ttaaaacagt agatcgaaat aaaaaccaag tagaacgctt cgcagctcca gatatcgtag
    19441 acgtgacgaa agccgaattg ttggataaag ataaaaatcc agtggatatg ctggaacaag
    19501 gagaaaaact atacgttaaa gtaacctatg aagtaaaaga cgatacaatc aaaaatccag
    19561 ttttaggaat tgctctgcgt acagtcgaca atttttatgt gtgcggactc aatactctac
    19621 tagatggtat caagatccct tggaaaaaag gtgagaatat cttttatctg gagtatgaaa
    19681 agttaggttt gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa
    19741 cggttccttt ggtatacaag acaaaataca tgaacttgtt tgtcagcggt tcttatatcg
    19801 gagaaggcat cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat
    19861 gattttgaaa tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa
    19921 ccaggaagta aagttttaga gtttggtcct ggaaacggcc gattaacgaa acatttgatc
    19981 ggagcaaaac aatgtgaagt gagcattgtc gagctagata aggaattgtt cgattttgtc
    20041 agcgagtttg cccaagatgg attttatgga gatatcgaaa gttttgaatg ggcaaactat
    20101 tatgctggac aaacatttga ttacgttttg tttgccgatg ttttagaaca tttggtagat
    20161 cctggaaaaa cgttgaagaa agttagagaa tttttgaatg aagaaggaga aattctaatt
    20221 acattcccta accttgcgca taattctgtc atgattgatt tgttcaataa tcaattgcca
    20281 tgggcttctt atggtttgct agatgaaacc cataattctt tttatactca tgatggcttc
    20341 caaaaagttt ttgaaaaagc aggattatac atcaatattg aagattattt gtacttagct
    20401 gtaggtgata cagaattaaa atcaacttat gaagaattgc cggaagctgt acgttatgat
    20461 ttcaaaatgc gaccatttgg cgaagtctac caatacttct tctcattgat gaaacatcca
    20521 gtagcacaaa gttccattgc gcagccgcaa aattctaatt atgtgaaagt attagaagta
    20581 atccagcaaa cgaaacaaga tgaaataatt caacaaatac cattcaataa tttcacaggg
    20641 gaaaatgaga cactaagttt ccatgtatca gaattagcag aacgaatggt gtttcgcttc
    20701 gcgcagcaac ctagctttat cgaattcagc gcagaagctg ctggagaaaa actcacattt
    20761 atcgattcaa atgctgtagt caaaacagta aatgattgtt atttgtttga tggtaaagat
    20821 ttaccagagt tctttttaac ggatattgca gggaaagaag tgactatcca ttgtcactat
    20881 cgttttatcg gagaattgac accaacaatg aaagaattgc tggaaacaat ccgtccaatg
    20941 gctgaagtta caaagcagct ttctgagaaa aatgatgaat tagaaagaga aaatcattat
    21001 cttattgaga ataatacaag attagatcag acgttgaaaa caaccataaa tcgctattgc
    21061 acgttgttgg aaagtgatga atggacaatc aaacatagat tagggcgacg taagaaagaa
    21121 acatcgaaaa aaatccaaga aaaagagctt tctctatgta ttgacgaaaa aatctgggat
    21181 gcagaaacaa agatattgaa aatcatcggg tggggaatcg ctaattcgga tcgccaacca
    21241 ttgtcctata agttatcagc cgaccaatca ccattttttg aagcaattcc tgttttacga
    21301 gaagaagtca atcaagcaaa acagctagct gaaggagcaa aagcaggatt tgaactgcgg
    21361 atcctttgcg agaaggaacg ttcattctta gttgaagcag tggctcaaaa tggtcaatca
    21421 tggatcattg aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc
    21481 aaaaaaaaga aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt
    21541 tgcttattgt gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag
    21601 aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat
    21661 ataatcttga tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag
    21721 gaaaagcaat cttagacttc caaacagcaa gacatcaaaa tggcatagct gtgaaattaa
    21781 acggtaaata tccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac
    21841 tgaaaaaagg gataaattat gcaaggactc atggcgtaaa aaaagcaatc cgccgggtca
    21901 aactggaatt gaaaccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac
    21961 ctgatctgaa agagcaaagg aaagaagtac aaggatttgc ctaccgccca ttgatttcca
    22021 tacttatgcc tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac
    22081 ttgatcaaac gtatgatcac tgggaactat gtatcagtga tgatgcttct actgatccag
    22141 ctatccgaaa gtgtctagaa tcctatcaag caaaagatga tagaattaaa gtcgttttcc
    22201 gacaagaaaa tggacacatc agcctggcaa ctaattctgc attggaaatg gcagaaggag
    22261 agtttatagc gctcttagat aatgatgacg aattacctcc atttgcctta tatgaagtgg
    22321 caaaagtgct aaacgtacat ccagaactgg atttgatcta cagtgacgaa gataaaatcg
    22381 atgcagatgg gaatcgcttt gacccgcact tcaaagcaga ctggtctccc gatacattga
    22441 tgggaaataa ttatatctct cacttaggcg tatatcggac aagcattgtt aaggaacttg
    22501 gcggtttccg taaaggatat gaaggctctc aagattatga tttggttctt cgtgtgacag
    22561 aacaaattcc tgcagaacac atttaccaca ttgaccgtgt cttgtatcat tggcggacga
    22621 tcccgggatc aactgcaagc aatggagaag ctaaaagcta catctatgat tctggagtga
    22681 aagcattgac agatgctcta tctagaagaa atatcaaggg aagcgtacat cctggcagaa
    22741 tttctggttt ttatgaaatc acttatgatg ttttgcaaga agacttggtc agtgtgatca
    22801 ttccaactaa gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa
    22861 cgtcttatcc aaattatgaa atcattattg cagataatgg aagtacagat cctaaaatgc
    22921 aagaattatt tgcagaatac aagcatcagc tgaaagaccg atttatcgtc gaattaattg
    22981 acattccatt caattattca cgtatcaaca atctggcagc agaaaaagca aacggcaaat
    23041 actttttatt cttgaataac gatacagaag tgattgaacc tgactggatg acagcgatgg
    23101 tttcttatgc acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg
    23161 atacaacgca gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga
    23221 ataactatga tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact
    23281 tagcagtcac tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt
    23341 ttgatgaaac attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac
    23401 tgggtcgtta caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa
    23461 gaggttatga agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc
    23521 aagataaatg gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag
    23581 aaggaatcgg cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg
    23641 tgattcgctc gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc
    23701 gactcaaaaa aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa
    23761 attcacgaaa attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct
    23821 tctgctccca ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc
    23881 ccacgctctt attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg
    23941 aaccctagct gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg
    24001 tttaaggtat tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt
    24061 gatggagata accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat
    24121 tgctgaatat atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt
    24181 ttgtaagtct tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg
    24241 tacgatttgt tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag
    24301 atcacttgcc gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa
    24361 ccagacattg gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc
    24421 agcataataa ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa
    24481 atcaacagtt gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac
    24541 agtttgatag ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt
    24601 ttttgtccaa tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg
    24661 tgctttcatt gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt
    24721 cactttatac tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg
    24781 ctctaaagca atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca
    24841 agtatcgtct tttttataac cattccattc atctgtataa acttctaccc catccttata
    24901 tgcgacaatt tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt
    24961 attcataaga tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca
    25021 attttttcga ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag
    25081 atggattctc tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata
    25141 cttcgacttc tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt
    25201 tagagtcctt gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca
    25261 ttacaatttg cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt
    25321 attttgtgat taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga
    25381 tattcattat attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac
    25441 tgaagtttcg tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta
    25501 ttatttcagt gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc
    25561 gaatgatcag tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga
    25621 tcatggtagt gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt
    25681 catttgttct cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt
    25741 tacgcgtaag tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag
    25801 caatccaaaa ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg
    25861 ataactatta tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt
    25921 ctcatatcga agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa
    25981 aattgttttt gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt
    26041 tcgaagatga aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat
    26101 tcaaacgttt gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta
    26161 tgttattcac agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag
    26221 tgcggatcac aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca
    26281 cagccgaagc aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg
    26341 taggtaaatt tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga
    26401 aaggcgatat gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca
    26461 atgcagaaaa cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg
    26521 cacaagtcta tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt
    26581 atatcaaaaa atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc
    26641 tatttgataa aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag
    26701 aaattgcttt tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc
    26761 cgaaattcag cataatcgta ccagtataca atgcagaagc aactattggt cgaaagattg
    26821 atattctaaa agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa
    26881 cagatcaaac acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga
    26941 ttactacgac caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag
    27001 gagaatatct cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt
    27061 atgatcattt gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc
    27121 gtacaatgga cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact
    27181 atctaactca cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt
    27241 atgttgtatg gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta
    27301 aaacttataa tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc
    27361 aagtgatcat gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca
    27421 ctaatcaata ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata
    27481 ctgtaacaga cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt
    27541 cagttatttt ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag
    27601 caaagcagat attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt
    27661 cttcagcaaa aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg
    27721 ctgtaaaagt tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc
    27781 gagtatatta gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa
    27841 tgggtttagg tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa
    27901 actatcaatt tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag
    27961 agattgaaag attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta
    28021 aatatttaat agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc
    28081 aggtttattt tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga
    28141 gaattgctca tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat
    28201 tgcgtaaatt tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc
    28261 aagaagctgg tgaaagtctg tttcaaaacc atccctttga gatagttcat aatggtattg
    28321 atttagagct ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata
    28381 tctcaatgag tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat
    28441 cttatttgtt agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt
    28501 tagtaggtga agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag
    28561 agaaaagagt aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg
    28621 atgtttttgt aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag
    28681 cagcaaaatt aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata
    28741 atgtggattt tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag
    28801 agccgtatgc taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg
    28861 aacaaatgga gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg
    28921 attatttttg aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag
    28981 attggtcaat cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga
    29041 tatagaacca tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa
    29101 aaaccgtgtt aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt
    29161 aaagagagca ttacaaaatg agaaatattt aaaatatgta ttattatctg gtgctgatta
    29221 tccaatcaaa gataatgagt atatttataa ttattttaaa aagaatagtt cagtggagtt
    29281 tattcgtgga atagatctag atcaaattaa acataaagaa ttttattata aacatataga
    29341 tgtatatcaa aaacatgatt atcctagaat taataggaat aacacaactg catttaaaat
    29401 ttttagagct ataattaatc gttgtctgag gatgattaaa cttcccccaa aaattagaca
    29461 ccataaattt gatttgtatc atggttctca atggtgggct ttgtcgaaag aatgcctaac
    29521 agaattaata caaatgtatg aacaaaatca acaagactac ctgaatttta aaataggtat
    29581 gtttgcccca gatgaaaaat tttttcatac actttttttt aattcctcgt tcaaaaataa
    29641 aaatgttatt ggaggaccag atttaccact tgaattgaaa aatattgaag aaactacttt
    29701 acaaacttcg aaattagcaa atattcatat tattgatcct tccatgaaca aatggtttga
    29761 tgaaagggat atagatcaaa taaaagaaag tgaaaaatta tttgtaagaa aagttaaatc
    29821 ggattactct gataaactat taaatagaat tgataaagaa attttgaaag ttggaaatat
    29881 agatgaatag aatagtacct gttttagaga agagtttatt aagtcgagat gcgtttcgag
    29941 taataaattt aatcatttgt ctgctttatt ttatgcctag tacttttaat gtaactaata
    30001 tcccgatgaa aatagctttt gcttggggtg gaattatagt tttgtatgag gtttttataa
    30061 agaaaaattt ttttaaaatg cgtttttctt ggctactgat tggagcaatg attagtttta
    30121 tcttaactat aatcattaac agagaaaatt ttttaattcc atctatttat aattttggat
    30181 atttgctgat tactttgata gtaatttttc ctgcagactt ttcacaaagt gaagaggaaa
    30241 aaaagaaaaa aatggtgtat tttaataatg ttttcattat cataattttt ttagctgcgc
    30301 taatatctat ctatcaattt atttttttga tttcttatca tgtcccaaca ggaacgccag
    30361 gattacttgc aagacaaggt tttattgaac atcggttatt tggtgtctat acaagtccaa
    30421 atgttggagc tatgtttgga tatacttcta ttattttaag tttaattagt gtattaattt
    30481 caaaaataac aaaaacgatt cgtgtttttt atatgatgaa ttttatcgta caaatatttt
    30541 atattacact ttcttcgtct agaggtgccc aggtagtaat ttgttctttt ttagtattat
    30601 ttcttttgtt gtttgccaat tcaaaattta gaaagagcat tagagaagta ctacctgtaa
    30661 aaatcaaatg gatggtaagc atattattag ttctattggt atatttttct tttggtacta
    30721 attatgctaa ggatttttta gcaacaattc ctgtatcgat tgatagaaaa gtagaagtta
    30781 tagagtcaaa tgaatcagat aaaactccac ttccagaaaa agaagtaata ttacagcatt
    30841 ctagtgataa ctccgaaatt tcagctggtc gttttacgat ttggaaagct gcgcttaaag
    30901 ttataagacc aactttgtta tttggttatg gagagaccga tttctatggg aatagcgatg
    30961 caaaattttt gaaaaaagta gatcttgata ctacagatgt gaatgaacta aaaagagcac
    31021 atggaaacat gcataatgga tttcttcaag tacttgtatc aagtggtata attgctttta
    31081 tatgtatcta tgctttctat gtgttaaata tctttagttt actcaaaaca ttttttagaa
    31141 gtaaagtgaa ttatgcgttt cttggtattg ttttaatttt tatactatct atttttatag
    31201 atgaaatggt ggaggctcat gtacttttca ataagcgcga tgtcatttca attgttttct
    31261 ggtattattt gggaagcatt tcttacttgt atttaaaaaa ggagaattaa tatgaagaca
    31321 gctttcattg cttggactcc tttacatttg attaatatat taaatgttta tcaaaatttt
    31381 taccgagaat caaaaggaga tttatttatt tattctgaat ttgaaaacgc tagaagatat
    31441 agtgagcgta taaaagaatt agggctattt aatgatgttg tttttgtgaa tcaccggaaa
    31501 atcggttctt cctttaaacg taaaattaac ttgttaacta accataatca atttttctcg
    31561 gatgatttat ccgtttatga tgaaattttt atacaggggg aaaattattt cacaaaaata
    31621 cttttctcca atgtttacaa aaaaaatcca gatgtaaaac taaattatat agaagatggt
    31681 ttgggtactt atttgaagaa gagtatattt tcaacaagta aaaacgttca aagagttttt
    31741 aaaatattaa ataaaaatag catcttcaaa aaaaaaattg acgattattt tgtctattgt
    31801 ccggaattgt tactttatga tgtaaaaaat ataaaaaaaa ttcccaaaat agatgaaaac
    31861 agtaaattga tgaaaaacat acaatcaatc tttgggatag aagaagtttt tgtcggagat
    31921 aaatgtatct tttttgatca acctttatta tctgatggtt atggcataga tgaaacagat
    31981 attttcgcta aagtaaatag tatattttct ggaaaaaaag aaactttaat taagcttcat
    32041 cctagagcaa ggacaaatcg ttatggtgaa aaaaacatat tgaattccag tttaccgttt
    32101 gaagtcttgc tacttgaaaa tgacttctct tcaacacttt tgattagtcc tatatctaca
    32161 atttcttttt caccatctat gatgtttgaa aagacgatca actctattat gttggctaaa
    32221 ttaattttaa atgaatatac agcattatct gaagataaaa aagaaacatt gacaaatatt
    32281 gttcttttct gtgaacatta taaccgtcta tcagaagaaa aaattttact cccagaaaac
    32341 tgggcagaat taagaaagtt ggtagagtat gggattattt aacacacttt ccaagtcaaa
    32401 attagttaag tccagtttct ggtatactat agggaatttt tttattaagg gaatttcatt
    32461 cataactatt cctatttttg taaatattac tactcaagca gaatatggtt tgattaataa
    32521 tttcacttca tttgtatcaa ttttttcgat tcttgttggg ctaagcctaa attcggcaat
    32581 taataatgca agttttgact ataaagatga cataaatgaa tttatgtcat cggtactttc
    32641 tctagctaca ctttcgttaa ttgtgttctt attaataggg aacatatatt ttttgttttc
    32701 aaatgctttt tttgatatga accaacttgt atttaatttg atgttattac aaagttttgc
    32761 tagttttgta attagttata tccagtctta ttttacgatt aactttaacc attttaaata
    32821 tttgatttta tcttttatat ctactttttt gaatattgca atctctgtag ctttgatgca
    32881 gacattatat tttgatgata aatatgttgg cagagtagtc ggcggaacac tgtcatttgt
    32941 gttgataggt ttagtaattt atttaagaat aatgataaag ggtgctcatt tttttgataa
    33001 aaaacagtgg agatacgctt taaaaatctc tttgccgcta atcccacatt ccttagcaaa
    33061 tatagtttta gcacaattcg atagaattat gattaattct tatttaggtc ctgctgcggg
    33121 gggaatatat tcctacatta gtaacatagg tattattcta agcgtaatat ggatgtctac
    33181 taataacgcc tgggttccat ggttctatgg tgagatgagt aaaaataatg aaaaaaaagt
    33241 gaagaaagtt agtaactatt atttagtagt ctttactttg attacgatga ttgtaatggt
    33301 agtttcgatt gatttagcaa aatttatggc tccgaaagac tattattctg ggttatcttt
    33361 agtggttccg ataaccttag gctatttctt tcagttttta tatagtttac cagttaatgc
    33421 tgaattttat gagaaaaaaa catcgtttat tgcgattggt acagtagcaa gtgctatagt
    33481 taatatacta ttaaacatga ttttcatacc taggttagga ataattgctg ctggatacac
    33541 aaccgtattt acgtattttt tactttttat ttttcattat aatatcgcta aaaaaattac
    33601 gaataaacaa ttatttgaca caaaaaaaat atgtatcata ataatttttg tatcaatttt
    33661 ttctttgata cttcatattt taatcaatca aatgttgatt cgttacgttt tgacatcatt
    33721 attgataatt agtttagtta tatatttttt aaagattaag aatcgaatta tagatgacta
    33781 taattcatag gaggagatat aagtgtttaa aggaaaaaca atattagtaa ctggcggaac
    33841 tggttccatt ggaagtgaaa ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag
    33901 aatttatagt agaggtgaag aaaaacaatt ttatatgcaa caagagctct caaatcacac
    33961 taatttgaga tttttaattg gtgatatcag agactctcaa aggattgatt atgcatgtcg
    34021 tgatgtggat attatttttc atgctgccgc aatgaaacac gttccagcaa gtgaatataa
    34081 tcctatggaa gctgtaaaaa caaatgtgat gggaactcaa aatgtaatag atgcagcgat
    34141 caaaaataat gtaaaacatt ttgtaggtat cagtacagat aaagtagtga gtcctactaa
    34201 caccatgggt gcgacaaagt tattgagtga aaaattaata atagctgcca atcagtataa
    34261 aggaaaaaca gaaacaatat tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg
    34321 atctgttatt cctcttttta gtaaacaaat aaaaaacaat aaagatatta ctttgacaga
    34381 tgttgatatg acacgattta tgatgtcaat accggatgct gcaagattag ttctgtctgc
    34441 tgctatgaat tcaaaatttg gagaaacata tgtcttaaaa atgccggcaa tcaatatttt
    34501 aactttagct caatcattaa taaaaatata taatgataca catgatgaaa aatatagagg
    34561 tgaaataaaa gttgtaggta tcaggccagg agaaaaatta tatgaagagc taatgactac
    34621 agacgaaatt gaacgctcat atgaaaatga taaaatgtat atcattccac cacactatca
    34681 tgctactgac gaactgtaca aagattatga aaaaattaaa atcactgcat actcttcaga
    34741 tgcaacagaa ttaatgagtg gacaggaatt tattagaatc gcaaaagaga ataatgtcgt
    34801 gacttggtaa ggagttttat gatgaaatca gaagtatatg taattgcaga aattggtgta
    34861 aaccataatg gaagtctaga tttagcttta gaaagtattg ataaagctaa agaatgtgga
    34921 gctaacgcgg ttaaatttca aacatttaaa acgaataagc tagtttccaa aaaggctcca
    34981 atggcaaaat atcagaagga taatcttaaa gaggacagtt ctcaatttga aatgctaaaa
    35041 aaattagaat tatctcaatc tgattttatt agaataaaag attattgtat acaaaataaa
    35101 attgattttt tgtctactcc ttttgatgaa gaaagtgcag actttctaaa acaaattggt
    35161 gtacaaggat ttaaaattgg ctcaggagat ttaacaaatt taccttttct tagaaaaata
    35221 gatatgtatg ggttgccagt tttattatct acaggtatgg ctgatattca tgaggtcagt
    35281 gaggctgtag attgtttcat agatagtcca gttactattt tacattgtac ttcaaattat
    35341 ccggctagtt ctgaagatat taatttattg gcgattaaca cattaaaaga aatgttcggg
    35401 atacccgttg ggtactccga tcattcattg ggatatgatg ttggtatttg tgctgttgcg
    35461 atgggtgcta aggtaattga gaaacatttt accttggata aggaactacc tggtccagat
    35521 cataaagctt ctttagatcc tcaggaattt gcagattttg taaagcatat tagaaacact
    35581 gaaatatttt taggtgatgg tgttaagcga gcaatgcctt ctgaacttag tacaaaagaa
    35641 gtatcaagaa aaagcctagt ggtcactgag aatttaaatg tgggagatat tcttacagag
    35701 aaatctctta caataaaacg tcctggaaat ggaatagctc ctaagttttt atatcaatat
    35761 attggcaaaa aagttaaaag accaataagt gcagatagtg ttctgcttga ggaagatgtt
    35821 gaatgaaaaa aatacttata gtaagtggaa cacgagctga ttatggaata tataaacctg
    35881 ttgctgagaa tttactacaa gataattggg atgtatcatt tgttgtttct ggaatgcatt
    35941 tatcccataa atatggattt actaaagaat tgatcttagc tgacggattt agaattagtg
    36001 gagaagttca aagtttgttt ttagagaaca ataagggaaa catggctcgg tctgtggcat
    36061 tagagataaa tggtttcaca aatatatttg aacaaactag ccctgatttt gtattattac
    36121 taggggatag aggagaaatg ttggcagctg cgatagcttg tttgtatctt ggaatcaaaa
    36181 cagcacatat tcacggaggc gaagtgtctg gtactgtaga tgaatcaata agacatgcca
    36241 taacgaaaat agcatctgtt cattttgctg ctacggaaaa aagcaaagag cgtttaataa
    36301 aaatggggga agattcttgg agagtttttc ccgtcggagc tcctagaata gatactattt
    36361 taaataatag attacctagt tttagagata taaaagaaaa atatcatcta aaatttgaaa
    36421 agaaaaaata cgttttgcaa gtatttcatc cagttgtaac cgaactagaa aatatagaaa
    36481 aacaaatttt agctttgatc aattcaatgg aacaacaatc tcaaccaatt atttgcattt
    36541 tacctaatag tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa tatttaaaaa
    36601 cggatattat ttatattacg aatttagagc cggaggaata cctgactata ttaaaagagt
    36661 gcaaatttat gatcggaaat tcttcttctg ggataattga agctgcatca ttcaaaatac
    36721 cagtaataaa tttaggaacc agacaaaatg gacgagaaca atctaaaaat attataaatg
    36781 caaatctaaa tgaaaatgaa attgaaactg ctttgaataa aatacaagat cctcagttta
    36841 taaaaagctt gaaaaatgtt aagaacatct acggtgacgg caacagttct agaagaataa
    36901 caaaaacatt gaatcaaata ttgaatgatg gggaagatat taagtggatt caaaaacaaa
    36961 tagcttatta aaatatacta gtgatccata cattattatt ggggctggcg gacacgcaaa
    37021 agtcattatt gaaaccttaa gattagaaaa taagttaata tatggatttt ttgatgataa
    37081 gcaaaataaa atgcaagatc tgaaatatct tgggaaaata aacgaattga atagctgggc
    37141 ggatagcaaa aaaaaaatct ataaatttat tgtagctatt ggtgactgtg tagtaagaaa
    37201 aaagattata aaaaaattga atatgccaag tgagcaatat ggaatagctg ttcatccaag
    37261 tgctatagtg gcaaaagaaa tttcaattgg ttatggaact gttatttttg cgaatgctgt
    37321 aattaaccca gatgctgtaa ttggagagca tgctataata aacacaggta gcattgttga
    37381 gcatgattgt agaattaata actatgttca tttatctcca ggtgtatgct tagcgggtgg
    37441 agtacatgta ggtgtaggaa ctcaaatagg aattggttct caatgtatcc agatgaagaa
    37501 aattggatct tggtgtatga taggcgcagg gagcactatt gttaaggata ttccctcaca
    37561 ttccttagct tatggcaatc cagctaaaat taagaaagaa ggaattaatt ttgaattcta
    37621 gaattttatt atcaagtcct catatgggag gaacagaaga aaaatatgtt gctaaggctt
    37681 ttaaagataa ttggatagcg ccattaggtg aaaatgtgga taaatttgaa gaaagtatta
    37741 caaaatatac tggttctaag ggcggagctt ctgcgttaag ttcaggtaca gctgccatac
    37801 atcttgcctt aagactgctt aatgttggaa aaaacgatat tgtctttgct ccttcattta
    37861 cttttatagc tacagtaaat cctattttat atcaaggggc tgtaccagtg ttcattgact
    37921 cagaacctaa tacatggaat atgagccctg ttgcactaga gagcgcatta gaagaagctg
    37981 aaagaaacaa ccaattacct aaggcaatca tcgtagtgaa tttatatggt caaatggctg
    38041 attatgaacg aattttagct attgcaaacc aatttaatgt tccagtgata gaagatgcag
    38101 cggaatcgtt aggttctact tataagggaa tttcgtcagg cttatttggg acaattggga
    38161 tctattcatt taacggtaat aagatcatca caacctcagg tggaggagct ctagtgtcta
    38221 gtgataaaaa atatgttgag cattcaagat ttttagcaac acaagcaaga gatcaagcac
    38281 cgtattatca acatagcgaa ataggttata attaccgtct aagcaatatt tctgccggta
    38341 tcggacgtgg acaaatggaa gtattgaatg atagagtagc taaacgaaga gaaattttca
    38401 atcgatataa ggaagctttt tctggaattg atagaattga aatgatgcca gaagtggatg
    38461 gcatggtgcc aaatcgttgg ttgtctactt taacgatcaa tgattataat tctattgatc
    38521 aagtagttaa cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga gctttgtgga
    38581 aaccactaca tttgcaacca gtatttcaag gggtaaaaat gtattaccat gataataatc
    38641 ttagtgtgtc agaaaagtta tttaatagcg gcttatgctt accttctgga agtaatatga
    38701 cagatagtga acaagaattt gttattgatg agtttaaaaa aatattcaag taggtgtgat
    38761 attttatgaa cataggaatt attggtttag gttccatggg gaaaagaaga cttagactca
    38821 ttcaagaaca ctttaatgat attttactat gtggtgttga tactaatgag gcgagacgaa
    38881 aggaagttga aaaggaattt ggaataccaa cttataatag tctaacagag gcaaaaaatt
    38941 tgtttgaaat aaattcagtt tttgtttgta catcccctat aacccatgaa tttattataa
    39001 aagaggcttt aaatttagat tgtaatatct ttacggaaat caatttatta aatgaatatt
    39061 atgatgaaat aatcgaagta gctaaaaaga aagatagatt actttatcta tcttctacat
    39121 ttttgaagag aaaagaaatt caatatatta agtctaaagt gacgcataat aaaaaaattt
    39181 cttatcaata ccatgtagga cagtatttac ccgactggca tccttgggaa gattatcgca
    39241 atttttttgt ttcaaacaaa aaaactaatg ggtgtagaga aatattcgct attgaattac
    39301 cttggattat agatacattt gggaaagtta aatcgtttac tgtaatgacc cagaaattat
    39361 ccaatcttga tattgattat tcggacagtt tcactcttat tattgagcat gaaactggta
    39421 tagttggaac tttaagtgta gatgttgttt caagagtagc taaaagaaat ttaactataa
    39481 ttggtgaaaa gattttaata gaatgggaag gtaacccgga ttctctattt tcatggaatg
    39541 aagaaacttc tagtatgaca gatattcaat tgtacgatga ggttatgcat gataataatt
    39601 attcacatac tattattgaa gatgcatatt tagaggagat taaagaattt ttcacgcttt
    39661 tggaaaagaa aatggaaccc caatatactt ttgaaaaaga taagtatatt attaacttaa
    39721 ttaacgacat tgaggtaaat taatgatgaa agtaattttt tttgggttag ggtcaattgg
    39781 aaagagacat ctaaaaaatc taataagcta tggaaaaaag aattctatta attttgatat
    39841 aacagcttat agtagcagtg gaaaaaaaga gtttgaagaa ataacgtata ttcaaaatag
    39901 agacgatttg gatacacatt atgatatagc ttttattaca aatccgactt ttaagcatct
    39961 ggaaacacta aatgtaattg ttgaaaaagt tgattacata tttttagaaa aacctgcttt
    40021 ttctgagagt attgatctta ctcaatataa ttatgattac aaaaatattt atgttgctgc
    40081 tccactgagg tataaaaata tcatggaagt tgttcgtaat agtattcaaa gtaaaaaagt
    40141 atattctgta agagctattt gttctactta tcttcctgat tggagaaaag gtactgatta
    40201 tagaaataat tattctgcct ttaaatctat ggggggagga gtagaattag actgtataca
    40261 tgaattggat tatattataa acctatttgg tttccctaaa tcagtaaaaa aacagtttgg
    40321 taaatattcg aatcttgaaa ttaacagtaa tgatttagca acatatatat tgagatacga
    40381 tgataaaatt atagaaattc atttagatta ttttggaaga gttcctacaa gaaaatttca
    40441 attgtttact ttagatgaat gtatcaatgt tgacttatta aataataaaa taagttttga
    40501 agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat atgtatgaga aggagttgaa
    40561 atatttcttt gaaaacgtag taacaggcaa agaaaactgg aatgatttat atcatgcaaa
    40621 cgaagtattg aaaatagctg aggagaaata aatggaaatt ttatttacta tttgtggtag
    40681 agctgggtct aaggggttta aaaataaaaa tttgaaatat ttggcaggaa agcccctagt
    40741 ccatttcgct ttagcaatga tagatatcta tcaacaaaaa catcctgaac ataattgcac
    40801 gacttgtttg aatacggaca gtaatgagtt agtagaattg acgactaagt taaatccgtc
    40861 tgtgaaaatt gtagaacgta aacctgaatt agctacagat actgtaggga aaattgaagt
    40921 gatacgtgat tcattcttac aactagaaag agaagataat atatttgatt taattataga
    40981 tttagatttg acttcacctt tacgtgaagt ttctaatctt gaagaattaa tagatgaaca
    41041 tctaaaaaat ttagataaag acgtctactt ttcagttgtt tctgcaagac gtaatcccta
    41101 ttttaatatg gtaaaaagaa ttgaaaatga agttactata gttaataaat caagatacac
    41161 atctaggcaa cagacacctg aagtttttga aatgaatgcc tcaatgtatt cttttaaacc
    41221 tgaatttcta aaaaaaagta attatatttt tgatgggcaa tgtgggataa taaaaatgag
    41281 agattatcta attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt
    41341 tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag
    41401 gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag
    41461 ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag
    41521 tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat
    41581 ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag
    41641 ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa
    41701 gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt
    41761 ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg
    41821 tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag
    41881 attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc
    41941 caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata
    42001 agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta
    42061 gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag
    42121 ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga
    42181 ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt
    42241 cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac
    42301 cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca
    42361 tactaagata agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt
    42421 gtaaaatcaa atctgggata gaagggctct ttgtcaaatg atgttggtaa agagaaatct
    42481 tagaataatt tggacagaac agaaagaaga aaacctcttt ctgttctgtt tatttggtta
    42541 aaaatgtttt gccatgacga atcataattc gtagtttgaa
//
LOCUS       DASHFF010000019        39680 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.019, whole genome shotgun sequence.
ACCESSION   DASHFF010000019 DASHFF010000000
VERSION     DASHFF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.019"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(148..2301)
                     /locus_tag="U2I75_001147"
     CDS             complement(148..2301)
                     /locus_tag="U2I75_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN1696294.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="U2I75_001148"
     CDS             complement(2595..3443)
                     /locus_tag="U2I75_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1696295.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="U2I75_001149"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="U2I75_001149"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN1696296.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="U2I75_001150"
     CDS             complement(5944..6123)
                     /locus_tag="U2I75_001150"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN1696297.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="U2I75_001151"
     CDS             complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="U2I75_001151"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1696298.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /locus_tag="U2I75_001152"
     CDS             8271..10550
                     /locus_tag="U2I75_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1696299.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="U2I75_001153"
     CDS             complement(10643..11290)
                     /locus_tag="U2I75_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696300.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="U2I75_001154"
     CDS             complement(11469..12188)
                     /locus_tag="U2I75_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1696301.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="U2I75_001155"
     CDS             complement(12319..13470)
                     /locus_tag="U2I75_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN1696302.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="U2I75_001156"
     CDS             complement(13687..14427)
                     /locus_tag="U2I75_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1696303.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="U2I75_001157"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="U2I75_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN1696304.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="U2I75_001158"
     CDS             complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="U2I75_001158"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN1696305.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16038)
                     /locus_tag="U2I75_001159"
     CDS             complement(15388..16038)
                     /locus_tag="U2I75_001159"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN1696306.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="U2I75_001160"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="U2I75_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN1696307.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="U2I75_001161"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="U2I75_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN1696308.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="U2I75_001162"
     CDS             complement(17481..18008)
                     /locus_tag="U2I75_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN1696309.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="U2I75_001163"
     CDS             complement(18188..18973)
                     /locus_tag="U2I75_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN1696310.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /locus_tag="U2I75_001164"
     CDS             complement(18966..19835)
                     /locus_tag="U2I75_001164"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN1696311.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="U2I75_001165"
     CDS             complement(19836..21215)
                     /locus_tag="U2I75_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN1696312.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="U2I75_001166"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="U2I75_001166"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1696313.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="U2I75_001167"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="U2I75_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN1696314.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="U2I75_001168"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="U2I75_001168"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN1696315.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGTKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="U2I75_001169"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="U2I75_001169"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN1696316.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="U2I75_001170"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="U2I75_001170"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1696317.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="U2I75_001171"
     CDS             complement(25173..25397)
                     /locus_tag="U2I75_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1696318.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="U2I75_001172"
     CDS             complement(25450..26412)
                     /locus_tag="U2I75_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN1696319.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="U2I75_001173"
     CDS             complement(26422..26862)
                     /locus_tag="U2I75_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1696320.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILHGMATEEQHALLDALKNLHDFLQDYK"
     gene            27168..28133
                     /locus_tag="U2I75_001174"
     CDS             27168..28133
                     /locus_tag="U2I75_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1696321.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28190..28420)
                     /locus_tag="U2I75_001175"
     CDS             complement(28190..28420)
                     /locus_tag="U2I75_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN1696322.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28506..29393)
                     /locus_tag="U2I75_001176"
     CDS             complement(28506..29393)
                     /locus_tag="U2I75_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN1696323.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29482..30705)
                     /locus_tag="U2I75_001177"
     CDS             complement(29482..30705)
                     /locus_tag="U2I75_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1696324.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30722..31768)
                     /locus_tag="U2I75_001178"
     CDS             complement(30722..31768)
                     /locus_tag="U2I75_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1696325.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="U2I75_001179"
     CDS             complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="U2I75_001179"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN1696326.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33659..33925)
                     /locus_tag="U2I75_001180"
     CDS             complement(33659..33925)
                     /locus_tag="U2I75_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN1696327.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34404..36638
                     /locus_tag="U2I75_001181"
     CDS             34404..36638
                     /locus_tag="U2I75_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1696328.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36786..37106
                     /locus_tag="U2I75_001182"
     CDS             36786..37106
                     /locus_tag="U2I75_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN1696329.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37311..37535)
                     /locus_tag="U2I75_001183"
     CDS             complement(37311..37535)
                     /locus_tag="U2I75_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEN1696330.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37733..39313)
                     /locus_tag="U2I75_001184"
     CDS             complement(37733..39313)
                     /locus_tag="U2I75_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN1696331.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
BASE COUNT        11136 a         8567 c         6889 g        13088 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcagatatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgta cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggtagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tgtaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    27001 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    27061 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    27121 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    27181 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    27241 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    27301 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    27361 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    27421 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    27481 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    27541 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    27601 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    27661 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    27721 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    27781 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    27841 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    27901 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    27961 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    28021 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    28081 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    28141 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    28201 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    28261 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    28321 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    28381 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    28441 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    28501 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    28561 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    28621 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    28681 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    28741 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    28801 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    28861 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    28921 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    28981 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    29041 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    29101 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    29161 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    29221 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    29281 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    29341 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    29401 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    29461 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    29521 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    29581 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    29641 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    29701 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    29761 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    29821 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    29881 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    29941 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    30001 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    30061 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    30121 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    30181 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    30241 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    30301 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    30361 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    30421 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    30481 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    30541 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    30601 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    30661 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    30721 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    30781 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    30841 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    30901 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    30961 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    31021 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    31081 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    31141 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    31201 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa
    31261 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    31321 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    31381 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    31441 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    31501 ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    31561 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    31621 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    31681 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    31741 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    31801 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    31861 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    31921 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcatatcg
    31981 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    32041 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    32101 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    32161 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    32221 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    32281 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    32341 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    32401 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    32461 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    32521 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    32581 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    32641 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    32701 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    32761 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    32821 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    32881 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    32941 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    33001 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    33061 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    33121 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    33181 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    33241 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    33301 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    33361 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    33421 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    33481 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    33541 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    33601 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    33661 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    33721 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    33781 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    33841 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    33901 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    33961 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    34021 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    34081 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    34141 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    34201 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    34261 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    34321 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    34381 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    34441 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    34501 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    34561 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    34621 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    34681 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    34741 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    34801 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    34861 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    34921 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    34981 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    35041 atgtgatcct attcatagat gaagttcacg aaatcgtcgg tgctggttct gctggtgatg
    35101 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    35161 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    35221 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    35281 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    35341 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    35401 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    35461 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    35521 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    35581 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    35641 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    35701 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    35761 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    35821 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    35881 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    35941 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    36001 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    36061 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    36121 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    36181 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    36241 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    36301 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    36361 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    36421 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    36481 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    36541 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    36601 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    36661 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    36721 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    36781 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    36841 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    36901 ctgaagcccc tgaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    36961 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    37021 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    37081 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    37141 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc gaaagcagaa
    37201 gcaactcctt cggaaataag ctgaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    37261 ccagcttatt tcttggagtt aaacgcttct gtctcagcct ctttttcttt ctagcttttt
    37321 gatttttctc ttttcttttg tacttttctc ttttcatttt cagttctacg cgtgtcttta
    37381 tttttaactt tttcctcttt atggatgatt gatcgtactt tttgtgcaat ttgctcgttt
    37441 ttgtcccagt cttttacatc taataatcga cataatcgta acatctcttc ttttttctgt
    37501 ttttttcttt cacgtctttg ccattctgtt tccatgtgtc acactctcct ttctttaatc
    37561 atatgtacgc aaaataattt ttatattttt ttgattagac atttctatga ttttaatata
    37621 gtaaaaagag ccgtcacgga aaatccgtca cggctctaaa caaaatatga gaggctgttt
    37681 ttgtttggct attcttatta cttgatgcag ggcagcttgt gtcaagactt ctttacatca
    37741 aactttttaa ttttacattg ggatatttat ccgcaaacca gcgttctgca aattggttct
    37801 cgaataagaa caacggttgg tcgaaacggt cacgagccaa gatattgcga cttgaactca
    37861 tcttctcatc caaatcttct ggatcgatcc agcgagcgat tttgtgtccc attggagaca
    37921 tgattacttc ggcgttatac tcatttaaca tacgatattg gaatacttcg aattgtagct
    37981 gaccaacagc tccaatgata tattcttccg ttagatatgt tttataaagc tgaatcgctc
    38041 cttcttgaac taattgatag attcctttgt ggaaggattt ttgtttcatg acattttttg
    38101 cagtcacttt cataaacagc tctggagtaa atgaagggag ttcttcataa gcaactttta
    38161 gttttccttc gaacaatgta tccccaatct gataattgcc tgtatcatat acaccgatga
    38221 tatcgcctgc tactgctttt tcgacgtttt ctctcgaatc tgccataaat tgtgtgacat
    38281 tgcttaattt cagtttttta tttgtacgtt ccaaccagac atccattcct cgttcgaatg
    38341 ttccagaaca gattcggaca aaagcgattc ggtcacgatg ggctggattc atatttgctt
    38401 ggatcttgaa tacgaaacct gaaaattctt cctcataagg acttaccatc tgatcatctt
    38461 gtgttttgtg tccataagga gctggtgcca attcaacgaa agtctctaaa aaagtctgta
    38521 caccgaagtt agtcaatgca gaaccaaaaa atactggtgt ctgatcgcca cgagcaattt
    38581 tttcttgatt aaatgcatct ccagcctctt tgattagttc tacttcttct aagacttgtt
    38641 catataagga attttcagtt agaggatgat tttcgggaag cagtccatct tcatccaatt
    38701 ctactaatcg ttcatcctga taattttccg gacgataaaa ctctacacga ttatggtaga
    38761 tatcataaag accttctagc cctttaccca taccgattgg ccaattcatc ggataagatt
    38821 cgatatctaa tagctcttct aattcttcta acaattccaa tggttcacga ccatcacgat
    38881 ctagtttgtt gataaaagta aatatcggga tgccacgttt ttttacaact tggaataatt
    38941 tctttgtctg tgcttcgatc ccttttgcac tatcgattac catgaccgca ctatccacgg
    39001 ccatcaaagt acgataagta tcttcagaaa aatcttcatg tcctggtgta tccaagatat
    39061 ttactcgttt cccttgataa tcaaactgca taacagaact agtaacagaa atcccacgtt
    39121 gtttctcgat ttccatccag tctgatttgg caaaattacc tgtttttttg ccttttacgg
    39181 tcccggcttg acggatagcc ccaccaaaca atagtaattg ttctgtaatc gttgttttcc
    39241 cagcatccgg atgggaaatg atcgcaaaag ttctgcgatt gtccacttgt tgctttaatt
    39301 ttggattatt cataacctaa ctccatttct tccattcaat tatttatatt ttctctaggc
    39361 acagattgtc cgatctctta aagaaagaca atttcatata gggctgtgac aaaagccgtg
    39421 tagcagtctc tttttccgag taaacggcgt tctagagaca gcctatcagc aataagccca
    39481 cccaagccaa aaatacgaaa agctgttttt gcttggctgc tcttattact taatgcagga
    39541 tagctttttc acaacctttg tcaactacta caactgaatc agtatagcat ggattcagcg
    39601 aatgaaaaag ccaccaaacc ataagagtca aatatttttt tgtcagctga ctaaaagaaa
    39661 agacgtgttg cttgtttatt
//
LOCUS       DASHFF010000020        39583 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.020, whole genome shotgun sequence.
ACCESSION   DASHFF010000020 DASHFF010000000
VERSION     DASHFF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.020"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(448..2355)
                     /locus_tag="U2I75_001185"
     CDS             complement(448..2355)
                     /locus_tag="U2I75_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN1696332.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2485..3360
                     /locus_tag="U2I75_001186"
     CDS             2485..3360
                     /locus_tag="U2I75_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN1696333.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3440..4414
                     /locus_tag="U2I75_001187"
     CDS             3440..4414
                     /locus_tag="U2I75_001187"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1696334.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4594..5739
                     /locus_tag="U2I75_001188"
     CDS             4594..5739
                     /locus_tag="U2I75_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1696335.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5876..6220
                     /locus_tag="U2I75_001189"
     CDS             5876..6220
                     /locus_tag="U2I75_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN1696336.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6491..7777
                     /gene="efmA"
                     /locus_tag="U2I75_001190"
     CDS             6491..7777
                     /gene="efmA"
                     /locus_tag="U2I75_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN1696337.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7915..8514)
                     /locus_tag="U2I75_001191"
     CDS             complement(7915..8514)
                     /locus_tag="U2I75_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN1696338.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8918..10039
                     /gene="mnmA"
                     /locus_tag="U2I75_001192"
     CDS             8918..10039
                     /gene="mnmA"
                     /locus_tag="U2I75_001192"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN1696339.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            10285..11706
                     /gene="arcD"
                     /locus_tag="U2I75_001193"
     CDS             10285..11706
                     /gene="arcD"
                     /locus_tag="U2I75_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN1696340.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11720..12838
                     /locus_tag="U2I75_001194"
     CDS             11720..12838
                     /locus_tag="U2I75_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN1696341.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12898..14190)
                     /locus_tag="U2I75_001195"
     CDS             complement(12898..14190)
                     /locus_tag="U2I75_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1696342.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14322..14900
                     /locus_tag="U2I75_001196"
     CDS             14322..14900
                     /locus_tag="U2I75_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1696343.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            15029..15406
                     /locus_tag="U2I75_001197"
     CDS             15029..15406
                     /locus_tag="U2I75_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN1696344.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15339..17411
                     /locus_tag="U2I75_001198"
     CDS             15339..17411
                     /locus_tag="U2I75_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1696345.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17622..18569)
                     /locus_tag="U2I75_001199"
     CDS             complement(17622..18569)
                     /locus_tag="U2I75_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1696346.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            18815..19279
                     /locus_tag="U2I75_001200"
     CDS             18815..19279
                     /locus_tag="U2I75_001200"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN1696347.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(19341..20384)
                     /locus_tag="U2I75_001201"
     CDS             complement(19341..20384)
                     /locus_tag="U2I75_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN1696348.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(20761..21726)
                     /locus_tag="U2I75_001202"
     CDS             complement(20761..21726)
                     /locus_tag="U2I75_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN1696349.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            21960..22805
                     /locus_tag="U2I75_001203"
     CDS             21960..22805
                     /locus_tag="U2I75_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1696350.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            22996..24183
                     /locus_tag="U2I75_001204"
     CDS             22996..24183
                     /locus_tag="U2I75_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1696351.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            24189..24545
                     /locus_tag="U2I75_001205"
     CDS             24189..24545
                     /locus_tag="U2I75_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN1696352.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            24547..24885
                     /locus_tag="U2I75_001206"
     CDS             24547..24885
                     /locus_tag="U2I75_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN1696353.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    24961..25217
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            25280..26314
                     /locus_tag="U2I75_001207"
     CDS             25280..26314
                     /locus_tag="U2I75_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1696354.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            26307..26993
                     /locus_tag="U2I75_001208"
     CDS             26307..26993
                     /locus_tag="U2I75_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696355.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            27017..27865
                     /locus_tag="U2I75_001209"
     CDS             27017..27865
                     /locus_tag="U2I75_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1696356.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            28062..28835
                     /gene="sufC"
                     /locus_tag="U2I75_001210"
     CDS             28062..28835
                     /gene="sufC"
                     /locus_tag="U2I75_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN1696357.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            28848..30134
                     /gene="sufD"
                     /locus_tag="U2I75_001211"
     CDS             28848..30134
                     /gene="sufD"
                     /locus_tag="U2I75_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN1696358.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            30131..31366
                     /locus_tag="U2I75_001212"
     CDS             30131..31366
                     /locus_tag="U2I75_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1696359.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            31353..31823
                     /locus_tag="U2I75_001213"
     CDS             31353..31823
                     /locus_tag="U2I75_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN1696360.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            31828..33219
                     /gene="sufB"
                     /locus_tag="U2I75_001214"
     CDS             31828..33219
                     /gene="sufB"
                     /locus_tag="U2I75_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN1696361.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(33314..34456)
                     /locus_tag="U2I75_001215"
     CDS             complement(33314..34456)
                     /locus_tag="U2I75_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1696362.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(34495..35229)
                     /locus_tag="U2I75_001216"
     CDS             complement(34495..35229)
                     /locus_tag="U2I75_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1696363.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            35461..35736
                     /locus_tag="U2I75_001217"
     CDS             35461..35736
                     /locus_tag="U2I75_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1696364.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            35834..37831
                     /locus_tag="U2I75_001218"
     CDS             35834..37831
                     /locus_tag="U2I75_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696365.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(37907..38242)
                     /locus_tag="U2I75_001219"
     CDS             complement(37907..38242)
                     /locus_tag="U2I75_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1696366.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            38369..38584
                     /locus_tag="U2I75_001220"
     CDS             38369..38584
                     /locus_tag="U2I75_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1696367.1"
                     /translation="MKKVNNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            38621..38983
                     /locus_tag="U2I75_001221"
     CDS             38621..38983
                     /locus_tag="U2I75_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEN1696368.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            39029..39355
                     /locus_tag="U2I75_001222"
     CDS             39029..39355
                     /locus_tag="U2I75_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696369.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        12902 a         7059 c         8260 g        11362 t
ORIGIN      
        1 tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag
       61 gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag
      121 ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa tactcaggtc
      181 aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt ctcggtagta
      241 agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt actacttgaa
      301 actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact tcttcgaaaa
      361 ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt cttatttctt
      421 gaagctgacc acttctggca caacctctta ccagattcgc actctttttt ctggcgctag
      481 atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg
      541 ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg
      601 gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga attcctcgat
      661 agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc
      721 aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc
      781 gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca
      841 ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga
      901 aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg attgttctag
      961 actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg
     1021 ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt
     1081 ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa
     1141 agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc
     1201 ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc
     1261 atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac
     1321 catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc
     1381 aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc
     1441 ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga
     1501 tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa
     1561 atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac
     1621 tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt
     1681 gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc
     1741 ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat
     1801 tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa
     1861 gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg
     1921 aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc
     1981 atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctt
     2041 attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt gaagcggttg
     2101 agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg
     2161 tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat
     2221 caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc
     2281 agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa
     2341 tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa
     2401 aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt
     2461 gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta
     2521 gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag
     2581 cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt
     2641 tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat
     2701 caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct
     2761 agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag
     2821 gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg
     2881 gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat
     2941 caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat
     3001 tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt
     3061 ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt
     3121 gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa
     3181 cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca
     3241 gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg
     3301 ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa
     3361 gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag
     3421 aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag
     3481 tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg
     3541 taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat
     3601 tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg attactatat
     3661 ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt
     3721 attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac
     3781 tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga
     3841 tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat
     3901 gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt
     3961 ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac
     4021 attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga
     4081 tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc
     4141 acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca
     4201 tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg
     4261 tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac
     4321 atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac cgatgagtcc
     4381 gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga aagaagttgt
     4441 gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct ccttttttct
     4501 atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt cgctatttta
     4561 tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac tatttatctg
     4621 gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac ggcaatcatg
     4681 caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc gcatgaaaaa
     4741 ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca cgagatcatc
     4801 tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc gttctcgcgt
     4861 caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt tcttaatacc
     4921 atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg
     4981 aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt ggtctctgtg
     5041 atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg tgaactttta
     5101 gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa ccaaacgatc
     5161 catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat caatgggcca
     5221 aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt gcttttaggc
     5281 ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat ctggggtatg
     5341 gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa
     5401 ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta tcaagtaaat
     5461 ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat
     5521 gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg ttcagcttgt
     5581 acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga agatacaccg
     5641 atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga cgacatccag
     5701 tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc attcttatat
     5761 gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa aacctagtaa
     5821 aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga aaatcatggc
     5881 atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc atcctgatgc
     5941 aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca attatcaatt
     6001 gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa aaatcactgt
     6061 atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt
     6121 caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt ttctgatgga
     6181 tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata taaaaagaca
     6241 actagttttg aaacggccta ttgttagatg actaactttg taaggacgac gcaaattagt
     6301 tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg ctgatttatt
     6361 gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa ggtatttttt
     6421 gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga aaaagaaaag
     6481 agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga gaaattattt
     6541 gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc aatatgcgat
     6601 catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg ctactttact
     6661 aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg atcgctggga
     6721 taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc ttattttagc
     6781 agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt ctttgtttat
     6841 gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc cgacaatcgt
     6901 tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat cagcgaattt
     6961 catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca attaccttat
     7021 tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg tgaagatccc
     7081 aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg caaaatttgg
     7141 gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg gtgcatttgt
     7201 gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag attactttgg
     7261 tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa tgctcttagg
     7321 cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta ttattatagc
     7381 ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg atagtcaagg
     7441 gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat atttcaatac
     7501 actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc gtgtcttagg
     7561 gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg caggaccatt
     7621 agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga ttttattatg
     7681 cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat tacagaaaaa
     7741 actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt tgtgacagaa
     7801 gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc tactcaaatg
     7861 aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt tttttcacga
     7921 cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag ataacggagt
     7981 gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc ctacattgac
     8041 atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc ggatagtgtc
     8101 gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt cgaatttaac
     8161 gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc taggatcatt
     8221 tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca agtggattgt
     8281 tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat attgcggatg
     8341 catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg cattccagct
     8401 agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag caaagtcgtt
     8461 ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat tcatgtatct
     8521 tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact cataacatct
     8581 aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca attttgaaaa
     8641 atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc ctttttatga
     8701 gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt tgactatcat
     8761 gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa ttaagggatg
     8821 aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc ttcaactcgt
     8881 agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca gcaaaacacg
     8941 cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt tgttaaagga
     9001 acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg acgaaaacgg
     9061 tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc aaatcgggat
     9121 tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg aatatttcct
     9181 agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca aagaaatcaa
     9241 attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag ctactggaca
     9301 ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg gagtagacaa
     9361 taataaagat caaacttact tccttagcca attatcacaa gaacagcttt caaaaacgat
     9421 gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac gcgcaggcct
     9481 tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa agaacttcaa
     9541 acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc ttgatggtga
     9601 agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc aaggactagg
     9661 gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag atctagcgac
     9721 aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca caagcttgga
     9781 tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca agtgtacagc
     9841 gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg atgataatcg
     9901 tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag cagttgtatt
     9961 ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc aagaaacaaa
    10021 agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc taacaaaaga
    10081 aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat ttttattcat
    10141 tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga ataaagttgc
    10201 tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt gatttgttca
    10261 attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag caaatgggga
    10321 ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat cacaagtgat
    10381 ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt tgggattggt
    10441 attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga tctagatggt
    10501 ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat cagtggttgg
    10561 ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct gatgagcgcc
    10621 atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat tttatttgca
    10681 agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg agcgagcgtc
    10741 atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt aatatttgta
    10801 tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt atctgataat
    10861 ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg ttgtttgatg
    10921 gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa ccgcgcgaag
    10981 aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt gtttatctat
    11041 atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc aactatttca
    11101 caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc agcgctgatc
    11161 aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat gctcccagca
    11221 gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg aaaaacgaat
    11281 aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa tctattctta
    11341 ttgactttct tagttactga ttatgcctat caatttgcct attctttatg tacagcggca
    11401 attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt aaaagaagga
    11461 atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat gggcatcatt
    11521 ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg tttttgcttc
    11581 tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa agtaatgatt
    11641 gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg ctggatcaag
    11701 atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat ggctaaataa
    11761 atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat tgacattaga
    11821 cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac gcaaaaacga
    11881 accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag tcgcttctct
    11941 ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg gtgctaatct
    12001 tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc ttcctactta
    12061 tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg ttcatttgaa
    12121 ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca aacctgaaac
    12181 aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag atcggaaaac
    12241 tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga tagatgaagt
    12301 atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct atgaaaaagg
    12361 tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta ttggctggac
    12421 agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca cgatgatttg
    12481 cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag aaaaaatatt
    12541 ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat gggtagccaa
    12601 tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca ttaaattagc
    12661 catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag gagtactatt
    12721 agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact gttgtaaaaa
    12781 agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac aggcctaaac
    12841 aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca tttacgctta
    12901 ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt tttcttctgc
    12961 agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt tgaccaaaac
    13021 aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa cactggagcc
    13081 gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac tgaagatccg
    13141 tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag ggagcagaca
    13201 agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat tcgtgacgaa
    13261 tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta ttctttcact
    13321 cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg caatcccagg
    13381 tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca gcaattggcg
    13441 aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga tcatcgttgt
    13501 gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct gtttggcaga
    13561 aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca gactcaacaa
    13621 gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc caaacgctga
    13681 agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac cagttgctaa
    13741 ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa gggcagtggc
    13801 attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta attgatacac
    13861 actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag cacgcaacag
    13921 catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga taagcgtagt
    13981 caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg acgtactata
    14041 attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa atccgatacc
    14101 agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt ctcttttcct
    14161 aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa aattttcatc
    14221 tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc ttttttctct
    14281 ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga aacatggaaa
    14341 tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa ctagcagaag
    14401 agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat atcaaggctc
    14461 cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta acagcgaaac
    14521 ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt ggtcaggaag
    14581 cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa gcagtgtatg
    14641 agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga atccatgaaa
    14701 cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa gaaaaagttc
    14761 atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt tttttaggat
    14821 actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg atcaataact
    14881 ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag atgttcatta
    14941 aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact aagatgagtc
    15001 tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt tcggggacaa
    15061 tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag aaacattcga
    15121 gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc atcaaaatta
    15181 tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg gagatgagcg
    15241 ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt tgttgaaaaa
    15301 tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta attctagcgg
    15361 aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa aagcacaaaa
    15421 attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta ttaggattga
    15481 aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg ttgccaatga
    15541 tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta aaagcaatca
    15601 agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat gccggaagag
    15661 aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa ccagtgaagc
    15721 ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag caactaagac
    15781 ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa gcggactggc
    15841 tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat ttatcagcag
    15901 gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa atcgagcagt
    15961 tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa gccaaaatgc
    16021 ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt gttcaattga
    16081 tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg gaatctgcgc
    16141 cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat ggctattctg
    16201 caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc gttacttatc
    16261 cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa gaacgattgc
    16321 aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga gccatcgtcc
    16381 aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt ttgccaactg
    16441 aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat caaatgattg
    16501 tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca aaagttacgg
    16561 ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa gcaggatgga
    16621 aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg agaatcaatc
    16681 ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca gaagcaagct
    16741 tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca gagattatcg
    16801 agaaactaat caaatctgaa ctgatggaac gtacccctca aggattgcaa gtatctccta
    16861 aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat ttgacagaaa
    16921 gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga accttcttga
    16981 atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc aaacaagaat
    17041 atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat ctgcgcgaga
    17101 aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac taccgcagaa
    17161 gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa atggaaatca
    17221 tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca gaaaaattgc
    17281 ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc ttgctgaaaa
    17341 aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta aaagcagcaa
    17401 tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc aagaaataag
    17461 aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa
    17521 gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt ttgtcacagt
    17581 cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg aggcttcaat
    17641 tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg tttcgtttct
    17701 aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg gattccacca
    17761 atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc aaacaacaat
    17821 gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa agcgccgata
    17881 aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat cgagtagttg
    17941 aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt ttcataagaa
    18001 atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg gttgtattct
    18061 gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc acgagcgcca
    18121 tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt cgcaaagaaa
    18181 ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca atcgatatca
    18241 gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg cagatcaaca
    18301 tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag agccgcttct
    18361 ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga ggctagcttc
    18421 ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata ttcgaactca
    18481 tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt ttcttcatta
    18541 aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt atgtttatat
    18601 atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt gattttacgt
    18661 aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt tcctcggttt
    18721 atcaaacttt taagaacaat ttttattagt tggcaagtag ttttggatag agaaaaagga
    18781 ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac ctatcggctt
    18841 tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc caatttcaga
    18901 aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg aagaatattt
    18961 tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta cgccttttca
    19021 gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa cgtataaaga
    19081 agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg caaatcgaac
    19141 caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg gaaaactgac
    19201 aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt tgttgttaga
    19261 aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc gagaatcaca
    19321 actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa agccatcact
    19381 gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc tttcatgaat
    19441 tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa ccagctgctg
    19501 ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt gaccaaacaa
    19561 aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt ctgcgtatca
    19621 gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa cagatagaca
    19681 acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc gacaacagaa
    19741 ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt accacccaaa
    19801 agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa agggagagtc
    19861 aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt ttcttcctct
    19921 ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac agcgtttcct
    19981 ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat caagtaagca
    20041 ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac accaatttga
    20101 gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc taatagtttg
    20161 ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt atttcctaat
    20221 aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata aatagcaaga
    20281 agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat ttcgcggacc
    20341 gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc ggttcctttc
    20401 gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat tttgacaaaa
    20461 gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa cggtgttcca
    20521 gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta
    20581 aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt aaacggtgtt
    20641 caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac agctgttttt
    20701 gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt gaatcgatta
    20761 ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa ttaagaaacg
    20821 tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt ccgcaacttt
    20881 taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa cagtagtaaa
    20941 tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg gataaaaacg
    21001 aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt gagataaagc
    21061 gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat aacctgttag
    21121 gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat gagccatatt
    21181 tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc agatcaatga
    21241 aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta cagtgatgac
    21301 cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg aaagatcagc
    21361 tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga taacagcgca
    21421 cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat aagtacaaat
    21481 aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat catgcaacgt
    21541 ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca taagaatcat
    21601 agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa acagatttcc
    21661 aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt tcaaaaagtt
    21721 cttcatatat aatattctcc tttttattta atagatttta gtaccaagat gaacgaatac
    21781 attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt gtcttcatct
    21841 aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga tggttttttg
    21901 tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga agtggtgcca
    21961 tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt tccagagcag
    22021 cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag ttggagcggg
    22081 aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca actaagcaag
    22141 ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa actcagcaaa
    22201 aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc cacacctttg
    22261 ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct aaagtgcatt
    22321 ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa catgaatccg
    22381 attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac tctttgatgg
    22441 aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta agagaagcaa
    22501 actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc caagataaga
    22561 agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga atcggtcgct
    22621 cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg aatttccatc
    22681 gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta gagattgccg
    22741 aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag aatctcagct
    22801 ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa gcagtaggct
    22861 tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc ccaaatgtct
    22921 atggaaagac tttccattat ctaactggac cgctataata atcagcagta tggtttttaa
    22981 gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg tattgattat
    23041 ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt atatgtagca
    23101 cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca agttgtctgg
    23161 tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa atggatctgg
    23221 aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt gctcaaattc
    23281 tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt cggtgcgttt
    23341 actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc actgatcgta
    23401 actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg gctgattggg
    23461 aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga tgactttgga
    23521 acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg aatctcttgg
    23581 cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac gatcttcttg
    23641 gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc ttatcagttt
    23701 gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag ttatcagccg
    23761 gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt caacgtaagc
    23821 aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg tgaaaacttc
    23881 ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta tcgtatgatt
    23941 cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg tttgatcatg
    24001 atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact tcctttaaca
    24061 gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa catgatcggt
    24121 atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc ttcaaggagg
    24181 taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa aaagccaaag
    24241 cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa gaagtaccga
    24301 aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt cgcctgttca
    24361 acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc gagtttacgg
    24421 cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga ccactgatct
    24481 tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa ggagaactga
    24541 tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt atttaatgga
    24601 aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga catcacgttt
    24661 gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat cgaagttgaa
    24721 gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc ttctgttaat
    24781 gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa tgcgtggatc
    24841 ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca gaaaatcact
    24901 tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc tatatgaaac
    24961 gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc ctgtttgctg
    25021 aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc gagcgtaagc
    25081 aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag ctaagtccaa
    25141 gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac acggaagttc
    25201 aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg aaaaatcata
    25261 agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa agacattcaa
    25321 tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg aaaaagggga
    25381 tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc gtctcctgaa
    25441 tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata ttacaaaatt
    25501 aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc agcatttcaa
    25561 cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat tagctggcgt
    25621 accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg ggttagaagg
    25681 acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg tcggtatcgc
    25741 acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta gcgcattgga
    25801 cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga aattgaattt
    25861 gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca acaaagtcgc
    25921 tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct tcagacaacc
    25981 gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag ctgattcgac
    26041 agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa ctttgacctt
    26101 tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct ataacatcga
    26161 tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg gttcattgac
    26221 tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca tcaaagaaca
    26281 aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca tatctagatt
    26341 ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta tatatgacat
    26401 taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc ctttactcta
    26461 ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg atcatcagta
    26521 atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca tttactaaag
    26581 caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt ttatccgcag
    26641 caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct ggtgtactgg
    26701 aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc ttgatacctg
    26761 aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg atcggattta
    26821 ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg caggaaggct
    26881 accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta ctaatcgtat
    26941 ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga taataggata
    27001 tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc ttttgctgac
    27061 tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa ataaagcaga
    27121 cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt tggaacatgt
    27181 gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg atgactatgt
    27241 attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc agcatattcc
    27301 ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg gtgctgtcca
    27361 catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat taaaagatgg
    27421 tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca caattctaca
    27481 agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa cattcgatga
    27541 tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat cacttttagc
    27601 ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact tcgcttacgg
    27661 cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact ctccttacgt
    27721 gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa aactgattga
    27781 agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg gctctgttaa
    27841 accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa taagtctgta
    27901 atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa ttgtttaata
    27961 ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc tcaattagtc
    28021 taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc ttagaaataa
    28081 aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac ctaacaatga
    28141 aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct accttatctg
    28201 ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg tttgacggga
    28261 aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt ttagctatgc
    28321 aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca gcgatcaatg
    28381 cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag ttagatgaaa
    28441 aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac gaaggttttt
    28501 ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa cctacatttg
    28561 caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc gttgctaaag
    28621 gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac tatcaacgat
    28681 tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga gtagtcatga
    28741 cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt atcagtaaag
    28801 aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg aaagaaaaaa
    28861 actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc gaacctgatt
    28921 ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca ttgccgcata
    28981 tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca caaaccatca
    29041 gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg cttgtccaac
    29101 aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa ggagtcattt
    29161 ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa tattatatga
    29221 aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct tttatgaaca
    29281 gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc gagtccttat
    29341 tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc gtggcagatg
    29401 aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa gtagcaaaat
    29461 catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc aaatattcag
    29521 ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg cacattttac
    29581 gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg atcgctgact
    29641 ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg gcaatcagct
    29701 cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct catacgatcg
    29761 ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt aatggtatcg
    29821 gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt gtcttgatgc
    29881 tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa aacgaagtaa
    29941 ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac tatctgatga
    30001 gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc ctcggttctg
    30061 tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta atcgaaggga
    30121 agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct tatttcaaga
    30181 cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa agccaaaaca
    30241 agtactccag gcattagcgt actattacga acacgataac gccaatgttc atcgcggtgt
    30301 ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa aagtacgggc
    30361 gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga cgacaagctt
    30421 gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg agatagtgct
    30481 ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa aacgaacagg
    30541 tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg aggatgcagc
    30601 gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca atgtcttagg
    30661 tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg gggtcatcgt
    30721 agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac tagactgtga
    30781 tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg ttttatatgg
    30841 taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa tgatcgattt
    30901 tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag cagggacacc
    30961 aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa aactcggtat
    31021 ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa aattacaagc
    31081 aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag gagtcgttgc
    31141 attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca tggaaggggt
    31201 agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg aagtatcagc
    31261 gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc gtttagttga
    31321 agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta gacaatttat
    31381 accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc ttgactgact
    31441 cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa ttgcaagtcg
    31501 atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc tccatcagta
    31561 cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa gaagcagaac
    31621 atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat cccgaccaat
    31681 taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc aaatgtgcga
    31741 cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca gctttccatc
    31801 attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga atataaattc
    31861 gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac agaagaagtg
    31921 atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt ccgtctgaaa
    31981 tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct ttcagacatc
    32041 gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg tgactgggaa
    32101 gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga agctgaacga
    32161 gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca taatatgaaa
    32221 gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt gaaagaatac
    32281 cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa caaattagca
    32341 gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa aggcgtcaaa
    32401 gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg gcaattcgaa
    32461 cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg ttgtacggca
    32521 cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac aagaaaagat
    32581 gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa cttagtaacc
    32641 aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa cctaggtgca
    32701 aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg aggcaccatg
    32761 ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa aatgatccac
    32821 aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga cggcggagaa
    32881 gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat ctctcatatc
    32941 gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc attcaatgag
    33001 atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat ctcagaagaa
    33061 caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga aatgatcgtc
    33121 atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt cgaactcaat
    33181 cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag gagctagaac
    33241 ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa tgaccagatt
    33301 ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct gttttcttag
    33361 gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt tcatgcccta
    33421 atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta gcatgtgtat
    33481 gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata tatcttaacc
    33541 aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa acatgttgct
    33601 ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt aatttccagt
    33661 tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa gctcttgttt
    33721 tcggagattg aattataact ttaccaaatt catctacagc aagagtttta ttgacatgta
    33781 gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca ctctttctca
    33841 tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca aaatctttaa
    33901 aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt ttctcatact
    33961 ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat tgcattgcaa
    34021 ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt tcatatttac
    34081 tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat agtttaagct
    34141 taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg attgttgaag
    34201 ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca aatgtcatat
    34261 caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca taatcttttt
    34321 gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca ccagttcgtg
    34381 gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc tttttgtact
    34441 ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt accactagat
    34501 ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg aaatcgtttg
    34561 ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat aagacatgtc
    34621 tttcaattga ataggtagcc attcatctac cattctttct agtagttctt ctatactgct
    34681 agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga ataattcttc
    34741 tatttttaag atattgaaaa gactaatttt ttcattttta actttattac aagtcttgtt
    34801 agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc atgtatctaa
    34861 attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt tatctggaaa
    34921 tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa tatattcttc
    34981 tacattgcca caaactaact cctctatact catatttccc aaatcagcaa ttcgggataa
    35041 tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt ttttgtcaat
    35101 taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt tactctttct
    35161 tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta ttggtggacg
    35221 ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg gtgttgtaaa
    35281 taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt atgtcgatga
    35341 actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt attttgagca
    35401 aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg agggggaaat
    35461 atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca agtgaaagag
    35521 ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac tctagagtat
    35581 atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat tagaaaatat
    35641 gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt taaaaaatct
    35701 gatattgata gtttcatgga acggtataaa ggttgagtaa acaaataaga ctaaaacata
    35761 tagtaataaa gagtttgttt ttattatctg tatgttttag tctttaacag aattttataa
    35821 ggaggaaaca caaatggaaa catcaatgat taatattcca atttatgtag atcaagaaaa
    35881 attattagag aagccagtta caaaaaaaga aaaaactgat gtaagagata ggtgtttaga
    35941 taactataga gaagttacaa gagatgagtt tgtagagata ctaaattcat cacaatcatt
    36001 tattccaagc aagacaaaga ataaaggcaa tgacaaggag agttttgtgg aaaccagagt
    36061 aattattctg gatgtcgata acactgttaa agatgagaag gggaaggtag tagatttgag
    36121 taaagatgat agtagatact taagcataga aaaggtttta tctatcaatt tagttcataa
    36181 ttctgcgttt gcgatacaaa aaagtattcg gtactctgaa aatctagaaa aatacaagat
    36241 tgtctttcta ttaaaagaag caattactga ctatggtaaa atggttgctg tttacgaata
    36301 cttgaaaaag aacatccccg ggtgtgatga caatgtaaat gcttcgaata gaatgttttt
    36361 tggaggatat aagtcagatg cagtgataat aattaatgaa gacaacatgt tagatattac
    36421 tgagcttccg attgatttta ccatgacatg ttctaaaaat tgtcaaagtg attcagggag
    36481 agagtatgaa ctagtcaatg aaaccgattt tgttaagttg ataaaaaatg acgacaaaga
    36541 agaaatgaaa cagtggttta aagactgcct gtttgattcc tccgacatgt cttttaatga
    36601 gatttatgaa agattgttaa ccattgatat gaataaatta cttaagtcaa gtaaaaactt
    36661 aagatgtctg tttcacaacg atcataatcc ctctgctagt atttttacca gtaaaactgg
    36721 gcattccatt ttttattgtc attccagtaa ttgtgatatc agtgaaaatt ttattggagt
    36781 tgtgatgcta attttaaaga aagacagtcg ggtagaaaca tttaattggc tattagagaa
    36841 cctagacttg tatcctacac attttcgaaa attgaaagag gaatcaaaag tactttttga
    36901 cactcttgaa tcaatgaaag acaaactttt caggacaata aggaattatc tagaggaaat
    36961 gcgacgtgtc tatgatgtct tgctttctga tgttaatttt tttgatgata gtaaggaaag
    37021 tgtgacttgt attttgtcgg gggaacaatt agctaaaaaa atgagttctt actatggtaa
    37081 aacttatgac atagataaat gtaataagtt actatcactt atgactttta taggattgat
    37141 aaaaaaatta gatgatgaag atgttccttt gaagatgctc gaatacctaa ataggttaaa
    37201 ggaagagaaa gcgttaggaa actttaatca taagagaagc aatgtctatc gattagacgt
    37261 ttttaatgca acttctgtga ttcaaaaatt gaatgaagat atccttccac ttctggaaaa
    37321 aattcatttt acatatcaat atttttctta tgaatgggtt aagatatgtt ttaacgaaaa
    37381 agaggctaaa agagtatttc ctcaaaattc ccgatcagaa ctcagcaagg aaaaacaatt
    37441 gattttagat gaagcacatg aagttttaca gggcttatcg tggcaaggga catatctaat
    37501 gacagagaaa gaacttatta acactgtttg tgaacgccta cgtaatgttg gggataacag
    37561 cgtgaaaaat gctttaataa aaaacagagg ctactttgtt aaacaaggat tcactcttga
    37621 acgagcttct aaggatatta agaaactata caatgtgaaa gcaagtagta gttttttaat
    37681 atatttagcg aaaggggcag ggatagatga aacaataaaa gaattgatat tatcaggagc
    37741 tgggatagtt cctctgtatc aatttacaag tgtaacaaga gatgatggac ggagagtaag
    37801 gaagagaaaa gacccaattg tattaaacta gacaggttat ccatagctct taatcttatt
    37861 tcatctcaat acacccaccg ttaaagttcg gtgggttatt tatttttcaa tctttcaata
    37921 gctgattgcg ttcattttct tgccttttta atagcttcat atagtcaatt gttcgaactg
    37981 catcttcagt gctaagagaa cgatagagag ccaaaaactc ttcctcataa tctacgacaa
    38041 cattgtctag aaaatggctt acaggggttc ctagggctct agaaagagct gttaagctat
    38101 cgaggttagg ctctgttaca gaattttccc atcttgctac tgcctgttgt gtagagaaga
    38161 ttttttcagc taattctttt tgtgtccacc cgagagcttt tcgcttttct ctaatgacct
    38221 ttgcatattg tttctgtttc attaatacac ccccatctca gtatagtaca acaagttgtt
    38281 gtatttgtaa tggtatttga agttttttat attttatgag ttgaaataca tcatattgtt
    38341 gtatataata aaacaggagg ggagaatcat gaaaaaagta aataatctca gccgatacag
    38401 aaaagaaaaa ggactttctc aaacggagtt agcaaaaaaa atgaatgtta cacagcaatg
    38461 tgtaagttcg tggcaaacag ggagaacgat tcctaaaccc tatcaaatga agatgctttc
    38521 tgagatatta agtgtaccaa tgaatgagct gttttctgat gtatttaata aagtaaacag
    38581 ttaactaata agcaattgaa tgattttagg aggagaagac atgtattgta gaaaatgtgg
    38641 caaagaaata acagatgaaa gtaagtattg ccagttttgt ggagcactac aagaaacaga
    38701 tgagacaacc ggaacagaca cagtaaagaa aagtactgag cagacaatta atgaaatgaa
    38761 ggaacaaatc gtacatggac aagagacagg gaagctgtat ttgattattg gttggatttc
    38821 tatggtagta tcgttagcat ttattcctgt tgtatttggt gctgttgctg ttattatggg
    38881 ctacttatat cgagaaaaag atgagaaaat gggtacgatt ttgatgatag caggggtagc
    38941 tggagcaatc tttggtgtac ttttagggat gtcaatgtac tgataagagg gataagaaca
    39001 aattattttg gagataggag ataaaaagtt gaataaagta aatatgaaca agaagtttgt
    39061 cattataggt attgttgtct tggctattat aggtattatt ttttcaaaaa tgacaggtac
    39121 atctggatta gagaagtcat caaaaccatt ggtaacacag attttacaag aacagtacaa
    39181 cttaaatcga tcatgtgatg atgtgacgat tactcatgaa aatggagata acacataccg
    39241 ggctaaagct atattagaca atggaagtgc tatcaacata aatattgagt actatccaaa
    39301 gaaagaccgt atatatgtgg agataccata tgctgaggtg ttaatgttaa attgatttaa
    39361 atgtattatg cataccgtcc aaacaggaac tcccaaaggt gtaaattcac tagggagttt
    39421 ttatagtttc ctataaatta aatactaata caggtgacaa aaaaatatta caatgattgt
    39481 cgggaatttt tatgttattc actgttataa caatataaaa gaaatgaaaa agaaaaaatt
    39541 attatggata tgtttggtat ttttattgat aattggtgga att
//
LOCUS       DASHFF010000021        38222 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.021, whole genome shotgun sequence.
ACCESSION   DASHFF010000021 DASHFF010000000
VERSION     DASHFF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.021"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(578..1522)
                     /locus_tag="U2I75_001223"
     CDS             complement(578..1522)
                     /locus_tag="U2I75_001223"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1696370.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1818..2762)
                     /locus_tag="U2I75_001224"
     CDS             complement(1818..2762)
                     /locus_tag="U2I75_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1696371.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2983..4197
                     /locus_tag="U2I75_001225"
     CDS             2983..4197
                     /locus_tag="U2I75_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1696372.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4219..6234
                     /locus_tag="U2I75_001226"
     CDS             4219..6234
                     /locus_tag="U2I75_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HEN1696373.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6317..7690)
                     /locus_tag="U2I75_001227"
     CDS             complement(6317..7690)
                     /locus_tag="U2I75_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1696374.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7716..8000)
                     /locus_tag="U2I75_001228"
     CDS             complement(7716..8000)
                     /locus_tag="U2I75_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1696375.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(8002..10017)
                     /locus_tag="U2I75_001229"
     CDS             complement(8002..10017)
                     /locus_tag="U2I75_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1696376.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10196..10501)
                     /locus_tag="U2I75_001230"
     CDS             complement(10196..10501)
                     /locus_tag="U2I75_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN1696377.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10590..11621)
                     /locus_tag="U2I75_001231"
     CDS             complement(10590..11621)
                     /locus_tag="U2I75_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1696378.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11828..12703
                     /locus_tag="U2I75_001232"
     CDS             11828..12703
                     /locus_tag="U2I75_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1696379.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12855..13697)
                     /locus_tag="U2I75_001233"
     CDS             complement(12855..13697)
                     /locus_tag="U2I75_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1696380.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13789..15666)
                     /locus_tag="U2I75_001234"
     CDS             complement(13789..15666)
                     /locus_tag="U2I75_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1696381.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15663..17084)
                     /locus_tag="U2I75_001235"
     CDS             complement(15663..17084)
                     /locus_tag="U2I75_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1696382.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(17087..20476)
                     /locus_tag="U2I75_001236"
     CDS             complement(17087..20476)
                     /locus_tag="U2I75_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN1696383.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDDYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20733..22124
                     /locus_tag="U2I75_001237"
     CDS             20733..22124
                     /locus_tag="U2I75_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1696384.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22197..22844)
                     /locus_tag="U2I75_001238"
     CDS             complement(22197..22844)
                     /locus_tag="U2I75_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1696385.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22852..23538)
                     /locus_tag="U2I75_001239"
     CDS             complement(22852..23538)
                     /locus_tag="U2I75_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1696386.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23544..24617)
                     /locus_tag="U2I75_001240"
     CDS             complement(23544..24617)
                     /locus_tag="U2I75_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696387.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24839..25513
                     /locus_tag="U2I75_001241"
     CDS             24839..25513
                     /locus_tag="U2I75_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1696388.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25518..26858
                     /locus_tag="U2I75_001242"
     CDS             25518..26858
                     /locus_tag="U2I75_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1696389.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(27007..27993)
                     /locus_tag="U2I75_001243"
     CDS             complement(27007..27993)
                     /locus_tag="U2I75_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN1696390.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28717..29748
                     /locus_tag="U2I75_001244"
     CDS             28717..29748
                     /locus_tag="U2I75_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1696391.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29857..31389
                     /locus_tag="U2I75_001245"
     CDS             29857..31389
                     /locus_tag="U2I75_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN1696392.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31528..32340
                     /locus_tag="U2I75_001246"
     CDS             31528..32340
                     /locus_tag="U2I75_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN1696393.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            32330..32815
                     /locus_tag="U2I75_001247"
     CDS             32330..32815
                     /locus_tag="U2I75_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1696394.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32838..33383
                     /locus_tag="U2I75_001248"
     CDS             32838..33383
                     /locus_tag="U2I75_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN1696395.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33456..35099)
                     /locus_tag="U2I75_001249"
     CDS             complement(33456..35099)
                     /locus_tag="U2I75_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1696396.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(35228..37456)
                     /locus_tag="U2I75_001250"
     CDS             complement(35228..37456)
                     /locus_tag="U2I75_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1696397.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37489..37968)
                     /locus_tag="U2I75_001251"
     CDS             complement(37489..37968)
                     /locus_tag="U2I75_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696398.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11168 a         7211 c         6942 g        12901 t
ORIGIN      
        1 ttcataaaaa tatggaaaat atggattaat tttgaaataa ttgatttaaa aggaatacaa
       61 aagagtgaat agtaggaaat agtggtttag aactagtggg ctgatttttt atatatttta
      121 gtttacataa tatatattat acaaagtaga tttaaaaaat gataaacctc tctcgaacgg
      181 ttttttatca aaaatcgtca tttttccatc aaaatctgct tatgcaaaat tataaaaaaa
      241 ttttgcagtt tttcttactt tttttgtcgt ttaattgaat gataaaaacc agtttcttta
      301 ttcagttaat caaatatgaa cgaattagat atgaatcatt gtatttgccg ttgcggaagg
      361 attttttaat taaccacaga taaacattga taacgattcg cttattgtaa tcaacagatt
      421 aaatttcata tgttcatatg tacggataca ttttcggacc gattaatttt aaccattctg
      481 tttatttgct tacctattag atatcaataa aaaactgctt aacagctatt tcagctgtta
      541 agcagtttta ggatttttag ttcatattca ttgtttctta taatacagtg ttcaatactt
      601 ctcttaaagc acttgttgat ttgctgaaca ttgctttttc ttcttcgttg attgataatt
      661 caacaacttc acgaacacca ttttcgtcaa cgattgatgg cacacctgta aagatatctt
      721 tttcgtcgta ttcaccagtt aaataagcag atacaggcaa tacagcttgt tcgttattca
      781 agatagcttt tacgatacgt gcagtactca taccaatacc atagtaagtt gcttttttac
      841 gatcaatgat ctcataagca gcattacgga ctttatcagc aatcacatct aattcatctt
      901 gggcaatacg atggtctttt tctacaattt caaacactgg tttaccaccg acagttgtat
      961 gtgaccatgc agcaacttct gaatcaccat gttcacctaa gatatagccg tggacgctgc
     1021 gtgggtcaac ttttaatttc aatgcgattt ctttacggaa acgagttgta tccaatgttg
     1081 ttccagtacc gataacacgt gatgttggca gaccagattc gttccaagcg atatatgtca
     1141 aaatatctac tgggtttgaa gcaacaacga taataccgtc gaatcctgat cccatgattt
     1201 cttttacgat ttggcgcatg attgacgcat ttgttttaac taaatctaaa cgagattgac
     1261 caggtttttg gttgatacca gctgtgataa caacgatatc agcatcttta cattcttcat
     1321 agccacctga ccatacagcc acattttcat cgccccaagc cataccatct aaaaggtcca
     1381 atgcttcccc ttctgctttt tcttgattca catcgatcaa tactaattca ttagagattc
     1441 cttggttgat cattgcataa gcgatacttg ttccaacaaa acctgtaccg acgattacta
     1501 ctttacgact tgtttttttc atgaataaac agctcctttt cataactatt tgttactttc
     1561 atgaaaagta tagtcttgtt tattcatttt atcaacatgt taattataat agttgttatt
     1621 ataatttttg atggaaacga attcctgaat acgcataaca gataaatgac aaatgtttac
     1681 acctatatcc cctgataata caaccaaaag tttagaactg aaaataaatc caaaaaaatt
     1741 cgctgcaacg atataataag acagcaatcg cacctaaaac aattttcctt catatgcttt
     1801 agtgaaagat tttcttctta atgtttattt ctccattcat tgggagtaca tgttgaatac
     1861 ttcttgaata atttgaataa agaagtcgtt tgtttatatc cgatacgatt agcgatttct
     1921 tccaacgaaa aactcgtttc ttgaatcagt tccaacgcaa agtcgaattt tttctttaga
     1981 accagttctt gaaaggaagc atcaaaatac attttcaatt tattactaag atagaccgga
     2041 ttgtagccaa aatgagctgc tgtttctttt aacgtacaag aagtaaaatg cttatcgata
     2101 taatctaata ctctaagcat ctcattttct gcgcatccat ctgctgatga aaagctttgt
     2161 tgttctaata aatgagtcaa ttcgatcagt aatagtgatg tatagagatt taacgaacgg
     2221 atcttatgac tgtatgtatc aaaataaatc aacagcatat tttcaatcaa atgcgtcaaa
     2281 cgacttgttt ttccgccgcg aaaatgaatg aattgtgtat gttccccttt ttcttttgat
     2341 gcatttatca aaaaagaaga aagtagatta tttgtgcttg ccaaatgatt caatatctct
     2401 gtagataagg tttcttcttt taataagatg ttgattaaaa tatcctcttg ccctgtgtat
     2461 tcgattcttt gagtagcttc tttatcaatg atcaccacgt cactttcttc catctctatc
     2521 ctttctccat taataatttg taaacatgta ccgctataca tataattgat ttcaacaaaa
     2581 tcatgtgtat gctcgggaac ttccgcatat cgacggtgtt tactgatatg aatatttccc
     2641 tgttgaaata gatcccctcg gtggattttt ggcggtgtct gaagatggta agctaagttt
     2701 aaatcattga tattcattcc ttgttcacgc tgcagagttt cgattgattc ttcgttacgc
     2761 aaaaaaaatg tctaattcag ctagttccaa ttgactccct cctttttgta agcggtttct
     2821 ttcgcttatt agttcacgct gtactgctat tatatgcaaa gctatttttg gtttcataaa
     2881 gactttctat ggttgggaca tgaacttcct gtgattccgc tattgagaga ataaccactt
     2941 catactatat ttttgataag cgcttacaaa aatgaaagga acatgcttat gaataaaaag
     3001 atgatgaaat tttctctgct gtcagtctct cttattttga cttctgctgg tgcagtcacg
     3061 tctgcaattc ccagtatgtc agctacttat gccacgcgtt ctttatctgc tgttgaatta
     3121 ttaacgactg ctccctcttt tatggtcact atttttgttt tgattagtag ttttattgct
     3181 aggaaaatcg gacaaaaaca aactgtgatc atcggattag tgattgcatc gatttatggt
     3241 atcctccccc tcttctcaca aagttacagt attgtcttag cttcaagagc tggactaggt
     3301 attggctttg gattgatcaa ttctttggca gtcagcatga ttgctgcctt ttatcacgga
     3361 gatgaacgag cctctatgat tggttttcaa agtgcatttc aaggatttgg tgcagcttta
     3421 atgacatttg ttgcaggaca gctagtacag ttcggttggc aatatacctt tttagtctac
     3481 gccatcagct tacctattct tgtcttgttt gttttgtttg ttcctacacc tgaaacaact
     3541 gaacttcaat cacatggcac agccccaaca agacaagtaa tcaaaacgaa tcctaaaatg
     3601 attagttatg gtatttttct cttgctggtc atcgtggtat ataatgctgt atcgattaaa
     3661 ttagccacag tcctgacgac ttattctcta ggaaacgaaa ctaatgcagc gaatatctta
     3721 acaatcatgc aattggcaag tatggctgcc ggagtttgtt ttggctggct tcagacaaaa
     3781 gcacatcgct atatgcttac actttcttta ctactgatgg gaatcggctt tgtattgatt
     3841 gccactgtga taaacctgta tgtgattggg tttggtgcga tcctaacagg aatttctttt
     3901 tcgttattca ttccttactt attcaaccaa gtaagtatcc aagcgcccaa gtacgcggag
     3961 agtttcaata cttcaatctt attggttggc gctaatttag gtactttcat tgcaccatat
     4021 ggtttgctct ttctctcttt tgcttctctt gggagtaaaa cacttgctgt tttcgtcaat
     4081 ggcagtatct tgctgttgat tagcgcgctt attagccttc tagtagtaca caggacgaat
     4141 agaaagacag tgataagagt agatgaagaa ataaaagaac aattgacaac aaaataaatt
     4201 tgatggaggt cgtgtaaaat gacaaatgaa aaacataaag atagatggct ttggtatcct
     4261 ggagactttg agatccgcca tggactgcta caaaatttcc aacgggaaga acgaggtttt
     4321 gactggccag cgtactggta catggatgat tgccatcgga acgtcaagtt taagagatat
     4381 tactttcttg atcaaccttc catgtttaaa gtgacgatcc aaggagttgg gtatgtagaa
     4441 attaatggac aaaaacatcc atgtggaaaa tggctgactt gtccagctgg taaagcaaaa
     4501 attcgtatct ttgtaggaca tacatcaggg ctaccagcga tgtttatcga gggagacgaa
     4561 gtaaaatcag atattggctg gacagcaagt aattttattg aagaatatcc agctggctgg
     4621 agtccgcttt atgtcgatat cgcaaaagat ccaaatcaaa tctattatca aaaagaatgc
     4681 gtccatccag tcactgaaac ggagattggc ggaggaatct tattcgattt tgggcgagca
     4741 gtgaatggct gtgtagaact aatatctctg gatgaccata tcgaatctat cactctatgt
     4801 tatggagagt ccgatgtaga agctatggat atcgattatt gctactataa gcaaatagga
     4861 atgacaagaa aaatgaaagc tcgtaaacgc gcttttcgct atttgtttat accagaggtt
     4921 tctactggaa tggttcaact tcaagttttt catgaatttt tatctctttc gaaacaagct
     4981 gctttttcaa gcgattcttc tttactgaat cagatctggt cagtatcaga agaaaccttc
     5041 aagttatgta gtgatttgtt ttttatcgac ggtatcaaac gagatcgctg gatttggtct
     5101 ggagatgcgt atcaatcatt catgatcaat caatatcttt tttttgacga ggaaatcaat
     5161 aaacgaacga tattagcatt aagaggacaa aatgaaatca agcagcatat gaatactatc
     5221 gttgactatt cgatcttgtg gttgattggt atcgaaaacc actatatgat gacgaaagac
     5281 tatgaatttt tacgcatcgt ctaccctaag atgcaatcat taatgagata cttgatggag
     5341 caaacgaatg aattagggtt tatttatggg agagcaaaag attggatatt tattgattgg
     5401 tctgaaatgg ataaagaagg aacgcttgct gcagaacaag tcttattact gaaagcctat
     5461 cattccatga tcacttgcgg agatatctta gctgaaacat atggagagaa cgaaaattat
     5521 catactcatc tctatcaaga aaaatatcaa ctattgaaac aaaacctatt tgactacttt
     5581 tgggatgaag aacttggcgc ttttatcgat tgctatgaat ctgggagaag aaatgtgacg
     5641 cgtcatgcga atattttcgc tgttttattc gattttgcta acaaagacca acagcaatcg
     5701 atcttaaaga atgtcttatt gaatgacact atcacacaga ttactactcc atatttcaaa
     5761 ttctatgaac aagatgcttt atgcaaactc ggacagacga atatcgtcta ccaaacgatt
     5821 cttgattatt ggggagggat gttatctcat gatgcggtaa ctttctggga agaatacgac
     5881 cctactcaaa caggaaatga aaagtacagt atgtatggag atccttatgg aaaaagctta
     5941 tgccatgcat ggggagcaag cccaatctat cttttaggac gctattttat cggtttgtat
     6001 ccaacaaagc caggttatga agaatttatg atacaaccta atctagattc atttgaaaca
     6061 ctgaattgta cgctacctat caaagatgga aagatttcgc ttgaaaaaac aaaagataag
     6121 atcgtgatta cttctgatag aacaggcgga acagtgaaaa tgaatggtaa aagttatcct
     6181 cttctagcaa aacagccact tgtcttaaca agctattcaa aaataaatgc ataaaacaaa
     6241 aaactgcttg cgacatatca gaagacttta aatcttcttc tgatattgtc caagcaattt
     6301 ttattttgaa gtttgcttaa ttctgttctt tagaaacaat gtttgttctt tttccggcaa
     6361 aaagaaagct agataatacg acaagtaaaa agaaagcgat gattccaact gtcaaggcta
     6421 tttgaccgta ttctcctagt ttcccaagta caagtcctgg tactagataa tcagtatcgg
     6481 caaatgtaga agaagcgatt ttaagatctc ctaatactgg aaggataaat aatggcagcc
     6541 aactaatcaa taagctattg aaagcagaac ctaataccgc ccctcttcta ccgccagtcg
     6601 agttgccgaa gacaccagaa gcagctccgc agaagaaatg tcctactacc ccaggaatga
     6661 taactgtagt ttgcaagcct atcatagcaa acatggatag tgttcctaca aagaagctaa
     6721 caaaaaagcc aattaataca gcattaggtg catatggaaa aacgatagga acatctaatg
     6781 ctggtttaga atcgggtacg agttttttag caatcccttg gaatgctgga acgatttctg
     6841 ccaacaccat tcgaacccct tgaaggacga taacaaaccc agctgtgaat gttccagctt
     6901 ggatcagtgc aaagattaca gcgttgtctc cattgcttag ttctctttca acaaatccag
     6961 gaccagctaa caaagcaagt gcgacatata cgatcgacat caagagcatg atcgatacag
     7021 tagaatctcg caaaaagctg aaacttttag gaatctttat gtcctctgta gaacggtctt
     7081 tgtttccaaa aaactttccg atccatccac tagaagcata accgatattt ccggtatgcc
     7141 ctaacgcaac ttgattgttt ccagtgatgt tcttcataaa cggttgacaa agagcaggcg
     7201 taatagttaa tagtgttcct tcgaagaccc cgcctaataa gatcatccac aaatttgcag
     7261 taaaccccgc gcttactaaa atgactgctg ttagacaaga aacataaagc attgcttgac
     7321 ctgtaagaaa aatgaattta tatcttgtga atctagcaag taaaatattc acgatcatcc
     7381 ctactagcat gatcaaagca gtaggtgtac cgtattgttg caaagcaatc gccactactg
     7441 cttcattgct aggaactact ccttgagtat gcagagctgt ttggaacatc gaagcaaaag
     7501 gggttagtga attactgatt aaaccagcac cgccgatcag tactaggaat ccgacaaatg
     7561 ttttgatagt tccttggatc acttctgaaa atttcttctt ctgaagaacg agaccaagca
     7621 gtgcaatcaa agaaacgagg atggagggct gactgacgat tgacacaaac aaattcaaca
     7681 atttatccat tgatagttcc tcccttgaat catttttatt cttttgacaa gatcttcgtt
     7741 actttttctt ttacttcttc gctgtcaatg atcgattgca aaggtacgag cttttcatca
     7801 ggtatagaag ttagcgtatc tttcaaagaa tgctcaatga agaaataatc agccatatcg
     7861 ctagatacac ttccaacatc cgtatgactt acttcaaact cgtccacatc tgctccaaga
     7921 tctgcaagtg cttgtttgat gttcatctct accataaaac ttgttcctaa tccactttga
     7981 catactgcaa aaattttcat atcattcgtc tccttttctt atgatcgcta atatttcttc
     8041 ttttgttgat gcgtgtaata aatcttgaag attttgttta ttagaaagta gctttgttaa
     8101 atttgccaaa gcccgaagat gtgcctcatt atcgatagca gctaaacaga tgaacaaact
     8161 aatgggatgg gctggattat caagcagatc cacaggttgt tgaagtttca gcaaagacat
     8221 ccctacttcg tttacgccat cttctggacg agcatgaggt aatgcgatgt gatcgccaat
     8281 atggataaac gcaccatact gctccacgcg ctcgatcata gctttgatat aactttcttc
     8341 tattttattt tctgataata gcggctgtgc ggcaaatgtg attgcttctt gccagttttt
     8401 tggttgatcc gataattgga tcatttcttc tgttaataat tcagatagca taggacgttg
     8461 atcctccttc ttttctaaaa gtttgttcat tttacgattt aatacttgat agagcttttc
     8521 ttcgtctacg ccttctttta attcgatata aggcagtagc gccgacaaca attcttgggc
     8581 agaaggcatt gaaaatcctg gtatcagcca atctctttgt acttgattga tcagctgatt
     8641 tttttccaag tcagtcatga ttggatgcag aacatagaga gactttttct tatcagtaag
     8701 tgtgagaggt acagtagaaa aaataatatc gtaagaagtt tcgtcaacct cgtgtaagcg
     8761 gtcagctgaa ttagtctctg taaaaaagat catcgggaat aattgctgaa gttcactttt
     8821 taagatcaat gaagagctaa tgccacttgg acagacgatc acggctctta tttttctatt
     8881 tctttcttct gcgtccgctt ttctgatctc tccgccaaat agtatagtga aaaaaccaat
     8941 ctcttcttca gggatgctct tccctattcg tctttctaac ggaagtaatg cttttcttgt
     9001 catttcgtag atggaagcat attgatgctt aatttgatcg atcatcacat taggtaaata
     9061 aaaagaatag gaaatacgaa aataggcagg gaccaagtga taaaagacat tcatcaataa
     9121 ttcacgaaac gaagcaaatt ctacggccat caggaattcc atgcgatgga tgacttctga
     9181 agaacattcc agcaaaaaat caagtgcagg ctcctggaca tctccttcaa cgatcgatgc
     9241 taagcaaaga gagaaaaata taatctccga attttgatgt tcggaatagc caaggccagt
     9301 caatacttgt cgactaatgt cagtgactgc aagagaagcc aacagttcag catcttttac
     9361 cagttccatg gatgtttttc caattcgaat ggttgtcaaa ctcaaataat aagctaattc
     9421 atccattcga tttggtgcga atcgaagatc tgatagtgaa atcgttttat ctagcaaatc
     9481 tctaactttg gcgtaataat caatggaaag ggttgcggtt aaatacgtga gcccaaatct
     9541 tccagcaagt gactgcatca aacgagcagt gcgatttttt gcttctcttc ttaatattgc
     9601 ttctgaccct agcagcctaa aaccagtttt cctagtatac gacagctgac aatcttccac
     9661 ttctaattca tttcttatct ttttgatatc tgacaggact gtccctttag aaacctgaag
     9721 aaaatcttga aaatgaaaaa cagaaacaga cgttgcagtc gaataaacca tcaaataaag
     9781 aagatctctt cgctcttgtt cggtatacac agcttcaaat cgagtaatat ccaaaaattt
     9841 ttctgcccag tcgattgctc ggaatttctt tatacggata cgggtatcat ccacatattc
     9901 ggcaaaagga gttaattgtt tgatcgcctc gcttgtattt acttcgatga tctctgctgc
     9961 tttttctaga ctgatttctc ttggtgtttt caggagataa ctaattttgt agttcacagc
    10021 gctgttcacc tcctacttat attctagtat ggtagcgctt ttaaatcgag gggtgtttgg
    10081 tacaacttac agttggattt tttttacgac tgtaccgaac gatttctatc gaatggatac
    10141 agaaaaaaag caggtgacca aatgtctcct gcttttatga attgatttcg tccgatcaat
    10201 gatgatcatg tttatttttt gaggtcagtt cttcaaattt ttctttgaca tcttcaaccg
    10261 ctttttcgat atctttttca aagtgattgg gctcattagg ctttgtttcc tcttctacag
    10321 aatttgaacc agataaagct gtttctttat tttctgtttc accagagttt gtgccagcta
    10381 atgattcttc tttgtcttct gtttcaccag aattagttcc tgctaaagcc acttcttctt
    10441 ctgttttagg ggccattgaa cctcttaatc gtttgtgttt ttcaccgtca taatttgtca
    10501 tgttggttcc tcctagtagt tctatacttt ttcatcgtac aataagacag tttatgcgtc
    10561 aaaaaacaaa cccgattctt acaaatatgt catatttttc cttctgattc ttgaatgctt
    10621 ttgattcctt cttccaaaat tttcatttgt aagattgttt cttcatcttt gatgactttt
    10681 tctgcgccat taactaatgt ctcgtaaatc ccttcgtaga cacggctgta atcaccaacc
    10741 tcggaaatta ctttttcttc atgataactc ccttgttcat ctatataggt caatacacca
    10801 taatgttcgg gcaaatcgat accaaagtct tcatgatcag gcatgtaaaa cattttcaga
    10861 tgttcttctt gtcgatcttt tgtttgtttc acgaacatcc ctttttttcc gtaaacgacg
    10921 aaacttggac gttctttgat gcggaaataa ctggatttta tcgaaatttt catatttcca
    10981 tagaaaaaat ccagatcgaa gtaatcattc attcgtcctt tccctaaaag ttgacgtaca
    11041 tcatagctga tatcatctgg ttcgccgaaa taagaaagaa cttgatctac tgtatgacaa
    11101 gcatgtccat ataaatagct gttcgcagta gaaaattcat gcgtattttc tggtacttcc
    11161 ggtctgaaat aatcataatg catttctact tctaaaatat cccctaattt tccgctttcg
    11221 attacttttt ggactgtcaa gaagtcagaa tcgaaccgtc tgttttggta acattggata
    11281 aatagacctt tttcttttgc taatgcaaat aactcttctg cttcctttga cgtttctgca
    11341 aaaggtttct ctactagtac attcttacca tgttcgagtg ccatttttcc atattcataa
    11401 tgaaattgac taggtgtagt gatgacaact aattggatct caggatcatc ccaaagatca
    11461 gacagctgat cagtatattc gatgtcttcg aatgttttcc acactggttc agatggggaa
    11521 aaaatttttt tgatattgat tttgttcgta agttttcttg aaaaaggcaa atgatagcga
    11581 tttgtactct ttccattgcc aagataagcg attgtaagca tgagtggtgc ctccttttat
    11641 tgatgttttc agtatatcct agaaaagcgc agaaagtatt tataatagct attctggtaa
    11701 aaaagttcag aaaaacactt gttatcattc ggtttggtga aattaatgtt caaagtaagg
    11761 ataaaaatca aaaagatagt agcggaacca agtgtttatc agtaagatag aatttggagg
    11821 tcagatcatg cttattgaag aaaaactaac aaaacaagag ttgtttacca cgacagagaa
    11881 acggattgtc gattacatcc gtaggaatat cgaagctgca gtctacatga cgattgaaga
    11941 attagcaaaa gctacgtata cttcccattc agctatcatt cgtttatgca aaaaaatggg
    12001 attcagcgga tttaaagaat tccgtttcga gctggggcgg gaagttcatc aattgattgc
    12061 tcagttcaat caaacgattg atcctaattt tcctttttca agtgaagata gcactcagca
    12121 aattgcaaag aaaatggcgg atttatcgat ccagtcaata aaaaaagcac aattgcagat
    12181 tgaacatgaa gatttagatc aaatcggtaa agtattgaac aaggcacagc gaatctttct
    12241 ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc cagaataaaa tggtcaagtt
    12301 gaataagttt ttgatcatgg ctgaagaata cagtgattct tcttggaatg cagccaattt
    12361 gactagcgag gattgcgcca tttttattag ctacagcggt cgcatccatc attatgaacg
    12421 aataatgacc tatttgaagc atgtagaggc ccctaccttg ctgattaccg gaaatcagca
    12481 ttcagaaatg gccaaacagg ctgatatgtg tcttgttata tcgcaggagg aatatgattt
    12541 tgctaaagta gcgactttct cctctcaaat tgccttcgat tacgtattaa atacgttatt
    12601 ctcagtgata tattctcaaa attttgaaga gaatatccgc aacttgaaaa acaaacaaga
    12661 attgatgcaa aaaggccttc ttaaagaata tcggaaaact taagaaaaga tcctcattta
    12721 gtaataagaa agatagtgtt tgataggctc tctttgatat catcataaaa acaggctgaa
    12781 gttttctttt tcaaacctca gcctgtttgt ttacggaacg attcgaatag atatattata
    12841 gaaatgagga atagctaact tctgtttctt attttgaacg attgacgaaa aagatatata
    12901 agaaggagga aaatcagtat acatgcagct aacaatccat acaggaaaaa tcgtttgttc
    12961 tcggatactt tctgtacttc tttttgaact tcttttgcat gataaggaac ccggtgccct
    13021 ctgacaagta agcgatgact gttgaccata taaggtgtac aagttaggag tgtcacgaaa
    13081 tcctgccctt tttcgattct cagatctttt gtatcagtcg gttctaccgt cttgatctga
    13141 tcgacttgat aagcgagtgt ccgtccattg atttcgatat agaaagaatc acctaaagtc
    13201 agtttcggta agtctgtgaa caactttgct tgaggcagtc ctcgatggct ggaaataacg
    13261 gcatgtgtat tttctccccc cgtaggataa gacgttcctt ccaataatga ggcacctttt
    13321 tctagcagca cagtattcgt ttcatcaaaa attggcaaat ggacattgat ctcagggatc
    13381 gtcaaaatac cgatcgtatg tttctcaaaa taggttctat cctttttcgc aggttctttt
    13441 ttcttagtaa agggatctgc acctggattt ccaccttctt ttgctaattg ctggtttttc
    13501 tttgtcatgt tttcttggat tttagccatg acttcttcat tttctttgac cgcttgttgc
    13561 tgataatgag agatgatctg ctgatccaga taattattca gcgcgtcgct aacaaatgga
    13621 taagctagta tccctattcc tgagagaagc aagaaaagca tcagcagatc tagaagtttc
    13681 cttttctttg gtttcatttg tgttctcctt gtgatgactg tttgataata aaagaaaaag
    13741 gagcatttgc gtaatccgct gctccttttt ccaattcttg caaaaagact aaatctgact
    13801 gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga aggactactc ctgcaccgat
    13861 atacacatag attcccttac cgcctgttga aggaagtgta cctttgtgtt tatttggtat
    13921 tttttcagga gaaatcaact gtcctgctgt tacataagat tgttcatcga tagtaaatgc
    13981 tacacggttt gttaatggaa catatttttc tggcgctttc gtttcttcca gatagtacgt
    14041 gccatatttt agacctgtca catcgattaa accgttactt gtggttgtaa aaaccgttgc
    14101 tgattctttc gccgataccc agctgacggc ttttgtggat gggtcgatcg ataaatattt
    14161 cgctgtgtca ctatcttgat cacgaacgac gaattctgct ccagcaagtg tttggtctga
    14221 tgtaacgtca ccatctactt taacaaatcg tttgccccca gtaacgacat cgactgacgg
    14281 tggtgtttga tcatttgtat gcccgttatc gacatttgct tggttagaaa atcctttggt
    14341 tggatctgct ttttcgttca aatgcatata gtaaacgaat ttcaatgtac cgccaggagt
    14401 taatgaagga atataattcg gatcaactga aaccgtgaat ccgtctgttt gctcagtgac
    14461 agaataattt actgggtcga tttctttatt tccatcatat aaggcataag catacgttcc
    14521 tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt
    14581 gtacttgttt tgcgttcctt ctttatcagc gatacctaat gggatattga cagaaatctc
    14641 atattgaatt ttttcaccga ttgcgacatc ttttccattc aattgaggtg ttgttttatc
    14701 aactttagac gtatcatttt tgatggtctt ttctactggt gtttggctat ttgccaggat
    14761 ctcaaaaggc gtgattgttt gcttttcaat catttccgca ttatcaggag cttttacttc
    14821 ttctaaatta tattttccaa cttctaaatg attaacgatc aactggcctt tttcaataga
    14881 tgcctcggca aagtcattgt tgccgatgtc ataagaatga ccagtaatga aatgtttggc
    14941 tttggtttga tcagttgtcc aagtgtacaa tccatctgtg acactttgga tgtatttttt
    15001 gacgcttggt gttccttctt ctttagaaat aataaattct gctccattta gtgcttcgtt
    15061 ttcggcagta ccgatttttg taactttcaa cgttccatca ttaccgactg tatttttagg
    15121 gtagagatgg atagtatcta gttcttctgt cccgtattta taagagccat ctgcttgttt
    15181 gatcatctca tatacaggga aagctaaaac catgtttgcg gcagctgaca ctccgtcttt
    15241 tggttcttct ttgatcgtgt agactgcatc tttcccattt tgttttttag gtaaagataa
    15301 agtgacattt ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga
    15361 ctggactgct tgttttgctg catccacgga cgctccttta tctcgttgcg cataaaattc
    15421 ttgagtgacg ttataaactg aaaatgaaat atcggctaat ccttggtatt gatcgaattc
    15481 gctcatttct ttcccgctgt tttggattaa aggatcgggt aaatcagtca ttttcttttt
    15541 atgaaggatg acttgagctg ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt
    15601 tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata acgtttattt ttttatgatt
    15661 tttcattttt tcactcctgt tctttttttg ataaacaacc agattgcaaa gcttgcgaat
    15721 accataccaa caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga
    15781 cggctatttt cttttttatt gactactttc aatatactgt ccgcctcttt atcctcagaa
    15841 agaaaagttg aaatggtaaa cggaatttct gtttcactta aaatataacc agaaggtgct
    15901 tcgatctctt ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa agcgccattt
    15961 ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg tccacttgta tccgttggct
    16021 gtttgctgga ggtaatttcc tgctttgttt ttaataagga atactgcgcc agctaatcgt
    16081 ttatctggtt tatttgtctc gattttgaca aattgcttac tccctgtata tacagagact
    16141 ttctttgtac tgacaccaaa accaaaatcc agtgttccgt tgttgttgat ttccttgttc
    16201 gcctgtgctg tactgcttag ctgcatctga taagaaatgg tcagtttttt tccagcatat
    16261 ttttccaacg aagggatcga aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca
    16321 aagccttctg tcagcttttc tccattcgat gaaatcgtta aactattagg taaaaacgtt
    16381 aatgcttcat ccgcactatc tgacaattcg aacttttgat agtcaaggat atttacagga
    16441 attgtcgtat gcaaggaata agtgattttt tcaccaatcg taaaatcatt tctaggctct
    16501 tctaatacct tctcaaaagg tggatcaggg tagtcgtttt cttcattttt aggatacaaa
    16561 tggatagttg aaagtttttg attgttttgt ccatgtacag gaagaacaac taccatattc
    16621 tctgcctttt ctttgacgac ttctggtact ttggattcaa tgaaaagata cgctttatct
    16681 cttttttctt gatctttagc ggccagagaa aaagaagcga taccatcttc gttgttaaga
    16741 gttgtagttg ttgcttctgc ggtaaacata ccggaagacg caccgttttt tgcgatctct
    16801 gcttgtgctt cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca
    16861 tcataaactt ggaatgtgac accatttaat cctcgatacg tttgtaataa agctttttct
    16921 tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa
    16981 gtaatctgaa ccatttctgt ttctgtttct gttgctacct gagtaaaagc tggtttaaat
    17041 agtaacaaga agagacacat actaagccaa ccaagttttt tcatatttag ctgccccctc
    17101 tttctttttt caaaaaaaga taccaaatag aaaacgcaca accaatcatc cctactagat
    17161 agattcctaa acggccaatt cctcccgttt caggtaatgg tacttttgcc tgattcgtga
    17221 tgtctaaaga aatctggttg tttttggctc ctggtgacag aacagattca tgatcttgtc
    17281 catccacttg aattgaccca tcttcgtgta taactatagt aactggctct tttagacctt
    17341 ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt aaattttcga
    17401 atagaaaggt atctgtttct tgtccatctt ttggcgattc taagtccatt tctggtccct
    17461 gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta
    17521 gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca tacattattt gtcatctcgg
    17581 tattttttcc cgtgtaaatg atttttccat atttatcgat ttggaattca taatttcccg
    17641 gcgaagtatc atatccggca ggaccagctg tttcaacgac tcgatattta ccaggtgtga
    17701 gtgaatcaaa actggcaaga cctttttcat tcgttgtttg gctgtcaatt ggctgataag
    17761 taccatttgc ttcttttttc tgtagggaaa atgctgctcc tttaagattt atctctttgt
    17821 ccgtcccttg catcgcatac ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa
    17881 tagtcaactg gatcgtatca tcggaagtag tgactgtttt tccatcaatg gttaccgtac
    17941 catcagaagc gatcttgatt tcccaagtag tctttttgct taatccatgc ccttctggag
    18001 cttttgtttc tgtcagcgta taggtcattt ctttttgcaa tttgacattt tctggaagag
    18061 aataggttcc gtcgccatga tctgttagta aagtatcaat agaatctcct gttaatttga
    18121 aaacagcgcc aataagatcc ttttcacctg tagaggaatt tttcgttatt ttcaagttta
    18181 acggtgtgaa ttgtttagta tttttccatg tcattgcatc tatttgttga gaatcgtatc
    18241 ctggtacagg taattctttg attgtttggt aactgaatgc ttgaccttga ttattgtatt
    18301 gaggtagtga taatatctct tgataacttt ctccgctatt tgcagataat ttgtcaatgt
    18361 ctgcacgttc ccacgtattt gttgtatctt tagctggttt aatgattcga atatatccgt
    18421 ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg
    18481 gacgatcagc tagattgttg tcaaattctt cccataactt tttgatgtga agactgactc
    18541 cgggagcttt agcagatggt ataccgaatt cagctaattc attggtatca atgtttggct
    18601 ggaaagttgt cctgccgttc atttgatacc agaaatttgg atggaagtcc tcattttctg
    18661 tttggattct cacttggtaa tggatttgga tttcttggtc ttttcctaaa tagatctgat
    18721 tgctcttgat ggtatttcct tctatggaaa tagatggagt gaccgttgta ggacttgtcc
    18781 ccacactctt gactgaaagt gtaccaggct gataaatgaa tggttctgcg attggatcat
    18841 ttatttgtcc attgcttaca gttgctgaga tctgtacagc ttttttggct aggtattcag
    18901 agatatctgt tgcatgatca gctgattcat agtaaagatc gcctttttca tctgatgaaa
    18961 ccatttgtcg catacgagac tctacttctg cttttgagag accagctgcc ggatcgcttt
    19021 gaagttggat gccaagacca tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc
    19081 cgatggttgc gataaacgta ctgtcgattc ttctggatag attgttttgg tcaggtgcat
    19141 catagattct tgagattaga gaagtatttc ccggccgatc ttgcgtatta gaaaactgag
    19201 ttccgtaata attgctgctt gattgtgcgt gtacccgctg tactttatag gaaaatgttg
    19261 gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc atttggaacg gatagcatgc
    19321 ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa
    19381 tggattttac ttgatttttc actgaatcaa atgaacccat ctgtactgca ccgttactgt
    19441 agctatatcc ttcgcttgag tagccgacat atcccatatt gattttgtct gtgataccgc
    19501 tatctgctaa agtatcgaca aaacgatcga caccaatctt tacttcaccg atccgattat
    19561 tgtcgttcat acttcctgac cagtctacga ccaatacgag atcaagaggc gtgatatctt
    19621 tttgtacatt tccacgagca ttcaaataaa catcaaacaa tccgggtgta cttgtttcct
    19681 ttgcatactt tcgtatcgca tagtctgctt gattaccggt tcctccatat tcgatatacg
    19741 aatgggtccg atcattagga tctccattcc aagaatttat accatcccat tgatttgttc
    19801 cgtttttatt gccttgatga tcaagaacat ttgtatttcc tgtaggttgc caactggctt
    19861 taggataagt gccctgatca tctgttgtat attctgtagg aatcgtttga ttagatggca
    19921 cactcacagc tggcaaggat aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt
    19981 cttttgatga ttcttcttct atttcgtctg gctttggtac tgacacatct gtatctttct
    20041 tattttctgt ctcatcaata atgtcatatt ctttattatc gacgatcagc ttgtttgcct
    20101 ggctgtggta aagtgctgga tcgattggaa aactcagatc gatagtttgt ttagtgtttg
    20161 atgagatttc caacgtaatt ttcccgctat ttacctgata agcatagtca tctccttctt
    20221 ttctttccac atcacttaag taattttcga ctagaccaat ttctttttct atcttttcag
    20281 cactggtgtt attcgcattt agcttgatgt tcagtgcttt ttttccagac tgatccgaaa
    20341 tgacttcagc tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct gctattgcag
    20401 ataatggcac aaagtttgat aaactcaata ttatcagcat gaaaataact ttcagtttct
    20461 tccctgtggt cgtcattttt cccctccttt ttcttttttt ctgttttgac gtgaatagtg
    20521 aaaattatta aaataaacag gcgttacata attttattta ttttaatatg ataattatta
    20581 tacgaagggc gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag
    20641 ttgattcctt cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata
    20701 tgatcaggtt gttggaatta aactatagaa agatggaaaa aatcattcga attctgtcca
    20761 tttctgacaa gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta
    20821 catttgtaag gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat
    20881 cttcacaaaa attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc
    20941 tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc
    21001 cgggtaaaac gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac
    21061 gtaagttgaa acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc
    21121 aaggttttca gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt
    21181 tctttttaag ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca
    21241 aagaaattat ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg
    21301 agcaatcttt ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc
    21361 catttgaatg gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact
    21421 tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga
    21481 actattttgt caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg
    21541 agcagatacg aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa
    21601 acgaagaata tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat
    21661 acgatacgac tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc
    21721 agatacgtct atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc
    21781 agaatctgat ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa
    21841 ttcaccatta tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata
    21901 gtcaatatat tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga
    21961 caactgctat tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg
    22021 ttacgaatca acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt
    22081 cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc
    22141 tatgtataaa ccaccctgat aaaaatttca ggacaaatac acatagctgt ttctttttat
    22201 tgtattcctt cagcaatcca acgagattta tagcgaaagt cttttaacaa ctcttctttt
    22261 gtccaattat ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat
    22321 aggaaactaa ctgcttcttc cgacgattga atggagaaat gattgattct taataattcc
    22381 agccattttt cgttcattgg agaatcagcc gatagttctc tacgtttggt tgtctgctga
    22441 tcatttcgct gggttaacaa gtataacctt tgccaataga tactttcgtg atctaagtcg
    22501 cagcaccact ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg
    22561 aattttaaga tatcttgatg gactatgtga gaactgcttt tgatcgtata caaataagca
    22621 tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg acatccccat cttgtcaacg
    22681 atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa atatctccag taataggtca
    22741 tcgattctct gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg catccacatc
    22801 tctcctttat ctctgattat acgtggatag cccttcattg tcatgaagga ttcattcctt
    22861 ctttttctct tcttctgtca agtacccatc ttccattcga taaacgcgat cactccattg
    22921 aatcatacgg acatcatgag taaccataat agtcgctttt tgtttctcat gagcttcttt
    22981 aactagtaat ttgacgactt catatgcatg atctgtatcc aaactagctg ttggttcgtc
    23041 tgctaagatc aagcttggct cattatataa agcgcgagca attgccactc tctgtctttc
    23101 tccaccagaa aggtctttag gataactatt tttaagtgac tggatatcta aagattgcag
    23161 caaggaatcg atttttgttg aatcttcttt ttttgaagta acttggtcaa ctagttcaaa
    23221 ttgttccttt accgttaaaa aaggaatcaa attagagctt tggagaataa atcctatttc
    23281 tttaaagcgt aaatctgctc gttttttttc tggaagctca gttagtgagt gaccgttgat
    23341 cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga aagtactttt
    23401 acctgaacca gaaggaccaa tgatgctgac gaattctcct tcctttacat aaaaatcaat
    23461 tgctttcaaa gctgagacct ttgtatgacc agaaccaaat gttttggtaa cttttttcat
    23521 cgttaatatt tttttcattt ttattaacct cctattgctg taattgggtc tactttggtt
    23581 atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat cccactatat
    23641 agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc
    23701 aaataagcaa acaaaagagc aagcagaacg cctaccaccc caacaataaa ggactgtgct
    23761 actaaagatt tagcgatgaa gaaatttttg atcccttgtg ctttcattac tccaaaaata
    23821 gcagtcttct gaagtgtcat cacgtacatg aaaataccga ccacggcagc aaccactaag
    23881 aacaaaaagt atatcatcgc atttaaggtc atattttggg cagaatagcc aggaatactt
    23941 tcaatgaatg tcccaatagt taataactgt agacccttcg atgctttttc tttcgaaatt
    24001 ttagctgaat cctttattac gatcccattg atcggctgtt cttcttcaga agcaaacggc
    24061 tgttgtccat acttcaaagc agtccacgta tctagggacg catagatgac gggagaaact
    24121 gtgtaataag tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta actgccaatt
    24181 ttgatcgtat ctccaatttt atagcctgaa tcagccagat tttgagagat gatgatttca
    24241 ttctttgttt taaattcatg ccctttcgtg agttttggta ataaaaatgc ttctttcgtt
    24301 gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga ttgctccgct gtacaatccg
    24361 attggtgctt tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct
    24421 gcaaatactt gatttgcatc ctccgacaaa acaatctctt gtgcgtccca atcatcgatt
    24481 gcttttttaa actcttctga aagtccattc gctagtcctg acaacatgaa tacgacatac
    24541 gcaatcaaga acataatacc gacgatcaag ccatatcgta atttagaata gcgaatttct
    24601 ttgatagcta gaaacatttg tttttcctcc ctatgataga ttgatagtga caaaatgtca
    24661 cttgttgaat agtgtaactc taaagtgaca atttgtcact tgtgttgttt ttttcttatc
    24721 aataaattgt ctaagacatc tttcgataaa accttaacat gagtcataaa aaatttattt
    24781 aagccttatt tatgctttcc atgataaact aaagtgacca aatcaatgag gtgggaaaat
    24841 gattaaacta ttagtagtag aagacgataa agtattatcc aataatataa aagaaatact
    24901 cgctgcaatc ggcgagataa cacaagttta tgatggtagt gaaggactgt tcgagttgga
    24961 aagtggagtt tatgatcttg caatcttgga tttgatgatg ccggaaatga atggatatga
    25021 agtgttagca aaaatacgga aagaaaaaat acagactcca gtattgattt tgactgcaaa
    25081 agatagtttg gaagataaga ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa
    25141 acctttctat cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg
    25201 tctctttgat gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa aaagcaatga
    25261 agtcttgtat aaagaacaag ttttgcccat acaaggaaaa gagtttgaat tgctcgttta
    25321 ttttttacaa aataagggaa tcattttgac aaaagagcag atatttgacc ggatctgggg
    25381 atttgattct gatacgacga ttacagttat tgaagtttat atgagcaact tacgaaaaca
    25441 tctgcgaagt actggattgg ataaagaaat caaaacctta cgtaatgtcg gttatatact
    25501 gcaaagggtg tgatgcaatg aatggagata ttacacgaaa gcaacgtagt cgcttttttg
    25561 cgacaaatgt agcagcattt gcttttatct tttttctttt gggattcatt actttacaga
    25621 tattaaacca atctgcatac agagaaacag atctgtcatt agagaaaata aattttgatt
    25681 ctcgaacagt acaattagag atacaaagat atcagcaagg cgatcccttt ttaaacccga
    25741 ctcaaacgga acaaaacaga acaaacatgg aacagccatt aatggaacca tcaaatgacc
    25801 gttttaacac tcaagtgatc ttgtggtcta aaaatggtga gatattgaat aaagcagcta
    25861 ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat ctgaatacca
    25921 tccaaaatat tgaattaaat gaagacagtg gctcatctgc cctctctttc cgttcgatta
    25981 cgaaaaaagc ggagacgaat caagatggcg ttgcctacat ccaaatttta gaaaatacaa
    26041 atcaaatcaa gaattcatta gcgacttttc gcacgattat gattttgtgt atgattgttt
    26101 tttggattct ctctattggt atcagttatt acttgtctag tctaagcatg cgccctatta
    26161 ttctttcttg gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac
    26221 cgttaacgat tattcaaaat agtttggaac atctttttac caagcctaat catactatta
    26281 ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc actggattga
    26341 cttcggattt gctgacaatc gctcggaatg attctgatca gcagctgctt tcaaagcaaa
    26401 tgatcaatac acaagaatat atcgaacaac ttgttaaacc atttcaagag atggccatca
    26461 tagatggaaa agagtttatt ctccataacg atgcaacatt agaagtacca attgatgaaa
    26521 aaaaaatcca tcaagttttg gttattttgc tggataatgc attaaaatat acgaatccag
    26581 aagataagat cattttggat tcatctgtta accataaaga atggctgatt cacgtaaaga
    26641 atactggccc ttctatcagt gaagaagatc aagcaaggat ttttgatcga ttttatcggg
    26701 aagatcaatc tcgggcgaaa gagactggcg gatacgggct tggcttggca attgcaaaac
    26761 aggtaatcga acagcatcat ggacagattt ctgtatccaa ttatcttcca caaggagtcg
    26821 attttaaagt ccaattgccg ttgaagccta aaaaataatg cttatcccta aaaaagggct
    26881 gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata aacggtgttc cagaagtagc
    26941 ttcttcggaa ataagccgaa atttctttaa gctgaccact tctgtcacaa cctctttagc
    27001 ttacgcttac gattttttat tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc
    27061 caattcttct cggctaacat cttctgtcac tttttcaaca tcttcatgtt cttctttatg
    27121 aatcccaact tcttcacgaa taactgtatg tttgcttaca tctacttgct cttctttgat
    27181 tgggatagta atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag attgatctgt
    27241 tactggtttg cgttcgatga cgatttcttc acgtttgacc ggtacttcta ccgtttcagt
    27301 atcgttgacc acatgtttgt ggatgtcgac ttctccagtt gtcacatcat gtgtatttac
    27361 atctaatttt tcttctttta gttgaattgt atcatcatct agatttggtt tgcgatcgat
    27421 gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca tcggatcagt
    27481 tcctggtgtc atattcaatg gatccgttgt attttgaaag cctgtcgtgc cacctacgtc
    27541 cattggatct gttccagtgc cagtttctaa tccagtcccc attgtgcttg ctgggctaac
    27601 agtatcttgt gtatccattg tatcttctgt tgtttctggc tgacgatagc cttttactgt
    27661 gatgacaaat tttccatcag aaatatctcc tctatattga tataaaggat cattttctgg
    27721 tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta tcttttattt tttcccataa
    27781 agaacggtct tcttcaccat ccatccgatt ttcatctgtt tcaacatcta taccagacaa
    27841 tccgtcaaat cctagtctag atgctgcttc atcagtagta taaataacga tatcatctcg
    27901 ttcataccct tcgttacgca accgttcaat tactgataat gcttcttccc tcgtactata
    27961 gcttccgatg accgttaaat ttttctcttc catgtaaatt cctcctagca aatagattta
    28021 tgattggtat attgttactt ataaatttat tctataggag acgattgttt tctacaaata
    28081 tttagattgg aatttgatta aaacaattta attgaaattt gaaaacattt atgttaatga
    28141 aagtcatatt ctaataaaat gtcatttgtt tctatatgaa tttttatgaa aaatttcata
    28201 taatttccac tcgttgtgat ggtttaattt atccaatcca tcaataagca aacaacttat
    28261 ataaaaaagg attaattctc aatataattg ctcctaccat agaataaatc ttcatgtaaa
    28321 gtaatgaagt accaactatt tctaatactt ctttaagaat tatcccaaca atatcgttag
    28381 acaaaccatt attgaataaa tggagagcca atcgattctg aaaaaatgtt ttctaatttt
    28441 tcggaaataa aagaaagagc ctcctgccat tagaacgaac caaatccatt tcattgctca
    28501 tgaagtaacc acatagctaa aaagaggata aatcaattaa aagatcaaaa gaatgactgc
    28561 actaataaaa taactaaaaa tgtatcttct tacttcatat cttttagcta gtacttagtt
    28621 aaaaatttgc ttcctaagat caagacaaac tgtctatctt catttgatga aatgcggtaa
    28681 aatatagaaa atgaacacta ttggagggaa tttgtaatgg acagcaaaac tagaaaacaa
    28741 ctgcaagaaa agttaaagca acgtacacac ttgctccctt ccgtctttca acagttttta
    28801 gaagaaaatc gttcttccct ttcattaggc agtcgctatg aatacgcaaa agattttcat
    28861 ctgtttactg attatttgag aaaagtattt tctgaagaag tggatgatca gctgattatc
    28921 catttagaaa aacaagatta tcagaatttc ttagctaaaa tctatcgaca tactcgttcg
    28981 ttccaaacta cagcgaacca agaaacggag caattgtttg aaaatagcta ttacgggatt
    29041 tcaaggaagc aatattcatt aaatcatttt ttacgatttt tgtatcaaaa aggactgatt
    29101 gaagaagaga tttctctttt gggtacaagt ttaccggaaa caaccaaagc agctccgcga
    29161 tatatcgatg cagaactttt caaagatttt gagcatttgt ttatcccgat tgaaggcttt
    29221 caaacaagca gagaagctag ctttgaagaa aagaatagac cgaggaacct tgcaattact
    29281 gccattcttc tgtattgcgg attaaagatc catgaagtag tagaacttaa acgtaaagat
    29341 gtatctttga ccaagcagat tcttaatctg aatagaaaag aaaacagact gaataaagta
    29401 ccaataccaa aagaagcgat gccatttctg gagaactatt ttaagttaac agcacaaaat
    29461 acggacgaaa attcttttgt ttttcgttcc tcacatgatc agcaaatttc tcctcggact
    29521 attcgccaaa ttttgagcaa aattacagta tataaaaacg tatcgccaga actatgcaga
    29581 aaaacatatc gttattatgc agaactatat ttagatgatg ctgacgcggt aaattattta
    29641 tgtggtaatc gatacctctc atcccattct tttcaagata tctgggaaaa gatggttgat
    29701 ttttcttatc acgaattagc ggcattcgtt cgggtttctt ccatataaaa caaatgtatg
    29761 atcttttttt cacaataaac tatgttatta tataaaggat tatgttacga ttatcttgaa
    29821 tttgtatatt acttaacaaa agataatgga gtgtgaatga tggagaccat aacgtattcc
    29881 cctttgaact tatttacgaa ttttcaagaa gctgcagcta acttttccga gaccccgatt
    29941 atctttgatg agccactttc agctttccca gaattaggac ttgaaacaac ttacaaaaaa
    30001 tgctgcgaaa gtgtgttgaa acgggcttat caattggctc atcttggagt gaaatccgga
    30061 gacaaagtta ttatttataa gagttccgct tttgacacat accttctagc tgtttctgtg
    30121 tcttaccttg gtgcagtgcc tgtcatgact tcttatcatc taccaacaac aacaatggaa
    30181 gtatttatcg atcgtttaga agaccctttc attttatttg atgatgagac aaaaaagcga
    30241 gtgagagaaa tttcaaatgg aacaaaatca aaacagattc ctattcttta tttaatggaa
    30301 caacctgctt tgtctgtttc tcaatcattt ttagacaaaa atgaaatttc ttatatgacc
    30361 catacttctg gtacaactgg tatccctaaa cttatttgtc attctgctca ttctatggga
    30421 tggcggacga aatggcaaaa aacagttttc acaaaaattt cggaaaagaa attaatcgct
    30481 tttcacatct ctccagtgca ttccagattc aatattggta tctcttcttt gatgtccatg
    30541 ggatttccta tgatgccttt agcaaacgca caaagttcaa aagttgttca tatgttggaa
    30601 gctcaccggc caattgcact agagacccat ccgaataact ttgttcagtg gcgttttact
    30661 gctaaggagc atccagaagc tttttcgggt atccgatact atcactccac ttttgatgct
    30721 ataaacaacc agacaatgaa gacatttttg cgaacgtcga tggcacagga tgcggtattt
    30781 cttcaagtat atggccaaag tgaatgcgga ccaatgattc ttaaggctca tacactagag
    30841 agtttgaaaa caagtgatgc aagagatatg ggcgttggac tggaagacat gacaagcgct
    30901 cggattacag attcagttgg gaatgtccta cctgaaaaca cagatggtca tatacaatta
    30961 ctgtctaaag gacgggcttt gacttattac aaggaagatg cacggttcca ggaaaatgtt
    31021 tatggcgatt ggtgggatag cggagattac ggttttatgg ataagcatgg ccacctattt
    31081 ttgaaagatc ggcaagtgga tttgatcgaa acgatcaata gtacattagc tattgaagac
    31141 tttttattag attcgttaga ttttttggca gaagtagtga ttgtaaggga taaaaatcat
    31201 tctccacaac cgattattgc tttagctcct gataaagaaa tggattggaa tcgatggtgg
    31261 gagcaagtcc atgaacttcc tcgcttgaac gtccctatca tccgtgattt tgatgcaatc
    31321 ccgcgaacag ctacaatgaa agtgcaacgg cttcaaatcg agaaagaatt aaaatcacaa
    31381 aagtcctaac tatttaagga aagaaagatc cattttggtc tttcttttct ttttgttttt
    31441 ttaaattgga tgagataaaa atatgccaat tggctgtttt tcttttttga aaatttcgtt
    31501 atactcttat tcatggagga aaatgttatg aaaagaatct tgacaattgg cggttctgat
    31561 ccatttgctg gtggaggaat ccaaacagat ttaaaaactt ttgagaattt tggtctattc
    31621 ggactaagcg ccttgacttc tataggggca ctagatatta atgagcgatt tatgctggaa
    31681 tctatatctt tggaattgct taaacgacag ttagtttcga ttgataaaat gacttcatta
    31741 gatggtatca aaattgggtt attgaattca agtgaagcaa tcatggttgt tcgagacttc
    31801 ataaaaacaa aacaaaatat cccaatcatt ttagaccctg ttttggcatt caaagaaact
    31861 gaagctgtga tcaatcaatt gtatatttcg cagctcatcg aagaactttt tccacttgtt
    31921 acgatgatta cgcctaattt ggcagaagct gctttacttt ctggtcaagc tcttccctct
    31981 tcgttaacag aaatgatcca tctcgctaaa gaactcactg cttcaggaac aaaaaacatt
    32041 gtgatcaaag gcggcacaag gatggctgga gatgaagctg tggatcttct ctattcaatt
    32101 gatcattatg aggtattttc tcataaaaaa atcgacacta caacagtaaa cggggccggt
    32161 tgtgcctttt cttctgcaat aacagcgaat cttgtgttag gggcagattt taccaaagca
    32221 gtagcagaaa gtaaaaaatt tgttgcccat tgtatttcta atggcgtgct gatgaacgat
    32281 cataccggaa gtgtctggtc tggcggtatt caaaaaggag gaatgagaaa tgaaaactaa
    32341 gcaattaacg atctatgcta tgctgattgc gttgacagtt gccctttcat taacaatttt
    32401 gatccctgta ccggctacga atggatttgt gacattatgc gaggccggga tctataccgc
    32461 tgcttttctt tatggtccaa cgggtggatt agttgttggt gcttccagtg gtctattgat
    32521 cgatttgatt tcaggatatc cgcagtgggc agttttttca ttagtgatcc acgggttaca
    32581 aggatatgtt gctggttctt tcagcaaatc ttctactcgt atgtggggat tagggctttt
    32641 attaggaagc ttagttatga tcatcggcta tggtatcgcc ggttggattt tatatgattg
    32701 gccggctgga gtggcttcta taccaggaaa catcttccaa aatctgattg gaatgggact
    32761 tgcctttcct ataactgcaa gtcttcaacg tattcaagca cggaagcaat attaattgaa
    32821 aaagaaggtt ttagaaaatg aagttgtcag aaaaagaatt aaaagaccaa ttgactgaaa
    32881 tagttaatga catattggct gaggcacact tgaaaaaagg tgatcttttt gttttaggat
    32941 gcagcacaag cgaagtcgtt ggaggacata ttggaaaaaa ttccagtgcg gaagttggtc
    33001 aatggatcat ccgtacgtta aaagagttgt tggatcctaa agaaattgcg ctagctgtcc
    33061 aaggttgtga acatttgaac cgagcattag tagtagaacg aacagtggca gaagcaaaaa
    33121 atttcgaaat tgtctctgtt gttcctgcac tacatgctgg aggtgcttgt tctgttgctg
    33181 cttttgacca gttcaatgat cctgtcgaag tggaacacgt agttggacaa gccggtatcg
    33241 atatcggtga cacatcaatc ggtatgcata tcaagcatgt ccaagtacct gtcagacctc
    33301 gtttgaaaac ccttggacaa gctcacgtga cagcccttcg ctctcgtcct aaatatatcg
    33361 gtggtcctag agcgaattat tgaaatgagt aattgacaga gcaatctgaa aaacttcctc
    33421 gcaaagtcca actaatgaac tttaacgagg aagttttact tgtttgtttt atataaccga
    33481 gcttcataag gtgctagtat gcctttagct tcaaattctc cgctagagga atttgacaag
    33541 agtaatgtcc aggactgatc tgcttctttt ggaagttcat agtcagcaag tgattctgtt
    33601 aagtttacca taataagata ttgttcatct tctaaatatc tttcatagac ataaagttgt
    33661 ggatgttcat gaagatattc tttataagaa ccgtatatca gacctttatt ggtttgtcta
    33721 atgcggatca tgttcttata gaattgcagg accgactctg gttcatattc ttgttgttct
    33781 acattgatcg ttttggtatt tggattagtg atcagccaag gttcatgtgt agaaaaacca
    33841 gcatattgtt ccgaagtcca ttgcatgggt gtcctgctgt tgtctctcgt tgtttcacga
    33901 atgatatcca atgcttcttc tcttgtctta ccttccgcaa gcagcctttt ataaagtctt
    33961 ttcgaatcta ctgcatcaac ttgatcgatc gattcaaagg gcatattcgt cattccgatt
    34021 tcctgtcctt gataaataaa tggcgtacct tgcaaaaaga aatacatcat agcgattgct
    34081 tttgcagaca tagcccaaaa atcgggacga gtatctccaa acactgaaac ggcacgagga
    34141 acatcatggt tttccatata taaagcgttc catccgatac catccaatga agtttgccaa
    34201 gcgcttaatg catgtttcaa tttaaggaca tctaattgcc cttctttttc ttgctgccag
    34261 atatggatat gatcaaattc gaatatcata tcgaaataac cgtctttccc tacccattgt
    34321 ggtccttctt ctgcagtgac accacttgct tctccgacag tcatgatatc gtactcttca
    34381 aaaacatgtt tcaaatcggt caaatgttct tcgattcctg agacattttg aaagggtgag
    34441 aaagggttct ctgtggcttt tactgaatat tcatcttttt ttacatgaga aatcgcatct
    34501 aaacgaaatc catctatccc tttatccaac caccatcgga tcatattgaa tagttcttct
    34561 ttcaatttct cgctttccca atttaagtct ggttgttctt ttgcaaaaac atgcagataa
    34621 tactgtccag tttcatgaga atactcccaa gcagaaccgc cgaaaatcga tgtccaatta
    34681 ttcggttctt tgccatctac cccgtcgacc caaatgtaat aatcgcgata cgggctttct
    34741 ttagattttt tcgattcttc aaaccattga tgctgatccg aactatggtt gatgacaaga
    34801 tccataatca ctttgatccc taattcatgt gcttctttta gcaataaatc aaaatcttcc
    34861 atcgttccga atctctcttc tatatcttga tagtcactta cgtcataacc attatcgaca
    34921 aatggagatt gatagatcgg attgatccag ataaagccga taccaagatc tttcaaataa
    34981 ttaagttttt cacggatccc attgatgtct ccttgaccat cgccattgct atctttgaaa
    35041 ctagcaggat agatttgata gcctactgct tctttccacc atttttgttc ttgtatcata
    35101 tcgtttcact cctttaacgg tccctcttga ttataaccga ttctatttta gaagtatcct
    35161 tgttaccggt aactagaaac gaaaagctca acgaatctgc taagtgcgtt cgttgagctt
    35221 tattttatca tagatagcgg gcaatcagtt gaatcatttg cttgggaaat tcattcaagt
    35281 cagtaatatc tacaaaacga ctttcaccgt agatcgactt gatttcttct ttgtcttggc
    35341 caatagctgc ggagacaaat aggattcctt gacgttcata ttctttcaaa acagactgga
    35401 tatcttcttt tgcttttttg cctgtataat ccggcaacgc ttttggctga ccgtcactaa
    35461 tattaagcaa cagtttggtt gttgcggatt gtctgtttaa tttttctgct gctaaacgaa
    35521 gtgcagcacc atcacgattg ttttgccttg gtttcatagt gactaatttt tcatttaacc
    35581 attgaaatcc gtcttcaaat tctttataag aaaacaaaga agtcttttct cttgacgaac
    35641 ggtctgctgt atctccgtaa atcaacaatg ggatatcgac tttcttagaa aattcggcaa
    35701 tggcaatcgc tgcttttttt gccgcttcga tccgatcatc ccgtatcatt gacccagatt
    35761 catcgatccg tacagctacc gctaaagaag gttgctcatg tggcgggttc tttttgtcaa
    35821 aatttctcaa atcaccatat gctactttgc ttgcattgaa ccgctgacct tcgtattttc
    35881 ctttttggta cgtctctgtt tgttcattat ccagtaactc taaaatttgc cggctaagtg
    35941 attctactat tggcattact tgcatgtgta gctcacgtgc ttccggcgtt tttccttgga
    36001 aagctggtcg gtgaacgatg agaccttctt tttcatgaat cgtttccttc atgatttgtc
    36061 gcgcttcttt attcagttgc ttcttgattt ccttatcatg tgcttccatg tcagaagcag
    36121 acatttcttg agaatcatct cctgtttgtt gttctttttg attttttcct tcgatatcgc
    36181 tactgcgttc tttttgcatt tgaggaggaa tctcgttttt ttctattgtt tcttcttgtt
    36241 tctcttctcc ctcatttggt tcttcagcag acaacgattt cttttcatct aagcgcagaa
    36301 tttcttcagc attttcagtg atgtttcgtt catttgcttc ttcggcgcta atggttcttt
    36361 cttttgtatg ctcagattga ggatgacccc atttttcttt tctcaatatg tcctgaagtt
    36421 ccagtagcaa tccactgtca gacaaagctt gttcaagaat tgccaattca tctgaatcag
    36481 tggataattt atacaaaact tttggataaa tcgactggag taaatcttgt ccttgcttat
    36541 aggtatttgc ccaataaaaa taagaacgca ttcctgctac acctttgatg gcgttagctt
    36601 ttgcagtttc atccaacaaa cggataatct ctgccatctt taagagaagt tcttgttttt
    36661 ggatcatagt tttcgccatc gcacgttctg ccatgacttc taaggcagga agatccattt
    36721 tttccgcatg ttgcatccga tctcttaaag attcatttaa tgggcgattt ccttggtaat
    36781 ttcgatttgt agtgatcaca atgacgcaat ctggatggcg acgaatgatt cctgtcggta
    36841 aattcaacat cccatttggt tcgagcgctg agttaagtgc cacaagaaca gaggcatctc
    36901 ggatgacagt cggctcttga atctctaata aatagccttt ttcaatcgct cgaacgattt
    36961 cagaagggta atagctgtaa tggattgcat ctggataatc agcttcgatc ctttgaacta
    37021 gctctgctaa tttttcttta gcagaaaaag gatcaatgcc gtaatggttc tggatcagtg
    37081 tcagtacagc ttcaagtgta tctgtttggt aaatagcttc tagtaattct tggtcttgtt
    37141 ccttattaga atctactaca ggtaataagg ctccgaaaac atctgatttg tccatatctg
    37201 caaaacaggt cacttttgta tagggaagtt gcagatcagc agatagtgct ttggctaatt
    37261 gtgtcttccc cgacccggca tctccttcca aaagtacgtt actgattttc atttcaggat
    37321 cttgccaatt ccctttgatt tcggaggcga tccgccattc ttctgcactt gtccgatggc
    37381 ttttcggttt cttccagatc atttgctttt ctagccgtga aaaggttcgg ttttctgtta
    37441 gtgagaaatt ctccatgctt ctcttcccct tttgatcatc tctgtctatt aatatcctag
    37501 ctcatctaaa agacgtttta aaatacggaa tcgttcccca atcagttctc cgtctttttg
    37561 tagtgtatcg taatacaaaa tgatgtcttc atcgatcttt ggatttttgg tatctacttc
    37621 taccgtcatt cggttccctt gtaatccaat gatgtctacg actggatttt ctggatcata
    37681 aaatttttca taacgataag catcttggaa gtataaactt ccttgcgcag ctaagatcgc
    37741 taaatctaaa atacgtgtga ttggtaattc ttccgactgt cgtgaccatt tttctccagt
    37801 atgtcgccat accttgcctg agatatcgac ttttccacga tcattccact gagcaaggcc
    37861 cagtgaaagc ccttttgcat ccatatttaa tgcatttctt ccatcaactt tatcatagtc
    37921 ttctacaacg attactggtt tatgttttaa atttgttggt atttccatta ttttaactcc
    37981 tcatcctttt atttttagta aataagtaaa ttagtagttt actcatttac taaactatat
    38041 gcgacattat agaggattga agtgaggatt gcaaagaaaa agagcttcat tgatagaaat
    38101 aaaaacacca tctcgttctt ttacacgctc attagtaaca tgtagaaagt ccaagatgat
    38161 gttggaatat tttggctcta tactttttga tgttggtgaa cttcaccaac atcttttttg
    38221 ta
//
LOCUS       DASHFF010000022        37148 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.022, whole genome shotgun sequence.
ACCESSION   DASHFF010000022 DASHFF010000000
VERSION     DASHFF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.022"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..420
                     /locus_tag="U2I75_001252"
     CDS             <1..420
                     /locus_tag="U2I75_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN1696399.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="U2I75_001253"
     CDS             complement(504..1226)
                     /locus_tag="U2I75_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1696400.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="U2I75_001254"
     CDS             complement(1216..1953)
                     /locus_tag="U2I75_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1696401.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="U2I75_001255"
     CDS             complement(1965..2957)
                     /locus_tag="U2I75_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1696402.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="U2I75_001256"
     CDS             complement(2972..3715)
                     /locus_tag="U2I75_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696403.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="U2I75_001257"
     CDS             complement(3956..5278)
                     /locus_tag="U2I75_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1696404.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="U2I75_001258"
     CDS             complement(5265..6230)
                     /locus_tag="U2I75_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1696405.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="U2I75_001259"
     CDS             complement(6227..7765)
                     /locus_tag="U2I75_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN1696406.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="U2I75_001260"
     CDS             complement(8181..8468)
                     /locus_tag="U2I75_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696407.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="U2I75_001261"
     CDS             8602..9270
                     /locus_tag="U2I75_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1696408.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="U2I75_001262"
     CDS             complement(9561..10301)
                     /locus_tag="U2I75_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696409.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10433..11380)
                     /locus_tag="U2I75_001263"
     CDS             complement(10433..11380)
                     /locus_tag="U2I75_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1696410.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11509..12273)
                     /locus_tag="U2I75_001264"
     CDS             complement(11509..12273)
                     /locus_tag="U2I75_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1696411.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12277..12666)
                     /locus_tag="U2I75_001265"
     CDS             complement(12277..12666)
                     /locus_tag="U2I75_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696412.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12801..13343
                     /locus_tag="U2I75_001266"
     CDS             12801..13343
                     /locus_tag="U2I75_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN1696413.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13491..14123
                     /locus_tag="U2I75_001267"
     CDS             13491..14123
                     /locus_tag="U2I75_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696414.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14257..14382)
                     /locus_tag="U2I75_001268"
     CDS             complement(14257..14382)
                     /locus_tag="U2I75_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1696415.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14516..15409)
                     /locus_tag="U2I75_001269"
     CDS             complement(14516..15409)
                     /locus_tag="U2I75_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN1696416.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15591..16517)
                     /locus_tag="U2I75_001270"
     CDS             complement(15591..16517)
                     /locus_tag="U2I75_001270"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN1696417.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16602..17363)
                     /gene="pflA"
                     /locus_tag="U2I75_001271"
     CDS             complement(16602..17363)
                     /gene="pflA"
                     /locus_tag="U2I75_001271"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN1696418.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17607..19838)
                     /gene="pflB"
                     /locus_tag="U2I75_001272"
     CDS             complement(17607..19838)
                     /gene="pflB"
                     /locus_tag="U2I75_001272"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN1696419.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20115..22565)
                     /gene="parC"
                     /locus_tag="U2I75_001273"
     CDS             complement(20115..22565)
                     /gene="parC"
                     /locus_tag="U2I75_001273"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN1696420.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22577..24622)
                     /gene="parE"
                     /locus_tag="U2I75_001274"
     CDS             complement(22577..24622)
                     /gene="parE"
                     /locus_tag="U2I75_001274"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN1696421.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24788..25222)
                     /locus_tag="U2I75_001275"
     CDS             complement(24788..25222)
                     /locus_tag="U2I75_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN1696422.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25349..25987
                     /gene="plsY"
                     /locus_tag="U2I75_001276"
     CDS             25349..25987
                     /gene="plsY"
                     /locus_tag="U2I75_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN1696423.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26119..26994)
                     /locus_tag="U2I75_001277"
     CDS             complement(26119..26994)
                     /locus_tag="U2I75_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN1696424.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27076..27867)
                     /gene="codY"
                     /locus_tag="U2I75_001278"
     CDS             complement(27076..27867)
                     /gene="codY"
                     /locus_tag="U2I75_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN1696425.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27885..29285)
                     /gene="hslU"
                     /locus_tag="U2I75_001279"
     CDS             complement(27885..29285)
                     /gene="hslU"
                     /locus_tag="U2I75_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN1696426.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29299..29847)
                     /gene="hslV"
                     /locus_tag="U2I75_001280"
     CDS             complement(29299..29847)
                     /gene="hslV"
                     /locus_tag="U2I75_001280"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN1696427.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29884..30789)
                     /gene="xerC"
                     /locus_tag="U2I75_001281"
     CDS             complement(29884..30789)
                     /gene="xerC"
                     /locus_tag="U2I75_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN1696428.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30987..32363)
                     /gene="trmFO"
                     /locus_tag="U2I75_001282"
     CDS             complement(30987..32363)
                     /gene="trmFO"
                     /locus_tag="U2I75_001282"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN1696429.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32332..34410)
                     /gene="topA"
                     /locus_tag="U2I75_001283"
     CDS             complement(32332..34410)
                     /gene="topA"
                     /locus_tag="U2I75_001283"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN1696430.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34532..35389)
                     /gene="dprA"
                     /locus_tag="U2I75_001284"
     CDS             complement(34532..35389)
                     /gene="dprA"
                     /locus_tag="U2I75_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN1696431.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35445..36212)
                     /locus_tag="U2I75_001285"
     CDS             complement(35445..36212)
                     /locus_tag="U2I75_001285"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN1696432.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDVRSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36205..37065)
                     /gene="ylqF"
                     /locus_tag="U2I75_001286"
     CDS             complement(36205..37065)
                     /gene="ylqF"
                     /locus_tag="U2I75_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN1696433.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT        10091 a         7906 c         6368 g        12783 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata tagtaataag acttaattcg
    10441 ttgtctctct gataactagt tcaggaaaaa aacgacgata accttgactc gccccttttt
    10501 cttctatttt ttttaacaac atttctccac atacttcacc catacgtata atatcttgct
    10561 tgatcgttgt caatttaggt gaactgattt ggtctaaaaa taccccatca aatccaatca
    10621 caccaaattc ttcgggcaag tgtcctccag catttataat ccctcttact gctcctagtg
    10681 ctagccgatc tgaactacat acaaaacaag ttttatctgt aaaatctgcc cagttttctg
    10741 tcatatatct ttctacttct gtcgaatgat ttggaaaccg caagatttta ctaggaagat
    10801 caacttcctt catcacagat aaataaccag tttcccttga aatttcaaaa ggctcgtcca
    10861 cttcgatacc gatataaata atcgtttgat aatctctttc taaggcatat tgcgttgctt
    10921 ttgctgtacc atattcatta tcgctgtcta cataatcata tccttcagaa ttttccccaa
    10981 acaagacaac tggtttttcc aaacggtcga tccaatcgaa gtcttttttt ctgacacctg
    11041 tgatgatgta tccatcacaa gaaccgacgt cgaaggcatc atttgtaact aattggagag
    11101 aataatgcgc tttaccgatc cgtttagcga ttcccatcag caaattcatg taatagggct
    11161 cagttgtgtc gatttcttcc agtataaata atttgatgat ctgcgaccga ttcgtgacaa
    11221 gtgcttttgc agcaatattt ggacggtaat ccagttcagc cattgctgag aaaactaact
    11281 ctttcaattc gtcagtcacc tgttccggat gattgatgac tcgagagacc gtcatttttg
    11341 aaacattggc acgttttgca acatctgcta aagtgggcat gctctcttcc tttctaacat
    11401 gttgtcattc ttactatcaa ttataccaaa ttttcctaac agagtttatt atagcccaaa
    11461 ataaaaaacg gaaaatcctc tatcgtcatg agtgattttc cgtttcaatt atttatactt
    11521 ttttatcaaa ttttttgcta aaaaagtgga agcttcttcc attagtggta tttgtaccat
    11581 atgtcgctgt gtttttgagg aacgaaaaac aatttctttt ccgtaagact tatgccgatt
    11641 tttttctaca aatcgttccg tttgtatata agggattttt tcgtcagctt gtccatgcca
    11701 aaagaacaga ggacgtccag ctaacttttc cggctgcaat gacaaatcat agctcttaac
    11761 ccatgaaatc aaatcaaaat agtcttttgg tacgacataa ttatattccg atgcgcgcct
    11821 tacgatttct tttccatatt ccaaaggtgc aggggaaccc atcaaacaca cgcccgctac
    11881 tatatgagga tgtttagcca acaaggcgca tgttgtcatt ccccccatcg aaaccccgcc
    11941 aactgcaagc tgatcatgta gaagacctct tttttctaaa tgcttgataa gtgtgtcaaa
    12001 ttcaaacaga tttccgtaga tactatccca aaatgtatag gatggaatag aagaaaccgg
    12061 ctgtttgcgc tccccatgat tcatcgcatc tggcaggatc acacgaatgc cctgctgcgc
    12121 aatttttctt ccttgcgtca aaactaactc cttacttgtc tgccatccgt gataatagat
    12181 gacaacggga atacgttcct ctcttaattc tttcagaaca acttctaaca ctggaatatg
    12241 atggacagaa cgtttcaata taattggatt caattgttac tcacctattt tcattaaatt
    12301 tcctgatcgc catagcaacc atgctccaac agttgtctgg aacaggttca gtgtaccaaa
    12361 taatataaat gcaggactga atggctcggg aaataagcac agagaacaaa gtcccaccac
    12421 tcctaatgct agcgaaagga gtggataaat agaaaaattc aaaaaactgg caacgctacc
    12481 gaaaatatta ccaataaaca gtatcaaaaa gaaaaaaacg cttaaaatta aaccatctgt
    12541 cagcgacatc tgaaaaacaa tgttctctac ctcaattggc tgttgaatcg aatagacacc
    12601 taagtatata ctgagaagga gacttagcac gtggctggca caaaacacac gattgaccgt
    12661 tttcatttta atcacctaac ctatctttat aattctatta taatcatttt atcatttttt
    12721 tgtatgtata aagtttcaat ttttcttcac ttacgatttt ttttgatatt gttgttatat
    12781 aaatgaaagg agttttacaa atgattgatc caaaaatgtt ggggatgatc ttcctcatta
    12841 attttgctta tatcactctg aacacgttac gtttcatgtt gacaatgaaa ggctatcgac
    12901 tgattgctcc acttgtcagt atggtcgaga tcacgattta tatcgtcggc ttatcccttg
    12961 ttttagatcg tttagataat ccactgaatt tatttgtcta tgcgttaggt tacgcagtag
    13021 gtatcagtgt cggaattttg attgaagaca aattggcatt aggatacatc atggtcacaa
    13081 cgattttgcc agcaaatagc gcagaagata aaagtttgcc gggtatactg agagaacacg
    13141 gttatggtgt gacacagact tctgcaatgg ggctggaagg tgagcggcta gttctcgaaa
    13201 tactttctcc tagaaaaagc gaacgtgaac tgtatcgcct gatcaaagaa atcgaagagc
    13261 gtgcttttat catttcttat gagccaaaat atatttcagg tggtttctgg actaaaaaag
    13321 tgagaaaacg acaaggaaaa taactcacaa acttaaaaac tgtctaatat ctaaattaaa
    13381 ttttaaatgt tagacagttt ttatttatta ttttgattaa aaaacatcga catatactca
    13441 tcaatttgtt attatacttt atatacttat caatttaatt tggagatatt atggaaattc
    13501 aacgtagaac cactaatttt ttacatttag gctctttttt tgcaactttg acgatcttat
    13561 tttttctatg ttattcatac cttaagacct cacatgcgcc ttctagtgca ttaggctggt
    13621 tgcttttgga tcattctgat atcacagaag ccgaagaaaa tccgtttttt ttgattttgt
    13681 cttctctttg cggcttactg ttttctgttt atttcggtgc ctattttttt catcgacatc
    13741 ctggtacgtt aaacgaagct tcaagcgtat tgattgcatt taaaacgttt tcttttttag
    13801 caattctttt ttattcgttt aatcacaata cgcttttctt ctttatcatg aatggtttag
    13861 tcgtgatttt atccattttt acttatttta ttttttcact catttttagc caattgaaat
    13921 gtccgctctt ctttcgatta gttcctgtca cctgttttat ttacagcttg ttttcctttt
    13981 ttgtccttgc tttaatgcca ctgaccaacc cttcgacgat ccaagtcgca ggaaatctgt
    14041 tggatatgct cttcatcgtc agtctaggtg tatttatgtg gcaaagaaaa aacaaacaaa
    14101 ataaaaagca ttttgaagcc tgactattac gacttcataa atgctttttt agttttaaac
    14161 acttcaatct aaaagaataa cccaagattg gataaattat taatgactcg ccattcgttt
    14221 cttagtgatt ctggattatc aaatggactt cctaatctaa tcagctagtt cttcccataa
    14281 aatgccatac ttgactgaat cataatattg attatttaaa aatctaactt ttcttatcac
    14341 tacttccttt gtcattccac atttctcacc atttttttgc attcctttgt ttccagacca
    14401 tgtagtgaat ctaataggtg gtagataggc aaaatactca aataattctt ttaaccataa
    14461 aggaagagaa aggctcctcc tgcaagagtc agtataattt ttgcttactg ctctcttagg
    14521 ttttactcac taaaactgtt ccatattctt tgatataaga aaatgcatgc ggtataaatt
    14581 tcttgctcag ctcagaaaag atttgtcgtt taagaaaggc catttcctcc attgtcaatg
    14641 aaggatccaa tgttggtaat tcaatctcaa aattgttttt ttcgacaata ggtaattcta
    14701 atgtgaatga ttcttcttcc tttatagtat tcgtcacaag tagagactga tggaaaatac
    14761 taggccggcg atttttatat ttattcttac gtatcctgtc aacgataact tctatttgat
    14821 caaaaaagta cgattcattt acgtctccaa taagttcggc taattctttg aatgccttcg
    14881 ttacaggaag ctgattcttc tgtacaaaaa agtaattagc caaagtaagc gggaagtgtt
    14941 ctatagagga aagtgtgata aaacggtact tctcatttcc tgcagttgtc ctcagcaatt
    15001 ctgtctctcg gctgtttctt actccataaa aatacgcttc tttccgttca tcttcgaaac
    15061 aatacgccca aataacttct cccatcgcta atttttgttc atcaacagag agtctcttca
    15121 attgttcagc tatggtttca gcagccgaag tggcttgttg caagtaatct ttaggatcaa
    15181 atattggaaa gttcaaatga tatcttcgtt cttccctctt gattaatcct gcatcgatca
    15241 ataaatcaag aaaatgggaa aatttatttt gcgaaaattc ttttttcaat tctcttagaa
    15301 tcgtttcttg ttctcgatgt tcatatagat aatcggtcaa tgaagaaaaa agcggatcat
    15361 cggtcagttt ttgataaaag ttttccttat tttttgaata atagttcata tatgttctct
    15421 tttctatccg ttttatttta cttaaaaggc ttttacgtaa aaaggaggaa gaacaagagt
    15481 cataattgac tgttattctc ctccatttct tcggccttta gtatgttcta tgtttctttt
    15541 tatatcaatc gcttatccat tcattttgaa tgcttcatac taccatttga ttagctaaat
    15601 acagccgtca attgaggtac gacttgtttt ttacgtgaaa cgacaccttt aagcaaagca
    15661 cggttgttgg tcaaagttgt ttggaaagct tcttctactt tttcttttcc tttaccagca
    15721 acaagtaatt ctgaatcact gtctaagatg tttgtgacaa ctaatacgaa cagatcgtag
    15781 ccatttgctt tcatttcatc ttccattgct gcttctagct ctgcttgtcg atccatcact
    15841 tcattaaggt ccactgtatt gacttgagcg atacgaacgt tggaactgcc cattgggaaa
    15901 ctttttgcat ccaaatcaag aagaaccgca gctgatttat cacttagatt tgttcctgct
    15961 ttcaacattt caagtccata agtgtttaga tcgacttcag cgattgctgc aagttctttt
    16021 gcagctgctt catcttcagg cgtacatgtt ggtgatttga acaacaatgt atctgaaatg
    16081 atagctgaga gcatgattcc tgcgatttct ttaggaattg ttacttggtt ttctttatat
    16141 agttttaaaa taatcgtact tgtacatcca actggttctg cacgatagta caatggatta
    16201 gctgtttcaa agtttgcaat acgatggtgg tctactacag ctaaaatatt tacttcgctg
    16261 atatctgaaa tactttgttg gaattcatta tgatccacaa gcatgacatc tgcattttct
    16321 gctttagctg actctactac tcgtggtgca gatagctgaa agtggtctaa tgcgtattgt
    16381 gtttcttcat taggtgtacc tagagcaact gcttctgttt ctttacctaa ttcattttgc
    16441 aagtgtgaaa acgcaatggc tgccccgatt gcatctgtat ctggattttg gtgaccaaaa
    16501 actaaaattt ttgacatgtc attcgctcct ttgtgatttc tcctatttaa taatagcaaa
    16561 aaaacggaaa gaagcaatac ttatcttctt tccgatgcga cctatcgcgt taaatatttt
    16621 gtatactctt ctgtatgaag caatcgttta gcattttcga cacgttcttt tgtaggtggt
    16681 tcgatccctt ctaatggata tttcaaacca agtgtttccc acttgtattt tcccatcgta
    16741 tgataaggaa gaatctctac tctgtctaca tttgacaagg tttgaataaa gttgtctagt
    16801 cggatcaaat attcatcata atcacttcgc tcaggaacta gtacatggcg aatccaaacc
    16861 ggtttgttga tttttgacaa atattgagcc atttctaaaa tattttcatt gtcccaatgc
    16921 gttagttttt tatgctcttc attatcgatg tgtttgatgt caaataacag taaatctgta
    16981 tacttcatca attcttgaaa acgtgaaaag aaaggttctt catacgtaaa aggttttccg
    17041 catgtatcca atgtagtatg catcttccgc tcttttgctt ttttaaatag ttcgatcaaa
    17101 aaatcgatct gcaatagagg ttctccacca ctaacagtaa taccaccttt atctccccag
    17161 taaggtctga agcgttctgc tttatctaaa agttcatctg ctgtgtattc tttccctccg
    17221 ccaatattcc aagtatctgg attgtgacaa aattggcaac gcatgcggca tccttgcata
    17281 aacacgacaa agcgcagtcc aggtccgtct actgaaccga aagtttcgat agaatgaaca
    17341 tagccgattg ttttttcttc catttaagtt cacatctttt ctttgatttc attttaagta
    17401 caagttagct gtcgaagcga agctttctga acaaaatttt tcgcaattgc cgcttcgtta
    17461 tgtgtaaaat ttacagggac ggaacataca agtcctgccc ctgtaaatcg tttctttcaa
    17521 ataagcatac tgattattcg attttgatgc gatggctatt caacgtcctt tcgacgctaa
    17581 gtcgaaaaac ttttttcacg aactttttac attctgtcgt gtgaagttct catcaagaca
    17641 tctaattgtt gttctcttgt caagtcacgg aatttaactg cataaccaga tacccggatc
    17701 gtcaagttcg gatatttcat tggattttcc attgcatcac gcaataaatc attactgaat
    17761 acattgacat tcaaatgata agctcctttt gagaagtatc catctaagac attacgcaag
    17821 ttttcgattc ttgtatcttg ttcttttcca agacctgaag gattgattgt ttgggtattt
    17881 gaaattccat ccaatgcaca gccgtaattc attttagctg ttgagttcag gctagctaat
    17941 aacccacttt gttccgcatt gtaacttgga tttgctcctg gcgcaagtgg ttttccagct
    18001 tcacggccat caggagtatt tcctgtattt ttaccgtaaa caacatttga agtaatcgtc
    18061 aataatgaag ttgttggttt tgagttgcgg taagttttat gacgtttgat ttgtcccatg
    18121 aagtattcca agatccatga aactaattca tctgcacggt catcatcatt tccgtattta
    18181 gggaagtcgc catccacttc aaaatcaact gctattcctt tttcattacg gattgctttt
    18241 acttttgcat atttgatcgc tgacaagctg tctgccgcat gagagatccc agcaatacct
    18301 gtagcaaatg tgcgttgcaa gtcagaatcc atcaaagcca gctgagctgc ttcataagaa
    18361 tatttatcat gcatataatg aatgatgttc aacgtattga catatagttc tgctaaccaa
    18421 tccatcatat tcatgaagtt atgtttcact tcttcaaagt ctagtatttc tgatgtgatt
    18481 ggacgatatt ctggtccaac ttgggcgcca gtcacttcat cgactccgcc attgatggca
    18541 tataatagtg ttttagccaa gttagcacga gcgccaaaga attgcatgtc ttttccgaca
    18601 actgttcctg atacacagca agcaatcgca caatcatcag aaccccattg ttcacgaagc
    18661 tgattatcat tttcgaattg aatcgaagaa ctttcgatag cgattctgct agcatatgtt
    18721 ctgaatcctt ctggaaggtg ttcagaatac aaaacagtca agtttggttc tggtgaaggt
    18781 cccatatttg ttaatgtatg aagcagacgg aaatcattct ttgtaactaa tgaacgacca
    18841 tctaatccca tacctgcaat ggataaagta gcccagatag ggaatcctga gaataattgg
    18901 ttatattcag gtgtacgcgc gaatttgacc atacgtaatt tcattactaa gtggtcgatc
    18961 aattcttgtg cttctttttc tgtgattact ccattatcca aatcacgttg gatatagata
    19021 tcaaggaatg ctgagatacg tccgattgac atcgcagcac cattttgtga tttgattgct
    19081 gctaaatatc cgaagtatag ccattggaca gcttcttttg catttgcagc tggtttagaa
    19141 atatcatagc catatttcgc agccatttct ttcatttgcg ctaatgcacg atattgttca
    19201 gtgatttctt cacgtaaacg aatcacttct tcactcattg tatgataacc agtcatatca
    19261 tgatcttttt tcttttgttc catcaaatag tcgattccat acaatgccac acgacgatag
    19321 tcaccgatga tacgtccgcg accgtaagcg tctggaagcc ctgtaatgat tttgttttta
    19381 cgagcagcac gcatttcagc agtataagcg tcaaatacac cttgattatg tgttttgcgc
    19441 cagtcagtga aaatatgtgt caattcatcg tttggtgtat agccattgct ttttagcgca
    19501 ttgttagcca tattgattcc gccgaacggc atgaatgctt gtttcaaagg tttgtctgtc
    19561 tgtagaccga cgattttttc caaatcttta tccaaatagc ctgcatcgtg tgaagtaata
    19621 gttgatacga tttctgtgtc catatcatag acaccgttac gctcgaattg gatatcattt
    19681 aacttttgca attcatccca taatttttct gttgcttctg ttgggccttc caaaaagctg
    19741 tcatcaccgc gatattcagt gtagttattt tgaataaagt ctctagtgtc tacgccttct
    19801 ttccatttgg tacctttaaa tccattccat tgctccattg tatggacctc ctaaatttct
    19861 gttttatgta acagttttta cgtgattagt ataacacagc accctatctt tgcaagggtt
    19921 ttcttgtttt ttatttaaca atttttatgt tttatttaca tatatttaaa tcaacgttga
    19981 cataccaaca tttaagcatt gtgaacactt tcatcttttt ttgcaaacgt ataaggattt
    20041 attaaaaatg aatgaaaaag agacgccacc tgtattaata cagatggcgt ctcttcatta
    20101 aaatactaca acagttacga ctcgatgaaa atctcaggag catcgataac ttcgatgact
    20161 tctccacctt ttttctcatc catcgcaaat gatccattcg atgttcgatc acctataggg
    20221 aaatcagttg ggtcaatcgt taactgctgc cctttttgat tcatcaaggt tatttctttt
    20281 gatcttcctt ccgacatata gataatacgg tgcggatttt ttttcagctc acgcatgacc
    20341 gtcactcccc gcttcgcacg tgaagtttgg ctgatttctt gagcaagcat gcgtttaaca
    20401 gctcctcgct gagtaacgat tacgatcggc gtatcacctt ctgctaacac aagcagcccg
    20461 ttgataatcc tatctccatc tttcaaattc atagacttca ctccagctgc ctttgctcca
    20521 acaacgggaa catctgttaa aggatagcgc aaacccattc cttgatgcgt tactaagaaa
    20581 acatctaagc ctggctgatc ttctttcaga tacacagcaa tcagttcatc ttcttccgcc
    20641 tttagcttca tacagctgag cggacggctc ttataggtgc gccatggttc gaactctgtc
    20701 attcgtgttt gcttgatcaa tccattcttg ctgatgaata caaatgtttt ctctgcttga
    20761 attttttgat atgggaaaac agctaaaatc gattcgtcta cagacagatt ggagattgtt
    20821 tgagaaatgt gctcaccagc atctttccat ttcaaatcgg gtagttcatg gacagggcgg
    20881 tagatcacat ttgctttatt tgtgatcaga agaacatggt ccaatgtact caattcacct
    20941 gagtatagca agtagtcgcc ttctcgcatc ccgatttctt ctggtttgga agcactataa
    21001 gaacgaaggc ttgttcgctt cacatagcct tctcgcgtca cactgacaat tacgtcttct
    21061 tgtgccacta agacttgcgt atcaattttg atttcttcga tttcgtcttc gattgtcgtt
    21121 agacgagcgg aagaataatt ttttttcact tctcgcaatt cttttttcat aacagagaaa
    21181 agctctttgt catttgaaag gattttattc aactctgtga tttgcgcaat caagctttcg
    21241 gattcctttt gaagctctgt gatatccgtg ttcgtcaaac ggtataattg caatgtgaca
    21301 attgcttctg cttgttcttc tgtaaattga aagacgtcga ctaaattctt tttggcgtct
    21361 tttttgtctt tactttcacg aatcgttgcg attacttcat ccagaatcga taaagctttc
    21421 atcaatcctt ctacgatatg ctgtcttttt tgagctttgg ctaaatcgaa ttgactacgt
    21481 tttgttatga cttgctttcg atgctcgata taacttctta aaatgtcttt cagtccaact
    21541 tgatgcggag tcatatggtc gatagcgacc atattgaaat tgtaattgat ctgtagctca
    21601 gtgtttttga acaaataatt caaaattcct tgagcattgg cgtctttttt caattcgacg
    21661 acaatttgca gacctgttct atcactttca tctcgtactt cagctattcc atcgattttc
    21721 ttattcaaac ggatctcatc catttttttc accaaggtcg ctttatttac ttcatatggg
    21781 atttctgtga tcacgatttg ctgtttatta cccttcattg cctctatctt tgttttcgaa
    21841 cgaagaataa cttttcctcg tcctgtttca taagcttttt tgatttcatc ttttccttgc
    21901 aagatccctc ctgttggaaa gtcaggtcca ggaatatatt ccatcaattt ttcaaggctc
    21961 gcttgtggat gatcgatcat atagattgtg ccatcgatca cttcagctaa gttatgagtc
    22021 gggatttctg ttgcatatcc agcagatata cctgtcgatc cattgacgag aaggtttggg
    22081 tatcgagctg gaaggactgt tggttctttt tctgtatcat caaaattcca cacgagatcg
    22141 actgtttctt tctcgatatc tgctagcatc tctccactta acttggataa tctggcttca
    22201 gtataacgca tcgcagcagg tggatcaccg tccatactcc cgttattccc gtgcatttcg
    22261 atcagtactt cccgcaactt ccagtcttgg ctcatgcgga ccatcgcttc ataaatactg
    22321 atgtcaccat gaggatgata attccccatg atatttccga cagattttgc tgatttacgg
    22381 aatcctttgt catacgtatt gccatctttg ttcatggcaa ataatatccg gcgttgcact
    22441 ggcttcaatc catcacggat atcaggcagt gcacgttctt ggataatata tttggaataa
    22501 cgtccgaagc ggtcacccat cacttcttct aaagttaatt cttgtacttc ttgtcgattt
    22561 tccatcaggt gacctcctat tctacttcaa ataactgatt gttttcattt ttatctgctc
    22621 gttcttcatc taataaatca ttagaaactg aaggagagat ttcagtatcc tcttttttat
    22681 ctaagatact gccatcttct tcgagcgtaa attgcacgtg attttcgatc cattttcttc
    22741 gaggttctac tttgtcaccc atcagtgtgg ttactcgacg ttcagcttgc gcagcatcat
    22801 cgatacggac gcggatcaac gtacgggaag tcggatccat cgttgtttcc cacaattgtt
    22861 ccgcattcat ctctccaagt cctttatatc gctgcagcat atatcctttg ccgacttttt
    22921 taatcattgc tgccaattcg tcatctgtcc acgcatattc aatgacttgc ttctttcctt
    22981 gtcctttgga tactttatat aaaggcggta acgcgatata tactttccca gcttcaatca
    23041 gcggtttcat ataacgataa aagaaagtaa gcagtaatac ttgaatatgg gcgccgtctg
    23101 tatccgcatc ggtcatgatg attactttgt cgtaattaca gtcttcgata gaaaactcag
    23161 gaccgactcc tgcaccaatc gtatagatca tcgtattgat ttcttcattt ttcaagatat
    23221 cttgcatttt agctttttcg gtattgatga cttttcctcg taaaggtaga attgcttgga
    23281 actttctgtc tcttccttgt ttagcagatc cgccagcaga atccccttcg actaaataca
    23341 attcattttt cttagggttt cgggattgtg cgggtgtcaa ttttcctgaa agaagagatt
    23401 cgcctttttt acgcttcttc ccatttcggc tttcttctct ggctttacgt gctgcttctc
    23461 tcgcttctct ggctttgatt gctttacgga ccaacatctg gctcatttcg ctattttcct
    23521 gcaaataaaa gcccatttgt tcactgatga cgttatctac tacggtacga gcaactggcg
    23581 taccaagttt ttcttttgtt tgcccttcaa attgcagaag attttcaggt acacggatag
    23641 aaagtacagc agctaaccct tctctgaaat cactaccttc cagattcttg tcccgttctt
    23701 tcagcaaacc aacttttctc gcatattcat tataagattt cgtcatcgcc gctttcatac
    23761 cagcttcatg ggttccccca tcttttgtcc gaacgttatt gacaaaagat aaaacatttt
    23821 ctgaataacc gtcattgtat tgataagcga cctctacttc gataccttct ttttctcctg
    23881 aaaaataaac aactggtgtc aatgtatctt tttcttcatt cagataatca acaaattctt
    23941 tgattccttc ttcgtaatga aaaatttcct tgactggttc ttctccacga agatcagaaa
    24001 gagagatttt aacgcctttt agaagaaagg cagattctct taaccgttcg gctaaaattt
    24061 catatgaaaa tttcgttgta ctgaatattg tatcatctgg taaaaaatgg acagtcgtcc
    24121 cgtttgcttt ttttgttttt cctatctttt tcaaagtacc gtcaggttta ccgccgtttt
    24181 taaatttctg ctgatactct acgccatctc taacgatcgt tacaattagc catttcgata
    24241 aggcatttac cacactggct cctacaccgt gcagtccacc agaagttttg taccctcctt
    24301 gaccaaactt tccgccagca tgcagcacag taaaaatcac ttcaactgtt ggtattccgg
    24361 aagcatgcat tcctacaggc atcccacgtc cgctatctgc aacagttata ctattgtctt
    24421 catggatggt cacatcaatc tcactgccat aacctgataa tgcttcgtca actgcattat
    24481 cgacgatttc atagactaaa tgatgcaagc ctcggctgtc agtggatcca atgtacatcc
    24541 ctggtctttt tctaactgcc tctaatcctt ccaatacttg gatggacgag tcattgtatt
    24601 cgttgtttac tttttttgcc aaggcaaacg ctccttcaat aatactttgc ggcaaaaccg
    24661 caccacgatt atcatactag aaaaggtcgt gaaaaaaaag agggtagacg agaaatcaat
    24721 tttaattatc tcctaaccgc tttttcgaca ttcccctcta tcgatcatct cttctttgtg
    24781 gtcattttta tttttggttt tctttcaact taccaagttc aatcttaata caacgatcca
    24841 ttacgatatc tgtacgtcct gcttttctta gcatctcagc tgcttctttg ttttcaatcc
    24901 ctaattgtgc ccagaacact ttagcatcag ttttcaaaaa atcacctgct atttcaggta
    24961 aaaattcact ccgtctaaat acatctacga tatcgattgg atcatcgatt tctgtcaaat
    25021 tcgcatagac tttctcgcct agaatttctt caccagccaa taatggattt actggtatga
    25081 ttttatatcc attgtcttgc aatacctgtg caaccatgta acttgttcgt tctggtttat
    25141 tgctcaagcc tacgacggca atattttttg cttcttccaa atagttgaaa atcgtttctt
    25201 gtgttggatt ttttacagac atttctgcca ccccttgctt ttctttttat tccatctatt
    25261 atacttgtat ctttcatttt ttcaattgtt cctgttacct attcatgcta aaataaagga
    25321 tagttttcaa cttgaaagag ggtaaaaaat gaaattgatc ttattactta ttttagcata
    25381 tcttttaggt tctattcctt ccggtgtctg gattggcaaa ttgtttttca aaaaagatat
    25441 ccgacaacac gggagcggta atactggaac gaccaataca tttcgtgtat taggcaaaac
    25501 agccgggatt gttgtcctgc ttatggatat tttaaaaggt acattggcta cttgtttgcc
    25561 gatgatcttc cacctttctg ggattaaccc tctttggttt ggggtatgcg ccattcttgg
    25621 ccatacattt ccgatttttg ctgggttcaa aggaggaaaa gctgttgcga caagtgcagg
    25681 aatgctttta ggatttaatc ctgtattttt tgtttattct tgtatcatct ttatcgttag
    25741 tttattttgt acaagtatgg tgagtttgag cagtatgatc agtgcagtat tgatcacttt
    25801 atctacgatc gtccttcctt atgtagctcc tgtgattttg gctaagccca attggttact
    25861 gacgatcatt gcctttttag tttcttcctt tatcttttat cggcataaag acaatatcaa
    25921 gcgtatcaga aaccatactg aaagtcgaat cccgtttggt ttgaatagca gcaagaaaaa
    25981 atcctgatac tttttcattc caaagagata aaacgggagt gtgacaaatc gatgtcacac
    26041 tcccttatta cttacaggat agctttttta cacaccttgg taaacggtgt tccctgctaa
    26101 cttctcggtc acaacctctt acttcactgt gatggaatat tttgtcttga agatttcatg
    26161 ggcacctagt tgattgatcc ctactttttc catcaagttt ccactactgt tcaccgtgtc
    26221 agcgattccg caccacggtt cgatacaaac gaaaggcgct tcttttggat aaggagacca
    26281 gatccctaca taaggcatat ctttatagct taaagttacg ctatgggaag atttttcact
    26341 tcgaatggcg tacgcattta gaccccttgt ttcaaaaacc aaggcatctt gctcaaaaag
    26401 ttcacgattc aaagccagat tcgtatttgt ctgacctaac gtcttttgat ccatatcgat
    26461 gaatgggcct ttcaatggta ggcgtaatct tgatttcaat ggagaaaatg ccagatagaa
    26521 atcttcaaat gacaatcctt cttcaagagg tacattgaaa gcaggatgtc caccgataga
    26581 aaagaacatt tctttttcac cggtatttgc cacttgataa tgaaccgtga tgccgtctcc
    26641 tcctaattca tagctgatca ctaattcaaa atcaaatgga tacttttcta atgtctccgg
    26701 cgtactcctt aagatgaaac tagccatttc ttgtccatgt tcaatgactt caaaatccat
    26761 atcccgagca aatccgtgtt gccccatttg atatgttttc ccttgataaa catactgatc
    26821 atcttttaaa cgtccaacaa aagggaataa aaccggtgca tgtctgcccc aatacgctgg
    26881 atcaccttgc cagatatact ctaaaccatg ttcttttgat ttcagactga tcaattccgc
    26941 accgtgttca gcgatttcgg cgatcagctg gtcattttga ataataacag tcattgccat
    27001 tcctccaact tcgacttact ttttaaaatg ggaaggctgg aacaaacatt tctatctgcc
    27061 ccagtcccct tacttctaat aactgtgttt tgctaactcg tctttaaaac gggaattcaa
    27121 tacttttaaa taagttccct tcatgcctaa cgaacgagat tcaatgattc cagctgattc
    27181 caattttcgt aaagcattga cgatcacaga acgagtgatg ccgatactat cggcaatatt
    27241 agaagcagtc agacgacctt catctccatc caatgcttca aagatagctt gtacagcctt
    27301 caattcgcta taagataatg tgttgattgc catttggaca gccgttgcac tccgtacatc
    27361 tgcttcgatg cttcgagact tctgataaag gatctgcata ccgacaaccg tagctccata
    27421 ttctgctaag actagatcgt catctgcaaa cgattgttcc atacgagata agataatcgt
    27481 acccaaacgt tcgccagctc caaagatcgg aacgacagtc gtcagaccaa atggatattt
    27541 ctctcttgac tctaccggaa atgcagtaat atcactagta attgggatat tagcttcagt
    27601 tttcatcaac tgtgccactg tatcagtata agattcagga aattgtttct caacaaacat
    27661 tgttttgatt cgttcattgt tcacatcgtg acgttcatta taacctagta atactccgtc
    27721 acttcctatg atataggtgt tgctgtcaag aataccacct agtgtgacag ccattttatc
    27781 gtaaggcaaa tctgcttgca tgtcaaaagt attcttttgc tgcaaaagtg tgtttatctt
    27841 gcgggttttc gttagcaagt ctgtcattgt cgtcctccga ttttttataa aatatagcga
    27901 cttaaatctt catttgccgc aattgagcca agtttgtcat ccacatatgc ttctgtgatc
    27961 gttatttctc ccatttgcat atctggtgct tcaaataata gatcttctag tagtttttcc
    28021 aaaatggtat gcagtctgcg tgcgccgata ttatctgtgt cacggttgac atcgaaagcg
    28081 atttgagcga tccgttcaat tgcttctttt gtaaacgtaa ctgacacatt ttctgtaccg
    28141 atcaacgcga cgtattgttt tactaaagca ttatttggtt cggtcagaat cttcacaaaa
    28201 tcttcagctg tcaaatcatc taattctacc cgtatcggga agcgtccttg aagttctgga
    28261 attagatcac ttggtttaga caaatggaaa gctcctgaag caatgaatag gacgtgatct
    28321 gtttggatga tcccgtattt tgtgtttact tgtgaacctt cgacaattgg caatatgtct
    28381 ctttggacgc cttctctgga tacttctcca gattgctgtg atttagaagt gatcttatcg
    28441 atttcatcga taaaaataat tccactttct tccgctaatt tgattgcctc actatggata
    28501 tcagcatcct tgactaattt tgctgattct tccttgatca ataactctaa agcttctttg
    28561 actgttaccg tccgttccac ttttttcttc ggtgtcaaag cacctagagt ttcatttagg
    28621 tcgatcccca tttgctccaa tccattgttc atcattggtg caggtttctt ttggtcttca
    28681 acttcaatgg ttacttcacg actatccagt aaaccctttt gtagttgttc caagactgta
    28741 cggcgattga ttcgaatatc ttctgtcact tcttcttgcg gttcagcggt attttgctga
    28801 ctagcattaa aaagattcat catttgttca aattgattag tggattgttt ctgttctttt
    28861 ttgataccag gtaccaagat ttttaccaaa cgtttttctg catttttttt agcacttgtg
    28921 tatacacggc tgtactgctc tttttctacg atttggatcg cattttctac taaatcccga
    28981 accattgatt ctacatctct tccgacataa ccgacttcag taaattttgt tgcttcgact
    29041 ttgacaaatg gtgcattgac gatttttgcc aaacgtcgtg cgatttctgt tttaccgacc
    29101 cctgtaggtc cgatcatcag catatttttt ggtgtaatat cttgttgcat tttttcatct
    29161 aactgcaaac ggcgatagcg gttacgcaaa gcaacggcaa cagatttttt tgctgcgtgc
    29221 tgtcctacga tatattcatc taattcactt acgatttgtc ttggtgtttg ttttaattca
    29281 tacatatcta ctcacctctt acagttcttc tacgataata ttatgattag taaagataca
    29341 gatatctgcg gcaatattta acgcattttc tgcaatttct ttcgcactca tgtcgttttg
    29401 agcatagttt ttcattgctc gggctgcagc caacgcaaaa ttccctcctg atccgatagc
    29461 taagatacca tcatctggtg tgataacttc tcctgtacct gaaacaagaa gcatttcttt
    29521 atcattcatt acgatcagca tcgcttcaag tttttgcata gattgctgtg tccgccattc
    29581 ttgagctaat tcgacagctg cgcgttgtag attcccgtta tattcgttta gtttgccttc
    29641 aaatttttct tctaaagtaa acgcatctgc aacacttccg gcgaaaccta caacaacttc
    29701 tccattgtag atacgtcgta ctttctttgc agtgcctttc atgacaacag attcacccat
    29761 tgttacttgg ccatctccag ccatagcaaa tttcccgtct ttttcgacgg cacagatagt
    29821 ggttgaatga aatggtgatt cgaccatgtt tgtttcctcc taaatttgtt cttttcctta
    29881 tttttacgct cttggatgga aagaacgata gtttttttgt aaactttctt ttgttacatg
    29941 ggcatagatc tgggtagtcg ataaattggc atgtcctagc aattcttgga ctgtccgcaa
    30001 atctgcccca ttatttaata aatgagtagc aaatgtatgg cgcagcatgt gaggatggat
    30061 atcactatcc aaactgcttt ttttgatcat ttgattcaag acatattcaa ttccagtagg
    30121 cgtgattggt tttccatgat gattcacaaa aacgatatca tgctcttcgt gatattttgt
    30181 catcaatacc tggcggcctt ccctgaaata ttcttgcaga gcatcttgag caaaagaacc
    30241 tagaggagca tatctctctt tgttcccttt accatggatc aacatgacct ctgaatcaaa
    30301 atcaatgtct gataccgaca aactgctaca ctcacttaaa cggatgcctg acccgtaaag
    30361 gacttccagt aaagcgcggt tcctcaaatc caaaggacta tctcctgcta cgctgtcaaa
    30421 aagtgcttgc atttcatttt cataaaaaaa acgtggcagt ctcaaatttt tctttttgag
    30481 atgaacgtaa gaaaatggat tttcttttat cacttcttct ttgagaagat actgataaaa
    30541 cgaacgtagg ctggctatct tgcgactgat agaatttctg ctatagctag gacgatgatc
    30601 agctaatgca ctcaaataga cacgaaccgt aaaatattcc gccttcagca gatcagcgtc
    30661 tccagattcc tccaaaaaat gtttgaaatg gagaatgtct tcttcatatg cttctttcgt
    30721 tttttctgaa tatcctcttt cgacaatcaa ataacgcaaa aatttttgtt cccacgtggc
    30781 ttctgtcatt ttctcacctt ccttttgtcc cagagaataa tttagcacag cgtttataaa
    30841 aagacaaata aattgtcaga atttaaccaa tatttctaag tttgttcaca attggcgcaa
    30901 ttcctcacat tttgtttgag aaattcttaa gaaaaacgca agaaaaagag cgagactatc
    30961 cgtctcaccc tcttcatgga gtttccttac tcatttggga ttgcagtttt ttctaattct
    31021 gtttttattg cctcattagc ttttaaagct cgttgtgcaa ttgcttcata tcttgctttt
    31081 ttgtctcgaa tgcgctctgg cagttcagga aacagaccat agttggcatt catcggttgg
    31141 aaatgttttc cttccgcatg agtaatgtag taagccatac ttccaatagc tgtttcttgt
    31201 ggaaactcga ctggttccat ttcttttgct aagcgtgcgg cgttgattcc agcaagtagt
    31261 ccgctcgctg cactttcaac atagccttct acaccagtca tctgacctgc aaagaacaga
    31321 tcttcgcgtt tcgttgattg gtaagtcggt ttcagcaatt ctggcgaatt cataaagctg
    31381 tttcggtgca tcacaccata gcgaacgaat tcagcatttt ctaacccagg aatcatccgg
    31441 aaaacacgtt tttgctcgcc ccatttcaag tgagtctgaa agcccacgat gttgtataaa
    31501 gaagctgcag cattgtcttg acgcagctgg ataacagcat aaggacgttt gccagtttta
    31561 ggatcttcca atccaactgg tttcataggt ccgaaaagca tcgttttgaa tcctcgctgt
    31621 gccattactt caattggcat acatccttca aaatattttt ctttctcaaa agatttaagt
    31681 tctgccactt cggcattgat tagtgcctca tggaaagctt tgaattcctc ttctgtcatc
    31741 ggacagttca agtaagctgc ttctcctttg tcatagcggg acttcaaata tactttatcc
    31801 atatcgatcg tagctttgtc tacgattggt gcagctgcat catagaaata aaaaccatct
    31861 gaaccattga atgctttgat gctttctgct aatggatgag aagtcaaagg tcctgttgct
    31921 actacggtga ttccttccgg tatttcagta atttcttcgt ttatcactgt tactaatgga
    31981 tgctctttga cttttcgggt aattgtttca gaaaaagaat cgcggtcaac agctaaggca
    32041 ccaccagctg gcactgctgt ttcatctgcc gaagtgatta ctaccgaatt caatcggcgc
    32101 atttcttctt tcaataaccc tactgcatta gccaaactgt ttcctcttaa tgaattggaa
    32161 catactagtt cagcaaaatt acttgtgtga tgcgcctcag tcgatttgac tggacgcatt
    32221 tcatataaac ggacagggac tcctgcttgt gcgatctgcc aagcagcttc acttccggca
    32281 agccctgctc caacaacatt taccattttc aatgaaagaa cctcttttct attatttttg
    32341 gatgtcttct tcgtaatctc catttacaca gactacttgt ttaccgcctt tgactttctt
    32401 ctcgaccaga tatccgtcac attttggaca atttctgccc actggtttat cccaagaagt
    32461 gaattcacat tctgggaaac gggaacaacc atagaatagt ctgtttttct tagattttcg
    32521 ttcaatgact tgtccttcat gacatactgg gcaagtcaca ccgatttctt ttacgattgg
    32581 tttcgtattc cgacaatcag gaaaattact gcaagcataa aacttcccat atttccctaa
    32641 ttttatcacc atcggatgtc cacagacatc acaatcgaat cctgctggtt catctttgat
    32701 ttgaattttt tcgatttttt cttccgcatt cgtcagttct ttttcaaacg gtttatagaa
    32761 acgatcaacc actttgaccc acgcttccgt gccttcttca attttatcca aatctccttc
    32821 catttctgct gtgaaatgga tatcgacgat ttgagggaag aattcacaga ttaatgaatt
    32881 gacaatttct cctagttcag tcggctcaaa tcttttttgt gtcaatttta catagtagcg
    32941 tctttggatc gtatcaagtg ttggtgcata agtggaaggt cgccctaccc cgttttcttc
    33001 tagtgcacga atcaacgtag cctcactaaa acgtgctggt ggttgagtaa aatgttgttt
    33061 tggttcaata tcaacagatt ggactacatc gccttcttca agttctggaa gaatattttc
    33121 tttctcttct ttgccgtcat ctctaccttc cacatacact tgcatgaatc ctttgaattt
    33181 gatttttgaa ccgttcgcaa tgaagatgac accattttgt tctaatgtca ctttcattgt
    33241 atctaaaata gctggtgtca tctgactagc cacaaaacgt gaccagatca atgtataaag
    33301 tttcaattga tctttatcta aatattgttt gagttcatct ggtgtacgaa gcacactcgt
    33361 tggacgcaca gcttcatggg catcttgtgc gccttgagca tttttgatct ttcggtgatc
    33421 atgtctagaa aattcttttc catacgtatc ttcaataaat gctgcggcct ctgcttttgc
    33481 tgtatcggca atccgggtag aatcagtacg catgtatgta atcaagccga ctgttccttg
    33541 tttacccaaa gtgattcctt catataattg ctgtgctacc atcatggttt ttcttgttcg
    33601 aaagtttaac ttacgggcag cttcttgttg caggctactt gtagtgaaag gattcgcagg
    33661 gtttcttttg cgctctttct tttctacttt tttaacttcg aagtctgttc catcaattct
    33721 agcagtgact tcttttacac tttgtgcatc tggtaatttc tttttcttac cgtcgattcc
    33781 ccagaaattt gctttgaatt tcttgcgagc ttttttaaaa ttaccgtcga tgctccaata
    33841 ttcttctgga acaaattctc ggatctcttt ttcacggtca ataataattt ttaaagcgac
    33901 agattggaca cgtcctgcac ttaacccttt cttgacctta cgccagagaa taggactgat
    33961 cgaataacca acgattctgt ctaaggtacg acgagcttgc tgtgcatcta ctaaatctac
    34021 atcgatcgtt cttggttcct taaaagctgc tttgactgcg tcttttgtga tttcattgaa
    34081 gaccacccga tttttttctt tcagatccaa cccaagtagg tacgctaaat gccaagcaat
    34141 cgcttctcct tctctatccg ggtcacttgc caagtaaact ttttgagctt tttttgctgc
    34201 ggctttcagg cttttgatga catcgccttt tccgcgtata gaaatataat ggggttcata
    34261 attgttttca aaatcgatac ccattttact tttcggtaaa tctcttatat ggccaacact
    34321 ggccattact ttatagtttc gtccaagata tttctcaatt gtcttggctt ttgcaggtga
    34381 ttctacaatc actaaatatt tatacgccat tactgaatgg actcctttcc attgtcgtgt
    34441 tcctactatt aaaagaggat tcatacaaat cgtagctaat catatccatt cagaaaacgt
    34501 ttgtcaagaa aagcaaatga aaagtatggt gttattcttt aaattctgcc aagatatctt
    34561 cacccgaaac cgtgcaaact gcaccgtctt ggatcaaatg atgacatccg gtagaatgtt
    34621 ctgttaaaat atttcctgga acagaaaaaa ctgttcttcc aagatccaat gcttgctgtg
    34681 ctgttatcag agagccgctt tttttcttgg cttcaaagac acatgttcct ttagacaaag
    34741 ctgcgataat ccgatttctc attggaaaac aatgcttttt cgggccggat tgaggaggat
    34801 attcactcaa aagaagatga tcatttgcaa tcacctcttg caagtcacga ttttcagcag
    34861 gatagacttg ctctatccct gttccaatga ctgcgattgt ccgtccacca tgagagattg
    34921 ctgcttggtg accgtatgta tctatccctt tcgcgcagcc actgacgatc acatatccag
    34981 cttctatcac tggttttagt aaataataaa ctgttttttt ggctaaagca gaagtgtgtc
    35041 ttccaccaac aacgcttaac atgttacaag aaagtaaaga tagatcacca ttataaaaca
    35101 gtacaattgg cggataggtc aagtgaagca gctgaggggg atatttggga tcaaagcaag
    35161 tgataaacga ctgtttcgat tggattttgc cccagttagc gtgaatttcc tgccaacttt
    35221 tgcgaaacag ctcccggtgt ttggttattt tacctatctt aatgatgtct tcaaatccga
    35281 tttctttata aggatactgc atcatccagt tagcaagtgc ccattttcct tgactactga
    35341 ttccttttgt ataagcaagt ttcaataaat tttcttctat ttgatacatc ataaaaccgc
    35401 ctttcgtctc tctttctaat aagatacgta aaagcggctg ttttttattg taaatatttt
    35461 tttacaggtg caaaactttt acgatggatt ggcgtaattc cttgtcgttc aagtccttct
    35521 aagtgttcct ttgttccata acctgcatta tgcgagaaac catatccagg ataaagcacg
    35581 tcatattctt tcatcaattg gtctcgatat actttagcaa tgatacttgc agcagcaatc
    35641 gacacagatc gtacatcccc tttgattatt ttttcctgct gaataggcaa atccaaggtc
    35701 atcgcatcga tcaacaaata gcttggtaca atggacaatt tttctactgc ttggatcatc
    35761 gctaatttac ttgcttcata aatattcaca tcatcgattg tctgctgatc aacgatcccg
    35821 atcccgattg ccagggcttt ttcttgaact tgttgataaa gaagttctcg ctttttctct
    35881 gataattgtt tggagtcatt cagccctaat atgatctcat ttttcggcaa gataactgca
    35941 gctgccacga caggtccagc taatggtcct cttcctactt catcaatgcc ggcaatccat
    36001 tggtgacctt gtgcgtaccc ttgcttttca aagcaaaaca tttcttcata tttttcctta
    36061 attaatgctt cctttttcaa tcgtcgttca aaagaagcca gtgcttgctg cacgcctttg
    36121 cgtggatcat ttcttaatga ttctagtgta ggatcatcta ttctctgtat gcttttcaat
    36181 cgttctttga tactttggat ggattcagtc atcacttttt tgctccattt cttcgcaacg
    36241 atccagtgtg tatcgtccaa gttttccttg acgaatatca tgcacgacta attcgctgcc
    36301 acgatcatag tcttctttat acccacgttt tgctgtaata tccatcagta attccggtgc
    36361 caatttttct tcttgctcga ttgataaacc ataacgatcg ctgattcttc ctgaataata
    36421 tcgggcaaaa aagtctaatc cgtaaatcgc taaatcatct aaatggacaa gctggtcttt
    36481 gatcgcgcct gtcagcgcta gttttttgcc gatttcttca tcttcaaatt tgggccataa
    36541 aatccctggt gtgtctaaca attccaactc attacctaaa cgaagccact gctgaccttt
    36601 tgttacgcca ggcttatttc ctgtctgtgc aattttcttg cctactagtc gattcatcaa
    36661 cgtagatttc ccaacatttg gaatacctaa gaccattgcc cgaatagcgc gaggcttgat
    36721 cccccgagca gcatctcttg cccgtttttc tttcaatgct tctttagccg caggtactag
    36781 acctttaatt cctttacttt cttgtgccgt gatagctaat gcaagatggc cttgtttacg
    36841 aaaatattct tgccacagct ttgtctgttg tggatctgcc agatccgctt tattcaataa
    36901 aacaaggcga ggtttttgct gaatgatctg atctagcatt ggattacgag aagataaagg
    36961 taatcgtgcg tctaccaatt caaatacgat gtccacatat ttcatttttt cagaaacttc
    37021 tcgtcgggct tttgccatat gtcccggaaa ccattggatg gtcattgttc tctccattcc
    37081 tttcataccg gaatattccc cggctttact tgtttttttc tttttcaata taaattcact
    37141 tttttatt
//
LOCUS       DASHFF010000023        36567 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.023, whole genome shotgun sequence.
ACCESSION   DASHFF010000023 DASHFF010000000
VERSION     DASHFF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.023"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="U2I75_001287"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="U2I75_001287"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN1696434.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="U2I75_001288"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="U2I75_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN1696435.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="U2I75_001289"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="U2I75_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN1696436.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="U2I75_001290"
     CDS             complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="U2I75_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN1696437.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="U2I75_001291"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="U2I75_001291"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN1696438.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="U2I75_001292"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="U2I75_001292"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN1696439.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="U2I75_001293"
     CDS             complement(8052..8522)
                     /locus_tag="U2I75_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN1696440.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="U2I75_001294"
     CDS             complement(8568..8993)
                     /locus_tag="U2I75_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN1696441.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="U2I75_001295"
     CDS             complement(9060..9329)
                     /locus_tag="U2I75_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN1696442.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="U2I75_001296"
     CDS             complement(9340..10935)
                     /locus_tag="U2I75_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1696443.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="U2I75_001297"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="U2I75_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN1696444.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="U2I75_001298"
     CDS             complement(14488..15048)
                     /locus_tag="U2I75_001298"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN1696445.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="U2I75_001299"
     CDS             15401..16375
                     /locus_tag="U2I75_001299"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1696446.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="U2I75_001300"
     CDS             16545..17945
                     /locus_tag="U2I75_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN1696447.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="U2I75_001301"
     CDS             complement(18023..18745)
                     /locus_tag="U2I75_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1696448.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="U2I75_001302"
     CDS             complement(18824..19330)
                     /locus_tag="U2I75_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1696449.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="U2I75_001303"
     CDS             19453..21252
                     /locus_tag="U2I75_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN1696450.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="U2I75_001304"
     CDS             complement(21320..22894)
                     /locus_tag="U2I75_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1696451.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="U2I75_001305"
     CDS             complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="U2I75_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN1696452.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="U2I75_001306"
     CDS             complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="U2I75_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN1696453.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24671..25309)
                     /locus_tag="U2I75_001307"
     CDS             complement(24671..25309)
                     /locus_tag="U2I75_001307"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN1696454.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25310..25960)
                     /locus_tag="U2I75_001308"
     CDS             complement(25310..25960)
                     /locus_tag="U2I75_001308"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN1696455.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="U2I75_001309"
     CDS             complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="U2I75_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN1696456.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="U2I75_001310"
     CDS             complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="U2I75_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN1696457.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29103..29843)
                     /locus_tag="U2I75_001311"
     CDS             complement(29103..29843)
                     /locus_tag="U2I75_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN1696458.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="U2I75_001312"
     CDS             complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="U2I75_001312"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN1696459.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31214..32152)
                     /locus_tag="U2I75_001313"
     CDS             complement(31214..32152)
                     /locus_tag="U2I75_001313"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN1696460.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32145..32636)
                     /gene="def"
                     /locus_tag="U2I75_001314"
     CDS             complement(32145..32636)
                     /gene="def"
                     /locus_tag="U2I75_001314"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1696461.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32651..35059)
                     /gene="priA"
                     /locus_tag="U2I75_001315"
     CDS             complement(32651..35059)
                     /gene="priA"
                     /locus_tag="U2I75_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN1696462.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35312..35617)
                     /locus_tag="U2I75_001316"
     CDS             complement(35312..35617)
                     /locus_tag="U2I75_001316"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1696463.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="U2I75_001317"
     CDS             complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="U2I75_001317"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN1696464.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9885 a         7988 c         6706 g        11988 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggcttaatt
    22921 taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt accatagcag
    22981 tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt taaaaatgac
    23041 aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag catttcttaa
    23101 gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt attcatcact
    23161 gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca acagcagtgt
    23221 aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt taaaaatcaa
    23281 ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac tgaaaaattc
    23341 agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata caattaaagg
    23401 gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga tagccccgag
    23461 aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac ataataatgc
    23521 caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc ctagcattct
    23581 tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat agtacttcat
    23641 tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg tcacaactga
    23701 cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc cataactatc
    23761 tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc ctccaagacc
    23821 ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt gggtcaccac
    23881 taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc cattagccga
    23941 agatacccgt actggaaaat gattggagct ttcattagag gatgacagta attcgatttt
    24001 agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg acatgactgc
    24061 catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt cctgccaact
    24121 gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca gcgtctgatt
    24181 caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt tttgctgtga
    24241 aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat aagttgaaaa
    24301 aagattatgg acagaatcta ctccatttcg tacccaccgt actggggcag agattgtttt
    24361 atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct ttccttcatt
    24421 cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa taaccaaggt
    24481 aataataata tttttattag gattgaactt cttcacaaag acacctcgaa ttaattagac
    24541 tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc tcctcttaag
    24601 ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa tttttttatt
    24661 tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa aaagaaaatt
    24721 ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct acttctttat
    24781 tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc aagcagcaat
    24841 aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc aaataataag
    24901 acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg aagcgaggtt
    24961 ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta gctccaatca
    25021 aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac tgtgtatctg
    25081 tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat acttccttct
    25141 gctcttcgtt cgacaatgaa tcaaaatcgc ccactgccag atctactggg tagcctgctt
    25201 gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg gacagcagag
    25261 actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt tagtctaatg
    25321 cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata tatgatcctg
    25381 caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga acgattccgc
    25441 catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg agttccgtaa
    25501 ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg acagtcatga
    25561 ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt gttcctggat
    25621 tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc cgatgaatat
    25681 gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca aatgctggaa
    25741 tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta gtaacaggac
    25801 ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaattgg ccatccatta
    25861 catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct ctttgtaaat
    25921 tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc tcctattttc
    25981 gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt aagaattgca
    26041 aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt tttacttcgc
    26101 agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa gcttctacaa
    26161 attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca atcgagctaa
    26221 atcctggtgt atctgctact aacccgccat agataggaat cagttccaca tgacgtgttg
    26281 tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc agttccggtg
    26341 atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag accgatagcc
    26401 gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt acaatcaccg
    26461 gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg tcgtctgaca
    26521 agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag gtaactagaa
    26581 agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca atgattccaa
    26641 gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag atttccagaa
    26701 gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca agcggcgtga
    26761 ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg tttgcgtata
    26821 catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc acctccgttt
    26881 ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac ttctcaaagt
    26941 atatggcttg aaaattgatc aaaagagata gaaacacaaa aaagagaatg caacaaatca
    27001 atgtcgcatt cccttctgat atattttatt cagcacttat tttgaacttt ctgaggtgct
    27061 agctgatgaa cttgtggagc tggaagattc ttcttttgaa gagttgcttg tgctactgga
    27121 gctacttgag ctgctagaac tgtctgaaga gtctttttca gaactagaag acgctggatc
    27181 tggtccttta gagtaaatta cgttcacaac cattcctttg gtaattgtcg ttcctgcccc
    27241 tggtactgta cgaatcactt tatctttatc caccgtgttt gaatattcgg aagtctcatg
    27301 cccgatatat tctgcaccga cactagctaa ataactttgt gcttcgcttt ttgtatagcc
    27361 ggagatatca ttcaatttag gctctgttcc ttggcttacg acaaaattga tgtaagtttc
    27421 ttctgctaca acttcacccg aggctggatc ctgactgatg atcgaacctg atggtacagt
    27481 gtcgctatag tcataagtag cactaatatt attgatattg aatccttgtg taacaagcca
    27541 attacgaaca gtttcgtagt tttctcctac atgactactc aaagtcactg tttctggtcc
    27601 tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg acttctgtat cttctgatgg
    27661 agattgcgaa atgatcattc cgctttctac ctctgaactg aattcctctt ctttttgaat
    27721 caagtcagca gaaaacccta attctttcaa acttgcttcg gcatcttcat aacttttacc
    27781 actgtaatca tctaatttga tttttttcgt gccgctacta atatacagag tgacttcgcg
    27841 tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc actacattac cttcttcgat
    27901 tttgtcatct gcaatttctt ctgtttttgt tgctacttct aatttcgctt ttgcgagtgt
    27961 ttctctagca gaagcttcag aaagatcaga aacatcagga acaacaactt ctcctcgccc
    28021 gccagaagca aagaatacag ctgttccgat tcctaaaaca gcaagaatag ctaataggat
    28081 cagccaccat tttttctgtc ctttcttttt aggatcaggc agaacttctt cctcctctgg
    28141 caaggtttcg ttttctggct tgtcttttgg cagaggcata gatttgaatg aatcaggcat
    28201 tggtgcatct tccgtaaggg gtgtgataac ttttgtttca ttatccatca catgaggctg
    28261 ccatttttct tcgtttgcac gagctggtga taatactgta gaaagatcat cagacatttc
    28321 ctcggctgac ttataacggt ctgcaggttc tttagccgtt gcccgtaaaa tcacgttttc
    28381 cagtgcttgt ggcacatttg gatcaagtgc ttttatagat ggaagatcat cttgaaaatg
    28441 ttttaaagca attgttacag cagattcacc atcaaacggt acgctacccg tcaacatctc
    28501 ataaaggatg atccctaaag cataaatatc tgactgcttc gttgccatac ttcctctggc
    28561 ctgctcaggg gacagataat gaacagatcc cagcatcgta ttcgtctgcg tgatcgatgt
    28621 ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt acaacacctt cattatcgat
    28681 cagtacgttt tggggtttca aatctcggtg gatgatttga tggttatgag ctaaagaaat
    28741 agcagaaagg atctgctgca taatatttac tgctgtttca taaggaacag gataatgtgt
    28801 ttggatatat cgtttcaagt ccattccttt gacgtattcc atcaccagat actgcatgtt
    28861 gtcttcttca ccaacatcgt aaacactaac aatgtttgga tgaacgagtt ctgttgctgc
    28921 taaagcttct ctctgaaatc gtcgaatcgc agtctggtca ttttgaaagt caaatcgtaa
    28981 gactttaact gctacatcac gatccaagat taaatcgtgc gccaaaaata catttgccat
    29041 accaccgcta ccgatatttc cagtgatttg ataacggcca tttaattttt tgcccaactc
    29101 gatcatacac tgtcctcctt atattcgata agaagaacgg tgatattatc cgctccgcct
    29161 gcttcattgg cacgatcaat cagctcagtt actttatgag acaatgggcc ttcttgattg
    29221 atgatcgttc ctatttcttc ttcagacagc atgttcgtca agccatctga acataacagc
    29281 agtcgatctt ttagttgcca aagatgagta gaaacatcta cttcgactgt tccaggcatg
    29341 ccgacagaac gtgttaaaat atttttcctt ggatggttca ctgccatttc ttcgctgatt
    29401 tctcctgatt tgactagttc attgactaaa gaatgatcct ccgtcaattg catgatttta
    29461 tcttcacgaa tcaaataagc tctgctatcc ccaacattgg caatcgtgaa ccagtcatct
    29521 gacagagcgg ccgctacgat tgttgttccc ataccgttat attccggctg ttcttgtcca
    29581 cgctgataaa tcagcatatt ttcttcttga atcgattgaa tgaaccattg ggcgattttt
    29641 tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta gattcgttac cgccatctga
    29701 ctggcaatgt ctccggcacg atgaccaccc atcccgtctg ctaatacagc aagcttgaga
    29761 cctgaacggc tggtaaatac actggcatag tcttgatttg tgttacgtcg tcttcctaca
    29821 tctgattgat attcaatctg cattttttca cctcattact ttttgcgcaa acaacaaatg
    29881 aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca acattttctc ttcgattgat
    29941 gattgtagca taggattggt caaaacatca atcctctcaa aatcagggtg tctttccaag
    30001 aaagcagaaa caacgtcttg gttttcttct tttaagatcg tacaagtgct ataagtcaga
    30061 ataccggaag actttaaagt tggtgcgcag ctttctaaaa tttccaattg gatttttggt
    30121 aaattcgcta attcatccgg ctgtttttta taacgaatat ctggttttcg ccgaagcagc
    30181 ccaagacctg aacaaggcgc gtctactaaa atccgatcaa actgctctgc ttcgaactgt
    30241 ttactgactt ctctagcatc catcttttga gcgtagacag cagaagagac acctagacgt
    30301 gctgcattat cttcaatgag tttgactttg tgttcgtgta catctaaagc gaccacttct
    30361 ccaccgcatg acggatctaa aaaagaagca atatgcgtcg tcttacctcc gggtgctgca
    30421 caggcatcta agacatgatg ttcaggctcg atttgcatag acggcgctac aagcatcgaa
    30481 ctttcatctt ggatcgtcat gacgccatcg tggaacaatg agctagaagc taaatgccct
    30541 tttttcgcta ctataccgta tggactgact tggctttttt cagctgtgat ctcttcttct
    30601 tgcaaaacag tgatggcctg atctctggaa agtcggcgtg tatctactcg ggcactagca
    30661 tggctaggct gatatagcga taaaccgagc ttttcagttt cttcgtatcc gatttgatcc
    30721 accagtcgtt tcgtcatcca tagaggcagg ctgattttga cagaaagtct ttcgatagga
    30781 tcagcaatgc cttgcagatc tggtgctcct tgacgctgat aattcctcaa gacaccattt
    30841 acaaatttcc ctgtcccagg atttcctttt acttttgcga tttcgaccgc ctcgtttaaa
    30901 atagcatgtg cagggacttt atctaaaaat tctaattggt atacagaaag ataaagaagc
    30961 gtcttgaccc agtcatccac tttttttgct ttagcgatca atggtgcaac gtaaaattcc
    31021 aatagtcgct gatggctaat cgtaccatag atcaattctg taaataaacc cacatctttt
    31081 tgatttagct cgctttgttt gatcatctca ttcaacaaaa gattcgagta ggcgccaccc
    31141 ttgttcactc gctctaatgt cagtaatgcc atatatcgta ctgatttttt cattttttta
    31201 gggatttttt tcattatttc acctgctgac caatctgaac ttgctgaccg cttccgttaa
    31261 ggaaatcatg tacggcctgt tttcctttac cagctggctg taattcattg atggctaaga
    31321 cggtctgttt accgcaagca atccatagat tttttttgtc tttccggatg atcgtaccag
    31381 gtgctttgtc tgtagtttct gccaatactt caaccgataa taatttccag cgtatgccct
    31441 cataggtcgt gaatgcaatt ggccaaggac gcattccgcg tacttggttg tcaatttctt
    31501 ctgcagtttt atgccaatcg attgcttctt gttcccgtgt gatgtttggc gaaaaagttg
    31561 cttctgactc gtcttgaggt actggttttt gtccatccaa aatattgggc aatgtttcta
    31621 gcaatagctt tcttcctaaa aggctaagct tttcaaacat cgtccctaca tcatcttgtt
    31681 tagtgattgg tatgctttct tgcgcataga ttccgcctgc atccattttt ttgatcattt
    31741 ccatgatcgt tacaccagtt tctttctccc cattcatgat ggcataatga acaggcgcac
    31801 ctccccgata tttaggaagt aaagaagcat ggacattgat tgcccctaat tttggtactt
    31861 gcaataactt ttcaggcaag aattggccaa atgccgcagt gacgatcaca tcaggcacca
    31921 attcaatgat ttcttccatt tctggcgaac cggagatttt ctctggctgc agcacacgga
    31981 taccgtgctt cagagccgct tcttttaccg gtgtcggcgt aattactcgt ttccgcccaa
    32041 ctggacggtc gggctgtgtt accactgcct ggatctcgta acctgattct atcagccctt
    32101 caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac tagcttagtc atcaagatgt
    32161 tcctccatat attcttcaag ttcttcttcc gggatctgct gtatcatttt ctcaatgaac
    32221 aagataccgt ctaagtgatc gatttcatgc tggaaggcac gggctaaata gccaaaagcc
    32281 gtcacttcta tctcttctcc gtctcgatca taataacgca cggtcacttc atctgctcgc
    32341 ttgaccgttc catagacatg cggaatactg agacaacctt ctacgtcaag gctttcacct
    32401 tttgcttcga tgatttctgg gttaatcagt tcaaatttct cgccttcatc tacttcgatc
    32461 acagcaattc tcttgttttg tcctacttga ggagcagcga tccctattcc gtcattcgca
    32521 atcattgtct catataaatt gtctaataaa gcgattgtct cgtccgtgat tacatcgatt
    32581 ggctgagcag ttctttttaa tttatcatta gggtggatca atataggata gcgcataatt
    32641 tggccctttc ttaaataaag tttaatggtt cgttgtcaat tgccacaaaa agaccttgtc
    32701 gctgttcttt ctggcttcca ttcaaaattt catccaaaac atgaggaaga ttcggttcat
    32761 gtttatattt gatgattagt tgataataat atcggttttt cactctcaaa atcatgctgg
    32821 gcgttggccc taatagcaag ctttgagggc ttaacacttc ttttagctga ttggcaatct
    32881 gaaagatttt ctttgccgct acttgttctt caggatggct ggcggttatt ttcacagtaa
    32941 agtaataagg aggatagcct ccacgatgcc gtacatgcat ttcctgctga taaaactgct
    33001 cataattttg ttttttagct aattctatgg cgtaatgttg aggattgaat gtttggatca
    33061 cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga aacttgggta agcaattgga
    33121 atgtatgctc actagaacga aaatccggta aattcaggga ggtatcagca ttcaacaccc
    33181 caactaatgt cacttctgga aagtccaaac ctttagcaat catttgtgtc cctaataata
    33241 tatctgcttc ttttgcaccg aatttttgta aaatctgttc gtgtgcccct tttcttcgag
    33301 tggtatctac atccatacgc aaaatcctag cttgcggata aagttctcgg agttcctctt
    33361 ctactttttg agtacctgtt ccatagtagc ggattttatt gccgccacaa ttaggacagc
    33421 gattgggaat aggttcttca tgtccgcaat aatggcagcg cattgaacgt gtatccatat
    33481 gcagtgtaag agagatatcg caattaggac atggaagaac aaatccacag tccctacaca
    33541 tcacaaaaga cgaatatcct cgacgattga gcagtaggac agtttgttct tttttgtcta
    33601 atcgattttg tattttttcc tgcaaattag cagaaaaagt agaggtccga tgattctgaa
    33661 actcttcacg catatctacg atctcaatag ctggaagctg tgcagctgct ttggctcggt
    33721 ggtttaactg caataactga taaacgcctt tttgagctcg tgctcttgat tctaaagagg
    33781 gcgtcgcgct tcctagaact attgggcaat gatgatattt actgcgccaa attgctagat
    33841 ctctcgcatg ataacgcggt gtctcatctt gtttgtaact tgattcatgt tcttcatcga
    33901 taatgatcag cccgatattt ttcagcgggg caaaaatagc agatcgtgcg cctactacta
    33961 cttgcgcttc ttcccgctca atcttgcgcc attcatcata tttttcacct tgggaaagtc
    34021 cgctatgtaa gaccgcaact gattctccga agcgtccttt gaatcgctcg accatttgcg
    34081 gagtcaatgc gatttcaggc actaacataa tagccgtttt attttctgac aaaacatcag
    34141 cgattgcctg aagatatact tctgttttcc cgctgccagt gattccctcc agcaaaaaga
    34201 cctgatcttt ttgctgctga cctgctgtga tgatttgttc gacagcattt ttttgttctg
    34261 cattcagttc caatgccgtc gtttggtcaa atactcggtc tttataaggg tcgcgataac
    34321 gttctgattc tataaactcc agccagccct tttcttttcc ttgattcaag acagcagtac
    34381 tgattccttt atttttgaaa taagcaattg gttcttcttg gctcagttca gataaacaca
    34441 aaagaagttc tttttgtttc ttggcatttt gccgtaattt tgcccattct tcttcgattt
    34501 gcttttcagt taacaaagaa cggaccaaac gaatcgtttt tactttattt cgtgtatgga
    34561 cttcatattt gatatctact aattgctctt ggcgccagcg aatcaattga gaaaggctgc
    34621 cttcttcttc tgcttccttc caggaaatca cttctcgctg atcaaacaat acctgaactt
    34681 ctggtgtgtc tgccaaagga tagatcaatt tatcatattc tgctttcatc acactcggaa
    34741 gcatcgtttg tagacaagtg attttgaatg cgtaagtaat ttttttcata taatcagcta
    34801 atgaaagtaa ttcttcattg accaccggag caaggtctag caaacgaata actgccttca
    34861 aagatggatt agctgattct tctgattttt ggatagccat aacaaatcct tggacatgac
    34921 gattgccatt accaaaaggg acttctactc gcatcccgac ttgcaaagct gtttccattt
    34981 cagagggaac caaataagtg aatggctgat cggtctgcat agtaggtacg tccacaatga
    35041 cttcagctgt taatgtcatg gttcgtttcc tccttttttt gtcttttctt atcaagcgac
    35101 aaaaagttcc ttgcctattt tactagagat cgatggattt gtcttatctt ctgccaaatc
    35161 ctaacaaaaa attaacagca gataagaaaa aaaagaactg tgtcaaaaag ttcagacaca
    35221 gccttttcta ccagacaata agtggcgaat gtttgaaaag gaaacagctg ggtttgcttt
    35281 tctttttaga tgttcgctct atttttcttt attacatttt ttgctcttca cggatacgtg
    35341 attccaattc gtgttgttca cgctcttttt gtgcttgacg ttcctcttct tccatcttca
    35401 aacgttcacg ttttaattca ggatgtggat cattgactac atttcctgca tcgatttctt
    35461 ctaaagcttg accgacgctt tttactgaat caaaagaatc tagagtaggc tgtgcacttg
    35521 catccaattc atgcgcgcgt ttacttgcta aaatcactag agaatatttt gaatttacac
    35581 gatctaatag tgagtcaatt gaaggtttta acatcataag actacatctc ctttaacatt
    35641 ttaatatatt taccgattac gcgatctact cgaaaatgtt cactggcaat aatgtttttg
    35701 attcgttcaa ctgctagcgg cacttcatca tttacaacag catagtcgta aagagccatc
    35761 atttcgattt cttcttttgc aactttcatt cgttcttcga ttacttcatc tgcatctgtt
    35821 cctcggccta cgatccgtga ttttaattcc gctaaatctg gcggagtcaa aaagatgaat
    35881 acaccatcgg gcactttttc tttgacttgt tttgctcctt ggacttcaat ctccaagaaa
    35941 acatcttttc cttcatccaa tgtcttgttg atatatgaca aaggagttcc ataataattc
    36001 ccgacatatt ctgcgtattc cagcatctct ccagcttcga tcattgattc aaactcttct
    36061 ttgcttcgga agaaataatc aacgccttcg acctcccctt cacgcatttt acgggtagtc
    36121 attgagatcg aatactgaaa atcattatcc tcactctcaa aaattgcttt tcgtactgtg
    36181 ccttttccta caccggatgg accagacaac acgattaata atccacgctc tgacatgacg
    36241 acgtcctttc aactagtctt tttgtactta tcgtttattt tgcacttttt ttctgcaact
    36301 ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa agctaccctg taccaagagg
    36361 tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacataaac agcttctcat
    36421 gtttttcgtt tggttggggt tattatcgca gatgcaagct tttgaacgcc gtgtataaga
    36481 ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa ttttgtgaaa atagtttctc
    36541 acatttttgc ataaatttgg cttaata
//
LOCUS       DASHFF010000024        34691 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.024, whole genome shotgun sequence.
ACCESSION   DASHFF010000024 DASHFF010000000
VERSION     DASHFF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.024"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(59..1612)
                     /locus_tag="U2I75_001318"
     CDS             complement(59..1612)
                     /locus_tag="U2I75_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1696465.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            1760..3049
                     /locus_tag="U2I75_001319"
     CDS             1760..3049
                     /locus_tag="U2I75_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1696466.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEAAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(3175..4254)
                     /locus_tag="U2I75_001320"
     CDS             complement(3175..4254)
                     /locus_tag="U2I75_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1696467.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4363..4803)
                     /locus_tag="U2I75_001321"
     CDS             complement(4363..4803)
                     /locus_tag="U2I75_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1696468.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4860..5270)
                     /locus_tag="U2I75_001322"
     CDS             complement(4860..5270)
                     /locus_tag="U2I75_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1696469.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5451..5885)
                     /locus_tag="U2I75_001323"
     CDS             complement(5451..5885)
                     /locus_tag="U2I75_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1696470.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(5985..6833)
                     /locus_tag="U2I75_001324"
     CDS             complement(5985..6833)
                     /locus_tag="U2I75_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1696471.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            7222..8988
                     /locus_tag="U2I75_001325"
     CDS             7222..8988
                     /locus_tag="U2I75_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN1696472.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            9090..10349
                     /locus_tag="U2I75_001326"
     CDS             9090..10349
                     /locus_tag="U2I75_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1696473.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            10496..11797
                     /locus_tag="U2I75_001327"
     CDS             10496..11797
                     /locus_tag="U2I75_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1696474.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            11800..12651
                     /locus_tag="U2I75_001328"
     CDS             11800..12651
                     /locus_tag="U2I75_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1696475.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            12664..13479
                     /locus_tag="U2I75_001329"
     CDS             12664..13479
                     /locus_tag="U2I75_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN1696476.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            13630..14796
                     /locus_tag="U2I75_001330"
     CDS             13630..14796
                     /locus_tag="U2I75_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1696477.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            15026..15673
                     /locus_tag="U2I75_001331"
     CDS             15026..15673
                     /locus_tag="U2I75_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1696478.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            15757..16701
                     /locus_tag="U2I75_001332"
     CDS             15757..16701
                     /locus_tag="U2I75_001332"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN1696479.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            16836..18287
                     /locus_tag="U2I75_001333"
     CDS             16836..18287
                     /locus_tag="U2I75_001333"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN1696480.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            18308..19144
                     /locus_tag="U2I75_001334"
     CDS             18308..19144
                     /locus_tag="U2I75_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1696481.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            19144..19803
                     /locus_tag="U2I75_001335"
     CDS             19144..19803
                     /locus_tag="U2I75_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1696482.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            19929..20618
                     /locus_tag="U2I75_001336"
     CDS             19929..20618
                     /locus_tag="U2I75_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696483.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            20624..21580
                     /locus_tag="U2I75_001337"
     CDS             20624..21580
                     /locus_tag="U2I75_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN1696484.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            21624..22136
                     /locus_tag="U2I75_001338"
     CDS             21624..22136
                     /locus_tag="U2I75_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1696485.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(22392..22940)
                     /locus_tag="U2I75_001339"
     CDS             complement(22392..22940)
                     /locus_tag="U2I75_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1696486.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(23029..24210)
                     /locus_tag="U2I75_001340"
     CDS             complement(23029..24210)
                     /locus_tag="U2I75_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN1696487.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(24446..25954)
                     /locus_tag="U2I75_001341"
     CDS             complement(24446..25954)
                     /locus_tag="U2I75_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN1696488.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            26113..26304
                     /locus_tag="U2I75_001342"
     CDS             26113..26304
                     /locus_tag="U2I75_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696489.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            26507..27424
                     /locus_tag="U2I75_001343"
     CDS             26507..27424
                     /locus_tag="U2I75_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN1696490.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            27417..28490
                     /locus_tag="U2I75_001344"
     CDS             27417..28490
                     /locus_tag="U2I75_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696491.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            28638..29156
                     /locus_tag="U2I75_001345"
     CDS             28638..29156
                     /locus_tag="U2I75_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696492.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            29137..29529
                     /locus_tag="U2I75_001346"
     CDS             29137..29529
                     /locus_tag="U2I75_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN1696493.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            29605..30540
                     /locus_tag="U2I75_001347"
     CDS             29605..30540
                     /locus_tag="U2I75_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN1696494.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            30673..31452
                     /locus_tag="U2I75_001348"
     CDS             30673..31452
                     /locus_tag="U2I75_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1696495.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            31445..31762
                     /locus_tag="U2I75_001349"
     CDS             31445..31762
                     /locus_tag="U2I75_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1696496.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            32006..33616
                     /locus_tag="U2I75_001350"
     CDS             32006..33616
                     /locus_tag="U2I75_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1696497.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            33773..34621
                     /locus_tag="U2I75_001351"
     CDS             33773..34621
                     /locus_tag="U2I75_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1696498.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        11467 a         6170 c         6716 g        10338 t
ORIGIN      
        1 ttgatacata aatccggagg ttgttaataa gcattcggat ttatgtatct tatttggtct
       61 aggaagtaac ttgattttct tttttcgatt gatccatgag tgcttcttct tttaattcaa
      121 agatatattc tgagatcctc ataatcactt ttttccagtc ttctggtgtg atgattcctt
      181 ttaaaatata gatttttttg atatgagaag gcaattgttt caaagggcag tcgctgataa
      241 cgatgttgca gttctccata aaatcatttt gtattttgat cgattcattt gaaaaaattt
      301 gttctatctt aacgatcaga tctttactga tataaaaatc acgcgtatag ttcacaccaa
      361 tcacaattcg cggctgtttt ttgtagcgat catacaaatg tactaggtct tcaaccatcc
      421 attgcacatt ttccttttct aaaataactg gtaacaagtg ttgattttgt tctctgaaat
      481 ccatataaaa ttgttcaatg gcggtttgga cttctttcaa cacgctatgt tcttgataaa
      541 gcatgaaagt aggaaggggc gtatttggtt gattgaagtt aagaaatcca ttataaagtt
      601 tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa
      661 atacagtaca aaaggactcc accatttctc ttgaataaag aacatgagga acgtttgctt
      721 tttttaaaag tttgtattgc tccaagcttg tttcttccga ttcgattttc gtgatagaaa
      781 gtcgagctaa aatattcact aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca
      841 aaatatctac agagggatca ataaaaaagt caatatattc atactctgtg aattcttttt
      901 gtacgtcttt cacagaagtt gcaggaaaat tctctttaca tatttttaat agaaaatata
      961 atttatctat cataccgcta tttagtcggt caagtacttc ttttttaaaa aaagtattca
     1021 tcataaaagc cactacttgc ggatcttcat cataaaattc tgtagcagta cttgaaaaga
     1081 tgttccaata aatatctaat gctgcatatt gaatttgtac ctcttttcca gaaaaagact
     1141 ttctgccatt attagaaaag gagatagaca aaccgtacaa tgctaaaaat gcattaatct
     1201 ttttcatttt cgtatacaaa taagaggcac tatagtttgt ttgctgactt aaactgataa
     1261 tcgaatttgt ctgacagctg aacaaagaag tcaagatctg ataactgact gattcgttgc
     1321 agtatcttcc aaatagacga taatagcagt aagccggatc aattccttca aaattcaact
     1381 ttaaaaaagg tttttgctta tcaatatgca gttctgttag attgagacgc tcaaagtctt
     1441 gttctaattc tttgatgtat agtaaaaaat ttgattcaga catatggaaa tgatctgcta
     1501 aatattgtgt agtaataaag aaacgatgtt cagataggaa aagaaaaata tctaattttg
     1561 tattgctttt tttcttcaag agaaacgttt taatcgcttc ttgagtttgc atgaaagagg
     1621 ctccttttta cttaaattgt taaaacttga aaatattact gtttttttca taaatttaga
     1681 caaaaaaaca gtaaacatat aggtagaatc tctactatct tatttcttgc atacaaaaaa
     1741 atcaaggagt gagagagtta tgagatattc aaccatctta atatcaacat ttgcagcctt
     1801 aagcttatcg cttactataa atcagacagt ccacacagag gagatagaaa cagccaaatg
     1861 gacagaaacc aagtgggcca acgtcaatcg aattaccttt caggatgaat tttcatcctt
     1921 caattcagga gtcgcaaaat taagaaaagg acaaacagcg accatcaaag caaaaattga
     1981 ctattcagga gacttagcac ctatactaca atcagctgtt acatttgaga atgttgatcc
     2041 cgctttgtcg attggctatg acgagaacac tttgactatc caacacaagc aagcgatatt
     2101 taccttcacc gttacattaa atcaggactt gttaaaacca gcgcttttta ctgtcaaggt
     2161 atcagatgga ctaccgaacg atcatcatca tttaacgccg tatagtcagc ggcagacaat
     2221 tgagcaagat tcagctatcg actcaggagg agatcttgtt gaagaaccaa cagataaacc
     2281 agaaaatgaa aataagccag aagtaccgcc tacagaaaat ccagacggag aacagaaacc
     2341 ggaaatagaa cctggagaag aacccgatac agaaactcaa ccagagccag acaatgaatc
     2401 aaaacctgaa ataacccctg gagaaaaacc agatgtagac ccagaagaaa aaccggatgt
     2461 gactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa atccagacac
     2521 agataatgaa actgaaaatc cagaaaaacc agaggcagcc ccagaagaaa aaccagatgt
     2581 aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa atccagacac
     2641 agataatgaa actgaaaatc cagaaaaact agaggtagat ccagaagaaa aaccagatgt
     2701 gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa
     2761 aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa ttctaaaaat
     2821 aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat cagctgaaac
     2881 cttaaaaagt agcaaggata caacctttgc tagtcctgaa acgaaaaata aacagctacc
     2941 aaaatccgga gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat
     3001 agcaacaatg ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa
     3061 tgtctgtgaa cgcatttttc aaataaaaag aaatatccag aaaacgaccc caaaatacca
     3121 gattgaaatc tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg
     3181 gatatctgtt tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa
     3241 gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac
     3301 aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg
     3361 attcagcaca taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac
     3421 atctcggatg cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac
     3481 acctgctact gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg
     3541 aaagccaaat acagccaagg cgattgtagt gagcaacgtg tttaaaagag cgataacaaa
     3601 ttgagcttct aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc
     3661 ttgaaagaac caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt
     3721 cttttcaatc ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt
     3781 tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa
     3841 atcgtttcgc tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg
     3901 atgctcgtaa aaatcaataa cggactggat catatcaaat gtttgatgaa taagcatcgg
     3961 gatatacttt gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc
     4021 taaaacacct gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa
     4081 ataagtaaag ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac
     4141 aaaaacgaca aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg
     4201 ataaacggtc aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg
     4261 atggaatatg aaaataagaa agagaaagag caaaaagaaa aaccgctaaa tgacgttatc
     4321 ttatgagttg gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc
     4381 ttgatgaaaa ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg
     4441 ttcaagtgga agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc
     4501 agcaccttga ggagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg
     4561 attaatataa tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag
     4621 acacaaatca tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat
     4681 cacaagatac tgggtatagg taagattcag cggcttgagg agggaagtgt agtgtctaat
     4741 gatttcctta gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc
     4801 catttttcca cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc
     4861 tattcactaa ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga
     4921 cacactttat gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata
     4981 gatccttcaa tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact
     5041 tgtgcactga cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca
     5101 ctgttaaaac atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg
     5161 acgcgtttac caggagctgc aatattcaat tcgaatgtac gatcgggatt atgaacttct
     5221 ccagagcgtc cgcctgtgtt gatcattttc gtagaataca tttttttcat aaaattacca
     5281 cctttaatta tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa
     5341 tggattggca taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc
     5401 aaacatatac aaaaaaatat cagagacaaa aactaatctc atcataaaca ttacatatat
     5461 ttctcaaaaa gttgttttct ttcttcaaaa cttacttcac gatggacctg atgaagcatc
     5521 cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc catcatttct
     5581 gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt gtcctcgata
     5641 tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg agcagtcaaa
     5701 ccaagagacg tgttttcatc cagcatatgg aatctcgtgc catacaaggt aaaaatgact
     5761 tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc aaagattgtt
     5821 tgatacagtt ctagtgcttt caaactatca tttaatacaa aatctatttc cgttccttta
     5881 tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt attataccag
     5941 ttaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc gattggatga
     6001 taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa ggcacttgga
     6061 tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac aacgatcaat
     6121 aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt aaggccacgt
     6181 ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact catgaccata
     6241 acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga agtgattgca
     6301 atagataaaa tagcataaag aaactcttct attccaaaaa caaacagatt aagagaaaca
     6361 acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata ttttttaaaa
     6421 atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa aatcgcaact
     6481 cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga agtaatcatt
     6541 gtttctggaa ctattgcaat gattaaagga aaaaacaagc ttccgaaaag tactttaaaa
     6601 aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt caacactaaa
     6661 ataactatag ctcggtcaaa tcctaaaaca gcttccagta atattcctag accgctgaca
     6721 cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc taatatgaag
     6781 agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt catttatcca
     6841 cttccttaat tcattaataa tactcttatc ttattataac ttacttttca ccttaagtta
     6901 actttaatta ataaaaaaat taggctagtc taaattatta tgtttcattc ttctagaata
     6961 tacttctacg ttaaaaactg ttaaaaaact tttactttag aaaattggag aaattgatcg
     7021 cttaagataa attcaggaca ctaaatagta caggaagtag attaaaactt ttaaaagttt
     7081 taagtctatc tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa actgttaccg
     7141 ataacatttt tgaaagcgtt gtcttttgtt tctctatcac ttatagtgat gacgtcatat
     7201 taaaacacaa ggaggaaatc aatggataca gctgcaattt atcatcgacc ggaaagtgaa
     7261 tatgcatttc tatatacgaa tacaagattt cgtattcggc ttcgtactag gaaaaatgat
     7321 atcaaaaaag tatatgtgct ttgtggagat ccatatacca taacaactga aaaatggtat
     7381 caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta ttgggaaatt
     7441 gaagtgacga gcgaaacaag gcgcttacag tatgcgttcc atgttgttgg ggaagataac
     7501 acggactgtt tttacggtga tcaaggaatt tttccttatc gagaaaatgt gattactgaa
     7561 ccaaattttt attttcgaat accttatttt catcaaatcg atcgattcac tattccagaa
     7621 tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa cggggacaaa
     7681 gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag atccagaccg agaagacttc
     7741 ttcgggggag atttgcaagg tgtgagggat catttggatt acttgacgga ccttggcgtc
     7801 aatggcatct atttttgtcc catatttaaa gccacatcca atcataaata cgatacgatt
     7861 gattattatg aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa cttgattgat
     7921 gaagcacata aaagagggat acgtatcatg ttcgatgctg tattcaatca tatgggtgtc
     7981 cattcaccac agtggcaaga cgttctcaaa aacggagaaa aatcgatcta taaagactgg
     8041 ttccacatcc gttttttccc agtagattct tatcagatga ctgacttacc agaaactgct
     8101 gaaaatatcc catatgatac ttttgctttt actccattca tgccgaaact aaatacagca
     8161 aatccagaag tgcaaaaata tttgctggat atcgcaactt attggataaa agaattcgat
     8221 attgatggat ggcgtcttga tgttgctaat gaagtcgatc atcatttttg gaaaaaattc
     8281 agagaagcag ttacagaaat caaaccagac atctatatcc ttggagagat ttggcattcc
     8341 tctcaagctt ggctgcaagg ggatgaattc catgcagtga tgaattatgc attcacagat
     8401 tcgattaaag attattttgc aaaaaagaaa atcacagctt cacaaatggt cagtggaatg
     8461 aatcatcagc aaatgcttta cagagatcag gtgaacgaag gaacattcaa cttattagat
     8521 tctcatgata ctgccaggat cttgaccctt tgccaaggta acaaagaatt gatgaagtct
     8581 gtgatggcct ttatgtttct tcaaaaaggg tctccttgta tctattacgg gacagaaata
     8641 gggatgactg gtgaagatga cccggattgc cgcaaatgta tgatatggga gaaggaggaa
     8701 caagatctag agctattcgg ttttataaaa gaacttgttt ctctaaggaa acagttgtct
     8761 aagattattt ctgaaggttc tactcagtgg ctaatagtat atgatcgtga agataagctc
     8821 tatttcacac gggaactaga gggacagatc atctatgttt actttaatca gtcaaaagaa
     8881 ccttgggtag tagaacagga aaatgaggtg attcttagtc aaaattgtca gttgttagaa
     8941 gacgggaaag cagagatacg acaatacgga tttttgatca tgtgttgaaa actgttaccg
     9001 gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc attataataa gggtgtaaca
     9061 gcacgttgaa tacacaggag gaaatgaaga tgaaaaaaag aagtgtgaaa cagttgggtt
     9121 tagggttaat ctctttagga atcttaggcg gattagcggc ttgtggcaat ggtaactctt
     9181 cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat tctggatata
     9241 aagattatat aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg aaaatcaagc
     9301 tagtagaaaa ggacatgttc gatcagttgg aatcattagc gttagatggg cctgctggaa
     9361 agggacctga cgttatgatg gcagcatatg accggatcgg tgcgttagga cagcaaggac
     9421 atctggcgga agtaaaactg ggaaatgaag cagcttacga tgaaacagac aaagcccaag
     9481 tgacttatga tggaaaaatc tacggagaac ctgcagtaat cgagaccctt gttttgtact
     9541 ataacaaaga tttagtcgat acagcaccat caacgtttaa agatttggag aatctatcca
     9601 aagacagtcg ttttgctttt gagtcagaag caggaaaaaa tacaggattt ttagctaaat
     9661 ggactgactt ttattattcc tatggtttaa ttgcaggtta cggtggctat gtatttggtg
     9721 atgatggcac aaatccttca gatatcggtt tgaataatgc aggtgctgta gaaggaatct
     9781 cttatgcaac agattggttc aaaaatgttt ggccaaaggg aatgcaagac ataaaaagtg
     9841 caggagactt cgccagccag caattcatgt ccaataaaac tgcagcaatc attgacggtc
     9901 catggcaagc gcaaacatac aaagaaaaca atatcaatta tggtgtagcc aaaattccta
     9961 ctctaaataa tggacagcct tatcaaccgt ttggcggagg aaaaggatgg gtagtcagca
    10021 attatgctaa aaacaaagac ctctctcaaa aatggcttga ttatgtaacg aatcaggaaa
    10081 atcaagaaaa attctttgaa atggtaaatg aaatccctgc aaaccaacaa gctagggaaa
    10141 cagcaaaggc taaaaacgat gaattaacaa cagctgtgat tgaacaatat gaaacagctc
    10201 aagcgatgcc aaacatccca gaaatgggcg aagtttggac aggtgcagaa aacttgatgt
    10261 tcgatgcagc atcaggttca aaaacaccaa aagaatccgc agatgaagca gtaaaaacaa
    10321 tcagtgaagc catagaacaa aaatactgat taaaatgata accgtccatt tttataaaaa
    10381 atggaacagg tgcttcagat aaaagtggca agagcgagat ggaaaattat cttgctcttg
    10441 ttgccattat cttaaaagca gtttgcggtt tagcaagttt agagggggca ttcagatgca
    10501 gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac ttattccagg
    10561 gatggggcag tttgtaaatg gccaagcttt taaaggtatc gtttttttcg ccatattcgt
    10621 cctattcatt ggtcagatgc ttctgggcgg atttcatgca ttggtaggat tggttacttt
    10681 gggaagtata ccaatggaag atcattcatt atttattttg attgaaggca ctttgcaatt
    10741 catcattaca atcattttct tgatttttta cggtttgaat cttttagacg cttatcgagt
    10801 agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag tcatacataa
    10861 tataggtgat gaaggttttc cttatctatt gacacttccc gcttatcttc tcatgatttt
    10921 tacgatcatt ttcccagtcc tagttacatt gttcactgca tttactaatt atgacttcaa
    10981 tcatattcca ccagccagtt taattgattg ggtaggggct aaaaactttt ttagtatctt
    11041 ctttttaagc tcatatcgta atactttttt tgcagtcttt tcgtggaccg tgatttggac
    11101 tttatgtgca acaactttgc aaatcaccct tggtattctc acagcggttg ttgctcacca
    11161 gaaatttatt aaattcaaac gtattttcgg tatcattttc ttattaccat gggcggtccc
    11221 agcttttatt actatcatga gtttttcgaa tatattcaac gatagcgtag gtgccatcaa
    11281 tactcaagtg attcctttca taaaccagct tccttttgta gatatcagtg caatctcttg
    11341 gaaaacggac ccgaactgga cgaaagtagc aattatctta gtgcagggct ggttaggatt
    11401 tccctatatc tatgtgatgg ttacaggaat cttacaatcg atttcagaag acttatatga
    11461 agcagcaaaa atcgacggag caaatgcatg gcagcgcttt tcaaacatca cgcttcctgc
    11521 tgtcttccta gtggcagcgc caacatttat tacacagtat acggggaatt tcaataattt
    11581 ctccatgatc tatctgttta acaatggtgg tcccggaagc gtaggcggag gagctggttc
    11641 tactgatatt cttatttcct ggatctataa acttacgact ggaacttctc cgcagtattc
    11701 tatggcttca gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt cattgctggt
    11761 atttaaaaag acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt tcattcgagt
    11821 gctcgattcc aaaaacgact ttctcacttt tttacttact tttttctttt aatactgtca
    11881 gtcattatca tctatccgct tttgatcaca gcaagtactg ccttcaagtc aggaaatatt
    11941 gcagctttta gtttagatct ttcaagtgac tggactttag gaaactttac tcggctattc
    12001 agtgaaacac tttacgggac ttggtataaa aatacattga tcatttctgt cctaacaatg
    12061 atcgtacaag tgactgtagt aactttggcg ggctatactt atagtcgcta tcgttttatc
    12121 ggccgcaaat caagtttgat gttcttttta gtgattcaga tggtaccgac tatggcagca
    12181 ttgactgcct tttatgtcat ggctttctta ttagaagcgc tcgataagta ctggttcttg
    12241 acaatgatct atataggcgg tggaattcca atgaatactt ggctgatgaa aggatacttt
    12301 gacactgtac caatagattt agatgaatct gctaaacttg atggggcagg gcatttcagg
    12361 atttttttcc agattgtatt accgcttgtc aaaccgatgc tcgccgttca agcattatgg
    12421 gcttttatgg gaccttttgg ggatttcatg ttagctaagt tcctgcttcg aacacctgaa
    12481 aatcagactg ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa ccaaagagtc
    12541 tctctttttg ctgctggggc aattttgatt gcactaccga tttgtctatt attcttcttt
    12601 ttacaaaaga actttgttac cggcctgaca tctggcggaa caaaaggata ggagcagagt
    12661 atcatgcaaa catttccgtt aaactatttt tccgcattac gtacgccaac tcaagtgttt
    12721 gcgggacgca aactattatc atggcctaaa tttttcttga tatttgtatt tttagtttcc
    12781 ttgatggtca tgcctgtgac tcttttttat gccaatcaga tccaagcgat tccattagaa
    12841 caatttctat ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt ttctgaactt
    12901 gaattatctg aaactgggtt acaagcatcg caacaaacga tagctgtgac ccctgaaatt
    12961 ttggtaggtg tgtctttatc tgagaaacaa caatccgatc atggaacttt tattgatttt
    13021 gagaaggaac aatgggtgat ccaacaaaag gataaatctg gtatacgccg ctatacgatg
    13081 aattatagcc catctttcca accagattct gtacgtacgc ccgaagattt ccaacgattt
    13141 ttagaaagag aattttatgc cagtaatcgt ccaacaatca ttttatctta tagtttaagt
    13201 cttggtcttg tcctatttgt catgacgagt ctcattttat tcggggcttc ctttttcttg
    13261 tggatgacaa gaaaaagcca actttcatcc attcatacat tcaaagagag tgcaaatttg
    13321 atgctgaatg ttatgggaat aggttccatg gcagcagcag ttgtagggtt tatccacttt
    13381 gactttatct tgatgctaag tgtccaaaca acgataactg tcttgctgct tttatgggtt
    13441 tttgtcaaaa caagattcaa agataagcgt gtcgaatgac aagtttaagc gatttatgcg
    13501 acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct gaaaaaatta
    13561 aacttcttag ttgattcttt tcttttttag tattaacgta atagggtagt ttttttaagg
    13621 aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc tttctcgctc
    13681 cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac ggacactcct
    13741 ccccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac aagtaacgct
    13801 tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg aagtatccaa
    13861 gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa agattatcta
    13921 aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga tatccatgcg
    13981 cattatgact taggtaacga cttttataaa ctttggctag atccaacttt gacttattct
    14041 tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa taaagtccat
    14101 catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat cggttgtggc
    14161 tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac tgggatcaca
    14221 ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca tttagaagat
    14281 aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt tgatcatatc
    14341 acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta tttcaaagtg
    14401 gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag ccgacaacaa
    14461 ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta tgtccctggt
    14521 gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga tttggaaagc
    14581 ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca tcaagtccaa
    14641 gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct atatttacaa
    14701 gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt gcttgttcaa
    14761 cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta ctgtttaaag
    14821 actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt tgtctaactt
    14881 ctaggattgt ctctaaagct tagtttcttt ttctttatat tttcataata aaagtttggt
    14941 tctttaattt ttaaacatga cagaaaattt tttcttcgtt atacttgtaa tagaatacta
    15001 ttttataaca aaggggatcg aatcaatgaa aaaaactgtt atttatacta gtttatttgc
    15061 agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc
    15121 ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag caagcacatc
    15181 ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata tcaaagtgtc
    15241 tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa ttactagttt
    15301 ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg atgatcaaaa
    15361 agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga atccagaaac
    15421 tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa
    15481 attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc
    15541 aacagattgg gatttggata aagatttagg tactacttat tgggaagtca aagtaaaaga
    15601 cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga
    15661 acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta gtcatgatac
    15721 actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac gacctttgcc
    15781 aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat gactacccag
    15841 gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag ctgtacgatc
    15901 aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg gagtatcaat
    15961 tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct tgcgtatgaa
    16021 aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat gagtgtccag
    16081 gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat cactgaatta
    16141 aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt tgaggccact
    16201 tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat caaattgcct
    16261 ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca gtttccattg
    16321 acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac agaacaagaa
    16381 gcagtagtca tcaaaccaaa agaagggttc ggcggcattg gcggagagta tatcaagccg
    16441 actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat ccaaatcatt
    16501 ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg tggcgcaagc
    16561 atgttacaga ttggtacaga gcttcataaa gaagggccgg aaattttttc tagaatcata
    16621 aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga atttaaaggc
    16681 aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc gtcttttttt
    16741 ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt tagacgaact
    16801 tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa
    16861 aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata cagaaaaaaa
    16921 taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt
    16981 cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc ccatttcctt
    17041 taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct cgtttatttg
    17101 aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg ataggtctag
    17161 ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca gacccagtca
    17221 tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga tcaagtggag
    17281 gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt gatggcggtg
    17341 gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct tcgcgaggga
    17401 cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt gatttcggct
    17461 tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt atccattccg
    17521 gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa aaacggttga
    17581 agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag gcagtacttg
    17641 ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca gagattcctt
    17701 acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc gcagaaacag
    17761 cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg
    17821 aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct acttacatcg
    17881 attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat caagaatacg
    17941 atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa gatttgcaaa
    18001 gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta gaaggtttag
    18061 acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca cagttggcta
    18121 atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa aacatgcctc
    18181 taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa atcggctatc
    18241 tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag aaacaaggag
    18301 aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt ttattttaag
    18361 cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg tagaaaaaaa
    18421 gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt ataatgatca
    18481 aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac ggttaaatgt
    18541 caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt caaaaagaga
    18601 atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa aattctccct
    18661 ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag atgattcagg
    18721 aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca atactagcta
    18781 tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg ccacgaatga
    18841 tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg attattatct
    18901 gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg agaacgttta
    18961 tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc tgaaaaagaa
    19021 aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa tatctgaaaa
    19081 atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt cggtgagtga
    19141 gtgatgtact ttcctctaat tgatttttcc ctcatgtgga cttcacttcc tttcgtactg
    19201 caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg aaatttggta
    19261 gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt atttattcgt
    19321 ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct gttatatttc
    19381 gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac gattaccagc
    19441 agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc aggtcagtgg
    19501 gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat cgttttgccg
    19561 caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct agtgaaaggt
    19621 acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa gatcataggc
    19681 ggtcggacat tcgattattt ttctctctat attttagttg cactgattta ttgggtcatt
    19741 tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatcca tcagtcaatc
    19801 tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt ttcttgtata
    19861 ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata tgttcggagg
    19921 tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac caatattgca
    19981 tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag
    20041 atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc tatgagctga
    20101 aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga cattataatt
    20161 attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct acaggaatag
    20221 gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct ggtacatttc
    20281 tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc
    20341 gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa
    20401 aatattttcc aaaagatcaa ttgtacaaag aattgaaaag acgttcagaa aatcaagatc
    20461 cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt caggcaatca
    20521 atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg aggcaacaac
    20581 agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact atccttattt
    20641 aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag gattgctctc
    20701 caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta aaaatgtgat
    20761 cgaattattt caaaaaaagc accatccaat cgctgtgatc caaaatcccc aagtaggaca
    20821 attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata gctctgtcgt
    20881 ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac ctccagcgtt
    20941 tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac agttggcagg
    21001 agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc tgcccgaaaa
    21061 caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt atgcagataa
    21121 aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat atattggatt
    21181 ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta gccgagcgct
    21241 tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac attttgtctc
    21301 tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag gagataaaat
    21361 gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa tgattgaatt
    21421 attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa agtggtcact
    21481 tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt ggcttaaata
    21541 ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg taaatgaaaa
    21601 aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa gaagtagaag
    21661 aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat
    21721 ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa
    21781 gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc tcaggcgtgg
    21841 atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct tatttatcga
    21901 ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag
    21961 aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc gatacttatc
    22021 cagctaatga accgatggaa aagaccatct attctgctgg attcacttat caagggatga
    22081 ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc aattgactat
    22141 aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat catatgatag
    22201 ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt ccgaaaatgc
    22261 ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc tgaaactccc
    22321 ttcatcgaga ggtcgtgaca tagcttttgt cacgatctct cggcttatcc tacgagtatt
    22381 attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc ttggaacgct
    22441 ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa ctgtgctaaa
    22501 tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc acgttttttc
    22561 aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac taccgtcccg
    22621 aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca atttcctact
    22681 gtcagtttct ttttaatttc tgctttactg gaaccatatt tatgttgagc atattggtcc
    22741 cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga aacaaaataa
    22801 taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt aatgatcttt
    22861 tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga aggaccgata
    22921 aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt gaaaaagctt
    22981 tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatttt ataccagcaa
    23041 gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa ttttcaatcc
    23101 gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac cagaaatgat
    23161 cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg ctgttgtacg
    23221 ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt catttgtaac
    23281 cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga ttccgctgat
    23341 gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc cgaatatccc
    23401 gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca tagcagcaac
    23461 cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga aaaatgtctg
    23521 ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat agggatttac
    23581 aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga tcacatattt
    23641 agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag acatagccga
    23701 tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt ttatggaaat
    23761 aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt ccaatttgcc
    23821 catcccgttg attttactta atcctagacc aacagctttc ataaataatg gaagaatctt
    23881 gatatagcgt agaataccga taatagcaga aataaataca attggcatca atacggaaag
    23941 aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa caccttgccc
    24001 agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg ctgtaccaaa
    24061 agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca caattagtgg
    24121 tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta atgtgacaaa
    24181 taaaccaata atacctaata aataactcaa ataatcactc ctattccaaa ttccgtttta
    24241 ttttacctgt tttgcaaata aaatactaat ctttagtgaa attttcatta cttttttaga
    24301 gtgaaacgca ttcattttcc attagaaaat aaagcgaaca aaataaaagg tttaaaaagt
    24361 gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg attaagctaa tagtttcgtt
    24421 ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa gggtagacag
    24481 gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt ttattgacca
    24541 tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa gcaacttgag
    24601 cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg atgcttagtg
    24661 catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca agaactaatg
    24721 gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca tttaagataa
    24781 ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact tcatctggta
    24841 attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga atcttatctg
    24901 catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg acttcttcca
    24961 taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg aagtcgcatt
    25021 ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca acggtatcat
    25081 ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga taaacagatt
    25141 ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca acatcccgat
    25201 tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc atattgacat
    25261 cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc aacggaacat
    25321 tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt tggctttcat
    25381 gatacagttg gttcaagcaa gtagcgagct ttccacttcc tgggccagga gctgtcacta
    25441 caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct tctgagacga
    25501 ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc ttgatgtttc
    25561 gtctttctag tttattgata aagactttcg tggcaggctg tccactatag cgagtgataa
    25621 cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc aagatatcca
    25681 tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct ccagcataga
    25741 cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt ttcgcatctt
    25801 cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactaacttc ccaccaaatt
    25861 ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac tgctcttcaa
    25921 tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa gtactaaagc
    25981 attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca aaaaaacaaa
    26041 gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag tttttgataa
    26101 ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca ctatatccgt
    26161 tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca attgacgttt
    26221 acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat tcatttcaca
    26281 acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc gattctgtcc
    26341 tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg gtgcagcctg
    26401 ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat ggtactattg
    26461 aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga aagagccaga
    26521 tacaaatgca aattatgagc cagacgaact agaggagacg aacgcggatc aggatacagg
    26581 acaagaatcg tcttcttttt acgatatcaa agaaaaagca agcatgaatc tttcccaatt
    26641 attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc ctgagatcta
    26701 ccgtttatac aatggccgat tgaatgaaga attaaaaaga agctcaaatg aaaatcacga
    26761 gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat ttccttttat
    26821 ggaacaagta gaaaacatct cgccaacgat gagctattac aaaatcggca cttattacca
    26881 tgatcggccc acggtcggta ttgatgcaag tctgccagaa atttttgttt tgcctaagat
    26941 cgatgaagag tgggagaaat attcccaacc ggagtcaacg gactatgaca aaaaaatcaa
    27001 tgagctagac gcaaaggtga tcactgctca gacagaaatt gaacgtttag atgaacaaat
    27061 aaaagaaatc aatcgacaaa tgactgattt gaacgacaat aaaggattct tgaatcgtaa
    27121 aaaaatcgag gaagaaattc aagaattaga gaagaagaag caagtactat ctaatgaaaa
    27181 attaggctgg cttccttata ttgagacgcc tgaaacaatc cagcagcaaa aagaagcatt
    27241 gaagcaagaa gcaagagcag accagcttcg tgcagccatc gtagaaaaag aacaaaggca
    27301 aatcagacgc tatttcggta gcaaagaggg attcggtcaa gcaatccatg aattcttgat
    27361 gaattatctt ggcaatgaga atcctaataa tcaatctaat gaaggaggtt ccgagtatga
    27421 atgaacacga tgaagcagaa tggacaaaaa atgaagaaca aacagaagat catgaagaaa
    27481 cgaacacttc attggaagaa caaacaagta atcctctgga tgacacagaa gaaatcccta
    27541 tgctagagga aacaaaaata catgaaaaag aaaaagtaac tccgacgaat acgaatactg
    27601 cagaaaacac ccatgcttac gaaataactg aaaatcccgg gtatttagaa ggaaaagagc
    27661 aagatcatca agatatcgtc agtttgattg aaacagcgga agcagaatta gcggatttac
    27721 gtatgagaaa agatttgatc gaatcagatt ttccacaatt attagaatca tatgatcgtt
    27781 ttatattgaa tgaagaacca atctccacga tcggaaagac aagcctgatc gaatacttca
    27841 cgtatgaatt actgaaaccg ttgaatgata ttggtctgga acggtcaaat tcagcttatg
    27901 acacatggaa aacaagacat ttttcgatca attatacgtt gaatggacaa aacgaattag
    27961 tcttttcctt tgtattaccg accatcgatc aacgatacca agtggaatcg atgagtctcc
    28021 taaacgtatc accagagaca atggaaatca atgtccaaga tgaccgggtg ctttctttga
    28081 tccgacactg gtcagtggat cgagtattct ctgcagggca gatcacgata ttcaatcaca
    28141 aattgaatca attattagcc catgcaagaa cacttggttt taccgttaat cagacattac
    28201 tagacaatac gaagccactt cgtttgaatc ttcagagcga atttgaattg acgaatgaag
    28261 tattggatga tatttttatc gtggcaatga aaaatccatc ctacgatatg gaaaagatca
    28321 acgaaactac ttatcaagta cgattagata aaggacagtc tttaacgata acaacaaacg
    28381 aaaaagatca aacagtcttg tcgatctcat ctggagacta cccacgatct gtcattgatt
    28441 tctttatcaa ttacgaattc ttggtcccat taatggtgag aaagtcctga ttggataagt
    28501 tggattacca gatgtcattc agataattct gttccaaagg acttgcggca gttatccaga
    28561 ataattgtca caagtctttt ctgtatgtac taaatggacg tgaataactt gctgatgaag
    28621 gagggatttc attaggaatg tccacaaata aaatagcaca gcttagctta ttgtctgctg
    28681 cttgtgtagc aggtaggatc gcttttcaat ttattccgaa tttccagcca atgactgcta
    28741 tttttttatt tatagtactt tacttaagct taaaagatgc gttaattgtc atgagtcttt
    28801 ctattgcgat cagcagtttt tattttggtg ttggaccatg ggtcatcggc caatggatca
    28861 gctatactgt tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca gtgcaaagga
    28921 atctatggtt aaagggagtc tgtttttttc tggcgggcat ggtttacgga ataggtatga
    28981 ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg gatctattac cttcaagggg
    29041 tgtcgtttga tttgatgcac agtataggca atgttgtgtt ctttcttgtt tttctgccag
    29101 tcgtcaaacg tttttacaat cattcaggag gaaaaaatga aaaaaataat ttatagtttg
    29161 cttgcaatga gtgtcagtat actcattttg acaagttgca gtacaaccta tacacaatcg
    29221 gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac gttacaagaa
    29281 aatggtcaaa acttttcgga aaaagaagtg gtcttcaaaa aaggcgatga tttactgagt
    29341 attttgaaac gaaattttga agtcaaagaa gataacggct tcatcacttc gcttgaaggg
    29401 cactcacagg atgagaaaaa tcaactctat tggatgttta ctgtggacgg gaaaagtgct
    29461 actactggtg ccaaagagat caaattgaat gatggacaat cggtcatttt caatttgtct
    29521 aaactataag tttcataaaa aactgttaaa gggcgaatat tgccataatt cagtgagttc
    29581 agttgatgaa agaaggaaaa taacatggaa aattttaaaa aaagcggaat gttttccgta
    29641 attgctgctc taggggctaa tattctagtt gctatcagca aatttgtcgg ctatgcaatc
    29701 agtggcagtg ccgccatgct aaacgaaagc atccacagta tcgttgattg cagcaatcag
    29761 attttgctgt tattcggtga taaacaagcc aataaaggac agagcgagtt gcatcaattt
    29821 ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg caatgatgct tttcttcggc
    29881 ggtggggcac ttggggtgat ggaagctttt gaaaagttga cgcatccatc ccatgaagtg
    29941 ggtaatccga tcattgttat agcaatactg ttatttggtt tggtaatcga aggaagttcg
    30001 ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg aaaaactttc cacgatgcgc
    30061 tttttgcgcg aaagtcgaca cagtgaaata ctgattattt ttacggaaga cttgtgtgct
    30121 gtaatcggtt tggtcttggc tttagcagga acaatattga cttatgcaac cgggattgct
    30181 gcatttgatg cgatcagcgg attgctgatc ggtttcttgc tgatgggagc cgccattttc
    30241 ctagcaaaag aattttatag tctgatcatc ggtgaaagcg tgacagcaag cgatttatca
    30301 aaaatcaagt tagcttttga acgtcctgag atcagtcggc tgatcgatat aaaaacagtt
    30361 catttaagtc caacggagat tttagttgca gcaaaagcag atatcgttgg taacaaagag
    30421 aatgaagctt actcggtcat caatgatatt gaaaaaaata tgcgtcagtc tcttcctgac
    30481 aagaagatgt atatatacat tgaaacagat aaatatgacc caaattattc tcgtaaataa
    30541 aacgtttatt catgaattga agcaaaaatg agaaaaatat aatcttgttt ttaaataaaa
    30601 cggctacaat ttagcgaatt tatgttacaa tgagtccgct gtttatttgt tgtaattaaa
    30661 ggagatattt taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt gcatgcagtg
    30721 atgccacttt gtataagcta tataccagta ggattagctt gcggagtatt gttgcaaaaa
    30781 gttggatttg atcctctctt atcaggactg ttatcgattc tggtcttttc cggtggcgcg
    30841 cagtttttag ttgcatctat gcttacgacg caagcatcat ttgcaacgac attattgatg
    30901 gttttctttt tagaattacg ctatacatta ttaggagcaa gtcttgccgg ttttatgaaa
    30961 aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac tgaatgatga aaactatgcg
    31021 gtgaactact taaagttttc aactgaccct tcctggaata aaagaaaggc tttatatgtc
    31081 aattggttct caatgactgc atgggccggt agcaatatga tcggtaattt tttcggctcg
    31141 gtgattcgag tagatgctga ccttgtccat tttgctttga cggccatgtt cattttcatg
    31201 tttatcatgc agatgaaaag cgcactactg atctttacag gtttattctc aggcgtatta
    31261 ggggtcgtat tcatggtatt attccaaaat actttcggat tgattgcagc aacagtcatt
    31321 gcttcatttg ccggatttat gctggagaag gcattcaaaa aagaaaaagc taagaaacta
    31381 agagaccgcg gtaaagatgt caatgagccg ctcttcaact tcccggaaca ggaggccaat
    31441 caccatgact aaactatatt tgctactttt agttgcttgt ctgttcatcg tggcatatat
    31501 cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa gtgccatcct ggttcagtga
    31561 ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta gcttttaaag atgttttcat
    31621 tactcatgaa catctggata ttgcttggaa tatcaaaata gctgcaatga ttctagtagc
    31681 aggcgtggct tacaaaacac gttcgatggc gttgtcagtt ttgacaggac ttgcgtctgt
    31741 ctttttgctg tctatgcttt aaactgtttt attttattaa aaacagagat tgcgacataa
    31801 gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt caattctgga tgcaagttta
    31861 ttcgtaggga taaaaaagat tagtgttggc ttatgatgca agctcttttt tattttagga
    31921 attagagagg gatttgtcct tctatgattt tttgctataa tagggaagat gtttatttta
    31981 tgaaagaata gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa cattttggct
    32041 aacctttgct aatcttctat gtaaagtttt aggggtagtc tatttgattc cctggctaag
    32101 tatgatgggg aacaatcaag atggtatgct tgctacgaca ttgtataatg ttggctatct
    32161 tccttatgga ttgttcctga tgcttggtac agtcggattt ccaaatgcga ttgcaaaaaa
    32221 agtagctgta gccacaaaag aaggagataa cgctgcttgt cggctgattt ttagaagtac
    32281 gatcaatatc atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt tatttgctcc
    32341 tttactagcc ggaatcagtc cgattgctaa cgttaataat gggatactag ctatcagaag
    32401 tttatgccca tctttgattg ctatcccaat tcttagtgca atgagaggat atttccaagg
    32461 aaaaaatgat ctgcggcctt atggaacttc tttgattatc gaacaggctg tgcgagtgat
    32521 cgttatcttg gctggtactt attacttgcg cgtattgact gacggaacca tattagaagc
    32581 tgtattgatc agtacagtcg cttctttctt cggtgggtta gctgcgatta tccatatgta
    32641 tatcgtcgga cgcagaaaag acttcttcga acttaaagat tttttgattt ccagccgtta
    32701 tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac taccgtttat
    32761 ttttgtcggc tcagtcatta ctatcgttca aatgatcgac caagtaacga tgaaaccact
    32821 gcttcacttt ttccgtccag agatcgcaga tcagcaactg gaattcttat tcagccgtgc
    32881 ttctgtcaat ccaaataaat tgacattgat cctgatctcc atggtaggaa cagtagctat
    32941 aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa tcgaaaaaac
    33001 agtaggagat agtttttcga ttgcgttatt gattcttctt ccttcattga ctggaatgtc
    33061 tttattagct ggaccactat acacattgtt ttttggctat gatcctgaaa gtgtcggata
    33121 tttccaaatg gctttgctag cttctttgtt tttctcttta ttcacgatcc tatctacgat
    33181 gcttcaatct ttgaatcacc atcgttttgc gatccgatta actgtagaag caattatcct
    33241 gaaagtcatc ttccaagtaa tcggattagg attagttggc ggatacggga tgagcttatc
    33301 aaatggctta gcattcggta ttgtctttgt tcgcggctat cactttatgt gcaaagaata
    33361 ccgaatagca cctttagcaa agatcagcaa ttttttctta aaaactttcc gctccaccct
    33421 gattatgctt gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat tgtcaatgga
    33481 atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg gcttagtatt
    33541 tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta aacatcatag
    33601 cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa cggcaactcg
    33661 tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta atcttactta
    33721 tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac acatgaaact
    33781 tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc atcccgactt
    33841 attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag gaatcgatat
    33901 cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta ctacttcaca
    33961 acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag ggtatgacac
    34021 gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat tatttgcgat
    34081 gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca ctagtatgcc
    34141 gatgggacac atggtagaag ctgccttaga cttaaatgat gaaggtcagt cccttgatga
    34201 aatacttgca catatcgatc gaatccgaga taatacctat gcttatctga ttgtagatga
    34261 tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga ttggcagctt
    34321 attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat tcgaaaaaat
    34381 ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa gaaataacga
    34441 aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag tagcgattgc
    34501 ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg gtcatttcgg
    34561 accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt cagcgcaata
    34621 attcctttta ttcaaaaaac ataccaaaaa tattggctct ttgtcaaatg atgttggtaa
    34681 agagaaatct t
//
LOCUS       DASHFF010000025        31380 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.025, whole genome shotgun sequence.
ACCESSION   DASHFF010000025 DASHFF010000000
VERSION     DASHFF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.025"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            3..371
                     /locus_tag="U2I75_001352"
     CDS             3..371
                     /locus_tag="U2I75_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEN1696499.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="U2I75_001353"
     CDS             551..1492
                     /locus_tag="U2I75_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN1696500.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="U2I75_001354"
     CDS             1561..2292
                     /locus_tag="U2I75_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN1696501.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="U2I75_001355"
     CDS             2436..4616
                     /locus_tag="U2I75_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN1696502.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="U2I75_001356"
     CDS             4636..5118
                     /locus_tag="U2I75_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN1696503.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="U2I75_001357"
     CDS             5134..5895
                     /locus_tag="U2I75_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1696504.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /locus_tag="U2I75_001358"
     CDS             6026..7360
                     /locus_tag="U2I75_001358"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN1696505.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="U2I75_001359"
     CDS             7667..8152
                     /locus_tag="U2I75_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN1696506.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="U2I75_001360"
     CDS             8145..8828
                     /locus_tag="U2I75_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN1696507.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="U2I75_001361"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="U2I75_001361"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN1696508.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="U2I75_001362"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="U2I75_001362"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN1696509.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /locus_tag="U2I75_001363"
     CDS             12444..13073
                     /locus_tag="U2I75_001363"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN1696510.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="U2I75_001364"
     CDS             13125..13457
                     /locus_tag="U2I75_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1696511.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="U2I75_001365"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="U2I75_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN1696512.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="U2I75_001366"
     CDS             14283..15656
                     /locus_tag="U2I75_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1696513.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="U2I75_001367"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="U2I75_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN1696514.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="U2I75_001368"
     CDS             16711..17151
                     /locus_tag="U2I75_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN1696515.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="U2I75_001369"
     CDS             17295..17996
                     /locus_tag="U2I75_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN1696516.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="U2I75_001370"
     CDS             17993..19114
                     /locus_tag="U2I75_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN1696517.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="U2I75_001371"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="U2I75_001371"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN1696518.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="U2I75_001372"
     CDS             20493..21401
                     /locus_tag="U2I75_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN1696519.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="U2I75_001373"
     CDS             21436..21708
                     /locus_tag="U2I75_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN1696520.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="U2I75_001374"
     CDS             21719..22765
                     /locus_tag="U2I75_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN1696521.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="U2I75_001375"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="U2I75_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN1696522.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="U2I75_001376"
     CDS             complement(25776..26462)
                     /locus_tag="U2I75_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696523.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="U2I75_001377"
     CDS             complement(26440..26934)
                     /locus_tag="U2I75_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1696524.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="U2I75_001378"
     CDS             complement(26954..28174)
                     /locus_tag="U2I75_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1696525.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="U2I75_001379"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="U2I75_001379"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN1696526.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30250..31377)
                     /locus_tag="U2I75_001380"
     CDS             complement(30250..31377)
                     /locus_tag="U2I75_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1696527.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10450 a         5608 c         6723 g         8599 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggtaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30181 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg
    30241 tggcaaaaat caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat
    30301 cttatctgtt acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg
    30361 ttcagagatt tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct
    30421 gagagaatgg aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt
    30481 cactacactt ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat
    30541 gtagagcttt ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat
    30601 tgctagcaag gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc
    30661 atatacccat gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga
    30721 aaaatcgaca tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa
    30781 tccaatattc aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc
    30841 aaagtcctca agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt
    30901 tggtattttt acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat
    30961 attcagcgca ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt
    31021 tttaaattca gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt
    31081 ctttcctaaa taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt
    31141 gtattttaga tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg
    31201 agtatccgta cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa
    31261 agcagcttcg gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc
    31321 atcatcattg atatcttcgc cgtttgtata attaagcgag acaaataaaa aagccattta
//
LOCUS       DASHFF010000026        31117 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.026, whole genome shotgun sequence.
ACCESSION   DASHFF010000026 DASHFF010000000
VERSION     DASHFF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.026"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(334..696)
                     /locus_tag="U2I75_001381"
     CDS             complement(334..696)
                     /locus_tag="U2I75_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696528.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="U2I75_001382"
     CDS             complement(770..1150)
                     /locus_tag="U2I75_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN1696529.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="U2I75_001383"
     CDS             1214..2407
                     /locus_tag="U2I75_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1696530.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="U2I75_001384"
     CDS             complement(2484..3068)
                     /locus_tag="U2I75_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1696531.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="U2I75_001385"
     CDS             3269..4267
                     /locus_tag="U2I75_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1696532.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="U2I75_001386"
     CDS             4379..4888
                     /locus_tag="U2I75_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN1696533.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4918..5577)
                     /locus_tag="U2I75_001387"
     CDS             complement(4918..5577)
                     /locus_tag="U2I75_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN1696534.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5579..6343)
                     /locus_tag="U2I75_001388"
     CDS             complement(5579..6343)
                     /locus_tag="U2I75_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN1696535.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6351..7028)
                     /locus_tag="U2I75_001389"
     CDS             complement(6351..7028)
                     /locus_tag="U2I75_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN1696536.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7065..8450)
                     /locus_tag="U2I75_001390"
     CDS             complement(7065..8450)
                     /locus_tag="U2I75_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1696537.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8660..8744)
                     /gene="ffs"
                     /locus_tag="U2I75_001391"
     ncRNA           complement(8660..8744)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U2I75_001391"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="U2I75_001392"
     CDS             complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="U2I75_001392"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN1696538.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9389..10246
                     /locus_tag="U2I75_001393"
     CDS             9389..10246
                     /locus_tag="U2I75_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1696539.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10327..10662)
                     /locus_tag="U2I75_001394"
     CDS             complement(10327..10662)
                     /locus_tag="U2I75_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696540.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10729..12099)
                     /locus_tag="U2I75_001395"
     CDS             complement(10729..12099)
                     /locus_tag="U2I75_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN1696541.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12177..12713)
                     /locus_tag="U2I75_001396"
     CDS             complement(12177..12713)
                     /locus_tag="U2I75_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1696542.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13022..14503
                     /locus_tag="U2I75_001397"
     CDS             13022..14503
                     /locus_tag="U2I75_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1696543.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14606..15313)
                     /locus_tag="U2I75_001398"
     CDS             complement(14606..15313)
                     /locus_tag="U2I75_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN1696544.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15392..15730)
                     /locus_tag="U2I75_001399"
     CDS             complement(15392..15730)
                     /locus_tag="U2I75_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN1696545.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15841..16803)
                     /locus_tag="U2I75_001400"
     CDS             complement(15841..16803)
                     /locus_tag="U2I75_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN1696546.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16818..18149)
                     /locus_tag="U2I75_001401"
     CDS             complement(16818..18149)
                     /locus_tag="U2I75_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1696547.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18157..18837)
                     /locus_tag="U2I75_001402"
     CDS             complement(18157..18837)
                     /locus_tag="U2I75_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN1696548.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19056..19535)
                     /locus_tag="U2I75_001403"
     CDS             complement(19056..19535)
                     /locus_tag="U2I75_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN1696549.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19662..21341)
                     /locus_tag="U2I75_001404"
     CDS             complement(19662..21341)
                     /locus_tag="U2I75_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1696550.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21889..23391
                     /locus_tag="U2I75_001405"
     CDS             21889..23391
                     /locus_tag="U2I75_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696551.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23404..24066
                     /locus_tag="U2I75_001406"
     CDS             23404..24066
                     /locus_tag="U2I75_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1696552.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24167..24370)
                     /locus_tag="U2I75_001407"
     CDS             complement(24167..24370)
                     /locus_tag="U2I75_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1696553.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24481..25665)
                     /gene="iadA"
                     /locus_tag="U2I75_001408"
     CDS             complement(24481..25665)
                     /gene="iadA"
                     /locus_tag="U2I75_001408"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN1696554.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25822..27561)
                     /gene="yfcC"
                     /locus_tag="U2I75_001409"
     CDS             complement(25822..27561)
                     /gene="yfcC"
                     /locus_tag="U2I75_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN1696555.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28334..28948)
                     /locus_tag="U2I75_001410"
     CDS             complement(28334..28948)
                     /locus_tag="U2I75_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1696556.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29554..29931)
                     /locus_tag="U2I75_001411"
     CDS             complement(29554..29931)
                     /locus_tag="U2I75_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696557.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(30231..31112)
                     /gene="galU"
                     /locus_tag="U2I75_001412"
     CDS             complement(30231..31112)
                     /gene="galU"
                     /locus_tag="U2I75_001412"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1696558.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         9285 a         6465 c         5191 g        10176 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc
     4981 aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg
     5041 aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa
     5101 cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac
     5161 aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt
     5221 ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc
     5281 cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa
     5341 atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa
     5401 atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat
     5461 atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt
     5521 cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt
     5581 agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta
     5641 catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc
     5701 cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc
     5761 ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg
     5821 gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc
     5881 gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag
     5941 caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct accactacat
     6001 agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg
     6061 cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg
     6121 ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac
     6181 gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt
     6241 ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat
     6301 aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc
     6361 ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt
     6421 tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg
     6481 cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc
     6541 ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac
     6601 gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct
     6661 ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc
     6721 caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt
     6781 tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa
     6841 aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc
     6901 tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct
     6961 gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt
     7021 gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag
     7081 tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt
     7141 tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta
     7201 aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata
     7261 gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt
     7321 cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg
     7381 atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg
     7441 tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt
     7501 aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac
     7561 gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct
     7621 tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa
     7681 gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt tttctcttca
     7741 tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg
     7801 acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca
     7861 tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc
     7921 aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa
     7981 ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca
     8041 attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc
     8101 ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga
     8161 tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc
     8221 gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct
     8281 gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt
     8341 tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt
     8401 tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc
     8461 gcctctcttt atctttatca taccataggc agctttttct tccattgaaa caagctctat
     8521 ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct
     8581 ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg
     8641 aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc
     8701 cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag
     8761 tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag
     8821 ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac
     8881 atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc
     8941 gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc
     9001 gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct
     9061 tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg
     9121 cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc
     9181 tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat
     9241 ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt
     9301 gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat
     9361 ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc
     9421 acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga
     9481 tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa
     9541 agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcact atctttttaa
     9601 agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa
     9661 aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc
     9721 tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta
     9781 tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac
     9841 aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc
     9901 tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt
     9961 ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt
    10021 gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt
    10081 atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac
    10141 tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa
    10201 cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg
    10261 ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag
    10321 tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga
    10381 atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa
    10441 cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt
    10501 ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg
    10561 atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc
    10621 tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt
    10681 atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct
    10741 caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg
    10801 tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc
    10861 tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt
    10921 ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa
    10981 atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc
    11041 ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga
    11101 aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt
    11161 cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc
    11221 ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc
    11281 ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg
    11341 ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt
    11401 cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg
    11461 ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc
    11521 ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc
    11581 cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc
    11641 ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac
    11701 taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc
    11761 acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc
    11821 acttggttct tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt
    11881 gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg
    11941 gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc
    12001 ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt
    12061 gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt
    12121 ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa
    12181 ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa
    12241 acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat
    12301 gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca
    12361 tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt
    12421 tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca
    12481 tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc
    12541 ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg
    12601 cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc
    12661 atacttttga acagcaaata ttctttacta taatttgtta ctctccccat cattcttacc
    12721 tccgtctctt ttcatctacc cataaataaa taacataaat aattctaaaa atattattga
    12781 taattatcaa aacatattta gttatatcca ttaaaaatca atttttccgc cttaaaaaca
    12841 agaatatctt gacaaaatac cctaggaaat aaacaataga ataaacaagg taacaaaaaa
    12901 attaaataac gttatataaa aaagaaggcg acactctgtg agaataacac agactgaaat
    12961 gactttccac tcacagtttt tgtctaacta tatacaaaaa cagaataaaa aggaggtatt
    13021 tatgctaatt gaagcaagaa aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa
    13081 taattgttct ttacaagaat tatccaatga cttacatatt ccaaaacgga caatcaaaga
    13141 actgatccga aaaataaatg tatcactcaa gcaattttta ttgattgatg aatttattta
    13201 ttctaatcat aaaggagaga tccaaaccaa agaaaccgat caaactaaaa agttagctgt
    13261 tttttctaag ttaaaattat gctacctaaa agaatccaat cgttttaact atttgatgtt
    13321 actaatcaat tatccacaaa ctgctgtacc aaaaaaatat ttacttgaac aactttatat
    13381 atcgccctcc tatctcgaaa aactgacaaa acagctcaat cattccttaa aaaaattcca
    13441 tttacggatc ctatctgtaa aaaattgctg tactcttgaa ggaaatgagt tatctatccg
    13501 tctctatctt tacttttttc tttcagatac atttggaggt aacgattggc cattttcctc
    13561 acttgaatta aatgcactaa aaatgaacag tacagatgag caaatagaaa aaatttcaaa
    13621 acaagaaaaa aatatctact tgctgttaac tttattcata ttacgaaaca aacaacaggc
    13681 atattttcca aaaacttctt ctgaaatcac tgaattgatg gacttattgc agcatacaca
    13741 tgattattca gaatacttta cttctttttc agtgagtcgc ccttgtgaag agacagaaac
    13801 agagagagcc tactttaatt attttttcta ttgttttttt ccaaatcaaa tctcttttcg
    13861 acaaaaacag gagattggtt ttttattttc cacaagcaaa aaccttattt gtcgacaagt
    13921 cggtcaattg atgacttact taactcaaca gtttcctctc aaccatttag aaaataaaat
    13981 accaatttat ttttattttc ttgtgttggc agttgttttt ttcgaactgt tgggtgaatc
    14041 cagcagtagt ttttctacac tttgctttcc agaagccacg tccaatcatt ctagttcttt
    14101 tatacaggaa aagaaattaa gagaaaaaat cgatacaatt ttttcaaaaa atggggaaag
    14161 aaaccaaatc agtcaatacc ttagtcaatt gatccaacac ttactacaaa cagaaacgaa
    14221 agagcagtta ctcatccatt tgcagataac caaacagttt acaggaaatt atcatcttca
    14281 aagccatttg cagcagttat ttcgcaaaga tgctgttaaa attaccgcat attcagaaca
    14341 tgctgatttg atcattacag atcagcatga gaaaatttca gatgaaacgg agactttcta
    14401 tctggataca acgcacaata cagtgttatt tggcaagtta ataaaagcgg ttcatgaact
    14461 gtatatgaaa aaaggttatg agaacaaaac aaagaggatg tgacaaaatt cttgtcacat
    14521 cctcttattc caaataaatg gtattcaatc agctaactct cggtattagc tcagatcaaa
    14581 gcgctgattt cacaacttct ttattttaat ctcttcggtt gccgccgaac aataagatca
    14641 agcgcaacag ctgcaggaat gtcgaaagcg ctgctgcaac ataagtcagt gctgctgcaa
    14701 ataatacgtg tcttgccatc ggtacttctt cttccgtcag tagtccgcct tcactcaaaa
    14761 tttggattgc tcgccgagac gcgttaaatt cgactggtaa tgtcaccaat tggaaaagta
    14821 atgctaaaga aaaagcgaaa atcccgatat tgattaacgt agagttccag ctaagcaata
    14881 cacctaccat aatcaagggg aacgaaatca tcgaaccaaa gttagctgct ggaacaagtg
    14941 ctgcacgaat cttcaatggg acataacccg aagcatcttg taccgcatgt ccgcattcat
    15001 gagctgctac acctatcgca gcaactgaag tagactgtgc agttgcttga gacaaactca
    15061 acatcttatt acctgaattg tagttatctg tgagatctcc ggcaatttgt tgaacaccga
    15121 cattatttat ctgttgactt tgtaatatat attgtgctgc ttgtgtacca gtcacattgt
    15181 ttttgcttct cacttgatcg tattttcgga aagtactatt gacgtacgct gacgcaattc
    15241 cggaaataac taatccaaga atgaccaaaa gaaatgtcgg atctaaaaaa ccatacattc
    15301 cataaccata cattttcgtt tcactccttt tcgtttactt tattttatca ttagtagtag
    15361 gaaaagaatg tgaaaagacc ttgaatattc tttaattctt tttcacaaat tccgatttca
    15421 gtttcatcgg tccaaatcct tctaccttgc aatcgatatt atgatcgcct tctactaaac
    15481 ggatgttctt gacttttgtt ccttgtttaa tggcacctga cgcacctttt actttcaagt
    15541 ctttaatcac tgtcacgcta tcgccctcta acagacggtt tccatttgca tctaatacca
    15601 ctaatccgtc ttctatgaca gtttcttgtt cttctttgtt ccattcgttc ccgcattctg
    15661 ggcaaatcaa caagcctcga tcttcatatg tgtactctga gccacattct gggcaatttg
    15721 gtaatgtcat cttctttctc ctttaaaatc tttagtgagc actattgtaa cggaaaaaga
    15781 agatcttgaa aagatacatt tgtgattttt actacaaaag gcacgttttc gaacgaagta
    15841 tcattctgtt tttctgctct tatacgattg gcatatatct tggatctctt tatagatttc
    15901 ctctacttct gctaaatctt ttgcattcgc accacttgct aatggatggc ctcctccatg
    15961 atggcgttta gccaattcat tgatgatcgg ccccttagaa cgtaaacgta cgcggtaata
    16021 ccctgaaggc tgctgaacaa aaatccccca agccagcact tcgtcaatca ctccaggaag
    16081 tgaaacgatc gaagctgtct ctgaatcatc gattccatat ttatcaagaa tttcttgcgg
    16141 aaggattatt cttcctgcac cattttcatc cacctggata ttttgataaa gataaccgga
    16201 aagtttagcg actttcatcg gcatttgttc aatctctctg ttcaaagcac ttgcatcaaa
    16261 tggataattt ctcaatatcg aagcaatctc taatgtacgg gacgtcgtgg agggatacaa
    16321 aaagcgccct gtatctccga cgattcctgc ataaagtaac cgtgcagcct taacgttcat
    16381 ctttaaagat tcaggaaaca tcaaagcaaa ttctgcaatc atctcactac agctacttgc
    16441 ttttgtattt acccacacaa gatctccata agggtcatca ttgggatgat ggtcgatttt
    16501 gatcagtttt tctgccaact gatatctttg atcactgatt ctgggtgaat tagctgtatc
    16561 agtcacgatg actagcgctc cttggtagaa gacatcttca acttcttgca tttctgctaa
    16621 aaagcgaagc ccttcaatcg tttcacctac ttgacgtatt tctttttctg ggaaactttc
    16681 ccgtaatagt tcggctaatc ctacttgtga tcctaatgca tcaggatcag ggcgcaaatg
    16741 cctgtggata atgattttat cgtatttttt tattgtatct actatttctt gctgtactgt
    16801 catttcactc ctgctttcta agaacgttcc attacttgac aaacaacgat tgctttggaa
    16861 acgatggtgt tttcaataaa cacttcgata tccagtttag cggaacgtct gccgatttcc
    16921 aatacacgtg gacgaatgtc taattcgctt tccaattgga tcagccgcaa ataatgtagg
    16981 ttcacttgtt caatcaagat atttcgtttt tggttcatca ccattgtttt ttgtgcgaca
    17041 tcagaaatga tttcgctaag tacgccaaaa gaaatggttc caacgccatt gaccatttgt
    17101 ggtgctacta cgaatttgaa cttagggttt tccaaacgat ttccttcacg gtcagtatca
    17161 atcgtatgga tatttccaga tatctgatcc gaaatcgtat ctgcaatctg cggttgtcgc
    17221 tggacaagct gcatcgcttt cattacatct tgccgggtaa tcaagccttc caatgtcaag
    17281 tcatcggaaa cgaccggcat gacttctagc ccatcccaga tcatttgatg gctaacagag
    17341 gcaatactca ttgttttctt tacactgatt ggttctttcg tcatcacccg atcgatgatc
    17401 tggtttggcg atttccctaa aacatctttt gccgtaacaa tccccatcaa acgatgatgg
    17461 cggttgacga ctggaaaacg cgaatgctgc gtttcttctg acaaacgctg ataatcagaa
    17521 atcgtatctg tctgctgcaa ataatgagtc ttttcagctg gcatatagat atcactgaca
    17581 agcatgatat cttttttgat caattggtcg cttaacgctc ggttgatcat ggttgcgact
    17641 gtaaatgtat cataagaggt gcgtaagatc ggcatttcaa gatcatcagc tagctttgca
    17701 atctcagcag tcgtattgaa tcctcctgtg atcaatactg cggcgccatc ttccaatgct
    17761 aatttttgga cttccgcccg gttgccgacg atcattagtg atcctggcgt aatatagcgt
    17821 tccatggcat gtttagtcat cgcgccgatg acgaatttat tcaagacctt gtctaatcct
    17881 gccgccccgc ctaaaacgtc cccttcaatg atcctgacga cctcgccaaa tgtcaaacgt
    17941 tcaatatgtt tttttaattt gcgttcgata cggatggtcc ctactcgttg aattgttgaa
    18001 actagtccga cattttctgc atctttgatt gcacggtacg ctgttccttc actgacattc
    18061 aattcttttg cgatgcttct tacagaaatt cgattgccaa caggtaaact ttcaatatat
    18121 tctaaaatca aatcatgttt tgtcgccatt ttctgcctac acttccagcg tttccgagac
    18181 gctcattatt tttccttcac tatcttccag taactctaca aaagattcgg gatcttgttc
    18241 aatgacaggg aaagtattgt aatggattgg aactactttc ttggcacgaa ttgtttgcgc
    18301 agcctttgct gcatcttctg gccccatcgt atagttatcg ccaatcggca agaaagcgac
    18361 atcaatatcg aattcttctg caagcaatcc tagatcactg aaataagcag tatcccctgc
    18421 atgataaatc gtttttcctt ctgcttgaat aataattccg ctaggctcgc ccatatactg
    18481 aacgataccg tctacttcat agcttgaact atgctgtgca tgtacgaatt ttaccacgcc
    18541 gaatggaaac gcatgttttc caccgatatt cataccgaat gaatctactc ctctgctgtt
    18601 cgcataatca gcaatctcaa caatactgat aattgtggct tgattttctt ttgcaatagg
    18661 aatcatatct cctatgtggt cactatgacc atgagtaatc agcaaccaat ctgtctttac
    18721 ttcttctgca gttaagtctg tcaatggatt tcctgtaata aacgggtcaa tcatcacgcg
    18781 ttggccattt tccatttcaa ttgttaaaca ggaatgtcca tgatacgtaa ttttcatatt
    18841 ttacacctcg cgttttctct gtatcttttt tattaaaaac gatttcatca cttgcacatc
    18901 aatgaaaaaa tatagctgct aaatatattt tcattggatt gatagtacag tatgtacaaa
    18961 gcaattgtaa cagataatac aggatgcacc aagtaccaag cagatataat ctcaagtttg
    19021 aggtctctgc tctgcttagc ttctcttctc gtcccttaac taattaaatc agcgtaaata
    19081 ggatggacga tcttttctaa atcggctact tttatccgaa ggcttctccc gatttcaccg
    19141 cttgacacaa ttacttcttc tttttctttt aaagaactgt ctgctaaaaa acaataatca
    19201 ggatgtgcga gttttattcc aataggggtg tttgctccgt ggggatatcc tgtatgcatt
    19261 aatacaaaat ccattccagg aaaaccaact ttacgatctt tcgtcacttg tctcgttttt
    19321 ttgtaatcta agtgatcgtt caaaggaatc aatgcgatga tcaccccagt tttgtttcct
    19381 ttcactacga gagttttaca tatcaaagct ggatcaatcc ctcttttttg tagttcttct
    19441 gtgaaagatt cttctaccac atgactaaaa tcaagtggtt gatagctgat cttttgttct
    19501 tctaaataac gttcgacttt tgtttgactc atcattgatt cactcccttt attctatcgt
    19561 ttattataaa gcgagggatg ctgtattcaa aaattatttg tttttcgcaa aaaaagagca
    19621 agacgagcac tctatagaga ggttcatctt actctttttg tttatttttt atttcgacgt
    19681 tgagtagctt ccataatgac tggcaagatc actgggcggc gttttgtttg ttcaaataag
    19741 taacggccta attgatctcg gatgtcttgc tttaatttgc tccactcaaa atcattgctt
    19801 tctaaatgtt tctcaacgat tgtttcaatc atattgctgc tttctttcat cagatcacgg
    19861 ctagctttta cgtaaacgaa tccccgtgaa gtaatttgag gttttgaaat gattttcttt
    19921 tctcgccgac tgatagtgac aaccgctacg aaaatcccgt cttctgacaa gatttttcga
    19981 tcacgtaaaa caatgtttcc gatatcacct atgcctaatc catcaatcat tacattttct
    20041 gcacttgtag ttcctgcaac actcatacgc ttgttcttat attccagcac gtctccgcgt
    20101 ccagtaatat agatattttt gtatggcatc cctactgcat gagccaaatc tgcatgagca
    20161 gctaattcac gatactctcc ttgaactggc acaaaatatg ttggtttcat caaattgatc
    20221 attaattgca agtcattagg attggcatgg ccagaaacgc gcatattctc agagatcaac
    20281 ttcactgttc caccagctcg ataaatgata tcttccgttt tcgccacgat ggtttccatc
    20341 gcgatgcttg gcgtagtagt gatataaacc aaatctcctt cttcaatctt gacattacgg
    20401 tgtgtaccgt ttgccatttt ttgcagagac ttgatgggtt cacccattcg gccagtttct
    20461 aaaatgatca gttcttctgg atcatagttt ttcatgtctt tcaacgtaac aagcagatct
    20521 tcacttggta attgaagttt ttctaaacgc atcgctgtgc gaataattcg ttcgaaatct
    20581 tgaccagtca agacaacttt tcgttgtgct ttttctgctg catccaatac ttgttgcact
    20641 cgttgcaaat tacttgctac acaagcgacg atgatacgcc cttcccaata tttgatcgta
    20701 tcaaatactt catctgctat ttgggcttca gaagctacag gctgaggatt ttctgcattt
    20761 gccgattcac ttagtaaggc taagactcct tctttgccga tttccgccaa tcgtgcataa
    20821 tctgtttgat acattgggat agccgtttgg tcaaatttaa aatctcccgt ataaacgata
    20881 ctaccttctt ttgttttcag attgatcccc actgaatctg gtatcgtatg agtggtacgg
    20941 aaaaagctca ctactgtttc accaaagtct atttctgtat gctcatctac cacatggaaa
    21001 tccttaaatc ctttggctgc ttcattacga ctgacattca attttgctaa ctcgatcgtc
    21061 aactctgtcc cgaatactgg tacagaaatt tttgacaaca agtaaggaag tgccccgatc
    21121 gcatctgcat gtccatgtgt taaaaagaca ccagctacac gatcaatgtt ttcttctaaa
    21181 tacgtaaaat cagggattac gacgtcgatc cccaacaact cattttcagg atattttagc
    21241 ccacaatcta aaataaaaat atcctcgccc acttctgcga catacatgtt ttttccattt
    21301 tcacgaacgc cgcctaatgg gataatttta atggtactca caaacttcac ctcttcttat
    21361 gacatccaag tttaaagatg tcgattttct tctttatcta aatatatgag ccattccaat
    21421 gaagcatatg caaaatcact atttctcaac ttatggagaa tcagcaggaa aattaccagc
    21481 acatccgcgt tctgacagtt ggatgggcat tttattttgg atatacttca ttaacaaaat
    21541 ggatcttttg cgaaacgcta tagccagaat acttatataa gtattcagct gttccccatt
    21601 attatacaac aaataaacta aatcgctagt atcagcaatt gttagaacaa ctatgcacgt
    21661 aaaaatccct ctgtagcatc taggatttct tttgctattc ctctttcatt tcttcttcgt
    21721 cggtttgaaa catacttttt tcattttacc acattttttg caataactat agtgactttt
    21781 tatatataca gaacctcatg ttgatggtgt gcaaacagca taagatttaa gctagcttta
    21841 agataggttg tctattatca aatgcatcta ctaggaggga tgcaaaatat gaattttatg
    21901 aaacgtgcat ggaaaagtac aaaagtaaaa tggggacgtt cattgttatt gtttgccgtg
    21961 ttcacagcca ttcttatttt tgtgctagct ggcctcacga ttcgaagcgc tgcagaagaa
    22021 gcagcgaatc aagcacaaaa agaagttgga gcaacagtga ctttaagtgc caatcgagaa
    22081 gcttcattta aaaaacagga acaaacttct tcaagcggtt ctaccactga agaacgtcca
    22141 gatccaggca gtttttctct tacaccagtt tccttaacgg acgcggaaaa aatagctgaa
    22201 ttagataatg taaagagtta ttcctttgaa agttccgcct cagctatggc aaaagatgga
    22261 ataactgcca tcactagttc tgattcaagt gaagacagtt cagaaactac tgatgcaggc
    22321 ggtgaacctg ctggaggaat gggcgggaat ccgcaaatga ctcaagcaga ctttcaaatc
    22381 agtggtgtgt cagcaactgc acaaaatagt aatttttctg atggaacagc aaaaatcacg
    22441 gatggtgaag gaatagatga cagcgacaaa ggcacaaaca atgtagtcat tgattctact
    22501 ctagcagaaa caaatgacct ggcagtcgga gatactttca caatcacgag tactgaagat
    22561 gaagatacca cttatgaaat gacgatcaaa ggaatttatg aatccagcga aacaagcggc
    22621 agtatgggta tgaaattcaa cttcatgaat ccgtcgaata cactttacac ttcttacact
    22681 tttgcaaatg aactaaatgg gacatctgac gataacacga tagattctgc tatttacact
    22741 ctatctgatc cagataaaat ggatgagttt ctcaaagaag ctgagaagct gatcgacaca
    22801 gatacattta gcttacaatc aaatgattcc atgtatcagt caatgttgga acctttaaac
    22861 aacgtggcaa gtttttctaa aaatgttgtt ttactagtgg ctgtagccgg tattatcata
    22921 cttactctga tcattatgat taccattcgg gagcgtcggc atgagttagg cgtcttgcta
    22981 tctttaggag aatccagaag taaaatcatt ctccaattgt ttaccgaagt ggctatttgt
    23041 atgattcttg cattaggagt tgcttcgctt agcggaaacg tcgtcgcgaa tgccgtggga
    23101 cagcagctac ttgaccaaca gacagaaacc acgacacaaa accagcaagc tcctggcggt
    23161 ggacaaatgc caggtaacag acaagaacaa ggcggtcaac gcgatggtca acaaggaacc
    23221 acgaataatc catttgctgt ttctgatcaa gtcagcgaat tggaaatcaa tgtcaaacct
    23281 gcacaattgg gactattagc cggagtagca tttggaatca gtctgttttc tgtcttttta
    23341 gcttctatag ggatattaag attaaaccca agaaaaatcc tattaaacta aaggaggaat
    23401 ttgatgttac ggacagaaca attgggattt tggtatcaag atccgcaaga tgcgctttat
    23461 aaagatgtag aactgaattt cgaagctgga aaaatgtacg ctattttagg agcaagtggt
    23521 tctggaaaaa cgacttttct ttcgcttatt tctggactag atgcaccaaa agaaggaaag
    23581 gtatactatc aagatcgttc actgaacaaa atcgggttaa gaagttatcg taaaaatgat
    23641 gtctcgatta ttttccaagc ttacaacttg cttccttatt tatcggctct agacaacgtc
    23701 atagcagcaa tggaaatcac taagaccaag caagagaatt atcgacaagt tgctttagat
    23761 cgactggctc gtgtaggaat tgatgaaata ttagcaaaaa aacaggtgac caaactttcg
    23821 ggaggtcagc agcagcgagt cgctatcgtt cgtgctatct gctgtgaaca tcaactaatc
    23881 gtagcggatg agcctactgg aaatctagat gaaacaactt cacaagatat cgtcaaactt
    23941 ttccaagaaa tcgcccatga acaaaatcgc tgtattatca tcgtgactca tgagattgaa
    24001 gtagcaaaaa gctgtgatga agtctttgaa ctaaaaaata aaaaatttca aaaaatcgaa
    24061 gtttaaaaaa caggataatg atcaaataaa aaatccgaac catatctgtc tcgcagatat
    24121 ggttcggatt ttttatttgc ttgattcaat tagtaataag tggtttttat tttttttcgt
    24181 cttttcgaga aaacttgtct ttgacttcgt cgcctaaatt tcgagctttt tctttgatgt
    24241 cagcacctag tgattgtgct ttcccttctg tttctttgct cttgtcgtct gttgctttgc
    24301 cgtatgcttc ttttccttca ccgactactt tatctttcat atctttagaa cgtcctttta
    24361 aatctgccat tgtgcttcct cctcttagtt tgtttattga tctaccttca gggtaacatc
    24421 aacaacagat acagacaaac agaacgctcg ttttctaaaa aagatagtta gagctgttga
    24481 ttaaaaactt ttttcgaaag tcccccatac tttgacttct ttttcttgga ccattatttc
    24541 tcctttagcg ataactgtat caatatcaag cgtttcttta tccaagatac acagatctgc
    24601 atcagcacca atttcgattt ttcctttttt atccaatttc aatatacgtg ctggattact
    24661 ggttatagct cgtaaggcga tttccaaagg gatactttct ttttgcacag cttctttgat
    24721 ccctactagc aatgccttgg cacttcctac tcctaaccct aggaaatgat tgttttcatc
    24781 aaaataaggc aagcttcctt gtccatctga ggtcatcgtg aaattatcta aagaaacatc
    24841 ttcctcgatc aatcgtttca gtgctttgct gaagcgaact tcaccatctg tcttctccca
    24901 aaaatcagga tcttcgctag cagtgaagtc aatcgtgcca ccacgtttgg caaatgccac
    24961 gcaagcttca aataattctg gtgtgcgatt tgcatgagta ggcaaaaatt gtgtgatcgg
    25021 aatttctgtt tcttctacga ttcgtgtcaa tagctctagc tttctttttc ctccgcctaa
    25081 gtggacattg atgatcccag cttttccgga taacatgcct cctacacgag catctgctac
    25141 tgcacgagcg aactcttcaa aacttggctg agaagaacgg tgatcggata ctgcgatttc
    25201 tccgattccg ataattttat caattgtcaa aaagtctttg atgatagaat ctgttactgt
    25261 ttttaccggc aatcggtacg aaccttcgta tacataagtg gagattcctt ctgcttccag
    25321 acctttcgct ttgctgatca acgcagtcat atctctacct tctccgtctg ttcccaaaca
    25381 accgacaact gtcgttacac cagctgtagt caattggctc aatgtgactt ctggcgttcg
    25441 attttgataa ccgccttctc ctcctccacc taaaatatgg aaatgacagt caatgaaacc
    25501 cggggttagt acttttccct cacctgaaag ttcttctact tccactcctt cgtagccgcc
    25561 ttctagctgt tcttctacag ccaaaatctt cccaccggca atcagtacat ccatttttcc
    25621 taagtaatct ggtgcaaaca cttcaatttg tcgaatgatt ttcatacatt tttcctcttt
    25681 ctttaagtat aactgatgat aggatcttaa aaaataatcc aaacgatagg atctttactc
    25741 ctttgatttt accgtaaaat gacggttctt ggagtcctta tctactgttt ggattacttt
    25801 tttatttaca ggtctccagt actactgcag acctattgca atggcaatca tgatgaaaat
    25861 agttcctaaa acaaacagga atccttgcat cttgatttgg aatcttgccc attttgccca
    25921 ttcgatccgt gcaacaccta gcacaccaat caagctggct gatacgggtg taaaagcgtc
    25981 cataaaacct gaaccaagct gataagcaag aactgctact tgtctggata cacctactaa
    26041 atctgccagt ggagccatga ttggcatagt caaagctgct tgtcctgaat ttgatgtaac
    26101 aaccaagttg aacaaacttt ggaagatgta catcgcccaa gcaccaatga cagctggaac
    26161 accggtcaac gccgtcccga tcccatgcaa aatcgtattc aaagctgaag gaacagatgc
    26221 atcagatcct cccaagacaa gaagaatccc tttggccata cctactacga ttgctgtacc
    26281 agctaaatca gctgcaccag attggaaaga agaagcgatg tcattgactt tcattccatc
    26341 taatttgaaa agaacagcaa tgattccagc aactaatccc atcacaaaga attgagaagc
    26401 gatttctgga atgtaatagc ctttttgagt cacgccccaa acgatccaga caaggacaac
    26461 taacatctct aataagatca atttatgccc aagcatgaat tttttatttt catcagatgt
    26521 ctttttgata tgagaacgga aataatcatc tgatttgtaa gtcaacgaac tttctggttt
    26581 tttacggatt ttttctgcat atatcatcat ataaccagcg gctaatgcag taacaactac
    26641 ccacataatc aatcggaatg tcgcaccaga caacacgggg attccagcaa tcccttgagc
    26701 caccgctacg ctaaacgggc tcatccaaga ggtcgcattt ccgacttgag atgcgacata
    26761 agtcacggtt actgctacga tcgagtcata tcctaacgca atgacaaatg gaaccatcac
    26821 cattgagaat ggaattacct cttctgccat cccgaatgta gcgccaccga atgaaaaagc
    26881 aaagaataat agcggtaaag ctaaacgctc cagtccctta gttttactga taaacgcata
    26941 gatacctgcg tcgatcgctc cggtacgcat aataatacca aaagcacctc caacaacaag
    27001 gatcaatgca gcgatcccta cagcagatcc atacttatct ccagatacta gtccttcaaa
    27061 aacaaaattt aagaaaccaa agccgccaaa atcatctgtt ccccatattt tggctgtttt
    27121 atgcaatttt ttagaagtat catagatatt ttcgccgtac tgatcgtaaa gcacatcatc
    27181 tgtcaaggag atttcatcta gtttttcctg cgtaagattt ttttctccgt cagcaatgac
    27241 tttttccaat cctttttccg gaacatccag tttttcacgt tctgctggat gatcttgcag
    27301 ttcttctaat tgatcaaaca catacgactt atccagctcg taagcataac ggaaggaatc
    27361 ttgacgtaaa acagtacgag tacttgtttc gccatttgca tctttgtatt caatatcttg
    27421 cgtgctgaac tttcctgctg gaacgacaaa agtcagtatc catgcaaaga tgactacacc
    27481 aaaaataatg acatacgtat ggggtgtcga taatttagtg aagtcgcgtt tcggttttcc
    27541 tgaagaattt gttttcttca aatttatttc ttcctttctt ttttcagtaa ttttgatgaa
    27601 taaaaaatgg ataatcaaat gaattataca gtaaaaatga catttccaca tgaataattt
    27661 attagaaaat cacttttttc tattttgttt tttttctcct atgtatgatt atttttttct
    27721 ctttccattt atattaagta ttttatttct ctgtttttcc ttaaaattct ttttttattt
    27781 ttagattaga gaaaatctcg tcctgttttt ttcattttac aatcattgaa aaattgaatt
    27841 tcattgcaaa aaaaataata aacacttata aatatccatt atctatcctt caatttttgt
    27901 gtattttatt tctattagaa aaaggtatcc tgttggacaa ttttgttata tcaggtaaac
    27961 ttcattgcaa taatgataac aaatgtacta taattacttt gtaaagagtt ttggggaaga
    28021 ctctttcatc aacgtgtatt aagagacaag aaagaatcca tatggattct ttcttgtctc
    28081 tttttccact actttttatt tttctgacac tacatattct tataactcat ttctcataac
    28141 tgtgaaacaa cttataaaaa taacaaactt taaacaaaac aagcttaaaa tcaaagatga
    28201 tccatcttaa aataactaaa acaaaaaaag tagtcgttgt taatcctcat taataatcgt
    28261 tttctttttt tcaaacgctc catttttatg tggaacatta tttttttaag gaactttgtc
    28321 aacatgtgga taactatttt ccacttgtgt cctggactag ctgttttgct gtaaaataca
    28381 aaacttgctg cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc gctggatcta
    28441 gatttggcaa agtcaagact gcatcatgta aattggcgtg gttgtccaaa accaaatcag
    28501 caaattcaaa taatcgcaaa ccactgctat gttgcgattt gatacttcgt gacaaatcaa
    28561 atccagtaac aatgattaat ggatgaccat tttctttgat ccactgagct agttcgatga
    28621 ttgccgggtt tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa
    28681 gctgatcaat aattgcctgt cctgcacctt ctagcgtttc atagacacct ccggctggat
    28741 tttcaatcaa tatacttggt acaagatccg gggtttgatc ccaaaattca aatgctacac
    28801 cacttagtgt gcggctagca tatatctgca atctaccacc ttgattaatt tgtttcgcga
    28861 tccaagtgcc agcttgaagc atcagcggct cttctatcct taatttctct tccaattggt
    28921 gataatagtc aaaataacat aaactcattt atctctcccg cctagctata cttgacatga
    28981 atagaaacag ctgcctaatc taaggaagta cataaaaaaa cagtagttag acagtaacac
    29041 atgtggtctt tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc caataccttg
    29101 acatagcaaa taaaacaacg tgcagaccat ggaaaatatc tacatagggg tagaattttc
    29161 accctagatt aggtacaata aattaggtcg atgcactgca ttgactatgg caaaatgatc
    29221 ggtcagggtg tatgtgtgtg actggtcatt ccctgattct tagcgccaac taagggtcag
    29281 ggagcctttt tttattcagc tgtttctatc tgaatttatt gtaagtaatc tcttatccaa
    29341 gtgcaagcaa gaaaatacaa aaaaacattg gtatgattta tatatatata aataacataa
    29401 aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata
    29461 agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt
    29521 attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact
    29581 ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg
    29641 agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc
    29701 aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac
    29761 ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat
    29821 gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag
    29881 ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac
    29941 acaccttttt ttataattaa aaaattcgct tttttattct actatgaatc ttctggcttg
    30001 gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat
    30061 tagcataata ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga
    30121 ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc
    30181 cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct
    30241 gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa
    30301 tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat
    30361 ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca
    30421 tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt
    30481 ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca
    30541 ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca
    30601 ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct
    30661 tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa
    30721 tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg
    30781 gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt
    30841 tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg
    30901 atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg
    30961 tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg
    31021 attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca
    31081 gcggcaggaa tgactgcttt tttgaccttc acttgct
//
LOCUS       DASHFF010000027        29572 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.027, whole genome shotgun sequence.
ACCESSION   DASHFF010000027 DASHFF010000000
VERSION     DASHFF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.027"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="U2I75_001413"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="U2I75_001413"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN1696559.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="U2I75_001414"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="U2I75_001414"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN1696560.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="U2I75_001415"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="U2I75_001415"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN1696561.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="U2I75_001416"
     CDS             complement(3376..4683)
                     /locus_tag="U2I75_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN1696562.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="U2I75_001417"
     CDS             complement(4686..5267)
                     /locus_tag="U2I75_001417"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN1696563.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="U2I75_001418"
     CDS             complement(5839..7131)
                     /locus_tag="U2I75_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1696564.1"
                     /translation="MKKKYVQVGLGGRARFFYETIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="U2I75_001419"
     CDS             complement(7128..7751)
                     /locus_tag="U2I75_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696565.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="U2I75_001420"
     CDS             complement(7754..8590)
                     /locus_tag="U2I75_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1696566.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="U2I75_001421"
     CDS             complement(8607..9494)
                     /locus_tag="U2I75_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1696567.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="U2I75_001422"
     CDS             complement(9510..10793)
                     /locus_tag="U2I75_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1696568.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDCLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="U2I75_001423"
     CDS             complement(11476..12159)
                     /locus_tag="U2I75_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN1696569.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="U2I75_001424"
     CDS             complement(12177..12794)
                     /locus_tag="U2I75_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1696570.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="U2I75_001425"
     CDS             complement(12804..13628)
                     /locus_tag="U2I75_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN1696571.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="U2I75_001426"
     CDS             complement(13621..14181)
                     /locus_tag="U2I75_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696572.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="U2I75_001427"
     CDS             14355..15311
                     /locus_tag="U2I75_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1696573.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="U2I75_001428"
     CDS             complement(15616..16572)
                     /locus_tag="U2I75_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN1696574.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16676..17278
                     /locus_tag="U2I75_001429"
     CDS             16676..17278
                     /locus_tag="U2I75_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1696575.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17366..18865)
                     /locus_tag="U2I75_001430"
     CDS             complement(17366..18865)
                     /locus_tag="U2I75_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN1696576.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18881..19405)
                     /locus_tag="U2I75_001431"
     CDS             complement(18881..19405)
                     /locus_tag="U2I75_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN1696577.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19703..20452
                     /locus_tag="U2I75_001432"
     CDS             19703..20452
                     /locus_tag="U2I75_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1696578.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20443..21228
                     /locus_tag="U2I75_001433"
     CDS             20443..21228
                     /locus_tag="U2I75_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN1696579.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21197..21913
                     /locus_tag="U2I75_001434"
     CDS             21197..21913
                     /locus_tag="U2I75_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1696580.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22015..23049)
                     /locus_tag="U2I75_001435"
     CDS             complement(22015..23049)
                     /locus_tag="U2I75_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN1696581.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23053..24111)
                     /locus_tag="U2I75_001436"
     CDS             complement(23053..24111)
                     /locus_tag="U2I75_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN1696582.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="U2I75_001437"
     CDS             complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="U2I75_001437"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN1696583.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25556..26392)
                     /locus_tag="U2I75_001438"
     CDS             complement(25556..26392)
                     /locus_tag="U2I75_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1696584.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26576..27793)
                     /locus_tag="U2I75_001439"
     CDS             complement(26576..27793)
                     /locus_tag="U2I75_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1696585.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(27942..28121)
                     /locus_tag="U2I75_001440"
     CDS             complement(27942..28121)
                     /locus_tag="U2I75_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696586.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            28176..28259
                     /locus_tag="U2I75_001441"
     tRNA            28176..28259
                     /locus_tag="U2I75_001441"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28210..28212,aa:Leu,seq:gag)
     gene            complement(28499..29497)
                     /locus_tag="U2I75_001442"
     CDS             complement(28499..29497)
                     /locus_tag="U2I75_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696587.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8844 a         6304 c         5063 g         9361 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgtctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaacaatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
    15421 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    16621 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    16681 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    16741 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    16801 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    16861 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    16921 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    16981 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    17041 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    17101 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    17161 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    17221 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    17281 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    17341 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    17401 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    17461 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    17521 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    17581 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    17641 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    17701 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    17761 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    17821 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    17881 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    17941 tgatttcttt ttcttccagt tcgatttgtt tttgacgttc tacgatttta atctgcattt
    18001 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    18061 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    18121 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    18181 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    18241 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    18301 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    18361 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    18421 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    18481 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    18541 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg
    18601 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    18661 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    18721 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    18781 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    18841 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    18901 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    18961 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    19021 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    19081 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    19141 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    19201 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    19261 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    19321 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    19381 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    19441 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    19501 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata
    19561 aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa
    19621 cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt
    19681 gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat
    19741 tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac
    19801 acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt
    19861 tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc
    19921 tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa
    19981 agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat
    20041 tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg
    20101 acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt
    20161 ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga
    20221 acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc
    20281 ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga
    20341 gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt
    20401 aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata
    20461 gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca
    20521 actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa
    20581 tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct
    20641 ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa
    20701 aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt
    20761 tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc
    20821 gattctttta tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta
    20881 gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag
    20941 gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat
    21001 gacggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg
    21061 aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt
    21121 ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca
    21181 aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc
    21241 agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag
    21301 aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga
    21361 tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa
    21421 tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat
    21481 ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc
    21541 gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa
    21601 attgatcgat caagtattca accacaccac tcccattcac gaaataccat cattcatcga
    21661 tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga
    21721 agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt
    21781 caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt
    21841 ggaaatcggc cggccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac
    21901 agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac
    21961 aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct
    22021 taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc
    22081 acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc
    22141 tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc
    22201 tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac
    22261 atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc
    22321 ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa
    22381 tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat
    22441 atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc
    22501 tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc
    22561 cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact
    22621 aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt
    22681 atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata
    22741 cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt
    22801 gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt
    22861 ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac
    22921 gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa
    22981 tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc
    23041 ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca
    23101 tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag
    23161 tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat
    23221 catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa
    23281 aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata
    23341 ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat
    23401 tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca
    23461 cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt
    23521 aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc
    23581 gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact
    23641 catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga
    23701 atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt
    23761 agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa
    23821 aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt
    23881 gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc
    23941 tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat
    24001 cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact
    24061 gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct
    24121 cctaattatg ttcatttatt cacaataaaa aaagctttta ttttgctaat acgtattaac
    24181 atttttaatt ttatctttac attgttactt attgaacaat aggaaattaa agaaactttt
    24241 gaaacttatg taaagtgatt tttactagaa cacaaaaaaa accttaaaac caaacttaag
    24301 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
    24361 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
    24421 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
    24481 tcatgactgc ttgttctggt gttgctaatc cccagtctac aacattttta attgcttctt
    24541 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
    24601 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
    24661 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
    24721 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
    24781 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
    24841 caaatacact tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta gcattactgt
    24901 gtcctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
    24961 gacgttccgg tgctaaagca atttttttga ttaaaccacc tgaagcttct tgccattcat
    25021 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
    25081 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
    25141 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
    25201 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
    25261 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
    25321 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
    25381 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
    25441 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
    25501 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
    25561 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
    25621 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
    25681 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
    25741 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
    25801 ttgatcgttc atcagctgat tgatgcagca gttggatgct tcgactcaaa tagtagcgtg
    25861 cttcagccgt ttttcctgcc acaccatata tttttccgat acaactaatc agttgtattt
    25921 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
    25981 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
    26041 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
    26101 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
    26161 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
    26221 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
    26281 gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc attttttgtt
    26341 cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc ataagtatcc
    26401 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
    26461 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
    26521 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
    26581 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
    26641 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
    26701 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
    26761 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
    26821 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
    26881 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
    26941 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
    27001 tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
    27061 ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca ggcgctaatg
    27121 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
    27181 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
    27241 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
    27301 tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
    27361 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
    27421 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
    27481 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
    27541 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
    27601 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
    27661 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
    27721 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
    27781 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
    27841 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
    27901 aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc atgatagtca
    27961 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
    28021 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
    28081 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
    28141 gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa ttggcagaca
    28201 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
    28261 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
    28321 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
    28381 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
    28441 taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt tttgtatatt
    28501 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
    28561 taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc attagtacta
    28621 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
    28681 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
    28741 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
    28801 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
    28861 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
    28921 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
    28981 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
    29041 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
    29101 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
    29161 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
    29221 attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa
    29281 tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat
    29341 tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc
    29401 ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat
    29461 agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt
    29521 atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DASHFF010000028        29108 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.028, whole genome shotgun sequence.
ACCESSION   DASHFF010000028 DASHFF010000000
VERSION     DASHFF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.028"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1028)
                     /locus_tag="U2I75_001443"
     CDS             complement(<1..1028)
                     /locus_tag="U2I75_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1696588.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1110..1799)
                     /locus_tag="U2I75_001444"
     CDS             complement(1110..1799)
                     /locus_tag="U2I75_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1696589.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1905..2273)
                     /gene="rplL"
                     /locus_tag="U2I75_001445"
     CDS             complement(1905..2273)
                     /gene="rplL"
                     /locus_tag="U2I75_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN1696590.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2326..2829)
                     /locus_tag="U2I75_001446"
     CDS             complement(2326..2829)
                     /locus_tag="U2I75_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN1696591.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3033..3160)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3239..3928)
                     /gene="rplA"
                     /locus_tag="U2I75_001447"
     CDS             complement(3239..3928)
                     /gene="rplA"
                     /locus_tag="U2I75_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN1696592.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4032..4454)
                     /gene="rplK"
                     /locus_tag="U2I75_001448"
     CDS             complement(4032..4454)
                     /gene="rplK"
                     /locus_tag="U2I75_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN1696593.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4797..5465
                     /gene="sdaAB"
                     /locus_tag="U2I75_001449"
     CDS             4797..5465
                     /gene="sdaAB"
                     /locus_tag="U2I75_001449"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN1696594.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5478..6350
                     /gene="sdaAA"
                     /locus_tag="U2I75_001450"
     CDS             5478..6350
                     /gene="sdaAA"
                     /locus_tag="U2I75_001450"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN1696595.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6441..7460)
                     /locus_tag="U2I75_001451"
     CDS             complement(6441..7460)
                     /locus_tag="U2I75_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1696596.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7633..8538)
                     /locus_tag="U2I75_001452"
     CDS             complement(7633..8538)
                     /locus_tag="U2I75_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1696597.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="U2I75_001453"
     CDS             complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="U2I75_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN1696598.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9243..9413)
                     /gene="secE"
                     /locus_tag="U2I75_001454"
     CDS             complement(9243..9413)
                     /gene="secE"
                     /locus_tag="U2I75_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN1696599.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="U2I75_001455"
     CDS             complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="U2I75_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1696600.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9751..10395
                     /locus_tag="U2I75_001456"
     CDS             9751..10395
                     /locus_tag="U2I75_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1696601.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10451..10954)
                     /locus_tag="U2I75_001457"
     CDS             complement(10451..10954)
                     /locus_tag="U2I75_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1696602.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10967..11013)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11551..12516
                     /locus_tag="U2I75_001458"
     CDS             11551..12516
                     /locus_tag="U2I75_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1696603.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12615..13787)
                     /locus_tag="U2I75_001459"
     CDS             complement(12615..13787)
                     /locus_tag="U2I75_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696604.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13774..14919)
                     /locus_tag="U2I75_001460"
     CDS             complement(13774..14919)
                     /locus_tag="U2I75_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696605.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14933..15865)
                     /locus_tag="U2I75_001461"
     CDS             complement(14933..15865)
                     /locus_tag="U2I75_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1696606.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15982..17061
                     /locus_tag="U2I75_001462"
     CDS             15982..17061
                     /locus_tag="U2I75_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1696607.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17058..17687
                     /locus_tag="U2I75_001463"
     CDS             17058..17687
                     /locus_tag="U2I75_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1696608.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17797..18024)
                     /locus_tag="U2I75_001464"
     CDS             complement(17797..18024)
                     /locus_tag="U2I75_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1696609.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18127..20715)
                     /locus_tag="U2I75_001465"
     CDS             complement(18127..20715)
                     /locus_tag="U2I75_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN1696610.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20735..21394)
                     /locus_tag="U2I75_001466"
     CDS             complement(20735..21394)
                     /locus_tag="U2I75_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1696611.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="U2I75_001467"
     CDS             complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="U2I75_001467"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN1696612.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22167..23033)
                     /locus_tag="U2I75_001468"
     CDS             complement(22167..23033)
                     /locus_tag="U2I75_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1696613.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23142..23783
                     /locus_tag="U2I75_001469"
     CDS             23142..23783
                     /locus_tag="U2I75_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN1696614.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="U2I75_001470"
     CDS             complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="U2I75_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN1696615.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25224..26123)
                     /locus_tag="U2I75_001471"
     CDS             complement(25224..26123)
                     /locus_tag="U2I75_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1696616.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26125..26922)
                     /locus_tag="U2I75_001472"
     CDS             complement(26125..26922)
                     /locus_tag="U2I75_001472"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN1696617.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26900..27604)
                     /locus_tag="U2I75_001473"
     CDS             complement(26900..27604)
                     /locus_tag="U2I75_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN1696618.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27735..28310
                     /locus_tag="U2I75_001474"
     CDS             27735..28310
                     /locus_tag="U2I75_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEN1696619.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28428..29105
                     /locus_tag="U2I75_001475"
     CDS             28428..29105
                     /locus_tag="U2I75_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1696620.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6169 c         5232 g         9142 t
ORIGIN      
        1 acagtattat ttcggttgct tgctgcttca cctagccctt gtactcgtgt ttgattggcg
       61 acttgaccaa attgattccc gacgtctaat aaaataatag ctaaaagcaa aatggccaaa
      121 tggcttccgc caaccaataa agcaataaag cttattgttt gcataacaag tcccatatac
      181 acgatttttc tttcagaata agaatctgcc agtcgtccca cgataggtgc agcaactgct
      241 ccagacaaac caaaaatcgc taatatccct gcttcgaatg ttccccaatt gtaggcggga
      301 ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa atgttccaaa catgaaaaat
      361 ccactgactg ccgcttctct taatagacgt tgagaagcaa ctaaatgcgg caaactttta
      421 agtgaatcaa ggtatgaaac agctgtacta ctttgctgga cagaacgcgg cattttccag
      481 taaagcagaa gtgtaagaat tccgactgca cataaagcaa ataaatagat catccgccaa
      541 gaaaaaagac tggcaacaat accagatacc gtccgagaaa gcaaaatccc cacgagtaaa
      601 ccgctaagca atgtacccat gaccttccct cttgcttttg gtcccgctag taccgcaccg
      661 taaggaatga tgatttgcgg cacgatcgaa agtaatccta ttaaaaaaga agcgcatgca
      721 aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag agacggcaga taacgctacc
      781 attcgaatga tcaattttgg acgatccacc acgtccccta aaggaactaa aaacagtaac
      841 cctaacgcat agcctaattg ggtcaacatc gtcaatatcc cgactgcact tgtgctaaca
      901 gataaactag ctgctacttt tgttccaatc ggctggatgt aatacatatt ggcaacaaca
      961 acgccacaag tgattgataa tagtaaaata agcgaagttg ttaaaggtgt tttttcatta
     1021 ctcttcattg atttccccca tttcctatct tagtttacaa cttatggaaa agattggaaa
     1081 tagaaaaata caactattcg ccacacttat tatttgtgct tttgttttcc ttgttttttc
     1141 ttgcgatctt tataaatgaa gacaactaac cctactagca cagtaacact tgcaaccaac
     1201 gtgactgcag ttttgttata tccgtgaaac agagcttctc caccacacgc atacaacaca
     1261 gacgaaggga tgactccaat cacgatcgac aatatgattt gctgtagagg caatttcatc
     1321 gcatctgcag caaaattgac catagaagat ggaataaccg gtatcatata gcctaacatt
     1381 acacctattt ttggatgttt catttgtcgg attgcttgca cccaatgatt cgtttcggtt
     1441 ttcttatcta aaaattttaa gcgatgcata aggaaaattg cagtgatgtt accaagagca
     1501 ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa agctcaaacc agcaatgact
     1561 ccaactactg aagtaggaat acctggaaca gcacacatca ccgctgtcaa tacgatcaag
     1621 agtatcgatg caaaagtccc atgcgaacga atatgtgcga gtagctctgc cttactcgct
     1681 ctcggattta aaaataattg gatgtctgtc tgatattcta aatacaaccg atagcccact
     1741 accagtatca gaatgattcc caacgagatc aacatcaatc ggattatttt ctttttcata
     1801 tttcctcctg aaaaaaatct atattcatta aataatagtt taacataaaa acaaaaaagc
     1861 accgaaatcc aacataggat ctcagtgctt ttaaatagta aaaattattt aacagttaca
     1921 gatgcgccaa cttcttcaag tttagctttc aattcttcag cttcttcttt agatacgcct
     1981 tctttaactg gtgcaggagc gccatcaact acagctttag cttctttcaa gcctaaacca
     2041 gttgcttcac gaactgcttt gataacttta actttttggt ctccagctgc agttaattct
     2101 acagtgaatt ctgtttgttc ttcagcagca gcagcgccac cagcagcagc tacagctaca
     2161 ggagcagcag cagatacgcc aaattcttct tcaatagctt taactaggtc gttaagttct
     2221 aggatagttg cttcttttaa ctcagcaacg atgttttcaa tgttcaatgc cattataatt
     2281 tcctccgttt aattgttctt ttttatttaa tgctttatgc ggcaattaag ctgcatctcc
     2341 atcttctttg tctgccactg ctttgacagc gtaagccacg ttgcggactg gcgcttgtag
     2401 tacagatagc aacatagata gaagtccttc gcgatttggc aattttgcca atgctgtgat
     2461 ttcttccaat gaagagactt taccttcgat gataccgcct ttgatttcta atgcttttgc
     2521 ttctttagca aattcgtcca tgattttcgc tggtgcgact acatcttcgt tactgaatgc
     2581 aacagctgta ggtccagtga atacttcgtc catgccttca aggccagctt ttttagctgc
     2641 acgtgaaaga atagaatttt tgataacttt catttctacg ttagcatcac gaagttgttt
     2701 acgtaaacgt gttacttcat caactgttaa tccgcggtag tcaacgacta ctactgatgc
     2761 agctgctgta aatttttcgc ttacttcatc aacgattgct gctttttttg cgataattgc
     2821 ttcactcatt tttgagtttc acctcctgaa ttttgatgga agagtttttt gcactgaaaa
     2881 ggcaagtcac ttttatagtt actttgctca accagtatta ttcatcacct attcacttcg
     2941 ttcatagtga ttctactcgc tttgctcgaa aggaaactcc tttcgctttg ctcaaccagt
     3001 attgttcatc atctcttcac ttcgttcata gtgattcaca ataaaaaact ctatgccacc
     3061 gcagacatag agggactatt gaatatattc aataaacgtc ctcggtagga aattaaggct
     3121 tgatgccacc tactgtcttc ggtacaagtt aattattaac ctcaactagc ttagcatgct
     3181 aaactagttg agtcaagaat tatttttcag ttggatcaat caacattcta atatttaatt
     3241 agaaagaagc ttggtcaaca tggataccag gtccaaatgt tgttgtgatc gttacatttt
     3301 tcatgtattg acctttagct gctgatggtt tagcttttag caatacatca tggattgctg
     3361 caaagttttc aactaatttt tcatcgtcaa atgaaacttt tccgattggt acatggatat
     3421 ttccagcttt gtccacacgg taagttactt taccagcttt cacttcgttt actgcttttg
     3481 ttacgtccat tgtaactgta ccagttttag ggtttggcat taagcctttt ggtcctaata
     3541 ctcgtcctag acgacctaca gtagccatca tatcaggtgt tgcaacaact acatcaaagt
     3601 caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag
     3661 ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac
     3721 cagttccgtt tggtaataca acagcaccac ggatttgttg gtctgctttt ttagggtcta
     3781 cgttcaaacg gtatgcaact tcaactgttg catcaaattt tgcaatgttt gtttcttttg
     3841 ctaaagctac tgcttcggca acaggataaa ctttacttga atcaactttt tttaatgcat
     3901 cttgcatttt tttgctcttt ttagccattt tggttcctcc ttgattgtgg ttataacggt
     3961 tgtacctccc acgtgttcta tatctttcaa tatagagcca actgagaagc tgctgtctta
     4021 gggtgtatag attattctac agtgatcccc atgcttcgtg cagttccttc aaccatgcgc
     4081 attgctgctt caacgttagc tgcgtttagg tcttccattt ttaattcagc gatttctctt
     4141 acttgatcgc tagaaacttt cgcaacttta tttttgtttg gttcgcctga acctttttcg
     4201 attttagctg cttttttcaa tagaacagca gctggcggtg tttttgtaac gaatgtgaat
     4261 gaacggtctt cgtatacaga aataacaacc ggaatgatca aacctgcttg atcagctgta
     4321 cgagcgttga attcttttgt gaatcccatg atattgatac ccgcttgacc tagtgcagga
     4381 cctactggtg gagctggagt tgctttacct gcaggaattt gcaatttaac gattttttct
     4441 actttctttg ccacgagaca tacctcctta agtccgtgat gtggttaatt ggagatgcat
     4501 atttctcctc ccacgtttta tctgatctga aaaaatccag acaaataatg tgcaaaacaa
     4561 tgcacactgg tacagtatag caaatttatt tgttttttca agattttttt ctgctatttt
     4621 tacattttgc ttgaataaac gctaaattta ttctaaaaaa acaacaattt ttcgttctca
     4681 tttcttcttt tatgttacac ttgcgctagc atggttcaac agatgaaagc aaacaataac
     4741 acggtttttt agacccttgt acaaagattc ctacataaca aaatgaggtg gagaaaatga
     4801 aagatttaag gtatagaagt gtctttgata ttattggacc tgtgatgatt ggtccaagta
     4861 gctctcacac tgccggagct gctagaatcg ggaaaatcat acgtagtata tttggcgaac
     4921 agcctgattc tgtagatatt tatctatatg aatcatttgc aaaaacctat cgggggcacg
     4981 gaacagatat tgcattagtt ggcggcctgt tagggatgga acccgatgat gaacgtttag
     5041 ctgattcttt gaaattggca tatgaagccg gtatggaagt cttgtttgtt cctaagagtg
     5101 aaaaagccga acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt
     5161 cagtcactgg catttcgatc ggcggtggca atatccagat ttctgaattg aatggattca
     5221 aaatttcttt aagcatgggt actcctacct tgatcgttgt ccaccaagat gttccaggta
     5281 tgattgctaa agtgactagt atactttctg agaccaatac gaatatcggc actatgacag
     5341 tgacacggga atcaaaaggt gaaaatgcca ttatgataat tgaagtagat gatcctcaag
     5401 tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta aattatttcg
     5461 attaaggagc taaaaaaatg tttttatcga ttgaagaatt agtagatcaa gcaaaagctt
     5521 atccaagtgt agcagaattg atgattgcag ttgaaatgga aatgagtggc cgttcccgta
     5581 gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg
     5641 gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg
     5701 attatttaaa tgaggcagac tttttaagtg gagaaacaat cttaaaagct gtacgcaatg
     5761 ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg
     5821 gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg agatcggctt gatttgacac
     5881 atgaacagca attggaattc ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca
     5941 acgcttctat cagtggtgca gaagggggat gtcaagcaga agttggttct gcaagtgcaa
     6001 tggcagcagc agctttggtt tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag
     6061 tagccattac attgaaaaat atgatgggac tgatttgcga tcccgttgca ggcttagttg
     6121 aagtcccgtg tgtcaaacgt aatgctttag gctcttctca agcttttatc tctgctgata
     6181 tggcattagc gggtatcgaa agtgtcattc cgccggatga agtcgttgct gcaatgtacc
     6241 aagtcggacg tcaaatgccg tcgattttca aagaaacagc agaaggtggc ttagctgtta
     6301 cgccaactgc taaacgactt gcggaagaga ttttaaataa gagggcgtga taaagctcct
     6361 taatttcgag tattaagaga ttagttatct tcataacaaa aaacgaagag aggactaatt
     6421 gcccttctct tcgtttttta ttaatccatc aatttcgtat tttttacttc atcgatcgtt
     6481 tttgttcctg caagttgcat cgtgatcgac aattctttgt tcaggtgttc aaaaacagat
     6541 gttactcctt ctgccccacc tagattcaac ccataaatca ctggtcggcc aatcgcaact
     6601 aagtcagcac cacttgccag agctttgaag acatgctctc ctcgacggac accagagtcg
     6661 aaaatcactg gtacacgttt gttgactact tcggcaattt ttggcaatac ttcaaaggac
     6721 gcaggacctc catctaattg acgtccacca tgattggata cccagatacc atctgcccct
     6781 gcagagatag cgattactgc atcttctggc gattgtattc cttttacgat aactggaaga
     6841 tgggtgattt ctttgattgt tttgatgtca tctggtgtca agccttgttt tgcagctgca
     6901 taaatttctg cgattccttt tccttcgcca ttaccacttg cactttgttc gctatacgca
     6961 gctaagttag gcattggtaa tggaaattgg aattgattga tgacgtcttc ttcacggtaa
     7021 ccaccgagcg ttgaatctgc cgttaaaatg atagctttag cccctgcttc tactgctttg
     7081 ttcaaaatga actcattgaa tctgtcgtct ttactcatat ataattggaa aaactgcggt
     7141 gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt gctgatggaa
     7201 aagattgatc cagcagcagc tactccttta gctgtatcag cttccccttt ttcatgtgcc
     7261 aatccttggg ccgctgatgg cgcttggata atcggtgtat ctaattcaat tccgaaaaca
     7321 gatgtatgca aatcggctga gtcgattccg cgtaatatcc gtggcatgat tttctttgta
     7381 ttaaaagaag tcgtattttc tttcatcgtc cactcatctt cagaaccacc gcgaatatag
     7441 ccaaaagctc ctgcttccat gcgctctttt acacgacctt ctagttaggc aatgtttaca
     7501 atatcgattg gatgttcttc attacttgta tgataagtca tagtagatcc ttctttcatc
     7561 ttcttgatta catataaatc atactaaact tacaaaaagt aagcaaacat ttactctcag
     7621 atacttttat cgctattctt tccaaataaa tcgacttaca agctgatcta cttcttggtt
     7681 ttcatgaagc atactatgag aggcattttc acctgtcact actttttcct ctatttcatt
     7741 tccgcgttgc ttcaacaacg catagaccgc taatgcactg tttaacggta ctgttccatc
     7801 actcaagtct gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg ttataggaat
     7861 attaccgatc atttgctggt aatcttgata gcggttagaa acgacagcag gcccctttgc
     7921 taactcatct gtcagagact gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat
     7981 tgctgttaat gtctgaactt gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg
     8041 tagtgcacta actcctccca tggaatgccc cactatgttg actttatcta tgccgtagtt
     8101 ttctttcaga tacaaaagac aagttttgat ccattctgct tgattccact cgttattttt
     8161 attatcttca aacaatactt ggaccatcgg attgtctgct tttccagaca aatctcctgt
     8221 tgcttgtatt tgtccatctg ttcctactgt taaaaccaat tcttttactg cttgctgatt
     8281 tttttccaaa cgccgcaaca tccctttaaa ggaattttct gtcccgctgt agccgtggaa
     8341 aaaaagtgtt ggaatagtgg ttttttctat cactttactg gattctgccg ttttttcttt
     8401 atcagaaact tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg caaacaaact
     8461 gataaggact aaagctgctg cagttgcacg tttgcataaa tttttgattg gttctgtcat
     8521 acacctcttc ctttccatat agaaaaaaga aagacttgaa aggccaatca tatgatcgac
     8581 atttcaagtc tctttattaa tcgattttat ctacttgatc gaaatcaagt tctgtactgg
     8641 tttcacgtcc aaacatatcg atgttgactt tcaatttttc tttttctccg tcaacttctg
     8701 ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat tttcacgact tcgccaactt
     8761 ctacttctag atcattttgt cgcaggctca taccgataga acgcaagata tgattgactt
     8821 cttctggaag taatggtgct ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca
     8881 caccaggtgt gttgcggaca acataccatg aagcgtctgt catgatcatt tcaacaagta
     8941 cgtatcctgg gaatgtttta tgaacgattt ctttttcttt cccatttttg acttcagttt
     9001 ctttttcttc aggaacaacg acacggaaaa taaagtcggc cattttcata ctttgcgcac
     9061 gagattcgat atttgctttt actttgtttt catatcctga atatgtatgc aagacatacc
     9121 agtttttttc aaaagattcc attgaaggct cctcatttca ttgtaaaata aaaaaacctc
     9181 agtacacccg aagtttttct ttcgcttcat tatagcacta aaagtacgtt gtggctagcg
     9241 agttatttta aaatccagcc aaataagctt tggatggctg tatccattac gtaaaacatc
     9301 aatccgaaaa taatagaagt ttcgatgact actaacgtat ctttacggag ttgttttctg
     9361 cttggccaag ttacttgttt gatttcatct tttacgcttt ttagaaattt catgagacaa
     9421 cctcccttgc tactgtttat ttcgtttctt tatgtaatgt atattgatta caatatttac
     9481 aaaacttatt gatttccaaa cgttcgccgc tttttccttc actgactgat tttgtgtagt
     9541 tgcgagaccc acaaacggaa caagcgagtg ctgctttttt ctttgccatt tacttctgcc
     9601 cccttgctga gaatcagact tatacttcat aacagtatca cgtaactttt tacttgtcaa
     9661 tttgtgaaat aaactatcta ttgttttgga taaaattatg atatgattaa tttgaaatta
     9721 agctaaaatg agacaattgg aggtatagcg atgctattaa aaacacttgt atataagaaa
     9781 aaagatctga caaccgttac agagactagt acattagaag aagcattaaa aattttagaa
     9841 acttccggat accgttgtgt gccgatctta gattcttcag gtaatatttt ccgtggaaac
     9901 atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc
     9961 actcatcttt tgaaaaacgc tacaaagttc atttacctgg atacttcttt cttcaaagta
    10021 ttctttacga tcaaagagct tccttatatt gctgtccttg acagtgaaaa tcatttttat
    10081 ggtattttga cacatagtac gttgcttaat atgctctctc aatcttggag cgtagaacaa
    10141 ggaagctatg tactgacaat tgcttccaca ggaaagcaag gagatttggc tgctattgca
    10201 aaaatcattt caaaacacag cagcattgcc agctgtatta ctcttgatat cgaaaaagac
    10261 gaatacattc gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa
    10321 acgatcgtca accacttaga gaaaaagaat tataaagtgg tagaaattga aaatttgcaa
    10381 aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc taatctgctt tcttttttcg
    10441 ctttttataa ttagacacgc ttttcaaaat caagcgcgcg atctaaataa tacatcaatg
    10501 gtgctgaaac agccatgacg ataaactcac ttaatgcaag cgtgccatag gttgtccaaa
    10561 atgcttggcc acctgttgga actaacatat aagcaatcag tcccatgctg atggtaaaga
    10621 agatcgtgtt caatactaaa cgcatcttta catttttcac tttgttttgc aataaagctg
    10681 tcaggcctaa agaaagcaat gtttggccgc caccaaaaac cacatccatg ataccaaaac
    10741 caaagaaggc attatagacg atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa
    10801 agacaactaa atgattcaaa ctttctgaaa tacgaaactg gatcggccct gaggatacag
    10861 gcgcaactaa aatagttaac gctagataaa gagccatgat gatgccattt acaacgatta
    10921 cggataaccg ttgtctgctt ttcgtttttt gcattttctc tcctcttttc tcctagtttt
    10981 tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac aaacaataat tataacagac
    11041 ttttcattta tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta
    11101 aacgtttgct aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc ataagaattc
    11161 ccagtaaagc agacgaagta ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat
    11221 aaaaaaggat gtgggaattt gagcaacctc ttactagaga acggtctagc aagcagctga
    11281 agatctaagt ggacatgccg aaccttggaa cttctcctta cgtaagtaat ggatcatgcg
    11341 cttgctaggc ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg
    11401 ctataataac caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat
    11461 tcaaaaaata gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata
    11521 tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat
    11581 ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa
    11641 aataagcata ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt
    11701 gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc
    11761 gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat
    11821 actttctatc agatggaagt agaattccca gttgcttata ttccagcagg ttctggaaat
    11881 gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta
    11941 gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa
    12001 aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca
    12061 aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta
    12121 ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga
    12181 ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt
    12241 ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc
    12301 gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa
    12361 aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca
    12421 acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc
    12481 acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta
    12541 catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt
    12601 tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta
    12661 cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg
    12721 aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta
    12781 ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag
    12841 tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg
    12901 atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg
    12961 atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag
    13021 gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa
    13081 ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg
    13141 tttcgtttac tgatctcttt gcgattaaac gctagattga tgacgctgaa accgctaaaa
    13201 atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc
    13261 atccgctgac tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt
    13321 gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc
    13381 aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt
    13441 atcttgatcg tcgccatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt
    13501 aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca
    13561 tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc
    13621 gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca
    13681 atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc
    13741 gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct
    13801 ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt
    13861 ggtataaaaa ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa
    13921 gttgacgaga tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg
    13981 ttccgacccc atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct
    14041 atccttttgc accagattca tttttggtat caaattacca accaatgtac ctaacaaaat
    14101 cgcacacaac gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc
    14161 tacttgatag ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt
    14221 aaagcttgct gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa
    14281 gcgaatacca tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat
    14341 cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat
    14401 attacgacct gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg
    14461 cgcgagatca acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa
    14521 aaattgatcc atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag
    14581 cgaaacagat gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc
    14641 attcgcttct acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac
    14701 ttgttctaag actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat
    14761 tccagtatta atcgtttcaa gattgccacc ttgatcaatc tcgccaaagg ccgccataaa
    14821 catcaatcct aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag
    14881 cagtattttt gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc
    14941 tcaaatcctt gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta
    15001 tttttccgta actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat
    15061 ttagttcagt agttgttact tggagctgac cttttttata agcgattcca tcagaagcta
    15121 ttgttcgctg aatcgctgac aacacatttt ctgggagtaa tgggatgtcg atcgtcaaac
    15181 gatccgccgt ccgtaccata tcttttagag attctttggt tcctttagca atcacctgtc
    15241 cttgatccat gatcacgatc tggtcgcaaa gaatttctac ttcttccatg taatgtgttg
    15301 tatagacaat ggttgctcct tgtcgattca gctccttgat actttctaaa attttattcc
    15361 gactttgggg gtctacagct actgtcggct catctaaaaa aattaaccgc ggcttgtggg
    15421 caatcccaca tgcgatgttc aaccgacgtt tcaagccacc actaagttct cctggacgaa
    15481 acgaaacata tttttctaat cccactaatt gaatcacctc ttcaatcaag gctttccttt
    15541 tctctttttc ggaaacatat aaaccgcaaa aataatcgat attttcttgc acacttagtt
    15601 cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat tgccttgaca gcataatttt
    15661 caggagtcat tgttgtcccg aacaatttaa tggttccttt gtcataattt aacaaagaaa
    15721 gcatacagtt gatggcagtc gatttcccac tgccgtttgg acctaacaga cctaagactt
    15781 ctccttcttg tacttgcaga tcaaaatgat tcaatgctac aaaatctcca tagcgtttga
    15841 ctaatttatc aatttcgata atcatcatga aaaactcctt ccaaatttct tgttgatctg
    15901 actataataa agtgcaagaa aagaaactag tgacgattgg ctaaaccaca catgacattt
    15961 gtcacaatga aggagtctgc tatgcataaa tctcggctac tcaacgaaac tagctttcta
    16021 ctggttatct cattgctttt gctggtaagt caaggattcg ttgctatgca agttatctat
    16081 ttattgatcg ccgttttatt cactggtgtc agttatttac tgcctacaaa gccaaattgg
    16141 attttccaag ttattctatt tcttttatgt gcctttttac ctgatcttgt cctgtttctg
    16201 ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc ttgattactt gtttttgtta
    16261 ggttttttgt tgatttcgac ggttggtaat catcccccca tcaaggtcgc ctttgtcgta
    16321 attttgctgt ttttttcctt ttacttacga aaaaaagatc aacgaacgac cgatcttgag
    16381 aaaaagtaca ttgtctccaa agatactcat tgggaaaaac aacaagagtt atcggaacaa
    16441 aataaagcat tggccctgtc acaagaaaca caattagcct tgcaaattgt ggaagaacgg
    16501 aaccgaattg cccgagacat ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta
    16561 caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac taaaagaacc attagaaaat
    16621 ttaagtgcca ccgttcacac tgggatggat cggattcgtc aaagtgtcca tgacctccat
    16681 gagacatcga tctcttttca acactcctta gagtttctga tcgctgattt tccttcttct
    16741 gttacgattg aaggaaactt gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta
    16801 atggtcatca aagaagcctt aaccaacagt atcaaacata gtcatgccaa aaccgttgtt
    16861 ttacattttc gaacgttgcc ggccttttat cgcttgcaaa tcacgaatga tggcaccagc
    16921 ccacaaaaaa attctgctcc cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa
    16981 caaaagggac aagtccatat tttccaagaa aaccagcaat ttcagttaac gatcatttta
    17041 ccaagggagg gacaccaatg ataacagtag tcattattga tgatgatcca tttgtgacga
    17101 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
    17161 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
    17221 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
    17281 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
    17341 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
    17401 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
    17461 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
    17521 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt
    17581 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
    17641 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
    17701 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
    17761 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
    17821 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
    17881 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
    17941 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
    18001 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
    18061 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
    18121 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
    18181 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
    18241 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
    18301 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
    18361 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
    18421 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
    18481 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
    18541 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
    18601 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
    18661 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
    18721 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
    18781 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
    18841 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
    18901 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
    18961 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
    19021 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
    19081 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
    19141 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
    19201 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
    19261 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
    19321 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
    19381 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
    19441 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
    19501 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
    19561 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
    19621 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
    19681 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
    19741 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
    19801 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
    19861 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
    19921 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
    19981 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
    20041 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
    20101 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
    20161 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
    20221 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
    20281 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
    20341 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
    20401 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
    20461 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
    20521 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
    20581 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
    20641 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
    20701 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
    20761 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
    20821 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
    20881 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
    20941 cgaataaaag tgggccatct ttatgtcggg caaccgcatc tgctacaaca gtttcgatac
    21001 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
    21061 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
    21121 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
    21181 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
    21241 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
    21301 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
    21361 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
    21421 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
    21481 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    21541 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    21601 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    21661 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    21721 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    21781 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    21841 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    21901 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    21961 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    22021 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    22081 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    22141 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    22201 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    22261 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    22321 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    22381 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    22441 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    22501 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    22561 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    22621 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    22681 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    22741 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    22801 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    22861 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    22921 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    22981 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    23041 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    23101 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    23161 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    23221 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    23281 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    23341 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    23401 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    23461 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    23521 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    23581 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    23641 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    23701 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    23761 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    23821 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    23881 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    23941 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    24001 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    24061 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    24121 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
    24181 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
    24241 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
    24301 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
    24361 tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
    24421 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
    24481 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
    24541 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
    24601 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
    24661 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
    24721 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
    24781 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
    24841 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
    24901 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
    24961 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
    25021 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
    25081 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
    25141 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
    25201 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
    25261 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
    25321 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
    25381 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
    25441 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
    25501 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
    25561 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
    25621 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
    25681 aatccatgct ttgcaaaaag catcaagcca gatgtatccc gatctagtcg cgtcaccaca
    25741 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
    25801 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
    25861 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
    25921 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
    25981 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
    26041 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
    26101 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
    26161 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
    26221 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
    26281 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
    26341 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
    26401 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
    26461 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
    26521 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
    26581 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
    26641 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
    26701 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
    26761 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
    26821 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
    26881 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
    26941 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
    27001 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
    27061 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
    27121 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
    27181 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
    27241 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
    27301 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
    27361 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
    27421 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
    27481 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
    27541 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
    27601 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
    27661 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
    27721 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
    27781 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
    27841 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
    27901 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
    27961 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
    28021 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
    28081 taatccaaac cggaaagctt acaacaaaac gagctgaatt ccctattgaa gaaggacttt
    28141 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag
    28201 gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt
    28261 atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca
    28321 ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa
    28381 agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct
    28441 atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat
    28501 ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt
    28561 tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc
    28621 ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg
    28681 cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg
    28741 gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg
    28801 gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt
    28861 ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca
    28921 attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt
    28981 ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac
    29041 atttacatac aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta
    29101 attaaaaa
//
LOCUS       DASHFF010000029        27914 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.029, whole genome shotgun sequence.
ACCESSION   DASHFF010000029 DASHFF010000000
VERSION     DASHFF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.029"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(18..710)
                     /locus_tag="U2I75_001476"
     CDS             complement(18..710)
                     /locus_tag="U2I75_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1696621.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="U2I75_001477"
     CDS             705..971
                     /locus_tag="U2I75_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1696622.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="U2I75_001478"
     CDS             complement(1147..1716)
                     /locus_tag="U2I75_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1696623.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="U2I75_001479"
     CDS             complement(1790..3079)
                     /locus_tag="U2I75_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696624.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3433..4281
                     /locus_tag="U2I75_001480"
     CDS             3433..4281
                     /locus_tag="U2I75_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN1696625.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4426..5367
                     /locus_tag="U2I75_001481"
     CDS             4426..5367
                     /locus_tag="U2I75_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN1696626.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="U2I75_001482"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="U2I75_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="U2I75_001483"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="U2I75_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN1696627.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="U2I75_001484"
     CDS             complement(9831..12017)
                     /locus_tag="U2I75_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN1696628.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="U2I75_001485"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="U2I75_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN1696629.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="U2I75_001486"
     CDS             complement(12239..12679)
                     /locus_tag="U2I75_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN1696630.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13292)
                     /locus_tag="U2I75_001487"
     CDS             complement(12753..13292)
                     /locus_tag="U2I75_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEN1696631.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13907..15373)
                     /locus_tag="U2I75_001488"
     CDS             complement(13907..15373)
                     /locus_tag="U2I75_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1696632.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15684..17765)
                     /gene="cadA"
                     /locus_tag="U2I75_001489"
     CDS             complement(15684..17765)
                     /gene="cadA"
                     /locus_tag="U2I75_001489"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1696633.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(18289..18648)
                     /locus_tag="U2I75_001490"
     CDS             complement(18289..18648)
                     /locus_tag="U2I75_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN1696634.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18678..19019)
                     /locus_tag="U2I75_001491"
     CDS             complement(18678..19019)
                     /locus_tag="U2I75_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696635.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(19016..19690)
                     /locus_tag="U2I75_001492"
     CDS             complement(19016..19690)
                     /locus_tag="U2I75_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1696636.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20743..22041)
                     /gene="asnS"
                     /locus_tag="U2I75_001493"
     CDS             complement(20743..22041)
                     /gene="asnS"
                     /locus_tag="U2I75_001493"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN1696637.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(22081..23271)
                     /locus_tag="U2I75_001494"
     CDS             complement(22081..23271)
                     /locus_tag="U2I75_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1696638.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQT
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23292..23819)
                     /locus_tag="U2I75_001495"
     CDS             complement(23292..23819)
                     /locus_tag="U2I75_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN1696639.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23866..26655)
                     /locus_tag="U2I75_001496"
     CDS             complement(23866..26655)
                     /locus_tag="U2I75_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1696640.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26802..27002)
                     /locus_tag="U2I75_001497"
     CDS             complement(26802..27002)
                     /locus_tag="U2I75_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1696641.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27454..27774)
                     /locus_tag="U2I75_001498"
     CDS             complement(27454..27774)
                     /locus_tag="U2I75_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696642.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         7845 a         5917 c         4904 g         9248 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttccttcttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa atgccagata
    12901 aaagataaaa attctaagta ttccctgttt agtttctctg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaat
    13081 gtagaagtat aatttccttc aacaatccat gacggattag ataccataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct
    13201 agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt cgattttccc
    13261 gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc tttggaattt
    13321 ggttcgatca taccacttat aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt
    13381 caaagtccct tgtttcgaat aaatggtgtt caatcagctg accttcagca ttaactcaaa
    13441 aaacggcaaa atatgaggag ctattttttc gtttgttcct ctaatactca ggtcaaatcg
    13501 ctgattttac aacctcttcc ttaaacggtg ttctcctgcc aacacctcgg tcacaaggca
    13561 caatagtcaa caaacatgag aagttgtttt ttgaaattgc tccttgtgac tcggagttaa
    13621 acggcaggct cactacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    13681 gcccagcaaa acgaaaaata tgaggggcta tttttgtttg gctgttctta ttacttgatg
    13741 caggacagct ttttcacaac ctctgattca cggtgttcca gaagtagctt ctgcgaaaaa
    13801 caagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    13861 aagctgacca cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
    13921 ctgtttctgg ttcagtgatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
    13981 tacctaataa gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg
    14041 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
    14101 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
    14161 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
    14221 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
    14281 tatctttgat accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
    14341 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
    14401 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
    14461 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
    14521 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggaa
    14581 tattatcgct agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
    14641 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
    14701 tgacataagg ggatattttt tccgctcctc caactgcaaa tactagcatc gaaacggttg
    14761 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
    14821 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
    14881 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
    14941 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
    15001 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccgcca gcaatcagga
    15061 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
    15121 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
    15181 tatccttgaa ggctgaccct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
    15241 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
    15301 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
    15361 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
    15421 ttcaattttt aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat
    15481 acacatcgtt ttttcatatt ttttccatca aaaaaataag ttattttctt ttatttcatt
    15541 taccttattg aatttcaagc tattttgaat tttctgcaaa attaaaaaca ttagttattt
    15601 tataatccta tctattttct ttacattttt cattaagaaa aggatataaa aaaaagagga
    15661 cgttcattcg tcctcttcat tgatcaacgt ttatttttta catgcaagct tcgcatggca
    15721 ttcaaaacag ctaatactgt caccccaaca tctgcaaata ctgcatctcc cattgatgca
    15781 aaaccaaaag cacctaaggc aagtaccaaa atttttatcc caatagcaaa gacaatgttc
    15841 tgtttgacga ttttcaatgt tttctttgct aaatggattg cttctgcaat cttcgatggt
    15901 tgatcattca tgatgactac atctgctgct tcaattgcag catcgcttcc caatccaccc
    15961 attgcgattc ctacatctgc tcttgctagc accggtgcat cgttgattcc atctccgaca
    16021 aatgcggtct ttttattgtt tctttgtaag atttcctcta accgctgcac tttatcttct
    16081 ggaagcaatt cgctatatat cttgtccacg cctacttgct tcccgatatg atcagcaatt
    16141 tttttcgaat cgcccgtcaa catgactgtg ttcttcacac cagccttttt taattgaacc
    16201 aatgcgtcta cggcatcttc tttcaaggta tctgcaataa caagataacc actgtaactt
    16261 tgatcgatcg ctagataaag caatgtaccg atttcttgac ttgaaggaaa agagatttca
    16321 aatctttcca tcaatttgtg attacctgct aatacttgtt tgccatcaac ttcagctaag
    16381 atcccttcac ctgcaaattc ttggatatta gctgttggta aaagcggacc tgcgtagcct
    16441 tctaaaacag attgagcaat cggatgtgta gaaaactgtt ctgcactcgc aacatattgc
    16501 atgaatatct ttggatcgat ttttgtatcg atcgtctgga tcgaaaagtc acctttcgtt
    16561 aatgtgccgg ttttatcgaa taccactgtc tcagtttgtg caagcaactc caagtaattt
    16621 gaacctttta ctaatacacc gattttactt gctcctccga taccgccaaa gaaactaagg
    16681 ggtacggaga tcactaatgc acatggacat gagatcacta aaaatgtcaa tgctcgatag
    16741 atccattcag aaaacgccgc tccagaaaca acaagtggag gtaaaacagc taataaaacg
    16801 gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa attttcagct
    16861 ggtgcttttt tactgctggc attttctact aattctaata atttgctgat agtagaattt
    16921 tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa tccacttaat
    16981 actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc tgacgtatcg
    17041 actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc aggcttcaca
    17101 agtacaaact gaccgatctg cacgtcttca gggttcacgg tcatcaatcc ttccgtcgtc
    17161 tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt tcttgaatga
    17221 ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac tgctacggct
    17281 tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag aaaattctca
    17341 tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc atatcctatc
    17401 agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc aaataatagc
    17461 aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct ctctttttca
    17521 tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc tggttctaat
    17581 tccttcacta ctttttttat ttcttgttca atcgtagcca aatgatcttc ctctgcgtca
    17641 atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt gatcttctga
    17701 actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa tctgtaactt
    17761 ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact aattataaat
    17821 gaacggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc tgcatcggaa
    17881 aatttcttcc atacagatgt tttggcttat tctttctttt tcttttcgat cattgccact
    17941 gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact aagtttgaag
    18001 cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata tcttttttca
    18061 atccctgcaa aatgtccttc ccagatattc gaagctccac tacgctttca aaaatataga
    18121 aagagttctt taatgaaaat tatttcaact ttccataaac agtaacccca tataaataaa
    18181 agacgtaaaa gtcacatgga agaacttata caggagaacg catagagtca ggtgaaatac
    18241 atgtatccca cctgactcta tgctttatct aacctttatt attctctttt atacaatttc
    18301 tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg caaaaggtat
    18361 ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag caattggtag
    18421 gcctgaaacg atcagccaga tgatattaac aatcaatgac atgccagact ggtgataaat
    18481 tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt gcataccgat
    18541 tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc taagaccacc
    18601 gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg tatttctcct
    18661 tctattactg tttttgatta aaactgggca aaaaatgatt ttattcttct ttttttagct
    18721 tccagtatca aatattctgc aaattttttt tggatttctt ccgtattttc ttttgttact
    18781 aatgtgtgtt ggattagttt tcctgatggt cgctgatcca atcgttcttc tgactgttgc
    18841 acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac gagcaatagt
    18901 ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc tggatgatca
    18961 gtaatcagct gattgatttg aggtagatct tcttttactg cccaccgtat ttccatcatt
    19021 tctcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg gcaattgcat
    19081 ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc ccatcttctc
    19141 ttgtatattt cgaaaaacca taaacaatcc ctaaagcatt gaatatacca tctaaaggaa
    19201 ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta gggtctaagt
    19261 ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc atcaagatga
    19321 tgttgcagat tcccactgtt tgaagttcat ttagtaattt tcgataaacg gattcaaatt
    19381 gctgagcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc caggtatcat
    19441 tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct tcttgccagc
    19501 gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat tcaaattcca
    19561 tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct ttacctagat
    19621 cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct atcaccgata aataatatac
    19681 gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt ccttatatta
    19741 tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga caccagccta
    19801 gtcccttcaa acaattgaat gaacgctcat caagtgtcta gttttttatg ctattttttt
    19861 cttaacgtgc cggattcccc ttgattcctt cgttgtttgg tccagagaga tcgatcacac
    19921 ctaagcttcc gtcaatcaga ccactgatga cgccatcaga acatatattt ataggctcat
    19981 ctaagacttc tagtaaatgt ccctttacta atatctcttc tagattgctt gggatagatt
    20041 ggtcataact ttatctggtc cgatgatttc aagggtgaat tgaacttaca tatggattat
    20101 tgcatgctga aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg
    20161 tgctagatgc tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat
    20221 taccttcttt gtaaatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat
    20281 agcaatttct tttgctttct tctcttgtct gctggaaact tcctttatcc cgtattttaa
    20341 aaaaagaact gcgagagttc gcagttcttt tttagaataa acggtgctct ccggccaact
    20401 cctcggtcac aagtcacaat agtcatcaaa catgaaaaac tgttttttga aattactcct
    20461 tgtgactcgg agctgaacgg caggcgcaca acctcttcct taaacggtgt tcaaaagcca
    20521 gtttctcggt attaagccaa atttatgcaa aaatgtggaa agccatttcc acaaaatttt
    20581 tcttaatact cgaaactaaa cagctttttc acaacctctc cactaaacgg tattcaaaag
    20641 cttacatctg cggtaataag tcccgccaat tcaaaaacat gaacagctgt ttttgcttgg
    20701 ctgttctaat tacttgatgc agaacagctt tttcatcacc tcttatggat aaattcggtt
    20761 caacaaacgt gggaatggaa tagcttcacg tacatggtcg ataccagcaa tccaagtgac
    20821 tgttcgttca agacctagac caaaaccaga gtgcggaaca gaaccgtatt tacgtagatc
    20881 aagataccat gaatattctt tttcatccaa accgtgatta cggatttgtt ctagcaagaa
    20941 atcatagtct gttgcccgtt cgcttccgcc aataatttca ccgtaacctt ctggtgcgat
    21001 catatctgca cagatcacca cgtcttctct tgttggatgt ggtttcatgt agaatggctt
    21061 gatggctttt ggatagttta ggataaagac aggctgttcg aatgaattcg caatgaacgt
    21121 ttcatgaggt gaaccaaagt cttcgcccca ttcgatgtca tcaaatccat ttttcttcag
    21181 taattcgatc gcatcatcat aagtaatacg agggaatggc aattttgtat aagtttttaa
    21241 gacttctttg tctcgtccta gtacatctaa tgcatattcg caatgatcta aaacattttg
    21301 tactaagaat gctacatatt gttcctgtac ttctaaactt tcttcttgat gagtgaaagc
    21361 catttctggt tcgatcatcc agaattcgat caaatgacgt cgtgtttttg atttttcagc
    21421 acggaaagtc ggaccgaatg aaaagacttt tccgaaagcc atagcagcag cttccatgta
    21481 taattggcca ctttgagata aataagcttt ttgatcaaag taatttgttt cgaatagatc
    21541 cgtagtgcct tctggtgcag aaccagtcaa gatcggcgga tcaattttga caaaatgatg
    21601 ttgattaaag aattcatacg ttgcacgaat gatttcattt ctgatctgca taatcgcatg
    21661 ttgtcttgaa gaacgcagcc ataaatgacg atgatccatc aagaagtctg ttccgtgttc
    21721 ttttggtgtg atcggataat cgtggctctc gcttaccact tccacatttg taattccgat
    21781 ttcataacca aattttgaac gactgtcttc acggatttct ccagttacta acacggatgt
    21841 ttcttggtgt agatttttag ctgtttggaa aacttcttcc gacacttcgc tttttacaac
    21901 tacaccttgg aaaaaggctg ttccgtctct taattgtaaa aatgcgattt ttccgcttga
    21961 tcgcttattt gcgacccaag cgccgattgt tactgtttct cccacatggt ttttcgaatc
    22021 gatgatttga atttgttcca cgtatgactc tccttttata ttccttgaat ttctttagga
    22081 ttaattttga cttttactct caataaattg cttgattctg cgtaccgctt cttctaacgt
    22141 agcgcgatca gtagcataac tcatgcggac attttctggt gccccaaagc cttcgccagt
    22201 cacaagcgct acccctgttt cttctaaaag atcttctacc cattgtgtga cattttcata
    22261 tttacacatg cgtagcgttt cttttacatt aggaaaaaga taaaaagctc cttgtggctt
    22321 ttcaagagaa acacccggca gttctgccac aagcgaatat aacgtattca ggcgttcttc
    22381 aaaggcgttc cgcatttctt ccactgtatc ttgctcgcct ttcaatgctt caacagctgc
    22441 atattgactg actgctgtcg gattactggt tgactgggaa gcaacagcga tcattccatt
    22501 gatgatcaca ggatttccca ccgcataacc aatgcgccag cctgtcatcg catatgtttt
    22561 agacacgccg ttgatgatga tagtctgatt tttgattgct tctgaaatcg ttgcaattgg
    22621 agtaaactca ttgccattat aaactaaacg accataaata tcatcagcaa ctatcaaaat
    22681 atcatttttt actgcccatt caccgattgc ctgcagttca ttcttgctat aaatcattcc
    22741 agtcggattc gatggagaat tgatgattac ggcttttgtt ttttctgaac gtgtttgttc
    22801 tagctgttca acagtaactt tccagctatt ggattcttcc cctttcacaa agacaggaag
    22861 tccttctgcc agttttactt gctcaccgta actgacccag taaggcacag gaatgatgac
    22921 ttcatcttgc gggtcaagga tcgtttggaa aagggtatac aaagcaaatt tcgctccgtc
    22981 tgttacgact gtttgtgaag gttcatagac aagtccatag tttttcttca gataatcaac
    23041 gattgcctgt cgaagctctg ggattcctgc tgttggtgta taatagctag ctttcccgtt
    23101 gcggattgct tcgattgctg cttcttggat gttttctggt gtcgcaaaat ccggttctcc
    23161 cacagtcaaa ctcagcacat ctctgccttt tgcttttaat gccttggcct ttgccgcagc
    23221 tgccaaagtg acagacggtt ctaatcgttt tgttcttttt gataactcca tcccattcat
    23281 tcctttacct tttaaattcc cttgattgtc ttgacttcat ctccagattc aaagccaatc
    23341 aggtaataat tcagtttccc atttgtttct tttcctacta cttcccacac aggctgatca
    23401 tcatacatcc ctaaagcagc tttttcgatt tggatttcag gatgggccgc agcaattgtc
    23461 tgttttgctt cgttttcagt cagtcccttt gtttgatcca atacttttac tttttcacca
    23521 gatttaggaa tgataacggc gatttctttt cctttatcat ttgtacctgt cagtgaaaag
    23581 tatgtcttct ttcgagtaaa ccaataaaaa tgatcggctt cctcaagatt tgcatatttc
    23641 tttgcgattt ctactgcttc tttcttcgcc tgcaccgccg gacgactgga acgcaagtaa
    23701 aaaatcgagc tgatcacgat gaccgctaat aaaaatacga tcaagccaag taacacgcga
    23761 ttaatcgttt tttcctttgt ctgttttttc ttcaagggtc gtcgctcctt tatcttcatt
    23821 ccttttcatt atagcagatt ttaattatga aaaaagacag ctcctctaac ttccttcttc
    23881 atttttcttt aagaattcat ggatgtcctg atagatttcc tccatagcta gctctttgat
    23941 cggtactttt ttcggtaatg catggatgat ccgtgcacca tattttgcag tgactagtct
    24001 tcgatccaaa ataagcaata cgccacgatc tgtctccgtc cttatcaagc gacctaatgc
    24061 ttggcgtaac ttcaaagctg ctttaggtac ggattcctgg taaaaaggat tgatcccctc
    24121 tgattctaaa taagcgttcc tagctttgac catcgggcga agcggattat cgaagggtaa
    24181 acgtgtgact acaacgatct gcaaagcatc cccaggcaaa tctacacctt cccaaaagct
    24241 gtcagcgcca aacaatacgc ttttttctga caaaatgaag cgtttcaaaa gcttctcccg
    24301 gcttccgcca aacccttgag ctagtatttc tcgtcctttt tctagcatag acagatgcat
    24361 tttctgatag accttctgca aaacatcatg agaagtgaat aacacaagta ttggccgatc
    24421 ttcttgttcg atcagacctg taagtgctgt ctctaaataa tcgatatatg cttgattgct
    24481 gacttgatgg acggcaggtg cctcttttgg cacatagatc cttgcctgtt tctcataatc
    24541 ataaggagag gcaatcactt taagaggtgc tgcttcaatg ccaagccgct tagagagata
    24601 gtgtcgatta gggccgatct tcagcgttcc tccaatataa agaatacgat catatcgagg
    24661 ataccaaaca gtcgacggca acatagccgc ttgaaaatca tggagatgaa aaatcatctg
    24721 ctgcgctgtg ttcccaggaa cgaaccaatg gatcaaatga tctcgccagt cttccagcca
    24781 ttgatccatc agcaagtact gcttgtctaa ttgatcaaat aagctatata aacttgctaa
    24841 atcgatccgc atctggtttg tccagctttc ttggataatc tcaaaagtca gacgcatttg
    24901 tgactgtagc tgctggatct cctgataaaa aagctgcagg tgatcaatgg ctgtcccaga
    24961 ttccagtggc agctgatcca acatctcttt tgtcatcatg acttcttctg gatactgtcc
    25021 gttaggttgc ttgccgctca ctaaactcat taatccttct gccatacaaa tctgtgcttc
    25081 gcatagcgca gaaagttctt ctcgataaat cgataaccag cgtaaggctt catgttcttt
    25141 cggtaaatag ctttgtattc ggtcaaacag ctgatttggt tcttctaatt gctggatttg
    25201 tttatggaaa tagttaatac ttaatcgttt gtcatttact ttttcagcga tttccggcaa
    25261 atgatgtact tcatcgatca acaaaaatgg actagctgga agcaatggtt cttttcgctg
    25321 tgtttcttgt attaaaaacg catgattgac aatcaaaaaa ttactttgcc tgattttctt
    25381 ttgcaagaaa cgaacaaagt cttgttcgta aaatgactga tttcttgcta aaaatccaat
    25441 acctctgtgc gcgatatctg caaagaatga atggttcaaa tttgttaaat gtaattcatc
    25501 caaatcaccg gtcttcgttt gcgtcagcca aactaagacc gccatttgat acaaagcata
    25561 ttgtttttgt tctggcggtt ccacaagtgt gcctttaaaa cgctgtaaat caatatagtg
    25621 gcgatagctt ttgataatta caggttgtat gggctgatct agcaattgat tcagcaatgg
    25681 aatgtctttt tctatgatct gctgctgcaa aactagcgaa acggtactga tgacagcggg
    25741 tttttcaggt gtagccaaat aactcaatgg cagcagataa ccgattgttt tacccattcc
    25801 tgtagctgct tcgatcatca aatcttttga ttcatctttt gtgaaatggt cgtagaccgc
    25861 attcatcagt ctgttctgtt cttttcgata catcagtgtt ttaccaaaca ttttttcttt
    25921 tgcttgtttt tttcttggat atgtccgctc gccataatgg acggatgtga atagttccac
    25981 ttcttttttc tgcaaagcta gcccatcgac gatttctaat gagggatcaa gtggctctgg
    26041 tttttctttc atttcttcaa tcacatgata aatgaaacga ctcgtatcca tacctgtgtg
    26101 tctacttaat cgcgcaatct gctctaacgt gatgattggc aattttctca tccgttcttc
    26161 gatcagcaaa agaagttgac ccgtcacttc ggcatcacta tctgcctgat gaggattctc
    26221 atgaataaat ccaaggcttt ctgacaaatc agctaagcga aagcttggtt ccgtcggcaa
    26281 gaaaatttgt gcaagttcta ctgtatcgat gcctggtatc ttcaaactag gaagtccgca
    26341 tcgcatgagt tcatggttca aaaaattata atcaaaataa atattatgcg cgacaaatat
    26401 ggtgtctgcc aaaagattgt aaatcgtttg ggcgacatct tcgaaataag gcgctttttg
    26461 gacgcgttga ttcgtgatcc ctgttaaatg ctggatttgt tttgaaatct tccgccctgg
    26521 atgaatatcg atcgaaaaac gtgaagtgat ccgacctgat tcaatcatga cgcagccaaa
    26581 ttggatgatc cgatcttctt ttggattcgt cccagtcgtt tcgatatcaa cgactgcata
    26641 agttcttggt ttcaaaatat cttcctcttc tcctgcctct aatgttcttt tgaaattata
    26701 ctacaaagtg cagaaaaata ctctcatcgc tttttaaaga aaatagacaa aaacgcccag
    26761 acacgcaaat gtctgggcgt ttatttttta gtataggtta attaagcttt agtaacgttt
    26821 gttgcttgag gtccgcgttg gccttcttca acatcgaaag ttactgcttg gccttcttct
    26881 aaagttttga atccgtcgcc ttggatagct gagaaatgaa cgaatacatc gtttccgttt
    26941 tctgcagtga tgaatccaaa tcctttttct gagttaaacc attttactgt acctgtttcc
    27001 ataataaaaa tcctccttgt gtatcaacac atattttgca attacttgaa tggtgtatat
    27061 ctggaagatg tttatttgaa acaagctacc acagattacc gaacaaaaac tactcctata
    27121 gtatatcata ccgtttacaa aaaagctaga gtttttcgac tttttttatt aaaaaaagtt
    27181 ttctcagcga ttacataaag tgaaaaaccg aacttttttt aaaataaacg acatattgtt
    27241 cagttggtgt ttttttgctc tgatgttctt ctttaaacta aaaaaattat tctttctgac
    27301 tacttatcgg ttctgtttca acggtcgagg ctggggaaga ttctttagta ggtctgatgt
    27361 tctcatggta tttctgatag cctatgacag ttaccagaag aacaaggcaa accaatacga
    27421 tcaacgagat tagtttttct tttttgctca tatctatacc tcataaataa caggatgatg
    27481 tccatcatat ttctgagtat gagtaaaagc aaaagctgca gctgtagaat gtaagaaatg
    27541 ccagcaactt tcataatcac aggtcggaca gtaccaaaag taaccaaatc ttggctgcaa
    27601 agaccgttct tctgtttcag gaatcgcaga atcaagatct gttgtctgta cataaacatt
    27661 tttgtacaga cgtgtgcctg taataggaga ccgctgtgtt tcttcagcaa aaactggctg
    27721 acttgttaaa cttagggata gaaacagcaa acaccataac agtctttttt tcataaagac
    27781 ctccttatcg ataagttttt cccaattaaa atcatatctc aagaacgccc gttattcaca
    27841 ttttgttcat aaatgatgtt ttttgtatca taatcgtcaa aaagaggtcg tgaccacgtc
    27901 acaacctctt tttt
//
LOCUS       DASHFF010000030        27641 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.030, whole genome shotgun sequence.
ACCESSION   DASHFF010000030 DASHFF010000000
VERSION     DASHFF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.030"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(18..293)
                     /locus_tag="U2I75_001499"
     CDS             complement(18..293)
                     /locus_tag="U2I75_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696643.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            587..1402
                     /gene="thiD"
                     /locus_tag="U2I75_001500"
     CDS             587..1402
                     /gene="thiD"
                     /locus_tag="U2I75_001500"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN1696644.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1607..3058)
                     /gene="cls"
                     /locus_tag="U2I75_001501"
     CDS             complement(1607..3058)
                     /gene="cls"
                     /locus_tag="U2I75_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1696645.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3399..6065
                     /locus_tag="U2I75_001502"
     CDS             3399..6065
                     /locus_tag="U2I75_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1696646.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6429..7181
                     /locus_tag="U2I75_001503"
     CDS             6429..7181
                     /locus_tag="U2I75_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1696647.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7203..8357)
                     /locus_tag="U2I75_001504"
     CDS             complement(7203..8357)
                     /locus_tag="U2I75_001504"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN1696648.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8542..10983
                     /locus_tag="U2I75_001505"
     CDS             8542..10983
                     /locus_tag="U2I75_001505"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN1696649.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11313..12017)
                     /locus_tag="U2I75_001506"
     CDS             complement(11313..12017)
                     /locus_tag="U2I75_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696650.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(12204..12449)
                     /locus_tag="U2I75_001507"
     CDS             complement(12204..12449)
                     /locus_tag="U2I75_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696651.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12466..13317)
                     /locus_tag="U2I75_001508"
     CDS             complement(12466..13317)
                     /locus_tag="U2I75_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1696652.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13483..14487
                     /locus_tag="U2I75_001509"
     CDS             13483..14487
                     /locus_tag="U2I75_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN1696653.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14728..15084)
                     /locus_tag="U2I75_001510"
     CDS             complement(14728..15084)
                     /locus_tag="U2I75_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1696654.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15452..16639
                     /gene="tuf"
                     /locus_tag="U2I75_001511"
     CDS             15452..16639
                     /gene="tuf"
                     /locus_tag="U2I75_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1696655.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(16827..19418)
                     /gene="mprF"
                     /locus_tag="U2I75_001512"
     CDS             complement(16827..19418)
                     /gene="mprF"
                     /locus_tag="U2I75_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1696656.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19444..20205)
                     /locus_tag="U2I75_001513"
     CDS             complement(19444..20205)
                     /locus_tag="U2I75_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1696657.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            20372..21004
                     /locus_tag="U2I75_001514"
     CDS             20372..21004
                     /locus_tag="U2I75_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN1696658.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            21063..21923
                     /locus_tag="U2I75_001515"
     CDS             21063..21923
                     /locus_tag="U2I75_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN1696659.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            22108..24423
                     /locus_tag="U2I75_001516"
     CDS             22108..24423
                     /locus_tag="U2I75_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1696660.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24605..26446
                     /locus_tag="U2I75_001517"
     CDS             24605..26446
                     /locus_tag="U2I75_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN1696661.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26470..27579
                     /gene="rpoD"
                     /locus_tag="U2I75_001518"
     CDS             26470..27579
                     /gene="rpoD"
                     /locus_tag="U2I75_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN1696662.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         8943 a         4886 c         5763 g         8049 t
ORIGIN      
        1 taaagaacag ttagaagtta aaattgctga ttcacataga gtttatgact tcttggaaat
       61 tctttttcaa tcagtttttt catctgttta tgaaattctt ttttttcagt aaacataaag
      121 gtttgggtat cgtaaccttc ttcatgtagg aaatagatta ttcctttttc tgtgttttgc
      181 gtataagctt ttatactcct gtcttttttg aaagaacaga tcgtatagcc atctatttgg
      241 tctggtagtc gtttgatttt cttcaaaatg acttttttat cctcaagttt catttttttc
      301 acctgacttt atttttttca tatggaaatc atagcgcctt tggttatatg aaaacaagaa
      361 ggatactgaa atatatataa atcaaatagt ggttatttat tattagaata gcgaatacaa
      421 acaaatgaca actattttct gatttattac aaatgttttt tcaaataaat ttgattacca
      481 agaaaaggta gtggtatatt tactgaggga tactttgaaa aacatatgat agaaacagta
      541 acagcattat gaaaaatctt tttataaaaa tggaggaatc gatttgatgg aagatcagta
      601 tgtacaagca atgacgatcg caggatcaga tagcgatgga agcgcaggga tgcaagcaga
      661 tatgcataca ttctttacaa gaaatgttta cggtgtttct gtcatgaccg catgtgttgc
      721 aggtaattct tacggaatcg gagcaagtgt gacattgcca acagatttta tcgacaaaga
      781 atttgaactg attgcgaagg actttcaaat acgtgcagct aaaacaggta tgctagcaga
      841 ttcaaagttg attgagacag ttgtaaaaaa ttaccaaaaa tatgattttg gaccattagt
      901 cgtggatccc gtaatcgtaa cgaaacatgg aaatctttta ctagaagaat ctgctttgca
      961 aagcttgaaa gaaaagcttg tccctttggc cgaagtactg acacctaatt tttatgaagc
     1021 agaaaagctg actggacgga cattagcagc tgaagaagat tttgtgcaag cagcaaaaga
     1081 actgcaagat atgggtgcaa aaaacgttat gatcaaagga catcatgcta aagatgcgca
     1141 gcaagaaaca gtcaatgact acgtattgct cgaatcagga aaatcatttt ggttaaatgc
     1201 accattttat cctaccgacc ggatcaacgg aactggcgat tcgctatctg catgtattgc
     1261 agctgagtta gcgaaaggaa aaacagttga acaagcgatc cgattggcaa aagactatgt
     1321 aaaccgtgca attcgtgaag aaataaatgt agggcacaaa tatggaccca tcaatcattg
     1381 ggcagctggt gaattagagt aaaagaaaga ggtcgtgagc ctgccgtcca gctccgagtc
     1441 acaaggaaca agttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg
     1501 aggagttggc aggagaacac cgtttgttcg gaagaaggga ctgtgacaag atttttgtca
     1561 cagtcccttt aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag
     1621 aaaataatcg agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt
     1681 cagttgtcat ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta
     1741 aaaactcagg atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat
     1801 cttgatttgc tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga
     1861 ggaagccgtt ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat
     1921 attgtgttgc tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat
     1981 ctacaccaga ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt
     2041 aaggtgtctg tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct
     2101 tgatttgttc cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa
     2161 gcttagattc atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt
     2221 tttcttcagg taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag
     2281 cggcaccgaa aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta
     2341 catattgatt ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga
     2401 tttttcggtg atcatggtaa ttcaatcgga aacgaagtaa tgccttttgt gaggtaacaa
     2461 atgtatggac atgtccacca tttttgatta gacgattgaa atcttttgct tttgtaccat
     2521 gagagccaaa tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta
     2581 aaacatgtaa gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga
     2641 tcgaatgttt ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg
     2701 tgagaagagt gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt
     2761 cgacaaattg ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc
     2821 ctctaaaaga ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt
     2881 tcttatccgt taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg
     2941 caaacaatac taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag
     3001 ccaccaaagc aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa
     3061 aaatgttcct ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc
     3121 aaggtgaata tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt
     3181 gctttttgta ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta
     3241 accggcaacg tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt
     3301 gtatgaaatg gtgagaccta agacaaatgc gtatttgtct taggtctctt ttttttcaga
     3361 caggactagg gccaagatca aaaaatgggg gatgaaattt ggaaaacaag gaacaagcac
     3421 aacaagcggt ctataaagaa acgatcgact cgttgatgca gcaaacgcag agctcaaccg
     3481 atggattgag caccacgcaa gcgaaggaaa aattagaaag agatggcttg aaccagctaa
     3541 aagaaaagcc gaagaagcca acttggaaat tgttcttaga aactttcaaa gatgcaatgg
     3601 ttatcgtatt gctgattgtc gcactgattc agatggtcat gggtgactac atcgaattct
     3661 tagttatttt tgcagtattg atgttgaatt ctataatcag tgttgtccaa acaaaaaaag
     3721 cagaaggttc tttagaagcg ttgaaaaacc tttcagcgcc cgatgctcga gtgattcgtg
     3781 atggtaagga aaaaacgatt ccggcaaacg aacttgtggt aggagatatc gtgttgttag
     3841 acgcaggcga ttatgtacct gctgatggac gtttactaga tgcgggatca ttaaaagtag
     3901 atgaaggtat gctgacagga gagtcgactt ctgcagaaaa agaagtaacg gatatcacaa
     3961 gtacggtacc aatcggagat cgtacaaata tggtattcag tggaacgatc gtaacttacg
     4021 gacgcggtaa attcttagtg acttcaactg ggaataatac ggagatcgga caagttgctg
     4081 gccttttgga atcaacaact gagcaagtca ctccgttaca acgaggtctg gataaattca
     4141 gtaaaaaact aagtctggct attttggcat tgtctttatt gattttaggt atccaactgt
     4201 ttcgtattta tcttggagaa ggtacaggag atatgactgc agatatcgtc agcgcagtga
     4261 tgtttgccgt agccgtagcc gtagctgcta ttccagaagc attacagtct atcgtaacaa
     4321 tcgtgttatc tttgggaacg aataaaatgg caaaacgtca tgccattatt cggaagcttc
     4381 cagcagttga aacacttggt tcgacaagta tcatctgtac agataagact ggtacattga
     4441 cacaaaacaa aatgacagtt gttgactact ttttaggtaa tggcgatact ggggatttca
     4501 caaatgaccc ttcaaaatgg acagctgacg aacgtcgctt gatcgaaatc agtgttttag
     4561 cgaatgatgc atcgatcagt gaagacggta ctaaattggg cgaccctacg gaagtagcgt
     4621 ttatcgactt tagtgaaaaa ttaaaccagc cttatcagga aatccgaaac aactacccaa
     4681 gacaagcaga acttcctttc gattctgatc gaaaattaat gtctactgga cacacgatcg
     4741 ataacgaaaa ttacttgttt gtcaaaggcg gaccagatat cgtcttccaa cgcagtaaaa
     4801 aagtattgct aaatggtgaa gtcgttccga tgacggatga attgatgaaa aaattccaag
     4861 agcaaaatga agctttttca acacgcgcat tacgtgtact agcttttgct taccgtccac
     4921 ttgataattt tgatttatca tttgaagatg aagacgattt gatccttgtt ggtttgcttg
     4981 ctatgatcga tcctccacgt gctgaagtga ctcaggcggt aacagaagca agaagtgcag
     5041 gaatcaaaac aatcatgatt actggtgacc acaaaacaac agctgttgct atcgcaaaac
     5101 aaatcgggat ttttgaagaa ggaaacctgg ctttgaccgg gacggaacta gatgaattga
     5161 cagaagatca actaaacgaa gttttagaaa aagtcactgt ttatgcacgt gtttcacctg
     5221 agaataaaat tcgtatcgtt cgtgcttggc aaaataaagg tcatatctca gctatgacag
     5281 gtgatggtgt aaatgacgca ccagcattga aacaagcaga catcggaatt gctatgggaa
     5341 cgggtacaga tgtggcaaaa gatgcagcag cgatggtatt gacggatgat aactttgctt
     5401 ctattgtcag cgcagtagaa gtaggacgta atgtgtttga taatatcaag aaagctattt
     5461 cttatctatt tgcaggaaat ttaggtgcga tcattgcgat catctttgcc ttagttgctg
     5521 actggtccaa tccatttaca gcattacaac tgttgttcat caatttagtc aatgattcac
     5581 tgccagcaat tgcattaggt acagaaaaag ctgaacctgc tacgatgaac cgcccgccaa
     5641 gagatccaaa ttcaggaatc tttacggaca aaacattgat ttctgtaacg tatcgtggtg
     5701 tgctgattgg gattgctgtt atctttgctc aatggcttgg catgcagcat tcacctgaat
     5761 tcggtgtagc catgtcattt tcaacattga tttggtgtcg gacattgcaa acattcccag
     5821 cacgttcaaa cacacaaaca gctatccaag cagggttctt ctctaacaga agtgtcttac
     5881 tagctgtagt tgtatgtagc gcgctttact gcttaacaat gatcccagga ctgaaagaag
     5941 tcttctctat cccatcagca tttggattga cagaagtctt tacatgtatg ggaatcgcac
     6001 ttgtgacaat tgttttgatg gaaatcacta aattgatcat tgtacgagta caaggaaata
     6061 aataaaacga atcaacgagg ttgtgagcct gccgtttaat tctgagtcac aaggtgcaat
     6121 ttcaacaaac agcttctcat gtttgttgaa tattgtgact tgtgaccgaa gagttggcag
     6181 gagaacaccg tttaaggaag aagttgtaaa atcagcattt tgagctaata ctgagggtca
     6241 tctgattgga cactgtttat tcggaaagag ggactttgac acgactttgg tcacagtccc
     6301 tttttttatt tatgatagac ggaaagccaa tcattttatt attcatcaaa agagatagaa
     6361 tagataaaag aggagaataa tttattccga aaagtgaaaa catataacaa aaaattagag
     6421 gaaatcaaat gaatgaaaaa gaatgggatg aatttgctga agaatattat gaaatccagc
     6481 aagaatcgca agtaactatt gctgaagatg taaaaaatta tctgaaaacg gagaaagtcc
     6541 tgcctgctga aacagcagca gacgttgctg gaggatctgg cagatttctt tccttagcaa
     6601 aagaaattaa tcgctatgat ttgatcgatt tttcttcagg aatgctagaa aatgcaaaaa
     6661 aagaagcaga aaaaaatgag ttgccaaatg tttcctttat caaacagtcg ttagaagaat
     6721 ttatccaatc aaatgagaca tatgaaatgg tttttacagc agcaaatcca gcattggtac
     6781 atcttattca attagaaaaa ttgatgaaaa agtcaagcaa atggtgcgtg ataatcaggg
     6841 tagtggaatc aatcgatgat gttttttatc ctatcgaaaa gcaactagga atgatagaag
     6901 aagatcctaa tacatcgcca gttgtaatgg atcaatttga aacatactta attgatgaag
     6961 gattcaaagt gaagaaaaaa gattttactt attttacaca ggaatcaatt actgaagaat
     7021 tgatgaagct atactatgac gcgtacgcaa aagatcctgt tgtgaccagg tacattcgca
     7081 aactattcag tgaaaaacga gaaatcctta gtacgactaa gctgacctat agactgctcc
     7141 tttggtccgc agataaaaaa agaacctttc acgactcgtg aagggttctt tttatgattt
     7201 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
     7261 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
     7321 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
     7381 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
     7441 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
     7501 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
     7561 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
     7621 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
     7681 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
     7741 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
     7801 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
     7861 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
     7921 gccaagccgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
     7981 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
     8041 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
     8101 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
     8161 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     8221 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     8281 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     8341 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     8401 gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt
     8461 aatcttagaa aaaaacaact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
     8521 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
     8581 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
     8641 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattc
     8701 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
     8761 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
     8821 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
     8881 ggaacagaga gtatgtctca agcacctatg ctgaaaccgt atcagtcaga aacaaatgaa
     8941 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
     9001 catatgggat taaccgcaga gaaggttgca acacaatttt ctgtgagcag agaagaacag
     9061 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
     9121 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
     9181 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
     9241 ggaactgtaa cagcaggaaa tgcttcgcca ttgaatgacg gtgcctctgt ggtgatcctt
     9301 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
     9361 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
     9421 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     9481 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     9541 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     9601 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     9661 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     9721 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     9781 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     9841 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatgggcatc
     9901 gcgcagaatt ttcaaatcaa tggtaagaaa aaatggattc cgatggcaac agaggaacca
     9961 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
    10021 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
    10081 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
    10141 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
    10201 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
    10261 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
    10321 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
    10381 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
    10441 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
    10501 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
    10561 agcgcttcga ttcacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
    10621 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
    10681 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
    10741 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
    10801 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
    10861 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
    10921 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
    10981 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcaa
    11041 atcttgcttt tttctctatc agaaaatatg tttattatta gaatttttta tagttaaggt
    11101 taggcatctt aagaaatcag gcaactccaa gatatcttca ggtagaagag aagtcgccta
    11161 acattgtagg atcataggtg atttgttgga caggtcaagc tgatatttga gcctgatgtt
    11221 agaaggaatt tctttttact tgctgtagaa attattatat gactaaaaag gctgggattt
    11281 taaatcccag ccttttatga caaatagaat ttttatcgat cagtaatcgc tgtcgctgca
    11341 aattgttcga tcaaaggaat caaagtcaat ttgactttgt atttccgttc gagtgctttt
    11401 tcgataacga actcattttc agtagtcata gaaatagttg ctaatttacg aggttcatca
    11461 gcattttttt cattaataac tacttcatgt tcattttgtt ctgtgtaaaa tttaggatac
    11521 tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg cattcgtatc agttgagcgt
    11581 gtattttcag ttgtgtccgc ttttggtttt tccgtttttt cttctggcac actgactgtt
    11641 tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt catcgttttt caatcgttga
    11701 tcattccagt aagacacgat ttcaataaga agattgtcac ggaatcgttc gaaaatcccg
    11761 ttttctcctg aacgaattct ctttaataca gtattgaagt tgtcatcgat tggcacgttg
    11821 atccgtgtat cttcatttgt tttcccagct aatccgatcg ttaagatttg acggtttttg
    11881 ggatcaagca tgacttgtaa cggcaaacca tagcgtcgca agctctcttc ttctagagaa
    11941 cggaataatt gtcctcttac accatccaat gaaggacgga attgatttaa taatttttgt
    12001 ttctgttttt tcttcatatg ttctctcctt cgctcatacc ctttcgatta taagaaaaaa
    12061 acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta tacgcaaatt acaacagaaa
    12121 aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa atggaacgga ttatgtatat
    12181 atacataatc cgttccattc tttctatttt ttcggtatca aggcaaaaac aggtagttca
    12241 ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt
    12301 tttttcagtt cttcgatgat ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt
    12361 ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag
    12421 agagaattca agcgttcgat tggatccata aaatcacctt tcgacttaaa attgggtagt
    12481 atctgggtct tttgctttgc cgactacacc atctaactga tcgatttgtt tcatttcttc
    12541 ttctgttaat tcaaaatcaa ataattcagt attttctttg attctgcttg gtgtcactga
    12601 cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt
    12661 cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca tcgaagattt taccagtgcc
    12721 taatggacta tatgcttcga ggatcatatc atgttttttt gcatactcaa ctacagttgg
    12781 ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc ggcataattt ttgctgtttt
    12841 tgctagtgta tctagatggt ggggtaagaa attgctcaca ccgattgctt tgattttacc
    12901 tgcttcatac agttcttcaa atgctcgcca tgtatctgca tttgcttgtt cccaattatc
    12961 acgaaaagca acagggtttg gccagtggat caaaaacaga tctaaataat ctgtttggag
    13021 cttcttcaat gattcttcaa aagaagacat cacaagttca tagctgtggt ttgcattcca
    13081 taattttgtt gtcaagaata tctcttctct aggaattcca ctttccttaa tcgcttggcc
    13141 gacactttct tcattcttgt atccttgggc agtatcgata tggcgataac ctgcagccag
    13201 cgcttcttta acagaggaaa cagcgacatc cccatcaggt gtttgccatg tgccgaatcc
    13261 tactaccggg atttcgtaac catttaatag acgataagtg cttgttaatg aattcatact
    13321 ttcagctcct tttttattcg ttcaactata agtgtagcgc atttttcgaa aaaaagaatc
    13381 cattttgttt ctactcttga aatttttgtt taaaagaaaa tgattttgat tctggtattt
    13441 ttgatttttt ttccgtaaac taagacagaa gggaatgaga aaatggatat ctatttaaaa
    13501 aaagcaattc tgcatattat tgacagagaa actggttctc cagtcttttc acaaaaagag
    13561 ctggatctga caaaagaata tattcgtgat tttcttcaaa agaagatcca aaaaatctct
    13621 tctgctcaaa caaaaactgg acaactagca gaagaaagca tgatcgttga atcacttaaa
    13681 atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc
    13741 taccaagaaa gtgaagaagc acctagtgca gatgtatttg ttgttttgta tgaattagat
    13801 acagtgatgt acttatcttt attgaaagtc aattatcgtg aggcttacac tcactttgtt
    13861 gaagcagacg aagcagggat cgacaaccgc ttgattttga atcgagcaat tctaggggcg
    13921 aaaacacaaa aagcagatga agcattcgct gtgaatttga ctgattggac ctacgaatta
    13981 attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt atttttctgg aagagtgatc
    14041 gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag tgatccaaaa agtagccaag
    14101 catcttggaa aaaaatttga gacagaagaa tttgatatca tggctgattt gaaagaagct
    14161 gtctatgata cgatagaaga aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc
    14221 ttcaaggaaa acatcacggc aaaaatggct tttaaagaag aagtacaaga gcaaggattt
    14281 gtcccagaag cacctcctgt aaaagaagta caagaaattt ctgagaaaaa atttggaaaa
    14341 caaaaattca agctatcaaa cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac
    14401 ccagatttga ttgaatttgt gaataatccg gatggtacga tatccgtgat gatcaaaaat
    14461 gtggatgaag tattgaatcg tttataaaaa tgactagaac attaggtgtt caagtaccag
    14521 tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa
    14581 gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
    14641 gaagcagaag caactccttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
    14701 tatctaataa aattttaaga ccatttacta attatcgatt ttttctgccg catttttagg
    14761 cacttcagaa gcctttattt cttcccagct taatacacct ttacgaggat tgactttgag
    14821 tttaagatac gcattcatct ttaatggatg ttctctttct tcgcgaagcg tcacctgttt
    14881 tttttcacca tctttattat aagccgcttg cttgtaggta taagtcgtga atttctctcc
    14941 gttatctgct tgggtagctg acatttcgcc attcgtcgtg atttttgtat aataagcttc
    15001 gccgccgtaa aaataatgat aagctgcaaa acttccgccg agaaatatgc ctaaaacgac
    15061 aagtacgcca attaattttt tcatgtactt cacctctttc cttattatta acgaaataga
    15121 agatttcgtc tattgaaagt tgttggcaac agctgaattt taaacttttc ctcattttgt
    15181 tcttattgct tcaaaagaaa gttaaggact atcttgaagt ttttctcaaa aaacacattt
    15241 ttttatttat aaaaggtact tttttcgcgt ataagacaag cgagtgctct tagttgactt
    15301 tgaccaatat tgacgtataa taagcttagt tcgaatctta caaaaagtaa gtcggacgta
    15361 ttctttttga tttagtcaaa aaaagactat aataaaaagg tgagagctga aatttttcag
    15421 tgatctatat tatttaggag gtttgtaaat tatggcaaaa gaacattatg acagaagtaa
    15481 accacacgtt aacattggta cgattggaca cgttgaccat gggaaaacta ctttaactgc
    15541 tgcgatcact acagtattag gcaaaaaagg cctagcaaat cctcaagact atgctagtat
    15601 tgatgcagct ccagaagaac gtgaacgtgg gattacgatc aataccgcac acgtagaata
    15661 tgaaacagaa aaacgccact acgcgcatat tgatgcacca ggacacgcgg actatgtgaa
    15721 aaacatgatt acaggggcag cacaaatgga tggtgcaatc ttagttgttt ctgcaactga
    15781 cggtccgatg cctcaaacac gtgaacacat tttattgtca cgccaagttg gtgtaaaata
    15841 cctgattgtt ttcttgaaca aagttgattt agtcgatgat gaagaattga tcgatttggt
    15901 agaaatggaa gttcgcgagt tattgagcga atatggtttc ccaggcgatg acactcctgt
    15961 gatcaaaggt tccgcattaa aagcattgca aggcgatcca gatgctgaag ctgctattat
    16021 ggaattgatg gatacagtag atgaatatat cccaacacca gaacgtgata cagataaacc
    16081 attactattg ccagtggaag acgtcttctc aattacaggt cgaggaactg ttgcctcagg
    16141 tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta gagatcgtag ggatcaaacc
    16201 tgaaacacaa aaagcagttg taacaggtgt agaaatgttc cgtaaaacgt tagattacgg
    16261 ggaagctggg gataacgtag gcgtgttgtt acgggggatc caacgtgacg atatcgaacg
    16321 tggacaagtg cttgctaaac caggttccat tactccacat acaaaattca aagcagaagt
    16381 gtacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcaaca actatcgtcc
    16441 acaattctac ttccgcacaa ctgatgttac aggaacaatc acattgccag aagatacaga
    16501 aatggtcatg cctggtgaca acgtgacgat tgatgttgac ttgatccatc caatcgctgt
    16561 tgaaaacgga acgacattct caattcgtga aggcggacgg acagtgggtt caggtatcgt
    16621 tacagagatc gaagcataag aaacgcgact cgaatatttt ggtaatgaaa aatgtttaaa
    16681 ataaaagagc ggtccaaaca ggatttctgt ttggaccgct ctttgttctt caattgtata
    16741 agaagagatt gtagaagcat gaactttcgt tttacttcag tctcttttat tcatcatgag
    16801 agtgttcatc tatcggtatg cagtttttaa tactttcttc gtatccattt tttcaaacca
    16861 cttattctac cttgtgtctg ctcaactggc gcattatcaa taatcagcaa agcaatcata
    16921 acatatgcaa tccaactatc tctagaatat agagtataac gtgagaccca gtttgtagcg
    16981 tacttgcttt tatattctct taatccatgg aaagaataaa aatgagaacc aaactcgtaa
    17041 accaagtatg caatcctttc ctgtaaaaaa ctctttctgg aagttccgac atttgaaaga
    17101 ggggccatcc ctaaattgaa ccattggata tgttcttctt tcatataggc aaacaaatgc
    17161 aagaacaaga aatccatact gccagaaggt gcttttttag gatcatatcg catcagatca
    17221 atcgttccta ctttattgtt cgtgtaagta ggcatgatcg ttgcaaaagc gacaatttct
    17281 tcttgggtat ttttgacaac tgcaataggc gctctttgta gataatcttc agagaaaaag
    17341 cctaaagaaa accctttttc ctttcgtgaa cccaaccaat tatcagagat atttttgaaa
    17401 gtactcattt gctcagtaga aaatggtggt tgtaagacat caaatgtaaa tccttctcgt
    17461 tcgatccgat tcaagacagc acgagtgccc ttcatttttt tccctgaagt tgtaaaacta
    17521 ttcaggtcta cataagcttc ttcccccatc ttaatgaaat cgtagccgaa ttcatgcaaa
    17581 atcataacaa tctcttcgct tgtttcgtag aaaactggta aatagcatag tcgatctgtt
    17641 tcttcgataa aagcttcgat tgcttctgga aaatcttctt tcttaccaga aggatcaccc
    17701 attacaatac atttattatt gaagcaagcg aattggagaa gaacagttgg ttcaccgtct
    17761 ttttcatagg caaacatccg cttatctttt aagaagatca attgactatc ggagtttcct
    17821 ccataagttg taaggatctt cagggccttt tcttcattaa aggcttcgcc aatctgtttc
    17881 ttttctcctt gaaggaaatg aacaaataga acgatcatga aactaacagc gatgatggcc
    17941 aacaatcccg aaaaccaaat cttttcagaa ggaaataaga aaaaggaaat aaaatggtga
    18001 cgccgatgag ggaaatccgg cagattgtat accccaataa cgatgtaaag caaggacagt
    18061 gcaccaataa tgattccatc gatcgtacgc cactcccatg aatagatcaa ttgttcccga
    18121 aataattcgt ttttagaagc aagaatgatc agaagcagaa tagacaagaa aaccaccggt
    18181 gtgaaactaa aatcacttaa taaaacataa agtaaagcta gaacaatcaa gaagattgta
    18241 ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc ccataatgat taaaagaaat
    18301 cccaatagta tactaggaaa ttggataata aaatgaaatt tcaatgggtt caaactatga
    18361 agccagcgaa actctgtaaa agcttgcggg atagtggcag aaagtactag catgattcca
    18421 gaaaagtata ataagacaac cacgatttta tgcgcaattt ctgttgctaa ttgtttaggg
    18481 ataccagaat aacgctgatc aaaagtggaa ccgatatttt tgaagaaaaa gacgattcca
    18541 atcaaaaacg gaatgatgta gtagaacagg cgatagagca agatccacac aacgactatt
    18601 tctcttggca caccaatagc ggacaacccg ataatcatca ttacatcgaa actcccaatt
    18661 tctcctggaa tcatagacac gattcctatc actgaagcag caatgaataa cggaattgtt
    18721 tgccacagat caatccggat ttccattaaa taaccaacag aaaggaaact aaacaggaca
    18781 ccggtccatt ccaacaatga aactagcaat agctgtcctt gagttttaag tgaggtacca
    18841 ccaaggaatc ctttcttttt gaaagtagta aataaaaaga cgacaggaaa atatagaccg
    18901 ccgccgatca accatatcca atattgcttc aaaaaaggag caactggcgt gaagactaca
    18961 agtaaaaaag aaagcagact gtaaatagaa agtccagcca tcaaaaacaa gaaaactttc
    19021 gataatgctt ggatcacgtt tctgctctct ttcttttgtc catatagttc agagcgaagc
    19081 cccacactaa caaacccgcc gaaaccggca atattgttga ttgtattgat cagccagctt
    19141 gtctcaaaca agtaacgagg attctgcttt tgttcaagca tccgattcaa aataatatca
    19201 taaccaatca taggtaacac agagacaaga ccaatcaaca gcataagtcc gcttttccat
    19261 acaggtatag tggcaaacgt ttctcctaat tgctggatag atagagtttt accgatggac
    19321 atcaattcac cgacaataat gacaatgaca gaaatcaaaa agattgtttt gaacagtcct
    19381 aggtggtttt tcagccattg tatggtattt tttaacaaat aaatcacctt ctattcctaa
    19441 tagttatgtt tcagaaagaa attgaagaac ttctcgattg aagcgttcag gatcttttct
    19501 agctaattca tgtccttgtt ctttcacaag cacaaaggaa gcttttggta tagttttagc
    19561 tatatacaaa gaatgtttta gcttgattac atcttttttt cccacgatga tcaaagtggg
    19621 ggagttgatt ttttttaaat cattttctgt aagcccgatg tcatgcagca aaagtttgat
    19681 aactaaaaga tttttgcgaa gacttgggcg aaataaagag agtaaccata cccatgcata
    19741 atgaatatta ctgatgactc tggcagaaaa acgaacgcct ttaacaagcg tgtttccaga
    19801 atttaaaatc aatcgatgaa cttttttagg gaagctgctg gcaaatacca gtgctagatt
    19861 tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt ttttccagca acatgatcgt
    19921 attcagatcc tcggccatta actgaaaatt caacatagat gcttcgtttg tcgaacgacc
    19981 atgtcctcgg ctatctacta gatacacggt atagtagcgt tcgagtacag ggacctgttc
    20041 tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa aagagaggga agccttgacc
    20101 gctcttttca tagtaaattt cgctcccatc tggcattttt gcatatttct tttctttttt
    20161 cacataatca cctactgtgt aaagtataac gattatatca gtcattgggc aattgttttt
    20221 actgaaaaac aacgatcatt ttcgtaagat gacaaaaaat acaggaaaat tcattttttc
    20281 ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa gataaagaaa tagtaagata
    20341 aaaaaagatg tcaaaataac taggtgatga aatgaagcga aaacgattat ctattatccg
    20401 tattcttttt tacatgatca ttctgacggt tattggtgcg gtgttgtttt taggaggaag
    20461 aacttatcgt tcggtaaaac gagtggaaag ctatcaaata gaaataacaa cagctgcaaa
    20521 aaattatcat atggaaaact acgagaattt gatcaaggcg ataatattga ccgaatcaaa
    20581 aggaaaaggg acagatctca tgcggagctc ggaaagtctg catggagaag ctgagatcat
    20641 tgataatcca gctgacagta ttgatcaagg tgtcaaatat ttggcggaaa tgatagtaga
    20701 agcaaagaaa caaggatgcg atttatggac agctgtccaa gcatataatt ttgggaaaga
    20761 ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca gtagaattag cagaagcgta
    20821 ttctaaagat gtgctgagcc cacttttagg aaatgaggaa gggaaacaat accgatattg
    20881 gcggatccag tccttgcttt acaatggcgg ctatctctat cataatggag gtaacatgtt
    20941 ttatcctgat attgtcaaaa tgaatgaaaa aataatagag acctatgaaa aagtctttaa
    21001 gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa tgaaagaagg agtgcgcaaa
    21061 tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg gtacacaacc aaagactgga
    21121 tgttacggtt tgtgaaggga atgtttatcg gttcaggatt tatcttgcca ggagtcagcg
    21181 gtggtgcatt agcagctgtc tttggcatgt atgagcggct gatttctttt ttagcccacc
    21241 ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc tgttggtcta ggcggagtga
    21301 caggcatctt tctactttct tttttagtca gttttttatt aggtgcgtat gaaacaacga
    21361 tattatggtt ttttgtcgga tgtatcgctg gaactgttcc tgcgttatgg aaagaagcag
    21421 ggaaaaaagg aagagatact tctgatgtga tcatcatggt tgttactttt gttctggctt
    21481 atttgttttt attatacggc gctcaattat ttgacgggca agtcgatcag aattttggaa
    21541 catggctgat tgccggcgga ttgattggtt tgggtatgat cgtcccagga ttgagccctt
    21601 ctaatttttt ggtgtatatg ggattgtata aagcgatggc tgacggattt aaagaagtac
    21661 gacttgaagt gattgtgccg atcggaattg gtgggattct ttgtgttctt ggattgtcaa
    21721 aaatcatgga ttacattttc tcaaaggcat atagcaagct attccatttc attttaggtg
    21781 tagtttttgc ttcaacgatt atgatcattc ctaccgatta cgcgggattg ggagttgctg
    21841 gggtactagc ttgtcctgtt ctttttatag ccggtacagc actaggttgg tggatgagcc
    21901 gtttggagga acaatataag taaagataga ttgataataa aaacagcaca cgggtgattc
    21961 aaacaaaaag gttggcatct tttttgtaaa atcattcgtg tgtttttcta taaattttat
    22021 aaaaacaagt tgtttttaat cttggaacac gctagactta aacacgtgat tatttttgaa
    22081 agttggcgga aaatggagaa aaagaaaatg aatggactta cacagcaaga ggtagttcag
    22141 aaaatggctt ctggccagca aaatgactac caggaagatg catcaaaaag tacaaaacaa
    22201 atattcagcg ataatatatt gacgttattc aattttctga actttggtat tgggatttgt
    22261 ctgttatttg taggtgcgta ttccaacatg gcgtatttag caattattgt cgtcaatatc
    22321 gttattggaa tttatcaaga aatacatgca agaaatcttg tacggaaatt atcgattgtt
    22381 tcccaatcag aagtactagt gatccgggac ggtaaagaac aatcgatttc tactaaagag
    22441 ctggtattag gagatttggt aaaaatcgga gcaggagaac agataccagc cgatttacag
    22501 gttatatctg gacgggcaga agcaaatgaa gctcttttga caggcgaatc tgatttgatt
    22561 gtcaaagaaa ctggagctga attgctttct ggaagctttt tgacaagcgg ttctgtcatg
    22621 gctgaagtta tccgagtagg tgcagataat tatgctgtcc gcttgactag cgaggcaaag
    22681 actcacaaag ctattcgttc tgaacttgta gattcgattc gcaaagtatc caaattcact
    22741 agtttcgtga ttattccttt ggggatcata ctatttttgg aagcactgtt catccgcgat
    22801 gcttctatca aaatttctgt tattgcatct gcagcagctt tgttaggcat gcttccaaag
    22861 ggtttggtat tgctaataag tattgcatta acgacagggg taaccaaact ggcaaaaaaa
    22921 cgaatccttg ttcaagacat gtattcaatt gaaacgctgg cccatgtcga tacattatgc
    22981 ttggataaaa cagggaccat tactgaaggg aaaatgagta tccagcgggt agaagcacta
    23041 catccgctgt acgagaaagc catcccagaa attatgggga cgtatctggc agaaagcagt
    23101 gataacaaca tcactatgcg ggctatgaga gattattttg agctttctaa tcggtatgat
    23161 gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg gcgcgatgga atttccagaa
    23221 ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg aagaagatcg gcttccggac
    23281 agtctaaaaa cagcccaagc gaatggctat cgcgtactta tgttggggat cgctccaaat
    23341 cagcaattga acgaagaaaa accagcattt gttgagccgg ttgctctgtt tgaaatcgat
    23401 gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc ttcatgagga aggtgtggat
    23461 ctgaaagtga tttctggaga caatcctttt actgtctcca atatcgctag acgtgcagga
    23521 ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta ctgaagaaat ggaagtgcga
    23581 gaagctgctc atcgctatac ggtttttgga cgagtgagtc cgcagcaaaa gaaattgctg
    23641 gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga caggcgacgg tgtgaatgat
    23701 gttttggcgt taagagaagc agattgcagc attgctatgg cagaaggtga tggagcaaca
    23761 cgtcagattg cgaatctggt attgttggac tctgatttta ctacattgcc agaagtcctg
    23821 tttgaaggac gccgagtggt caacaatgtg acgaaagtct caggcatctt ttttatcaaa
    23881 acgatttatt catttatttt atcgattata tgtgccgtta cagcaattgg atttccgttt
    23941 atccctatcc aaatcacgct gattgattta gcgattgaag ggtatccttc cttcttttta
    24001 tcttttgaac aagataaaag aaaaatcact taccgttatt tgcctacagc acttattaat
    24061 gctttaccaa atgccttgct tgtagtagtg aatatcattg cggtgtatct acttggaaaa
    24121 acattcgcat ggggaaatgc tgaaactact actttgatgt attacttgct gattgggatc
    24181 agttgcatgg cagttgtaaa agcttgttat cccttcaacc cattgcgcat cttcctagca
    24241 gtaacgacga tcgtagggat atatgtagct gcaatgctgt tccatggaat tttggaagta
    24301 aacttagggt tgggagagag cgtaggatac ttctttgtat tcatgctcgt aaatattgtt
    24361 cttcgagtat tgtactggaa ggctgatatt caaaatcagt tactgaaaag agtagcaaat
    24421 tgatgcttaa tgagatagaa atgtgttata cttgaaaaag caatgaaata tgatacaatc
    24481 aaacatgtat aattgtgtcc tttaaaatcg cactgatttc agttagtgcg attttttacc
    24541 ggttattctc ctgaaatatc ttctgttaaa cccaaaagat atcaatagag cagggggtga
    24601 aaaaatggct caacggattc ctcaagaggt tatcgaaaca gtacgcagtc aaacgaatat
    24661 cgttgaggtg attggacaat atgtccagtt gaaaaagtct ggtaaaaatt acttggggct
    24721 gtgtcctttt catgaagaac gtacaccgtc tttttctgtt gcagaagaca agcagatttt
    24781 tcattgcttt ggttgtggta aaggcggaaa cgtgtttact tttcttcaag aactagaagg
    24841 tctaagtttt ccagaagcag tggtaaaagt ggcagacttc gaacagatac cgttagaaga
    24901 tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa tctgctacgg ggaaactgat
    24961 tgctgctcat gaaaaagcag cggaaatcta tcatcacatg ctattgcata caaaagctgg
    25021 acagccagct ttagaatatt tgcaaagtcg tgggttgtct ttagaattaa ttgaagaatt
    25081 caacataggt tttgcaccta atgagcgttc ttttcttcag caagtattca agaatgaatc
    25141 aatttcaaat gaattgttag aacgctctgg attgtttgtc acgcatgatg atggccgtat
    25201 gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt aattctcaag gccagacgat
    25261 cggtttttca ggacgctggc ttgcacctga aaaagggcag gaaaaagatc agcccaaata
    25321 cttgaatagt ccagaaacag aactgttcaa taaaagacaa gttttattta atcttgataa
    25381 agcaagaagc gatatccgga aaaacggaga aattttgttg ttcgaagggt ttatggatgt
    25441 gattgctgca tggcaggctg gtatcaaaac tggattagct tctatgggga caagtctaac
    25501 agctcaacag atccaacaaa tggaaaagct gacgaaagaa ttggtctttt gctatgacgg
    25561 agacaacgca ggttttgaag caaccaatcg agggattgaa ttgcttcggc aaaacagtcg
    25621 attagcactt tctgtcgtag tcgttccaga aaagcttgat cctgatgaat atctccgtaa
    25681 gtatggagaa gcatctttcc aagaattagt ggctcatggg cgcgaaacag tctttacttt
    25741 taaatcacgt tatttaaaac aagggaaaaa tttagataat gaaaaagaca agatcgaata
    25801 cctcgaagaa ttactagttg aacttagccg agttgtttca ccaatcgaac aagatatgta
    25861 tctatctcaa ctgtcaacag agtttcagat cggcagagag acgatacaaa aacaactgcg
    25921 cgatcttcga aggacaaatc agcaaaatcg tcaagaacct caggaaagtc aacacactca
    25981 ggttacgaga caagaaactg cacggaaaaa gagacctttg acgcaagtag aaaaggcaga
    26041 gcagattcta ttatatcgtg catttaaaga agtttctgtc cgtaatattt taaaagaacg
    26101 agaatttcag tttattcatg atgcatatgc agaagtttat tttttattcg atgcatatgt
    26161 ctcacaacat tatgaattca accttgcaga gtttttagac tttttgcaag atgaaaattt
    26221 aaggcggttg gtggtggaaa tcgaatattt accggtggca gaagaaagtt cgccacgaga
    26281 aatccaagac ttgctagatg tgatcagcaa atctggctta gctgatgaaa taacattaaa
    26341 aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa cagcttgagc ttgaattgac
    26401 gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc aaatagctac tcaagggggg
    26461 ccttctttta tggcaaatgg aacagataaa aaaacgtatg aagcagcagt agcagctttc
    26521 atcaaagaaa acaaacccaa acgacaagtt gtatatgatg atttatccaa tcagcttgct
    26581 acaccattca ctttgaatgc agatgaaatg gacaaattga tccaaaagat tgaagacgca
    26641 ggtatcagtg tggtggatga aaatggtgat ccatcaattc acagcttgaa gagtgctgag
    26701 aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc caaccggtgt aaaaatcaat
    26761 gacccggttc gtatgtatct gaaagaaatt ggccgtgtct cccttttaac tgcggaagaa
    26821 gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag aagcgaaaca gcgtctggca
    26881 gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt atgtaggacg aggcatgcag
    26941 ttccttgact tgatccaaga aggaaacatg ggactgatga aggcagttga aaaatttgac
    27001 taccgcaaag gatttaagtt ttcaacctat gctacatggt ggattcgtca agccatcact
    27061 cgagcaatcg ctgaccaagc acgtacgatc cgtattccag ttcatatggt tgaaacaatt
    27121 aataaattga tacgtatcca acgtcaattg ctgcaagacc ttggaagaga acctacgcca
    27181 gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag tccgagaaat cttgaaaatc
    27241 gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag aagatgactc tcacttaggt
    27301 gatttcatag aagaccagga agcaacaagt ccagcagaac acgcagctta tgagttacta
    27361 aaagaacaat tggaagatgt actagataca ttgactgatc gggaagagaa tgtccttcgc
    27421 ttacgttttg gtttagatga tggccggacg agaacattgg aagaagtagg gaaagtgttt
    27481 ggtgttactc gggaacgtat tcgtcagatc gaggctaaag cattaagaaa attgcgccat
    27541 ccttctcgtt caaaacaatt aaaagatttc ttagaataag cgtggactat tgaccagaag
    27601 agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa a
//
LOCUS       DASHFF010000031        27497 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.031, whole genome shotgun sequence.
ACCESSION   DASHFF010000031 DASHFF010000000
VERSION     DASHFF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.031"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            129..746
                     /locus_tag="U2I75_001519"
     CDS             129..746
                     /locus_tag="U2I75_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696663.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1766..2935
                     /locus_tag="U2I75_001520"
     CDS             1766..2935
                     /locus_tag="U2I75_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1696664.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3408..3962
                     /locus_tag="U2I75_001521"
     CDS             3408..3962
                     /locus_tag="U2I75_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1696665.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4135..4608)
                     /locus_tag="U2I75_001522"
     CDS             complement(4135..4608)
                     /locus_tag="U2I75_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1696666.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4620..5156
                     /locus_tag="U2I75_001523"
     CDS             4620..5156
                     /locus_tag="U2I75_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1696667.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5196..5567
                     /locus_tag="U2I75_001524"
     CDS             5196..5567
                     /locus_tag="U2I75_001524"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1696668.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5851..7122
                     /locus_tag="U2I75_001525"
     CDS             5851..7122
                     /locus_tag="U2I75_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1696669.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7367..8392
                     /locus_tag="U2I75_001526"
     CDS             7367..8392
                     /locus_tag="U2I75_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1696670.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8686..9795
                     /locus_tag="U2I75_001527"
     CDS             8686..9795
                     /locus_tag="U2I75_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1696671.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9878..10774
                     /locus_tag="U2I75_001528"
                     /pseudo
     CDS             9878..10774
                     /locus_tag="U2I75_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10790..11614
                     /locus_tag="U2I75_001529"
     CDS             10790..11614
                     /locus_tag="U2I75_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1696672.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11646..12956
                     /locus_tag="U2I75_001530"
     CDS             11646..12956
                     /locus_tag="U2I75_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEN1696673.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            13104..14363
                     /locus_tag="U2I75_001531"
     CDS             13104..14363
                     /locus_tag="U2I75_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1696674.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14373..15245
                     /locus_tag="U2I75_001532"
     CDS             14373..15245
                     /locus_tag="U2I75_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1696675.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15257..16087
                     /locus_tag="U2I75_001533"
     CDS             15257..16087
                     /locus_tag="U2I75_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1696676.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16127..18310
                     /locus_tag="U2I75_001534"
     CDS             16127..18310
                     /locus_tag="U2I75_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1696677.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18371..19591
                     /locus_tag="U2I75_001535"
     CDS             18371..19591
                     /locus_tag="U2I75_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEN1696678.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19588..21048
                     /gene="gtfA"
                     /locus_tag="U2I75_001536"
     CDS             19588..21048
                     /gene="gtfA"
                     /locus_tag="U2I75_001536"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEN1696679.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21125..22222
                     /locus_tag="U2I75_001537"
                     /pseudo
     CDS             21125..22222
                     /locus_tag="U2I75_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22544..22753
                     /locus_tag="U2I75_001538"
     CDS             22544..22753
                     /locus_tag="U2I75_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1696680.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22746..22895
                     /locus_tag="U2I75_001539"
     CDS             22746..22895
                     /locus_tag="U2I75_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEN1696681.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            23049..23714
                     /locus_tag="U2I75_001540"
     CDS             23049..23714
                     /locus_tag="U2I75_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1696682.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23998..25083)
                     /gene="xerS"
                     /locus_tag="U2I75_001541"
     CDS             complement(23998..25083)
                     /gene="xerS"
                     /locus_tag="U2I75_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN1696683.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25417..25650
                     /locus_tag="U2I75_001542"
     CDS             25417..25650
                     /locus_tag="U2I75_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN1696684.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25647..26054
                     /locus_tag="U2I75_001543"
     CDS             25647..26054
                     /locus_tag="U2I75_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN1696685.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(26505..27014)
                     /locus_tag="U2I75_001544"
     CDS             complement(26505..27014)
                     /locus_tag="U2I75_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696686.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
     gene            27130..27497
                     /locus_tag="U2I75_001545"
                     /pseudo
     CDS             27130..27497
                     /locus_tag="U2I75_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         9469 a         4093 c         5125 g         8810 t
ORIGIN      
        1 aaatggaacg ctcttcggct tttcggtgtc tactgaaatc aaggtattaa tgggaataac
       61 agcctaagat atttggagtt cagagagggc gcgtttgatt ttcaaacttc gcaacagaac
      121 cgagaacctt gggttttcaa gtgctttttg gttcattaat cttaaccata gtgtttttaa
      181 agtggaaaac aagcaacaaa aaaagtatgt ggagccttgt aggaatagtt cttattattt
      241 ttactgtgac tgcagcaaca gaagcaaatt attcgcataa aattcagtcg tataaaggtc
      301 gtttagttat gaccaaaaaa gatgttacac gttatacatc ctcaagaact ggaggcctat
      361 atgactatgc accattagat gcattgaaat ataaagatca tttaaagaaa catgaagtaa
      421 aagttggtga tcggtgggag aatgcatctc ataaagcata taattccaaa atagtctata
      481 cagtagaaga tgccgatgag caaaatatca cattacctgt attcaattat tggggaacag
      541 ttgtgaaagt aaatgggaaa aagagcacta tagaaaatga caatggttta gtgtctatta
      601 aaggtacggg gaaaagaact atagttgagg tttcatctac gtatccaact acgatgattc
      661 tatctttatt aattagttca ataactttta tattcttatt tacacgttat ttaataagat
      721 gctgcggtga aatgaaaata gattagatag atattataaa tatgttgatt ctattttcat
      781 agcaatggag aaaatacatt agcaatatat aaaagaaggt atcaagagtc acagtgaatt
      841 tattccgaaa agtgaaaaga gcaaatataa aatttaatga aaatatagaa ctaaatagga
      901 aaacgtagac gtaattttct acgttttcct tttcatatat attaaatatt acggaaaatt
      961 atataaacta atttagtgtt ttgtttgtaa ttcctaattt tgctccactt ttagataatt
     1021 tttaggtcta tttaactcat cagacattct atgctcattt ttctccaaat ttgtttcaac
     1081 tctttcgtac ttaattttgc gtatttgaaa attcttcttt tagagtactt ttattacgat
     1141 actagtatga tatatatcta tagcaaacag ttaaaatctt cctaaaataa gcgaatagca
     1201 ttcctaaaac ctataaaatt tttagatgaa gcgacaagtg gacaattaat aaactacttg
     1261 aacatgaaat tatggatcac ctttcaaatt tagaaaatgg aattcttata cttattgcgc
     1321 aacatttatt gattgcaaaa ttatgtgaca aaaaaaacta gtagaagaaa gaataatggc
     1381 ttgataataa agtactacct attaaaggta gtactttatt taatttagta taaattaaca
     1441 tactactata ttttaggttt catattatat aaattaataa tatactacca tttgttggta
     1501 gtataattta aaggtaataa gttattcttt tattactatg tttgggtttt atagcaaaga
     1561 aagtttaata agagtttgga cctagcaaac aatattaatg aggtcattaa atcttattat
     1621 ctttagtgat ataacgaaat atctacacag acaaaatgat agcgtaaaat attttgtgta
     1681 aatagatatt tagggtagaa aaaaagaaag tccattctgt aaaattaaag ttaccacaac
     1741 cgaaatttta taggaggact ttctcatgcc caattttact acagaaatta tggaaacact
     1801 aatcaataaa ggtgatttag atgaattatt tcgatgtcat ttagaattag ctgttaattc
     1861 attattgcag gctgaattaa cagcttttct tgattatgaa aaatatgatc gtgctgggtt
     1921 caattcaggt aattcccgta atgggaatta ttcacgttca ttcagaacag agtatggaga
     1981 attaaatttg gtaattccta gagatagaaa tggtaagttt tctcaacaaa ctttacctgc
     2041 ttataaaaga accaatgact ctttagaaac cactattatt cagctatttc aaaaaggaat
     2101 tactatgtct gaaatctctg aattgattga aaaaatgtac gggcatcact atacgccaca
     2161 aaccatttca aatatgacta aaatagtttc tgaagatatt attgccttta aagaaaggtc
     2221 cttagaatcc agatactctg tcatttttat ggatgctact cacattcctt taaaaagaca
     2281 aactgtttca aaagaagcag tttatattgt aattggcatt cgattagatg gaaccaaaga
     2341 ggttcttggg tttagtcttg ctccaactga atctgcttat gtttggaaag agattcttca
     2401 agacctaaaa gaccgtggtt taaaagaggt tttattagtc gtaactgatg gtttaagtgg
     2461 cattaacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca
     2521 tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga
     2581 tttcaaatta gtttatcaag caagttcaaa agaagaagca aataatcaaa taaggtttat
     2641 gatagataaa tggaaaaagc agtatccacg agtggttaaa ttgctcatga atcctgctat
     2701 attaaccttt tataatttcc ctccgtcaat cagaagaact atttattcaa ctaatttgat
     2761 tgaaggtttt aacaaacaat taaagaaata taccaaaaga aaagagcaat ttcctaatga
     2821 agaatctctg gagagatttc tagtatctca attcaacaac tataatcaaa agtttctatg
     2881 ccgagttcat aaaggtttta aagaaataca tgatacatta gaatcaatgt tttaacttga
     2941 tcaacagaat gggcttttcc atttacacaa aattattgac gctcccgaca aaattatgat
     3001 cttgcgtaaa gttataaaat attaataaaa atattttatt aatgaaaagc ttggtagcac
     3061 ggttattcat atgctaattt atttcacttt atagtaaggg gagttcttag tatattgttc
     3121 aaacttaaat ttcatataat ttaattatgt aaattagcta ttctaagaac ttctacggat
     3181 aactgcccgt ctaaaattta aataagagct tagaatcttc atataagtaa ggttaatttt
     3241 tttgtacaaa aaagtcggaa tattctgagt tactgaaatg atataaaatc ataaactaaa
     3301 tcccttgatt ttattgactt tcaaaagttt atatatataa gttaatgatt atggtaaaat
     3361 atatacataa tcattaactc agaatatttt taagaaggga aacaatagtg agctataatg
     3421 tacaaccgtt aaggacaaat gaagagatac aagattttca gttttggcta agaagaacaa
     3481 gtaatccgga aagagatagt tttttatttt tgtttggaat caataatggg ttacgtatgt
     3541 cagatattat aggattaaaa gttggagata tacgaggcaa aagtaagcct attattgttg
     3601 aacataaaac aggtaagcga aaaccgattt ttatagataa tttgagagaa gaaattttat
     3661 tatatactga aggtaaagaa gaaaatgatt ggttatttcc gagtcggcaa caaggtcgac
     3721 atataacaag ggatcgtgtc tatcagatat atgcagatat agcagaaaaa ttaggacgag
     3781 atgatatagg aacacataca ttaagaaaaa cttttgggta tcactactac aagaaaacta
     3841 gagacattgc taccttaatg tttattttta atcatagctc gcaagcaatt acaaaaaggt
     3901 acattggtat aactgaagat gaaataggag catcacttcg tggatttaaa ctaggtgtat
     3961 aatttaaaaa tttttataaa cagtaaagac aaagattaaa agctattttt atctagaacg
     4021 attttatttc ataaagttcg cggaataaaa gctggggtta ctggtacttg gtataggttc
     4081 gtgttatata aactaagtaa ttaaataaca tttttaggtt ggaaaaggat gaaattaaaa
     4141 aaagagaatc aatacggtct caaaaacgca aacgctgcgg aataagaaaa atactgataa
     4201 ctcaacgttt agcagactat ctaataggga tagtctgctt ttttcgtttt tgaccatact
     4261 tttgaccttc tgcaaacttc cttgcttttc ctctgtagaa ctcatgaaat ctgcaaaacg
     4321 aattgccgtt tcctctgtct gctttttcgc gatatgggtg tatatatcat ccatagtggt
     4381 tttaatatct gtatgtccaa gtcgaacata cacttctttg acagacgcac cagattcaaa
     4441 gagcagacta caatgggtat gacgaaaacc atgaagtgtg atatgtttta agttattctc
     4501 tttaatgatg attttcaaat tgtgatttac atagtctaaa taaaggggct tattttcttc
     4561 tgatgaaaac aaaagttgtt caccatcttt tataggtttg attctcattt ttagatatta
     4621 tgcccgcacg tattcatcac atttctattg tgaatcgctt tattcgaccg acatttgact
     4681 tttatcataa tatactcggt ttaaaattac taatgaaaac aattaatcaa gacgatcata
     4741 ccatgtatca cctgtttttt tcagataatc accaccgtgt gggaactgaa ctgacttttt
     4801 ttgaagtaca agaagggaac aaccagtttt ttggaaccaa tacgattgaa cgaacgatac
     4861 taaaagttcc ttccgaagca tcattgcatt tttgggaaat atattttgaa acacaaggcg
     4921 tttgccatta tggaatcgaa tcattttctg gacgacctat cttacgtttt gaagggccag
     4981 atgcaacacg attagcttta gttcccttac gcgaatttga agatattgat gattatttcc
     5041 cgtatgtcac agaccagatc cctacagagc atgccatttt aggaattgat gccattcaac
     5101 tacgcgtaca atattccgat gcgactgaac gagagttatt gtcggtaggc tggtagcaca
     5161 aagaatacgt cagtttcttt tcgggtcatc aacgagtgac aatcttagaa aatcaagaca
     5221 ctcagttcta tcaagaagtt cagattattc aagatcgtga gaatccagtt gccattgaag
     5281 ggattggtag cgtccatcat gttgcgtttg gcgtagaaaa taaatctgat ttacaacgca
     5341 tcgacaaaca attacaagag cgcaatttta taaattcagg aataaaagat cgtgaatttt
     5401 ttgtatcact ttattatcgt gacccaaatc aattattgat tgaaattgct acaggggaag
     5461 gaaacctaga tgcaaaagcg tatgaaaatc aaagtccacg ctttgaagaa attcctttat
     5521 ttttaccgca gtacttaaat ttcattcgtg aacaagtgga gcaataatta gctaatcaaa
     5581 tccatgacga agagtaagag gttgactcat aagtcaaaaa ataaagaaaa acgcatcaaa
     5641 tccattctaa agaatgttga tttgatgcgg ttcctatttt tataaactat cattttcgac
     5701 taataaaact acttatgagt cagcctctct ttttttcttg atttttatac ctctttctag
     5761 gtaaactaaa catctgccaa aaataccgtg tctcattgaa acattctcaa ctatttcaaa
     5821 ataaagggga gtaccaaatg aacgagtata ttgaaattat ccacgcaaga gaaacaactt
     5881 aaaaatgtca gtctcaaaat tcccaaaaat aaaattagta tttttaccag cgtatctggt
     5941 tcaggaaaat catcgattgt ttttgatact attacccaag aagctggtcg tcaactgaat
     6001 gacacctatg atagttttac ccgtttattt ttacctaaat acagtcgtcc agaggttgat
     6061 gagattgata atctttctac ggcgattgtg gtcaatcaaa agaagattgg tggcagtgcg
     6121 cgctcaacgt tagggacgat tactaacatc aatgcgttat ttcgtgtgtt gttttcatgt
     6181 tttgcaacac ctagtttagg atatgcaaat gcttattcat ttaatgatcc tagtggaatg
     6241 tgttctgtct gtcagggaat cggtaagacg attgcatttt taataggatt gctttatttt
     6301 tcggctattg ataatgctga ttttgtattt tctgatccat ttaccgcaga agttttgtct
     6361 ggaattgagg aacaggcaaa gaatcatggt tattttgtat tggtgcataa tgttaattca
     6421 gcagaagatg ttaaaactat tcagagaaat tggcgttttg aggggttcat tgttgttggt
     6481 gtacgtgtaa aagaattttt ggaattaaat aacgtttttg atggtcctgt tgtttatatt
     6541 gatacgcatc tttcaaaaga gacattaaga gaacttgaac aacaaatgaa tcgtttattt
     6601 gtaaatacaa atgattacca agctagtaca ttggcagtgg aacatttgat tcattatggt
     6661 catgagaaaa tagcgtttct ttcttttgac tacattgtag gtgaaccaag tgttattcaa
     6721 gaacgacatc aagcttatat cgatacatta aagaaatatc atttaacctt taaatcgcat
     6781 tggatatata acaactctga atttgagcga atttatcaag aattagctga atttactgca
     6841 attgttgtta ctggagatta tttagcggct aaattagtaa aatttttaaa aaataagaat
     6901 aaacgagtga tggaacaatt atcgattatt ggatttgatg atattacttt tgcagaatta
     6961 atggatcctg cgttgacaac aatacgacta aatccaggta acaagggggc gattgctttt
     7021 caccaactga ttggactggc caaacaagaa gaaataaaaa atcaagtact actattagag
     7081 ggagaattga tttctcgaga ctctgttcac aaaatcaaat aaaaaagctc tgtgaaataa
     7141 gtatagttaa caataaaata tccgaacgat agagagaatg aactgtgatc tagcagttcc
     7201 tcgtcgcaaa ttcactagaa ttagcattat ttactctaaa ttgttcggat attttttctt
     7261 gtttttaatt atgattacag tgcctatttt taacgaaatt tgcttatttt caaaatgtat
     7321 gattatatgt tagtaaacac tttcaaatat gaaaagagga gctattatgg taggaattag
     7381 ggatgttgca aaacgggcgg gtgtgtccat tgcaacagta tcgcgcgtgc taaatgagga
     7441 tccgacactt tcagtaaccg atgaaaccaa acgaaagatt gcagaatcgg ttaattttta
     7501 tcattatgtc aaaaaaagtc ccgttgcaaa aaatttaaca aatattgcat taataacgac
     7561 agtttctgaa gtagatgagt tggaagatcc ctattttcga acgattcgca gagggattca
     7621 agtcgaagca gaacaaagca aagtcaattt gaagaaaatt attcgtttgt cagaaacacc
     7681 gttggagcaa gacgatttgc taaaatgtca aggtgtgttg attattgggc aggtgctatc
     7741 ttctgtgatt gaagaagtat cagcaattaa ttcgaatgtc gtagtgattg atgaccctag
     7801 tattgaagct gaagtagatg cagtttatac tgatttaaca aaagcaacga atcagcattt
     7861 agatcgattg tataaaaaag gacaccgaaa cattgcgttt atcggaggag gacgtgttcg
     7921 tttagatagc caaggaaata aacacgtgtc agatgatgat tatcgaaaaa tagcctatga
     7981 gacgtggatg aaacaaaaag gattagaaaa atatagtcaa atttatttag aaggctggac
     8041 cacacttgac ggaatgcagg cagcagattt acttttgaaa aaacatgcta aagataagtt
     8101 gccgactgct atcatggtag gaaatgatcc tattgctgtg ggggtttatc gttcacttca
     8161 aaaaaatggt ctacatattc cagaagatat ttcgattgtg agttttgata atattgaagt
     8221 tgctgaattt ttaacacctg cattaaatac ggtgaatgtg aatacagaag aaattggaaa
     8281 actagcagtt cgcatggtta atgaacgaat aaaagaagtt agaaatgttt cgatcaaagt
     8341 gattgtttct aataatatta ttgttagaga aagtgaaaaa aatatcgatt aaaaaagtaa
     8401 acatccaaaa aagggtgttt acttttttgt ttactaatat ttttagtaaa aaatctcgac
     8461 aaaacattaa gtaaacagtt atggtgttgt taacaaaaag aaaacgcatt caaaacagga
     8521 ggaattaaga tgaaacattt caaaaaaata gctttaacag ggatagctgt tttaactggg
     8581 acattactcg cagcatgtgg tgggggagat agtaattcta aagacgggca agtaacttta
     8641 agctatgcga gttgagatac tgaccaagct ccaggactaa gaaaaatgtt agatgaattt
     8701 gaaaaacaaa atcctgatat caaagtagaa atggagacaa cgccttggga tcaatattgg
     8761 gttaaattag aagctgcaac aacgggaggt aatatgccag atgtagtaac catgcactct
     8821 agcgaatcct ataaatatat gtcacagaat gctttgatgg gacttgatga tgttatcaaa
     8881 gaaaatgata ttgacatggg aaactttact gaaggaattg ctgatttcta tacgcttgaa
     8941 gacgaactat acgcaatccc taaagatgct tctgtagtgg ggctatggta caacaaagaa
     9001 ttatttgata atgcaggtgt cgattatcca gatgaaacat ggacatggga aacactcaga
     9061 caagctgcaa ttgatttaac agatgaatca aaaggcatct atggttttgc atcacatatt
     9121 ggaacagaga ctggctactg gccatttatt tatcaaaatg ggggagaagt atttgccgat
     9181 gacaatacaa aatcaggttt gaatacccca gaagcacaag aagctttagc tttttatcga
     9241 gatattgcac ttgttgatca tgcttcacca acaattacag aattacaaga tcctgataag
     9301 ttaagtcgtt ttcaatcagg tagattagca atgatgattg aaggaaactg gtttgcaccg
     9361 ggatttttga aaaatgacta tctagttgaa aatgttgctt ctgccgtgtt acctaaggga
     9421 aaagttaatg caacaattac aaatggactt ggttgggctg ccagtgcgag tacaaaatac
     9481 ccagaagaag tgaaaaaact aatggcattt ttagctagtg aggacgccaa taaaatccaa
     9541 gcggaaacgg gggcatcaat cccagcacta aaaggctttg aagacgagtg ggctgcttct
     9601 tcagatacaa ttgatttgag tgcttttgga aaaatgttgg agtatggagt ccctcgtcca
     9661 tacaataaag atggattacg agctgaacaa gcagaagttc aaattattaa ccgtattctg
     9721 tctggtgaag taactgttga agatggaacc gctcaaattg aaaaagaagt caatgcgata
     9781 ttgagcggaa attaaattag ttaattatac aatatacagg cgaaaggatt ctatttcgtc
     9841 tgtatattaa tggatgaata ccaaaaaggg ggatattatg aaaaatcgaa tttttggaaa
     9901 atatcgtaaa gatcaagtga tatggggcta tatttttatt tttccaacgg cattaggatt
     9961 aattttatta aatatatggc cagcaattca aacagcaatt ttaagttttc aaaaaacggt
    10021 agggttcggt gatactgaat gggtcggatt aagaaattac caaaaaatgt ttgcagacgg
    10081 tgaagttttt cagtctttat taaacacatt aatttatgca gtggtttcga tacctagtat
    10141 tgttgtatta tccttgttgg tggcagtttt aatgaataga aaagttaaag gattgtcgat
    10201 ttatcggaca atttattttc taccagtcgt tgctgcacca tccgcagtcg caatgatttg
    10261 gcgctggatg ttcaataatg attatggact gattaataac atgttggcta aaataggtct
    10321 cgatggacca gcatggttaa cggatccatc gattgcgatt tattctatta tcattgtagg
    10381 agtttggagt gctattggtt ataacatggt gttattacta gccggcttat aagaaattcc
    10441 taaaaattat tatgaagcag cggaaattga tggtgctagt ccaatccagc agtttttcaa
    10501 cattacgtta ccattagtta caccaacatt attttttgta gttgtaacaa cagttatcaa
    10561 tgctttccaa gtatttgatg ttatttttat gatgattact cctaatagta ccgccatgtt
    10621 taaatcacaa tcattagcct atttattcta taaacattca tttattttaa atgacaaggg
    10681 atatggttcg gcaatcgtta tgttcctttt agtgattata ttaattatta ctgcaattca
    10741 aatgaagttg cagaaaaagt gggtaaacta ttaaggaggc tatcaaatta tgaaaaataa
    10801 atttttttca aatgcgccaa tccatatcat gcttatttta ggtggtattg caatggcatt
    10861 accttttatt tggatgattt taacttcagg gaaaacgttg gttgaatcta cccaaattcc
    10921 acctacaatt ttccctgaga aatttcagct aagtaactat caacaagtac ttgatttatt
    10981 accattcatg gctttttata tcaatacatt tttaatggtt gttggacgag tgattggctc
    11041 ggttttattt tcagcaatgg ctgcatatgc ttgtgcgcgt ctaagttttc cagggaaaaa
    11101 tatccttttc agcttagtac ttattcaaat gatgattccc ggttccattt ttattactcc
    11161 acagtattta ttagcacaaa aattgggtat tttaaataca attccagcat taatattacc
    11221 aggggtagtt agtgcgtttg gtacgttttt attaagacaa tttttcttag gtttacctga
    11281 tgaattggag gaagcggcct atttagaagg tgccaatatt tggacgatct ttttcaaaat
    11341 tatgttgccg cttgctaaat caggattggt tgcacttgga atattcacag cgttgtttgc
    11401 atttaaagat ttaatgtggc cattgattgt caatacttca attgataaga tgcctttagc
    11461 tccgggatta gcaaacttac aaggacaata tgccacgaac ttcccacaat taatggcagg
    11521 ttcgttatta gcaatttggc caatagttgt gatattcatc gttttccaaa gaaaattcat
    11581 tgaaggaatt gctacaacag gtagtaaatt ataaaactat taattagtga ttggagagta
    11641 agtacatgac aaaaattaca tttattggtg caggaagtac tatttttgca aaaaacgtat
    11701 taggcgatgc gatgttaact ccaagtctac aagatgcaca tattgcattg tacgatattg
    11761 atgaaaaacg actaaaagaa tcaaatttaa tgcttaaaaa tattaataaa aattcgaatg
    11821 aaaatcgtgc tgatattgtt tcttattatg atcgcaaaga agcgttaaga gaggctgatt
    11881 ttgttatcaa tgctattcaa gtgggtggtt ataaaccttc aacagtgatt gattttgata
    11941 ttcctaaaaa atatgggttg cgacaaacta ttggtgacac catggggatt ggtgggattt
    12001 ttagaaccct acggacacta ccagtaatgt tagactttgc taaagaaatg gaagaagttt
    12061 gtccaaatgc ttggttattg aactatacaa atccgatggc gatgttaacc atgggtgttt
    12121 taaaagcgac gagtattaag actgttggtt tatgtcatag cgttcaagta tgcgtaccag
    12181 aattgtttga gcatttagga atagccgatc aatatgactt aaatgacttt caatggaaaa
    12241 ttgcaggcat taatcatatg gcattcttgt tggaaattac aaaaaatggg gaagatttct
    12301 atccaacaat tcgtcagtta gctgcagaaa aaccaaatcc acatccagat tctgtacgtt
    12361 ttgaattgtt gaaacgtttt ggttactata ttacagaatc aagtgaacac aatgcagaat
    12421 accatccata ctttattaaa tcaaaatacc cagaattgat tgacgaatta gggattccgt
    12481 tggatgaata cttacgtcgt tgtgaacaac aaattaatga ttgggaaagc atgcgagagg
    12541 acattgtaaa taatggggag ttaacacata cgagaagccg tgaatatgct tcatatatca
    12601 tggattctat tacgacagga actccgtcag tgattgcagg aaatgtctta aataaaggct
    12661 taatcacaaa cttaccgaca gatgtttgtg tggaagtacc ttgtttagtc gataaaaatg
    12721 gcgtgcaacc aacctatgtc ggtgaattgc caacacaatt agctgcttta aatcggacga
    12781 atatcaatgt tcaagaactg acagtagaag ccgctttaac actagataaa tcaaaaattt
    12841 atcaagcagc gtatttagat ccacatctat catctgaatt gtctttaaca gatatcaaaa
    12901 atttagtgga tgatttaatc gaagcacatg gggactattt acctgaatat aattaaaaag
    12961 aagctgaaag agtaaggaat tttcaattcc ttactctttt cttagttaaa tttagtaaaa
    13021 atattgacaa aacacttagt aaacagttac attagtgtta acaaaaagaa aacgcattca
    13081 aattgtataa ggaggagcat aaaatgaagt ggtctaaaaa aattatttta gcttgtatag
    13141 cactaggagc aacgatctct ttgactgctt gtggaaaaaa tcaggaagat gcaagcggta
    13201 aggtagaaat agagtttttt aatcaaaaaa aagaaatgac tcaaacgatt caggagattg
    13261 ctaaagattt tgaagcaaaa aaccctgata ttcatgtaaa agttgtggat gtaccgaatg
    13321 ctggtgaagt aattaaaaca cgtatgcttg caggcgatgt accagatgtc attaatcttt
    13381 atcctcaaag tatagaattg aaagaatggg cgaaagctgg ttatttagag gacttaaccg
    13441 aagaaccgta tttagagaat atcaaaaatg gatatgcaca acgttttgct attgaggatc
    13501 gtgtatatag cataccatta accgctaatg tctatggttt ttactataac aaaactgctt
    13561 ttgaggaaat ggggattcaa gcaccagaaa cttgggcaga atttgaaaaa atagtagctg
    13621 agattaaaga tcaaaacaaa gtaccgtttg cgattgcagg agcagaaggc tggactttaa
    13681 atggttatca ccaactagct atagcgactg ctgctggtgg tgaagaggaa gcgaacaatg
    13741 tttggcgttt ctcagaagtt aatggaatta atgctgaaag caaagaaatg caaaaagatt
    13801 ttaatcgatt agatttgtta agagaaccaa aagctttgca aaacaactgg caaggtgccg
    13861 gttataacga tacagtagtt acttttacaa aaggtgatgc gttgattatg ccgaatggtt
    13921 catgggcaat gccgatgatt cattcacaaa atccagattt tgaagtagga acttttcctt
    13981 ttccagcaga tgaagcaggt caaagtctaa cgattggcgc aggagaccta gccttatcag
    14041 tttctgcaac ttctcaacat aaagaagaag ccaaacgttt tgtagaatat atgacaacac
    14101 ctgaagcgat gcagaaatat tatgatgtag atggtgcacc gtgtgctgtt gagggcgtta
    14161 tcgagaatac tcaggattca ccattaagtg gtttaactga acttgctttt actgatcgtc
    14221 atttagtttg gttagccaaa gattggaata gtgaaaatga tttttatacc ttaacaacaa
    14281 attatttaca caatggcaat caaaaagcca tgattaacgc attaaatgct ttctttaatc
    14341 caatgaaagc tgacggaagg taggaaaata aaatgaaaat aaaagaaatt ttaaatcgtt
    14401 actgggcaat catttttgta gcaattccaa ttattttgca gatgattttt ttctactttc
    14461 caatggttca aggtgctttt tacagtttaa ctaattggac tggtctgact tataattttg
    14521 attttgtggg tttgaacaat tataagattc ttttttctga tccgaagttt atcaaatcga
    14581 ttggcttcac tttaattgtg acgctttgca tgattgctgg acaaattggt tttggtattt
    14641 tgattgcgcg ggcgttgaac gcaaatatta aagggaaaac attctttaga gcatggttct
    14701 tttttccagc ggtcttatcg ggcgtggttg tttctttagt atttaaacaa atttttaact
    14761 atggtttacc aaaaattggt agtatgttag gcattgaatt tttacaaacc agtcttttag
    14821 gaacaacatg gggagcgatt tttgcagtaa tttttgtttt actttggcaa ggaattgcca
    14881 tgccagtcat cattttctta gcgggttttc aaagtattcc acaagaaatt attgaagctg
    14941 ctagaatgga tggggcgaat gataagcaaa ttttttggaa tattgaaatc acctacttat
    15001 taccaagtat ttcaatggta tttattatgg cattaaaagg tggcttaaca gcatttgatc
    15061 aaatttttgc tttaactggt ggtggaccaa gcgatgccac aacatctttg gggttacttg
    15121 tttataatta tgcttttaaa aacaatcagt ttggttatgc gaatgctatt gctttagtct
    15181 tatttatctt aattgggatt gtttcactgg cacaattaaa attatcgaaa aaatttgaaa
    15241 tctaggaggt ttcccaatga aaacatcaaa agaaaaaaat atttctaaat acttgctttt
    15301 attattaggg ggcttcatga ttttagttcc cttagtttca acgctattcg gttcatttaa
    15361 aacaacaccc gatattatga ataatttctt tcgttttcct aatccagcga ctttggaaaa
    15421 tttccgaaga ttgttagcgg atggaattgg tcattacttt tggaattcgg caatcatcac
    15481 aatcattgca gtagttgctg tgacagcaat tattcctatg gctgcttatt cactagcaag
    15541 aaaaatgact cgtgggaaag catacggatt gatgtattta tttttaattt taggaatttt
    15601 tgttcctttc caagtaatta tgattccgat taccgttatg atgagtaaaa ttggtttagc
    15661 aaatatgtgg ggattaatct tactctatct cacttatgcc gtaccacaga cactattttt
    15721 atatgttggt tttattaaac taaatgtgcc tatcagtctg gatgaggctg ctgaaattga
    15781 tggggctagt cactttacaa cttatttcaa aattgttttc ccaatgttaa aaccaatgca
    15841 tgcgacgact attattctta atgctctatg gttttggaat gatttcatgc taccattatt
    15901 aattttaaat cgtgattcta atatgtggac actaccattg tttcaatata attatacggg
    15961 acaatatttt aacgactatg gtccaagttt tgcttcctat acagtaggaa ttgtcacgat
    16021 tacactagtt tatcttgctt tccaaagaag tattatagct ggaatgagca atggagcagt
    16081 gaaataacca ataaattatt ctcattataa aggagttcat aaacttatga ttatatttga
    16141 caatcacaca caaacatttc atttacaaaa taaaaaaatt agctatctat taggaatcga
    16201 agaaaataat tatttaacgc atctttattt tggaaaaaag attacgcact attctggtgg
    16261 gtatcgctac ccccgaacag atcgttcctt ctcacccaac aatgcaaatg cgcaagatcg
    16321 tttattatct ttagacacat taccattaga atttccagga tatggacatg gtgattttag
    16381 agaaccggcg cataatatta aattaggaaa cggtagtcgt gtaaatgatt ttcgttatga
    16441 tagctacgaa attcaaaaag gaaagcccag cttagcggga ttgccagcct tatatacgga
    16501 agatgatgat gaagctgaaa cgctgattat tcaattgaaa gacgcgatta gtggattgac
    16561 tttacagttg ttctacacaa tttatcaaaa tcacagtgta attattcgtt ctacgcaatt
    16621 agtaaataat agtgatgaaa cagtggagat taaccgctta gctagtcaat caattgattt
    16681 tccaaatcgt ccactcgagt taattcattt aaatggtacc tgggcaagag aacgtcaaat
    16741 gactagagaa aaaattcata cagggataaa agtattagat agtaaacgcg gatcaagcag
    16801 ccatcaacaa aatccgtttg ttgctttagt agatgccaaa acagatgaat ttcaaggaga
    16861 ggcatatgga tttaatttag tttatagcgg taatcacgaa acggtgattc aaaaagatcc
    16921 atttgaccaa acacgggtca ttatgggaat taattcattt aattttggtt ggcaactaaa
    16981 aactggcgac tcatttcaaa cgccagaagt cgtgatggtt tatagtaatg aaggtctgaa
    17041 tacgttatca cagacgtatc atgaattgta taatcaccgt ttaattcgtg gaaactatcg
    17101 tttaaaagaa cgtcccacgt taatcaacaa ctgggaagca acgtattttg attttaacga
    17161 agaaaaaatc atgaatattg tcgatgatgc gaagaacttg ggcattgaaa tgtttgtttt
    17221 agatgatggt tggtttggcg gtcgtgaaaa tgacttcact tcattaggtg attgggttga
    17281 aacagaaggc aaacttgctt gtggtttgga aaatttagcc aagcaagtgc atgataacgg
    17341 gatgaaattt gggatttggt ttgaaccgga aatgatttct gaagatagtg aactatttcg
    17401 agcacatcca gattgggcat tgggtattcc aggtcgtgac cgctcaatta gtcgaaatca
    17461 atatgttctt gatttttcaa gacaagatgt tcgtgataat atctatcaac aaatgacggc
    17521 gatttttgat cgtgtaccga ttgattatgt gaagtgggat atgaatcgaa atatgacaga
    17581 agtctattct gtcttgttgg actcaacgat gcaaggtgaa gtggctcatc gttatatgtt
    17641 agggttatat gaatttttag aaaagttaac aacgaattat ccaactattt tatttgaaag
    17701 ttgttcgggt ggaggcggtc gttttgatgc aggtttcttg tattacatgc cacaaacttg
    17761 gacgagcgac aataccgatg ccgtggctcg tctgaaaatt caatatggaa ccagcttagt
    17821 gtatccaatt tcaagtatgg gtgcgcatgt ttcagcagtc ccaaatcatc aaacaggaag
    17881 agaaacgagt ttaactattc gtggcaatgc tgcgatggct ggtgtttttg gttatgagct
    17941 agatctaacg caattaactt ctgaagaaaa agcagaaatt aaagaacaag ttgcctttta
    18001 taaaaagcat cgccaacttt tgcaatatgg aactttttat cgcttggaaa gtccgtttga
    18061 aggcaatgat actgcatgga tgtttgtctc aaaagaccaa acagaagcca tggtctttta
    18121 tttccgagta ttagcagaag ctgcgcatcc attagtcaca ttgaaattag caggattagc
    18181 agaagaagca acttatcaaa tggaagatgt aacatttgct ggcgatgaat tgatgaatct
    18241 cggtttttat attgactcag aactacatgg tgattatgcg acacaacgct tttatttgaa
    18301 acagatatag tagcttgaat ttacaatata agtgcaacac caaaagagac tcattccaaa
    18361 cgaagaaaat atgttttatt tacacgcatt tactaaaaag cagcctgatt tgaattggga
    18421 aaatgaagaa gtacgtgaag aaatctatgc aatgattaac tattggttag ataaaggact
    18481 aggcggattt agaatcgatg cgatattaaa catcaaaaaa aaactagagt atggcatctt
    18541 tgaatctgat ggagaagatg ggttagcctt cattgggccc tggattctta atcagtcggg
    18601 cattgaagtg tggttaaaag aaatgagaga gcggacattt aaaccacata atagtatgac
    18661 ggtagcagaa gccgatgttc ctaatgaacg tttagatgaa tatattggtg aagatggcta
    18721 ttattcgatg gtttttgatt ttagttatac ggatattgat gtacctgaaa ctggtgaatg
    18781 gtttaaagat agccaatgga catggacgga tatgcgtacc aatattttta cgaatcaatt
    18841 agttacgcaa gataaaggtt ggggcgcctt atatttagaa aaccacgatc aaccacgttc
    18901 aattaataaa tatattccag aagaatggat caatgattat agtaaaaaaa tgttggcgac
    18961 gctttttatg ttactaagag gtaccccttt tatttatcaa ggacaagaac taggaatgac
    19021 caacatagaa atggcatcat tagaagattt tgatgatgta gctacgcatt ctcagtataa
    19081 acgggcatta gaatatggat tggcaccaga gcaagcattg aaagctgtca ataaacgaag
    19141 tcgggataat tctcgcacac ctatgcaatg gaatacgcaa aaaaatgcca gattctcaac
    19201 ttctgataat gtatggttaa aagtaaatcc taattatcca gaactcaatg cagaagctca
    19261 gttaaccaat caacagtcgg tattcaatta ttatcatcaa ttaattaaat taagaaaaaa
    19321 tagtgtatac aaagacgtgc taatctacgg acaatttcta ccagtgaaag ataaaaatga
    19381 acatattttg ctttatgaac gccaattaga agataaaatc gtattagtta tcttaaacgt
    19441 tacagcaaca gaacaagaat attatgtaga tgaacagtac caacaagtat taatcaataa
    19501 ttatgaagat gttttattag atcaaagtcg aaaattgcgt ttacgaccgt ttgaaagtat
    19561 tgtattagct aattatggag gacaattatg aaaattaaaa acgaagcaat gctcatcacc
    19621 tatgcagata gtttaggaaa aaacatgggc gaattaaaac aagtcttaga caaacattta
    19681 caaggtgtta ttggtggtgt acatctgttg cctttttttc catcgacagg tgatcgaggc
    19741 tttgcgccga gtgattatac gacagttgat ccagctcttg gaacatggga agatgtggaa
    19801 gcattaggtg aagattatta tttgatgttt gattttatga ttaatcacat ttctcgtgaa
    19861 tcaaaatttt tccaagattt caaaaaaaac catgagcagt caccatacaa agaaatgttt
    19921 atccgtattc atgaattttt cccagaaaat cgaccaacac aagaagatat tgatttaatc
    19981 tacaagcgta aagacaaagc gccgtttcaa acggtagaat ttgcggatgg tacgacagaa
    20041 gaggtttgga atacctttgg ggaagaacaa attgatttag atgtgaccaa agaagttgtg
    20101 aaagaattta ttcgagaaac catcaaagat atggcgaacc atggctgttc tttaattcga
    20161 ttagatgcgt ttgcttatgc gattaaaaaa ttagatacca atgatttctt cgtggaacca
    20221 gatatttggg aacttttaga cgaagtgcgt caagaagcag cgaaatatga cgtcgaatta
    20281 ttaccagaaa tccatgagca ttattccatt caaatgaaaa ttgccaatca tgattattat
    20341 atctatgact tcgccttgcc aatggtcact ctgtattcac tttatagtgg aaaatcagaa
    20401 cgtttagcca attggttgaa aatgagtccg atgaagcagt ttacaacatt ggatacgcat
    20461 gatggaattg gtgttgtgga cgctcgtgat ttattaacag atgaagaatt ggattacacc
    20521 tctgaggaat tgtataaagt cggggcgaat gtgaagaagg tctattcaag tgccaattac
    20581 aataatttag atatttatca aattaacagc acctattata gtgcattagg cgataatgac
    20641 cgtagctatc tgttagcacg tgcgattcaa gtatttgcgc cagggattcc acaaatttat
    20701 tacgttgggt tattagcagg aaaaaatgat attgaactgt tggaagcaac gaaagaaggg
    20761 cgtaatatta atcgtcatta ctatgattta gccgaaattg aagaagaagt gcaacggcca
    20821 gtgattcaac aattatttgc acttttaaaa tttagaaatg aatctgcggc gtttgatttg
    20881 gatggaacga ttgaagtagc aacgccatca gaatcagaaa tagtgattac acgtaaaaat
    20941 aaaaacggta gtgatagtgc aacattaacg gctaacttgg atacgaaaga atttatcatt
    21001 gagtctaaag atataaaaat tttcactagt gttgcattaa aattttaacc tattccatag
    21061 aacaaaaaat cctttatact agcacttgga tgacttatcc aagaccaata aaaaggactc
    21121 atacatggat aagtataaaa caaatacagc atttaataaa tggttttctg ccataaaatt
    21181 aggaaaactt tctgtcaact cccaaaaatg tatttttgat tacaattcgt atcgtaaaaa
    21241 actgtctttt gaagctgtgc tcaagctctt tctctacgca gttaatgaag aaaaagaaag
    21301 tttgcgtgat ttgagtacag ctcttatcga ccgatcgctc caactagaaa ccgatgtgtc
    21361 atcgattagt catacccaat tgtctcgagc atttaatgca cttgaaccta aagtgttaga
    21421 agaaattttt caacaactgt tagaaaaagt tcaacatcaa atcaaaccaa ccaagcgaaa
    21481 tcatctgtat ctgattgatt ccagtacgtt ttcgcttagt ttaaaacgtc ataagtgggc
    21541 gactttccgt caaacaaaat caggtgtcaa gttacactta aactattgtt atatggacga
    21601 tactacgatg tatccaactg attttacctt aacaaatgcc catgaacatg atctcaatca
    21661 actaccagta ttggtgaatc aaccagaggc aacttacgtg tttgatcgtg ggtattagga
    21721 ctttgaaaag atggatcaga tgcattggga tggctatttc ttcgtcactc ggattaaaaa
    21781 aaacaccaaa gttcacgtga tagatactct ggaaacatcg ccagaaacag aaattctacg
    21841 agacgagctg gttcgtttag gatctctaac cgccaatttc cgactagtca ctgttcaaga
    21901 taagaacgga cgtgtgtttc aatttatcac caatcgaatg gacgtctctt caaaagaaat
    21961 ctctgatatg tatcacgctc gttggcaaat cgaattgttt ttcaagcaca tcaaacaaca
    22021 catgaccatt aaaaccttct tttctcaaag tgaaaaaggc gtgcagaatc aattgatttt
    22081 aacgatgatt tccgctctcc tcacgtttct catcaaattg gagacgaaaa cggaaaaatc
    22141 agtgttccaa atcaaacgat tctttcgtta tttactgttt cagccctttg aatgttgtct
    22201 agaaaaatta attcccacat gagaaaaaag aaatttggct cgttactttt ggttttcagt
    22261 tgaataacag ataaaaaatt ttctggaaaa agtcatcctc tgcgtcagca cagttttttt
    22321 tgacttattt tcagaggaaa tagttttcag aatattctaa aatgttttat gcaacactag
    22381 tgatattttt tagaattaga tttctaatgt gcattttttg agtattaagg agcaagtata
    22441 caaagattta taaagctgat taaatttgaa taaaaatatg caggaacgct actattttta
    22501 gtagtgttct tttttatatt tgaataaaaa atgtggtggt taaatggtgg ttataacaaa
    22561 aaatgaatta atgaaattag gttatgggaa gtatcagtct gagagaatca tacgacaggc
    22621 aaaagcaatt atggtttcaa aaagctatac ctattacgaa aatagaagat taggactagt
    22681 acctgttgaa tctgtagaag aagttttagg tatgaagata gaggtagagg aggattttca
    22741 gaatcatggt taattttaaa aaagataaaa atgggaaata tcattttact gctagtttgg
    22801 gattcgacaa acgaacaggg aaaagagtac aaaaaaacgt agaggggtta aaacagttaa
    22861 agaagaaaaa aagcgtatac cgaacttgtt tctaattatg gaaaagaagc agaaacttct
    22921 caaagtacta tgttgtttga ggagtttttt tataaattat tcctacctta ttatcaaggg
    22981 aaagtagaag aaagaacata taaaaatcgg ttatctacaa tcaaaaaaac ttttcttatt
    23041 tctttaaaat ggagttaaga gaaatacgtc caattgacat tcaaaagtgg caaaataatt
    23101 tattgagcaa atataaaaat tcttatgtaa gaaacatgta tgggattttt cagatgtcat
    23161 tagatcgagc agttgttttg ggactacttg aaaaaaatcc tgcaaaagtt attggaaatg
    23221 tgaagaaaag gaaaactaca gttgattttt ggacaaaaga agaatttaca aaagttattt
    23281 caacttttta tattgatgat ttgtaccaac attttggttt tatttgtatg tggttacttt
    23341 ttatgaccgt tatgcgtttg ggagaagcca ctgcattaac ttggaaagat attgattttg
    23401 aaaataaaaa cttgcgtgtg aataaaacac tctattataa gagagccaaa acctatgagt
    23461 ttgtagaacc cgaaacggct tctagtattc gtacaatcga actagacgac aatacaatta
    23521 cctttttaaa agcttggaag gagcgtcaag tttcaatagg aggcatgtcg tttgtacttt
    23581 cttataatgg aacacctact cagaaatata caatccgtca tattatgaga agacatgcaa
    23641 cgatcgttgg tgttcatata atccgcatac acggattaag gcatagtcat gcttctttgc
    23701 ttatatcaca ataaaaagat gcacttcgaa ttaaagaaag gttaaaacat gctgatgtac
    23761 aaacgacttt aggaacttat ggtcatttgt atctaaattc attgagagag ataacaaatg
    23821 atcccacaga ttatatccaa actaattttt ctaaaatcaa accgacacct atttacgata
    23881 ataaaaaata aatgataaac aaaagtagga tcacaccaaa agaaacacac tagaaaaact
    23941 ataatacagg cttttctagc gtgttgtttc ttatttccgc ctatttatac attatactca
    24001 taaagcattt aatgcatcga tcgtagccgc attgtctata tgggtatata aagttgttgt
    24061 attggtactt gaatgtccca attgttgggc agttaatacc aatgaatttg tctgttggta
    24121 gagtctagta gctaatgtat gacgcagttt gtgagggctg acttgaattc taaagccttt
    24181 tgaatatttt gctaccatcc gttcgattgc aataccggat atacgcttgg cttctccttg
    24241 gtaaattgct agaaataaag ctttttcgtt ttctgttact ttatatcgtt ctttccgaat
    24301 agctagataa ttagctaaat attccattgc gaatgctgct atattgacag catctctttt
    24361 atttcctttt ctagttacta ccacattatt cttatctaaa gataggtcat ctaaattaat
    24421 atttataacc tcggagagtc tcaatccaga tcctaaaatc agtgcattga tcgccaggtc
    24481 tctttctaca tctcgttgat aatagatcaa agctcgttta ctcaaagtat ttttatattc
    24541 attggcaata taatctagat aattttgtgt atccttatct agaaataatt ttttctcaat
    24601 ggcattggca cgtgcagcta acgtagtttt atctgtgatg gttgctactt gatccatagg
    24661 attattgtct aagtatggtt ttcctagagg attattggaa cttttcgaaa gatatttaaa
    24721 tagaaccttt aatgccgtga tatttctatt gatagtagaa gtagatagac ctaagttatt
    24781 attatctttg agcttagttt ttgttgtctc tttttttaca ttttcttttg tgcgacgttt
    24841 caaatgagat ttgtacaagt ttaggtcata ggcagttaac tgtcttatcg aggtgataga
    24901 agtatcagga acgatagact cactcagaag ccattcataa aagagcttat aattttttag
    24961 atattctaaa gtggttcgag gtgattgatc tttatcctca cgatcttgaa caaaatcttt
    25021 tatataatcg ggcatatgaa gtatgattgt atctattttt tgatatattt ctgtattttt
    25081 catgtcattc atcctttcaa taagtagggg agaagagtat ctctataatt ggactatacc
    25141 aattatagaa tgttttgtca attggtatgc tctaaaattt attaatttgt ttcaaaaaaa
    25201 gcatataaac aggtatacgt ttaataattt aaaataaatg gtatacataa atagggaagg
    25261 aaatagaagg gagcaatcta aattttttga attttcttaa ttaattatat agaaagaaca
    25321 atctttctat agagttagac atgagaggga ctatgcttga tttaaaatca acaagcgtat
    25381 atactgtata tacaaagaaa aaggagggag aagactatgg caattaaaga aagaaaattt
    25441 agggaagtgg ggaactcagt agtattaact atttcaaagg agtttcttaa agaaaatggg
    25501 ttaaatcccg gagatacggt ttttttagat gaagaaaaac ttcgagcggc aattacaaaa
    25561 aaacaagcct tatctgatga aattgattta gcaatatctc aagcattaac tgaatatgat
    25621 actactttta gagagcttgt cgataaatga gtattcatta cctagaagcc aaagatatta
    25681 taaaaatgaa tgtaatccaa ataaaaaaat actctcctaa tgaacccctt ggtgtaaagg
    25741 atgcgaatgc tttagaaatg tgtgtagggc aacttcgagc ctcagctttt ggtgaagagg
    25801 tttatccttc gatttatgaa aaagcagcta tcctacttat acaattgata aagaaacatc
    25861 cttttcacaa tgcaaataaa cgtactgctt tcttagctac ttttgtcttt ttgaaaatca
    25921 atggacagct tttagctatt gatcaaaaag atgctgtaaa tttagcggta tatattgcta
    25981 cgtatgataa ggatttcgat caactaaaat atgaagtgat caacactata aagacacata
    26041 cgcagcccat ttaaggagtt actgtatgtg tctttatagg gttattagct gctatagcga
    26101 aataattgaa gtaaattaca aatagctata attcaaatcc aactatatag aaaacgtaaa
    26161 ttttctatat acttggatat gctggaatgt ttaagagaat aattacattt ctagaaaata
    26221 caataaaaat tacaggataa ttagccatat cctacctcct ctagcagttg ctcaaaagtt
    26281 agcactaagt aacttctttt ttgttgctgg taacaattta tcaatactaa aatagcgttt
    26341 aagtatctaa taaatactaa ttttaaagct cttaattatt atgaaacacg atataacaat
    26401 attgctatat cgtgctattg taatattata catttgaaga cagcgtattc ttgttctaat
    26461 atcaggaatt ttttataaat atcagtcata ttaagactca aaatttattg gtatgttcct
    26521 ctgtatagag tttgaccgtc taccattata tagcaattat tatcattgtt gtcttgcacc
    26581 aatataccgt taagggtagc actaggagta gtaacaatca ctggaaactc atgagaaagc
    26641 tcggtcattt ttcctgttct gctagaatcg tgttctattg tcaagccatt tactataaac
    26701 gtgaatactt tacttcctat tgttatagta gctttttgag aaacagtttc tgtagctgga
    26761 gctgtgcttc tttccgaatt tgttagggtt tcactggatt cattgctata aacaattgta
    26821 gaggattcta cggcttcttg agtgtccacg ttggtgctgc tagcttcatc tagcttcctt
    26881 ttgtattgtt cgttactgat tgctaagctt tcccgttggt catcaagctt attcatttct
    26941 gtaatggtat aaaataatcc tcctgctagt gctatattaa caactaaact aaccgcaaat
    27001 aatacctttt tcattcttcc ttctcttctt tctctttttg taattcctta cttttggata
    27061 ggctacaatt aactagacag aaaaattaag gtgtgtagac tagaagaaaa cataccagga
    27121 ggaattttta tgtctaagag aacacgaaga actttttcac aagaattcaa gcaacaaatc
    27181 gtcaatcttt acttagatgg aaagccacgt gtagaaatca ttcgagaata tgaactaacg
    27241 gcttcagcat ttgacaaatg ggtaaagcaa tctaaaacga gtggttcatt caaagaaaaa
    27301 gataatctta cgcctgaaca aaaagaattg ttagaactac gtaaaagaaa ccagcaatta
    27361 gaaatggaaa atgatatttt aaagcaagca gcgctgatat tcggacgaag agacaagtaa
    27421 tcgatgcgaa taagcatctt taccctatat cagcgatgtg cagaatatta ggtctatcac
    27481 gtcagtccta ttattat
//
LOCUS       DASHFF010000032        27493 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.032, whole genome shotgun sequence.
ACCESSION   DASHFF010000032 DASHFF010000000
VERSION     DASHFF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.032"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(51..1517)
                     /locus_tag="U2I75_001546"
     CDS             complement(51..1517)
                     /locus_tag="U2I75_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN1696687.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1903..2499
                     /locus_tag="U2I75_001547"
     CDS             1903..2499
                     /locus_tag="U2I75_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1696688.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2557..3039)
                     /locus_tag="U2I75_001548"
     CDS             complement(2557..3039)
                     /locus_tag="U2I75_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696689.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3145..3342)
                     /locus_tag="U2I75_001549"
     CDS             complement(3145..3342)
                     /locus_tag="U2I75_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696690.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3637..4395
                     /locus_tag="U2I75_001550"
     CDS             3637..4395
                     /locus_tag="U2I75_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN1696691.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4455..4883
                     /locus_tag="U2I75_001551"
     CDS             4455..4883
                     /locus_tag="U2I75_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696692.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4929..5001
                     /locus_tag="U2I75_001552"
     tRNA            4929..5001
                     /locus_tag="U2I75_001552"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4962..4964,aa:Gln,seq:ctg)
     gene            complement(5151..5522)
                     /locus_tag="U2I75_001553"
     CDS             complement(5151..5522)
                     /locus_tag="U2I75_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1696693.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5737..5922
                     /locus_tag="U2I75_001554"
     CDS             5737..5922
                     /locus_tag="U2I75_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696694.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6005..6193)
                     /locus_tag="U2I75_001555"
     CDS             complement(6005..6193)
                     /locus_tag="U2I75_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696695.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6217..6480)
                     /locus_tag="U2I75_001556"
     CDS             complement(6217..6480)
                     /locus_tag="U2I75_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696696.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6638..6895
                     /locus_tag="U2I75_001557"
     CDS             6638..6895
                     /locus_tag="U2I75_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN1696697.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6966..7157)
                     /locus_tag="U2I75_001558"
     CDS             complement(6966..7157)
                     /locus_tag="U2I75_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696698.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7298..7618)
                     /locus_tag="U2I75_001559"
     CDS             complement(7298..7618)
                     /locus_tag="U2I75_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696699.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7641..8075)
                     /locus_tag="U2I75_001560"
     CDS             complement(7641..8075)
                     /locus_tag="U2I75_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1696700.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8410..9048
                     /locus_tag="U2I75_001561"
     CDS             8410..9048
                     /locus_tag="U2I75_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1696701.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9372..9455)
                     /locus_tag="U2I75_001562"
     tRNA            complement(9372..9455)
                     /locus_tag="U2I75_001562"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9419..9421),aa:Leu,seq:cag)
     gene            9983..10483
                     /locus_tag="U2I75_001563"
     CDS             9983..10483
                     /locus_tag="U2I75_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1696702.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10673..11017)
                     /locus_tag="U2I75_001564"
     CDS             complement(10673..11017)
                     /locus_tag="U2I75_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN1696703.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11171..12091)
                     /locus_tag="U2I75_001565"
     CDS             complement(11171..12091)
                     /locus_tag="U2I75_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1696704.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12278..12769
                     /locus_tag="U2I75_001566"
     CDS             12278..12769
                     /locus_tag="U2I75_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN1696705.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12793..15168)
                     /locus_tag="U2I75_001567"
     CDS             complement(12793..15168)
                     /locus_tag="U2I75_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN1696706.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15494..16120
                     /locus_tag="U2I75_001568"
     CDS             15494..16120
                     /locus_tag="U2I75_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1696707.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16143..16847
                     /locus_tag="U2I75_001569"
     CDS             16143..16847
                     /locus_tag="U2I75_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1696708.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17039..17872
                     /locus_tag="U2I75_001570"
     CDS             17039..17872
                     /locus_tag="U2I75_001570"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN1696709.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            18069..19997
                     /locus_tag="U2I75_001571"
     CDS             18069..19997
                     /locus_tag="U2I75_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1696710.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            20135..20590
                     /locus_tag="U2I75_001572"
     CDS             20135..20590
                     /locus_tag="U2I75_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN1696711.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            21060..21329
                     /locus_tag="U2I75_001573"
     CDS             21060..21329
                     /locus_tag="U2I75_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696712.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            21729..21983
                     /locus_tag="U2I75_001574"
     CDS             21729..21983
                     /locus_tag="U2I75_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN1696713.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(22072..23118)
                     /gene="holA"
                     /locus_tag="U2I75_001575"
     CDS             complement(22072..23118)
                     /gene="holA"
                     /locus_tag="U2I75_001575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN1696714.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(23156..25429)
                     /locus_tag="U2I75_001576"
     CDS             complement(23156..25429)
                     /locus_tag="U2I75_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN1696715.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(25422..25961)
                     /locus_tag="U2I75_001577"
     CDS             complement(25422..25961)
                     /locus_tag="U2I75_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN1696716.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(26097..26315)
                     /locus_tag="U2I75_001578"
     CDS             complement(26097..26315)
                     /locus_tag="U2I75_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN1696717.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(26312..26557)
                     /locus_tag="U2I75_001579"
     CDS             complement(26312..26557)
                     /locus_tag="U2I75_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1696718.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(26673..27401)
                     /locus_tag="U2I75_001580"
     CDS             complement(26673..27401)
                     /locus_tag="U2I75_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN1696719.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         8527 a         5353 c         4680 g         8933 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaagaag atagagattt aataattata
     1681 tatcagctgt ttttttcaaa taagcaaaaa ttcctaaaac acaacaatga aagcgataac
     1741 taagaaaatc cttacataac gatttttctt attaaaattg ttttttgaac ttcgcttatt
     1801 ttttgtgagc atatctgttt acttactttt tgtaaggtgc tatacttcat ttcgtaatca
     1861 attaaaaaaa tgcaaaacgc aaatcaaagg agaaatttta ttatgacaac ttttactgac
     1921 actttgaaaa accgccgttc aatctatcat ctaggacgta atgtttcact ttctaatgaa
     1981 gaattaacaa ctttgatcaa agaagcaatt aaagaaagcc caactgcttt caatgctcaa
     2041 tcaacacgcg cagttatcct tttcggagat gcacatgaaa aactttggga aatcactgaa
     2101 gaagcacttc gcccattgac tccagcagaa gctttcccaa atacacaaaa caaattagct
     2161 ggtttcaaaa atggttatgg tacagtatta ttcttcaaag atactgacgt ggtaaaaggt
     2221 ctacaagaac aatttgaatt atatgcagac aacttcccag attggtcaga acaatcaaac
     2281 ggaatcgcaa cagctaatac ttgggtagca ttagttgata aaggattggg cgcaaacttg
     2341 caacattaca acccagttat tgacgaagca gttgcaaaag aatggaatat cccttcaaac
     2401 tggaaactac gttcgcaatt agtattcggt tcaccagaaa caccggctgg ggaaaaagaa
     2461 tacatgaacg atgccgatcg cttccgcgta tttggataat tttatttgaa ataaaaaaca
     2521 gaggttgtga caaacatctt gtcgcaacct ctgtttttat tctggatcaa tcataatatc
     2581 aatctctgcc tcatcgttta agcgttggga caaaaatgga actaattctt ctaatgatgc
     2641 agaaaatttc agcatcggtc ctcgccatcc gtttcggaaa aaattcattt ttacgatttt
     2701 agaacctttt ggctgatata atacaatttt attgatctca gaaaaaggaa cacctttttt
     2761 gtcaaagcta ttcaaaacaa gtccttcttc tgtgattcct ctactgtcca acaaaaaact
     2821 aataaaaatc attgctgaca agatcccttt tgccaattca tctgctgtag gatgactgat
     2881 gagtgtatac caaatgacta gtgacactaa gaagggtatc accaagtaga cccatcgatt
     2941 tcgcacagtc cgataaataa tttcttttcg aaccagccaa agaaataaaa acatcacaac
     3001 aacgaataaa aacaatagaa ccgtaaagaa tgttaccatg acaactcgcc tcgctttttc
     3061 ttctccagaa aataggaaaa gaatactgat ttaccttctc aaagaaattg gacttgcagc
     3121 acgccgcacg tccaattgtt ttctttattc aaatgtatca tattttgcaa caaccatgct
     3181 tgctttttca tctaataatt cttggtcctc tgcagcacat tgggcaactt tgaccacggc
     3241 gcagtttgtc atttttgata caacttcacc aatgaccgtt tttgtaaaac caattggttt
     3301 gcatgcatat tgttctccta aattaatcat ttcttccgtc atcaaaatcc tcctaaactg
     3361 ctggaacttt tctccacatt accactgcta tttctttgga gatttaactc attgattttt
     3421 atttacagtt aaaaatcaac cttacaggca ttatagcgta ctgttttcgt tttcacaatt
     3481 aaaacagctg ttgtatttag aagaattttt ttcaaaaaat taaaacatat tccttaaaaa
     3541 ctaattattt cctacatatt aaaatataat acttccattt ccaataaaaa tacatttata
     3601 ataggaaaga taaacagctg aaaagggaga tttcaaatga agaaaaaaac gttcaaaaaa
     3661 ctattgtttc tagggggcac agctgctctt ttattaagcg gtacaagtta ttacctacat
     3721 atccattctc cagaacatac aatgaacact gcacaaaaaa gttcaacaca aaaaacgaaa
     3781 aagacaaaag agaaagcaga agttttacta gatgttccat tagagagcca gtttgattct
     3841 ccttctttag aaaatggctg tgaagtcact tcgctttcta tgcttttatc tttttatggt
     3901 tatgaaacaa ctaaaaacca gttagctgag caattggatt acgtccctgt tttcaatgca
     3961 gatgggactc atggcgatcc taatgaagga tttgttggtg atatcagtgg cggagactgg
     4021 gctatggtcg tctatgttcc cccagttgcc tcccttgctc agcaaatcgt ccaaacggat
     4081 tatcacactt ttccaaaaac cgatgctcaa ctcgatgata tcaaaaaagc actatctaaa
     4141 ggaaatcctg tctggacttc tgtgactatc aattttgaag tcccagatga aactgatttt
     4201 atgacttgga ccacaaataa tggagaagta agagtcactc ctcttgttca tgcttgtgta
     4261 gtcactggat atgacaaaga aaatatttat gtcaacgatc cctatggtgt aaaaaatcga
     4321 gcagtaccga tcaacgattt ttcggcgatt ttcactgcaa tgggtggaca gatgctaact
     4381 ttggagaaaa gctagtaatt caaacaaaaa tcccttatac tcaagatacc aaacctttga
     4441 agggagcgta gataatggaa aaattcacac ataaaaaaat ggatccaaat gaaattccga
     4501 ttatttttgt ccgtgactgc aagggaaatg ttcaaggtaa agtctctatt aatgagtgga
     4561 acgagcgtcg cagacctgct accctcaatg aactggaaat caaattgtat cgtcaatctc
     4621 ttgtttatta cgcagatcaa gagtacgaga aagccaccga tttattgaaa tttctaattg
     4681 ctcgaacaga gtatactcgc tttgaatata ttgaacgttt agcaaacatc tatcatatta
     4741 tgaatgaacc tgtcaaagaa tatcagttat tggacaccgt cttgtcagta gcagaactga
     4801 ttgcccttcc tgctggatta gaaaagaaac tagtacgccg ccttttacga gtaaagcaac
     4861 aactatccga tcaagaaaaa taacttgcgt ttttttagga aagtgctatg attattttgt
     4921 tgaataaatg ggatgtagcc aaactggtaa ggcacttgac tctgaatcaa gcaattgtag
     4981 gttcgaaccc tgccatccca atttgatgtg cttgctgaaa aaatgaaccc cctgtaaaag
     5041 aacaatcttt ttgcaggggg attattgtat tatttttact acttgtattt agtttacata
     5101 attaaattat gaataataaa atcgggcgta ttcttaatac aaaaactgat ttacccaata
     5161 attgctaatg ctttctccgg aactttttct ctaggaactt cctcatacgt ttctatgtgt
     5221 gctcctttat tgctaatttt caaataatga ttttctttta acacagaaag accattaaat
     5281 tcaaccgttc gttctttccc attggcatca gcagctttct gcacatacct tgctgtacca
     5341 tattcattta cttcctctgg tttttgtgtc tttacataga cctctccttt ttcaactaat
     5401 ggatttagct gatcaagtat accagggatt tctccataac ttccttcagt ataatatttc
     5461 agtccaaata gcaaaatacc actaatcgcc gccagtccaa tcaaatattt aatcactttc
     5521 atctttctac ctccttgtta tatattcagt atatgggtca gttaaagaaa ttccatccgt
     5581 cccaagttgg agatttttct cctactttag aagcgagaat cgagtgtttt tttagtaaaa
     5641 atgatcgtta tttgatatta ttgacgtgtg gataaatcag tttttctcgt tttgtccatg
     5701 taaaaaatca tgttgatagc aaaggatgtg ttcaaaatga atcaagaaga attatttcaa
     5761 aaagtcaagg aaatgattaa gaacggcaat ttcgatggcg caaaacgatt tatcgaagaa
     5821 cataaagaac aattaggacc ttataaagag aaagcccaaa atcttttaaa agatgtaaat
     5881 atcgacagtg tcaaaaacaa atttaaaaat ctattcaaat aagtaaaact ttcacttttg
     5941 taaaatcaaa ggaggacaat gctgctgcat cgtcctcctt tggttctttt ctgaatactt
     6001 catactaaaa taaacgtgtc ggatcgtaat gataagtcac attgccgttc agtgaggctt
     6061 ttcccaccac atatttacga ttctccaact tcagccacgc ttctcgaaaa gccattaatt
     6121 cttctctttt tacatcaatc ttttcgattt ttccatcgat caaatcttga ataagttgtt
     6181 catatggatt catttttttc ctccatctgc tgtcaatcaa taagttgtaa aaataggttc
     6241 atatgctttt ttggtatcta gatcttctct gactttgact gtcgtataaa taattttttc
     6301 tactgggata ctataataac ttccttcacc attattgaat cggtgaacgt ctttctttgc
     6361 taaaatacca tttattttat tcgtcagtac acgttgttcg acactgttac ttacaacata
     6421 gtaaatcacc cgaatattat tgatcaaatg caaatcgatt tctaacacta catgatccat
     6481 gaaaatccca ctccttcttt cactttgatt atagcaagtt tctcctctaa tttcaaaatc
     6541 gatacagatt ttaccggata aatccacaaa actgcttgcg ccatttcgca caaagtgtta
     6601 ttttgtatca tggactttat ttatcggagg caatagtatg atcgcgatcg gtcaatttgt
     6661 tttttatatt ccttttttta ttatgataag cattcttttc tattacataa aatggacaaa
     6721 aaagaagttt tcagttttac tggcctcttt acctgctgta tattttactt atcaaatttt
     6781 ttcttttagg cattgggaaa caacgtctgt attaataaca cacatcatcg aattgacgct
     6841 ttcagtaatt tttcttatta tttggatcta ttttttatat aagaatcaga attgaatccc
     6901 tcgcatgttt aaaaagaaaa cgattaaaga tgcagcaaat ttctgccgat ctttaatcgt
     6961 tttttctatt ctgttatacc aaaaaaatgt ttgaatcggt gaaggatcgt tccttgtttt
     7021 aattctataa attcttgtgc gatcgggtac ttttcttcca aattttcttt gttcttttcg
     7081 atcaggcttt tcgcttcttc atatttttta tctgcacata gtgcttttac ttcctgtaat
     7141 acttttcctg cttccatttg cctcaccctc ttgccatttt attgctttaa gttttattta
     7201 agttatacgc attattctag tttcatccca aataaccttt tttcattttt actcctcgaa
     7261 ctaatcagtt ttctaaaggc tgattggttc gtctttttta tttttcctcc atttcattct
     7321 gctcttctgt taatttatct aatgctttga ttgatgctgc ggctcttgca taatcatagc
     7381 tgtataattc cataaattta tcaggcagaa caaaccacgc tggatgataa ccgttagctg
     7441 tgacagggaa tcctttatct tgttggttat ctccatccca tctcagagca acaccttttt
     7501 ttcctttcca ctttactaaa gcgatcgagt atcccttctt tgtttgtgtg ccgtcttcaa
     7561 taattgctaa aatttctatt ttttcttttg gcgcacggac ttcttgagga cgaaccattt
     7621 tctattcctc ccttatctca ttacttcacg accaacacac tgcaaggggc atgtgatacg
     7681 acataagaag cggtcgatcc aactaatgct tgttgtatag caccttttcc tgtcgctccc
     7741 ataacgatca aatcaatatt ttcttgtcca gggatgacat tggcaatcaa atttcttgga
     7801 tctccagttt caactattgc gtggatctca gcaatgccgt actctttggc atcattgatt
     7861 tttttcagca tggctacttc tgttctttgt ttttcatctg catagatttt tgaaaaggca
     7921 taggcactat gggtcagctc aactttgttg atgattgaca cgataaataa gtgggcttca
     7981 ttgtcctttg ctaagtcaag ggcttcagcg aacgctttga tcgattgtct tgatccgtca
     8041 actgctacca tgatgttctg gtatctttta ctcattggaa ccgcctcctt tttcttttat
     8101 tgtaacatct caagcaaaaa tacaaaaaat atacgcaata tgtaaataaa atgatgaaca
     8161 aaagcaattt ctttcaagtt atttatacat ttcttttctc atcagaacac ctgttctaat
     8221 cccattattt ctgcattatg tgaaattcct catcattttt cacatcgagc gaacatcatt
     8281 ctgttacaaa aaacgtggat ttgtcataaa ttgtcatgct tcttttacct atgaaatatt
     8341 ttcacgtgtt aaagtattcc tcgttgaaca caacaaataa aactttttaa cgaataaagg
     8401 agatttttca tgacatcact taaaacttta ctttttggaa caactttggc tgccggcgct
     8461 gcattcttca tgggaacgac tgctcatgcg gacgaagctt ataccgttca atcaggtgat
     8521 actctatcaa cgatttctca aaaatacgtt ggtgataact ccttgatcaa tgccattgca
     8581 gaatcaaatt caatttctga tattaacctg atctactcag gacaacaatt aacaatccca
     8641 acagaaggct ctgcacaagc agctgctgaa cctcaagcag ccgttcaaga agcaccagtc
     8701 caagctgaac ctgttcaagc tgaacaacct gttgtacaag aaacggttca aacggaaaca
     8761 caagcagcgc ctgttgctga aacacaacct gctcctgctg taacagaaac tgctgctact
     8821 ccagcaagta caagctctgc aaaagaatgg atcgcacaaa aagaatcaag cggttcttac
     8881 acagcaacga acggacgtta catcggccgt taccaattag attcttctta cttaaatggt
     8941 gactactctg ctgcaaacca agaaagagta gcagaacaat atgttacttc acgttatggt
     9001 tcttgggaag cggcgaaagc attctgggaa gcaaacggtt ggtactaaga ttcattattt
     9061 gaacgtttaa acacattcaa aatatagtaa gaggtcatgc gatttattct ttcgcatgac
     9121 ctctttttga gtttaagttt agtctttatt ccttaactat cgtttgccag tatttcttgt
     9181 atcagaatct ataaatctac tatttgaatc aatctattag gttttaaact gtcccacctt
     9241 cacacaaatt ttttttgatt tatttgaatc tggttccgtt gtttaaaatt tttcctatat
     9301 tcttttagat aatatgttat tttctttctc atcgctccag gtgtaccttc tctttataaa
     9361 aaaacaaatt atgcggctaa gaagactcga acttccacga ggtcaccctc actagcgcct
     9421 gaagctagcg cgtctgccaa ttccgccata gccgcatcac atcttttagt ttaggattac
     9481 atgtgttttt tgtcaagaac atatttttgt tctttttcat ttctgtacgc cctcccttat
     9541 gccatctaca caaataattc atccgagcaa ctccatcatc tattgagggt tggtgaaaaa
     9601 atctgtttta tccatatgat cgatttcaat gttaaacgaa cgactctggg attagtaact
     9661 attttatttt tttcttcatt tcttttttcc tctttttctc gattcagcta tcttctttca
     9721 tttggatatt gattatttct tctgttgatt actatccttc tggaattcta gacgctgaag
     9781 taattcacgt tttctttctt ctagagatca taagtaacga atgaacgaac ttcaaataat
     9841 ttttttattg taattcatat ttgtattgtt cgatgattct taatacgata tgacacaata
     9901 aataaaaata ggcatggcta tttacaacaa tagtgctata gtagaaaaca aatgaataag
     9961 gttacttaaa gaggtgaata cgatgaaact gatcaaacta tgggaagcag atttaaagaa
    10021 agcttatcag ctacaaacta gctttggacc aaatgagagt ggatttatca acaatgctta
    10081 tggctataca tttgctgaat ttttagatta tgtaaaaaaa tgcgaaaaaa cgtctttagg
    10141 aatcgatctt cctgagagtt acgttcccgc aaccgtcttt attttagaaa gtgatgaaca
    10201 tgagtatgta ggaattttta atttgcgcca taaactgaac gaaacattaa gaaacggcgc
    10261 tggccatatt ggttacggca tctcccctaa ttatcgtaaa aaaggatatg ccacacaagg
    10321 attagcatta gtactacaag aggcaaaaaa aatcggcatt gatgaggcac ttttgtctgt
    10381 aaataaaaaa aatcttggca gtcttaaagc acaacaaaaa aatggggctg cgatttatcg
    10441 tgaagatgag aaagaatact atacgaaaat caaactgatg taaaaaagaa ggattccaaa
    10501 aggaaaggga tttgaatgtc ctttatcaat agaacacatt tatttaatta acttataata
    10561 tagcagacaa aattttataa acaaagaaac cttttatctc ttttcttcta gaaaggataa
    10621 aaggcttttt tgtttttcct atttgcttct catagctagt cagccaagat ttctatcgta
    10681 tccccaacgc aatccgtgca taacggctca tcttatctgt cgtccatgct ggataccaga
    10741 ctagtttgac ttctgctttc gttacttctg gcacttcttt taatgcgtca tggatagatt
    10801 ctgttaaaat atctgctaat ggacagccca tagttgttag agtcatctta atgactgttt
    10861 ctccattttc aggattgaat tctatatcat aaatcaagcc taaattgacg atgtcgattc
    10921 ctaattcagg atcaatcacc atttccaagg cagcaagaat acgttctttg attagttcta
    10981 tttcttgtcc agaccgttct tggtttgctt cgctcatcgt acgacactcc ttaacacgat
    11041 ttttatttat catactcttt ttttagctgc ttgtaaatga gaatttagaa gtaagattct
    11101 cgtttaaatt tataaaaatg ttcttattag atgtaataga ttaaatttat tctaaaataa
    11161 acctgattcc tcattttttt tgtgaacgat tttttatttc tggcaatacg ccagttgttc
    11221 gatacatttt ttgttcgttt tcatttgcaa accggccaaa tactgaatga gttggtaaaa
    11281 tattgataaa tgcatcgtcg tcgatttcat gaacgatctg atcaagatca tacatttcgt
    11341 agcgggtgat gaccatcatg atcatgttcc cttgtttttt tgaatagcca ccgacagacg
    11401 gcagtaatgt cattccacgt accatccgtt ttgctagttc ttctgttacc tcttctggct
    11461 tgacggtaac gatcattgct gttacttttt gatgactcgt gtggatggca tcaattactt
    11521 gagtcattgc ataaatggaa ataatcgtat acaaggcact ttcccagtta aagaagaagc
    11581 ctgcgatgac tacaatgata ccatttagaa tcagcatgta gtttccgact gtttttccag
    11641 ttgtttgtga aagtacaaga gagatgatat ccatcccacc agttgtaaag cccattttta
    11701 aagataatcc tacgccaaga cctaataaaa caccgcctac tagggcattc atcaaaatat
    11761 tatcagtgat ctgtccgact ggaagcagca tcgtcatcac cgatacacat acaacattca
    11821 caaaactaag gatcgttgct cgttttccta ctttgagaaa ccctaataca aacacaggga
    11881 tattcaaaag aaaaataaat gttcctgtgt ctatcttgat atgaaaccat tcatataaaa
    11941 gcgaggaaat gatttgagcg atcccattca tccctgcaga aaagacattg gctggaatca
    12001 aaaaataatt taaagcgacc gcagcggtca atccggttat caaaataacc actattttct
    12061 ttaatctttc atttttatga tacgcagtca taattttact cacaagcacc tctcccctac
    12121 tttggtattc caaatgtaag aataacagtt tatctattaa aataaaatac ttcgaactca
    12181 aatgagaaga attttagcaa acaggtaaga atctgcccta atcctatgat aagatagatg
    12241 tatggtaaat atttatatgt atatcaggag ggttcctatg gcaaaagatg ctagcttcga
    12301 cattgtatca gaagtcaata cagaagaagt aaaaaatgcg attcagcaaa caacaaaaga
    12361 attgtccaat cgctttgact tcaaggattc tacagtagaa atcaaattag agaacagcaa
    12421 tctagttgta ttgagcgatg atgatttcaa aatcgaacag atcaaagatg tattatttgg
    12481 taagctgatc aaaagaaatg tgccaatcaa aaatatccat ttttccgaat cagaacacgc
    12541 tctaggtggc aaagctcgac aaactgccga tttggtcaac ggaatcgatc gtgaaaacgc
    12601 gaagaaaatc actacagcta tcaaaaatga aaaactgaaa gtaaaagcac aaatccaaga
    12661 agatcagatt agagtgacag gtaagaaccg tgatgatcta caagctgtga ttgctttatt
    12721 acgtaaactt gacttaccaa tcgagttgca attcactaac tatcgttaat caaaaaacga
    12781 ggctgacgtc tgtcattcct cgtttttttg atttactgct tcttttgata accaaaaacg
    12841 ctgtattcct tcttccaatt ccccaacact tcgtacttct cttgctgttt gccaatgttg
    12901 tgggaataaa cgtcgataag aaaaactaga aaatcgttga tctgccaata caacgattcc
    12961 ttgatccgcc atgccacgaa tcactcgacc accagcttgc aagactttgt tcattccagg
    13021 aagctgataa gcgtaagcaa atccaaggtg ttccttctca tcataatatg atttgatcag
    13081 ttcctgctca tgattcatct gcggcaaacc tacacctacg atcatactgc cgatcaatcg
    13141 gtcccctctc aaatcgattc cttctgaaaa aatcccgcca agtacgcaaa atccaatcaa
    13201 tgtttcagcg ggatttgttt gaaattttgt taaaaatcgt tcgcgttctt cctcattcat
    13261 gtccgtttct tggattagca cattgctgtc gggatattgc tgtctgaatg cttccaccgc
    13321 aagatctaaa tattgataag aaggaaagaa aacgaaataa ttcccgacct ttccatcagc
    13381 aaaagcatga atcgtctcta ctaattttgc cagactgtgt tccctttttc gataagtcgt
    13441 ctctaggtaa tttgcgatga aaacttgctg gtttccttct gggaaaggac tcggcagttt
    13501 gtaagccaga cttttttgtc cccccagtgt ttcctgataa taagagagtg gtgaaaaact
    13561 agcagaaaaa agaatactgc tttttccttt atccaacgac tgctcaagaa acaagcttgg
    13621 atcaatacaa aactctttta ctatcagatc gcgatacgtt ttttcgactg ttgtttcgta
    13681 gtgatcatca taaaattcac tgaccttcaa aaaatgaagc aaatcaaaat aataaggaag
    13741 caattgttct tgtgatgggt gctcaggaaa ttctgccagc cattctttaa ttttttcgct
    13801 taattgataa cctgccttta ctaaagattc agccggtgct ttttgatgat gataatccca
    13861 atgatcttct tttgctattt ctcggatcga atcaaattct tttaatagtt tgttgaaacg
    13921 gcgatgaagt tttttgaatt cctttggtat agcttcctta cttcgtttcg ttttttcata
    13981 agatagttct gccgaataca tctctcggga acgattcacc aaattatgtg cttcgtcaat
    14041 caaaaacaga tagtcctcgt ttttttcatc ttcaaaaaaa cgtcttaaat aaacagttgg
    14101 atcaaataaa taattgtaat ccccaatgat tacgtcacac cagatactga catctaatga
    14161 tagttcgaaa ggacaaagtg tatgctttct tgcataagtt tcgatgactt cacgagtgat
    14221 ctgattctca tgattcaaaa gatcccataa accttcatta atccgattgt aataaccatt
    14281 ggcatatggg cactgatctg gattacaggt cgtctcatct aaaaagcaga tcttgtcttt
    14341 cgccgtcaaa gtgacacttt ttacttcagc tcctgtatct tttaatgctg tcatagcatc
    14401 ttcagctact tgtctagtta ttgttttagc cgttagataa aagatctttt ctccctcttc
    14461 ttcccctaca gctttcaaag cggggaataa cgtagagatt gtttttcctg ttccggtagg
    14521 tgcttctaca aacaatttct gtttcgtacg gatcgtttta taagcagcta ccgctagttc
    14581 acgctgacct ttcctatact catcatgggg gaatttcaaa gccatcaaag aggcattgcg
    14641 tacagtgcgc cagttttctt gaaaaacaag ccattcttta taatcttcag tcagcttttt
    14701 aaaaaaagtt tctaactgct ctcttgtctg atgttcaacc tttctcgtga tcacttcttc
    14761 tgccgtctgg aagtaagtca attgcagatt tatttcttgt aaatcatgtt cttgactata
    14821 gatataggca tacacccttg cttgatgaaa gaaaagttcc acttgttctg gttctaaatc
    14881 ttcaaatcgt ggttcggagg ttttgatttc atctatataa taaataccgt ctcttttgaa
    14941 aataccgtct gcacggcctt caacgatcag gtaatattca tctaattgaa ctgttgtttt
    15001 taaaaagact tctttttcat agttttctcc cgcatctttt tggagcttgc ggtggattct
    15061 cgctccttct aatgcagtat ggttactttt tttacgattg tcgatacttc cacgtcgcaa
    15121 aataaattct actaagtgcc gcacagatat ttttgactgt ttgatcattt ccaccacctc
    15181 tttctctcta agattacctg aaaaaaccta gtttagcaaa aagagctctc tttccaaaca
    15241 taaaaaaaca ccctgctgat taggcaggga tataggatat agaatttgga tttttggatg
    15301 aatctaatgt aacaaggaca tattaaaatg ctatgaaaac ttactttcct tctctttaga
    15361 atatccaaat acttttcata tagtctgata attcagataa gtaaagagaa ataagtacct
    15421 gtttttgttt cctagcaatg aatgcgttat actaatggat gaaagaagtt ttggctatca
    15481 aggaggaatg cacatgcata tcgaaagatt aaaaacagga atgattgaag aaaactgtta
    15541 tttggtctac aacgaagaag cacttttgat cattgaccct ggagaagatg cggaacgaat
    15601 caaacaagaa atcaaaaaaa caaaccaaca gccggtagcg attttgctta cccatactca
    15661 ctacgatcat atcggtgcag tggaaccttt acgcgcgtat tatcaaatcc cagtttatgt
    15721 cagcccacta gagcaaaaat ggctaggtga tccgattttg aatctttctg gactcggtag
    15781 acatgatgat attgctgatg ttattgtctc gcctgcagaa tatgaatttg aaatgaaaac
    15841 ttatacttta ggaaacatgt cttttcgcgt cgtccctacc ccagggcatt ctataggaag
    15901 cgtcagcttt atttttgatg attttgtcgt ttctggcgac gccctgttta gaggaagtat
    15961 cggtagaacc gatctgtata cagggaattt agaacaatta cttcatagta ttcggaccca
    16021 gctgtttgta ttacctgatg aatttgcagt ctatccagga catggcgatg caacgacgat
    16081 tgaacaggaa aaaaggacaa atcctttttt caatcgttaa aacgacttag gaggagaata
    16141 aaatgacaga aactacactt tatatcgtac gacacggaaa aacgatgttc aatacaattg
    16201 aacgagtaca aggctggtgt gatacacccc tcacaaagca aggacaagaa ggcattcatt
    16261 atttaggtcg cgggttaaaa aatgtcgact tttcatttgc ttattctagt gacagtggtc
    16321 gtgcaatcga aactgcccgt attattctag gagaacattc taagggaaaa gaaattcctt
    16381 attttattga aaaaaggatt agagaatggt gtttcgggtc tcttgaaggt ggatatgaca
    16441 tggaaatgtg gggtgtgatc cctcgtgtat tgaattttaa aacatatgat gaaatgttta
    16501 ccacagaagt ttcttttgaa caaattgcta atgctgtata caacttagat accgctgggt
    16561 gggcggaaac ttatgaaaca ttgcgagacc gtgtatggac aggatttgaa gatatcgccc
    16621 atcactgcga aaaaaatggt ggtggaaatg ttttagtcgt atctcatggt ttgacgatcg
    16681 cctttttgct taatctgatc gatcctaaac agcctgttcg tgctgggctt ttaaatggca
    16741 gcgtaacaaa ggtcacttat gcaaacggtc gattttctat ccacggcatc aatgatacgt
    16801 cttatatcga taacgggaaa acacatagtc gcgactcgtt tctataatgg ggatatttta
    16861 atttttacca gagaatcata aaaggcaaag tctgaacgag ggctttgtct tcttttttct
    16921 cttcgtgtac ttatctgctt tatttctcaa gtttttctta atattaaaaa acagattggg
    16981 tttctcaaaa aataacggta tactataaga tgtatcttaa ttgggaagga aggaatttat
    17041 gttttttgca aatattatca aagcagtgat tctcggtatt attgaaggaa taacggaatg
    17101 gctgcccatc agtagtactg gtcatttgat cctagcagat gaattcatca aactggatgc
    17161 cagttcacaa tttatgtcga tgttcaatgt cgttatccaa ttaggggcga tcttggctgt
    17221 cgtcgttctt tattttcaca agttaaaccc attttctcca agtaaatcaa cggtagaaaa
    17281 aaaagatacg tggacacttt ggtttaaagt gatcgttgct tgcataccag ccatcatcat
    17341 tggtttgcct ttggatgatt ggttagaagc aaatttccac aagttcttac cagttgccat
    17401 tgttttgatc gtttacggta ttgcctttat tattgtcgaa aaacgcaaca aaaacaaaac
    17461 acctcgttgg acttctctga atgactttac gtaccaagca gctcttcttg ttgggttatt
    17521 ccaagtcttg gcacttgttc ccggaacatc ccgttctggc gcaacgatcc taggatctat
    17581 cttgattggt gcttcgcgtt ttgtcgctac agagttttca tttttcttag ggattcctgt
    17641 catgtttggc gcaagcggct tgaagatcgt caaatattta gctgaaggga attcattcaa
    17701 aatggaagaa actgttgttc ttcttgtagg aacattcgta tcttttgtgg tttccgtctt
    17761 agctatcaaa ttcttactaa gctatttgaa gaaaaacgac tttaccgctt ttggctggta
    17821 tcgtattatc cttggtatca ttttgattgg ttattggttt attgcaatgt agaaaaaacc
    17881 agaacacttc tcttatttga gaaatgttct ggttttttat tttctttaat aataatcatt
    17941 ataaataata aaaacaacat caagcttaat caccaaatga taaccattat cgttctcatt
    18001 tagctgttat tattatcaat tacattttta ttatgctatc cttgttttga caaggaggtc
    18061 taataacgat gactaatcag aaaaaagctt ttgcagcaac aatcggctgc cttttattta
    18121 tgatcttcgg ttttatcttc gaccgattct ctcttaccat caccccactt ttttatatca
    18181 gtgccattat catcggtgga tataaacaaa catcggaagg cttgaaagaa ttgtggaatg
    18241 atcggacact gaatgtggat ttattgatgg ctttagctgc tattggtgcc tgtttgatcg
    18301 gtaattactt tgaaggagca atgttgacat tcatcttttg tttaagcggt gcattagagg
    18361 aatacacaac taataaaagt caaaaagaaa ttactgcttt gatgaatctt caaccacaaa
    18421 aagcccaatt attaaaagaa aatggacaaa tgcaagaagt agatgtttct caactaaagg
    18481 tggataactt ggtatttgtc gctaaaggag cagctgttcc gatagatggc tttttagaaa
    18541 gcacacaggc aacgatcgat gagtctgcat taagcggtga atctgtgcct gtagaaaaat
    18601 atttaggcga tgagatgtat gcaggaacgc tgaatcaagg aaatcccatc acgattcgag
    18661 taacaaaaac gagttccgaa actgtctttg ctaagattat ccaattagta gaagaagcgc
    18721 agaatacgcc aactcaaaca gcttcattta tcgaacgaat cgaaaataat tatgtaaaat
    18781 tgattttgct tgctgttcct ctaatgatcc ttctccccca ctttttccta ggttggagtt
    18841 gggatgaaag cttttatcga ggaatggtat tattagttgt ggcttctcct tgtgcgcttg
    18901 tcgcatctgc tacaccagct tctctagctg ctctttcaaa ttctgcaaaa aatgggattt
    18961 taatcaaagg cggtatccat ttagaaaagc tttctgaatt aaaagcgatt gcctttgata
    19021 aaacaggaac gctcactaaa ggaaaaccag ttgtaactga cagctttttc tttgatgaaa
    19081 aggatttggc acaacaactt cttgttgcta tggaacaaaa aacgacgcat ccacttgctc
    19141 atgcgatttt gcaatatttc gattgcacaa tacctgaaga aatccaatac ttaccaatag
    19201 aagaaattac tggttttgga ttacaaacta cctatttagg tgcgcaatgg aaagttggca
    19261 agcacgcata tgatcctgag acgatgataa tcagcaaaga gattgcggaa atgatcgaac
    19321 gtttggaaaa tcaaggcaaa accgtgattt atctttctaa agatcagcag ttaattgctg
    19381 ttttaggtct gctagatatt ccaaaagcaa atacccaaca agtaatttcc tactttaaat
    19441 cccaaaacat ccatacaagt atgataaccg gcgaccattc tggcacagca aaggccattg
    19501 ctgaacaagt agggattgaa cattactatg caagctgtac tccggaagaa aaaacacaac
    19561 tagtcaaaaa agagaatgaa ctttaccaag taaatgcgat ggttggtgat ggagtcaatg
    19621 atgcgcctgc tttagcagcc gcttctttag gtgtcgctat ggggcaaggg acagatgccg
    19681 ctatggatat cgcagacatc gtcttaatga aaagcgactt aaataaattt gtctacagtc
    19741 atcggctatc tttaaaaatg cggcggatca tcaaacaaaa tattattttt tcgattcttg
    19801 ttattctctt gttgattgct tctaacttcc tgcaattttt aaatctacct cttggagttg
    19861 taggacacga aggaagcaca atattggtta ttctgaatgg cttgcgttta ctaagacctt
    19921 tacctgatct ttctgattct tcaaaaaaat gtaagtcttg tccactatac aaagcgcggc
    19981 tgtcttctgt caaataagca aagtgttttt ttatgatttt gtttatttta tgaaaaaaac
    20041 agcttatgaa taggtcttga aggatgaatt cctacttttg taactttcta aaaacatgtt
    20101 atgattgttt cgaagaaagt gaggaatata tttcatggaa agttggcttt ttttaggaat
    20161 tattttactt gtagcaattt tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt
    20221 tttgattatg aagctcatac cagcctcccg ttcctttttc ccttggcttc aaggcaaagg
    20281 aatcaatatt ggggtgacga tcatcacagc ggctattcta atccctatcg caacaggaga
    20341 aatcggtcta cgtgatctat tagaagcctt taaatcccct attggctggg tagctgtttt
    20401 ttgcggtggg ctagtggctg tcttatcttc caagggtgta ggattgattg cttctgatcc
    20461 acaaatcact gttgcacttg ttttcggcac gattttaggt gttgtcttct taagaggaat
    20521 cgccgccgga ccggtcattg cttctggtat cacttactgt atcttacaag tatttgcttt
    20581 aggaaaatag aacaaatttt gtctatttta ggagattctt aacaccccca atttactgat
    20641 ccgtgatata ctgacaacga ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa
    20701 caatcggctg gtttcttcta gcggtacttt tgattctggg cttagcttat ctattatttc
    20761 ctgatttgat acaaaatgtg tttgatactg ctttttgatt gacctattaa aacgatatag
    20821 gggatgctcc tgcgatgcag gggctttttt taatcaatgt tttgtaataa tttaggccgt
    20881 gccaatgaga cttcctatag aattcgatgc tcttttttct ttttggctct tctgctcttt
    20941 tttgttatac tagattacag tggacaaaag aaagaatgat cgcatccact tcaacgaata
    21001 tcaaaatgga agaattagtg cctgttttca ggttactttg aaaagaaagg aagacgcttg
    21061 tggaaacttt tgaaggtctt ttaattgata cgtacgtttt acaagaacag ccaatgatgc
    21121 tgactgggat caacttgcag cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct
    21181 atttggcaaa atatatctta cgtttgcccc taaataaata tgtgctggaa gtaagtggca
    21241 aatggaaccg tcggaaccag ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat
    21301 acattaaaat aatcggtaca ccagaatagc ctttactgat caaaggatac tggttataaa
    21361 ataactgcag tttcttaata gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa
    21421 aaaaactttg caaacggttc tctttgttat ataataactt agtaccaaac attcccattc
    21481 aaactttgtt taaatagata aataaaaatc tagtttggtt tgacgctatt gcctcccaca
    21541 atagcgtctt tttgattttt ttcagcttgt actatccgtt tttactgaaa aaaaatccta
    21601 ataaaagact gttaaggaaa aaaacttgtc atgttcatat tgcaaagcac gctgccttta
    21661 ggtataataa tgaatgttga attatgcggt aacacgcgta agaacttaag ttatcggagg
    21721 tgaaactaat gccaaacatt gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta
    21781 atgtgaaaaa ttcatctcaa acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg
    21841 ctgtagcttc aggtgctgac aacgtagatg cgttatataa agaagctgtt aaagctattg
    21901 atatggctga atcaaaaggt ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt
    21961 taagcaaaaa aatcgctaaa taaaagaatc ggcttcggcc gattttttta tttgtcaaaa
    22021 aaaggacaaa aacaaacgac cttcccgcat gctttgccct cgtctttctt cttatcgatt
    22081 tcttaacaac tgccctgtgc gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg
    22141 tcccgttttg atttgataat ccatctcaac taattgatca aacaactgta ccagcaaatg
    22201 ttcatcgaat ttttttactt cctgcatggc aagttttaca cgatatggat ggattttcaa
    22261 tgtttcagca atatttgcct gctgataacc aatctttaca agaaattttg tctgtaaaaa
    22321 taaacgtaat tgagaaagca aaatcgcatt aattttgatt gtttcttccc cttgcataac
    22381 taaatcttcg tataaacgca aagcttgttc cgtcttccct tttaaaatat attgggtcat
    22441 gtcaaaaata ttatgttcta acgttttagg aactaattct tctacttctc ttaccgtgat
    22501 ttgctgttga ttttgtccat aaaggaccaa tttctgtaac tctctcatca ttttggataa
    22561 atctaagtcg gtcaacctca agaataaatc aattgcactt cgttccattt tgattgagga
    22621 attctctaag gtattcatca gatattgacg tacttctttt tcattcaact gttggacatt
    22681 gatgataatt gctgtttttt tcaattgttt cgttactttt ttccgttcat ccaacttttc
    22741 atatggcgca aagaatacta atacagtcga ttctaatggt tctttcaaat aagccgtcaa
    22801 taaatcagta ttttgttcga ttccattgtt tactttttca cctgttaaaa aataaggatt
    22861 ctctacgaat actaaccgtt gatctccgaa aaacggcaac gtctctgctt cagctactac
    22921 ctcatctaaa cttgcatttt ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc
    22981 taattggatt tttttgacaa tttctgtccg aacttgatct tggagaaact tttctgttcc
    23041 taaaatcaag taacaagatg caagtttttc tttacgtatt ttttgtaact ctgcttgtat
    23101 actcaaatta ctcaccactt tcaaaaatta tcctaccatg agcagatgag aattactagt
    23161 ctatcattgt ttccactagc ccatgaggat gaaaaactgg atgccagcga taggtgatca
    23221 ttccttgttg atccgttcgc catgttttta tctgattttc ttctagtgtc tccaatactt
    23281 cgctgtgcgg gtgaccataa cgattattta cgccgcagga tataatccca tgttttgctt
    23341 caatatggga aagaaaatct gtgttacttg acgtacggct accgtgatga cctaatttga
    23401 ctacatcggc tttcagatta ggataaatca aaagcaattg tttttccatt gcttgattca
    23461 aatctcctaa aaataaaaag cgcctattgt tatattccat ccatagacca atcgaatctt
    23521 catttcttcc ctctcccttt tcaaaaggag tcaatacata aagatcataa taaccgctga
    23581 tcctgtcccc atgtttgatt tcaatgatct cagtaccatt tttttgagct tcagataaat
    23641 gcctttgtaa tggctcattt cgtccagcgc cccaaccgat ataaattttt ttgatagtaa
    23701 aatgctgaga taacgtgggt aattctccca catgatccgt atcgtcgtgt gtcaagatga
    23761 gtttatcgat tacacttatc ccctgacctt ttagatatgg aacgatattt ctttcagcag
    23821 gaggacgtat cataccttgc gcccactttt ctctttcaaa gtgtaactga cctccagtat
    23881 cgatcaaaat cgtttctttt cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga
    23941 catcgataaa agcgatcgtt gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag
    24001 aaaaaagacc aacataaaac aagtgtttca caggttgttc tttcaaccta tccatacaaa
    24061 tcagtaatcc gaaaatatac atcccagata gcacaagggg caaacgtcct ataatgagag
    24121 acgaccctga agaaaaatcg attattcgct caaataagat taatccctct tctatcagca
    24181 aaagtaaagt ttccggcagc cagcgattga agagtgtgag aaataacaca ccaggaagaa
    24241 aaagaaactt gaataccggt gcaatcagta atgtaaaaag actacctaaa ataggccact
    24301 cataaaaatg ccatacaatc aacggcatcg tgatcatagt aaacaggacg tgagaggccg
    24361 tttttttcaa ccaatctcca tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac
    24421 taagctgtcc acccgcatga aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga
    24481 caactgcaaa taagtccatg gtggaataat gcttattcag caatttgact agcaatcgca
    24541 ataaaaacag caaactagca cgcaaaacag aaatagaact tcctgcaata gaaacataac
    24601 agatagttag tagactcaac ggaataatac tgacgtgtag tggccatccg atacgtcgaa
    24661 ataaataata cagccaccct aaataaaatt ggatgtgtag acccgataga ctgaacagat
    24721 gcagcaaacc actctcccta aaaatttctt ctggttctct gaatcctgca tctttatagc
    24781 cccataataa agaattaata tatacacttg ttttttgcgg aaattcagat tggacaaagg
    24841 agataaaaga tcctcttttt ctctgaagcc agtttcgcca gtgattttgc atggtgatct
    24901 gttcaatctt ttgaatttgg aaccgtccag aaattctatg gttcctgaag taagcttctt
    24961 ggtcaaaatt ataaagattc cgcttttttg ctggagagat gaactcgcct tcagcagtca
    25021 attctattcc ctttcctcgg aagttaagcc atgttttcaa ttctgcttct gtgtttactt
    25081 gataagaaag ctggagcttt tgtttattct ctgataacct tccttccatt gtcaaaaaat
    25141 taccatttgc acgaatcgta tctgtaaata cagtaatttg cgcagatgtt gttttttcct
    25201 ctaaaaatga atactcttgt agcctataag aaatggatac cgttatgcta cacaaaatac
    25261 acaagtacgc gataaaacgt ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa
    25321 gcaaacgata accccaattg tcagtgtaat acaacaaaca gataatcatc gacgtaattg
    25381 ccggaaaaat caaccgattt cgaacatgcc tttctaaaac gctagacatg ttttcctgtt
    25441 aattcatttt gaagttttga aaaatgctgt gagtctaaag caacttggtg tacagataca
    25501 cctaattgtt cgatcaacgc ttgggcataa ggatcatttc gatagtcatg gagataattg
    25561 atccctttca caccagcttg caacaaagcc tttgtacagg ccaaacatgg aaaatgtgtg
    25621 acatagattt ctgaaccttc cgtcgggata ccaagttttg cacactgtag gagtgcattc
    25681 atctctgcat ggatcgttcg caagcagtgt ccatccacga cgtaacaccc ttcatctatg
    25741 cagtggacat ctccactgac tgctccatta tagccgccag caatgatcct tttttctcga
    25801 acgagtgtcg ctcctacttc taaacgtgta cacgtactac gtaaagataa cagtactgcc
    25861 tgagccataa agtactggtc ccaaggaatg cgttcattat gtgacttatc agtaactttt
    25921 tctttattcg tatcgcatgc ttttgaagat ttttctaaca tgttttatat tcctccgttt
    25981 tttcaagttg attaaaaagt actcattgct taaacaaaat attctttaaa tataacgaat
    26041 catcctgtct gacaaaagat cctacccttc actgccgaaa tgaagtccgt catccattag
    26101 cccattataa aaattcttcg aatccatttg ttcaagaaat tccgtataaa aatcgatact
    26161 tcacaatcat ggcgttttgc tacttctgtg agaacagaga tatattcttt gatcaacttg
    26221 ttattccgat atttttgttt tgtctcatcg actgctggtg gcgtgataaa gaaagcatga
    26281 tcagaactat agtctttttt cagctgaact atcatccact gcagattttt tggtacgttt
    26341 ctttagaaag ttgtttatgc tctgctagat cattcgtccc aatgagaata aatactttat
    26401 ctgctgtata gctaagtaca tctttggcaa agcgttctcg tagagcttga ctgttattgc
    26461 cgctgacagc cgtattcata atatgaatcc cagatatttt attttggaga tagacattca
    26521 gcattggttt ttttaatgtt tctttgcgaa caatcaagct atttcctatc caaaaatact
    26581 acctcctcgt cctgccaagc tcttagaatt ggattattct tagtatccat tagcgtccat
    26641 ttattgctcg tgccagtcga ttttgaatca ttctatttaa ttgtaacgaa gttttttagt
    26701 ttttcaactg tcttttcacc gataccagaa atctcctgca ggtcttccac tttctggaag
    26761 gatccgtttt cttctctaaa tcgaatgatt tcttgcgctt tgactgatcc gatcccagga
    26821 atctgctgaa gttctgatgg agtggctgta ttgatattta ttttgggagg ctcggaatct
    26881 gctgtaggct gttttccttc cgaggatgcc aactgatcaa gatgttctgg cagctcttca
    26941 cctacagcag ggatatatag gacttgctga tcttctatct tcgcagaaaa gttgattcga
    27001 tcgctatccg cttcttctaa taatccacct gcacgcttta gtacatcata gacccgttct
    27061 tctgaagaaa aggaatagat accaggctcc ttcacacttc ctttgatatc ggtgtacatg
    27121 acctttggac gagcatcctc ttctacatcg tttccttcag ctgatggatc ttctccactg
    27181 gaactaaacg aagaaagtat ttcttttgca gcagtctctt ctgtttctct ataaaatagc
    27241 aaatttgata ataaaaaacc acttgcgata caaaagataa gaactatcat agacatccaa
    27301 atgatcaatt tgggtgaagc tagtattttc ttgctgattt tttctatcat tgttcttttt
    27361 tcaaagaatg accagtaaaa atcattcatt actcctttca tttaaaattg aagaaacagt
    27421 gagctctggc aaaatcgtcc cttcaacatc taattacgtc aattttttct tttgttttta
    27481 tttttatcgt gaa
//
LOCUS       DASHFF010000033        26612 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.033, whole genome shotgun sequence.
ACCESSION   DASHFF010000033 DASHFF010000000
VERSION     DASHFF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.033"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..504)
                     /locus_tag="U2I75_001581"
     CDS             complement(<1..504)
                     /locus_tag="U2I75_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696720.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQ"
     gene            complement(728..1612)
                     /locus_tag="U2I75_001582"
     CDS             complement(728..1612)
                     /locus_tag="U2I75_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1696721.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1711..2148)
                     /locus_tag="U2I75_001583"
     CDS             complement(1711..2148)
                     /locus_tag="U2I75_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696722.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(2365..3276)
                     /locus_tag="U2I75_001584"
     CDS             complement(2365..3276)
                     /locus_tag="U2I75_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1696723.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(3733..4161)
                     /locus_tag="U2I75_001585"
     CDS             complement(3733..4161)
                     /locus_tag="U2I75_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696724.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(4189..4827)
                     /locus_tag="U2I75_001586"
     CDS             complement(4189..4827)
                     /locus_tag="U2I75_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1696725.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(4831..6813)
                     /locus_tag="U2I75_001587"
     CDS             complement(4831..6813)
                     /locus_tag="U2I75_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN1696726.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6993..7658
                     /locus_tag="U2I75_001588"
     CDS             6993..7658
                     /locus_tag="U2I75_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1696727.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(9593..12742)
                     /locus_tag="U2I75_001589"
     CDS             complement(9593..12742)
                     /locus_tag="U2I75_001589"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HEN1696728.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(12755..13954)
                     /locus_tag="U2I75_001590"
     CDS             complement(12755..13954)
                     /locus_tag="U2I75_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN1696729.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(13944..15539)
                     /locus_tag="U2I75_001591"
     CDS             complement(13944..15539)
                     /locus_tag="U2I75_001591"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEN1696730.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            15664..16449
                     /locus_tag="U2I75_001592"
                     /pseudo
     CDS             15664..16449
                     /locus_tag="U2I75_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
     gene            17158..17517
                     /locus_tag="U2I75_001593"
                     /pseudo
     CDS             17158..17517
                     /locus_tag="U2I75_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(17812..18945)
                     /locus_tag="U2I75_001594"
     CDS             complement(17812..18945)
                     /locus_tag="U2I75_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1696731.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(18936..19604)
                     /locus_tag="U2I75_001595"
     CDS             complement(18936..19604)
                     /locus_tag="U2I75_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1696732.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(20060..21046)
                     /gene="lacD"
                     /locus_tag="U2I75_001596"
     CDS             complement(20060..21046)
                     /gene="lacD"
                     /locus_tag="U2I75_001596"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN1696733.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(21059..21985)
                     /gene="lacC"
                     /locus_tag="U2I75_001597"
     CDS             complement(21059..21985)
                     /gene="lacC"
                     /locus_tag="U2I75_001597"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEN1696734.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(21998..22513)
                     /gene="lacB"
                     /locus_tag="U2I75_001598"
     CDS             complement(21998..22513)
                     /gene="lacB"
                     /locus_tag="U2I75_001598"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEN1696735.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(22532..22960)
                     /gene="lacA"
                     /locus_tag="U2I75_001599"
     CDS             complement(22532..22960)
                     /gene="lacA"
                     /locus_tag="U2I75_001599"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEN1696736.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(22980..23753)
                     /locus_tag="U2I75_001600"
     CDS             complement(22980..23753)
                     /locus_tag="U2I75_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1696737.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            23918..24397
                     /locus_tag="U2I75_001601"
     CDS             23918..24397
                     /locus_tag="U2I75_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1696738.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            24454..24756
                     /locus_tag="U2I75_001602"
     CDS             24454..24756
                     /locus_tag="U2I75_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEN1696739.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            24778..26235
                     /locus_tag="U2I75_001603"
     CDS             24778..26235
                     /locus_tag="U2I75_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN1696740.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
BASE COUNT         7946 a         5370 c         4371 g         8925 t
ORIGIN      
        1 ctggaactcg atcgactgag gtatctcatt gatataaata tggtgtgtgc cgaaattgtg
       61 agcgaatcca ccaaaatcaa taacgatatc tcgtttgatc tctaattccc tgtctaaagt
      121 caaaggctga atcagtcgga tcttagttac tgattcgtct gcgagaagct gcgccagttc
      181 ttctgttgtg gcagcttcgc tgtacccatc cgctgaaaca gctcttgcca gtgccgcttc
      241 actgctatca gacgtttgtt cagctacttc tgaggtctca cttgtcttat tttcttcttg
      301 ccttttatta gtagaagcct cactttgaga ttctgtagtt tcagaagtaa tcgattcttc
      361 tgaaaaagta gtcttgtcaa aactttgatt ctctgattgc gttgattcgt ttgttttctc
      421 tacatcggca aaaacggatg tcaccatcaa gagagggaag acaaacaaaa tcgacgcaat
      481 cagctttttt acacattgat tcattctcca ccatcctctc tgacgttttg acaattttat
      541 tttatcgttt taatctattt ccaattgtac tttcatattt aaaatatgta tttttatgga
      601 aaaatttttc tattaggtgt aaatttcttt tttgtacaaa aaaaaggctg tgacaaaagt
      661 cttgtcacag tcccttatcc cgaacaagcg gtgttctccc gccaactcct cggtcacaac
      721 ctccatttta acaataaacc tgtaaatgtg agggcaatac tttgattgtc acaggtaatt
      781 tatccccttc atctccatca acattcgtcg tcaattcttc ctcatcagct aatgacacaa
      841 ctaattgctc acaagtaagg taggctagat ttttcgtaga ttcatctact ccttttaata
      901 ggtctggcaa tgttttaagc gtatcgatca aagaagaatc ttttaaataa accaaatgga
      961 gcttaccatc atccactttt gcatttggaa gtaatgtttc aaaaccgccg actgagttag
     1021 ttagtcctat cagcaaggta ctgctctcaa tttcttcctt ctttccatca acttctacgt
     1081 gaaacgagta ggattgtgtg gaagctaact gtttgattcc tgacatgaaa taagctagtt
     1141 ttccaaattt cgtcttcttc tccgcatcta catcattgat tgattcaggg atagagccaa
     1201 ttgcgacgac attcataaaa tagtcgtcat ttatttttcc aatatccaat ggcttgactg
     1261 attcaatcga aaaatgattg atcgcttcct caggttctag cggtatccct aatgctcgag
     1321 ccaagtcatt caccgtaccc aatgggaaaa atccgaaatt tggccgatgt tcttgttcag
     1381 cgattccact gattccttcg ttgactgttc catctccgcc catgacaaac acactatgat
     1441 acccttccgt agccgcttcg cgagtaaagt tttttgcatc ccctgctttt tttgtatgca
     1501 agacgaccac ttcatcaaac accgattcca attttgctat cgctaattgc tcaaattcct
     1561 ttgcttgttc accgcctgaa ctggggttta ccactaataa aacttttttc atcactttct
     1621 ttcccttctg actgattgat ttctttaata taacgatagc ataaagaaaa aaattaggct
     1681 gtgatgaagt actgttcatg aaacattcat ttattttttg acaagataaa acatccatac
     1741 tatgaaactg aggaaaatta aaacaatgat ataggaaata aaagaaaatg ataggcattg
     1801 tatacctaat aaagtaaaaa tcttaggtgg cagataaaaa cacaaaaata gcaagagaat
     1861 gtaatagcgt ttcttccact ttttgttatc agaccttata gcgatcagag gaataccaaa
     1921 tgcataactg aaaaactctt gatgtactgt taatatgtca cggccttgaa taataaatgc
     1981 gacaatcccc gctataatta gtgtgcctaa caccaaaacg gaaaattgat gaatatattt
     2041 tatatacaaa tgatccattg tacttcctgc ctttttattt gtgagaaaaa caaatccgta
     2101 tacgtgaata acttctagaa ataaaagaaa tttgcaaaac cactccattt atctacttct
     2161 acgtgcattt tttgatattg ttttctttat ttaagattat acagaaaaat atctagggtc
     2221 gctggattct aagtaaaaaa aatttttaaa ataaaaagaa ctaaatcaag tagtaattaa
     2281 actactgttg atttagttct aataaaaaga tttagatgaa cagttttatt gatttaaata
     2341 gttcatattt tttatttata ccgcttattt actacttgct gtactagaat ctgaagtgaa
     2401 tgtactcaaa acattttcaa agtctttgtc tttgattttt acattagcgt ctttcaatac
     2461 ttcgccaatt actttattct ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc
     2521 tttcaattct tttttgtatt tatccatgtc gttgcctttg ttttggtttt tcaccatttt
     2581 aaccacataa tattccgtag tataagttga agcattggta gaagtgatca catcagaaac
     2641 ttgaccatct ttcaacttaa aggctgcttc tttgacttct gccggaaccg ttgtagtagt
     2701 ggaatcaaat ttcacttttc cgccatcttc ttttgtcgta gtatcagttg acttgtcttt
     2761 ggctaattta gagaaatcgt ctcccttatc agcagctttc ttcacatctt ttgcatcatc
     2821 ttcacttgct acttggataa tttgagcttc cacttctgga tggaaagatt cccaagccgt
     2881 ttttaaatct tcatcggtca atttgatatg tttctttaga ccttcttgaa aggctagact
     2941 ttgtttgata ctgtctttga atgtttgttc tgtgtaacct gcagatttca attgtgaatc
     3001 aaatgaatca cccaattgtt tctttgtttg atcatattga gaatcgattt ctttggttga
     3061 tacgtcatct ccatatttat cttcaaatac ttgataaacg atcaactgtt gcacagcgga
     3121 atctgctcct tgcatttttt tcaagctatc cattacttct tgttcgctga ttgtaccgcc
     3181 tttataacta acggctgttt tttcaccggc attcgtacag cctgacaata agccagtgcc
     3241 tagtacgatg gcaagtgcta aaaatctttt tttcatttat caattctcct acttttcgtt
     3301 ttttgcgaat tgctaaaaac aaaggtgttc aagatgaaaa tcatcaagaa cacctttact
     3361 aatctgctcg atacgtcctg aatagatata aggctacaca atccagaaag ttcgctcagc
     3421 agaataaatg tcaagtagca catcacacct taatatacta cttcttttgt taccgttttt
     3481 caagtctatc ttgcgttccg tatttaacgt aactaattct tagaacaaat gttatcgatt
     3541 ttgtttttct tcttccgctg ctgggatatt caaatcagac acttttatgt tcgattttcc
     3601 agacattttt ttgttgtttt tattatgata tatttttata caactaaaaa tgaatatatt
     3661 aaaactacca aaaataactt ttaacgctgt ttcattatat gttttttcta ttttatataa
     3721 acttttggat acttaatacc aaccggttgg attgtaccaa catgagaatg tgtcagttct
     3781 atttcctgtc gcacttgcat aggcccatac tccttttgtt gcatttgctg atcgaataga
     3841 gtttccaact ttcgcttttg caccgtgacg attatagaca cgactatagt ggttagaatg
     3901 acctgttttt ctcgtacaag ttgtatcact ataataaacc catcttccag agctatgatt
     3961 aattgctgtt ctagaaattt cccggtctac tggtaaattt gacttaataa tattaccttt
     4021 agcatcaaaa tgaatctctc ctggctttaa agatttgtaa tagatttcac cgtgaactga
     4081 atttgcttcc acttcagatg acatagcgtt tcctccaaca aaacctatcc ctagtgcaca
     4141 aataataact gcttttttca taaaacgaca tcctttcctt tttgattttt aaagcatgat
     4201 cttcttgttt cctattttgg tcagatatgg atcatgcgtg actacgataa ctaacgtacc
     4261 ttgggaagct tgttgcgata agatttcaac tactttatcc ttatttcttt tatccaatga
     4321 cccagtcggt tcatcggcta aaatgatttt gctattcttc aataagactc tcgcacaggc
     4381 gattctttgc ttctcaccac cagataatgt gtagataggc gtacttagct tcttgtttat
     4441 atttactttt ttcaatgcct catactttgc tgttcgctta tctgaaacat ttaaattcga
     4501 aaacttcaat ccaatatcta aattaaaatc gattgtttca tattcaatca acccaaaatc
     4561 ttgaaaaaga aaagatatgg tatttctttt tatcaagcgc ttctctctcc cagttaattg
     4621 tttcgtctct tgatcgaaca aatagaattg tcctgaactg aactgatcat tcaatcctaa
     4681 tatattcaat aacgtacttt tacctgctcc actttctcca acaacagtca caagatcccc
     4741 agcatccgca gtgaaacgat ccaaatgaag aatcgttttg tttcgaatga tttttttcgc
     4801 accttttatc actatcaacg gctccaattg ttaacctcca ttcatcagca ataactgact
     4861 tttcttttct gtagacaaaa taaaaacaac tgaaagaata aaatcaatga agctaaagaa
     4921 aaaaatatta aaaagatatt taggctctct ttctataaga aaaaggaaga ttacacttgt
     4981 cgttccccat ttgatgaggt tcatcatgaa gaaatcttta tacgtttcaa agagcgaata
     5041 accacttgaa cgaaagatag cgattttccg acaattgtag acaaaatata tgcataacat
     5101 gacatagctc aacaagaaga aagaaaagaa agtgagtaaa aatatctgta tcaagtaatg
     5161 aaatgatctg cttaacactt tgacagctat ctcttcagtc tgagtatacg gaaatgataa
     5221 ctgcaagtta tcacgtaact gactttcttc aatcagctca aataaccttt ctttcgacaa
     5281 agagcctatc ttgatcttta acggtggata gatttctccg ctgagaatat ttctttccaa
     5341 atacatactg tttttcaatg tcagtatttc aacaaccgga taatgttcga tccaggggat
     5401 agaaggaata aatgtataaa tgggttgatt gttttttata aatagaaatt tcggttcttt
     5461 ttcactgatt tcttgataat attctgttat ttgcgccagg tcatttttta aatctgtatg
     5521 tctatatcgt tcagggatca atataacagg gtgcgtttct ttttcggtta tttggatcaa
     5581 ccttccagag acatcaatca aaggaaattt cttaagataa ttcagattta tttttatccc
     5641 tctgccaaac tgttcatttt cttcattcaa gtaatctgat acattgacta atatacttcc
     5701 gtttttattc aaagtttcat ataattcctg atctgctgct gatgcaaaac gattggaata
     5761 aataaaatcc acgtggtttt tcccgatggt tactggataa aatacatgat agtcctcacc
     5821 agcgatttct tttggtggtt catgggaaac agctaactgt gaagaagaaa aaacgatagt
     5881 tgctaattcg acattggcaa cggtcaaaag aagagaaaac aacaatccaa atgtaaaaag
     5941 aatgtattgg atatagttga acgcttctct taacgaagcg attttttcaa ctaataaaag
     6001 agaaatcaaa gagataccat atattccgct gaacaagaaa acgtacatct ggaacaaata
     6061 aagaaattgc tccttataat acataaaaat aaaaccacca atgattcctg caattatgaa
     6121 tgtaataatg attttctttt tccccaataa acaccacaat acttgatgta atgaaaagtc
     6181 attattcaac aataaactga tttctttatt catcaataag aaaaaggatg ataacaaaag
     6241 gaataaaaga atcaatccaa tagttaaata cactcgataa ttatatacgt ctgtatttga
     6301 aataaccgaa tacgaatagc tattttcaaa gtcttcaaaa atattagtgg cataatcagt
     6361 gtgaaatgct tgattgaatc gatcagtgaa tagtgaaatg acccgttcat aggcttcttt
     6421 ttctctcgtc atcaagaaaa attcaccttc aaaatctttt attttgatcg attcttcaat
     6481 ggacgttacg cgaaaggagg tattagaata gggtaccttc tcgtgagaag atttgacatc
     6541 cccttgataa tacaatgtca tttcaagctt tggctgtatc ttgaagttcc aagatgtatc
     6601 tacttcattg gattcattta ctgttttttt catataagaa agctcagttt ctgatgagac
     6661 attttcaaat accatgatta gctgctcata ttcttcttgc ccaataaaat gttgtggcaa
     6721 tcgaatcgga gtataagtat ctgtttgatg gaaaaattca tttgtccgat agagtacctg
     6781 tttatttata ctaaccaagg caaaaccact catgataccg gtcactatga ttgtgagtaa
     6841 aactgtgaac ttttttttga ttatcaccac tctcccttac ttaaatgatt ctttgataaa
     6901 atcataccgt ttttcatcaa aaaaacgtat ctatcaagtg tataggtcat gtagaataaa
     6961 tctatatact gtagaatcga aaggagaaga agatgaacac cggagaaaaa atacgaactt
     7021 accgcagaaa ttgtaagctt acgcaaaaag aattaggcga aaaaattggt gtttccgata
     7081 aaacgatttc tagttgggaa aattctcgta caatgccaga ctttgaaatg ctgtcacttt
     7141 tacatcatac tttaggtctg ccgattgatt ttccttctat tgaatcagct accgaatcaa
     7201 tagaaccaac attgccagat gaacctcttg ttaacaagaa atcttattca tcaaaacaac
     7261 ccttctcact caaaaagaca ctatctttct ttctgctcgt cttttgtttg gtctatcttt
     7321 ttcttatcaa ttatcaatac aaaaatgaaa cgatcgatcg attcaatcct tttttgaaac
     7381 cacaaatcgg ctataccaca ctccctgtag gatacgtgga caattccatc gaacgttggg
     7441 tatgtaatga tatctttggc catggtacat ggctgtcttt tcacgctgga gaggtagaaa
     7501 aagagaaaaa ccatgctgta atcaaacaca aaggaaaaca cgtcatgaac gttcgcctgc
     7561 tcacttggga agccattccc tcagattacc aagaaatcct cagccaagac tatgacgacg
     7621 ccttaaaaag aagccctcag gagccagata ggcattaagt aggagatgaa cgtagagata
     7681 gaaaaaatca atgcacatga tcttatccaa aaaacagggg tacaaattaa ttgaaaaagc
     7741 cttgagatga aagttcctaa ataaaaaagt tcagaatgat aggtatttct ggcttatagt
     7801 ataatattta cgccacagcc tcccaatgat tctttttgga atcataatca aggatggaca
     7861 ttcacaaaaa tattttgctc attcgatccg aatagattaa cgaccaatct agtaagtaat
     7921 atagattggt acgtagtcaa gctgcacata atttagatca attttagttc ttttttatat
     7981 ttctatgatc tgctcattta cttttatatg atggcttaca gtaacataaa taaatatgat
     8041 aatcgctggt agtttatcca agagtactca ataaaataca aaatggattg ttcaagcgcc
     8101 tatcataatg ataaaatgaa acaaataaac gatacaagga ggaagagact ttgagtaatg
     8161 gcagaacaat tttttcattg taaaaattgt tcagttgtta atctatggga tttcggtagc
     8221 atgggcattt ttttatagag gaatcacacc aacctggccg ttttatctct tgttagtggg
     8281 aatattagta ggaaattttg cccctcttca ctatcgtacg aacttttttc caactgattc
     8341 tggccttttt cttaaaacaa agacgcgaaa attagaaaac attattgaaa tgacaattac
     8401 ctgtataatc attttcttag ttatattcct aaacgtcttc taataagaat ctcttaaaat
     8461 tagcttgtta cagttagtta cctagtttat ataacacagt tatcttatct tccttatcaa
     8521 catcttttgc caactctttg tcttaaattc gaaacatata agcagagcta cgaacaccaa
     8581 gactgtttcg gctctgtttt ttgcatcaaa ttcactctta tatagctgat aatctcggct
     8641 ctttaaaaga aattattttc gcttcatttt attataaaaa ggctaggaac cttgatgtta
     8701 tcaggttcct agcctttatt taagtattac cccacagacc cttccatata gatttttaaa
     8761 ttacacacta gtttaaacta aaacaaacaa ggaaaaactt accatttaat caaaaaatta
     8821 gctcatatga atatattaga atgtaaatta gactggtcaa aatctggtca aaaaagagga
     8881 cgaatacagg tagtcctctt ttttgttatt ttgatattct aaatcatttt ttattggatt
     8941 attcccttgt tcgatataga cccaaattga tctatcaata attatcaaac tgagtcatat
     9001 agagacaaaa atttcaaaaa tgacctgtta aatggtcatc gaatatcatt taaaatcaat
     9061 acgacggagt cagacacgga gtcaaaacaa gcccgctgag agtaaaaaat atacagtccc
     9121 ataggtaaag tgccacagga ctgtatattt ttttatttga gattacctga aatgattcca
     9181 tttaattttt atctcatttt ttcctactag ttagtatcaa atactctcct attttctcaa
     9241 taaactattt tgggttacat ggtatctgaa acagctaaaa ccttgtcatt atagcaaaaa
     9301 atcactttat ctccgtttca tttcagatac atttcactta agcggattcc aaaaaaacaa
     9361 aagaaaaagg aacccctagt tagcctatgc tttttattgg ttcctctcaa aacacggtca
     9421 tataacaacg gggtggggta aatgaatgaa aaaggtggag gaaacattat tcaatagctg
     9481 tcttaaatac ttcgttataa aatgtgaaca aataaaagac atctatccct attttctaca
     9541 cttccacttg actaccatct ttgaatcgta caattttctt aactcttctc gtttacctca
     9601 attcattctc aagtggaatc aactcatcat ctaacatttt acgccatgct cgcttcattt
     9661 cttgtgggta tttaaatggc ttcgcttctg gatgtttggc tttatatcct tcgtaatcct
     9721 tactgttgat aattgctttc atattaggaa ttggatcttc acctggtaga tattgcagat
     9781 tagaagcatg taattctttc tcagaaacaa accactcatt ggaaaagaca tgaatcaaat
     9841 catcactcgc ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca ttactatcta
     9901 aagtccccac atcaaatgca tttaaaattt gtcggtaaac ttctttgact ccttctgttt
     9961 tattcaattt agccaaagct ttttcaattt cttctcgtgc atctgaacta ttcgcagcat
    10021 aagttcctag taattgatca atataagttg aatcaatatc atacaactta gtggtatttt
    10081 cactataaaa ttcgatcgtt gaaaaatctt ctccttcttt ttctggttct tcctctatta
    10141 atgaaccctt cacggtctcg tacacgccaa tttgttcttc caaaagttga atttgattgt
    10201 tcaaagttgg agatttcacc atgtcatcat tgtaatcgtc ataagtcact aacgattcat
    10261 aagcacgatt caattcttga aatgctttga cgtactcgat acggatttca agtggcgtat
    10321 gttcatcaat ggattccatg gatgaaacct cttttaactt accatatgct tgtttgaatc
    10381 tttttttgga ttcctgataa cttggataaa tcaaacctga ttcttctttt gcttttgaat
    10441 acagttttgt cgcatcctcg acattcttct ccattgtcgc tggcttacga aacgtcacaa
    10501 tcaatccttt tgtcttatca ggatacgtac gattcgtacg tgaaaaagct tggatcaatc
    10561 ctgcatactc tagattacga tccacaaata gggtttgaat cgtaggggcg tcaaaacctg
    10621 ttaatagacg atccactaca atcgctaaat cgatttgttt cccaaattgt ttgaattccg
    10681 ctctttttcg tgccaaacga ttattgatgt ctccgttata acgctcaatg tctgctaatg
    10741 accaggaagt atcataatac tgattatatt cctcaataat tttttgcatt tcttcttgtt
    10801 gggcacttga atctcggcta ttttcttcta atgaataggt aatagcgatt cgtggaaaat
    10861 caggatcctc catcgttcgt ccttcacgaa tgagctgatt gggaaactct tttgttaacc
    10921 aatcaggctc tttcgtcatc tctttgatgg cccgataata ctttttggcc atgtcaatgg
    10981 aactcgtggt taagattgca gatttcgttg gcctgccatt tcgaaaatca aattttgtat
    11041 aggcattatc tggacggaac attttatgaa tgactttttg gatatgttca tctgcttcaa
    11101 aaacagctgg gtccaaataa ctttcttttt tgacaggttc catttgatca atcatacgat
    11161 tgatctgttc tgatgaatac tctgcatatg tttctacttc ttttaatttc cgatagattt
    11221 tattctctag tgaagtcggc tccacggtgt tttcatgctc tacttgaaat cctaagacag
    11281 agccatcttc tagcgcattc ttaatggtat aggtatgcaa gacctctcca tactgatcat
    11341 gggttgtacg tgccaattgc cctttggctt gtttccgatt ctcaggaaaa atcggtgtac
    11401 ctgtaaagcc aaaccaagtg gatttcggaa acattttttt gatttcttcc atattttcag
    11461 cacttaacgc tcgatgacac tcatctacta caaacacgat atgttggccc attaattttt
    11521 ggaagcgatt ggttcctttt ttctcttctt gtttctttgc atatttcaac gccgcatcta
    11581 gtttttgacg ggttgtaatg attacaacat ttgcattggc atctgccaac agggtttcac
    11641 ttaattcttt ggcacttccc gttccaacga tcaagctatt cgctttggcg ttcccggaag
    11701 aaatcccagt attaaattcc gaagcaaatt ttgtaaactc tgtggtggtt tgattatcta
    11761 aatctttgcg gtccactaac atgatcgttc gatcgatccc cgattttcgt gctaaaagct
    11821 tagtagatac aaaactagtc aacgtctttc ctgaaccagt cgcatgccac acataccctg
    11881 attgatgttt atttgcggca ataaacaagg cttgaatcgc atgtacttga taaggatgta
    11941 acaccatgag ggttttatta tcttgatctt cactgacgat tgtataatcc gcaatcaaac
    12001 gatgagcatc cgggatattt aacacttgtt tacaaaactc atataagttt tctacttttt
    12061 cattgtcttt cgttcgccaa ctaaataaaa acttagggtg caagtctttc ggtaaggcat
    12121 ttgcaaaata ccgagtggtt tgttcattgg aaaccacaaa caactgtaag gtcgaaaaga
    12181 tattgttccg aaacatacct tcttctgcat attttttgat ttggttaaat gcctgataaa
    12241 caccatcttt tgcagttact tgtttcagct cgatttggac aatcggtaat ccattgatta
    12301 ataaggtgac gtcaaaacgt cgatcccgtg cttctatgtt acttccacgt tttgcaattt
    12361 gatggaccac ttcataagta gagattcctc caccaatgtc ttgattggaa taaaggataa
    12421 gtgagacaga acccaattgc gaatcttccc gttcaatcgt gatccgagcc atcccatttt
    12481 caccttttaa ccatttcgca gcatcaaatg gggttttcgt tcgcagcagt aattccgttt
    12541 tgatcgtttc aaattcttta tcggtcaaag gaaaatcatt cagttctgct tgattatttg
    12601 agataatttt ttggcgtaaa ttgacccaaa ggtcttcttc tgattttaga tccggacgat
    12661 acgtccactg attatgccct tctccaagta cacgaatcaa ctgttcttcg acttctgctt
    12721 cctcacggtg actaattttc attcttcttc cctcctagac aaacatcttt tgtaagaagc
    12781 tttttttcgt ttcttttagt aaatctaact tacgttgatg aagagcgatc gtatcatcta
    12841 gctgtttgaa gaaagtaccg attttttgtt gttcatcctt tacaggaacc ttaacactga
    12901 tattctcaat atcagaactg ttataagaag gcaatgcccc tacttgggag tatcttggca
    12961 aatttatagt ttcaaacaat attttaccaa aatagatatc ccaagtatta ttaaataaat
    13021 aagccatggt attgttatct attaagaaag ggcttgtgac caaacgtttt ctattaagca
    13081 ttattgctgc gccaacttta gcaaatataa tcgctggcac atcttttatt ggcttccatt
    13141 ttttacgctc aatttgctca ttagatacat aatgatttgc atttctcatt tcatactcat
    13201 taccaatgtt attcatgtcg gaaactttaa aaaatggagt accttcagaa ccaccttgct
    13261 cggcatcagg aaatccaatt cccgattgag ttttacctaa ttctcccaac ttacgctctt
    13321 cccaatcttc cgtaaaacca ggaaaccgga tttctggcac ttttgctccg ttttcaggga
    13381 acattttttg taagaatcct tttttcgttt ccttcagcaa atctaactta cgttgatgaa
    13441 gagcgatagt gtcgtcgagt tttttgaaga atatgccgat tttttgttgt tcttttaaat
    13501 cattgggaat atagaatact gtatctgaaa cttttttcca gtctgaacga ggcatttttg
    13561 ttccagatgt atcatttgca gccttttgat atctatcagc ttgaataaga gaataaataa
    13621 agtccgagtc gctcttctta gctctaaaaa cccagaaatc cccaatcgct acgcctgtaa
    13681 aagttgcctt gagccaattt tttaaatatg gacgtaactt tccatataga atatcatttg
    13741 gtaaaaattc aatccctttt ctattatcaa atttctggct cacatcttta ttcagtctac
    13801 cttctcctgc cacgatatct tcaaattcaa cttttggaag tctactggag tttgtacttt
    13861 tattaagtct aacaactaaa tcaattaact tacgctcttc ccaatcttcc gtaaatcctg
    13921 ggaaacggat ttctggtcgt gtatcattgc tcatgtttga acacctccag cgcaccttgt
    13981 aaccacattt cgttttcagg tgttgtttgc agggaagaga tagcttctaa aagttctttt
    14041 tctagcactt gctgttcttg tcgtatcttc ttgatgtcat ttccgacatg aaccatatca
    14101 attggttctt cttcttcgaa agtatctaca tagcgtggga tatttaagtt atagtcattt
    14161 tctttgattt catcaaaggt tgccaaatga gcgtatttct cgacatcttt tctttcagcg
    14221 taggtttcga ggattttttg aatgttttct tccgatagtt tattttgatt tttcccttta
    14281 acaaattcac gactagcatc aatgaataat acatctcgag tttgacgatt cttcttcaac
    14341 acaatcaccg tcgttggaat cgatgtccca aagaacaagt tcgcaggcat accgatcaca
    14401 gcataaatac tgccatcttc tagtaatttt tgacggatga ccccttctgc agcgccacgg
    14461 aataaaactc cgtgtggtaa gacgatggcc atggtacctg tttccttcaa atgatagaac
    14521 ccatgaagaa gaaacgcaaa atctgctttg gattttggcg ctaatttccc ataacgatta
    14581 aaccgagaat catctaagaa agtcgtatct gctgaccaat tagccgaata aggtgggttc
    14641 attactactg catcaaatgt gtaaggttca tctgttggcc agtccttatt caacgtatcc
    14701 ccattgcgga gattcatttc ttcggcgtcg acaccatgta aaatcaagtt catttttgct
    14761 agattgtatg tcgttgtatt tagttcttgc ccatgatatt taacgttatc tgggtgtgtt
    14821 aagtagttac gtacgtttaa catcaatgaa cctgaaccca tcgttggatc aaaaacacta
    14881 aagaatcgtc gttccttttg atcgagcgtt acgatttgcg ccatcatatc agaaaccata
    14941 tgaggtgtgt aaaattctcc ggctttcttc cctgcttctg aagcaaattg actgatcaga
    15001 aactcatacg catccccgat cacgtcccca tcgtgttcta aaacatctac atcattcaat
    15061 ttcttgatga cttcggtaat cgtcacattt cgttgttgct catctgttcc tagttttttt
    15121 gattggagat ccacatcatc aaataaccca ttaaattgat tgtaggtaga tgccaattgc
    15181 acaaaggctt tgtttagatc gttcaattgg aacgttgctt gttttgcttg atccgctaag
    15241 acgttgaaaa gatactctgg tgcaattgca tatcccaaaa tatcaacgat tgtggcaatc
    15301 agatcttctt ttgattcttc atctgaaagt aattcacgat acaacattgt ttgtttgctt
    15361 actgtgtcat attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaaacgg
    15421 tcggataaat atttatagaa aatcagtccc aataaataat ttttatattc tgacgcatcc
    15481 attttactac ggagattatc tgccgcacta aataattttt gatttaattc tgcacccaaa
    15541 aagatacact cctatctctt atttaaagtt gctatatacc ctcttatttt agcatactcc
    15601 cttttagaaa aagcaaaaaa aaaagaaaaa atcttattct tgataacaag ctacgtgacg
    15661 tatatgccta acaaaaatag aacttttgaa tcaatcttaa atgaaggttt aaccacagaa
    15721 ccggcatcca ttatttttga tgaagttatc gatgacgttg aagatagtaa gtatcataaa
    15781 aatttaaaag aactttttta ttttgcactc aacaatggac caagtttgtt cactggtata
    15841 tcagtacctg atagtgataa tccattaatt atggcaaaaa tttacttaac aaaatggtgt
    15901 aataaataca tcagggatag gaataaccca gcataaaaaa aacctcttaa aacatttggc
    15961 gaaaaagatg ctgctttaac tactagagta gcagcaaatg ctaatattga tgatcaaaaa
    16021 gtattgaatg actacttgcg tggacatttt ttatatatga gcgctgagaa catgaacgga
    16081 agtattttag aggaatattt agccgaagtg ttagaacctg aaggttggat ttggtgcgcc
    16141 ggctctgtat atcgagctgt tgatttctgt tatttaggaa cctcaccaat tcttttacaa
    16201 gtgaaaaata aatacaatac agaaagcagt tcgagttcag ctataagagt aggaacaaca
    16261 attagaaaat ggaatcgttt aaataagtcg acaaaaattt ctggtttgga tagccctatc
    16321 cccaattgga aagcattaat agaaatgact gaagctagta aggaactggc agctaagtta
    16381 actgaaaata gttatttagc ttatattaat gaaaaatcca cacgagaatt atggacactc
    16441 gatgactaac aatatcctta ttagtatcta tccacgatac atggaaaaaa ttataaacaa
    16501 acaaaaaaac tttgaattta gaaatttcta tcttacctca gatccttata atatcttttg
    16561 gatttacgaa acaatcccca ccaaagcttt aaagtataaa ttgattgtaa aaaatccaat
    16621 tactaaactt aagaaaaatc aacactactt tttaggagac gatgagtttg aacaattagt
    16681 ttaaaaggga aaatatgctt ctatcattac aagctcgttt ggtgtaagat gggtataagt
    16741 catttatgtt cactctcctt gtatgcttta gcgggtatta caatttgagt gtaacataaa
    16801 tggctttttt atttgtctcg cttaattata caaacggcga attataaata actcataaaa
    16861 aatattttga ctttcaaaat cacttttgct accagaagtt gaggcgaagt gttttaagca
    16921 tagttgtacc ctagtttgac cgttaaaaat aaataacgat cgctagagtg caactatgtt
    16981 tgtttgccct accgtcagcg tcggcttggc ctctcacatc cattaccgtc gggattatct
    17041 ccgttacata cccttacaaa atacgccctg taagcattgc atgaaaccac cgtgcatttg
    17101 caccgtattt ccgccctagc cgttcaaatc ggtcgctacc accattcaga aacacgtgcg
    17161 gtaatggaag gtttggagga agctatccag accactaacg attgtcccta tcgtcgtacg
    17221 tttcactcag atcaaggttg gtcgtatcaa atgaaagctt atagaaacaa attgaaggag
    17281 tacaaaatct ttcaaagcat gtctcggaaa gggaactgct tagataattc acttatggag
    17341 aatttttttg gcttattaaa acaagagatt tttcacggga aagtttataa ctgtttcgag
    17401 gaattaaaat cagccatcga tagctatatc tattactaca ataatgaacg gataaaacag
    17461 aaattaaact ggcaaagtcc cgtccaattt cgtaaaacaa ccgttacggt tgtgtaaaaa
    17521 tatcatttta gagcatgaaa aaaagagtgg atttctccac tccaaaaaag tctaactttt
    17581 gggggtcact acatttttga tcctttttat ttgattaatt atatccagct ggataataat
    17641 tttgggggca acatttctat cttactatta tcttcctaaa gtagtagact ttcttattgg
    17701 gaaaaattac aacaaaaagg agtggcttag ttgacaactc ctttttgttt acaaatcaat
    17761 tggatatcta taaattgtat tttctaacga attttataga catctgattt gtcaaattaa
    17821 gctagacaat atgtctttat ctatgcagct caaaaatact tccaatggtg ttcgataatt
    17881 taatgatttt cttggaatat ggtttctttt cgaggcaacg gattgaataa aggtttcatc
    17941 cgtgtcccta aagtccattg ttttaggtaa gccatcctgc ttgagcccat gcactcggta
    18001 ctgatgagaa aaaattgatg cctgtttctc ctgaggaatg ccaattccct gatcttttac
    18061 ttcaataatc acgctaccct tttggtttga aatggtgaca attaccagtt ttccttcacc
    18121 atatttgatt gcattgtcaa ctaaattcga aaccgcccga taaatccaac gatctcgtcc
    18181 atgaatcagg gttggttgat cttcagtaaa cgcaaacaaa agattcggat aattcatttt
    18241 gtaggcatca caaacttccg cacagatcag tgaaacgtct acgacttccg tgctaagatc
    18301 ctctgagtta ttggataatg tcaaaatatc gtcaatactg tcggtcatcc gatctaaaag
    18361 cattaaattt ccttgatcgg catgttgccg ttctatttcc agattcgtac gcaaaatagc
    18421 gatggcattt ttctgctcgt gggtcagata actatttaat cgtcgataat cctccaaatg
    18481 accatcgaac ttatccttta gcttattaaa agccgcattt aatgcctcgt ccttcacagt
    18541 atttttagga acttgatcaa taaacgttaa atcacgcgta atcgctaaga tttgattact
    18601 gtaaattttg cgtaaaacca accaaagaat aaacgttaat aacatgacgg cagaacaaat
    18661 aaccagcacc atcgctggta aatatttaaa tagttcgtcc tggtaagctt tatctatcat
    18721 cgaaatagtc ataaattgat tgttggaaga tttcgaagct tccatctgcg tctctgcttg
    18781 aaccgtctta ccctcttcca taaaataaac atcttcacct aaggtagatg cgatactgat
    18841 tccctccaaa ttatttctca gaaagaagaa actcactaaa atcacgcctg tcaccaacag
    18901 tgaaaaagcc agcatcgtta acgtcatttt aaaatttaat agcatagctg gtaccccttt
    18961 gctcgaatgg tttttaagac ctctttttca gctgcttcag ccagtttctt ttttagacga
    19021 gccagatgca ctcgcagaac cgacgaaaaa ggatcaaaat cttcatcgta gacatgttca
    19081 gaaatttcct cagacgaaac aaccgctgga tgacgctgag cgatgcacag taagatatca
    19141 aattccttgg gtttcaggac cacctctgtc ttttgatagc tgacccttcg acctaatgga
    19201 tcaatcttca aagcaccaat tgaaatctgc ggcagcgcac gtccatgata acgacgaata
    19261 accgcgcgta ttcgtgcttt taattctagt aactcaaagg gtttgactaa ataatcatct
    19321 gctccaaaat ccaagccttt tgctcgttct tcaatctcat cacgtgcagt aataatgata
    19381 actggacttt cgacttcttc agcacggaga ctttgtaaaa cagataatcc atctttttta
    19441 ggcagattta aatctagtag tatcccatcg tatgaattga tgaaggcctt ctcttctcct
    19501 gactcgccat cataagctac atctacagaa atattctcct gctctaggcc cttttttacg
    19561 gataaaacca gctcacgatt gtcctcaacc accagtacac gcatgtatat tcactcctca
    19621 tacggattta gtaattacac ttatgctttt tagcatagta cacgctcttc ttcaaagtcc
    19681 acgttctttt tgtgaagatt tgaagaaatt catacattct gttaaaaaac acataattaa
    19741 gaatgatttt actgtttgaa aatacctaga atagaaaaag actggaacct ttcctccagt
    19801 ctcactactt tctatttgct tggttttctt gtatatactt ttcaaagttc gttggtttat
    19861 gaccaataag cctcgttaga atactgaaaa ttgccatcaa gtcattaagt accacatgta
    19921 agaaaatctc tctaaagaaa aaccccttcg ttttaactca cttactattt tagaattcgt
    19981 tcccctaata atgcagcatg attttacgct ttatgtataa aaaaagactg caagcaattt
    20041 tgcttcacag tctgatttct tatgatagtg agcttgttaa cacttccacc ggtgtagcag
    20101 tttctttcaa tacggcattt aattcttcaa tatttttacg cccttgcgta ttcatccact
    20161 ccatcgctgc ttcttttcca tcaacgatat agctttctac accatctgcc caagtcgcac
    20221 ggccgcaaag tacaccattg aaggttgagc cggcattttt cgcgaagcgc agtgtttctt
    20281 ggaacaactc tgcttttaca ccggcgctta ggaaaataaa tggcaggtta gtggcttcag
    20341 cctgttcctt gaagaagtta gccgcttgtg cctttgtgta cacgacttcg tctgtcccat
    20401 agccttccac ataattcata ttgacgggta cttccacctt taacacatct acgccataac
    20461 gtggatcaga aaattccttc atcatatcgt tgactttatg aggtttctta atggcgtatt
    20521 ctttcgaagt cgcatcagag atgttcgcgt cataagaaac aagctccaag aagaagggta
    20581 agttttcagc ctgacattct gagccgattc gttcaataaa ggcatgtttt tgattattga
    20641 tttcttctgc ttcatcgaca tcatagtaaa gtaggaactt acaagcatcg gcgcctgctt
    20701 cttttaaacg tttaactgac cagatcgata ataaatctgg taaacggccg ggagttgacg
    20761 catcgtaacc agttttttca taagctaaca gcaagccggc attttttgct ctaacgtcag
    20821 ctgctggcaa gccatattcg ggatctaata aaatcgatga ggcatatttc gttaattgtg
    20881 aagaaacaat ttctttaaat tcaacgattt ctgattcttc tgcttcgctg cccttgaatt
    20941 ttgtgatcat cttcttcaaa gccccgcgtt gatcaattgc taaggcccca atcactccat
    21001 cttcattgca tagttgcttg atatattgac gtttttcttc gttcatttcc gtatcctcct
    21061 acactttctc cactaagatt tgatcatata gtttttgata attggctaat tggacatagc
    21121 cagtcgtcgc ttcctgagca ttcagcattc ctaaaacatt accctgtttt aacacggctt
    21181 catccgaaag accttgttga atcgcagcgg tcaatccggc aacggtcgca tcaccagagc
    21241 caactggatt gacgacatca atctttggaa ttgtcacgca ataaaaagtc tcgtgatgtt
    21301 tggcaaaggc tccgtcgccc cccattgaaa ccactatcca ttcaatttca tcaaacatcg
    21361 aatctgacaa ggcggctttt aaatctgata attctgttga gatttctcgt cctagcagac
    21421 cagccagctc ttcggtattg ggttttatta gcttaggctt tacactgcct cgcaacactt
    21481 cccttaatgc tgtacctgaa caatctaaaa cgacaggctt tggctgacaa acttgcagca
    21541 tttgtcgata aaaatcagca ggcaatcctt ctggcaagct tcctgaaaaa gaaatgattt
    21601 cggctttaac agctaatttt tcgaaatgtt gcaagaacgc ccttgcttct tgttcagtaa
    21661 taacaggtcc gttttctagg atttccgtct gttttccgtc atgtaaaaca gcgatgcaat
    21721 tacgtgtctc tccgctgata ggtaaaaagt catgagcaat ctcattttga gtcaggtcct
    21781 ttttaatctc ttcacctaaa aaaccgccga ttaaaccact agcaatcacc tccgtatcta
    21841 attgcttcaa gatacgtgtc acatttaggc ccttaccgcc agctgttttg cgaacatttc
    21901 ttactcgatt gactgtgtcg agcttgaagg tttctaatgg ataagaaata tcaatcgatg
    21961 gattcatcgt tattgttaaa atcatttgtc cacccgctta atcgtggtat tcgccacgat
    22021 cccatttctc gataaattca tcgaagaaat gcggatcaga ctgcttgtca ctggcttttt
    22081 cgacgtgatc gattttggcg atcaattttt tgttttcttc agtttcttga taatcggcat
    22141 tgatgaaggc ctctaatatg tcacagatca aaaattctcc cgtaatccga gcgccaaagc
    22201 cgatcacatt ggcatttagt tgttccttcg catagatagc agtcgtcata tcgcgaacca
    22261 gcgcactgcg gataccaggt actttattga cagcgttgtt aatcccaaca cctgttccac
    22321 acaaacaaac tcctagatct gcttctccgc tagtcacggc ctctccgact ttttttccgt
    22381 agatcggata atgggttctg gtaaagtcat aggtccccat atccaacact tcatgtcctt
    22441 ttgattttaa gaaatctgat actgccattt ttgtatctgt gacgatgtgg tcacagccaa
    22501 ttgcgatttt catttcattc atccttccta attacgccat tttatttaac atgtcgacac
    22561 gtacttgatg acgaccacca tcataatttg cttgtagaaa ttccttaaca atattcaacg
    22621 ccaactcatc gccaacaatt tttgatccaa gtgtgatcat ccgagaatta ttatgtttac
    22681 gggtcatata cgcactgcgc tcatccgaaa cttctgctgc taccattcct ttaacctttg
    22741 ttgccgtgat aaagctgcca gcgccgtagc catcaaccgc gattcctaaa ctaccttcgt
    22801 tttcattcag atcatttgcg atcgccaaag ttgattccac aaaatcttct gaagccgttt
    22861 cactcttatc tactactgta ataccttctt tgatcaattt tttcttgatt aattccttta
    22921 attccatgcc gtcactgtct gaaccaatta ctacacgcat ttttgtttcc tcctattttt
    22981 tactgaatga ttttagtgaa ttgctgataa taactcttca gctcggctga aagagtagaa
    23041 tccgtaaata atttgtcgat cttgcgcaaa tcatagaaat tataaaaatc gctatggtcg
    23101 aatttgtttg catctgaaac caaacatttt tctgccgcat tatctaaaac aattttttgc
    23161 aaggtacctt cttcaatact gaaattcgaa acctccgaac ccttcaatcc attcactccg
    23221 acaaaagcct tcttaatacc gattttttga acagcttcat tggcaattga accgacaaag
    23281 gctccagtct tttcccgata tagcccaccg atcaaataca gttcaaatct ttttatttca
    23341 cttaatagat tgaaaaccgg cagactattg gtaacgattc ggagagatga cggcttgatg
    23401 tatcgtgtca ttaattcgat cgttgtaccc gatccaagaa aaatcgtgtc gtcttcttga
    23461 ttgatatgag ctgccgcatt tttagcgatg tattccttct cttttgtatg cagcttcttt
    23521 ttttcagtat gagaaagctc atttgcttct atagaagtcg acatttccag ataatcgatt
    23581 ttttgtgcac cgccatgaac gcgtaaaagc tttttcttct gcgcgagttc ctctagatct
    23641 cggcggatcg tcatatctga aacattcagt acttcctgaa taaatttgac tgttatcgtc
    23701 ccatagcgat cacacatcgc taagatcgaa cgctgtcgtt ctgttttcag catattctgt
    23761 acctccacga ccttataata aaacatttac gaacattttt caacacatat tgttcgttta
    23821 ttttcgttta tgcgttgaca atgattttgt aagcggttat actcaacttg taaacatata
    23881 aataaacata aacgaactaa taaaggagct gttttgtgtg aatgaattct ttttcaaaga
    23941 cactgtctat gtttctgaca agcagtcgca ggaagaagtg ttcgaggaag tctatcaaga
    24001 tctgttaaag aaagaattgg taaccgaaga ttttttggag aaccttttag aacgggagcg
    24061 taattatccg acgggattag atctctcacc tgtctctaaa atattgccag atatcgctat
    24121 ccctcatacc gaaagtgagt ttgtgcgaac cacccgtatc attccaatca aagtaaaaaa
    24181 tgaaatccct ttctacaaca tgatctcgcc aagcgatcag atttccgctt catttctttt
    24241 catgatcttg aacgaaaatg gtgaagcaca ggctggcatg ttggctaata tcatggattt
    24301 cattaattct gttgacgaag aaaaactgtt ttcgtttttc aaattagagg acacagaagc
    24361 gatttatcat tttttagaag aacattttta tatatagaag tctctgccta accgcaggat
    24421 aaaaaataaa cttattttaa ggggagaatt attatgatca aaattttagc agcatgtgga
    24481 gcaggcgtta attcaagcca tcaaatcaaa agcgctttgg aaactgaatt gaaaaatcgt
    24541 ggctatcaag tcacctgtga cgccgttatg atcaaagata tcaatgaaga aatgctgtct
    24601 cattatgaca ttttcgcaca aattgccaac accgacttgg gttttgacat taacatccct
    24661 gtcgttgatg caggtgctat tctttatcgt attcctgcta tggctgagcc tgtttaccaa
    24721 agtatggaaa gcgcaatcca atcattaaac aaataggact aaagaaagga aaagcatatg
    24781 aatacaatta ttgatattgc caataaagta tttacccccc taattaattt aggtgcggca
    24841 ccgttaatga ccatcgtatt aactgttatt gctttatttt ttaaagtaaa accgtcacgc
    24901 gctcttgaag gcgggctcaa attagggatc gccctgactg ggatcggagc tattattgga
    24961 attttaacta ctgctttttc agacgctatg acagcttttg ttgaacgcac aggtatttca
    25021 ttaaacatta cagatgtcgg ttgggcccca ctagcaacca tcacatgggg gtcgccatac
    25081 acgctttatt ttttactaat tatggtgatc gtcaatattg tcatgttagc aatgaaaaaa
    25141 accaacacat tagatgtgga tattttcgat atttggcatt tagcaattgt tggtttgttc
    25201 gcgatcttct gcggtgctaa tctgtttatt gcttctgcat tagttatttt cattggtgta
    25261 ttaaaaattt ggaactccga cttaatgaaa ccaaccttca atgacttgtt aaatgcgcca
    25321 gacagcaacc cgatgaccac gactcatatg aactacatga tgaatccgat tattatggtt
    25381 ttcgataaaa ttttcgacaa aatcttccct tggttggata aatatgattt cgatgctgcc
    25441 aaattgaata gtaaaattgg cttctgggga tcaaaattcg cgattggaat ttatttgggg
    25501 atttttgttg gattactagc aggacaaaca ccaacgcaaa ttttctcgct agcctttacc
    25561 gctgccgtct gtttggaatt gttctctttg ataggcgctt ggttcatcgc agccgtagaa
    25621 ccgttatcac aagggattac cgattttgcc aataaacgac taaaaggacg taccatcaac
    25681 ataggtttag actggccttt catagcaggc cgcgctgaaa tctgggctgc cgctaacgta
    25741 ttagcaccaa tcatgttgct agaagccatc atcttaccag gaaataaatt attgccacta
    25801 ggcggaatca tcgcaatggg tgttacccca gcactattgg tcgtaactcg tggaaaatta
    25861 atccgtatga tcgtaatcgg cgcgatcgaa ttaccattat tcttatggtc cggcaccttg
    25921 atcgccccgt tcgttactca aacagctaaa gcagtcggag cctttccagc cggcttgaac
    25981 gggtccgcca tgatctctca tacaacgatg gaaggcccga ttgaaaaatt cctaggttat
    26041 ttagtcggta atgcttctca aggacaaatc gaatttgtga tatacgctgc cttagcatta
    26101 ggtgcatacc ttttaatctt catttggtac gctcgtcaaa tgaaaaaacg caacgctgca
    26161 tacgcagctg aaaaagaaga gaatgcatct ccggcaacgg cgcataatat gacttatgca
    26221 gccgaagcta aataagctaa aaacaccccg aatgtcttta gacttcgggg tattatttta
    26281 cctataaaaa ataaatagat gtaaaatcca tttcaaaaag catagcccat ctcttgccac
    26341 acccaataaa gaaattatca acgtttatgc tttttttaac tcctttaagc ttgcctttgt
    26401 tggtattcct caacgattct ttttgtgttc ttgtttgttt tgtgcttttt gactttttgt
    26461 tgtttaattg cgaattcctt gatttctttt ttcctgaatc gcttcgcatc ccgcggttat
    26521 gcgtgcgatt tcatcattgc tttttctgat ctaactcgtt tcatgtcaaa gagcttcagc
    26581 tgacttagtg tccatggttg ttttgatatc cc
//
LOCUS       DASHFF010000034        25998 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.034, whole genome shotgun sequence.
ACCESSION   DASHFF010000034 DASHFF010000000
VERSION     DASHFF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.034"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            289..606
                     /locus_tag="U2I75_001604"
     CDS             289..606
                     /locus_tag="U2I75_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN1696741.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="U2I75_001605"
     CDS             complement(697..1062)
                     /locus_tag="U2I75_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696742.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="U2I75_001606"
     CDS             1270..1752
                     /locus_tag="U2I75_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696743.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /locus_tag="U2I75_001607"
     CDS             1949..2839
                     /locus_tag="U2I75_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEN1696744.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="U2I75_001608"
     CDS             3165..5639
                     /locus_tag="U2I75_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1696745.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="U2I75_001609"
     CDS             complement(5742..7478)
                     /locus_tag="U2I75_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN1696746.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="U2I75_001610"
     CDS             complement(7475..8179)
                     /locus_tag="U2I75_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1696747.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="U2I75_001611"
     CDS             8310..8813
                     /locus_tag="U2I75_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN1696748.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="U2I75_001612"
     CDS             8773..9111
                     /locus_tag="U2I75_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN1696749.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="U2I75_001613"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="U2I75_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN1696750.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="U2I75_001614"
     CDS             10662..11240
                     /locus_tag="U2I75_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN1696751.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="U2I75_001615"
     CDS             11244..11765
                     /locus_tag="U2I75_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1696752.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="U2I75_001616"
     CDS             complement(11844..12398)
                     /locus_tag="U2I75_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1696753.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="U2I75_001617"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="U2I75_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN1696754.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="U2I75_001618"
     CDS             12938..13189
                     /locus_tag="U2I75_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN1696755.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="U2I75_001619"
     CDS             13313..14104
                     /locus_tag="U2I75_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696756.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="U2I75_001620"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="U2I75_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN1696757.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="U2I75_001621"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="U2I75_001621"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN1696758.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="U2I75_001622"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="U2I75_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN1696759.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16339..16581
                     /locus_tag="U2I75_001623"
     CDS             16339..16581
                     /locus_tag="U2I75_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEN1696760.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            16932..17186
                     /locus_tag="U2I75_001624"
     CDS             16932..17186
                     /locus_tag="U2I75_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HEN1696761.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            17215..18474
                     /locus_tag="U2I75_001625"
     CDS             17215..18474
                     /locus_tag="U2I75_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1696762.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18527..19423
                     /locus_tag="U2I75_001626"
     CDS             18527..19423
                     /locus_tag="U2I75_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1696763.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19434..20303
                     /locus_tag="U2I75_001627"
     CDS             19434..20303
                     /locus_tag="U2I75_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1696764.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20313..20988
                     /locus_tag="U2I75_001628"
                     /pseudo
     CDS             20313..20988
                     /locus_tag="U2I75_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            21194..22858
                     /gene="alsS"
                     /locus_tag="U2I75_001629"
     CDS             21194..22858
                     /gene="alsS"
                     /locus_tag="U2I75_001629"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEN1696765.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22871..23581
                     /gene="budA"
                     /locus_tag="U2I75_001630"
     CDS             22871..23581
                     /gene="budA"
                     /locus_tag="U2I75_001630"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEN1696766.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23711..24208
                     /locus_tag="U2I75_001631"
     CDS             23711..24208
                     /locus_tag="U2I75_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN1696767.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            24393..24653
                     /locus_tag="U2I75_001632"
     CDS             24393..24653
                     /locus_tag="U2I75_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696768.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(25015..25254)
                     /locus_tag="U2I75_001633"
     CDS             complement(25015..25254)
                     /locus_tag="U2I75_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696769.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            25540..25854
                     /locus_tag="U2I75_001634"
     CDS             25540..25854
                     /locus_tag="U2I75_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696770.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         8636 a         4373 c         5305 g         7684 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    16141 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    16201 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16261 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaata
    16321 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16381 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16441 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16501 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16561 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16621 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16681 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16741 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16801 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16861 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16921 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16981 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    17041 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    17101 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    17161 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    17221 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17281 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17341 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17401 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17461 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17521 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17581 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17641 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17701 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17761 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17821 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17881 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17941 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    18001 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    18061 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    18121 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    18181 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18241 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18301 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18361 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18421 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18481 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18541 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18601 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18661 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18721 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18781 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18841 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18901 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18961 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    19021 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    19081 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    19141 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    19201 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19261 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19321 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19381 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19441 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19501 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19561 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19621 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19681 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19741 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19801 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19861 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19921 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19981 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    20041 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    20101 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    20161 aaatgtttat tgggctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    20221 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20281 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20341 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20401 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20461 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20521 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20581 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20641 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20701 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20761 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20821 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20881 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20941 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttataaatt
    21001 gaaactttca attaagaatg acagtttttt atgtaaaaac tgtcattctt tttcattttt
    21061 tgaaacatat ggctaaacaa atgaaataaa acatgctata cttttgaatg aaacaggttt
    21121 agaaactgta tcacttattt gtgattattt ctaactaata attaaaaata aagcataaaa
    21181 aggacgatgt tcaatggctg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt
    21241 gattaatcat catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt
    21301 taatgaatta gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc
    21361 atttatggca caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag
    21421 tggtcctggt gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc
    21481 agttttagca attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag
    21541 catggataat gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc
    21601 agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg
    21661 cgcgagcttt atcagtatcc cgcaagatgt agtggatgca cctgtccaag gtaatgtcat
    21721 caagccattg agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc
    21781 tgaacgcatt agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga
    21841 aaaagaaacg ttagccatca ggcaattagt tgaaaagaca gcattgcctg ttgtagagac
    21901 attccaagca gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg
    21961 tttatttaga aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgcaat
    22021 tggctatgat ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat
    22081 cattgtgatc gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt
    22141 gatcggtgat atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt
    22201 gtcagaagat gcaaaagaat atttagcatc tcttcaagca aaattaacgg agcgggacat
    22261 tgttcaatca aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca
    22321 gtcaaaagta acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat
    22381 ggcacgccac tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac
    22441 gcttggtgtt gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacagat
    22501 cgtatcagtt tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt
    22561 acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga
    22621 gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt
    22681 tgtgaaatat gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt
    22741 agaaaaagct ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat
    22801 tgattaccgt gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg
    22861 aagtgtcgac atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc
    22921 cggcttgatg gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt
    22981 gggaacattg catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg
    23041 aagatcagat ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat
    23101 tacagatttt acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt
    23161 gaaaaaagac atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac
    23221 tggattattc aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag
    23281 actagcaaaa atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt
    23341 agtcgggttc tttacgcctg aattattcca aggagttgct gcagcgggat ttcacctgca
    23401 ttttatcgat gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg
    23461 aaaagttgag attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac
    23521 ttttgttcac gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata
    23581 gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga
    23641 gaaaacctag cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc
    23701 ggtttttatc atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca
    23761 gcaacaagga ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc
    23821 attgttgaat gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa
    23881 tgaattgatt ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa
    23941 aggagtttgt ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac
    24001 ccatgcaaat tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt
    24061 aaaagaaggt aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga
    24121 tgaaatcgat caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat
    24181 aaatgctttt tcatttatta ataactaaaa tcgtctattg aaacaaaaaa gaagagaaat
    24241 caagaataaa aagagccgga aagacagtgg gcatttctgt ttttcggttt ttttattttt
    24301 ttataaaatc acaagtgtta ttgattttca taatcatatt ttaatgttat tatcatttta
    24361 aataatgaaa aaacattaag gatggatgaa atatgaataa cgaaactatc attttaggaa
    24421 aaaaatatat ttgccaacct attggcttga aacaaaaagt gattggagaa gtaatcagta
    24481 aattagaaaa ttgtgttgtg atctgcgtag acgaatatca tgacgtcgat cgcaatgaaa
    24541 tattagaaaa atgcggaaaa gtagtggcaa aatatactca cgtttatgag ttggaaacaa
    24601 cagaatatct atataactat agcaacaaat atgaacatac tttagtttca taaagattta
    24661 aataaagtgc cttcaaaagc gagaagacca agcttttgaa ggcactttat ttagttggca
    24721 gtgattgttc atgatttttg atttattcaa aaggttcgcc tagagaatct gtctctgtaa
    24781 cgaggcctgg tggagaaact cgataaaaac ctatactgcc agaactgtcc tattttcgga
    24841 tagacaaact agtagtttta aaaagatcat ccgatccaat catttcaaaa aatgcaaaac
    24901 tgatatcagg atcatttagc tcaggcttgt tatcgtaaag catttgtaaa ggttgcattt
    24961 agttaggagt aaacgcgaaa ctatcatcat tatttgctga agaagatgaa gaagctaatt
    25021 cagagtttgt ttgaaataaa ggagacgtgc tgacagtttc tttaccctgt tttttgcaga
    25081 ggtagaaact tttatatcgg aagtttctac cggagaattt ttctcatttg tagaaattgt
    25141 tgaatcgata ctattcgaaa tttgcgtatc tgaaggtatg ttctttgatg cagaagtccc
    25201 acaagcagta agtaacaata aatttgataa actgataaac agaattcttt tcattatgta
    25261 cacactcctt atagtatcgt ttagcataca cctatgctca gtattaggca aaatcaatgc
    25321 aaaactatca agtcttttat agtatctatt agtcttagta atacatatat ggagagggtg
    25381 atatcccagt tcaaaccaga tacattggtt accatctgta ttaacgatta tgcctctaaa
    25441 cgcttttttt atgaaaaaat ttataagtga aaacgtaggt atgatattat ctagaggtaa
    25501 tttttttggg tatttaaata gttgctagga ggatagcttt tggattttta cagtaatttt
    25561 atcttaataa tagcaatatt attacttcta aatatttggt tttttgataa gagtagaaat
    25621 gcagggatag gttttagaac caaacgaagt acgtcttctg aaaaaaagtg ggtatattca
    25681 cagactattt tttacggagg ggttatttca atcagtttac tttcttcaac actttattct
    25741 tttaatgtta tagacgtttc catgtcaaat tttatatcta ttattggaat ccttatttct
    25801 gcaattatta cacaactact tcttgtgttt gaagagaagt caaaaaataa ttaaagcaat
    25861 tatgcctttt agtacaggaa ttattacttc ttacaaatgt atagcgaatg tgaagcctgc
    25921 tgttttaatt gttctagaaa aacttttgag tgaacaatta ttttatgtta ggatgaatta
    25981 atttctctag agtttata
//
LOCUS       DASHFF010000035        25297 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.035, whole genome shotgun sequence.
ACCESSION   DASHFF010000035 DASHFF010000000
VERSION     DASHFF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.035"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(114..629)
                     /locus_tag="U2I75_001635"
     CDS             complement(114..629)
                     /locus_tag="U2I75_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEN1696771.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(811..1422)
                     /locus_tag="U2I75_001636"
     CDS             complement(811..1422)
                     /locus_tag="U2I75_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1696772.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            complement(1448..2899)
                     /locus_tag="U2I75_001637"
     CDS             complement(1448..2899)
                     /locus_tag="U2I75_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN1696773.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            complement(2923..3654)
                     /locus_tag="U2I75_001638"
     CDS             complement(2923..3654)
                     /locus_tag="U2I75_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1696774.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            complement(3672..4067)
                     /locus_tag="U2I75_001639"
     CDS             complement(3672..4067)
                     /locus_tag="U2I75_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEN1696775.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            complement(4169..5806)
                     /locus_tag="U2I75_001640"
     CDS             complement(4169..5806)
                     /locus_tag="U2I75_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEN1696776.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            complement(5841..7175)
                     /gene="gmhB"
                     /locus_tag="U2I75_001641"
     CDS             complement(5841..7175)
                     /gene="gmhB"
                     /locus_tag="U2I75_001641"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEN1696777.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            complement(7217..8326)
                     /locus_tag="U2I75_001642"
     CDS             complement(7217..8326)
                     /locus_tag="U2I75_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696778.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            complement(8351..9586)
                     /locus_tag="U2I75_001643"
     CDS             complement(8351..9586)
                     /locus_tag="U2I75_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696779.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            complement(9617..10747)
                     /locus_tag="U2I75_001644"
     CDS             complement(9617..10747)
                     /locus_tag="U2I75_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1696780.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            complement(10734..11507)
                     /locus_tag="U2I75_001645"
     CDS             complement(10734..11507)
                     /locus_tag="U2I75_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1696781.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            complement(11521..11976)
                     /locus_tag="U2I75_001646"
     CDS             complement(11521..11976)
                     /locus_tag="U2I75_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HEN1696782.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            complement(12398..13504)
                     /locus_tag="U2I75_001647"
     CDS             complement(12398..13504)
                     /locus_tag="U2I75_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1696783.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            complement(13522..14283)
                     /locus_tag="U2I75_001648"
     CDS             complement(13522..14283)
                     /locus_tag="U2I75_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEN1696784.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(14359..15357)
                     /locus_tag="U2I75_001649"
     CDS             complement(14359..15357)
                     /locus_tag="U2I75_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN1696785.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(15376..16029)
                     /locus_tag="U2I75_001650"
     CDS             complement(15376..16029)
                     /locus_tag="U2I75_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1696786.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(16039..17049)
                     /locus_tag="U2I75_001651"
     CDS             complement(16039..17049)
                     /locus_tag="U2I75_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEN1696787.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(17068..18318)
                     /locus_tag="U2I75_001652"
     CDS             complement(17068..18318)
                     /locus_tag="U2I75_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEN1696788.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            complement(19664..19827)
                     /locus_tag="U2I75_001653"
                     /pseudo
     CDS             complement(19664..19827)
                     /locus_tag="U2I75_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
     gene            complement(20180..20869)
                     /locus_tag="U2I75_001654"
     CDS             complement(20180..20869)
                     /locus_tag="U2I75_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1696789.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(20914..21678)
                     /locus_tag="U2I75_001655"
     CDS             complement(20914..21678)
                     /locus_tag="U2I75_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN1696790.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(21706..22404)
                     /locus_tag="U2I75_001656"
     CDS             complement(21706..22404)
                     /locus_tag="U2I75_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN1696791.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(22417..23196)
                     /locus_tag="U2I75_001657"
     CDS             complement(22417..23196)
                     /locus_tag="U2I75_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN1696792.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPIQPKATNDKTAPSRRSRSRV"
     gene            complement(23212..24156)
                     /locus_tag="U2I75_001658"
     CDS             complement(23212..24156)
                     /locus_tag="U2I75_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1696793.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(24197..24976)
                     /locus_tag="U2I75_001659"
     CDS             complement(24197..24976)
                     /locus_tag="U2I75_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696794.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
BASE COUNT         7799 a         5433 c         3701 g         8364 t
ORIGIN      
        1 tatctatatc taaatttagt tttatgtatt cctcatagaa ataaccgagc atttgctcgg
       61 ttattgttct tcttattttc cagtatcctt cttttctcca cagctcattt taatcaaaag
      121 cttaatcttt ccacattttt gtctttctca cttataacga aacactgccg actgaccggc
      181 cattcaatat ctacgctttc ataacttata catttggcag cattagaatt gaaatcatta
      241 tctacgcacc actgaactag cgtattgtcc tccatagtaa gatagccatg agcatatcca
      301 cgtgggatat acgcacattt accattgctt tcactcaatg tgttgacata cacacatcca
      361 taagtggaag atccttgtcg aatatcaagt gcgatattca taatttcacc atgtaggcac
      421 atcactatct ttccttgagc atgctctcca ctctgataat gtagccctcg catagtatat
      481 ttattatgac taaatccttg atttatttgc ttaatgtgaa actcaatacc aatcgagaaa
      541 agagtctcat tatccaatgc aacagcagta tatcctctga aatccgaaaa cttaattata
      601 tcaaacactt ttaccataac taccaccatg ttatatattt taccaatttt ttaattcgct
      661 cgacctcatt taaagcaata aaattcccta cctcttgaag atataacaac aatgttttag
      721 caaaaataga tggcatgtaa agtattcttg atgagattac acaatcctgt ctgcagaaaa
      781 atattaacca tattaaaatt caaagcagtt ctattttaag tctatatact tatttaatcg
      841 gtagaatagc caatatagca aagcaccaat taagccccct agggtgttat aaaacaaatc
      901 cgatagttga aatgtaccaa gcctaaaaaa cagttgtgta aattctatac ttaatgagaa
      961 taaaaacaca tacttaatcc caacatagat ggtgcgtata atatcacatt taatgtcacg
     1021 ccaccataac aataacatgc aaaaagggat aaacagcatc gtgttctcca cgcattctgt
     1081 tgtcaatctg ctctctcctg tagcattatc tataatatat aatgaccact gtccaataac
     1141 gtttgaaagc gggttcatcc acatatttcg attaagcaag gttttaaata atatcaaagt
     1201 aaaataaaac gcaagaaaaa agagtctgcg aaatttacta gatgctttaa aatatctcaa
     1261 ccagattaat acagaatctt ttaagttttt aggatctcga gagttatcaa ctataaatag
     1321 ataccagtac ataaacagat acgctgttaa tacggcaaat aagaacggct gatataatgc
     1381 tgttaataca tttaccgtta tagcagaaat aatatctatc attctctatc tctctatgag
     1441 caaatgttta tttgctcata attctcctta ctaaaagtag caatcgtttt ctaaatataa
     1501 ttgcagcgac acaagcaatt gctataatta aatattttat gaaatcaaat ttattaatta
     1561 ttgaaattag gatgccacca gctatcgtga ttgcagaaat caaaagtatt cctctgacat
     1621 catatatgtt ttttccatcc aatagttctt tgcaaacttt tctataaaaa atataatgga
     1681 tgacgcagaa aaacatatat gagaatacag ttgtgtatgc taccgcctga tacccatata
     1741 actttataaa aacataattt agtacaatat tcataattgc acacaaaacc gatggaataa
     1801 cgacagtgag tttcttttca aagtattcct gtactctagc aaacagctga aacatatagt
     1861 tgaaataaat accgatacat ataggtacaa ttaaattaac actgtcgata tatttctgcc
     1921 caccaaatat caacacaatt tctttactaa atagcattaa tgcaaaaagc atagccgcta
     1981 ccaaaccaag aacttcatta gctctttttg atagtaaatc atattctcct gacttgattt
     2041 tttgatactg ataaggtgca aaagtggaat tgacagaatt tgtaacaagg ttaattagag
     2101 caccaataga atatgccacg ctgtaaatag caacatctga cgaaccacat aagtaattta
     2161 tcataatttt atcagaacca ttcagaataa attcagataa atagtgtggc atcaacgata
     2221 cacaaaatac tactgcgaaa ttccacactt cattatcctt aaaatcctta catcttctaa
     2281 ataccgatat atacacagcc acgccaacaa caccagtcac taggacattc attaaggctc
     2341 tcgcctcagc ggtaggagat ataaacagaa ccgctaaaca gccaactaca gatgagccaa
     2401 cagtaactgc aagggtagct actaataaag tcttataatc atatatatat cgtttgtgta
     2461 aagaccaaca ttgtatttct gcctgaaata taaaatatga gaacatacag acaactagaa
     2521 ctggtgtcaa tcctaaaatt tctgccaata tatccttaat aaaaatagca ataacaaacc
     2581 aaaaaatagt cagtactaaa gtcaatccgg atactgtcga aagaatatgt tcttgattat
     2641 catattttac ataaagattc atcatggctt tgaacaaact aagcgatgta atcatgagca
     2701 ataaggattc ccacgataga taagtgctgt ataaaccgta ttgttctgta gacaaaactc
     2761 tagtaaaaat tggggttgta atcattgaaa cccctctctg taaaaaacca cataccataa
     2821 accataatgc agccttaaca ggaagtggag tattcttata tctgcttaat gtttttacaa
     2881 ttagattatt actggtcact gctattttct cctaaacact atttattctt gattcaaaaa
     2941 cacattgagc tcttctggag taccaagcac atgaaccttt ttcgcatcta ctatgctaat
     3001 agtcaccttt aacccttttt ttatcataaa gttatataat ggtgctatat atttttcatt
     3061 cttctccata ttagaggagt ctgcataata ctcttcatat aaagctttat acagctttgc
     3121 cgatgagaaa taatacgcac caagagtaca attatcagaa atgcgaactt tttcgcggac
     3181 ttctattact tcatttcgat cgttaagctt tgcaaaactc caatgatcgc catcagcatg
     3241 aaagcatgga atatgtccat ctccacaaat atcttcgtat ttcatctcat ttgattccac
     3301 gtatgtatca atattgtaaa ccataatctc attgtcttga ttgcaataag gaatagccaa
     3361 catacatgta gttgcttgac catctgtcat atagtctatt tcaatcactt ctacgttttc
     3421 aataccaaag taagcacaat gctcctttat aaaagctgaa gcgttgtctt cctgacgaac
     3481 aacaaaaata tacttatcaa catttttatt gtatccaacc aaactatcca tggcccaatc
     3541 aaataaagtt cttcctttgg cattaatcat atatttagga accgcataac caatttttcg
     3601 aaaacgactt cccaaacctg ccatcgtaat gataactgtc atcttttttt ccatagaact
     3661 aacctcctta tttagaatct tttttttgaa ctgttgcaga tttcatttaa ttcatctaca
     3721 gagcatctca aaaactcatc aggtctaacc gttcgatcat ctacatagaa gcctttatgc
     3781 cccggccaag gcttaccata caggatttca tcatacggaa tgtcccattt atccaaccac
     3841 gccaagagca cttttgcagt attagcattt atcattccca aatttccttt ataggaatta
     3901 acattgcgtg aggtgaacaa tgtaatttta gcaccattct ccttatagaa tctcagtttt
     3961 tcaaccatat tctcatacgg caccaaatcc tcatatcgct catccttctt ctttatcgga
     4021 cacaaagtgc catcgatatc aaacacaaaa gaatattcac tatacatttt tctcttccct
     4081 tctattgtat taattaatat aatcgtgcct taatgcctac attgctaaaa cagtattgta
     4141 accgttattc attaaggcac aatttatatt agagtataac tttattactt actcttgcaa
     4201 gaagttccat acctgtagca agcatcacta tttggtgatc aaaactttca ccgtgtaatg
     4261 gaatcatact aagaaatagc aacgcttcta tcaatacaat cttatcaaaa tcgtccttaa
     4321 tctcgtccga aaagaccgac ttaaacaatt caaataaatc gtaatctctt acacgcgtat
     4381 taattgtata atctatcact gcacactcgg tatcatattc tacagtaaat agatccttga
     4441 tgatataatc atatttacca tctaaactat gaaatagttt tgctaattca tatctgctgt
     4501 ccccataaat atcatatttt ccaaattttc ccctaggatc aataacctta acaaaggaac
     4561 aattggaatc taccatcata ttagcaaaac acaaatctcc atgtataata caaaattcat
     4621 ttacatcata gagcatactt gggataagat tctttagaag ttcaattaca ccggttaaag
     4681 aaataaactt acgaccatta atttgaattt gtttctcaaa aaaagctgaa aatcttttat
     4741 cttcagacaa tttttgaaat ctctgcatgg tttttcgcaa atacatatct tccaatgact
     4801 ctctaatctt ttcatctttt aaggtgtatc gcttaaaatc gctacagata aactttatac
     4861 ggctaaacag atcatgccac tggttatatt ttaaatcacc atataagtat aactcatgaa
     4921 ccgtatgata tgcataatat tccatatcaa catagggagc tacgtaagat gtagaataat
     4981 caaaaattct aggtcttaca tattcaatat cagcaggaag ttttaaatac cactttattt
     5041 ctccaataaa tttatccttg tcatcgctag ttttcttcaa tatccctcga tctttatcta
     5101 tagagatatg attaaactct cgtgctttaa cttccaactt tgtattgtaa tatttatcga
     5161 tgtgtccaat atcaaaccac ttttctgttc ttacggcctt tagcggacaa cgttcgctgt
     5221 atagctgtaa agcccgataa aatgagttta ttcccccctg tttccgactc tcaactgcct
     5281 ctcttaagca actcttcaga agttttgacg aagaaaacat aaatacgcca ataaataatt
     5341 tttcacgttt atcggatacg taagcttcct ctttatcgtg gatatcagta attattcctt
     5401 cattaatttt aaagtatgtc caagtactcg acacaaaatc ttcagaataa tagaatgtat
     5461 ccatttctat atcagacata ctctccataa caattgtatc tgcgaagtta attagaacag
     5521 gctcatcttc atctgataac ccaaaataca cggtttgccc tagatcccct aattcaggaa
     5581 ggtcacatat atgaattctg gaatcatgat agtttttcag tcttctatga accttatccg
     5641 ctttttcata acagataata tccaatgaat cgcagattgc attatactga ttatacaaat
     5701 aatcaaagac aattttttta ttaattggat agataattgc tggtaacatt cctatactct
     5761 gcaattcttt ttcaacaact tttgctgctg gaattaatac cttcatctaa ttactccttt
     5821 tacaagtttc actaaaccaa ttattgcata gtgcataaat gatttttaac aaattctaac
     5881 aatgaacaga cactatagtc ttggccataa ttagcaggca aggtgatgtc ttctgtaatc
     5941 agtactgtct tacaaccagc attttttcca gcaattacat cactttcact atctccaacc
     6001 ataaatgatt gacttaggtt tatgttaaaa tcctttgatg cttctaataa cagtccaggt
     6061 tttggcttgc gacaattgca atcaattttt aattctataa cctctcctgc atatccttta
     6121 tggggatggt gcggacagta ataaataccg tttaaaaatc caccagcatg ccctagcatt
     6181 gtttcaagct tattgtgaat actattaagc tcatcaacag taacttcacc tcttgcaatt
     6241 acaggctgat ttgttataac aatacacaga aaacctagag aattaataat tttaattgcc
     6301 tccgatactc catcaattaa ctcaaattct tctgcatttc tcaaaaaacc aacgtattta
     6361 ttaatcgttc catctctatc taaaaagatt gctttttgtg gattggatag atttttacca
     6421 acaataattc ccttttgaaa gtccttaatt acagctctat atcggtccgg tgttcccata
     6481 tctttaacat actctggaga atcgtagcaa tacatacgcc ctgtgttagc caatggtttc
     6541 agaatcattc tatctaaatc aattttgtat ggtagccccc caccttcaac tattgattta
     6601 tttacaattt ccattaatgg attttctctc aacaccttcg gatttataac atgaagacct
     6661 gcatttactc ggtttttata atattcaggt ctttgatctt ccttcgctaa ccattgaagc
     6721 actttcttat tgtcatcgga tataattaat ccactatcat acggatgtga gttaggatgc
     6781 gtaaacaaag taacatctgc gttattctca acatgaaatc taatcatctg cctgaaatca
     6841 acatcaaata ctgcatcagc attcaaaagt aaaaacggtt catcacccag ttcatccaac
     6901 aaaaaataca atgcacctgc attgccaagt ggagtttttt caataaaata tttgatttta
     6961 attcctaatt ttgaaccatc tccgaaatgc tcctctattt tttcatgcaa ataggaaaca
     7021 gtcaaaataa tatcattaaa gccttgatct ctaagcgaac acaattctcg ttctaaaact
     7081 ggaatgccat ctataggaat cattggctta ggaatatccg gaaacaattc cgaaattcgg
     7141 cttcctcgtc cgcccgccat aattactgtt ttcataatat gcccttccct aaagtacttt
     7201 ttactataaa agttaattac tcttcccaaa caaagtccat atatttttca tattctgctt
     7261 tgtattcgta ttttccagtg tacaaattca accaatttat aaaatcaaaa ttatagcagt
     7321 gaagtgcatc ttggctatca tcaatataat acgtaccata aatagtatta tttgaataac
     7381 gacctacata tttatgccag tacaaatcaa tttgattttt gttaatagta ataacatggt
     7441 tttttacaaa ctcccaatat gccctaatag agcactcctc attgcccccc attcgtgaaa
     7501 tatagcttcg aagaagctga acttctggag ctaaattatt ttcagatatc tcccttcttg
     7561 agactcctct agacacagct cccttttctc tcacatcact aggaatcgag aagaggttta
     7621 tcatatcatc aacatcacca aaatacagga agtctgacaa acaatacggg aaaaacatgt
     7681 ctccatacgg cattgatagt aacagtaatc gctgattttg tattaatttc caatcatctt
     7741 cgttattaac aggatactgt tcaagtaaac tgcagaaaaa cgccattgca tttggatggt
     7801 atattctttg atctgttcta gttttacaaa catactttgc accaagctgc tttgctctga
     7861 ctattccagg caaagtgttt ccaacttgat aattaatatt taagtgtcca ggcttttcag
     7921 gtttctcact acaaataact ataactccaa gatcctctat ccttttcaat gccttaatat
     7981 cttcgtcctt ccatgtcgaa agaataatta aagcatttgg ataacatttc ttatagtagg
     8041 ccagagtatt ataagagaaa tcatccttta aacatagtgg cccctgcatt acaattgcta
     8101 catctttgca acaattaact aaacccatca ctggaacatc agacatgtcc tttgagcgca
     8161 attgaaacgc tgcataaact tcagaatttt cttctactgt tgcacaatca gcagtcaatt
     8221 tccaagatac gagtttactt atattatgac caaacatgtt cctaaacaca tatggtaaat
     8281 tttcgtactt cttcaatatg ttatataaaa tctctttctt attcatgaga tgcactccta
     8341 ttaatatttc ctattctatg ttagccacac ttctacattc ttgatagatg tattctataa
     8401 ccgctaagca tacaggaata gctggagtaa atatcggata aaaccagtct atattaatcc
     8461 ctgttagcaa gacatttaat gtaattccaa atgctaaata ttgatagcca gtcataccca
     8521 cttttgatgc aagtaatgct gttttgaacc cttttataat aaacagaata tcaaatataa
     8581 gtatacctac accatattga gtaattgtat agaaaaatcc aatatcagat aggtgtcctc
     8641 taactcctcc tggtaatata aacgtaaatt cctcaccagt aagaatacca gtaccgaaca
     8701 aaggcgattg caaaaattta ggaataaaaa accaaactga agcaaatcga gattcagcag
     8761 atcctgcata ttcacctgta ctgtttgtga aagtttcaac aaatgttgag aacatggtcg
     8821 aattaataaa tatgactaca agcaacccag ctaaaaccca cattgcaatt tttcttttcg
     8881 aagaaccttt cttaaaaagg atatagcaac caagtacaat aatttgatag acaaggacag
     8941 atctagaacc atgaataaca gcagaatatg ccagtgcgct agcaatcagt aaataatatt
     9001 ttacttttct tctagctaca acttgtcgct caatcatata caaagcaatc ggtattatta
     9061 taattgtaaa acatggtgga ttaattctta aaaagcctcc acgataccaa ttttccgggg
     9121 cacactcacg atatatgttt tcatagagaa gtactcctga aaatttctga ataagtgaaa
     9181 tagatgttct aagaatgtat gaaagaaatg ttacaccaca tatcgtattt agcattgaat
     9241 ccaaattcca cgcttggtta attaaagtac agaacaatgg atacgccaat aatacatagt
     9301 ataaactcat actttcccta gatttaaccc atattgtctt atctgagtag atgtaagctg
     9361 ctattataac aacacaaatc agtaaagtgc agaaatttaa atatctattt tttctaaatg
     9421 ctggtaatga ggctattaac aaacatacta ttgctagaat gactgtcaca agtctaacgt
     9481 tcatcgtttc tcccaaccag ccgcccaaag gcttcaatat gcttgatgag ataatcatta
     9541 gaaagaccat tagaaactgt atgattttcc atttactaat ctgcatgtta attattattc
     9601 ctttctaatt gttttactac tcattcaaaa tttgtttgta tgcctcgtat gtgagttttg
     9661 cagatgccgc ccaggtatac ttgcggttta caagctcata tatatcatcc tctattttgc
     9721 ttttctcagc tgggctaagg ttcacatact ttacaattgt gtctgctagg ctcttagaat
     9781 tcaaattttc gaaaagtaac cctatctttc cttcctcggt ttcttccata aaagcaggta
     9841 cattactaac aatagttggt aagcaataag tgtatgccat aagaagcgct ccacttccat
     9901 agatttcttt atatggaaat attgcggcat ctgcatttct gaacatactg tccatttctt
     9961 catcagaaat tctctctggc ttatatagaa cacaatctga aacctttaaa tcttcaatca
    10021 tttttttata gtcagtacta tcctgtttag gatgttgttg acctgctatg gtaaaatgaa
    10081 ctttctttct tacatccatt ggaagttctg tgattgcatt aagcagtaca tcaattccct
    10141 tatatggacg aatttgtcca aacaagataa attcaaactt atctttatca tacgctctag
    10201 atatatgttt aacatctcca tagatagcat gtggacaaac atatgacttt gcatttaatt
    10261 ctgggaattc agcatataaa tgctgttctg ttactcgatt gtgaacaatg aaaccatcac
    10321 aatttgaata tatattaaag tgctcttggc gatccttttc acgaacctca tgtggtaaca
    10381 cattatgaac tgtcataatt aattttttat agtgtctctt acataaaacg tacaatccgc
    10441 cttcatattt gagacttttc atcaattgca aatgaacaat atcataatca ccaaatctta
    10501 tttccttcca aattaagaac aagcttttca gaaaaacaag tgccgaaaac actttatttg
    10561 tacttttatg atacaaaata tgtttgctct taattttagg atctaaagga ggaacatcac
    10621 gcttacacag caatgttaaa tcaacatatt gattaagttc tttagaaaga tcaaacgaat
    10681 accaattctc tggcatttgg ctttcacaca tcaatacctt cataatactc accttataac
    10741 caatcaacag aaaattctgc tttcagtttt cctttaattt tacgttttgc tataaacatt
    10801 tccagtatct ctcgcatttt cgtctgatat tttgtcgttt taccataaag cgagtgattg
    10861 tcccctgttc ctaatacata tactgctgct ttgaatggaa gaggttttaa tggacgccct
    10921 tgctcttcac atgctggaac aacgccccca tgtcgttttc taatgatcca tggattatca
    10981 tcacaatttg ctgtcataac ttccggatat gcctcaggca ggtcttccac tgcatatttt
    11041 actatacacg cacttcctga tgagaacttt ggtagaacct tcatataatc gtctccaaca
    11101 aagtacacgt atcccgtttt gacataaaat ccattttcct ggggatggga attcacatat
    11161 tccgccaaat gagaacttac aagatcatct gcgtcaacca tcatagcgaa accgccgccc
    11221 aactctcgca aacgcattgc gagagcatgt gttttataac ccttatcaat cattttttcg
    11281 tcttgatttg tgggaatagg ggcatccttt tcgctaactt gaataaattc aactctgtca
    11341 tcatattcat gcgtgagttt aggaatttca tggcaagcaa caatgatttt gaaatcagga
    11401 tcagtctgat tataacacga ccataatgtt cggttaaata aggtactaac catctcccaa
    11461 ttgtttgctg ccttcgcact cattaaagga ataacaaaat acaacataca atctctcctt
    11521 ttagtaacga ttcaaactac aataatcatt tacatcaata tcttttttca atactctagc
    11581 cggaacgcct cctaccgaac aattaggttc aacatcatta atgaccacag cattagcccc
    11641 tactattaca ttgtccccta ttgtgatatt acctaaaatt tttgcgccag tagcgatata
    11701 tacattgttt ccaattgtag gcataccagg cttattggat ctaccaccag tcagtacatt
    11761 cgttccgatt ataacgtgat tacctatttt tgtatctttt ccaattacac aacctatgcc
    11821 accgtataag aacttacaat tatctccaat ttctgtttga tatgaaattg agctattatg
    11881 aagtacataa attagtgcct ttacaatctt ggcaaaaaac tccattttgt gtaagtaaaa
    11941 ccatcgttct actttgtata atttatatgc attcatgttt tatctcccca tctttttagc
    12001 aggtaccccc cacacttcgt agggtggaat agacctcaat accactgtat ttgcagcaac
    12061 tacagcacct tccccaacgc ataattctgt gttagattta caaagaattt tagcacccac
    12121 acataaaaca gcgttctttt ttactcgaat gatgatacgt tcattactat ctgctcaacc
    12181 acacgtcaca ttctgataga ctttaacacc atcttcaagg attgtgtcag aatgaatcac
    12241 agtgcctaca gaattatgaa caaattcaac agagttccct atctgtgcac ctttaccaat
    12301 acaacaacca agaattttgg ctatttttct cgtgcaatta tcaactatgt ttaaagcata
    12361 tcgattcctc aatgccatta atttcgttac ttccatatca tttatcacct tccaacatgc
    12421 taagcataat ttctcccgac tttgaccatc taaattctgt agttacacgc tgatatgctg
    12481 catctatgat atcgttggta aatcgtccgt cttttaagaa tgtcaaaatt tcatcgtcag
    12541 atttagccaa cattccatat ctattatcca aaataatatt ctgtgggcca ggtgcattaa
    12601 cagcaataat cccgaggcga tagtacattg cctctagcaa aaccataccc caaatttcag
    12661 tatagtttag atttacaaat acatccgata agcaatataa ctcccaaata tctttgttag
    12721 gaatacgctc aattattttt actctattct gcagtccaag taaatttatc tgctgcttcg
    12781 tttgctcgag taaagcacca gttcctacca tcagcaaata attatgctca tcaagtttat
    12841 taaataaact aatcatatct aacggtcttt tatccttttc aaaccgtcct acatataaaa
    12901 taacacgaac attgcgagga atattccatt tttctctgag agtagtaaaa tctattgctt
    12961 cgtaattctt atgtaactgt tcaaaatcca cacaaacagg agcaagtttc actttattta
    13021 ctcctaattg tactaatttt tcagaaactc gatcattttt ggcaacacac ccatttttct
    13081 tataaatttt ataatttcga tgcgccaata tttcaataat tgtctttata tatttattgc
    13141 cactttgact tcgcgttatc ccattataag gtatatattt tattccattt tttacagtcc
    13201 atgagtatac atgaggtact gatagttgaa ggtccgcaaa ttgtattaga acatccgcat
    13261 tcttatcaaa attctttgtt gataagaatc catttgatcc tacataacga cacttgatat
    13321 agtgaatcat cgccgtattg cttaattgct ctgttccaac ttcctcatca ctttttacac
    13381 ttttatatac aataacttcg tggcctaatt cagctaaccc tcgagctaat cctatttcct
    13441 gagaattata ggaccccttc ttaccaaagt tattcgtcat tgtaactact ataagtattt
    13501 tcatctattt ttattgcccc cctacttaga ttcaatagta tagtagatat actttatacc
    13561 tcccaagata taccgcctcc atagtctacg aggttccttt accagtctaa ataaccactc
    13621 tagactagct ttttccatcc attcaggagc tttttccaaa gtgcccgcaa aaaagttaaa
    13681 gccggcgcct acaccgaaca ttactgtgtt atggatcttc ttctggtgtt cccacatcca
    13741 catttcttgc ttaggcgcac caataccaac ccacagaaaa tctggattta agttgttgat
    13801 ttcctcgcaa atcttgctat cctcttcttc atttaacgga cgaaatggtg gggaatacat
    13861 tccaacaatt ttgattcccg ggtaatctct ttctaggttt gcacgaagct tatttaacac
    13921 ttcgttatct tttccaccat agaaataatg tgtttttccc tgtctaacgc cttcttcaaa
    13981 aaccattccc ataatatctg gtgcagcaca tcgttcacag gtaaatcctt tgcgccgtcc
    14041 aattttaaca ataggcatac catctattgc agccattgta gcctggctat acatttcagc
    14101 aattttggga ttttcctttg ccgaaataat ggccggcact cccacgagat caacagtcgc
    14161 acttaccgga gatgttggca tggaaaaaac ttcccttaca aactcgttct ttgtagtagc
    14221 acaaacacct actattccac acacagtgaa atcacgtttt cttttataca tattcttttc
    14281 catctcagta catccttact catatgttct tcctccgcat cctctaatta cacggaggaa
    14341 gaacagctta tatacttatt aacccttaat ctgcttttca atccaattgt aggattcacg
    14401 aagaccctct tccaaagaaa cagtaggctc ccaatccaaa atctctttcg ccttagaata
    14461 atctgcgcaa cgagccttat caccttcagg cttagaagta tcaaagaaag gcttgatgtc
    14521 tttgccagaa atttcaatga tcttatatgc aatttccttg atggaagtac agtgagaagg
    14581 gccaatctga atatgaccat gaccccaacc cttatccagg gcgagcacca atgcatttac
    14641 gatatcattg acatgaatga aagcacgtcc ctgctctccg ctaccccaca cattaaaagg
    14701 ctcttcagga ttattgattg ctttacgaat cagagcagga ataacctgac tacgttcgcc
    14761 aaaatcagta ggtgtgccat aaacattatg gagcatcagg atacagcacg gaattccggt
    14821 ttctttttcc aaatacccca tctccaactg gcccatcagt ttgctccagc cataagcaga
    14881 ctcaggaaga gcagggaaca actcctcttc acggagaggc tcaggattca aagtattctg
    14941 tctggtcaga gggaaggagc atacggtacc aacatagata acacccttga ttttctcctt
    15001 gccagcctta cggcaacaat caaaaacatt agaattgatc agattattga ttcggaacaa
    15061 ttcgccctgg ttcttaaaca catagtcaat gccagccaca acatcagcca gatgaactac
    15121 atactcagtc gtaccaataa cctttttagc ctgctcataa tcagtcagat cagcattgaa
    15181 gaaatgagta tccaggtcga tgacaggatg cccatcttcg tcgttcaggt actccaactt
    15241 tcctctccac agattatcag ctacataaac atcccagcag ccttccttca ccagtctctt
    15301 aacaagattg gagccaatca taccgcaacc accggtaacc ataattgtct ttttcattta
    15361 acattcctcc aaattttatt cgtttccaaa gaatttatct tccagcatca aacaccaaca
    15421 atgatatatc ggcaagtgat actcttgaat catgtatgtt tcaacttcag gagccttaac
    15481 acaaacatct gctaccgtgg caagttcccc accctttgcg cccgtcaagc caataacttt
    15541 tataccgaga gccctagcaa caacagttgc gctcataata ttcttgctat ttccggacgt
    15601 cgaaatacca aggaatacgt caccagatct accaaagcca aacagctgct gtgcgaagac
    15661 acccaaacca tcgacatcat taatataagc agtagacaat gcttcgtgtg cgaccaaagg
    15721 aattgccgtt aaaccacact cgagattttt ggctaattcc ggacctctta cagggtcaat
    15781 tgcaactaac ttatcggcaa attccttact tacaggacgt ggcgtcttaa accgcttcat
    15841 caactcgcca gcaatatgtt ccgagtcagc ggctgatcct ccgtttcctg ccacaagaag
    15901 tttaccacca ttcaaatagc attcctccat aattaggtat ccatcgataa tgtcctgttt
    15961 aatcgtctca agtgtaggat atcgtttaat caataagtca atatgtttat taagtttttc
    16021 cgctaacatt gttactcctt atccattctg tttttcaata taagtttccg gcgtgtagtg
    16081 aataactcta gttccaccat tttcaaattc aaacggaata tacatcaaat cacgcatagc
    16141 ccaacgaact gcatcctgtt tctcaggttg tacatagaaa accaagaaac ctccaccacc
    16201 tgcaccgagc aactttccgc ctaaagcacc tgcagccata cccttttcat atagttcatc
    16261 aatgcttcct gtactaattg ctccaccagt accttttttc aatctccaag taacatcaag
    16321 ctgtctaccg aaatcatcta aatttctgtt cttatcagta agaattgcct cagcttcatc
    16381 tacaagctca tacatctttt tcagtcttac tctcttatcc tctgtgccac tgacgttgtt
    16441 agccttttga acatcagaag aaaatctagt gaatccggta aagaacatca tcagattgtt
    16501 gttaagctgc tttttacgtt caggcgaaat aatgacaggc agcacctcat aaccatccac
    16561 attgaaatta attcgattga acccaccata agatgcggca atctgatcct gccatccacc
    16621 ggcttcatta cacagaactc tttcaagata aatggcttca tcagcaagtt tcttcttgtc
    16681 agcatactta ccctccagtg cataaaaagc attcaacata ccaactgcaa aagaactgga
    16741 tgtacccaga ccagagcgag caggtaaatc agcttcgtat gtaagccgaa tttcatgcat
    16801 atcgagcatt ttcatcgcat tacgaatcgc aggatgctca atatcttcaa tgctagttac
    16861 tctctcagta cgagaataag ataactcagc gctgtaatca aaaaatctag gcagatgtct
    16921 cacattcaca taacagtact tatcaaaagt agtagaaaga actgcaccgc cattctcttt
    16981 gaaataatcc tccatatccg ttccaccgcc aaaaaaagac atacgaaacg gtgtttttgt
    17041 aataatcata aatactctct cctaatctta gtctctctgg aagatatcac gggtatatac
    17101 tttttcctta atgtcatcaa ggcaagcatc gtagcgattt gctataacag aatcacacat
    17161 tgccttaaac tgttccaaat cattcacaac cttactgcca aagaaagtct ctccatcttt
    17221 caaggtcggc tcataaatca caacagttgc acctttagct tttacacgct tcataacgcc
    17281 ctggatagaa ctctggcgga agttatcaga attggatttc atggtcaaac ggtaaacacc
    17341 aatcacaaca ggcttttctt tgccagcatc atactgattt gcttctgcgt aattgtaata
    17401 accagccatc tgaagtacac gatccgcaat aaagtcctta cgagtacggt tagattcaac
    17461 gattgcctcg atcagatttt caggaacatc tgcgtagttg gccagaagct gcttggtatc
    17521 cttcggcagg caatagccac cgtaaccaaa ggaagggttg ttgtaatgag taccaatacg
    17581 aggatcaagg caaactccat taatgatctg ctgagtgttc aagcctttca tctctgcata
    17641 agtgtccagc tcattgaagt agctgacacg aagagccaga tatgtattgg caaacagctt
    17701 aacagcttct gcttcagtaa agcccatgaa caatgtgtcc acattctctt tgattgcgcc
    17761 ttcctgtagc agtgtagcaa aagtgtgtgc atcctcaacc aaacgctcat catccatatc
    17821 ggtacccaca ataatacgcg aaggatacag attgtcatac agtgccttac tttcacgcag
    17881 gaactcaggg ctgaaaataa tattcttgct accagtcttt acacgaacag aagcggtgta
    17941 tccaacagga atggtggact taataaccat aatggcatca ggatttactt ccatgaccag
    18001 cctaataaca gcctcaacag cggatgtgtc aaaatgttga gtgcggctat tgtagttagt
    18061 aggagccgca ataacaacaa aatccgcaga ctggtatgct gcctttgcat ccaatgttgc
    18121 ggtcagattc agttctttct cagccaagta cttttcaata taatcatcct ggattggaga
    18181 tttacggtta ttaatcagtt caaccttctc tggaatgata tccaccgctg taaccttatg
    18241 gtgctgtgcc aacagagtgg caatactcaa accaacataa ccggtgccgg cgactgcaat
    18301 gttatactgt ttcttcataa tatttcctct ttccaccact gctgtggtca ctactttctt
    18361 aacaaaatgc tgtttgtgaa cagtaacctg ctatttatat aaaacagccc gaaagctgtg
    18421 ttcattcata cttaaaacag ccagctccta cgccggcaaa ttcaattttt cataaatccc
    18481 ctacccgttt agggttatat tgcaagtttg agtcattttt attgacctcg tttggggtta
    18541 taatacattt tcgcattgaa atgtacctac attttccact caaccagtca ccaaaaagtt
    18601 tataaattca cttattaccc tctattctta ctccaaaggt atgtttaaca tcgctttatt
    18661 gacctatttt caccccaaat ggaagtttgt ttggtacatt attggtctat tcccacccca
    18721 aactgtctat tttgagcgag tttttgtttt ttatcattgc acattttcaa ggctcaaatt
    18781 ctaagcggat ttactggcag ggagacattg gtaaatactt gaacaatttc acggctttca
    18841 tcatcacatc ctacgataat aattctaatt ggataaagat tatcattaat aatctacttt
    18901 cacgtaaaaa ttctgaacta aataaaatct attgataata tttttttctt ccactttttg
    18961 tatatcctac aggtatagtg gatttaatta tccccaatgc ctctgaagta acagacatca
    19021 ctaattcaat aacacattca ctaccatagt ttgtaggaac aaaaatgaca acaaaatctg
    19081 cttccttatg tgatgttttt ccatctgtcc aaattttatt accaatgaaa tctagtttaa
    19141 aatccctctt agaaactagt tcactctata tttttattca gtttcattag ttctgttttt
    19201 ttgtcttcag tcaccatata catatatatc ttaattatct atccatttca tcctacgcat
    19261 tcttgcgtaa atacaattca ctctgttgag ctgatataaa tcattggtgg tacaaagata
    19321 aaatgataac tgtttctata cctccaaaaa ttccagtaaa tatatctata gatagtttca
    19381 aaaaaaattg tcttagtttc ataatattta agatagtggt acatacttta taacaacgta
    19441 cttttccaaa tattattttc cgttatgaac atccaatata ttacaaagtt taaaagttta
    19501 gatgaactat aatcttcatt tctgcacagt ggataataaa ttcttataga taaaaaatag
    19561 cctcttctaa agtcacaaat accttactcc cacattttaa gtaaacactc tgcagcattc
    19621 tctttgttat aaaatgatag atattcctca attttatctc ttattatgtt attatcagtt
    19681 gataccattc cattaatgtt tcagaataat tacaatattt tgggaccgga taactccctt
    19741 ttgctcgtaa ccactcatat ccgttaggat tcactaaaag tttaccgttt atttttcgat
    19801 tctttttttg aaccgtccca tgaacatacc gattcaacaa gctaaattat aaaaattggt
    19861 gtgctgtcat tattttttgg aaaatttatt aatggctatc aatatacttt tgtatcatac
    19921 taaatagctc gtcagttcct atatcggata taccaatatt aaagcattta gatcattata
    19981 tttaagagtg gtatcatccc aaaatttcga atattttctt ttatataaga ataatgatac
    20041 tgaattgccc cattcacctt tcttttttta gattttctac aagtgattca aaccctccat
    20101 aattacagtt attacttttg aaacgataat atgaacatgt gtaatctttt ttattaacaa
    20161 tccatcattc tatccctaac taatatgctc cattgggctt gacaataaca taaaacgttt
    20221 ttaaaatcaa cctcaagtcg tacattaaac ttcgatgact aatatactct aaatccaact
    20281 caaccatttt atggaaacta acattatttc tatcactaac ttgccacaaa cctgaacaac
    20341 caggttttac atacaagcgt tgaagatcat atctcgtgta ctcagctact tccctctcca
    20401 aaggtggccg tggaccaatt agactcatat cgccttttaa aacattccat agctgtggca
    20461 attcatcaat gcttttggca cggataattt tacctatttt agtgacacgg ggatcttctt
    20521 tcatcttgaa cattgcacct tctatttcat tttgatcaag taattcttcc aatttttctt
    20581 cagcatctaa acacatcgaa cggaatttat acattttgaa tgtttttcca tttttaccaa
    20641 cacgtgtatg gcaaaaaaat atcggacttc ctggctcttc ttttctgatt cgataagcta
    20701 catagaacat caaaggagaa gctaaaatca aaccaataat gcttcctaca atatcaatca
    20761 ttcttttaaa aaataaatag atatatcgtt tttcaacttt ttctttatcc aaccaaactt
    20821 gattaggatg tacttcacta atacgagata ataaatcttc actttccata ctttccctct
    20881 ctcctcattt cctttttatc tacgaataaa gaactaaaat aaaccaaact tcttcttttt
    20941 tacctcttca tagactggat aactgatttc atccccgttg atcagatcct ttgcgacttg
    21001 tttcatctgt actacttttt ccttaccaaa gtccttagca atctgctcat agcactcttt
    21061 taagtaaaag gtacgcttag agaccccatg ggcatcagaa gcagccatct gcacaagatt
    21121 atgtttgacc atcattttgg ctgttttctg gatgtctttt ccaaatacac cgacataact
    21181 tggtgcggtg agttgggcca agcatcccat atctaggaat ggcagcatat gattcgggtc
    21241 tttacgaaaa tgcgcgttgc gttctggatg gacgatcact ggtgtctttc ctagttgtaa
    21301 caactcaaaa aagagctgtt ctgcatataa cggcacttcc agtgtcggaa attcgattag
    21361 aagatatgta tcatctaaat ctgtaaataa aatttcatct cggtcgatgt cttcgatcag
    21421 tgttccggta atacgtactt cttgtccttc caataacgtc aaaggcaatt gtcttttctc
    21481 taattctgct tgtaacgaag cgactagaga aatgacttgt gatttttcat tgctgtattt
    21541 tccattatta tgatggggtg tgcataaaat atgtgtgatc ccttgttgaa tcgctttttc
    21601 agccatggcg atactggcat ctatgttttc tgctccgtca tcgattcctg gcaggatatg
    21661 gcaatgtaga tcgatcattt ctcttgtctc ctctcttttt atttcttaat tcccgtagta
    21721 atagccagca tctttgctgt gttctgcccc attgtataca acaccaagga ctcgcgcttg
    21781 taccatatcc agcaattctt ttgctttagt caatgattct ttccgtgaca tattttcacg
    21841 tacgaccaat aaggtgccat ccgttttgga agacatgatc tgtgcatctg ttacggcaac
    21901 tactggcggc atgtcgaaga tcactacatc aaatagttgt cttgcttctg ctaaaatctg
    21961 atccattctt ggcgaactta gtagttctga tggatttggt ggtttaggtc cacttggcag
    22021 aatcgacaaa ttctcgactg gtgttcgttg gatctcatct gctacacttc ctgatgaact
    22081 aagaacggta cttaatccgc ttgcgttgtt caacttgaac gttttataga tgacgggctt
    22141 acgtaagtct gcatcgacta gcagcacccg ttgaccagat ttggcaaaaa caacggcaat
    22201 gtttgctgca gtggtcgatt tcccttctcc aggtcctgat gaagtcacaa caatcgtttt
    22261 aatttgctga ccagctgcgg aggcaaactg gatattcgtc cgaatcgtac gatattgttc
    22321 agcgattggt gaagaggggt ttgtgagtgt gaccaaactg actgcatgag tttgggctgc
    22381 tttttgtttc tgtgttcgtg ccatttttct ttcctcctag actcttgagc ggcttctgcg
    22441 tgatggcgct gttttatcgt ttgtagcttt tggttgtatc ggagctggtc cttttttacg
    22501 gataccagca ttcaattctt tcgctgacat ttctggtaca gtccctaaga tcgggaagcc
    22561 caatgtatcc gtcacatatt tttcatcttt tactgtccgg tcgagtaatt caaataagaa
    22621 agctagacct acgcctacca tcattcctaa cactaagcca atcactagat tcaatttatt
    22681 atttggcgaa acaggtgtcg tattggcaac tgcacctgag atgatcgaga ttttatcgac
    22741 attcatcacg tctttggcgt tctcttggaa tacttgtgac gttgtattag cgatttgttg
    22801 agccgtcact gcatttgtgc ttgttgcttg gatcgagaac atttgtgaat tttgtgactg
    22861 attgacggag atggcatctt tgatttgtcc agcagtcatt tttacattgt cttctgtttc
    22921 taaacgatct ttgacttcat ttagtactag atcactgacg atcatatcct tatacgtatt
    22981 gatcatttgc aggttcgtat tgacatcatt gacatttgca gtatctgatt gcggcagtgt
    23041 cacgatcaat tgggcttgtg agctgtattt tggcgtcaag atgaaaaacg tcacgatgcc
    23101 tgagatggcc aagccagcaa acattgagat gaggattgtt atcacgtgtt tgcgtaatgt
    23161 ttcaaaaatt tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt
    23221 ctctagattc atttgatctt ttaattcttt tttcacacga tcaagttctt gatcgctgac
    23281 tttttggtat gagatattgt cttgcatgaa gccttctcct tgcagctgat cttgcttgat
    23341 cgtaccaaat gcatcccgat aatctaaagc aatctttttc atttcatcaa agctcatgtc
    23401 tgttttcata ttcgactcta gagcattcaa aacggattga tagttgctta caccgttcag
    23461 actcaacact tttttcgcta caccttctac gatctttctt tgacgttctt gtctgccgta
    23521 gtcaccattt ggatcttcat aacgcatacg agaataagac aatgcctgat ccccgttcaa
    23581 tgtgatttta ccgatcgtaa aatcatagcc atcttgtgag aaggtgaggt cattattgac
    23641 ttcgatacca ccaactgcat caacgagttc ttttagacct ttcatattga ttgaaacata
    23701 gtgatcgatt gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc
    23761 gaatgcgtaa gcgtggttga tcttgtcttt tgtgtcatgt ccaacgatgt cgacatacgt
    23821 atcacgagga atgctgacaa tcgttgtctg tttgtcttca ggactgactg tggcaaccat
    23881 catcgtatct gatcttcctt gatcgacccg acctaaatct cccgtatcaa tccccatcaa
    23941 tagaacagag aaagattctt gtttgtccag atcaactggt tttgttcgtt ctccttgggt
    24001 tcgttccact gattcgtaag ttttttcaat agaatgtgag agatctagat ataattttgc
    24061 tcctactcct acgattgcta atactaatac gacaagaatt gctaaaataa ttagaaatat
    24121 ctttttccct cttgatcttc ttcttcgttt tgccatagtt tcctccgttt gctagccttg
    24181 cttgaattct cgcttattaa tagctcgtat tgctatagcc catttgctga gcgactgttt
    24241 ctgcttttga cgcgtatgcg gctcttattt tttcattccg gatctgttcg atgagactga
    24301 gtgcggtcaa atattgatcg taagtagcag tagctgttac ttgaccgtct ttcagcatcg
    24361 tatcgaatag ctgatctagt tgcgtgactt gcgcgatctg agtatcagcg aatcctagat
    24421 attgcataac aaccgttttc caaggactat ctttaaaaaa gctcatattt tcacgtacac
    24481 gagcgacatc tgcacttgtc accttttctt tgatgacgac atcgtctaca gcttgctgcc
    24541 agtttgatac atttttagta aataaatcgt tgacagctgt ctggatcttc agttttgatt
    24601 ctgcatcctc taactgcgtt aagactgctt tcttttcttc ttggatcgca gcagcttctt
    24661 taggtaattc tgattccttg atgtcataat catctgccga aagtttgata gaagatagtt
    24721 tatgtgacaa atttgaaact gtttcttctg ttatttcagg atttaggaaa attttttctt
    24781 ttgtactgta cagcttatct acttcttttg ataaagaggt cacggtgtct ttttgattgg
    24841 tgatggcaga ttctgcgtaa ttgatccgtt gatgatgttg ccgctgatta ttgaaatggt
    24901 atccaaacaa gcaaatcaca atagcgcctg tgcctaccgc cacagtagcc aataacttga
    24961 cgaacggctt ttccatagat tcactcctaa atattgatga tagataaata aagatgtgat
    25021 tcattcccat cagaatgagc gctttttatt ttaaaagcat aattaaacta atatattcat
    25081 agaaatattt ataaacaggt tttccctata taatcgtttt cttgactagg gaaattccta
    25141 atttaaaagt aaataaaaca gtaacaagat catgattaat cgttttcatt tttatttcct
    25201 ctcttttcat gagcattttg aaaattcgaa caaagaataa aataacaaaa attattaaaa
    25261 tagaattaat aacagctatt tatatttata acaaaaa
//
LOCUS       DASHFF010000036        24934 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.036, whole genome shotgun sequence.
ACCESSION   DASHFF010000036 DASHFF010000000
VERSION     DASHFF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.036"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(369..1487)
                     /locus_tag="U2I75_001660"
     CDS             complement(369..1487)
                     /locus_tag="U2I75_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696795.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1958..3265
                     /locus_tag="U2I75_001661"
     CDS             1958..3265
                     /locus_tag="U2I75_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN1696796.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3384..4583
                     /locus_tag="U2I75_001662"
     CDS             3384..4583
                     /locus_tag="U2I75_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1696797.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4610..5878)
                     /locus_tag="U2I75_001663"
     CDS             complement(4610..5878)
                     /locus_tag="U2I75_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN1696798.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6041..6687)
                     /locus_tag="U2I75_001664"
                     /pseudo
     CDS             complement(6041..6687)
                     /locus_tag="U2I75_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7095..7598)
                     /locus_tag="U2I75_001665"
     CDS             complement(7095..7598)
                     /locus_tag="U2I75_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1696799.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7721..8485)
                     /locus_tag="U2I75_001666"
     CDS             complement(7721..8485)
                     /locus_tag="U2I75_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN1696800.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="U2I75_001667"
     CDS             8592..8867
                     /locus_tag="U2I75_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN1696801.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9583..10536
                     /gene="yidC"
                     /locus_tag="U2I75_001668"
     CDS             9583..10536
                     /gene="yidC"
                     /locus_tag="U2I75_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1696802.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10640..11867
                     /locus_tag="U2I75_001669"
                     /pseudo
     CDS             10640..11867
                     /locus_tag="U2I75_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12177..12398)
                     /locus_tag="U2I75_001670"
     CDS             complement(12177..12398)
                     /locus_tag="U2I75_001670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1696803.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12559..12957)
                     /gene="tnpA"
                     /locus_tag="U2I75_001671"
                     /pseudo
     CDS             complement(12559..12957)
                     /gene="tnpA"
                     /locus_tag="U2I75_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13171..13537)
                     /locus_tag="U2I75_001672"
                     /pseudo
     CDS             complement(13171..13537)
                     /locus_tag="U2I75_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13551..13836)
                     /locus_tag="U2I75_001673"
                     /pseudo
     CDS             complement(13551..13836)
                     /locus_tag="U2I75_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13842..14036)
                     /locus_tag="U2I75_001674"
     CDS             complement(13842..14036)
                     /locus_tag="U2I75_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696804.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14036..14512)
                     /locus_tag="U2I75_001675"
                     /pseudo
     CDS             complement(14036..14512)
                     /locus_tag="U2I75_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14980..15333)
                     /locus_tag="U2I75_001676"
     CDS             complement(14980..15333)
                     /locus_tag="U2I75_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696805.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15461..16000)
                     /locus_tag="U2I75_001677"
     CDS             complement(15461..16000)
                     /locus_tag="U2I75_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696806.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16030..16221
                     /locus_tag="U2I75_001678"
     CDS             16030..16221
                     /locus_tag="U2I75_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696807.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16263..17129)
                     /locus_tag="U2I75_001679"
     CDS             complement(16263..17129)
                     /locus_tag="U2I75_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1696808.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17153..18805)
                     /locus_tag="U2I75_001680"
     CDS             complement(17153..18805)
                     /locus_tag="U2I75_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN1696809.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18810..19712)
                     /locus_tag="U2I75_001681"
     CDS             complement(18810..19712)
                     /locus_tag="U2I75_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HEN1696810.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19796..21109)
                     /locus_tag="U2I75_001682"
     CDS             complement(19796..21109)
                     /locus_tag="U2I75_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEN1696811.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21177..22121)
                     /locus_tag="U2I75_001683"
     CDS             complement(21177..22121)
                     /locus_tag="U2I75_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEN1696812.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22158..23171)
                     /locus_tag="U2I75_001684"
     CDS             complement(22158..23171)
                     /locus_tag="U2I75_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696813.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23251..24297)
                     /gene="gmd"
                     /locus_tag="U2I75_001685"
     CDS             complement(23251..24297)
                     /gene="gmd"
                     /locus_tag="U2I75_001685"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEN1696814.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            complement(24569..>24934)
                     /locus_tag="U2I75_001686"
     CDS             complement(24569..>24934)
                     /locus_tag="U2I75_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1696815.1"
                     /translation="QPLTEVEIFHTDRGKEFDNQAIDELSTTFDINRSLSHKGCPFDN
                     AVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNRLRLHGTLGYETPVGYRNQR
                     LAQRILDNELGCANASEAV"
BASE COUNT         7093 a         5643 c         4749 g         7449 t
ORIGIN      
        1 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
       61 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
      121 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
      181 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
      241 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggaaaa
      301 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
      361 aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga
      421 tccaaaataa acactttccc gccattaatg atcacatcat ctgctaatgc attcaacact
      481 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
      541 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
      601 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
      661 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
      721 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
      781 ttttctggta atgcaaataa ataaatcggc cattgttcag gattttcgaa agaatccttc
      841 aagtacgtat caacagcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
      901 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
      961 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
     1021 tctggtagag atacttcttt tacttgtttg ttgctgacct catacaattt catggagtcg
     1081 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
     1141 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
     1201 taggtctggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
     1261 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat
     1321 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
     1381 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
     1441 ccttctgaag tcaattgtgt caataattct ttatcctttt ttgccataaa cgtacccctc
     1501 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
     1561 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
     1621 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
     1681 gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
     1741 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
     1801 acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaacttccat aacgcgcgtt
     1861 cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa
     1921 tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct
     1981 agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt
     2041 gcaagctgaa gatacgttca aagtaaatgc aaaagcagct tttgctgtag atgcgcaaag
     2101 cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa
     2161 aatcatcggt ctttacatcg tccttgatca agtaaaagaa ggaaaactgt catggggtga
     2221 taaagtttcg atttcagatt atgcagaaaa tttgagtatc accccagatt tatcgaatgt
     2281 tcctttgcat aaagaaaaca cctacactgt caaagaatta ttcgactctg caatcatcca
     2341 gtcagccaat gcttcaatgg tggccttggc agaaaaaatc tctggcagcg aagctaaatt
     2401 cactgaacga atgaaagaac aattgaaaga ttggggaatc aaagatgcaa caatcgttaa
     2461 tgcatcagga ttgaacaact cttatcttgg cgaaaaccga ccagaaggca ctggagaaaa
     2521 cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt gcccgccatt tgatcttgga
     2581 tttcccagaa atactggatg tatcaagtac tacgacccaa atgtttggcg aaaatacgca
     2641 atcaccagtg gaaatggtca actggaactg gatgctccca ggattcgtta attacaagga
     2701 aggcgtggac ggattaaaaa ctggtacgac tgagcttgca ggtgcttgtt tcgttggtac
     2761 gatcactaaa aacgatcaac ggatcatcac tgtcgtattg aatgcggacg gccatgcgga
     2821 aaacccgagt gcccgcttca atgaaacaga ccgtttgatg gactactgct atgacaattg
     2881 gtcagaaaaa gagctcggaa aagctaacgc aagtattcct agtctaaaaa ccattgatgt
     2941 aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa agccctgtaa aagtatgggt
     3001 acgaaacgat atggatactg gcaaattaac gatcacgcca acacttgaca agaagcaagt
     3061 cactgacaat gagatccaag cacctatgga taaaggtaca aaaatcggta ccgctacgat
     3121 caccttggca gacgataaat taggctattt agaagacaac aatgcaccat caacagagat
     3181 cattacggat aaaacagtgg aaaaagcgaa tatattcgtg attggctggc gacgcgtcgc
     3241 agaatttttc ggcaacttgt tttaattgaa caataagaaa aacatctttc catcggaaag
     3301 atgttttttt gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg
     3361 tataagaaaa aggagttgaa tttatgaatc taaccagaga tctgatcttt ttagttctct
     3421 atcttattgg ggtattctct atcgtttatt ggcgaaaaca gaagaaggat ccttccttgt
     3481 tcttttacgg cagtacgtgg acatttacgg taatcttaac ttattttgtc gtgttggaaa
     3541 ttttttattc gaccttttat tttttgattc cattgttgtt ctttagtatc tttgcttttt
     3601 cttatttcac agaaaaacga aggctactga atggtctttt attcaatgtt ttactcatca
     3661 gtttcggcat ctacttgttt gtcttgctgt atgaaacgca aaatattttt cttggcggac
     3721 tgatcgcctt gatcacgatt ccattgctac ttgtcctttt atttggtatt tatggattga
     3781 ttgtttttct tttttggaac ggggtcacag ttttacgtag agaatcgcat tcattagcta
     3841 atcttttaac gttgatacta gccatttttc ttacactgtt tcttgttttt gactttttct
     3901 tgttaaaata tttgccgcaa tggataaatg ctttgttttt ctgcgttccc ctgatcttga
     3961 tctatctatt tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc
     4021 ggcctcgata caatcaagac ttcatcgttg tgttaggcgc tggattgatc aatggagaaa
     4081 cagtttctcc tcttctagcc aagcgaatca ataaggcaat tgctttttat cgggctcaaa
     4141 gccgtgctac attaaatccg cccatcttat tgatgtctgg cgggcaagga gcagacgaaa
     4201 aagtaccaga ggccattgcg atgaaacaat atgccatgga acaaggaatc cctgaaagag
     4261 acattcttgt cgaaaccaat tcgacaacca ctttggaaaa catgctctat tcaaaggaaa
     4321 tcatggatca gcaaatgaaa gggccatacc gcgcgatctt tagttccaac aattaccaca
     4381 tttttagagc cggtctttat gcgcggcaag ccaaattgaa agcaaatggt attggtgcaa
     4441 aaacagcttt ctactacttg cctaatgcgt ttttaagaga atacctcgcc atcttgatga
     4501 tgaacaaaaa aagacatatg atcatttgcg gattgctttt cgcaggctcc gcattcttat
     4561 cgctgattac tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt
     4621 ttttttcttt cattctctta actattggca aaaagagtat cccttttttg accgctactt
     4681 gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag cagatcataa
     4741 agaagtatac tgccgcttgt gaaccgaaca attcgatccc catcacaaag caggcaatcg
     4801 gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga aaaggaacag
     4861 aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca aacaatggtg
     4921 tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat aacttcccaa
     4981 taaaatcata gacatgtgcc tgtccattaa atgattcttg aagtaacgga agactcaatc
     5041 cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac aactacagct ccacctatcg
     5101 tatttcgaca aacaggatga ggaatccatt gtgcatatcg cgcttttaaa aagacgatcg
     5161 aacggctgaa tacccaacca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga
     5221 acagcaacat actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat
     5281 agctttctgt catgaaattt gcaaacagtg cagcaaagaa gctagggaag atcgcttctg
     5341 ttcggacttt cccgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc
     5401 caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc cgttctgatt
     5461 tatccaatcg gaacaaacgt gccgtattat ctgctaacgc tccccccatt tggactgctg
     5521 ttccttcacg gccaacagat cccccaaata aatgggtagt tatggttcca aacagtgtaa
     5581 gtggaataag ccgcaacggg attttttctt ctccaccatt tccttgatcg atcactaagt
     5641 tgtttccacg actagaaagt ccgccgtgat acgcatagag ataagcaaaa agcgcaccac
     5701 taactggtag tagacaaagg agccaaggat gtgatagtcg gatatctgtc acaactgtca
     5761 ggctatttaa gaaaaaagca gacaagctcc ccatgaatat accgagaatc gctgtgatca
     5821 ccagccactt cagcatatac actaaaatgc gtactggact cggtaattgt ttttccataa
     5881 aaaattcctc ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc agactttagt
     5941 aggcgtcatt agctatcttt cgttagcatt taaaggcgaa caccatcacc tattcacttc
     6001 cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttct ttctttacac
     6061 gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat tggtcactat
     6121 catgacaaat ataattcccc tttttcgtat acgtgccatc agaacctgct ttcgttgtag
     6181 aaagaaaaag cgaaacattc gatgttttct gacagattgc acagattccc ttatttatcg
     6241 ttggtgataa agtaccgcta atccctttta atttttcatc ttcgtaatag accatgaatt
     6301 ttttctgcgt acctggatca ttccagccga tgtacgtatg ttcttgcaag tcgatttcat
     6361 tccaagctgg ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc
     6421 taggctgcat aaacggctga acgatttgct tcagctcctc caatgctctt tctgcttgcg
     6481 cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt atcgattctg gcagttcgtg
     6541 atctaatatg tctcgaatct tttcagctgc tagtgcctga actgtttgta ccgtttttgg
     6601 atcattgaca gatgagtacg catttaataa ctgttctact tctcttatta taaaaaaata
     6661 ttggtatggt tttattgttt gttccatgat tttaaagtcc tttcttctaa ccaaagtgca
     6721 tactttgtag aaaaaagtga tcttctgcaa agtatgtgtt acctgaagat cctggactct
     6781 tttaaataag atacgttttg catcttatca tcaccccctt taatgcttat tattctatgt
     6841 acatgattca ttcgataaat aaaagtaaac cgtatcttga acagatagtc aactgcaaac
     6901 agaaaaaagg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaatg aatggtgttc
     6961 aaaagcttgc atctgcggta ataagccgaa acttcacaaa aatttgagaa gtaattttcg
     7021 taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaat atggtctatt
     7081 attggtttta cctgttaaat cgaggattct ttcccaaagt aacgttgcca tctcttacgc
     7141 atacttgcag acatgggctg agccgcttct ttgactgcgc agtatttgtc cacgaatgtc
     7201 acaatatatc cttctttata cttaggcggt gcgatcgttg caccccacat gtgcttcaaa
     7261 atgatatctc gctcaagatc cgacaaatcg gtgatttttt ctgcattctt tacagcaatt
     7321 cttggatgga tatacgcatg tgtcccctcg tcaaatttcg tcgtacgcca atcatagtag
     7381 aacagatcat gcaaaagtcc agcacgcgct gttgctttag cattgccacc ccattttttt
     7441 gcgagcagat aactatagta agaaacactg atggaatgct ccaaacgagt ggaatgaaca
     7501 tgctgtgtgt aattcgctaa ttttttcact gcttctgttt ctaaaagatc ttgtacataa
     7561 gacatatatt cttcgtcttc acgccatttt tctgtcataa ttatcgattt gccacctttt
     7621 tgaatttcat tttcttctga aattgatcta agtataccac cagtgttcgg attattctag
     7681 tcgctctaga taaaatagct aaagcttaat agttccttaa tcataaagaa aaatgataaa
     7741 gaacgatccc tgctgcgact ccgacattca atgattccgc atttcctttc attgggatat
     7801 atacgttctg atcagtcgaa gacaagattt cctgacttac tccctgacct tcgtttccca
     7861 tgattatagc gaaatcctct gttttttcta ctttgtctaa tgcatgtgct tcttcattta
     7921 attctgtacc gtacaccgta aaccctttgc ctttcattac agggatcact tctgccaaat
     7981 ctttttgtaa cacaggcaaa tgataattgc ttccctgcat gcttcgcagc acctttgtac
     8041 tataaatatc tgccgttcct ttacctaata aaacaccagc cataccaaac gcatctgccg
     8101 tacggatcat cgttcccaca tttccaggat cctgtacatt atctagtaaa agccagcctc
     8161 ctgaaaaatc tggcgtatct tcagatgtta acgtcatcac tgctaagatc ccttgcggtg
     8221 taggtagatc tgacatagcg gtcatgacct catctgaaac tagaaaagta gaaatctctt
     8281 tgtttacagc cagccagtct ccccattctt tcatacctct ctgtgtcaca aaaagttctt
     8341 ttatttctgc atcagacgac gcagcttctt cgatcagatg gaacccttcc aaaagatatt
     8401 ctgccttttc ttctctgtat ttctttttat gtagtttttt taattctttg attcttggat
     8461 tttttactga ttggatctct ttcacattcc tcacctcaac tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg atttataaaa caggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg acgtgtccag ggcgtgggat
     8641 ttcgctatat gaccaagatg gttgctgatc agttaggcgt gaccggaacg gtaaaaaacg
     8701 aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga cgacatcatc caaaaattta
     8761 tcgaagaagt caagaagtct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagatccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtttttgaa
     8881 gcgctttgcc tcgagtatta aggagaaaca ttttccaaat agcttctcat attttgaaat
     8941 gtaatgactt aataccgaga ggcagctgat aaaacaccgt caataaggtt gtgaaaaagc
     9001 tggtctgcat caagcattaa ggactggttg tgaaaaatag ctctccatat ttttcaacag
     9061 gctggactta atgccgtaga tgcaagcttt tgaacatcgt cgataaggtt gcgaaaaagc
     9121 tgatctgcat caagcattaa ggactggttg tgaaaaagtc gcttagtttt gagtattaag
     9181 gaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttaa taccaagaaa
     9241 ctgacttttg aacatcgtcg ataaggttgt ttttgaggca ctttgcctca agtattaaag
     9301 acaggtagta aaaaagctga tctataactt gcaataaatt tgcttaaccc atattttcca
     9361 gacagaacaa aaaagttttt agttttttaa ataagaaaga aacacttgga atcactcttt
     9421 tctttctaag tatttctttc ttgatttttt gtcttttttc aaaatttttc gctacgaaaa
     9481 agaacgaata tttatatgaa atttctatga agttgattga attccatgcg cttttttagt
     9541 atagttaagt ttgtgtaaaa attatagaag aggaagtatt ctatgaataa atggaaaaac
     9601 tggttattgg gatccggact tgtcagtatc ctgctctttt tatctggctg tgtgcggatg
     9661 gattcaaacg gaaaccctga cacttcagga atcgtttatc gagtactcgt ccatcctatg
     9721 ggccaagcga ttacatatct agttgagaat ttcaattggt cttacggctg ggcagttatt
     9781 gcaatgactg tgatcgttcg tatcatcatc ttgccactag gtatcagtca atcgaaaaaa
     9841 acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac aagttgaagc agcacaacaa
     9901 aagctaaaag ctgcaacaac acgtgaagaa caaatggcag cacaagcaga aatgcagcaa
     9961 gtatatcgcg aaaatggatt gagtatgacc ggcggaatcg gctgtctgcc tctattgatc
    10021 caaatgccga tcttctctgc cttgtatttt acggctcgct acacagaagg tatccgtgaa
    10081 tcatcttttt acggcatcga tctaggctca ccaagtatgg tgcttgtggc catcgcaggg
    10141 ggcgcttatc tgcttcaagg atatatttca acaatcggta ttcctgaaga acagaaaaag
    10201 acgatgcgta cgatgctgat cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg
    10261 ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc
    10321 atcacaaacg ttataatgaa accgcgtttg aaagcacaag tggcagaaga gttgaaacaa
    10381 aacccgccaa aacaagtcgt tactccaaga aaagacgtga cagaatcagc agcaaagcca
    10441 gctgacaaca aacaagcaac aaaacaaatc aaacaaccaa aaaacagtac aaacaacaaa
    10501 ggccgcaacg caggaaggca gcagaagcgg aaataaaaat atatgacaaa aaactgtaga
    10561 caaataaaaa aatttctttg ttctacagtt tttttgttac aattaaagaa aacgttcgca
    10621 tttcgaggag gaatgtataa tgatgtccaa ataatcaatg tatagtcgaa tggaagaaga
    10681 caaaatttcg ctagatgcac ttgttcctaa aaatcattta gtccgtaaaa ttgacaaatt
    10741 attgtcattt gattttattt atctcattgt tgaatcaact tactcgacca ttggtcgccc
    10801 tagcgttgat cctgtcgtct tagtaaaatt agtattcatt caatatttat ttggaatttg
    10861 ttcgatgcgt cagaccatta aataagttga aacaaaccta gcttatcgtt ggtttcttgg
    10921 gcttagttta attgataaag ttcctcattt ttcaactttt ggtaaaaact atgcacggcg
    10981 tttcagagaa accgatttgt ttgaacagat atttatcatt attctcgaat aagcagttga
    11041 tgctagattt attcatgaag aaattttgta tatggattca actcatatta aagcgaatgc
    11101 gaacaaacgt atttttacaa gagatatggc acataaagaa gccattttat accaaaaaca
    11161 attagaagaa gaaatcaatg aggcaagaat tgccgaaggc aaacgtcctt ttggttcaat
    11221 cattcgtcag gaggtggtcg aaagaaaatt tagcatcgca gatccagaaa gtggttatta
    11281 tgtaaaaaca gaacgagaaa aacagtttgc ttattctgct cataccgttg gtgatgaaaa
    11341 tggttttgta ttagatgtca tgattacgcc tggaaatcta catgatagcc gaatgctcgt
    11401 gccacaaatc gaacgcgtta aatcacgtta tggtgtagct gctaatgctg cgtacaaaac
    11461 accttggaac gtcaagtatt taatcgaacg aaaactacga cccattctcc cttatatcag
    11521 tttcgcacaa ctactcgaga tggctataaa aaatatgtat ctaaatcttt tatttgtgaa
    11581 agttgtccat tattgaaaaa ttgtacagaa agccaaaaac accaaaaaga aatacatcga
    11641 catatttggg aagactactt agatgaagca gaacatttac gccatacggt gtacaatcgt
    11701 gaaaaatata aaaaaagaaa agaaacgatt gaacgcattt ttgcagacgc aaaggaaaaa
    11761 catggaatgc gctacaccaa gtatcgaggc ttggaaaaaa tcaggtgcat acaatgcttg
    11821 tttttgtcgc aatgaatctg aaaaaattag ctacttggca gtgggagcat atgtcgttat
    11881 atcttttatt atacaaaaat agacaaaagt atcagtctac actcttagta aagaggaaac
    11941 tgatgctttt gtctacagtc ttagagagtg ggacaatagt tttttcttct ttacgtcttt
    12001 tcctctccat gtattttcct aattttctta atttaacgcc atgagtgcca agccaatgtc
    12061 aatcttggct ccttgtttcc ttcataagtg gaaatccgtt tccgatagat cgaaccttct
    12121 ttttcctcta aaagtttttt tacattctgc tttgaaataa cgccatgtat tattcactaa
    12181 aatttgtcgg tttcgctcac tttttgcttt cgtacattga ctacgaacag gtcagcaccg
    12241 accactttca cattcgtaaa ttttgaagac acgtataaat ccacttttat cttttcttcg
    12301 actaaagcgt tgaccgcaaa agcgacatct gtttcagaaa tatattgttc catatctaat
    12361 ggtaaaattt tttggttgat ggtataattt ttaaacataa agcactcgct tcctttctga
    12421 ttgggtttgg tcactttaat cctacaggga tgagtgcttt ttgtgtacct taaaatcagt
    12481 aaatatccat ccgtgaaaac gggtggcttt ttaacagccc tagaagggct caccactcac
    12541 agccctatct ctctttaact atttaaatgg atctacatac tcttgtttac ttatactgtc
    12601 tctaattcga tcatctgctt cttgttctca aatatatatt tcaattgttt tttgatttaa
    12661 cccaaccgta ctcacgtagt atcctttggt ccaaaagctt ttattcccat acttatattt
    12721 caaatttgca tgtttttcat ggattaaggt tgcacttcgt ccttttaagt agcccataaa
    12781 actagaaata ctaatctttg gcggtattct tactaacata tgaatgtgat caggcattgc
    12841 atgagcttca atgatttcaa catctttcct ctcacacaat tttcttaata tctggccaat
    12901 acttactctt aactttccat aaatcacttt tcgtcgatac ttcggggtaa agactatgtg
    12961 atacttacaa tcccatctcg tatgtgctaa acttttatcg ctaaagctat ttcgtccacg
    13021 cgtagaaacg cgcgtttttt tgtttcgtac gctacgccaa cgaaactgac taaagtcaat
    13081 aaaaaaagtg gggcaataat cagatttttt tctgactatt gtcccactct ctttttatta
    13141 ctcttcatct tctactattc cactaattaa ttagttgaca gatacaatgc tttcacatcc
    13201 tgcacgaaca aactcgggct ctgaaccaat cacttgcgag ttgataaatt caatgaccat
    13261 aggaagatta atccgacata aagtttaatc tgttgtcctt ccattatcag tttagaaatg
    13321 atattagtag gtgtaccgcc taaaagatcg catagtacca caaaatcatt taacgaatct
    13381 atgacagtaa gagcttttgt ctgtatgttt cagtgccatc ttctggtaat aaaggtaacg
    13441 tgtagatatt ctcttgaggt cccataatca tttttgtact ttttttcaat tcctcacaaa
    13501 attttccgtg actaatcaat atcaattttc tactcatttt tggctctccc ttatttcgaa
    13561 atcacacaca atgcagataa agccacacac aagtaatacg ataaaaatcg ctcgagttgg
    13621 gtcatatttt tctttcctaa caaccaataa acacctcgac gatcccagcg agtaagattt
    13681 tagataatat catatcgata tagttttgca tattcaatgt cacatcgcca atcgttggta
    13741 ccataaaaat ttaatatcaa tcattgttgc aactaatgct cctaccatga acactcttaa
    13801 taaagtcgct acgtccgtta atacagttaa gcgagattac actagatgat ctgttcctgc
    13861 caaggcaaga gccagaagcg gttgcctgcc catttgcctt tggtgtgtcg gagctgtccg
    13921 ataaagttca ccgcccgatc cgctctggct ttgtcgtagt gacattccga aagcatgaat
    13981 cggcttggtg tgtattcctt taatttggga tagccctttg gtctggtttc tgccatcagc
    14041 taccacctcc caacagagcc tccatcgcat cttctgctcc gacaccaccg ccatcaccgg
    14101 caatgatacg gcttctggac gaaggcgtca gaccgaactg ctcggaaaat cggttcataa
    14161 ttttcagata ggtctgggca ataaacacct gtggtacctg ctgccaatag ccggataggg
    14221 ttttcacgat gataccgtgc tgggtaatga attcctcggc ttccttccag cgggcgtagg
    14281 cttggcacta accggcaaag gcagccatat ccacatctgt caagattccg atggcctcca
    14341 tctgctttgc cagacgcttc cattccttct ttgcctctgg ttccagccac ttcggacagg
    14401 tcggtgcctt cttttccggc gttgactctt ttgcattgag tgggcgattg cccggatttc
    14461 cctccagctt cttcatggct gtcggggttg attttcttcc tctggttgcc acagtcgtca
    14521 cctcccttca atgtatttct gccagtagac agagaacaga gccaagtcga aacctgactc
    14581 tcttctctat ctgctaactt gtatttcagt ccactcttct gatgaatcgc acccgcagga
    14641 ttttcaagat gccgcaggca aattccaaga agtttctgac cgcatcataa ccatcgtagc
    14701 cgtgtagcca catccttcca cctcgccgcc gtcctcgtcg ctgatataaa tgattctgta
    14761 ttcgctcatc tgttgttttc tccagtcttt tgtttttttg tagtacatat atcactctaa
    14821 aagtgattat tatcaagtcg tttgaaagcc ataatgtaca caatcattcg actggttttt
    14881 ggttcataat tgaccgccaa agtgagcaaa agaaagggac ctctgtctgt ttcagaagtc
    14941 cctttcggta tttggttctt agtggttcag gctgtattct caaacctcat actcatcttc
    15001 gaggtctgcc tctgcaatga tctggccgat gctgtcgtaa cgctggacct gcatctggat
    15061 ggcatccttg tctgctccct cgtccatcat cttcagcagc ttgtctgatt cctttttgta
    15121 ggcccagaaa aggatctcct gaaggctggt ctcctgcttt ttcttgcgaa gggcgacctt
    15181 cttctcttcc accgcctcgt tcaacttggt gaactctgcc caagcaagga taccgtctcg
    15241 cttgattgcg atctccacat ccttcagctc cgggatggtc tttgcaccct tgatctcctt
    15301 caaatatttc tttgcctttt cgctcattgc cattagcgtt tcctccttgt ttttgggatt
    15361 ttcccctttg gttgtgtgta tattgccata gtctctcgac tatatcaagt caatccacag
    15421 gcatatacta cacaatcatt tggagctgta tttgtataca ttatgccgat ttatgagaga
    15481 agaaaaggct ccagccgttc gactggaacc ttgggggggt cggtttctta ggcggcgtgg
    15541 cggtgtagac cttgaggtag ctgatgttct tccagacgtc cgtgcccttc agtctgacca
    15601 tgccgtccag ctctcggtcg agggtgaact tgcggaaaag cttgatgcgg ttgaggctgt
    15661 ttgctagctc cttgccgttc ttggcatcca gaactgcgtc ctcgatctcc gcaatactaa
    15721 tttcctgtct aaaccaaagg ttgctcaagg cttcgggaat gccgtttgcg gtctgcaggg
    15781 ggttgaaagt ttccaataca gcaggggtcg aaaccacctg tccgagctgg aattttgcgt
    15841 tccctaaaat catctgcata atttcttcca tgtgaatcat ccatttctgc cgttgtggta
    15901 ttccctttgg cagtacagta ctatatatca ctctgaatcg tacatttatc aagaagcgga
    15961 tttttgtgga atcaccaatt cgatataata gtaattgcat gaaaaatcta agcactggat
    16021 tggtaggtga tgattatacg tgacgatcac atttacacct gtgattcctg ccactacagc
    16081 ttccctgccg acgaacagcc agagcgttgt cctgactgcg aaaagacagc aactcggctg
    16141 gataccgaaa tcgaaactga agattactat cgtgttcgtg ctgaaatcaa agctgagatt
    16201 aaagctttga atgcgggtta acgacaggta ctgaccggcg gctcaacacc gccggctttt
    16261 tattaccaca tcttgcggaa ttcgctggga cgaacacctt ccaccttgaa aaaagttctg
    16321 gaaaaatagt ttgcatctgt tatgccaatt tttgttgcaa tctcttccat acttagattt
    16381 gtaaagcgta gcaagtgctt ggcctgtgtg attcgtttct gattcagata accgacaaca
    16441 gttgtaccgt aagtttcacg aaaaatctta gctaagtact gtttattgat aaaaaactct
    16501 gcactcaact gatccagcgt aatcttctcc gtgtaatggt catccagata ttgttttatg
    16561 ttctgcaatt ccattttttt tgtagagaaa ttcggcactt tgtcaggatt ccaagaatct
    16621 tccatcaaat gcatcagcaa ttcactgagt agaacattga ttttcatatc ccggacatac
    16681 tcctgtccct ctgccaccat atgaaggctc ctcagcaagt cagcatactt atctgttttt
    16741 gatgttttaa agaccggttg accacctcgt tcaaggtatt ttttgtagac ttccgtcata
    16801 ccaatgccat taaaatggca ccaccgcaaa gtccacaaat catcttctga tgactcatgg
    16861 acatacggct tcaaacagtc aataaacacc atatctccta cttgaagcgt atatttctgc
    16921 cccttatact ccaatgtgcc tgaaccaact tctaccagga aacaaaggta agatgacaat
    16981 cctgtacgcc tagatgtatg tgccttcaga gcagtcagct gtccaatttc ctgcaaatac
    17041 aaaagagagg atcttgcaaa gaacgatgcc gtgtacagca cacgatttga cttcaccgat
    17101 ttcaaagcgt tagaaaacag ttcctccatt acaagatttc tcctttcttt tcttactcga
    17161 acatttccgc cagttcagca gccataatag cttccatagc ttcggcggct tccttgttct
    17221 ctgcgctgac gctgatatag gtcttcagct tcggctcagt accagaagga cgaaccacaa
    17281 cagagcagtt atcttccagc ataaacttca aaacatcaga cttcggcaga ccattcaggc
    17341 ccttactgta gtctaaagtt tcaataacct tcttaccacc aattatatcg agacccttgt
    17401 ggaattcaac cataatttcc tgcatcttgg taaaacctgc cgcaccgtca aactcgaagg
    17461 aatgcagggt gttcaaacaa tagccatact ttgtataaat ctcgttcagc ttatccagca
    17521 ggctgatacc ctgagtcttg tagtaagaga acatttcaca gattagataa gcagcgttaa
    17581 caccgtcctt gtcacggaca tagctaccgg acagatagcc gtaagattcc tcaaagccga
    17641 agatatagct gtcaacctta ccagcctttt ccaacatacc aatctgctca ccgataaact
    17701 taaagccggt cagcacatcg atggtacgaa caccatagtt ggatgcgata cgttcaccca
    17761 aatccatggt gacgatggtc ttgaccatca cagggtcagc aggcattttt ccatgcttaa
    17821 ttctctgaga acagatgtag tccagaagta gcagaccagt ctcattacca gacaacagaa
    17881 catagtcacc atgtgcgctc ttaactgcga taccaacacg gtcgcagtca gggtcagtgg
    17941 ccaacagcag atccgcattg tacttcttgg catactccat acccagagac atagcttcct
    18001 taatttcagg gttcggataa ggacaagttg ggaaattacc gtccggcaga cgctgttctt
    18061 ccacaacggt cacattgctg tatcccatct ctgccagggt gcgggtaaca ggcaccagac
    18121 cggtaccatt cagaggagaa tacacgatag caacattctt gtccacttct tcgccaaaca
    18181 ggacggactg ccccttcact tcttcgatga aagcagtcag aacttccgac tgaatggatg
    18241 caatcttaga agcatcgcca tccatcttta catcagcaaa gatgtccagc ttttcgattt
    18301 ctgccagaat tgcagtagca gcctcggtag taatctgaca gccgtctgca ccgtatacct
    18361 tgtagccgtt atatttagaa gggttatgag atgccgtaat cataacaccg gcatctgcct
    18421 tcagataacg agtcgcaaaa gatactgtag gaacaggaag cagttctggc cacaagtgca
    18481 cctgaatacc atttgcagca aagatgtctg cggcagtctt tgcaaacaca tcagatttga
    18541 tacgggaatc atagccaacc accacggaag cggcttcata gttcttattc agatagtctg
    18601 ccagaccttg gcttgcctta cctacggtgt gtacgttcat gcgattagtg cctgcaccga
    18661 tggtaccacg cagaccacca gtaccgaaag acaaattttt atagaaagag tcctcaatgt
    18721 ccttctcaga ataggaacgc agttcttcta taatatctgc attggcatga ttcagccaac
    18781 gctcatattc tactttataa ttcatattat tatcctctca catccagaaa ctgaatcgac
    18841 ccgtgaatgc tgatttcagt agaatcggcc ggcacaaaaa ggcagtcccc cttatagatg
    18901 tccaacgatt cattatcaaa tcgaacggta ccacagccat tgacacacag caacacacgg
    18961 aagctgattg aatccgcacg atagtgtacc gtctgtcttc tctcagtgtt gacaatcata
    19021 cgagagacct cgaaatgctt gcatctgcaa agcaattccg cagctacacc agcacgatat
    19081 tttagaacac gaagcggctg tttcggttct gacaaccctt tcaagttcgc tacttccagc
    19141 gctttgtcta catgaagttc tctcttttta ccatttttgt caacacggtc ataatcgtag
    19201 agacgataag tcagattgga attttcctga atttctgcaa ttagtgcacc cgcaccaatc
    19261 gcatgaatcg ttcccgcctc aataaagaac acatcattct ttttgaccgg aaccttctga
    19321 agataacgct caacagttcc ctgagcaatc gcttcacgga tcacagcttc ttctgttatt
    19381 ttctgcagcc catagattaa gctggcatcc agtacatacc acatctctgt ctttccaagc
    19441 tgcccgtttt ctttttcaat tgcatattca tctgtgggat gtacctgaac agacaaatcc
    19501 tttttcgcat caatgaattt gatcaggatc ggcaattctc ctttggtctg cggatgcgtt
    19561 cccagaaact ctgggtgttc ttgcaacacc tccgtcaagg tcatcccctg atactctgac
    19621 cagacacaac aaagctcggt ccatccgggt gcgtagagca ttccaagttt ccgcaagagg
    19681 agacatctca attccttttg caaattcatc attcaggcgg ttccccccca cagataatcc
    19741 tttgcggaag gcttcaataa aaacggtttc tgtataacga gccctcctcc gttttttaat
    19801 actctaactc aaatttctgt ttatctttaa cattgatatc cttgccaagc tgtacttcaa
    19861 tcagcttcag ctcagtatct gccataatgg tatgccgaca gccagcactc atggtgatca
    19921 catcgccagc cttaacaacc tgctcatatc catccacgat ggtcttacca gtaccggcca
    19981 gaaccaccca cacctcgtca cggttcttgt ggctgtgata attcatggag tgactagcat
    20041 tcagagtaac cttaatggtc atgctctctg cttctacatc cataacctta taactacccc
    20101 aagacttctc tgcgaacatg atctgctgct cgaacttgtc aacgaaaggc ttaatatagc
    20161 tggactgttc cttatcggaa accagaatac cttcccgaga tgcacagatg accacatcat
    20221 gcagacccat agccaacaca ggaacatcca gttcgttcag gatgtgtacg ccatcgcaat
    20281 tctcattcat aacaccttta ccgataatgg attcctccat accttcggtc agagtattcc
    20341 aagtccccag atccttccac ataccagcaa aacgctgtac ctgaatcttg tcctcatgct
    20401 caacaacggc atagtcgaag gaaatcttag tcagagtatc gtatttgttg aacagatcct
    20461 gataatctac aaagtcaatc agctgatgtg cacgctccat gacatacttc agcttgtaga
    20521 caaacacacc accgttccac agtgcaccct gtgcgatata ttcctttgcc acagcctcag
    20581 aaggtttttc cttgaaggta tttacgaaag cagtctgctc tgcgctctca ggaatgatgt
    20641 agccgtactt ctcggaagga taagtcggtt caatacccat gagcaccaga ttagcttcgc
    20701 ctttgtcagc ctgatcggac agagatttca gggcctcaaa atagtcatct tctacgtaag
    20761 ggtcaaccgg gcaaacaact acggactctt ctggatcaat gcccatcaca tctaccagat
    20821 aagcggtagc caatgcgatt gccgggaagg tatcgcggcg acaaggctct acagatacac
    20881 ccacatcctc accaagttgg ttatggatag cagaaacctg tgtcttagag gttgcaatcg
    20941 taacagttgc gtcgttatct accttcttaa tctggcggta gacacgctga accatggatt
    21001 catatccatc ttcagttttg aaaatcttaa tgaactgctt ggaacggatg tcattagaca
    21061 gcggccaaag gcgcttacca gaaccgccag acagtaaaac aatattcata atgatctctc
    21121 ctttttataa gaaagaggct gctgcaatcc tcaaaaatca cagcaacctc ggtaaattag
    21181 cgttcagcac gcatcgggtt attcaggaag tcctcgtagg acagacggat gccatcttcc
    21241 aactcagtct tatatgtcca tcccagcttg gttgccttgg aaacatccag cagcttacga
    21301 ggtgtaccgt tcggcttgct ggtatcccac agaatctcgc cttcgtaacc aataaccttt
    21361 gctaccatct cggtcagttc cttgatagac agttccttgc cagtacctgc attaacagtc
    21421 tcgtcaccgc tatagttatt catcaggaac acacacaggt ttgccagatc gtcaacatat
    21481 aggaattcgc gcagaggaga accatcaccc cagcaggtaa cagtaggcag acctgcttcc
    21541 ttagcctcat ggaagcgacg aatcaaagcg gggagcacat gactgtgagt aggatgatag
    21601 ttgtcgttag gtccatacag gttagtaggc ataacagaga tatagtcagt accgtactgc
    21661 ttgtttagaa actcacagta cttcaggcca gaaatctttg ccagtgcata agcctcgttg
    21721 gtcttttcca gctcacttgt cagcagacag ctttccggca tcggctgcgg tgccatacgc
    21781 ggatagatac aggaagaacc gaggaactgt aacttcttac agccattctc ccatgcggag
    21841 ttgatgacgt tcatttccag aatcatgttt tcatacatga agtctgccag agcgttctgg
    21901 tttgcgataa taccgccaac ctttgcagca gcgagaaata cataatccgg cttttcctgt
    21961 gcgaaaaatg cttcaacagc gtcctgacgg cacaggtcaa gctccttatg ggtgcgggtg
    22021 ataatattgt tgtagtcctg acggttcagc tcgcgcacga ttgcggaacc agccatgcca
    22081 cgatgaccgg ctacgtagat ttttgcgtct ttttccatca taataatgct tgctcctttc
    22141 taaatttaga gcatctctca ttgcatgaga gaaatcaatt tgtctgcgat ttcgctcgca
    22201 gcatcataaa atgtatcaaa atttgaggtg tctagctgtt tgaggtcctc aatcacccgc
    22261 tctacgcggt ggaggttttc cggtgtgacg gaaattgcca gacccagttt ttgcaattta
    22321 caaatagttg ttctatcttc caaaactgta tcgcgggcga acagtgccat cggacgctgt
    22381 gcgagaatac actccgcaac agttccccat ccagcctttg taataacgta atctgccgcc
    22441 gctatatagt cctgtgtatt gggggtgctg ttagaaataa cggttacgtt ttctcccaca
    22501 agcggaacac ctagcgttgt aatgaaatca tacggcgcgt gactgacatc aatcggctgt
    22561 ttgaaccgcg cggacatacc gagtgcgaca aatacaggcg gtttttcatg ctgtgattta
    22621 atgcgttgca cttcgtcgtt atgaatcgga cgcgccacaa cggaagtcgg tgttttttcg
    22681 ctgttttgga gaaattccaa catttcttcg ttatttagcg cataaagcat ggtgtgctga
    22741 attttaccgt aacgcttggc atattctttc caaatatgtt cgggtaaaaa ttcgcgatac
    22801 agctctgtcc aagtgaagtt tcctacatac aggaaaggta ctcctgccat atcgcaggca
    22861 tccattgccc agattggcat atcgcaaagc actgcgctga cagcttctcg cttgagccat
    22921 tcggcttctg tctttgccaa agaaggaacc tttgccaaaa aatattcaca gatctcggtc
    22981 agcttacccg tgtccaccag tagggtgccc tcacggttaa tcagaccgat atctgtgttt
    23041 attggacgat agcgcacgcg gtctatcagc tctttgggca gttcctgctc caaattgcgc
    23101 tttgcaaaat ccagatgatt tgcgccgcaa accagtgtaa tcttcgcttc gcatcggcag
    23161 agtgttgcca taataatggg cagattccgc gcgatatgtc cgaagccgtg actggaaaga
    23221 tagaaacaga tatgtttatt caatgtcagc ttacttgact gcgttcttca ttgcagcttc
    23281 gcgctttgcc aattcacggt catgctttgc catgatgtta accagttcct cgtaagaggt
    23341 cttctgcgga ttccaaccga gaacggtctt tgccttagtc gggtcgcccc acaggttgtc
    23401 aacgtcagtc ggacggaacc actgtggatt tacacaaacg agcatcttgc cggtttcaac
    23461 atcgtagccc ttttcatcaa tgccagtgcc ttcccaacga atggtcatgc catttgctgc
    23521 gaatgccttc tcggtgaagt cacgaacagt gtgctgctcg ccggtagcga tcacgaagtc
    23581 ctcgggagtc tcatgctgga gaatcatcca catgcactca acatagtcct ttgcatagcc
    23641 ccagtcacgc agggagtcca tgttgcccag ttccagatgg tcctgcaggc cttctgcaat
    23701 gcggccggca gcgagggtga tcttacgggt gacgaaattc tcaccgcgac gttcggattc
    23761 atggttgaac agaataccgt tgacagcgaa catgccataa gcttcacgat attccttagt
    23821 gatccagaag ccatactgct ttgcaacagc ataagggctg taaggatgaa acggagtggt
    23881 ttcacgctga ggaacttctt caaccttgcc atacagctca gaagtagatg cctgataaat
    23941 cttagtcttc ttcgtcaggc ccagaatacg gcaagcttcc agaatacgca taacgcccaa
    24001 agcatcaaca tcgccggagt actcaggaac atcaaaagaa acctgaacat ggctctgtgc
    24061 agcgaggtta tagatttcat caggctgaac aatgctgatg atacgaatca gagaagatga
    24121 gtcggacagg tcaccgtcat gaagggtgat cttgcccatc aaatggtcaa tgcgagcggt
    24181 attggaaata gaagcacggc gagtgatacc gtgaacttcg tagcccttct ctagcaggaa
    24241 atctgccaga taggaaccgt cctgaccagt aataccagta atcagcgctt ttttcatgat
    24301 acattatcct ttcctatgta agtgataagg ccatataaat ggctcgtaat ttctaatact
    24361 tttcaagaat acacgataat tttcgatatt tcatagaaca atctacgcac gcattagcac
    24421 aatattagtt tttcgtgaat tggaatggca acagtttttt tgacaaattt tataaggtgc
    24481 agaacttctt tccgtatgct attccgattg tccttgacaa tgagcctcct cggatgtgac
    24541 gatcttcggc aggagctaac agttaaagtt agactgcctc gctagcgtta gcacatccga
    24601 gctcattatc aaggattcgc tgcgccaatc tctggttacg gtaaccaacc ggtgtctcgt
    24661 agccaagtgt accgtgcaac cgaaggcggt tccaccaatt gacatagtca aataactcca
    24721 aatccaattg ttgtaaggtt tcaaatgtgt attgatagac aaattctact ttcaacgact
    24781 tataagttga ttcagctacg gcattatcaa aaggacagcc tttatgactc aatgatcgat
    24841 tgatgtcaaa agttgttgat aattcatcaa tagcttggtt atcaaactct tttccacgat
    24901 cagtatgaaa aatctcaacc tctgtcagag gttg
//
LOCUS       DASHFF010000037        24577 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.037, whole genome shotgun sequence.
ACCESSION   DASHFF010000037 DASHFF010000000
VERSION     DASHFF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.037"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(152..550)
                     /locus_tag="U2I75_001687"
     CDS             complement(152..550)
                     /locus_tag="U2I75_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN1696816.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="U2I75_001688"
     CDS             complement(547..1320)
                     /locus_tag="U2I75_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1696817.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="U2I75_001689"
     CDS             complement(1317..2000)
                     /locus_tag="U2I75_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1696818.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="U2I75_001690"
     CDS             complement(2167..3054)
                     /locus_tag="U2I75_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1696819.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="U2I75_001691"
     CDS             complement(3051..3920)
                     /locus_tag="U2I75_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1696820.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="U2I75_001692"
     CDS             complement(4057..5343)
                     /locus_tag="U2I75_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1696821.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="U2I75_001693"
     CDS             5616..6194
                     /locus_tag="U2I75_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1696822.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="U2I75_001694"
     CDS             6194..7123
                     /locus_tag="U2I75_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1696823.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="U2I75_001695"
     CDS             7120..8856
                     /locus_tag="U2I75_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1696824.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="U2I75_001696"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="U2I75_001696"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN1696825.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="U2I75_001697"
     CDS             complement(9752..10261)
                     /locus_tag="U2I75_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN1696826.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="U2I75_001698"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="U2I75_001698"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN1696827.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="U2I75_001699"
     CDS             complement(11562..12653)
                     /locus_tag="U2I75_001699"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN1696828.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="U2I75_001700"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="U2I75_001700"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN1696829.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="U2I75_001701"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="U2I75_001701"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN1696830.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="U2I75_001702"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="U2I75_001702"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN1696831.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="U2I75_001703"
     CDS             complement(16616..17590)
                     /locus_tag="U2I75_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN1696832.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="U2I75_001704"
     CDS             complement(17571..19454)
                     /locus_tag="U2I75_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN1696833.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="U2I75_001705"
     CDS             complement(19472..21697)
                     /locus_tag="U2I75_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1696834.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="U2I75_001706"
     CDS             21812..22846
                     /locus_tag="U2I75_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1696835.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="U2I75_001707"
     CDS             complement(22913..23926)
                     /locus_tag="U2I75_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1696836.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="U2I75_001708"
     CDS             complement(24004..24192)
                     /locus_tag="U2I75_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN1696837.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7063 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aactaaa
//
LOCUS       DASHFF010000038        24271 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.038, whole genome shotgun sequence.
ACCESSION   DASHFF010000038 DASHFF010000000
VERSION     DASHFF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.038"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..572
                     /locus_tag="U2I75_001709"
     CDS             <1..572
                     /locus_tag="U2I75_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1696838.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="U2I75_001710"
     CDS             576..1217
                     /locus_tag="U2I75_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696839.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="U2I75_001711"
     CDS             1214..2131
                     /locus_tag="U2I75_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1696840.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="U2I75_001712"
     CDS             2128..2799
                     /locus_tag="U2I75_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1696841.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="U2I75_001713"
     CDS             complement(3340..3828)
                     /locus_tag="U2I75_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN1696842.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="U2I75_001714"
     CDS             complement(4029..4295)
                     /locus_tag="U2I75_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1696843.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="U2I75_001715"
     CDS             complement(4295..4558)
                     /locus_tag="U2I75_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1696844.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5034..5219
                     /locus_tag="U2I75_001716"
     CDS             5034..5219
                     /locus_tag="U2I75_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1696845.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="U2I75_001717"
     CDS             complement(5361..7184)
                     /locus_tag="U2I75_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN1696846.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="U2I75_001718"
     CDS             complement(7782..10487)
                     /locus_tag="U2I75_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN1696847.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="U2I75_001719"
     CDS             complement(10793..11740)
                     /locus_tag="U2I75_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN1696848.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="U2I75_001720"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="U2I75_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1696849.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLHKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="U2I75_001721"
     CDS             complement(12375..12737)
                     /locus_tag="U2I75_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1696850.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="U2I75_001722"
     CDS             complement(12748..13869)
                     /locus_tag="U2I75_001722"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN1696851.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="U2I75_001723"
     CDS             complement(13885..14235)
                     /locus_tag="U2I75_001723"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN1696852.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="U2I75_001724"
     CDS             complement(14365..15876)
                     /locus_tag="U2I75_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1696853.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="U2I75_001725"
     CDS             complement(16198..17550)
                     /locus_tag="U2I75_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN1696854.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="U2I75_001726"
     CDS             complement(17703..18779)
                     /locus_tag="U2I75_001726"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN1696855.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="U2I75_001727"
     CDS             18975..20720
                     /locus_tag="U2I75_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN1696856.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="U2I75_001728"
     CDS             complement(21000..21437)
                     /locus_tag="U2I75_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN1696857.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="U2I75_001729"
     CDS             complement(21421..22794)
                     /locus_tag="U2I75_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696858.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="U2I75_001730"
     CDS             complement(23116..23604)
                     /locus_tag="U2I75_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1696859.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="U2I75_001731"
     CDS             complement(23626..24228)
                     /locus_tag="U2I75_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1696860.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6960 a         5163 c         4487 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcat aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DASHFF010000039        23827 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.039, whole genome shotgun sequence.
ACCESSION   DASHFF010000039 DASHFF010000000
VERSION     DASHFF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.039"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(53..787)
                     /locus_tag="U2I75_001732"
     CDS             complement(53..787)
                     /locus_tag="U2I75_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1696861.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="U2I75_001733"
     CDS             1114..1524
                     /locus_tag="U2I75_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN1696862.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="U2I75_001734"
     CDS             1517..2218
                     /locus_tag="U2I75_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN1696863.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="U2I75_001735"
     CDS             2218..3804
                     /locus_tag="U2I75_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1696864.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="U2I75_001736"
     CDS             3823..4044
                     /locus_tag="U2I75_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN1696865.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="U2I75_001737"
     CDS             4041..4802
                     /locus_tag="U2I75_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN1696866.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="U2I75_001738"
     CDS             5182..5691
                     /locus_tag="U2I75_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1696867.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="U2I75_001739"
     CDS             complement(5766..6305)
                     /locus_tag="U2I75_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN1696868.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="U2I75_001740"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="U2I75_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN1696869.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="U2I75_001741"
     CDS             7318..8169
                     /locus_tag="U2I75_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN1696870.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="U2I75_001742"
     CDS             8197..8832
                     /locus_tag="U2I75_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN1696871.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="U2I75_001743"
     CDS             8852..9625
                     /locus_tag="U2I75_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1696872.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="U2I75_001744"
     CDS             10033..10830
                     /locus_tag="U2I75_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN1696873.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="U2I75_001745"
     CDS             10808..11221
                     /locus_tag="U2I75_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1696874.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="U2I75_001746"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="U2I75_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1696875.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="U2I75_001747"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="U2I75_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN1696876.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="U2I75_001748"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="U2I75_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN1696877.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="U2I75_001749"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="U2I75_001749"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN1696878.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="U2I75_001750"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="U2I75_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN1696879.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="U2I75_001751"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="U2I75_001751"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN1696880.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="U2I75_001752"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="U2I75_001752"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN1696881.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="U2I75_001753"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="U2I75_001753"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN1696882.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="U2I75_001754"
     CDS             21905..22483
                     /locus_tag="U2I75_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1696883.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="U2I75_001755"
     CDS             22535..23017
                     /locus_tag="U2I75_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN1696884.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="U2I75_001756"
     CDS             23134..23508
                     /locus_tag="U2I75_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696885.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atagaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DASHFF010000040        23606 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.040, whole genome shotgun sequence.
ACCESSION   DASHFF010000040 DASHFF010000000
VERSION     DASHFF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.040"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1350
                     /locus_tag="U2I75_001757"
     CDS             <1..1350
                     /locus_tag="U2I75_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1696886.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="U2I75_001758"
     CDS             1484..2827
                     /locus_tag="U2I75_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1696887.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..4390
                     /locus_tag="U2I75_001759"
     CDS             2939..4390
                     /locus_tag="U2I75_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN1696888.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4739..5371
                     /locus_tag="U2I75_001760"
     CDS             4739..5371
                     /locus_tag="U2I75_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEN1696889.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5959..7398
                     /locus_tag="U2I75_001761"
     CDS             5959..7398
                     /locus_tag="U2I75_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1696890.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7693..7911
                     /locus_tag="U2I75_001762"
                     /pseudo
     CDS             7693..7911
                     /locus_tag="U2I75_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8160..8375
                     /locus_tag="U2I75_001763"
     CDS             8160..8375
                     /locus_tag="U2I75_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696891.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8592..8848)
                     /locus_tag="U2I75_001764"
                     /pseudo
     CDS             complement(8592..8848)
                     /locus_tag="U2I75_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9035..9946)
                     /locus_tag="U2I75_001765"
     CDS             complement(9035..9946)
                     /locus_tag="U2I75_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1696892.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9965..10969)
                     /locus_tag="U2I75_001766"
     CDS             complement(9965..10969)
                     /locus_tag="U2I75_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN1696893.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10966..13089)
                     /locus_tag="U2I75_001767"
     CDS             complement(10966..13089)
                     /locus_tag="U2I75_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1696894.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13094..15541)
                     /locus_tag="U2I75_001768"
     CDS             complement(13094..15541)
                     /locus_tag="U2I75_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1696895.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15528..15917)
                     /locus_tag="U2I75_001769"
     CDS             complement(15528..15917)
                     /locus_tag="U2I75_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1696896.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15977..16480)
                     /locus_tag="U2I75_001770"
     CDS             complement(15977..16480)
                     /locus_tag="U2I75_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1696897.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16493..16714)
                     /locus_tag="U2I75_001771"
     CDS             complement(16493..16714)
                     /locus_tag="U2I75_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696898.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16719..16856)
                     /locus_tag="U2I75_001772"
     CDS             complement(16719..16856)
                     /locus_tag="U2I75_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN1696899.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16853..18037)
                     /locus_tag="U2I75_001773"
     CDS             complement(16853..18037)
                     /locus_tag="U2I75_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1696900.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18296..18586)
                     /locus_tag="U2I75_001774"
     CDS             complement(18296..18586)
                     /locus_tag="U2I75_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696901.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18576..19718)
                     /gene="dcm"
                     /locus_tag="U2I75_001775"
                     /pseudo
     CDS             complement(18576..19718)
                     /gene="dcm"
                     /locus_tag="U2I75_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19794..19958)
                     /locus_tag="U2I75_001776"
     CDS             complement(19794..19958)
                     /locus_tag="U2I75_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696902.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19951..20625)
                     /locus_tag="U2I75_001777"
     CDS             complement(19951..20625)
                     /locus_tag="U2I75_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1696903.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20703..20942)
                     /locus_tag="U2I75_001778"
     CDS             complement(20703..20942)
                     /locus_tag="U2I75_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN1696904.1"
                     /translation="MTSKDYLSLFNFRVALGRMSELGYSMMFGEVEKNFFMKHIKGRG
                     YVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(21036..22895)
                     /gene="ltrA"
                     /locus_tag="U2I75_001779"
     CDS             complement(21036..22895)
                     /gene="ltrA"
                     /locus_tag="U2I75_001779"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1696905.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         7285 a         4607 c         4195 g         7519 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     3121 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     3181 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
     3241 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
     3301 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
     3361 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
     3421 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
     3481 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
     3541 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
     3601 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
     3661 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
     3721 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
     3781 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
     3841 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
     3901 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     3961 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     4021 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     4081 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     4141 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     4201 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     4261 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     4321 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     4381 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     4441 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     4501 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     4561 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     4621 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     4681 gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt
     4741 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     4801 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     4861 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     4921 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     4981 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     5041 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     5101 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     5161 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     5221 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     5281 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     5341 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag
     5401 gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga
     5461 aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat
     5521 aataattaac agtgatatat aaacatctat aatatattat tcatactatt gatatttagt
     5581 ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg gaaaacaaat
     5641 aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat ttgcttacgt
     5701 ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt tagaaattgc
     5761 tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa taaagcatct
     5821 ccaacttaca tggttacatg aggatatcaa atcgaaatta ctcaagcttc ctatggctag
     5881 agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct acacgatcat
     5941 tagaaaggaa atcctattat gtatagttat caagaagatg caaaagtcca aaaaaatcgc
     6001 tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga tggcagtatt
     6061 gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa tcaagcggaa
     6121 tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt tggtaaaatt
     6181 ggcgatagcc gtgggaaaat caaagtattt cggattggta cgctgatctt tgtcttggga
     6241 tcgctgttgt gtgggttcaa tcattcgcta ggtattttat tgttcgcacg ggtggtacaa
     6301 gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga agtctttccc
     6361 atacatgaac gtggtcgggc attgggagca ataggtgcgt ttgtttcatt aggctcgatt
     6421 gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta cattttttgg
     6481 atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt accaaaagat
     6541 atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc ggctacgatt
     6601 atcagctttt ttggagcaat ctttttgggg caagaaagtg gctttttcca tccagtcccc
     6661 cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt tgaaaagaaa
     6721 gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac catgagcttg
     6781 attacagcct tgttgatttt ttcttccaac ttctttgtca atgtagtgat tcctttttat
     6841 ctgcaaaatg ctcgcgggtt gagtcctagc tatgctgggc tgttgatgat ggtctttcct
     6901 ttgatgatgg tcgtcggggc gcctttaagc ggctacctga ccgaccgggt tggcccggag
     6961 attttggtat tagtaggctt gggcttattg tcggtaaccc aggtcatgta tatgtttttg
     7021 tcagtggact ctcctttgtg gctatacatt gtcgctactg ccatcatggg tgccggcaat
     7081 gccttgttcc agtcccccaa taatacgatg gttatgagct cggtcactat cgaaaacttg
     7141 ggactggcag gctcgatgaa ttcctttgcc cgaaatttgg ggatggtcag cgggatttcg
     7201 ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccagca agtaactaac
     7261 tatatcgcaa atcggccgga tgtcttttta tatggaatga agattacctt tttaggcagc
     7321 tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa acaaggaggt
     7381 attcagaaaa atgtctgaaa aaaccactgt tgaagaattt ttggaggcct gcgtatgggt
     7441 gagtgggatt tccatggatg atggaagatg aagtgaactt ctaaaagccg aaaaactcaa
     7501 gtattatctt caaaatacta gtgctcaatg agatgcttga tttgatcaca agaagactgc
     7561 gaaagccgct ggcaaccgca agctaggtat cccaccgatg gactacgttt tagaggctac
     7621 tggttatgaa tacggagcca acgctctcat ctgcataaga atacatcacc cagattatgt
     7681 aaagattttc gatacttctg tcaaaaaatc attaagatag ataaatgatt tccgtctagt
     7741 caaattggtt cagaatgtag acacacagat ggtaagaagt cactcacaat tcgagaatgg
     7801 acttgtccta tttgccgtat acatcataat cgaaacgtca acgatagtat caatattttg
     7861 accaagggct atgaatacaa gcattgcctt agaaaacatc aagaacctta ggaacaacgg
     7921 ggatagcttg gttaataaga gataccgctg tttaggcttg caagtatact cttttatcaa
     7981 gaattccgtg gctttagtca tgagaggctt aatactatga aaattttctt tagcattttt
     8041 gatttaggtt ccttttctaa ttataataag atgaccacta cagtaaccta ctgaaatata
     8101 taaacagcca ctgtagtacc attttcaaag tagtctatga gaggagaatc ttcattctta
     8161 tgaacgctgg aattgcggat atgaatttaa taaaaaaaac ccttaatgat tttacttcca
     8221 attcaatatc aaaaggtact ggtatcaatt taagcacaat aaaaaagctg aaaagtggtg
     8281 aaagatcagt ggaaaagctg aatcttttag atgctataaa aataacggaa tttgctatga
     8341 aaaatggaaa agctgagatt gaaatatgga gataattatc gtctagtgaa ttacttgcca
     8401 cttagctgaa tttaacgatc cttggtgctt gaagggggag attcttggga atagagcata
     8461 cttgcaagcc tatgtcttta ctaacaacga tatctcttat taaccaagct atccccgtag
     8521 ttctaaggct tttactttta tctaagcaat aatcgtattc aaaggaaatt tttgaccctt
     8581 agaaagtctc attttatcag acgtgagctt aggcattgct gaattttgtt aattcggttt
     8641 taggttgtgc tttgagcata atatgaaagt attctttatc acgatttcat tcatctaagg
     8701 taatgtgatg tgattctgaa attctttcaa aggcagtttt tgcataagct gacattacat
     8761 catcaatgac atgtttacaa tgtttcacta ctaaaataag ggggtaacca agaagaaaaa
     8821 ctgaatgatt atttgtatct aattccatgg tatatcaacc atttcctata tagactgatt
     8881 atgacatgga ttaaaataaa aaggcactat gtcttaattg tcggttttcg agttaccgaa
     8941 tggcattcat cacccaccta tagaggatgg gcgacttctg cctgaaataa gttacactac
     9001 ttataacttc taataaaatt agattgagtc attattattt aacatcaacg attttccagt
     9061 ttccattttt ttgtaatact aatttgtact gaaagtagct agtggttttt gatacattat
     9121 caaggtattt tatctttgta ctcactagaa aattttttcc gttttgtaga taaactggat
     9181 tctctaactc cgaaaacttt aagtcaatat tgattggtcg catcgcacca ttttcaacat
     9241 aatactccaa ttctttttca gaagctgatg gataaagttt aaaaaatgtt tctaaaaaat
     9301 cggtgatttc ttccgttgtt ttggcatcaa tttctgaatc gttttctact tgttttgctt
     9361 catattttgc cttgtctggc tttccaatca ttgttggcaa actagttact aaatgattcc
     9421 tgttttcatc ttcaaagatt gtcactcgat atgcagaaga aattgattgc gtcttttttc
     9481 cctctgtaat atgttgctcc actaaaaatg aaacgacaaa ttcttccgag tgtttttcaa
     9541 catctagaat ttttacagat tgaacttcgg aactcgtggg aatatctgct cgaaccatat
     9601 cttggattaa agcgagaccg ttttctgtaa ggtactgttc gagattactc attctctttt
     9661 caattgcttc tttgttattt ttccatgaaa aatattcttt cgcaaaatcc tctgtaaaac
     9721 tttcaacgcc agaagtattc actaccttct cttttataac cactttttca tgagttgtat
     9781 gttgatcaat ggcggtgaaa tttttataaa cgccaaaagc aagactacaa gacaataaaa
     9841 gccaaagggc aagcaccatt tttcgatgct tacccacgga tacaactcgt tcttttttct
     9901 tttttgggct cttttccttt tgattccgtt caatttttat cttcattctt tttcctcttt
     9961 tctcttattt gtatcggcca gctccgataa gatgctgccg ccaatagttt tccgttagat
    10021 tcgtccaccc cactggatct cctgcatgaa acatgcgatt ctcccctgcg taaatgccca
    10081 catgggtcac ataagtaccg gcatcatagg ttgaatgaaa gaaaactaaa tcacctggtt
    10141 tggcatcttt tagatcaata tgagtcatgg catcatattg atcctgagcc acccgaggaa
    10201 gtttcaggtc aactttggca taacaccatt gggtcaagcc actacaatca aaccccgttt
    10261 ctggacttga gccaccaaac acataggccg ttccttcata cttaaatgcc tcatcaaaaa
    10321 tttcttggac tattttgtca tcaaactgag ttgtagttag gtattgcttc actaataaac
    10381 agtaaaacat attgccataa ttataccgcc acccaccatt ttccttaatc gcgattgggt
    10441 tggggtaatc gactttttga ccacctgatt tttcctttga aaaagattca gctaaagcaa
    10501 aactatgctt cttcccgttt ttcccgacat gatctagata cccgccacca tagttatagg
    10561 cctgaataat actatcttga tcacacccta gttctttgga acgcttaacc aaagacgcaa
    10621 aatagagaca gccttgtttg atggatgcct cggtacttaa tgaattaggt ggtaatccgg
    10681 ctgactcaga actttgcata acgtcttcca ttgtgccacc agattccact tgaataatcg
    10741 ccaaaagaat tggaatatac tctgttatat ccgattcttt ggcatacttt tccaccattg
    10801 cacggtgttt caatacttct tctgagacac tagttccgcc aggagaagtg ccagaactac
    10861 catttccctc actgtcttca ccaaacaaca aacccacaca catcaaaata cccataaaag
    10921 agatggtcag aaaagttaat gaaataacac ccactttctt caagttcatt ttttctcacc
    10981 tgaccttgat tttggacgat ggttcattct tttttgtact ttttgattgg tctgctttgg
    11041 cagagtggac ctctgtttca cttgttggaa agaatttttc ttacttgctg attggctttt
    11101 cccttttgaa atgatttctt gactagttag ttttcgtttg atttcgggat tcagatttgc
    11161 tctcacaatc cgctttggtg cttctttttt cagcatcata tctttctgag gttgagctgc
    11221 tttatctatt ggaatattcg cccttctttt tccctgacga tttgttgctt cactcagtat
    11281 cttgcgtttt tcgtccattt tcgcatttct tcgttgtcgt tgtttttctc gcaaatccac
    11341 tcgatccgct ttttcttgga acagtccgcg cttgaattct tctggtgctt ttttcgactt
    11401 ttcgattcct ttatggagat tgtatttcaa attcgttggc gtattcctta cctgatcctt
    11461 tacttgttta gtcttatctt tcaccaaagc ttgggtatcc aaaactttgc cgatttttct
    11521 cccagccaaa ttcattcggg attgtttctt attatttgaa actggattct ccttagttaa
    11581 gtcagatagt atttcgtgat cgttcggtaa tcgtgaattc tttcgtagag aagaaccgga
    11641 tggcttaaat ttggattttg attgttcctt tgccaactta tttccgactg ctgcaccacc
    11701 accaaaagcc aaagctcgtc ccacatttcg attgagttgc cgcaattttc gattcattaa
    11761 catttgcggt tttcgcatga cgcgtcttcc cattgactgg ctatcattac tctggaggtt
    11821 gaacatactc ataatgtcgc ccaatttgaa atagataccg gcaaaggtca caatttgcag
    11881 aaaagcaacc atgaaaaatg gataggttgc cgaaatattg aaaaacatcg tggagatgct
    11941 aaatgcagtc gtaatcacca aagtaacacc cgctcgcatc ataatcgtat tgaataaacg
    12001 aataatcgcc ttctttccta aactttcata agtcggcaac atggacaata agaaactaat
    12061 tggcagaaac atcgcaaaga taatgaagag aatttgcgag aacaacataa tccctgttag
    12121 aagaaaaaca aagaaggaaa tgccaatatt gaacaatacc aaaaagacca cggtccctaa
    12181 acggttcatg gtcttggtaa tactgagatt cgcattgtca ttgtcttcaa tctctgcctt
    12241 gaccgcttct tcccgatctt tgcctttgtt ttcatctgga ctcacagata gaatcttgtt
    12301 gacccggtct tcaccaattt cttctatgtt ggaatcatca aattgcagta gtaaccaagg
    12361 ttgttggact tgaatcgaaa acaaactatc ccgaatcaaa tccacactat cttttccttg
    12421 gctttctgaa ttgggtacaa cgatctcggt tccaagggta agggccgctt cactaacatc
    12481 tgagctaaag tcattgattt ttgtgatata ggtaggcgca taagcaatga acgaacccga
    12541 tagtagaaaa atcatcacaa aattaatcac ggctcgaact gctttcgtgg tttctcgttt
    12601 taataatccg gtatacgcca catacacccc cagagctaaa atcatcaata atagaaaccc
    12661 aaaataaaat ccggaggtct ggagtccatt ttcagtaata ccagccaatg tttggatatt
    12721 ctttccaata ctttcagccg tatccgaaat gaaatctaat ttgtaagcct cttgaaccac
    12781 ataacccgtg gcgttcgaca aatacaaact caccgtccaa atgaaattag taatggcata
    12841 aagaccatac atcacgcttt tccctaagcc atcaccccaa ttccaaggca accaatccca
    12901 agagctatcc acaaagaaat ctagttgata gttgttcagt gaatatttgg aatatagatt
    12961 tcctgcttca actgtatcat caactagccc tgtcgcctcg gctaccgtac caactaaact
    13021 caatagaact atcagagtga gtatgctaat aaaaacgatt cccacaatcc gaaaaagttt
    13081 ctttttcatc ccctcactcc attctctctt gaacaggtgg acgggtatca aaggcatgga
    13141 agagttcttc aaagactgga tgtacctgaa ccacgcctac ccgaccatac aaatcctgca
    13201 tcaagcattg gccattttcc aaatctcgta ggcgtttttg attgctttca tcttctcgat
    13261 ccaagccaaa gaattccaag gttttcttga tttcatgaat atcggtactg cgaaacgcaa
    13321 acttcaaccc gatattattc ttcaagcgtt catccagtaa atcatcacta ttttgcgtga
    13381 caaagtaaac cccggcattc atagcccgcc cagcacgaac gagcttattc gataatgttt
    13441 tcccttgcgc tacttgaaga aaactccacg cctcatctaa gtctacaatt ttgaagattt
    13501 ctcgattact atggataaag tccaaggcaa aagtggaaat gacgattagc atggaaacac
    13561 taagcaattc catggtggta tattcttcaa aagtggtttc tgcatctggt aaaaccaaat
    13621 ccgctacttg aatgatattt aactgcctat ctaaactaat cgactgggta atcgaaccat
    13681 ctgagaaaag caaatgggca aaatcataat cggtaaagga ctcgatgtga tccgcgatat
    13741 tttccgcaac tggtgagcct tcttttcgaa gctcattaat tacttgtaat aatccccgtt
    13801 gattgctttg ggtaaccgtc cgaatggcct tacgcaacac tggaaacttc tccccatctc
    13861 gactagaaat tcctgttaaa aaggtcagaa tatcaattgc tagactttca gaatccttct
    13921 ttttctccat aatcacataa ggatcaagta atccttgatt ttttggctta ctagttaagt
    13981 tgagaatatt gatttcatcc gcaatttctg gcaaggtttc tttccagcca ccccgttccc
    14041 cttttggatc gacaatcaca gcttgtccac caaaaaggac ggcgtaataa actaagagat
    14101 tgttactaaa ggactttccg ccaccgagtg aaccgaggaa tgccgctgct agcgcattgg
    14161 taactgaacc cttgacccct tgtgaggcca atgccggttt caggtaaaca ttgcgccccg
    14221 tatctacgtt ataaccaaag taaatgcctt ccaactcccc taacatctga gtggcgccaa
    14281 aaccaagtcc ggcgagaaaa tcagacgtaa catactggat ataatcattc atgtatcgct
    14341 tactagaagg taagaattct tcatgtagcc ccatcatatc gccaaaaggc cggactagtt
    14401 ttacattggt atcatcataa aaatctagca cttcatcaca ccggcgcttt agttcatcta
    14461 ctgattcggc acttactcga accacatagc tcagtttgta catggattct ttggactgat
    14521 ccaaggtagt ttccaattca tctacggaat ccaatgcatc taagacattg gaatttgttt
    14581 cattgtcaga ttgataggca tggttgtcca agtcttttaa ttcctttttc ttattgcgaa
    14641 cggtagcaag tgcctttttg ttcgtcacaa tctccacatt catactagtg tcaattggga
    14701 atgtaaattg ttgttgctgg taatagaaaa gttctgagga gggaaactcc atttccccca
    14761 caattgtatt aattgttaag tacgctacat atgattcatg attctcatgt tccatgcata
    14821 agtaccgggg cttttcttca atcaaacaac gactgggacg taataagtcg taacgtttca
    14881 ccaaagtttc cgacttcagc tttttctttg gtagctgaaa ttcatattct tcaaatggtg
    14941 tacccttttc gccatagata tgttcaatca aatacatcag atcactcggt gtgactcgtc
    15001 tgactttaaa tcttcgagcc aatttacttt ccattagttt ttcaagcttg gtatatcggc
    15061 gaatttcttc attggataag gaaacaaaat cgcccatcaa atgatgattc actccataga
    15121 caaattcttg aaagccggag aagaaagatt tcttgagatt ctttagattg acctcttcat
    15181 ctgtcgcaat cagtttgaag ccaataaaaa agcgataatc aatctgacta tcgccaatca
    15241 tggatactaa tgcctctgtc tgtaaatcaa ttcgttgttt cgctacttct gttaatcgcc
    15301 cggtaatttc cttttttgat cgttcttgcg tggcacgaat actactttct gtggcaattt
    15361 gtaaggcatg aatcttgcct tcacgatttt gcgcaatcaa ttgacgaaag ttatcgtgga
    15421 cttgatattt ctgttcaggt gacaaaaagg agtaattgta aggtattaac tcatagtagg
    15481 caaaacattc tccctcttga ttaaagacaa gattattttc aatatactta atcgggtaca
    15541 tagttcacac tccttaccag agtaatgggt tcgtcccatc gtttcttctg ttctttgact
    15601 ttctttcctg cgaaagtgac cttaaatcgt aagtgatagc taataaaaga tcgtaaaaat
    15661 cccaatggtt tcttaccgtc aaacgttttt tgcgacatga accaagtaac tgccaccggt
    15721 atacctaagt attttaaaaa ggccccctct atcatagata gtggaggcac atgggcaaat
    15781 acgatgacaa acagcaagga taaaacaaac caagccatct ggttaaaagt cacaggaaaa
    15841 ggcagttgaa agtcattaat tgcataaatg accttttcaa ctgcccaaat actggtgtag
    15901 ctttttattt tcttcatcaa atcattcctt taaaaagaag gatagtttag aaatcaacta
    15961 cccttcaata attttgttaa tatggatact caaaaacacc tcgactcgtt accaaataat
    16021 tgcctccaat ctccaagtct ctaccacacg catcaaaatc aatatacgtc ttcaagtgtt
    16081 ctggtacttc tccaaaatca ccagcttcta caatcaagaa atatgctaca tcactcatat
    16141 catcgcaatc tggataatag ataatatcat ccacatgctc taccatctcc tcaaaagaat
    16201 tgaagaaagc atgttgaatc tcagaagcaa cttcaccaac tggcgttcct tcaagttcct
    16261 ccgctaagcc acacaaccga ttaatttcct caattggcgt atactcatca atctcaaagg
    16321 gaagttcata atcatgaatt gcatattcct cgtattcgtc atttaaccca attcgttctt
    16381 tcacttcatc aaaatcgaca ggtggggtaa accacgcccc caccaattca ccttcgttat
    16441 atttcccgag gttcgcaata taaacacgca tctgttccat cgtgcttccc ccttacgcac
    16501 cgacaattcg gttaaacaaa ctgagtaaga catccttcac cccagaagca ttaaagacca
    16561 atccgaccgc aatcaaagca atcaccaaaa agccaatcag tttggaaaac tcccgcttaa
    16621 atcctaaaaa caaaccaatg accacaattg ccagtaagac taaggattgg gcattggtta
    16681 aaaaccattg atataaattt tgtccgaaat tcatcttgtc actttccttt cttttccatt
    16741 gctcgatccg ctgctgcact tgcattcatc aagcacatca cagtaacacc aagcatactg
    16801 ccaccaaata ccaaaaaaat atcttttaca attcccatga cgattcctct tttcatataa
    16861 tcaaattttc aattgccata ctttgttgct ccaaaatctt tttatgtcgg tctgataagc
    16921 gtgcattctg taccatatcc tggatatagg ttgtccgatt caatcgatcc aactccttag
    16981 ccattttcca cgtaggggcg acttgtcggc ctagccaatt caaggttcgt tcaatggtat
    17041 acggttttgg ctcggaagtt aattgaatct ctttccgatt ccgtccaata aaacgccccc
    17101 attgttcatt caagggccag ttggttcgcc ttttaccttc tactttatcc gcaaatcgca
    17161 agtaacgatt gataatggaa aacgtggtgc tctcaatgct acgacctttt aacaaatctt
    17221 gaatcgcatg aaaagcacgg tcatttttca gtcgaatctc aaaacgattt ttcgtctcag
    17281 tttggtctaa tggaatccct aacttgatat attgttcata gtctttttca tacaaacaga
    17341 aataaacctc actcttgaga ctgccgatat ataaggtatt gcccatgccg tccttttcat
    17401 cagcctttaa aagttccccg gaacgataac ttttaaaggt acgaaacaag gagatacatt
    17461 cttccttctg acattttttc gttagctcag gtatatccaa tagccccact cgatcattga
    17521 ttgccaagtc caagcgtttc atcacgccac cggcctttag acaatcctcg aaaaagtcgt
    17581 accagctacg cttttgagcg agtaaaaaag tttcaaattg tcggcaacct cgccccttta
    17641 attctaaaag cacaccttta tcttcttcat gagacaacat caccacaata tcacccatga
    17701 catactgttc ttgataggag taaaaggcat aatcttcatg gagcatatac tcaaatttca
    17761 aatggagaat ttcttgaata atttttcgtg catccgttgt gggaaagcga atccttacat
    17821 agtcaaacag taacgtcaaa ggcaaatcgg gattaaagct ttccagttgg tcaaaaattt
    17881 tcctcatggt ttttatagat gcggttacgc caccattttc tatctgattt aagtacggcc
    17941 gactaagtcc acaagccgta gccaatttat tttgtgaaac gccgtattcg ttgcgtcggt
    18001 cttttaatag ccgataatct aaattccgtt gtgccaagtt ctcacctcca aattcaaggt
    18061 gttaactttt tcaaatgagt taacacctat acaaattact tactatcttt gatacaacaa
    18121 gcattcttaa tggttgctta cttatttttt gtgtttttgt acccccctgt tagatacggg
    18181 ggtgagacat catggtcggc taacgccgtc catgaacagg cacagcacca cggaggattt
    18241 ctgcttcgcg aatcctccgc ggtcagcgcc taacagtcag ttgtgttggt ttaggttact
    18301 catctgtaat accatttact tccaactctg ctttcatttc taacaagtat tgacgaatat
    18361 ctggttcttt tgcttggtcc gcaatccgct caaattctgc aattacttga tttatttcaa
    18421 ttcgagcatc cgtctcctca ttcattaaat atggatcgcc agcgactttg acaccattct
    18481 tgcgaatatc aaattttaac cgtcgcaatt gttcttctaa ttctgggtct gactgaatct
    18541 ttccaatcac ccaataaatt tctccaatca actcatcata aaccatgtac atccaactct
    18601 tttaatcttc tggcaatatc aaaaatgacc ggaactgtta cagagtttcc ggcttgctta
    18661 tacaactgac tatctgaatt gacttgagaa tctcgatcaa aagcccaatc gggaaagccc
    18721 tgaagtcgcc aacactctct aggtgttagc ttccgaattt gatactgatc cgtgacgatc
    18781 gcttgttggt ctcctgttag taaggtatga acactctgtt ttcccagacg acctcggcga
    18841 cttgtggaat taggaaaagc cagattcaca ctgtcttctg gcttcacctc ttgatattct
    18901 gtttcatttt ttcgaatcat gatttttgta ccaccttgtt tcgttgacaa ggtgggggcg
    18961 gttccagtaa cttcataaac tcgattacct tgttcaaact ttcccggtaa tagtcctgct
    19021 acagagattc catcccttgc tggcagtgcg attttgattc cttcatcacc tgttaaggtt
    19081 ggcgctacgc catctgaacc atagacttgt ccacccattc ctcgattgga aggattgata
    19141 tttaccaaat ttctgatgtt ttgatcagat gttgaagacg gtccaatgat aggaaatact
    19201 tgttcggtac atgcgtctcg agaatgtccg ataaggaata tccgctcccg gctttgggga
    19261 atgtaggcct ttgagttaag cacctaccac tccacgtcat accccaatcc atccagcgtt
    19321 cggaggatcg tctcgaacgt agcccctcct tcgtgattga gcaatcctcg gacgttctca
    19381 aggaataaaa gcttaggtcg gagaagagtg gtgaaccgag cgatttcaaa gaataaagta
    19441 cctcgagtat cggaaaatct ttgccgcttt cccgcaattg aaaaagcttg gcacggaaat
    19501 ccgccgcaaa ggatgtccac tggtccgagg gattgaataa actcatctga tacacttgtg
    19561 atgtcatgca tttccacctc cttacttgta tcatgaattg ctttataact acgtctagca
    19621 aatttatcaa tttcacaaaa accgatacaa tgatggctcg cctgttccat acctaaacga
    19681 aagccaccaa taccggcgaa taaatctaaa aatctcatac attacctcat tcattgcaaa
    19741 gaaaaaagcc aagaacattt tgctcttggc tcatacatta ttaatttgtc ctactatttt
    19801 ttatttaatc caactaattt ccgttgtttt tcattggcct gatcggctac atatttaatc
    19861 accgttttga actcttttgc agtagcattt tccgataaat ccgcaatgct cttattgtat
    19921 ttctttacaa ttttcgttac ttgttctttc tcagtcatgt aattgaacct ccaaatcttt
    19981 cctactatac cctagtggta aaaataattc tagattatcc gtagcataat gaacttcgga
    20041 aatattattc gtataatctt ttattatgat atgaagcaat acatctggat gtttactctt
    20101 tacttttgca atgttctctc ttgatttgaa aaaagcgtta ataaatgcct ttttcggcac
    20161 atgacgtccc tctgcaattt cacgttcttg tgtaaatttc caagcgattt tagggtcttg
    20221 ataaacatag aaaatcgtca ctcgaaaatg ttttttcaac gcccgcgaga tatttttttc
    20281 agcacttaat attgcaaaag ttgcatccag aataaaagaa tagccatgat ctaagacata
    20341 tctaaatgcc tgctctacca accaagaaga tgctttttga aagagactag agttttttcc
    20401 attataatca ggaaactggg tgcgaaagta atccgcatca ataatcacat ggttatcata
    20461 attctcagct aatccaatgg caacctctga ttttccagcc ccaggactcc ctgccataaa
    20521 aactgcgtct cgtccaactt cgggggattt ccctttgata atataagaaa taaacttatc
    20581 cttatttgct tttgcatact ctaaataaga atcattactt tccatattaa ttatcccctt
    20641 tcttcctact atataaaagt attatacgtt acttgaagta taaagtcagt attgaagatc
    20701 actcaatcat tcatcttttc taatttacta attgaatcta agaaatcata cccttttggt
    20761 acaagtggcg tataaaactc actaatcaca ctgccacctg tatccacata acctcgacct
    20821 ttgatgtgct tcataaagaa gtttttttcg acctccccaa acatcatgga atatccgagt
    20881 tcactcatac gacccaaagc cacgcgaaag ttgaatagcg ataggtaatc ctttgatgtc
    20941 atctccagat tttcccccgc ttcacaccgt acatgaaact ttcacttcat acggcgttcc
    21001 atcaaaagaa tgttaagtat gatatatttt tcaagttaaa ctgttaaaac actattacca
    21061 gctttgacac gtaattcatt tagtttttct atttgttcat tggttagatt taatacattc
    21121 aggtatttgt gtatgatttt ttcttcgata gcatggacta atgcgtgaac atttttgtga
    21181 ataataatga ggttgctgta tttgtctgta ccctgttgtt ttactggtac gatatggtgg
    21241 caatggattt cttcgtattc gagaaattta cccgtgatag cacattttcc cttttgagca
    21301 gaatatttag atattctgtt atccacaaat tcaatacttc tttcataatt actatttctc
    21361 atcatgtgaa gtaaaagggc taggtctaat tttaatttac tgtgtatctt gcttcgtcca
    21421 ctttcagtgt acttgcaagt tccctctgat agggggacag caagtctaaa ctgaacatag
    21481 ttgagcggta caattggcac attattcaac catcgcattt ctttactttt tccatacttc
    21541 gatttgataa atgtatttaa tatctttcca tgtttctttg tcggcagttt gtgattgatg
    21601 aacggtttta gtgtataggc tattttactg caatcgagat ttacacaggt tgcaattcca
    21661 taatattgat ggattccaat caccatagaa ttatataatc caatttcttt tgcttgttcg
    21721 caatgattct tcggtctggc aactttacgt aattgtttct ttaattggat tttaactctt
    21781 gtcattgatt ttttgctcat atgactttcg actacgtatt ttttaccttt cttgattagc
    21841 tttaattcaa atcctagata ttctgattta ccttttttga gattgacaat tttggatttt
    21901 tcttcgctaa tttcaagtga taaccgttct ttaagccaca ttttcgttgc gtgaaatatt
    21961 ttatctgcat gacttcgttt tctacaaaag attttgaagt cgtcagcata ccgaacaatg
    22021 tatatttctt ttaagtttgt tcgtcttaat gcccgatact gattagtctt atcaatgcct
    22081 ttgcctttat ctcgcctatc cataatcttt tctgtctcaa tcgtggtatt ttgccattgt
    22141 gaagcaaccc accaatctaa ttcattcaat atgatattag atagtaaagg ggataggaca
    22201 ccaccttgag gtacaccttt tttagggtgt tcgactttac cggttggtag gactatagga
    22261 gcatttagca tggatcgaat aatacctaat agccatttat cacggattcc aatcgtccac
    22321 atttgacgca aaagtttaga gtgattgaca ttatcaaaga atcctttaat atcaacatct
    22381 actacaaagt gtagcttccc cctatttgcc agtcgattgc aggtagcaat cgcatgatgt
    22441 gtggaacggt ttggtcgaaa tccataacta taattgtgaa atttagcttc gcatatcggt
    22501 tctaagactt gtaaaataca ctgttgtatt aacctatccc agattgatgg aatgcccaga
    22561 gggcgtgttt ttccatttgg tttgggaatt tctacacgtc taactgctcg tggtttgtaa
    22621 cagcgaaaaa gaactttaac ctcagttaga aaggtatctt ctgacagtgt tttaatgtct
    22681 cgaatacttt tcttatcggt actcgctgta taactgccct tgttattctt gatatttctg
    22741 tatgctagtc gaatattttc atcactcata ataattgtca tgagctctgt aaaaacttct
    22801 tttttatcac tttttacata cagttcatca aatatttgtg tcatgccata gtattcacta
    22861 tgacgaagtg ttttatttgt ttttacttta ttcatagcac cactaccttt gtttacttag
    22921 gttgtagtgt ttattttgtc ttactcgatt ctatgaaata catcatttaa ttaacgattt
    22981 cttttgacta gtggctattc ctccgatata tttctatatc ctcattggta ctgtgccacc
    23041 actttcactg gcataaatgt gcttattatt cttcgtcaca aatgtttcaa tggaatatag
    23101 tttcctccac attgccagct tcccatgttt cgatattttt atctttatat atacttttag
    23161 gtgcttgctt tgaacctgta tatttccaat gcctgtaaca ttgcatggtt gttcataacc
    23221 aataatctta ctaaaccaca acatacattc tgttccgttg atacggtaga atcctgtaca
    23281 tttctataca gtccgtgtat agattctttc attcgcaagt tcgtcagtag tattacttac
    23341 attctcaccg tgagtatttt atagacctcc gacctatagg ttacatcatt cacctctgaa
    23401 caactttcgt atatgaattt atcatattta cggtaacttt cagccgactt caccgagctt
    23461 ctgaccatct aacagctata tgttaaacag ccagtcggag tattaaggga gcgtttcagg
    23521 gcgttacccc atcattccac tgctcaaaat atcagttctc cttaaaagga aacttatgtt
    23581 ttcttctttt aagtgcctaa cctttt
//
LOCUS       DASHFF010000041        23460 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.041, whole genome shotgun sequence.
ACCESSION   DASHFF010000041 DASHFF010000000
VERSION     DASHFF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.041"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(74..655)
                     /locus_tag="U2I75_001780"
     CDS             complement(74..655)
                     /locus_tag="U2I75_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN1696906.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(661..1920)
                     /locus_tag="U2I75_001781"
     CDS             complement(661..1920)
                     /locus_tag="U2I75_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1696907.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1922..2968)
                     /locus_tag="U2I75_001782"
     CDS             complement(1922..2968)
                     /locus_tag="U2I75_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696908.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3203..3478)
                     /locus_tag="U2I75_001783"
     CDS             complement(3203..3478)
                     /locus_tag="U2I75_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN1696909.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3721..5031)
                     /gene="der"
                     /locus_tag="U2I75_001784"
     CDS             complement(3721..5031)
                     /gene="der"
                     /locus_tag="U2I75_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN1696910.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5213..6442)
                     /gene="rpsA"
                     /locus_tag="U2I75_001785"
     CDS             complement(5213..6442)
                     /gene="rpsA"
                     /locus_tag="U2I75_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN1696911.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGGILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6577..7257)
                     /locus_tag="U2I75_001786"
     CDS             complement(6577..7257)
                     /locus_tag="U2I75_001786"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN1696912.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7306..7941)
                     /locus_tag="U2I75_001787"
     CDS             complement(7306..7941)
                     /locus_tag="U2I75_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1696913.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(8007..9410)
                     /locus_tag="U2I75_001788"
     CDS             complement(8007..9410)
                     /locus_tag="U2I75_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN1696914.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(9397..10428)
                     /locus_tag="U2I75_001789"
     CDS             complement(9397..10428)
                     /locus_tag="U2I75_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1696915.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10459..10680
                     /locus_tag="U2I75_001790"
     CDS             10459..10680
                     /locus_tag="U2I75_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN1696916.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10845..11582)
                     /locus_tag="U2I75_001791"
     CDS             complement(10845..11582)
                     /locus_tag="U2I75_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN1696917.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11735..13075)
                     /locus_tag="U2I75_001792"
     CDS             complement(11735..13075)
                     /locus_tag="U2I75_001792"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN1696918.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13312..14835)
                     /locus_tag="U2I75_001793"
     CDS             complement(13312..14835)
                     /locus_tag="U2I75_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN1696919.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(15213..15824)
                     /locus_tag="U2I75_001794"
     CDS             complement(15213..15824)
                     /locus_tag="U2I75_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1696920.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(15947..16072)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(16154..16870)
                     /locus_tag="U2I75_001795"
     CDS             complement(16154..16870)
                     /locus_tag="U2I75_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1696921.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(16876..17445)
                     /gene="scpB"
                     /locus_tag="U2I75_001796"
     CDS             complement(16876..17445)
                     /gene="scpB"
                     /locus_tag="U2I75_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN1696922.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(17429..18226)
                     /locus_tag="U2I75_001797"
     CDS             complement(17429..18226)
                     /locus_tag="U2I75_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN1696923.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(18196..18567)
                     /locus_tag="U2I75_001798"
     CDS             complement(18196..18567)
                     /locus_tag="U2I75_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1696924.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(18586..19473)
                     /gene="xerD"
                     /locus_tag="U2I75_001799"
     CDS             complement(18586..19473)
                     /gene="xerD"
                     /locus_tag="U2I75_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN1696925.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(19569..20041)
                     /locus_tag="U2I75_001800"
                     /pseudo
     CDS             complement(19569..20041)
                     /locus_tag="U2I75_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
     gene            complement(20146..21009)
                     /locus_tag="U2I75_001801"
     CDS             complement(20146..21009)
                     /locus_tag="U2I75_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN1696926.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(21205..23304)
                     /locus_tag="U2I75_001802"
     CDS             complement(21205..23304)
                     /locus_tag="U2I75_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1696927.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT         6419 a         4993 c         3938 g         8110 t
ORIGIN      
        1 acaaagatag gctatgagga aagattccac atccagctga cctttgtctt ttttagtact
       61 atactatttt taactaattg ttatgttcac ttacccggtt acttttcttt tgtgtctgca
      121 aaaggatctt tcggttaact tcatcagata atttcaaaaa agcatcccta tctccatctt
      181 ctagtgcttg atcgatttga gcataaagca tttttagttt tgcctcttct tctttttctt
      241 gaaaatatcg ttcaattaat tcagtatctt cttcactgac tgaagaatcc caagaagaaa
      301 atggattgtc ttctaagata gatagatatt ctcttgtctg ccaagcattt tcgaatatac
      361 attccacata taaaggcttt ttccaattca aacggatttc atgaaaaatc tggtctgtgt
      421 ccgtaaattc ttttccctgc aaaaacaatt tcatgggttc cccttcaaca gatagatcac
      481 tgatctgcag tcctcgatcc gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa
      541 tcgcttcatg attggcaaga taattcaaga tccaaaaaac ttctcttcgg ctaaaagata
      601 catgattgac catccacgtt aaaaaattct tcttttcccg tacatcaatt agcatttttc
      661 tcacctctct gttaggtcat ctaataatga cagtgcctcc atatctcctg gttcaagtgt
      721 taaataataa gtcaataatt cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa
      781 ggcatactct ttcaagaaat ctggttcatg tgatagttca tgataagcct gttcatagtg
      841 tacagctgca acggtaaagt cttctaattc attataagca tgggctaggt tccattctgc
      901 ataaggatta gcagtttctt ccatctgatt gatcacttgt atcacatctt catatctttc
      961 atcatccaag taaagattac ttaaagtaag taatgtttca tcttgtttgt ctcctacttc
     1021 taaagctttc agtaaaagtt cttctgctcg ctgtctatca tgcagacgga aagcattttc
     1081 cgaggccagg tgataaagtt cgacttggta aggattttct ttgattcctt cttctaacac
     1141 tgtttgtgct tcttcaatca tttcttcttc ttgaagagct tgtcctagat atagatataa
     1201 cgattgataa tgcggattaa cttctcgaag ttcttccaga tagttgattg cctgctgatt
     1261 ttctttcaat tgaagattcg taaaagctag ctggaacagt cgatcatctg tcctttcttc
     1321 tttcagtgct tcttctaaag gtacgatagc tgcttcgaat tctccttgca tacttaagga
     1381 ttgtcctaaa cgttcctgca tcgagattcc tgaaatttca tttatcccgc ttgccaacaa
     1441 tgtttcataa accactgctg cctctttgaa acgatcaact gaaaaataca attcagctag
     1501 cgcaaactgg atcaatggtt catcaggaag caacccagct gcttctttca attttgcttc
     1561 actcacttca gggattccga taacttggta caaatcagct atcgccaata agctttgcgg
     1621 atagtaatca ctttgcttat ccactttttc caggtattca aaagctaagt cgatctcatt
     1681 atcttcgatt gcaatttctg ctaacgggat gttcagtccg tcattttctg ggtattggtt
     1741 gatcagttgt tcaaaaatct gttttgcttc gatcaaaaag ccaatagcca gcaattcttc
     1801 gcctagttca gctaatatat ctgctgggtc ttcttttaat gctttttcta aaaagatttg
     1861 tgcttgtgac aagtcttcat ctgataaagc ttgcaacatt ttttcgctat ttgtattcat
     1921 ttcaattccc tcttatctca ctgacgattt gttgaaatac cgtcattgtt ctcaataatt
     1981 ctttttcgtt tggatgttgg tatccgtcta ttatacctat tttttctaaa acaagtattt
     2041 ttttgctttt ttctgccaaa aaaagaactt tttccaaata ctccatcttt gaaatctgtt
     2101 ccagccagtg gattggatag ccaagcaagt ccagccacac aaaaaagttt tttagttgga
     2161 aatcaaaagg atatgcttgg agattccaca cgaaatgaaa catcattcct aaaaattttt
     2221 tcatactagc agaaagtaga tggccatttt cggtcagata aaaagcttgt tcaaacagtt
     2281 taccataatt tgacaggttt tctgcctcta attgataaaa atagatcatt tcttcgatca
     2341 tcccggcaaa cggccgagtc gttaattgct ttttatttgg atagttttta tacagatttt
     2401 gcaaaaaacg atgatcacaa atcattccgc aacagataaa agtcaaaaag tcaatctgct
     2461 ttccgtccac ctgccgctcg ctgattgttt gatcataaac gatgcgttct ggcagattga
     2521 tggtttgcag cactacacga tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc
     2581 ggcttagaga acggatcgat acagggatca tccatagtga tgaatcaagg gttgtatgtt
     2641 tttgaaaaaa acctgccaga tccatgatcc cttcatttcc aaatccgatg ataaacatgg
     2701 attgattttc gggatagcgt ttgacgaaat caagcaggtt ctttaattct gttaaatgat
     2761 tgcattgagt gttggcacaa atataccagt cattgtttgc ttgatttgca aataacgggt
     2821 tgatcttatc tgcaaacaaa tcataatagc gctgattcgt taaaaataag attggctgat
     2881 caatggaaag ttctcttagc tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg
     2941 catcttctac ttgataatca agttccatac gcatccctcc tcttcttatt tttattttac
     3001 cattagtaag caaattctag aaaagataag agcaaagctc acctcataac ttgcaaataa
     3061 ataaaaatta aataaaatac atgtgttttc tatgtatgac atacggattc ggaatctctg
     3121 tctcttattt attccttttc tgctaggaaa taaaaagaaa agcacttgta gccctagagc
     3181 cacaagcgct ttttcaactg tcttatttaa cagcgtcttt taatgcttta cctggtttga
     3241 atgcaggtac tttgctagct ggaatttcga tttcttcacc agtttgtggg ttacgacctt
     3301 tgcgagctgc gcgttcgcga acttcaaagt taccaaaacc gattaattga actttttcgc
     3361 cgttagctaa agcatcttga atagttgaaa atacagcatc tactgctgca gttgcatctt
     3421 ttttagttaa atcagtagct gaagcaactt tttcgattaa ttctgctttg tttgccatgg
     3481 atagattcac ctcctccaca agagatatga tagaaatatc atttagttaa aactttctga
     3541 gtggtccaga agtttaaaaa gaaatattga ttaacttcaa tattctgtta tcacgatatc
     3601 atatgaaccg cttaatagca aggtttttac caattttcac atgctttttt tagtcataga
     3661 aacgtaacaa acgaaactgt ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc
     3721 ctattttctc ctacgaggga taattcggat ggacgtccct tcaaaagtaa atgcttttct
     3781 gatttgattt tctaagaaac gttcataaga aaaatgcatc aattcctctt cattgacaaa
     3841 aacgacaaat gtaggtggtt tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt
     3901 tcctttatct gttggtgttg gattgattgc tacagcatcc ataatgatat cattcaacac
     3961 agccgaagag atacgaagat tctggttcat acttacttgt tcgatcattt caggcagacg
     4021 ttccaaccgt tgtttagtca ctgcagaaac aaaaacgatt ggagcgtaat caagataccg
     4081 gaattcatca cgaatctctt gttcgaaatc acgcattgta ttggtttctt tctttacaag
     4141 atcccattta ttcacgacga taataattcc acgaccagct tcatgggcgt aacccgctac
     4201 tcgcttgtct tgttcccgaa tcccttcttc agcattcagt accatcaaaa cgatatcaga
     4261 acggtcaatg gcacgcattg cacgcatcac gctgtatttt tcagtatttt cgtatacttt
     4321 tccgcgttta cgcataccag ctgtatctat cattaagaat ttctgaccac tttcagaaac
     4381 gaagtgggtg tcgattgcgt ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc
     4441 ttctcctaaa atcgcattga tcaaagagga tttaccaaca tttgggcgac caatcaaact
     4501 aaacttaatc gtgtcgtcgt cctcttcttc agtctcttca gagaaatact tcactgcttc
     4561 atctaaaata tcaccaatcc ctagtccatg gcttcctgaa acaggataag gatcgcctag
     4621 ccctaaggaa taaaattcat agatatcgtt tctcatttct ggattatcta ctttattgac
     4681 tgctaaaatc actggtttat tacttctgta taaaattttc gcaaccatct catctgcgtc
     4741 tgtcacgcct tcgcgtccgc tgactacaca aatgattaca tctgcttctt caatcgcgat
     4801 ttctgcttga tgcttgatct gatccataaa aggttcatcg ccaagatcaa ttccgcctgt
     4861 gtcgatcacg ctgaattctc gacctaacca ttcacctttt gcataaatac gatcacgagt
     4921 tactccagga gtatcttcga caatcgagat acgctcacca gcgatgcggt taaaaatcgt
     4981 agacttaccg acgttcgggc ggccaacgat tgcaattgtt ggatttgcca tattatttcc
     5041 tcctcacaag tttccaatct cacttagttt aacgaatgca ttgccaactt tcaagcatag
     5101 attgtcaatt aaacgaagta tttttattcc ttattgaaat actaatcgct tttcaaaaga
     5161 aaaactgaga caaaagcagc tgctttcatc tcagtcacag atacaaaatc aattattttt
     5221 cagaatcatc ggttgctttt tcttctactt cttctttcaa cgcatcacct aaaatgcctc
     5281 ccatagtaaa tccagtattt tcttctggta attcataaga ctcaacattt tttggttctt
     5341 cttctgaagc tggtttttct tctaaagctt tgatactcaa tgcaatacga tgttcttctg
     5401 gatggatctc taatactttc actttcactt cgtcaccttc atgaagcact tcatgtggtg
     5461 tcgcaatatg tttatgtgaa atttgagaga tatggactag cccttctact ccagggaaca
     5521 cttcaacaaa ggcaccgaag cttgtcaaac gtttaactgt tccgtctaat actgtgccaa
     5581 ctggagcttt tgtatcgatt tctgaccaag gtccaggctg tgtgtctttg attgacaagg
     5641 aaacacgttc ttgttcagga ttgatagaca acactttgac atttacatca tcattgacag
     5701 ccaatacatc acttggtttt gctacatgac tatgtgaaat ctctgagaca tggactagac
     5761 cgtcaattcc gcctaggtcg acaaatgcgc caaaatcagt aagacgtgca acacgtccac
     5821 tgagaacatc tccctcatgg atagttgaaa gaatttcttt tttcttttct tctctttcgt
     5881 tttccactac cgctttatgc gataagatca aacgattttc tgatggttcg atttcgatga
     5941 ttttaaagct caatgttttg cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca
     6001 ccattgatgc agggacaaaa ccacgtacac cgacgtcaac gactaaaccg cctttgacaa
     6061 catttgtaac aggtgcttcg atgaccttgc cctcttggaa atctctttcg atatcttccc
     6121 aaactttttt agcatctaaa cggcgttttg aaagtagata gctgccgttt tctttatctt
     6181 tgccgatcgt actgataacg actaaatcta atacatcacc gacttttact acttcgctga
     6241 tatcttctac cggcaaagtt gataattctt ttgccggaac gactccttca actccagccc
     6301 cttcgattcc gacaatcact tgtttgtctt caatgactaa aacttcgccc ttaacaacat
     6361 cgccaacttt tacttcttgt acgcttttca ttgcgtcttc cattgactgg ttgttctctt
     6421 gattttgatc aaattctgtc ataacacttg tcctcctaac caacattctg ccgtaagtac
     6481 ggtactgttt ttagtttaat tgattattca aaaaaaataa agcgatttcg ttcgttttcc
     6541 ttgaaaaaac aattgttttt catttttttt cttaatttaa aaaagaagat aacctttttt
     6601 caaaacaaca gcttttattg ctgcaacgac ttctggaatc gatttgccag tcgtatcgat
     6661 acgaacagca tcctctgctt gcttcagcgg agagacgtcc cttgttgaat cataatggtc
     6721 tctttcctgt atctctttcg tcagtgtttc aagatcagtc ggaattcctt tttcttggtt
     6781 ctctttgaaa cgtctttctg cacgctcact tacgcttgct actaaaaaga ttttcacttc
     6841 tgcattcggt aatacagctg tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc
     6901 ccctattttt cgttggacag caactaattc ttctctgact tttccatgtg cagagacttc
     6961 agaaacagct tttgtgacat ctggttgacg gatttctaaa gtcacatctt ttgatgctat
     7021 gaatacatgc tgtccatctt cttgttgcga aaaagagata ggatactttt tgatcagcga
     7081 aactagccct tcttcatctg aaaaagatac attgttcgta atagctaaat aagttactgc
     7141 gcgatacatg gcaccggtat ctgtataaat ataattcaat tcttttgcta atatcttggc
     7201 aacggtactt ttcccagatg aagcaggccc gtcgattgca atactgattt gtgtcatgat
     7261 ataactcctt tttctcagaa aaaaagccgc aaagcgcggc tattattatt taattcttaa
     7321 ttcttgacca gggtaaagca taaagttgtt tggatctaag ccattcaatt caaacaattg
     7381 atcagtggtg atccctgcac gctcagccac ttgttttgga ccttcgcctg cttgaacagt
     7441 cgttgttgaa ccggctgcag ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga
     7501 atcccctgct tgataagttg cctcacttga ggaagtcggt gttgtctctg ctgcaatact
     7561 gctttctgac gttacacctg tatcttgaga cacagtagat tcttccgcga ttgtactaga
     7621 ttctactgtt gaagtctcag ttgactcctc ttttgttgta cttgtagaac tttctactac
     7681 tgatgtcgaa gtttcagaag aagttgctgc tgttgcattg ttgcttgatc catttctgat
     7741 ccagaaataa gcacctgctg gaatagcgat acataagacc agtaaaatca aaaatagcga
     7801 aagaaataat gtatttcctt tcttttgttg tctctgagaa cttcttgatg aaacttcatc
     7861 atctgtatcg tagattggtt gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg
     7921 atgtctatct tttctgctca ctgttttacc tcctaaactc tatcgtgtgt attataccat
     7981 aggaaaaagt gtcttgtcct aaaaaatcaa tgttccatgt tttttttaaa taaatttagt
     8041 aaaatcgttt cccaatgaag ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt
     8101 tctgcttttg ctccatcaat atcacaacaa ttctgcggct tttcggagag tttttcgcca
     8161 aaataagcta agatgaattt tctgcggcag ttttcttcat taatatagcg aagcatctgc
     8221 tgcaggcgga attctttttc tttttcctga cgtttgagcc gttccaccca attttctggt
     8281 ttttcagact gctggatcag ctccatccat tttttttgca gctcatcaaa gggagcttgc
     8341 tctgtggcat attccaaata aagctcaaaa ctttgtcgct gttctctaga aagttgatta
     8401 aagaaataat ggatacgttc atccccactt tgataaaaaa gaattgctgc actttcttcc
     8461 tgatctctgc ctgctcgacc gatttcttgt acatagtttt ctagactgtc tggcaaatca
     8521 taatggacca cataacggat atcagattta tcgatcccca taccaaatgc attcgtagca
     8581 atgagaaact gcagctgatt ttttacaaac tgctgttgaa gttgtcttct ttgagctgca
     8641 tcaagaccac cgtgatagta tccaactgta aaacgttccc tgaataaatg atacaatcgt
     8701 tcgacttctt tttttgttgc acaataaatg attgccgctc cttgtgctct ttccatgaag
     8761 tgttcaagtt cttgttcttt ttgctgcgtt ttccgaacaa aaagcgagat attttgccga
     8821 ttgactgatt gtctgatctc ttttggtggt cgatcaaata gtacatgacg tatatctctt
     8881 gctactaaat ctgttgctgt cgccgttagt gccagtgtga ccgggtttcc taaaagcttt
     8941 cggatttttc ctaactgttg atattctgga cggaaatcaa ctccccattg agacacgcaa
     9001 tgagcctcat ctactacata taacgcaata ttttgttttt tcagatgctc taaaagagaa
     9061 ggattagtca acatttcggg actcaaaaac aaaaatttat attgcgatag atgacggagg
     9121 acgtatcttc tttcagattg cgaaagtaaa ctattgaaag cagctactcg tttctcccct
     9181 cgtttttgta attgcgtcac ttgatcttcc attagagaaa gcagcggtga cacgatgatg
     9241 accagacctt ccattaaata ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta
     9301 ggcaaaatag cgagagtatc ttctccatca agcaacgcct gaataatttc ttcttggccg
     9361 ggacgaaatg aatcaaatcc aaaatatttt tttaattcat ttattaacat tctgtatcgc
     9421 ttcctttctc aatagataga tttgatacaa acgaaattct ccataatcta attgtccatc
     9481 tacattccat tcgctaaagc gccactctcg cacggaatcc ttatttggtt ccaaacgttc
     9541 gattgtttct gcagagagaa tccactcgta tggaaaatca tcaaaaaaca aggcccactc
     9601 aataagatga tctgttactg ttcctctttt taaatgacgg attgccatga tctggtcaat
     9661 tgtctgacca ttcataaaca tctgcttggt gatcatcatg ctttgattga aattttgctg
     9721 taataatgaa tcgatcaaag cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa
     9781 caaaaggtgg atacatctgc tttgttgcag ataaaccgct gactcatcat tgttttttgc
     9841 cagctgataa ggaagcagcc ctgttcgctc atttccagat aattggttag ctaaaaaatc
     9901 tgctgcttct tctggtatct ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat
     9961 caaaatttca cgtggcaatt ggctttcact aagccatttt tttagttgga aagtataaaa
    10021 aggacttgtc tctgctggaa gatagtcttg aatacccgtt gccagattag aaattacttg
    10081 aacagcaaat ttaatgagcc gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg
    10141 caaaccagta tgttctattt gatgttcgga caacatatca gcccctgcag gcgttagctt
    10201 tgcttcattt tcatttattt cgatccaacc ttgctgcgca attttttgca aaatttgata
    10261 aaacttatcc tgcttcaatt ctggaaaact gttatggata aacaaaagtt cgtgggtaaa
    10321 tccataaata agtactgaag tcgttcgttt tcccacaagg agttggtaaa gagaactaat
    10381 ccgcagcttg tgtcctgatg aaaatagcga taaaataaaa acaatcatat tattctcctt
    10441 aagttaggat ggttagaaat gtcactacta tgtaaacttg tacctgaacg ctgtattgca
    10501 tgcggtcttt gtcaaatcga agcgccagat attttcgatt atgatgaaga aggaatcgtt
    10561 ttatttgccc aagaaccaga ggctcatcaa cagttcgtga tgccggaaga agaaacatat
    10621 gtaaaaaaag cgtatcaaaa atgtccagtt cgagcgattt tattagaaaa taaaatataa
    10681 atataaacga aactttaaaa acgtacacta agttctctaa acttgaaaat aagccgggat
    10741 ctgtttctct tgttccgtga aatcccgact tattttgaaa gattcatttg ctatctatag
    10801 ttaattgact agttttttca cggaccctat aggacccatt tatattattt caactttttc
    10861 agccagtttt ccaatgtatt gacgatcaca gtttgctggt ctgcgttaga aatttctgcc
    10921 tctccatctc ctttttgctc accatagctg ccaaaacctc catgatttcc gccattgatt
    10981 gatacatact ctgttctttc cggtaaatac tgcttagctt cttgataagc ttcctgattc
    11041 aatactttat cttttgtcgc cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt
    11101 ccttttttgt cgggataact tgccatgaag aatacccctt ttaaatgttt gtctgaatga
    11161 ttcttattga cataacgact tgccattacg ccacctaatg aatggccacc gatcacataa
    11221 tcggaatttg gaaaatcttc tttgacctta tccgctttgt ttccgcccat cactgctaga
    11281 tcaagtggca ttttgacaat agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa
    11341 atactataac tttctggctc tacgagagca ccaggataaa aaataagtaa tggagacttt
    11401 ccatgattct ttttctctcc ttcaaaaaat aagtaatctt ctttgttttt tccttctttt
    11461 accaacactt gcgcttctga cgatggttcg tacgtatttt gctgtatata gtaataaccg
    11521 gctccaccga gtaatcctaa aatgatgact aagccaatta gtattcgtct aaatattttc
    11581 actttctccc accttttcta tctgagtgac ccctttttct taatcaagta tgattatacc
    11641 ttaaaaaaat aagagagaaa tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc
    11701 catttgaata tccgcccttt ttgtatgttt atttttattt agcacataaa cgatacacag
    11761 cttctgcata aatttccatt gctttataca tactacttaa ttcccatcgt tcattcgcct
    11821 ggtgcataaa gtcaggagta tcagggaaca tcgcaccgaa tgccacacac tggttcatcg
    11881 tacgagcaaa agtagcgccg cctgatacaa acggttcagt catgtctcca gtaatatcgc
    11941 gatatgtccc caatagattt ttcactaact cactgtctag tggcacgtat aatgaatcaa
    12001 ggaagtcaaa ttcttcataa gtcaacccat atttttctgc tgttttagtt aatttagcta
    12061 ctaaatcatc tttcttgaat gtaacaggga ttctcatatc tacaccgatt tttgtttgtt
    12121 ctggtgtgat ttccaaactt gcaaagttca tcgttagctc gccggattgt tcatcttctg
    12181 ttttgcccac gacagcttca ccagttgcat tttcttgaac taattttcct aagaaattga
    12241 ttgggccaaa atcaaatact tctgaaagag caattgccag acgagaaata gcattcactc
    12301 cttgtgctgc atctttcgcg tggatacttt taccaagaac gacgatgcct tcaccttgtt
    12361 cttcgaaatc gaattcgtgt tttttcaatg cttcttttac ttcagaaagc ttttctccag
    12421 agtaaggagc tgcatcagga acaacattca acgctcctcc agcttttaca gaaaactcgt
    12481 tcgttcctgg tcctgtcaag taagcttgca ataagccttt ttctgcataa attaaaggaa
    12541 attcagcatc tggcgcaaat ccttgcgtaa ttccttcttc tttttcgtta tatttttcta
    12601 aacaacgcca taaattttct tcatctgtac caaagataaa acgaatacgt gtcttgaatt
    12661 ccacgccagc atcaatcaaa gctttgacgg catacattgc cgcaatagaa ggacctttgt
    12721 catcttggga tccgcgacca actaaccagc cgtcttttat agttggatcg aatggttttg
    12781 tttcccaatt attttcgtcc cctgctggaa caacgtccat atgacacaag ataccgaata
    12841 attcatctcc agagccaatc tctgaataac cgtaatagcc ttctggatct ttaaatgttc
    12901 taaatcctag cttttcactg atttccaata ctttatccaa agcttcaatc acttttttac
    12961 cgaagggatg tccttgtcct gaatctgctt catctagtac ggaaggaatt cggatcaatt
    13021 cgctcaatga ttctaatgct tgttcatgat gttgttctgt cacaaatttt ttcatgatat
    13081 tatcctccta atatgttaaa aagggtcatg tcaaattcct ccgacacaac ctcttcttct
    13141 caaataatga cgttttaagc ctgctccatc agcaagcaga atgttcttaa caaatgatga
    13201 aagaaaaaac actcactgaa tggagctatt gacttatcca cgtgcttatc gatagccgtt
    13261 tatcggtttc ttcattctta tgatagacct tgtacctgta tttgtctagg tttagaacat
    13321 tgctgctaca cctaggatca cacagatggc tacaaataat atcgcaatta atttaccggt
    13381 aaatttccac cacactgaaa catcgaaacg agcaattgct acagcaccca ttacgatagc
    13441 agatgttggt gtaattaagt ttactaaacc agatgcagat tgataagcag tgattactaa
    13501 atccttaccg acgccggcaa attcacccat aggtcccatg attcccattg ttgcagcagc
    13561 taagccggaa gttgaaggaa tcaagaatga catagggata tagaaaataa atgtaataat
    13621 gatgaataca cttgaagata atgaacttaa tccttgttcg ccccaatgta agatggtgtc
    13681 tgtgattaat ccattgttca taaccacttg gataccacga gcaacagcta ctacgattgc
    13741 tacaccgatc aaatcagaag caccagctaa aaattcagag acaaattcag actctttcat
    13801 gccataagtg attgctacta cgatggacat tactaagaat aacatagtaa tttctgggaa
    13861 gtaccagtca cctaatggaa gcatgctttt acctagaacc gtacctagta ccggaaggtt
    13921 tgttaaccag tttgtcaatg attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg
    13981 aatcaaactg atgatcatga taccaaaagt aataaagaac aatacattaa catgtttttg
    14041 ttttttcgtg attacttctt gacgaccgat tgcttcaata tcgaaatgct tcatatcttc
    14101 tttacgttgg ttgtatacta atgattttgt tggatctttt tcgatctttg atgcataacg
    14161 atacacatat acaatagaaa tagctaacaa aataacgaac atcaagaatc gaagaccaat
    14221 accttcaccc atcgaaatcc caacagcttg tgaagctacg cctgttgcaa aaggatttac
    14281 cgttgaagct agacagccta cttgagaccc tactaatacg attgctacag ctactactgt
    14341 atcaaagcct acagccatca tgactgggat cagtaaagca tagaatggaa tcgtttcttc
    14401 tgccattccg taagtagagc cgcctaatgc aaataacagc atcaatactg gaataagcat
    14461 tttttctttt cctttgaatt tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt
    14521 tttgttgata atacctaaaa atccaccaac aaccaaaatg aagaaagaga ttgggattgc
    14581 agcaggtgtt tctttagtta gctcccccgc tggtacacct agcatcccat tgattggcgc
    14641 catgaaaata tcccacagcc cttgaggatt ttgttgtact cgttcatatg atccagcgac
    14701 gatgttgcct gcatcgtcta cactatagtg cccagctggt ataaaccaag taagtacagc
    14761 gattactgca ataataataa ataagaccgt atacgctgaa ggcatttgaa attttttctt
    14821 ttttgtttgt tccatcttct tcttcctttc ttttccgtta gagacaacaa ttttggctct
    14881 aatgttattt tatcaaaaag agtatcagaa caaaaataat aacacgcatt ttgtaaaact
    14941 tttgttcaaa aagttcgttt ttccctattc cctcctcttt tttcaggttt ttaaagcttt
    15001 cttctttttc attgtagcgt ttcctttttt ttataaacaa acattttcgt cttttaaaag
    15061 gagaagaatt tcacatttgc aaagaattga ttgattttta tgcataaatc aaaaaaatac
    15121 tcaaaacaaa caaataaaac gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa
    15181 aagaccaaga aatttcttga tctttttgat atttatttga ctgctgtatg acgttgttcg
    15241 aactgatgtt gttttcgact taaccatgga agaagttgcg catgcagcac taaaaagaca
    15301 gcactcacga tgaatccttt gatcaagttg aaaggaacga tcccaatcag tacatagttt
    15361 gctaaagaca tgcccagcat ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc
    15421 gtaataacga aataatttgc gatactcata aaaatagtta gagcgatggt tcctgtaatg
    15481 acacctaaaa atttgtttct tcctttatgc tgtttcttta cactaaagaa ataataaatc
    15541 gggaatgtaa agatcgttgt tgataagaaa ctagcagtgt ctcctaccaa attatccgga
    15601 gaaaaacctg tagtcagcag atgtaatact gaacgcaaga acgctgttgc aactccggcc
    15661 gctggaccga ataagaacat gctgatcaat accgagatat cactaaaatc gatcttcaaa
    15721 aaactaaaag ccggcatgat cggaaaagca aaatactgta aaactaatgc gatcgcagca
    15781 aacatcgcta ctgcaaccat tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt
    15841 aggactcctc ttcagcgaac tttttgacgt ttctctccaa atgaaaaaac tctattttcc
    15901 agggaaaata gagttagagt tatttgatca agcgtcgtca aataagctct atcttcttta
    15961 tccagactat actgtcggta tcggaatttc accgattcag ctattttttt gcaaaatagg
    16021 tcgtggacta taaccaccgg tcgggaattt caccctgccc ctgaagacga accttatata
    16081 atttttgttt acatattcat tatacatatc tattcaaaaa acgcaacatc taacacttat
    16141 aaaaagtaag tgttcatttt gagtggttca gcaaactgct gacttctttt ttgttcagtt
    16201 cacggtaatc cccaggacgt aggccttgta aagtcagatc tccgtatttt tcacgtttta
    16261 gtttttgcac aggaagacca actgcttgaa gcatattttt cacttgatga ttacgtcctt
    16321 cgtggatcgt caattccacg atactgtggt ttgtcttagg atccactgat aaaatttgga
    16381 atttagctgg agatgttttt cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca
    16441 tcgattttgt agcgatccct tttacctttg caacgtaaac tttatccact tcatgttttg
    16501 gatgtgtcag gcgctgagca aaatctccat catttgtcag tagcaagatc cccgaagtat
    16561 cgtaatccag tcgacctaca ggatagatac gttcttttac catcggcaaa agatccgtaa
    16621 caacttttct tcctttgtca tctgcaacag cagaaataac tcctttaggt ttgtacaata
    16681 aatagtaacc gtattcttct tgataaattg ggacttcatc cacttccaca gtgtcatgtt
    16741 tcccaacttt tatccctaat tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa
    16801 ggatgaattc ttctgctttt cgacgagaag tgatgccagc gtgggcaatt actttctgca
    16861 atctctccat tacaattatt ctcctttatt cattccatca aagaatagat ctggtgacac
    16921 gtcttcttca agtgaaattt ccatttcttg tgtatcaggc agttcttcta atgatttcaa
    16981 accaaaataa tctaagaagt agccagttgt tccatataat atcggccgac ctggtccttc
    17041 tactctacct ttttcttcaa tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac
    17101 ctgaactcct cgaatctgct cgatttcagc tcttgtgatt ggctgtttat aagcgatgat
    17161 tgctaaggtt tcgacggcgg cttgagataa tgtgtttgat gtcccttgcg catatcgctt
    17221 aagtaaggga gccagctctt ttttagtcgt taatacataa tggctttccg tttcaaaaat
    17281 atgcaaagct gagtgcgagt tttgagtgta agattctttt aattctttca ctaaacttgt
    17341 gacttctgct tcgctgagat ttaacagtcc gcttaattct tcattactga tcccttcctc
    17401 gccaacaata aaaagaaggg cttcaagttc acttaatttc gtcatgttcg gtatctccta
    17461 ctgctggata aagcataatt gattcatagt tttctttttg ttgcgcaatg atctgccctt
    17521 ttttcatcaa ttctagcaaa gccataaaag tagtgacaac ttcactttta gaaggtgact
    17581 caaaaaaaga atcgaaagta cagccatgcg catttctttt tatacgctct tcgataaata
    17641 ccattcgttc ttcgatcgtt gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa
    17701 atttcttacg ttctaagatt ttctgcatgg cacggatcaa atcgattttt ttgacttgtc
    17761 ctcgcttcaa cggcttttca gcatccgcta acccatcgat gttcataggt tcttttgtgt
    17821 aataaagact tcgttcctct gctttttctg tcagtctttc agctgcatat ttatatttac
    17881 ggtattctaa aagctgagtg accaattctt ctcgaggatc ttcttcccat aattcttcat
    17941 cgtctgtgac gaattcttgg acaggaagca atgtcttgct tttgattgcc atcaatgtag
    18001 cagccatcac taagtattct cctgcaacag ctagctcgag tgttttcatt gtatggatat
    18061 aattcatata ttgctccgtg atcgtagcga tcggtatatc atatatatcg atttccattt
    18121 gctgaatcag atgaagcagc agatcgagag gaccctcgaa tacatctaat ttaatattga
    18181 tctccgtcaa ctcattcact ctttcttctt tttctttgct agccattttc caatgtaagg
    18241 ctctgtgatt tcagttgcac ggatggagac atccgggtac ttttcctgta attcatctaa
    18301 catttgaaag cccatattct caccgcgata agacggattc aaagaaatgt catggatgat
    18361 cagctgatcg ttcgttgccc agtaaatacc aataatccct tgaatgttgg ttgaaccttc
    18421 tggcgtaaag aaaaaaagtt cataatcgtc ttcagattcg tacgtatcaa tctccttcaa
    18481 caaagaagtt ttacctgtca gtttcttatg gaaactcaat agtcccatag caattttacg
    18541 gttttctttc cggtaatgtg ttaacatctt ctcaccttcc tatgactaag cccttggaaa
    18601 atgctgctta taaacatccg tcattctttt cttcgtgata tgtgtataaa tctgagtcgt
    18661 agagatatcc gcgtgaccta acaattcctg tactgtccgc aaatcagctc cattttctag
    18721 cagatgtgtg gcaaagctat gccgcagcgt atgtggtgtt acttctttgt aaatcccagc
    18781 ctctcgtact aactgcttca gatttttcca gattccttgc cgagaaagac ctttcccatg
    18841 attattgaca aatacatgca tttcagatgc gtcctttaca agtaatggac gtgcttcgtc
    18901 taagtagcgt tccagccatt ggatcgcata atcacctaaa ggaatgatcc gctctttatc
    18961 tccttttcca actgtctgta ccagtccaag agaaagatga agatctccca atttcaatcc
    19021 aatcaattca ctgacgcgca tcccagtagc atacatgact tccaagattg cacgatctct
    19081 tatccctaac gtttttgtcg tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt
    19141 caatgtactt ggtaatttct gtgctttctt cggcgtatcg atatgttgca tcggatcatg
    19201 atcagtgata cgctcttgcc gtaaaaactg atggaatctt ctgagacttg agatcatccg
    19261 agtaacagtt gctgtcgctt ttttttcttc atgcaacttc tccagaaaac ggatgactaa
    19321 aaagcgatcg acatcctgcc atgaagcaat tttctgctcc tgtagaaaat gcaagtactg
    19381 ttccaaatca cgttcatagc tttgtcgtgt attggcggac aatccccgtt caatattcaa
    19441 gtagtggaga taatcgttga tttgctcttt cataataatc cccctcatgc ctctctatca
    19501 tatcagatac cgcttcattc tgatagtctt tttccataat ctgagcaaag aaaaagccgt
    19561 ctagacactc agacggactg gtttcttttt tctttttcat agcattcctg acagattcca
    19621 tggaacgtta gccgatgatc ttttactaaa aaatgataac gttcttctac tacttgctcc
    19681 acttcaagta aaagatcctc ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa
    19741 tgatgatgga aatgtttggc gccttctttt ctcaaatcat aacgggctaa tccatcatta
    19801 aaacttactt tatcgacaat tttcaattct gtcaagattt caagggttcg ataaacagta
    19861 gcaagtccaa tttccggact ctttttttca ctaaaaagaa gatttcttct gcagataaat
    19921 gctctttctc attttccaaa agcacaagca gagtagcttc tctttgcggt gtcagcttaa
    19981 accccgcttc atgtagctgc tgttttgttt ttttcatcgc cgaaatagca ttcatgacca
    20041 tttactcaac cccaacaatt tataataatt acaaataaaa aaagatataa cactatgtta
    20101 attaaaatta ttataagcta gttaagaaaa acatgcaagg gattctcaat tagatttttt
    20161 gactagtttt gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa gattacctaa
    20221 tgcgcgctta aatgctcctt tgctgatacc aaaagctttc atgatttctt ctggatccga
    20281 tttatctgta aagttgattt gctgatctgc agaacgttct aaaatggtta aaatcatttg
    20341 tgcatcatct gatatagctt catatgctct tggcttcaat gaaagattca gtacaccgtc
    20401 tggtcggaca ccaatcactc gaccttttat ctgctcgcct agacgaggtt cattatatcg
    20461 ttcagaagga tggataaacc caagatagaa atcttccgtt aaaacgtacg tacctgttaa
    20521 tttcaagcgg tatgctgttc ctatcacatc tttgtttttc atttcttctg ttccatattt
    20581 gcttaaagat ttgaatattt tttcatctgc taaactgccc caaatacggt cttttgcatc
    20641 aactctaagg gaaatcatca agcgatcccc ttttttaggc catagctctg tcattgtcgg
    20701 caattcatca agagatacga caaagtcttt atctggaaga ccgatatcga caaacgcacc
    20761 taaatctttt cgtgtagcag taacgattcc aaaaccgtaa tgaccgatac ggcttttagg
    20821 aatttgtgta gtgatccgat tttcatgttt ttgattttgg taaccgaatc cttcaactgc
    20881 ttcaccgatt ttgtgttcac cttctgattt gcttaagcca aaggtttgtc cgtttttttg
    20941 gacaaagtag tttttttcat tttcatcaat aataatacct gtaaatatag ttgctaataa
    21001 atcgttcatt tttttctccc tctatagtga aaggttttct taatacattt ctttgctatc
    21061 ataacataat gttatctact tgaatagctg agccttggtt tggaaaggtg aaaaaaggag
    21121 tatgacaaca gttgtgtcat actccccttt ttcgaataaa cggtgttaaa cggcaggctc
    21181 acagcctctt tcatttgcct aaaattaatt gctcccattt cctccgaacc atccagtttt
    21241 acagttgatg gttacgatgt aaaatggcaa agcactttct ccaatgcctt tcatcaatgc
    21301 agattttgtt gaagtggcat atagattata atagccttcc ccattttcga ttctggcttt
    21361 ttctaatctg taacctcctt caacgatata tccacgttgg atacatgtag caatcacttt
    21421 gctttggaca cctggtgccc acgtccatgc tggattagat gcatcggcaa tatcacttgc
    21481 tctggcagat ttgattggct gattcgctga gtttgaagag gaaggggtcg aggatgccgc
    21541 ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg cagctgctgc
    21601 ttgagcggct tgttttgctt gttcttcagc tgcttttttc gcttcttctg cttttttctt
    21661 ttcctcttct tcttttttct ctttatcagt gagcatctta tctacttgtt tcaattgttc
    21721 attcaactga gcagatagat ctgtattttt cactttttct actgcttctt ttgccgtctt
    21781 gtattggtcg cgtgttgctg aatccactac ttttccgtct ttaaagacta tagctgtttt
    21841 ttctttagct gtttgcaatt cagcatattg atttttagct tctgtttgtg cttgttgaat
    21901 caattttcca aaatctgtgt tatccgtttc gattgcttgg atctctttat ctgctttcaa
    21961 cttgatgtca tctgctagat gatcatcgac gatggcaggt gctatgaacc actcattcac
    22021 tttttggata tctcggatct tagattggat atcttggtaa gttgtttcta actctgaata
    22081 tcctttttca ttcttaagac tggataaatc attttttagt tttgataaat cttgatgaat
    22141 catatctgca cgaataaatt gatggtgatc atctgtataa aaggcagcaa gtttctcatg
    22201 aagttcttcc atttgttgct gtctttttgc ttctgctgct acttcggctt ctactttttg
    22261 atgatggtca taataagccc agcttcctcc accgagtaga agtactgctg ctaaaacgag
    22321 ataaggtact ttttttgatt ttttcttttg cgctttatct ggatctgttt tttctaagtt
    22381 tgattcttga tgtgtttttt cagtatctgg cacgattgtt attgatttcg cttctgcttc
    22441 aaacagctta ggctggttct cttctttttc tatttttgct tcaagatcag taccctcttt
    22501 gcttacagat tgattttcag catcggattc actatttatt tcatcgggct ctgttgactt
    22561 tttgtctgtg ttttcgagta tttctttttt ctctgcttcc ggcagtttat tcgttttttc
    22621 cttttctaat tgtttttcag taaatacttc agttttttct gtgatatccg tttttacgtc
    22681 ttttattttt actgtttctt tacctgtggc gttttctcta tcagagtctt tttcagtttc
    22741 ttgttccttt tctggctcta ctttgttttc ctgtgcaatt ttttcactga tcattttgag
    22801 cagctctttc gcgtgctctt cttttgtatc ttcacgatgt gctttgatat aagcggatag
    22861 tatcgaattt tcttcctcat tatcgacgat ttctttcagt tcagctactc ttcttttttg
    22921 ttttgattca agcgtttctt cttgattctt tcctgattct tccgaagtct catgaacagt
    22981 tgctttcttc tgttcatttt tctctatatt agttacttga tcgtgagcag gtggttcttc
    23041 gtcttctggt tctgcttttt cgaataattc cattacagat tctagaggta aatctttcaa
    23101 ttccgaccat tcgattggat cgtttaattc tgtatcagga aagactgcat cttcagttga
    23161 tttatcctgc atagctgcat ctgtatttgt ttcttcaatt gattctttta tttgctgatc
    23221 ttctgcagtt atttgatgac cgcagtctgg acagatagta ttctgctcca tttttttcga
    23281 tccgcaattt ggacattttt tcatcactta gcgatcggct cctttactag gtttacgcta
    23341 ccatttaatc tatcatattc tatacgtcac gccaatagct tatttttagg atttacttaa
    23401 aaataatgaa tgaatttcct tcctatagaa aaaaaagctt gagactgcgt agtctcaagc
//
LOCUS       DASHFF010000042        22831 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.042, whole genome shotgun sequence.
ACCESSION   DASHFF010000042 DASHFF010000000
VERSION     DASHFF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.042"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="U2I75_001803"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="U2I75_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN1696928.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /locus_tag="U2I75_001804"
     CDS             complement(642..1364)
                     /locus_tag="U2I75_001804"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN1696929.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /locus_tag="U2I75_001805"
     CDS             complement(1500..2381)
                     /locus_tag="U2I75_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN1696930.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="U2I75_001806"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="U2I75_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN1696931.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="U2I75_001807"
     CDS             3621..4100
                     /locus_tag="U2I75_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN1696932.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="U2I75_001808"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="U2I75_001808"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEN1696933.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..6186
                     /locus_tag="U2I75_001809"
     CDS             5401..6186
                     /locus_tag="U2I75_001809"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN1696934.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6532..8223)
                     /locus_tag="U2I75_001810"
     CDS             complement(6532..8223)
                     /locus_tag="U2I75_001810"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN1696935.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8645..9592)
                     /gene="arcC"
                     /locus_tag="U2I75_001811"
     CDS             complement(8645..9592)
                     /gene="arcC"
                     /locus_tag="U2I75_001811"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN1696936.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9707..10726)
                     /gene="argF"
                     /locus_tag="U2I75_001812"
     CDS             complement(9707..10726)
                     /gene="argF"
                     /locus_tag="U2I75_001812"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN1696937.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10817..12046)
                     /gene="arcA"
                     /locus_tag="U2I75_001813"
     CDS             complement(10817..12046)
                     /gene="arcA"
                     /locus_tag="U2I75_001813"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN1696938.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12507..13208
                     /locus_tag="U2I75_001814"
     CDS             12507..13208
                     /locus_tag="U2I75_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1696939.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13780..14796)
                     /gene="tsaD"
                     /locus_tag="U2I75_001815"
     CDS             complement(13780..14796)
                     /gene="tsaD"
                     /locus_tag="U2I75_001815"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN1696940.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14793..15257)
                     /gene="rimI"
                     /locus_tag="U2I75_001816"
     CDS             complement(14793..15257)
                     /gene="rimI"
                     /locus_tag="U2I75_001816"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1696941.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15264..15806)
                     /gene="rimI"
                     /locus_tag="U2I75_001817"
     CDS             complement(15264..15806)
                     /gene="rimI"
                     /locus_tag="U2I75_001817"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1696942.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15790..16512)
                     /gene="tsaB"
                     /locus_tag="U2I75_001818"
     CDS             complement(15790..16512)
                     /gene="tsaB"
                     /locus_tag="U2I75_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN1696943.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(16703..17683)
                     /locus_tag="U2I75_001819"
     CDS             complement(16703..17683)
                     /locus_tag="U2I75_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1696944.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17707..19191)
                     /gene="galT"
                     /locus_tag="U2I75_001820"
     CDS             complement(17707..19191)
                     /gene="galT"
                     /locus_tag="U2I75_001820"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN1696945.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19203..20192)
                     /gene="galE"
                     /locus_tag="U2I75_001821"
     CDS             complement(19203..20192)
                     /gene="galE"
                     /locus_tag="U2I75_001821"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN1696946.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20441..20608
                     /locus_tag="U2I75_001822"
     CDS             20441..20608
                     /locus_tag="U2I75_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN1696947.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(20670..22481)
                     /locus_tag="U2I75_001823"
     CDS             complement(20670..22481)
                     /locus_tag="U2I75_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN1696948.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(22478..>22831)
                     /locus_tag="U2I75_001824"
     CDS             complement(22478..>22831)
                     /locus_tag="U2I75_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN1696949.1"
                     /translation="KRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIAPSNE
                     ARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKNEHQN
                     HAVLLKKFIEMEACK"
BASE COUNT         6387 a         4766 c         4046 g         7632 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttaatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgctcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccaccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg catccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt aaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaattt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aactcctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagcg
     4501 gctcctttgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat ggacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccctct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaaaagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgattcg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaacta gaaaagcgaa aaaatgtgag tcgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtact acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aacggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgcgaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgactggat ttactgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaggg
     5821 tattcaccag acttcatggc aacttctgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc tggcgctgat
     6121 atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga caagaatcca
     6181 gaatgacttt tatacaaaat cagaggtcgt gaaaaagctg tactgcatca agtaataaga
     6241 acagccaaac aaaaatagct cctcatattt ttcgtttggt taggcttatt accgcagatg
     6301 caagcttttg aacaccgttt attcgaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttacaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg cgcatatcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctcttcatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga catataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgata ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgtt cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgctcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttctgataa aaaaacaatc cagatggcct
     8581 aaaggagaag aaataccacc tggatagcta taaatcattt cgtaaatacg aaagacttaa
     8641 caaattaatc agctacgata atcgttcctt cttcagaagc taaaagattc tcgatatttt
     8701 ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc aacaaatgca atagctgcct
     8761 ctactttcgg aagcatgctt cctggagcaa attgcttttc atcaatgtat tgtttcattt
     8821 ctgaaacagt aacagtttct aatttctttt gatctggttt ttggtaattg acatacacat
     8881 tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat gatttcggct aatttttctg
     8941 aagcaaaatc tttatcgatt acggcttctc gtccttctaa tccagtagct gtttctacaa
     9001 caggaatccc accgccacca acagagactg tgataactcc ttgatcgacc agcgtttcga
     9061 tgacacgtgc ttctttaatc gagatgggtt taggggaagc gactactttt ctccatccac
     9121 gtccagcatc ttctttgaat gtcacagtgg agtcagcatt catttgttct ttggcttctt
     9181 cttccgtgta gaatggaccg actggtttac ttggattttt gaatgaagga tcattttcat
     9241 ccacaataac ttgagtgact aaagaaacca catctttatc gatccctttt tccttcagta
     9301 cttcgcccat ggcattttgt aaccagtaac cgatagatcc ttctgtcatt gcaacacatg
     9361 tatctaaagg cattgccggt gttttctcag aatcagcagc ttgctgctga atcaataggt
     9421 taccgacttg cggaccattt ccgtgggaga tgatcaattc atctccttgt tcgataaatt
     9481 tcactaaata ctttgctgtt tccattaacg cttcttgttg ggcttttgca cttgcatccg
     9541 tagacaagat tgcattaccc cctaaagcga caactacttt tcgtttggcc atttcattcg
     9601 tcctttcttc agttctaccg aattttctag ataacaaaac gagccagcgt tttgtgattt
     9661 tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa acgcgaggga
     9721 taaacaagtt acctaatgtt gctgccatga tcgctttgat tgagtgcata cgattttctg
     9781 cttggtcaaa ttgacgagca tatttgctgc ggaagacttc atctgttact tccatttcag
     9841 tgatgccaaa gcgttctttc atttgttcac cgtaaacagt gtttgtatca tggaaagctg
     9901 gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt tttttctacc atttccatat
     9961 tgatttggta aggtttcaac aatttgatac gttcttcgaa tttatcttct tcacccattg
    10021 atacccatac atcagaataa aggacgtctg ctccgtctac acctttatca acatcgtcgg
    10081 taatcatcaa ttgtgcaccg gatttttcag caaagccttc tgccattttg acgatttctt
    10141 cttcaggttg taattctttt ggtgcaacga tacgcatatt cgctcctaaa attgcacctg
    10201 tgactaataa agagttagcc atattgtttc gaccatctcc gcagtaagca acagtgatac
    10261 cttctactgt tccaaagttt tcttggatag ttaaaaagtc agcgatcatt tgtgttggat
    10321 gccattcatc agtcaagcca ttccaaactg gaacaccaga gaattctgct agttcctcta
    10381 ccattttttg actgaatcca cggaattcga ttccatcaaa catacgacct agtactttag
    10441 ctgtatcttc tgtggattct tttttaccta attggatatc atttgcacct agatattcag
    10501 gatgtgcacc tagatcgatc gctgcagtag taaatgcaga acgagtacgt gtagatgttt
    10561 tttcaaataa aagcgcaata ttttttcctt ctagatagtg atgtgggata ccacgttttt
    10621 tcaagtcttt caagtgttct gaaaaatcga tcaaatattg taattcttct tttgtaaagt
    10681 ctttttctgc taataagctt cttccttgga atactgattc tttcatatta aacattctcc
    10741 tttagattgt ttttatttaa gattaaagag gaaatcccga cggcagctga cagtaagacc
    10801 gccgtcagga aacaaattac ttcaagtctt cacggacaag tggttggctc atgcagcgtg
    10861 gaccaccacg gcctcgtgac aattcacttg aattgatttc taacactttg atgccatagc
    10921 tacgaagcaa ttcgttcgat acataatttc ggttataagt gactacaaca cctggagcga
    10981 ttgccaatgt gtttgatcca tcattccatt gttctcgagg tgcaacgatc tcatctccat
    11041 taccagttgg gatcagtaca agatcatcta atccaagagc agctttcatc gtttcgtgta
    11101 agttatctga atgagtaatc ttgatatcat caccatctgg ttcgattgta aagacatcca
    11161 tcttaccgtc tttactttga attgctggat ggatagtgaa tttatcgtga tctaccattg
    11221 taaatacggt atccaaatgc atcattgcac ggttgtttgg aattttgatt gctaacactt
    11281 tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa gctttcgcat
    11341 tagttcgttg agaaatccca acagctacta ctttatcgct taagatcaat tcgtctccgc
    11401 cttcgatatg atctggattt tcacgatcca accatacttc gattccttta tttgcaaatc
    11461 ttggatgatg tttcaaaata tattctgtaa acatcgattc acgacgacgt gcttcaaaag
    11521 tcatcttatt gactgtcatc ccatttccca ttgatgcgga agggtcgcgt gtaaagtaca
    11581 agtttggcat tgggtccatg taaaatggat agtcatcgtc tgctgacaga tcgtatagac
    11641 tgctagaacg tacttcaata tcttttgtac gaaccccagc catgatttta tctaccattt
    11701 cttgcgtttc cattgataga aggaactcat gaagaccctc tcttacagca ttcgatgcaa
    11761 tatgagattc atccaacatt ttattcaaga attgttcttt gacattgcct gcgtcaatcg
    11821 cttctgccgc taatttttcc aagtaaagag tctcgacgcc ttcattttgc aatgtctttg
    11881 caaagttgtc atgttcttct tgagcaatcg gaagatatgg aatatcatcg aacaacagac
    11941 gatccataat atctggtgtc aaattttcca cttcttgtcc aggccttttc agtaaaactg
    12001 tctttaattt ccctatttca gagaaaacgt gaataggttt atccatgttg tgttgcctcc
    12061 ttctttttga ttacatgtat aggatatcct ccttttgtaa tcactttcat aattttgtct
    12121 cattcaaaat gagacattcc tcacattaat attcgataat taaataatta aacgtttttc
    12181 tatttttaag cgtaatttat tcatttttta ttctgtatac actttcaaaa aaatatttat
    12241 tcattgaaaa aaattttttg aataaattaa aaaaataaat tatattagcg ttgttagcgt
    12301 ttactatatg tattattgtt gttttttata cacaaatcat tcgcttcaaa acaaattatt
    12361 caataccatt gtatattttt tacataataa caaattatat ttgtaaagta ttaaaacttg
    12421 tttctaatgt tggtatgtta aaactttaca aattctttaa aaaaagtgac aataaatata
    12481 gtgaacaata aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa
    12541 aactcagata tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg
    12601 aacatactgt tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca
    12661 gacaacggtt gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg
    12721 caacttatta ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt
    12781 ttggagaaga gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc
    12841 ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga
    12901 tacaaaaaat ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct
    12961 ctagcgcctt tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac
    13021 caacaaaaga tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga
    13081 atagcggcac gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccggac
    13141 tgttagatta tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga
    13201 gagaataacg cgaacaaaaa taagcagtga ccctgatatt tatcagggtc actgcttatt
    13261 ttcctcccta aaacttctga aagatccggc agttttagat cactgggcaa gactcaaaga
    13321 ttttctatgt gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgatagatta
    13381 actagaggaa gcgtcttgtc ctcatccgac ctagattatt cgatattcct cgacaataaa
    13441 cgattaatca gtgagggcaa tcagaggaac ggataatgat gaataagctg taaaaaatag
    13501 ggctttgaca aaagcgtgtc acagccctta tttcgattaa acggtgtgta cctgtcaact
    13561 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
    13621 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
    13681 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
    13741 tcttattact tgatgcaggg cagatttttc acaacctctt tattcagata aaaccaagct
    13801 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
    13861 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
    13921 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
    13981 tacacctcca gctacaatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
    14041 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
    14101 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
    14161 aaaactgaaa tcataattat cttcatggat catcgctcgt ggaaaatggt aattatcttt
    14221 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
    14281 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
    14341 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
    14401 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
    14461 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
    14521 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
    14581 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
    14641 gcggctagct acttcaggaa ctacgccacc aaaacgttta tgactgttta tttgagaagc
    14701 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
    14761 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
    14821 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
    14881 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
    14941 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
    15001 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
    15061 attactttcg agtatccaat aaagccacag atctggttat cttcaactaa aagcagataa
    15121 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
    15181 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
    15241 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
    15301 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa acctaacttc
    15361 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
    15421 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
    15481 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
    15541 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
    15601 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
    15661 cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
    15721 cgatcgattt gttcgattgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
    15781 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
    15841 tttttcttct gcttctaccc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
    15901 tggttcagct aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
    15961 aactgtatta atcatcgcac taggcaatat ttcctttatt tcatagaaga atttgtgaca
    16021 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
    16081 agcagaaata tgtacatctt caagtacaat ttctaatcta ttttctttcc agcggtatgc
    16141 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataattt tatccacacc
    16201 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
    16261 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
    16321 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
    16381 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
    16441 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
    16501 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
    16561 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaatgat acatgattat
    16621 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcacttcat
    16681 ttccccacag cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca
    16741 caataatctt tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg
    16801 ctagctctcc catccattcg gtataaactt ttacagtaga aagagagggc gttacatatt
    16861 tcgctacact gacatcattg aaacccatca cagaaatatc ttctggtacc actaaacctt
    16921 tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat
    16981 caggaacatg cggattcgtt tttaaatact gcttcactgc ttgatatcct gactcgacag
    17041 aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa
    17101 atgccataat tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag
    17161 caagcatagc gatatgttgc gctttatggg agacaaaatg ttcaatgact ttttgaacac
    17221 ttgacttaaa gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca
    17281 aaagcaaggc attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta
    17341 gtgcgatcgt tccatctgtc ggaagatcac taatggaatc ccacgtttct ttcaataaag
    17401 taacgtttgc ttcttctgcc tttttttcga ttcctagacg aatagataaa taataaagat
    17461 cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg
    17521 ctgcattttt acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct
    17581 ttgtggctaa gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag
    17641 gagaaactcc tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt
    17701 ttacgtttat aaaattgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt
    17761 gaatactcca gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac
    17821 atcggtgacc gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg
    17881 acgatgatat tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag
    17941 ttctggtttc aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt
    18001 ctcctttttg atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc
    18061 tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc
    18121 aattggcgta atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt
    18181 ttcatccgaa tatgctttcc acttttccaa tactgagtta gctgcttcaa tcaacaattt
    18241 tttctctgtt gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac
    18301 ttgcggataa tctgctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc
    18361 atgacgtcct gcctgataat gatcatgaga aagaatcgat cctccaacaa tcggcaagtc
    18421 agcattcgaa ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa
    18481 tgtttccttt gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt
    18541 gtaataagca taaggagaat attggaatcc ccagctttca ccgtctagat tcattcgaat
    18601 aatccggtga ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat
    18661 gcaaagcatg caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt
    18721 tggatctttt tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc
    18781 tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact
    18841 taaattaaag aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa
    18901 tgcattgacg actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc
    18961 ttgttctgct ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc
    19021 caataagtct acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc
    19081 tcctaccatc cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc
    19141 agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc
    19201 ccctattctt cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatt
    19261 gctttgatat ctgtgtattc aggtttccaa cccagaacct ctctcgcttt ttcacttgaa
    19321 gcgactaatc ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt
    19381 cctgtcactt ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct
    19441 aaattgaaga agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca
    19501 gcaatcaaat cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa
    19561 tcgtctccat aaacagccaa tgcttttctt tgaccaagag ctacttgtaa aatgattggg
    19621 acaagatgcg tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct
    19681 acattgaagt agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc
    19741 attttttcca tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct
    19801 tctgtgattg gagactcttt tggttctccg taagttgctg cagtagaaga gaacacgatt
    19861 ttattcacat tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg
    19921 ttgaaataca tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg
    19981 attactcctt caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg
    20041 tctccttcat aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta
    20101 tctaccacag ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata
    20161 taaccggctc cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaatt
    20221 gtgtttgttg attatatata atttaaaaaa acgaagttta cttttatggt gaaaacaaac
    20281 aaagtaaaat ttcgtaaatt catatggtaa atttataata aaacatttag taaacatttt
    20341 caaggatttt ttgcaaaggt ttacaaacaa acgtaaaact agcctttttt tctcttttgt
    20401 tccataatag atataacaaa taataaatga ggtggattca atgaaaaaat ttgtatctgg
    20461 aatattagta ggaagtctgg caacagccgc tgccgttgct ggtttggtcg cttctgtgaa
    20521 aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg atcgaagaaa accgtaaaaa
    20581 agcaatgcgc aaacgcgttt ctcgttaata attatttaaa aacacgcatg aagacttgtg
    20641 aaagaacaga taagtcatca tgcgtatttt tatttgtcag tcaatataag ttgtgctatt
    20701 ttatctgcct gtctcagatt ccatggatct gtataaggtc tagctactgc tgcagttagc
    20761 caatccacac cttcaacagg tattccatag caataaaagt cagactggat ttcccctgaa
    20821 gcatcaatca cgcgattcgt acctcgctca acatctatag cttctgaatg ataacttcct
    20881 gtattatctg aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt ttttatgaca
    20941 ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa atagcgactg
    21001 ctgatttctt tatcttgtag agaaataaca tagcggttgt gcttttgctg aatatcaaat
    21061 ggcggttcga acaaatgaaa aatacccgca tctatcagtg ctgctaattc tttcgtccga
    21121 atgactggcg gaccgatcgt taaaaagcta tttagatgag taaaccatcc ccatagtatt
    21181 tctttgtatt cctttgcaga aaaaacgtgc cattcaatag cctgccggat tggatcacgc
    21241 cagtctttta atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc tttttccgct
    21301 tcttttatct gcccttcaat aaatgctcgg ctagcttctg caaaggactc atcctgatag
    21361 gtaagaggaa tttctaaata cgaccaatcc catctataag ggattagtgc tggatgcttt
    21421 ccgagacact cctctgaaga gagggagaga aaatcatgct caaaattttt ctttggaatc
    21481 ggcaaatgat attcttcgat gattttcttg taataaaaca actcaacgtc ttttttcaga
    21541 tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat gccattgctc caggttttct
    21601 tttgttagta accgtggcca tgccattgat ccgccttgct tttggtttct tcctcgaggg
    21661 taataaggga gcccccttct tgatccactg tatacaatcg gttccttgcc tgatggatga
    21721 tagacaagtt ttcctttttc gttctcgaag cgcccttctc ttgatactgt aaacaatccg
    21781 acataatcaa aaaaacttaa gcctagacct cttaaaaaga cattttcttt aggcggtatc
    21841 ttcgaaagat cgacatctgc aggattagaa ggaagctgat acagtaaatc atttttcttt
    21901 gcgtagtcta cccacactct ttcttcttct gtctcatcat tttcccagtg accgcttgct
    21961 aaaacaagct gatcgataga atagctttgc tgatctgtct gtaaaagata accatctttt
    22021 cttttgctga ctttttttac taaagaataa acaaactgaa tagaagctgg aaattcttct
    22081 tgcaactttg agaaaaacca gcgttgatag agtccgtata aacatcgagt agattgctga
    22141 tctttttcca gacgattgat ttcttctata attagttgct tatttttggt taccgtctgt
    22201 tcgatataat ctgtagcagg tcctttactc cattgaaaca agttgggtcc ctgtgttacc
    22261 aatcctccac tggacaatgt ttcatctgta aaaagcgtca cttgttgaga aacagaattc
    22321 attaataact ccggtggctg attcaaccgc caaactcgac ctccaggacc attgggatcg
    22381 aaaagaaaaa gttcaagaga atgtctgtcc tgattattat ggagaatcct ttctgctata
    22441 cttaatcctc taggtccggc accaatgatc cctattttca tttgcatgcc tccatttcga
    22501 taaatttttt caataaaaca gcatggttct gatgttcatt ttttgcacca taaacaaaag
    22561 taatgactgg atgttctttc ataagttgac tgagtttaaa aatagcttgc ttcttttctt
    22621 cttcgaaaat caattcttgc tggtatttct ttgcaaatga agcaaatcgt tctggtacat
    22681 ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc tttcaaccac agatctaact
    22741 gcgctttttc ttttgaaatt cctcgtggcc agaacctatc cacgagtatt ctatagccat
    22801 cctctttttc agcatcaagg taaacacgtt t
//
LOCUS       DASHFF010000043        21588 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.043, whole genome shotgun sequence.
ACCESSION   DASHFF010000043 DASHFF010000000
VERSION     DASHFF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.043"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            103..1527
                     /locus_tag="U2I75_001825"
     CDS             103..1527
                     /locus_tag="U2I75_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1696950.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            1988..3097
                     /gene="pdhA"
                     /locus_tag="U2I75_001826"
     CDS             1988..3097
                     /gene="pdhA"
                     /locus_tag="U2I75_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN1696951.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            3100..4077
                     /locus_tag="U2I75_001827"
     CDS             3100..4077
                     /locus_tag="U2I75_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN1696952.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            4102..5745
                     /locus_tag="U2I75_001828"
     CDS             4102..5745
                     /locus_tag="U2I75_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN1696953.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            5753..7159
                     /gene="lpdA"
                     /locus_tag="U2I75_001829"
     CDS             5753..7159
                     /gene="lpdA"
                     /locus_tag="U2I75_001829"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN1696954.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            7321..7596
                     /locus_tag="U2I75_001830"
     CDS             7321..7596
                     /locus_tag="U2I75_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN1696955.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            7602..8369
                     /locus_tag="U2I75_001831"
     CDS             7602..8369
                     /locus_tag="U2I75_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN1696956.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            8537..10366
                     /gene="typA"
                     /locus_tag="U2I75_001832"
     CDS             8537..10366
                     /gene="typA"
                     /locus_tag="U2I75_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN1696957.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            10627..12399
                     /gene="recQ"
                     /locus_tag="U2I75_001833"
     CDS             10627..12399
                     /gene="recQ"
                     /locus_tag="U2I75_001833"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN1696958.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            12513..12815
                     /locus_tag="U2I75_001834"
     CDS             12513..12815
                     /locus_tag="U2I75_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN1696959.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            12818..13981
                     /locus_tag="U2I75_001835"
     CDS             12818..13981
                     /locus_tag="U2I75_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1696960.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            14007..17435
                     /locus_tag="U2I75_001836"
     CDS             14007..17435
                     /locus_tag="U2I75_001836"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN1696961.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            17456..18580
                     /locus_tag="U2I75_001837"
     CDS             17456..18580
                     /locus_tag="U2I75_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696962.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            18570..19007
                     /locus_tag="U2I75_001838"
     CDS             18570..19007
                     /locus_tag="U2I75_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1696963.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            18991..19329
                     /locus_tag="U2I75_001839"
     CDS             18991..19329
                     /locus_tag="U2I75_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN1696964.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            19448..20008
                     /gene="rsmD"
                     /locus_tag="U2I75_001840"
     CDS             19448..20008
                     /gene="rsmD"
                     /locus_tag="U2I75_001840"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN1696965.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            20005..20496
                     /gene="coaD"
                     /locus_tag="U2I75_001841"
     CDS             20005..20496
                     /gene="coaD"
                     /locus_tag="U2I75_001841"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN1696966.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            20486..21529
                     /locus_tag="U2I75_001842"
     CDS             20486..21529
                     /locus_tag="U2I75_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN1696967.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         7316 a         3751 c         4741 g         5780 t
ORIGIN      
        1 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
       61 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
      121 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
      181 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
      241 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
      301 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
      361 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
      421 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
      481 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
      541 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
      601 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
      661 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
      721 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
      781 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
      841 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
      901 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
      961 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
     1021 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
     1081 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
     1141 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
     1201 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
     1261 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
     1321 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
     1381 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
     1441 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
     1501 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
     1561 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
     1621 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
     1681 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
     1741 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
     1801 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
     1861 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
     1921 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
     1981 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
     2041 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
     2101 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
     2161 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
     2221 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
     2281 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
     2341 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
     2401 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
     2461 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
     2521 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
     2581 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
     2641 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
     2701 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
     2761 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
     2821 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
     2881 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
     2941 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
     3001 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
     3061 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
     3121 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
     3181 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
     3241 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
     3301 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
     3361 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
     3421 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
     3481 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
     3541 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
     3601 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
     3661 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
     3721 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
     3781 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
     3841 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
     3901 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
     3961 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
     4021 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
     4081 aagaagggac gaaacaaagt catggcttat caatttaaat tgccggatat cggtgaagga
     4141 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
     4201 gatacattac tagaagttca aaatgacaaa tcagtagaag aaatcccgtc acctgttaca
     4261 ggaacggtta aaaatgtgat tgttccagaa ggtaccgtag caaatgttgg ggatgtatta
     4321 gtagaaatcg atgcaccagg acacgaagac aatgaagggg acagtggtgt agccgcagaa
     4381 tcccaaactc cagctaaacc ggctgctgaa cccactgttg atacagaatc agctggatcg
     4441 tcaagcgaag gagtcttcca atttaagtta ccggatatcg gtgaaggaat cgcagaaggt
     4501 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
     4561 gaagttcaaa atgacaaatc agtagaagaa attccgtcac ctgtcacagg gacagttaaa
     4621 aacgtgattg ttccagaagg cactgtagca aatgttgggg atgtattagt agaaatcgat
     4681 gctcctggac acaatagtgc accgtcaact agtgcgccat cagctgaagc accaaaagaa
     4741 aaagtggaaa catcaggttc agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta
     4801 ttggctatgc catctgttcg tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta
     4861 actgcaactg gaaaaggtgg cagagtaaca aaagaggaca tcgaaaactt cttggcaggt
     4921 ggtccaagca gtgcaccagc gaaatcagaa gcgccagaag cggcagcacc gaaagaagca
     4981 gctcctgcag cagaaagcaa accagctgca ccagcaaaac cattcaagtc caacttagga
     5041 gacttggaag aacgtgtagc aatgacgcca acacgtaaag ctatcgctaa agcaatggtc
     5101 aacagtaagc atacagctcc acacgtgaca ttgcatgatg aagtagaagt gtctaaacta
     5161 tgggataatc ggaaacgatt taaagaagtt gctgctgcaa atggtacgaa acttactttc
     5221 ttgccatatg tcgtgaaagc tttaacagcg actgttaaga aatacccagt attgaatgca
     5281 tctatcgacg atgcaaacca agaaatcgtt tataaacatt actacaatat cggtattgca
     5341 actgatacgg atcacggact atatgtacca aatgtgaaag atgcagaccg caaaggaatg
     5401 tttgctattg cagacgaaat caatgagaaa gcaaaattag ctcatgacgg caaactatct
     5461 gcagaagata tgcgcaatgg aacaattacg atcagcaata tcggatctgt aggtggcggt
     5521 tggtttacac cagtcatcaa ctatccagaa gtagctattt taggtgtagg aacgattgca
     5581 caacaaccaa tcgtaaatgc tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca
     5641 ttaagtttcg atcaccgtat cgtagatgga gcaactgcgc aacaagcaat gaataatatc
     5701 aaacgtcttt tagctgatcc agagctatta atgatggaag gatgataaac aaatggtagt
     5761 tggagatttt gctgtagaat tagatacagt tgtaattggt gcgggacctg gtggctatgt
     5821 agctgccatc cgcgctgctg aaatgggaca aaaagtagca atcattgaaa gagagtatat
     5881 tggcggtgtt tgtttgaatg tcggctgtat tccttcaaaa gcactgatag ctgcaggaca
     5941 ccattatcaa gagtcactag attctgaaat gtttggagta actagtgaaa acgtcaaact
     6001 agattttgca aaaacacaag aatggaaaga aaacaaagtt gtccatacgt tgacttctgg
     6061 tgttggtttc ctattaaaaa aacataaggt tgaaacaatc gaaggagaag cgttcttcgt
     6121 agatgatcac acactacgcg tcattcatcc agattcagct caaacttatt cgttcaataa
     6181 cgcaatcatc gcaacaggtt cccgtccaat cgagattcca ggatttaaat tcggaggacg
     6241 tgtgttagat tctactggcg gattagcatt gaaagaagta cctaagaaat ttgtgatcat
     6301 cggtggcggt gtgattggtg ccgaattggg cggagcgtac gcaaacttag gagcagaagt
     6361 aacgattctt gaaggctcac cacaaatttt accaacttac gaaaaagatc tagtaaaatt
     6421 agtagaagat gatttcaaga aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga
     6481 agcagtcgat aacggtgaca gcgtgacagt gaaatatgca gtggacggga aagaagaatc
     6541 cgttactgct gattatgtga tggtcactgt aggtcgtcgt ccgaatacag atgatatggg
     6601 cttagaacaa gcgggtgtcg aagtaggaga acgaggattg atcacggtgg ataaacaagg
     6661 acgtacgaac gtacctaaca tctatgccat cggtgatatc gttccagggg cggcattagc
     6721 acacaaagcc agttatgaag caaaaatcgc tgctgaagca atctctggca aaaaagtagc
     6781 tgtagattat aaagcaatgc cggcagtagc cttcactgat cctgaattgg cttctgtagg
     6841 tatgacgatc aaagaagcaa aagatgctgg tatcgaagca aaagcatata aattcccatt
     6901 ttcagggaac ggtcgggcat tatcgcttgg taaaacagaa ggattcatcc gtctagttac
     6961 gacgatcgaa gataacgtgc ttattggcgc gcaaatcggt ggagtcggcg caagtgatat
     7021 ggtctctgaa ttagctttag caatcgaatc aggaatgaat gctgaagata tcgccttgac
     7081 gattcatcct catccatctc ttggcgaaat cgtcatggat gcttcggaat tagcattagg
     7141 tttaccaatc catatttgat ttgaataata caggcgaaac gcaactgaaa aatcagttgc
     7201 gttttgtttt ttagaattat caataatatt attatgtaaa cttaaaaata tgacaacttc
     7261 ttggtttatt accacttcct atgctacact gaagaggaat atgaaatagg agagttgaag
     7321 atgcaagaat atcagtatcc acttgattta gattggacaa cagacgaaat ggtcattgta
     7381 atgaagatgt gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt
     7441 ttgaagactt atcagcagtt taaaactgtt gtcaaatcca ttggtgagga aaaacgattg
     7501 ggacgggaat ttgaaaaagc ttccggttat tctttatatc gtacagtgca gcaagcaaaa
     7561 aaaaatccaa acaaaacttt gagaatgaac gggtgagata aatggaacag atgattgatc
     7621 atatcaagtc ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa caaacattaa
     7681 caatctcgca aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga aacatccaga
     7741 attttctaat cgaaaaaatc cattcctttg atccagaggc gaaaatattg gcagaagaag
     7801 aagggtgtaa tcaattatcg gatctcgatg gaagagtctt tatcatcgat cctattgatg
     7861 gcaccttgaa ttttgtgatt gaaggggaaa atttttgtat tatgctcgct gtttatgaat
     7921 ccggcgttgg aaagcttggt ttcatttatg acgtgatgag aaatgaactt ttttggggtg
     7981 gaaaaggaat aggcgtgtat aaaaatgatg agccattgcc tagaccagca aataaatcct
     8041 tatcggaagg cttacttggt gtgaatagtt atcttttcgg tcataataaa tatggaatcc
     8101 gagaaattgc ggaaagcagc atggggacac gtatgtacgg atgcgctggt cttgaactag
     8161 tggcaatctt gaaaggaaac cacattgctt atatttcaaa ccttagccct tgggattatg
     8221 cagcgggaaa tgtcttattg gaagagtttg gtatggtaac aagcggtctt tttggtactc
     8281 ctttgacatt tgaaaaaagg gaatactatt tagcagggac ttcttgtgca tatgcagaaa
     8341 tcgcagacct tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca caaaaatgat
     8401 aaaaacgagc aaaagattaa ttttctcgaa aaaatgaaaa cattttacta tatttcatga
     8461 aaaaagtctg ctataattag cagtcgagta aaatttgccc tgtcataaag aaccaataaa
     8521 aaaggagtta gttacattga attatagaaa cgatatccgt aacgtcgcaa ttattgccca
     8581 cgttgaccac ggaaaaacaa cattagttga tgaactatta aaacaatcag atacattaga
     8641 cgcacatacg caattgcaag aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg
     8701 tatcacgatt ttagccaaaa acacagctgt agactataaa ggtattcgcg taaacatcat
     8761 ggatactcca ggacacgcgg acttcggtgg cgaagtagaa cggatcatga aaatggttga
     8821 cggcgttgtg ttggtcgtag atgcgtatga aggaacaatg cctcaaacac gttttgtatt
     8881 gaaaaaagca ctggaacaac atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc
     8941 ttctgctcgt cctgagcatg tcgtagatga agttcttgaa ctattcatcg aattaggagc
     9001 agatgatgat cagttagact ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag
     9061 cttgtcagat gatccagcag atcaagaacc aaccatggca cctatttttg atacaattat
     9121 tgaaaaaatc cctgctcctg tcgataacag cgacgaacca ttacaattcc aagtttcatt
     9181 acttgactac aacgattatg taggtcgtat cggaatcgga cgtgtattta gaggaacaat
     9241 caaagtagga gaccaagtag cattaattaa attagacgga acagttaaga aattccgtgt
     9301 aacgaaatta tttggtttct ttggactaaa acgtctagag attcaagaag caaaagctgg
     9361 agacttgatt gctgtctcag gtatggaaga tatctttgtc ggtgaaacag ttactcctgt
     9421 ggatcatcaa gatgcgttac ctatccttca cattgacgaa ccaacattgc aaatgacttt
     9481 cttagttaat aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat
     9541 tgaagaacgt ttgatggcag aacttcaaac agatgtatcc ttacgagttg aaccaacaaa
     9601 ctctccagat gcatggacag tttctggtcg tggagaattg catttgtcta tcttgattga
     9661 aaacatgcgc cgtgagggat acgaattgca agtatctcgt ccagaagtta ttgaaaaaga
     9721 aatcgacggt gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac cagaagaata
     9781 tatgggtagt gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag atatggtcca
     9841 tacaggaaac ggacaaatcc gtttgacatt cttaacacct gcccgcggtt tgattggtta
     9901 ttcaactgaa ttcctttcaa tgacacgtgg ttacggaatc atgaaccata cttttgacca
     9961 atatctgcca atgcttcctg gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat
    10021 cgatacagga aaagcaacga cttattctat catgagtatc gaagaacgcg gtacagtctt
    10081 cgtagaacca ggtacagaag tatatgaagg aatgattatc ggggaaaaca gccgtgataa
    10141 tgatttgact gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa
    10201 agaccaaaca tctgtcatca aaaaaccaaa acaattgacg ttggaagaat ctttggaatt
    10261 cttgaatgat gatgaatatt gcgaagtaac accagaaagc attcgcttga gaaaacaaat
    10321 tttagagaaa aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg taaaaggatg
    10381 aagagagaaa ccagcttgtt ataaaaaata gctggtttct ttttttgtcc aactcttttc
    10441 tgtggagaag tcatctttgt caatggacat aaattcagaa aatacgccga aagacgttta
    10501 aaagactttt aaaaaactta tttctagaga gtttttgttt cataataaga taatgtttca
    10561 cgaaaggaga tcaacttgtc cttcgaagaa aatcgaagta aaatggcaag agaaaggagc
    10621 gggaagatga acgacttatt aaaacaatac tttggctatg atgagtttag acctggtcaa
    10681 aaagaaatta tccagaaagt cattgatcag gaaaatgtat taggaatcat gcctaccgga
    10741 agtgggaaat caatctgtta ccagcttcca gctttactat tagatgggct tactgtggtt
    10801 gtgtcccctt taatctcttt gatgaaggat caggtggacg cggcgaatca attaggtata
    10861 cccgccactt ttatcaatag ctcgttagat ggttacgaaa cagctagacg cttccaagaa
    10921 atcgatagac agcagtaccg tttactttat atcgctcctg aacgttttat catgccggat
    10981 tttatccaag caatgaagcg atggaacgtc cgtatgatcg ctattgacga ggcacattgt
    11041 atctctcagt gggggcatga tttccgccca agttatttac agatggcgaa ccagctagat
    11101 caactgccaa atcgtcctgt aattgttgct ttgacagcta cagcgactgt gcaagtagct
    11161 gctgacataa aaagactttt aaaaattcca gaaaacaacc atattcagac tggttttgaa
    11221 cgagaaaatc tacgatttca agtaattaaa gatcaaaaaa aagaacaata tctaatcgaa
    11281 tatttaaaga tcaacaaaaa tcaatctggt atcatttatg cagcaacgag aaaagaagta
    11341 gaccgtctct atcatttgct taaaaaattc gatttttccg tgggtagata tcatggtggt
    11401 ctaaatgaga atgagcgaac agaaatgcaa gaagcctttt tatacgatcg cttgcagctg
    11461 attgtagcga caaatgcatt tggtatgggt ataaataaaa gcaatgtccg ttttgttatc
    11521 cattatcaaa ttcctggctc tctagaagca tactatcagg aagctggtcg ggcgggaaga
    11581 gatggtcttt ccagtgaagc catcctgctt ttctctcctc aagatatcca agtccaaaag
    11641 ttttttatcc agcagtccca aagagaagaa gggcaaaagc aaaaagaata tgaaaaatta
    11701 aaagcgatga cagaatacgt gtatattgag tcctgtcttc agcaatacat cctaaactat
    11761 tttggcgaga caagcagtcc gtgcaaccgt tgtggtaact gtttagatga cagagaaata
    11821 gtagaagtaa cgacagaagc acagatggtt ctttcttgct taaaacgtat gggtgagaac
    11881 tatggtaaac aaatgctgat gaaagtctta gcaggatcga aagaacaaaa gctgcaagct
    11941 ttaggttttg gacatttatc tacctatgga ctgatgaaaa accaatcgca aaaagaaacg
    12001 atgcagttaa ttgaatattt gatatcgaat ggctatcttc tgaccatcaa tggtgagtat
    12061 cctgttttga aagtaacaga aagagggata caagtattga aaggacaaga atctgtttat
    12121 cgaaaggaac ccaagaaagt ccagcagcta tcggatgaag aaaccgatac actatttgaa
    12181 gtgctgcggg aactgcgaac ggatctagca tcagaagcag gcgttccccc gtatgtcgta
    12241 ttttcagatt ccacattgaa agaaatgagc cgaatacgtc catcaagcag attggagatg
    12301 cttcagataa aaggagttgg ccaaagcaag cttgataaat atggcgaagc ctttttatct
    12361 cggataaaaa atgcggactc taacgttctg gataagtaga aagaacgaat acgtaaaaat
    12421 gaataaaaac aactggttaa atctgttttt tttgctcctt atgaaaatca aatatgttat
    12481 aattaaggaa ttagaatggg aggtaagcac agatggattc tttgtcgaaa aaacttgcga
    12541 tccaagtatt aaatgatgaa gcagaacgga tcaaacgatt aatccgaaat caaaacaaca
    12601 gtctctgtat ctcacaatgc aaagccttcg aagaagtggt agatacacag atgtacggct
    12661 tttcccggca agtcggtttt gctgtccgta taggagtaat cggaaagaac gagggccagc
    12721 tgatgttaag tgagctagaa cgagaattga atcagttata tagtgaggtt taccaagaaa
    12781 tttatgataa aaaagaagtt ggcaaggagg aatagacttg cctaagaaag tgaaaaaaag
    12841 gcatctatta gactatagca tcttgattcc ctatttaatt ttatgtgtat tgggattgat
    12901 catggtatat agttctacgt cgtatctatt attagaaaac ggacaaaatc cgtccgcatc
    12961 agtcattaac cagagtattt tttgggtgct tagcttgatt gctatcgcac tactttataa
    13021 aatgaaaaca gatgttttga aaaaccagcg tttgatcatg gcagccatcg cagttttgac
    13081 gattttgctg ctgattgttg tgttttttgg taaggagatc aatggggcga aaggttggct
    13141 gcaaattgca ggattctcga tccaacctgc cgaataccta aaaatcattt ctatctggta
    13201 cctgtctctg actttatcta aaagacaaaa cagtgtacaa aaagattttc ttgggactgt
    13261 gaaacgtcca cttgcaatgg ttatcggact tactgcatta gtcgctattc ttcccgattt
    13321 tggtaatgcg gcagttatct ttttgattat tttggttctg cttttagcca gcggagtcaa
    13381 ttatgtgtat actttgattg taggagtagg aggcttttgt ctaagtactt ttacgatctg
    13441 gctgatcaat atcacaaacg gaaagatctt acccggacgt ttacagtata tctataatcg
    13501 atttgctatt taccaaaatc ctttctcaga cgaattaaat aaagggcatc aactagtcaa
    13561 cggctactat gccatgttca acggaggact atttggcagg ggattaggta acagtatcca
    13621 gaaaaaagga tttttacaag aagcacaaac cgattttatt tatgcaatcg tagtggaaga
    13681 actgggagtg attatgggga ttttgatatt agcgttgcta ttctttatga ttgtgcgcat
    13741 tgtccttgta ggtatccgtt caaaagatcc gttcaattcg cttttgtgca ttgggatcgg
    13801 cgcaatgttt ttgatccaag tatttgtcaa tcttggagga attactggcg ttattccttt
    13861 gacagggatc acgttcccat ttttaagtca aggaggctcc agtttgctga tgctttcgat
    13921 ttgtgtcggc tttgtcctca acatcagtgc ggatgaaaaa cgcaagagct taggtttata
    13981 ataaaaaaag atgtaggagt ggttcgatga aaaaagtttt agttgctaac aggggtgaaa
    14041 tcgctgtacg tgtatttcgt gcttgtacag aattaggaat taaaactgtt ggaatctatg
    14101 cagaagaaga tgaatattcc gtacaccgtt tcaaagcgga tgaagcatat ttagtaggac
    14161 aaggaaaaaa accgattgat gcgtatctag atattgaagg gatcatttct atcgcgaaag
    14221 aatgcggagc tgacgctatt catccgggtt acggtctttt gtcggaaaac ttgaattttg
    14281 cgcaacggtg cgaagaagaa ggcatcattt ttgttggacc caaactgcat catttagata
    14341 tttttggaga taaaatcaaa gcaaaagctg cagctatcga agcggggatt gcttcgattc
    14401 caggcacaga tggaccaatc gcttctatag acgatgcctt ggagtttgcg aaacaatacg
    14461 gttatccgat tatgatcaag gcagctctag gcggcggcgg acgcggcatg cgtgtagctc
    14521 acgatgaaaa gagtgcaaga gagggctacg aacgtgcgaa aagcgaagca aaagcagcct
    14581 ttggtagtga tgaagtgtat gttgaaaaat acatcgctaa tcctaagcat atcgaagtac
    14641 agattctcgg ggatacgcat ggaaacgtca tccatctttt tgaaagagat tgttctgttc
    14701 agcgccgtca ccaaaaagta gtagaagtag cgccttgtgt ctctatgaat gaacagcaac
    14761 gtcaaaaaat ctgccaggca gctgtccaat tgatgaaata tgtcgggtat gtcaacgcag
    14821 gtacagtaga atttcttgtt gaaggagatg atttttactt tatcgaagtc aaccctcgcg
    14881 tccaagtgga acatacgatc acagaaatga ttacagatat tgatatcgta acaacgcaac
    14941 tgttgattgc tcaaggtttg gatctgcata aagaaatcgg attgcctcag caagaaggaa
    15001 ttaaattaaa tggttcagcc attcaatgcc gaatcactac agaagacccg ttgaataact
    15061 ttttaccaga cacagggaaa atcgatacgt atcgttcacc tggtggtttt ggcgtacgtc
    15121 tggatgtcgg aaatgcttac gcgggctatg tagtgacacc gtattttgat tcattgctag
    15181 taaaagtatg tacgcatggt gctacctttg aaacagccat tcagaaaatg gaacgttgct
    15241 tgaaagaatt tagaatccgt ggagtgaaaa caaacattcc atttatgctc aatgtcatca
    15301 cacatcctga attccaatca ggaaatgcaa aaacaacctt cattgatagt acagcaacat
    15361 tatttgaatt tcctcgacta cgagaccgcg gaaacaaaac gatgaaatat atcggtgaaa
    15421 ttactgtgaa tggttttcca ggtatcgaaa gtggcgaaaa accattttat gaagaaccac
    15481 gtatgcctaa agatctaatc acaagaactg attacgtaac ggctaagaat gtattagatg
    15541 cgaagggtgc cgatgctttg gtcgaatgga tcaaaggaca ggaaaatttg ttgttgacgg
    15601 atacgacatt tagagacgcc catcaaagtc tgctagcaac tcgtgttcgg acaaaggatt
    15661 tcaagcagat tgcacgtttg actggagaag gtcttcctga actcttttca agtgagatgt
    15721 gggggggagc aacatttgat gtggcttacc gctttttgaa tgaagatcct tggcaacggt
    15781 tgagaaaaat tcgttcttta atgcctaata cgttgctgca aatgcttttc agaggttcaa
    15841 atgccgttgg gtattctaat tatcctgaca atgtattggt ggaatttgtc aaagaagcag
    15901 cagcgcaagg aatcgatgta tttcgaatct ttgatagttt gaactggact ccacaaatgg
    15961 aaaaaagtat ccaagctgta cgggatactg gtaaaatcgc agaggcagct atttgctata
    16021 ctggcgatat caacgatcca agtcgggcaa aatacaatgt tcagtattat aaagacatgg
    16081 caaaggaact agaacaactg ggtgcgcata tcattgcaat caaagatatg gctgggctgc
    16141 ttaaaccgca ggcagcttat cgtttgatta gtgaattgaa agaaacgaca gatctgccaa
    16201 tccatctgca tactcatgat accagcggga atggaatcat cacttattct gctgcttcaa
    16261 aagcaggagt agacatcgtt gacgtggcaa tgagtgcaat gagtgggaat actagtcagc
    16321 ctagcatgag cagcttgtat tatgcactgg tcaatggtcc acggctgcca gaaatcacga
    16381 ttgaaaatgc acagaaatta aaccattatt gggaagatgt gcgcatgtat tacaaaccgt
    16441 ttgaaaacgg cttgaatgct cctgaaacag aagtatatat gcatgaaatg ccaggaggac
    16501 agtattcgaa tctacagcaa caggcaaaag ctgtcggatt aggtcatcgc tgggatgaaa
    16561 tcaaacaaat gtatcatact gtcaacttga tgtttggcga tatcgttaaa gtaactccat
    16621 catctaaagt agttggagat atggcactat ttatggtcca aaatgatctt acagaagaag
    16681 atatttatga aaaaggcgaa acactaagtt tccctgaatc tgttgtgact ttcttccaag
    16741 gagaactagg gcaacccgtt ggcggatttc ctaaaaaatt gcaaaagatt attcttaaag
    16801 gcagacctgc attgaatgaa cgtccaggtc ttttggctga atcagtcgac ttcaacgaag
    16861 taaaaaaaga attggcagaa aaaattggct atgaaccaaa acaagacgaa gtattaagct
    16921 atttgatgta tccgcaagtc tttttggact atcaaaaagc atataatcaa tttggcgatg
    16981 ttacattact ggacacacca acatttttcc aaggtatacg tttgggtgaa acaatcaatg
    17041 tacaaatcga acgcggaaaa acgcttatca ttcgtttaga tgaaatcgga gaacccgata
    17101 tcgaagggaa tcgcgtactg ttcttcaatt tgaatggaca aagacgtgaa atcactatca
    17161 aagataattc gatcatcagt acggtccaaa cccgattgaa agctgaaccg acaaaccgag
    17221 aacaaattgg agcaacgatg tctggttctg ttttggatgt attagtgaaa aaaggcgata
    17281 acgttaaaaa aggtgatacg ttgatgatta ctgaagcgat gaaaatggaa acggccatcg
    17341 aagcacgttt tgacggagaa gtcgcacatg tatatgtgtc ttcaggagac actatttcgt
    17401 ctggtgacct tttgattgaa gtgactgaga aataataagc gaaaggtggg gaaacatgaa
    17461 aagactgggt ttgttttggc tgattctgct attagttatt gtggcattct acatggaacc
    17521 agtcttgttt ccgccaaaac ttgaagtgaa ggaaaactct gatcaacaaa tatcaaacca
    17581 aatgacaacg tggaaatacc aggagcttaa agcaactggt ttttctactt atatcggaaa
    17641 accgatcgat cagctggaaa aggattttgg ccaagcctat gatcgattaa ctagcggatt
    17701 tggttttgaa acaagatatt atgaaaataa agctagtcat ctcacatttg aagcgaacat
    17761 ccaagacagc agagtcacaa ctgttaaagt actgcaaaca gggcctgctg atatcgcgcc
    17821 gttttattta gggatgacga tgcaagatct tacgaaaatc actactatct atactaattt
    17881 cacttttgag taccatgata cagaagttgg aatcgaacta atggaagagg atatgaatta
    17941 tcgtccgcta attgcttttg ataacgacac ttttgctatt ttattttttg atcaaacaac
    18001 aaacggccta ttttcagttg tgtatttaga taaagatagt cttttgaaac tattgccata
    18061 tcaagtgttt ggtgaaggcc ttccacatta tcaagcagat aaatcggctg attgggaatc
    18121 aatcaatcgc gttaaagaac gaaaaagtac gactttatta aatatgctta gaagagaaga
    18181 cgaacttccc atatacaatc aaacacttgc ctttcaaaac cacagtcagc tgctactaca
    18241 tgattttttg acaaggcctg aggaaatatt gtcagaggaa cgtatggctg attggcaaaa
    18301 atcgctaggc agtgctcaaa cgacagtgaa attctcactg acgaatgatg aattggagaa
    18361 gttaatcagt aaacaaaaat tagaaaatac tagtgggcta tttattcatc cggcgattga
    18421 cgctactttt tcgtttttat attggtacag cgatccttac aaccatgacc gtttcatgga
    18481 cccgggaacg gattctgtag gcattgcttt ttctaaagaa aatatgttag tcttattgca
    18541 agaagaaaac ggagaaagta gtgatagcag tgatcgttaa cgaagagctg tttcaactgg
    18601 aggatcaatg cctccaagtc gctgaaatga taaaaaagag taaaacgatg gcttcttatc
    18661 ttgaacataa acagtcaatg gaagaatctg caaaagtttg ccagttgaaa gaagcgttct
    18721 tgtctaagaa agaagcattt gaaaatgtag cggcatatgg tgagtatgca cctgattatc
    18781 gtgaaaaaaa acgagctgtt cgaaaagcaa aaagacagct tgatatggat gagcatgttg
    18841 cagctttccg atatagtgaa acacagctgc aaagtctgtt ggatcaaatt gtccaagtac
    18901 ttgccgaaga agtttctaat gaaatcaaag tagatgcagg tagcccgttt tttgaaacga
    18961 aaaaacactt tggatgcgga ggtaattgtc atgcaattag acaatagtga atttgaaata
    19021 acaaaacgac gcgggctgat cgtatgggtc tacagcttaa aacaattaaa gaatctgaaa
    19081 aagttcggct tgttgcatta tgtctcaaga agaatgaaat atgtcgtgct ttacttaaat
    19141 gaagagacat tggaagaaac agaagaacga atccgtaaat tacattttgt tcgaaacgta
    19201 gaacgttctt accgaccgga tatcgaaatg aattttgccg aaaaaatagg aaacaaaaca
    19261 gtattaaaag acgaaggttt cgaaattgaa gagctaaata cagaaatccg attagctgaa
    19321 catctttaaa aatctcttat gagcagtttt taaataaaaa ataacttgga taacgatcga
    19381 tttttgatca ttgtccaagt atttttttgg tatactagta ccagtaccat ttctaaggag
    19441 tgagtagatg cggataatat caggaaatta tggcgggcgc cgtttaaaaa gtttagcagg
    19501 ggcgaacacc agaccaacta cagataaagt aaaagaatcc atattcaata tgattggacc
    19561 atattttgat ggagagacag tattggatct gtttgcagga agcggtggtt tagctatcga
    19621 agcagtatct agaggctgca gtcatgcggt atgcgtagac aaaaattacc aagcgctaaa
    19681 aatcatcaaa gaaaacatcg aaattacgaa agaaccagag aaatttacca caataaaact
    19741 tgatgcggac aaagcaatcg aacggctagc agcggagaaa caacaattcg actacttgtt
    19801 tcttgatccg ccatatgcaa aacaaaaaat aatcgatcaa atccataaaa tggaagaact
    19861 gggacttttt tctaaaaccg cagttatcgt atgcgaaacg gataaaacag tcgaattgcc
    19921 agaaactgtg gctggattcc atcaaataag aaaacagatt tatggcataa cggccgtaac
    19981 tatctataga aaagagggaa atcaatgaaa cgtgcattat tcccaggaag ttttgatcct
    20041 tttacaaaag gtcacttaga tacggtggaa cgtgcggcta aattattcga tgaggtagta
    20101 attggtgttt ttatcaatac ttcaaagaaa agtttgtttc ccccagaaga aagaatgaca
    20161 ttgatcacaa aagctgtttc tcatcttcct aatgtaaaag tcatgcatca agaaaatcaa
    20221 ttgacggtag aaacagcaaa agagattggt gcagatgcgt tgatcagagg catacgaagt
    20281 atcaaagact ttgaatatga acgagagatt gctcagatga atcatcatct atatgatgaa
    20341 ttggaaactg tcttcttatt agcaaaacca gaatatagtc acgtgagttc cagtatttta
    20401 aaagaagtac tgcactttgg cggagatgtg agcagctact taccgccagt gatcaacgaa
    20461 gctttagctg gaaagcgtga atcaaatgaa agctaaacaa tggttgaaaa cagtactgat
    20521 tatcatattt ggaatgttga tcattggatt tgtagtagtt cccttgccgt attatgtaga
    20581 atcaccggga gccacgatcg atctgaaaga actaattacg gtcaatgata aggaagataa
    20641 aggttctggg tctttctctc ttacctctgt tggtatcagg caagctacag gtttcttagc
    20701 tttaaaagct aaaatgaccc cttttgaaga attgatcagt gaagaagaat tgactggtgg
    20761 ggcgacaaat gaagaatata atcaaatgca acagtattat atggattctt cccaaaatgc
    20821 agcaatcgaa caagcgttaa aacttgcaga caagccttat aaaatggaat tcaaaggcgt
    20881 atatgtcatg agtgtagaga aaacatcgaa tttctttggc aagctttcag ttggagatac
    20941 agtaacaaaa gtagacggga aaagctttca aagtacagaa gcatttatgg attatgtaaa
    21001 aagccaaaaa gttggacaaa caatcgaaat cagttatttg cataatggtg aagaaaaaac
    21061 agcttctggt gatctgatcg agttaccaac agataaaaag gcaggaatag gtatcacgct
    21121 tactgaccat accgagatcg aatcagatac agatgttcgt attaattctg gctctatcgg
    21181 tgggccatca gcgggattga tgttcacttt agaaatctat gaacaagtca ctggtaaaaa
    21241 cttgcgacat ggtaagcaaa ttgcaggaac tggaacgatc aacagtgaag gagaagttgg
    21301 ccgaatcgga ggaatcgata aaaaagtggc aagtgcggat aaagctggcg tagagatttt
    21361 ctttgcacca gatgatacga tttcagacga tgttaagaaa gaatatcctg aaataaaaac
    21421 caactacgaa gaagcaaaag ctgccgctaa aaaattgaat tcctcaatga aaatcgtacc
    21481 agtaaaaaca gtacaagaag cgcttgacta tctagagaaa atgaaatgaa actaaataat
    21541 taaagagggg ttgtgacacg agttctgtca caacccctct ttttcgtc
//
LOCUS       DASHFF010000044        21280 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.044, whole genome shotgun sequence.
ACCESSION   DASHFF010000044 DASHFF010000000
VERSION     DASHFF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.044"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            132..506
                     /locus_tag="U2I75_001843"
     CDS             132..506
                     /locus_tag="U2I75_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1696968.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="U2I75_001844"
     CDS             complement(620..1249)
                     /locus_tag="U2I75_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1696969.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="U2I75_001845"
     tRNA            complement(1383..1455)
                     /locus_tag="U2I75_001845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
     gene            1669..1742
                     /locus_tag="U2I75_001846"
     tRNA            1669..1742
                     /locus_tag="U2I75_001846"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1703..1705,aa:Asn,seq:gtt)
     gene            complement(1880..2044)
                     /locus_tag="U2I75_001847"
     CDS             complement(1880..2044)
                     /locus_tag="U2I75_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696970.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2339..3667
                     /locus_tag="U2I75_001848"
     CDS             2339..3667
                     /locus_tag="U2I75_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN1696971.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3869..4702
                     /locus_tag="U2I75_001849"
     CDS             3869..4702
                     /locus_tag="U2I75_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN1696972.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4718..5455
                     /locus_tag="U2I75_001850"
     CDS             4718..5455
                     /locus_tag="U2I75_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN1696973.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5610..6578)
                     /locus_tag="U2I75_001851"
     CDS             complement(5610..6578)
                     /locus_tag="U2I75_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN1696974.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="U2I75_001852"
     CDS             6810..7643
                     /locus_tag="U2I75_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN1696975.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="U2I75_001853"
     CDS             7677..8516
                     /locus_tag="U2I75_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1696976.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="U2I75_001854"
     CDS             8542..9102
                     /locus_tag="U2I75_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1696977.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="U2I75_001855"
     CDS             9301..11022
                     /locus_tag="U2I75_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN1696978.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11187..12329
                     /locus_tag="U2I75_001856"
     CDS             11187..12329
                     /locus_tag="U2I75_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1696979.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12345..13556
                     /gene="thiI"
                     /locus_tag="U2I75_001857"
     CDS             12345..13556
                     /gene="thiI"
                     /locus_tag="U2I75_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN1696980.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14129..14776
                     /locus_tag="U2I75_001858"
     CDS             14129..14776
                     /locus_tag="U2I75_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1696981.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14905..15121
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15169..17814
                     /locus_tag="U2I75_001859"
     CDS             15169..17814
                     /locus_tag="U2I75_001859"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN1696982.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18124..19437
                     /locus_tag="U2I75_001860"
     CDS             18124..19437
                     /locus_tag="U2I75_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN1696983.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19427..20080
                     /locus_tag="U2I75_001861"
     CDS             19427..20080
                     /locus_tag="U2I75_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1696984.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20135..20827
                     /gene="radC"
                     /locus_tag="U2I75_001862"
     CDS             20135..20827
                     /gene="radC"
                     /locus_tag="U2I75_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEN1696985.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20977..21177
                     /locus_tag="U2I75_001863"
     CDS             20977..21177
                     /locus_tag="U2I75_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1696986.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         7183 a         3551 c         4445 g         6101 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tacttgattc cggcatagct
     1681 cagttggtag tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc ctactgccgg
     1741 agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct ttttctttta
     1801 ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt tacgaaagag
     1861 tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg aaataaatca
     1921 gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca ttcccaaagt
     1981 tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca atcaggtttt
     2041 tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg agtattttga
     2101 aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag gccattttcg
     2161 ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat gacatttttc
     2221 ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga agagattgta
     2281 tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg atgattggat
     2341 gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag caaatatcaa
     2401 acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg ctgaaaatgt
     2461 ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg ggaaagtggc
     2521 taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat tccgtcataa
     2581 gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca cttttttctg
     2641 ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag gaaaagaggc
     2701 aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg gcggccaatg
     2761 ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg tgatgccgga
     2821 aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat tactgcgtaa
     2881 agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg ctattgaaga
     2941 aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat taggaacacc
     3001 tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc ttgaccgagg
     3061 actgacacca caaatttttt atgataaata catcggtgta aaattagatg attatgttag
     3121 cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag tagagatgct
     3181 aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg aaactttcaa
     3241 aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt gtgatgtagg
     3301 tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa tggatgatct
     3361 atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag aaagtctgat
     3421 gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa caaaatggaa
     3481 agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta tgagtgatgc
     3541 ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga cgaaagagca
     3601 attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa tgggtgcttt
     3661 agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc tggcttaagc
     3721 cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg ctataattct
     3781 aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat gacgaagtag
     3841 aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat ctgatctgaa
     3901 attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct atcgtataaa
     3961 aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag gattttttga
     4021 agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg atacgtatat
     4081 gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc cgggaaaagg
     4141 ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag cgccacaatt
     4201 tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt ttgaagaaga
     4261 ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg cagaagaaac
     4321 gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac gtgtattgaa
     4381 atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt ggagtggtaa
     4441 tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt cttatgggaa
     4501 tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg aatttttaga
     4561 tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc cgatttacca
     4621 gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt cccaagtaga
     4681 tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat ataccaaaaa
     4741 gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc cttcgatgtt
     4801 aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag gcagtgaatt
     4861 cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga ttaatcgtct
     4921 ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca ataagttttt
     4981 ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca aaatagaatc
     5041 tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg aagagatcat
     5101 tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa ttgaaaagat
     5161 tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata gtaatcagca
     5221 tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg actttttgac
     5281 tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt acgggaatga
     5341 gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat
     5401 ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata aataaaaaag
     5461 aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct
     5521 caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct
     5581 ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca
     5641 cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg actattaata
     5701 ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa
     5761 acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca
     5821 agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg
     5881 attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca
     5941 gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt
     6001 tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat
     6061 gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa
     6121 tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg
     6181 acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc
     6241 attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca
     6301 gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa
     6361 gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga
     6421 agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct
     6481 aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg
     6541 atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
    11041 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
    11101 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
    11161 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
    11221 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
    11281 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
    11341 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
    11401 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
    11461 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
    11521 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
    11581 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
    11641 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
    11701 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    11761 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    11821 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    11881 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    11941 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    12001 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    12061 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    12121 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    12181 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    12241 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    12301 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    12361 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    12421 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    12481 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    12541 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    12601 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    12661 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    12721 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    12781 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    12841 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    12901 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    12961 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    13021 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    13081 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    13141 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    13201 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    13261 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
    13321 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    13381 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    13441 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    13501 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    13561 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    13621 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    13681 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    13741 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    13801 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    13861 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttgcg
    13921 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt
    13981 acgtttggtt gagcttatta ccgcagatgc aagcttttga acaccgtttt atttgtctca
    14041 cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt acactaaata
    14101 tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc
    14161 aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg actcaggtat
    14221 aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg caacgatccg
    14281 cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg atgtcccttt
    14341 tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa tcgcattagt
    14401 aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa atgaaaatct
    14461 aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa ttgatggaga
    14521 aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc
    14581 aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg tcaaaggaaa
    14641 tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt atgtcaatgt
    14701 tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg agaattactc
    14761 agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa
    14821 ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac ttgttgacta
    14881 aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa ctggcacatt
    14941 cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt gaaggtagct
    15001 tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta actcacttga
    15061 gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga
    15121 atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat gacggaagaa
    15181 aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta tcagcattgg
    15241 ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta ttcaatcgta
    15301 atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga tacgaccttg
    15361 caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg gctgcctggt
    15421 atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag agagcaaggg
    15481 atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa
    15541 gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt agattacagt
    15601 agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt ttttgttacc
    15661 ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga tcctcaagca
    15721 cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc cttttatcat
    15781 atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa
    15841 acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta tcaagcgctc
    15901 atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat tgcagatgaa
    15961 tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca tgatccaaac
    16021 gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga tgatgatggt
    16081 acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc tcgtaaagca
    16141 atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat gatccacagt
    16201 gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt
    16261 gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga tcaagcagtg
    16321 gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa tgtccatgat
    16381 tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta tcacaaagaa
    16441 acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg ggtacaagat
    16501 cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac aatgggctgg
    16561 ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt agtaaccggt
    16621 tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga atttactggc
    16681 gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca agggcggaag
    16741 atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa gtatggagca
    16801 gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt ccgcttcagc
    16861 tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc
    16921 gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg agaaaaatca
    16981 gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt aactgagcta
    17041 tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat ctgggatgat
    17101 ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga tgctgtttca
    17161 aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg tttattgcat
    17221 ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca gggaaattct
    17281 ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac agcttctaaa
    17341 gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc agaagtgaat
    17401 acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa aatcgatcaa
    17461 tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga gttgacgata
    17521 tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg tgcagaaatc
    17581 ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa
    17641 ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt
    17701 gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa agattattta
    17761 gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca ataagaatag
    17821 aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga acatcgttac
    17881 aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga cataaatttt
    17941 gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg tggatcatgt
    18001 atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt ttctctttgt
    18061 catactttta tttttcttat gtgttctatt tgttataata aaagagaaat gggggaattg
    18121 ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt tggttcacgt
    18181 cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga gagaaaagta
    18241 aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta tttgagaagc
    18301 ctattagaag aggctgggct gacagtaggt acatttactt caccttatat cgaaacgttc
    18361 aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat ccagcttgta
    18421 aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc tggtattact
    18481 gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa agtggacgta
    18541 gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc tgtaccaatg
    18601 ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga tacaattgaa
    18661 gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt aacaggaaat
    18721 attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt
    18781 tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat gtggggagaa
    18841 gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc gcttttaggg
    18901 caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta ctgtcaattg
    18961 gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca
    19021 ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc ccataatacg
    19081 catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac
    19141 attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg attgcagcaa
    19201 gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat cgaattaagt
    19261 gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca attcggatta
    19321 gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg ttcactttat
    19381 ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga agtataatgc
    19441 agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt acgaagccac
    19501 acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt taaaattctt
    19561 aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg aatttggcaa
    19621 aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac gtttttcgca
    19681 aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag aagaacatca
    19741 gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt tattgaagaa
    19801 gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa
    19861 accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga agaaagaaac
    19921 attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg agatcccggt
    19981 agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa cagctggggt
    20041 tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct cttttcgtat
    20101 cttattttag ataagataga aaggagatgt ttttttggga aaacgattga tcaaagaagt
    20161 acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag ctttatcgga
    20221 tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg tgatgtcgat
    20281 tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg ctactttgca
    20341 tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca aagccttgat
    20401 cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg ttcgtaccag
    20461 ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag aacatttggt
    20521 ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc
    20581 gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac gttattgtgc
    20641 agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat cgcagcagga
    20701 tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca ctgtactgga
    20761 ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa tgatggaaga
    20821 gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat tgtaattttg
    20881 tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca atgttacaat
    20941 ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac agtaaaatgg
    21001 ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga cgtattcgtt
    21061 cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca agcagtgact
    21121 ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa agcttaattt
    21181 tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa gcgagtaaca
    21241 aaaatacctg ccacaagaga caatttctct tgtggcaggt
//
LOCUS       DASHFF010000045        21247 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.045, whole genome shotgun sequence.
ACCESSION   DASHFF010000045 DASHFF010000000
VERSION     DASHFF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.045"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(137..514)
                     /locus_tag="U2I75_001864"
     CDS             complement(137..514)
                     /locus_tag="U2I75_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1696987.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            770..1498
                     /locus_tag="U2I75_001865"
     CDS             770..1498
                     /locus_tag="U2I75_001865"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN1696988.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1498..1752
                     /gene="purS"
                     /locus_tag="U2I75_001866"
     CDS             1498..1752
                     /gene="purS"
                     /locus_tag="U2I75_001866"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN1696989.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1754..2425
                     /gene="purQ"
                     /locus_tag="U2I75_001867"
     CDS             1754..2425
                     /gene="purQ"
                     /locus_tag="U2I75_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN1696990.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2426..4648
                     /gene="purL"
                     /locus_tag="U2I75_001868"
     CDS             2426..4648
                     /gene="purL"
                     /locus_tag="U2I75_001868"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN1696991.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4633..6072
                     /locus_tag="U2I75_001869"
     CDS             4633..6072
                     /locus_tag="U2I75_001869"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN1696992.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6095..7147
                     /locus_tag="U2I75_001870"
     CDS             6095..7147
                     /locus_tag="U2I75_001870"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN1696993.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7144..7722
                     /gene="purN"
                     /locus_tag="U2I75_001871"
     CDS             7144..7722
                     /gene="purN"
                     /locus_tag="U2I75_001871"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN1696994.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7719..9254
                     /gene="purH"
                     /locus_tag="U2I75_001872"
     CDS             7719..9254
                     /gene="purH"
                     /locus_tag="U2I75_001872"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN1696995.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9270..10544
                     /gene="purD"
                     /locus_tag="U2I75_001873"
     CDS             9270..10544
                     /gene="purD"
                     /locus_tag="U2I75_001873"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN1696996.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10666..11580
                     /locus_tag="U2I75_001874"
     CDS             10666..11580
                     /locus_tag="U2I75_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1696997.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11713..13278)
                     /locus_tag="U2I75_001875"
     CDS             complement(11713..13278)
                     /locus_tag="U2I75_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1696998.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13271..14722)
                     /locus_tag="U2I75_001876"
     CDS             complement(13271..14722)
                     /locus_tag="U2I75_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1696999.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14736..15752)
                     /locus_tag="U2I75_001877"
     CDS             complement(14736..15752)
                     /locus_tag="U2I75_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1697000.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTSDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15972..17060
                     /locus_tag="U2I75_001878"
     CDS             15972..17060
                     /locus_tag="U2I75_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN1697001.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17114..18640
                     /locus_tag="U2I75_001879"
     CDS             17114..18640
                     /locus_tag="U2I75_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN1697002.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18642..19847
                     /locus_tag="U2I75_001880"
     CDS             18642..19847
                     /locus_tag="U2I75_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1697003.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20104..21081
                     /gene="guaC"
                     /locus_tag="U2I75_001881"
     CDS             20104..21081
                     /gene="guaC"
                     /locus_tag="U2I75_001881"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN1697004.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         6813 a         3596 c         4822 g         6016 t
ORIGIN      
        1 ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta ttatattttc
       61 ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc ctctttacat
      121 acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa tgctggttat
      181 ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca ttgaataaga
      241 tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg attgaaagtt
      301 cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca cagcgcttgg
      361 ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat aaataaacgt
      421 ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt aattctgata
      481 aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc gaacccatta
      541 tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa cgcttgttga
      601 tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa tgccttaaat
      661 gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa
      721 caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca tggagaagaa
      781 agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg agaacttgat
      841 ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag accagataag
      901 tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc tgactcaaag
      961 aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt
     1021 acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt tctcaaaacg
     1081 tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct actataagga
     1141 tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac aacttgcaaa
     1201 gccggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa ttttgaccga
     1261 attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg gccggtcgaa
     1321 tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat gggatgaacg
     1381 tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt tgatcccagt
     1441 ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc gagtatgatg
     1501 tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca aggggaggca
     1561 gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg aataggaaaa
     1621 tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt
     1681 tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg
     1741 gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga
     1801 tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac ggcatgatga
     1861 gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct atggtgacta
     1921 tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga ttcgttttgc
     1981 tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga cagaagcagg
     2041 acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca aaactgtacc
     2101 attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg aaataatcta
     2161 tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac ttcaagaact
     2221 aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca gcgtggaaaa
     2281 cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc atccagaacg
     2341 tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt ccattttaaa
     2401 aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag ctgaagagat
     2461 caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc gaatgatcga
     2521 agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt ctgttatgtg
     2581 gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc ctactagtgg
     2641 acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg gtgatggact
     2701 agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc cttatgaagg
     2761 agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg ctcgtccaat
     2821 tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga aatatttact
     2881 agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac cgactgtagg
     2941 cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg caatgtgcgt
     3001 aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg gtaattcgat
     3061 catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt ttgcctcaga
     3121 agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg atccttttat
     3181 ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg attctctaat
     3241 aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa tggcttcaaa
     3301 agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa ctgggatgac
     3361 accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg ttaaatcagg
     3421 agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag tcactatcgg
     3481 taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag tagcaaatct
     3541 gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga aagaaccagc
     3601 tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag aacctggaca
     3661 agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa tctacgaaac
     3721 ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat
     3781 gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg cgcgctatct
     3841 gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta gaaatattat
     3901 tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt ctcctgataa
     3961 accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg cgtgtgaaac
     4021 gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg atggtcaagc
     4081 aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata aacatatcac
     4141 tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga cgtttgcaga
     4201 ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa
     4261 ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca tccaaaacgg
     4321 attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac tggcagaatc
     4381 agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg ctcaattatt
     4441 tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga cacgattcga
     4501 agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag cgataatcga
     4561 aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa agtgctggga
     4621 ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt
     4681 gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag tcttcagcat
     4741 cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga
     4801 gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca gttaacagga
     4861 gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt ggacaatatt
     4921 cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa tggtaacttg
     4981 atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt ccattctaac
     5041 tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga ttttttaaat
     5101 aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt aatgacagaa
     5161 acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat cggacaaatg
     5221 aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat tggggcacgt
     5281 tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg aatccagatt
     5341 gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat ctattttgcc
     5401 agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa tatgggacgg
     5461 aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc taattcttct
     5521 ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat cggattagta
     5581 aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag agagcaaggt
     5641 gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt gattatggtc
     5701 gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt gaaagaagca
     5761 ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc atgtttttac
     5821 gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat tgatgaaatc
     5881 aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct gatcgaatcg
     5941 atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt caacggagat
     6001 tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa agaaaaagta
     6061 tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa atgcatacgc
     6121 aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca agaagcatgt
     6181 caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg gttgttttga
     6241 tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg gcgtggggac
     6301 gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg actgcgtagc
     6361 gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct tagactactt
     6421 agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg
     6481 atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc ctggtatgta
     6541 ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa aatctcaatt
     6601 attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat ctagcgggat
     6661 tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc agttttcttt
     6721 atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa
     6781 aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag gagctgcaca
     6841 tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat ctatggctgc
     6901 acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac aaaaatatgg
     6961 tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta tggtcctggc
     7021 agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt
     7081 ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta taggagaaga
     7141 acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc gctagccgat
     7201 tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga tcagcctgaa
     7261 gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt ttcacctaaa
     7321 gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag
     7381 atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt gctgaaaaat
     7441 tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc tggtttgcat
     7501 ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat tcattatatc
     7561 gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat tgaacaggag
     7621 gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat ctatccagaa
     7681 gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca ttgatcagtg
     7741 tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa ggagtagaaa
     7801 tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa acgctttcga
     7861 tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca cttcatccta
     7921 aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag gcaatggaaa
     7981 cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc aaagaaacga
     8041 ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc ggtggtccta
     8101 gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt gatccatctg
     8161 actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt tctttcagac
     8221 aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg atcgctcaat
     8281 acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa
     8341 aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat cagtccgctc
     8401 ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa ctttcttata
     8461 ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac gagccaacgg
     8521 tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg attttagaag
     8581 cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc atcgttttga
     8641 atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta gaaatcatca
     8701 ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa aacatccgat
     8761 tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg gtctcagtct
     8821 taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa gagtggaaag
     8881 tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt gcttggagag
     8941 ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa
     9001 tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag
     9061 agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat gattcggttg
     9121 aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc atcaaagacc
     9181 aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact gatgttcggc
     9241 attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc
     9301 gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg gtgtattgtg
     9361 cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc agtgagcgag
     9421 atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt atcgttggtc
     9481 ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga ttgaaaatct
     9541 tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct aaacaattaa
     9601 tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac gaaaaagcag
     9661 cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg
     9721 ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca gaacaaatgt
     9781 tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag tttttggcag
     9841 gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt
     9901 cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc ggaatgggtg
     9961 cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg gaagaagttt
    10021 taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt gttttatatg
    10081 cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg
    10141 atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg
    10201 atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac aatgtaggcg
    10261 ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt ctaccagatt
    10321 ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac ggcaaactag
    10381 ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc aaagaagcac
    10441 agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt tatcgtacag
    10501 atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt
    10561 tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct tttcataaag
    10621 agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa aaaaattcaa
    10681 ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg tatcaacaat
    10741 gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga tttttttgac
    10801 catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa
    10861 acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt tccaggtgtg
    10921 atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt gacccgtctg
    10981 aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt agaaccagag
    11041 gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc
    11101 agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca accattagta
    11161 gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg gattcatgtc
    11221 aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta ttcccgattg
    11281 catgatatga cgatccaagc atggtcacca taccagtatg gattttttga aggtgtattt
    11341 atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc agctaaatat
    11401 caaacgacac caactggctt ggcttctgct tggattttac gccatccagc caatatgcaa
    11461 gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt
    11521 aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat tttaccttaa
    11581 agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa cttgaaagct
    11641 gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt atcattgtac
    11701 ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat attcttttgg
    11761 agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat tataaccaat
    11821 ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt ttgctaattc
    11881 gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt
    11941 actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg acatatctgg
    12001 atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct gatatttgtt
    12061 ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg
    12121 gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc aatgaaccat
    12181 ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag tcaagacaaa
    12241 taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta
    12301 cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa agtaaaagac
    12361 agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa tttgttcttt
    12421 aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag gtaattcgct
    12481 gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc ccttttctaa
    12541 ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt cttcaagaga
    12601 atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca cttgttgatt
    12661 ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa agtctgtact
    12721 gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc gattgaaaga
    12781 ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa
    12841 atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa
    12901 atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt taatcggttt
    12961 aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga aatcgtcgaa
    13021 tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt
    13081 caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct gtaagtcgat
    13141 aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga ttggataata
    13201 agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt cgtcttcgat
    13261 gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga gggatcatca
    13321 gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt tgatcatttc
    13381 cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg
    13441 aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca gggatgccaa
    13501 tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgtg gtaatacgaa
    13561 taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca atcaatggct
    13621 gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg atcttatagt
    13681 caagcatatg gccgtatctt atttttaaga tgactagata atcattgata tggctaattt
    13741 ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa
    13801 gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg
    13861 catccaaact gttataaata aaatggggag taatctgagc ttgcaataca cgaacttcac
    13921 tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt agatcttccg
    13981 ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc agggttaaac
    14041 gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga ttgatcggtt
    14101 cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa atggtactaa
    14161 gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc gtattttttt
    14221 tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac agcagttgag
    14281 tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct tttgccaaga
    14341 tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt agcgatacat
    14401 ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga atatcattcc
    14461 taagttcttc taggatattt tcctcattga actcatctac ggttacttgg ccagaaatca
    14521 agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca tctgcttttt
    14581 ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac agtatgctca
    14641 gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca aaaatctgtt
    14701 ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt tgggatgtta
    14761 gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg taatgttttt
    14821 cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca gccgacatca
    14881 ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat catatcttct
    14941 tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt ttcttttatc
    15001 gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc gatttcggat
    15061 aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg aataaaatca
    15121 ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc atcttttttt
    15181 agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg
    15241 gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga atttgtgaag
    15301 tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg gccaatgtgg
    15361 gtgacgaaaa gctcgggatc actcgtttga atatgtctgt cgacaagtat gacagggatc
    15421 cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa aggagtaaaa
    15481 atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg ccgttcctga
    15541 ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc agtgataatt
    15601 gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa accaacgact
    15661 agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta agcgattaca
    15721 acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg gatttgttag
    15781 tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc
    15841 aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa agtaaatccc
    15901 ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc aattgaaatg
    15961 ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct attttgaccg
    16021 caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt acggggtatg
    16081 taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg aacaacatgg
    16141 tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa gataaagtag
    16201 aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca cttgtgattg
    16261 cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa gcagatatca
    16321 aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat tacgcgactt
    16381 ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac ttaggattga
    16441 aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac gacaacaacg
    16501 cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac aacggtcagt
    16561 tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt
    16621 ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac caaacattgg
    16681 atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca ttaaaaggcg
    16741 tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat gcgacagttg
    16801 ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa gacacacgta
    16861 tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa gaggttccgg
    16921 tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat ttagcaaata
    16981 cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat tattataaag
    17041 aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct aagtcagaga
    17101 gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca aaaaattttc
    17161 tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt
    17221 gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc
    17281 atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat ttaaagattt
    17341 gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac taagtcctta
    17401 tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag gcgtgattga
    17461 ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt tgtctatcga
    17521 cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg aaatagctaa
    17581 agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg cattgaatga
    17641 agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag gtgtaacctc
    17701 catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt
    17761 acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag agcatattat
    17821 tcgcggaatg gtgggcagag atttagttga tcgttatcct gaaagaaaac cggatattgg
    17881 agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc acgagcgaat
    17941 cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa tagctggact
    18001 gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt atggaagcaa
    18061 gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg ttccttcagc
    18121 cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac tgaatctgct
    18181 gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc aaaaaggagt
    18241 attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga tgcgtataaa
    18301 aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt
    18361 tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc caacccgtgg
    18421 gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg cagcaaatgg
    18481 caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat
    18541 ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga
    18601 agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc
    18661 ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc
    18721 gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg
    18781 gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc catatattag
    18841 tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt
    18901 cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc
    18961 cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt
    19021 tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt
    19081 ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt
    19141 tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct
    19201 tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa
    19261 gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg
    19321 cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt
    19381 atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata
    19441 aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat
    19501 cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag
    19561 ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat
    19621 cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca
    19681 ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca
    19741 tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt
    19801 tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt
    19861 attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac
    19921 attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa
    19981 ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa
    20041 aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat
    20101 gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat
    20161 agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta
    20221 gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat
    20281 ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa
    20341 atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc
    20401 tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg
    20461 catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa
    20521 acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg
    20581 ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag
    20641 acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct
    20701 cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc
    20761 cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct
    20821 ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa
    20881 ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga
    20941 tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct
    21001 ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt
    21061 tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga aaaagttgtc
    21121 ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt tgtttggctg
    21181 gaattattat cgcagataca agcttttgaa catcattatt cagaataagg gactgtgata
    21241 agatttt
//
LOCUS       DASHFF010000046        21226 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.046, whole genome shotgun sequence.
ACCESSION   DASHFF010000046 DASHFF010000000
VERSION     DASHFF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.046"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(578..1465)
                     /locus_tag="U2I75_001882"
     CDS             complement(578..1465)
                     /locus_tag="U2I75_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1697005.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            1650..2489
                     /locus_tag="U2I75_001883"
     CDS             1650..2489
                     /locus_tag="U2I75_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1697006.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2685..3659
                     /gene="sgrA"
                     /locus_tag="U2I75_001884"
     CDS             2685..3659
                     /gene="sgrA"
                     /locus_tag="U2I75_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEN1697007.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            3957..4313
                     /locus_tag="U2I75_001885"
     CDS             3957..4313
                     /locus_tag="U2I75_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697008.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            4316..5473
                     /locus_tag="U2I75_001886"
     CDS             4316..5473
                     /locus_tag="U2I75_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697009.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5499..5939)
                     /locus_tag="U2I75_001887"
     CDS             complement(5499..5939)
                     /locus_tag="U2I75_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1697010.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            6099..6605
                     /locus_tag="U2I75_001888"
     CDS             6099..6605
                     /locus_tag="U2I75_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1697011.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6773..8685
                     /locus_tag="U2I75_001889"
                     /pseudo
     CDS             6773..8685
                     /locus_tag="U2I75_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            8690..9691
                     /locus_tag="U2I75_001890"
     CDS             8690..9691
                     /locus_tag="U2I75_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1697012.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(9893..10099)
                     /locus_tag="U2I75_001891"
     CDS             complement(9893..10099)
                     /locus_tag="U2I75_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEN1697013.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(10745..11593)
                     /locus_tag="U2I75_001892"
     CDS             complement(10745..11593)
                     /locus_tag="U2I75_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1697014.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            11592..12659
                     /gene="gnd"
                     /locus_tag="U2I75_001893"
     CDS             11592..12659
                     /gene="gnd"
                     /locus_tag="U2I75_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEN1697015.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            12661..13662
                     /locus_tag="U2I75_001894"
     CDS             12661..13662
                     /locus_tag="U2I75_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEN1697016.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            13659..13943
                     /locus_tag="U2I75_001895"
     CDS             13659..13943
                     /locus_tag="U2I75_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEN1697017.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            13976..14773
                     /locus_tag="U2I75_001896"
     CDS             13976..14773
                     /locus_tag="U2I75_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1697018.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            14787..15620
                     /locus_tag="U2I75_001897"
     CDS             14787..15620
                     /locus_tag="U2I75_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1697019.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            15638..16495
                     /locus_tag="U2I75_001898"
     CDS             15638..16495
                     /locus_tag="U2I75_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1697020.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            16517..17026
                     /locus_tag="U2I75_001899"
     CDS             16517..17026
                     /locus_tag="U2I75_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1697021.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            17063..17464
                     /locus_tag="U2I75_001900"
     CDS             17063..17464
                     /locus_tag="U2I75_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697022.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            17576..17929
                     /locus_tag="U2I75_001901"
     CDS             17576..17929
                     /locus_tag="U2I75_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697023.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            17963..18406
                     /locus_tag="U2I75_001902"
     CDS             17963..18406
                     /locus_tag="U2I75_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1697024.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            18423..19508
                     /gene="uxuA"
                     /locus_tag="U2I75_001903"
     CDS             18423..19508
                     /gene="uxuA"
                     /locus_tag="U2I75_001903"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1697025.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            19501..20214
                     /locus_tag="U2I75_001904"
     CDS             19501..20214
                     /locus_tag="U2I75_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1697026.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(20421..20570)
                     /locus_tag="U2I75_001905"
     CDS             complement(20421..20570)
                     /locus_tag="U2I75_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697027.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            20966..21166
                     /locus_tag="U2I75_001906"
     CDS             20966..21166
                     /locus_tag="U2I75_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1697028.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7264 a         3272 c         4390 g         6300 t
ORIGIN      
        1 aataaacgat agccttctcg aaaactcgag gaggctatct gacgattcaa tattcattaa
       61 tttaaagttt gggtgactat tactaactat tacttatttc tcatttaaac gatgtattcc
      121 ctaacctcta atacatcctt gtaactctga agttacgcca gttttgaata aatttcataa
      181 aaacaaatga aaagtgtcgc ataaaaatat ctttcgtgtt tctctagtat aacagaataa
      241 gaagaacaag tccagaaggg atatttttag tcttttttaa aataactttt cagtcgagtt
      301 tgagtttatg atagttgttt ttgaagcgat acggcaagtg aaaaaatagg gaagcatgaa
      361 agaatctact taatggcgca ttgattcatg tgatagaaat aaaaaacttt tttaaattat
      421 atcctcatga ttgtaacaaa tccagtgatt tgttttggta caaataaaaa aaggccccga
      481 tgaagttgtg gcaaaagtgg tttgcctcaa aaataagaag gaatttccaa aaatcacttc
      541 tcaaattttg ccacatcccc aattgggtca aatttgttta tttgaatata ttttttacat
      601 aaggatcttt aatagttaca ttgtcttttt tcatcacttt acggattgtt ttatccatga
      661 attccgtatc aagagttttt gcttcggtag caattttttc tagctcagat ttgtatttgt
      721 ctttattaga acctttatcc tgttttttta ccatttttac tagataatag ctttgctgat
      781 aagtcgttgg atctgttaca ggaatgatat cagaaacttc cccgtttttt agtttaaatg
      841 ctgctttttt tacttcagta ggaatctctg gattggttga atcaaaattc atttttccat
      901 cagtttcctt taatgtttta ttttttccat aagcttgaac aagcttggag aaattttctc
      961 cttcatttgc ttcttttttt gctttatctg cttcttcttt agtcgaaaat agaatgatct
     1021 gtgtactaac ttctggatag aattctttcc aagcagtgtc tagatcttta tctgttaatt
     1081 ttacattggc tttcaaaccg tattggaaag ccagcatctg tttaaagagt ttcttatatg
     1141 tctcttctgt ttggccaact gcttttaatt gttccttgta gtttggaccg agttttttga
     1201 tttgttcatt atattctttt gttacttcct ttgaagaaat ctcttttccg tatacatcat
     1261 caaatgcttt gaagacaatc atttttttta atagtgtttg attatttgat ttaacttttg
     1321 ggttatcata aaaatcctga actgtaagtg ttccaccttt aaacgttgca atcgtttcat
     1381 tggacttgct acaacctgag aatagtaaaa ttgcacatgc tgaaaggcac ataattagta
     1441 gttgtttttt caaaaaattt cccatccttt ccttattaaa aacagtctaa cagaagaatt
     1501 agggagacag tctttcattt tcgtaaggtt gctgtagata ttcttagttc gtaaaaatct
     1561 attctctcta tgatggagaa catttacata gttttcacga gacaagaaag aaaagggttg
     1621 ttaaacttaa tataaaggag ggatgaaaga tgatcaggat tattcggttg attggatctg
     1681 ttttaatagt cttagttggt tttgttttag gaattcttgt caataatgca cttgcggata
     1741 tgtttattgg aaatagtgaa tggattggag caattgccgg tacggtcgga catttagctg
     1801 tttttttact agctttattt cttgctagta aggtcgaagg aaaaagaata gaagattatg
     1861 gtgtttctgt tcgaggaaaa gactgggggt atttagcggg aggtctcgtt gttggaattg
     1921 gagtgtttct gcttattact agtccattat atttgacggg ggcgtatcgg ttagattcag
     1981 gaaacgcaaa cattgtgccg ctgataacga gttttatttt atttattgca gtgggggcat
     2041 cagaagagct tttattccgt ggcttttttc agcatcagat gcttagattt ggtccgttaa
     2101 tagcaatgat tggatctgct gcattatttg ctcttttgca cggattgaat cctaacatga
     2161 catttctggc agttttcaat atctttttag caggttgctt tttcagcgca cttatctaca
     2221 gtacaggaag tttatttacg gctattggtg ctcatattac ttggaattgg acacaaggag
     2281 ctattttggg tataccagtc agcggaacgc aagaacaagg ttttttctcc actctgatcc
     2341 aatcgcaaaa tacaataatt actgggggaa actttggggc tgaagcatct atatcaacta
     2401 cattattctt gttagtcttg attgttggtt tgttactcta tgcatataaa acaaagcgga
     2461 tgaagaaata cactgctgtt tatcaataaa agatgaagaa agcaaggctg atatcggtct
     2521 tgctttttta agtctgtcat tgattaaacg aaaaagtaaa caaattcaag cgaatacacg
     2581 acatcttttg ttagattttc acaactgact gtcgtgttct catcattttc attttatata
     2641 attcctttat caaatagaag gtaattatat ggagagtgat agaaatgaag aaaacagcga
     2701 ccgtattatt tgttattctt gggctgagta taattgcagc aatgattgga atcagtactt
     2761 ctgcaaatga acgggcaaat gagaatcttt catttactgt gaaaactgat cggattgttt
     2821 atgacatgac acaacaagtc attactattc cagtcaaacc aaacaaaagc gtgaatgcta
     2881 gtgatgtaca tgctgttcta acatatggtt gggatggtaa tggaagttca gaaaaagtta
     2941 ttggggaagt ttatttaaaa gacgtacaat ggaccgctgg tattgagtat acaataatga
     3001 tttctgcaga actatcgatt gatgagatca agagtaaaga taaagttgat ttgattgttt
     3061 tttatgatgg acagatgacg atcacggaga atttgaaacc aagcagttgg acagttgttg
     3121 gaccgccaag cagtacggat agcacaagta gtgaaagcag tacagaaaac acgagcggtg
     3181 aaagcagtac agagagtacg agcagtgaaa gtagcacaga aaacacgagc agcgaaagca
     3241 gtacggagag tacgagtagt gaaagtagca caggaagcac gagcagcgaa agcagtacgg
     3301 agagtacgag tagtgaaagt agcacaggaa gcatgagtag tgagagtagc acaggaagca
     3361 cgagcagcga aagcagcaca ggaaaagtaa ataatgaact tgatttggta cctatcatac
     3421 caactaagga acaaaagaga aatgcaggga gcaacctaaa aagtgtgcta aaacagcctg
     3481 attttgtttc tcgtggaatt gatcatcaag aaaaagcgaa agaaacagat aatagaaaaa
     3541 gcgatttacc agaaactggt agtagaacct tgaacaagct aattacatgg atgggtgtgt
     3601 tactcatttt gattgtagga gcaagctatt ttcgttctct cttccatcga gtgaaataaa
     3661 gaataaaagc aacagatcta aaattgaata aaacgataga tttttgagtg aatatttcat
     3721 tcgagagtgc tgtattgcct ggcgaagttg tagctttgcc agatctattt ttgtttacta
     3781 acatatttga gaaacaggaa ataacaggaa cacaaatgga aaataagagc aagaataatt
     3841 gacaatgcac actgcgcatg gtagtatctc tgaaaaatca gtaacgaatc tatttgtacg
     3901 ttattcatgt agctgtcgga ataagtctaa ataatagata aaaaggagta tgtactttgt
     3961 ttgaagaatt tattgatatc aatgaaaggc aagtatatca atttttgaac tattgttatg
     4021 aaagagacga gaaattatat gtggtgaagg atatcgcttt agatctaaac tacaccctgg
     4081 ctaagatgaa ttcggtgatt caacaagcag aatcgttttg cgaaaggtat ccagaatata
     4141 aattatcttt tctttcagaa aacaagatga taaaagtcga gttttcttct cagtttttac
     4201 tgagtaaagt ttattctata cttttagagg gaacgattgg ttatatttta ctggattcac
     4261 tttacaaagg tacttatcaa tccttagaaa atcttagtca aaaaattata taagtttgcg
     4321 tacgattcaa agaaaattaa gagaacttaa tactattttg aaaaactatc atttatcttg
     4381 cagcttgaaa aaaggtaatc cgatagaagg agaagaatac cgtatccgat acttttttca
     4441 cttgatgtat tggcagtcat tcgacgaaaa tcattctgat atattatcaa gtataaaaaa
     4501 taaacgtcaa ctattagaaa aatttgcgaa acatgcgcca tatacgcgta gtatagatag
     4561 aaagaaatgg atcagcctac taagaatttc gttgcaacga atgaaacaag gccactcgat
     4621 tacgtatgtc ccagaagaga tgaacaaatt tgtgcatcca ttgataacaa aagaagagtt
     4681 tacgcattac atactagagc ctttttttga agcaaacttc tggtcgatga atcacatcac
     4741 tccaacagag caagcttacc tttattttat gtttggtgta atgaacacct atttggaaga
     4801 agatttgttg gatactccag ataaagtaca agaaagttgg ggctggattg aatcgtctat
     4861 tgtagaaaat tttgaaaaaa acttcaagat ttgttttaca acttcagaaa aaaattatct
     4921 atatgtcaat ttggcaatga tccatttgta ttcaagggtt tttgggacaa aaaagaaggt
     4981 ggatgctttt ggaaaatcag cagaagacag agattttcag catatatttc cagtgatgta
     5041 tccaatcatg caacagtttt ttagtggggt agctcgcaga attccagagc ttaatactta
     5101 ttatgaaaag aacagacggt tgatttttca atactgtatg ttagtcaggg atatttttat
     5161 aaaacacgaa caacctgtca tattctatat tcaatcaaag tatggtaaaa cacaggaatt
     5221 atgggcaaag aaaaggatat tatctatgac tgaacgccca ataaaatata gtgcatccgc
     5281 tgatcagtta cctgatattg tcattagcga ttatcctatc gacaaaaagg catatgatca
     5341 aacgaataca cagatatttt actggaatgg acaacctaca aaaaatgatt gggcgcgtct
     5401 gaaagctgaa atcataaaaa tcaggaatga gaaaaataga tccatattgg aaaaattgga
     5461 tttcgttaat taagaaaagc agattgttta tgccaaaatt aagacattgc aaaaagatta
     5521 ttttctttaa aatcgacaag atgaccagaa agcaagagtt cgttgagctc ttgctttctt
     5581 ttagttttag aaaggaacat cctgatttta tcttcgctaa atccagtcaa aagttttgca
     5641 gattggtcgt agataatcgg ttttttcaat aaagaagggg atgtacgaat ctcaatatat
     5701 agttcactca aactgagttc atctattctg tgaacaagat ttttataact aatagaatga
     5761 gtagagagca tttcggtcac gccgttgttt gtcaatttta gtaagtggat aaattcattt
     5821 ttggttagtg gttgacgaat taagttctgt tcgataaacg gtatatcatg gacgataagc
     5881 caacgttttg cgcgtcttga agacgagcag ccagaacctg tatacaattt aatcatcata
     5941 cattcctcca atagctaagt atttatgaaa taataacatg aagaaatagt cgaaaagtgc
     6001 catttagagg tgtaaaataa gaaaatgttg tcacgtataa tggaactagc tagataagaa
     6061 atactattaa atcaagaaaa aagaaagaag gcacaagtat gtcaaacaca gccgacgcaa
     6121 tagtagtgat cgatttacaa aaaggggtat gttttgattc ttcgccgata cacgaattag
     6181 aaacagtgac agcattagtg aataaaagaa tcaaaacata cgaaacgatt gggaaaccag
     6241 ttattttcgt tcaacacgaa gatgctcaat tagtgccaca aacaaaacca tgggagctat
     6301 tgtcggaatt atctattcct gagcacagtt attttgtgag aaaaacccat gcgaattcat
     6361 tttataagac taatttgagt gttttattaa ctgaattgga ctgtcattcg atcgaattat
     6421 gtggggcaca agcagagtat tgtatagata cgactgtgaa gtttgctcat ggattaggat
     6481 accagcttat gatgtgtaga ggagcaacga caacttatga taatcctttt atgaaagcaa
     6541 aggaaacaat cgctttttac gagaaaattt gggataaacg atttctgaca tttttggagc
     6601 aataagttgt ttcttcgttt aaaaaaagca gaatggatac tggtttatcc attctgcttt
     6661 attacgtaaa atgagaaaat aatcctgttc tatttttatg ggataaacca tttatttttt
     6721 gtataaaaaa tgtttgcgtt tacatataat tctcctagaa acataaaaag gagtgaatag
     6781 cttgagaaca aaacaagtat taaagggagc gttggtcatc ttatttgctg taagtacaag
     6841 cgtcccgcaa tggacagtat atggagagag catttcttcc gaatcggaat ctcgggaggt
     6901 agaaaccaca gaaactactg atgctggaaa tgaaacgaaa tcgagtactt cacaaacaga
     6961 aacaaatact gaaacaacgg ttgagacatc tgttactagc acagatcaag aaacgaccgc
     7021 taccacagat aaccaacaaa cagaagaagc aacagctaca aaaatgtcaa acgacgaatc
     7081 ctcttctgct aaagtaagtg acaaagagaa tgggacagaa aatgatgata tcggtaaagg
     7141 tgaaaatttc aatggtaaag aatatttgaa ttacttcggt aaacagatga cagaaatact
     7201 taatggacca acagaagaca tcaagtcaaa attaatagag ttattagtca ctgagcttgc
     7261 tgttaatgat tatggtgtag ataaagagat tatcaaacat ctgattgcta cttatctatt
     7321 gtacgatatc gcttttgcaa aaaacagtgt gttgatgtcg atgcttggag gtacagatga
     7381 tgggacaacg cctacagaaa acacaggctt ctatccaaat acaaaacaac ttcatcaaac
     7441 tgtactaaac aaacaagatg gcaaaatgat ggatgtatat gcatattatg tcgatcaagg
     7501 ttcagataaa acagtcatga tccatggcgg atttcgaggg aattggaata acgggatcgt
     7561 tacagaagaa tacaatgatt tttataaggc cgggtataat ttattatttg ttgattccag
     7621 agcgactggt aatagcggag gcgattatgt gacctatgga caatatgagt cagatgacgt
     7681 cctttactgg ataaatcaag aagttcgaga gcggccatct caaaagattt tactttatgg
     7741 tggttcgatg ggagcagcta cgatgatgtc tgtattagcg aaagatattc ctgtcaatgt
     7801 caaaggaatt attgaaaatt gcggatttgc atcaattgat gaacaactac gttttacgta
     7861 cagccagaca gtagcccccg cactaccgga tgccataaaa aatcagttag acatcattgg
     7921 ggatcaagag catgaagatt tatttatggg ccttttgaaa caatactatt ttgatcagga
     7981 gatgcatttg gatacaaaag ctgctctacc tacgattgga atgtcagatt cattgcctaa
     8041 attgattatc catggaacag cagatgatgt agtacctgtg tctaacgcgc aaaaattata
     8101 tgagttatca ggagggtata aagatctttt attagtagaa ggagcagggc atggaaaggc
     8161 acaggaagtt gaccatgctg cctacacgaa acatgtaacg gactttttga aagtagtgtt
     8221 ccatgatcag ataaacgtaa agtatgtgga tgaaaacaat aaaagtctac tcaaagacca
     8281 ggacgaaatc cagctttacg gagcttatgg agaaaattat atgacagagc aaaagacatt
     8341 cgaaggatac gaattagcaa atgttgaagg accgactgaa gggattttta atgagacgat
     8401 accaactatt attttctatt acaaaaaaat cccagtagct ccaccaaaaa agcaagatcc
     8461 gacagagaat gcagacaata agggaaagaa tgaacctcga gaggatcaat caagtgatga
     8521 tacgcaaccg attcagatga cagtgaaagt acaaaaaaag acagatgcaa aaaaagaatg
     8581 ttgccgaaaa ctggagaaaa gaaacattcg cttctcattt attcaggtgg atttttatct
     8641 gttagcacga ttatcacatg gatttggcga agaaagagag ggtaaatgaa tgaaatggtt
     8701 caagcgaata gtgattgcag taccagttat catcgtagtt attttagtca ctgtattttt
     8761 gattaatcaa atcacaccaa agccgattag cttgcttgtt agaaaacaat ttgacacaag
     8821 tgaaaaagaa caagtatatg cccgaccaga tgatttccag caaaaaatcg aacaaatcga
     8881 tataaaaaaa gaaatatcct atccttcaaa gtacaaaaat aataatatgg atatctacac
     8941 accgaaagtg gaaaagaaaa aattaccgat tcttttctgg atgcatggag gcgcatatgt
     9001 aggaggggat aaaaatgatt gtcgtgatta tttggaatat ctatgttcag acacgcagca
     9061 aatcatcgta aatatagatt atgaacgatc accggaagca aaacatccta caccagttat
     9121 ccaattgaac gaagccattc aagcagctaa aaaagagata aaagatcaag cagattggtc
     9181 gaatatttcg attggtggaa attcggcagg tgcacaaatt agtggagaat atttgttagc
     9241 acttcaaaat gaagaaatta aaaaagctga tcatcttgat ccgactttgg agaacaagca
     9301 gattacgaag tttatttctt tgtctggtct gttggatcct tctgcactta ctcaagtaag
     9361 tgataaaatc tctagctttc tttatgaaaa atgtggatgg gcttactttg ataataagga
     9421 ctttgaacaa tcagagaagg tcaaaagttt agccttggtc agacatgcgg atcgttggac
     9481 gcaaaacaca ttcttaacgg atggaaatac aaatacgttt acgaaacaaa tggaagaagt
     9541 aacttctgta tttgagaaag cgggggtgaa agtcacaaaa gtagattatg cggagaaaaa
     9601 tgctgttttg aaccatgaat atcagtttga tttcagcaat aaacaagcgc aagaaacata
     9661 tcaaaagtta gtaatgtttt tcaaggagta aagtcttatg atgcaaaaga ggttgtaatc
     9721 tgtcacttag ctccaagtca caaggagcaa tttcaataaa caagcttttc atggtttatg
     9781 aatattgtaa cttgtgactg aggagttaga gatggcaagg ggcacctttt attcgaaatt
     9841 tgggaatatc acacgattat tgtcacgatc tcttttacgt aaattttata agtcagattt
     9901 gttgttttga tcgtttaaaa aaagcaacaa atccgaagcc tgcggaggca agaagggaca
     9961 aaaagccgag tggtaagcaa aagaatgctg gttccactaa tattccgttg ttattgatat
    10021 aaggctggga acaaagatga atagcaaata aaatcaatgc actagcgaaa aaaagcaaag
    10081 aaatatggat agctttcata agcagcacct ccttttttat aattgtatga taaatagatt
    10141 aagaatctat aaatgtgaca agtttgttag gatgtgaata aaatgatcat ctagcgaact
    10201 tgaaacaaag atggctaatg ataagcaatt aatgattcag caatcagata ggtacaaagt
    10261 ttaccatcat acgtctagac caataataga aaatatggga atataataga aaagatttag
    10321 gataccccca aaagaatttc cccaaaaatt cgatgtatcc taaacttttt tattttacat
    10381 aacaatcata tcaactgtga aataaaatta caaatgataa cctaaaatga ggccaaaagc
    10441 cagtgttatc aaggaaatac ttaattcacc aaaatttttt ttaatttttt agaaaataag
    10501 aaacaaaaat aataaaaaaa agaaaagata aattttttac tactgtatac taactttgtt
    10561 atcgcatcta acaagtaact cgaataatac caatacaacc ttctccaaag atgcgatgac
    10621 atacgttcta acccaccgac tattgatgtt ctgtaccacc cttgcagaac caaaaaagcc
    10681 actaccaaat atttggtagt ggcttttttt gttttatgga tttttcgctt tttctgttat
    10741 ggtgctattc tttgatttca gaaatagtat gtcgaacttt agcgatagaa cgacgtgatt
    10801 ctttttcgtt gtaaaacatc aaaatcacat atagagagtc caaaatgctt agttgagaaa
    10861 tacgggaagc taaagcttca gatcggaatt ctgtttcttc agaaatagaa ataaaaacga
    10921 catcgccaag tttagcaagg gaagaattag cttggctagt aataacgatt gtttttgctc
    10981 cagtttttct tacggtttca gcaatatgga ttgtttcttt tgattgccct gtgtgagaaa
    11041 tcaaaacagc acagtctttt tccgtcaata aagaagcttc catcaattga atatggtaat
    11101 ccgtgctgtg agaagttgcg ataggggaac gcaaaaattt atgataagcg tcagttgcta
    11161 caatctctga accgccaaca ccgaaaaaat agacacgttt agattcattg ataatagtag
    11221 ccgctttttt tagatccttt tgttgtaaca acttttttgt gtcatttaaa gttgaaatac
    11281 tagactcgaa caccttttgc gccatagtga gctcagtatc tgttttctga atattttcgt
    11341 ggatagaaat agcagataga tcattttctt ggatcaacat ggccatttta aaatctttaa
    11401 atccattata accaagtttt tttgtaaatt gaaaaaaagt agagtcagca atgcccagtt
    11461 cacttgctaa attgctaata ggactatgac ttactttgct tggattttct aaaatataat
    11521 cggcgatagc ctgttctttt gggctgaagt cgttgtacat tgttttgatt tgtagtctta
    11581 tggagacgct catgagaatt cctcattttc ataataagat tatcattttt tccgttctca
    11641 ttaatgttaa agatacaagg agagtaggaa aaaatcaaac aaaaaatatt tttgtaaaat
    11701 ctatttacaa aaaaattttt ttgtataatg agggtataag aaatggaggc gctatcatgg
    11761 aaattggtgt aattggtttg ggaaaaatgg gattgaattt agctttaaac ttaaaagagc
    11821 accatcacaa agtacaagga ctcgacattt caagtacagc tgttagtcag gcaagagaaa
    11881 atgacattcc tgcttatcaa gagatctctg aatttctagc tacttttgag cagaaaaaaa
    11941 tcatctggtt aatgcttcct gctggagaga taaccgaaaa catgttaaga aaactttttc
    12001 caaaactcgg accaggagac attattattg aaggcggaaa ctcttattat aaagattcaa
    12061 tccgtcgagc agaagagttt cagaaaaatg aaatcggtta cttggattgt gggacttctg
    12121 gaggaatcga aggagcacga catcaagcct gcttaatgat tggaggagat aaagaaactt
    12181 ttctatccgt ggaacaatta ttcaaagatg tagcaattga aagaggatat ctgtatgctg
    12241 gtgcatccgg aagcggacac ttcttgaaaa tgatccataa tggaatcgaa tatggaatga
    12301 tgcaagcaat cggtgaagga ttccaacttg tacaaaaaag tgagtatgct tttgacttgg
    12361 aacaagtcgc acgtgtttgg aatcatggtt cggtgattcg cggctggctg atggaaatca
    12421 tggaagatca gttaaacgaa catcccaatt taagtaatta tcggggaatc gtttctgcat
    12481 caggtgaagc aaaatggacg gttgaaacag cgctagaaat ggctgttcca gttccagtaa
    12541 ttgcaactag tttgttcatg aggaatcttt cacaggaaga agatagtttt tcagcaaaag
    12601 tagtttcagc actaagaaat gggtttggcg gacatgaaat agttgaaaag ggtgagtaaa
    12661 atgccagatt atcatatagc aatcgttaat tccagcagtt tcggaagagt ctttccacac
    12721 cacttggaaa gattaaagtc agttggagac gttcatttct ttactgttga tcaagagatt
    12781 ccaggaaaag aactagcgga gttacttaaa ggattcaata tcattattgc aagtgttacg
    12841 ccattttttc gacgagattt ttttgaaaac aaagatgaac tgctattgat ttcccgacat
    12901 ggtattggtt acaacaatat tgatttaaaa gcagcaaaag aacaccagac aatcgtatct
    12961 atcatacctg ctttagttga acgaaatgct gtagcagaaa ataatataac taatttgttg
    13021 tccatgcttc gacgcacgac acaagcacaa gcaagagtca aggacgatcg atgggaagat
    13081 cgggcggagt tcgtcggtag gacgctgttt aacaaaacag ttggtgtgat tggtgcagga
    13141 aataccggaa gcggagtaat cgaaattttg cgtaacggtt ttcgctgtga tgttctagct
    13201 tatgatcctt acaaatcaga tttatacatc gaaagctttg gtgcaaaaaa agtagaactt
    13261 caagaattac tggaacaatc agatatcatt tgtttatgtg cgaatttgac agaagagaac
    13321 tatcacatga tttctgataa agaagtcgat cagatgaaag aaaatgttta tctatctaat
    13381 agtgcaagag gcgctctagt tgatgaagaa gcgatgatta atggtctgaa aagcggaaag
    13441 atcgcaggat atgccacaga tgttttagaa gaagaaccgg gacgtcaaga tcatccgtat
    13501 ttagcgtttg aacaagtagt gattacgcca catatatcgg cctacacaag ggaatgttta
    13561 gaagcaatgg gtgaaaaatg tgtcagagat gtcgaacaga tcagtcagca ccttcttcct
    13621 gaacgttctg tccagacaaa gagcaactat ataaaaggat gatgaagaaa atggtgaacg
    13681 gtttaaaagt gaaaacaggc cctcaatttt atttatatga agaaggtggt atttcaaaag
    13741 tttctgatct cttgaaatca tacggcgcga aaagagtact ggtcacccac ggaacggtat
    13801 catgggaaaa ggcgctacct aaattagtat ttttgaatga cgaaacgatt caattttttt
    13861 atcatcgtta ttctggagaa tgtagttatg ctgaagccag acgaatagct acaatcatca
    13921 aaaaaatgaa atcgatttcg tagttggcgt tggcggagga aaactgaccg atctagtggg
    13981 ttatagtgcc aatcttgcaa gaatcgattt cggagtcatc cctactttag ctagtaattg
    14041 tgctccatgg acacctcttg ctgtcatgta taaggagaat ggacaagcag aagggaaaac
    14101 agaacacttt tacagacaag ctgctttttt tataacagat ccccaattag tgattgattc
    14161 ccctgttcca tatttcattg ccgggttagc agatacacta gcaaaatggt atgaatctga
    14221 gctcatctta gaacaagatt ttttgcaaga tgaatcattt ttgaagctag cacaagatac
    14281 tgcgaagatc tgcaaagaag agattttgaa tcattctgct aaagcaatcc aagatatggc
    14341 acaaagaaaa ctgacatcgg agttcaaaca gctttcagag attatctttg ctgttgctgg
    14401 tttagttgga ggattaggag ataaatacgc aagaaatgcg gcagcacatg ctatgcatga
    14461 cgctatcagt aaatatattc cggaaagcca tcgttttcta catggagaaa aagtagcgta
    14521 tgggattttc tatcaactgg cactagaagg tcgctgggaa aaaatagacg agttatatcc
    14581 tttgtatcgg gaattagaac tgccggtttc tctagaacaa atgggattat ttccgaaaga
    14641 taaacagata atagataaaa tggctgaatt tattgattcg aaagagaaag tgcatttgat
    14701 tcctatagag gtaacacaga aatcactgat acagtcaatg atggaactgg aaggttatat
    14761 cagaaagatc tgaaggagga ggacacatgg aaacattaag tgtagtacaa agtttgttga
    14821 ttgctttatg ggtagccgcg gttatgtccc gctggttagg tggaggagcc acgttgacct
    14881 tacgtttttc tcctttgatg actggattag tcgtcgggtt gatcatgggg gaagttgcac
    14941 aagcgatgat tgttacagcc gctttgcaaa tgatttatat gggggttttt tctccaggag
    15001 gatcaatgcc agctgaacca tctattgcag cagctatcgc agtaccagtc gcattattag
    15061 gggatctgtc gccagaagca gcgattgccg ttgcggtgcc agtcggattg ctcggaagtt
    15121 acttgtatca gtttcgtttt ttcatcaata cttttttagg taaatataca gacaaagctg
    15181 tagaagattt gaatacaaaa gctatggccc gatcaattat tctttatcca acggttgcct
    15241 catttttatt attcgttccg cttgttttca ttgctttata tttaggggcg ccattgatcg
    15301 ctgatgtaat caaagcttta gagggaacag tcgttatcca cattcttgaa gtagtaggtg
    15361 gagggttagc ggcaatcggt atcgcgacaa ctgtatacgt tatcggtcga aaagattatc
    15421 tggtcttttt cttccttgct tattttatga gtgttgtttt gaaatcttta gatattacga
    15481 tggttactta tgccattttt gggattatta tcgctttgat ctttgtccag tcgcaaaaag
    15541 ggaaacaagt acagcctgcc acaagcaata acaattcagc atcttttgat gacgaagatg
    15601 actatgatga cggattttaa tttaggagga atgggaaatg gctgaaataa tggataatca
    15661 aacaggtgct cctgaagaaa tcacgaaaaa agatgtaaca aaagcctatc tgagatggca
    15721 ttttgcaaac gaaattcctc actcttttga acgttacttg gcgccatcac tactatacgg
    15781 aatgatgcca atcttgaaaa aactttataa agatgaagac tctttaaaag cagcatataa
    15841 acggcagtta ctgttcttca acactcaatt aagctggggt ggcggtgtga ttactggcct
    15901 aatgtcttct atggaacagc aaagagcaga agaagagtat gaagaaaaag agatcttgat
    15961 gcaggatgac ttgatgtata acacgaaagc gggattgatg ggggcactag caggaatcgg
    16021 agatgcaatc gattcaggaa ccgtccaata tatttttatc gcaatcgcgg taccttgggc
    16081 acaacaggga agtcctttag gcgcactttt tccttttatc tgctttgggt tgtatcaggt
    16141 tttactaggg gtatttttcg caagacaagc tttcactatg ggccgtaatg ctacaggcct
    16201 aatgcaaaac actggtgttc agacagcgat tgaaatgcta tcagttttag ggttgtttat
    16261 gatgggggtg cttgcgggaa actatgtaaa agtagaatct tcattgaaat ttgcgatatc
    16321 cggacgtgag tttgttatcc aggaaattct tgatcaaatc gtgccaggga tattaccttt
    16381 agctgtcgta atgggtgtgt acttgttcta tacgaaaaaa ggattgaaag tcacacaagc
    16441 attattatgg ctgactggaa tattaattgt gctagcagga atcggtattt tataaaaaga
    16501 agggaacgtg aataagatgg gtgtagtcaa tttagcgcgt gtggatgagc gtttgatcca
    16561 tgggcaagta atggtaacgt tatctcaaaa aagtggagtg aattccattt ttgttgtaga
    16621 cgaagttgta gcaaaagata aatttatgag agatctttac aaaaatgcag gaagccgtac
    16681 aggacaaaaa acaattgtca ttacaccaga gaaagctaaa ttctattggg atgaatatca
    16741 atttaaagaa tacaattgta ttttgatcgc aaaaacagtc caaacgattt acgacttagt
    16801 aaaacacgga atccccatgg aagaattgaa tattggaggg attgctcaaa aaaatcctga
    16861 tacagatctt ttagtgacga aatctgttta tttaaataaa tcagatgctg aaaaattgaa
    16921 agaaatgaat gaacaatatg gcgtaaagaa tatttatttt caagccactc catctgcacc
    16981 taaaacagga ttaaaagatg tattgaacaa gtttgatttg tcataaacaa atgcttagaa
    17041 aaaagataaa gagggcgaaa aaatgaagaa gaacgagctg tttcgtgatt gggaatttcg
    17101 ttatcgctat atttaccgta agcgccgtac aaaaaagagt aaacagcgtt ttctttcagc
    17161 attagtatca gatatttaca gtatgagaac agatgtgaca gtcattgctt acgatacgct
    17221 agcctaccga tcaaaaaata tttatgttgg agatatcgaa aaagcagaga aagttatctg
    17281 tacttattac gatacacccg ttcatgcatt aggaagttat ttcatgtttg attggaaaga
    17341 ccaaaggaaa aaaacaatct actctatatt attgtccttc attttgttgt tcagtttagg
    17401 ttggtgggga atgatgatat ataacaaaaa tcctcatcac gtattcgatc tgctatccgt
    17461 ataaacaggt atcactgtac tagcatttgg gagctacttt ttcttattgg gaaaagcagc
    17521 tcgcggatgg cctagcagac aaacttttat tcgaaatact tcatccatat taacgatgct
    17581 agaaatgatc cgtacgattg atgatccgag tgtagcgtat gcgtttgttg atgaaggctg
    17641 ttatggagaa aagggcttag acagcgtgag atcgggtatg aaaaaagagg ctattctttt
    17701 ttatctagac tctgttgggg cagatacacc gctacagttt tcgggaaatt atttttcaaa
    17761 taaagaacag tggctgaaac aagttgataa gttgaaagaa aaaaacgtca attatatttt
    17821 tagtgcaaga aaaaagcaag cgcagttctt ctatttgacc aaaacagatt tacgagggaa
    17881 aacatttaat tggcaaaatg ccaatcagat tattgctctt tttcgataag taaaaagtgc
    17941 aaaacaagga gtgagaagac taatgttgga aatcatgatc gttacacatg gtaagttgag
    18001 cgaaggactg aaagatgcgg cggaagtaat tgtcgggaca tcgagccata ttgaaacaat
    18061 gtgcttagag tctggggagg acatccaaac attcggagag aaggtcaaag aaaaattaac
    18121 tatgatcaac acagcagaac ccatgctagt atttgttgat ctaattagcg caagtccgta
    18181 caatcaagcg ctcttggcag tcaatgaact gactgaagaa aagaaagcag ccacttatgt
    18241 catagggggc gtgaatctgc caatgttgtt agaagcaatc aatcatcaga tgcttcacac
    18301 accgatagaa gaagcggtgg aacaaattgt caaacaagga aaagataatt taactgtttg
    18361 gcatgcttca gcggaagaaa aaaaattaga tgaggacgat ttttagaagg aaggctgaaa
    18421 agatgaagtg gggatttaga tggtatggtg aaaaagggga ttcgatccca ttaaaatatg
    18481 ttcgccaaat accgggaata aatgggataa ttggaacttt gctgactaaa atgcctggag
    18541 atgtttggga gatttcagaa attgaggagt tgaaagcatc tgttgagagc caaggactgg
    18601 aactgcttgg aattgaaagt gtagcgatcc acgatgcaat caaggcaggg acagatgaac
    18661 gagattacta tattgataac tatatacgaa caatcaaaaa cttgtctcga tgtggggttc
    18721 gattgatttg ttatagcttc aaaccaattt ttggatgggc gaaaactgat ttgtttaatg
    18781 aaaacaacga tggaagctat tctcttttat acgatcagaa aatcgtcgat cagatggagc
    18841 caggagaaat gtatcaattg atacacagcc agtcaaaggg attcaaattg ccgggttggg
    18901 aagaagaacg gttgaaaaaa ttcgctcagc tggaaaaact gtatgaagga atgaccgaag
    18961 aagaattatt tgaaaatctg acttattttt tagaaaaagt gattccagtg tgcgaaaaag
    19021 cagatgtaaa aatggctatc catccagatg atccgccatg ggaacttttt ggttttccta
    19081 gagtgacgaa aaatcttggc gacttgaaga aaattctaaa tgcagtagat tctccatata
    19141 atggactaac gctgtgcaca ggatctttag gggcaaatcc ggaaaacgat ttagttgaaa
    19201 tcatccatga ggtaggttcg cgcatccatt ttgtccactt cagaaacgtg gagtttttag
    19261 gagaaagaaa gttcaaagaa acggcacatc cctctacaga aggctccctt gatatgtatc
    19321 atattatgaa agcgttggtg caagaagggt tcgacggtcc tatacgccct gatcatggta
    19381 gaaccgtgtg gggtgaagtg gctatgcctg gatatggcct gtatgatcgt gcaatgggac
    19441 tcacttacat gcaaggtcta tatgaggcga taagtaagga aaaaccactg ggagagcatt
    19501 atgaataaaa tcaagctctt catctttgat atggatggcc ttttattaga aactggacgt
    19561 ttagcttatc gtgcttatgt aaaatcagca caaaagtatg attatgaaat gcgtaaggaa
    19621 gtctattatt tactaactgg ccaaacagaa atggcgataa gagaacaaat gggcattttg
    19681 tacggagaag atgttccata tatcaaatgg cgagaagcaa tcaatcaata taaagaaaaa
    19741 atcgtgaaag aagataagag agtatatacg aaaaaaggag cagaagaaat tttatctttt
    19801 gcaaaagaaa gaggaatcca tacaatcgtg gcttcgtcta atacacgggt aaaagtagaa
    19861 atgtatttaa aaatggaaaa tctatatgcc ttgtttgatc aaatcatttc aggggatgat
    19921 gtaaaaaaag gaaagccgga accagagatc tttttgaaag cttgttcaaa aatgaatatt
    19981 cctccaagtg aagcacttgt atttgaagac tcgattgcag ggatcgaagc agcaaggcga
    20041 gcgggcattt tgtcttttct catcaaagat catttgatag ggttgcccga tcataatggg
    20101 aagcataagt tgaaagtaga tgtaagttta tatgaatcca ataaagcaga ctatgaattt
    20161 tatgacctgc atcaagcaaa acgattttta gaaagtaaag ggttgtatct ctaataaaga
    20221 ggactatttt ctagtcctct ttatttattg tcctgcacga ctgagggaac agatgaaaaa
    20281 gaagtaattt tttattttga agtatctacc ttatttgtag tgaacaattt agaacaaaca
    20341 caaaacgcat ctactgttat ctaccagtag atgcgtttaa agagaagaac gagaatcgtc
    20401 catatgaggg tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc
    20461 gtaacacctg tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa
    20521 tctgtatcca aggttactcg atattgtata tctttgttat agaatgtcat agccattctc
    20581 ctttctttaa atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa
    20641 gtataccacc aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat
    20701 aaaagcaaat aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc
    20761 gattcgaaga caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt
    20821 agaaaaagca tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat
    20881 ctacatagat aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt
    20941 gatacgtatc aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga
    21001 caaaggtttt ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc
    21061 tatccaaggt gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga
    21121 agatggtcaa cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt
    21181 taaccgtctt ttcgaaagaa aagacggttt ttttgtacga aaaaat
//
LOCUS       DASHFF010000047        20833 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.047, whole genome shotgun sequence.
ACCESSION   DASHFF010000047 DASHFF010000000
VERSION     DASHFF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.047"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(239..523)
                     /locus_tag="U2I75_001907"
     CDS             complement(239..523)
                     /locus_tag="U2I75_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697029.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(541..1431)
                     /locus_tag="U2I75_001908"
     CDS             complement(541..1431)
                     /locus_tag="U2I75_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN1697030.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1451..1669)
                     /locus_tag="U2I75_001909"
     CDS             complement(1451..1669)
                     /locus_tag="U2I75_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN1697031.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1639..3339)
                     /locus_tag="U2I75_001910"
     CDS             complement(1639..3339)
                     /locus_tag="U2I75_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN1697032.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3606..4379
                     /locus_tag="U2I75_001911"
     CDS             3606..4379
                     /locus_tag="U2I75_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697033.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4460..5029)
                     /gene="thiT"
                     /locus_tag="U2I75_001912"
     CDS             complement(4460..5029)
                     /gene="thiT"
                     /locus_tag="U2I75_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN1697034.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5095..5197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5203..6174)
                     /locus_tag="U2I75_001913"
     CDS             complement(5203..6174)
                     /locus_tag="U2I75_001913"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1697035.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6268..7641)
                     /gene="glmU"
                     /locus_tag="U2I75_001914"
     CDS             complement(6268..7641)
                     /gene="glmU"
                     /locus_tag="U2I75_001914"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN1697036.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8144..8962)
                     /gene="purR"
                     /locus_tag="U2I75_001915"
     CDS             complement(8144..8962)
                     /gene="purR"
                     /locus_tag="U2I75_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN1697037.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9129..9953)
                     /locus_tag="U2I75_001916"
     CDS             complement(9129..9953)
                     /locus_tag="U2I75_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1697038.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9926..10606)
                     /locus_tag="U2I75_001917"
     CDS             complement(9926..10606)
                     /locus_tag="U2I75_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1697039.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10629..11567)
                     /locus_tag="U2I75_001918"
     CDS             complement(10629..11567)
                     /locus_tag="U2I75_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN1697040.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11812..12675)
                     /locus_tag="U2I75_001919"
     CDS             complement(11812..12675)
                     /locus_tag="U2I75_001919"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN1697041.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12817..15390)
                     /gene="mprF"
                     /locus_tag="U2I75_001920"
     CDS             complement(12817..15390)
                     /gene="mprF"
                     /locus_tag="U2I75_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1697042.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15531..16337
                     /gene="proC"
                     /locus_tag="U2I75_001921"
     CDS             15531..16337
                     /gene="proC"
                     /locus_tag="U2I75_001921"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN1697043.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16416..17972)
                     /gene="rny"
                     /locus_tag="U2I75_001922"
     CDS             complement(16416..17972)
                     /gene="rny"
                     /locus_tag="U2I75_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN1697044.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18383..19432)
                     /gene="recA"
                     /locus_tag="U2I75_001923"
     CDS             complement(18383..19432)
                     /gene="recA"
                     /locus_tag="U2I75_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN1697045.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19541..20782)
                     /locus_tag="U2I75_001924"
     CDS             complement(19541..20782)
                     /locus_tag="U2I75_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN1697046.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT         5931 a         4514 c         3589 g         6799 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttactta ataccgagaa aatggttttt gaacatcact tattcaaaat
      121 aaaaaaagat tgtgacacga attttgtcac aacctttaaa tgtattccta gagcaaagaa
      181 gctaagcgaa gaaatccaat tctgattatt tattgtattt ctttgttcag catccttgct
      241 aaaagcccat attggacaaa gtattcacta cacccatgat ctcatctttt cgagaacgtg
      301 ctttttctgt cggatcttct acctcttctg tgtcaattcg aacaataaat ttccgattca
      361 tcgtcacgta agcaccgtcg ttgatcaaaa taaacagttc gttctcatta tagtctgcac
      421 aggcatcttt ccatacgtca aagccgtttt tttcagaagg gatatcccgt ttaaccacag
      481 tttttccact taattcgtgg aacgttacgt cataatattt cattatctct acctctttcg
      541 tcattcaata ttttgtactt gttctctgat tttttctaag atcgtcttag actggacgac
      601 ttgattttta atttcgattg catttgattt ggaaccgatc gtgttgattt cccgattcat
      661 ttcttggatc aggaaatcta attcccgacc tacaggacat tgtgcttcga taagttcatc
      721 tagtttttgt acatggatcg ccagacgatc cagttcctcg tgaatatctc cgcgttccaa
      781 caagatggct agctccgtca acaaccgttg ctcatcgact ttcgctccaa gaaattcctc
      841 cagctttttt tgatacctta tttgaaattc cttttcatag atttctacaa aactatcgag
      901 tttttttagt acttctttga attcttcagt attctttttc aacactaaag ctaaagcttg
      961 accttctttt tctcgactcg cctcaataga atcaagtgcg cttttcaatg tatctagagc
     1021 taactgtgcc aattcttcca tgctctcttc cggtttttct tctaccgtta cgaagttttc
     1081 gtccagcgcg aagcgttccg ccatccgact aattgacact tgattttgac taccgtatcg
     1141 ttgttgaatt ccgtctgtca attgagtgat aagtgaatca atcaattccc agttgatgac
     1201 gatctgtttt tcgtttttgc caatataagc tagattcaca aatacttcga ttcgaccgcg
     1261 gcttaactgc tgtttaataa gctgacgaaa atcgttttca acaaagttca ggattttagg
     1321 acttcgtatt tgaacatcta gaaaacgctg gttgacactt tttatttcaa tatctaactg
     1381 atacgctttt gtttcgcgga tggctttgcc aaaaccagtc atacttttca ttcgacaggg
     1441 tccttcctca ttacatattt tctattgctt tttgcagttg ttcaaactct tcgttcgtca
     1501 atgtcaggcc tttccccatt ttttcatggt ctggcgccca atctcgaaga tcgaattttg
     1561 gcgggcgacc attccagctg actaggttca gttctttacg ccaccccttg ttgttttctg
     1621 acaatatagc gatttcttct acgatttcat atgaaaattc ttgtgccatg gttgttcctc
     1681 cttgagataa tataaatata ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg
     1741 ctgcaaacga tccttgcgtt ttccttgtag cagataatca aaataaacag ttttcgtgcg
     1801 ctcattttgt attttttgct ggtattttcg ataccttcgc cattcttcag tacttaattg
     1861 accaatttgt gccaataaat catccggcat actttttata tcttgttggc agatcatttg
     1921 aatcatcatg tctacttgat agatttcatt gggcagtaaa aaatgttttt ttgctaaaag
     1981 ttctagcatc gccaatccgc atgtctctgg tacgaccctg ttcttatata aattacgcag
     2041 tttttgccaa aattttatgc ttttgaaaaa agataattct aactgaattt cttttgttaa
     2101 gtaagacaaa aatcctcgcc agtatgttct tgcaggttta acagaatgaa atgtatacca
     2161 gttcccatag taatagccta atcgtttttt catttccaga tagcctaatt gtaaatttaa
     2221 ttggttcctt tggctatcaa aaagcagaaa gctgcctagt gtccacaatg tttgacattt
     2281 ccaatggata aaaatgtctg gcatacgaat ttttttcgct gggcctggcc cttgcacatc
     2341 tacgacaaat gcctctgttg ctcctttgtt aatcgcaata tcaataggta ttttgttgcg
     2401 atacccaccg tctgcatatt tattttttcc gatctttcga taagccatag cgggataaaa
     2461 tgaagcagag gcgagtatcc aatctggtat ttcttctgtt tttgtctgtt ggatatcagt
     2521 cactacttcc gctagttctg gcagtttagt agacaccgta tataaacgta ttttgggatt
     2581 atgtttgagt aaggacaaat ccaaattgtc tttgaccatc ttttccaatg gagcagaaga
     2641 aaccccgcct tcaattagtg ctgttttcgt catttggcgt ctttctcgat agaagcgttc
     2701 acgaaggtct tcaactgcag ccatttctgg taacgcaagc acttgtcttg tcgacagctt
     2761 tttccataaa ttcacagctt ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc
     2821 acctaccgag gtacctgtga taatctcaaa gttgatatca tgttccttca gcgcctgcca
     2881 cacaccaatc tgataagcac cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata
     2941 gaccagcttt ttatttaatt gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa
     3001 ccattcttct gatagaggag attgaaataa aaaacaacag ttttctttaa taaaaaaacg
     3061 agcgcaagct tccatatacc gaaaaaattt tttccattca aaagtttcac cttcaatcaa
     3121 cagattatta aaaataacat ctcctctact tttagcaata gtaaaataga cggtatattt
     3181 tttcgatact gctttaaaag cacattgctt ttttgcagaa cgccaaaaag cgtctctact
     3241 ttcttgatga ctttgaaaaa atggaagggg ttgttttgct tgctggatag gcgaatacag
     3301 cgacattttc tgtgaaaagt aaactttttt tatctccatc gcgctctcct ttttcttcaa
     3361 gtataccagt acgtcataaa aaggtcatga gtctcgtatt cgactttgta gattccataa
     3421 gaaaacaatt cataaaacct cctttaaata aaaaaataca aaaatcacaa aaattaattg
     3481 aaaatgagag tgttttcaat gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa
     3541 caagcttttt taatgcaaat gttgtaaaat aaaagagatt aagaggaaat aagaggagga
     3601 acatgatgac tgagtcagtt tatgtacata ttgataccac aagtaatgct gttttaacaa
     3661 aagggctgac tgctactgac tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct
     3721 tgcttgatcc atctgcttct gctggagaat atgaatccca tacagggctg aaagtaatcc
     3781 gtggtacaga aaatattcag cgtttctttt cctcttctcg taacaggcgt ggattcgatg
     3841 aattgaagtg gatcgacttt actgatttaa cgatgctgaa agaattgaca cctttagaaa
     3901 tttcagaatt attatatttt ggtcatatga aaacacatct tcattcgccg tttttttata
     3961 aactacagaa taattttgta tacttcgatc ttaatgatca gctgaatcgg atttattatc
     4021 gctatttaga tgaattttat aggatttttg ctagtaaatt aagttcgatc ttgtcagata
     4081 agatcaatgt aaaaaaatct tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg
     4141 aattattgaa agaaatgcgt aatttgatgc aggaagggat cattttttct tttgatcaag
     4201 ttggtttgat cgatggtaaa tatcgtatcc caatctatgt gattgaagat tatatttgga
     4261 aattaaaagg cacccggtat cgagaagagg aaatcatcgc tactttaatc tactctgtca
     4321 ccgatcgaca ctggcaattg gtagaagatg ccgatctttc ttatctgcca ttgaagtaga
     4381 tctatttctt atcaaaaaat aaaaaggaaa caagaaacta ggacatgatt ctgtctcaag
     4441 tttcttgctt cttttttatt tacagctcct gtcgtttatc tggcacaaaa atcgaaggag
     4501 tcgtttgttg gatccctaat aaaacgatga tcgttacaat agctgtactc aacccgctgg
     4561 caccattcat tacgaaagaa aagagccaag gattcatccc ccatagtgca tacgctcccc
     4621 agaaaaccac tcctgcaata aaatgccaaa aatatctgga aagtgcgcca atcaatgcac
     4681 caaacacgat gctttttcta atttgtttcg tattatcgtc tacatagcct ttttggacat
     4741 ttgaagccat gagccctgct gcgcctgcaa aagtataggc aatcgggtac tcaatcaaca
     4801 cttgaacgac acttaaataa taaacttgtc ccaacggaaa atgtaggatg ccccaaatga
     4861 aacctgccag catccctgcc tttgtccctc ttcttaacgc aaaaagagtc aatggaatct
     4921 gccctaatga aatagaaaaa ctgctgccga tctggatcgg aatcaacgac aacaccatcg
     4981 ctaacgctgc aacaatcgtt ccttctaccc aaactctatt ttttcccata aaaaaacttc
     5041 ctcctttgaa taaaaaagtc acaaaggagg aagcagcttt cgaaaatagt caaacagctg
     5101 tttattcaca attcctacgc tcgcataacc gatacaggta cgaagggttt agaaatagat
     5161 tcaatctcag cccaattgga ctcccctttg tgagtttatt tattatttat tttttgtttc
     5221 aaacaatgga ctaactggtt tgttttcatg gatacgtttg attgcatctc ccattagtcc
     5281 gccaacactt acttcatcga ttttgtcgat tttacgatct tctggaagat agatcgagtc
     5341 tgtaacaact agacgctcga tagctgaatc ttctattctt tgtaaagcag ggccggataa
     5401 tactggatgt gtacatgaag catacacact agttgcacca gcttctttca aagcattggc
     5461 agccaatgtg atcgtacctg ctgtatcgat catatcatcg atcagtacac aagttttgcc
     5521 tttgacttgt ccaatgatat tcattacttc tgcaacattt gcttttggac ggcgtttgtc
     5581 tatgatcgca atcggagatt tcaaaaattc cgctaattta cgtgcccgtg tgacaccacc
     5641 atggtctggt gaaacaacga caacatcatc gcctttgatt cctttttcta agaaataatt
     5701 ggcaataagt ggcgcaccca tcaaatgatc gactggaata tcaaagaaac cttggatttg
     5761 aactgcatga aggtccaaag tcagcattct tgttgcacca gctttttgaa tcatgtttgc
     5821 tactaatttc gctgtgattg gttcccgtgc acgtgcttta cggtcttgac gcgcatagcc
     5881 ataataaggc atgactacgt tgattgtttt tgcacttgct cgtttcagtg cgtcaatcat
     5941 gatcagcaac tccatcaagt tatcattgac tgggctgctc gttgattgaa taacatagac
     6001 atgcgatcca cgaatacttt cttcaatgtt tacttgaatt tctccatcac taaattgagt
     6061 aacagatgat tttcctaact cgacgccgac tgctgctgca attttctctg ctaatgggcg
     6121 attggaattt aacgcaaaaa tttttaatct tgggtcaaag taatgttttg acatggggac
     6181 ctccactttc tttttcacaa ctctagaatt ctacctaata aatagtagtc atatttttta
     6241 aataaatcaa gtgattttgc aaaagtctta gtttagataa gggagttttt ttgcatatcc
     6301 ttctttattg acttgtcttg ctctcgcaat tgccagcgca tattctggaa tattgtctgt
     6361 aattgttgac ccggcagcaa tcgaactgtt tttagctacc tcgacaggtc cgatgatatt
     6421 cgtgctagaa ccaataaagc tgtggtcgcc gacagtcgta tgatgtttgt tctttccatc
     6481 atagttgacg aagacaacac cacatccgac attgatttct tctcccagcg tagcatcacc
     6541 aacataagtc aagtgaccga ctttagttcc tttgccgatt tgagcatttt tcacttccac
     6601 aaagtttccg atatggacgt tttcgccaat ttcagctttt ggtcgtaaat gtgcataagg
     6661 tcccacatct gcatgtttct tgacatggct gctttcaatg actgagtttt caatgacgac
     6721 gtgatcctca atcctactgt ctacgatttt tgaatgtgac ccgatgacac aatcagaacc
     6781 aatgactgta tttccttgga tttgtacacc tgcttcgatc aatgtgtctg gaccgatttc
     6841 tacgcctgca tcgatgtatg tcgttgctga atcgacaaag cttacgccat tgaccatatg
     6901 catctggtta attcgctttc tcatgatctc atttgcttta gctaacgcga tccgatcatt
     6961 gactcccatt gattcttcaa aatcatccgt ttgataagca gcaactgtat ggccttcttc
     7021 ttttaagatt tcgatgatat ctgttagata gtattcacct tgagcattgt ttgttccaac
     7081 tttgcttaac gcatcgaaca aagcttcgtt gtcaaaacag taagttcctg tattgatttc
     7141 ttgtacaaga gcttcttctg gtgttgcatc tttttgttcc acgatttttt ctacgatccc
     7201 aatatggtca cggatgattc gtccatatcc tgttgggttt tctgcttgtg ccgtcaagat
     7261 cgttgcactt gcattttttc cttggtgata ttcaaaaaga ttgttcagcg tttcagtagt
     7321 caataatggt gtatcgccac tgataactaa cgttgttcct ttttttcctt ccaagaaaga
     7381 agcagcttgc aaaacagcat gtcctgttcc caattgttct gcttgtaacg catatttact
     7441 gcgttctcct agttgtgctt taacttgttc tgctccgtgt ccaacgatcg taatcacttc
     7501 atctggtttc gtttcactga cccgattaat gatatgttct accataggtt taccagaaac
     7561 gggatgtaaa actttatata attttgactt catgcgggta cctttccctg ccgccaaaat
     7621 aattgcataa cgtgcgtcca actgcgtcac tcctaaatat agtttgctga tttcagttta
     7681 tcccagtata cccatttttt caacaaaaca agtgattttc ttttatccca gcgttttctg
     7741 gataaaaaaa tagatagacc ataacgggcc tatctatttg gtaaccggtg ctctcctgcc
     7801 aactcctcgg tcacaagtca cactactcat aaaacatgaa aagctgtttt ttgaaatcgt
     7861 ttcttgtgac ttagagttaa acggtaggct tacaacctct tcattaaacg tttttcaatc
     7921 agctgacctt cggtattagc tcaataaacg gcaaaatatg aggagcaatt tttccgttta
     7981 ttgagctaat actcaggaca ggacgctgat ttcacaacct ctagataaac ggtgttcaaa
     8041 agccagtttc tcggtattaa gccaaattta tgaaaaaatg tggaaagcca ttttcacaaa
     8101 atttttctta ctactcgaaa ctaagcagct ttttcacaac ctcttattct gaaaaataat
     8161 ttcctggcac aaccgtaata tttttcgttt gagtatctac atctttgaca taaagcagtg
     8221 acgtataatc atcgatggcg cgatgaccgt tgaattttga ttcggcaaat acagtgatgc
     8281 caacaagttc tgattcgaat tcttcaatca agctcttcat tccgttgact gttccgccgc
     8341 ctttcatgaa atcatccact acaaggacac gtgaaccacg tttcaaactt ctcttagaca
     8401 gttccatctt ttcaattctt tcagaagatc cagacacata gttgacactt actgttgatc
     8461 cttctgttat ttttgagtcc cggcgtacaa taacgaatgg cgcattcaga taataggaca
     8521 cagcttgggc aataggtacg ccttttgtgg ctacagtcat tacagcatcg attttttcac
     8581 caaggtattt agaagcaatg attcttccaa cttgacgcag cagttcaggt tctcctaaca
     8641 aatcagagag ataaacatat cctcctggaa gcagacggtc ttgttctgat agacgttctg
     8701 ctagagattc aatatatttt ttcgcctctt catatgaaat ttcagggatg aacaatacac
     8761 cacctgcagc acccgggatt gtttccaagg ttccataccc tcgttcttta aatgttttct
     8821 tgatgattcc caaatcttca ctgatagaag atttagcaga ttcataacgt tcggcaaagc
     8881 ttgttaagga aatcaattca tgcgggtgat tcaataaata ctgtgtcatg tcaactagac
     8941 gttcactgcg acgtactttc acctattcca actcctttta agaaatttta tcgttttaca
     9001 ttatataagt ttttcttcaa aaattcgagc attttatgaa aaaataagaa aatagttcgg
     9061 tatttattaa aataaaaaga agcctataaa aaatttttcg tttttataag gcttcttttc
     9121 tttataattt agttattttt tcttctgaag gcaaccaata ttcgtttgat gatatttaca
     9181 atcaggaaca aagcaataaa gactaatgtg attgttgctc ctggaggtgt atctagataa
     9241 aacgagctag ttagtcctga cagcatccct agaaatccta cacaaacact gatagcaatc
     9301 acgacattga agcttttgcc taatcgcata ccgattgctg cgggaagaac catgattgcg
     9361 gatatcaaaa gtgcaccggc aatcgggatc atcacagcaa tagccacacc agtaatcaca
     9421 ttgaacatca tcgacatcaa atgaacgggt agcccatcca cgtgggcggt atcttcatca
     9481 aaagtcagaa catacatcgg ccgtttgaat aaaaagaaca atataccgat ggcaacgaat
     9541 aagattccta aaaagatgac ttgctcccat gtaatggtca cgatcgaacc aaacagatag
     9601 gattggatac ttgttgctga attccctccg ctcaaattca tcaatactaa cgctagcgcc
     9661 aaaccaccag ccattaaaat ggcaatggag atttctgaat aggttcggta gatcgttcgc
     9721 agatactcca atatcacagc tgctaataca acgactagca tggtcgtaag tgtaggatta
     9781 aggttcaaaa agaatcctaa cgctacccct gctaaagaga catgagaaag cgtatcagcc
     9841 atcagcgatt gtcttctgat cactaaaaac acacctagca tcggggcgat tccggcaatg
     9901 aagatagcgg caagaaacgc tcttctcatg aattcatatg aaagcaacgc cattgagaat
     9961 cctcctttct caccagtcgg atctgtctgt ccgcatactg tttgatatct tcatgatcat
    10021 gggtgatcat tagaactgat ttcccatgga catgcgcact atgtcggaga agacgataaa
    10081 attcatttct ggattcttca tccatacctg ttgttggttc atcaagaaca aataagtcag
    10141 ggtcagtagc aaaaatacgc gctaaactga ttcgctgctt ttgtccgcca gacaattcac
    10201 ctattcgccg atttcgcata tcccacatcc ctacagcttc taaagcacgc tgtacatgtt
    10261 gatgatcttt ttcagagaga cgcttgaacc agcggcctct tggatatctt cctgaacgga
    10321 ctaattcgat caccgtacta ggaaaacctg cattgaaaga tgcaacttgt tgaggaatat
    10381 aaccgatact taattttttt ccttcttgat ttttctcagc aatctttatc attccttttg
    10441 aaggtttcag taatcctaac gatgctttca ccaaggttga cttggctgct ccattttctc
    10501 cagttaaaat gacaaattca cccgcatcta catgataaga gacatcttct agtacaggtt
    10561 catcatcata gtaaaaggtc aaatcagata tttcgatata acgcacctta caacctccat
    10621 tctaaaactt aattgatact ttcttttaaa gctttcagat ttttttccat gacagaaata
    10681 taattttcgc ctgctttgat ttgttcatta gtcaagcttt ctaaaggatt caacacttct
    10741 agcttcactc ctgtttcatt cgacaatgtc tcagctacct tcgaagaggc attttcttca
    10801 aaataaatca ctttgatgtt gtttttcttc acaaattctt ttagctcagc taagcgactt
    10861 ggtgtgggtt cctgatctgg tgaaacgccc gaaatcgcta cttgttttaa gtcgtattga
    10921 ttcgctaaat aagcaaaagc agcatgctga gtgacaaatg tgcggtttgt tgcatctttt
    10981 agctcatcgg agtatttttg atccagtgct tcaagctttt tgatataagc atcggcattc
    11041 ttcgtaaaag tttccttttt atctggatat ttttcgccta attcttcagc aatcgttctg
    11101 acttctttta tagctaatac cggatcgagc caaacatgtg gatcgtattc atgtgaatga
    11161 ccatgccctt cttctgattc ttcatgttcg tctgcttctt gcgattctaa acggtcgatt
    11221 cctttagctg cttcgatcat caaggtctgt ttgctatcca ctgaatcctt taacgaatcc
    11281 acaaaaaatt ccatgtctga actattatac acgaaaacat ctgcatcgga aatttctgct
    11341 ctttcttttg cacttggttc gaaatcatgc ggctccgttc cagcagggat caaaagtttc
    11401 acgtttcctt catcccctac gatctctttt gtaaaatcat acatcggata aaaagtcgta
    11461 acgatcgtca aatcctcttt tttgtctgct tccttgttcg tatttccaca tgctgcaaac
    11521 agtaaagcac ctatcattac cgtagctgtc aaaagatatt ttttcattta tatgctcctt
    11581 tcaatgaaca aatcggaatt ttacgattta ttaacgtgaa ctaatgtatc aaaataaaaa
    11641 aacacagtca atagaaatcc atcaatttct gatttaagca tgtatattct tgattaaatc
    11701 gtaaaatgga tgagttgctg ctcatatcta gccatttcac tacaataaaa aactgaagaa
    11761 taccatttta ggattcctca gtttttcatc acctatttta cttcttatat tttattgcag
    11821 ggttcgtact aaataaactt cctcacaaaa accttttaac ccattatata ctctctgcgc
    11881 tcttgaataa tgttgacaca aggcaaaaac ggttggtccg cttccactca tcaatgcagc
    11941 atctgctccg tacttgatca tacgctcttt gatttgatgg atgactggat gccgctttcc
    12001 cgttatatct tctaaactgt ttcccatttt tttgatcatc tgctgataat cttgctctcg
    12061 gatggcttca gcaagtccgt ggatgtcttg atgttggatg gcttgcgtat ctacttctct
    12121 aaacactgta cgtgtagaga cactcattct tggtttgacc aacacaaccc agcactgtgg
    12181 catagaaggt aatacttgga tttcttcccc tttgccaccg acaaatgccg tacttccgta
    12241 gacacagtaa ggaacatctg taccgacttc caaaccgatt tctgccagtt cttctaacga
    12301 acagcccaga ttccataatt ggttgatccc tcttagagca gctgcacagt ctgtacttcc
    12361 tccgcctaat ccagctgcta ctggaatatt cttcttaaca aagatcttga caccggtttt
    12421 gatattgtat ttatttttta gcaataaagc agcttgataa acattgttcc gttcatctac
    12481 cggtaaaaat gctttatttg tttctacaat gattttgtct tctgttattt cttcaaaaaa
    12541 tagatgatct gccaagtcta cgctcgacat caccatttcc aactcatgat acccatcttg
    12601 ccgtctatat aatacatcca gtcctaaatt tatcttggct ggcgcttttt caatcaattc
    12661 catcctctca cccattcgaa aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa
    12721 agtatagctt atgtctgcct agtggttgat tttgtcatcg tttcaacagc tttcacagaa
    12781 ttttctcttc ataatccaaa caagttctag tgattcttat aattctgtga gcacttcttc
    12841 tgccaaactc ggaacatttc ctttttcttt tcctatcaga ctgagtagct gcatggtagc
    12901 aaatccgaaa ttacttcctt tcctataagc aaaataacgt ccttcccaat gatcgacata
    12961 ttttaatttc ttttcataag cagactggaa ggaatagaaa aaattaccat aattatatac
    13021 gatatgcatg actttttctt gaaagaaaga aaaatctgtt tctccaacat tagctaatgg
    13081 agctaagcct agattggctt ccgagtagcc cattttctcg tatacaataa acagattggc
    13141 taacagatag tcagctaatt cctcagattc tttaggtaag acccgcaaca aatcatatcc
    13201 cgcctgcttc ttcgtcatcg gctgagcggt gatgaagcca acgacttttt gatctttacg
    13261 gacaataccg acaccgctag ataatacata tgatttatca aatcgtccca cagagaagta
    13321 tttttctcgt gcaccttgaa gccatttttg tgaaacttca tttacagctt gatgtaattc
    13381 ttccggcagt tgatcgtacc aagtaaaatg gtatccttct ttcatcaaac gttgatattc
    13441 aagttgctga ctcagccgag acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc
    13501 tactttcgtg aagtgaaaac ccaattcatg aagcatcact acgtagcttt ctgatatctt
    13561 ataaaacgca agctgatagc ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc
    13621 ccgcatcatt ttaggattgc caactggatc tcctaaaaca aagcatttgt ttcctttgat
    13681 ctgataacca aaaacgactt gatcttggtc gtcttcttgg taataaaaat aactgtaacc
    13741 tgacaagcga agctgatggc ttgtagctgt gcctccgtat gtttccaata aacggtcaaa
    13801 acggtctttt tcgtactcac tgccaagctt cgtagtatct tcagctaaat attgataaag
    13861 cgccactaaa gctagcaatg agatgcccaa accaataagc cctgagaacc acacatcatc
    13921 tgaaggaaac aaaataaaat gatttcctgt tattcgaccg actaaaaagc gtccgctgtg
    13981 atagcctgca attgcgtaaa cgataaacaa aaagccgaac aagctagcat caaagattat
    14041 agctccccaa gaataaacga attttgttcg ataaaattct tttctggata accacacagc
    14101 taacagaatc aagacatata ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt
    14161 taaaatacca aaagatagta aagcaatcgt aggcagaaat gcctttttga cttttactga
    14221 aagtgctcta gcaaggccga gtaataaaaa gccaaccaat agattcactg tctgatctaa
    14281 aaaattgaat gagaacggca ataatacttg aaatagccga ctgattgcag acagattagt
    14341 gattgttgat agaagtacca tcatgatccc cgcaaaatag acagcactta cgagcacgat
    14401 atgcgcaacc ttttggaaaa acagtcgcgg aatgttatct aaaaatcgat tgattttgac
    14461 tcctgtatgt gtgatgaaca atagaagtcc cgtcataaaa ggtagaacat aataaaacaa
    14521 ccgataatat aatagccaaa ctactgctac atcttgccga acaggcaatt gactcattcc
    14581 taagatcatc aaaacgtcaa aagtccccat tccacctggg accatcgtca gcattcctat
    14641 caatgtagca atcatgaaca taggatagat cgaaagcatc gaaatatcta ttctcatcaa
    14701 cttgccgatg atcaaaaata ccgcaagggc tcccgtccat tgtccaaagg atgccagtag
    14761 cagaccaagc aagccttttg gataaaactc gaagaacaat gtctttctct ttagataagc
    14821 aaaaaagaaa atgactggtg ctaaaaaact accgccgaaa agccagatcc aatagcttcg
    14881 aaaaatactt tctgactgaa taaaaaagac atcaataaat gtaagaaacg accatacaga
    14941 caaaccagtc agcataaata gtgcgacttt tgatacagtg gctaaaacca tttttctatt
    15001 tgtctgcttt ccataaaaac tagcccgcag acttgctcct accacaccac caaaaccggc
    15061 gagattgttg atcgtgtttg ttacccacgc agcaagaaga aactcttttc tttccatttt
    15121 tggttttcct tgtttttcta agactctgat cgtcacccaa tcatacaaca acatcggagt
    15181 gacacccagc agtccagcca gaatcatgag caggatcgtc atgcggcttt gttgtcccat
    15241 cctttgccaa atatcttgcc aactcatgcc attagcaata tttgcgactt gatttgccac
    15301 aaagactaac acggagccga aaaaaatcag ttttaaaatc agactatgtt cctttatcca
    15361 ttgcagcatc aagtatcgtt tatttttcac attttcccat tcctaacttg tatacttttg
    15421 tgtcataccc caatgttatc ttatttattt taatttttcc actttagacc tgttctttgc
    15481 ttgcgatgat agaaaaagaa agatatccta atgctaaagg agaggataac atgaaagtag
    15541 gatttatcgg cacagggaat atggcacaag ctattattga tggtttatta aaaaaaggaa
    15601 cagtctcgaa agaagaggtg ttcgtttcca gtggacattt tgaaaatgca caaaaatttg
    15661 ctgctgaaac tggcgtccat gcatgcaaaa gtaatcagga agttgcatca caatcagata
    15721 tcgttctttt agccgtgaaa ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat
    15781 atagccagaa agtcttattc atttctattg cttcaggaaa atcattaaca gatttagaaa
    15841 cagcgcttga agatcctcag gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg
    15901 taggagcagg cgtttctgcg atttgcaaaa atgaagctgc cacggaggaa cagcttcagc
    15961 aagctacgat gatttttgaa gcaatcggca gcgtctatcc tttagcagaa tcagatttca
    16021 gtacattcat tggccttgct ggcagctcac ccgcattcgt ttacatgctg atcgatgcga
    16081 tgggacggac tggtgtactg aatggtttgt ccaaacagac agccactgag attgctgcaa
    16141 aagcaattat gggttcttgt caaaaattcc tttcaagtga taaaaatcca tgggaacttg
    16201 tagaccaagt ttcttctcct ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag
    16261 caggattcat cccagcaatc atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga
    16321 tgttgaataa aaaataatga tagcaatgtt ttttcgtgcc ttacaaaaaa agaagagctg
    16381 tggcttttgc cgcggctctt cttttgtatt ttttattatt tagcataatc agttgcacga
    16441 gtttcccgaa taacgattac tttgatgtgt ccaggataat ccagttcttc ttcgattcgt
    16501 ttacgaatat cacgtactaa acgaacagcg tctaaatccg agatttcttc tggcttgacc
    16561 attacccgaa cttcgcgtcc agcttgaact gcgtaacttg attctacacc ggcgaagctg
    16621 ttcgaaatat tttccagact tcttaaacga cggatgtagt tttctaatga ttcacttcgc
    16681 gcaccaggtc ttgctgctga gagcgcatct gctgcagcta caaggacaga gataacagaa
    16741 gtagcttcca catctccatg atgagaagcg atcgcgttga ttactactgg gttttctttg
    16801 tacttggcag ctaattccgc accgatctca acgtgagatc cttcaatctc atggtcaagt
    16861 gctttcccaa tatcatggag caacccggca cgttttgcta attggatatc ttcgccaagc
    16921 tctgctgcta agataccagc taattttgca acctcgaccg aatgcttcaa gacgttttgt
    16981 ccataactag ttctgaatcg cagacgtccg atgatcttga ttagatctgg atgaagtgta
    17041 tgagcaccga cttcaaacgc tgcctcttcc ccgtattcgc gaatacgttc atccatttct
    17101 ttacgagatt tctcgaccat ttcttcgata cgcgctggat ggattcgtcc atcttgtatc
    17161 agtttttcca aggtcatccg tgcgatttct cttcggattg gatcgaatcc ggaaagaaca
    17221 actgcttccg gtgtatcatc gatgattaaa tcgataccag tcagcgtctc aagcgttcga
    17281 atattacgac cttctcgtcc aatgatacga cctttcattt catcattcgg aagggtaacg
    17341 actgaaacag tcgtttctga aacttgatct gctgcgcagc gttgaatcgc taatgataat
    17401 aagtttttcg ctttgcgatc tgcttcttct ttggcacgct gttcagattc ttttaccatt
    17461 agagttaact catgattcag ctcttcttca gtcgatttca tgatgacatc tcgtgcgtcc
    17521 tcttttgata aaccagcaat tttttctagt tctttttgtt gatcttcaat cattttttca
    17581 acgtcttttt ctcgctcttc aattaattgc tgttttgaac taagtgcttc ttctttactt
    17641 tcaagtgagt tctcgcgttt ttctaacgag tcatctttac gattcagcgt ttgttcgcgt
    17701 tgaatcaaac gattctcttg agattttaat tctaacttac tttctctcag ctcactttca
    17761 acttcagaac ggtatttctg attttcttcc ttagcttcta acaatgcttc tttttttaga
    17821 gtttctgctt ctttttttgc actatcgatg attccagcag ctgatgattg tgcaccagct
    17881 atttctttct cgtgacgtga ctttgcgacc ataaacccta aaccaagacc gacaattaaa
    17941 ccgatgatag cgaggagaat gttgaatacc ataaaatcca cctccatact atctttttca
    18001 tttttatcat ctatacagct tctatgataa attattctaa actcgtcaat atgcatcttc
    18061 gcatatatgt gtacaaaaaa catgcacaac tttattctaa tgtttgtacc ccgtagtgtc
    18121 aactttaaat aaacaaagaa acggctttct acttattcat taaacgatgg tttttaaaat
    18181 aaatgacact agtttcatat aggtaaacct ttaaattgag aaacttttct attaaattat
    18241 tctttgtaga tgaacagata tttttatttt atgtacccac tcgtataatt aaataactca
    18301 cgatttcttt attcaataaa aaaacggcag gtcaaggaga gtacctactc ttcagaccag
    18361 ccgtttttgt ttatcataat ttttattctt ctactaaagg caattcttct tgtccttttt
    18421 catcttctgg gacagcgact tcttcaccaa taccataagc tgctctaact agagcagaaa
    18481 cttctgccat catttcagga tgattagcca tatagatttt agcgttttcc cgtccttgac
    18541 caatccgatc ttctttataa gaataccaag caccgctttt atcaacaatg tctttttcta
    18601 ctgccatgtc tagaagttct ccttcttgtg agatccctaa accatacatg acatctactt
    18661 cagcgatttt gaaaggaggt gctactttgt tttttacgac tttgatcttt gtacggttac
    18721 caacaatatc tgtaccttgt ttcaattgtt ctgcacgacg tacttccaaa cggatcgttg
    18781 cgtagaattt caatgcgcgt cctccaggag tgatttctgg attcccaaac atcacgccta
    18841 ctttttcacg gatttggttg atgaaaatag cgattgtttt tgttttgttg atcgaaccag
    18901 agagtttacg caatgcttga gacatcaaac gtgcttgtaa cccgacatgt gagtcaccca
    18961 tttcgccgtc gatttctgct cgtggaacta aagcagcaac tgaatcgact actacgatat
    19021 ctacagcccc acttgatact aaagcatcag cgatctctag accttgttct cctgtgtcag
    19081 gctgtgaaag aagtagttca tcgatattca cacctaattt ttgtgcatat tgcggatcta
    19141 acgcatgctc agcatcaatg aaagcggccg ttccgccatt tttttgtact tctgcaatag
    19201 cgtgtagtgc aactgttgtt ttacctgaac tttcaggtcc atatacttcg atgatacgtc
    19261 cacgggggta accgccgaca cctaaagcga catctaacgc aagagagcca cttggaattg
    19321 tagagatttg ttggtcgatt ttttcgccca atttcataat agagccttta ccatagctct
    19381 tttcaatttt tttcagtgca gcatctaagg ctgctttacg atcatctgcc aatacgataa
    19441 tcctccttat atttacaaat cagagcaaca tttcattatc cttatcatat cttttttagt
    19501 gaaaaaaagc aagaaaaaac cgaacaaata ttcgcattta tcattttctt aaaagctgtt
    19561 ttctcaaaag atcaaatcct tgcataaccg cattatatct gatagcagca cgatctctgg
    19621 caaagtgata tttttccgaa accacgccgt ctttagatga aatagcgatc catactgttc
    19681 ctgcagattg accttctaac gtgtccggtc cagccactcc ggtgaaagct acactgtaat
    19741 ctgttcttgc tatctcacgt gcttgaatgg ccatctgctc cacacattct ttgctgactg
    19801 ttccatattc tgctaatagc tccggatcaa tctccagaaa accagctttt gtttggagcg
    19861 agtaggtaac gaatccgcct ggaaatactt cagatacacc agcaatgtcg cctaatgcgg
    19921 cttggaaggc gccggctgtc aaactttccg ctgctgttac agtctgcttg ttttctttca
    19981 gtagttccac tacgactttt gctagcgaat tatcatcgcc gtatccataa aaatattccc
    20041 ctactcgttc ttggattttt tcttctaatg ctaaaagagc ttgattccct gcctgaacat
    20101 catttgtttt cactgtcagt cgtaatgtca cttcatttgg tttggcatat ggtgcgatcg
    20161 ttggattgat ctgtgtctca atcagatctt ttagctcagt gaccagtcgc gattcaccaa
    20221 tcccataaaa gcgcaaaact ttagaaatta gtttttcttc agaaggaaaa cgttcctcta
    20281 gtaaaggacg tacttgttcc acaaacattg gtttcaactc attaggcgga ccgggaagaa
    20341 ggatatacgc atgtgtatct gtttgataaa aagtccctaa agcaagccct gtacgatttg
    20401 gtagaggaac cccgccttcg ataatctggg actgttgcag attgtttttg gtcatttttc
    20461 gatgggttgt ttcaaaatat gccaataatt ttttgtatcc ttccgtattt tgtatgagag
    20521 acttaccgag gtgagccgca gttgcttctt ttgtcaggtc atcttctgtc ggtcctaatc
    20581 cgccacataa gagtatgagt tcacttcttg tttcagctag agcaatcaac tcttctaaac
    20641 gctgctgatt atctcctaca actgtttgat aataaacttc gatacctaga tcagctagct
    20701 gttctgacaa gaaagtggca ttcgtattca cgacttgtcc tagtaaaagt tctgttccga
    20761 cagctattat ttctgctttc attacttccc gctcccttat atagtatacg ctgtattttt
    20821 atattttttt att
//
LOCUS       DASHFF010000048        20224 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.048, whole genome shotgun sequence.
ACCESSION   DASHFF010000048 DASHFF010000000
VERSION     DASHFF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.048"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            194..493
                     /locus_tag="U2I75_001925"
     CDS             194..493
                     /locus_tag="U2I75_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697047.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(590..1024)
                     /locus_tag="U2I75_001926"
     CDS             complement(590..1024)
                     /locus_tag="U2I75_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1697048.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1256..1966)
                     /locus_tag="U2I75_001927"
     CDS             complement(1256..1966)
                     /locus_tag="U2I75_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1697049.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1956..2816)
                     /locus_tag="U2I75_001928"
     CDS             complement(1956..2816)
                     /locus_tag="U2I75_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN1697050.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2821..3459)
                     /locus_tag="U2I75_001929"
     CDS             complement(2821..3459)
                     /locus_tag="U2I75_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN1697051.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3474..3773)
                     /locus_tag="U2I75_001930"
     CDS             complement(3474..3773)
                     /locus_tag="U2I75_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1697052.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(3816..5300)
                     /locus_tag="U2I75_001931"
     CDS             complement(3816..5300)
                     /locus_tag="U2I75_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1697053.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5320..5781)
                     /locus_tag="U2I75_001932"
     CDS             complement(5320..5781)
                     /locus_tag="U2I75_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1697054.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5799..6866)
                     /gene="ulaG"
                     /locus_tag="U2I75_001933"
     CDS             complement(5799..6866)
                     /gene="ulaG"
                     /locus_tag="U2I75_001933"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN1697055.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7084..7854
                     /locus_tag="U2I75_001934"
     CDS             7084..7854
                     /locus_tag="U2I75_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1697056.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8127..8954)
                     /locus_tag="U2I75_001935"
     CDS             complement(8127..8954)
                     /locus_tag="U2I75_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1697057.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTEIDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9521..10756
                     /locus_tag="U2I75_001936"
     CDS             9521..10756
                     /locus_tag="U2I75_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1697058.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11755..12015)
                     /locus_tag="U2I75_001937"
     CDS             complement(11755..12015)
                     /locus_tag="U2I75_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1697059.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12042..13343)
                     /locus_tag="U2I75_001938"
     CDS             complement(12042..13343)
                     /locus_tag="U2I75_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1697060.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(13635..14093)
                     /locus_tag="U2I75_001939"
     CDS             complement(13635..14093)
                     /locus_tag="U2I75_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1697061.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14106..15436)
                     /locus_tag="U2I75_001940"
                     /pseudo
     CDS             complement(14106..15436)
                     /locus_tag="U2I75_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(15447..16889)
                     /locus_tag="U2I75_001941"
     CDS             complement(15447..16889)
                     /locus_tag="U2I75_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1697062.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(16876..17640)
                     /locus_tag="U2I75_001942"
     CDS             complement(16876..17640)
                     /locus_tag="U2I75_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN1697063.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(17744..18619)
                     /locus_tag="U2I75_001943"
     CDS             complement(17744..18619)
                     /locus_tag="U2I75_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1697064.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(18874..18958)
                     /locus_tag="U2I75_001944"
     tRNA            complement(18874..18958)
                     /locus_tag="U2I75_001944"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18922..18924),aa:Leu,seq:caa)
     gene            complement(18976..19047)
                     /locus_tag="U2I75_001945"
     tRNA            complement(18976..19047)
                     /locus_tag="U2I75_001945"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19013..19015),aa:Gln,seq:ttg)
     gene            complement(19057..19129)
                     /locus_tag="U2I75_001946"
     tRNA            complement(19057..19129)
                     /locus_tag="U2I75_001946"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19094..19096),aa:His,seq:gtg)
     gene            complement(19191..19275)
                     /locus_tag="U2I75_001947"
     tRNA            complement(19191..19275)
                     /locus_tag="U2I75_001947"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19239..19241),aa:Leu,seq:caa)
     gene            complement(19296..19366)
                     /locus_tag="U2I75_001948"
     tRNA            complement(19296..19366)
                     /locus_tag="U2I75_001948"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19332..19334),aa:Cys,seq:gca)
     gene            complement(19412..19483)
                     /locus_tag="U2I75_001949"
     tRNA            complement(19412..19483)
                     /locus_tag="U2I75_001949"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19449..19451),aa:Gln,seq:ttg)
     gene            complement(19495..19567)
                     /locus_tag="U2I75_001950"
     tRNA            complement(19495..19567)
                     /locus_tag="U2I75_001950"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19532..19534),aa:His,seq:gtg)
     gene            complement(19570..19643)
                     /locus_tag="U2I75_001951"
     tRNA            complement(19570..19643)
                     /locus_tag="U2I75_001951"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Trp,seq:cca)
     gene            complement(19650..19730)
                     /locus_tag="U2I75_001952"
     tRNA            complement(19650..19730)
                     /locus_tag="U2I75_001952"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19694..19696),aa:Tyr,seq:gta)
     gene            complement(19732..19807)
                     /locus_tag="U2I75_001953"
     tRNA            complement(19732..19807)
                     /locus_tag="U2I75_001953"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19772..19774),aa:Phe,seq:gaa)
     gene            complement(19843..19915)
                     /locus_tag="U2I75_001954"
     tRNA            complement(19843..19915)
                     /locus_tag="U2I75_001954"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19880..19882),aa:Asp,seq:gtc)
     gene            complement(19925..19997)
                     /locus_tag="U2I75_001955"
     tRNA            complement(19925..19997)
                     /locus_tag="U2I75_001955"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19962..19964),aa:Val,seq:tac)
     gene            complement(20013..20084)
                     /locus_tag="U2I75_001956"
     tRNA            complement(20013..20084)
                     /locus_tag="U2I75_001956"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20049..20051),aa:Glu,seq:ttc)
     gene            complement(20099..20187)
                     /locus_tag="U2I75_001957"
     tRNA            complement(20099..20187)
                     /locus_tag="U2I75_001957"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20151..20153),aa:Ser,seq:gga)
BASE COUNT         6338 a         4263 c         3209 g         6414 t
ORIGIN      
        1 agacgcagtg ttgacaattg aaaaaaaata aatgtttgta taccatatga ttatttcaat
       61 atgtatcctg gaattggaga tctcgtagag ctatatcaag atgaatcaaa tatacttgtc
      121 attcatcatg aaatatagag acttataaaa ttgtttattt ttataatgta tcagtataga
      181 aaggaaataa aacatggatt atactgttga atttgacagt gaactactta agcaatttcc
      241 acaagaagaa catttcatgt ataaagaagc aatttcccac aaagtgacag agtatttgga
      301 gcataatttt tttcgtataa agccagtgcg tactcgttcg gcaaaaggaa tatacgagat
      361 gaaagtcaaa attaataaaa aaaattatcg catagcattt gctttaaaaa aaaatcatgt
      421 gttcattttt tatattagtt cgaatttgca aaaaattgta tttgataaag aggtgagtaa
      481 aaaaataaaa tagctgagca ttggaggata gattgagtga tactaagtct atcctcttgt
      541 ttttctaaag aactaagcta gaattttttc ttttgtacgt tattatatgt tatttgtcat
      601 taccaaaaag cacctttttt cttctagtat ctatgacata agaaatacca cccatttcct
      661 ttacttgaac aaatcctact gattcataaa atttttttgt tttctcagta tcatctgtca
      721 ataattcgat ttggactggt ccataactag tgatcttctc caaagcttca ttcaaaagca
      781 ctttgcctat tccttggcgc tgatacctcg gatgaatgag gatatcttga ataaatgcga
      841 tcgtcgcaaa atcagtgatc caacgaacga ttccgataat cttcccttcc ccattacgct
      901 ttatcagaga ttcagattgt tcaaatgctc tagctaacct tgcagtgtct tttgtgtaat
      961 tcgtccaacc aacgctctta tataattcag aaatctcatc gatcataagg gaatcaatat
     1021 tcattttttt cttcctttct tatacaattc tccctctaat aatacagttc tttgactatt
     1081 taatctatca catataactc tcctgtaaag attctctcga ttccttaaaa tctctctttc
     1141 cttttagaat gcatcctttt tgataaataa aaaggttgtg ataaattttc ttcacaacct
     1201 ctgtttctct ctatcaagtg ttatctttta attttctacc cttaccctag catctctatt
     1261 gcccgtagta tgcatacttt ccatgtttgc gaaaataatg tttatctagt aaatattgcg
     1321 ggatttcaca tatattgggg ttgatcagct cgttttcttt ggccatcaga caaatttcat
     1381 ctaagatcaa actgttttct actgctttca taggtgaatc cccccatgtg aacggtccat
     1441 gtccatagac aagtactcca gggacttcgt ttggatctaa ttttctttcc ttaaacgtct
     1501 cgacaatcac attccctgta tgaacttcgt aagcttctct tacttcttct ttcgttaatt
     1561 ggcgtgtaca aggaacctta ccataaaaag catcagcatg agtcgtgcca taagctggca
     1621 aatcgcgtcc tgcttgcgcc cacatgacgg agtgcgtgga atgagtatga gtaatagcag
     1681 ttatatcttc aaaagtttga tacaaaacaa catgtgtggg gagatctgaa gatggtttta
     1741 agctatcttc ttctattata tttcccgaca aatctgttac caccatttga tctgcttgca
     1801 tgcattcata tctgacaccg cttggcttaa tgacgatgac tcctagctca cggtccactt
     1861 cactcacatt gccccaagtc aatttgacta atccgtatcg cggcaatgcc agatttgctt
     1921 caaggacacg ttgcttcatt gctgcaattt tttcttcaca agacatcata gccagcctcc
     1981 ttcaatttag aacttaagta gtcttttgcc tgctggattt ctcttcgaaa gtcttggctt
     2041 ttttcactcc acatttcaat cagaaatgtt ccatcataat ctagacgttt caaatgtctt
     2101 aataatccaa gaaaatcaac acaaccgcta ccaaaaggaa catcacgaaa ctggcctgaa
     2161 tattcttttg ttacagggta agtatctttt aagtggatag cagtgatata atcgatccct
     2221 ttctcaagtt ctcgagccgg attattttcc ggccatgcgg acaaattgcc taggtctgga
     2281 tatacttgta gaaaagggga atgaatctgt tctttgattt ctaaaaattt tgaaatggag
     2341 ttcataaatg gatcatccat gatttccacg gaaagaatga tgccatattt agaagcttcc
     2401 tttacgcttt cttttattcc ttcgataaag tcttctctgg aattcattga tttctcttca
     2461 taataaacat cgtatccagc tatttggatt actttgacgc ttagattatg cgccaaatgg
     2521 attgcttttt tcattagttg tttcgctgct tttttctttt ctggatcagc agaaccaaac
     2581 ggaaatcgcc gatgcgcact taaacaaatc gaatggatac gtacaccgga gctgaacatc
     2641 tcttctctca gctcagctat cttttcttca gaccaatcta atcgtgctaa ccgctcatcc
     2701 gtctcatcaa tcgacatctc gataaaatcg aagtccattt cttttacaag ggaaaaacgt
     2761 tctttccatg aaatatcttt gggtaatgct ttttcataaa tcccaatttg agccatttcc
     2821 ttacccccag atacgaatga tctcatcttt gaagttttgt gcggccttca tcggatcatt
     2881 tgctgctgta ataccacgac cagtgataaa ggtatacaca tcgatccctt caaataagtg
     2941 taatgtgtgt ggatttaatc caccagttac agaaacatta aagcctagtt caatcagttt
     3001 tttgatcttc gataaatctt tttcacccca agtttcacca gataacagcg catcacggct
     3061 ttgatggtag attacctgcc ggataccgat gttatgccaa tccatggctt gttcaaaagt
     3121 ccaattgccg tagagttcta cttgcaattc acctatttct ttttgtgctg cctccatagt
     3181 tggaatcgtt gcacaacaaa tgactgtcat ccagtctgct cctgcttttg caacattact
     3241 cgccactgtt cctccagcgt ctgcacattt cgtatcagcg actagttttt tatctgggaa
     3301 catactgcga atacagcgaa tcgctttttg tccttcctgt aagcaaagaa ttgtgcccac
     3361 ttcaataata tctactacat caccaacttt cataacatct cctaatgcat gttctaagtc
     3421 attgtgatct aacgcaattt gcaaattcgg cctgctcatc ttagttcctt cctttacttg
     3481 tttttttggt accactttgt ttcagcaaat ttttcactga tttctttggc tgacatcaca
     3541 tttttgatac cgataactgt aacttctttt ttctctgcct gagtaaacat attcaaaaaa
     3601 ttgactggcg taaaaactac atcgtattgg ccagctgaac tttttccctc tgaaatggca
     3661 caatgatgga ttttggtaat tggtacgttt agttctttca ttgctttttc cacacttctc
     3721 atcatcatca agcttgttcc tgatccattt gcacacgata ctaaaattct catcttaatc
     3781 cctccattat gagtgttgtc taatcagatt ttcttttata ctctctgctt ttctgcatat
     3841 gtgtcatagt cttctacaat caaaaaataa ccctcaggat ttttccgata ttgtaactgc
     3901 ggaatagcca ataaaccaat gacaactacg gcaattccag cataacctag gactttcatg
     3961 attactgtaa acaatggcca agctgttgcc cagtcgaaca tcccgatata accaccatat
     4021 gttgacatcc caatccatgt agcgaataat gctgaaccgc ctacttgtat gattcctgaa
     4081 agaaacggga acaggcaagc ggctttgaat ccacctcggt tattggcata aactgctata
     4141 acggcattat caaagaacaa cggaatgaaa ccggctatga cgatcgttgg tgatttcaat
     4201 aaaatcagta aaccaatcat caaaaactgt cccaaagcac caaacaagaa cccaatcgtt
     4261 accgcatttg gcgaaccaaa gccaaaagta gcagctacgt caattcctgg aacagctcct
     4321 ggtaaaattg tgtttgagat tccttggaaa gattgagtta gttcatcgac gaatgtccga
     4381 acacctaatt gcaagatcgc cagatatacc gcaaaattta aagaagtcgt cataatgtag
     4441 aataagaaac tctgtccttc ttgcatgaat tgcgcttgaa tcaaataatc tttgcctagt
     4501 acaatcagaa tgatcccaaa gaaaaatagc atgagtaacg aagtagcaac catattctca
     4561 ttgaaaatcg acaagaatcc tggtaatttg atatcttcga tttttttgtc ttgcttgatt
     4621 gatctatttt tcttttcatc ccgctttttc atccactcag ctaattttgc aaatataaag
     4681 attccgaaca tttgctgatg tgccactgca aatccggcac cttctgtcaa ttcctgcgtg
     4741 attcctactg ttaagttaga agctactgcc caataacaac ctaaaatcag tcccataaaa
     4801 atcaatactt gtatcctacc tagatcagga aagcaaaaca gtaaaatcca aaaagcggta
     4861 gctgcttgtt gaacctgaac gtttccggta gtgaaaacgg aacgcagttt cgtatatttc
     4921 tgaaaacgga ctaataagat atttaaaaca aatgcaatca gtaataaaat catcacatct
     4981 ccaaaggtac ggccaaatgt ctccattaat ccagcatcca cagcattttg tccaaaataa
     5041 ggatctgtaa ccatggcatc caaattgaac cgttccttta gtccaaccaa aatcggccga
     5101 aaattattga ccaaaccacc agagccaaca gtcaaaataa agtaaccgac cgttgccttt
     5161 aagaatccag cgatgcattc atgaagcggg cgtttcagca aaatatagcc taataacacg
     5221 ataaaaccaa tcaggtatgc tggctgcgtc aagatatttg ttgcaaaata cgtccaaatg
     5281 cttaacaaaa attcccccat cttttttctc ctctatcaat taattatatt ctgccatcac
     5341 aacttggtaa tcatcgatgg agttcgtaga gatgagacgc tcaaccaatc catctgtcat
     5401 taataattcg gataattttt ggatattttc tagatgttct tctggatctt ttgctgcaag
     5461 tgtaaaaaac aaactagccg ttttctcctc atcctcccct tcactttcaa aacggattgg
     5521 ctgtttcaac ttggtaaatg aaatagctgt tccaaaaaca ccatcacttt tttctgaaga
     5581 atgcggcatt gctacttgag gtgcgatcac aatataagga ccgtattttt ctacacaatg
     5641 gatgatctcc tccacatacg tttcatcaat aatttcctta tcgatcaata caagacagct
     5701 ttcggcgata gcttcacgcc aatccgtgat ttctttatct gggtaatgaa tcaattgatg
     5761 gtcaaaaaag tatttcagca agaaactccc cttctttctt ataaaaacga cggaaacggt
     5821 gtatcatttt caatcgagaa gacatcttca aatcctcgat aaaaattaaa ctcaagatca
     5881 tctttgtttg tcggatagat gaatttcccg cctacttgcc agatataagg tttaaatcca
     5941 tacttcaggc gatccttttt gaatttccaa atttcagtga tctcttttga atcagccata
     6001 aaattagacc aaatatcgta gtggacagga atcactactt ttgcattcaa tgactccgcc
     6061 atgcgcagca tatcaacaga agtaactttg tctgttatcc ctcgtgggtt ttcaccatag
     6121 gcccctaaac agacatcgat tttgtgttca ttaccatgtt tggcaaataa attggagtaa
     6181 tgtgaatctc ctgcatgata aagattccct cctgatgttt cgaataaata gtttactgca
     6241 atttgatcca tctcctgagg catttttcct tttaatacct cttctggatc ttcacaagtg
     6301 accaacgctg tccgatcaaa cgcttctaaa gctactacat tgatgtcttt gattttgact
     6361 gcatcacctg gctttactgt aatcgttttt tcttcaggaa ttccccattt ttgccacgtg
     6421 cgaaccactt cttttggccc aatgaatgtt gcttcaggac aattttgatg aactgctgcg
     6481 gctgtatgga tatctagatg atcggaatgg atatgtgtaa cgattaaagc gtctacattt
     6541 tttatcgcaa aaggatcgat tacgaaaggc tgagtacgta aattaggctg catattttga
     6601 acgccactca tacgcatcat ctgatggcct tttttcatca tgccattccc atgactacgt
     6661 tttcccgtcc cacaccatag atcacataaa atattcgttc cttcatgact tttcagccaa
     6721 attcccgtac agcccagcca ccacatagca aaagttccag gatccactac ttctgtttcg
     6781 atttcttcat tcaagtatgt gccccactca ggaaatgttt ctactaacca cttttctttt
     6841 gttacttcat tgacagtttt actcatttgc ttgctccccc catatcgttg ttccaactta
     6901 caagtaaata ttaatgtgta tcagcatcca tggaaaccct tttatatgac cgtttgtttt
     6961 ttatatgatt ttctttcagt aatcaatgat tgtttcagta taataaagac agatatgaat
     7021 gtttaaatga acaactagtc aaataaatcc atggatcaag atgtccctca ggaggcaaaa
     7081 gtcatgaaaa actcacttgt caatattgaa aaacgtcaaa aagaaatttt ggaactgctt
     7141 cagcagactg aacaactatc aactttcgaa ttagctgaat cccttgatgt atctattagt
     7201 acgatacgaa gagatttaaa tttattagaa gaaaaaaacg agatcatacg caattacggt
     7261 tattgttctt ataattacaa aaaccagact gattttgatc aatctggtcc tgaaaggatt
     7321 aaacaagcga ttgccagaga agctagtaaa tacattaacg attatgatac gctgtttatc
     7381 aattctagct cgactgcgtt gaaaacactg gattttcttc aatctaacca tttgacagtc
     7441 attacaaaca acgtgaaaat caattactct ccgcacaaag ataattgtag ttatatttta
     7501 actggtggag agatgcgctt tccaaaagaa gcactagtgg gcgacatcgc attaaatacg
     7561 atttcttcag tcaatgcaga catttgtatc attggctgta gcggagtcga tgtagtacat
     7621 ggggtcacaa caaagatatt aaatgaatca acgatcaacg aaatgatggt caacaagaca
     7681 atcaaatgta agattttagt cgctgatcat cgaaaaatcg gactgacatc aaagttcaag
     7741 attgctgata tttcagtctt tgattatttg atcactgata aattttgttc tgaaaaagta
     7801 ttagaggaga tcagaaatac ggggataaaa accattcaag tagattcatt ttaagaacca
     7861 ggacgtaaat atgtaaatag gattatcaaa aaaatgaata acaaatttat cgataaatga
     7921 aaaacacgct acgctcaaat tgataaagga atcccaagat gtgtccatcc aatcgatacc
     7981 atcataatct attgcttctt ttataaccgt tatatgttgc ctaaatagga atcaaacaat
     8041 atcgaaaaaa ggaggtatga caaaagtcac acctcctttt ttcgaataaa cggtattcaa
     8101 atcagaacac tgatttcaca accactttat ttcaggtcgt tttcaatcac ctttaccgcg
     8161 taatcatttc cgttgaaacg cgctattttt tttgcgtcca tatatctttc taaataatca
     8221 tcttctccta attgcttatg gcaagaagca atttcataaa gcaagtattc caatgcataa
     8281 gtcgatttat gtttcttatt atactcaata cccttctcac ataattggat tgctttatca
     8341 taatcctcga ttttacggta ataggtggcg atatcataat atacaatgca gaccatcaga
     8401 ttatcaaaat cagtctcact caatatttta agtgtacgct ccacataaac tttcgcaaaa
     8461 tctaattctc cttttgacaa gtagatcatt gccacgtttg cagctgcttg taagtgatgg
     8521 atatctattt cggtcgtttg ttccaacact tgattaaaat aaaataatgc tgcatctggt
     8581 tttttcgcat aaagatgatc aagcagtcct tgataaaata aaatttgatt taaaagatca
     8641 gaattactta ctttttcctg atcgatcgtt tgtaaaattt tataagattc ttggtatttt
     8701 gatacagaat tcaatgattg aatctcattt atttttttcc tgattttgtc ttcttctgtt
     8761 tctactaata tatcggagat ctcgacattc agctttttgc aaagcgcact taagatattg
     8821 atgcctggcg acatattctt gttttcgata ttgctgacag ttgcttgttc acaaatgcct
     8881 tctgctaatt gtacttgtgt taaccccttt ttctttcggt attcttttag taatgctcca
     8941 tctactttaa tcaaaacaat ttccttccct ttaaaaaatt atttctcctt tatatcatac
     9001 acaaaaaaaa gttctaaaca accaaaaatg tttagaacag aataaaaatt ttaattttaa
     9061 aataatattc aaattaaaca actggcgtat ctgctccttg atttcttttt gtgttatctg
     9121 aataattatc gtttacagtt ataacacata gtgaaaattg tcatggcgag aaagtctaag
     9181 aatattttta aacataacta tttctttctt acgtgtgtat aatgacgaca gcacacctat
     9241 caattaatat aattatttta ttttttatgt tctcatgttt atttttaatg acacaacttc
     9301 tcgtatttat ctggcacaac atataaatcg atcaaaaaat tgttgcgtgt ttttttgaaa
     9361 attggagttt caacttgatt acaaaattta aatttagaag ttgtgaaaac agcgagagac
     9421 agtttaatat tcaaaatata cataggctac tcgaacgctg tgttttttag tatactagta
     9481 agaaacgaag ggatagactc attccaataa ggaggatttc atgaagacaa ttctttttgc
     9541 tcctgcagta ttcaatttag ctgagacaac acgaatgatt gaggttgcca aagctatgaa
     9601 agaccgttat tactgtgtat tctttggctt ttctaaaact tttgctcctt tgatcacaga
     9661 aagtggcttt gagtatcatt tactagcgcc gatccttacg aaaaaacaag aagaacaaat
     9721 cataaaattt gaccagttgc gtacacttaa aaaccctttt accaaagaaa tggttgcctc
     9781 tcgtatcact agtgaacaga gtctgattgc taagtttgag cctcgactga ttgtgacagg
     9841 ttcgaatgtg acaatttttt tatcggcccg caataaaaag attcctttag tatatgttaa
     9901 accctatgct ttgagtgaac ctttttttca atcagagcat gcgctgatgc cccacgagtt
     9961 acgtttcttc ggccctttaa gcaatcccct ttggcactgg acaaaaaagc gtatagtaaa
    10021 tacccgatgg atccccaatg ccttcaaaca cgctgcacaa aaagaaatgc ttcctttacc
    10081 aaaaacaagt ttacaaatga tggatgctga tctaaatctc atcacaacgc ctaaactatt
    10141 tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt ggtccaattt ttgctaagct
    10201 cagtacttcc ttgcctgaag aagtgctaac ttttatacag cgaaagcaac aagaaagaaa
    10261 acgtattatc tatttcgcaa tgggtagttc aggcaatccg cgtatgttac gcaaaatttt
    10321 gttcttctta caaaaacaac cggatctagc ggttattgca cctattgact atctgcttga
    10381 tgacattcaa tctgactcag aagatctttt aataacagaa tatctgtcta gtcatctttt
    10441 gcatgaattc attgattttt cttttatcca cggcggggaa gggaccgtcc aaactgcctg
    10501 tgaatccggc aaaccgttta tcggtgtggg gatgcaattt gagcaaagct gtaatattga
    10561 ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa ccagtgactg aaagaaaact
    10621 gcagattgct ctgcgcgaag tttcttcacc caagattcgt cagcaggcac gcacgctgca
    10681 agccgttttt ccaataaatg gtccacaaca agcaattcaa gaaatcgaaa ccttcctgag
    10741 caaccaccca atatagtttc aagtagaaaa agctttttta atagagactc atcagtaatt
    10801 tcgctattcg agacccagac agtgaaaata caaaaaaagt aaatagtgga ttgaaaagtt
    10861 agagttactt aaattacggt gtctaaaaaa cacaaaatca tccttaaaat aaaagatctg
    10921 acgcaatcca aatgagcata ggtagcgtct gccatttatt cttagtcaaa ttctttgaca
    10981 cctttctctg gttggatcat ctcaacgttt cttcaaaaga aagaccataa taattaggca
    11041 aatacatttt cttaaaatgt gtcatataga tctagaagca tcgctttgta gagatttgtg
    11101 ctgctagtcc cttcaacaat tactaaatca aatgtaggat ttaccacttt atctttgata
    11161 atcaacctcc ctcttctgtt gagacaatga tctcgcaatc cgatgcatac tcctatataa
    11221 actgccagca atccccacat gctgagtaga tcatgtcaga atgttctatg tcgtcagcaa
    11281 cgataaacta aaagtcacgt tcaccatgac tgattgctat tcccagcgca attatttcta
    11341 tacacgtttc atgatttgaa taaacactct catcatacat attaccattc atacaccata
    11401 ctgcagcccc tactatatgg ttgtaagctc ccaaatctat atttgccttg atcactttgg
    11461 tagtagatcg actaattctt aatccattgc tgataaaatt ttcatttttg cctccttctt
    11521 tctgccaaaa cagttaaaac aataataatc tttatgtaaa aaagggctgt gacaaaagcc
    11581 ttgtcacagt cccatattcc gaataaacgg tggtcaaaag cttgcatctg cggtaataag
    11641 ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt taagaagcaa ttttcgtaaa
    11701 tttcttctta tttcttgaag ctaaccactt ctgtcacaac cacttttaga acatttaaag
    11761 cttcgatgtg acaaaccatc tatgctgctc gatcataaaa ttgatagcaa aaattccaca
    11821 aagtacatat gtcccccatg aaaacaaaac attcatatgc ggtacaatga tctttggaag
    11881 aatcacgatc aaaaatgatg ccagtgccca aatccatcga ttgatttgaa tatgatatgt
    11941 agctagcata ttcaatataa ggtgactagc cgttaataaa aagataaaaa caaaaaataa
    12001 aatcagccaa tacattgttt cccaccttct aaaaaccaaa cttagacagt ttggacttcg
    12061 ttttctccac gactttgttc atctgacagc atctttttat cataagcttt tacaaatgga
    12121 taatagataa taaatgctat caatgtgttg atcatgatca agataacaac acgccagtca
    12181 tttcctgctg aaagataacc gccgattgga gctggtaatg tccaagttgg caaaacagta
    12241 aatctattga ccatttctaa tttgactgca aaataactga taatagtcaa aactaaagga
    12301 actaagttga acgggatgaa catgtaagga ttcaacatca taggagctcc aaaaatcatg
    12361 ggctcattga tattgaagat accaggaata atagaaaaac gaccaacctc tttcaagaat
    12421 tttgatttac agaagaatat aaacaaaaca gataccacga tcgttgctcc tgcaccacca
    12481 attgttaccg tccattgata aaactgttca ggagcgatat agggtaattg ggtggcagct
    12541 gtaccatctg ccaatgcctt tgcattctca tccaacatga tcagccacat tggacgaaca
    12601 acagccccta caatactcat gccatggata ccaaatgacc acaacaaatg gatcaaaaac
    12661 attggtagta aaacaccaag caaattattc cctaaaatat tggttaaagg ctcgaataac
    12721 gataataagg tagcgtttac atcaaagccc gcgacgcttc gaatcagcca agtaattgtt
    12781 aaaatgactg cacctggaat caatgcctca aaactacggg caacagcagg tggcacttgt
    12841 tctggcattt tgatagtaat atttcgattt ttaaagaaac gatatacttc cactgcaaaa
    12901 atcatagata agatccccgc aaacatccca gatgctccca aagaactcat aggtaagacc
    12961 cagccaagag gtgccttttc aggagttaaa acgtttacag gaacttgtaa cataaagaac
    13021 gttcctaaag ataatatacc acctgtcaca ctatctaatc catatgattt agctagatcc
    13081 gaacccataa caaaagttgc ataaatagcc atcaatccca tcgtcatcct aaaaggtaaa
    13141 acaatcgttg ccgaataagg ggcaataaat tcattccatc caggaaacgg taaattgata
    13201 aagaccataa aaaatgtccc aatcaaaatc aatgacaatg ttgaaacaac cccattacga
    13261 attgctaata aatgacgctg tgtaccaatt tttgccattg gtgggacaag tttctcttcg
    13321 acccatttca tcagcttttc cactaaaacc actccttttc aataaaatat gaaccattca
    13381 ttgcatgaca acgcaaagaa tgttcataac taattatata attaagaaat tttatttcaa
    13441 tatattctct taaataatca aatttatttt taagaatcct gaaaataagt tttctaaatc
    13501 ataggagcaa caaaaaaatt cgataattaa caaaaatcaa aatgattaaa tctagtgatt
    13561 gaaacttatc atttcaaaaa aattttgtaa attcacccat aaatatcacc ctatttaact
    13621 tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa tctttacttc ttggttttgt
    13681 tttaataaat ttccttcaaa aatgcctgga gaaactacaa tgatcaccgt atctttttgt
    13741 gcttgctcca acttcttcaa atctataata gctaacagtg tcccttcttt tacctcctgg
    13801 ttactttcaa cccgtatctg aaacggactt ccttcgagtt caactgtatc taaacccata
    13861 tggatcagta tttttctgcc agattcactt tcgatcgtta tggcatgtcg ggtaggaaat
    13921 atattatcga ttattccttt aactggcgca tggatatatc catcatgatt cgttatagcg
    13981 tatccaactc ctatcatttt tgaagaaaag acctcgtctt ttactgctgt taatggaata
    14041 atttgaccac tagctacagc ttttagaatg tttcttgttt taaagaaatg cactttttat
    14101 cacctctact ttaatgctgg ccaatatttt ttgtttactt tgatcatttc atctagtatt
    14161 ttttttgcga ctctggcatt tggtacggtt tgattcaatg taaatgcttg taaagctttt
    14221 tgataagaac cttcaaagta agcatctact agtaacttct ctgctgcaac ttgtgcctcc
    14281 atcaatcctt tatgaaaatc actgattggt tttcttagtg taattgtctc cacaccagtt
    14341 gcaccaacat atgccggtat ttcaacaact gcatctgacc gtagattcgg aatcgcccct
    14401 tgattaggaa cgattagcat aaagcgacga cgatcatcat tcaacaagga aatagctata
    14461 tcgacgatat attgtccatg ctccccaaaa ttaaagttca atacttcttc cacaataccg
    14521 cggcgtattt tatcagccat ttctttggta tttttctctc gtccatccat gaccatatta
    14581 gcacgtgtat actctttgtc agtatgctca acgaccatat caggatataa gtaatactct
    14641 aagtaattat tcgggatatt acttggaaaa tttttcgtga tgactgacat catttgaaag
    14701 gttttttgcc aagttttgtc tcctatatta aaatcggcga cttgcatttc ttttgttatt
    14761 agtttttcaa taatctctgg cataatttct cgttgcaatt ctttatcata aatcgatgta
    14821 taccagccaa agtggtttaa accataatac gtaacgatcc aattcttacg atcataaccg
    14881 taattcactg caatcgtttc ttcaattgaa atagtcatat cacaggcatt aatcattttt
    14941 acattcggat atttacgacg aacagcttca gaaacgatcg actctggatt tgtataattc
    15001 aaaatccatg ctttgggggc atagtcttgt atatgatcga caatttccaa taaacctttg
    15061 attgagcgca tcccataagc aaatcctccg agtccacacg tttcctgtcc tactaatcca
    15121 tgtttaagtg gaatcttttc gtccttttct cgcatctcca atcctccaac acgaatttgt
    15181 gagaaaacaa aatcgcatcc tgtaaaagca agctttggat catccgttgc acgaatgcga
    15241 atatttgaaa aacctttttt ttcagcataa atttaacaat caagtacata tccatatttc
    15301 tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt tcattgttca
    15361 aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata gtgataatct
    15421 gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa ttctgccata
    15481 acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg tttaatgatc
    15541 ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc cactacctct
    15601 acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc tccaaacgct
    15661 tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa ctctgtttct
    15721 tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt
    15781 ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa aattccgatt
    15841 ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc aacaatacat
    15901 gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc tgctaaaccg
    15961 gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact aaattcaaca
    16021 ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc
    16081 atctctttat tttctttata agctgcttta tagatagcta gacatatagc tggtatacca
    16141 aacatcatta ctacaaaaaa acctgataaa aacagtccag cctgaggatc tccgttgata
    16201 aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct tcctaaatcg
    16261 taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa acgattcaaa
    16321 aacataaata tccccacacc taatgccccc atatcaacga gcagttttgc aaaattatca
    16381 atcccaattt ggatgagcgg ccagataaag cccaaaaaaa tagctgcgat tgtttgtaga
    16441 atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac tactggtaat
    16501 ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat aattccacca
    16561 aaaatcccca tattcacttc tgggtccatg atttctaagc cttttttcaa ggtagcaatc
    16621 gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa accgattgca
    16681 actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc tgcaccaatt
    16741 actgggatgt tcagcatatc atcagctgat aaacgcatga tcaatcccgc taatggcatc
    16801 gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt aacattcatt
    16861 tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag gatcattttt
    16921 cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgagtac gaggatacac
    16981 aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca tgacttcgat
    17041 cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca cactcgtatt
    17101 gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct caaaataatc
    17161 gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat catatttttt
    17221 tgctaatgta acaactacct cctggttttc atccaaagta tgagtatgat gatgactgtc
    17281 taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat tccattcctg
    17341 atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg atctgaactc
    17401 tccgctttcg ataagagtat cgacagtttt gagaagtggt ttgccacacg tcaatgttaa
    17461 atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcgt gctcgaaacc
    17521 cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag catccataat
    17581 cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca cttttcccat
    17641 ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata gtccatttac
    17701 tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag attcgtgcta
    17761 gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat tgctgttcga
    17821 tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat gtttcagtaa
    17881 aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta accaggaatt
    17941 tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg tttgaatagg
    18001 atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag ctctttaaac
    18061 tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct acttcatttg
    18121 accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctatt gctatatcat
    18181 attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca tctctatctt
    18241 ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag atatttgaat
    18301 ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca aaatccataa
    18361 attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct ttagaatcta
    18421 atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta gcagaaaagc
    18481 caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat acagcattat
    18541 gattcagctt tttcatcaca tacaccgtta attcacctcc actatatgat aaaacaaaac
    18601 aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag acaaaaaacg
    18661 acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc ctacataatt
    18721 tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac tcgttttatg
    18781 ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa aatatagcaa
    18841 ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc gaaccggcac
    18901 gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc acgccagcaa
    18961 atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc
    19021 cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga atcgaaccca
    19081 cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc atcataaaag
    19141 atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa taccggcggc
    19201 cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg cgtctgccaa
    19261 ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat ttgaaccggt
    19321 gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta tactattatc
    19381 aatctacaaa ataaagaata aattatagaa actggggtag ctggattcga accaacgcat
    19441 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa aaagggcgaa
    19501 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
    19561 cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg gagaccgctg
    19621 ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac cactgaaccc
    19681 taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc atggtggcgc
    19741 gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc aactgagcta
    19801 ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg acgggaatcg
    19861 aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg gaaccagttc
    19921 attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc
    19981 agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg aacccgtgtt
    20041 accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg
    20101 gagagtaagg gattcgaacc cttgagacag tttgcaccat ctacatgatt tccaatcatg
    20161 ctccttcggc ctctcggaca actctccaga gtgaaagaag ctcaatactc tttctcaaaa
    20221 tgag
//
LOCUS       DASHFF010000049        18965 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.049, whole genome shotgun sequence.
ACCESSION   DASHFF010000049 DASHFF010000000
VERSION     DASHFF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.049"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(20..250)
                     /locus_tag="U2I75_001958"
     CDS             complement(20..250)
                     /locus_tag="U2I75_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697065.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(686..1252)
                     /locus_tag="U2I75_001959"
     CDS             complement(686..1252)
                     /locus_tag="U2I75_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1697066.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1252..2208)
                     /locus_tag="U2I75_001960"
     CDS             complement(1252..2208)
                     /locus_tag="U2I75_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN1697067.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2208..2864)
                     /locus_tag="U2I75_001961"
     CDS             complement(2208..2864)
                     /locus_tag="U2I75_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN1697068.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3234..5252
                     /locus_tag="U2I75_001962"
     CDS             3234..5252
                     /locus_tag="U2I75_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1697069.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5322..5528
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5679..8093
                     /locus_tag="U2I75_001963"
     CDS             5679..8093
                     /locus_tag="U2I75_001963"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN1697070.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8441..9994
                     /locus_tag="U2I75_001964"
     CDS             8441..9994
                     /locus_tag="U2I75_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1697071.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10375..12159)
                     /locus_tag="U2I75_001965"
     CDS             complement(10375..12159)
                     /locus_tag="U2I75_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1697072.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12174..13895)
                     /locus_tag="U2I75_001966"
     CDS             complement(12174..13895)
                     /locus_tag="U2I75_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1697073.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13995..14756
                     /locus_tag="U2I75_001967"
     CDS             13995..14756
                     /locus_tag="U2I75_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1697074.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15723..15991
                     /locus_tag="U2I75_001968"
                     /pseudo
     CDS             15723..15991
                     /locus_tag="U2I75_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16663..>18965
                     /locus_tag="U2I75_001969"
     CDS             16663..>18965
                     /locus_tag="U2I75_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697075.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT         6231 a         3364 c         3805 g         5565 t
ORIGIN      
        1 tcattaaaaa gcataaccat tactccgatc tgtttttaaa attagtttct acctctaata
       61 tatatcgagt ttttattttg ccttcagaga atactgtttc tttttttgca gttacaggtt
      121 gtttattttc ggaaaaagct aatatagcta agattgaaac atccatctgg aatttatctt
      181 ttttactgct ttgatcataa aagtctgcat attcatcact gatatttttt ctaataaatt
      241 cttccatcat aaaaatcacc tcaaaacgat tatacgaact tacgttcttg ttgtaaagcg
      301 atatttgagg ggcaaaaaag gggcataatt tgactgatat taagttagct attgatatat
      361 gaatgacata caaaaatata aaatctttta aaaatgctta tatatcaatg tttttgccat
      421 tcttttatat atctgttata tgtcaaaaaa atgtgttcaa tttcaatttg tatttgaata
      481 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaatc aaaaacagct
      541 tctcatgttt ttgatttggt tggacttatt accgcagata taagcttttg agcaccgtga
      601 ataaagaggt tatgaaatca acgctctaac ctgagtatta gagaaaaaaa cgcaaaacat
      661 atcatttatg ttttgcgttt tttatttatc tttttttctc aatacaatat aaaataggcg
      721 gctggttctt ttggttgata aaccgataat tcagcacatt atatgtttcc tgtggcagtt
      781 tggaacaaaa agaatcaacc atatccagtt ctttttctcc accttcatga ccatagtaga
      841 cgacaaggat caatcgtccg cgaggcacaa gtcgtttcaa tatttcttcc atagctgtgc
      901 gtgtagtctc tggcatagtg atgattgctt tatcactttt tgggagatac cctagattga
      961 aaatccctgc tttgagattt tgttgttcag ctatcacttc tcctaacgtt tcgtggcctt
     1021 gtagaaacaa tgagacacgt tcttctaact gacgctgttc tagcctttca cgagtagcat
     1081 cgattgcttg ttgctggata tcaaaactat agacatgacc agtttttccg atgtgttctg
     1141 ctagaaaaac cgtatcgtgg ccattgccca tcgttgcatc tacgacgtga tcccctggtt
     1201 gcaagatttc ttgcagtaat gtatggctga aatgcaaagc agtttgcaac atcagacgct
     1261 cacctttttc cgtacatcat attttccttg gaagctatct ctgcgaagaa gttcttcatc
     1321 aattgcattc aagacttccc attttttcaa gctccacatt ggtccaatca aggaatccca
     1381 cggcgcatcc cctgtcaaac gatggatgat gatctctttt ggtatcattt ccagctggtc
     1441 gcaaatgaca ttgacatagt caggacggct cattaattga aggcgacctt catgatagtc
     1501 ccgcaacatt ctcgttttgc gcatcagatg catcaaatgg agcttgattc cttggatatc
     1561 tgaatctaaa atggtgcgtc tgacattttc tttcatcatt tctaaacttt cgcctggtaa
     1621 tccgttgatc aaatgcgtac aaacacggat attatgtttg cgtaatttag ccacgccatc
     1681 taaataagtt tgataatcat gtgctcggtt gatcagtttt gaagtttctt caaaagttgt
     1741 ttgcaatcca agttctaccc ataaatacat ccgctcgttc agttcagcaa ggtaatcaac
     1801 tacttcatct ggcagacaat ccggtcgtgt accgactgat aatcctacga cacctggtaa
     1861 attgactact tgctcaaatc gttctttgat tatttcaact ggcgcatggg tgtttgtaaa
     1921 attttggaaa taaacgatgt attgttggac atgaggccat ttttgatgca tcatgtcgat
     1981 ttctttctgg aattggattg gaagagggtc ttcaggtgca acgatcatgt ctcccgaacc
     2041 agatacactg caaaaagtac aaccgccatg ggcaacagtc ccatctcgat tcggacagtc
     2101 aaatcctcca tcgatcggca ccttgaaaat tttcccgcca aactcatttc gtaaagcgta
     2161 attccaagaa tgatatcttt tatttccttc tgaataagga aaagtcatta aaagtccttc
     2221 tttctgtaag agttatctat ttgttttctt ttttctctat acactggata agtcaggcac
     2281 agccaagaaa gactgagtaa gtagccgccg atgatatcag taggaaaatg aacgcccaga
     2341 taaatgcggc ttatcccgac acttactata ataaacatca tcagcaattg aagcaatgtg
     2401 cgtaaggttc ttttttgaac aaaagctgga agcagaaata gtaaagaacc gtaaagaatc
     2461 atactgcctg ctgcatgtcc actagggaaa ctgtaacttg tttcaactac taagtgcgcc
     2521 aatgaaggac gctctcgcat gaaaaatagt ttgatcaccg ggttgatgat tccagcaatt
     2581 acaataaatc cagaaacgaa ccaaaatgtt tctgcccttt ttttcttata ccacatccat
     2641 aaagccgcac aagcaaataa aatgatgacg gtaagaggat taccgaattt agtcataaag
     2701 agttggtagc tgttcagatc aggataagaa gctctgacaa tgcctgtaaa ccactgatcg
     2761 aaaccagaaa gccacgacag atggaacttt acgatccagc ccaaaatgaa aaacaggaca
     2821 agaaagaagc taccagccac ctgaaatgat gttttatatt tcatacaatt cctcctttga
     2881 agcaatgact tattatagca tttttggtcc agcgaaaaaa acggtaaaga gataaaacca
     2941 atgtatgatt cctctttgaa taaccaatat tcttataaaa aaaataactg taatcggaaa
     3001 gacatttttt ggtaatgcaa atgaaatgtt agggaaataa aatcagtgtt tttcgaaatc
     3061 cttttaaaca agaacgtaga cgtaggaaga acttgaaaat gaacagaagt tgttcattac
     3121 ggtaaattac aatatgatta caaaaaacaa caaagctctt aaataacttt taataaatat
     3181 taagagaagt tataattatt aataatagat aaattgaagt tggaggatag aagatggaaa
     3241 acattgcaag aaaagaacgg agacgtttag aacagaccaa acgttttcga aaagcaaaac
     3301 gaggggcagc aatcgtggga actgcgatgg taggctgttc tgtcgctgcc ccattagtac
     3361 agccggttcc tgttcaagcg gatgaaacac ctgttcagtt cggcgcaaga atcaatacag
     3421 cggcatttat cacagaaatt gctacttatg ctcaaccaat cgctcaagcc aacgatctat
     3481 atgcatcagt gatgatcgca caagcagttg tagaaagcgg ttggggcagt agtgcgttat
     3541 ctcaagcacc ttattataat ctttttggga taaaaggcag ctatcaagga caaaccgtct
     3601 acatggatac gcttgaattt ttaaataatc aatgggtaaa taaaaaggag cctttccgac
     3661 aatatccttc ttttgctgaa tctttcaatg acaatgccta tgtacttaga aatacttcat
     3721 ttggcaacgg ttattactat gccggtactt ggaagagcaa cacgaaatcc tatacagatg
     3781 cgacagcttg tttgacaggg cgttatgcga ctgatcctgg ttacgcaggc aaattaaata
     3841 atatcatcgc aacttacgga ttgacccaat atgatacacc agctagcgga aacgcaggcg
     3901 gtggagcgac tacaggtggc aataatactg gaagtgcagg tagtactggc ggttcaagca
     3961 attctggtaa tacaaactca ggttcagcaa cttcgaccac ttatactgtc caatctggtg
     4021 attctgtttg gggaatctca aataaattcg gtatcacaat ggatcaattg atccaatgga
     4081 acaacatcca aaacaatttc atttatccag gacaaaaact gacaatcaaa ggtagccagg
     4141 aaaatggctc ttcgacgaat aattccggta acaataccaa ctcttcagga aatgctggta
     4201 catcaaacgg aggacaaaca actggtgcga aatacactgt tcaatctggt gattctgttt
     4261 ggaagatctc aaatgatcac ggaattacga tggctcaatt gattgaatgg aacaatatca
     4321 agaacaattt cgtttatcca ggacaacagc taatcgtatc aaatggcggt tcttctaatg
     4381 ctggagcaaa tactggctca aatactggga atacaagtgg ctctagttcg tccagctcaa
     4441 gttcttctaa tacagcttca ggaacgaaat atacagttaa agccggagac tctgtttgga
     4501 gcgtctcaaa taaatacgga attacgatgg ctcagctaat ccaatggaac aatatccaaa
     4561 ataactttat ctacccagga caacaactga ctgtttcaaa cagtggatca acaaacagca
     4621 cgtcaggtaa tacaggttcg tcaaccaata caaataacag cagttcagtt aatactggca
     4681 gcagttcgtc tcaaggcact tataccgtga aagcaggtga atctgtttgg agcgtggcaa
     4741 ataaaaacgg catcacgatg gatcagttaa tcgaatggaa taatatcaaa aacaacttca
     4801 tttatccagg acagcaattg atcgttaaag gtggaacttc ggcaaataca aatacaggtt
     4861 caactacttc tgcagcgaaa ccaaatacac cgaatacgtc tgctacgaca agtacatcat
     4921 ccactggtaa tacgatgtat accgtgaaag cgggcgagtc agtttggagc gtagcgaaca
     4981 aacatcatat cacgatggat cagttaatcg aatggaataa tatcaaaaat aatttcatct
     5041 atccaggaca gcaagtgatt gtgaagaaag gttcatctca atcaacaaac caacaatcag
     5101 ttactggttc gaaaacctat actgtgaaat ctggagaatc tgtttggagt gttgctgaca
     5161 gtcatggaat tacaatggct caactaatcg aatggaacaa tattaagaac aactttattt
     5221 atccaggtca aacgttgatt gtagctaaat aaaatagctg ttcgaagaaa taacgattga
     5281 attccacaaa aatatttgtt actataaggc tacttaaata tgacaacact tgaaaaggaa
     5341 gtagtaaaaa gactgaagtt tcagagagcg catggctggt gagaatgtgc acggatgctt
     5401 ttgaactcgc ctttgagttg cccttgctga atacccgagt aagcaagcgg cggtgacggc
     5461 cgttacaacg ttcgaggcct tttcactaag gtaaaattag gtggtaccgc gtagattacg
     5521 tcctataaaa agagcaatct ttttatagga tttttttatt tcttttgtga agtttgagaa
     5581 aacaaagcaa aaggaaataa aaataaacag attctgccgg ttttttcgtt atatttataa
     5641 tagaaacaag tgttttcaat agaacaggag gacagcaaat gagttacaat cataaagaaa
     5701 ttgagaaaaa atggcaaaaa tactgggcaa aaacgaatac tttcaacacc catgatgatc
     5761 ctgaaaagcc aaaattttac gcactggata tgtttcccta tccatccggt caagggctac
     5821 atgtagggca tcctgaaggt tatacggcaa cggatatcct ttcacgtgtg aaaagaagtc
     5881 aaggatacaa cgtgctgcat ccaatggggt gggatgcatt cggtctgcct gctgaacaat
     5941 atgcgctaga tacaggaaac gatccagctg agtttaccca aaaaaatatc gaaacattcc
     6001 gacgccaaat caattcttta ggatttagtt atgactggaa tcgcgaagtg aatacaactg
     6061 atccggagta ttataaatgg acacaatgga tctttacgaa gctttatgaa aaaggtctag
     6121 cttatgaagc agaagtagcg gtaaactggg tacctgaatt aggaacagtt atttcaaatg
     6181 aagaagtgat cgatgggaaa agtgaacgtg gtggctatga tgtagtcaga aaaccaatgc
     6241 gccaatggat gctgaaaatc actgcttatg ctgatcgctt attggatgat ctagaattag
     6301 ttgattggcc agaaagcatt aaagaaatgc aaagaaattg gatcggtcgt tcagtaggag
     6361 caaatattga atttaaagtg gctggtacgg ataaatccta taccgtattt acgacacgac
     6421 cagatactct attcggtgca acttactcgg ttcttgcacc tgaactagat cttgttagag
     6481 aaattacgac ccctgaacaa aaagctgctg tagaagctta tatcgaagag acagctaaaa
     6541 aatcagatct aaagagaacg gatcttgcaa aagaaaaaac aggagtattt actggtgctt
     6601 atgcaatcaa tccagttaat ggcaaggaaa tcccaatctg gatcgccgat tatgtattat
     6661 cttcatacgg cacaggagcc atcatggctg ttccagcaca tgatgaacgt gactttgaat
     6721 tcgctcaaac tttcggacta gaaattcttc cagttatcga aggtggagat gttgaaaaag
     6781 ctgcttacac agaagatggt acgcacatca actctgaatt tctagacggc atgaacaaac
     6841 aagaagcaat cgataaaatg aatgaatggc tagaagaaaa tggcgtaggg aaaaaagaag
     6901 tcagctatcg cttacgtgac tggctattct cccgtcaacg ttactggggt gaaccaatcc
     6961 caatcatcca ttgggaggac ggaacagtca cagctgtccc agaagaagag ctaccattac
     7021 ggttgcctaa gacaaaaaac atcaagccaa gcggtactgg agaatctcca ttagcaaata
     7081 tcgaggagtg ggtcaatgtc gttgatcctg taacaggtaa aaaaggccgt cgcgaaacga
     7141 atacaatgcc acaatgggca ggaagctcat ggtattacct gcgttatatc gaccctcata
     7201 acaaaaaaga actagcagat tatgaaaagt tgaagcgttg gctaccagtt gacatctaca
     7261 ttggtggagc agaacatgcg gtacttcatt tactatatgc acgattctgg cataaattct
     7321 tatacgatat cggtgttgtt cctacaaaag aaccattcca aaaactatac aatcaaggaa
     7381 tgatcttagg cgaaaacaac gaaaaaatgt caaaatctcg tggtaatgta gtcaatcctg
     7441 atgatgtggt cgaaacttac ggtgcagata cactgcgcat gtacgaaatg ttcatggggc
     7501 cattagatgc ttcgattgct tggagcgaaa atggcttaga aggaagccgt aaattcttgg
     7561 atcgtgtatg gcgtttgatc gttgatgaga acaataaaat gcgtgaccgt atcactacat
     7621 tgaatgatgg caagcttgat aaagtgtatc atcaaacagt gaaaaaagtg acagaagact
     7681 atgaaaatct tcatttcaat acagcaatct cccaattgat ggtctttatc aatgaagcct
     7741 ataaagtgga cgctctgcct tatgaatata tcgaaggatt tgttcaacta ctcgcaccga
     7801 ttgctcctca catgggtgaa gaactatggg caatccttgg aaatgatggt ggtatttctt
     7861 acgcaccatg gccaacatac gatgaagcag cacttgttga agacgaagta gaagttgttt
     7921 tccaagtaaa cggtaaagtt cgtgcgaaaa gcaatgttcc ccgagactta ggaaaagatg
     7981 agctagaaaa agcagcatta gccaatgaaa tagtgcaaga atatattgaa ggcaaaacag
     8041 tcagaaaagt tattgcggta ccaaacaagt tagtcaatat cgttgctaat taattggctc
     8101 tttgtcaata aggactgatg agttgtgcaa aatcaaatct gggccagaat gaacttcatt
     8161 ctgtctcaga ttttttgtta tttcaaattg attaattgat tgatcaaaaa ggttttgatt
     8221 ttcatgttct caaacacgtt caatgtcttt ttcttctgaa tggtcttgta agagatctct
     8281 gtaatcatca aatttttgtc tattaatctt agtatttttt gatgtataaa ctacctgaaa
     8341 ttgagagtac ataccaaatt cataaatttt ttattagtgg gactagtgat tgtatggatt
     8401 ttgacgcata atgaatgaag tatgagaaaa ggagagacaa atggatcaag aactatttat
     8461 tatcgaagta ttatcagcta aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag
     8521 aataatgttc aagcttttaa gcttagtatt tttctctggt ttgctttttt tcggagctgt
     8581 atcgttccta tatcgccata tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt
     8641 aaataatttg tatcactttt cgtggggagt tggcggaata acaattgttg tgcttattgt
     8701 ttttcttcta cttatttggc tccgttacca tcaattgtcg cgtcatttcg atcaatggct
     8761 tagtcaaaga gcacaacagt ttttacataa taaaccaact tctcaagatg atgactatct
     8821 ttattttgag aagacttcga cgagtcaagc ttttcggata caaaaaagtg attgccagtt
     8881 attgctgaga tcacttgatg atttacctat ctacttaggc gaaagcaaat caaaattcgg
     8941 aatgaacaca ttccatattt ttttactaga aaacgaaccc agatgggaaa aaagccagtc
     9001 ttcgcctggt ggatacctga caactaagat gagtacatta gtcactggtt ttttcctcat
     9061 tcttttagct ggaacaatct attatgggag atacactcag ccaaaaatgt cttctcatat
     9121 aaatgctgct tctcacatga caatgaactc aactaacgat acgcatatac aaaagacaaa
     9181 tgaagggttg ttagctgatc ctaagcaaaa ggtaaatttg acaacacaaa agataaatga
     9241 tctcaaactt gatacagaga cgcactctct ctatatgaca acagatagtg gtgctaattg
     9301 ggtatttgtg ccgattgatt cgcaatggtt gcgttttggc gaatacaccc tgacaacggg
     9361 gctgattcct gaaggttatt ggatggacaa cacatatgat atttccgccg atttctcatg
     9421 gtttatctat tcttctgggg atgatgtacg tctgctgact tcaagagata atggtaaaac
     9481 atggcaaacg tctattatca cccaatcagt tcagccgata cgttatcgaa aagcggtatt
     9541 ttatggcaac gatggtctat ttatctgtac atcagccagt gggatgtctg cagaaacttt
     9601 gtacatttac actactatgg atggcggaat cacttggata aaaagtggaa gtacaatgat
     9661 caatcagcca gtacaaaatg tcagcttcgt tacaacttct ttaggttttg tttcgacacg
     9721 tgaaagtttg ttttatacaa acaacggagg acgctcattc aaagaatcag ttctgtcgat
     9781 tccggaaaac tatcagacag gggggataga tctttttcaa tcaccaaatg aagttactca
     9841 aataagcgcg aatacgttag agacaaaatt ttatttgttg aaaaaaggac caatcgatgc
     9901 agggaaaatg tttgcctgcc tcttccgttc aactgataac ggggaaacat ggcaattcgt
     9961 tcagcaacta tcgcaagtag aacaaggaga ttaaagaaga gagattgtga gcctgacgtt
    10021 cagcaccgag tcacaagaaa caaattcaaa aaacagcttc tcatatttgt tgaatagtgt
    10081 aactcgtgat tcggagttga caagagcaac aagaagcgat tttgaagtat agcttctcgt
    10141 attttacaag atcgtgtctt attgccgata ggttgtctct tgaacaccgt ttattcgtaa
    10201 aaagggattg tgacaagtct tttgtcacta cttaccgaac gaaactgaag gagctatcca
    10261 aaaagtatca ttagtattat tctttgtgtt tctagtggca ccactagtac atttaaaaaa
    10321 gctatcgatc atggactaat catacatgat cgatagcttt ttagataatg tttgttacgc
    10381 tatctgaaat tgactattat aaagactggc ataaagcgtt ggtgattgga gtagttcaga
    10441 atgtgttcct ttttcgacga tatcgccatt cttcattaca aggatcatgt ctgcattttc
    10501 aatcgttgac aaacggtgtg caatcacaaa acttgtccta tcttttgtca cagcttccat
    10561 tgcttgctga atcaatgctt ctgttcgtgt atctacgcta gaagttgctt catctaaaat
    10621 aacgacagga ggattcgcta gaatgactcg agcaattgtc aataattgtt gctgtccttg
    10681 agataacgtg ccgttttcgc tagaaatgat cgtatcatat ccgtctggca gtgttcgaat
    10741 aaagtgatca cattgtgcga tttcagccgc ttcgataatt tgttctcttg tggcatccat
    10801 tttcccgtag gcgatattat cagctaccgt tccttcaaac agccaggtat tttgtaaaac
    10861 catcccaaaa gaacgacgta gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa
    10921 ttcgatcttt cctttattca gttcataaaa acgcatcagc agattgacta aagtcgtttt
    10981 tcctgcacca gttggaccaa cgatcgctac cgtttgtttt ggctttactg aaaaatcgac
    11041 ttgattcatc aatattttat ctgatgtgta accaaactgt acatttttga aagctatttt
    11101 tcctttcggt tgttctaaaa gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc
    11161 taatatctca aatatcctat cgattgctgc cattgcggat tggatcgagt tgatcacata
    11221 agaagcggta gaaattggtt cagaaatctg attgatgtac tgaagataag cttgtaagaa
    11281 gccgatcgtc acaccacctg atagaacaag catagcaccg aataccgcac ttatgatgaa
    11341 ggcgagttga ttaatgaaac gaatggccgg atagatagca cagttcataa attgagcacg
    11401 tttaaaagcg ttgtattgct ttttatttac ttcgtgtata gcagcttctg catgagcttg
    11461 ctgattgaat gttttgacca ctagattacc agctaagatt tcttccattc gattatttaa
    11521 aacacccagt tcatactgat tctgttcaga aatctgtttg tttttgttcg caatcttagt
    11581 tgtaacaatt gaactgccaa cgatcagcaa taagacgagc aaagtaagct tgctttcgat
    11641 ataaaacagc atcagaccag cagcagctat tgttacgaca gaagtaaaaa attgattgat
    11701 acctgtcaat aacacttgag aaagttgatt gagaccggta gttgtccgac taatgatgtc
    11761 acctacttga tgattgtcaa aaaacgacat cggtaatgtt ttgaattttt ttgtgatttc
    11821 ttttcgcacc ctcaatgtga tcttttcgct taacgctgcc attgtccgtt cctgaataaa
    11881 tgaagtgatg ctgcttaaga ctgaacagcc gatcagcaat aagacaggga acaacaatgc
    11941 ttgtttgata tcactgaaag aaagctgtcc aatacctgtt tttcttattt ctgataagag
    12001 atcatcgatc ccgattccca tgatcaaggg catggctacg actaataggt tcccgaataa
    12061 actgcataga aacaatccgt aaaaatattt atgttcaggt ttgatcattt tccaaaaacg
    12121 tatcaaagca cgcgtgttca acgttttttt cttttccatc gtatcccttc tttctatatt
    12181 ccttgtgatt ttgcaaactc ctgataagtt tggcaattag acagtagttc ttcatgagta
    12241 ccttgtccaa cgatttttcc ttcatctaaa acaatgatgt tatctgcttg ttggattgta
    12301 ctgaggcgtt gagcaacaat cacgagagtt ttctccgaca tgtgtttatg aagggctgct
    12361 cttaatgctg catctgtttt atagtctaaa gcggagaaac tatcatcgaa tacatagatc
    12421 tctgctggtt tgatcaacgc tcgagcaata catatccgct gtttctggcc ccctgaataa
    12481 ttagaaccgc cttgagcaac aaagctgtcc aatccgtcag gaagggtgtc cactagttct
    12541 tctaattgag cgatcttgat cgctttagac aactcttctg ttgaggcata ttcatttccc
    12601 atctgcaagt tgcttaaaat tgtcccgctg aataagaaag ccttctgagg gacataactg
    12661 atgtgggagc ggatctgttc ttgagtcatt tgagtaagag gaataccacc aagggttact
    12721 tttcctatcg tagggtcact aagtctcaag agaagtttcg ccaaactact ttttccagat
    12781 ccggtcccgc caacgatcgc tgttgttttt cctttaggta ttgtgaaatc aatatcttct
    12841 agaactggat cagagatttc agtatattta aatgtgacat gttcaaaagc agctgcattt
    12901 gtctgatcaa aggttggttc agctactgtt ttgatcgtct cttcctcaat ttcgatctct
    12961 gcttgcagta tttcttctat ccgtcgtaat gaggcaaacg atttagggat aatgaccaat
    13021 accatagctg cgatcatcaa gtaagcaagt gtcagcatag agtattcgac tacagcagtc
    13081 acgacaccga tttgtaatgc tccttctgct acaagatttc cgccaaacca caaaatagaa
    13141 gcataaacga ttcccatgag caaaaaagca attggagtga cccaagcaaa tattcggttc
    13201 actttgatca tttgattagc atagtcagca aagctcttat tcgttcgctt ttcttcatat
    13261 gattgattat caaaggcacg gatcatatta atcccagtaa agaattctct caatgtgatg
    13321 gtgatccgat ccatttttgg ctggatactc aaagataaag gtgcccctcg tttcatcagt
    13381 cgataaacaa tccatacata aaaaacaaca gagatcaatg taacccatgt tagtaataga
    13441 gaataacgaa acatcatgag aatggtgaaa acgcaaataa ccggtgctgg taagatcatt
    13501 tggcaaaata aaacaatcat ttgctggaca ttatcgacat cattcgtcat tcttgtcaaa
    13561 agagatccag aactaaaacg actgatatcc ttcattgaca gctgttggaa tttacggaaa
    13621 tatttctccc gcgtttggaa tccaaaccta gcggaagcca atgctgaaaa gtaactagag
    13681 gcaactgcag cgattgttcc tagaaacgct acaagcagca tttcaactcc aatttttttt
    13741 ataactgagg cttcgccact tgctatacct gtgtcgatca gctttgctac taattttggt
    13801 acacctaatg taccggcgat ttctaaacag atcatcacaa aggtaaggag agcgagtcct
    13861 ttgtttttct gtaagaatgt ttttaagata gacatattat tcctcctttt ttcctgatac
    13921 ttttcgattc tataccttca agtaacttga aggtcaatag aaaaaagaat aaactgaaaa
    13981 agaggtgaga aaaaatgaaa aaaatgtata cgattggcca agtatcggaa ctatttgatt
    14041 tgccaaaatc aacgatccgc tattgggatg aacaaggatt aatccgttca agtagacaag
    14101 aagagaatga ttatcgattg tttgatatcg atgatatttt tatgctttat gatatcgttt
    14161 tttatagaaa attagatata ccgatcaaac agatgaagaa tttatatgga aaaacattga
    14221 cggagttata tgagacactt gatgaaacag aaagaagaat ccataaagaa ctggtggtga
    14281 tgaaacaaaa acaaaaagag ataagggagc aaaaaaaaca gttgaaattg atgatcgata
    14341 caaacgagga agaatttcct gtagaagaga tcccttttga ctgtatgatc tcaacggagt
    14401 ttgaagatat tgtggaaatc aaaaaatttc tacctaacta ttcaagcttt gggatgatga
    14461 gtatgccagc ttccagttca cagacgatgt atggattttt tatcgaccct agtgaagtac
    14521 atttatttac tcaagacgtg atttgggaga aaaaagatac ggctgtttat cgtcgattct
    14581 tattgaaatc cgagatgaac catgcggaac gtaataatat tttagaaatc agagaaagaa
    14641 tgtatgaaaa aggatggaaa acaggagaag tgatcggcca gtatctattg acgaatacgg
    14701 atgaaaacaa tatcagaact gaatattatc atgcatggat agaaatgaaa aaataggaaa
    14761 tatgtgttta agataactcg aaaatgatgg ttgttttagt tataaagaaa aattttctgg
    14821 ttcgattacg ggtttaatgt gggggattat tagtcgtgga tagatagaat tttagttttt
    14881 tagaggaaat atgtctttaa aaatggaatg gttgcaattc ttagcgatat atacgatgta
    14941 attgtcctct taaaaaagta tggaaaaata attgtattgg gtgttatgta taattcatgg
    15001 tgaactgaga ctaatgagaa gaaaaattca tattgttatg agtgagattg tatcagatga
    15061 gaatgataaa ttaaataggt gcataaaagc attacactta aaatacattg ttgcttacat
    15121 tattacaata tgttaataag gcaaaaataa atagatggat tgaataaaca tattttttaa
    15181 tcaaatagtc cctactgtga ctttcctcag ctatcggtga tactaccgat agcatttttt
    15241 tgcttataaa ataattataa gtatttagag atttttcttc taacattctt caaacaagaa
    15301 aaatcacaaa ggttattgtt atttttatat attcttttta agaatgattg tttttttata
    15361 gaaattttaa aaaataaaac acgttattga tggatagtta gtttgttatt tggtaatatt
    15421 atagaagttg actggtagtg atttattttt cgtcgtttta taaaaatgtt attttttgga
    15481 aaaattcaca aatatgtaac gaatgttttt ctggaatttg ctgtgttaaa aagcagtatg
    15541 actaatataa atactattgt catttatgca attttttatg gatagtaatt ttagagggga
    15601 atcaatgaag gaagaataga agggaaaaat agttgcaaat acgccagtta gcaaaaatag
    15661 caatacatct tcaagcttta aaattcgaga gaatcatctt ggtagtggaa tcaactacaa
    15721 caaaaggatt aaaccaatta atagatagaa ttaccaatgg aaaaatagaa aaggtagtta
    15781 ttctatacaa aagttgttta attcgcttcg gttatgaact tagtgaaacc ctttgcaata
    15841 tatggtacag aatatagaaa ttattgacat tacagaaaat gatgaagatt taatacagat
    15901 tgtcactatt tgtagatacc ttttacaagg aaaccgagcc aataaagcta agaagatgat
    15961 taaggagttg atggaagacg atacaagtta agaaagtgaa actacgccca aataaagagc
    16021 aaaaagagta attggacgtg gaaaatgcaa gaaatgaacc atcgattcgg tggacagttt
    16081 attagtaatg acggtttgag aacatccatg acgagaatga aggaacatcc taaatgcgtt
    16141 tagttgaacc aagtgccaaa taatatggcc aaataagcga tcagttatcg atgaatccca
    16201 agtattctaa actatatatt cattttgatg ctaagcattg atatattttt ttgggattga
    16261 tgaaggttaa ccaatacagg gactcactga tgaaattcta gagtctgacc ttggtatcaa
    16321 cgatttagtg attgtttcta atggaaaagt ataaaagtat ataagtacat taacaaaata
    16381 actattaggg ttacgctcca gcttgtgcaa ggcactggca ggaggaatcg tgaaatcata
    16441 attatggtta agccacacgg acaactaaaa gtcatacaaa gctatcgaat caatcaacag
    16501 aagacaatcg gcaatcatta gctttgttta cagcttacat tgcaacgctt gagttgaacc
    16561 gaaaatacga gggaggtaac gcagccattt tgagttgggg aatcttttta gaaaattatc
    16621 gggtaccttg ttgtttagtc aaaattataa gggggagtaa tagtgaggaa caaatttcat
    16681 aaactgttaa gtatgtttct agttatgctt attgtgatca atcaatttgg gaacataacg
    16741 gttctagctg agtctttagc agaacagcca gagcgttcgc tgacgatcga agttgatcaa
    16801 gaggaggctg ctttgggtga gcagttccag ttgcgagtca gaggaacacc agacctgcta
    16861 aatgcattgg tcattgataa gagtgaggat ttgattgaag gggaagtgaa gcaactcaat
    16921 gattacgaac gtctgattac ttattcttcc cagcattcag gtaaatacga attgaccgca
    16981 agaagcggtg atattcaatc aaatacggtc tcaatagaaa tcagtgtacc agaagaaagc
    17041 acaagtgagt cttttaatgt accagaagaa agcacaagtg aatcttttaa tataccagaa
    17101 gaaagcttaa gtgaatcttt taatgaagcc agatcatctg agccatctat aacgcaggaa
    17161 tctcagattg aaagacaaga ggagctcttg aattatgaca tcggcatctc tgctgagcgc
    17221 cccactttgc cgagcaggca tacacaaacg ttaaagggat acattcaagg aggcgctggc
    17281 gatattcttg aagaatatgg ggagatggtc gtacgagttc ctagagcatc gattggctta
    17341 attgagttca ttagagtgga ggtcgctgat gattcacctt taaagccact ggcattttac
    17401 caagaaggca cagacgttgt ttttccattt acctttgatt atgaaggatt aaatgaagcc
    17461 gctcagtcgt atacattcag tatcacgttt gaagttgctt tctttcaatc aggcggtact
    17521 gagccaaata atgactttca agtgagtctt acttatgctg agcgagcagc aaacacggaa
    17581 ttttcaatta cgcctattcc ttcatcaaga cccatttttt cgaaattata ctatggctct
    17641 agagaaatcg acagccaagg aaatcttggg aatctgctga ttgacgggca agacgtgacc
    17701 aacagtaata aatttatttt gcaggtaaat tatgaaggat cacgaaaaga cggtgtagtg
    17761 gttaccgatg tactaccgca aggaacaact ttggcaccga atccgactac atggagttcc
    17821 actggaaata gagaacctat ttcaggtggg gttcggatcg cagaagtaac gaatccaggt
    17881 gaagcaaacg ttagaactca ttatgtttcc gaggaatttg tggacaaagt atcttttaat
    17941 aaggaaacga atacacttgt tgtggacttt ggtaatctag cagagggtca atcgtatttt
    18001 attgaatatg ctcttgaagt gaccgatgtc gacttgttca atgaaggaag acaagaggat
    18061 gggttttctc ggaatcgagc gagtatgacc tacaataatc aggtaatgag ccgagactac
    18121 cgcttgagaa tccctgaaag taatcttccg gcaatctctt taagaaaaag tgtggacaaa
    18181 aatgttatca atctcaatga aaatgaacta acgtatactc tgcatgtctc gatgttttct
    18241 ggaacaagaa atacgcttgt tattgaagat gttttggaag aaggcatgga atttgtgtca
    18301 tttctatcag atattccctc tttcattgat gccgaagtca ataatggaat agttcgtttc
    18361 agtgcgaata atacgattgg tggtccgaca agttttaccc tctcttttaa ggtcaatgta
    18421 gaggcgtatg cggtcggcga tcaaatttta aactttgcaa cgcttgtgaa tagtgacaat
    18481 cgattgaata cgagtactgt aagaacaagg aaaatcgatg ggcgtattaa agtaattaaa
    18541 actgatgaga atggccaaag gctggcagga gcaaaatttg aaattttatc aagcgattca
    18601 caagtgatac aagaaggtat aacggcagaa aacggagaat ttttaagtga accgcttcct
    18661 gtaggtagct atcaagtccg agaaacagaa gcaccggaag ggtatctttt agatgcgacg
    18721 gtacatgatg tttttataaa tgaatactcc atagaagaag tgacgttaac cattgaaaac
    18781 atccttgata cgggtggtgt tgaactaatt aaacatgctg aaggcagtgg tgaagtcctt
    18841 caaggagctg tttttgagtt acagaaccga gaaggagaaa cgctacaaac cggtttaact
    18901 acaggagaag atggaaaact ggcaattgac ggtttagcac caggagcata tcagctggta
    18961 gaaac
//
LOCUS       DASHFF010000050        17834 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.050, whole genome shotgun sequence.
ACCESSION   DASHFF010000050 DASHFF010000000
VERSION     DASHFF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.050"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            123..935
                     /locus_tag="U2I75_001970"
     CDS             123..935
                     /locus_tag="U2I75_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN1697076.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            932..1732
                     /locus_tag="U2I75_001971"
     CDS             932..1732
                     /locus_tag="U2I75_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN1697077.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1893..3161
                     /gene="rseP"
                     /locus_tag="U2I75_001972"
     CDS             1893..3161
                     /gene="rseP"
                     /locus_tag="U2I75_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN1697078.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3229..4938
                     /locus_tag="U2I75_001973"
     CDS             3229..4938
                     /locus_tag="U2I75_001973"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN1697079.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5150..9502
                     /locus_tag="U2I75_001974"
     CDS             5150..9502
                     /locus_tag="U2I75_001974"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN1697080.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9646..10119
                     /gene="rimP"
                     /locus_tag="U2I75_001975"
     CDS             9646..10119
                     /gene="rimP"
                     /locus_tag="U2I75_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN1697081.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10143..11318
                     /gene="nusA"
                     /locus_tag="U2I75_001976"
     CDS             10143..11318
                     /gene="nusA"
                     /locus_tag="U2I75_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN1697082.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11340..11633
                     /locus_tag="U2I75_001977"
     CDS             11340..11633
                     /locus_tag="U2I75_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN1697083.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11630..11941
                     /locus_tag="U2I75_001978"
     CDS             11630..11941
                     /locus_tag="U2I75_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN1697084.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11954..14260
                     /gene="infB"
                     /locus_tag="U2I75_001979"
     CDS             11954..14260
                     /gene="infB"
                     /locus_tag="U2I75_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN1697085.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14287..14634
                     /gene="rbfA"
                     /locus_tag="U2I75_001980"
     CDS             14287..14634
                     /gene="rbfA"
                     /locus_tag="U2I75_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN1697086.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14742..14963)
                     /locus_tag="U2I75_001981"
     CDS             complement(14742..14963)
                     /locus_tag="U2I75_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1697087.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15411..17774
                     /locus_tag="U2I75_001982"
     CDS             15411..17774
                     /locus_tag="U2I75_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEN1697088.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6095 a         3154 c         3920 g         4665 t
ORIGIN      
        1 ttaaaaaaag aaaaaattat tcagttttta aataaaaaaa caatgatttc ataggaaaaa
       61 cagttagata ttgctataat acttaagata gtgggaatct tttaagtata ggaggaaaaa
      121 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
      181 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      241 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      301 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      361 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      421 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      481 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      541 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      601 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      661 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      721 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      781 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      841 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      901 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      961 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
     1021 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
     1081 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1141 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1201 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1261 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1321 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1381 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1441 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1501 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1561 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1621 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1681 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1741 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1801 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1861 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1921 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1981 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     2041 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     2101 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2161 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2221 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2281 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2341 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2401 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2461 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2521 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2581 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2641 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2701 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2761 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2821 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2881 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2941 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     3001 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     3061 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3121 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3181 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3241 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3301 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3361 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3421 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3481 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3541 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3601 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3661 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3721 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3781 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3841 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3901 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3961 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     4021 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     4081 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4141 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4201 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4261 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4321 ttccactata aaaatgtgaa tccaaaacgc gatttcactg tgctttctta tgaagatctt
     4381 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4441 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4501 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4561 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4621 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4681 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4741 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4801 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4861 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4921 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4981 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     5041 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     5101 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5161 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5221 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5281 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5341 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5401 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5461 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5521 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5581 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5641 agtggatgag cagcaagcgg aacgtgtatt gaaacttttt gaagaacgca aacaagaaca
     5701 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5761 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cagctaggac gaaatattcc
     5821 agccgatgaa ccaacgacac caatgatcag tatcgttgaa gaagaacgaa gagtcacact
     5881 tgaggggtac gtattcgaca aagaagtcag agaactacgt tcaaaacgca agatcttgac
     5941 tttgaaaatc acagattata cttcatcatt tatcgtcaaa aaattctcga acggcgaaaa
     6001 agatgaacaa gtatttgatg cgatcagtgt cggtagctgg ttaaaggtac gaggaagtat
     6061 tcaagaagat acgtttgtcc gtgatttggt catgaatgcg caagatatca tagaagtcaa
     6121 acatacacct cgcaaagatt atgctcctga aggggaaaaa cgagtagagc ttcatgttca
     6181 tagcaatatg agtacgatgg atgcaacaaa cagcatctct gatcttgtgg cacaagcagg
     6241 aaaatgggga caccgagcta tcgccattac agaccatggt ggtgcgcaag cctttccgga
     6301 agcacatagt gctggaaaaa aagcaggtgt gaaaatctta tacggcgtgg aagcgaatgt
     6361 cgtagatgat ggtgtcccaa ttgcttataa tgatgcacat gaagcattga gtgaagccac
     6421 atacgtcgta tttgacgtgg aaacgacagg attgtctgca gtatatgaca cgatcatcga
     6481 gctagccgca gtcaaaatgt ataaaggaaa tgtgatcgaa agctttgatg aatttatcga
     6541 tccaggacat ccattgtcac gaacaacggt agatctgact ggtatcacgg atgaaatggt
     6601 tcgagggtca aagtcggaag aagaagtatt gcggatgttc cttgaatttt caaaagatac
     6661 aattttggta gcccacaacg cggcttttga tatggggttc ttgaatacta gctacgcaag
     6721 gtatggtatt cctgaagcgg ccaatccagt cattgatacg ttggaattag ctagatatct
     6781 gtatcctcaa ttcaaacgtt tcggattagg tgtgttatct aaaaagtttg gtgttagctt
     6841 ggaacagcat caccgagcaa tctatgatgc tgaagcaact ggtcacttag catggatctt
     6901 tgtgaaagag gcaatggaca atcataacat gctttatcat gatcagctaa atgagcatat
     6961 cggagaagga gattcttaca aaagggctcg acctttccat gtaacgattt tagcaaaaaa
     7021 tcaggcagga ctgaaagatt tgttcaaact gatttcaatg tcgaatgtcg aatacttcga
     7081 acgcgtccca cgaataccac gctcccaatt gaaaaagatg cgtgaaaatc tattgatcgg
     7141 ctcagcttgt gataagggag aaatattcga agccatgatg caaaaagggg tagaagaggc
     7201 aagaaaccga gcaaaattct atgattatat tgaagttatg ccaaaagcgg tctatgcgcc
     7261 tttgatcgag caagaattgg taaaaaatga acatgatttg gaagaaatta tccaaaattt
     7321 ggtagaaatc ggaaaaagtt tggataaaat cgttgttgca acaggtaatg tacattatct
     7381 gaacgaagaa gatgccattt accgaaaaat tttgatcaat tctatgggcg gagcgaatcc
     7441 gttgaatcgt catagtttgc cggatgtcca tttccgaaca accgatgaga tgctaactgc
     7501 atttcacttt ttaggggaag aaacagcgaa agagatcgta gtagaaaata cgaataaaat
     7561 cgcagatatc tgcgaagaag tgattccagt caaagatgaa ctttatacac cgaagattcc
     7621 aggttcagaa gatgaaatat cagaattgag ttacacaaaa gcaaaacaaa tgtacggaga
     7681 tccgttacct gagatcattc aaaaacgatt agaaaaagag ttgaattcta ttaatggtaa
     7741 tggcttctcg gttatttatc tgatcgccca aaaactagtg cataaaagta atgaagatgg
     7801 ctatctagta ggttcacgtg gctctgttgg ctctagcttt gttgcgacga tgacaggaat
     7861 cactgaagtt aatccattag cccctcatta ttattgtcca gagtgccaat attccgagtt
     7921 ttttgaagat ggtacgtatg gttctggatt tgacatgcca gaaaaacaat gtccaaaatg
     7981 tggtgcacgc ttaaacaaag acggtcatga tattccattt gagacatttt taggattcca
     8041 tggagacaaa gtgcccgata ttgatttgaa cttctcaggt gattatcaag cagaagccca
     8101 taactatacg aaagtattgt ttggcgaaga ctatgtttac cgtgccggaa cgattggtac
     8161 agtcgccgac aaaacagcat atggctatgt aaaaggatac gaaagggaca ataatctgca
     8221 gtttcgaagt gcagaagtcg acagattagc aaaaggcgct actggagtca aacggacaac
     8281 cggccagcat ccagggggga tcatcgttat tcccgattat atggatgtct atgattttac
     8341 gccgatccaa tatccagcag acgatcaaaa ttcagaatgg aaaacgactc attttgattt
     8401 ccactcgatt cacgataatg tattgaaatt agatattcta gggcatgatg acccaactgt
     8461 tattcggatg cttcaagatt tatcagggat cgacccacaa acgatcccta cggatgaccc
     8521 ggaagtgatg cggatatttg ccggtcctga agtacttgga gtaagtcagg aacaaattta
     8581 ttccaaaaca gggacattag gtattccaga attcggcact cgttttgttc gagggatgtt
     8641 agaagaaacg catcctacaa cttttgcaga actactgcaa atttccggac tatctcatgg
     8701 tacagacgta tggttaggaa atgcggaaga attgattcga cgaggagatg cgacactggc
     8761 ggaagtaatc ggctgtcgtg atgatattat ggtttatttg atccatgctg gattagatag
     8821 cgggatggca tttaaaatca tggaaactgt tcgtaaagga caatggaaca agatccctga
     8881 tgaattgcga gaaacctatc tatcggcgat gaaagaaaat aatgtgcccg actggtatat
     8941 cgattcttgt tcaaaaatca aatatatgtt tccgaaagcc catgctgcag cttacgtatt
     9001 gatggctctt cgtgtcgctt atttcaaggt ttatttccct attttgtatt actgtgcata
     9061 cttttctgta cgtgcagacg actttgatct agtctccatg tgcaaaggaa aagatgctgt
     9121 gaaacaagca atgaaagaaa tcacggacaa gggattagat gcttccgtaa aagaaaagaa
     9181 tcaactgaca gtgctggagt tagccaatga aatgctggaa cgaggattca aatttggaat
     9241 gattgatttg tataaatcgg atgccgttaa ctttgttatt gaaggtgata cgttgatcgc
     9301 accatttaga gctgtcccaa gtttgggaac caacgtggcg aaacaaatcg ttgaagcaag
     9361 aaaagacggt ccatttttat ccaaagaaga tcttgccacc cgcggtaaag tttcaaaaac
     9421 attgatcgaa tatatgaacg acaatggcgt attgaaagat ctaccagatg aaaatcaatt
     9481 atcgttattc gatatgctat aagcatctgt tttcctgtct gcttgtaatt ttaagcagag
     9541 tatggtatag ttcttatagg taatgatact aactgtgagt gagcggattt ttcgctcact
     9601 ctttttaatg gaattacgca cttaattttt aaggaggcga aacttttgag tagcgtcgtt
     9661 gaaacagtca cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa
     9721 gtcgaatttg taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc
     9781 ggtatcgata tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca
     9841 gcagatccag atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag
     9901 cggccattga aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc
     9961 ttataccaac cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct
    10021 gaacagctta cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga
    10081 aaaaatattg caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa
    10141 aaatgagtaa agaaatgtta aatgcgttag acgctttaga agctgaaaaa gggatctcaa
    10201 aagaaatcgt gatcgatgct ttagaagcag cattggtttc tgcatacaaa cgtcactatg
    10261 gccaagcaca aaacgtcgaa gtggaatttg atcagaaaaa aggaaagatc catgtctatg
    10321 cggtaaaaga agtaacggaa gaagtgatgg attcacagtt ggaagtctca ttgaaagatg
    10381 cacttttaat caatccagca tatgagatcg gtgatacgat ccgttttgaa gtaacaccaa
    10441 aagattttgg acggatcgct gcgcaaacgg ctaaacaagt tattttacag cgtgtacgtg
    10501 aagcagaaag aacgattatt tataacgaat tcagtgctta tgaaaaagac atcatgcaag
    10561 gaatcgtcga acgccaggat aaacgttata tttatgtcaa tcttggtaaa attgaggcag
    10621 ttctttctaa acaagatcaa atgccaaatg aattttatca accacatgat cgaatcaaag
    10681 tttatgtttc ccgagtagaa aatacgtcta aaggtccgca agtatttgtt agccgcagcc
    10741 atccagacct attgcgtcgc ttatttgaac aagaagtacc agaagtctat gatggaatcg
    10801 ttgaaattgt cagcatcgcc cgtgaagctg gcgatcgttc aaaagtggca gttcgctcaa
    10861 atgatccaaa tatcgatgca gtcggtacat gtgtcgggcc aaagggacaa cgagttcaag
    10921 caatcgtcaa tgaattaaaa ggcgaaaaca tggatatcgt cgaatggaat gaagatccag
    10981 ctgtattcat cgcaaatgca ttgaatcctg cacaagtcat ggatgtgatc ttcgatgaga
    11041 caaatccaaa ggcctgcaca gttgtcgttc cagactacca attatcgcta gcaatcggta
    11101 aacgcggaca aaatgcacgt ttagctgcta aattgactaa ccataaaatc gatatcaaat
    11161 cagaatctga catgacggaa ttttatgaaa atcaagaaca acaaaaagaa acagaagaac
    11221 tgcatgatga agcaatcgtt cagtcagatt taacagacga tgaatatgaa acaattgcat
    11281 tcaacaatga aacggtcgaa gaaaaaccag aagcttaact ttagtaagga ggcaaagaga
    11341 tgaaacaaag gaaaattcct ttacgcaaat ccgtggtttc aggtgaaatg aaacctaaaa
    11401 aagagatggt ccgaatcacc cgttcaaaag aaggggaagt atcgatcgat ccaactggaa
    11461 aaatgcctgg acgaggagcg tatgtctcac ttgagccgga agaagtacaa caagcttggg
    11521 ataaaaaaat tctcgatcga gtactcgaaa caacattaac agatgagttt tatcaagaac
    11581 ttcttgacta tgtcactcac caaaaagcac gtaaagagtt atttggaaaa tgaatacagt
    11641 taatcggcaa aaagccatga atctcatcgg tctagcgatg cgcgcgggaa aactaatcac
    11701 gggtgaagaa ctgaccattg ctgaaatccg caaacaaaaa gcaaaattgg tgtttgtggc
    11761 aaccgatgca agtgagaata caagaaaaaa gataaaagat aagagttcat attatgaagt
    11821 tccttgcttt gagctatttt ctgaagcaga aatcacgcag atgatcggaa aacctcgcaa
    11881 ggtattcgga ataatggatg ccggatttgc gaagaagacc aaggagctaa ttgaaggtta
    11941 ggaaggtgat tgcatgggca acaaaagaat ctatgaatta gcaaaagaac tcaaaaaatc
    12001 tagtaaagac gtcgtggaaa aagcacaaca acttggcatc gatgtgaaaa accatatggg
    12061 tgcgattact agcagtgatg agaagaaatt gcaacaagcg ttcaaaaacc caagcacgaa
    12121 acaacaagct gcacaacaag caagcagaaa accagccaat caacagccga acgatcaacg
    12181 taaaaaagaa acaacgaaca atcaacaaaa aaataaaaat aaccgcagtt atcaagatcg
    12241 cggtcaaggg agcggtcaag tgaatcaagg aaaaaaacca acaacaaacc aaaataatac
    12301 gggaaatacc agcagccaaa atcgtcaagg aacgagccaa ggcggcaatc aaaaccgtac
    12361 taacaataac aacaaaccaa acaataacaa tcgcggcaaa tttaataata ataaccgtaa
    12421 tcgttataat aaaaaaggta aaaaaggcaa acagcaacaa tcaactaaac cagctgtacc
    12481 accacgcaaa ttccgtgaat tgccagatgt attagagtat actgaaggta tgaacgtagc
    12541 agatattgcg aaaaaaattc atcgtgagcc agcagaaatc attaaaaaat tatttatgct
    12601 tggtgtcatg gtcaatcaaa accaagcact ggataaagat acgattgaac tgttagcgac
    12661 ggactatggc atggaaccac aagaaaaagt ccaagttgac attgctgata tcgataaatt
    12721 cttcgaggca gaagaagtcg atccagataa attagtctca cgtccgccag tagtaacgat
    12781 tatgggacac gtagaccacg ggaaaacaac cttattggat acattgcgcc attcacgtgt
    12841 aacaagcggc gaagccggcg gaatcacaca gcatatcggt gcctaccaga tcgatattga
    12901 tggtaaacca atcactttct tggatacacc aggacatgcg gcctttacaa gtatgcgtgc
    12961 acgtggagca agtatcacag atatcacgat cttagttgtt gctgcagatg atggcgtcat
    13021 gccacaaaca gttgaagcaa tcaaccatgc caaagctgca ggcgttccaa tcatcgtcgc
    13081 tgtaaataaa attgataaac caggtgcaaa tcctcagcat gttatgcaag aattaagtga
    13141 gtatgaatta attcctgaag catggggtgg agaaacgatt tttgttgaga tctcagcgaa
    13201 attcggccaa aacatcgaag aattgctgga aatgatcctg ttagtcgcag aagtagaaga
    13261 tttaaaagca gatccaacac aacgcgcaat cggaacagtg atcgaagcac gtcttgataa
    13321 aggtaaaggt cctgtgtcaa ccttacttgt ccaagaagga acacttcacg ttggtgaccc
    13381 aatcgttgtc ggtaatactt acggacgtgt ccgtgtaatg acaaacgatc ttggccgtcg
    13441 cgataaagaa gcaggacctg caacgccagt agaaatcact ggcttgaatg atgtacctca
    13501 agcaggtgac cgtttcgtag tcttcgaaga cgaaaagaca gcacgtgcag cgggtgaaga
    13561 acgcgcaaaa cgtgcattat tggaaaaccg tgctgccaca agccgtgtga ctttggacaa
    13621 cttatttgaa agcctgaaag aaggcgaatt gaaagaagtt aacgtaatca tcaaagcaga
    13681 tgtacaaggt tcagcagaag cactggctgc atcattgaag aagattgatg tggaaggtgt
    13741 ccgtgtaaaa atcgtccatt cagctgtcgg agcaatcaat gaaagtgatg taaccttagc
    13801 agcagccagc aacgctatta tcatcggttt caacgttcgt cctacaccgc aggcaaaagt
    13861 gcaagctgat actgaagaag tcgatattcg tcttcaccgt atcatctata aagccatcga
    13921 agaaatcgaa actgcgatga aagggatgct agaccctgaa tttgaagaaa aaatcaccgg
    13981 tcaaatgatc gttcgtgaaa ccttcaaagt ttctaaagtt ggtacgattg caggtgccta
    14041 tgtaacagaa ggatatattc gtagagatag cggtgtccgt gtaatccgtg acggaatcgt
    14101 aatctatgaa ggccaacttg caagtctgaa acgattcaaa gatgacgtga aagaagtcaa
    14161 aatgggttat gaatgtggtg cgatgattga aaaattcaat gacatcaaag tagatgacgt
    14221 aattgaagga tttatcatgg aagaagtcaa aaatgactaa aaactgataa aaggaggcca
    14281 atcattatgg ctaactaccg tgatcgccga gtcggtcaag aaatcttaaa agaagtcaat
    14341 gatattttgc gcaaaaaggt ccgtgaccct cgtgtggaga acgtaacgat cacagatgtg
    14401 aaagttacgg gggacttaca gcaagcaacg atctattaca gcattttatc tgacttagca
    14461 tcagataaga aaaaagcaca agatggattg aataaagcat caggattgat tcgtcgagag
    14521 ttagggcaca atctaagtat ttataagaca cctgaattgt tctttgaact tgatgaatca
    14581 gttgtctatg ggaaccatat cgatgaattg cttcgcgact tgaacaaaga ctaatcaaaa
    14641 aatcccgcaa atcctgtgaa atcaggggtt gcgggatttt tttgttttca ttaagacgta
    14701 ttacctaaga gttccatgac tttagtctgg tagcgtgtca gctaaccaaa aaagagaaaa
    14761 agaaaagtaa aaaattcgac tttctttttt ctatagtata taaatttttg catatagata
    14821 aagatcaaga taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat
    14881 agccaagtaa ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg
    14941 ttgacagtta aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga
    15001 tttggtcacg atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac
    15061 caattcacat attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag
    15121 gaattgctcg atccagcccc agttttccaa caatgtgtta actggcctag cgtcattcag
    15181 caaattatgc atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag
    15241 ttttttggat caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct
    15301 gttcatttgg ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat
    15361 tgggagtatc tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt
    15421 ctagtaaaga atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg
    15481 ttgggcaact ttatcttaaa gataacccct tactgagacg tacactgaaa ccagaagatg
    15541 tcaaaaaaca tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc
    15601 atttgaaccg agtcatcaat aaatatgatt tgaacatgtt ttatatcgaa gggccaggac
    15661 acggcggaca agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc
    15721 cagaagtaac ggaagatgag acaggaatgc aaaaattatt caagcgattt tcatttcctg
    15781 gaggcattgc ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc
    15841 ttggatactc tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg
    15901 ctgtcgtgat tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa
    15961 atgtctttat caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg
    16021 caaaaatagc caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact
    16081 ttaacggact tggctgggag cctttcttta ttgaaggaaa tgatccagaa aaattaaatc
    16141 ctgttatggc tgaaaaaatg gatcaagcaa tcgagaaaat caaatccatc caaaaagaag
    16201 ctcgcctgaa gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca
    16261 caccaaaagg atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc
    16321 gagcacacca agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac
    16381 tccgttggct gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag
    16441 aagaaatcag agaaattgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca
    16501 atggtggaat cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat
    16561 ttgaaaaacc aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg
    16621 agatcattga gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa
    16681 accgattgaa tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag
    16741 aaaatgatga atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc
    16801 aagatgaagg gttcttagaa gggtacgttc tgactggacg gcatggtttt tttgcaagtt
    16861 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatacgga
    16921 aatcacatga cctttcttgg cgaaaagatt atccttcgct gaatctcatt gcaagctcaa
    16981 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    17041 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    17101 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17161 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17221 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17281 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17341 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17401 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17461 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17521 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17581 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17641 tcaaaggaag tcaagcagaa gattttgcgg aaaaaatgga tcagaaatta caagaacatc
    17701 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17761 atcttgacca ataaaatgat aaaatcgatc ccgcaaaaca gaatttttta cattgtatta
    17821 tttgtcaaat taag
//
LOCUS       DASHFF010000051        17168 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.051, whole genome shotgun sequence.
ACCESSION   DASHFF010000051 DASHFF010000000
VERSION     DASHFF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.051"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(40..849)
                     /locus_tag="U2I75_001983"
     CDS             complement(40..849)
                     /locus_tag="U2I75_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1697089.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1142..2578
                     /locus_tag="U2I75_001984"
     CDS             1142..2578
                     /locus_tag="U2I75_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1697090.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2748..3944
                     /locus_tag="U2I75_001985"
     CDS             2748..3944
                     /locus_tag="U2I75_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1697091.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4006..4191)
                     /locus_tag="U2I75_001986"
     CDS             complement(4006..4191)
                     /locus_tag="U2I75_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN1697092.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4422..7736
                     /gene="dnaE"
                     /locus_tag="U2I75_001987"
     CDS             4422..7736
                     /gene="dnaE"
                     /locus_tag="U2I75_001987"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN1697093.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            7904..8866
                     /gene="pfkA"
                     /locus_tag="U2I75_001988"
     CDS             7904..8866
                     /gene="pfkA"
                     /locus_tag="U2I75_001988"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN1697094.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8940..10724
                     /gene="pyk"
                     /locus_tag="U2I75_001989"
     CDS             8940..10724
                     /gene="pyk"
                     /locus_tag="U2I75_001989"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN1697095.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10759..11661)
                     /locus_tag="U2I75_001990"
     CDS             complement(10759..11661)
                     /locus_tag="U2I75_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1697096.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11847..12416
                     /locus_tag="U2I75_001991"
     CDS             11847..12416
                     /locus_tag="U2I75_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN1697097.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12474..12653
                     /gene="rpmF"
                     /locus_tag="U2I75_001992"
     CDS             12474..12653
                     /gene="rpmF"
                     /locus_tag="U2I75_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1697098.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12823..14244
                     /gene="gndA"
                     /locus_tag="U2I75_001993"
     CDS             12823..14244
                     /gene="gndA"
                     /locus_tag="U2I75_001993"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN1697099.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14437..15123
                     /locus_tag="U2I75_001994"
     CDS             14437..15123
                     /locus_tag="U2I75_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1697100.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15120..16625
                     /locus_tag="U2I75_001995"
     CDS             15120..16625
                     /locus_tag="U2I75_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1697101.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5554 a         2980 c         3594 g         5040 t
ORIGIN      
        1 aattgtcaca acctcttttt tgtggaatct aattctctat tatagtttaa gtagtttttc
       61 cagataaaca gctaaaccgt cttcctgatt gctcaaagga gtgacatcat ttgcaatgcc
      121 tttgagttgg tcagtaccgt tagccatagc tactccccaa cctgcataat ccaacaattc
      181 caaatcatta tgctcgtcac caaaggcaat gacgtctttt tgatcgatag acaggtagtt
      241 agcaatctct tgcacgcctt ttgctttttg gattcctttt gaaacaatct ctaagatggc
      301 agtaggtcca ccccatgtcc gaacatcgac ataactgcca aattgacgtg ttaattcagc
      361 agatacagct cctgcaaaac gtttgtctgt tcggacaagc aaagatgtag gatttgtcgt
      421 caaattttta ggagaaagca aattcttctc tcctggacga gaagaagcaa aaattttttc
      481 atcaaagaaa tcaaaactgt caataaaaaa agtatcacga ttttcagcag cgataaaatc
      541 taaatttagc tgcttttttt gcgataagat ttcaaaagcg atttcacgat tgatcaacgt
      601 ttctctttca ccatcccagt gctgattagg gatgtgtact aatgcgccgt taaaattaac
      661 catcggtgtt gttaattcta attgttggta aaactgatgg ctcattcgga atgggcgtcc
      721 tgtagcaata ctgacatgat gaccggcatt gattgctttt tttagagtct cttttgtttt
      781 agatgtaatc atcgaatctt gattgagggt agttccgtcc agatcgatgg caattaattt
      841 tttattcaat aagggtcctt ccttcctcaa ctagcatttg ttttagtcta gcatatttta
      901 ggtaattaac aagagcaatc agctattcct aacaaaaact acagttatgg ctatataaat
      961 agctaaacat aagctaaaca cgaacgttaa gtattatttt cgcttttttt gttaaaaaaa
     1021 cgttgaaaag cgaatgaaac gtctatataa tttgaaattg ttcgatggat ttgccctatt
     1081 ttacttattt tttattatta tagttttcca tcagaaaaaa attcagggag tggaactttt
     1141 tatggaaaag tttttcggtt taaagaaaaa caatacaaat gtatcaactg aaataatggc
     1201 gggagtaacg acattctttg ccatgagtta tatcttattc gtaaatccga cgattttgtc
     1261 agcatcaggt atgcccttcc aagcagtatt tctagcgaca atcattgcat cgattattgg
     1321 tacgctggta atgggattgt tcgcaaatgt accatatgct caggcacctg gtatgggatt
     1381 gaacgcattt ttcactttta cggtcgtttt tgggcttggt tattcttggc aacaagcctt
     1441 ggcaatggtg tttatttgcg gattaatcaa tatttttatt actgtcacaa atattcgaaa
     1501 aatgattatt cgggcgattc ccgaaagtct tcaacatgcg attggtggcg ggatcggtat
     1561 tttcgttgcg tatgtcggaa tcaaaaatgc aggattctta tctttttcag cagaccagtc
     1621 agctatttcg agctctgtcg ttgaaggcgg aaaagcgaca aatgttacga taaatggtgg
     1681 aatcgtccct gcgttagcca actttgacaa cgctcctatc ttattggctg tgattggttt
     1741 ggtactgaca gccattttag ttgtaaaaaa tgtccgtggg gctattctta tcggtatcgt
     1801 agcgaccaca gtccttggta tttttatggg tgttgttgat ttaagttcca ttgattggca
     1861 aacgaattcg ttaggtaatt catttaaaga attaggaaca actttcggtg cagcatttgg
     1921 ttctgaaggc atgcaatctt tattcagtga ttcttccaag attcctcaag tattgatgac
     1981 gatcatcgca ttcagtttat ctgatacttt cgatacgatc ggaacattta tcggtacagg
     2041 acgccgcaca ggtattttct caaaagaaga cgagatcgcg ttggaagatg gtcgtgggtt
     2101 caaaacaaaa atggataaag cactatttgc cgatgcgatc gctacttctg tgggggctat
     2161 ctttggtaca tcgaatacaa caacttatgt cgaatctgca gctgggatcg gggccggtgg
     2221 acgtactggt ttgacttcag tagtagtggc agtgctattc gcattaagta gtttattctc
     2281 tccactgatc gccattgtac caaatcaagc aactgcgcca gcattgattt tagttggagt
     2341 aatgatgatg gcttcttttg cagacatcaa atggctagat atggaagaag ctttaccggc
     2401 cttctttgca tcgatcttta tgggattgtg ctatagcatt tcctatggga ttgcagcagg
     2461 atttattttc tatacaattg tgaaagtaat caaaggaaaa tacaatcaag tttctgttgc
     2521 tttgtggatc gttgatattt tattcattat caacttcatc attctagcaa caatttaact
     2581 aaagagaagg gattggaaca gtatttgttc tgatctcttt ttgttttaca taaaaagaac
     2641 gttacatata aaatgacctt tgcgggattt ttctcttttt actggcgaga tcgttgcttg
     2701 tttattcgaa aataggtatg ataaaagaga cgaaagaggg atgatagatg gagattaatt
     2761 ggagaaaaaa tttagtgatt gcgtggattg gctgtttttt cactggtgca agtattagtt
     2821 tagttatgcc attcatacca gtttatgttg agcaactagg aacaccaaaa gatcaaatcg
     2881 aattgttttc gggactagca atctctgtga ctgcttttgc atctgccgtg gttgcaccaa
     2941 tctggggaaa tctagctgat cgaaaaggcc gaaaactcat gatgatccgt gcagcagcag
     3001 ggatgacatt aacaatggga tctttggctt ttgtaccaaa cgcgtattgg cttttgatca
     3061 tgcggttttg gaatgggatt ttgtctggat atattccaaa tgccacagcg atgattgctt
     3121 cacaggcacc tagagaaaag agcggatggg cgttaggaac attgtctacc ggagcgattg
     3181 ctggaaatct gatcggacct tcaatgggcg gtgcactggc tcagtggttt ggtatggaaa
     3241 atgtattctt gatcacaggg gctttgttga tgatcacaac ggtattgacc attttcttag
     3301 tcaaagaaga tttccatcca atcgagaaaa aagatctgat aagcaccaaa gaaatttttt
     3361 caaaaatgga tcatctctct attttgatcg gtctcttcat tacgaccttg attttacaga
     3421 taggaatcac tacgatcagc cctattttga ccttatatat tcgtgaattg agcggaagta
     3481 cagaaaatat cctttttgtc agcggcttga ttgtttcgat agctggtgtt tccgctgtct
     3541 tttcttcacc gaaactagga aaactagggg ataagattgg gaatcaaaaa gttctaattg
     3601 gaggactcat tttatcattt tgctgttatt tacctatggc attcgtcact acaccgctcc
     3661 aattaggtat acttcgcttt attttaggct tttcaactgg cgctttgatg ccttctgtca
     3721 atacactgat cagtaagatt acgcctcaag aaggagtcag ccgaatctat agctacaatc
     3781 agatgtttag caactttgga caagttttag gtcccatgct aggatcgaca gtcgcgcatg
     3841 cttataatta cagtgctgtt tttattgtga caagtttgtt tgttctgtca aatattcttc
     3901 tttctttatt caactttcgt aaagttcttc gcagcagatt ataatggtgg tgaggagtag
     3961 aaaataaaaa agagcttgag aaatctcaag ctcttggctg tttttttacg aatgggcaga
     4021 ggtttctttt cttggcgcaa ctgcaccaat caaaaggata atgacacccg caatgaatga
     4081 aatgattgtt gtcaaagtaa aatcatatgt tccgcttgtt aatgcagcac caatataacc
     4141 gactacttga cctagaacga cagcccagaa taatgtaacg atatatcgca taaagaacac
     4201 ctcttttcta tcttaatttt accagatttt ctttgaatgt aaagaaacga gaccaaatct
     4261 caataaaatt tgagttcttg cgaaagaact cattcctaca gattacaagc aagcaaatga
     4321 agtggaataa tcaacttcag cgcctgctat cttaatgaaa gtaaaaggat caaacctttt
     4381 aacgctagtg aattttaacg aaaaaaaagg cggaatgcaa gatgtttttc ccacaacttt
     4441 atacaattac ttcttattct cttttacaaa gtacgatacg tattcgagaa tatgtacaag
     4501 aagcaaaaaa acaaggctac acttcattag ccataacgga taaagatgtc ttatacggtg
     4561 cacctgaatt ctatcgtatt tgtgtagaag aaggagtaca gccaatcatt ggattacatc
     4621 ttagttatac ttatgaagaa aaaaattatg gcttgcttgc gtacgcaaaa gatgaaatag
     4681 gttatcagca gctgatgaaa ctttccagcc gaaaaatgat ccaagaaaaa ttggaacttg
     4741 cagaaatgac agaaaccgac catctagcgt ttgttgttcc ggcaaatcat tctgtttctg
     4801 aggctttcaa taaagaggat aactttttcg gtcattggca gtttttactt gaaaatgttt
     4861 ttactagaaa tttttatatc ggcttgacag aaaacacgcc tgatcaattg cctgaatggt
     4921 atcagtatgt gataaaagaa aattattcgc tttgtgcatt acatcccatc gctagtttac
     4981 aacaagaaga actttttgca cttgaagtga tgaaacatat caaagaggga agccaaatac
     5041 ctttagaaaa tttatctgca tctttaccac taacagcaaa tgtctatctt ctttcagaac
     5101 aagaaatgat caaacgattc gaaaaagcgg gtctttccca agctgtaata ggcgcccagc
     5161 gtttagctga atcgatttca tttgaattcc agttccatca gaaattactt ccgcattatc
     5221 cagttcctaa tggaaaacaa gctggagaat atttaagaga gttatgctat tcgcaagtgg
     5281 aacgccgaat cactgactat acaaaggaat accaagaacg tttggactac gagttatctg
     5341 tcatccatca aatgggattc gatgattatt tcttgatcgt atgggatgta atggcttttg
     5401 ctcatagaaa caaaatcgtt accggtgcag gtcgtggttc tgctgcgggt tcattagtag
     5461 cctatgtgtt agaaatcaca gatgtggatc cattagaata tgatttgctg ttcgaacgct
     5521 ttttaaaccc cgaacgcttt acgatgccag atatcgattt ggatattcct gacaatcgac
     5581 gtgaagaagt attgaattat gttcgagaaa agtatggaca atatcatatg gcacaaatcg
     5641 caacatttgg cacaatggca gcaaaaatgg ttcttagaga tgcagctaga gtgtttggtt
     5701 tatcgcaaag tgaagcaaac cgctggtcaa aagcaatacc caatcaattg aaaatcacat
     5761 tgacccaagc ttataaaact tcgcatcaat tagtagaatt agttcaggca agcgaaaaaa
     5821 accaactttt attcaaaact gcagtgattt tagaaggatt gccaagacat gtttcgactc
     5881 acgcagcagg tgtagttatc agcgataaga atctacttga actagttccg ctccaacgcg
     5941 gttctgatga agcttttctg acccagtata cgatgaatga ggtagaagca atcggtcttt
     6001 tgaaaatgga ctttttagga ctacgcaact tatcgattat tgattttact ttgaaagctg
     6061 ttaagcgagt agaagggtat gagatccgat taaaagagat cccgctaaat gatgcaaaaa
     6121 cgctgctctt gtttcaacga ggcgaaacct caggagtatt tcaatttgaa tctgctggta
     6181 tccggaacgt tttacgaaga ttagatccag aaaacatcga agatattgcc gcagtcaatg
     6241 cattgtatcg accaggccct atgcaaaata tagatacctt cattgccagg aagaaaggaa
     6301 aagaagccat tcgctaccca gatgattcac tgatcccgat tttgaaaaac acatatggag
     6361 tgatcgtcta tcaggaacaa atcatgcaga tcgcctcgac tatggctgga tttacattag
     6421 ggcagtcaga tattctgcga cgggcagtga gtaaaaagaa aaaagacgta ttagatgaag
     6481 aaaagtcgca ttttgtatca ggagcattac aacagggata ttcggaaaaa acagcagcga
     6541 ccgtatatga atatatcgaa aaatttgcaa actatggatt caatcggtca catgcatttg
     6601 cttattcatt cgtgggattc caaatggcgt atctaaaagc ccatcatcca ggtgctttct
     6661 ttaccagcct gatgaattct gttcggcata acacatcaaa attgaaagaa tatattgcag
     6721 aagcacgtaa aaataaactc aaactttcct cgccgtccat taatcaaagt aattccagtt
     6781 ttgagttggt caataaggaa gaaatcagat tcggactgac agcgataaaa ggtattcggc
     6841 gtgattttgt agaagatata ttaaacgaaa gaaaacaaaa tggcccgttc cagtcagtgg
     6901 atcaattcct catacggatc gataagcgtt ggctgaaatt agagttactg caatcactga
     6961 cagcagtagg agtttttgat gaattatctc ctaacaggaa acaattgatg cttgatctcg
     7021 aaggaaaaat ccaaaatatc gtttacagtg gtggaagtct tgacttgctg ggaatcatgg
     7081 cattaaaaga agaagaagtc gctgactata cactagaaga acgcttgaag ctggaagagg
     7141 agtacttagg tatttatttg tcaggtcatc ctgcagaagg gtttgatcgt cttcgtttag
     7201 ctaaacaagt tcagctgatt acagatatcg tccctaatca accagtcaat ttattagtga
     7261 tgatcaagga agttagagaa attcggacaa aaaaaggaga actgatggcc tttgttgaag
     7321 gaaccgacag tagtggagaa atttctgtca cagtttttcc aactctttat cgtaaatatc
     7381 gtaatttgtt tgccgttgat cgagtgatct ttgttcaagg gcggagcgaa atcagcaaat
     7441 ataatcagga actgcaacta attggagaaa gctttgctga tcctaaccag ttagaagccc
     7501 agtatccttc acaaacttgc tatttgcgta ttacagaaga acgggaagat cccgagataa
     7561 tgaaacaatt acaagtgatt tttcagaaac acccaggata tataccagta gttatgtatc
     7621 acgaaaaaac gcaacgaaaa gtagttctgt cagatgctta ttgggtggat gacagcaaga
     7681 gtataaagcg gcaacttatt tatctgcttg gcgaagataa tgtggttttc aaataactta
     7741 cggtaaaaaa tactacttgc tttcattttt tttcattttt ttatccgtaa atcaagacaa
     7801 gtgagaaaaa taatgttaga atgttagtcg tggttaacgc aaaaatggtt gtaaacctaa
     7861 gtcatactta ggataaagta ttttaatgag gtgaatgttt attatgaaac gcatcggaat
     7921 tttgaccagt ggcggagacg cacctggaat gaatgcagct gttcgtgccg ttgttcgtaa
     7981 aggtatttac gaaggactgg aagtatacgg gatcaattat ggttttgccg gtttagttgc
     8041 tggagatatt cgtcgtcttg atgtggcaga tgtaggtgac aagatccaac gtggtggtac
     8101 attcttgtat tctgcacgtt atccagaatt tgcaactgaa gaaggtcaat taaaaggaat
     8161 cgaacaattg aaaaaattcg gaatcgaagg tctagtggtt atcggaggag acggctctta
     8221 tcatggcgca atggctttga caaaacgcgg tttcccagca gtaggtattc ctggaacaat
     8281 cgataatgat attcctggga ctgatttcac aatcggtttt gatacagcaa tcaatacagt
     8341 actagaatca attgaccgta ttcgtgacac agcgacttct catgttcgta cattcgttat
     8401 cgaagtaatg ggacgcaatg caggagacat cgctttatgg tcaggtgtgg ctggcggagc
     8461 ggatgaaatc atcatcccag aacatgattt tgatatggct tcagttgcta aaaaaatcca
     8521 agaaggccgc gatcgcggga agaaacattg cttgatcatc ttggctgaag gtgtaatggg
     8581 cggaaatgaa ttcgctgaga aactttcaga atttggtgac tatcatacac gtgtatctat
     8641 cttgggacac gttgtccgtg gtggagcacc aagcgcacgc gaccgcgtgt tggcaagtaa
     8701 attcggtgct tacgctgtcg aattgcttca agaaggaaaa ggtggattat gtgtcggtat
     8761 ccatgacaac gaaatcgttg cgtctgacat cattgataca cttgaaaaca acaaacataa
     8821 accagactta tcactatatg atctaaataa ttcactttca ttttaagagt gaaagaaacc
     8881 aagctattta agctgtgtac agctttaaat aaacaaaatt gaatgggaga gttttttaaa
     8941 tgaaaaaaac caaaatcgtt agtacattag gaccagcaag taactcagta gaaattattt
     9001 ctcaattgat tgaatctggg gcaaatgtct tccgctttaa tttttctcat ggtgaccatg
     9061 aagaacaatt atcacgtatg actatggtcc gtgaagcagt taaaaagaca ggaaaagatg
     9121 tcggtattct tttagataca aaaggagctg aaatccgtac aactgttcaa ggcacaacag
     9181 aagctgattt tggtcgtgca ggatacatca aatttgaagt aggagatcaa acacgcatct
     9241 ctatggatcc tgaacatgta ggttcaaaag aaaaaatcgc ggttacttac ccaggattat
     9301 ttgacgatgt tcacgttggc ggacacattt tatttgatga tggtttgatc gatatgcaaa
     9361 tcattgaaaa agacgaagca aaccgtgaat tagtcgtaga agttaaaaat gcaggtatgc
     9421 taggatcacg taaaggggta aatgcaccag gcgtttctat tagtttgcca ggtattactc
     9481 caaaagatgc tgacgatatt cgttttggtt tagacaacga tatcgacttt atcgctgcaa
     9541 gttttgtacg taaagcacaa gacgttctag atatccgcga aatcttagaa gaaaaagaca
     9601 tgactcatgt tcaaatcttc cctaaaatcg aatctcaaga aggtatcgac aacattgacg
     9661 aaatcatcaa agtttctgac ggtgttatga ttgcccgcgg agatatgggt gttgaaatcc
     9721 cagctgaact agtaccaatg gttcaaaaac gcattatcaa aaaatgtaat gctgctggta
     9781 tcccagttat cactgctaca caaatgttag aatcaatgca agaaaaccca cgtccaacac
     9841 gtgcggaagc ttctgacgtt gctaacgctg tatttgatgg cacagatgca acaatgctat
     9901 ctggtgaatc tgcaaacggg gactacccag ttgaagctgt ttcaacaatg gcacgtatcg
     9961 accaagaagc tgaaaatgca ttatcagaat taggtacatt ccaattgaac gaattcgata
    10021 aaacagatgt aactgaaaca atcggtttat ctgtagctcg tgctgcaaaa aatctaggcg
    10081 tgaaaacaat cgttgctgca actgaatcag gatacactgc aaaaatgatt tcaaaatatc
    10141 gtccagatgc agatatcttg gctgttactt ttgacgaacg cacaaaacgt ggtttgatgt
    10201 taaactgggg cgtttaccca acagttgctg aaaaaccatc aacaactgat gaaatgtttg
    10261 aactagcagc taaaaaagct gttgaattag gtttcgcatc agaaggcgat ttaatcttga
    10321 tcactgctgg tgtaccagtt ggtgaacgcg gaacaactaa cgtgatgaaa attcaaatga
    10381 ttggttctaa attattagaa gctcaaggcg ttggacaaaa atctgtcgtt gcaaatgcag
    10441 ttattgcttc atctgctgaa gaagctgtaa aaaaagcaaa agacggaatg gttctagttg
    10501 taccatcaac tgacaaagaa tatatgcctg caatcgaaaa agcagctgct gttatcgttg
    10561 aagaaggcgg attgacttct cacgctgcag ttgtaggaat tgcacaagat attcctgtaa
    10621 tcgttggtgc taaaggcgca acaacaacaa ttacagaagg tgaattgatt accgttgatt
    10681 cacgtcgcgg catcgtttac cgtggcgaaa caacagctat ctaatttagc ttaataaaaa
    10741 ataaaaacag ctcttctttt acgaagagct gtttttttgt gctgtaacga attcatggat
    10801 aacttggact acttgttctt gaagaggcgt gacgatctgc tgtgttcgtt ttgccaatga
    10861 aatcaagaag cgaggctgaa cttcatctga aagagggagg caaactaaat gatcttcagg
    10921 atggacagca atctctgcca aaaaaccgat acccacattt tctcggacca tactctttaa
    10981 aatcgtcaag tcattgcttt gataaacaac ttttggatga gtatgtgttt gtttagaaag
    11041 cttagtgaat gctgcggggt ggacataatg ttcgttcaat agtacaaact gttcatctgc
    11101 tatatctttg aaagagacag tatcctgctt ggaaagagga tgtgtaggtg gcacgacgat
    11161 catgaaacgt ttgtccaaaa gtaactctgt ttctaaatat tgatccacga ttggctgtgt
    11221 tgagcctaat acagctaaat cgatcctccc ttgtcggatc atcccgtata agtcccgaga
    11281 gcctccatcg gtaatctcaa tttgattcat caattcatgc ttgataaagt aacttgacag
    11341 tttaggaaag tattcattgc cgataatagg aggcatgcca aaacgaagct ttttcttttg
    11401 caactgagtg atttcagctc ttgcagtaga aatctcttgg agaatggttt gtgcatggag
    11461 aaacagctgc tttccagaag gcgtgacgat taattcgtga tgcgatcgat ctcttgtaat
    11521 aagtacggtg tcaaactctt tttcaagacg ttgtaatgca tatgtaatag taggttggct
    11581 cacacggaaa taattagcca ctttcgtaaa atttttttgt ttgacgattt ggacatagta
    11641 ttcgatatct ttcgtgttca tgaagatcat ccgctccttt aatggaataa tgattattgt
    11701 aaatgattct gaggatgata gcaaacagag gctttctaat aaagattttt ctgttttttt
    11761 atgaactgca aggtgtcaag aaaatttcat tgacaacatg ctttttgccc tgtataattg
    11821 cttttgttgc ttaggagtga tgaaaaatga aatggtcttt attggaattg agaaaatacc
    11881 aggagacacc gttaactttt catgagacgc tcgacttgaa agccgctctc atgaagcgag
    11941 acaatttaat acttgatgtt gcgccagtgg aagtcgaagg tttggtctca gttgataaga
    12001 aaggctatat cgttcattac actgtgcaga cgactttgac agttccttca acacgttcac
    12061 tagaacctgt tgaactgccc atggattttt ctgtagacga agtattcatg acagaagaac
    12121 aattccaaag tagagatgaa caaatgtctg cggatgagat tttgctaatt gaaggtccat
    12181 tattgaattt agcagagtcg attgaagaca acatcttatt ggcgattcca atgcgggtat
    12241 tgacaaaaga agaggaagca gcaacagaac ttccaaaagg caatgattgg gaagttgttt
    12301 ccgaagaaga ataccagcgc agaaaaatgg aagaagccga gaataaaatt gatcctcgtc
    12361 ttgcaaaact atctgaactt tttgattcta aatcaaaaga cgacgataac aagtaagatt
    12421 ggaatatatt gtgtctaatt tagatacaag actttacaag gaggtgtagc aaaatggcag
    12481 taccagctag aagaacatca aaagctaaaa aagcaaaacg tcgtacacac tacaaattga
    12541 caatcaaagg gttaaacgaa tgtccaaact gtggtgaaat gaaaaaaagc catcacgtat
    12601 gtgcaaattg tggttactat gatggtaagg acgtaatgtc aaaagaagct taatacattt
    12661 taattagaaa atgtaaaacc tcaaggctaa ccatggagcc ttgaggttta tttttttgta
    12721 gtcggtaaat aaaaaatatg ttgttcttta gtttgcaatg tagccgtgtt aagctataat
    12781 gtaagagatt agggagaaat cccgtatgta ggaggagttc atatgtcaaa acaacaaatc
    12841 ggcgttgtcg gtatggccgt tatgggtaaa aatctagcct tgaacataga aagccgcggc
    12901 tattcagtgg ctttattcaa tcgtacaggg gctaaaacaa ccgctgtagt agaagaacac
    12961 ccagataaaa atttcaaagc aacatataca atcgaagaat ttgtagaatc tatcgagaaa
    13021 ccacgtcgga tcttgctaat ggtcaaagca ggaccggcaa cagatgcaac gatccaagag
    13081 ttgcttcctc accttgataa aggcgatatt ttgattgacg gaggaaatac attctttaaa
    13141 gatacgatgc gtcgtaatga agagttagcc aactctggca tcaactttat cggtacaggc
    13201 gtttccggtg gtgaagaagg agctttaaaa ggaccttcaa tcatgcctgg tggacaaaaa
    13261 gaagcctatg cgctagttgc accaatctta gagcaaattt ctgcaaaagc agaagacgaa
    13321 gcaccatgtg tcacatatat tggcccaaat ggagccggac attatgtgaa aatggtacac
    13381 aacggtattg agtatggaga tatgcaactg attgctgaat cttatgactt aatgaagaat
    13441 attttaaacc tttcagtaga agaaatggct gatatcttta aagaatggaa tcaaggcgaa
    13501 ttagacagct atttgataga aatcactgct gacattctga cacgtaaaga cgatgaagga
    13561 acaggtaaac cagtagtaga tgtgatcctt gatgcagctg ggaataaagg aacaggtaaa
    13621 tggacaagcc aaagcgcgct tgacttaggt gtgccacttc cattgatcac tgaatctgta
    13681 tttgcccgct atatttctgc ttataaagaa gaacgcgtac aagcaagcaa gattttatct
    13741 cgtacgaacg attttgagtt cacgggagac aaaaaagaat tggtcgaaaa aatccgagaa
    13801 gcgttgtatt tcagtaaaat catgagttat gcccaaggat ttgctcaatt acgtgtcgct
    13861 tcaaaagaat ttgattggga cttgccattt ggtgaaatcg caaaaatctg gcgcgctgga
    13921 tgtatcatcc gagctcgatt cttgcaaaaa attacggatg cctatgacaa aaatcctgag
    13981 atcgaaaatt tattgctaga tgattacttc gttgaaatca cgaaaaaata tcagcaatca
    14041 gtcagagacg tcgttgcctt ggctgtccaa gctggcgtac ctgtaccgac tttctcatca
    14101 gccatcgctt acttcgattc atacagagca gaacgtctgc ctgcaaatat aatccaagcc
    14161 caacgcgatt actttggcgc acatacctat gaacgtgtcg acaaagaagg aatcttccat
    14221 tactcatggt accatgaaga ataatagtct ttcggctgtt aggaaatgag aaaataaaaa
    14281 gaggacgaaa gagctgaagt cgattgttat gagtagactt cagctctttt ttaaaattgc
    14341 tatccatgat aaaatgacta tagattttta agcatattat cggtataatg atagagtttt
    14401 taaaaagcca gtagaataga aaggataaaa taataaatga gcaatattct aatcattgaa
    14461 gatgaaaaga acttggcacg atttgtcgag ctagagttga agcatgaagg gtataacgca
    14521 gaagtccact acaacggccg aactggttta gatgcggcat taaataatga gtgggatgcc
    14581 atattgttgg atctgatgct cccagaattg aacggtttag aagtctgcag acgtgtacgc
    14641 caagtcaaaa atacaccaat cattatgatg actgcaagag attctgtgat cgatcgtgta
    14701 tctggacttg accacggagc agatgactac atcgtcaaac catttgctat cgaagaatta
    14761 ttagcacgtc ttcgtgcatt gttgcgtcga atcgatattg aaggcgataa aaatgtggct
    14821 aaacaaacta ctattactta tcgcgatttg actatcgaaa aagaaaaccg tgtcgtacgt
    14881 cgcggaaatg aagtaattga actaacaaaa agagaatacg agttgctgct tactttaatg
    14941 gaaaacgtga acgtggtatt agcacgtgac gttttactaa ataaagtgtg gggatacgaa
    15001 acagaagttg aaacaaacgt agtggacgta tatattcgtt atcttcgcaa caaaattgac
    15061 gttcccgggg aggaaagcta tatccaaact gtacgcggaa caggttatgt gatgcgctcg
    15121 tgataaagcc gaaaaaatcg gaaaaaagag aactgaaagg gccctccttg acaatcaagt
    15181 gggcctttgc gagttctttc ttcatattcg ttgtgtttac aattttcgct gtcattacct
    15241 ataaatcatc tatcaatctg attgtggcaa aagaaagaaa caatgtagag cgtacgattt
    15301 cagaagtagc ttctcgtttg gctaactcgg atgaagaact gacattagta aatacgtatc
    15361 gtaatctgac gaaagcgaca aatgacggag atgaggtcgt agatcatact tcactggtcg
    15421 aaggaaactt aatgaagatc gattccttta tagctgagct agggcagcca tcaatggatc
    15481 tttatgttta tgatttggac gagaacttga tttttaaaac ccatgaaaac agtcgaaacc
    15541 tgattcagac tacacgtaaa gttccaacga tcgttacact agaaggaaaa actggctttt
    15601 tatctatcca gcctatatat tctaaaggta caagagaaaa gatcggctat gcgcaaagct
    15661 tctatgaatt atcttctttc tatgatattc gaaataagct attattgact cttattgttt
    15721 tagaagttgt ttctttgatc ttaagtagta ttttaggttt ctttttatct tcttatttct
    15781 taaaaccact caaggtactc agagatacaa tggatactat acgtaaagat ccgcaatcag
    15841 atatccacat gcctgaaatc gacacaaatg atgaattggc agatttagct gaaatattca
    15901 atgagatgct tgaccgtatg cgtttgtata ttgaacaaca agagcaattc gtggaagacg
    15961 tatctcatga gttaaggaca cctgttgcaa ttattgaagg gcatcttagt ctgctaaatc
    16021 gttgggggaa agatgatcct gagatattag aagaatcatt aagcgccagc atgcaagaaa
    16081 ttagccgtat gaaaagctta gttcaagaga tgctagactt atcaagagca gaacaagtag
    16141 atgttcatta tgcgaatgaa acaactaaag gaaaagaagt cacctatcaa gtttataata
    16201 atttcaagat attatatcca gactatgtga tcactttaga tgatgatttg acaaaagaag
    16261 tgaccctgca aatttatcgt aatcactttg aacaattaat cattatcata ttggataacg
    16321 cagtaaagta ttcaactaca cgaaaagaag tccatatctc tatttcttcc actctgaatg
    16381 aatttgagat cgcgattcag gactttggcg aaggaatacc caaagaagac cttaataaga
    16441 tcttcaatcg tttttatcga gtggataaag caagagctag aacgaagggt ggaaatggtc
    16501 tggggttgtc tatcgctaaa caactggttg aaaactataa aggacgtatc ttagcagaaa
    16561 gtgtagtagg gcaagggaca atttttagaa tctttattcc tatcgcaaag gcagaagaca
    16621 agtaaagcat gtgctttact tgttttttta tatatccaac aaagaataag gtacaaaata
    16681 caagcaagtt agaaattcag ggatgtcatt ataaaaaaaa ctagcgaaca attaaataga
    16741 ataacatccg ttaattaaac gtaagtagct tttagatgaa cgaaaaaaca atgaaatgct
    16801 gtaatattcg tgtttaaaaa taataaataa aaatagggaa ttttctattg caattttata
    16861 aagagattgc tatattatta tttgttctaa atgattcctt taaggtgtca ctaaaaatta
    16921 taaaaaatag ggttgacgca cttttgatgt catgttataa taacgaagtc gtttggatga
    16981 ggtagctaat aaagcttgat tttatcgaaa aaaacttcga aaaagttgtt gacaaataac
    17041 aataaagatg atatgataat caagtcgctg ctaatgagtg acaaagacgt ttagaaaaaa
    17101 taaacaaaga agttgttgac attctcttct gaatctgata agattaaaga gttgttgaaa
    17161 caaaaaga
//
LOCUS       DASHFF010000052        17007 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.052, whole genome shotgun sequence.
ACCESSION   DASHFF010000052 DASHFF010000000
VERSION     DASHFF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.052"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..126)
                     /locus_tag="U2I75_001996"
     CDS             complement(<1..126)
                     /locus_tag="U2I75_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEN1697102.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="U2I75_001997"
     CDS             complement(230..541)
                     /locus_tag="U2I75_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1697103.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="U2I75_001998"
     CDS             complement(543..740)
                     /locus_tag="U2I75_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN1697104.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="U2I75_001999"
     CDS             complement(1134..2861)
                     /locus_tag="U2I75_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN1697105.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="U2I75_002000"
     CDS             complement(2854..4047)
                     /locus_tag="U2I75_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1697106.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="U2I75_002001"
     CDS             complement(4234..4878)
                     /locus_tag="U2I75_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1697107.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="U2I75_002002"
     CDS             complement(4972..5106)
                     /locus_tag="U2I75_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN1697108.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="U2I75_002003"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="U2I75_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN1697109.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="U2I75_002004"
     CDS             complement(7237..7710)
                     /locus_tag="U2I75_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN1697110.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="U2I75_002005"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="U2I75_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN1697111.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="U2I75_002006"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="U2I75_002006"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN1697112.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="U2I75_002007"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="U2I75_002007"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN1697113.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="U2I75_002008"
     CDS             complement(11757..12896)
                     /locus_tag="U2I75_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1697114.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="U2I75_002009"
     CDS             complement(13205..13918)
                     /locus_tag="U2I75_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN1697115.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="U2I75_002010"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="U2I75_002010"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN1697116.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="U2I75_002011"
     CDS             15107..16639
                     /locus_tag="U2I75_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1697117.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="U2I75_002012"
     CDS             complement(16830..>17007)
                     /locus_tag="U2I75_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1697118.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DASHFF010000053        16605 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.053, whole genome shotgun sequence.
ACCESSION   DASHFF010000053 DASHFF010000000
VERSION     DASHFF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.053"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..515
                     /gene="scm"
                     /locus_tag="U2I75_002013"
     CDS             <1..515
                     /gene="scm"
                     /locus_tag="U2I75_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1697119.1"
                     /translation="SDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDT
                     TSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHA
                     SVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRI
                     TFMSYKKSKR"
     gene            complement(670..1656)
                     /locus_tag="U2I75_002014"
     CDS             complement(670..1656)
                     /locus_tag="U2I75_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1697120.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1679..2245)
                     /locus_tag="U2I75_002015"
     CDS             complement(1679..2245)
                     /locus_tag="U2I75_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN1697121.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2259..2675)
                     /locus_tag="U2I75_002016"
     CDS             complement(2259..2675)
                     /locus_tag="U2I75_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1697122.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3132..5066)
                     /locus_tag="U2I75_002017"
     CDS             complement(3132..5066)
                     /locus_tag="U2I75_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1697123.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5347..6303
                     /locus_tag="U2I75_002018"
     CDS             5347..6303
                     /locus_tag="U2I75_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN1697124.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6393..7496
                     /locus_tag="U2I75_002019"
     CDS             6393..7496
                     /locus_tag="U2I75_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN1697125.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7509..8462
                     /gene="menA"
                     /locus_tag="U2I75_002020"
     CDS             7509..8462
                     /gene="menA"
                     /locus_tag="U2I75_002020"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN1697126.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9397..10743)
                     /gene="gor"
                     /locus_tag="U2I75_002021"
     CDS             complement(9397..10743)
                     /gene="gor"
                     /locus_tag="U2I75_002021"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN1697127.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10877..12130)
                     /locus_tag="U2I75_002022"
     CDS             complement(10877..12130)
                     /locus_tag="U2I75_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1697128.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12136..13059)
                     /locus_tag="U2I75_002023"
     CDS             complement(12136..13059)
                     /locus_tag="U2I75_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1697129.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13280..15772)
                     /locus_tag="U2I75_002024"
     CDS             complement(13280..15772)
                     /locus_tag="U2I75_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN1697130.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15779..16246)
                     /locus_tag="U2I75_002025"
     CDS             complement(15779..16246)
                     /locus_tag="U2I75_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN1697131.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16382..16455)
                     /locus_tag="U2I75_002026"
     tRNA            complement(16382..16455)
                     /locus_tag="U2I75_002026"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16419..16421),aa:Arg,seq:acg)
     gene            complement(16513..16586)
                     /locus_tag="U2I75_002027"
     tRNA            complement(16513..16586)
                     /locus_tag="U2I75_002027"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16550..16552),aa:Pro,seq:tgg)
BASE COUNT         4904 a         3472 c         2957 g         5272 t
ORIGIN      
        1 caagtgacag tacagatact acctctacca caagtgatag cacggacacg tcagcttcaa
       61 gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca gcttcaagtg
      121 acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc actacaagtg
      181 atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc acaagtgata
      241 gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca agcgatacac
      301 gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac tttaacttaa
      361 ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct aaaactggaa
      421 gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc ggtttaagaa
      481 tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat aaaaaagagc
      541 aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac aagaactaca
      601 attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac gtatcgaacc
      661 tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga gattctcttt
      721 tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt ctgtgtaaga
      781 ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt tgcgtacggc
      841 gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta gctttggttt
      901 gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca tgtcttctag
      961 tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat cgataatctg
     1021 aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc gctcgcttgt
     1081 tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg tcttacctga
     1141 aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat ccatctgccc
     1201 taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac ttttcaagga
     1261 agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc ctgcatagac
     1321 agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca gatccgcttc
     1381 atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag aagctgccaa
     1441 agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata gctgataggc
     1501 aggacgcaac agctttccgc cggcatggat cgtttctttg atggcttgtt cgacaggttt
     1561 gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt ctttacttaa
     1621 agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg tgtgacgatt
     1681 attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa tctttttgga
     1741 atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg attgcaatcc
     1801 ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc catgattgtg
     1861 caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg gccgcactaa
     1921 ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt ccaaaataac
     1981 taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct agcaaagtag
     2041 tcaggaatag tcgtagccaa agtagtaaga agctgtcttg ttttttcatg gtaaaaatgg
     2101 caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa gaaaaaggat
     2161 aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt agcgaaatat
     2221 aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag actgtcttcg
     2281 tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca gacgatggtt
     2341 tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca atctgcttct
     2401 tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg ttcatctttg
     2461 taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat ttctgtgcca
     2521 tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag accaaaaatc
     2581 atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc ccaaggtttg
     2641 agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt tccagattaa
     2701 ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc tttgagtaat
     2761 aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct tattaccgag
     2821 aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg caacttcaag
     2881 aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc ggcttatttt
     2941 tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga ggttgtgaca
     3001 cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt ttacataaaa
     3061 aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt caaataacgt
     3121 tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca ccaattacct
     3181 agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg tccggaaccg
     3241 ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa catgcctgat
     3301 aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt gaataatcca
     3361 gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg gaagaacatt
     3421 gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc tggtgacttc
     3481 tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg tgcagcttga
     3541 gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc atcaccagca
     3601 gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg gaacacactg
     3661 tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac ttcaccattc
     3721 ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat acgaagtggc
     3781 acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt gcgatcatct
     3841 ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga acgaatatca
     3901 aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc gcttaaatgg
     3961 atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat aaatccttga
     4021 tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat attcgctgcg
     4081 gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt tcctgggaat
     4141 tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta gccttttgcc
     4201 tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc aaagtcagca
     4261 gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg aatgttgcgt
     4321 ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt caataataat
     4381 tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg atccaacata
     4441 ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg atctaattta
     4501 taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc aataccggta
     4561 aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc atctggttcc
     4621 aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga atcttcaaat
     4681 gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt cggctctccg
     4741 cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt gacgattttt
     4801 cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt ttgacgagca
     4861 aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc agctacttca
     4921 tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat tgctacatct
     4981 tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata tcctgctcct
     5041 acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta tctatataat
     5101 gaatatatga taattctttt agtttgatac ttattacaca atctccatat attatataga
     5161 tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa taaagcgttt
     5221 tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt tatcaaaaat
     5281 agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa aaattgtgga
     5341 ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac tggatttgcc
     5401 gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa cggaagctca
     5461 aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac aacagaatca
     5521 tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt agaagaaaaa
     5581 aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg caaaatcact
     5641 gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga tgcagaatac
     5701 aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc tgagttgaat
     5761 gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg agctactcac
     5821 tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca agctggtaac
     5881 acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta caagcttgaa
     5941 gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc tggcggcaaa
     6001 gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca agatgataca
     6061 gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta catccctact
     6121 ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc taatgtagaa
     6181 gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca acaattagtg
     6241 aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt aacagaaaaa
     6301 taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga ttaggcagtc
     6361 gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta ttttccttaa
     6421 tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca aataaaacaa
     6481 ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat gaacagttct
     6541 tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca gcattgaaac
     6601 ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa aaaggttctg
     6661 agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct gatgacgtct
     6721 acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga ttcgatatgg
     6781 cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga aaaccttccc
     6841 aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa ctaaatgaca
     6901 aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt gcaatcgcca
     6961 aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg accactgcca
     7021 tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc gaaaaccaag
     7081 actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta ggttctatta
     7141 aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta gaagtagatg
     7201 gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac aatgatatcg
     7261 caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct acagcagtct
     7321 tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat aacgggacgg
     7381 aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc aaagacacct
     7441 tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta aaataaggag
     7501 tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca aaggtggcta
     7561 gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat gagatacatt
     7621 ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg actaccgcta
     7681 tcaataatta catggattat gaaaaggctc attctgaagt atataagtat gaagaaaatg
     7741 tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta gctatgattg
     7801 catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt ttacttttga
     7861 tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt cctttatctc
     7921 gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt tttgccatgg
     7981 tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac ttttcatctg
     8041 gacaatttga tttggtagga aatctttggg ctatagcagc aatcgtatta gcttccttac
     8101 ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat cttgaccgtg
     8161 atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg ggaacagtct
     8221 tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg ttgagcggta
     8281 tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag aagcatttgg
     8341 atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc aaaaatatgg
     8401 tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg caagccattt
     8461 aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa cagccaaata
     8521 aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga
     8581 acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt
     8641 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa actggctttt
     8701 gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt
     8761 tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt
     8821 tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt agagaaacaa
     8881 atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag gacagctgat
     8941 tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc acagtccctt
     9001 ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg tttggtaact
     9061 atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac aaagtcacac
     9121 ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga taccaatatt
     9181 ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta cgaagataat
     9241 caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa tcttacataa
     9301 ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt tacatggttt
     9361 gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta cgaattcttc
     9421 cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg cacccatctt
     9481 gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag catgtaagcc
     9541 gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt ggcgataatc
     9601 atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt cattttcgcc
     9661 atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg gatgcgtaaa
     9721 gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat taaataaacg
     9781 ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt taccgatcac
     9841 atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta ctttgataaa
     9901 cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg tattcggtac
     9961 acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt caaatagcac
    10021 cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt aagtttttat
    10081 tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa acgttctaag
    10141 tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa atacacctga
    10201 aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta actctgttaa
    10261 ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca ttttacttgc
    10321 acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt ttacttcgat
    10381 cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat tgctatctaa
    10441 tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt ctactagacc
    10501 ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat ctcgttcaat
    10561 tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac cgacgttgac
    10621 acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat gcatccctgc
    10681 tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat catatatttg
    10741 cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga tcctttttct
    10801 acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt aaaacaggga
    10861 tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc accggctgtg
    10921 cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat tcgccttctt
    10981 cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca taaaattcga
    11041 tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt gttgcctcat
    11101 cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt tcaccgaata
    11161 attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct cgttgtgcta
    11221 ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga tcttccatat
    11281 aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg atcgatttca
    11341 ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga atatgttcga
    11401 tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct gtatcagtta
    11461 aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag cgacaagatt
    11521 gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat gtgcatcgac
    11581 cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta tccgtatttt
    11641 tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca cgttccaacc
    11701 cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga gtggataaat
    11761 ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga tcagaaacaa
    11821 tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct ccatcttcat
    11881 ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt tcttttgcaa
    11941 aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt cttaaaccat
    12001 atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt tttaactttt
    12061 ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt actgtcattt
    12121 ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc ctgtatctaa
    12181 ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg ctttttcttg
    12241 atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag tgatgtttac
    12301 gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat gatgctgata
    12361 aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat gtgtgccatg
    12421 actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga taaaaagacc
    12481 tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct gtagcagtgg
    12541 acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag gaatatcgag
    12601 tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta ctgctccaac
    12661 tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg tttcagcatc
    12721 ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa tacctgtatc
    12781 accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt caggtacatt
    12841 tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt taccatactt
    12901 gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag gcagacgcaa
    12961 tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca actgttctgc
    13021 ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct cctacaactt
    13081 cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa gtcggatttt
    13141 tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca aatataaaaa
    13201 gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt ataaaaattc
    13261 agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt ccgcgaacat
    13321 tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa cgaatttctc
    13381 cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg cgacggattg
    13441 gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt gcagatggtg
    13501 tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg ctcatgattt
    13561 ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc tcatcgattc
    13621 gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt ttttgcatgg
    13681 ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct tctcgaattt
    13741 gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc acttttctac
    13801 ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg aaaacgtctg
    13861 gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga cgaatttttt
    13921 ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg attaatcggc
    13981 ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca tcttcacttc
    14041 caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca gttggtccaa
    14101 ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt gcacggcgga
    14161 tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca tgcagaagtc
    14221 cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga gggacaccag
    14281 tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa taaccttgct
    14341 cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct ttttcttgtc
    14401 tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc ttttcttgta
    14461 ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg gtttgatcca
    14521 aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt agttgacgag
    14581 aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct gtgatctcca
    14641 ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct gcttcttcag
    14701 gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa tctttttcga
    14761 tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct cctcgagcta
    14821 acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct gcaccaatta
    14881 aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt tcgtcgatga
    14941 cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc aaagatccca
    15001 tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct aaaatgattt
    15061 tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc accagtacag
    15121 ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct ctcgaacgac
    15181 caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt gctaatgaat
    15241 ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca tttctacgac
    15301 gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc agaacttgac
    15361 gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg atactttcat
    15421 cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca gttttcttca
    15481 cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga agaaagactc
    15541 ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct tcataaattt
    15601 ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt tgctcgacca
    15661 ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt tttgcttctt
    15721 cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc atctaatctc
    15781 acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga gcacgtgtat
    15841 gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac atcaagtttc
    15901 cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca aaagcatttt
    15961 tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt tttttatctg
    16021 aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt tctactagat
    16081 aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa ggaacacagt
    16141 taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt tccagaattt
    16201 tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta ttcattccct
    16261 ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga agaacgcttt
    16321 ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta ttccgtatcg
    16381 acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca ctctatccag
    16441 ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa acaaaaagca
    16501 agataaaaaa aatcgggaag acaggattcg aacctgcgac cccttggtcc caaaccaagt
    16561 gctctaccaa gctgagctac ttcccgttta aaaaataaaa aagac
//
LOCUS       DASHFF010000054        16444 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.054, whole genome shotgun sequence.
ACCESSION   DASHFF010000054 DASHFF010000000
VERSION     DASHFF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.054"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..351
                     /locus_tag="U2I75_002028"
     CDS             <1..351
                     /locus_tag="U2I75_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1697132.1"
                     /translation="LKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPT
                     SGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVE
                     DSTLIEQIFNDITK"
     gene            369..938
                     /locus_tag="U2I75_002029"
     CDS             369..938
                     /locus_tag="U2I75_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN1697133.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            1048..2802
                     /locus_tag="U2I75_002030"
     CDS             1048..2802
                     /locus_tag="U2I75_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1697134.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            2762..4528
                     /locus_tag="U2I75_002031"
     CDS             2762..4528
                     /locus_tag="U2I75_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1697135.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            5051..5320
                     /gene="rpsN"
                     /locus_tag="U2I75_002032"
     CDS             5051..5320
                     /gene="rpsN"
                     /locus_tag="U2I75_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1697136.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            5335..5514
                     /gene="rpmF"
                     /locus_tag="U2I75_002033"
     CDS             5335..5514
                     /gene="rpmF"
                     /locus_tag="U2I75_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1697137.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            5546..5923
                     /locus_tag="U2I75_002034"
     CDS             5546..5923
                     /locus_tag="U2I75_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697138.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            5944..6093
                     /gene="rpmG"
                     /locus_tag="U2I75_002035"
     CDS             5944..6093
                     /gene="rpmG"
                     /locus_tag="U2I75_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1697139.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            6117..7100
                     /locus_tag="U2I75_002036"
     CDS             6117..7100
                     /locus_tag="U2I75_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN1697140.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            7130..7252
                     /locus_tag="U2I75_002037"
     CDS             7130..7252
                     /locus_tag="U2I75_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1697141.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            7270..8793
                     /locus_tag="U2I75_002038"
     CDS             7270..8793
                     /locus_tag="U2I75_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1697142.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            8817..9056
                     /locus_tag="U2I75_002039"
     CDS             8817..9056
                     /locus_tag="U2I75_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697143.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(9249..9710)
                     /locus_tag="U2I75_002040"
     CDS             complement(9249..9710)
                     /locus_tag="U2I75_002040"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1697144.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(9905..10123)
                     /locus_tag="U2I75_002041"
     CDS             complement(9905..10123)
                     /locus_tag="U2I75_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697145.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            10241..11857
                     /locus_tag="U2I75_002042"
     CDS             10241..11857
                     /locus_tag="U2I75_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1697146.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(12098..13438)
                     /locus_tag="U2I75_002043"
                     /pseudo
     CDS             complement(12098..13438)
                     /locus_tag="U2I75_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(13714..13869)
                     /locus_tag="U2I75_002044"
     CDS             complement(13714..13869)
                     /locus_tag="U2I75_002044"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN1697147.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(14165..14497)
                     /locus_tag="U2I75_002045"
     CDS             complement(14165..14497)
                     /locus_tag="U2I75_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1697148.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(15011..15202)
                     /locus_tag="U2I75_002046"
     CDS             complement(15011..15202)
                     /locus_tag="U2I75_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697149.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(15296..15682)
                     /locus_tag="U2I75_002047"
     CDS             complement(15296..15682)
                     /locus_tag="U2I75_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1697150.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         5514 a         2467 c         3128 g         5335 t
ORIGIN      
        1 ttaaaaaaaa tggaacttta taaaatgaaa gatcaattac cgtttgattt atccggtgga
       61 gaaaaacaaa gattattggt ttctatagct tcattgtcaa aaactaattt atttctattt
      121 gatgaaccta caagtgggtt ggattatatc aatatggaaa ggattgctga ccttattaaa
      181 gacttaaaag aaaagggtgc agtagttata gccactcatg atccggaatt tctttataaa
      241 acatgcaata gaattattta tttaaaaaat ggtggaataa aaaaagatat aaaacttaga
      301 gtagaagatt caactttgat agaacagatt tttaatgaca taacgaaata gaattgagga
      361 gaatatatat gaaaacaaaa gattatattt ttattggttt agctacagtt atttccctag
      421 taatttactt tttttcaata atggttagta gtattggtgg ggcgtttggt cacagcatat
      481 ccccagggat ttttggatta ttatctggaa tcatatttgt ttatattagc tataactacc
      541 ctagaaaagg aatttttact atttacacaa ttgttttatt gttatttttt acgttaatgg
      601 gcggggcgta cttaccttgg tttatcagtt cgattagtat ggcgatctta gctgatatta
      661 ttctatctgt atttggatat gatcgagcaa ttcctcaagt tgtgtcttgg gttctaatgc
      721 aactaggcag tgctgccgga cagtggattc ccatatggtt ctttacagat cgttttcgtc
      781 aagactggat agatagagga caatctgctg caacaatgga tgcaatgatc cactacgcgg
      841 tgggtatatg gggaattatt tcagtgttag ttgtagcaag tttatctatg attggtgtat
      901 tgattggaag aaaagtctta aaaaaataca aaaaatagaa cgtagtttac cttctatctg
      961 tttgtacatc tctaatttac gctattacgg aaagcattaa cagttgtaaa cgaataagag
     1021 aagaaattga atgcggggga ggaaaaaatg aaagtataca aaaaattatt tagttatgtc
     1081 cctaaaatga aatactacgg atggttagcc actttatttt ctgatttttc tgtcgtatta
     1141 acagtctatg gatattacag tatttacaaa tttcttcagt ctctaattat tgtaggagat
     1201 gaatatttag ctaagagtta tgcggttcaa actgttgctt ggttaacttt aggagcttta
     1261 ttttatattt tttctggctt attttctcat atccttgggt ttcgcttaga gactaatttg
     1321 agaaaaaagg gaattagcgg tttaacagaa gcaagttttc gtttttttga tttaaattct
     1381 tcgggatata ttcgaaagac aattgatgac aacgcggcta aaacacatat ggcaattgct
     1441 cacttgattc cagatagtgc tcaagccata gtaacacctg ttttatcagt tgccttaggc
     1501 tttttcatta actttaaaat tggattggtt ctaatcgcca tgttagtaat tggtgtattc
     1561 ttgttaaaga aaatgacaga gaatacaaat tttattcaaa aatatcaaga atcgttagat
     1621 gtgttaagct ctgaaacggt agaatatatc cgcggaattc aagttataaa gatatttggt
     1681 gctaaagtaa catcatttaa agccttgaat aaagcaatta acgattatgc taagtttgca
     1741 tataagtatt ctttgagtgg gaaaacacct tttgttcttt ttcaatggct attttttggc
     1801 atagtttgca ttttgatagt tcctataacc ttttttataa cttctcttac cactcccaag
     1861 atcttagctg ttgacttgtt gatgttgttt tttttaagtg gaattatttt tgtttctttc
     1921 atgagaataa tgtatgtaag tatgcacttg tttgaagcaa gatatgcagt cgaaacacta
     1981 gactcccttt atttagaaat gaagcaagac caactaaaat atgggaacga gacacagatt
     2041 aaaaattgtg acattgcatt tgaaaatgtc agcttttcat acaaagaaga gtgtgtttta
     2101 gaaaatttca acttagtttt agaacaaaaa agaatttatg cattagtagg aaaatcagga
     2161 agcggaaaga gtacgatagc taaactaatt tcaggtttct acaatgtgga taagggacag
     2221 attaaaattg gtggtaagcc tttaaatcac tattcaaaag acacaattat taacacaatt
     2281 gcttttgtct ttcaagatac gaaactattc aatcagagta tttacgataa cgtttctata
     2341 gcaaatcctc aagcgccagc aagtaaagta tttgacgcgc taagattagc cggttgcgaa
     2401 tcgattttag aaaaatttcc cgaaagagag cataccttaa ttggtagtaa aggagtttat
     2461 ctgtcaggag gagaaaaaca acgtatagct attgccagag caattttaaa agatgcaaag
     2521 attgttatta tggatgaagc cagtgcatct attgatccag ataatgagta tgaactacaa
     2581 aaggcttttc aatatttgat gaaagaaaaa acagttatta tgatagctca tcgattatcc
     2641 agcattaaat ctgtagatga aattcttgtc ttgtcagaag gaaaaatagt agaacgtgga
     2701 aatcataatg aattaatggc taaacatcga gtgtataaac aattggtcac taattatgag
     2761 aatgcaaatg attggaggct tgacaatgaa aaatttttat aaaaaaaaat ttgctttaac
     2821 tgatcagggc gcagaagctt taactaaagc ttctatttct agtttttttg tatactgcat
     2881 taatatggta ccagcattca ttataatgat gctgatagac gaattagttt tagaaaatgc
     2941 aaagccccgt tggctttatt ttgcagtatc gtttgttact ttgcttttca tgtattggtt
     3001 gttagatagg gagtatgaaa acttatataa tagtacttat aaagaaagtg cgcatttaag
     3061 agtgcaaatt gcagacgatt tgtcaaattt accattatcc tatttttcaa aacataattt
     3121 atcagattta tctcaaacta tcatgtctga cgttgaaggt attgagcatg cgatgagtca
     3181 tgcaatacct aaagccggcg gtatggctct gtttttccct tttatttcag tgatgctttt
     3241 ggttggtaat gtcaaaatgg gattagctgt tattttgcca acgttattta gttttgtctt
     3301 aatcttgtta tcaaagaaat cccaaacgaa agccaatact aaatattacg atactttgag
     3361 agaaaactcg gaagaatttc aagaaactat tgaattgcag caagagatta atagctttaa
     3421 tctatctaaa aaagttcaag acagactttt caaaaaaatg gaagagagtg aaaggattca
     3481 tttaaaggta gaattaagta ctttttcagt catggcctta tcctctattt tctcatatgt
     3541 tagtttagca gtagtcattc tagtaggtgt tcacttactg ttaacgggtg aagtgactat
     3601 actctacgtc gttggttact tactagccgc aataaaaata aaggattcct tcgattctat
     3661 gaaagaagca gtgcttgaaa tattttattt agctcctaaa atacagcgaa ttagagctat
     3721 gaaggaaacc agtatccaag agggatcaga tagtcctctg aaatcttttg acattgagtt
     3781 gcgagatgtc tctttctcat atgataacaa taccccaatt ttagatcata tctcttttac
     3841 ggctaaacaa ggagaagtga ctgcgttagt aggtgctagt ggttccggga aaactagtat
     3901 cttgaaatta gtgtctagac tgtatgatta tgatgaaggc tgtattttaa ttgatgggta
     3961 tgatattaaa cgtgtatctc cagcttcttt gtttagcaaa atcgctattg tttttcaaga
     4021 ggtaactttg tttaatacaa gtattttgga aaatatacgt attggaaata gtcaagcgtc
     4081 agatgaggaa gtgaaaaaag cagctcgttt agctaactgc gaagacttta ttgaaaaact
     4141 acctgacggt tatcatacat tggtcggaga aaacggaagt tcactttctg gaggagagcg
     4201 acaacgccta tccatagcac gggctttttt gaaaaatgcg ccaattttaa ttttagatga
     4261 aattactgcc agtttagatg cggaaaatga aaaacgtatt caggaaagtt taaatcgttt
     4321 aatacaagat aagacagtac taattatatc gcatcgcttg aagtctattg aaaaggtaaa
     4381 taaaattgta gtgatggatc aaggaaaact agttgatcaa ggtactcata atgagttgta
     4441 tcgaaggtca gaaatataca aaaatctcat aaagaaaact aaactatctg agaaattcgt
     4501 ctatgaaaaa gaagctcaat cacgatgatg aaataggctt taaattaacc tacaattttt
     4561 gggatatagt tataattctt tttcaaaaag aataatttta ttaaacagca tctaattttg
     4621 aatacacgaa agaatcagta tgattgaatt agttcactta atgtctattc ttataatata
     4681 aggaataatc aaagttttaa aaaattggcg ataaaccagt taaacataaa agattgaata
     4741 aaaagttata gtttctttca tattgccgtt ttaagtagag gaaaagctac ttaaaacggt
     4801 atttgtgttt gattggacta ataaaagtga attcttattt ttcagagcct aaactgcata
     4861 ttcttttaaa tgatttgatt atctctatgt accgataaat tcgttttcaa atagttatct
     4921 attcatatga gttttatttc atgttattta ttgttgacaa gagcaactct tgttggtaaa
     4981 gtatcagtgt caaaacgtaa cgattacgat ttgaggtgtc taaatcaaaa taaaaggagt
     5041 ataaaggtat atggcaaaaa agtcgaaaat tgctaaagca aagaaacaaa tggcgatgat
     5101 tgaaaagtat gcagataaac gtcaggaatt aaaagccgca ggagatcgca ccgctctcgc
     5161 taaattgcca agagattcaa atccgaatcg tctgaggctt cgtgatcaga cggatggtcg
     5221 tccaagaggg tatatgcgaa agttcggtat gtcccgtatt aagtttagag aacttgccca
     5281 tcaagggttg attcctggag taaaaaaagc aagttggtaa aaggaggaaa tatcatggca
     5341 gtaccagcaa gaaaaacatc taaggcgaag aaacggttaa gaagaactca tcaaactgta
     5401 gtaaaaccag aaatttcatt tgacgaagcg aatggtgatt atagacgtag ccatcatgta
     5461 tctttaaaag ggtactataa tggaaagaaa gtaattaaag gagcttcaaa atagtttaat
     5521 atataacttt taggagggaa tcaaaatgga aattatttac ccacctttag ttgaagaagg
     5581 attaaagtat tatcttgaaa cgacccagca atcgctagat aaatgcactt tttatcgttc
     5641 gatggtagag agaggaatta ttactgaaac gggtttaccg acccaacagg caattgaaaa
     5701 tggattagtc aaagattact atgaggacca aggcttatca tttgatgatt ttttaagaat
     5761 ctatccaatt tttgaagagt atgacgagga gttatttcaa tgtatcgatg gttattggga
     5821 gatacctgtt gatatgaaag aaaatttagt ttcacaattg gaatctggcg agttaacttt
     5881 tgaagatgca caacaaattc aagcatatct tgaagatcga taagtattga aggaggaaga
     5941 tttatgcgtc aaaatattat tttggaatgt gtcgaaactg gagaacgtct gtatctcacc
     6001 agcaaaaata aacggaataa ccctgaacgg ttagaattaa aaaagtattc gccaaaattg
     6061 cgcagacggg caatttttaa agaagtgaaa tagctaagtg gtaaaaaggt gacaaaatgg
     6121 gcataccagt tacaatcgtt tctgggtttt taggggctgg aaaaacaacg ctaatcaatc
     6181 aagccttgca agtctcaccc taccccaggg aagaaattat tataattgaa aatgagtttg
     6241 gtgaagttgg gattgatcac aaattattag ttcatagcga ggaacgggtg ttacaactaa
     6301 ataatggatg tatgtgttgc agtcttcgca gtgatttatt agcaatattg atttctttac
     6361 tggaaacaat agaagaacat caacaaccaa tttcacaaat catcattgaa actacaggta
     6421 tagcagatcc ccaaccaatc attcaaacac ttttaacagc gcctcaacta agaggaagag
     6481 tatatatcga tagtttattg actgttattg atagtgacag ttttgaactt attgaaagag
     6541 aaccacaagc ccttaagcaa cttgtcatgg cagatcgcat ttttgtgtct agaaaacaaa
     6601 acttgaattt atctgcacta aaaaaattac agagagaaat caaaagtatt aaccctttat
     6661 ccgatattcg aatgttcaca catagtgaaa cgattcaaaa aaaggatttt tataatttga
     6721 aaaaatttaa tcatccctta gtttttgaaa gtgattcatc agaaaatcac tataatggca
     6781 aatactatca tcaacaccat caagtacatg agtttaaatc aattctctta acttcagatc
     6841 aagacataaa aaaagatttt ttactccaat ggattgactg gctgatttta accaaccaag
     6901 ggagcctgta tcgttttaaa ggattgattg ctttacaaga gcaggatttt atagttgcca
     6961 tgcaagaggt aaacgaacag gttaattttc aatacaccac tcgacaaaaa gaccaaacca
     7021 ctattattct aatcggaaaa atgttggatg aagaaaggat caaacagagt tttgataaat
     7081 tactggcgga aagcaaataa tgataagtag tgattggggg taatttctaa tggagaaaac
     7141 ggatatatct agtgcatatc gacgacttaa aagcccgaat attaaaacta gaaaaagggc
     7201 attaaaaatt atcaaagaac ataaacgaaa taaaatgaag aaattagcat aattaaggaa
     7261 ggaaaaagaa tgaagaaaat cagtgtagga cttattggga tagcagctct aggattattg
     7321 ggagcttgct ccagcactaa tgatgcaaaa gtatcgaatg ataaagatgg aaaattagaa
     7381 attgtaacaa cattctatcc aatgtatgat ttcaccaaaa acatcgtcgg cgatgaagcg
     7441 aacgtggatt taatggttcc agcgggatca gaacctcatg actatgagcc ctcagctaag
     7501 gatatggcaa aagctcatga tgctgatgtt tttgtatatc ataacgaaaa catggaatca
     7561 tgggtaccaa aagctaaaga aagttggaag aaagctgggc ctaatgtagt agaaggaaca
     7621 aaagacatga ttttattgcc aggtagtgaa gaagaagatc atgatcacgg agaagaagat
     7681 caccatcatg aactagatcc tcatacgtgg gtttcaccca aaatggcaat taaggaagtt
     7741 tcaaatatca aggatcagtt agttaaattg tatccgaaaa aagcaaaagt atttgagaca
     7801 aatgctgaaa agtatttgac taaactgaaa cggttggatg ctgactatac cactagtctt
     7861 aaagaagcga aacaaaaaag ttttgtgacg caacacgcag cctttggata tttagctttg
     7921 gattatggat tgaaacaagt tcctattgct ggactaagtc ctgaagaaga gccatcttct
     7981 ggtcgattag ctgaattaaa agagtatgtt aaaaaaaata agatcaatta tatttacttt
     8041 gaaaagaatg ctaatgataa aattgcaaaa actttggcca acgaagctgg gataaaattg
     8101 gaagtactga atcctcttga aagtcttaca aaagaacaaa tggataatgg tgaagattat
     8161 gtttcagtaa tggaagacaa tctaaaagcg ttagaaaaaa caactatggt tgctggtgaa
     8221 gaggtagttc cagaaaaaga agcgaaggat gaaaagacag ttgctaatgg gtactttaaa
     8281 gatgccgatg tgaaggatcg cgaattgagt gattatacag gcgaatggca atctgtttat
     8341 ccgttgttaa aagatggaat tttggatgaa gttttcgatt acaaagcaaa aatcaataaa
     8401 gacatgacag ctgccgagta caaagattat tatactactg gctataagac agatattgat
     8461 actatcaaca ttaaagacaa caccattgat tttgtagtca acggtgaaca tcatcaatac
     8521 acatacaaat atgttggata caaaattttg aattatgaaa aaggaaaccg tggtgttcgt
     8581 tttaattttg aaactgatga tgcaggtgct ggtcgtttta aatatattca atttagtgat
     8641 catggaattg caccaagtaa ggcagagcat ttccatattt tctttggtgg agaaagtcaa
     8701 gaaaaactct ataatgaatt gcataattgg cctactttct atccagcttc attgtctaaa
     8761 catgaaattg ctcaagaaat gatggctcat taaaattcaa tttttggagg aaatacatgg
     8821 taagaaaagt attattgtct atggattcca agaatattga tgcttatcgg cgttatcgat
     8881 ttgcaaagca aatgagatta aatgggagta cattagctga tattgctgca actttacatg
     8941 ttagtattga cacagttcat agagtattaa aacgggatcc tgattgttgt tttatcaagg
     9001 tcaatactgg aagaagtaga cgtttagcaa aacggcataa tagcttggcc aattaattga
     9061 taataccaga gtagatcatt tttcccttat ttggatttga tctgcttttt attatgtctt
     9121 gaattgtcaa atcaagcgca acactttatc aaagaagact tcataaggtg ttctccaatc
     9181 taaacacttt ctaggtctgt tattcagttt ttcaatcatc tgatttatcg ttgagttatc
     9241 taccgtagct atctctttcg attttggcaa gtattctcga atgagtctat tcgtatttta
     9301 atttgttcat tgttgccaag ggcatgagga tctggaaaat aaaagggcat attattcaat
     9361 gcttctgtaa ctgcgaaatg attggagaat tcttttcctc tatctggagt gatcgttttg
     9421 accgcctcat ttggaatact gcttaaaagt tcaatcattt tctcgctcac ataagcggct
     9481 ttctttctgg ggaccttctt tcctgctaaa aggaagcatg attttctgtc tgtcaaagtc
     9541 actaaacaag taccaccagt tttgccagct acaatatcgg ctttccaatg accaaaatca
     9601 cttctatcat ctgcggattt tggacggcca tggatcgaat gagtaaggca aatctttcca
     9661 cgagactcga catgattttt tgtatgtctt gtttttcctc gatggtgcaa tgaacggatc
     9721 aggcctttat tgccgtgtga gaggggttct gtatcgaaaa gatcattgca aattgcacgt
     9781 tagatcgtga taaaactaat tgaaaagtcc atatgttcat gcttcaaacg attcgagatt
     9841 tcctctggcg accactgaaa atcgacaaat aaatgacgaa caattgacca aactcacgga
     9901 tcatttagaa tgtgataacg tccgcatttt tttcttcgat aagcataatt tttttccgct
     9961 ttggaaggtg aatagttttt ttcttgtttc ttctcagttc atgtaaaata gtaaacgggc
    10021 tatgctgaat gtcgttgcca attatactaa tagaaaatcc ttcttcgtga agcagaaaga
    10081 ttctttctcg ttcgagtata gtaagatgac gatactggct cataattatt ttccttctag
    10141 gagtgttctc gacaaactta tactagttgg atatcgatta tgactcattt ttatgtgttg
    10201 cacttaaagt gtaaattcaa ggtatagaaa gaggttccct atgaaaaaaa tacctattac
    10261 aatcttaact ggctatcttg gttcaggaaa aacgatactt ctaaatcata ttctgtacgg
    10321 tgatcacgga aaggaaattg ctgtcattgt aaatgacata ggggaagtaa atgttgatgg
    10381 ggtattagtc gacaagaaag gttttaaaag aactggagaa aaacttgttt cgatgccgaa
    10441 tggatgcatt tgttgtacat tacgcgaaga tttgattgtt gaactagatc aattagtgca
    10501 attttctgac ttgaatcaaa ttgttattga agcttcagga atcagcaaac caattccaat
    10561 tattcaagca attatattag ctaaaaaccc tttagacatt gatttgaccc aacgattaga
    10621 gattggtgcc attattatag tagtggatgt aaaacgtttt tgggataatt tcgaaacggg
    10681 tgaaaagatt caggaaagaa aagctgaagg taatgtagtt gaagaagaac aggatattcg
    10741 tggcttgtta attgatcaat tcgagtgttg taacatatta cttttaaata atagtgactt
    10801 agtgtcaacc aagacgattt acaaaattaa cggtctaatt gctaagttac agtccaatgc
    10861 caaaataatt aggacaatta aaggtgaagt agacttatcg aaaattctaa atacaaatct
    10921 ttttaatata gatgagactt cctattcagt gggctggttc aaagagttag agcttggcta
    10981 tcaaaatcat attcctgaaa cagaagaata tggtatctca agttttatct atcgaaatcg
    11041 gttactcctt gacgcacaaa aatttgccca tcttacggat aaaaattttc ctggcaatat
    11101 tacaagagca aaagggtttc tttggtttgc ccaacatagt gatttttcga cgatctttga
    11161 aacatcgggt agctataaaa gtattgaatc attaaattat tgggtagcta gtttacctaa
    11221 aattgagtgt caaatgatta tacaaaaaaa tcccgaattg aaggaagcct ggcatccgtt
    11281 gtttggtgaa cgcattaata aactagtttt tattggtatg aagatggatc aacaatgtat
    11341 taaggaacaa cttgagggtt gtttaactga ttctcttaat gcagataatt tccgaaaaat
    11401 ttcaccattt ccatggaaaa agtctaatat gtttagccag atatcgttaa tgaaattttt
    11461 aagaaaagct ggtgtcaaga taggcgatgt cataattatt ggcattttga ttattacctc
    11521 ttttattccc ttatttgtat tctctataga aaaggagaat gaaacaactg aagatgctat
    11581 caaatatgcc atcgttcgaa ttgatggcaa ggaaatggat cgcttcgatt tggataagat
    11641 caatcataaa ctcgtgaatt atcatcctgc gaagggacaa tataatattg ttgagattaa
    11701 agatggaaga attcgagtta aagaagataa tagtcctgat caaatcgcgg ttagaactgg
    11761 atggatctca aaacccggtc aaacgagtat ttgtttaccc cataagttag ttattagtat
    11821 tgagaaaaaa gaaagcacag attactacat ttactaatct ggaaatagat gaggaggttt
    11881 ttaataagaa aaagatatta ttattaataa tagcaggggc tttattaagt gtgttaagcg
    11941 cttgtggaca agaaaaagaa gcggtgacaa caggcacaag tatctttaac gggacctgca
    12001 atggtttagt taacagtcgg ttagattact tattttggct cttcgtcaat gagtgttggt
    12061 gaagaaattc aatagaataa tcgagatact cacacggcta gacagctatc ttgtctagcc
    12121 gtgttgttgt tctggcttta gtcagttggg tagtattctt agttttctta aaatattttc
    12181 ctcgatataa aaagattcgt tctcggtagt tttgaaagtt tctatagcca taggcaattc
    12241 gttttagtac tttgatatgg ttattcaaac attctatagg gccgttggaa tagggaacgc
    12301 gcaacgctcg tttgatttct gtttggtatt tcttgaaggt cgtgaacact ggttggtagt
    12361 gctctggcaa ctccttgtag tcttctttta ggagcgcgac gaattcagga acatcttgtc
    12421 ttctgataac ggaaagaaaa tcctggtaga cttcgtagcc tcgctttaag gaatcatcat
    12481 aagataatag ccgatcgact atctctgctt ctgtcagatg atctcgaaat gacggacgcc
    12541 agtagcgatg ctgacctttc aattcccacg cattcttttg aagtagtttc cagtatttct
    12601 ttaagtgctt cgaccgacga tccccttttt taaaggtctt cattacttga attcggtggt
    12661 tggtaaaagc ccgaccaatg tgttggctaa tatgaaagcg atcggcgacg attcgggcat
    12721 tcggaaaaca ctctttcacc aatgtgcgat agggtgtata gaaatctgaa accactagac
    12781 gcacttgttc acggactttt atagggtagc gcaagaaata gtcgcgtaaa ggattgagtt
    12841 gccgattttc cacaatatca atcagttgat gggtcttgcc gtccatcatg acaaagctca
    12901 tggctcccga gacatttttc actgatttga attcatcgaa acacaccact tctggtaaca
    12961 gctggctcaa agaaatggtt ttaggccgaa ggctgcgaag tattcgaatg acagtggtcg
    13021 gagaaatgtg cttcatacgt gcgatcaaag acatagaaag aggttcgctt aatagctcaa
    13081 gaatggcttg tttgactctg cgagcaatgg agcagcgacg atcactaact gaatcttctg
    13141 ctaaaaaagt ctttccacag tgtttacatt tgaagcgctg tttgttaatc cgcagaatgg
    13201 tcttgtagtt actgacatca ttcaataaga tggatacttt tttccagccc tatttaatga
    13261 tcgtttgttc attttgatag tggcgatgaa agcattcctc cggttgatag gtcaatgtgc
    13321 catcaaaaac aaaacatgtt tcgtcattga ttcgctcttt gcgaaaggca tcttcatgaa
    13381 agataagatt taggtctaga atatctagta actctttggt aaactctgaa taggacatct
    13441 tgaaacactc ctttgaatgg ttttcgtcga ctttattcta aaggatttta aggtgtctgt
    13501 ttgcgtttat tagaaaaaag aaaaagtatt ggcaacagaa ggcgtcacca acactagata
    13561 ttatagatcc cttattttct tcgcttcaaa ttgtcaagga gtcatcataa attttgtatg
    13621 gattcttttg tttgggtagt agtcttctaa ataatcttgg attaacacct tcatctctat
    13681 tttactttta tatttgttgg agtcgattaa ttctcatttg attgatcgat gaaacgcttc
    13741 aattactgca ttgtcaacag gagtcccagt ttggctcact gaatgtgtga tgtggtggtc
    13801 gttcaacagc tcttgataaa taaaagaccg atgctggcta cctctatcgg aatgaattac
    13861 cgtaggcaat tctccttaat aaatttgtaa tggacggacg acaatctctt ttttcatgtg
    13921 tgaatctacc atatagtaac gtagaatacg agttgctaag tcaatatagg tggacaaata
    13981 gaggcgaccg tcattacatg gaatgtaagt aatatccatc acccaaacag catcaatttg
    14041 gttctgttca aattatcgat tcaacatttt tttaccattt ctttgattgt tttttctcta
    14101 ccatgagaag atttgcgtcg atgaaatcct cttgtgcata aattcatttc acgcataaga
    14161 acagctacaa cacgtttatt gatttcgatt ccttcatcgt aaagtttttg agtgatccct
    14221 ggagatccgt atcgatcttt gtgttcgtag aacactcgtc ttcctctttt agtcgtttat
    14281 tttcctgttc aagaatgtag aaatctgctt tatcaggtaa tctttttcct tgtccagcaa
    14341 aagcattttt cccgtatcga cgatattctg aaacccatcg ggaaattgtt ggacgagaga
    14401 tattgtattc ttcagcaatt ttcttggtag ttactttttt ctcaaggata tcctgtgaaa
    14461 tctttagttt aaatttacga tcatatgttt ttctcaatag agttcattcc ttccggtaac
    14521 gtaactgata gtcaagaaaa gtgtatcata gcccaactta gtatcagggt ttgtcaacta
    14581 aactgtggaa gttgatagat aagagttttt aacaaccaaa attctcttga ctatttttga
    14641 aatcagacaa attttgattg gacacatagt tgaataaatc taccgtcgtt acggaaggta
    14701 aatcgcatgc ttttcaattc tagaggagaa gtcctcgttg aagtaccata gcccagttct
    14761 ggattcgacc tcctgcccac ttcatacgta aataaagtaa ttttaggagt gagactgtga
    14821 caaaagttat ttttaagcta aaaacgaatt aaaaaacgtt gataattaaa atcattcgtt
    14881 ttagattcta tgaatcatta tagaacgctg taaaatcgat actttataat tataaagaag
    14941 atagtttcaa atttattgtt cgaaatttga aactatcttt ttacttatgt cacagactcc
    15001 cttagttgag ttattcttta atttcgaatt tcgagatagc tagatatatt gcaagcatac
    15061 attcttgata acaatcttga tcatattttc cagttctcca ggattgctta tgaatcaaag
    15121 agctaaatct tttgagaatt gcctcaacag cttcttcatt tccagccttt gctaaaatga
    15181 aatcttcttt taaagaattc ataaatctac cttcttccca tggacttata atctatttct
    15241 ttgggaagga ggagaacgag caaagaagca tacaaaatta gttattatgc agatattaaa
    15301 ccagcatttt ttgaattttt ttgtaaagtc gatgttttaa atttgtcgca ccctgtcttg
    15361 taatccccag aacctttcca atttctttat cagttttatc aaagataaat ttttcgataa
    15421 gaaaaatttg ttcaggttct ttcaacaaag gaaggatttt tgctaatttt atatcttcaa
    15481 caaggagagt cacatttaaa acattaatcg gttgtttaaa ttttatagta tctttagtaa
    15541 acaaatatgg tgggagttga tcaagttgtt catgtttttg aatttcttct tgttttttat
    15601 aataagtatt agcggtgttc aaaatgacct tttcaaagaa tcgaatcaac tgggcattta
    15661 cgcgttcgtc gttatctgac atattgcaca cctcctccat taagatttaa agggaaaaga
    15721 ggcaaaaaac acgaaaagaa gctaatgcaa ttatgctgcg ctgcgaataa cttctataag
    15781 ctcacatgca ttgataggac tgctgatgtt tattttcaaa aatgtttttt tagttgataa
    15841 tggatagtta tctttatact tagaaaagaa aattcatata tcttcttaaa tatcaaacat
    15901 gtccttatgg ctaagaaaat gtcttgagct ttttacgtat aataaatctt ttctaaaacc
    15961 tccttttgga taatattctt attcgtgtat agattgtatt ttttatttct tcccccttca
    16021 taattaacaa ctagaaaaga gttcgaatag acacgtattt gaacgaaaat ttttattgga
    16081 ttcccaaatt ggaatattta gaaacgaaaa attcctatta tctgtatagt gcttagataa
    16141 taacggatca taataattat tgtctttgaa ctagatatat gtaaatagta ttcatcatga
    16201 tgaaaaatga tgagttctcg gtgataaaat gtacttttta cggtggaata tacatgaaag
    16261 cgataagtgg atattttata atgaagtaag ttagcttatt ttgacagagg aatagtattt
    16321 tagataacgt ataaaatact atttaatgat gtagaaagga aagagagaaa caaaagtgtt
    16381 ttcagataaa gaagtcttat taaagtctct tcatgtttgt ttaaagttac ctatcgtggt
    16441 tttt
//
LOCUS       DASHFF010000055        16050 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.055, whole genome shotgun sequence.
ACCESSION   DASHFF010000055 DASHFF010000000
VERSION     DASHFF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.055"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            172..516
                     /locus_tag="U2I75_002048"
     CDS             172..516
                     /locus_tag="U2I75_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN1697151.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="U2I75_002049"
     CDS             517..936
                     /locus_tag="U2I75_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEN1697152.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="U2I75_002050"
     CDS             complement(1028..1378)
                     /locus_tag="U2I75_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697153.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="U2I75_002051"
     CDS             complement(1344..2675)
                     /locus_tag="U2I75_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN1697154.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="U2I75_002052"
     CDS             2930..3496
                     /locus_tag="U2I75_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1697155.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="U2I75_002053"
     tRNA            3641..3728
                     /locus_tag="U2I75_002053"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4064..5038
                     /gene="bsh"
                     /locus_tag="U2I75_002054"
     CDS             4064..5038
                     /gene="bsh"
                     /locus_tag="U2I75_002054"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN1697156.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            5363..7258
                     /gene="asnB"
                     /locus_tag="U2I75_002055"
     CDS             5363..7258
                     /gene="asnB"
                     /locus_tag="U2I75_002055"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN1697157.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7453..7899)
                     /locus_tag="U2I75_002056"
     CDS             complement(7453..7899)
                     /locus_tag="U2I75_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN1697158.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8127..8276
                     /gene="rpmG"
                     /locus_tag="U2I75_002057"
     CDS             8127..8276
                     /gene="rpmG"
                     /locus_tag="U2I75_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1697159.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8364..8882
                     /locus_tag="U2I75_002058"
     CDS             8364..8882
                     /locus_tag="U2I75_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1697160.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8959..9915)
                     /locus_tag="U2I75_002059"
     CDS             complement(8959..9915)
                     /locus_tag="U2I75_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1697161.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10081..10887
                     /locus_tag="U2I75_002060"
     CDS             10081..10887
                     /locus_tag="U2I75_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1697162.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10884..11873
                     /locus_tag="U2I75_002061"
     CDS             10884..11873
                     /locus_tag="U2I75_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1697163.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11870..12874
                     /locus_tag="U2I75_002062"
     CDS             11870..12874
                     /locus_tag="U2I75_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1697164.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13006..13548
                     /locus_tag="U2I75_002063"
     CDS             13006..13548
                     /locus_tag="U2I75_002063"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN1697165.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13568..14266
                     /locus_tag="U2I75_002064"
     CDS             13568..14266
                     /locus_tag="U2I75_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN1697166.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14287..14499
                     /locus_tag="U2I75_002065"
     CDS             14287..14499
                     /locus_tag="U2I75_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN1697167.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14499..15461
                     /locus_tag="U2I75_002066"
     CDS             14499..15461
                     /locus_tag="U2I75_002066"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN1697168.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15479..15874
                     /locus_tag="U2I75_002067"
     CDS             15479..15874
                     /locus_tag="U2I75_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1697169.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
BASE COUNT         5142 a         2702 c         3308 g         4898 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattaaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg tagaatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga tgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc atacagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat agtagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagcacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcaagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cccatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc gaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtataccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataata cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaaa cacctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttgg ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc cttttttttg ataaaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggagggaaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtagtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaacaccag aaaatcattt ttcaaaccag atcagcttga atgtttatag
     4681 tcgtgggatg ggaggattgg gcttacctgg agatctctct tctgtttctc gatttgtaaa
     4741 agccacattt actaaaatga atgctgcatc aggtgattca gaatctgaaa gtatcagtca
     4801 attttttcat atactaggct cagtcgagca acaaaaagga gtatgcgata ctggagaagg
     4861 aaaatacgaa tatacgattt attcttcttg ctgtaacgtt gataaaggga tttattatta
     4921 tcgtacgtat gaagacagtc aaattactgc tgtaaatata aataaagaag acttggatcg
     4981 tcatgaattg gtcagttatc cagtcataaa agagcaacag ataaactatg tcaattaatt
     5041 aatgaataga tccgtttggt atcatatttt ctgatacagt cataattaga cacgatcttt
     5101 aggtcgtgtc tttttttgct gttttttttg gaaatagcat ccactaatat ttttttaaca
     5161 cgaagagaat aaggtagcct caaaaaagaa aacatggtaa attagatatt gggactttat
     5221 tttctgtaaa aacagaaact ttcatctttt gttttcacgt gacgaaattg taagagagaa
     5281 aatattcgtc cgctatttat tctatgataa gatgcatatg aatgaaataa acagtaattt
     5341 tatattagga tgtgaaagtt aaatgtgcgg tattgtaggt tttgttaacg acaaggacaa
     5401 caaaaaaaca attattaata caatgatgga tcgaatcgtt caccgtggac ctaatagttc
     5461 aggagaatat atcgataaac atgttgcttt aggattcaga agattaagta ttattgactt
     5521 agagggcggt actcagccta tttacaatga agatcggaca aagatcatta tttttaatgg
     5581 cgaaatctat aattaccagc ctttgagaga agaattgatt gcagctggcc acgtttttca
     5641 aacacatgca gatacagaag tccttttgca tggttacgaa gaatggggaa cagaattatt
     5701 gcaaaagatc cgcggaatgt ttgcttttgc tatttgggat aatgaaaaga atgagttatt
     5761 cggtgcaaga gaccactttg ggatcaaacc atattattat gcagaaatga acggaacatt
     5821 tatgtttggt tcagaaatca aaagttttct gcctcatcct gattttaaca aagaattgaa
     5881 caaagaagcg ttgaaacctt atatgacttt ccaatattct ccattaaacg gcgaaacatt
     5941 cttcaaagat gtctatcgtt tgcctgaagg ccattattat acgtataaag atggaaaact
     6001 agacattcag caatattggg atgcagattt tgaaacaaaa gaaacgcatt cccgacaaga
     6061 atggatcgaa aaaatcgacg aaaccgttca agcttctatt gaagcacata cggtcagtga
     6121 tgtagaagtt ggttctttct tatccagcgg ggttgattcc agttatgtaa cctctgtatt
     6181 gaaaccagat cattctttct caatcgggtt tgatgataaa acctataatg aagcgattga
     6241 agccagaaaa ctaaccgaac tattggactt agataataca gcagcagtga tcgacggaga
     6301 catgtcgttc aaagcattcc ctttgatcca gtatcactta gatgaacctg attcaaatcc
     6361 ttcttgcgta cctttgtact ttttagcaaa tcttgcttca caaagtgtgc gggtagtcca
     6421 atctggagaa ggggcggatg aactgtttgc gggataccaa acgtatggat tccatacgaa
     6481 ttcaaaattc atccgtgtga ttgctcaagg attgaaaaaa ttaccaaaag gaacacgcta
     6541 taatttagga cgtaaaatcg gaaaaatgaa aaatttccat ggacggatcc atttatatga
     6601 atcacttgct cctgcaaaag aatattttat cggtcatgct cgagtgttcg aagaatcaga
     6661 agcatcagaa gtgttaacac caaaatatca aacagcacca tctgtggatg aaatcatgac
     6721 tgttcattac gagaaaacag aaggaatcaa agatgaagtc aataagatgc aatatgtcga
     6781 tcttcatcaa tggatgccaa aagatatctt gttaaaggca gataaacttt ctatggcaag
     6841 ttctttggaa gtccgagtac ctttattgga tatcgaagtc atgaaattgg ctcaacaaat
     6901 accaagtaag tttttattga atcaaaacaa cacaaaagat attttcagac aagctgcgaa
     6961 caaacatctt ccggaagaat ggtcgaatcg tgtgaaatta ggattccctg tgccaatcaa
     7021 agcttggttg aaagaagaac acggctacga acaagtcaaa gcattattcg aagcagattt
     7081 tgcaaaagaa ttttttgatc aagaaaaaat catgaagttg ctagatgacc atcacgaagg
     7141 acgtaaagaa gagcaaagaa aaatctggac aatcttcagt ttcttgacat ggtataaagt
     7201 ctactttgta gatgaaacga ttccgcaagc agaagcaatc gactatgtga ctgtttgatg
     7261 aaaatcaaat atcaataaaa aagaggtttt ctcctaccgt ttaacactga gtgataagaa
     7321 acaatttcaa aaaacagctc ttcccgtttt atagatatta tgacttatgg ttcggagttg
     7381 gcagaggaac accattgttt cgaacataag ggggttgtga cacgattttc gtcacaaccc
     7441 ctttacatat gttcaataat cgatttcttt ttcttttgtc ccatcttttt tataaatgaa
     7501 caaagttgat tctttgtttt gtgctacttg ctttcctttt tcgattgctt ctttttttgt
     7561 atcaaaatgg ttgcttgcct tttttgcccc tttagatttc acgatccatt gttcttcctc
     7621 atattctacg atcacatctg aatcaagcag ttttccagca cgaggattcg tatcgtgttc
     7681 atccgtcttg cttggatttt tttctttttc gaaagttcgt ttttctgatt cactagcatc
     7741 ttcataccat ttttttgctt gatcaatggc aattgggatc gcacggttat cagggtaccc
     7801 ttcatctaat aatgcatttg cgatgtcgat tgcttttttt cttgtcaatt tatctaagtt
     7861 tttcattgat gcaggaaagt ccttcatgtt ccatggcata ttagtatccc cctttattaa
     7921 aaaacataat ctatataaac aagtgtaagg gtatttatac aaaaagaaaa agattttgtt
     7981 tgagaagtaa aaccgttgta tttatcgtca agtagtttta cttaacaaaa tcgtgaaaaa
     8041 cactagcaat ctagaaaaaa tcatggtaag ataatcaagt ctgagagtca tcttagagga
     8101 ttcttgtaaa aatgggaggg aaaaacatgc gcgtaaacat cactttagag tgtacttctt
     8161 gtaaagaacg taactatcta acaagcaaaa acaaacgtaa caatcctgat cgtttggaaa
     8221 aacaaaaata ttgcccacgc gaacgcaaag ttactttgca ccgtgaaaca aaataatgtt
     8281 tttaaagttc tcaattcctt gctggtcaaa gaattgagac tttttttatc tcaaaacatc
     8341 taaaatcata aggagcggat aatatgttta tttccatgtg ggcacaagat aaaaatggtc
     8401 tgatcggcaa aaatggtttg ttaccatggc gattgccaaa tgatatgcgt ttctttcgcg
     8461 aacatacaat ggatcgaatt ttagtgatgg gaagaaagac ctatgaagga atgggggatc
     8521 tttcacttcc ttatcgccat atcatcgtgt tgacaacgca accaaatttt cgcacgaaag
     8581 aaaatgcgga agtaatgcat tcgatcgatg aacttttgtc gtatagtcaa acgatctcag
     8641 aagatatcta tgtttcgggt ggtagtcgga tttttcaaga acttcttcct tacactggtc
     8701 ggatctggcg aacattgatc gatagcacgt ttgaaggcga tacgtatatt gggaatattg
     8761 atttttcaga cttcgccctt atagaggagt atgaggggat aacggatcaa gaaaatctat
     8821 atgcccatcg ttttcaaaaa tgggaaagag taaaaaatag ggttgcttca aaagaagaat
     8881 aaaatctatg cgtctagttt gttaataaga aaaaaagcat ctcacgacta atcgtgaaga
     8941 ttcttttttt agttaaaatc aatttagttt ttctttgata tcattgacca tttcctgact
     9001 ggcaataggt ccgttatata gccaactaga ttcaggtccg aattgcagta catgattgtc
     9061 tgtcactgct ttaagattct tccagcttgg ttcattgaac atggcagcat ctttgtcact
     9121 gttcacaagg atgatgtagt cagcatctaa ttcagctagc ttttcagagg aaacagcaga
     9181 ccagtcagaa gtggcttttt tggagacctc ttccactaga tcaggaacac caaattccag
     9241 atcgtcgtat acgattcttc cacttgaccg atttttgctg accataaagg ctgagttgtt
     9301 ggtcacccac aacactgcag ctgtcttgtt ctcgatttta tctgctagtt cttctcgagt
     9361 tttcttgatg gatttttgat aatcggaaat gattttttct gcctcttttt cttttgccaa
     9421 agctttgccg acatctttta gttgttcttc ccatgtgaca tctgttccgt tttttacaac
     9481 ataagttggc gcgattttgc tatattgttc gtatttcccg ccttcgacag tagcggaaga
     9541 agaaatcaac agcaaatcag gctcaaagct taacactttc tcataaggaa ggtcataaga
     9601 aatcgttggg acatccttta aatcatcttg cagataatct tgaatggaac cactgccaac
     9661 agtccactgg gcgataggtt tttcccctag tgcaatcaga tagtcttcta agtaactgcc
     9721 gatgatccgt ttgggcgcgt ttggaatctc tactttatgt ccaagtgtat ctgtgactgt
     9781 atgagtacta ttggatgaag tcgtcgaatt ggcagttgta gctgtttctt tatctgaaga
     9841 gggggagcaa gcgctcagta ctccagccag cagcaatatg ctagataatg tgaatacttt
     9901 tgtttttaat ttcatttctt tcctcctaac ataattgaga atctttctca ttggttatta
     9961 ttcctgatat ttcgaacgct gtcaaccgct tcatttggct atagatatga aagtagcgct
    10021 tgacgaaatg ttgaaacagc gtcataattg agaatgattt tcagcgaaag gatgggtgaa
    10081 atgacgagat taagagcagc tgacattgca gccggctacg aaaacaaaaa aatcattgaa
    10141 aacatgtctg ttacgattcc tgaagggaaa atcactagtt tgattgggcc aaatggaagt
    10201 ggaaaatcta ctttattgaa aagttttacc cgtatactac ctgtgaaatc aggaaagatc
    10261 attgtcaata actctgatat ctctacatac agttcaaaag aacttgcaaa aaaaattaca
    10321 ttattagcac aaactagtga acaccctaca ggtatgacag tagaagaagt agtatcttac
    10381 ggacgatacc cttatcaaaa actgttcagt ggcatggaca cgcaagatca tgaagcaatc
    10441 gagtgggcgt tggatgctac aggtctagct ggatttcgta tgcgggatat cgcaacgctg
    10501 tctggtggac aaacccagcg tgtctggatc gctatggcgc ttgctcagga aacagatatc
    10561 ttgatcttgg atgaaccgac aacattttta gatccggcac accaattaga gatcctacag
    10621 ttattgactg aaatcaatca aatgaaacaa acaacgatct tgatgtcgat tcatgacatt
    10681 aatcatgctt cacgtttttc agattattta ttagcaatga aaaacggtaa gttgatcaca
    10741 gaaggtacac ctgaagaagt gatcacaaaa gagtgtatga aaaaaatatt tgagattgat
    10801 gcaacgattg tcacgcttcc aagaacaaat aaaccagttt ttctaaccta tgatttgttg
    10861 ccaaaagagc aggaggatca attatgagga ctatgaagaa aaatagactc ctgcttttgg
    10921 tctgttttgt tcttttgatt attttttttg ttgctctttc aaatggcgca gcgactgtgg
    10981 gaaaagcaga tttttggcag gcgttgtttg attttgatag atcaaaacaa agtcaccaaa
    11041 tcatacgaaa tattcgtttg cctcgtgtgg cgggagcatt tcttgtaggg agttgttttg
    11101 cattgagcgg tgcattgatg caaggagtga caagaaatcc tttagcagat tcaggcttac
    11161 taggaatcaa cgcgggtgct tcgttggcaa tggcccttag ttttgctttt tggcctaaag
    11221 cttctgattt ttctgtgttt ctaatgtcat tggcaggttc ttttgctgta actttgctcg
    11281 tatttggttt tttgcagttt tcgaaaatgg gcatgaatcc tttacagctg attttagctg
    11341 gcgtagctat tagttctttt tttactgcta tcagccaagc acttacccaa ttctttaatc
    11401 tgcagcagga tcttgctttt tggtttgtag gcggagctgc caatatcaca tggaaacaat
    11461 tagcagtatt gtttccttta tatatacttg ctgttttggt ttctttttca ttaggaaaag
    11521 caatatcttt gattcatttg agtgaaacac atgcattagc tcttggaagt catcctaaac
    11581 gaacgcggtt gattgttttc tgtctagttg cagtgttagc agccattgcc gtttcgttag
    11641 tcggacctgt ttcatttatt gggctgttgg ttccgccagt tgttcgcgga atggttggaa
    11701 cggactatcg ttatgttcta cctgtctctt ttttagttgg cggtggattg gttatgttgg
    11761 cggattttac agcgaggatg atcaatcccc cttttgaaac accttttgga ttagtcattg
    11821 cattgattgg cgttccgttt ttactgatac aagtaaggag ggaacaacgg tgaaaaaaat
    11881 atattggacg ttctatttct tgctttttgt acttgtcatc ctgtctatag gtagtttaat
    11941 gattggtacg ccttttattt acctggatca gctttttgcc acgttcatag ggaaagaatc
    12001 atcttctttg gcgctgattg ttagagaatt tcgagttcct cgattactga tcagtttatt
    12061 agcaggagct tgtcttggcg tttctggtta tatcttgcaa ggagtaacaa gaaatgaatt
    12121 ggcagattct agtatattgg gcattaatgc cggagcagga tttttagtga tgctttattt
    12181 aggtttcttt tcgcactatt cgctgccgtt tctattgcta gttgtagcct ttctaggtgg
    12241 tgttttagct gcttgctgtg tctatcttgc ggcctatgat cgaaatggct ttttgggtat
    12301 gaatcgaatc ttattgtccg gtattgccgt taatgccggc ctttctgcat taacattgct
    12361 atgcacgatc caattatcta aggaaaatta tggatttgtg aatgcatggt tagcgggatc
    12421 aatctggggt gcgagctggc cttatgtttg ggcattgctt ccatgggcgg ttatcttaat
    12481 tccgctagga ggactttatg cacagaggat tggattgcta tcatttggtc aagaacgcgc
    12541 gcaaggatta ggccttaacg tggctaaaag tcaaaaaatc cttttgttgt tagctgttgc
    12601 tttggcaagc ggtagtgtag cggtgacagg tagtttatct tttgtaggac tactagctcc
    12661 tcatgctgcg aagctagtcg tacgtaaaga aaataactgg acctttgttc tgagcggttt
    12721 gttcggttcc atttttctgc ttagtgcaga tatggttgct agagtgattc taccaagtgg
    12781 ggaagtacct acaggaattt tgattgccct ttttggtgcg ccatattttc tgtatgcact
    12841 ttttatcaga caaaaacacg cgctgaacag ttgaaattta ttaaaaaagg aaggccaatc
    12901 atttctaggt cttcttttta ttctatttat gcatttattt gaaagttcga ttgctatttt
    12961 tctttttttc ctataccatt ttatgagaga aggaagggtg agtttttgga ccaattgaaa
    13021 gtaagattac gccggaaagg aatcgaaaag ctgaaaaatc tggccaaaag tggcaagaaa
    13081 aaggaaaaag aacagcgaat ctataaacag ctattcgcca gcgagtactg gaagtcagca
    13141 aaagtgatag ggacgacttt atcgaatgaa tttgaactgg atacacagcc ggttatccaa
    13201 cgagcagaaa aagaacaaaa gaaaatagtt gtacctaaaa ctttgccgaa acgtcagatg
    13261 gcgttttatg aaatggatga acagtccgtt cttgtacgtt cgagttttgg tgttcttgag
    13321 ccacaatcag agaagctcta tttgccggag atgatcgatc tcctcattgt accaggtatt
    13381 atctacaata gagccggtta tcgaatcggt tttggcggtg gttattacga ccggtattta
    13441 aataattttc ataaaaaaac atgtagcctt gtttttcgtg aacaattgaa tgaacaatgg
    13501 caaccggaga gctttgatca gaagatccaa caattgttta ttgactaaaa tgaaatggga
    13561 ggttttcatg aattatcaac agcaaataaa attgcgtgca tggttaagac gaccgttatg
    13621 gacctatgct tttttaggga tacagacagc ggtcttcctc atgatggaat tatttccgcg
    13681 acttgagatc ccttattatg ccggaatgta tggtccttat cttgttcact tcaatgaatg
    13741 gtggcgttta gtgacaccaa tcttcatcca ttttggactg atgcatttcg taatgaactc
    13801 actgatactg tattttatgg ggcagcaaat cgaggcaatc tatggccatt ggcgtttttt
    13861 cttgatttat atgttcagcg ggataatggg aaatacagca agctttgctt ttaacgaagc
    13921 gaatgttctg tctggtggtg cgagtacttc gatattcggt ttgttcggtg cgttgtttat
    13981 cttagggttt cattttaaac ataacccagc gatccagcag ctagtcagac attttctttt
    14041 atttattgtg ttgacatttg tttttggtct tctggatacc tctgtagatg tttggggaca
    14101 tgtcggtggg ctaattggcg gattagtgtt agggaatgtt ttgggtctac cgaaacaaca
    14161 gacaagctat tcgatacacc aaaggattct ttctacacta gtttttgtat ttctttttgt
    14221 aatatgtatt ttactgggtt taaaaaaata tggtttactt gtataatatg aaggaagtgc
    14281 ttaaagatgg agagcttata tgatgtacag cagctgttca aaaaatttgg catctttatc
    14341 tatgtgggtg cacgcatcta tgacattgaa ttaatgacta tcgaattgcg taatctgtat
    14401 gatagccgtt tgattgatca tgatacatac atgacagcgt ggcgcatatt aaaaagagag
    14461 caccgaattg aagaaagccg aaagaaagga aatttgtaat ggacaagaag attattggga
    14521 ttgatttagg tggaacaaca gcaaaatttg caattttaac accagaagga gagattcaac
    14581 aaaaatggag catcgatacc aatattttgg atgatggtaa gcatatcgtt cctgaaatca
    14641 ttgaatcaat caaccatcgt ttgaatttat ataatatgaa agccgaagac tttatcggaa
    14701 tcggcatggg taccccagga agcgtggaca gcgaagctgg aacggttatt ggtgcgtata
    14761 acttaaactg gacagaagtt caatttgtga aaaaactgat cgaggcagga acaggaatca
    14821 aattcgctat cgataatgat gcgaatgttg ctgctttagg tgaacgatgg aaaggtgctg
    14881 gagaaaacga tccagacgtt gtattcgtta ctctaggaac aggtgttggc ggtggtatcg
    14941 ttgctggcgg aaatttgatt cacggcgtga gcggagcagg cggagagatc ggccatatca
    15001 ctgtggatcc tgaagggttc gaatgtactt gcggtaaacg cggatgttta gaaactgtgt
    15061 ctagcgctac tggtgtggta cgtctaggtc gttacttggc agaagaatac gcaggagatt
    15121 caaaactgaa agcaatgtta gacaacggtg aagaagtaac aagtaaagat atctttgaaa
    15181 tggctcaaga agacgatccg tttgcattga tggttgttga tcgcgtatgc ttctacttag
    15241 gtttagcttg cgccaacttg gggaatacat tgaatccgtc aagtattgtg ttaggtggag
    15301 gagtatcggc agcaggagaa tttttacgta gccgtgtaga gaaatatttt aaagaattta
    15361 ctttcccgca agtaagagaa tcaacaaaaa tcaaattagc agagcttggt aatgaagctg
    15421 gtgtcattgg tgctgcctca ttagccttga aatttgctta aaaaaacgga ggcattacat
    15481 ggttttatgg gtaattaata tcattttgct attgattgtg cttgcaatca tcttttggga
    15541 agtctatcta cgagtaatgg ccaagcgttc agcaacgact ttgacagaag aagaatttcg
    15601 agaaggaatg agaaaagcac aagtaatcga tgttcgcgaa aaagatgtgt ttgatgctgg
    15661 gcatatttta ggtgcaagaa acatccctta cactgttttg aaagattcat taggatctat
    15721 tcgaaaagac cagcctgtct atatttacga tcaaaagaaa agcttgagca tccgtacagc
    15781 aaataaatta cgtaaagcag gttatcaaga gatttattta cttaagggtg gatatgacgg
    15841 atggtctgga aaaatcaaaa gtaaaaaagt ataaaacaga tgattgtatg acagctttaa
    15901 aaaccgaatc aatcattaga ttttgaccaa gatcacatga acggcgcaat ggatagttgt
    15961 gccgttcatt ttttttattt tgtgctatta tctacatata taagctaagc gaaaaggcag
    16021 aaagaaggaa acaactatga tcaaattaaa
//
LOCUS       DASHFF010000056        15931 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.056, whole genome shotgun sequence.
ACCESSION   DASHFF010000056 DASHFF010000000
VERSION     DASHFF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.056"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            133..3660
                     /locus_tag="U2I75_002068"
     CDS             133..3660
                     /locus_tag="U2I75_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN1697170.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="U2I75_002069"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="U2I75_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN1697171.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="U2I75_002070"
     CDS             7448..7789
                     /locus_tag="U2I75_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1697172.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="U2I75_002071"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="U2I75_002071"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN1697173.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="U2I75_002072"
     CDS             9146..11566
                     /locus_tag="U2I75_002072"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN1697174.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U2I75_002073"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U2I75_002073"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN1697175.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="U2I75_002074"
     CDS             13000..13737
                     /locus_tag="U2I75_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1697176.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="U2I75_002075"
     CDS             13751..14566
                     /locus_tag="U2I75_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1697177.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="U2I75_002076"
     CDS             14579..15226
                     /locus_tag="U2I75_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1697178.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSTLAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="U2I75_002077"
     CDS             15242..15898
                     /locus_tag="U2I75_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1697179.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
BASE COUNT         5341 a         2745 c         3376 g         4469 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcacctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta a
//
LOCUS       DASHFF010000057        15705 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.057, whole genome shotgun sequence.
ACCESSION   DASHFF010000057 DASHFF010000000
VERSION     DASHFF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.057"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1065)
                     /locus_tag="U2I75_002078"
     CDS             complement(<1..1065)
                     /locus_tag="U2I75_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1697180.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKI"
     gene            complement(1076..1744)
                     /locus_tag="U2I75_002079"
     CDS             complement(1076..1744)
                     /locus_tag="U2I75_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1697181.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1875..2459)
                     /locus_tag="U2I75_002080"
     CDS             complement(1875..2459)
                     /locus_tag="U2I75_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1697182.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2790..3023)
                     /locus_tag="U2I75_002081"
     CDS             complement(2790..3023)
                     /locus_tag="U2I75_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN1697183.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3038..3985)
                     /locus_tag="U2I75_002082"
     CDS             complement(3038..3985)
                     /locus_tag="U2I75_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1697184.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3985..4827)
                     /locus_tag="U2I75_002083"
     CDS             complement(3985..4827)
                     /locus_tag="U2I75_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1697185.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            5149..6336
                     /locus_tag="U2I75_002084"
     CDS             5149..6336
                     /locus_tag="U2I75_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1697186.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            complement(6428..7168)
                     /locus_tag="U2I75_002085"
     CDS             complement(6428..7168)
                     /locus_tag="U2I75_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1697187.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            7856..8014
                     /locus_tag="U2I75_002086"
     CDS             7856..8014
                     /locus_tag="U2I75_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697188.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            8086..9312
                     /locus_tag="U2I75_002087"
     CDS             8086..9312
                     /locus_tag="U2I75_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1697189.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(9472..10089)
                     /locus_tag="U2I75_002088"
     CDS             complement(9472..10089)
                     /locus_tag="U2I75_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1697190.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            complement(10772..11767)
                     /locus_tag="U2I75_002089"
     CDS             complement(10772..11767)
                     /locus_tag="U2I75_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1697191.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            complement(11862..12734)
                     /locus_tag="U2I75_002090"
     CDS             complement(11862..12734)
                     /locus_tag="U2I75_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1697192.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            complement(12747..14174)
                     /locus_tag="U2I75_002091"
     CDS             complement(12747..14174)
                     /locus_tag="U2I75_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEN1697193.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            complement(14228..15502)
                     /locus_tag="U2I75_002092"
     CDS             complement(14228..15502)
                     /locus_tag="U2I75_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1697194.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLFVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
BASE COUNT         5211 a         2873 c         2238 g         5383 t
ORIGIN      
        1 aattttttct tgctgatctt tactaactat ttcattcccc agggctaact cgctcctcac
       61 tgattctgtg aaaaattggt aatccatttc ttgcattact aaaaacggcg atgacaaatt
      121 ttttttattt tcgattatct ttcctttggt agggcgaata atcttcatga gtattcttag
      181 taaggttgtt ttccccacac cgtttttccc taccaaacca ataacatctc caactttgac
      241 atcaaaattt atgtctttta gaatattttc ataagtaatt tcttcaactt ttaatacatc
      301 tgcacttacc ttttcgatat agggaacttc aatcgaacta ttaaaaatgt caaatttcct
      361 taatgcgtta ttgttttcag tttttaacaa ctgaatagtt gtagttttta cagttctatt
      421 tttaacaaat agaacttgat caattaattc tttcaaatag taaaaacgat gttcagctat
      481 aacaatagta aatcctttat tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc
      541 tttgaagtcc aaatttgaag acggttcatc caatataatt aatttagagt ttagcgataa
      601 actacatcca atagaaattt tctgccgttc accacttgat agcgaaaaaa tgtttttatc
      661 taatatattt gaaagatcta ataattcagc taattcattg attcttttat ccatatgctc
      721 ttttgtaaat ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa
      781 gaattgtcct ctaggatttt gacttacgta gccaatattt tttgatatac tttcttgtgt
      841 taaggattgt agcgagttat tttcaaataa aatttcccca accaattttc cgtcataaag
      901 tcttggaata agtccattca ttaactttaa tagtgtggat ttcccactcc cagaagaacc
      961 agtaatcaat gtaagactgc ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc
     1021 cttaacatcc ttatatgaaa aagaaacatt gttaaattca aacaattctt tcacctcaaa
     1081 tcaaaatact taagaaaaaa atagtaacgg aaaacaggat gatatagtct aataagctca
     1141 tgtttgtttt atagtaagaa cttcttttca caggagattc tatccccttt gttattgcat
     1201 tagcggtcat ttcttcagca atcatagttg cacgtataag aacgggaact aacagaaatt
     1261 ccaaatattg cagaggctga aaaaaagaca agcgaatgtt tctcattttt aaagattctt
     1321 taatcattct tatttcagaa cgataagtcg gaaagaatct gatcactact gtaatactta
     1381 acattatttt tttcggtaaa tgtatttttt ctaaagagga taataattca ttgctagtat
     1441 agacactaac caacaaccat gagatcatta cgaccggcaa cattcgaaat acaatatata
     1501 aggatgtccc aaaaaatata gtaaaaaact ctatcttgat agcatcaata attttcatta
     1561 ctaaaaacag aactgaataa atcaagatga aataaataca tgtctttatt ttacgaaaca
     1621 tcaatactaa aactgtagca ataatgaaac accccacttg aaatatattt ccctttccta
     1681 aaaaaataat gaccggaaaa aaaatattta aagtaatcaa ggttaacgga ttgatttttg
     1741 acactctttc acctccaaaa aataagatcg cactgttatc ttaagctgac aaaaatgcac
     1801 cgttattcag ataacagtgc gatcttatca aaaagcataa ctgagaacaa ttctcaatgc
     1861 aagctttttt ttaattaact cagtaattca atccacccag gaacagaaaa ttgatacaat
     1921 cttctggtta atagcatagt cttttctttt gaatagctat gcttcaatat atcatagaat
     1981 gtagcgtaga atgagtaaat gtagaagtgg atttcttctt cattaaagac taaattttga
     2041 ttgatcattc cgatagtctt taacttatct agacaacaga ctgtcttttt tgtcacaaat
     2101 gatgtaattt tttcaacaat atcttgatat ttggttccag ctgattggaa taaaaccaaa
     2161 tcgaatatac gtttattctc aaaaatataa ttaataaatg gatctaattc attgaagttc
     2221 tctctccaac ttttctctat atctatattt tctaagttta agaggtattt ggtaaactct
     2281 tcattataca tattaataag cgcattaata tgcggctgaa taatttcttc aaataacgct
     2341 tccttagaag taaaatgttt ataaaaagca cccgtagtta cgcctgcggt caagcacagt
     2401 tgccttatct tagtttgctg aaatccttct ttttcaaaat ggcttagtcc actttctatt
     2461 aatttttggt gagttgtaat ataatctctg gtttctatgg gtacttcctc ctaataagat
     2521 gaattaacta tattatctta cacaggactt gaagtttcaa tcgttaacgt ctattactga
     2581 actcattcct tagagctaaa ctaatgagaa agagcaaaat aactgataca aaaagcaatc
     2641 ctacaagaat taatagactc atctaaatca ttcacctttt ctaaaataat ccgagaaaga
     2701 tttaaaaaaa ttatatccag ctattagggt aagtcctatc ataatcactc caatagccat
     2761 tttcttatcc tccctaaaaa taaagtcttt tacttaaaac cttttttgta atcgtcaata
     2821 aattttttcc ctttatcagt taattgaaat cctatatcta aatagtcctc atatctaagt
     2881 ttagccagca tctcacttac agatgctgga ctaacactta gaacttttgc taaatcatta
     2941 ttttttaatt tcttttttct ttttgataat tcctgaattg ctagtaaata aattagtata
     3001 gtagtcgttt taatttgtac catacctacc ctccaaatta tttcgaaagg ccttcagcaa
     3061 ttttatccag gttccatttc atcatcgcat agtaactatc accatcttgt cctttttctg
     3121 caattgaatc agtaaaaatc gttgaataga taggaatatt ggtatctttt gatactgttt
     3181 tcattggtct atcgtccaca ctgctttcta cgaataagga gggaactttt gttgtgcgta
     3241 atttttcaac taagtgtttt atttgatctg gtgttccttc ttcttcagtg ttgatttccc
     3301 aaatgtaagc agaaggcaca ttatacgctt tcgagaaata tttaaagcat ccttcacttg
     3361 taacaatcat ttttttatca ttcggaattg aagcaaattt agatttagct tctttgtcta
     3421 gagaatccaa tttttcaata tacttatcta gattttcttt atagaatttt ttattatcag
     3481 gatctttttc cgctaactgt ttttcaatat ttttagcgta aataataccg ttttctaaat
     3541 ttaaccaagc atggggatct tccttccctt tctcactctg accctctaag taaataacat
     3601 ctatgccatc acttgctgca aaatagtctt tgttttcctc tttgttcgca ttttttacta
     3661 attttgtaaa ccaagcattt cctccagttt ccaagttaac accgttataa aaaatcaaat
     3721 ctgcttttga agttttttga acatcttcag gcaaaggttc atattcgtgg ggatctttcc
     3781 caataggtac gatactgtgt aaatcaattt tatcttttgc tatattttca gtaatatctg
     3841 ctaaaatcga attagtaact actaccttta atttttcgtt cgaagccact gtatctttgt
     3901 ctttactatt tgttgaacat gccccgaata taaaaagagc aatcaaaatc cccagacttc
     3961 ccagtactgc tttccatttt ctcatcataa ctttctctcc ttctttctta aaaatttttg
     4021 ttttggtgag attagaaaac taattacaaa taagaatgcc gctgttaaaa caatacttga
     4081 tcctgctgct atgttaaaac tataaccaat gaacaaacct agcaatgatg agcacgctcc
     4141 gactgtcgcc gaaataaata tcatagtttt taagctgttt gcatataaat atgctgtagc
     4201 agctggtgta attaacattg caacaattaa tatagttccc acactttgca tagctgttac
     4261 tgctactaaa gttaatagaa tcattaatag ataatgataa aaactgacat tcatccccat
     4321 tgcttttgcc attaatggat caaaagaagt gattaataat tctttaaaaa atagaacaac
     4381 tgtaaccaac actaaaacag aaacgccaat tgtaatccac ttatcaatat cttgtacagc
     4441 taaaatattt ccaaataaga tatgaaataa atccgttgag ctatttgcaa ctcctataag
     4501 gataactcct aatgctaaaa aagaactgaa agtaatacca attgctgtat ctccttttat
     4561 tacactattg ctttttataa aagtaataat gatagaagct aatagaccaa atacaatcgc
     4621 accaataaaa aaattaatac ctaaaatata agaaattgct actcctggca aaacagcatg
     4681 agaaatagca tcacccataa gagacattcc tcttaaaata ataaaacaac ctactgttcc
     4741 agaaacaatc cctataacta cagatgtaat tagagcattc tgtaaaaagt gaaagtcata
     4801 cagaccatta ataaaatttt gaagcatatt attccccctt gtatattaga gttagggttt
     4861 taaccttccg gttttgattg gataacccta tgaaccatcg ttattttaat ttggtattaa
     4921 ctcatttctt ttgagataat atatttattg gattgagagg ctaagccgtt ccccccttgg
     4981 gccattgaac ccgcactcga gagactggct cctctctctg ataagtggat tgcgtatgag
     5041 ttagatgttg aaagtcctta ctttactccg tatctcgtac aaggtagtga cgcttttcag
     5101 ttgagtggat ttcaatccaa aattcttttt ttgaaaggtc agatatacat gtacttttta
     5161 ggcattgata tcggtaaacg cactcacgtt gcttctatca tgaacgagga aggaaaggta
     5221 ttactaaaag gtttttcatt tcctaacacc actgaaggag cagagtcatt aattgagcga
     5281 atggtcgact attccggagc acccagtgac tttgttatag ggatggaagc cacggggcac
     5341 tattggctgt ccatcttttc ataccttcac gaatcagatt atttgattca cgttgtgaat
     5401 ccccttcaaa cagacggatg gcgtaaaggt acagaaatac ggaaacgaaa aaacgatatt
     5461 attgattctg ttttgattgc tgacttaatg cgctatggct catttgtcga aacggtctta
     5521 tccgatgaaa atgttttttc tttgaaacaa ctttctcgtt accgaactta tttagtaggt
     5581 acagcgagcg acttcaaaag aaaaattata gccgtattgg atcaagtctt tccagaatac
     5641 gcgacgattt ttaccaagca gggcgttttc ggaaaagctt ctaaagagtt attgctggcg
     5701 tttagtgccc cgatcgactt agaaaacatt tcctcagaaa ctttggccca atacttagct
     5761 gagttaagca ggaaccgaat caaggcagaa aaagcggacc aattgaagca agcagcttct
     5821 aactcctttg gagtaaaatt tgctcaagaa gcgttcacct tccaactacg ttcaatgatt
     5881 gaacaattaa aattcattga aagccaaata aaggaaacgg aaagagaaat caagcacata
     5941 atggacactt taaattctgt tattctcaca attcctggaa ttggtcctat aaacggtgcg
     6001 actattcttg gtgagatcgg agatattcaa aaattttcta acccgaaaaa actcgttgct
     6061 tacgctggga tagacgcttc cgtgacacaa tcagggcaat atgaggcaac gcataatgtt
     6121 atgagtaaac gagggtctcc atatttgcga aaagccttgt tttcggcggc tttagtagct
     6181 tctcaatgtg atcctgtttt tagtgcgttt taccagaaaa aagtttccga aggaaaacat
     6241 catctaactg ccttaggtgc ggtttctcgt aagctttgtt acgtgattca tgctattttg
     6301 actaaaaacg agccttatat aattgtttct gattagctat tgaacattca aaaaaactaa
     6361 tggtaagaag cttatttgtt gtaccctttt ttcggaatta tctgttataa aatatttttt
     6421 gatttggcta atttttactt gactctatat agttagtctc tccaataaat atagtatctc
     6481 cataggcttt ttttaaattc tcttcattaa atacagattc tacacttccg tgagcaatta
     6541 gatttcgatt caaaagaata atattatcaa aatacttctt taccttgctt aaatcatgat
     6601 gcacaattag cactgttttt ccctgatgtt tcaattcttt taacgtatcc ataattattc
     6661 gttcactaac taagtctatc cctacaaatg gttcatctaa aaatatgaaa tccgcatttt
     6721 gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc tccagataat tctcctattt
     6781 gatgattaga atattttccc atatcgacct tttccaaagc ctttgaaact ctttgccatt
     6841 ctgcattttt aattctttga aaaactttca ttccggcata tgttcccata gatacacatt
     6901 cttttacttt tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct
     6961 tcaccagttt tttattgata tcttcattat taatggagac ctctccctga tgagggataa
     7021 tatttaacat tgctttaagc aatgtagatt ttccagcacc atttggacca ataatacctg
     7081 ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac
     7141 aaacattaag ctttttcact tctaacataa ctttctcctt ctaaatgata atgattatca
     7201 cgatcatatg gtatacaaaa attatatttt agtcaaccct aaattttata atttagattt
     7261 ttttctgttg ctgaagatta gaatgacaaa actatttgaa actacgcgta tagtaagtag
     7321 ctttcaaaat atataaaaaa ggagcgttct caaatgagaa catgctccta aaactacttt
     7381 attggcgtat ggaaaaatta tacttaaagt ggatagcggg ttgaataagt tgataagcat
     7441 caaaacattt ataaaaatta ttcaagacta tgtctgttcc atatacaaat atgaaaatca
     7501 gtaaactcca tttaactttt ttcgtagtgt gtctttttat acatgtttat cgttattaat
     7561 agtgaatagg aaaaaaccta tttttgatca ttgacaatta aatacaattt ttgaataacg
     7621 atccctgcta ttcgagctag aaactcaagt acgccatgac ctgattagcg acaaatactt
     7681 agagatttct ttttgttctg ggaaaacaaa atgccatgac aatagcagca aataatgata
     7741 cttccgtaag gctcgccgta aactggggag aggtgaaatt cctatgtgct tacccaaagc
     7801 aattcaaaaa ttctaatctt ttttaggaga tgattttata aaattgagag tctacttgaa
     7861 taaaaaacaa acctgtgagt ttctaggaat tgataataat actttagata agtggattcg
     7921 tatatataat tttccagtaa tcagaattga cggtatagtt cgctttgaca tagaggatat
     7981 caaagatttt atgaatacat ttaaaacaat ttgaaaattt cttctcgccg catatactaa
     8041 gaatagcgaa atatactgcc ttacttttta agggagtgag gaattttgcc tacaaaactt
     8101 tcaaatggga aatataaaac taatctgagg tatccaaaaa aattcagaga aatcactgga
     8161 attacatcag aaaagtatca aaaagtattt ccaactaggc aattggctat taaagccgaa
     8221 aatgcaatga aaaggaaaat tgaaacagtt ttaagagagg agaatgcaaa ttcactagaa
     8281 ttaaaaggaa aaataaaatt taaggaattc tatgaatcaa aatggcttcc tagatatgaa
     8341 ctaggtcaga ctactagatc aaaaagagct ccaagttacg taacaattag taatactaaa
     8401 gatatattcc gattgcacat ccttccaatg tttggtgagt atgcaatgaa ctatctaaat
     8461 tcaaatactg agataatatc agatgaatta acaaaaaaat cgaaagaata cgcaaatata
     8521 aaaattatca aaggatatgt ccgttctatg tttgatattg cagaaatact aaattatatt
     8581 gaatttaacc gaactaccaa aattattcaa tgtatcactg cacctaaaaa aattgcttta
     8641 gaagaaagaa ggatacggga aggtaatcaa gcattatcgt cagaagagct tattgcttgg
     8701 atagaagcag taaacactga cttgaataat cacttgctta ctcttcatga ctatactctt
     8761 ttcatgctta ccctatatct tggtgataga aaaagtgaga cgtatgcttt gcaatggaaa
     8821 tatatcgact ttgagaaaca aacggttcgc cttaaacatg ctttggataa atatcaaaaa
     8881 aagaaattta caaaaggtcg aaaggatacc gtaattcaag tacccgaaat tgtaatggca
     8941 ttactaagca aatggaaatc agtgcaagct gagcaattgt tgagattaaa aattaatcaa
     9001 acaccagatc agtatctctt tacctatact aaaccatccg gagaagtaaa ttgccctgtt
     9061 cacgctgatt acctaaacta tcgaatcaat tcaattaaaa gacgacatcc tgaattggct
     9121 catttatctc cacataagct tcgtcatacc tatgcaacta tagctagaca aggcggagca
     9181 aatatgaatc aaatatctag tgctttaaca cactcagata tttctacaac gagaatatac
     9241 gtaaatacac cagatgtagt agataaaact gtttttgaag cctttcagag aggattgaat
     9301 aaatgtgatt agtatgtgtt cttaaatgtg ttcttaaaca agaaaaagac acttttcaaa
     9361 caattgaaaa gtgccttgaa acattgatat ataaataata ttaacgttta gagaattgag
     9421 atgctttacg agcctttttg agacctggtt tcttgcaaaa ttatattagc ctcaactgaa
     9481 agatttattt ttttgctccc taacttcatt agtaatacca tgtttcattc gatatcttct
     9541 aaacgttcta cgattaatcc ctgttagctc ttcaacttct ttatctgtag atccagtcaa
     9601 aaacaaatta aatgctaatt gaagtcgcgg atcattctca cgatatttta gcgtacctcc
     9661 cttgtatttc ccttttgctt tagcgagtgc aattccttgt tgttgcgttt cgcgaatata
     9721 ccgacgttct tcttcggcct ggtacttgta tagttcgata attaagttat taagtaatcg
     9781 acgtaaatta tcatcagtga tacctgttaa cgctggcaaa ttcaaaactt ccagtgtagc
     9841 tttcttcagc tgaattaaat tcatcgtttc tgttagctct ttattatttc gtccaagtcg
     9901 ttttaattct gtcacaacga caatatcacc ttcacgtatg aatttcatca tttcttgaaa
     9961 tccttctcga ttggtatctt tcccacttgc tttatcacta aaaattttat cacactctga
    10021 taataattct aattgtcggt ctaaattttg atctaagctg cttactcgag catacccaat
    10081 tttcgccata ataaaactcc tttaaaattc tgaaatccgg atcattttta aattctgttg
    10141 cccaactctg ttgtcatttt tctagataaa tggcataaat tttcagcgct aagtgacaaa
    10201 agcaactaat ttttcatgat agatttttaa atatctcaat ctatctccaa aattagttgc
    10261 ttttgtagct atagttgact atgaatatga caaataatta gaaaaaactg tgccaattaa
    10321 atataaaaat gacactcatt tacattcaat agaacaccaa gagcccaaca gggtatactc
    10381 tggtgtcctt ttaaaaaagt aactttctga cctaattaat tgagctacta aaaaagctat
    10441 gaaatgagga ataaaaaagg gatcaaaaac ttttaagttt ctgacccttt tgagttctaa
    10501 ttatttcttc cagaacgaag aatgttatct gctttatctg caattgattc tgttagaagg
    10561 actgaaaaat ttatgccagc ccttttgcct agcttatccg cccattgagg aattgttaag
    10621 gtttttctga caagttctat gtctttcaaa tatttggcaa catctacaca gattaaaagc
    10681 atgattttgt atcatagtct attataagag atttaacaat tttttatatc acgaattaat
    10741 aatgaaatta tcattgatag tctttataaa tttaattgtt ctttttcttt actgatgtct
    10801 tccccatcca taatgtgaca ggtaaaatat attccttttt tgtttcttct ttatttattc
    10861 gtttcatcaa aatagcaaca gctgtgtctg ctatatcatt aattggttgt attactgttg
    10921 tcagatctgg taatatactc cgagtcatta aagtaccatc atatccgact actaataaat
    10981 cagctggtac atttaacccc ttttcttttg ctacttgtaa tattagggcc gcatcgatgt
    11041 catttgaagc aaaaatacca tctattttag gataatcctc aaacatttgg aaaaaaatct
    11101 gttttttctt tacataagaa atattgaaat ctaatgtcac agttcgtgga attaactgat
    11161 atgctttcat agtatcttca taggctagtc tccgacgatt agccggagtc tgtaagtcaa
    11221 ttggaccgtt tgtatgaata atattttttg caccttgtgc aattaataac ttggttgcaa
    11281 gttttccacc attgtagtta tcggattcaa caactggaat atcttcattc atcactcgat
    11341 caattgcaac aatcggtaaa ttctgatatt tatattcctg aattccttgg ttatgtgtac
    11401 caacaattaa accatctact tgatcagaca ataactgatt taaataattt gtttcctttt
    11461 ctttattatt catcgaattg cctattaata ccttataacc aatttcatat aacttttttt
    11521 ccaatgcctc aacgagttca ctaaaaaaag gattggctac tgtaggaaat agtaacccga
    11581 caatgttagt tttcttttga aataattgac gcgctacaac gttcggttga taattgagtt
    11641 cttctatagc cttataaaca ttatcaatcg ttttttgact taaatagcca cgattgttta
    11701 atacacgtga aacagttgtt tttgatacat ttgctctttt ggcaacatct tctaattttg
    11761 ggcgcattct cttgtctctc ctaaataatg caataattaa ctatcgaaaa aaagaactct
    11821 atagaactac tatacctgat aatcgatatt ttttctatgt tttattcttg taattgattc
    11881 aagcccaaag caaagtttcc tagtgttgcc gatccattat ttttaattaa aggacgcgta
    11941 atatatttac ttaacggtgg tatttccacg taatcatcca ataatttatt aacattaaca
    12001 cgaatttttt ctaacaattt ttcatttact acgcttccac caaatactat tttatccggt
    12061 cgtataatca atgttgtttg aatcacagct tgtgctagat aataggccat tacatcccaa
    12121 ataggatccg ttaaagaaac gtcttcaccc ttttttccaa aacgtgcttc aaacgttggt
    12181 cccgcaacta agccttctaa acaatcacca tgataaggac aaattccttt aaaatcaata
    12241 tcatcaggat ggcgttttac aaacgtatgg cccatttctg aatgaccaaa cgatcctaat
    12301 agttttccgt caacaacagc tccaccacca acgccagtac caacggtgta ataaactaat
    12361 gaatttattt tttcattcga taaagtggac atgatgtact ctccataagc tgatccgttc
    12421 acatccgttg tccaagacat tggaacattc agctcatctt gtaaataacc taacacatta
    12481 acatttcgcc atcccacctt tggtgtagac gtaatatatc ctgattttgg tgaatttttt
    12541 cgaatttcta ttggaccaaa tgatgcaata cctaatgcat ttatatcaaa ctgtttaaaa
    12601 tagtcaacag tttttttcaa tgtttccctg ggggtggtcg ttggacaaat tatttggtct
    12661 tgtactcggt aatcctcgtc tcccacggca catacaaatt tagtcccacc tgcttctatg
    12721 ctaccaatta acatcataaa acctccttac tcgtaagaaa ttgcatcttt ttttagatga
    12781 taaattgtac tcgagacaaa agcttcttca ctaacactca aatgcatact tggttgattt
    12841 tctgtataat aacgctctgt aaaaacaagc tcgccatcat taataaaaat ctctgccgaa
    12901 cttttatcga taaacactcg aagtgataat ctatcacaca tctgaatggt tccatacctt
    12961 ggatcatttt tgccgttacg agttaaggta cattcatttt tatccttaga ataaaaaata
    13021 ttgattaact cttcatcttt tgtttttaat gaaaatttga cttcttgatt ttttgattta
    13081 gtcaatagta catcaattag agcttctttg aatgaagcat cttgagcgat tattatttct
    13141 cctgccaaat taactgtcgt ttcaacgccg ctgccaactc gaagttgttc taattctctc
    13201 acaggtttca tatacaaacg atctccaatt aactctaatt ctcgaggaag cgttaatgct
    13261 cctgcccatc cgtctacttt ttcaggcata gggctttccc acatatccat ccaggccata
    13321 acaatacgtc taccatctgg agataaagtt gtttgtgttg catagaaatc gtgtccctta
    13381 tctaattcat aaaaatttcc tctaatgaat tttgctgtct cataatcata atcaccgata
    13441 aagtagcctg tttcatatag atttaaataa tcttttccat ttgcttcaat tccctgaggg
    13501 gataataaaa gaatagattt tccatttaat tcaaaaaagt ctgggcattc ccacataaat
    13561 ccttctgtaa gtacttcctt cgctttagaa attggtccaa tgtaatccca atttaataaa
    13621 tctaacgact gataaacaat agcacgacct aatccatctt tttcttgact acccaaaacc
    13681 atgtaatatt tttcatcgtg tttccatact tttggatctc tgaaatgatg tgtgttatct
    13741 tctggtgctt ttgctattac aggatttttt tcatatttag taaagtgaat gccatccgta
    13801 ctatacgcca tgttttgatt ttgccaaaaa tgatctggat cattgtctcc ataatagtga
    13861 tgtcctgtat agaacagata caacacacca tttttttcaa tagcgcttcc tgaaaaacaa
    13921 ccaccttcat cttcttgatc tccaggagtc aaagcaagtg gtaaagattc ccaatgaact
    13981 aaatccttac ttctagcatg tgcccagtgc attggtcccc attcagcact atatggatga
    14041 tgttgataaa acacatgata atacccatca aaatagcaaa atccatttgg atcattaatc
    14101 catcctgatg gtgcagacac atgatatccc aaacgataac gtgtatttgt taattctata
    14161 gctctttttg tcataatata ttcgctcact ttctagataa aattgttttt tattctgttg
    14221 gtttccatta tcctcgaata aatggatccc cttgaacatc tctatcatct tgtttaagaa
    14281 taaagaatgc atatatgcct gctagtaata caataagaga aataatttta aatgttggtt
    14341 gatagccaac gttgtctctt aatattccca atggtgttga taaaatcact tgcccaattt
    14401 gagcagctat ctgaaatcct atcatataga gggttgctga tagctttgta tcaaaatgaa
    14461 gtgtaaaata gcgaaacatt ggtaatgtaa ataatggtac ttctaaagca tgcaacattt
    14521 ttatgcatga aacaaataat ggtgtcgagg caaacccgca caatccaatt cgtacacaca
    14581 taatggttac acctaataac aaagtatttc gaacccctaa ttttctcata atgattggaa
    14641 caattcccat cattaacgct tcacaaaata cttgaatagc attcaatgtc ccatatattt
    14701 tttcaccatt agctgatgtt gaaaacaaac cagtataaaa tcccggaaac atttgttgat
    14761 cgaataccgt ataaaatgtc catgtaaaaa caataaagat gattatcgtc cataattgag
    14821 gcagtttcat tagatcaaac atttctttta aagaaggaat actatttgat tcagcttgtt
    14881 cttgattaaa attttgattt gcttcccgtt caactttagg gttccaaaat aataaattta
    14941 acaatagtaa agaaccaaag aaagatcccg cccaaaagtt taaatgagga ttttttacaa
    15001 ataaaaatcc tgccaaaagc gctaccaagg cataaccaaa tgatccccac gcccttgctt
    15061 gtccatattc aaagccaaat aaacgactaa acctttctga cacagcttca aaaactccta
    15121 cagaagctaa atatccagct gacaaaaata gcgctcccat aataatgcct aaattaaaat
    15181 tgttatctag caatggccca tatatccata taaaaaatgg tccaacaagt gtcgctaata
    15241 cagaagcaaa aattaataaa tttcgtttaa tatacaattt atcttgtaat gttccataaa
    15301 taaacatcaa aattaaggta actaacgaat ttgccgagaa tactgttcct acagcactgc
    15361 cagataaccc taaaccattt gattcagatg ttagccaaag ttgaaaaaat gaccaccaaa
    15421 caccccaaga agcaaaaaat agtaataatg tagctgaact ttgtaaataa gattgatttt
    15481 taaatacgtt aacttttgac atatttttat cctccaacga tatatttgta tcccctttca
    15541 tgataaccgg ttaacatttt tagaaaagga cttgtactcc taaaattata aaagaaatcc
    15601 gcttacatgt caaccggtta ttctatttgg ataatgccat ataatgtcac aggatatgac
    15661 ttttattggt gtgtcgcttt tcttttttaa tggcactatt tttta
//
LOCUS       DASHFF010000058        15413 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.058, whole genome shotgun sequence.
ACCESSION   DASHFF010000058 DASHFF010000000
VERSION     DASHFF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.058"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(41..964)
                     /locus_tag="U2I75_002093"
     CDS             complement(41..964)
                     /locus_tag="U2I75_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697195.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(1703..2989)
                     /locus_tag="U2I75_002094"
     CDS             complement(1703..2989)
                     /locus_tag="U2I75_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1697196.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(2982..4250)
                     /locus_tag="U2I75_002095"
     CDS             complement(2982..4250)
                     /locus_tag="U2I75_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1697197.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            4502..4963
                     /gene="mscL"
                     /locus_tag="U2I75_002096"
     CDS             4502..4963
                     /gene="mscL"
                     /locus_tag="U2I75_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN1697198.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(5022..5468)
                     /locus_tag="U2I75_002097"
     CDS             complement(5022..5468)
                     /locus_tag="U2I75_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1697199.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(5561..5806)
                     /locus_tag="U2I75_002098"
     CDS             complement(5561..5806)
                     /locus_tag="U2I75_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN1697200.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(5921..6499)
                     /locus_tag="U2I75_002099"
     CDS             complement(5921..6499)
                     /locus_tag="U2I75_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1697201.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(6576..7106)
                     /locus_tag="U2I75_002100"
     CDS             complement(6576..7106)
                     /locus_tag="U2I75_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN1697202.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(7110..7970)
                     /gene="rlmB"
                     /locus_tag="U2I75_002101"
     CDS             complement(7110..7970)
                     /gene="rlmB"
                     /locus_tag="U2I75_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN1697203.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(7963..8358)
                     /locus_tag="U2I75_002102"
     CDS             complement(7963..8358)
                     /locus_tag="U2I75_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN1697204.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(8355..9767)
                     /locus_tag="U2I75_002103"
     CDS             complement(8355..9767)
                     /locus_tag="U2I75_002103"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN1697205.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(9764..10300)
                     /gene="cysE"
                     /locus_tag="U2I75_002104"
     CDS             complement(9764..10300)
                     /gene="cysE"
                     /locus_tag="U2I75_002104"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN1697206.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(10386..10625)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10622..12082)
                     /locus_tag="U2I75_002105"
     CDS             complement(10622..12082)
                     /locus_tag="U2I75_002105"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN1697207.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(12252..13409)
                     /locus_tag="U2I75_002106"
     CDS             complement(12252..13409)
                     /locus_tag="U2I75_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN1697208.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(13443..14813)
                     /gene="radA"
                     /locus_tag="U2I75_002107"
     CDS             complement(13443..14813)
                     /gene="radA"
                     /locus_tag="U2I75_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN1697209.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(14876..15403)
                     /locus_tag="U2I75_002108"
     CDS             complement(14876..15403)
                     /locus_tag="U2I75_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN1697210.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         4037 a         3375 c         2774 g         5227 t
ORIGIN      
        1 atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag
       61 taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt acggattctc
      121 cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg
      181 tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg
      241 cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca agggttacag
      301 gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta
      361 ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg
      421 tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag
      481 aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga
      541 caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc
      601 gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct
      661 gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat
      721 ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt
      781 taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt
      841 ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt
      901 tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa
      961 gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca
     1021 gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata
     1081 tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc
     1141 aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa
     1201 aaccggcaaa atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg
     1261 ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc
     1321 caaccaagtt aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca
     1381 gggcagcttt ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata
     1441 agccgaaata tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
     1501 gctgaccact tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt
     1561 tgattaaaag agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga
     1621 ataccataaa agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga
     1681 cttttcctca taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt
     1741 cacttataaa tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt
     1801 caggaagatc aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat
     1861 attcgatcga atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga
     1921 gatttgtttc tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt
     1981 ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat
     2041 ccaagctgaa ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat
     2101 agtttcgaga agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc
     2161 gtaacagttc tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt
     2221 tatctcgtgt aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag
     2281 ggaaataacg tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa
     2341 taagtttcat caatttctca ggctccatct taccaactac aaataaaacc atattgcttg
     2401 gctgataaaa cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt
     2461 caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc
     2521 caaaaaacat ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc
     2581 ctttttcctt gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag
     2641 tcgtcagatt cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac
     2701 ttgtgaaagc attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt
     2761 ctttttcaaa caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt
     2821 ctttttcacc atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac
     2881 catatgtctt gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta
     2941 cttcatgata aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct
     3001 cctgctaaaa agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg
     3061 atcgttacgc tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc
     3121 cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat
     3181 tggtttctca gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc
     3241 aaactttcta attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata
     3301 ccggtttgga cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct
     3361 agactttctt tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa
     3421 ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa
     3481 gctaggttca gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc
     3541 acttgaggtt gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat
     3601 tgcccaactg cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt
     3661 tgatcatttg ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc
     3721 aaatcttcga ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt
     3781 tcttgcaagg cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga
     3841 cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt
     3901 ggataaaata aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg
     3961 acatattcac cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt
     4021 ctaactgata atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc
     4081 tgatccggat aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg
     4141 atgacttctt gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt
     4201 tttgttggta agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc
     4261 tccttttcac agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt
     4321 attcctctgc ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac
     4381 actttatagt atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat
     4441 tgttttttta ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt
     4501 catgatcaaa gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg
     4561 cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac
     4621 accactaata ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact
     4681 ttcaattctt gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag
     4741 tgctatcatt acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa
     4801 ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc
     4861 agaagattat cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac
     4921 acatccagta gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa
     4981 agaaagccga ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc
     5041 tttttcatgt cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat
     5101 ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct
     5161 ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct
     5221 ttacggttgt cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat
     5281 actcgcgtgt aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttgt
     5341 ccatcaatgt catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt
     5401 cgttctttga cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga
     5461 tattccacta agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa
     5521 aaagcaaacg aaccttacga atctcaggct cgtttgcttt ttaaactgct tcagttaaaa
     5581 attcgatctc aactgtccga gtaagtacat ctgaatagct gtaagatact cgttcaaaag
     5641 aattttcttc aggatccaaa tcaacaacga atactgaagg ataggtttct gttaaaacgc
     5701 cgcggcgttc tgtttgacgc tttcttcctg tctgagcaac caacatgatt gggctgccga
     5761 tacgaccttc taaatctttc ttgattgaag ctaaagttgt tggcatacag ttcacctcta
     5821 ctatcaacta taccacatat ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg
     5881 caataaaaat aaataagaag ctcattaaga tttttctaaa ttattcctca ttataaaatt
     5941 tcaagtgttt ttttattttg agttttactc tcccgtaagc gctcattact tgcttgtgat
     6001 ccattgctaa acattcagcc atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa
     6061 ttttagcttc tatttttgaa aactggattt cgcaattttc gattgcttct tttaataaaa
     6121 gatgctgaat cgctaaaggt tcttgcaatg tgatctctgc taaaaagtct ggtccttctg
     6181 tctctatttt ttggtcgatt gagacttcat ctttttgatc acgacgcttc agcgcgttct
     6241 gcttcctcaa cagagaatag atttgattgc ttataatgct tttataataa cttccaaagc
     6301 taacaccttt gcttttttcg taagttctca atgcctgata taaagcgatc cttgcttctt
     6361 gttcccaatc ctcagaaaca taatttctta aataataggt gtttttcaaa cgtagtaaaa
     6421 gtggaaaata tttttgatac agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc
     6481 gcaataactc ctcagacata aaataattcc tccataaatt cgtttatgga ggaatcttat
     6541 catcaatgat tcatcgaact gattagaacg atatgttatt tattttttct cattaaatcg
     6601 tccaattttt gggacaattt ttttaactgc tcttcattcc atggagaatt acgtcggaaa
     6661 tcaataaaat gcatatcttt attttcttgg gctattgttt tttccgtttt ttttatcgtc
     6721 ttatataatt cataagcaga agttcgtaat gctccttggg aaaagatcat ccactgttct
     6781 gctaaatcgc ttgtcgccac tgtcacttgt gtcaaacggt cattcagctg accagcgatt
     6841 ctctcgatat aactgtcagc cgtttcgtct tctttcgtaa aaacaacatc caactggtat
     6901 ttcttataat tttgttggat gcccggaact agttgggcat cgaagacaac gatgacttcc
     6961 attccttcat atttcgcata atttgataaa cgatgtaaaa gagcttctct tgcatccgcc
     7021 attttgtcct gctttttaag ctggacaagt tccggccacg aaccaatcat gttgtagccg
     7081 tctacaatca acagctgttt tttcatggtc tactccccct gacgttggga aaaagcctga
     7141 tacataagca aaccagcagc aacactggca tttaagctct ggacgtgccc ggtcatcggg
     7201 atcgtcagca attcatccat ttcttttttc aatcctggac tcattccttt gccttcatta
     7261 ccaatgacta aagcaattga tcctttggca ttccattttt ggaagtttgt gccggtcata
     7321 tctgtgccaa atacccaaaa tcctgtttct ttgagctgtt gcacagcttg taccaaattc
     7381 gtcacgcgtg caacagggac atgttcgact gccccagtgg aagtctttgt tacgataggt
     7441 gtgattccaa ctgctcggtg ttttggtatg atcacaccat cgacgccact tgcgtctgct
     7501 gtccgtaaaa tcgacccaaa attgtgtgga tcttcgatgc tatccaaaat caaaaagaag
     7561 gggtcttctt ttttctcttt tgttcgttca atcagttggt caagggataa atattcataa
     7621 ggtgtaatcg caagaacgac tccttgatgc acgccgtgat cgctcaaagt ttctaatttt
     7681 tgtttaggaa cccatttgac tggcacagct tgttcacgtg cgatttcttt cagttcttct
     7741 actttttctc ctcgtgaatc ttcttgaaga aagagtttat tccctcgccc ttgttgtagc
     7801 gcttctactg cggcatggtg cccaaagacg aaattatctt ctaatgcttc ttcattttga
     7861 tcagaaactg actgatttcg tccagtttgc tttttatttg aatatctatt ttgttttttg
     7921 tcttcaaatc cacgtttcga ttgatgtttg tttgttcttt ctttacccat ttttttcacc
     7981 aactttctga atacaccagc ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg
     8041 cagatagccc atgagcgctt cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt
     8101 ttttgcgctc gtatggcttt tagcatttct tcctctgcga taataaagtt cttcttcttc
     8161 tgtcaaaaga tgttcttcca gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac
     8221 ataattcgtt gccgcacgat gcagcttatt gggttttgtt tctccttgat caatcaggtg
     8281 gtcacgaaca tagatttcgt aaatcgcatc tccaacataa gccaaggcta aaccgttcaa
     8341 ttgtgtgtaa tctctcatgc ttctcttctc catcgggttc cttgcggtgt atcttctaat
     8401 aagatccctt tttctttcaa caaatcacga atttcgtcgc ttcggacaaa atcacgattg
     8461 cttcttgctt gatttcgttc ttcgatcaat tgttcaatct cttcatctaa taactcttct
     8521 gctgtaaata aaataccgaa gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc
     8581 aagacagcgg cagaaacttt cggctggtct agataagtgt tcagccactt agcaagctca
     8641 tagaccactg tgatcccatt tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc
     8701 tgttctaaat tttctaacaa agcgatttta tccgcatcat ctgccaaatt ctctgaggca
     8761 gtttcttggc ggaatttcaa attgtcaaac gtattcttca gtcgttgtaa attggtggct
     8821 gcttctttca tcgtcgtttc actgtagcgg atcggacgac ggtattgtgt ggtcgccatg
     8881 aagaaacgca agacttgtgg atcaacgttt ttgatcattt catgtacagt gatgaaattc
     8941 cctagagatt tactcatttt ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc
     9001 cagtaattag cgaatttttt ccctgttttc gcttcacttt gtgcgatttc attttcatga
     9061 tgaggaaatt ctaaatcttg cccgccacca tggatatcga tcgtttctcc taaatgtttt
     9121 gttgccataa cggaacattc aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc
     9181 caagaaatct cgccaggttt tgcttgtttc cataaagcga aatctaatgg gtcttctttt
     9241 tgctgctgtt cttcccctgt acgctgactt gctccgactt ccagttcatc gatggattgg
     9301 tcacttaatt tcccataatt ttcaaattta cgggtacggt aatacacatc acctgaagat
     9361 tcataagcat agcctttttc aaccaacact tgaataaatg ctaaaatatc tggcatatga
     9421 tccatgactc ttggatgcag tgtagcaggc tcgacattca acgctttcgt atcttcttca
     9481 aatgctcgga tgaaacgatc agcaacttct ggtgctgtaa tacctaattc gtttgctgta
     9541 cggatgattt tatcgtctac atccgtgaaa ttcgatacga aatcgacttt atagcctcga
     9601 tattcaaaat agcgtcgaat tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg
     9661 atataattat acacagtcgg tccgcagaca tacataccga catgtccctt ttgtataggg
     9721 atgaattctt ctttttttct ggtcaatgta ttgtagattt ttatcattct gatctcctca
     9781 ctttttctcc tttgattcga acgacttttg ctggtactcc taccgctgta gcgtcgtctg
     9841 gaacatcttt caacacaacg gcacctgcac cgattttggc gttttttccg atcgttaccg
     9901 gtcctaatat ctgcgcattc gcagagagca tggctccttg tttaacagtt gggtgtcttt
     9961 tacccgtttc ttttcctgtg ccgcctaatg ttacgccatg gaataaaatc acatcgtcct
    10021 cgatttctgc ggtttcgcca atgacgattc ccattccatg gtcgatgaac acgccagttc
    10081 caatcgttgc tcctggatgg atctcaacac cagttaggaa acgccagaat tgagcattga
    10141 tcttcgccaa taaaaagagc cggtgctgat ataaaaaatg agagaaacga tgccaaaaca
    10201 aggcatgaaa accagggtat gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt
    10261 ttctctttgc tgcatcaatc gctcgggtaa accaactcat gactttccac tcctttcgta
    10321 agaattttat gtaggaaatc atcttcttat aaagaaaaga agcgcctttt aaataagttt
    10381 ccttatcaaa agacgctttc cgcgtggttc cacttttctt cataatcaat gattatctct
    10441 gacagctaac gcctgttata cgttcttggc tactttgttt cctataaaac agtttttcct
    10501 gttttctgtg tttcgccaag accactccca gaggcatttc ggcaaccatt cattgatacc
    10561 ttgcaccacc cggtatctct cttgtaatga tcagctgctt acttttttct gttcatcgtg
    10621 tttagttcag tacttgattt aaatgtgcta atgatttttc acgaccaagc aattcgatcg
    10681 tttcaggcaa ttctggacca tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa
    10741 gattttttcc ttttacgcca gtttcttttt gaacagcttt gatggcagct ttgacagttg
    10801 gagcatctac tacttccatt tgttctagct gtgctttgaa tgcgtggaga acagtaggaa
    10861 ctgtttcacc agcaagtact tcttttgctg cttcattcaa tacaggatgt tcattgaaaa
    10921 atagcgtaga cagatcaacg atttctgcag catagctcat ttgaggttga tacaggctaa
    10981 cgatcttctt cacccattcg attgtctttt catctgggtt ttctgctaca cgtccatcag
    11041 cgattagata aggcagacac atgtcagtaa atgcatctag atccattgct ttgatgtaat
    11101 ggttgtttac ccattgcagt tttttcgcat cgaaagctgc gggtgattta cttaggcgtt
    11161 ttggatcgaa cattttgatc aattcttggt gttcgaagat ttcgtcttcg cccacaggtg
    11221 accagcccaa taatgcaata aagttgaaca ttgcatctgg tagatagcct aattcgcgat
    11281 attgttcgat aaattgcaag attgattcgt cacgtttgct tagtttttta cctgtttcac
    11341 tgttgatgat caatgtcata tgtccgaagg ttggtggtgt ccagccaaat gcttcataga
    11401 tcatcagttg ttttggtgta ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt
    11461 tcatcaaatg atcatcgacc gcaacagcaa aattataagt tggcatacca tcgcgttttt
    11521 gtatgacaaa gtcgccaccg atattatcgg attcgaacga aatagcgcct ttcaccatat
    11581 ctgtaaatgc gtattctgta ttgcgaggca cacggaaacg tacaacagat tctagacctt
    11641 gtgcttcttt ttctgcttgt tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag
    11701 gcatttctcc tcttgcgcgt tgtgcttccc gttctgcttc taattcttct tctgtgcaat
    11761 agcatttgta tgctcgattg ctagcaagca gctgatcaat cagcggttga tagatttctt
    11821 tacgttcaga ttgacggtaa gggccgtatt ctccaggatt ttcaggagat tcatcccaat
    11881 tcattcccaa ccaagcaagg ttttccaact ggcttttttc gccatcttcg atattacgtt
    11941 tttggtcagt atcctcaata cgaataataa agtctccatc attgtggcgg gcaaacaaat
    12001 aattgaataa tgcagttctt gcatttccaa tatgcaggtg cccagttgga cttggtgcat
    12061 agcgtacgcg tactttcgtc attttcttcc tcttctttca aactagactt tgactaacag
    12121 caaaaattcc gtcagttacg gcttctcaaa tatcatttat ttaaagccac tcagcacaaa
    12181 ttttaccttt tctaaggtaa atagtaaagc caactggttg aatttcctta ccagttggct
    12241 gaatttcttt tttattttga ttgtttagtc tttttctctg tctctttttt attgtcgatt
    12301 cctcgtcctg catgtgcagg tttagcaaag atcatgcgtc cagcagctgt ttgaagggca
    12361 cttgttacaa cgacttcgat acgttcattc atgtaatgct gtccatcttc tacgacaacc
    12421 atcgtgccgt catcaaggta ggctactcct tgttggcgtt ctgtcccggc ttttactact
    12481 agaacattca tcgtttcacc agggatgacg actggtttta cggcatttgc caacgcattg
    12541 atattaagaa caggtacatt ttggaattca gagactttat tcaagttata gtcattggtt
    12601 acaatgacac catctaaaag cttagccaat ttgatcaatt tgctgtctac ttcactgata
    12661 tcttcaaagt ctccatcgta catttcgaca gatataccct cttctttttg caaggcattt
    12721 aggatatcta atccgcgacg accacgcaca cgtttcaaac tatcccctga gtccgcgatg
    12781 tattgtagtt cgtaaagaac aaaattaggg atcatcaagg ttccttctaa gaatccagtc
    12841 tttgtaatat cataaatccg accgtcaatg atcacgcttg tatccaagat tttatattta
    12901 tggaaatttt ctcctgattt tcgttctaac agttgactct caacttgact agctgtttct
    12961 tgctcgattt ttttgcttcg tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt
    13021 cccattctca atcccagata gcctaagatg atcatcagta aaatcggcaa tacactattg
    13081 acgaatggaa tttccatatt gtaaagaggg gtcgagatca caacacctaa agtcaatccg
    13141 atgatcgcac cgatcgcacc gaagagaaga taagtcaagc tgacctcact tagtttttgt
    13201 tctacttgtt tgataatagc tgagatatgt ttggccaaca gcaaagataa taaaaagaaa
    13261 ataagtgcgc ctaacaggct gttggtaaat gtattattca gccatccgtt tgactgttga
    13321 ccgattgcct gccaagctga gggtaaaaat gaaataccca agcttgcgcc gacaatgatc
    13381 atcagcaaag taatgacacg tttttgcatc gtttttcctc cgtttcgaca atcatttatt
    13441 agttagcgga acacacgctt caacgtttca gccaaagttg ctactccaac tatctcgatt
    13501 ccttttggcg cttcccagtt acctaagttg tttttaggta ggtatacttt tgtgaagccg
    13561 agtttttgga cttctttgac tcgttgttca atatgactga cacgtctgat ttctccagtc
    13621 agtccaattt caccgatgaa acattccgtt ggctgtgttc ctttttcttt atagcttgaa
    13681 gcaatactga cagcgatagc taaatcaatc gctggttcat ttaattttac cccacctgca
    13741 gcttttaagt aagcatcttg attctgaaga agcaacccag ctcttttttc cagcactgcc
    13801 atgatcagag atacccgatt gaaatcaaga cctgttgttg ttcgtttggc atttccgaac
    13861 atagtaggtg tgactaatgc ttggatctct accaaaattg gacgtgtccc ttccattgcc
    13921 acaacgatag cagagccagt tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga
    13981 ttagcgactt cttccaatcc atgctcatgc atttcaaaaa tcccaatctc attcgttgag
    14041 ccaaatcggt tttttactgc tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa
    14101 tataacaccg tatccaccat atgttctaac attcttgggc cggcgatcga gccttctttg
    14161 gtaacatgac cgacgataaa aatggctatc ccatttgttt tagcgatttt gagtagttct
    14221 gcagtggttt ctcttacttg gctgacactt cctgccacac ttgtgatgtc tggctgcgtc
    14281 atcgtttgga tggaatcaat gataacataa tcaggggaga tatgttcgat ggcacgactg
    14341 atttcgttca tatctgtttc agcgtataga taaaattctg tgttgatact tccgaggcgt
    14401 tccgcgcgta attttatctg ctctgcgctt tcttcgccgg atacatataa gacttttccg
    14461 cctatggctg ctaattgctg agaaacttgt aacagcaaag tggatttccc gatacctgga
    14521 tcgccaccaa tcaagaccat agaccctggc accacacctc cgcctaacac acggttcagt
    14581 tcttctaatt ttgttttgat ccgaggttct tttttaggga cgacgtctgc gatttttgtt
    14641 ggttttgctt tttctcctgt caaacttgtc cgagttctgc ggtccgttgt atcttgttcg
    14701 atttcttcga ccatcgtatt ccattttccg caatttgggc agcgtcccaa gaacttaggc
    14761 gacacatacc cgcaattttg gcaaacaaat tgtacttttg atttttttgc cattgtaccg
    14821 cctcctttta tcctaatcat gagacattaa atcaagctta agtatcttta acgttttatt
    14881 tactggaaga accaaatccg ccttgacgtt gagcagtcgt ttgatcttga tctgctttta
    14941 aaaacggcaa aaagatccct tggccgatac gctcgccttt tttgatcact acatccttga
    15001 aaccaaagtt ggtgaattgg aacatgatat ggccttcatt tgcctcatta ttgtaataat
    15061 cactgtcaat cacacctact ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg
    15121 aacggttagc aagctgcaaa aattcatctt ctcccatgta agctttgatt ccagtaggca
    15181 cgagggttgg tttcaattgt ttttcatttt ctgatacatc ttttttcaat ccatctgcga
    15241 ctgtttccaa cccctttttc cagcctgctt tccagatact tggaatcacg acctcttctg
    15301 ctgcttcaaa atcatagcca gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt
    15361 gatctttata tttagaaata acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DASHFF010000059        15163 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.059, whole genome shotgun sequence.
ACCESSION   DASHFF010000059 DASHFF010000000
VERSION     DASHFF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.059"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(9..884)
                     /locus_tag="U2I75_002109"
     CDS             complement(9..884)
                     /locus_tag="U2I75_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1697211.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1032..1364
                     /locus_tag="U2I75_002110"
     CDS             1032..1364
                     /locus_tag="U2I75_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1697212.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1476..2684
                     /locus_tag="U2I75_002111"
     CDS             1476..2684
                     /locus_tag="U2I75_002111"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN1697213.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2838..3293
                     /locus_tag="U2I75_002112"
     CDS             2838..3293
                     /locus_tag="U2I75_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697214.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3655..3930
                     /locus_tag="U2I75_002113"
     CDS             3655..3930
                     /locus_tag="U2I75_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697215.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3943..6021
                     /locus_tag="U2I75_002114"
     CDS             3943..6021
                     /locus_tag="U2I75_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN1697216.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6181..8067
                     /locus_tag="U2I75_002115"
     CDS             6181..8067
                     /locus_tag="U2I75_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1697217.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            8079..9026
                     /locus_tag="U2I75_002116"
     CDS             8079..9026
                     /locus_tag="U2I75_002116"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN1697218.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9046..9573
                     /locus_tag="U2I75_002117"
     CDS             9046..9573
                     /locus_tag="U2I75_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1697219.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(9636..10289)
                     /locus_tag="U2I75_002118"
     CDS             complement(9636..10289)
                     /locus_tag="U2I75_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN1697220.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            10421..11263
                     /locus_tag="U2I75_002119"
     CDS             10421..11263
                     /locus_tag="U2I75_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1697221.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11421..12317
                     /locus_tag="U2I75_002120"
     CDS             11421..12317
                     /locus_tag="U2I75_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1697222.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            12320..13033
                     /locus_tag="U2I75_002121"
     CDS             12320..13033
                     /locus_tag="U2I75_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN1697223.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13053..13571
                     /gene="msrA"
                     /locus_tag="U2I75_002122"
     CDS             13053..13571
                     /gene="msrA"
                     /locus_tag="U2I75_002122"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1697224.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            13568..13795
                     /locus_tag="U2I75_002123"
     CDS             13568..13795
                     /locus_tag="U2I75_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN1697225.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            13947..14498
                     /gene="lepB"
                     /locus_tag="U2I75_002124"
     CDS             13947..14498
                     /gene="lepB"
                     /locus_tag="U2I75_002124"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1697226.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(14581..15081)
                     /locus_tag="U2I75_002125"
     CDS             complement(14581..15081)
                     /locus_tag="U2I75_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1697227.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
BASE COUNT         5257 a         2603 c         3107 g         4196 t
ORIGIN      
        1 cttttttctt atagtttttt cagtaatctt gtctgtggct tcaaataagt aaagccttgc
       61 ccttccactt catgcacatt gatgattgaa aggaaagctt tttcgtcaat ctcgtggacg
      121 atcctcttag cttcagttaa ttcactagag ctaacgacca tatagatgat tttttttgaa
      181 ttaccagaga atgcgccttc tccagaaagg aaagtcacac ctctttgcag accggtcatc
      241 agtagtggtg caatctcttc gcttttgttg gagacgacaa gtagaccttt tgcagaatat
      301 ccgccatcta agatcgaatc aatcacacga ctgaaaacat acgaaacaat caacgtatac
      361 atcatacgtt tcaagtcgat ataggttaat gaagcaagta agacgatgat atcaaatgcc
      421 aatagggaac gccccatagt gatgccgtaa ttttgttcta aaatccgagc gataatatca
      481 ctgccgcctg ttgttccacc catgcggtag acgatcccgc ttcctacacc tgcaactaaa
      541 cccgcaagta gagaaacgat cagtagatca tgctgtaaat tgatttctag tggaaccctt
      601 tgccagatcc ataaaaagaa tgacaaagaa agtgtaccta gaacagtata ggccaaggaa
      661 cgtttaccga ggatctttcc tcctagtaaa atcaatggga tgttgattaa taaagtcgta
      721 taagctggat caataccaaa taaagctctt aagatcaagg tgatccctga gactccccct
      781 tctgctaaat gattagcgat gttcaagtaa acaagaccaa atgcataaat acttgttcct
      841 ataatgataa aaagaacttc ttttactgtt tttcgtttga ccatatgaat tccagctttc
      901 aattatgtat tttcacagaa tttccattta gaaattcctt cctaaatgta gcatggaaat
      961 tcgattttct caattgctct tatctaggat ttgcgttaaa gttttggtaa gatagttttg
     1021 gagggaaaaa gatggataaa aagcgttctt tatacagtat gcagcaggaa gtggatgaat
     1081 atatccagca attcaaggtg gggtatttct ctccattagc tcagatggct cgattaactg
     1141 aagaagtagg tgaattggca agagaagtca accacactta tggagaaaaa agcaaaaagg
     1201 cttctgaacc agttaactcg gtagcagaag aacttggtga tgttttattc gttacgatga
     1261 tcatggctaa ttcattgaat atcgacttaa ctgaagtttt tgaaaaaaac atggaaaaat
     1321 tcaatcaaag agaccataat cgctttgagc gtaaggacga gtaaacaaga tgaagtggtc
     1381 ctggccttga agactctgaa acggacttgt caaacagtca ccatgcaact gagtctttat
     1441 ctcaggacaa gaaacacgca ggaaaggttg gaaaaatgaa attaaaacaa ttacctctgg
     1501 aataccaaaa ggcaattcca gttctgaaaa aattagagaa tgcaggatat gaagcttatt
     1561 ttgtaggtgg aagtgttcgt gatatcttgc tgaaccagcc aatccatgat gtggatatcg
     1621 caacaagcgc atttccagaa gaaatcaaac agctttttcc aaaaacgata gatattggga
     1681 tcgaacatgg aactgtgttg gtattagaaa atgatgaaca atatgagatc accacatttc
     1741 ggacggaatc aacttatcag gactaccgta gaccagatca tgtcgaattc gttcgttcgt
     1801 tggcagaaga tttgaaaaga agagatttta cgatcaatgc gtttgctttg aaagaagatg
     1861 gagaaatcat cgatttgttc gatggtctgc aagatctaga agaacagatc ttgcgagctg
     1921 taggcaatcc tcatgagcgg ttccatgaag atgccttacg aatgatgcgc gggttgcgtt
     1981 ttgtcagtca actagggttc aaattagagg aagaaacctt ctacgccatc aaagaaaacc
     2041 acgagctatt aggtaaaata tctgtagaac gtataaatgt ggaattcatc aagatgctct
     2101 tgggaaatga acgggccgcg ggaatccatg catttattga aacagagtgc tacatttgct
     2161 gtcccggttt gaaagaagaa ggcgaagcac ttttacgttt tgctgaatta ccatccgtcc
     2221 cattgaaaga agagagccaa gcatgggcat tattgatcag ccaactggat ctaaaagaaa
     2281 atgagattcg ttcctttttg aaatcgtgga agtgttctaa ccaaatgatt aaagacgttc
     2341 aagcacttgt ccaaggacta cgacaacgag aaactggagc tttggaacca ttcgaattgt
     2401 acttcttagg caaaaaacat gcactgcaag tagaagaatt gatgatctat tttgacgaaa
     2461 cacctcagat ggatgccgta ggagaatcct atgatcgttt acctatcaag tcattaagcg
     2521 aattagcagt agatggtaaa atcttgctag aagaaactgg aaaaaaacct ggtaagtggg
     2581 ttagtgaagc tttgcaatta gctgaaaaag ccgtcgtaga acaaagaata aaaaatgata
     2641 aaaaacaggt tttggatttt ttatcgaaag aaaaattgat ttaaaaaaca ctagactgta
     2701 tctgctcagc tttgttggct acttaaagtg ggcagatacg tcattagaag aaaaaacata
     2761 agatgtgacc acttacataa atttgtgcta aactgaggta gtacaagaat aaattacatc
     2821 ataaaggagt ctttttaatg agtaaagaaa tgacagcgct aaaattttat tttagaaatg
     2881 gagaaacctg gacgatcaac cgccgtcata tcggcgatct ttggatcaaa caaatcacaa
     2941 caagttttgg acgaatcaat ggcagtgaat ttgttgaaat ccatccgtgt gctggattta
     3001 aaatcgaaat cttccatgaa ggggacgcag ttgcgacaca tgatatcaac ctaggcggac
     3061 tggaaatggg catgtttaat cgcgcattga aatacgaaga tatcgagcga atggaaatcc
     3121 tttaccgaaa tggcacacct gatcttgtgt acttcccata tctagataaa ggaacagaag
     3181 gattagacaa tcaatatcaa tcaacaaaaa tcagcgaaaa aacaggtaac ttgtatatcg
     3241 tgatcaatcc agatcaacgt gtagaagatg tatacgggga atttttcgaa taaatcgatt
     3301 tttacgcaaa aatgaggctg tgaaagagat gtgtcttatt actactgata ggctgtctct
     3361 tccacaccgt ttattcagaa taagggactg tgacaagatt tttgtcgcag ccccattttt
     3421 ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag taaacatcct tttgtcagaa
     3481 acttttatct ttcttaagtt ttacttgata tctgatacta tttggaatag aatgaggcgt
     3541 tgaaaaattt atggaaattt ctaactagca ataaacgatg aaacaatgaa atatttttca
     3601 tccaaaaaga actttgctat aatgatggaa gtttgtgtat aggagatctg gaaaatgaga
     3661 gagagttttg aacaacaaaa aaaactgttg catgatcgct atggcgccct ttcgatggat
     3721 gaccgccgtc aaatactttg taagttgcgt aaaagaaata tattgatgta tcgtcagttg
     3781 gaacgtctga aacatgatct gcttcgtttg gagtcaaaac gtgtacagtg tgaattagag
     3841 gggaaccaaa cacaggttga agtagtcgaa accaaaattt taaagaaaaa agaacaattt
     3901 ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga cgatggaatt tgctgagtta
     3961 gttattgtat ttgcagtaac gatcacctta tcgaacatag ctagccgcat tttgccaatg
     4021 attccagctc cactgatcca aattttcctt ggagtggtat taggtctgac agagtgggga
     4081 caatcaatcg attttgaacc tgaattgttc ttagttatga ttattgcacc attgttattt
     4141 agagaaggag aaaaagcaga tatttcttcg attctcaaaa atttcgggac aattttgttc
     4201 ttggcatttg gaggagtcat tttgaccctt gtcggggtag gtgcgacgtt atcattcctg
     4261 ttgccaagtg tcccacttgc tgcctgcttt gcttttggag cagcgttagg tccgacagat
     4321 gcggtagcag tcagttctct ttctggacga gtgaatatcc ctaaaaaggc gatgcatatt
     4381 ttggaaggag aaggtctttt gaatgatgca tctggtgtta ctgccttcca gtttgctctt
     4441 ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg ggatgagtct agtagtttct
     4501 agtattgggg gagctttgat cggctttttg cttgtctggt tcaaacaaaa aataattcat
     4561 ttgatcgaaa aagcatcagc acaagatgtc acagggtatc tgctgatcga acttttgctt
     4621 ccttttctag cctatgtatt ggcagaggca tttgatgtat ctggaatcat tgctgcagtg
     4681 gcagcaggta ttctccaagc ttctggtttt aggaaaatct ctgtgtttga tgcagaattg
     4741 tcgagcttgt cccacagtac atggacaact attgctttta cactgaatgc attagtattt
     4801 atatttttag gaatcgaatt gacgcaagtg ttctcgccag tatggggaga cggtttatat
     4861 ccaaacggtc ttttattagc gattatcgta ttaatctctg tcatgctttt tgtgatcaga
     4921 ttcatttcta tcagtctttt ttatgtattc aaagatggca gtaaaaagtt caaaaaacag
     4981 ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg gaacagtcag tttggctacg
     5041 atttttattt tgccattttc aatcaataat atgatgtttt accagcggtc tttgcttttg
     5101 tttctgacgg cgggtgtcat cttagtgact ttggtcatag ggatcattgt cttgcctatg
     5161 ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg ctttgatgat tttagaagag
     5221 gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc gtaatacaaa agaattttta
     5281 gccactgaag cagtgataga aaattatcaa gaacggatac gtgatctgta cttggaagat
     5341 ttaaccgatg atgaaaagca agaagtacaa gaaatacaag cactgatttt gtcgatcgaa
     5401 cgggatggat tggatgaaag ttatcgttcg ggcaagcttt cttctaatgg ttatcgtttt
     5461 tactcccgtt ttctttctcg attcgaacat tctatcacaa gccagatttt gtcttttatc
     5521 ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg ttctccatcc gaaaatgttt
     5581 tggcaaagaa gatatgctga tcgtcagaca tttattagtg atgaagatat ccaagaaatc
     5641 agagagacat atcaaaaaaa tactcagttg attattgaaa gtttagataa cttgactgat
     5701 atttatgatg aaacgatggt tcaatttttt atccagcaac gcaaacttga aggaataaaa
     5761 atgaactcgg ggaatttgat ttcttcgtgg atgatccagc aagatagtct gtttacgaaa
     5821 aaaatgctaa gaggttatta tcttgaaaga aaaatcattg atgaatacga agttgctgaa
     5881 aaaatcacga cgttttctgc gaacagctat cgcaggaata tcaatttgtt agaatcttat
     5941 acgatgaaca aaccatctga taacttttct ttccgatttg cgtttcgagc aaagtcaaaa
     6001 aaaacgagtc caataaaata aaaaggtaaa aattttcaag ttttataaaa aacaaaacat
     6061 atcagaacag gatcgaatcg gtcctgttct ttttttgaga acacaaaaag aacaaatacc
     6121 aaaaaaattt tcctgaagtt cactattttt atgatacaat atccaagata aggagggaac
     6181 atgttgaaag aattaaaagt aactgacctc aaaaaaactt atggtgaaaa agatttgttt
     6241 gaccagattt cttttttggt ccacgaaaaa gaccgtatcg ggttgattgg gacgaacggg
     6301 acgggtaaaa caagtctctt aaatattatt gccggtatcg atagcagcga cggtgatcgg
     6361 caaacagtgt tttatccaac tgattaccgt attggttatc tttctcaagc agctgaattt
     6421 tccgatgaat taactgttct ccaagcagtc tttcaaggaa acagcccctt gatccaaact
     6481 gttcgagcat atgaggaagc attgatcgaa ctaggggaaa acggagaaga ccctgatgtg
     6541 caaaaacgtt atgctaaagc agaagaacaa atgaataaag aagatgcttg gactacagat
     6601 accaacgcaa agattatttt gcaaaaatta ggaatcagtg aattagataa aaaaatcagt
     6661 actttatcag gtggtcagaa aaaacgagtt agtctggcgc aagtgttgat tgatgaaccc
     6721 gatttactat tactagacga accaacaaac catttagatt atgaagcgat cgagtggttg
     6781 gaaaactatt tgaaacaata ccgtggcgca ctattgatgg ttacccacga tcgttatttt
     6841 ctagatagag tagcaaaccg aatcttcgaa ctttcatttg gaaaattata cgagtataaa
     6901 ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg atcgtgtaga agtagaacaa
     6961 gaagaaaagc gcaagcggct ttataaacag gaattagctt ggatgagggc tggcgtacag
     7021 gcacgtggca ccaagcagca agcacggatc aaccgtttcg aagatctaaa ggagaacttg
     7081 taccaagtaa accaagaaga tgatttagag ctgaacctag cgacacaacg tttagggaaa
     7141 aaagtactgg aaatcaaaga tggcagttac cgtataaatg accaaacact gctagaacat
     7201 ttagatttat tgattcaatc tcgggaaaga ctaggtatta ctggtaaaaa tggtgcggga
     7261 aaatcgacat tgcttaatat tctagctggc agaattcccc tggatagcgg tacaatgtct
     7321 atcggtgaaa cggttcatct tgcgtattat acacaagaaa atgaagaaat ggcaccagat
     7381 aaacggatga ttgcttactt acaagaagct gctgaagaag caaaaacagc agatggcagt
     7441 caaatcggag tagcagaatt acttgaacga tttttatttc caagatttat gcatgggaca
     7501 ctgataggca aactttcagg cggagaaaaa cgccggttat ttttgttgaa actgctgatt
     7561 cagcaaccta atgttttatt attggatgaa ccgaccaatg atttggacat agcaacattg
     7621 acgattttgg aagattattt tagaagtttt ccaggtgctg tgatcactgt ttcccatgac
     7681 cgttatttct tggacaaggt agcagataag ttattagtat tccaaggaaa cggaaaacaa
     7741 gaactttatt acggaaacat gtccagttat ttactgaagc aaaaagagac ccagcagcct
     7801 gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg ctgaaaagaa gaaattatct
     7861 tacatggaac aaaaagaatg ggaaacgatt gaagatgaga ttgcagaact tgaagaaaaa
     7921 atctcattgc tccaagaaga gatgaatcat caaggagata atttcactcg attacaagaa
     7981 ttgcaaaacg acgtatctga aacagaagca caacttgaag aaaaaatggc acggtgggaa
     8041 tacttgagtg aatgggtaga agattaggag gaaaaaggat ggaacaagcg tatcttgact
     8101 taggaagaaa attgttggaa gaagggcatc gtaaagaaga cagaacaggc acaggcacat
     8161 taagcttatt cggataccaa atgcggtttg atctgcaaaa agggtttcct ttgttaacaa
     8221 cgaaacgagt accttttgga ttgatcaaaa gcgaattgct ttggttttta aaaggagata
     8281 cgaatatccg ctatctatta caaaataaaa accatatttg ggatgaatgg gcatttgaac
     8341 gctacatcaa aagtccagac tataatggac cagatatgac tgattttggc catagatgtt
     8401 tgacagatcc tgaattcaat gaagaatata aaaagcagag caagcttttt tgcgagaaga
     8461 tcctaaccga tgattccttt gctgaaacat acggcgatct tggacacatc tacggctatc
     8521 aatggcgtca ctgggaaaca aaagacggtg gctttatcga tcaaatcaaa caagtgatcg
     8581 aagcaatcaa aaagactcct gattctagaa gattgatcgt ttctgcatgg aatccggaag
     8641 atgttcctag catggctttg cctccttgtc atacgatgtt tcaattttat gttcaagagg
     8701 gacggctaag ctgccaattg tatcaacgca gtggggatgt gtttctaggc gttccgttca
     8761 acattgcaag ttatgcgttg ctgactcacc taattgcaca tgaaacaggt ttagcggttg
     8821 gcgaatttgt tcacacatta ggagatgctc atctttatat caatcatatc gaacaaatga
     8881 aagaacaatt gagccgagaa atacgcgcat ttccgacttt agttttgaat caggaaaagc
     8941 agtcagtatt tgattttgag atggaagata tccaattaga aggatatgac ccacatccgg
     9001 cgatcaaagc tcctatcgct gtttagaatc agaaaggatg agaaaatgtt agctgcaatt
     9061 tgggcacagg atgaaaacgg attgattgga aaagaagacc gtcttccttg gcgtttgcca
     9121 aatgatctga aattcttcaa acaaatgaca gaagcaaata cactagtgat gggacgaaaa
     9181 acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc agacgattgt tttgacgaga
     9241 gattcttcct ataaagcaga aggcgtgcat gtcatgcata gtgtggaaga cgtgcttgct
     9301 ctggaaaaag aaacagacgg tatctttttc atcgctggag ggtcagctgt ttatcaagaa
     9361 tttctgccat tttgtacgat attataccgt acagtcatcc atcatgtatt cgatggagat
     9421 gcttattttc cacctgttga ctggtctgat tggtcactga tcaatactag tcaaggagaa
     9481 atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt atcagcgtaa tgagaatcct
     9541 gtaaaaagcg ggacaggcga attgacgata taaaaaagct atggatagac tagctgttat
     9601 ttagagctgt cccatccata gctttatttt ttttattaag taaagaataa aacagagaag
     9661 tacataaaac ctgcacctaa cataacaaac aaatgccaaa caacatgcat atagcggata
     9721 gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa gcccgcctgc taccatcaaa
     9781 ataacaccag tcattccaag agagtgataa agaggaacag cagccacgac acagagccac
     9841 cccataaaaa tatagatgat cgtagatact tttgatactc tctcccgttt atgcaacgtc
     9901 aacgctttat atataatgcc aagaatcgca aacaaccaga ttaatacaaa taaagtccaa
     9961 cctagccatc ctcggatact cagcaaacaa aaaggagtat aacttcctgc aatcaaaaga
    10021 aaaatcgaac tatgatcgaa tacttggaac accttttttg cttttgtaaa aatcaatgaa
    10081 tgaaacaaag tagacgttaa aaataaaaga atcatcatcg atccatagat agcataagag
    10141 acaatatgga gggaggaatg caagtgggca ccttttacta aaagaaccac tagtcccgcg
    10201 atactcaatc cagtcccaat tccatgggtg actgcattaa acacttcatt taatatttca
    10261 tacgttcgat taaatttcgt tttttccatg aaaacggctc ctttactagg tgtaattaat
    10321 gaaaaatcat tatttgtctg ttatactaaa aatagaaatt gtcttacatg tagtttaaca
    10381 tgaataaggc atcttgttaa agaaagagag agagaaaata atgacaaatg taaaaatcgt
    10441 aacagattct tcatgtacga tggaaaaaaa tgtacgcgat gctttaggca ttcgtgtcat
    10501 gccgctatcc atcatgatcg acggaacagt atatcccgat gatgaccaac tagacggcga
    10561 aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc aaaacaagcc aaccaccaat
    10621 tggagaattc atctcgatgt atgatgagct aggagaagac ggcagtgaag tgatttcgat
    10681 ccatatgaca aaaggcttga gtggaacagt agaagcagca agacaagcca gtaatctaac
    10741 aaaaacgaaa gtaacggtta tcgacagtga taccactgac caaggattat cttttcaggt
    10801 aattcgagca gctgaattag caaaagaagg taaaacagta gagaaaatcc ttgaagaagt
    10861 caaaaaagta caagacaata caaaattata catcggaata tccacactag ataacttggt
    10921 aaaaggtgga agaatcagcc gaacaactgg tttgctgtct agtgttttga acatacgcgt
    10981 ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact aaggggcgag gaaagaaaac
    11041 gttcgataaa tggtttaata atttgaaaaa tgaactgaaa gaattgccaa atgtgcgaca
    11101 aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa gaattcaaag agggtttgca
    11161 agaattattc ccggacatgc atatccctgt actgcatacg aatccggtta tcgctacgca
    11221 tactggtaaa gacgcgtttg caatcatgta ttatgtagat tagtcgaaaa gaggataagc
    11281 cctcagcctg aaaggcttgc agcttattct ctttttcttt ataaaattag gaaaataatt
    11341 gttttctttt ttcataaaga ctaccttttt gataaaattt ttgataacat tagaaccaat
    11401 cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca tttgatcctc gttttgttcc
    11461 cattattaat tgcgatcatc atctatgcag gtttagctgc ttctataccg aaagctggcc
    11521 cactaatgaa accagtaact actgtgagag aagatccaaa taaaaaagaa aaacttcact
    11581 acatagctat cggagattcg ctgactgaag gagtaggaga tcaaaccaag caaggaggat
    11641 tcgttcccct tgtcgcaaat gatctaaaag accgttataa tttgaccgcg gttgaaacag
    11701 aaaactatgg tgtaaatggt gaacgaagtg accagatctt aaagcgagta aaaacaaaag
    11761 aagaaattcg aaacaatatc aaaacagctg attttatcac actgactgtt ggcggtaatg
    11821 atttgatgaa agttatccaa aataatttat tcgggttaac ggtcaattcc tttaaaaagc
    11881 cactgaaaaa ctatcaggaa aatgtcacag aactattgga agaatttcga tccctcaata
    11941 aaaccgcacc tatttacatt ttaggcatct acaatccatt ttatttgaac tttcctgaga
    12001 tcactgacat gcagacaatt gttaacaatt ggaacaaagg aacagaaacg gtgacaaaaa
    12061 aaatgccgaa ctgctacttt atcccaatca atgatttgct ttatcaagga ttgaatcagg
    12121 aagtaggtgt cacagaagaa ggaacagatc aaacaactag cacgacagaa aatctaaatg
    12181 gcatcaaaaa taatgtgctc tatgaagaag atcatttcca tccaaataat ttaggttatc
    12241 aattgatggc aaatgcagtg agagatcagc tgatcgagac acaaaaccaa tggttagaga
    12301 aggaggatga tcaataaaca tgaaaacaag atctgacgta tcttcaaaca aaacaaagaa
    12361 aacaaattac tggaaattcg ccttccttat ccttcttggg gtagtacttg gttcgaccgt
    12421 atttttaggc agccgtattt ttgctaatcg ggaaccagaa ctcccagaaa taccagctct
    12481 tacggaaaga caaggagatc ctgtcttaac gatcaattca aataaagaaa aagtcaatca
    12541 aatcatcagc tttttcctta gtgagtatca aaaagatagt gatattgaat ataaatttta
    12601 tttggaaaat gaagcactac tcaatggtac tttcgaagta ctaggttttc ctatcgattt
    12661 ctatttatat tttgatccgt atgtgatgga aaacggaaat gtccaactaa aagctagaag
    12721 tctttctatc ggaacattga gtttgccaat caaagatgtg atgaatatga tcaagagaaa
    12781 ctataaatta ccagattgga tcgaagtcaa tacagatgat ctgactatca tgcttaggtt
    12841 ggatcaattc cgaatgcaaa acgggatgta tattaaagca gataaaatcg acttagtaaa
    12901 tgatgacatt cggtttagtc tatatttgcc aaaagatacc tctgatgctt caaacaactc
    12961 aaataccaac aaaactagca ccagcagtac tgaaagcaag acaaaagaaa ataaaaaatc
    13021 atcggatgag tagtattaga gggggaatca taatggaacg agcaatattt gcaggaggct
    13081 gcttctggtg tatgatccag cctttcgaca cgcttcctgg tatccataca gtgatgtcag
    13141 gatatactgg agggcatgta ccagatccga cttatgaaca agtaaaaagc caaacgactg
    13201 gacatacaga agcagtggag attatctatg atccaacatt gatttcatat gaacaattag
    13261 tagaacttta ctggcagcaa actgatccaa ctgacgcttt cggacaattc gaggatcgcg
    13321 gtgacaatta tcgcccggtg atcttttatt tgacagaaga acagcggatc attgctgaaa
    13381 aaagtaagaa acagttagct gaaagtggac gtttcgaaca gccaatcgtt acaaaaatcg
    13441 aaccagccca accattttat ctagcagaag aagaccatca agagtattat aaaaagcatc
    13501 ctgaaaattt taaaagaaac catgcacgcc gggctgcctt tatcgaagaa aattggagga
    13561 acgaagggtg aatcgaagct tttttcatta tttaatgaca ctgcgaggcg gtggcacaca
    13621 tgatgcttta tctgttttcg ctacagaagc cggtaaagac atccaatttc ctaagcacag
    13681 ctctagctac gaagaaattt ctaattatct ggaactgaat gtcgactatt tgccgtccat
    13741 ggatatcttt gacgaagcat gggaaaaata tttagaaaat aatcatgaca tataagagat
    13801 agttttgtac tttatgatca aaaaagtttt ttgtccaata gcctaattga aagaattatt
    13861 tttatttcag ctatctagtc gcattttttt gatatttttc ttataataaa gaaagaatga
    13921 aaaaaagagg gatggattga tagagattga caaaaaaaca acgctatatt aatcgcttct
    13981 ggcttgtatt caaatactta cttgtttctg tattccttgc gttcatgttg cggggatttt
    14041 tattcatccc ggtaccagtg gaaggcaatt caatggaaaa cgtgttgaag caaggtgata
    14101 tggttgtgat ggaaaaattt tcggagatca gacgatttga tattgttgtt tttcaattag
    14161 cagatggcac gatttatatc aaacgagtaa tcggtcttcc aggagaaaat gtcagctacc
    14221 aaaatgatca attgaagatc aatgggaaag tagttaagga accttatcta actaaaaacc
    14281 tgaaatcaga tcatgcaaat gcatcctata cgacggactt tacactacaa gagctaacgg
    14341 gacaatccaa gcttccggaa gataattatt ttgtacttgg agataatcga agagtttcaa
    14401 aagatagtcg ttcgtttggc acgatcaata aaactgatat tctaggtaaa gcaagatttg
    14461 tctattatcc acttgatgaa ataaaatgga tccagtgaga aacaataaag cgtgcagtct
    14521 gttgagactg cacgctttat tgtttaatcc aaaatcagtt tactacctta gtacgtaaaa
    14581 tcaaacagct cgtttaaaca cttttctagc ctctaaagtt ttgataaatg aataaacaac
    14641 cattatttga atagggattt ggatcatatt cctgatgaat ctacctagaa aaattacttt
    14701 gaaatctgca ccaaacaaaa cagacatcag ccaagaattg ataaaaaggc tgactgggat
    14761 cataatcaat aattgggcag cgatgacgga aggcaggttt atctttttat gatataaaaa
    14821 taagccatta ataaatccac caaggaaagc cgtgataaga aaagcagctt gaaacgttcc
    14881 tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt tcaagcaaac cagcccagat
    14941 cggtccatat agataaccca taagcgccat acttaaaaat ccgaatgtga tttgaaaata
    15001 agcaggacca atcgctatct tgtataagat gacatcaagg gctaataaaa gagcagaggt
    15061 cgacaacaat cttgcattca tttcgggcaa tctaaaaacg tcttttgcaa tcaaagtgtt
    15121 caaaatattc tctcctttgt cagttaataa taaaaggata cca
//
LOCUS       DASHFF010000060        15160 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.060, whole genome shotgun sequence.
ACCESSION   DASHFF010000060 DASHFF010000000
VERSION     DASHFF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.060"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            151..1938
                     /locus_tag="U2I75_002126"
     CDS             151..1938
                     /locus_tag="U2I75_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HEN1697228.1"
                     /translation="MDTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLE
                     QADQTGDYSKLTPNNLVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQ
                     YGATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHES
                     LLNQRIVVEFYHKGKKFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAW
                     YEFSQKVLKDEEGKIVYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPM
                     AIFMLTPDYDPSNHIIVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMD
                     NMEGIMTVTAGRNMLWDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEE
                     FSKKVGNKTVEQENSTINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQ
                     DLKGHKIRPYPIFNHGETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYD
                     YFLPDEYARNLYHGLHDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQE
                     YVEKLIKNEEISEMIRKRLLLEAEKAVKE"
     gene            1935..4313
                     /locus_tag="U2I75_002127"
     CDS             1935..4313
                     /locus_tag="U2I75_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697229.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            4374..4916
                     /locus_tag="U2I75_002128"
     CDS             4374..4916
                     /locus_tag="U2I75_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697230.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            4994..5326
                     /locus_tag="U2I75_002129"
     CDS             4994..5326
                     /locus_tag="U2I75_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697231.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            5327..5920
                     /locus_tag="U2I75_002130"
     CDS             5327..5920
                     /locus_tag="U2I75_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697232.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            5942..7636
                     /locus_tag="U2I75_002131"
     CDS             5942..7636
                     /locus_tag="U2I75_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEN1697233.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            8324..10201
                     /gene="ltrA"
                     /locus_tag="U2I75_002132"
     CDS             8324..10201
                     /gene="ltrA"
                     /locus_tag="U2I75_002132"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1697234.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            10332..10709
                     /locus_tag="U2I75_002133"
                     /pseudo
     CDS             10332..10709
                     /locus_tag="U2I75_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            10709..11914
                     /locus_tag="U2I75_002134"
     CDS             10709..11914
                     /locus_tag="U2I75_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1697235.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            11930..12574
                     /locus_tag="U2I75_002135"
     CDS             11930..12574
                     /locus_tag="U2I75_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697236.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            12585..13391
                     /locus_tag="U2I75_002136"
     CDS             12585..13391
                     /locus_tag="U2I75_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697237.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            13413..13643
                     /locus_tag="U2I75_002137"
     CDS             13413..13643
                     /locus_tag="U2I75_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697238.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            14189..14464
                     /locus_tag="U2I75_002138"
     CDS             14189..14464
                     /locus_tag="U2I75_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697239.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            14506..14928
                     /locus_tag="U2I75_002139"
     CDS             14506..14928
                     /locus_tag="U2I75_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697240.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            14947..>15160
                     /locus_tag="U2I75_002140"
     CDS             14947..>15160
                     /locus_tag="U2I75_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697241.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKT"
BASE COUNT         5594 a         2349 c         3117 g         4100 t
ORIGIN      
        1 aatgatccaa aaacagaatt atatgttggt agtaaagatg aactagaagc tagagggtat
       61 gatgtatatg cctttaatat caccgatcca ctgcaatcaa tgagtgataa tccattggca
      121 ctcattgtga aatactggaa gcgaggagac atagacaccg caacacagtt aaccaatact
      181 tttacgaata cgatttatta tgatgcaaat gcttcagaca ataaatactt taacgaaaat
      241 gcgcagaaag ctgtcaatgc gcttattttt gcgttattgg aacaagcgga tcaaacaggg
      301 gattactcca aattaacacc aaataatctc gttgaactac tttcagaaat tggcggtttt
      361 aattattatc cagatcctga gaatgaattc aagcagctca atgccttaga tgagttcatg
      421 aatcagttac cccctggaaa tatagcaaaa aaacaatatg gggcaacaaa aattggatct
      481 gataaagcga aagggaatat cttgtctacg gcaattacag gattgaatcc atttacccta
      541 acgaaaattg cgaagatgac ttctcaatcg accattgatt acaagtccat tggttttccg
      601 aagtatttgg atctcaaatt acatgaaagc ctattaaatc aacgcattgt cgtggaattt
      661 tatcataagg gaaagaaatt tcacgaggaa cgagtcaaag ttggctatcg tggtttttgt
      721 gaaatcaact ttgattgtca tttagagcag ggagatcatg tttgcttaaa atacaatcat
      781 caaggaaaac aatatgttgc ttggtatgag ttttctcaaa aagtattgaa agacgaagag
      841 ggaaaaatcg tttatcgaaa aaaacgagga gaaactcatt tgccagaact agaaaaagaa
      901 gccgttctaa cattagatga gaagaaaagt aacctattag tagagtctat tcggatgcac
      961 tataccgata aaccaatggc tatttttatg ttaacgcctg attatgatcc atctaaccat
     1021 atcattgtta gcatctttct tagtcagttg tataaagaat tatctacgca atgtgttaag
     1081 actaaaggag ataaatgtca ccgtaggatt cattttattt tagatgaatt tggaaatatg
     1141 ccaccgatgg acaacatgga aggaatcatg acggttacgg caggtcgtaa tatgttatgg
     1201 gatctgttta ttcaatctta tcagcaactt tatgcgaagt atggagaaca aaacgcagcg
     1261 attatcaaag aaaattgtca gacacatatt tatattatgt cgacagatga taataccatc
     1321 gaagaatttt ctaaaaaagt ggggaataag acggttgaac aagagaacag taccatcaat
     1381 tcattaggta tgaatactca tatcaatcgc aatgttgatt cagatcgtat cttaactcct
     1441 gaaagaattt ctactttttt agaaggtgaa acgattgtac tcgctccttt aaaacgacaa
     1501 gatttgaaag gtcataaaat acggccgtat cctattttta atcatgggga aacgaatctt
     1561 ccttatgctt atcaatatat tgaagatttc aatccatcgt gggatatcaa tgatatagac
     1621 atacattgta cacatgcgga cttggatctt aaggctttac aaattgatta tgattatttc
     1681 ctaccagatg aatatgctag aaatctctat catggactgc atgatcaatt agaagaaaaa
     1741 aacgaagaaa atatgtttta taaaatcgtt caatcttttg agaatgagac ggctaaaaaa
     1801 ttgttaattc aattttacga agaacaagca acggatcgtc ttcaagaata tgttgaaaag
     1861 ttgatcaaaa atgaagaaat ttctgaaatg attaggaaac ggctgttatt ggaagctgaa
     1921 aaggcggtga aagaatgatc attggaggaa ttttctctgg catagccgac tggtggtcag
     1981 gtttattcgg agaaggggat gcagatccca aaaccgtaac gaaagtctta caagattatg
     2041 gtaattatct gcagtatcaa gatgtattca gttggatttg taatactttt ttatggggat
     2101 tgatcaaagg gttatataat ctcaatactt tattagaaaa aacaatttat caaagctttg
     2161 atttaaaaga cttattgaac gccgctggtg ttaatgaact atttcaaagt ttgattagta
     2221 aggtattggc tcttttttgt gtcgtcacat taatttattt aggcttgaaa ttttttatga
     2281 gtaaacaccc accgaaagtc aaaaatatct tggttaattt atttatggcc atgatcttga
     2341 tttttggtgg tagttcttta atcgatgaag ggttaaatat cagtaaaaac ttttatggag
     2401 acgtcactgc agcaaataaa gcagataaaa cgggttcacc tgcttttcaa attattcaaa
     2461 ataatgtgat tgatgtcagt acattactag cgactgaccc gaataaagta gaatccttac
     2521 caacagataa aagaaatgct ttaacagcga aaaatttcaa aacagcgaat atcaatggga
     2581 ttattacacc tgatcaagct aagaaaatgt ctgaatcaag tgatattagt acagtacaaa
     2641 aagatcggtt aaaggcatta cagtatcgac tagagttaga cgataatggg aaagaaatcc
     2701 ctgttcaaat tagtgatgaa ggtttagcaa aatatgtgta tacgtcaggc tatcgtcgat
     2761 acattagtag tccagggatt attttggctg gggaatcttc tttgtcggta gcctatttat
     2821 ttattttgtt tacaatcgtt acttgcatta ttgagctagt atttaaaaaa ttctatttag
     2881 taattgccgc gtcaacggac tttgagacag gacaacggat gaaaagtgcg atagaagata
     2941 tttctcaatc cttcttgtta ttggcgttta cgattttaga attacgaatc tatacattaa
     3001 tgctttcagg tatcggtgat ttgcatgcgg taggaaagat caatggattc ttgtatgttg
     3061 ttgctttaat tgtgctaact attgcgctat tcaaaggctc tcaatcggtt acgaagattt
     3121 tcggtgttga tacgagtctg aaaaacggca gcaattctct tcttagcatg tttgccttag
     3181 gaaatattgc gaaaaatgtg ggtagcggag ctaaaaacct tgcaagtgct ggtaagagtg
     3241 gtttaagcca tttaaatgat attcgtaaga atggatttgg ccatcgaaaa gatattaccc
     3301 atgataacgg ggaagaacaa tcagacgata aagctacaac tgtacctaat agtaagaaga
     3361 cagtttccga aatgttcggg aaagcgaaaa atggtgtttc taaagccgca gaaggtcttg
     3421 gttacgtcaa tgaacgtggg ttaaaaggaa cagttgaaga tcttacagaa aaagccaaag
     3481 acaccttaga agagaaatta gacggcacga gagctaaaga atttgctgaa gctgtgaaga
     3541 atccatccga agcactagaa aaacaaaaag aaaaagcttc agcagtcaaa gaaggactaa
     3601 aagatgcgta tggtgaagga caagtcaatg cagcagtgaa ggcaaataca acaagtgaaa
     3661 aagaaaatca agaaggtgca gcgattcctt cagccaaaga tcaccaagat gagttacatt
     3721 ctaacgaaaa ttcgatcgga gaaaaagatt cagaagaagg acaacatggt cagttaccaa
     3781 aaaccgatcc agaacaatat cgttataaat ctaacattaa ttctgaaaac gaaaaacgca
     3841 tttcagattt accaaaacga aatcaagaag tacaaacgga tgaggattcc atcgatctgc
     3901 caaatgataa tggcatcgaa gttggaactg aaaaatcaac gaaaattggt ttgcctaaga
     3961 cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaagagaat gatcaaagct
     4021 ttgttcatca atcacctgca ggtactgttc aaatgaaatc aagtcgtttg cctgaaaatg
     4081 gaaatctgac aactgtttct tacaatttag ctgaaagtgg tgaaacagag aatgcgtccg
     4141 ttcaaaatat tgtgacaaaa gaagctgcac ctaagccaat caataaagtt cataatggat
     4201 acagaccagc aaacgtagca tttactgcac cgacaaaaat aaatgtgaca agagaaacta
     4261 aaaacaggaa caatgaattg aaagaacaac tattgaagga tatcaaacca taaaaggcct
     4321 atttaagggc ctttttcttt ataaaacaag tttaaaatgg aaaggagaaa attgtggagg
     4381 atattttaat agctacgttt ataactgcac tgggagcatt tttaacgata tttgtggtga
     4441 ctattaaagg atttaaagat acggatagtt tacaaaaaga tcatcagtct attttagatg
     4501 gtcaaaaaac acataccgaa agcattattg tacacctttc agaaggattc acttatttga
     4561 aagatgctat gcccacattc acagtaaaat taacggaaat atttcattct gttgagcaaa
     4621 tatcgtcaga attgaaagag caaagacaac aaaaagatcg tcaacttggc tcacttaatt
     4681 ttaggcaaga aacgatacaa gaatcattgg agaatgtaaa tcgattaaac gatgaaatgc
     4741 aacgagttca tttggaaaat gaagaattac gcgcacaaaa ttatgattta attcaaaaga
     4801 aaagaaatct ggaacaaaaa attgttgaat taaaagaaga aaatcgtcaa ttgaattatc
     4861 aattacgacg aggaaaaaat cgttctcgtg atttaggaaa tgaattagag ttgtaatatt
     4921 tagaaaaacg ctgtgagaaa tcacagtgtt ttttatttct aaaaatacaa aatataaggg
     4981 gtatggttat attatggata aatacattca tatgaaaaat cctccaagat ttagtgggga
     5041 atggaagttt ttcgcctttt tctttttaga tctattaacg cttggtggct gctttttttg
     5101 ggcgaaaata gtcaataatc ttgtccactt accactcttt tttgcactct tgaattatgg
     5161 attatggctt gcgatagcgt tagtgattgt ttggcgaccc tataataacc ctggtcaacg
     5221 ccaaattttt gcgattatcg aatctatttt ctttacggat aagaaccact atcatgcgtt
     5281 agatcctaac cgaaattatc atggtacgta taaggaggat gaataaatgc gagtcaaagg
     5341 acaaaattca gttgataaga aatccatttt gcagtttgga aaaccagaag agcgctctat
     5401 cttggactta tttccatata aatcctgtac agaagaggga gtcttaatta ctagagaaga
     5461 tcattttcag cgttattatc gtgtcgtttc cactgacgtg gagggattaa atgaacaaga
     5521 gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt acgtatgtgc caacaattaa
     5581 gttagttagt ttgaccacag agacggatct aacggaacaa atcgtgcaaa agcggcagtt
     5641 attagataaa aatcgtatgg aacagtcact aggaagaaat ctacagcgat taagaaaata
     5701 tgaaaagaaa ttagtagagg agatccagga attaaaacgg gcagaacaag aacgtcctga
     5761 cttaagcttc tttttcgtta ttgaaggaaa gacaatcaag gatttacaaa ctagaaatcg
     5821 tcagttaatg cgatctagtg gcattttagg gttaaaacca ctctacaaaa aagaattgat
     5881 taagattctt tatcgaatga acaacatgaa tgacgaataa aaaagatgga ggaaatgatt
     5941 catgctagat gtgataaaaa cagctttttc aacgcaaagt aatcaaaagg tgattgatcc
     6001 attaaaacaa cttggtaatc gtgaaataga gattttaaaa tcaaaaggtt atgatttaga
     6061 tttcttaaaa caagtgatgc cacaaggtgg gttgaatttt gatgaagaat atgtgaccta
     6121 tggaaatggt tattcacgat gtgtgacagt ttatcattac acaaaaaatc ctactctttt
     6181 ttggttggcc aatctcatga acaatcctca tacagtggcc acactagatg ttagcacaga
     6241 tgaaaagcaa aaagtgattg atcaaatcaa ccgcgcatta gatgaattaa atgatcgatc
     6301 agataatgaa cgaaaagcga ctgaccgaaa taaaagtgcg gaagaatacc tggaactagc
     6361 ggaatatgcc cgaaaattga cttctaatgg ggaaatttct aaacaagtca aagtgagagt
     6421 acatgtttac ggaaatacga tccaagaagt ggatgaacgg gcgcaggaac tgatcgaaaa
     6481 tctaaaaggg atggattatc gtgcggtgac ttatctgcat aaaaccaaac gagattggca
     6541 atcgatgttt accatgttcg atgaacaagc tgaatggttt ggaaatatca aagggcaatc
     6601 acttccatcg ttaaacattg gcggaggatt cccgttccac caccaaacgc taaaagatcc
     6661 aggaggcaca caatatgggg aaaccatgac aggcggccca tttgtctgga atgccacgta
     6721 taaaaatagt gtacgtttat ctaccaatac cttgcttcta ggaatgatgg gagctggtaa
     6781 atcgaccgtt cttaaaatga tcgcggaagc ccatttagct gcaggagact ttatttgggg
     6841 ctttgaaaag ggaaaagact ttattccttt cttaaaggaa tataacggta ttatggtgcg
     6901 tttagacgga tctgacggaa tgatcaatcc tttagagata tttgccacac ggacgtatga
     6961 tgaagcatcc agtttatatg acgatggatc agtcaaagat ttgaagatca atgaagcagc
     7021 gtcctatcaa acacacttag acaaagtggt ctatcaggta cagttagtga gtcctcagct
     7081 aaaaggaacg atgaaagcag aatttaagac ctacttaaat cgtttttatg aagagtatgg
     7141 aacagtacca cgcggcttta cttcctcaaa ttcgcgttca aatactgata cgcaagtcac
     7201 agggaaagat ccagaagctt atccgacgtt caaagaattt ttagcctttt taggacaact
     7261 ggaactacca ggggcttccc aagagaaaaa gaatcggaaa gaagaaatgg agtctatcgt
     7321 ggaatcctta tgtgagactt atggcatgat ttttgatggt catagtacga ttcgacacct
     7381 tgaccaacaa cagttggtct tttttgacat tgactctatt acaggcctag atcgaggggt
     7441 acaacaatgt cagatgtaca tggccatgac gttgatttgg aatcaggcat tgattcaagg
     7501 acgaagacaa cgacgcttaa ttaaagaaaa acaaatggaa gaacaagact tcaaacgctt
     7561 cttggcgatg attgatgagt gccataatgg tgcgacctgt tctgcagtga aaagctgtgg
     7621 gttgcaagta gtctaaaaac tattgtaata actcttataa cgagaagtca aatgagtggg
     7681 taacgccaca aagggcttcc actgatactc cgacatgcaa caagtagttt atgcagacca
     7741 acgttgtgtg aagctcggtg aagtcggctg aaggataccg taaggtaaaa gaaatagtga
     7801 ataccacgaa agttctctag aggtaaaggg tgtatccgat aagtcgggtg tccaaaaaaa
     7861 cttaatatgg tgagtatgta caaacgtact gacgaactag cgaatgtact gtccgaaagg
     7921 catagctcct agaaatggga gagacagtga gcaaaaaatc actcattgtg ctaacttgtg
     7981 gttgagtaaa agtcgttgtt atgaaagacc atgctgcgtt acaggcgtag gagaagttag
     8041 ggggcaaaag ctagcatcta agttaaggca tgtggataga tataagggaa caggggaagc
     8101 catcaacatg gagagtgcat actcgatgaa gtggtagtaa gaaatatgtg aatgacaaat
     8161 aacttacttt agtgatggtg aaagtagagt cacgactgat gaaggttctg taatggaact
     8221 ggaggaacag gctcgattcg tctgtaaaag tcatgaaacc cgttcaacca tgggattctc
     8281 gtatgactaa aaaggtcacg agccaattgg agcgtgataa accatgacaa aagaaacaag
     8341 tccaaaagat aggaagttgc ggcatgcaga atattatggc atgacggaaa tcttcgataa
     8401 gatatactcc aaaagtttga agggagataa tttcaagaat ctaatgcaga ttatcatttc
     8461 tgataacaac attgtcctag catatcgtaa tatcaagaga aatcacggta gtgtgacaag
     8521 aggagaagat ggagtaacga ttaaagatat tgaaaatctc aaccgtaatg aattcgtaga
     8581 aattgttagg aagcgattcg cacattataa ccctcggaaa gtaaggcgtg tagaaattcc
     8641 taaatataat ggtaaattaa gaccgttggg tattccatca atatgggata gaatcgcgca
     8701 gcaatgcatc ttacaagtat tagaaccaat atgtgaagca aaattcaata atcacagcta
     8761 tggtttccgc ccaaatagat cagcagaaca cgcaatagca gattgtatgg cccgaatgaa
     8821 tcaaagtcac atggaatatg tggtagatgt agatatcaaa ggtttcttcg acgaagtcaa
     8881 tcatactaaa ctcatgagac aaatctggtc tatgggtata cgagacaaac agcttctctt
     8941 aatcattagg aaaatgttaa gagcacccgt agtacttcca aatgggaagt tgatataccc
     9001 aacaaaagga acaccacaag gcggaatact tagcccatta cttgcgaaca tcaatctcaa
     9061 cgaatttgac tggtggatag caaatcagtg ggaggaaaaa gatgccaaag agctaaaaca
     9121 tcattttagg aaaaatggag cttgggataa aggtaatatc tatcacaagc tcagaaagag
     9181 tacaactcta aaagaaatat acctagtgag atacgcagat gattttaaaa ttttctgtag
     9241 aaattataat gaagcgaaaa aagttttcta tgcaactaaa atgtggttac aggagcgtct
     9301 aaagttacca atatctgaag aaaagtcaaa aatcacaaat ctttcaaaag aagaaagtga
     9361 atttttaggc tttacgatga aattagtgac caaaggtaac aaaaaggttg ttaatacgca
     9421 tataggtaca aaagcacaaa gcatgattaa agtgaaacta aaaaaacaaa tcaaacgaat
     9481 tcaaaaaagt cctaatagca aagatacctt tatctatatt ggagtctata acagtatggt
     9541 tattgggatt cataattact tcaaaattgc gactcattgt agtgtagcaa tgcgaaaaat
     9601 tgcaagagaa ctcagaccaa gttttgaaat acgactacga gaacaaggaa tctcaaaaga
     9661 aggacaatac ggaaagtacg aaggatacaa gccttaccta cggtcaggac aaatacgata
     9721 tataagaggg tttccaatat taccagtagg gtatattcaa cacagacatc cgatgtgtaa
     9781 aaaaagaaat attaataaat acaccgaaga aggtagggaa gccattcata agaagcaaca
     9841 aaccgtagaa gaatggaaga tatcgtggtt gtgtagcaat ccagtgctta gtaagagagc
     9901 aacgattgaa tttgcggaca atcgaatttc aaaattcatc gctcaaaagg gaagatgtgc
     9961 agtaacaggt gaggagctta tcttatctga aatgcattgt catcatatta tcccttatca
    10021 tgaaagcaaa agtgatagct atgaaaacct agtgattgtt actgaggagg ttcatcgggt
    10081 aatacacgct acacaaagtg aaacgattga ggaacttcta aaatatctaa aattaaatcc
    10141 aaaacaaaaa gaaaaactca atgagttgag attaaaagtt ggtaacgaag aaatatctta
    10201 ataaatattt gtaggacggg aaaacagaat gtcttttata gacgagggaa cgccgtatgc
    10261 ggtgaaagtc gcacgtacgg tgtggactgg gggaaaagac ggagataata tcaaactctt
    10321 acctatcagt atcgattaat ccagatttta ttgaaacggt aaattatgtc acaaactttc
    10381 aacgagaaat gcgtaaagta gaagcaatga ctgttttagc tacacaatcc ccacaagaaa
    10441 tggtgccaga aaatatgtct tctgataact tctcgaaact gaagaaagtc tttgaatttt
    10501 cttctacgaa gatctttatg cgtatggatg aatctatttt acgtcatata gaaggattag
    10561 tgggtaaatc aatgacgaac tcagaattag aatccattcc tcaacaaaat caaggagacg
    10621 ctgtgatcaa ttttggctca aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc
    10681 aattaaaatt gtttgatgga ggaaaataat ggaaaaccaa aatgaaagtt taatcaagca
    10741 gtatgttaag cgtcgtgcaa agcggcgctt atttttgtgg ttgtttggta cttctgcagg
    10801 tttgattact attttgatca ctgtgttcgt gaccctattt ctaatacttg cggcgggctc
    10861 gattgataat tcggattcag attcaagcag tggtggcgaa gcttttacag gagaatattc
    10921 ggaaggctta ccgatttata aagaaatcaa aggaagaggc cctttttctg atgagattgc
    10981 gcaatatgcc gtaggtgcag cagtgaaata taaattatta ccaagtgtga tcctttcgca
    11041 atacggttac gaatcagcgt ttggtacatc ggcgtccgca agaaatgatc tcaactattt
    11101 tgggattact tggtttgatg gttgtctgtt tcctaaagga actgcacgag gaattggtgg
    11161 cattgaaggt ggctggtaca tgaagtttcc caactcaaaa gctgcatttt cctattacgg
    11221 ctttatggta gccacacaat ccaattttaa tgcttgtgta ggaaataaaa gccctggcgc
    11281 aagcttattg atcttaggac gaggcggtta tgccgctgca ggaattacgg aagatagtcc
    11341 gtattataca ggttgtatgt caattatcac ttctaataaa ttaaccgaat atgatgaatt
    11401 cgccatcaaa cattggggtg aaggtggcaa taacaacggc acgattactg gtgaatggac
    11461 gaatccattc cctggcagca gtttagataa aagttccttc agcggtggtc aattatttgg
    11521 aacgaatcca ggcggtgaat tccgtccgaa cggcttccat gatggattag attttggttc
    11581 agttgatcat ccaggaagtg agattcatgc ggttcatggc ggaaaagtcg tgtacgttgg
    11641 caatcctggt atttcaggtt tgggcgcgtg tgtgatcgtg attaattacg atggcttgaa
    11701 tatggtgtat caagaatttg caaatagtac aggaaattct cgagtgaaag taggcgatca
    11761 agtgaaagtt ggtcaagtaa ttgggatacg agatacagca cacttgcatt taggatttac
    11821 tcgaatggat tggcgtcaag cacaaggaca tgcattcatt gatgatggta catggatcga
    11881 tccgttacca tttttgaata gcagtaaaaa atagatcagg agaaaaaata tggaaaataa
    11941 aacgttaaaa atcgtcttaa cagctgcaat tagcgttagt ttaatcggca atattgcgtt
    12001 gttttggcag ctgaaagagg aacaagcagc aaatacctat aaaattgaag aagtggctaa
    12061 aaacgcgtct gagaagctca aaaaaaattc ctctgaggca caggcgaaga tcgacaagtt
    12121 aaatcaaacc atcaatgaga aaaaccaaga aatcgatcag ctgaagcaac aagatggcac
    12181 aaaagcaaaa gaggaagtaa ctgaagcaca aagaaagacg gcagaagagt tcggtcaatt
    12241 ggccattgat aaatcattaa gcagaaatga attaacggaa aaactaaaag atgtcgcaac
    12301 ccaagaagtg atcgacaaat taagtcctac agacgatgat caccaacatg atgattacgg
    12361 aagttactcc tttacattag gtgacgtgca aacttacgct caaacaagca gtgtaaaaga
    12421 ggaacaaaat tttgtcgtat ttgttgatta tacaatcggc aatccgcaat tcaaagatgt
    12481 caaaccacaa aaaataaaag gtggattaac tgtcactgaa aaacaagtag atggtcaatg
    12541 gaaagtcata gactttagtt atttcactcg ttaggaggaa gaaaatgagt aagaaaaaag
    12601 gagaaagaca acgtttcaaa aaacgaaaaa gttatcttag atggctggga atctttatga
    12661 ttctcggctt tttgattctt gctagtccga tttatacgaa tcagaaggta gatataccag
    12721 atgaggtaag cacgaacgcc tttttaaaca cgaaagagct gacaatggtt tcaaaagaaa
    12781 aaggagcgaa ccagcaatta gtttttgaat ttgcgatcga tgatgaagat caaagcttgt
    12841 taaaagaact ggctaatctt aattatcgcg tagaagtaaa gacagctaaa ggggattatc
    12901 aagcaatacg agccaaagtg attaaggtgt ctgatgatta ttttgcagtg aagctaacgc
    12961 atgtaccaga agaatatatc gccatgcgtt taaccattat tcctgaaaaa attgatccaa
    13021 aagtggatat gcaagaacca caagatctga tttattacgt gcacgaagat aaggttaaag
    13081 atcaagtgaa agatgaagat tatgaacaac acgcgatcaa ttacaaagtg aaaggataca
    13141 agaaagaaca aaaagcagct caaaaacaaa tggaatcttt ccaagcaacc attgatttga
    13201 atgaagaatt agtgaagaag ctaaaagatc aattgcctta tcaagtcgca gatgatcagg
    13261 aaaatactga aaacaagatt aatagctacc agcaagaaat tgaaacatcg aaaacgcaac
    13321 tgaaagatca agaagagatc attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag
    13381 aaaagattta gaaggacagg tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag
    13441 atactttcgc tgagcagaaa gtattgatta aagcctatct caaagttgta aaagctaaag
    13501 aaatatgtcg agaagtgaat ttatctaaca caacaatatc tgagttgaag aatggtcgaa
    13561 agaatttaga tactttaaaa ttcagatcat ttgaagctct atatgtagct gctaaaaaat
    13621 attttacagc tgataacaaa taaaaaacag gagtatttta tgtgaaaaaa gtatataaaa
    13681 aatgcttatg aatcagctat atcaccttaa ccatttgtta atgagatggg agaaaacatt
    13741 aaccacgatt ctaacgcaaa catcaagcac ataaaacatt taacactcct cctttctggc
    13801 tgaagatttc aaccaaacag gaagacagcg tatcaattat agcatacact ttttcttaaa
    13861 ttcatttttc atatgacaaa atatttcctt ctaatcgaca aagttagaag gattttttat
    13921 tgtctaaata cacaaataac cttaattttt aaagagcata aggaaacgtg atatcacttt
    13981 ctcacgaaag gtgttaggta ggctgtgcct accacgtttc taagaaagat aaatgatatc
    14041 aaaattttgg atttgtgtgt agatttgtgg gtaggatgaa agcgaatgtg ggcagtaaaa
    14101 taagcgtttg tgtgtagcaa aatcaaaaaa tgtgtaggga tatgtgtagc tacacatatc
    14161 cctacacata ataaatttgg aggaaaatat ggacttatta attagaaata ttgaagaaaa
    14221 gtatattaat aaaattgaca aacgttgtga agaacttacg gtcaagactg gcaaaaaatg
    14281 gagtcgaaat caatacctaa aagttttgat cgaaaatgat tttgatcacg ctcttctgaa
    14341 ttataaaaaa gaccaatttg atcggttgtt agaaaaattt gtggatattc aaacttataa
    14401 tacgaaaact ttagaagaat atattgcaac aaataatcaa ttaattggtt tgttaattga
    14461 ataataaaaa tagtattgtg aatttataaa aggaggaaat caattttggc aaaagcaaca
    14521 agaaaacatg tatatcttta cgatatgcat atcgaagtac ttgatgaaat aatttctact
    14581 catgaagata tatctaatta tagtgaagca gtcagacatt tgtgtatgat gtatgatcca
    14641 aaaatcgaag aaaaaataaa tccaattcaa attaaattga atgcaatggg aaaagaaatt
    14701 tctattttaa ccgaaatggt ttcagagttt gcggatcttc atttgaaaga tacaggaata
    14761 ctaattggta caaattcaac tgtttatcaa gaagcagaag aacgtgtgga aggaaaaatt
    14821 aaatccaatc aaaccaaaaa atattcttct aagttaaaga aaccagtgaa tgaatctgtt
    14881 cataccagag tagatccatt tgatccaacg aagattctaa aaagataaaa gtagaaggag
    14941 aacgtgatga ataaatcgcc tgcagttatt cttacttgtc aatttacctt gcccaataaa
    15001 aaatcttttt caacatacat tgattatatg acaagagaaa aagcactgga agaaatggaa
    15061 cgacgtacgc ccgaagaaga acaggaactg caacgaatca agaatgcttt agaggatttt
    15121 tatattcctg aaggagaaac tttttcggaa agtaagacgg
//
LOCUS       DASHFF010000061        14119 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.061, whole genome shotgun sequence.
ACCESSION   DASHFF010000061 DASHFF010000000
VERSION     DASHFF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.061"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..556)
                     /locus_tag="U2I75_002141"
     CDS             complement(<1..556)
                     /locus_tag="U2I75_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEN1697242.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="U2I75_002142"
     CDS             complement(572..1051)
                     /locus_tag="U2I75_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN1697243.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="U2I75_002143"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="U2I75_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="U2I75_002144"
     CDS             complement(2098..3204)
                     /locus_tag="U2I75_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1697244.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="U2I75_002145"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="U2I75_002145"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN1697245.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="U2I75_002146"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="U2I75_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN1697246.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="U2I75_002147"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="U2I75_002147"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN1697247.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="U2I75_002148"
     CDS             8175..8873
                     /locus_tag="U2I75_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1697248.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="U2I75_002149"
     CDS             complement(9041..11680)
                     /locus_tag="U2I75_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1697249.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="U2I75_002150"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="U2I75_002150"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN1697250.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..>14119
                     /locus_tag="U2I75_002151"
     CDS             13523..>14119
                     /locus_tag="U2I75_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1697251.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2544 g         4520 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagcg
//
LOCUS       DASHFF010000062        13945 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.062, whole genome shotgun sequence.
ACCESSION   DASHFF010000062 DASHFF010000000
VERSION     DASHFF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.062"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            137..310
                     /locus_tag="U2I75_002152"
     CDS             137..310
                     /locus_tag="U2I75_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697252.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            950..1423
                     /locus_tag="U2I75_002153"
     CDS             950..1423
                     /locus_tag="U2I75_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEN1697253.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="U2I75_002154"
     CDS             1432..1659
                     /locus_tag="U2I75_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1697254.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="U2I75_002155"
     CDS             2295..2666
                     /locus_tag="U2I75_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697255.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="U2I75_002156"
     CDS             complement(2922..3164)
                     /locus_tag="U2I75_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1697256.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="U2I75_002157"
     CDS             complement(3196..4098)
                     /locus_tag="U2I75_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1697257.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="U2I75_002158"
     CDS             complement(4111..4299)
                     /locus_tag="U2I75_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN1697258.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="U2I75_002159"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="U2I75_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN1697259.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="U2I75_002160"
     CDS             complement(4914..5807)
                     /locus_tag="U2I75_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1697260.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6808)
                     /locus_tag="U2I75_002161"
     CDS             complement(5885..6808)
                     /locus_tag="U2I75_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1697261.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPIYFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6857..7207)
                     /locus_tag="U2I75_002162"
     CDS             complement(6857..7207)
                     /locus_tag="U2I75_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1697262.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7240..8142)
                     /locus_tag="U2I75_002163"
     CDS             complement(7240..8142)
                     /locus_tag="U2I75_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN1697263.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8135..8992)
                     /locus_tag="U2I75_002164"
     CDS             complement(8135..8992)
                     /locus_tag="U2I75_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN1697264.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9326..10135
                     /locus_tag="U2I75_002165"
     CDS             9326..10135
                     /locus_tag="U2I75_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1697265.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10175..10672
                     /locus_tag="U2I75_002166"
     CDS             10175..10672
                     /locus_tag="U2I75_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1697266.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11057..11130)
                     /locus_tag="U2I75_002167"
     tRNA            complement(11057..11130)
                     /locus_tag="U2I75_002167"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11094..11096),aa:Ile,seq:cat)
     gene            11317..11640
                     /locus_tag="U2I75_002168"
     CDS             11317..11640
                     /locus_tag="U2I75_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697267.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11804..12058
                     /locus_tag="U2I75_002169"
     CDS             11804..12058
                     /locus_tag="U2I75_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697268.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12128..12373
                     /locus_tag="U2I75_002170"
     CDS             12128..12373
                     /locus_tag="U2I75_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1697269.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12449..12814
                     /locus_tag="U2I75_002171"
     CDS             12449..12814
                     /locus_tag="U2I75_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697270.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12875..13438)
                     /locus_tag="U2I75_002172"
     CDS             complement(12875..13438)
                     /locus_tag="U2I75_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697271.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4088 a         2577 c         2303 g         4977 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc atctttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaatatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
     6601 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
     6661 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
     6721 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
     6781 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
     6841 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
     6901 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
     6961 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
     7021 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
     7081 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
     7141 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
     7201 tttccattcc tactccccct cttcagttgt tgtcttctat tatttaacaa ctacatctgt
     7261 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
     7321 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
     7381 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
     7441 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
     7501 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
     7561 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
     7621 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
     7681 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
     7741 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
     7801 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
     7861 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
     7921 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
     7981 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
     8041 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
     8101 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
     8161 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
     8221 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
     8281 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
     8341 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
     8401 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
     8461 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
     8521 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
     8581 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
     8641 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
     8701 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
     8761 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
     8821 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
     8881 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
     8941 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
     9001 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
     9061 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
     9121 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
     9181 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
     9241 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
     9301 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
     9361 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
     9421 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
     9481 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
     9541 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
     9601 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
     9661 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
     9721 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
     9781 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
     9841 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
     9901 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
     9961 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    10021 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    10081 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
    10141 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
    10201 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
    10261 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
    10321 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
    10381 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
    10441 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
    10501 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
    10561 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
    10621 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
    10681 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
    10741 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
    10801 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
    10861 caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt
    10921 tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca
    10981 agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    11041 ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
    11101 gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt ttgttacttt
    11161 tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa aataatattg
    11221 ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt
    11281 attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca
    11341 tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat
    11401 caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt
    11461 cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt
    11521 gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc
    11581 gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag
    11641 ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca
    11701 tttttcattg atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata
    11761 acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag
    11821 agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg
    11881 gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa
    11941 agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact
    12001 ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt
    12061 actattgatt gcatgtgtag ctttctatag tcattataac tattctacta taatgaaagg
    12121 tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc gtctgaattt
    12181 cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa gtattccttt
    12241 attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg aattaatcta
    12301 tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag aaacagcaag
    12361 caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg tctaaggtta
    12421 acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat tgatttcaaa
    12481 gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa gaggaaatgg
    12541 ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt atagattatt
    12601 ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc cagcaccaga
    12661 gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt tacctactgt
    12721 agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag aatgtttagc
    12781 aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc ttctatctcc
    12841 tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg taagttttag
    12901 ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt tcatcgccaa
    12961 cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca tcttcgacgt
    13021 ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt tttctgttgc
    13081 ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg cctaatattc
    13141 ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat tttaactcct
    13201 ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag tcagaacaac
    13261 ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcata aggttccccc
    13321 cctgaaaata gactaaaata ataacgcttt ctttttttgt tttcagacga aaattaataa
    13381 tttctaaata ttctttaaaa aagcttataa ctattatgcc ttttttaata tataacacag
    13441 ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata
    13501 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
    13561 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
    13621 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
    13681 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
    13741 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
    13801 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
    13861 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
    13921 attcagccca ttcaacgacc aatga
//
LOCUS       DASHFF010000063        13852 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.063, whole genome shotgun sequence.
ACCESSION   DASHFF010000063 DASHFF010000000
VERSION     DASHFF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.063"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..902)
                     /locus_tag="U2I75_002173"
     CDS             complement(<1..902)
                     /locus_tag="U2I75_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEN1697272.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="U2I75_002174"
     CDS             1185..2153
                     /locus_tag="U2I75_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1697273.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="U2I75_002175"
     CDS             2266..2544
                     /locus_tag="U2I75_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1697274.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="U2I75_002176"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="U2I75_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN1697275.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="U2I75_002177"
     CDS             3001..3756
                     /locus_tag="U2I75_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1697276.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="U2I75_002178"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="U2I75_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEN1697277.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="U2I75_002179"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="U2I75_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEN1697278.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="U2I75_002180"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="U2I75_002180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN1697279.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="U2I75_002181"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="U2I75_002181"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN1697280.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="U2I75_002182"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="U2I75_002182"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN1697281.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="U2I75_002183"
     CDS             8493..9035
                     /locus_tag="U2I75_002183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN1697282.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="U2I75_002184"
     CDS             9058..10614
                     /locus_tag="U2I75_002184"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN1697283.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="U2I75_002185"
     CDS             10628..11530
                     /locus_tag="U2I75_002185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN1697284.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="U2I75_002186"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="U2I75_002186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN1697285.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="U2I75_002187"
     CDS             12973..13395
                     /locus_tag="U2I75_002187"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN1697286.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4575 a         2474 c         3013 g         3790 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag acttttgtgg ctctttgtca ataaggactg atgagttgtg
    13741 caaaatcaaa tctgagtcag aatgaaccac attctggctc agattttttg ttatcttaca
    13801 ttgattaatt gattgatcaa aaagattcta attctcatgt tctcaaatga tt
//
LOCUS       DASHFF010000064        13817 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.064, whole genome shotgun sequence.
ACCESSION   DASHFF010000064 DASHFF010000000
VERSION     DASHFF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.064"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="U2I75_002188"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="U2I75_002188"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN1697287.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="U2I75_002189"
     CDS             complement(2261..2512)
                     /locus_tag="U2I75_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN1697288.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="U2I75_002190"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="U2I75_002190"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN1697289.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="U2I75_002191"
     CDS             3462..3680
                     /locus_tag="U2I75_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697290.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="U2I75_002192"
     CDS             complement(3969..4481)
                     /locus_tag="U2I75_002192"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN1697291.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="U2I75_002193"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="U2I75_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN1697292.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="U2I75_002194"
     CDS             complement(6934..7377)
                     /locus_tag="U2I75_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN1697293.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="U2I75_002195"
     CDS             complement(7378..8166)
                     /locus_tag="U2I75_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1697294.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="U2I75_002196"
     CDS             complement(8163..9104)
                     /locus_tag="U2I75_002196"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN1697295.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="U2I75_002197"
     CDS             complement(9281..9868)
                     /locus_tag="U2I75_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN1697296.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="U2I75_002198"
     CDS             complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="U2I75_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN1697297.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11699..12709
                     /gene="gap"
                     /locus_tag="U2I75_002199"
     CDS             11699..12709
                     /gene="gap"
                     /locus_tag="U2I75_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1697298.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12794..13729)
                     /locus_tag="U2I75_002200"
     CDS             complement(12794..13729)
                     /locus_tag="U2I75_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1697299.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3811 a         3095 c         2436 g         4475 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaaga gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgtaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgtattaa ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat
    11701 gacagtaaaa gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat
    11761 caaagaagtc tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat
    11821 gttagcacaa ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc
    11881 aacagaagac tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc
    11941 aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt
    12001 ttatacatct gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat
    12061 ttctgcccca gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga
    12121 tgaaaacgac aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc
    12181 ttatttctta aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta
    12241 cacttccaca caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg
    12301 ttcagctgct gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt
    12361 tatccctgag ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg
    12421 ttcattaact gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga
    12481 agcaatcaaa aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt
    12541 ttctagcgat gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt
    12601 aacaactgct ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg
    12661 atttacttgc caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc
    12721 aatcgataaa agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta
    12781 cttttaaact cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt
    12841 tttcaggatc tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt
    12901 ttggtccctt cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt
    12961 cgtctccatg tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg
    13021 cttcaccgaa aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg
    13081 gaaatgctgg aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag
    13141 aataaccgca atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca
    13201 tcataactgt tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat
    13261 tgttttctag cactttgttt atccactgta catagtcttt acgctctggc catccaaatc
    13321 caatgtaatc cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac
    13381 ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat
    13441 gagccacaag agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt
    13501 tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt
    13561 tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt
    13621 ctttctcaga aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa
    13681 caatcagagc taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat
    13741 ctcatttctc ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca
    13801 atgttcattt ttataac
//
LOCUS       DASHFF010000065        13776 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.065, whole genome shotgun sequence.
ACCESSION   DASHFF010000065 DASHFF010000000
VERSION     DASHFF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.065"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="U2I75_002201"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="U2I75_002201"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN1697300.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="U2I75_002202"
     CDS             complement(1172..1867)
                     /locus_tag="U2I75_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN1697301.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="U2I75_002203"
     CDS             complement(1880..2596)
                     /locus_tag="U2I75_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN1697302.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="U2I75_002204"
     CDS             2926..3531
                     /locus_tag="U2I75_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN1697303.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="U2I75_002205"
     CDS             3856..4179
                     /locus_tag="U2I75_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN1697304.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="U2I75_002206"
     CDS             4361..5731
                     /locus_tag="U2I75_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN1697305.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="U2I75_002207"
     CDS             complement(5861..7162)
                     /locus_tag="U2I75_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1697306.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9350)
                     /locus_tag="U2I75_002208"
     CDS             complement(7392..9350)
                     /locus_tag="U2I75_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN1697307.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="U2I75_002209"
     CDS             complement(9527..9793)
                     /locus_tag="U2I75_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1697308.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="U2I75_002210"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="U2I75_002210"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN1697309.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="U2I75_002211"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="U2I75_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN1697310.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="U2I75_002212"
     CDS             13137..13700
                     /locus_tag="U2I75_002212"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1697311.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
BASE COUNT         3946 a         2879 c         2544 g         4407 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttc
//
LOCUS       DASHFF010000066        13703 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.066, whole genome shotgun sequence.
ACCESSION   DASHFF010000066 DASHFF010000000
VERSION     DASHFF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.066"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            6..245
                     /locus_tag="U2I75_002213"
     CDS             6..245
                     /locus_tag="U2I75_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697312.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="U2I75_002214"
     CDS             312..521
                     /locus_tag="U2I75_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697313.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="U2I75_002215"
     CDS             581..1252
                     /locus_tag="U2I75_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN1697314.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="U2I75_002216"
     CDS             1305..3281
                     /locus_tag="U2I75_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1697315.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="U2I75_002217"
     CDS             3294..4049
                     /locus_tag="U2I75_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1697316.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="U2I75_002218"
     CDS             4046..4870
                     /locus_tag="U2I75_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697317.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5171..7270
                     /locus_tag="U2I75_002219"
     CDS             5171..7270
                     /locus_tag="U2I75_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1697318.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="U2I75_002220"
     CDS             complement(7308..9365)
                     /locus_tag="U2I75_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEN1697319.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="U2I75_002221"
     CDS             complement(9479..9691)
                     /locus_tag="U2I75_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697320.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="U2I75_002222"
     CDS             9878..10345
                     /locus_tag="U2I75_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697321.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="U2I75_002223"
     CDS             10342..10584
                     /locus_tag="U2I75_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEN1697322.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="U2I75_002224"
     CDS             10602..10904
                     /locus_tag="U2I75_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697323.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="U2I75_002225"
     CDS             10976..13030
                     /locus_tag="U2I75_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697324.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13030..>13703
                     /locus_tag="U2I75_002226"
     CDS             13030..>13703
                     /locus_tag="U2I75_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HEN1697325.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVV"
BASE COUNT         4900 a         2123 c         2744 g         3936 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc tgctgttgcg gcagtggctt
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactttta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactccct caggagaaga gctaccagaa
    13141 aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata cctcccttta
    13201 gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt taagggaagc
    13261 ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca ttttgattca
    13321 gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt agcaatttcg
    13381 acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgt
//
LOCUS       DASHFF010000067        13346 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.067, whole genome shotgun sequence.
ACCESSION   DASHFF010000067 DASHFF010000000
VERSION     DASHFF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.067"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(45..1364)
                     /locus_tag="U2I75_002227"
     CDS             complement(45..1364)
                     /locus_tag="U2I75_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1697326.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="U2I75_002228"
     CDS             complement(1361..2014)
                     /locus_tag="U2I75_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1697327.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="U2I75_002229"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="U2I75_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="U2I75_002230"
     CDS             complement(2724..3440)
                     /locus_tag="U2I75_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEN1697328.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="U2I75_002231"
     CDS             complement(3564..3749)
                     /locus_tag="U2I75_002231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697329.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="U2I75_002232"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="U2I75_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="U2I75_002233"
     CDS             complement(5356..6387)
                     /locus_tag="U2I75_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1697330.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="U2I75_002234"
     CDS             complement(6394..7224)
                     /locus_tag="U2I75_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1697331.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="U2I75_002235"
     CDS             complement(7221..8036)
                     /locus_tag="U2I75_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1697332.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="U2I75_002236"
     CDS             complement(8033..8857)
                     /locus_tag="U2I75_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEN1697333.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="U2I75_002237"
     CDS             complement(8847..9947)
                     /locus_tag="U2I75_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1697334.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="U2I75_002238"
     CDS             10125..10910
                     /locus_tag="U2I75_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEN1697335.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="U2I75_002239"
     CDS             10943..11326
                     /locus_tag="U2I75_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN1697336.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="U2I75_002240"
     CDS             11402..11533
                     /locus_tag="U2I75_002240"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1697337.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="U2I75_002241"
     CDS             11502..11738
                     /locus_tag="U2I75_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1697338.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="U2I75_002242"
     CDS             complement(11816..12787)
                     /locus_tag="U2I75_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEN1697339.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4055 a         2912 c         2306 g         4073 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttat
//
LOCUS       DASHFF010000068        13146 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.068, whole genome shotgun sequence.
ACCESSION   DASHFF010000068 DASHFF010000000
VERSION     DASHFF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.068"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(77..149)
                     /locus_tag="U2I75_002243"
     tRNA            complement(77..149)
                     /locus_tag="U2I75_002243"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="U2I75_002244"
     CDS             530..1099
                     /locus_tag="U2I75_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN1697340.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="U2I75_002245"
     CDS             1086..1946
                     /locus_tag="U2I75_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1697341.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="U2I75_002246"
     CDS             2136..2840
                     /locus_tag="U2I75_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1697342.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="U2I75_002247"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="U2I75_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN1697343.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="U2I75_002248"
     CDS             4677..5993
                     /locus_tag="U2I75_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697344.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="U2I75_002249"
     CDS             5994..6863
                     /locus_tag="U2I75_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN1697345.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="U2I75_002250"
     CDS             6918..7727
                     /locus_tag="U2I75_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1697346.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="U2I75_002251"
     CDS             complement(7830..8459)
                     /locus_tag="U2I75_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN1697347.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="U2I75_002252"
     CDS             8629..9921
                     /locus_tag="U2I75_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1697348.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="U2I75_002253"
     CDS             complement(9997..10659)
                     /locus_tag="U2I75_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1697349.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="U2I75_002254"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="U2I75_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN1697350.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="U2I75_002255"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="U2I75_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN1697351.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DASHFF010000069        13135 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.069, whole genome shotgun sequence.
ACCESSION   DASHFF010000069 DASHFF010000000
VERSION     DASHFF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.069"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..456
                     /locus_tag="U2I75_002256"
     CDS             <1..456
                     /locus_tag="U2I75_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697352.1"
                     /translation="AERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEH
                     KNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKS
                     YGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            517..1986
                     /locus_tag="U2I75_002257"
     CDS             517..1986
                     /locus_tag="U2I75_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN1697353.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1997..4201
                     /gene="topB"
                     /locus_tag="U2I75_002258"
     CDS             1997..4201
                     /gene="topB"
                     /locus_tag="U2I75_002258"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1697354.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            4217..4366
                     /locus_tag="U2I75_002259"
     CDS             4217..4366
                     /locus_tag="U2I75_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697355.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            4440..5033
                     /locus_tag="U2I75_002260"
     CDS             4440..5033
                     /locus_tag="U2I75_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN1697356.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            5049..5948
                     /locus_tag="U2I75_002261"
     CDS             5049..5948
                     /locus_tag="U2I75_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN1697357.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            5951..6436
                     /gene="ssb"
                     /locus_tag="U2I75_002262"
     CDS             5951..6436
                     /gene="ssb"
                     /locus_tag="U2I75_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1697358.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            6802..7062
                     /locus_tag="U2I75_002263"
     CDS             6802..7062
                     /locus_tag="U2I75_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697359.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            7503..7709
                     /locus_tag="U2I75_002264"
     CDS             7503..7709
                     /locus_tag="U2I75_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN1697360.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7709..7960
                     /locus_tag="U2I75_002265"
     CDS             7709..7960
                     /locus_tag="U2I75_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697361.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            7975..8412
                     /locus_tag="U2I75_002266"
     CDS             7975..8412
                     /locus_tag="U2I75_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697362.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            8405..9112
                     /locus_tag="U2I75_002267"
     CDS             8405..9112
                     /locus_tag="U2I75_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697363.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            9493..9672
                     /locus_tag="U2I75_002268"
     CDS             9493..9672
                     /locus_tag="U2I75_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697364.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            10013..10276
                     /locus_tag="U2I75_002269"
     CDS             10013..10276
                     /locus_tag="U2I75_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEN1697365.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            10270..10620
                     /locus_tag="U2I75_002270"
     CDS             10270..10620
                     /locus_tag="U2I75_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1697366.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(10644..11258)
                     /locus_tag="U2I75_002271"
     CDS             complement(10644..11258)
                     /locus_tag="U2I75_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1697367.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            11572..11994
                     /locus_tag="U2I75_002272"
     CDS             11572..11994
                     /locus_tag="U2I75_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697368.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            12004..12207
                     /locus_tag="U2I75_002273"
     CDS             12004..12207
                     /locus_tag="U2I75_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697369.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            12418..13002
                     /locus_tag="U2I75_002274"
     CDS             12418..13002
                     /locus_tag="U2I75_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1697370.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         4754 a         2142 c         2625 g         3614 t
ORIGIN      
        1 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
       61 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
      121 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
      181 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
      241 catttgaagg atgcggcgtt tattgaagat ccacagcata atcaagtaat tttaaaagga
      301 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
      361 tcattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
      421 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
      481 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
      541 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
      601 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
      661 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
      721 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
      781 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgagacagaa
      841 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
      901 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
      961 gctgaactaa agccgaaaca tgaacggcaa aattttttag ttggagacga ccgtttagat
     1021 catgcggttg cttttttatg gaaagatcca cagacaggag agacagtagg ggcaagttat
     1081 caaggaacga tcgtcgattt taatcgtttc ggtaaacggg gaacgtataa acacattgac
     1141 aagaatccta cgccaaatca cggatttaat ttaaaaatag gcgatccgaa gcatttaaaa
     1201 ttctttgaaa gtagtattga tctgttaagt tacgctgcat tgaatcggga gaaactacaa
     1261 gatgcctggt tagtttcgat ggacgggtta aaacatcatg taatcagtca ttacgttgaa
     1321 gagtcgattt ctgaattaag acgaaaacaa acatttcctc aatcgattga aatatgtgtg
     1381 gacaatgatc gtgcaggaca tattttttat gaaaaagaac agatgaaggg aatcgttgat
     1441 ccttttacca ataagaaaat ccgttgtgag cgaggaattc caaatgattg gcaagtacct
     1501 aaagaataca aagctactta tgaagcagtc gcaaaagaaa tgagtgttga acctgaagca
     1561 attatggcca tacacaaaac tgaaactaat ttacagctga caaatcaatt ggtgtctgct
     1621 catgatgttc aatctacgtt tggaaagatg ttggcgaagg gcgaaccagt agaaacaatt
     1681 gatttgaaag aagcatgtac cacggtcgcg aaggaactga aagtatgtga acgcgcagat
     1741 ggaacataca actttgatcg tttttatagt agaaaagcca acataaaaga cgttaatgca
     1801 ggaatccttc tttcctataa agcagaacaa tactacaaag gctataaaaa gcatgaacat
     1861 gaatttgtac ctgaagtgaa aaaggattgg aatgatcaat tgaagcatga gatccagcaa
     1921 caagaaatca gaaagcaaaa acgtgcgatg ttgtttcaac aaggcagaca acaagaaagg
     1981 gagtgaagtc gtcactatga aaaccgtcat tttagcagaa aaaccgtcac aagcgaaggc
     2041 ttatgccgat agcttttcta aagctacccg aaaagatggg tattttgaga tccaagatcg
     2101 attgtttaca ggagaaactg tgatcacgta tggttttggt catttagtcg agttagattc
     2161 gcccgatatg tacgatgaaa aatggaaaca atgggcatta gaacatttgc ctattttccc
     2221 caatcaatac cactatcatg ttcctaaaga taagaaaaag cagtttagtg tcgtcaaacg
     2281 acagctgcag tcagctgata cgattgtgat cgcgactgat agcgatcgtg aaggggagct
     2341 gattgcctgg tcaatcattc aacaagcagg cgccaatcaa ggaaaaacct ttaaaagact
     2401 ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccaacagt tacgagacgc
     2461 agaagagacc tatccaaaat ttgaagaagt acaagcacgt cagatcgccg attggttaat
     2521 tgggatgaac ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag
     2581 tttttcttta ggtagagtcc aaaccccaac actctatatg atttatcaat tgcaggaaaa
     2641 gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gcccaagtaa tcgctcaaaa
     2701 tggcgcattt gatgcgaagc ttgatccaaa tgaaacccaa gcgacacaag aagcatttga
     2761 agcctattta aaagaaaaag gtgttcaatt aggaaaacag ccaggtacga tccttcaagt
     2821 cgaaacagag aaaaaaagtg cggctagccc acgtcttttt tctttatcga gccttcagtc
     2881 taaaatgaat caactcatga aagctagtgc caaagatact ttggaagcca tgcaagggtt
     2941 gtatgaagga aaatatctaa gctatccgcg aacggatacc ccttatatta cggaagggga
     3001 atatgcgtat ctgttggatc acttagacga atacaagcat tttttaaaag ccgaagcgat
     3061 tccgactcct attcatacac cgaatagtcg gtatgtgaat aataagaaag tccaggaaca
     3121 ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac aactaagtcc
     3181 actgcagcag gccatttatg aacaagtgtt aaaaacaacg gtggccatgt ttgcggaaaa
     3241 atatacctat gaagaaacaa cgatcttgac gcaagtccag cagcttcagt taaaggcaat
     3301 cggaaaagta ccgatagatc taggttggaa gaagttattt ggcaaagaga acgacggaaa
     3361 agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg tgacggtaga
     3421 tctacaagtc cttgaaaaag aaaccaagcc accacagcct tataccgaag gtacgttgat
     3481 cacggcgatg aaaacagctg ggaaaaccgt tgatagtgaa gaagcgcaat ccatattgaa
     3541 agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa ccttgaaaca
     3601 aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag ggattttgct
     3661 ttgccaagcc gtggaaaaag agccgctgct tacgagtgcc gaaatgaccg ccaaatggga
     3721 aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc tagccaatat
     3781 ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat ccactgattt
     3841 tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg cgcgtcattt
     3901 aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg tggcagcatg
     3961 tacgtctgaa gcctgtgatt tcaaactatg gacaacgatt gccaaaaaga aacttacagc
     4021 gacacagctg aaagagatca ttcaaaatag ccgaacaagc caaccagtga aagggttaaa
     4081 aggtcagaag ggttcgtttg aagcaacgat tgttttaaaa gaagacttca ctacaggctt
     4141 tgaattttct gaaaagaaaa aaacgaactt caagaaaaga acacgacgca ctacaaaata
     4201 atggagggtt aatcttatgc gtaatacaac acacaaacaa caagaaatcg aaaatcaaaa
     4261 agccaaaatt cgttcagaac aggaaaaaat gcgtgccagc aaacgaagac tgcagcaatt
     4321 gaagaaccag cagcggattc aaaaagaaat cgaattagat cgataaattc ctttcttgtt
     4381 aatataaaac taacatgtta tagttgtgtt agttttatat taactttagg ggaagagtaa
     4441 tgaaagacaa aatccaatgt atttttgata aacataatgg caacctatcc ttgaaggatg
     4501 ctagaagtga aggattatcg cctatgacgt tagatcgatt agccgaaaag gataaaatag
     4561 aaaaggtagc accaggtttc tataccttaa aggatgcgtt cgtggatgaa ttgtatctcg
     4621 ctcagagtat ctattccaga gggatttttt ctcatgagac tgcgctcgat ctttttcaag
     4681 taggaacata tatccctaaa aagatcaata tgatgtttcc taaaggatac aatgtatcaa
     4741 aggagaaatt agatcagtat gccattcaac cgcattacac taataaagaa aattttgacc
     4801 tgggagtcac agaaacagaa agcttttacg gcaataaaat tttagtctat gatttagagc
     4861 gaacgttatg tgacatgtgg aatccaagat acaaagcaag cgtagaggta aaacaagaag
     4921 cattaaaaga atatatgaca agtaccagta gaaatacaaa taagctaaga agatacatgg
     4981 atattttaaa atcaccaaaa gaaatgactt tgtatatgtt gccactttat tgagaggagt
     5041 aaagatgaat gacaccgaca caatttaaag agaaaacaag aaatttcgca gcagagaaac
     5101 atttaaaccc acaaattgtc caaagatatt atatgatgga aaaattatta gaatgtattg
     5161 ttgaatcgcc ttatcatgat gattttgtat taaaaggggg atttctgatt ggatcaaaat
     5221 acgggttaga aaaccgttca accgttgata ttgatacgac ttatcgaaat tcgaaactaa
     5281 caaaagaaaa gttagaaact gttttttctg aattaacagc taatcctaca aaagatggaa
     5341 ttcgttttga attaaaggga ttaacagaga cgagggaagc cgattattat ccaggttttc
     5401 aagcaaagtt tattgcttat cttgaaaatg cacgtgtacc ttttaaatta gatattactt
     5461 caggtgattc tattattcca gcagcattga tgtatgaaca caaattaatg tttgaagata
     5521 aaacgattca tattccagct tatcctacgg aacaaattct agctgaaaaa ttacatgcca
     5581 cgtttagttt tggaaaagat aattcgcgca tgaaagattt ttatgacctg tatatgattc
     5641 cgaaattaga acgtatcgat gagaaaaatt tgtataagag tgttcaagga acattcaagg
     5701 aaagaaataa tcaatcggtg tttaagcatt tgtatgatca ggaaatgcct caattggtga
     5761 aagatgaggg cttgagagaa aagtggaaaa gataccaaca agaaaactat tttgccaaag
     5821 acatttcttt cgaatctaca ctaacttccg ttgattccct gatgcaatca attgtagagc
     5881 aagagaaggc agaacgaaaa caaaatttta agagatcaca acaaatgaat caagaaatgg
     5941 agagataaag atgatcaata atgtaacttt agtagggcga ttaactaaag atccagattt
     6001 acgttatacc gcaagtggca cagcagtggc gactttttcc ctagccgtta atcgtaattt
     6061 cacgaatcaa aacggcaatc gagaagctga ttttatcaat tgcgtgattt ggcgcaaacc
     6121 agcagaaacg atggcgacat tagctaaaaa aggcagcttg atcggcgttg tcggacgaat
     6181 tcaaacacgg acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc
     6241 cgataacttc caactattag aaagtaaagc agcgacagaa agccgtatgc acgctgatca
     6301 atcgtccagc agtccaagcc ctaccacctt tgaacaacgg gatacagcga cgcctaacaa
     6361 caatggattg aacgcttcac agaatccatt tggtggacaa tcaatcgata ttagcgatga
     6421 tgatctaccg ttctaagtag cattttctat tggttgcatt tgctacggtt tatgttataa
     6481 tttagacaac aaaaagaatt ttcaatctat aaccaaaaac cactagctgg cgggctagtg
     6541 gctttctttt acgtttctat tataagaatt aacgggtagg cttactgtct ttcttggcaa
     6601 atttacaatc aagccatttg cagatatagt gcgcggctac ttctgccatg attgcaaaaa
     6661 caaaatcaaa aagaattttc aaacctataa cctcctcccc ttgccagatt aggccggggt
     6721 agcagacagc aagactatta tagcataatt gaaatatcca aatgattgga tttttgctat
     6781 aattttagcg aggtgattgt gatgaacaaa tatgttggtt tgcttgataa aatccgagta
     6841 ataaaaacgc aaccgttatt ggttcgcttt actttacaaa cgattcatga atcgattaat
     6901 tgtgtagttg cagatattga aattatcgat aaattgctaa ttatggatga tggaaaatac
     6961 aatattgccg tcacaggtca cttcaataaa cgaaatcagc tagttattgc tagcatgtat
     7021 gttagaaatc ccgatcattt tacgcgatcg atgggcatat aaataaaaga agagttgtag
     7081 tcctaaatga ggattgcagc tctttttgtt ttacatagaa acaggaaaag acctttttag
     7141 gaagacacaa gtgccttctc aattgaaaaa cctttctctc actccaaccg ccaccgccgt
     7201 tcgagaagaa aggaagtgaa cgctgcacgt tcactaagat atggtgtctt gcaaagcaag
     7261 acaaaataga caaaacttcc tctcaccaaa aggttcgttc ctgttcgtta tctctttatt
     7321 gatttgccag accggcaaca agttgccttt tatcaccaaa aagcgacaag gacaagggta
     7381 aatggcacgc tgcgcgccct tgtccttgtc gcttttcgtt gatttattct ggccaacaaa
     7441 tcaataaaga aagaactaac gaaaggaagt aaaacctttc ctaagaggag gcagaatcaa
     7501 acatgcgaaa gaaatttgat tattggggtg ttcccttcag cgaattattc ccaaactatc
     7561 atgcaccaca tactgttgaa tgcgattgtg gagaaagagc gaaatgtatc aaaagctatc
     7621 gtttgtatca atgtccaaca tgcgggaaaa aatatacctt gtcttatggc gattacattt
     7681 tgattgatga gaaaggaaag aaacgctgat gaaaacacaa cacaaattat tgactgtaaa
     7741 agaagtcgtt caaattttgg tggacaaaga agtcatttct gaaaaagtag cagaagcttt
     7801 acttattccg cggtactgga cgatagaaac ccctttagta gaggatgcac cgactatgct
     7861 atttgaagac ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa acatggaaac
     7921 ttatgaaatc gaatattggt taaccaaatt agagaactaa aaaggagaaa aaatatggct
     7981 caaaattact atgacgaatt tgtcaaatta cctttggata aaatggcaca aaaaatggag
     8041 gatatgacgt tcctatacca tgaaacacgt gtacccaaaa aacattacaa ggaaaaattg
     8101 tcagtcgcag tggaagaaat gattgaatca ggtgtcgaaa tgaatctcat tgcgacgtac
     8161 tatcgaacac tggaagaact gaaaaaacaa aatggaaaat ggtttttcca agccttacta
     8221 tgcttggaag taggtgtcaa accaagcact atcaaacctt ccgaatatca agccttagaa
     8281 ctgacttatg caaaattcgc acagattaag aaagctaaag caatggctag cgactggcta
     8341 gatacttttg aaagtatcga aaaaaatggt gcattttata cgattgaatc ggaggagaaa
     8401 gataatgaat aaggtggttt atcagctaac aacagaagat gtttcgcaat ttttaacaag
     8461 caaagaacac ttgaatgaag taatcagacg gattcaaaaa gactttagtt gtatggaaga
     8521 aatgaagtac tttattcaaa gagtggaaga acagttgtat atggaaagcg aaacaagtca
     8581 aacgaaggtc acagtcagcc ataatcattt agacgtgtat aaacgtcgtc ctgaaacggt
     8641 aaaaaaagga acagagcttc tacgactaga aacagaaaaa tacatcatga atgtgtacct
     8701 agaaaggagt gagctggtcg aaaactttgt cagctacacg atttgtagga aacactatta
     8761 ctcagaatca gaatattatt caattttcga gagcatggaa ttaatggaag aagaaatgga
     8821 agagctaaac agcaaaaatg cgacggtaga cgattattac cttgtattta ctaggttttt
     8881 gacgtgtggg acggaatcta tcgtgttaat gggaatggat tatgaactcg ctgtacccga
     8941 agcatttttg agggaatatg tagaagaagt agataaccca ggcgacgact ggtacacgac
     9001 tttaactgat tttttagaca cctacactta cgacgataca gaagaaatcc aaaatgaact
     9061 tgaccgtaaa cgaatgccat atcaacgatt tgaaaggaaa aaaggtaaat aaccatttat
     9121 accgaaaagt gaggatgtgt gaaataagaa caaaaaacct tgaaattaag ggaatggttc
     9181 ttatttcgca ccaatcgctc caaccgcccc cgccgcttag gtggtgcgaa ataagcgggc
     9241 aaagcccgat aacctaagag aaagaaggag atccatatga ataaaatcat tcctctattg
     9301 ttcgtactgt ttgttggcga gatacttatg cgtcatgctg cagctgtaca tttaaacttc
     9361 gtagtattct taggaatact ggtattttgt ttgatctttt ggcgatttgt attagccctt
     9421 gtgagtctac tattcaagat gattgcagcc atatttttca tagggttatt tcgatgaaag
     9481 gagagagtac taatgccaat tgcaatagga aataaacggt tacccgtgac attagatgaa
     9541 aagagacaaa aagaattgca gcaactaaag cagaagtacg gaaaaagtga atctaagatt
     9601 atgtgtattg cgttagatct attgatcgcg caagaaaaag caggatttga tataccagca
     9661 ctaaaaaagt gacgtcactt ttcatttgaa gaaacaaaag tgatagcact taattgttcc
     9721 tactatttag aatattttct atgtcctttt tattccattt tgtatcaata actatgaaat
     9781 ctttaaagta tccagaagag attattttta gatcaatttt tttcttacct gattctaaag
     9841 attttgtttt cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca
     9901 tagtttatgg attcatggaa tgttaatcta atttaataga aaaggataat taagtatata
     9961 cgttttttct tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat
    10021 gttacctata aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga
    10081 atatcttgga attcatacag aagacaaagt gaagataata caagaagaag taaacggtca
    10141 gaagcggcta attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt
    10201 tgaaaattac actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg
    10261 taatgaaaaa tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct
    10321 cgatctggac acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt
    10381 gcggattata gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg
    10441 ctatatgtaa atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat
    10501 caactaacga caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat
    10561 agtttagttg aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa
    10621 aatagaaaca gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta
    10681 tatacggttt gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt
    10741 tttagatcct caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca
    10801 ctgtatagct taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt
    10861 gcctgacgtc gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg
    10921 atcaaatctt taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt
    10981 agacttgtga tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc
    11041 tcgtcgtaat ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg
    11101 acgaaatcca gtgcttcttg aaaaacaagt cgatgagtaa tcgttgcgcc agattgtttc
    11161 tcaatgaaaa ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt
    11221 tgatcgatgg atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa
    11281 ctatttaaaa aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt
    11341 gtctagttag ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc
    11401 acttttcttt ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac
    11461 gattatgaca ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt
    11521 aagatttatt aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat
    11581 aagtttattt tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt
    11641 cctacatttg ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta
    11701 actcctttag ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact
    11761 gaaaattatt taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg
    11821 acaggaacga tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat
    11881 tggaaagaaa aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa
    11941 tcagctggaa acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt
    12001 tttatgccag aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa
    12061 attaatttac cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa
    12121 aaaattcaaa aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac
    12181 caaaaaacat atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc
    12241 tttttttcta attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc
    12301 tttatttttc aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc
    12361 agttaaacta tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg
    12421 aaaataggat acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc
    12481 ctaaataaat ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat
    12541 cgaccaggat tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg
    12601 cgcttagatc gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat
    12661 gagcgtggtg tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct
    12721 acaggacagc tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc
    12781 ctggaacgat ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt
    12841 cctgaaaaat tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca
    12901 cctattaaga ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg
    12961 gcaaaaatgg aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct
    13021 gaaaattttt gccatttatc tagaaaagtg acaatagtat gagaattttt tgtaagtgtc
    13081 tactgataac ttattctcaa atgttgtaag gattttattc atttgttaat attaa
//
LOCUS       DASHFF010000070        13102 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.070, whole genome shotgun sequence.
ACCESSION   DASHFF010000070 DASHFF010000000
VERSION     DASHFF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.070"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(24..1817)
                     /locus_tag="U2I75_002275"
     CDS             complement(24..1817)
                     /locus_tag="U2I75_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1697371.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            complement(1814..4003)
                     /locus_tag="U2I75_002276"
     CDS             complement(1814..4003)
                     /locus_tag="U2I75_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1697372.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(4137..5327)
                     /locus_tag="U2I75_002277"
     CDS             complement(4137..5327)
                     /locus_tag="U2I75_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEN1697373.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(6199..7830)
                     /locus_tag="U2I75_002278"
     CDS             complement(6199..7830)
                     /locus_tag="U2I75_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN1697374.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7844..9517)
                     /locus_tag="U2I75_002279"
     CDS             complement(7844..9517)
                     /locus_tag="U2I75_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN1697375.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(9530..9847)
                     /locus_tag="U2I75_002280"
     CDS             complement(9530..9847)
                     /locus_tag="U2I75_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1697376.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(9872..11272)
                     /gene="lacG"
                     /locus_tag="U2I75_002281"
     CDS             complement(9872..11272)
                     /gene="lacG"
                     /locus_tag="U2I75_002281"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN1697377.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            11509..12294
                     /locus_tag="U2I75_002282"
     CDS             11509..12294
                     /locus_tag="U2I75_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1697378.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            12297..12989
                     /locus_tag="U2I75_002283"
     CDS             12297..12989
                     /locus_tag="U2I75_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1697379.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
BASE COUNT         4041 a         2666 c         2153 g         4242 t
ORIGIN      
        1 aaaacaacga ctacctaggt attttaaata ttataatcaa attctatttt atctaataat
       61 tgagtgggat aaagcaagtc tcctttactc gtaaaataac ccacctctga gaaaagaaat
      121 aaattatttt ttgctcttga acaaattacg tatagtagtt ttctcgctga actaattcct
      181 ttatcagaaa cattaaaaat ttcattccaa tgaggaattt tcccgtttag aagattaata
      241 gcgataacag tatcatactc atttccttta actttatgtg ctgtagtaag tgtgatacca
      301 tctcgagatt taaacatctt cttaaaacta gatacactac tagataatga attatttttt
      361 atccgatctt ctacttcgtt aataaaaaat agatagtgct taaaaataga cgggtaattt
      421 ttctcgtcaa tattaagatt aacaaatata tgtttggtaa caaatttgta taaatcaaca
      481 ccgttgtcgt ctccactata cattcttttc acactgttta ttagctgtaa taaatctaag
      541 tcaataaaat aatctttaac ttctattcca aattcatttt ttaatataat taaaatatcg
      601 tttgcaatta gctttcttct tcgaaccttt tcacctgatt cagtaaagta caaaattgct
      661 aacttgaaaa ataaactatg attatttacc ttaattggtg atatatttat agcatcaaaa
      721 ttgcaatacg gtaattcttt ctttaaatac tcacccagtg aaaacagagg atagtgttga
      781 ggagcaagta cacaaatatc tttttcatga atgccgcttt caatcttttc ctttattata
      841 ttttttatta tgttgggtaa atcctcctta gtaactttgc aatcataact tataaaacca
      901 cggtcgtttt tattattccc tctgctttca atctcgtaag gacaactctg aaaattagag
      961 tagaagtcta ctaatctctg attagacctg taacagccat gtagcgtctt tgattgaaaa
     1021 gtgtgactag ttagcatttc tagttcttca actgatttag ctataccatc taatgatcca
     1081 taaattgctt ggtcaatatc tccaacaaac attgactgaa tttgagggtt gttttgaagt
     1141 aatttagcta ttacattgta ttgtaattct ctagtatctt gaaattcatc cacataaatc
     1201 gcccttatat tactagcaat attacgtgca acgttactgt ttacagttaa aattttatac
     1261 gctactagta aaatcatatc aaaatcaatt tctttatttt tttccaacat agtgtgatac
     1321 atttcagaaa cttgagtata ctgaacttca ttaattttaa aatcatgaga aagtgacaaa
     1381 tcaattttgt tcagttcata cgttttgaga tttaaattca gcatattatt aatttggctg
     1441 acgtaagctc tttgagtata atcatcaata attgtaaatc cttttgatac tcttgagaga
     1501 ttcattttaa aaggataaat aataaattca agacaaaact gatgtattgt ccccacccaa
     1561 atattaatat catcatctat actaagtaaa cttaggcgtt ccttaatttc ttcagcagct
     1621 ctattagtgt atgtgatagc aattattctt tttagtgatc catcgtgttt aattgcttgg
     1681 taagcaattt tccttgtaag tacccttgtt ttcccactcc caggacatgc atttacataa
     1741 aggttatctt cactttccac aacatctttt tgttctaaat ttaaatcatt tacgattttt
     1801 tcgaagatat ttgtcatttt gaaacctcag cataaagtat ttttgtgtaa ttttctatct
     1861 gattgattag atacacaaca tcctcatctt taccaaagta ctctaataaa gaaattattg
     1921 ctatattcaa atcagctgta tcacttgttt ttaaactagc tgataaagct gacaattctg
     1981 tagtttcctc gtacaagtcg attgtatgga gtagaatttt tttataaatt gagagatcta
     2041 ctatttcctg acatgcaaaa gcaatagcat taagaatata cttaggaatt ttgattaaaa
     2101 agtcaatttt ttcacctaac tcaattgcaa accacccctt accaacatga ttggctagtt
     2161 ttaacatcgc taaatttctt tctaaatatt gttcactttg tatttcttct ttataagcag
     2221 ttattgtttt tttatctttg aatgcgtcat ctataattgg actgacataa gttagaatat
     2281 tttctttatt cacatctaaa aactcaattt caaatgttgt attagcataa aaacaattaa
     2341 cccaattgtt ttcattaaaa agggaggata atttttcttt tcttgcttga cctttctctt
     2401 gtgcagtgct tttatacaat cgatgttctg atgggattgc ctgttcatct aaatcagtta
     2461 caatagcaca ctttctctgt attctatcat cgtcaaacaa gctagcgata tattcaaacg
     2521 aagtactacc aacattaatc aaacctattc caagttcgtc taagtttata ccaaataatt
     2581 tttttactat ttgaggaata agtatttctt cagcatctcc ttcaactaag ataacacctt
     2641 tagaaaaaag caatccattt cttttaacat ctaaatatct ctctactgcg tttattagat
     2701 ttagattgtt cttttttaac ttttctttag caaatgagtt cagattattt gctggattca
     2761 taacgataga tttgccatta aacgatttta atacattcat ccgagatatt tcagacactt
     2821 cagctaaatg tacagaatgt gtggtcatga gaatctgagt atagtttttt tgaagattta
     2881 atccttcgaa cagagttttt tgaatatgat gatgaatatg agcttctggt tcttcaatca
     2941 acataatatt aagtagttct cttgaacgac acgcttcaaa ttcgactatt ttcaaagcta
     3001 aataaatcat atttagatgt cctaacccta gtaagtctaa gtcgatattt tgttctggct
     3061 tcattgaaag aaattttgct aaagaaccca tatcatctga catagttgag gaaagaagaa
     3121 gattaggaga atacactgaa ccaattatgc tatttagttg ccgattaata ttttcgccaa
     3181 ttttcctaat ttcaggaaca ctggtaatag tctcatttaa ttgctgtata attgttttta
     3241 atgactcaac attatctgat gatatttttg attcaattgt ttccatgatt cttttgactg
     3301 ggtttcgatt gtttttcatt tctctaagaa catctcgaag agcgtcaaca taaacaaccg
     3361 aaatatgctt gaaaatatct gacatgtcaa ttcttgagcc caacagagct tcatcgtctt
     3421 cttcaggatt actagctata ttgtcattga gtcttcctac caatttatca taattttcat
     3481 tgatacagaa atttagattt gatttagatg tgtagtaaaa ttcatagtcg gatagacgaa
     3541 tagaatttct aaattcgtta aattgatgag agtctaattc agtggaaata tcaaataatt
     3601 gctttctaat agctttgtta ggtctaataa atagagaaat actacctaca cctttatctg
     3661 catttgaaat caattcttct aagattgttt gactttcaga atttaaagaa atggaagcac
     3721 aaatctcatt gtcaaattct tcttcgctga ttccttcaaa tgtcgcagag ataataatcc
     3781 aatgcccttg ccacccattt tgaattccat gaaaaaaatc tgattctttt agtgttttac
     3841 tactatagta ataactatca tctaataaga ttctcaatgc agtaagagca ttggttttcc
     3901 ctgaatcatt ctcacctata atagtatttg tctcattatc gaatcgaaag atttcggatt
     3961 cataatttct aaaatttcga atttgaagag tagatataaa cataagcaga tctcctaact
     4021 atttaattaa tatgattata tagtaagttt atcataaatt atgttagtaa aagttctcat
     4081 tataagggag cgtcaataat tttgtgtaaa tgattcttcc ctactgcaga ttgtttctaa
     4141 ttctcaatca gttggcttag catttgttcg agctcgcctt cggcctgttg gaaaccttta
     4201 tgactgcgac ctaggaattt ttggttgtaa gtatcaaaag acgagactaa gaaacgttcc
     4261 atcgactctt cattttggaa ctgttccttg cggtggctgt atttcttgat ttgtttatta
     4321 aaggattcaa tcaagttcgt agaataaata ctcttgcgta tggccaaggg gaaatcatag
     4381 aaagtgagca agtgatcgtt cgaaagaatc gattcaacca cttttggata gctggttttc
     4441 catttttccg caaaagtacc acgtgcttcc aacgccacct ctttagatga ggcttgatac
     4501 accattttaa aatcatcaca gacttcctta cgatcatcga cacgcacttt atgactgata
     4561 ttacgggaaa cgtgtacaca acaatgttga aaacgagctt tgggatagaa ccgactaatc
     4621 gctcctacca tcccctttaa gccatccgtg atgaacagga ggacattttt caaaccgcgc
     4681 tcttgaaggt ccaataaaat ttcctcccaa atcgtgatgg attcagtcgg tgcaatcgca
     4741 tagctcaata cttcctttga tccgtccggg cgaatgccaa ctgcgatatg aatagcttcc
     4801 ttggcgacgg ttttccgctt taacggaata tacgttgcgt ccatataaat agcagcataa
     4861 cggtcatgaa gctcccgccc attaaacgcc gttacctctt ctgtaaatga ttttgttata
     4921 ttggacatgg tttggggcgt gtagtaatgc ccatacattt tctcaatcaa gtctgcgatt
     4981 tccgacatgg taataccttt tcggaagagg tgaatgacgg tctcctctaa cgtgtcattc
     5041 gtccgtctat aagcaggaac agtctgttgc ttgaactcac cattgcggtc gcgcggaatc
     5101 tggagatgaa gttccccata ctcggtcttg accgtacggt cataggagcc gttacgagaa
     5161 ttacccgtgt taaaaccaat gcgatcatac ttttcgtaat ctaggaaagc cgttaactcc
     5221 gttttgagaa gcgtattgac tgctttctcc aaatggacac ggaataattc attcaaatcg
     5281 cctttgttcg ctagagtctt tagaatttct gtagtaaaat cgttcatagg gaagtcctct
     5341 cttctgtgaa tgtgttgtgg taactctatt ctacagaagg gacttccttt tttctattta
     5401 cacaaaatat tttacactct ccattataac aattgggtta aaaagaaaat agatagtatt
     5461 ctacctcata tttatttgaa agtaatatat aaaagacgtt caaattcgaa cgcctctcgt
     5521 taaccaaata ttggattcag aatctagttt tcatatataa acagagtatg attaggtgca
     5581 aaaattgcac ttttattgca cttttatttt tatttatagt gttttactgt tctgtatatt
     5641 gttttaactc ccttgatagt atcgcattct gttttataca ctttacgttg aaaacagagt
     5701 gggaataaag agccaacata atatggaggg ggaatcccta taaatcaaca ttcttcaata
     5761 gctaaaatgc ttgattttca ttaaaaataa gatggttccc taccaaaaga gactataata
     5821 taagttaatt tgtttcattc ttatcataaa aagaagttct cgaaaaacga actgttttcc
     5881 gagaactttt ttctatccat ctaagtgctc aaatcatatt cacgacggaa ttctaaggtt
     5941 accctttctt aaacaaactt tatgttatct gcacaacttt ttgcaatttt ttcattgctt
     6001 cttacgaaat cgttgaactc tctttagcat aatacaaagt gtagatgaag tctggtgctt
     6061 tactcgtttt ggtaaaaatg actggaaatc gtttctggtt atttttggca aaggcgaggt
     6121 gcaggttcga ttcctgcctt ctacgctaac gatgtggctg aagggaacct ccgatcttga
     6181 atcgaaacga tccgcagtct attgtttcac caatgaaaca aaatcaacgt tcgtccaagc
     6241 atttcctgaa gccttgctat aaaccccaat ttcacaaaca cctgttgtaa ttggaatgtc
     6301 tttgattgta atttttgtcc attcacttgt tttaacaacc gcagtattta gttccgctcc
     6361 accataattt ttgatatata actgattagt atcaaactct cccccagaca ttacataggc
     6421 actcaatgtg taagttcctt tgcttatgcc actaacattt tgataagctg atgcctcgta
     6481 tgcttgatcc tgccaaaaag ttagtttgta gtccccaaaa aaagtgcccg cttccgtttt
     6541 gatcgtaccc ttagcggttc cttgttgaaa ccaggtttgc caattggaag gactggtcgt
     6601 tggtccatcg ctttcgaaac ttgcattttt aactagattt atttgcgccc ctgcttgttt
     6661 aaacgcacgc aagcttggca aggcgtttcc agcaaaatca aacatcgctt ggttgtccca
     6721 ttcgtttccc atagtggatt gaacactgag ataattcata cctgcttgtg ttgcccaact
     6781 cacatttccg ttgtaccaaa gaggttccca atagaagaag cccgccccgc gattattcgg
     6841 tactgcctga atccgatcgg caagatccag caaatactct gcttgtcctg tcggtgttgg
     6901 ttcatatccc cccgcagctg cttcggcttc accaaaagca tttggcatag tatcggcatt
     6961 cgctgttgta tgcccataag ctgtttcaac tacgatcact tctttaccat accgttggct
     7021 gatcgcattc atattggttt gaagttcatt cattgaaccg tgccaatacg gataatacga
     7081 cactccaatt aaatcaaatg acacacctcg actcgtgatt tcatcaaacc accaagtaaa
     7141 tgctcctgta tcgccaccat cagctaagtg gagcattact ggagttgttt taggttgagt
     7201 gtctcgaaca gcttggatac cagacttcaa aaaagcagcc aatcgatcaa attctccccc
     7261 gccttctccc caagacttgc cataaggcca gagcattccg ctattcagtt cattgccgat
     7321 ctgaaccata tcaggataaa cgtccagctg cttcatttcc gacagaacgt tctttgtata
     7381 gtcgtacaat gccgtattca tttcttcaaa ggtcagacct tgccatgctt taggaagatt
     7441 ctgtttccca ggatcaaccc aaaaatcact gtagtgaaaa tccagcagca ccttaagtcc
     7501 cagatttttt gctcgttttg caaggtcaat cgtcgtttcc agatcatttg tccctgctcc
     7561 ataactttct ccattactat tataaggatc ctgccacaaa cgcagtcgaa catagttgac
     7621 accattattt tttaaaatct ctaaggcatc ttttgaaata tcgtcttcat aatattttgc
     7681 accagatttt tccatatctg acaggatcga aatatctgct ccgcgaataa attcgctggc
     7741 agatgcttca acagtttttg aaacagcccc caaagcccct aacacagctg caaataaaac
     7801 agctttacgc aaccaaattc ccattttcat tatggctcct cctttattga aattggttca
     7861 gaataaactg aacagctttt tcaggatctc tcgttaatcc gatatattct gctccttttg
     7921 tcgcaaccag cttgattcct aagcgatcgg tgtcttcttt gatatcatca tagtaagaat
     7981 tgacttgcgg tgctaagacc accatatcaa aatcctgcag catttcgtaa tgagaaccat
     8041 aagcacccgc cattgcactg atcggtgctt tcgattcaga tgcacctttt tttaaagcat
     8101 tagctaacat cgcacttgtc cctgcgccag cacaaagtac caacacattg atttctttat
     8161 ttgcatcgct gataacttga ctcgctgcca caggtacttc ctcacgtaca tcggcagaag
     8221 caccttcctc ggttgcgatc acctctgctt tttgtgcttc tttctctact aagacagcat
     8281 cgtatgcttt gcaaaatggt agatagatca ggaagtcaac gacgattaga accgctgcta
     8341 gaacaaaagc taagataccc atgcccgttc ctagaaccaa accgatcggt gctggagttg
     8401 cccaaggcaa aacgtacata aagctgttca tgcccaatac atcgacaaaa aacttgaaca
     8461 ggcaaacgtt tacaacaggt gttaacaaaa atggaataaa aaaataaggg ttcaaaatca
     8521 tgggtgccgc aaatagcaat ggttcgttga cagcgaaact tactggaata aaggaagctt
     8581 tcccaactgc cttcaactgc ttagacttag caaagagtag gaagataaaa ggaacaacca
     8641 atgttgcccc agttcctccc atagttccta caaaattccc taaacccacc gtcagtactt
     8701 tagaagcttg ttcaccggaa ttgaacaatt gcagattggt ttccacattg gcatagataa
     8761 ttgccgcaat tgccggctcc acgatcgacg gtccatgaac accgacaaac caaaacagcg
     8821 ccatcgctcc ccagataata gcgattccta agtacccgtc agctgctgta aatagcggct
     8881 gcagcaccgt aatgattgct tctgcaaatg agatttggaa gatttgtcga atgcctaaat
     8941 caattaaaac ggccgcaaat actgaaaaag agaaaggaaa aatatctcgg aaggtttgcg
     9001 aaatcgttcc aggaacttct ttaggcattt taatggtaat atctttcagc acacaaaact
     9061 tatataaatt aactgttaaa aatgcagcga caaaggaagc cagtagtccc tttgtcccca
     9121 tatattccgt tgaaaatcca ccttcaatcg gagagacgct taacaacagg aaacttacca
     9181 cagaggccag catcacagag gtggtgttga ttactttgtt tttcggtaag cgacgattca
     9241 tcgaatcagc caagcagcga gcagtggttg ctgaaacgag taaccctaca acacccatcg
     9301 aataattata gactttccat agccatgtgg agacagattc aggccattgg taaccaaaaa
     9361 tctcaggaac cgcagcaatc aaaatgaaaa tgcttgaaaa taaaatagct ggcatcgctg
     9421 ttaaaaatcc gtcacgaacg gctcctaaat aactattacg tgagattttt tcaaaaaatg
     9481 gtttcccttt ttcgatttgt ttaatgattc catccatagc gttcttctcc tatccttgat
     9541 tctgtctgta cagtacaata aagtcttgaa tcagatctct cagcaataaa gtggtcatta
     9601 aatgatcctg cccatggata aaaatgaatc ccatctccat ctctttccca gatgcttctt
     9661 ccgctaaaat ttttgtttgc gaattatgtg caagggttaa attttcgtcg gcttctttca
     9721 acgccgaatc aacatgttca aagtttcctt gtcttacttc tttcaataat ttcaataatg
     9781 tactgcgagc gtcgcctgaa tatgcaacga tctcaaaacc aagcatttgt aattcctctt
     9841 tattcatctt ttcttcctcc attatcactt attaaataat cgttttggtt tcactgacca
     9901 acttgtacca gtaagctgat tcttttgggt aacgcttctg ggtttcaaaa tcaacataaa
     9961 acaagccata ccttttgtta tacccatttg tccatgagaa aagatccata agcgaccaca
    10021 aaaaataccc cttcacattt accccggctg ttatcgcctt gcttaaggat tccaaataga
    10081 ctctcaaata gtcgattctc ggctgatcca taataatgcc atcttcaaac tgatctttat
    10141 accccatccc gttctcagtg atatagattt tgttgtaatg tgggtaatcg cttttaattc
    10201 gcagcaacaa gtcatataga ccttctggat aaatcagcca gtcccaatca gttcgaggaa
    10261 ttccttcttt ataaatacgt tctccgattc ccttcacttt ataaacagag gtcccttttt
    10321 cacccgtacc gttatgatgg attgcatttt ctccatcgta agctttgaca aaatggcatt
    10381 ggtagtgatt gatccctaag tagtcattac gcgtggatgc ttttttcaat tcaacgaaat
    10441 cttcttcagg aaaatgatag ctggcctgat ttgcttcaca tatctcatca agagcagtca
    10501 aggtttcagt tgaataatag cctaaatagg tagcatctaa taagaaacgg atcgacaatg
    10561 catcgtctaa aaaggcagca tgcttatctt ccggcgcgtc tgttgccgca tatttcgtct
    10621 ctaatgagtg aactacacca atttcacccg gaagttcgtt ttctttgaaa tagttcacta
    10681 cacgggcatg agcaaccatc atattatgaa gacaggcgac gatctttgta aaatcatatt
    10741 tgattcctgg tgggaaaaca cctaacaaat attgattggt tgcaactgga tagatttcat
    10801 taaaggtact ccacaccttg acttctttga attcatgaaa acaaaaaatg gcataggaaa
    10861 caaatgcttc tattgtttct cgattcaaaa aatctccatg gtcaaacaaa cgtttagggg
    10921 tatcaaaatg atgcaacgtc acaaacggcg ttacgtttct tttcttacat tcttcgaaca
    10981 cacgatgata aaaagcgatt ccttctggat ttggtttccc agctccatca ggaaaaatgc
    11041 ggctccaagc aatggataat cttagaccat ttacaccaaa acgttcacac aattcaatgt
    11101 cctttgcata ttgatgataa aaatcacttg ccggatcggg actgaatcgc ccttgttcct
    11161 ctaaaaagtc atcccaagca acagcgcctt tgccaccttc cttagtggct ccttcaacct
    11221 gataggctgc tgtggctccg ccaaaaataa aatcttctgg taatttaagc atgtacatct
    11281 ctcctcaaat ctataatcag ttttcgctct ttctaactga ttgcattgta aacgcatcca
    11341 aaacatttgt caacaaaaac aaacacaaat aatcataaaa accagaaaac agcaataaaa
    11401 tctattgtaa caaacataaa aaccacccta aatgtttact tttgttcgaa ttagattgac
    11461 tgttgtccgt ttatatgata gattatcctt aaattagagg tgaagacaat gttgaaaaaa
    11521 gaaagattgc ttactatcgt agaaatggtc aataaaaaag gaatccttac cgttaatgaa
    11581 atcatcaatc aattagatgt ttctgatatg accgttcgca gagatttaga tgaattagaa
    11641 aaatcgggca agctgctgcg tgtccatgga ggggctcaaa gtctttccta cactttagat
    11701 caagaacttt ctcacactga aaaagccact ctgcagattc aagaaaaaaa agaaattgct
    11761 gccacagcag cctccttgat tcacgaggga gaaacgattt tcttaggacc aggtacaaca
    11821 atcgaactat tggcgacgca tttactgaac aagaaaattc ggatcattac aaataattat
    11881 ccagtttttg atattcttgc tcaagcagat ggaccagaaa ttctactgat tggcggtgat
    11941 ttccggaaaa ataccggcgc atttgtcgga ccgatcacca acgacaattt aagaagacta
    12001 aagtttacaa aagcatttat cagttcaaat ggcgttcaca acgaagcgat ctctacttac
    12061 agtaccgaag aaggagaagc acagcagatc gctctaaata attcccgtaa gaaatatcta
    12121 ctcgttgata ataaaaaatt caatcgagat gatttttatg tgttttataa tttgcatgat
    12181 ttcgatctgc tcattacgga tcgccagatc tcgcgggagg ttcaagagca ctatgcacaa
    12241 tatgtcgatc tgcaaattgc tgattcaacc gaaaatacca ctataggagc gtaactatga
    12301 aagggattat atttgatttc aacggaacaa tgtttttgga ctctcatctg catgaagctg
    12361 cttggctgca tatgatacat gatcatacaa aagatggttt atcagacgag gatattttaa
    12421 aaaatattca tggtcggacg aatacggaga ttttgaaaca tttcatttca gagcatttga
    12481 ccgcctctga gatcgccgta ttgtcagaag aaaaagaaag ctattatcgt atgctttgct
    12541 tgcaaaatga aaacgagctt cagttgacaa aagggcttga agcgactctt gaccagctga
    12601 ctgaccaaca gattccatta acgattgcca cggctactgt caaagagaat gttgcttttt
    12661 attttgatat tttcgattta gcccgctggt ttgactggga caagattgtc tatgacgacg
    12721 gctctttccc tgggaaaccg caaccggata tttttcttaa agcggcagac aaactgagct
    12781 tagctccggc tgattgtctt gttatcgaag atgcttactc aggattactt gctgccaaac
    12841 gtgcggggat cggaacaatt attgcaattg atccattcgg taaaaacaaa acagtatttg
    12901 aacaggcaca gctaaataaa gatggaacca tcaaagattt tactggtttt tggacagagc
    12961 acctgtctgc cacatccgtt aattcttaaa cgattcaact aactaccaaa gaaaagcaac
    13021 tgaaatgcac acctcctttt ataaaaaact gccagtgaaa cgacatgaaa gatacactgc
    13081 tatatcctta gtgaaatata gc
//
LOCUS       DASHFF010000071        13084 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.071, whole genome shotgun sequence.
ACCESSION   DASHFF010000071 DASHFF010000000
VERSION     DASHFF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.071"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(78..2198)
                     /locus_tag="U2I75_002284"
     CDS             complement(78..2198)
                     /locus_tag="U2I75_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1697380.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="U2I75_002285"
     CDS             2428..3606
                     /locus_tag="U2I75_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN1697381.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="U2I75_002286"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="U2I75_002286"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN1697382.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="U2I75_002287"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="U2I75_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN1697383.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="U2I75_002288"
     CDS             complement(4959..6212)
                     /locus_tag="U2I75_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1697384.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="U2I75_002289"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="U2I75_002289"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN1697385.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="U2I75_002290"
     CDS             7789..9135
                     /locus_tag="U2I75_002290"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN1697386.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="U2I75_002291"
     CDS             complement(9443..9829)
                     /locus_tag="U2I75_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1697387.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="U2I75_002292"
     CDS             complement(10097..10246)
                     /locus_tag="U2I75_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697388.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10708..10920
                     /locus_tag="U2I75_002293"
     CDS             10708..10920
                     /locus_tag="U2I75_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697389.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="U2I75_002294"
     CDS             complement(11198..12997)
                     /locus_tag="U2I75_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1697390.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaa
//
LOCUS       DASHFF010000072        13052 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.072, whole genome shotgun sequence.
ACCESSION   DASHFF010000072 DASHFF010000000
VERSION     DASHFF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.072"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(28..2067)
                     /locus_tag="U2I75_002295"
     CDS             complement(28..2067)
                     /locus_tag="U2I75_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN1697391.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2125..2682)
                     /locus_tag="U2I75_002296"
                     /pseudo
     CDS             complement(2125..2682)
                     /locus_tag="U2I75_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(3221..3427)
                     /locus_tag="U2I75_002297"
     CDS             complement(3221..3427)
                     /locus_tag="U2I75_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697392.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(3443..4057)
                     /locus_tag="U2I75_002298"
     CDS             complement(3443..4057)
                     /locus_tag="U2I75_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN1697393.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(4204..4887)
                     /locus_tag="U2I75_002299"
     CDS             complement(4204..4887)
                     /locus_tag="U2I75_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1697394.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(4996..5454)
                     /locus_tag="U2I75_002300"
     CDS             complement(4996..5454)
                     /locus_tag="U2I75_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN1697395.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(5538..7301)
                     /locus_tag="U2I75_002301"
     CDS             complement(5538..7301)
                     /locus_tag="U2I75_002301"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN1697396.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(7522..8982)
                     /locus_tag="U2I75_002302"
     CDS             complement(7522..8982)
                     /locus_tag="U2I75_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697397.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(9190..10116)
                     /gene="glsA"
                     /locus_tag="U2I75_002303"
     CDS             complement(9190..10116)
                     /gene="glsA"
                     /locus_tag="U2I75_002303"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN1697398.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(10139..11566)
                     /locus_tag="U2I75_002304"
     CDS             complement(10139..11566)
                     /locus_tag="U2I75_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1697399.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(11597..12811)
                     /locus_tag="U2I75_002305"
     CDS             complement(11597..12811)
                     /locus_tag="U2I75_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN1697400.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3751 a         2720 c         2177 g         4404 t
ORIGIN      
        1 cttttactgt acttaaaaag caatttttta ttgataattt tggttgaggt attgcaatag
       61 ttccgatgct cgtttggttg ctgaatcatc atcttcttta ttttccaaca tgctgaccat
      121 catataacct tggttatcaa gattgaaagc aaacaagaaa ctgttctctt gcccctttac
      181 atcctgtttt tctttgattt ccgctgtacc agttttcgct gccaacggaa tccctaaagc
      241 agaaagagaa tgtgctgtac catttacatc ttgcacaact tctctcagat ctggcacgat
      301 cgtttgtact gctgtttcgc tgatgacatt cttcttatct tttgtctctt tatctgcaat
      361 caatttagga tagacaagtg tgccattgtt gataaaaaca gaatacattg ctgcttgctg
      421 gataggagta attagaagtt cgccctgtcc atatccagta tcagctagca agatatctga
      481 gttaaagcta tcttcattag aaatttgtgc tggattcata ctaattggca aatcaaggtc
      541 ttcaccaaaa atgaatttat ctaagcctgt acgaaatttt ttctcaccca ttttcaacgt
      601 ttcttgtgcc gcatatatat tatcggaata gatcaaagca gtttttaagt ctacttgtga
      661 tacatcacta ctgacacgcg ttgttttaaa agatccccaa gaactgtctt tttgccattt
      721 aagcccgttg atcgtcaaca cttcatttgg atcgatagtg ccgttgtcga gaccgattgc
      781 tgctgtaatc attttaaacg tagatccagg agcataacct gtcgcaaatc ggctgatgaa
      841 tggatgttca ggattttctt cataagcttt gtaatcttct tgtgagatcc cgtttgtcat
      901 tttgtttgga tcatagcttg gagagctagc aagcgcaaga agatcaccgg tttttggcgt
      961 tgtcgcaaca gttgatgcag cttttcctcc tagactgtca aaagctattt tttgtgcctt
     1021 cgcatcgatt gtcaatttga tatctttgcc attttgaact tcatgttcga tcagaacttt
     1081 tttctctaca ccgtctgcat ctgtaatgct taatttccca cctgttgtcc cacgaagatc
     1141 cttatcaaaa gccatctcca aacctgagcg accgattttt ccattactgc ttaattctgg
     1201 attcttatca atatcttctg ctgtgatatc tcccacgtaa ccaatcagct gagcagctgc
     1261 ttcacccaaa ggataatatc tgccgtctac ttcttgaatg gtagctccag ctggaagttc
     1321 tggcgttgct ccatcaatga ttttcaatgg gacaaagtaa tcgggttgta cccagctttg
     1381 tgaaatcgcc tgattgatag catcttctgt taagtcgaag gaggaagcaa tcgctttgat
     1441 attggctgtt ttttcgcctc catctccaag tttacttgga acgactccta attgttttag
     1501 tttgcctgtt gttgctaatg gttcaccatt tcgatctata atattccctc ttgctgcttc
     1561 ttgtgtggtc aggcttactt tgtcatttcc ttccatttct ggaaaaacca agttgggttg
     1621 ccagttgatc gtggtttgac catcatttct gtctaatgtt cctttataag aaagatcttt
     1681 caattcacct aatgaggtat tcatctttgc tttatatgaa aaactgtaag tagaatcgtc
     1741 ttttttatct acttttagat tcgatatctg aagtcctttg acatcagcag caccgtatat
     1801 attctggtat ttatctaaga tctctttttc agataatgca cttttatttg ccgccttttt
     1861 tgatgtcatt tctgcagctt tgttataatc tcccttgttc aaagcttgga caaattgctc
     1921 aactgtcttt tctccagctt ctactgcttg ggtttcttga tagtgctggt agtaaaaata
     1981 accgccaccc gcagctgcta ttaagatcac tgcgccggca atataagcgc ctgttcgatt
     2041 tttgccgtgc ttgtcacttc ttttcatttt ttcctccttc tcttgcttgt cattccttta
     2101 tttcactatt tatacctgtt tattctaaca tatgtctttg aggaaatcca taccacaatc
     2161 cagaagcaca ctttctggaa ggttcgtttg gatactacca agtaatttcc caaccgcttt
     2221 gggtagcttg aatattgaag ttacgtcttt cagctgctga gagatagctt tgatcacttg
     2281 ctgctgcctt ctgatcctcc cgaagtctcc ttctttgtct tcccgaaatc gagcatactg
     2341 gagtaaagta ttaccatcca tcacttgctc tcctttgttt atatatacgc catctaaatc
     2401 tagagatttt tctgcatcga tcttgactcc accaggaaac agttcattaa cgcaatcgat
     2461 aaaatcttga aatgtgatac ttgcataata gggagcttca aatttgaaat tttcctttaa
     2521 tgtttgattt aataaatcaa ctcccccata agagtaagct gcatttatct tattcattcc
     2581 gtaacctgga atatcgacat atgaatctct catgatagag atcagttttg cttgttgtgt
     2641 tttctggttg tattgtgcaa ccagaagtga atccgagcga cctccaccaa tcgatctttt
     2701 ttcaccgcat ctcctcctca cacagcaatc cacttgtcag gtgaggagcg agtttgtctc
     2761 atcaaataag tcagtcacaa aaatataaag aaaaggtcag aaaccttttc gaacaagaat
     2821 tctgacctag catcatctca tcgtccgagt tttagcacta tacaacgtaa agaaaacatt
     2881 cttagctatt ggaaaaagcc ttaatccaac agtttctgtt ttacccattg gcgactttcg
     2941 acgtttctgg gcaacagcct atgtttgtat aggagcctca cctaacaagg ttattactat
     3001 attctattta cgagttcatg atagactttt cctaatgatt aggcaatgga atttgaaatg
     3061 aaatagtgaa atttaacaat ctttaatatt ttttagaaaa aaataacaaa taagttgaaa
     3121 gaatcccgtt gcaaattctt tcagttgcac caggattctt tgataatttt ttagttttat
     3181 gtgttttttt atatgttttt tcacttttcg gaataaatat ttatttgtta aagtaattaa
     3241 acaatccgct aatcgtttga tctggcattt ctatttcctt accatctaaa gtaacagtag
     3301 taacgacaaa atggtcttgc aatggcggaa cttctaagta ctgcccttgc agaaaatcat
     3361 tcactgtttc atattctttt ttcatttctt ttgaatctgt cgatggttta tagcttattt
     3421 ttaacataac tagtgctcct ttttattcgg gacgtgcaat cgtgaaaatt tctcctattg
     3481 ctgcataatc atttcctgcc aaacggctta cagcattcaa ttttcttgga tcgatccttc
     3541 ctgtcttatc atcatagact gtttcattta aatgatattt ctgtacttct agcaaaaata
     3601 aatcagctgt tactttattt ttattccgta tctcgacatg ttcatataag gatgtttcaa
     3661 aacgaacttt tgcttctgtc aatgctggta ctgaaatgat tactgaatca gttaaagtca
     3721 agtcagtcag gtacagttcg ctttcctcat taggcagatt ggctgctgtt ttatttactt
     3781 cttctacgtt gtcctcatca aggatatgga taacagcttc tcttgtctca agaatattct
     3841 ttgctgtgtc ttttactgca tcttgttttc tctggatcgc caaggataag atgggtcgat
     3901 tcgaactgac gatattgaag taactgaagg gtgcgatatt cactatatct tttgctgatt
     3961 tagtcgttac tactgctact ggtctaggga tgactgaacc aatcagcaac ttgtaatttt
     4021 cacgttctgt caattctgaa ggactccgtt caaacatggt ttcgccttct ttctataaaa
     4081 ttcgttaaaa agcagatctt gaagacaaaa tgaaaagtaa tgcttttttc atcttcttag
     4141 aatctgcttt aaactatcta tcaatttttc ttttttgtca agaatctgtg ccgtgcgtgt
     4201 tttttagtta tttgctttac ttgcggcttc gacatatctt ttagtgatca attgcggacg
     4261 ggtgataata gatccaacta cgactgaaaa agcacctaat tcatatgctt tctctacttg
     4321 ttcaggagag tcagtatgtc cttccataat tactgggata tcgacttgtt ctatgatttg
     4381 attcaataaa tcaaaatcag gtgtctgcgt ctttcgtgaa tattctgtat agcctgctaa
     4441 agtcgtagac aaaagatcaa aaccatcttc ttgcgcttga agcgcttcct caaaagtcga
     4501 aatatctgcc atagctaacc gaccgtgttc gtgaatcatt tggacaagtt cagcagttgt
     4561 tttttctgtc ggacgtttac gagaagttgc atccagtgca atgatttctg cttgcgtctc
     4621 caaaagttct tccacttctt tttctgtcgc agtaataaac acttctgacc catcgtaatc
     4681 tcttttgata atccctatta ccggtaaatc aacgattcgc ttgatttcat tgatatcttc
     4741 tgtcgtattt gctcgaatac caactgctcc gccttctttt gcagccaaag ccattctccc
     4801 cataataaaa gaactgtgta gcggctcgtt ttccaaagct tgacaagaaa cgacgactcc
     4861 gcctttgatt tgttccagtg tatccacaat atctcctcca gtaatgttat ataaaaatac
     4921 agcttcatat aaaaatgtaa cccttttcta ttcaggagac aataatgagc tatttctttc
     4981 agcagacctt tcttactaaa agaaaattac ttcaaattct tcgtatacga ccagcttctc
     5041 ttctgtaaat tctatgttca acgcttcttt ataagcatgt ttaaaaaact cagcatggac
     5101 tttccgccaa taagctaatg tccgatcccc ttctccttct ttccaagcat gctctttcgt
     5161 gacttcacta aaaggcaaga tggtcacatt tatatttttg atgatacaaa ctggttcatt
     5221 ttttccattc aggaccacgt ctaattgacc aacttttggt aaaggctcat tctcttgttt
     5281 atacagatcg taaccacttg atgtaccagt ctttatcttt tttacaacta gttcgctcaa
     5341 ctcgtctgcc attttttctg tatccccaaa tgaaaagaaa cctaattttt ctgattgata
     5401 actaggatat ttttcttgga actttttgac atattcttct acttgctgat acataacatc
     5461 cacccctttg ctttttataa tgaaaaaacc atccagatac tagtataaac aattatctgg
     5521 atggtgagca taatgaatta ttgaatattc aagacacgca gactatcttc aacgatttgt
     5581 tttggtgtta aatgataacg ttcataaagc tgccctacag gaacgctatc cgtgaattct
     5641 ttctctgcac caaaatttaa tacgttcatt gaagaatcac cataataacg actgatattt
     5701 tcgccaaatc ctcctgacaa actaccatct tctaatgtga tgactaattc gtgtgtttct
     5761 tttaatttat ctaatgtttc tacgtcattt tctgtgatcg ttcctggatt gatcaacgtt
     5821 gcttcgattc ctttttttct aaaagctttt actgtttctt ttcctaattc aagaaaaccg
     5881 cctaaagcta aaattgctac ttttcctcca ttatttactg tttgataggc tggaatacta
     5941 taatcatttg cgatagctgt accatgttct acaccatgtg taggaattcg gattgctaca
     6001 ggctgcgctg tttgttcgaa cgcccaatct agcatagaga tcatatcctc ttttgttgct
     6061 ggagcaagat atttgatgtt cgggatactt gataagtaag gaatgtcaaa tgttccttgg
     6121 tgtgttcggt cactagctga gatcgttccg ccaccgacta aaataatcac tggattctcg
     6181 ttgatcgcta aatcatgtga taattgatca tatgcccgtt gcaagaaagt acttgattgg
     6241 aagacgactg gacgtactcc ctgagaagcc aaagcagaag cgaaagtgat agagtgctgt
     6301 tcagcgatcc ccacgtcaaa ataatgatct ggatgttttt cttggaatgt tttcagtcca
     6361 aaggcgcctg gtatcgctgc attgactgcg gcaataggag ctgtacttgt ttgcattttc
     6421 ttatccaaat aatccatgat gatcatatta aatgtttctc cagaagttac ttgctttgcc
     6481 tccccggtgg acagatcaaa tggtacatgc caatgatatt tcattttatt atcgattgcc
     6541 ggctgataac cgcgaccttt ttccgtatgg acgtgaagta caatcggatg atcaatatct
     6601 tttacttctt caaataaacg gatcaccgtt tccagatcgt ttccattatc cacgtaacga
     6661 taatcaaatc ccattgcttt aaataggtta ttgctgcttt taccgttggt ttgacgaagc
     6721 tcagctaagt ttttatataa actgccatga ttctcatcaa tagacatctc attatcgttt
     6781 actaatacga ttagattgga ttgtaaaaca ccaccattgt tcaatccttc aaaagctaag
     6841 ccaccactta gggagccgtc accgataaat gctaccacat ttcctgattc tttcttcaga
     6901 tctctcgctt tagctaaacc tgttgctaag ctgatagatg tagatgtgtg ccccactgta
     6961 aaatagtcat gtttactttc atgttggttg gaatatccgc tcacttcacg gtaatgtcct
     7021 ttttcaaaac cattttttcg accagttaaa agcttatgac cataagactg atgggaaaca
     7081 tcccaaacga ttttatcctt tggtgaatca aatacataat gaaacgcaat cgttaattct
     7141 actacaccta gattcggacc aacgtgccca cctacatcgc tgtccttttg caagattttt
     7201 tggcgcatct cttctgccaa ctgtttcatt tcctcaacag ataatttttt gacatctgcg
     7261 gggctattga ttgcatccaa tacatgtaat actgtactca taatacattc tctcctccgt
     7321 actatcttct tttaatgaaa atagatttgt tacactttat ctattctact acttagagtt
     7381 gactctaagt caatagcatt ttaaaaaaat ttcagagaaa gcttgttata taagtagatc
     7441 ttcgcacgaa tcaggactca aataaaaacc ttctacagac aattaaaaag ctttgtagaa
     7501 ggtctcctaa ttaaggtctc atcaagaaat atgttttttt aataggtgaa gttcctgagc
     7561 aatatgatac aaatggatgt ttgcaccttt taatgagatt ggatagatct ttgtcttttc
     7621 agtttctaat ccattttgat agctagcagc atcgcacaaa accactcttt ttttcgcaca
     7681 tagacattca tcaggcaatt cttctaatga acgaaataaa ataggattta tttcagcgat
     7741 gccttcttcc gcatacaaat gaaattgggt tcgataaaaa aattgtttga ctgaactttc
     7801 tgaaacaaca aaaatttcta tttcctgtct ccccgcttgt tcactgttga tcaaagaaag
     7861 acttttgacg aattttcgaa tgaaattttc attccatctt agttggattt gatttttctt
     7921 tttccactct tccaatattt ctacaatttt tccgtacaga agttgttctt tttctgatag
     7981 aagatagatc ctttccggta aaaatacttg tccatctgcc cacaacgtac ggacaaattg
     8041 taaaaaggat tgcttgaaaa taaaattttc tgcatcgtcg atttcagcga tactaataat
     8101 ctgtaaaatc aattcattca gtgataggtc attgagaaca aaatttttat aaacaacttc
     8161 gaaatctttt cttaacagct ccatgttctc attggtataa tttctactgt tgaacaaaga
     8221 aaaaatataa tagacatctt cttcttgtaa gtctagaatt cctttttgct cttccaatcc
     8281 ttgacagact aattgaaata acggcttcgt ttctgcgctt ttcttatctt cttctttcag
     8341 tccaagggga aatgcctttc ttccgattgc gatctctatt cctcgtatga taaaggactt
     8401 ttcatcttcc gaatatcttc ttttaaaaaa atgagtctct acataatcaa tcaactcgtt
     8461 acaagcttct tctacatacg gatttttatt ttcatagcct tcccaattga cataacgtac
     8521 taaagctagc aagatgttcc gcgagtcaaa ttctgggata atgatttctt tatttttcaa
     8581 ataatcattt tctttaaaaa aatccaatac gatttttttg attgtatata ctttgctcaa
     8641 agaaataaat tcaagatcag ccagttctgt actcgtgaat ataccattaa aataataact
     8701 taaaaacttt aaaaaataag attgaccgta cagaaactgt gtgagctcag aaagagcaat
     8761 accatgtttg aatacgagtt tatacttatg cttctctctt acttgttctt ttatacaata
     8821 ttctagacac tcggctagac tttctagatc gtgaaggatc gttggtgtag tcacccttag
     8881 agaaatagct gtttgttcga tgtctatttc ttggaattca aataattgtt cacacagata
     8941 cacttttcta aatatatttc gctcgatgta attttccagc attcctgata cacgcctcca
     9001 gtttttctta tgattttatt taatcatatc tgtaaatcgt atttttgttt cctttttatt
     9061 tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg gacaaaagtc aaacaagctc
     9121 acaatttcag cctcattgta ggtagctttg agccgtttgg acttttaccc cagcctcttt
     9181 caagaatggt tattcaaaga tgtcagcgtc tagttctttg actacatctt tcaacaattg
     9241 gataccagct gcgctgttac caaatggatc tagtgccggg ctgaagacac ctatgccgta
     9301 acgatttggc acagctgcca tcaacccgcc gcctacaccg ctttttgcag gtacaccaac
     9361 atggacggaa aactctcctg attcatcata caaaccagca gttgtcataa ttgatttgac
     9421 gattgtagca ctttcttcag taatgatttg tttgccattc caaggagcaa ttcctttatt
     9481 tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg acattgaggg agcattgtct
     9541 gaaataagca tcgagtatct ccggtacgtc tccttcgatc atttgattac ctttcatata
     9601 gtaagcaaga gagcgattga tatcacctgt tcttgattca gaatgataga tttcttcatt
     9661 caatgtgata ttaggatcat cacagatctc tttcataaat gacaagattc gctcaaattt
     9721 ttcatctgca tctttaccgg caatcaacga ggttgttgca atcgctcctg cattaacaaa
     9781 aggattcatt ggatgtttgc gatgattgat ttccatattc atgatagaat tgaaggcaaa
     9841 accggttggt tccgtgttga ttgtttgaaa gactttttct attccgttgt ctttgattgc
     9901 taataacaat acgggaactt ttgacatgct ttcgatggca aagcgcacat cggcgtctcc
     9961 tccctcaatc agttgttttg tatccaaatc ataaatcgcc atacctaatt gtttgggatc
    10021 cacttcagct aatgctggaa tgtaatccgc cacttgtcct gtttgataat agtggaaatt
    10081 ttttccaatc aattgttcta attcttttac ttccatttga ataaccagct ttcatttttt
    10141 atttttctgc atgctggaat ttatgacggc atgcgtagat cgtaaatgga ataactaaga
    10201 cgataagata agaaacaatc agaatcgttt cataaatccc atgctgtcca gctgcgatac
    10261 tagcaggtgg aacaaatgag atacaaaaag caaaaagtga agtcaaaaga cctaaacaag
    10321 caacgattac ttttacaact tttccgcctg gaacctgata ggctctttca agactgtcat
    10381 ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa gtacaacatc aggtagatta
    10441 caacagtcaa tgacatcgct gcaagaaatg aaagattatt ccccccgccg cttaatgtaa
    10501 agacggctgc ccagattgtt gcaacgatcc catttgcgat catcaaatga gcaggaacac
    10561 cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc ttcttctgct gctacgtgaa
    10621 gtgcttttga aggagaggtt acccaagaag caatttctcc gatgacacct aaacaaacaa
    10681 gaatcgcaag aattttcgca atccatgcaa gatttggatc aatgtgtctt aataggaatt
    10741 ctaacgattg aatcacccct gtattaagag acaaaccatg ttgaggtact gtagtagcga
    10801 cggtcaatcc gccaaaagta tctaaaatga ttgccagaat aaccaataca aacattgcta
    10861 atggataatc ctttttaggg ttttccattt catttgcatg actggcagat gtttctacgc
    10921 ccatatatgc taaaataaag gaaacaaaaa caaccagcgt aggcaatttc gaaaaatcag
    10981 gaatcaatgt tttcatagac agtatcgttt gtagtggagc gcctgaagcg acataatgga
    11041 tccctaagcc aaacaagact aatgctggca acaagatacc agctacaaaa ccgatccgag
    11101 caatcaatga agtgtatttg gttcctttta actgtgagag taaaatagcc cagaaaataa
    11161 tcaataggat tgccaatttc agatatttat tgttttgtat agcaccaata cccgttgcat
    11221 aggataatgc accagttaag aaatacagca tggtcaagta gccaactgtt atctcaaacc
    11281 attggaaaaa gattgcggca aaaccgaatc gttttcccaa tgtgcgactg acccaagtat
    11341 agacaccgcc tttttcccat cctttcaccg ttgccatctc tgctgcgcac aatgccacag
    11401 ggataaacca tagcaaaccg cctaataaca agaaaaatac taagctgaag cctgaggtcg
    11461 cgaatgtggg atattcatat aatgaaacga ccattgagat ggtcatagcc aaaaagccaa
    11521 agaacgttaa tttttttgca ggaattttcg ttttgctttg ttgcatgaat cttcctcttt
    11581 tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc aatccttctt
    11641 cttgttcttc ttttgtagta gtgatcgttg taaagtgcat cgccgatttt aaaatcactg
    11701 ttgtcagcac ataggcatac acggcagtaa tgatgacacc aatcacttga atccctaaga
    11761 agtaccagct gcttggattc gttagtaaat ggctgttaga tgcaaagatc cctatcagaa
    11821 tcgtacctgt gaatccgccc atgccatgaa caccccacac atcaagtgca tcatcccatt
    11881 gtttcttttt acgaaaatca acagcagcat gacagacgat acccgcaatg atcccaatca
    11941 gcatggcact ttttgcttct acataaccgg cacaaggcgt aatggctgcc aatccagcaa
    12001 cggatcctgt tagtacgtcg acaaaatcaa agccatttcc ttttactttt gcaatgatca
    12061 gccaagtaat catcgcaaat gcaagagcga tgaatgttga accaaatgca ttgactgctt
    12121 gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac caaagcagtc
    12181 ctgtaccgat agcagctgcc ataagactgc tatgctgact tctttctcct aaaggaatct
    12241 ttctttgacc aagtgtgagg atacatgcta aagagccgaa tccagctgtc gtgtggatga
    12301 ctgctccacc agcgaaatct ctaaatccca tctgatccaa aaatccgcca ccccatatcc
    12361 agtggcaaac agggaaataa atcagcagat tccaaaaaat aacaagtata atatagcctt
    12421 ttaaattcaa tctccctgca aaagctcctg tcatcaaagg aacggtaatc acgataaaca
    12481 tcagttggta aagaaagaac attaaaaatg gaatcgttgc accatgaaac atgttgggga
    12541 agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt tccaataaca ccgccgatat
    12601 cccgtccaaa ggccaatccg aatccaccaa atacccacat aatcgttgtt atcccaacgg
    12661 agagaaaaga ttgagacatg atcgttagta tattattctt ccgtgctaat ccgccgtaaa
    12721 aaaaagctag accaggagtc atcaggcaaa ccattgctgt acataaaatc atgaatgctg
    12781 tatctcctgt attgataggt aatgtattca tttttttcgc ctcctttcta cgattacgag
    12841 tataaaagaa ggtgtagttg tttttttctt ctgttttttt gaattaatac ttgcatttat
    12901 aaaagacaga caattaataa aagcttgatg tgttgtgctc tttttcgttt ttcacacaaa
    12961 taaagacaaa aaataaacgg tcttcttttt atagaaaacc gtttattttc gtcttttttt
    13021 atttttaaag atattttttc ataaggaata aa
//
LOCUS       DASHFF010000073        12874 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.073, whole genome shotgun sequence.
ACCESSION   DASHFF010000073 DASHFF010000000
VERSION     DASHFF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.073"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            69..503
                     /locus_tag="U2I75_002306"
     CDS             69..503
                     /locus_tag="U2I75_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1697401.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(581..1114)
                     /locus_tag="U2I75_002307"
     CDS             complement(581..1114)
                     /locus_tag="U2I75_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN1697402.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1208..2386)
                     /gene="mutY"
                     /locus_tag="U2I75_002308"
     CDS             complement(1208..2386)
                     /gene="mutY"
                     /locus_tag="U2I75_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN1697403.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2379..3176)
                     /gene="recX"
                     /locus_tag="U2I75_002309"
     CDS             complement(2379..3176)
                     /gene="recX"
                     /locus_tag="U2I75_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN1697404.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3283..4665
                     /gene="rlmD"
                     /locus_tag="U2I75_002310"
     CDS             3283..4665
                     /gene="rlmD"
                     /locus_tag="U2I75_002310"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1697405.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4839..5417)
                     /locus_tag="U2I75_002311"
     CDS             complement(4839..5417)
                     /locus_tag="U2I75_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN1697406.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5739..6209
                     /locus_tag="U2I75_002312"
     CDS             5739..6209
                     /locus_tag="U2I75_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN1697407.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6317..7543)
                     /locus_tag="U2I75_002313"
     CDS             complement(6317..7543)
                     /locus_tag="U2I75_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1697408.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8297..8497)
                     /locus_tag="U2I75_002314"
     CDS             complement(8297..8497)
                     /locus_tag="U2I75_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN1697409.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     LEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8631..9239)
                     /locus_tag="U2I75_002315"
     CDS             complement(8631..9239)
                     /locus_tag="U2I75_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN1697410.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9324..9794)
                     /locus_tag="U2I75_002316"
     CDS             complement(9324..9794)
                     /locus_tag="U2I75_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN1697411.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9842..10756)
                     /locus_tag="U2I75_002317"
     CDS             complement(9842..10756)
                     /locus_tag="U2I75_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1697412.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10902..11705
                     /locus_tag="U2I75_002318"
     CDS             10902..11705
                     /locus_tag="U2I75_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN1697413.1"
                     /translation="MTTKQLIISSFIRFKDLKESRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11883..12830
                     /locus_tag="U2I75_002319"
     CDS             11883..12830
                     /locus_tag="U2I75_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN1697414.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         3914 a         2544 c         2122 g         4294 t
ORIGIN      
        1 gaaaacttgc catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg
       61 gctagaccat gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg
      121 tacggatcag gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag
      181 acaaagatga agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag
      241 gaactgtacg tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc
      301 tttctgaata cagacaccaa gggttaggaa aaatattgat tgaagaagca gaaaactttg
      361 ctaagaatca aggctataat acgattttat taggggcaca gtccacagct gaaacttttt
      421 acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc atgcctcata
      481 tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt
      541 tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct
      601 tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga
      661 tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat
      721 tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag
      781 gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa
      841 aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat
      901 tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc
      961 catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt
     1021 ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga
     1081 tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt
     1141 gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga
     1201 gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca
     1261 tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt
     1321 tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat
     1381 gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa
     1441 agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca
     1501 gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca
     1561 cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa
     1621 aataatacgc tccagaatga ttttgaagag ctgcactgat ataataaacg tcttttggct
     1681 ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa
     1741 aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta
     1801 gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg
     1861 cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc
     1921 tgctgactac tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca aatgcgatac
     1981 ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg
     2041 tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc
     2101 gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat
     2161 ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg
     2221 tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat
     2281 tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa
     2341 attcttttgt ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt
     2401 tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt
     2461 acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct
     2521 tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag atcagctatc
     2581 gcttgttgga tcacttcttg agaaaagcct ttcgtcatca atgtttgttg gatttttctc
     2641 aataattctt tttggctttt tccgtgattt tttccaaacg ctttttcggc tgtctgatgt
     2701 gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt
     2761 ttcaaacctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc
     2821 atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt
     2881 aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt
     2941 cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg
     3001 aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct
     3061 tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat
     3121 aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac
     3181 atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat
     3241 atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc
     3301 aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc
     3361 ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta
     3421 gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa
     3481 acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa
     3541 ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca
     3601 ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg
     3661 gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact
     3721 caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc tataacagac
     3781 tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat
     3841 aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg
     3901 gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa
     3961 ttcccgcaaa aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca
     4021 atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac
     4081 ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct
     4141 cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa
     4201 gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc
     4261 ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca
     4321 atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc
     4381 gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc
     4441 atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac
     4501 cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt
     4561 aatttaagta aaaagttcaa gattgaatat ttacagtccg tcgacatgtt tcctcagact
     4621 gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg
     4681 aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt
     4741 tatgaatact gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag
     4801 ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc
     4861 acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat
     4921 ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct
     4981 ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc
     5041 caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc
     5101 caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt
     5161 tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta
     5221 tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag
     5281 tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc
     5341 gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac
     5401 gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta
     5461 ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa
     5521 agtaagatgc agattataac agaagtagac gagaaaagta caaatagaaa acatgagaat
     5581 caaatcatat gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat
     5641 aacaacaatt atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc
     5701 ttcatggtaa actaatatta tagaaaggag ttagtaaaat gaagatgata aaagatattc
     5761 gtttttgggt ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg
     5821 gaaatcccgg aaaatattac tattctttgc aacttccacc atttgcacca cctagctgga
     5881 tatttgggcc gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac
     5941 ttaatcacaa caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca
     6001 tttttaattt tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa
     6061 tcgatattac acttttagtt attttcttgt cagtgcttat gtatcagttg tggctgcatc
     6121 atcgcctggc aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat
     6181 tgaattattc aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca
     6241 catagaaaaa gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta
     6301 accaaactat ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac
     6361 cataaccatt ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg
     6421 tatagtactg acttgtatct tctgtttgct gataataggt attcggatct tggtactgat
     6481 catatgtgtt ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac
     6541 cgtttgtcga atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt
     6601 tcaaattagg taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga
     6661 tcgaaagaag atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt
     6721 cgccttgcaa ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc gtcgctacat
     6781 caagcatgtc atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt
     6841 ttctataatt gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt
     6901 cccgctgacg acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat
     6961 aggctaaacc tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca
     7021 aagtaaagtc aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca
     7081 tgccatccaa attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg
     7141 aatccatggc catttcgaca ccaccaaatg cgtaggcatg atttaattta tctaccgttc
     7201 cataacctac gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt
     7261 gtttcggatt aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta
     7321 atccaccggt atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta
     7381 ctctctcaga acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt
     7441 ggctcgcttc tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacga
     7501 atacccctaa caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca
     7561 cattttctaa aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata
     7621 agaaaacttt aaaaaacatt tttgtgtaac gattattatt ttttaaaatt tctcaaaatt
     7681 cttaactatc ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat
     7741 cgttttatct ttttttatgt attttttcca gaaaaagcga acaacaaggt attttccaaa
     7801 taatcgtgca gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac
     7861 cgttcagatt atcattcgat ttcacgacaa tttcttccta tgactggtcc ttttgttaaa
     7921 cccatcttct attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta
     7981 gtgattgcat taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat
     8041 tttcttttaa agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa
     8101 tggtctttta ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa
     8161 aatgttcctt gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc
     8221 caaaaaatct aaaaacttca taaatagaca aaaagacacg actctctaag aagtcgtgtc
     8281 tttattcaac aaattattat aatttagtca cttcagcagc ttgaggtccg cgtgcacctt
     8341 caacaatcgt gaattctact tctagacctt cttctaggct cttgaagccg tctccttgaa
     8401 ttgctgagaa atgaacaaat acgtcatctc cgccgtctac tgtaataaac ccaaatcctt
     8461 tttcattgtt gaaccattta accgttccgt gttccataaa tgtatatcct ccaaaaaagt
     8521 tcttgcaagt aaccttgcaa gaacattata cccaatttag aaaacgctag caagctttcg
     8581 ctcttttatt ttccaaatta tttttaagac tgatatgacg gaggaaatgg ttattttgct
     8641 gcttcgaaac gtttgtttac ttcatcccaa ttaataacat tccagaatgc tgcaatataa
     8701 tctggtcgga catttttata tttcaaataa taagcatgtt cccaaacgtc caaacctaga
     8761 attggtgtct ttccttccat caatggagaa tcttgatttg cagtagaggt aatagctaat
     8821 tttccatttt ccattacaag ccaagcccat ccagaaccaa atcgtccagc ggctgctttt
     8881 ttgaattctt ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc ttcttttatt
     8941 tctcctgtag gttcgccacc agcatttggt gccatgattt cccagaaaaa tgaatggtta
     9001 gcatgtccgc caccattgtt acgtacagct gtcttgatat ctgttggaat agcgtccata
     9061 tcagataata attcttctat tgttttttcg cctaattctg ggtatttctc aattgctgaa
     9121 tttaaattcg tcacataagt attgtgatgc ttatcatgat gcagatgcat cgtttcttcg
     9181 tcaatataag gttccaatgc gtcatatgca taaggtagat ctggtaatgt gtaagtcata
     9241 aaaaaattcc tcctcaatgt tttcttaata atgtctacat tacaaagagt aacaaagaga
     9301 aggaaattct tcaattgtta tgcctagtcg tctttttcac tttgacctac ttcttcttca
     9361 aaaacctcag tttctttaaa aataaacaac ttgctgaaaa tatagttcgc aaggataaca
     9421 ataatttgag tgatgatttt cgccacatac tcattgctgt tcataacatt gatcaacaat
     9481 accattgccc ccatgtccaa tccgtaagaa aggatacggt agaagaagaa cttaccgcat
     9541 tcaataagtg catgtgtaaa attcggtgat tttgaatgga atacccaaag tttgttcgtt
     9601 acaaaagcaa acaatacaga acaaaaccac gaaatagaat tggataaggc catacttaaa
     9661 tcaaaatatt ggcgtgcgag agcaaaacta acaaaattta caatcgtagc caaaccacca
     9721 aaaaagagat agactagtat ctcccaatat ccttttgctt ctaaatatcc ttttaactgt
     9781 cgatactgct tcatgagcca acctacctta ttaaaaattc ttatttatca tactctatat
     9841 ttcaataact ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt gcctttgtct
     9901 ctgcttggat cgtcacatgt gtaataccat attttaattt taataaagaa attatacgag
     9961 gatatatctt ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa
    10021 taattatctt ttcgctatac tgccaaatat ggacatgatg aacatccttt accccatcga
    10081 ttgcttcaat ttcttgttcc attttttcat agtcgatagc aggtgcactt tgtatcaaaa
    10141 tagcggcagc ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca
    10201 gtgtaataac tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa
    10261 tcacacttac cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca
    10321 aactttcatc tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa
    10381 taactgctac tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga
    10441 atgcttcaac ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac
    10501 tcaatatttc taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca
    10561 ttttttgtgc cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat
    10621 ctgaaacaag agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca
    10681 ccgcattcaa aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca
    10741 ctattccttc ctccatttta tttatattgg atagtcttta gacaagaata acattttttt
    10801 gatatttcac caatcattca gctgtttcct taaagcactt gtatttcact tgatacttcg
    10861 ctagaattgc aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa
    10921 ttatcagttc ctttatacga ttcaaagacc taaaggagtc gagaaatgtc ccattttgga
    10981 aagccattgt ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag
    11041 tttttcaagt cttaaacaac attcaattgg atgggcaaaa gattgctact cgcattcctg
    11101 attttacaat ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa
    11161 caaactccat tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta
    11221 ctgatttgat ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc
    11281 ttcctaatac aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca
    11341 ccaatgaatc tttaaaaaac ttaacaggta agcagctccg ttctttttta agtgaagcat
    11401 ctataccact ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga
    11461 atctgctgat cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac
    11521 taacaaaagc tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta
    11581 ctataattgc aacaatcttg atggtctttc ttccttcttt tgacactagc gccttcattg
    11641 cttttgcagg aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca
    11701 attaagaaat aaagacaact cgttatttct acttcaatag acccttttag aggttgtgac
    11761 atttttttgt cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa
    11821 ggcgcttcaa agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa
    11881 ctatgtcaga aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa
    11941 tccatactat agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa
    12001 atgtagagat cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta
    12061 ccttgaaatg ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt
    12121 caaagaaaca tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc
    12181 atgtcttagg aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaggcacaga
    12241 gtgacatgaa atggttaggt tcatcggtcc attaccaatc tatccaaggg aaaaaaatac
    12301 taatcattgg aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca
    12361 tgaactgcta tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt
    12421 ctttaaatca actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc
    12481 taacggagga aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat
    12541 ctgtattcat caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt
    12601 taaataacca gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac
    12661 aagatcatcc tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga
    12721 cacctgattt tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg
    12781 ccacccatga gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa
    12841 caatattttc aaaagaaaaa agagaaggtg acaa
//
LOCUS       DASHFF010000074        12715 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.074, whole genome shotgun sequence.
ACCESSION   DASHFF010000074 DASHFF010000000
VERSION     DASHFF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.074"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..255
                     /locus_tag="U2I75_002320"
     CDS             <1..255
                     /locus_tag="U2I75_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697415.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="U2I75_002321"
                     /pseudo
     CDS             258..411
                     /locus_tag="U2I75_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="U2I75_002322"
     CDS             434..1639
                     /locus_tag="U2I75_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1697416.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            2423..4333
                     /gene="ltrA"
                     /locus_tag="U2I75_002323"
     CDS             2423..4333
                     /gene="ltrA"
                     /locus_tag="U2I75_002323"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1697417.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            4437..4661
                     /locus_tag="U2I75_002324"
     CDS             4437..4661
                     /locus_tag="U2I75_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697418.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4778..5275
                     /locus_tag="U2I75_002325"
     CDS             4778..5275
                     /locus_tag="U2I75_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1697419.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            5362..5754
                     /locus_tag="U2I75_002326"
     CDS             5362..5754
                     /locus_tag="U2I75_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1697420.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            5738..8185
                     /locus_tag="U2I75_002327"
     CDS             5738..8185
                     /locus_tag="U2I75_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1697421.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            8188..10371
                     /locus_tag="U2I75_002328"
     CDS             8188..10371
                     /locus_tag="U2I75_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1697422.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            10368..11369
                     /locus_tag="U2I75_002329"
     CDS             10368..11369
                     /locus_tag="U2I75_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN1697423.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            11384..12298
                     /locus_tag="U2I75_002330"
     CDS             11384..12298
                     /locus_tag="U2I75_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1697424.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            12543..12659
                     /locus_tag="U2I75_002331"
     CDS             12543..12659
                     /locus_tag="U2I75_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN1697425.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         4191 a         2320 c         2695 g         3509 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc tttgtgcgac tcgttcctta gtgaaaagct aaggcactaa
     1741 taaaagaacg aaaaagtttg gttattagga gaactgataa tctgacaggt ggaatgatga
     1801 agtaacgctc tgaaacgcct gccctgagcc tccgacaagc attgtatgag gaaactcaga
     1861 atgtttgaag ctcggtaaag acggctgaaa gataccgtaa tagttgatga acatatatct
     1921 tctatcgaaa actatccaga ggtgggtagc gtatccaaca ggtcgggggt ctataaaata
     1981 tctatggtta gaatgttcta taaggaactg acgaaacagc gaatgtacgg gtctaaattt
     2041 gaactttgta gaaatgcaaa ggtacgagaa atcgtagctg gtgtggcaaa gtaagaatat
     2101 aggttatgaa atgccatata acgttacagg cggtatcaag ccagcaggct tatagctagt
     2161 gacctaagga tatatatgta tagataagat tatcggaacg aggaaaggta ttgggttctc
     2221 atcaagagaa taaactgacg aagaacaata agcagttgaa ctaatgctga aaagcagagg
     2281 gcgaacctaa gaaattcttg taatgagaat ggaggaatgc cctcaagtcg tgtatatatt
     2341 agaaaagtat tatgttgttt caataaggat tacgagtaag ataaaaaagc tcactcaatt
     2401 tcaagtaaag gagtgaagcc ttatgcctaa aaataaaaat gaaacattgt gtgtagaaga
     2461 tttaagacac gctgaatatt acgaaatgca aaacaccttt gatgatttat atgccaaaag
     2521 taagaatgga gatattttca ctcatcttat ggatattatc ctatcgagag aaaatatatt
     2581 actggcatat cgaaatatca aagcaaatgc aggcagtaaa acagcaggaa cagacggtac
     2641 aataattaaa gatattggca aattgccagc agaaacagtt gtcaaaaaag taagatacat
     2701 tgttgcaggc agtccacatg ggtatcgacc taagccagtg aggcgaaaag aaataccaaa
     2761 accaaatggt aaaaccagac ctctaggtat tccttgtatg tgggacaggc ttatacaaca
     2821 atgtataaaa caagtattag aaccaatctg cgaagctaaa tttagtgaaa acagctatgg
     2881 ttttagacct aataggtcag ttgaaaatgc aataaaagca acctataacc gcctacaaat
     2941 cagccagcta cattatgtga ttgaatttga tattaagggt ttctttgaca acgtgaacca
     3001 ttccaagctg ataaaacaga tatgggctat gggaatacgg gacaagcact tgatattcat
     3061 tttaaaaagg atattaaaag ctccaatcaa aatgactaac ggtacaataa catatccaga
     3121 gaaaggaact ccgcaagggg gaataatatc cccactacta gcaaacattg ttcttaatga
     3181 gttagaccat tgggtagaaa gccaatggca ggaaaatcct gttacaaaaa actatgtggt
     3241 tcatatcaat aaaagcggta gcccatgtaa aagcaacgct tacaaagaaa tgaagaaaac
     3301 aaagttaaag gaaatgtata tggttcgtta tgcagatgat tttagagttt tctgccgcta
     3361 caaggaaagt gctgaaaaag ccaaaatagc aatcactcaa tggattgaac aacgattaaa
     3421 attggaagta tcacaagaaa aaacaagaat tgtaaatgtt agaaaaagat actccgactt
     3481 tcttggtttc aagattaaaa tgataccgag aagaaagaag ttagttgtta aatcacatat
     3541 aagtgataaa caattcaaaa accaaaaaga caagctggta tcacaggcaa agaaaattgc
     3601 ttctccctca aaagggaaaa cggaattaga agaaacaaga ctttataatt caatggtatt
     3661 aggtatgcaa aattattatc agattgcaac gaatgtaaat ctggattgta tgaaactgaa
     3721 tagagctgta atggctatct tcactaacag attaggtaca aacaaaaggg gcagattgag
     3781 aaaaacaggc agacaactta cgacaaagga ggcaacaaga tatggtaaat ctaaaatgct
     3841 tcggtatgtt gctggcgagg gcgaacctat tttccctgtt ggatatatcc aacacagaaa
     3901 accaatggca aaggtgtatt tagcgaattg ctacactcta gagggaagaa aatttatcca
     3961 caccaatcta agtgttgata aattactgat gtaccagtta atgaaattat ctttggaaaa
     4021 tcgcacaata gaatacgcag acaatcgaat ttcgctattt tctgctcaac atggaaaatg
     4081 tgctataact ctggaagaat ttcaacaagc taatgaaata cattgtcatc atattgtacc
     4141 aactggagct ggtggaaatg acgattataa aaatcttatt ttggtaaaag aggcggtaca
     4201 ccgtctaata cacgctaaaa cagaagaaac aatacaaaaa tatatagctc tattgaagct
     4261 aaataaagtc cagctcataa gactaaataa atatagagta ttagctggca atcaagaatt
     4321 aaacttgata taggtctact acataaatac ttttcaatat aaacacgatg gaacgcggag
     4381 tgcggtgaaa gtcgcacgct ccgtgtgaat ggggggaaaa gtttgagata tttataatat
     4441 caggaactta cctatccata tataactggt ttctatcaaa cgctcaatca ctggtgcttt
     4501 tagcaatcgt tgtgattggc ttgtatcttg gcttcaagcg tgagtttagc aaactgattg
     4561 gctttttaat tattgcgatt attgcggttg gcttagtctt caacgctgct ggagtaaaag
     4621 acattttact agagctattc aatcgcatta ttggtgctta aataaaaccg ttcttttgtg
     4681 gaatataagt ggttttctta tgttccgcaa aggaatggta caccaaacga agtgcggtag
     4741 ggatttttga atctctacaa agaaaggacg tgaatatatg gacgatatgc aagtctatat
     4801 tgcgaattta ggcaaataca atgagggcga attggtcggt gcgtggttta cctttcccat
     4861 tgactttgag gaagtcaaag agaaaatcgg cttgaatgat gaatatgagg aatacgccat
     4921 tcatgattac gagttaccct ttacggttga cgaatacact tctattagcg aactcaatcg
     4981 cctatgggaa atggtatcgg agttgccaga agaactacaa tcggagctat ctgctctgct
     5041 cactcatttt tcaagtattg aagaactaag cgaacatcaa gaagatatta tcattcattc
     5101 cgattgtgat gatatgtctg acgtggcacg ctactacatt gaagaaacgg gtgctttagg
     5161 cgaagtgcca gccagtcttc aaaactatat tgattatgaa tcctatggtc gggatttaga
     5221 cctttcggga acgtttatct caaccaatca cgggattttt gaaatcgtct attaaatctg
     5281 tcggtacatt cttactggca gattttcttg tttacggggt agtcaaaaga ctatccccat
     5341 ttttatgaaa ggattgatta catgaagaaa atacgaagct ataccagtat ctggtctgtt
     5401 gagaaagtgt tgtactccat caatgacttt agacttccgt ttcccataac ctttacgcaa
     5461 atgacatggt ttgtcgtgtc actctttgca gttatgatac ttggcaactt gccccctctt
     5521 tctatgatag agggagcatt tctcaaatac tttgggattc ctgtggcttt cacatggttt
     5581 atgtctacaa aaacctttga tggtaaaaag ccttatggat ttttgaagtc tgtcattgct
     5641 tatgcactgc gacgaaagct gacctatgca ggaaaaaaag taacgcttgg cagaaaccag
     5701 ccacaagaag ccattacagc agttaggagt gaattttatg gcatatccaa ttaaatatat
     5761 tgaaaacaat ctggtctgga ataaagatgg ggaatgttac gcttactatg agcttgttcc
     5821 ctacaattac tcatttctaa gtccggaaca gaaaatacaa gtacatgatt ctttcagaca
     5881 gcttatcgca caaaatcgtg atggcaagat tcatgcttta caaatcagta cagaatccag
     5941 catactttct gcacaagaac gttccaaaaa tgaagtcact ggaaagctca aaacggttgc
     6001 ctatgacaaa atcgaccaac agacagacgc tttaatatcc atgattggcg aaaatcaagt
     6061 ggactaccgt ttctttatcg gttttaagtt gcttctcaac gatcaggagt tttctatgaa
     6121 aagtcttacc gttgaagcaa aaaatgcttt tactgatttt gtctatgatg tgaaccataa
     6181 gctgatgggc gattttgtta gtatgagtaa tgatgaaatc ctgcgttttc agaagatgga
     6241 aaagctttta gaaaataaaa tctcccgtcg tttcaaaatc cgaagattag ataaggatga
     6301 cttcggctat ctgattgaac acctttacgg acagacaggc actgcctatg aagagtatga
     6361 gtatcctcta tcaaagaaaa agctggaaca tgaaacactg attaaaacct atgacctcat
     6421 taagcctact cgctgtctgg tggaagaaaa acagcgatat ttgaaaatcc agcaggaaga
     6481 cgaaaccgcc tatgtagctt actttaccat caacagtatt gtcggcgaac tggacttccc
     6541 gtcctctgaa atcttctact accagcaaca gcaatttacc tttccgattg atacgtcaat
     6601 gaatgtggaa attgtagcga atcgtaaagc cctatctact gtccgcaata aaaagaaaga
     6661 actgaaagac ttggataacc acgcttggca aagtgataat gaaaccagct ctaatgtggc
     6721 ggaagctctg gaaagtgtga acgagctgga aaccaattta gaccaaagca aggaatctat
     6781 gtataagctg tcctatgttg taagggtatc agcaaatgac cttgacgaac ttaaacgtcg
     6841 ttgtaatgaa gtgaaagatt tctatgatga tttgagcgtg aaactggtac gaccttttgg
     6901 ggatatgctg ggcttacatg aagaattttt acctgccagc aaaagatata tgaatgacta
     6961 tattcaatac gtgacctctg atttcctcgc tggtttaggt tttggtgcta ctcaaatgct
     7021 gggtgaaaat gaggggattt atgttggcta cagcttagat actggacgca atgtctatct
     7081 gaaacctgct cttgccagtc aaggggttaa gggttcagta accaatgcgt tagcgtctgc
     7141 ctttgtcggt tcgctgggtg gtggtaaatc ctttgcgaat aaccttatcg tctattatgc
     7201 agtgctttat ggggcacaag cagtgattgt agacccaaaa gcagaacgtg gcagatggaa
     7261 agaaaccttg ccagagattt cccatgaaat caatatcgtc aatctgactt ctgatgagaa
     7321 aaacaaaggc ttacttgacc cttatgtaat tatgaaaaat accaaagatt ctgaatcact
     7381 ggctattgat attttgacat tccttacggg gatttcctct cgtgatgggg aacgcttccc
     7441 aatccttaga aaagccattc gtgcagtaac caatagtgaa gtgcgagggt tgatgaaagt
     7501 gattgaagaa ttacgggttg agaatacgcc actaagtacc agcatagccg accatatcga
     7561 aagttttaca gactatgact ttgcccatct gctttttagt gatggttatg tagagcagtc
     7621 tatcagcctt gaaaaacaac tgaacattat acaggttgcc gacttggtac ttcctgacaa
     7681 ggaaacttcc tttgaggaat ataccacaat ggagttacta tcggtagcaa tgctgattgt
     7741 cattagtacc tttgcgttgg actttatcca tacagaccga agtattttca agattgtaga
     7801 cttagacgaa gcatggagct tcttacaggt ggcacaaggg aaaacactat ctatgaagct
     7861 ggttcgtgct ggtcgtgcta tgaacgctgg ggtatatttc gtgacccaaa atacagatga
     7921 cctcttagat gaaaaactga aaaataacct cggcttaaaa tttgcctttc gttccactga
     7981 ccttaacgag attaaaaaaa cccttgcctt ttttggtgta gacccagagg acgaaaacaa
     8041 tcagaagcga ttgcgtgatt tggaaaacgg gcaatgcctt atcagtgatt tatatggtcg
     8101 tgtcggtgtg atacagttcc accctgtatt tgaagaactg ctccatgcct ttgataccag
     8161 accacctgtg cgaaaagagg tgtaaatgtg aaaccatcaa tattaaacag aataaaatca
     8221 aactggacgc tgaaacgtct aggtaaagtg gcaatgacag tggctttcac acttgtgatt
     8281 gccatttttc ttttagccat gctgggaacg gtggttcaag ctgcgggctt ggtagatgat
     8341 acggtcaatg tggcaaatga atacagccga tacccacttg aaaactatca actggatttt
     8401 tatgtggata atagctgggg ctggctaccg tggaactggt cggacggaat tggaaaacaa
     8461 gtcatgtatg gactatatgc cattaccaat tttatttgga caatcagtct ttatgtttcc
     8521 aatgcgacgg gttacttagt acaggaagcc tattccttag acttcatttc cgctacagca
     8581 gattccattg gcaagaatat gcagaccctt gcaggggtta cgcctagtgg actatcaaca
     8641 gagggtttct atgttggatt ccttttactc ttgattttgg ttcttggggt ttatgttgcc
     8701 tatacaggac tgataaagag agaaaccaca aaggcaattc atgccctcat gaattttgtg
     8761 ctggttttta tcctatcggc ttcctttatt gcctacgctc ccgactacat taaaaaaatc
     8821 aatgactttt catcagacat cagcaacgct agtttgtcac tgggtacaaa aattgtcatg
     8881 ccccattcag atagtcaagg caaggacagt gtggacttga tacgtgacag cctattttcc
     8941 atacaggttc agcaaccgtg gctactgctt caatataaca gttcagacat tgaaagtatt
     9001 ggtattgacc gtgtggaaag cctgctctca accagtccag attccaacaa tggggaagac
     9061 agagaaaaaa ttgttgcgga agaaattgaa gatagaagca ataccaatat gacgattaca
     9121 aagacgatta accgtttagg tacagtcttc ttcctatttg tcttcaatat tgggatttcc
     9181 atatttgtat tcctattaac aggaatcatg attttctcgc aggtactttt tatcatctat
     9241 gctatgtttc tgcctgtgag ctttatttta agcatgattc catcatttga tggtatgtca
     9301 aaacgagcca taacaaagct ctttaatacc attttgacac gagctggaat cacattgatt
     9361 attacgacag catttagtat ttcaaccatg ctctatacct tatcggctgg ttatccgttc
     9421 tttttgattg cttttctaca gattgtgacc tttgcaggaa tctactttaa gctgggcgat
     9481 ttaatgagta tgttttctct acagagtaac gattctcaaa gtgtgggaag tcgtatgatg
     9541 agaaaacctc gtatgcttat gcacgctcac atgcaccgtc tacagcggaa acttggacgc
     9601 tccgtgactg ctttaggggc tgggtctgcc attgccagtg ctacgggtaa aaaaggacag
     9661 tcgggttcgg gaagttctgc gagtacacaa gcagatcact ctcgaccaaa cgggcaggaa
     9721 aaatcaacac taggaaagcg tatcggtcaa accatcggta cagtagctga taccaaagac
     9781 agaatggtag atactgctgg taatttgaaa gaacaggtta aggatttgcc gaccaatgca
     9841 agatatgcag tatatcaagg aaaatctaaa gtgacggata atgtccatga tttaaccaga
     9901 agtatttctc aaaccaaagc ggataaagcc agtggacgca agaaacagca ggaacaaaga
     9961 cggaaaacca ttgcggagcg tcgctctgaa atggaacagg tcaaacagaa aaaacagcca
    10021 gcttcttccg ttcatgaaag accagccact aagcaagaac aatctcatga tggacagact
    10081 tcaaggcaaa caactgtaca ggcttcctat agggaatctc aacaagccaa acaagagcgt
    10141 ccaacagtta agtccgattc ttcaagttta aaaacggaac gtcaaagtaa tacagttcaa
    10201 gaaagaacgg ttcaaaagcc agtaacttca actgcaccaa cagatagagc ttcacaacgt
    10261 tcaatcacaa aagaacgtca gtctactgtt caaagagtac cactacaaaa tacaaaaagt
    10321 agaccaccaa tcaaaaccgc caccattaag aaaggcagta agaaaccatg aagctgaaaa
    10381 ctttagtgat tggtggttcg ggattattct tgatggtctt ctcactgctt ctgtttgtcg
    10441 ccattttatt ttcagatgaa caagatggcg gtttttccaa cattcactat ggcggtgtgg
    10501 atgtttccgc agaagtgctg gctcataagc ctatggtaga aaaagttgcc aaagaatatg
    10561 gaattgaaga atacgtcaat atacttcttg cgattataca ggtggaatcg ggtggtacag
    10621 cagaagatgt tatgcagtcc tcggaatccc tcggtcttcc acctaattca ttgagtacag
    10681 aagaatccat taagcaaggt gtgaagtatt tcagtgagtt attagccagt agcgaaaggc
    10741 ttggtgtaga tttagaatcg gttatccagt cctacaatta tggtggtggt ttcttaggat
    10801 atgtggctaa tcgtgggaat aaatatacct ttgaactggc tcaaagtttc tcaaaagagt
    10861 attcgggtga cgaaaaagtg tcttacccca atcccatagc catacctatc aatgggggct
    10921 ggcgatacaa ttacgggaat atgttttatg tgcaactggt aacgcagtat cttgtcacaa
    10981 cagagtttga tgatgataca gtacaagcca tcatggacga agctctgaaa tttgaaggct
    11041 ggaagtatgt ctatggcgga gcttccccga ctacctcttt tgattgtagc ggactgacac
    11101 aatggacgta tggaaaagct ggaatcaact taccacgaac agcacaacag caatatgatg
    11161 taacccggca tatcccatta ttggaagcaa aagccggcga tttggttttc tttcattcta
    11221 cctataacgc tggctcttat attactcata tcgggattta tttaggcgat aaccgtatgt
    11281 ttcatgcagg cgaccctatc ggttatgccg acttaacaag ctcttactgg caacagcatt
    11341 tagtgggagc aggacgaatc aaacaataag aaaggacgat tcaatgatga aattcggaaa
    11401 aaatcagaat acagaaaaac agacaccaaa ggaaaagaaa cctcgtgtct ataaggtcaa
    11461 tcctcgtaaa aaggttgtga ttgtcctatg ggtactttta ggacttagtt tcagctttgc
    11521 gatattcaag cactttacag ccattgatac ccataccatt catgaaacca ccatcataga
    11581 aaaggaatat gtcgatactc atcatgtgga aaattttgta gagaactttg cgaaagtcta
    11641 ctattcatgg gaactaaacg acaagtccat tgataatcgc atagaaaacc tgaaagcata
    11701 tctgacagag gaacttcaag ctctgaatgt cgatacagtt cgtaaagata ttcctgtatc
    11761 gtcttctgta aaaggatttc agatatggac ggtagaagca gatggcgaca atcaatttga
    11821 tgtaacctac agtgtagacc aactcattac agagggggaa aatacaaaga ccgtccactc
    11881 tgcttatata gtgagtgtct atgtagacag tactggaaat atggtcctca ttaagaatcc
    11941 gaccattact agcataccaa agaaatcaga ctataaacca aaagctattg aaagtgaggg
    12001 tacggttgat tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct
    12061 ctatcctaca gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc
    12121 aatcggaaaa gagtacatct ttcaagaact ggtaaatcct atttacaatc gtaaggataa
    12181 tcaagtcacg gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt
    12241 atctcaattt gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca
    12301 aatattggta cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg
    12361 agattccttt acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt
    12421 aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagtgg gaacaaataa
    12481 ttggatgtcc ttttttagga gggcttagtt ttttgcaccc agtttaagaa tacctttatc
    12541 atgtgattct aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata
    12601 aaaatcccag tgataagagt atttatcact gggattttta tgcccttttg ggcttttgaa
    12661 tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt gatgc
//
LOCUS       DASHFF010000075        12109 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.075, whole genome shotgun sequence.
ACCESSION   DASHFF010000075 DASHFF010000000
VERSION     DASHFF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.075"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            51..260
                     /locus_tag="U2I75_002332"
     CDS             51..260
                     /locus_tag="U2I75_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697426.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="U2I75_002333"
                     /pseudo
     CDS             467..667
                     /locus_tag="U2I75_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="U2I75_002334"
     CDS             complement(1379..1984)
                     /locus_tag="U2I75_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1697427.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="U2I75_002335"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="U2I75_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="U2I75_002336"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="U2I75_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="U2I75_002337"
     CDS             3019..3990
                     /locus_tag="U2I75_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1697428.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="U2I75_002338"
     CDS             4130..5443
                     /locus_tag="U2I75_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1697429.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="U2I75_002339"
     CDS             5486..6352
                     /locus_tag="U2I75_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1697430.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="U2I75_002340"
     CDS             6349..7197
                     /locus_tag="U2I75_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1697431.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="U2I75_002341"
     CDS             7211..8683
                     /locus_tag="U2I75_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEN1697432.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="U2I75_002342"
     CDS             8700..9572
                     /locus_tag="U2I75_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1697433.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9808..11190)
                     /locus_tag="U2I75_002343"
                     /pseudo
     CDS             complement(9808..11190)
                     /locus_tag="U2I75_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11652..11933
                     /locus_tag="U2I75_002344"
                     /pseudo
     CDS             11652..11933
                     /locus_tag="U2I75_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            12007..>12109
                     /locus_tag="U2I75_002345"
     CDS             12007..>12109
                     /locus_tag="U2I75_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1697434.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
BASE COUNT         3765 a         2062 c         2436 g         3846 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aagttcttgc tcgaaagtta
     9721 tggaaatttc gatagccgta ggctacttgt attgtgcaat agttttttac aaaaaaaagg
     9781 gggtgcggat attttttttg gaccttttta acaaatatat ctttgcttct tccgatattg
     9841 aagagggctt agccttccta aagataactt aattcgttga ttattatacc aatgaatata
     9901 atcatccaat ttgtttataa actgattgat tgttgtattc ttccagtcac gttgataaaa
     9961 catctcgttt tttaatcgtc caaagaaacc ttcgcacgct gaattatctg gtgaataact
    10021 ctttttagac atagagtgaa gcaagttggc tttgttccaa tagtccaact aacaatagca
    10081 ccatcgtaac aatcgacaag tggtgataag tagattttcc ctgcgagaat tctaaattca
    10141 gtaatatctg ttaaccattt ttcatttggc ttctttgctt taaaattccg atttaataaa
    10201 ttggggtgtg caggagaaat ttccccagca tacgaagaat acttttttcg tctagaaaat
    10261 ttaacaaata gattttcctc agtcatcaat ctacgtacaa ctttttcaga aagcttttta
    10321 cccatatttt ttaaagcttg gtgaattcta cggtaaccat acgcacagaa gttatgattg
    10381 aaaatcgtga taattaattt gacgttcttt gtaatactta tctggtttct caagtgcttg
    10441 tttttgataa aaatagctgc ttttaggaaa ctggagctgt tgaagaagct ctttcaaggt
    10501 gaactgattt cgtaaggcat caatcaatat cgttttttct tgatttgata gacaaagaag
    10561 attgacgcct ttttcttttt ttagcagttc agccgctcct tcgagtaatg ctttttcgag
    10621 ctgtaattgg tgtacttgtt tttgtaagtc ttctacttcc tttaaaagta gctctttttg
    10681 tttttcctga ttttttaaag tcatacacaa tggaaaatcc tttccaagaa ccgtgttttt
    10741 ccattgatat agagtaactc tactaattcc tccagattcg gctaaatttt tcctattagt
    10801 ttgcggttta tagaattttt tcaacacagc ttctttttga tcttgtgtta actttaccgc
    10861 actttttcgg agttttctag ctcctggtgc aagttcttca caccgctgcc gcaaggactc
    10921 tctattagga taaccaagca tgcgaatggt tcgtgcataa cattgaccat attcaaaata
    10981 atgattcacc gctattcgtt tttgttcttc agaatacttt gatttttttc ggtgctcctg
    11041 atggagctct ccggattcca aatactcttt ataccatttc cgtagtgcac caatcgacgg
    11101 ataccctaat tctcgaatgg ttgcagcata agagcaatcg tattttaaaa ataactgaat
    11161 tgcccttaca cgctctttgt gggaaaacac acggacattc ctcctaagtt tattttttcg
    11221 tccaggatta tgtccgcacc cccttgcttt ctgatgactt attctggttc tttgagtgtc
    11281 aatgcataga tgtgatgtag ctagttagaa aaaaattttg aatactcttt ttttctttca
    11341 gactcctttt taagtaagat tttgaagtgt gagttacggg gtcagaaact taaaagtttt
    11401 tgatccttta ttttcaagtc tgatttggca gcttttcggc agctcattta tttatggtca
    11461 gaaatagatg ctgaaatttt aatctattag caaatagtcc agaacgtatg aagaggagtc
    11521 ggatatcatt tgtcattaac accataaata tttataaaat tcgtgttcac ctacaattaa
    11581 atttaatata taatattcgt tgtaagggct tataattttt tgttaatgaa caagatagga
    11641 agaaacattt aatggacagt tttcagagat tgtcactagt aaatcaattt gagttattgg
    11701 ctaaatttga ggaccaaaac agatcttatt atgaaagaaa aatcgaaata ttgagagaag
    11761 gttacgagta tcattatgat gatgaaattt ggagtgatct ttctgagcca tttccgaaag
    11821 aagattctcg atttgtatta gatgtattga acatgtatcg agatatcaat tttagtaaat
    11881 caaagctaaa cttagatgaa aaccaaatta ctaattcata ctatactcat ttcggttctg
    11941 ttgcaaagtt ttccaaaaaa tctattttag tgtaaaattg agaaaaaaga cagagaggac
    12001 agagtaatga atcattttaa aggcaaacaa ttcaaaaaag acgtcattat tgtcgctgtt
    12061 ggttactacc tgcgttacaa tctaagctat cgtgaagttc aggaattgt
//
LOCUS       DASHFF010000076        11838 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.076, whole genome shotgun sequence.
ACCESSION   DASHFF010000076 DASHFF010000000
VERSION     DASHFF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.076"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(560..1600)
                     /locus_tag="U2I75_002346"
     CDS             complement(560..1600)
                     /locus_tag="U2I75_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1697435.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="U2I75_002347"
     CDS             complement(1949..2275)
                     /locus_tag="U2I75_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697436.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3065)
                     /locus_tag="U2I75_002348"
     CDS             complement(2262..3065)
                     /locus_tag="U2I75_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1697437.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="U2I75_002349"
     CDS             complement(3418..3708)
                     /locus_tag="U2I75_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN1697438.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="U2I75_002350"
     CDS             complement(3844..4023)
                     /locus_tag="U2I75_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697439.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="U2I75_002351"
     CDS             complement(4020..4370)
                     /locus_tag="U2I75_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697440.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="U2I75_002352"
     CDS             complement(4363..5688)
                     /locus_tag="U2I75_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN1697441.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="U2I75_002353"
     CDS             6138..6752
                     /locus_tag="U2I75_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1697442.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..7159)
                     /locus_tag="U2I75_002354"
                     /pseudo
     CDS             complement(6917..7159)
                     /locus_tag="U2I75_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7363..8682)
                     /locus_tag="U2I75_002355"
     CDS             complement(7363..8682)
                     /locus_tag="U2I75_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HEN1697443.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(8837..9424)
                     /locus_tag="U2I75_002356"
                     /pseudo
     CDS             complement(8837..9424)
                     /locus_tag="U2I75_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10062..10319)
                     /locus_tag="U2I75_002357"
     CDS             complement(10062..10319)
                     /locus_tag="U2I75_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1697444.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10312..10581)
                     /locus_tag="U2I75_002358"
     CDS             complement(10312..10581)
                     /locus_tag="U2I75_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1697445.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(10712..10891)
                     /locus_tag="U2I75_002359"
     CDS             complement(10712..10891)
                     /locus_tag="U2I75_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697446.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(11272..>11838)
                     /locus_tag="U2I75_002360"
     CDS             complement(11272..>11838)
                     /locus_tag="U2I75_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697447.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3604 a         2163 c         1789 g         4282 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tattctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctatcc ctgaataatt catagcttta attctttggc acattttatt
     7201 gaaatatgta tcactaatat ccctatcaac tgattttgac taaaaagtta cttcaaacat
     7261 tcactgttca aaaatattga ggaataaatt ttgggtgtaa ggcattttga gcatacttct
     7321 ccctggataa cttcccagta aaaaaatcgt tagattgttg gattagatcc attgactggc
     7381 tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga tacacatgat aactagataa
     7441 tttgagatag actcgtctac ccgtttggac taatttccca gcaactttca gaaacttcaa
     7501 tcgaatagaa tgaatggtca ttcccttttg gacttcttca aagccaatcg ttcttaagaa
     7561 gttgactaag ttgtaagcta tcaagctgag catcattcgg acatgattct ccaaaaagcg
     7621 aggactgtct gttttgtcaa agtaaaagcc agctttcgct tctttaatga agttctccat
     7681 tgtgccacgt ttggcataga tagaaaagat ggtatcagga gaaacatttt ctgatagatt
     7741 cgtcacgata aattcatgtc gaaagagtag ttcgcccgct tcacgtgttg agcgtataca
     7801 cacgcgacga ctttgtgacc aggattgtgc ttgataagtg gtcgagaagt actgaacttc
     7861 tcgttcttcc cacttttgat tatcgccata aagtactgat ttctcagcta tttgacctag
     7921 tctacgatta ttcttcagtc gaataacata atgactcttt tttgattcac acgaatcata
     7981 cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga
     8041 gtgattatag tgctctaata aaggtgtgag aaactccttt acgccttttg atgtatattg
     8101 atttcctgaa cgtagttcag cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa
     8161 tggatggtaa ccataggttt ggtagtgtgt attataattc gtttgttctt ggtgaccaaa
     8221 tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc gtatcattac ggatgaggcg
     8281 tgctttatcg ataagttctt ggttcaaagc ttgaagttca tggatattct cctcagatag
     8341 tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt tctttcctat ctaaaacagc
     8401 tttaaacaca ggatcttgtc tcaggagatt agctgcagaa tcagctgagt aaccagcaat
     8461 taattgcata atgaactgct caagtatgga taagttgtca tgagtaaaat acgctcgttc
     8521 gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc
     8581 tttcactagg acgagacccg aatcactcga taattgacca cctgtatgag aaatagtgat
     8641 atttgaattg aattttactt ggtttttgtg taagctagtc atgaaaagaa ctcctttctt
     8701 ttggttggtt tagtcacttt aaccatagca gaaaggggtt cttttttcat cacttaacgg
     8761 gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa
     8821 agtatgaatt attcaggcta tcattaattt tataaaaaaa attaaatgtt tttagcactg
     8881 ccatataaca ttcaaaacat tcttctatag ttggtttact tccttgagga gaatgaacca
     8941 atttattacg tttttctatg aaatttttaa tttcctttct tatttctttc ggaaactcaa
     9001 tattaaagta ccctttaaac atgctcccta ataatttgtc tacaggggga gattgtaaat
     9061 tctcaaatat tagagttaaa tcaggtttct cttgttgata aaattccttt acccctattt
     9121 cacaggctgt aattatcgaa ataaacctat ctctagtatt tgatatattt ttacttcttt
     9181 taaggagtat aagagaaaag gggatacttt cgtgtccatg catgtaatta actaattgtt
     9241 gagcttcaga agttatatct ctaattatat agttaacgga attaggaagt gtctttaact
     9301 ctaatgaaat ttctatccaa ttactatctt ctaatctgta tatgctcgaa gtataatttg
     9361 attcgtcagc aagcatcgct acatcccaaa cactgatcat aaaatctttt aaacgttcaa
     9421 tcatttcagc aatattgcta aaatcagcta atttttcttt agaataaaaa aaaacattta
     9481 tatcagaatc aataaattct atttttttat tactatatcg tcatcattat cttgaccatt
     9541 tatgtttatt gttccctgag tggggagtac ttctttcaaa ttcaagttaa atttatattc
     9601 cattacttct cctccaatat ataaaaatta cttaactgta tccaaattta atatgtaaat
     9661 aaatcattaa tttttagcta aattctatga gaagtttatt tttgttgttt ttgctaatta
     9721 actaaaacta gtacaaaaaa tttaaaatag attattaaac ctattgctta ttctattcag
     9781 tcatatatat aagcgtcatc ctttagtaac taacatccca cactacaata gtattataac
     9841 atatctattc aacttcaaat tgttgaaatt taagccattt atatttactt ttcctcaact
     9901 ttagaaatga ttttactaac cttatatccg taatacgcgt aacaaacaca ttcattaata
     9961 acaatcaaca ctaagctata ttagcttttt aaatgactag ggtaaataaa ataaggatca
    10021 aaaactttta agtttctgac cctttcctta cttccgattg gttaatagtg atcttttgca
    10081 gaataaataa atatcgtttc attttcaact ctatatatca gtctatgttc atctgtaatt
    10141 cttctggacc attttccaga taaatcatgc tttaatggct caggttttcc taatccagca
    10201 aagggggaac gatcgatatc ttttattaac ttgttaatct tttttatatt gcttttgttt
    10261 ccttgctcat gccaataaag ataatcatcc caagcatcat cagaccaagc cttaatcatc
    10321 agattcaacc tcgattaagt catgtgtttt aaatgcacct ttggagaatt gttcatctcc
    10381 tcgacgaatt ttttccatga cgtaattatt agaaagtgtt ctcaacgttt cttgcataga
    10441 atcataatct ctttttgata atacaacaac tgtatcttct acatctttac ttgttacaat
    10501 aagtgtttca gcatcctcat taacttgttt catataacta cgtaaatttt ggcggaaatt
    10561 tgaataagct actgcttcca ttcctttcac cccttattta tttctataaa acaattgtac
    10621 attatattgt acaattaagc aaatgattaa tattctaact tcttataatt aaaagtgcta
    10681 tcacttttag tttttaggat aaaaagtgac gtcacttttt gagtgctggt acctcaaatc
    10741 ctgctttttc ttgggcaatc aataaatcta acgcaataca cataatcctg gattcacttt
    10801 tgccgtactt ctgctttagt tgctgcaatt ctttttgtct cttttcatcc aacgtaacgg
    10861 gtaaccgttt atttcctatt gcaattggca ttagtactct ctcctttcat cgaaataacc
    10921 ctatgaaaaa tatggctgca atcatcttga atagtagact cacaagggct aatacaaagc
    10981 gccaaaagat caaacaaaat accagtattg ccaaaaatac tacgaagttt aaatgcacag
    11041 ctgcagcatg gcgcataagt atttcgccaa caaataggac taacaataga ggaatgattt
    11101 tattcatatg gatctccttc tttctcttag gttatcgggt gttgcccgct tatttcgcac
    11161 cacctaagcg gcgggggcgg ttggagcgat tggtgcgaaa taagaaccat tcccttagtt
    11221 actaggtttt ttgtccttat ttcacacatt ctcacttttc ggtataaatg attattgcct
    11281 ttttttcctt tcaaatcgtt gatatggcat tcgtttacgg tcaagttcat tttggatttc
    11341 ttctgtatcg tcgtaagtat aggtgtctaa aaaatcagtt aaagtcgtgt accagtcgtc
    11401 gcctgggtta tctacttctt ctacatattc cctcaaaaat gcttcgggta cagcgagttc
    11461 ataatccatt cccattaaca cgatagattc cgttccacac gtcaaaaatc tagtaaatac
    11521 aaggtaataa tcgtctatcg tcgcatcttt gctgtttagc tcttccattt cttcttccat
    11581 taattccatg ctctcaaaaa ttgaataata ttctgattct gagtaatagt gtttcctgca
    11641 aatcgtgtag ctgacaaagt tttcgaccag ctcactcctt tctaggtaaa cattcatgat
    11701 gtatttttct gtttctagtc gtagaagctc cgttcctttt tttaccgttt caggacgacg
    11761 tttatacacg tctaaacgat tatggctgac tgtgaccttc gtttgactcg tttcactttc
    11821 caaatacaat tgttcttc
//
LOCUS       DASHFF010000077        11588 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.077, whole genome shotgun sequence.
ACCESSION   DASHFF010000077 DASHFF010000000
VERSION     DASHFF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.077"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(48..2123)
                     /locus_tag="U2I75_002361"
     CDS             complement(48..2123)
                     /locus_tag="U2I75_002361"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN1697448.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2125..3042)
                     /gene="glyQ"
                     /locus_tag="U2I75_002362"
     CDS             complement(2125..3042)
                     /gene="glyQ"
                     /locus_tag="U2I75_002362"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN1697449.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3428..4240)
                     /gene="recO"
                     /locus_tag="U2I75_002363"
     CDS             complement(3428..4240)
                     /gene="recO"
                     /locus_tag="U2I75_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN1697450.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(4383..5282)
                     /gene="era"
                     /locus_tag="U2I75_002364"
     CDS             complement(4383..5282)
                     /gene="era"
                     /locus_tag="U2I75_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN1697451.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5297..5692)
                     /locus_tag="U2I75_002365"
     CDS             complement(5297..5692)
                     /locus_tag="U2I75_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN1697452.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(5676..6152)
                     /gene="ybeY"
                     /locus_tag="U2I75_002366"
     CDS             complement(5676..6152)
                     /gene="ybeY"
                     /locus_tag="U2I75_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN1697453.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6169..8361)
                     /locus_tag="U2I75_002367"
     CDS             complement(6169..8361)
                     /locus_tag="U2I75_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN1697454.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8385..9356)
                     /locus_tag="U2I75_002368"
     CDS             complement(8385..9356)
                     /locus_tag="U2I75_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN1697455.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10062..11441
                     /gene="brnQ"
                     /locus_tag="U2I75_002369"
     CDS             10062..11441
                     /gene="brnQ"
                     /locus_tag="U2I75_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1697456.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3128 a         2491 c         2142 g         3827 t
ORIGIN      
        1 gagacacagc acgtttcgtg catgctctct gtttttcaaa caaaagacta ttttgtgatg
       61 atttgatcca agcttgctaa tgacaaagcc atggcagcta tttgactaag ttgtgttagt
      121 cggttcttac gtactgctgg ttcatcagcc atgaccatcg tttgatcaaa ataattttcg
      181 atcagtggac gaagagcgac aagctggtcg tagttttcgt ggatcgtttg gtcagcaaaa
      241 cttgctttca ttgcttctac tgctttgtgt aattctttct ctgcgtcgtt ttcgaacagc
      301 tcaggatcta cttctgattg tttttcaagc tgcgcttttt tcgctaagtt cacgacacgt
      361 gtcaaagctt ccattgaagg tttgaagtct tcatctgcaa gatgttgttt gaacaattgt
      421 gctgctgcaa atacttttgt cagatctttt tgttccgcat tcaaaacagc atcgatgaca
      481 tcatgacgaa tattcttcgt tgtcaataat tggcgcagac ggcctttgat aaagtcgatc
      541 acttgttgtt gcccttctga taaaccaatg ccgaaacgat ccgtatcttt actgataaca
      601 tcgctaatcg tttcttgcag aacagacaaa ggaaaggccc attttttcga ttcgatgatc
      661 cgaacgacac cgtatgcttg gcgacgtaag gcataaggat cgtttgaacc gcttgggatc
      721 atcccaacag caaagaaaga aaagatgcta tctaatttat ctgcaatcgc taaaacagaa
      781 cctactgtag actgaggtag ctctccttca ctggatatag gcatgtaatg ctcacgaatc
      841 gctgtcgcta cagcaggttt ctcgcctttc agcagagcat atttttcgcc catgatccct
      901 tgcagttcag gaaattcccc taccatgttg gttactagat caaatttata gatttcagca
      961 gctcgttgca gatccatcaa ttcttcctca gatagtccta cttgttcgcc aatgatacgg
     1021 gcaatgactt gtacccgttg cattttctca tagatactac cgattttttc atggaaagta
     1081 acagttttta gtttttcaac gtattggtcg attgaagctt tcttgtcttc attataaaag
     1141 aattccgcat cttctaaacg agccgtcaat actttttgat tgcctcgaac cacattttcc
     1201 aattttaccc gatcaccatt acgagcagaa ataaaatgag gaagcagcat accttgatca
     1261 ttccgtacct cgaaataacg ttgatgttct ttcatagaag tgactagtac ttcttcagga
     1321 acagaaagat atttttcttc gaagtcgcca gaaaagacag ttggatattc gactaaattg
     1381 ttgacttctt ctagtaaatc ttcgtctaga tcaataaccc aatgattttt ttcggctaat
     1441 tcattggctt gtgtaacgat catttcttgt cttttttgcg gagacgcaat gacgaattgt
     1501 tcattcaatt tttcttcata ttcttttgca tgcgcaaatg tcacatcttc gcctaagaaa
     1561 cgatgacctc gagaggctct gcctgttttg acatccaaga tttcgaatgg gattacttcg
     1621 tcatctaaca atgccacgat ccagtgaata gggcggatat attcaaaatc gtattctgcc
     1681 caatgcatcg ttacagggaa agtcaggctt ttaatgacat ctgctaaacc tgtcaatact
     1741 tctttggatt ccttaccatg agtgtatttc gttacataga catattccac accttttaat
     1801 tctctaaaag tgatagcgtc tgtcgtcaga ccttgtccgc ggacaaatcc ttccgctgct
     1861 ttcgtccagt ttccatctgc gtccaaagca atttttttgg ctggtccttt gacttcttct
     1921 tcactgtccg cttgtcgttc tggaatgttt gttactttga ttgctaaacg gcgcggtgtg
     1981 gaaaatgttt cgatcgtatc ataagtcaat tggttttcat ctaaaaattt tgctgttttt
     2041 tcttctaatt gtttcatgct tggtgtgaca acatgagcag gcatttcttc cagcccgatt
     2101 tcaagtaata agtcttttgc catcttattc agcctccttc acgttttctg gatttgctgt
     2161 gtttttattc aacaaaggga atcctaattt ttcgcgttct gccacaaaga ttttggctac
     2221 agcacgagcc atgttgcgga tgcgagaaag ataaccggca cgctctgtta cggatactgc
     2281 accgcgggcg tctaataagt tgaatgtatg gctacatttc aagatataat catacgccgg
     2341 atgaacaaga ttttcatcga tacagcgttt agcttctttt tcaaaacgat caaagttgct
     2401 tagtagcaag tcttggtcgc ttacttcaaa agaatattta gaatgttcat attctggttg
     2461 tttgaagatc tcgccatatt tcacgccatc agcccattct aaatcataga cactttcaac
     2521 ttcttggata taagaagcaa gacgttctaa tccataagtg atctcagaag ttactggttt
     2581 acaaggcagt ccaccaactt gttggaagta cgtgaattgt gtgatttcca ttccgtcaag
     2641 ccatacttcc caacccacac cggcacagcc catggatggg ttctcccagt tgtcttcaac
     2701 aaaacggatg tcgtgttcta gtggatcgat tcctaaaagg cgcaagcttt ctaaataaag
     2761 ctcttggata ttctcaggtg aaggtttcat cacgacttgg aattgatgat gctgatacaa
     2821 acggtttggg ttttcgccat aacgtccatc agctggtcgg cgagatggct ctacgtaagc
     2881 agcattccat ggttctggtc cgattgcccg taaaaaggta tatgggctca tcgtacctgc
     2941 tcctttttct gtgtcatacg cttgcatcag catgcagccg tttgatgacc aaaatttttg
     3001 taatgttaaa atcatatctt ggacggtcaa ttttttactc atctgttttg cccctttcaa
     3061 ttttaaagcg ttgctgtcaa ataagaatac aggaaagaaa acgacaaaaa atccctatgt
     3121 ctgattacag acatagggac gaataattcg cggttccacc ctacttccga ataaagattc
     3181 ggcagcttca tttatcgtac tcgcaagtgc ctcttcaaaa atcgtttggg tccggctccc
     3241 actctcccga actcgctaaa ccacgtgttt tttactgatc ttggtcaatg tacgtattca
     3301 ttttgcagtt ttcttgtttc tataatagat cctctcagtg aacgatctat ttgatctacg
     3361 gttaataata gacggaactc cctgtgtttg tcaaatggtt ttgatttttt gttgtatgtt
     3421 tcatttacta ttcttctttt ttaggtagat ctttttcaga catgtctttc tctgacgtct
     3481 ttaatacgtg tgcccaagac ttcatttgat caatgaactt tttgcttttc aaatggatac
     3541 cgacatattc atcatacagc tggtcgatga gttcacggat tgcttgtttg gtttcttctt
     3601 tcagatggat tccactgatt ttatcataag aaatagctga aaacatccga acgaaataaa
     3661 cagctcgagg atctgcatga taacggtgat catccatcga ccagtgtctt tcgcatatca
     3721 caccaccata tttggaagaa tagtcaaatt ttccttttgt ttccccacat acgacgcagt
     3781 gtgtccagat tggtgcaatt ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga
     3841 tgatttctgc atcatctcct tggtccatca gttgcagtgc ttgttgcgta aactggaaca
     3901 aatgcgggtc gtactgatga tcttcgattg ccgcatctac tagattcaat aaatatgtgc
     3961 catagccgct caaaaagatg tcttcttgga tgtgtcgaaa cgcgtggaca tctttgacac
     4021 tgcttaaaaa tgacaaacct tcacttcgaa aattcccgat ataaactgct tcagtaaaag
     4081 ggagcaaagc cggagctaag ggctgatttt gccgatggat ccctttgata taaaacatca
     4141 ttttgccaga agattccgta aagattttga ctaatttatc tttttctttg taattacggc
     4201 tagataaaat caagcctttt gattctgcaa gctggctcat attctccctc ctaaaaaata
     4261 aatctggaaa agcagattct gccgatccag atcatttatg ttgcgatctt ccgcagcttt
     4321 tctcaacacg cggattttta tccagtttat ttctatacct aacttacaag aagctcagaa
     4381 tatcaatatt cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag
     4441 tctttttgaa ctttgaccca aagttctaga aagactttgt ctcctaaaag attttcgata
     4501 tcttttctcg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatc
     4561 cctttttggc tatctctttc gacaataatc gtagcctgga tatggatttt atcattctcg
     4621 tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa
     4681 aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact
     4741 tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac atccatcaaa
     4801 cgttcgacgt tgtttccttc tgtagcagaa attggaatca cttgtgcaaa cggcatctgc
     4861 gttgaataat cttcgatgat tcccaacaac tcgtctggat ggattttatc gattttattg
     4921 ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc
     4981 cgaccgcgtt tttgatcagc gctgatcata aataagaccg cgtccacttc tttcaacgca
     5041 ctgtaagctg cttctaccat aaaatctcct aaacgatgct ttggtttatg gattcctggt
     5101 gtgtcaataa aaatcaactg agcttctggt gtagtatata ctccttggat tttatttcta
     5161 gttgtctgcg ccttatcact catgatggca attttttgac ccacgattcg attcaataat
     5221 gtggattttc ctacattcgg gcggccgacg attgcgacaa accctgattt atgttctggc
     5281 atatacgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca
     5341 acccaacgat gacggaaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga
     5401 tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa
     5461 cagtattgat gatctctact atccatacga gaaaaacagc taaaagcagc cataaccatt
     5521 ctatccgatc aagctggaaa atcacgccca taaacaaagc gaggattccg aacgcaacat
     5581 gtttcctcat gttgcgttct tcctcaaaaa cggttttgac tccttggacg gcaaattcca
     5641 cggacatcag gaaatgtttg tttttctcta cgtttttatc ttttaagtcc ataggcatct
     5701 aagatctctt tctgtaaacc aaacatttct ttctcatcct ctggtgtcat atggtcatac
     5761 ccgttgatat gcaagaatcc atgaaccgct aaaaagccaa gttcccgatc atacgaatgg
     5821 ccgtaagctt ctgcttgctc tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca
     5881 cgaggcaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc gccctcttct
     5941 tccaaggcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt
     6001 acttggatcg ctgcattgtc catgaaagta acagacattt ctgtttcttc tggcaactct
     6061 aaatatgatg ctgcaaattg cagcaagtcg tcgatttctt tcattttctc ttctgagact
     6121 tggtttgttt catccatgaa agtaatatcc attatacatc gcttctttct attcttcttg
     6181 ttcttctttt atcttctcaa cttccgattt cattttagga tatttgatcc gagaatggaa
     6241 ggtactgctt aaaccgctga tcaatgattt ttctacttta cggatttctt ttaacgtcaa
     6301 tccactgtcg tccaattgtc catcggaaat acgctcttca ataagattat ggacaaaact
     6361 ttcgattttt tcgctcgttg gatggtccat tgctcgtact gccgcttcac aactgtcagc
     6421 tatattgacg attcctgctt cacggctttg aggttttggt ccaggataac ggaattgctc
     6481 ttccgtgact tccgggttgc gttcctttgc cttaacgtag aaataacgca tcagcgtggt
     6541 tccatgatgc tgtctgcaaa tatcgatcac catttgcggc atattataat cttccaatat
     6601 tttggcacca tctgtcacat gtccaaagat aatatgttta ctgtcttctg gcaataagaa
     6661 attgtgcgga ttttccgctc ctgaaggcaa gttctcaacg aaaaagctgg cgtgtttgat
     6721 cttgccgata tcatgataat agcaagctac tcgagttagt aaagaacgtc caccgatttc
     6781 tgctacagca ttagcactta gattggctac catcatgcta tggtgatacg tgcctggtgc
     6841 ttcttccagc aactgtttca ataaaggatg attaggattg ctgagttcat ttaatacgat
     6901 cacactatca tcattgacca tcaattcaat ataggggtgc agacctatcg tcagcaagaa
     6961 tgagaacagt gcacctgtga atccgcatac tagcgttaac caagtcgttg catccccaaa
     7021 gctcattcct tggtagataa tcaaaatgac atccatcatc acaggaaaaa taatgaccca
     7081 catagcagcg gataaccctt gttctgagat ccgtttgcgt ctgacgatcg ttgccatgag
     7141 accagaaaat acataggaca tcaaaataac tgtcagaaag ttcgtgccaa tcgaatcata
     7201 aaaaatgaaa agtgcggata ctgcttgaaa aataccagcc atgatccctg ctcttcgatt
     7261 caagaaaaag cttaaaacaa gtggagcaaa agccgcgggg taaaacagcg gaatataagc
     7321 agcttgttct gtttggaaga gttggaaaaa tttcattagt acgacgctta ctgacattgc
     7381 cgtcacgtag aaaagtacgt attttgttct ttctcctgca ttttggaatt gaagtgaatt
     7441 ataaatcagt acaccgattt gaagcaaaac agctaaaacc atggcaatga gtgggaaaat
     7501 agaagtcgtt tgattcgtca atcctagtaa ttccaatttt ttcatcgctg aagcatcaat
     7561 ttgatttcct tcgcgaacaa taatttctcc ttggaagatc atcactggtt gtacagcttc
     7621 tcgtgcagat tgttttaatt cttctgtctt tttctcattc aatacatcat taactacaat
     7681 gccttgatcc actaagtagc gaatcatttg ttgctgctct gttgtgacat tcaaatattg
     7741 aatctgatct tctgcttctt gtttaaaagc ttccaattcg ctttcacgaa cttgtttact
     7801 catctgttcg tcaatgagct tcaaactttc atccctaacc gtgtgaattt ccgtttcagt
     7861 catttcaaat atcgtcgaat aaaaattgtt cggcagtttt tggtaaaacg caacgttttc
     7921 tgcattcacg ttttcaaaat ttttcttcag tgtagcaatc cgctcttctg tagtaggttt
     7981 cgggatattt tcatcttcct tcgccttatc taccttgtca ttataagatt tgttgatggt
     8041 atcattcgtc ttatcgatca attcgaataa ttgcttgatc cgattgttct ggtcatctgc
     8101 cagatctttt tgataagtat attctggagt cactgcttct gctgccagtt ttcttctttg
     8161 ttcggtttct tccgtgttct caatcgtttt attcgcacgg atactctctt ctgctacttg
     8221 gccttctctg aaatcattgg ccttttgttt gacactacta aatgtaacgc cgcagacaat
     8281 gataaaaaac aagaacagaa ggaaaggcaa tgctgccttt ccgagttttt tttgtagttt
     8341 ctgcagtgtt ttattcaaca tgtgcttatc ctcctaagca aagcttattc ttgatgaaga
     8401 tccgcctttt cataagcttt gatgatttcg gcaactacag ggtgccgaac aacgtcactt
     8461 gcttcaaaat taacaaaatc gatttttttg atttgcttta atgttcgttc tgcatgaaca
     8521 agaccacttg tcgtcccacg aggcaagtct atctgacttg tatccccatt gacaatcatt
     8581 tttgattgat aaccgagacg ggtcaagaac attttcatct gagcgactgt cgtgttttgt
     8641 gcttcgtcca agataacgaa tgcatcatcc aatgtccgcc cccgcatgta agctaaaggt
     8701 gcgatttcga tcacgcctcg ttccatcaat cggttcgtat gatccaatcc aaaaatttgg
     8761 tataaagcat cataaacagg ccgcagataa ggatccactt tttctttcag atccccgggt
     8821 agaaagccta ggttttcccc tgcctccaca gcgggccgcg tcaaaatgat tttttgcact
     8881 tcgccttttt ttagggcagc aattgctaat actacggcaa gaaacgtttt ccctgttccg
     8941 gctggcccta caccaaatac gatgtcatgt ttggctaccg attcaacgta tttcttttga
     9001 ccgctgtttt ttacccgtat aggttttcca ttacggtctt tgacgatctc ttcctcatac
     9061 atctcaacaa aataatctaa tgtacctttt tgccccatct tcaatgccgt cacaacatct
     9121 ggcgtactta cgtggattcc tcgtttgatc aattcctgta atgtccgaat gactgaggca
     9181 gccaaggaaa tctgggattg ctcaccaatg atctgaatga tttctccacg tgtatggatg
     9241 gttgtttccg ttgtttcctc gatcagtttt atatgcttgt cgtgtgcacc taaaagcatg
     9301 ctgacatcat cagcagctgt caatctaatc tctaaagaac tagactcttc cgtcaaaaat
     9361 agacatcccc tttttttcga ttcattcttt cctattttac catactcaga agaaactata
     9421 tagcaaataa gaaaaggttg taaaagagtc gtttaaccat acgtaccaag aaacgttaaa
     9481 aaatagccgt ttccattcgc taaaggtctc gttttgataa aaagaagctg agaatgtaag
     9541 ttatatgaaa gtggagaaca aaatattccg tttcattcag caggtattct ctaggtattt
     9601 ttcttgtttc aaaaaaagaa aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca
     9661 ggtgtattat tacgtcaaca aataaacata cagcaaagcg tcgaggaaaa cagtaggcta
     9721 ttcaacgatt tattcccagt gagtcatgta agtgagaaat gacaataaat cgaatagacg
     9781 aacatcattt ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc cattatcagg
     9841 ctagcggata actttatcaa aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa
     9901 ggtggtaccg ttctagaatc tagagccctt ttcccaagtg gaaaagggct ttttttatat
     9961 tttaaggtgg tggttttcat ggaatctgat attagtggca ttactaattg ctgcaacgtt
    10021 tatatcgtca atcaaaatga taattattgg aggaaaaatt tatggaagaa acaaaaactt
    10081 attcatggaa acaactattg atggtcagtt cacttatctt cgggatgttc ttcgggtcag
    10141 gtaatctaat ctttcctgtc cacttaggtc aaatggctgg cgcaaactgg gtcagtgctg
    10201 gcgtaggatt tgccatctcg ggcgctcttt tcccattact agctatctta gctgtagtag
    10261 tgacaaaaag tgatggtctg tatgacttag ctaaacctgt cggaaaatgg tatgctgcct
    10321 tgttcttaat ccttgttcat ttgacgatcg gaccattttt cggtacacca cggacagctg
    10381 ctactgcttt tgaaatggct gctaaaccat ttttgcctga aaaatataat tcattaggta
    10441 tgtttttatt ctctgctctt ttctttttac ttgcttattt ccttactgtc catcccacac
    10501 ggttggtcaa atacgtcgga aagtatctaa actccttgtt tcttgcattg ttagcaatca
    10561 tttttgtcgt ttctttattc cacccaatgg gaaatctaaa tcaatctgct gcagatactt
    10621 accaaacaaa cgctttaaca aatggtgtcc tagaaggtta taacacggtt gatgcagtag
    10681 ccttgcttgc cttaagtgtc acttttgtcc atgcagtccg tgaccttggt ttcaaagata
    10741 aaaaagtcac agaattaaca gcaaaagctg gtagcctgag cgttgcgcta gaagtcatga
    10801 tctattttgg tcttgttctt ctcggtgcga tgagtttgaa taagatgtct ttatcagaaa
    10861 atggcggaac tgcactttct cagatcacta atttttattt aggtaacttc ggcgctagct
    10921 tcttaggagt gatggtcaca ttaggtgtct tcactaccgc tatgggactc gttgtttctt
    10981 ttgcgcaaga tttccataaa ctgttcccta aagtcagtta tatgacatgg ctgcggttaa
    11041 cgactttcgt ctcttttgtc gttgcaaatg ctggtttaga caatatcatc caatggtctt
    11101 taccagtatt gatgcttttg tatccattgt cgcttgcgtt gatcttgctt tccttaacag
    11161 caaaattttt ccaaaagaca ccatttgtct atcaagtaac gatgttgttt gctgctgttc
    11221 ctgccgtatt ggatatgctg gcaaattcgc ctgctttagt gagtcaacaa agagttgtag
    11281 caagcatgct cgaattttat catcatcacg ttccttttgc tgcattagga cttggctgga
    11341 tggtcccaac gttacttggt tacgctggca gcctactgtt ttattacgcc tatcgcctat
    11401 caggatataa acaagaagca aatgaactac cagaagaata accaaatcaa acacaattta
    11461 acataagaaa tattaaacag gaaaaaacca gctagatgaa agaatcctat tctttcgtct
    11521 agctattttt ttgttctctt ttttacgcgc acaaaaaaac tgcggacaat gattgtccgc
    11581 agtttaaa
//
LOCUS       DASHFF010000078        11572 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.078, whole genome shotgun sequence.
ACCESSION   DASHFF010000078 DASHFF010000000
VERSION     DASHFF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.078"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            115..621
                     /locus_tag="U2I75_002370"
     CDS             115..621
                     /locus_tag="U2I75_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN1697457.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            638..1537
                     /locus_tag="U2I75_002371"
     CDS             638..1537
                     /locus_tag="U2I75_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN1697458.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1624..2508
                     /locus_tag="U2I75_002372"
     CDS             1624..2508
                     /locus_tag="U2I75_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1697459.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2700..2771)
                     /locus_tag="U2I75_002373"
     tRNA            complement(2700..2771)
                     /locus_tag="U2I75_002373"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2736..2738),aa:Arg,seq:ccg)
     gene            2831..4165
                     /gene="rpoN"
                     /locus_tag="U2I75_002374"
     CDS             2831..4165
                     /gene="rpoN"
                     /locus_tag="U2I75_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN1697460.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4314..5405
                     /locus_tag="U2I75_002375"
     CDS             4314..5405
                     /locus_tag="U2I75_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN1697461.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5446..6447
                     /gene="gap"
                     /locus_tag="U2I75_002376"
     CDS             5446..6447
                     /gene="gap"
                     /locus_tag="U2I75_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1697462.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6557..7747
                     /locus_tag="U2I75_002377"
     CDS             6557..7747
                     /locus_tag="U2I75_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN1697463.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7851..8606
                     /locus_tag="U2I75_002378"
     CDS             7851..8606
                     /locus_tag="U2I75_002378"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN1697464.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8722..10020
                     /gene="eno"
                     /locus_tag="U2I75_002379"
     CDS             8722..10020
                     /gene="eno"
                     /locus_tag="U2I75_002379"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN1697465.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            10419..11558
                     /locus_tag="U2I75_002380"
     CDS             10419..11558
                     /locus_tag="U2I75_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN1697466.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4039 a         2135 c         2412 g         2986 t
ORIGIN      
        1 tcacaccttc ttttttgtat ataaaaatcc caagtaaata cataaaaaat aaaaaaattg
       61 aaacttttag cgcaccttgt acgtcatatt aagtgaggca gaaaggagtg gagaatgatt
      121 tttactggat ataagaaaaa acggcagatc gaaaaatacc gtcacctgct gaatggactc
      181 atcgaaactg attatcaaaa aatgttccgt atcacgatga attacgtaca tgataaagaa
      241 gaggccttag atgtgatgca agatagtttt cataaagccc tgaactattt tgaaaaagaa
      301 aaagagatcg agcattttag tgcatggttt tatcggatat tgatccgcac ggcattagat
      361 agttggcgca gacaaaaaag gacgcctgtg gacttatttg aaattcaaga tatcccccaa
      421 cagttacctt tagaaacttc cgttgcagag ctccatatga ttttagataa aatagacagt
      481 ccggaaaaag agattctgat cctctacttt tttgaaggat ttcgtttgaa agaaattgcg
      541 actatcttgg atatgaatga aaacacagtt aaaacgaaaa tgtaccgctc attgaatgct
      601 ttacgtcaaa tcctagaatg aaggagagaa gagtgccatg tattcccaaa aagaaaattt
      661 aaaaaaacta aagaaaactt ataaagaaca agcaattccc aaagggctta aagaggagat
      721 ccgccagcgt tttattatcg aagagcaatc atttataaaa gcaaagcgga gaaagagacg
      781 tctccaaggg ttcggagtat ctttggctat cgtaatgatt acaacaagta gccttctttt
      841 taatagtcaa gttcgaagct ttgcagaaga tttacccatc ttaggatcag tcattgaatt
      901 gatcttaggc gaacgcttta ctgatcgatc tgaaaaaatc gatattcagg taccgaagat
      961 caatacacaa aatgagaaag aaaataaaac aattcatgga ttaaatcaaa aatactttcg
     1021 cgaaggacaa gcagattttg aaaaagctga aaaagaatat ggaaattttg aaacggatca
     1081 ttaccaagtt ttgggagatt atcagaaaat cgtagacgat aaccggtttc tcgtgattga
     1141 aaggcagatc acccaaacag cagcagatag ccatgttgaa aaaagatacg acacaatcga
     1201 caagaaaaac agtgtccagc tctcacttcc acttttattc aaagatgaca cctatctatc
     1261 tgttttgaca aaagaagtca aacggcagat ggccgaacaa gtcaaggaag atccatctaa
     1321 gtactattgg actgagcaag atattcaaga agggacgata gaaaaaccaa cacttgttac
     1381 gcctacacga agtttttatt tgaacaaaga gcatcaatta gttttgactt tttctcaata
     1441 cgaaattgct cctggttata tgggtacacc agaatttgtc attcctaagt ccgtgacaaa
     1501 aacgattttg gcatcggaag attatttgga tcattagacg agaagtagtt gcgactagca
     1561 gtttcaattt tcaagtaagt acaagtaaat aataagaaga gaaaaagaga aagaagagat
     1621 atagtgaaaa gaaattattt agttatcagt attattgctt tatccgtgct tttgtttttc
     1681 gaaattactt cggtggcctc cttgatcacc gaagtaaaag gacaaacaca ctcttcggag
     1741 cattcgtcac aatctttcaa acaatcagaa caatcaacca ttaaaagcca atcaactgaa
     1801 acagctaacg tcaaagaagc cattcaagaa tcagcggaaa aggtacaaag tcagtcatca
     1861 caatcaacac aagacagtac aaaaacgagc cgagatccgg ctgtcaaaag cgtcgcaatc
     1921 agctttgatg atggaccagg agcgaccaca acaccgcagc ttctgcgaat cttaaaagaa
     1981 aaaaacgtgc atgccacttt tttcgtctta ggagaaaata cggcgcagca tcctgaaatc
     2041 gtgaagcaga cagctgaagc agggcacgag attggcaatt acacatacga tcatcagaat
     2101 ctggccactc tttctgccca gtcgatgacg gaggaagtga cgaaagcaga cactgagatc
     2161 aaaaaagtca ctggcaaaac ccccactttt gttcgtccgc cctatggttc tgtcacgaat
     2221 gtaggagcaa cgatcatcca gcgaccgatc atcgaatggt ctgttgattc agaagattgg
     2281 aagacaagaa atcctgattt gattttgcag aagatccaag caacagtata cgatggggca
     2341 attattttat tccatgatat ctatcctgaa acgattcgtg ccgttcctca agtgatcgat
     2401 tatctgaaag agcaagggta tcgcatcacg acagttgggg atttattagg gcatccaacg
     2461 gctgtcgaga actattatgg cagaaacgat catcggccag ttcagtaagt gagacaagta
     2521 tctttaataa ataacacatt ttaataagta gttgaacaca taactacctt acttgcaaag
     2581 gcttaacaat atctatacag gtacaacaaa aaactgtcca ccaattttct ttggcggaca
     2641 gttttcactt aaaaataaag aagcaactat tcgtaaatta tgctaaaata gcagtagaac
     2701 gcgtcctgag ggaatcgaac ccccgtctca agaaccggaa tcttacgtga tatccactac
     2761 actaaggacg caagcactgg gataattgta actaaacaga taaaaaatta caaggggatt
     2821 ggacaaaaaa atgaaatttg agcaaaattt ttcacaaaaa cagcagcaga cacagaaact
     2881 agccatgacg caacagttgc aacagtccat ccaagtttta caatataaca gtgaggagct
     2941 gttggcattc gttgagaatc aagcaatgga aaatccattg gtggaagttg ttgagccgga
     3001 atggcagcca gattacataa aagcttcttc atcttcttat gaaggagaag agacaaatta
     3061 cctaaatcag attcctgata caaaagggtc tttatttgat tcattgatcg aacaagtcca
     3121 tttgaattac cgagatactt ttttgaggaa aatcgttctt tatcttgtag aatacatcga
     3181 tttgaacgga tttttgacga ttagtttaga agaagccgga aaagagattg gagctacgcc
     3241 tatccaaatg ctcgatgcgt tgactctgat tcagcaatta gaaccagctg gagtaggtgc
     3301 acgtagtttg caagaatgtc tcatgcttca aacagaacga gatgattatg cgccggaatt
     3361 agcttatatc gtgttggaag aatgttttga ggaactggtg gagcgaaaat ggaaagaaat
     3421 cgcacaacgt ttttctgttg atctacatgc tgttcagcag atattcgact atttacaaac
     3481 gttgagtcca agccctggcc ggattttcga ccgtagcagc gaattgtaca ttattcctga
     3541 tgtccgggta ttagtggacg acgataaaaa tgtacaagtt atctcaaacc gaaaaaatca
     3601 gccaaatata cgctttcaag agagttactt caaacaaatg tcacaacaag cagataaaga
     3661 aactgaaaat tatttaaaag aacggaaaca ggaattcgaa tggttgaaga aaacgatcct
     3721 acaacgtggg gatacaattt tgcgggttgc tcaagtcatt gtttcccggc aaaaagcttt
     3781 ctttatcgac aaggaacgtc caatcaagcc tcttacgtta aaggaagttg caactgagat
     3841 agatgtgcat gaatccacgg tcagtcgtgc agtcaacggg aaatatttgg aaacagattt
     3901 cggtgtattt gaattaaaaa aatttttcac tacacgaatt cctacgaata gtacagaaca
     3961 gacagaagac ctttcagcag atacagcgaa gaaaaagctg caggaattag tcgatcaaga
     4021 agacaaaaac aagccgttgt cggaccagaa attggttgaa ttgctgaaaa aagatgagat
     4081 agcgatctca agacgaacag tagcaaaata tcgtgattta ttaggaatcc cgagttcatc
     4141 aaaaagaaaa cgttatgata actaatcagt caaaagacta gaaaaagtat ttcacttttt
     4201 ctagtctttt tagttgaaaa acgagagcga aactgttaga cttacagtcg tgaggttgag
     4261 atttcccttt attgatgtga aattcgaata gttaatttct ttttttatcg tatatgggtc
     4321 gttttaagtc tacattggac gtaaagtgtc caactaagga gatcgctatg ctggatgaaa
     4381 taaaaatgat tgaatctgtt gcaccagata ttatcgaagt gttgcaagaa cgctataaaa
     4441 ttttacgaaa tatctattgg atgcagccga ttgggcgtcg aagtttatcg gaaagtatgg
     4501 ggatcacaga acgagtctta cgaacagaaa cagacctttt gaaaaatctg aatttgatcg
     4561 acacctcaaa aagcggtatg acgctgacaa ctaagggcga agaagtttat caaagccttg
     4621 aaaacttcat ggatcagctt ttaggtacgc atcaaaccga acaacagttg gcagaatatt
     4681 ttggtaacca acgctgtatc gttgtttcag gaaacagcga agagcagacc aaagtggctg
     4741 atgcattcgg cgaagcgctg agcgaagcct tagaccgact acttccagaa ggcgagaata
     4801 tcatcgctgt gatgggagga actactatgg ctgtggtagc cgaacaattg tctaatctgg
     4861 aaaataagaa acggcataac ctgtttgtcc ctgcaagagg cgggattggt gaagcgataa
     4921 ctgtacaggc aaattctgtc agtgcaagaa tggctgctaa agcaaacggt aaccaccgcg
     4981 cactttatgt tccggaacaa ttaagtctgg caacttacaa ttctttactc aatgagccat
     5041 ctatccaaga agtcttaaac ttgattagtg aagcgaattg tgttattcat agtattgggc
     5101 gtgcattaca catggcagca cgtcgtaaga tgactgaaaa agaattagtc atgctgaaac
     5161 aagcaaatgc tgttgcggaa tcttttggct actttttcaa tgaaaaaggt gaagtcgttt
     5221 ataaggtccc tagaatcggc atagaattgc aagatttgga aaaagtcccg atcatcatgg
     5281 caatcgcggg cggaaaatca aaagctaaag caattcgagc ttatatgaaa aatgcaccaa
     5341 aacaaacgtg gctcatcact gatgaagctg ctgcaaatga gattttaaaa ggggtaaccc
     5401 tttaaaataa atattttttt gattttcata aggaggaaat cttaaatgac agttaaagta
     5461 ggtattaatg gttttggacg tattggacgt ctagcattcc gtcgtatcca agatgtagat
     5521 ggaatcgaag tagtagcaat caacgacttg acagatgcta aaatgttggc acacttgtta
     5581 aaatacgaca caactcaagg acgtttcaac ggaacagttg aagttcatga aggttcattc
     5641 aacgtaaacg gaaaagaagt taaagtatta gctaaccgca acccagaaga attaccatgg
     5701 ggcgaactag gcgtagatat cgttctagaa tgtactggtt tcttcacttc taaatcagca
     5761 gctgaaaaac atttaacagc tggtgctaaa cgcgttgtta tctctgctcc tggcggaaac
     5821 gatgttccaa caatcgttta taacacaaac cacgaaacat taactggtaa agaaacagtt
     5881 atctcaggtg cttcatgtac tactaactgt ttagctccta tggctaaaac attgaacgac
     5941 aaatttggtg ttgttgaagg attaatgaca actatccacg cttacacagg tgaccaaatg
     6001 actctagacg gacctcatcc taaaggtgac ttccgccgtg cacgcgctgc tgctgcaaac
     6061 atcgttccta actcaacagg tgctgctaaa gctatcggtt tagtaattcc cgaattgaac
     6121 ggtaaattag acggagctgc tcaacgtgtt cctgtaccaa caggttcatt aacagaatta
     6181 gtaacagttc ttgaaaaaga agtaactgtt gacgaaatca atgcagcaat gaaagaagct
     6241 tcaaacgaat cttacggata caacacagac gaaatcgttt cttcagatat cgttggtatg
     6301 acttacggtt cattatttga tgctacacaa actaaagtaa tgacagttgg cgacaaacaa
     6361 ttagttaaaa ctgttgcttg gtacgacaac gaaatgtcat acactgcaca attagtacgt
     6421 actttagaat acttcgctaa cttataagat agacaaacgt acaagtgtaa attatagaca
     6481 tgtacattta aaagcgggga agcaatcgcg cttcttccgc tttttttgat aataagttta
     6541 ttcaggagga aataaaatgg ctaagaaaac agtgaaagat atcgatttaa aagacaaaaa
     6601 agttcttgtc cgtgttgact tcaacgttcc tttgaaagac ggcgtgatca ctgacgacac
     6661 tcgtatcaaa gcagcattgc caacaatcaa ctacgttcta gaacaaggcg gaaaagcaat
     6721 ccttttctct caccttggac gtgtaaaaac agaagaagat aaagaaggaa aatctttagc
     6781 tccagttgca aaacgtctag gcgaattatt aggtaaagaa gtaacattcg tacctgaaac
     6841 tcgcggagaa caattagaag aagcaatccg caacatgaat gacggcgacg ttgtcgtatt
     6901 cgaaaataca cgatttgaag atattgacgg taaaaaagaa agcaaaaatg atacagaact
     6961 aggtaaatac tgggcttcat taggcgatgt atttgttaac gatgctttcg gtacagctca
     7021 ccgtgcacat gcttctaacg taggtatcgc ttcaactggt atcccaacag ttgctggatt
     7081 cttgatggaa aaagaaatca agttcatcgg tgaagcagta gaagaaccaa aacgaccaat
     7141 gattgcaatc cttggtggcg caaaagtttc tgataaaatc ggcgtaatcg aaaacttgat
     7201 tcctaaagca gataaaatct tgatcggtgg cggaatgact tacacattct acgaagcaaa
     7261 aggaatcaag atcggtaact ctctagtaga agctgataaa gtggaactag caaaagaatt
     7321 aatcgaaaaa gcaggcgaca aacttgtctt gccaatcgac aatgtctgtg caccagaatt
     7381 ttcaaatgat gttgaaacac aagtaatcga aggagacatt cctgatggtt tgatggcttt
     7441 agacatcggc ccagcatcag taaaattatt tgctgataca ttacaaggtg caaaaacagt
     7501 tgtttggaac ggacctatgg gtgtatttga aatgagtaac tttgctaaag gtacaatcgg
     7561 tgtttgtgaa gcaatcgcta acttagaagg cgcaacaaca atcatcggtg gcggagactc
     7621 agccgcagca gcagaacaat taggatttgc tgacaaattc acacacatct caacaggtgg
     7681 cggcgcaagc ttggaattgc ttgaaggtaa agaattacca ggactagcag caatcaacga
     7741 taaataaacg accaagtttg ctcaattctg acgagtaatc gaacaaaagg tgaagggagt
     7801 tttcatgact ccctagccat ttttcaataa attaaggagt gtgttcctca atgcgtaaac
     7861 ctattatcgc aggtaactgg aaaatgaaca aaactgcatc agaagcaaaa gaatttgctg
     7921 aagcagtaaa aaacaaaatc ccttcaaatg acaaagttga ttcagtcatc ggatctcctg
     7981 cattattctt acaagaatta atgacatcag ctgaaggtac agaactaaaa atctcagcac
     8041 aaaactgcta ctgggaaaac tcaggtgcgt tcactggtga aacttcacca gcagctcttg
     8101 ctgatctagg tgttcattat gtgatcatcg gacattcaga acgtcgtgaa tatttccatg
     8161 aaacagacga agacatcaat aaaaaagcga aagctatctt tgcaaatggt atgacaccaa
     8221 tcctatgctg cggcgaatct ttagaaacct atgaagcagg caaaactgct gaatggatcg
     8281 aaggacaaat tacagctggt ttagctgatc tttcagctga acaggtgtca aacatggtta
     8341 ttgcttatga accaatctgg gcaatcggta ctggtaaatc agctgacgca caaatcgctg
     8401 acgaaatttg tggagttgta cgtcaaacag ttgaaaaact ttatggaaaa gaagtatcag
     8461 aagcagttcg tatccaatac ggcggttctg taaaaccaga aaatattgct gaatacatgg
     8521 caaaagaaaa tgttgacggt gctttagtcg gtggcgcaag cttgcaagca gattcattct
     8581 tgtcattgtt agacgctgta aaataattgt tgaaaatttg cgaaaaatgc aagaaatgtt
     8641 tgcactaatg agcaaaatta actaaaatcg tatatgaggg aaatccctta ggaaaaaaca
     8701 aactcaaagg agagacaaaa catgtcaatt attactgatg tttacgctcg cgagatctta
     8761 gactcacgtg gtaacccaac aatcgaagta gaagtataca cagaaagcgg agcttttggc
     8821 cgcggaatgg ttccttcagg tgcttcaact ggtgaatacg aagcagttga attacgtgac
     8881 ggagacaaag ctcgttatgg cggtaaaggt gtaactaaag ctgtcgacaa cgtaaacaac
     8941 atcattgctg aagcaatcat tggctacgat gttcgtgacc aaatggctat cgacaaagct
     9001 atgatcgctt tagacggaac tcctaacaaa ggtaaattag gtgcgaacgc tattttaggt
     9061 gtttctatcg ctgttgcacg tgcagctgct gactacctag aagtaccttt ataccactac
     9121 ctaggcggat tcaacacaaa agtattgcca acaccaatga tgaacatcat caacggcgga
     9181 tcacatgctg ataactcaat cgacttccaa gaattcatga tcatgcctgt tggcgctcca
     9241 acattcaaag aagctctacg tatgggtgct gaagtattcc acgcattagc ttcaatcttg
     9301 aaagctcgcg gattagcaac ttctgttggt gacgaaggtg gatttgctcc aaaccttggt
     9361 tcaaacgaag aaggttttga agtaatcatc gaagcaatcg aaaaagctgg ctatgtacct
     9421 ggtaaagacg ttgttcttgc tatggatgct gcttcttcag aattctacga caaagaaaaa
     9481 ggtgtttacg tactagctga ctcaggcgaa ggcgaaaaaa caactgacga aatgatcaaa
     9541 ttctacgaag aattagtttc taaatatcca atcatctcaa tcgaagatgg attagacgaa
     9601 aacgactggg acggattcaa gaaattaact gacgtattag gcgacaaagt tcaattagtt
     9661 ggtgacgact tgttcgtaac aaacactcaa aaattatctg aaggtatcga aaaaggaatt
     9721 gctaactcaa tccttatcaa agtgaaccaa atcggtacat taacagaaac tttcgaagct
     9781 atcgaaatgg ctaaagaagc tggctacaca gcagttgtat ctcaccgttc tggtgaaaca
     9841 gaagattcaa caatctctga tatcgctgtt gcaacaaacg ctggccaaat caaaactggt
     9901 tctctatcac gtactgaccg tattgctaaa tacaaccaat tactacgtat cgaagaccaa
     9961 cttggtgaag ttgcagaata caaaggtttg aaatctttct acaacttaaa aaacaaataa
    10021 ttcttttcta atgaagatac atgatcgact attgaaagat agtcgagtat gatcacttag
    10081 ttaagtaatc gaactctcta cttgctgaaa tatcagcagg tagagagttt ttttgcaact
    10141 agcttctgta tatgatgttt acaaagaatg gtacaagaaa agatcatcct tgaaaaagga
    10201 aagctaacaa taactagcat catataagag cttttaagga tagaacagta gaataggaat
    10261 ctatgggcaa gtcctctcgt ataaataaaa taggagggaa gtagcagcat atttttcata
    10321 aagtggctct ctttcttcat ttttactttg gttgctccta tttgaaaatt cgagcacact
    10381 caaatacaat aaaaagaaat ggcaagggag aggattagat gaaaaaagaa gaaaaaatcg
    10441 cgattttaca agagattatc cgaattaaat cagtgaatgg aaacgaaggt gaagtagcag
    10501 cctatttgaa taagcttttg gcaaagcatg acataacagg agagattgtt tcttacagag
    10561 atggtagaga taatttgatt gctcgttacc aaaagggcca atcaggaaaa gtgttaggcc
    10621 tatctggaca tatggatgtc gtggcggctg gtgatgaatc gtcttggacc tatgcaccct
    10681 ttgcagcaga aatccatggg aaccgtttgt acggacgtgg tgctacagat atgaagagtg
    10741 gcttggcagc aatggtgatt gccatgatcg aactgaaaga gtcaggaaaa ccatttaacg
    10801 gaacagtcaa attattagcc actgtcggag aagaagtggg tgaactaggt ggggaacagc
    10861 tcacgaaagc aggctatgta gatgacttag atgccttgat catcggagag ccaaccaatt
    10921 acagtttgat gtatacgcac atgggatcaa tcaactatac agtcacctcg cacggaaaag
    10981 aagcgcatag ctctatgcca gaccaaggtt ataatgcaat caatcaccta aatgaattca
    11041 ttacgaaagc aaatgctgaa atgaatcacc tcgctgaaac aatcgagaat cctgtcctag
    11101 gaaaaacaat ccataatgtt acattgatca gtggcgggaa tcaagtcaac agcatcccaa
    11161 gccatgcgca gctccaaggg aacattcgat cgattccaga atacccaaat gataaaatca
    11221 ttgcattgct gcaatcaatc gtcaatgaat tgaatcaaga gacagactat catttggaat
    11281 tgatgatcga ttacaacaaa atacctgtca aagctgatcc agattctcct ttgattcatt
    11341 gcattcagca acaattcagc cagccattac ctttagttgg agcggcagca acaacggatg
    11401 cagccgaatt tacgaaagct aaccattcct tcgactttgt cgtctttggg cctggtgtcg
    11461 ttactttgcc tcatcaagta gatgagtatg ttgaaatcga taattatttg gatatgatcg
    11521 aaaaatatca aggaattatt ttatcctact tagcgtaaga tttttttaat aa
//
LOCUS       DASHFF010000079        11382 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.079, whole genome shotgun sequence.
ACCESSION   DASHFF010000079 DASHFF010000000
VERSION     DASHFF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.079"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(313..903)
                     /locus_tag="U2I75_002381"
     CDS             complement(313..903)
                     /locus_tag="U2I75_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN1697467.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1426..1587
                     /locus_tag="U2I75_002382"
     CDS             1426..1587
                     /locus_tag="U2I75_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1697468.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1606..2100
                     /locus_tag="U2I75_002383"
     CDS             1606..2100
                     /locus_tag="U2I75_002383"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1697469.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            2093..2254
                     /locus_tag="U2I75_002384"
     CDS             2093..2254
                     /locus_tag="U2I75_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697470.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2738..3761
                     /locus_tag="U2I75_002385"
                     /pseudo
     CDS             2738..3761
                     /locus_tag="U2I75_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4104..4360
                     /locus_tag="U2I75_002386"
                     /pseudo
     CDS             4104..4360
                     /locus_tag="U2I75_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            4747..4974
                     /locus_tag="U2I75_002387"
     CDS             4747..4974
                     /locus_tag="U2I75_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN1697471.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4990..6156
                     /locus_tag="U2I75_002388"
     CDS             4990..6156
                     /locus_tag="U2I75_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEN1697472.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6391..7434
                     /gene="hrcA"
                     /locus_tag="U2I75_002389"
     CDS             6391..7434
                     /gene="hrcA"
                     /locus_tag="U2I75_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN1697473.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7528..8091
                     /gene="grpE"
                     /locus_tag="U2I75_002390"
     CDS             7528..8091
                     /gene="grpE"
                     /locus_tag="U2I75_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN1697474.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            8142..9971
                     /gene="dnaK"
                     /locus_tag="U2I75_002391"
     CDS             8142..9971
                     /gene="dnaK"
                     /locus_tag="U2I75_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN1697475.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            10122..11288
                     /gene="dnaJ"
                     /locus_tag="U2I75_002392"
     CDS             10122..11288
                     /gene="dnaJ"
                     /locus_tag="U2I75_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN1697476.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         4002 a         1830 c         2329 g         3221 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagttttt tattttaatg aattaagact attaaataga taattattgt aacttaaaaa
     2341 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     2401 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     2461 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     2521 tgtttttaca taagaagact tcgggtattt tttagtttga ttagctctga tatacctata
     2581 aagaatatgc taaggtaaga atagtaaaaa ttcacataga tgaggtataa taatgatatt
     2641 tttatatatt acagataact tgacacaaaa tgagtttttt tcgatttttc aagttatcta
     2701 atgcaattat attgatatca ttaggagctg cattgttatg aatccaacat tgattactaa
     2761 gaaagaatta ctaaaaaaat tagatatttc tactggtgta ttagctaatt taattagaaa
     2821 tggcatgcca aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa
     2881 ttggtctaaa aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat
     2941 ttcagagaag tttaaatgtt ccaaacaggg tggaatgaga agatctcatc aaactaatac
     3001 tcttgtactg tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg
     3061 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
     3121 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
     3181 accaaaagaa cacacatatc tgggagaagt atatttagca ggacaaatat atactgttaa
     3241 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
     3301 tcaagagcaa ttgattgaag ataaagatat ataaccagga agaaaatcaa actagacata
     3361 ttcgtaattt atctaatgct aaattagagg aagaagcaag aaaagttagt aattataata
     3421 aaaaaatggc tgcaaaatat gttaacgaag aggagttttg cttctcaaaa acaaaaatat
     3481 ataaacgtaa tcccttcgta tctgaatatg ttaaacgact tgcaaaaggt atttgtcaac
     3541 tttgccaaga aaaagggcca tttataaaag acggtgtacc ttatttacat tgtcatcata
     3601 tagaatattt gtcgcaaggt ggaaaagatg tgattgaaaa ctgtatcgcg ttatgtccca
     3661 attgtcatgc tagaattcat gaacttgagt tacctgacga taaagaaaaa ttgctgaaga
     3721 tcgttgaaaa aagagaaaat agcttttcta aaataccgta atttattttt ataggacaaa
     3781 taagctaatg tgagctatgc cttctatata agactaagtt ctattataag taacgaagta
     3841 attctcgttc cttcctgtag taatattttt taaataacct ataggcttct tcaaaagtac
     3901 atcctccagt aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa
     3961 aaagtagttg gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg
     4021 atcatttact acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt
     4081 tctaaaatca catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga
     4141 ttacggttcg tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg
     4201 ggattacttt atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca
     4261 atcgtcaaac ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat
     4321 tcatggaaga atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg
     4381 cccgaaattc agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa
     4441 tcaattcgtt gttatcgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac
     4501 tgtttatttt tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat
     4561 ttgactagat ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg
     4621 taaaaaaatc atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac
     4681 gtttagtatg cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag
     4741 gaatctgtga aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc
     4801 tttgcgatta ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag
     4861 caagcatttt tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg
     4921 gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa
     4981 agaatttcaa tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat
     5041 atcccatttt gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg
     5101 cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt
     5161 catcctacag cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct
     5221 gctaaacaac ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt
     5281 aatgagttta ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg
     5341 aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg
     5401 aaaaaaattg gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa
     5461 gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta
     5521 gaaggatatc gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg
     5581 tactcattga tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc
     5641 gaactgcctg atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa
     5701 aagcatggac gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa
     5761 cataacctca tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt
     5821 tatcttggag atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta
     5881 cgagcaaatg aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa
     5941 gaagaaatgt tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat
     6001 cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa
     6061 gggttacttg ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg
     6121 aacaatgtgt ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc
     6181 gaaacttgaa aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa
     6241 ctggatttac atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga
     6301 gtctttctct tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga
     6361 atatatgagt gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta
     6421 cgtctgatca tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg
     6481 tctgagggag tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat
     6541 gaaggattgc tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat
     6601 cgttattacg tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg
     6661 attcgtcagt ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct
     6721 gagatattat ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac
     6781 cgccgactga ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc
     6841 gtaacagata tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt
     6901 caggaactag aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg
     6961 acggtgtacc ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg
     7021 acagaaggga tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt
     7081 tatgtcagtg gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc
     7141 aaatcgatgt tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg
     7201 gatagcaata ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg
     7261 agtatgattc aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta
     7321 gggcctacta gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa
     7381 ctagcagcta aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa
     7441 taacgaagct atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg
     7501 actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa
     7561 atgacagatg ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt
     7621 tcagaagctg aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg
     7681 gaagataaat acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat
     7741 gagcgtgagc aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca
     7801 attgacaacc tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg
     7861 aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt
     7921 gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact
     7981 gttccagcga ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac
     8041 aaattacatg atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga
     8101 tataaaactt acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta
     8161 ttgacttagg tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca
     8221 tcgctaaccc agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa
     8281 tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa
     8341 tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac
     8401 cacaagaaat ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag
     8461 gtgaaaaagt cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc
     8521 aagcaacaaa agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac
     8581 caactgcagc tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag
     8641 tattcgacct tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct
     8701 ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa
     8761 tcatcgacta catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca
     8821 aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg
     8881 tatcaagcac acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt
     8941 tggaaatgac attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta
     9001 caaaagtacc agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg
     9061 aagttatttt agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag
     9121 aaacaggaaa agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg
     9181 ctatccaagg tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac
     9241 cattgtcatt aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata
     9301 caacgattcc aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag
     9361 tagacatcca tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa
     9421 gattccaatt gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat
     9481 tcgatattga taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag
     9541 aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg
     9601 tgaaagatgc agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac
     9661 gtaatgatgt agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca
     9721 aagtggacga agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg
     9781 ttgaagcaaa caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc
     9841 aaaacttgac tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag
     9901 aagcagctca aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg
     9961 acgataaata atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt
    10021 tcttttgaaa aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac
    10081 aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt
    10141 attatgaagt cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc
    10201 gtaagctttc gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat
    10261 tcaaagaagt atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg
    10321 accaatacgg gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat
    10381 ttggtggatt cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact
    10441 ctttctttgg aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt
    10501 tgcaatatac ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca
    10561 agtataatcg ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac
    10621 aaccaactac ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc
    10681 cacttggacg agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa
    10741 tcaaagaacc ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta
    10801 aagtgaatgt accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag
    10861 aagctggaga aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa
    10921 gtgatatctt tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac
    10981 aagcagcttt gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga
    11041 tccctgctgg aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt
    11101 tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc
    11161 taaatgaaca acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg
    11221 aagaacagca agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc
    11281 ggaagtagtt tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta
    11341 aaaacgctaa gtcattaata attaaaaacc agacgaacaa tc
//
LOCUS       DASHFF010000080        11265 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.080, whole genome shotgun sequence.
ACCESSION   DASHFF010000080 DASHFF010000000
VERSION     DASHFF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.080"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            6..305
                     /locus_tag="U2I75_002393"
     CDS             6..305
                     /locus_tag="U2I75_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1697477.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U2I75_002394"
     CDS             378..1739
                     /locus_tag="U2I75_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN1697478.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U2I75_002395"
     CDS             1761..2021
                     /locus_tag="U2I75_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697479.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U2I75_002396"
     CDS             2035..2787
                     /locus_tag="U2I75_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN1697480.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U2I75_002397"
     CDS             complement(2881..3486)
                     /locus_tag="U2I75_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1697481.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="U2I75_002398"
     CDS             complement(3542..4516)
                     /locus_tag="U2I75_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN1697482.1"
                     /translation="MYTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="U2I75_002399"
     CDS             complement(4894..5613)
                     /locus_tag="U2I75_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1697483.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="U2I75_002400"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="U2I75_002400"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1697484.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="U2I75_002401"
     CDS             7164..8561
                     /locus_tag="U2I75_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1697485.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="U2I75_002402"
     CDS             8642..9082
                     /locus_tag="U2I75_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1697486.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="U2I75_002403"
     CDS             9229..9555
                     /locus_tag="U2I75_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1697487.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="U2I75_002404"
     CDS             9556..10305
                     /locus_tag="U2I75_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697488.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="U2I75_002405"
     CDS             complement(10331..10741)
                     /locus_tag="U2I75_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1697489.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3590 a         1964 c         2309 g         3402 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atacatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa tcgcgaattg
    11161 taaaattaag ttagaaaaat aaaaaggcat ttatggtaca ctcaaattgt atttccgacg
    11221 aaagaaaaca aaggagtgta accataaatg acctacaaac atctt
//
LOCUS       DASHFF010000081        11254 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.081, whole genome shotgun sequence.
ACCESSION   DASHFF010000081 DASHFF010000000
VERSION     DASHFF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.081"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="U2I75_002406"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="U2I75_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN1697490.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="U2I75_002407"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="U2I75_002407"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN1697491.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="U2I75_002408"
     CDS             complement(2169..2906)
                     /locus_tag="U2I75_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1697492.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="U2I75_002409"
     CDS             complement(2997..3524)
                     /locus_tag="U2I75_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697493.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="U2I75_002410"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="U2I75_002410"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN1697494.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="U2I75_002411"
     CDS             complement(4401..4886)
                     /locus_tag="U2I75_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN1697495.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="U2I75_002412"
     CDS             complement(5039..5236)
                     /locus_tag="U2I75_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697496.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="U2I75_002413"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="U2I75_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN1697497.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="U2I75_002414"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="U2I75_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN1697498.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="U2I75_002415"
     CDS             9211..9423
                     /locus_tag="U2I75_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN1697499.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="U2I75_002416"
     CDS             9425..11104
                     /locus_tag="U2I75_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1697500.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DASHFF010000082        10928 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.082, whole genome shotgun sequence.
ACCESSION   DASHFF010000082 DASHFF010000000
VERSION     DASHFF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.082"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..154
                     /locus_tag="U2I75_002417"
     CDS             <1..154
                     /locus_tag="U2I75_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN1697501.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /gene="istA"
                     /locus_tag="U2I75_002418"
                     /pseudo
     CDS             complement(235..1218)
                     /gene="istA"
                     /locus_tag="U2I75_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="U2I75_002419"
     CDS             complement(1613..2455)
                     /locus_tag="U2I75_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1697502.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="U2I75_002420"
     CDS             2780..3196
                     /locus_tag="U2I75_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1697503.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="U2I75_002421"
     CDS             3193..3663
                     /locus_tag="U2I75_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1697504.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="U2I75_002422"
     CDS             3677..4450
                     /locus_tag="U2I75_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1697505.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="U2I75_002423"
     CDS             4464..5276
                     /locus_tag="U2I75_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1697506.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="U2I75_002424"
     CDS             5330..6295
                     /locus_tag="U2I75_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1697507.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="U2I75_002425"
     CDS             6432..9155
                     /locus_tag="U2I75_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1697508.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="U2I75_002426"
     CDS             9168..9800
                     /locus_tag="U2I75_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1697509.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10005..10232
                     /locus_tag="U2I75_002427"
     CDS             10005..10232
                     /locus_tag="U2I75_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEN1697510.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10301..10567
                     /locus_tag="U2I75_002428"
     CDS             10301..10567
                     /locus_tag="U2I75_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1697511.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="U2I75_002429"
     CDS             10557..10826
                     /locus_tag="U2I75_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1697512.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3625 a         1741 c         2136 g         3426 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaagagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttct
//
LOCUS       DASHFF010000083        10542 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.083, whole genome shotgun sequence.
ACCESSION   DASHFF010000083 DASHFF010000000
VERSION     DASHFF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.083"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            23..646
                     /locus_tag="U2I75_002430"
     CDS             23..646
                     /locus_tag="U2I75_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697513.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="U2I75_002431"
     CDS             1096..2145
                     /locus_tag="U2I75_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1697514.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="U2I75_002432"
     CDS             2142..3023
                     /locus_tag="U2I75_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1697515.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="U2I75_002433"
     CDS             3026..3796
                     /locus_tag="U2I75_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1697516.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="U2I75_002434"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="U2I75_002434"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN1697517.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGITIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="U2I75_002435"
     CDS             5107..6270
                     /locus_tag="U2I75_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1697518.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="U2I75_002436"
     CDS             6387..6797
                     /locus_tag="U2I75_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1697519.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="U2I75_002437"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="U2I75_002437"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1697520.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="U2I75_002438"
     CDS             9370..9654
                     /locus_tag="U2I75_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697521.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="U2I75_002439"
     CDS             9814..10050
                     /locus_tag="U2I75_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1697522.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="U2I75_002440"
     CDS             10047..10445
                     /locus_tag="U2I75_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN1697523.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3673 a         1770 c         2230 g         2869 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat tacgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aa
//
LOCUS       DASHFF010000084        10496 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.084, whole genome shotgun sequence.
ACCESSION   DASHFF010000084 DASHFF010000000
VERSION     DASHFF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.084"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..360)
                     /locus_tag="U2I75_002441"
     CDS             complement(<1..360)
                     /locus_tag="U2I75_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN1697524.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGI"
     gene            complement(712..1071)
                     /gene="rplT"
                     /locus_tag="U2I75_002442"
     CDS             complement(712..1071)
                     /gene="rplT"
                     /locus_tag="U2I75_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN1697525.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="U2I75_002443"
     CDS             complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="U2I75_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN1697526.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1396..1920)
                     /gene="infC"
                     /locus_tag="U2I75_002444"
     CDS             complement(1396..1920)
                     /gene="infC"
                     /locus_tag="U2I75_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN1697527.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1964..2089)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2240..2872
                     /locus_tag="U2I75_002445"
     CDS             2240..2872
                     /locus_tag="U2I75_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1697528.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2874..3779)
                     /locus_tag="U2I75_002446"
     CDS             complement(2874..3779)
                     /locus_tag="U2I75_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1697529.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3864..4523)
                     /locus_tag="U2I75_002447"
     CDS             complement(3864..4523)
                     /locus_tag="U2I75_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1697530.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4839..5783
                     /gene="mvk"
                     /locus_tag="U2I75_002448"
     CDS             4839..5783
                     /gene="mvk"
                     /locus_tag="U2I75_002448"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN1697531.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5776..6753
                     /gene="mvaD"
                     /locus_tag="U2I75_002449"
     CDS             5776..6753
                     /gene="mvaD"
                     /locus_tag="U2I75_002449"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN1697532.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6766..7851
                     /locus_tag="U2I75_002450"
     CDS             6766..7851
                     /locus_tag="U2I75_002450"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN1697533.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7848..8912
                     /locus_tag="U2I75_002451"
     CDS             7848..8912
                     /locus_tag="U2I75_002451"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN1697534.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9161..9964)
                     /locus_tag="U2I75_002452"
     CDS             complement(9161..9964)
                     /locus_tag="U2I75_002452"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN1697535.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="U2I75_002453"
                     /pseudo
     CDS             complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="U2I75_002453"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10271..>10496)
                     /locus_tag="U2I75_002454"
     CDS             complement(10271..>10496)
                     /locus_tag="U2I75_002454"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEN1697536.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3106 a         2220 c         2008 g         3162 t
ORIGIN      
        1 aatacctaat gacatcaaga tcggcaacac aataactgca atagaaatca ctggtccgat
       61 tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct
      121 tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc
      181 tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta
      241 ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat
      301 cgccgagttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat
      361 gatgacaaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga
      421 atgagataac taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt
      481 gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg
      541 ctttcattaa aggatgacca taaatgttca gttttcaatt aaaaaacctt aattatgtta
      601 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
      661 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      721 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      781 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      841 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      901 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      961 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
     1021 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
     1081 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
     1141 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
     1201 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
     1261 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
     1321 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
     1381 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
     1441 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
     1501 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
     1561 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1621 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1681 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1741 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1801 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1861 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1921 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1981 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     2041 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatta
     2101 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     2161 gaaaatgatt agattagcag gctttctagc agtgattccc gcttctttgc tatactaaag
     2221 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     2281 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     2341 aggtaaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     2401 acttacgatg caaaaaacag gtttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     2461 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     2521 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     2581 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2641 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2701 caatcagtta accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2761 taatttcttc acaacaatcg ctttatctca agattctttg gcgggactaa tcgtcaatca
     2821 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2881 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2941 atttcccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     3001 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     3061 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     3121 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gtgcatcgtc caactcttct
     3181 ttttcctttt ctgacaactt gcgataaata gccagtgcct tgatttgttc taaaagtact
     3241 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     3301 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     3361 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     3421 aaactgtata cactcatgtc tggcatccgt tccaaaagcg atcgaattag tcgaatcacg
     3481 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     3541 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3601 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3661 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3721 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3781 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3841 ccctttattt gagcaactgt aaatcaaatg gatactttgg tttggcgaat ctctgctaat
     3901 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3961 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcataa
     4021 cgtctgttca attctttgta ataatcgtat agacttgggt caaaggacaa ttgttcatac
     4081 gaacgaccac gtttttcgat acgctctaac atcgtatcaa aagatacacg aatatgaact
     4141 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     4201 agttcatcat acacacaaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     4261 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     4321 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     4381 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     4441 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     4501 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     4561 aactccaacc ttttttaaat atacccccaa tatctagtag tgtcaattgt attttttcta
     4621 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4681 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4741 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4801 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4861 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4921 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4981 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     5041 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     5101 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     5161 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     5221 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     5281 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct tttgactact
     5341 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     5401 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     5461 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gagaattcac
     5521 cagaaacgtt ggcacagaca atggatgaat cacagtcact tctggaaaag ctgacaatca
     5581 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5641 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5701 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5761 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5821 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5881 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5941 gaattttatc tagatggcac cttacaagac gaaaaagcaa caaaaaaagt cagtcagttt
     6001 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     6061 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcagga
     6121 gcttgcaata ctgctcttaa gcttggatta gacgatctct ctctttcaag atttgctcga
     6181 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgttgaatg ggaaaaaggc
     6241 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     6301 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     6361 cgtcgaacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     6421 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     6481 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     6541 tggtctcctg acagcttaaa agcgatggat gcggtgcggc aattgagaaa aaaaggcatt
     6601 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6661 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgcc
     6721 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6781 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg acatccagca
     6841 gttatcgctg cagtcgatca attcgtaaca gtcactgtag aatccgcacg aaaagtcgga
     6901 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6961 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgtttgact
     7021 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     7081 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     7141 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcaatt ggagattttc
     7201 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     7261 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     7321 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     7381 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     7441 tctacttctg atttggtcga tcaagttcac cgttcgagag aagacaaaat ggtggcttat
     7501 cagcttttct taaaaaacag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     7561 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7621 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7681 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg gtggcgattg cggaatcgta
     7741 attgttgacc agaaatctgg catccttcct ttaatgagtg catgggaaaa agcagaaatc
     7801 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7861 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7921 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7981 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     8041 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     8101 ttgccactgg ttcagtcagc gctgccttga aagacccttc tttagccgat acttatacga
     8161 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     8221 tcgaaagagc ccaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     8281 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     8341 ttattcaaga gactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     8401 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     8461 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     8521 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     8581 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8641 aagccctttg tctcggcgca aatgcagtgg gtacttcagg aaccgtactg acgcatctga
     8701 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8761 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tcactgatct
     8821 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8881 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttatcccac gctctaaaca
     8941 cgtataaacg gcggaagcaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     9001 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaacgctt ctgtcccagc
     9061 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caagccgctg
     9121 atttcacaac ctctccacta aacggtgtac taaagctctt ttaaggttgt tttccgatat
     9181 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     9241 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     9301 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     9361 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     9421 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     9481 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     9541 taccgccgcc tttttcgatc atatcccgaa taactgcttt tgaaacgatg aatggggcat
     9601 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9661 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9721 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9781 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9841 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9901 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9961 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
    10021 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
    10081 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac gactaaatgt
    10141 tttgtttcat ctggtttgcc catcatatgg aacacacgtg tatcttcatt ggcataatca
    10201 aaatagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagca
    10261 cttcctggtt ctaaaatcgt ataacccatt tataaatgac agctatcgct gaagcttcta
    10321 ctgctgtaaa tatccccgtc aagataccac cgataatgat gacaatcaat agaaggctag
    10381 gaatcgcctc ttttattatt tttccagcat gagccatctg tcctttttcg acaacagggt
    10441 atttattctt tttagcaacg atgaatgcta ctagcatgat tcctagcgcc cacaaa
//
LOCUS       DASHFF010000085        10243 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.085, whole genome shotgun sequence.
ACCESSION   DASHFF010000085 DASHFF010000000
VERSION     DASHFF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.085"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            160..390
                     /locus_tag="U2I75_002455"
     CDS             160..390
                     /locus_tag="U2I75_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN1697537.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            487..1788
                     /gene="murA"
                     /locus_tag="U2I75_002456"
     CDS             487..1788
                     /gene="murA"
                     /locus_tag="U2I75_002456"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1697538.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1801..1977
                     /locus_tag="U2I75_002457"
     CDS             1801..1977
                     /locus_tag="U2I75_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1697539.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2112..2408
                     /gene="yidD"
                     /locus_tag="U2I75_002458"
     CDS             2112..2408
                     /gene="yidD"
                     /locus_tag="U2I75_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN1697540.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2592..3059
                     /locus_tag="U2I75_002459"
     CDS             2592..3059
                     /locus_tag="U2I75_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1697541.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3144..4028
                     /locus_tag="U2I75_002460"
     CDS             3144..4028
                     /locus_tag="U2I75_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1697542.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4142..4378
                     /locus_tag="U2I75_002461"
     CDS             4142..4378
                     /locus_tag="U2I75_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN1697543.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4466..4546
                     /locus_tag="U2I75_002462"
     tRNA            4466..4546
                     /locus_tag="U2I75_002462"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4500..4502,aa:Tyr,seq:gta)
     gene            4553..4624
                     /locus_tag="U2I75_002463"
     tRNA            4553..4624
                     /locus_tag="U2I75_002463"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4585..4587,aa:Gln,seq:ttg)
     gene            4749..4919
                     /locus_tag="U2I75_002464"
     CDS             4749..4919
                     /locus_tag="U2I75_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697544.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5139..5846
                     /locus_tag="U2I75_002465"
     CDS             5139..5846
                     /locus_tag="U2I75_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1697545.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5906..6550)
                     /locus_tag="U2I75_002466"
     CDS             complement(5906..6550)
                     /locus_tag="U2I75_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN1697546.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6677..6964
                     /locus_tag="U2I75_002467"
     CDS             6677..6964
                     /locus_tag="U2I75_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697547.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7072..7392
                     /locus_tag="U2I75_002468"
     CDS             7072..7392
                     /locus_tag="U2I75_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1697548.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7473..8786
                     /locus_tag="U2I75_002469"
     CDS             7473..8786
                     /locus_tag="U2I75_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1697549.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8783..9502
                     /locus_tag="U2I75_002470"
     CDS             8783..9502
                     /locus_tag="U2I75_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN1697550.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9630..10181
                     /gene="raiA"
                     /locus_tag="U2I75_002471"
     CDS             9630..10181
                     /gene="raiA"
                     /locus_tag="U2I75_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN1697551.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3415 a         1693 c         2187 g         2948 t
ORIGIN      
        1 ttgtctcagt cccttttttt gcatattttt agcatttatt tcctaaaaaa tgtaagggga
       61 ttcctatatt tttaagtata cttgtttttc cttttataca ttttatggta ttatcatttt
      121 attagttatt ataaaacaat cgttagaaag gacgtcatta tgcaagtata cggaattgat
      181 gcggtgcttc gaatcgtgag tcatttggcg tttatctact tagccttctg gtctttacgt
      241 tcgttacgca ttgaaacttt tttcagacca atgcataata cgcaaattag aatggcaatc
      301 gtactttttt caattttttt agggtacact gcaagtaatt ttttcttaga agttatcgct
      361 ttatgccgaa atcttttcgt tagcctgtaa attttaaaaa aaggtactat tttaaaagca
      421 aggatgttat aatagaaaag attttgagta agtgtatgct cattggaatt ttttggaggg
      481 aacaacatgg aagagatcat cgtaagaggt ggcaatcaat taaatggaac agtaagaatt
      541 gaaggagcta aaaatgctgt tttgcctatt ttagctgcta gtttgttagc agaagaaggt
      601 attacgacat tagacaacgt tccgattctt tctgatgtct tcactatgaa tcaagtgatt
      661 cgtcacttaa acgtagatgt agattttgat gaacaaaaga atcaagtaac aatcgatgct
      721 tcaagacaat tagaaatcga agcgccttat gagtatgtta gtcaaatgcg tgcttcaatc
      781 gttgtgatgg gtccgctact tgccagaaat ggacatgcaa aagtagctat gcctggtgga
      841 tgtgcgattg gtaaacgacc aatcgatttg catttaaaag gatttcaagc tttaggagca
      901 aaaattattc aaaaaaacgg ctatatcgaa gcaattgcgg atgaattgat cggtaacacg
      961 atttatttag atttccctag tgtaggtgct actcaaaata tcatgatggc tgccgtaaaa
     1021 gcaaaaggaa caaccatcat tgaaaacgta gctcgcgaac cagaaatcgt tgatttagca
     1081 aacattttga ataaaatggg cgctcaagtc tatggagctg gtactgaaac aatgcgtatt
     1141 gaaggcgtgg atcatttaca tgcagtcaac cactcaatcg tacaagatcg tatcgaagca
     1201 ggtaccttta tggttgctgc tgcaatgaca caaggcaatg tgctgattgc agatgctatc
     1261 tctgaacata accgcccatt gatttctaaa ttgatcgaaa tgggtgcaga aatcattgaa
     1321 gaagaaggcg gagtccgtgt catcggacca aaacatatct tacctacaga tgtaaaaacg
     1381 atgcctcatc ctggtttccc tacagatatg caagctcaaa tgacagctat ccaattagtt
     1441 gcagaaggta caagtgtagt gactgaaaca gtgtttgaaa atcgtttcca acatttagaa
     1501 gagatgcgcc gtatgaacgc tcatgtcaaa atcgacggaa atgtagcaat catggatgga
     1561 aaccatgaat tgcaaggtgc agaagtatat gcaacagact tgcgggcagc agcagcatta
     1621 gttttagctg gcttgaaagc aaatggaatc acacgtgtgc gtaacttgaa ttatctagac
     1681 cgtggatatt ataatttcca tatcaaacta cagcaattag gtgcagacgt tgaacgtgta
     1741 gacatggatc aaacttctgc tgaaaaaaca gcacaaacga ttgcttaagg aagtggctga
     1801 atgagtaacc aacgatatat catattaact ttgataaaga ttttagtggt gatcttattg
     1861 cttatccttc tttttgtggc cggaacgatg atcggatatg gtgtcatcgg tggcggcaat
     1921 ccatttaaag tattccagcc aagtttgtgg attcatattc gagacttttt tcattgataa
     1981 ataagaatca cagtggttct agtcgatatg aagtgagcca tgaagcagaa catgacaaaa
     2041 gaaaacagaa gggaacgttg acgttagtcg cgatcccttt ttttagtaaa agaaaagaag
     2101 gaatctaatt gatgaagaag atattgattt atttagttag aggatatcag cgctttattt
     2161 cgcctgcatt tccacctagc tgccgttatt acccaacctg ttccaattat atgataaaag
     2221 caattcaaac ccatggtgca ctaaaaggga cggtaatggg gactgcacgg atcttacgct
     2281 gtcatccttt tgtcaaaggc ggtatcgatt atgtaccatt gacattccaa ttaacgcgaa
     2341 ataagaaaga taaaaaagaa ccagattata aaaaggttgt gaaaaatacg agtagcactg
     2401 agcgataaag aaacaatttg gaaaatagct tcttatattt tgcaaaattg tgagttattg
     2461 ccgaagtgtt ggattttgaa caccgtcaaa aaaaggttgt gaaaaatacg ggtagcactg
     2521 agcgataaag aaaaagagga aaattattgt caaagtaaca actccgcaac tacttatatg
     2581 ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc ttgttattag
     2641 atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt ggctggatat
     2701 ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa aaagaatacg
     2761 aaatcgtcaa tgtagcagta gccccaacgt atcaaggaaa ggggattggc ggaaaattac
     2821 ttgagacagc ttttcagaag ctttcacaac tcgcttccag tcaaactcag atcatcatac
     2881 gaaccggcag tactagcagt gcggcgttac atctctatca aaaaatgggc tatgtggaga
     2941 ttggtcgagt caaagactat tttatccatc attactctga gcccattttt gaaaatggag
     3001 atcaattaag ggatcaagta attttagcaa ttcacttacc taaaaatagc tttaactaat
     3061 aaactttcaa ttcgagaaaa tgctttcaaa tgctaaatag atacattaca atagctttag
     3121 caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt ggatcggctg
     3181 tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg
     3241 aatcttggga agaaatgaaa gcgcaattat ttcaagttta taaaaaaata ctcgtttctt
     3301 ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag aaacaggtga
     3361 ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga gatttggaaa
     3421 ctctgtttca actcccagta acgattgaaa atgacgccaa ctgtgccgga atggctgaat
     3481 tttatgaagg tgctgcccgt gattatcaag atgttgcttt tgttgtggca ggaactggga
     3541 ttggcggggc gttgttcacg aaaggacaaa tcaataaagg ggcacatctt tatggtggag
     3601 aatttgggtt gatgttctta aatggcgata agacattcag taaacttggt acagcggtac
     3661 agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaattcttat accggaaaag
     3721 atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt gagagttttt
     3781 atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca gaagtgatcg
     3841 tattaggcgg cggtgtttct gcaaaagaag ggctcattga tgagatcaaa agccgaatga
     3901 agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta ttatgtgaat
     3961 acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta cgtcaagggg
     4021 agaattaaga gctttagaga aggtgcagct tgcataaaag aagtgaatga aatgaatagg
     4081 agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct gagaggtgaa
     4141 aatgatgtca aaaaaaaata aagatgttga agtgcgtata gaagaagtga aaaaagatat
     4201 caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga ttggtgagat
     4261 cttagcctat gggccaaaag aatatgaaat tttctttggt gaagaagatt ttggaaaaga
     4321 aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc acgaataaaa
     4381 aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag gtattataac
     4441 taagtcaggt tgatacaaac ctttcggagg agtagcgaag tggctaaacg cgacggactg
     4501 taaatccgtt ccttagggtt cagtggttcg aatccactct cctccatttc attggggtat
     4561 agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc
     4621 ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tctttttttt gctttgctac
     4681 cttgttcctt gaagattctg ctgatttaag atagagttaa gttaactaca agaattggag
     4741 ggatccacat gaaatggaaa gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg
     4801 tcaaatcaga aaaatacaat attgaagtga atattccgaa aaaaggcgga aaagcggtcc
     4861 aggtaaaatc aaaacgccca acgaatcata caaaaaaatg gatggctaaa aaccgctaat
     4921 aataacaaaa gatccataag tcggcatttt catcgatttt agggtctttt gttattttct
     4981 aggttgaaca tatctaaaaa attgtttctg acaataggag taggaagaac gatgtgtgtc
     5041 taaatcctct gttgatggat agaaaccttt atctttttca gttgtgaatc aggtatatac
     5101 cagttataat agaagaaacg aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat
     5161 atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag
     5221 accgtcttcc ttcagaaaga gaattagcag tgaaattcaa tgtcagccgt atgacgttga
     5281 gacaagcaat tcaaaacttg gcagatgaag gaatcttaga aagaaagatc ggttcgggta
     5341 catatgttgc gcgagaaaaa gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa
     5401 ttatggaatc gcaaaatcga gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc
     5461 caagctcaag tgaaatggaa aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg
     5521 aacgtattcg tttcgcagat gatgtcccta tttgttttga agttgccagt gtgccacaaa
     5581 agctgatcga tggctacagc aaagcagaga ttactgaatc gttgtatcgc acgctagaag
     5641 aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat tactgctgtg ttggcttctg
     5701 agaaaattgc tgactattta gaggtaaaac gaggagatgc agttcttcgt ttgcgccaga
     5761 tttcttattt tgaagacggt acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc
     5821 gttttgagtt ttatttggaa aaataaaaaa caggtgatcc ctgattcctt acttagaaat
     5881 aaaagagaat cagaatcacc tgtttttata tatgatcagt taccagttgc tcgtgataag
     5941 aaacaggacc ctcttcaaat tggacttgtc cgttcagcag ctcaatgatg gattctttaa
     6001 agtggtcagc acttttttca tctacagcac agatgacgct aacttgatca gtatattgga
     6061 tttcttttat aaaaaaatgt tggagttcaa gataattctg taattttcct agttgcggat
     6121 acgtgatcat cacagagatc tcctgttgca aggtaccttc tacgattcca atgtcagaca
     6181 atgcttgaga gacagcatga gtgtatgctc gtattaatcc gcctgtgccg agtttcgttc
     6241 ctccaaaata tcgagtgact actgcagcta cattgatcag ttgatttttt ttgaggactt
     6301 ccaacatcgg tacgccagcc gttccacttg gttctccatc atcgctgctt cgctggattt
     6361 catttttttc tccaatgatg aaggcgctgc agttatggtt cgctttccaa tgttcttttt
     6421 ttatttcttg aatgaaactt tttgcttggt cctctgattc tattcgtcta agagaacaga
     6481 taaagcgtga ttttttgatc tcaatctcac tttgtccatt ttcttttatt gtacgatagc
     6541 gctcgatcat cttgtcacct ctttttttca ttttctttat tcagtataaa taaagttcta
     6601 ggttgtttca acactgtaac ggttgcgcaa tcgctgtagt atgcgataat aaagacaaag
     6661 gtaggtggat ctggggatgc aggaacgaat gaaaaagtac gatgcaatta cgcattattt
     6721 gaagaacaat ggcggctctc aagtaacgtt gacctttacc caatttgacg aactgctgtt
     6781 tcccagtaac ggacttccca aaacagcaag ggaaagtaca gattggtggg caaatgatta
     6841 taaacaccct gaaaaaggtg cctatggctg gataaatgca ggctatgaag tagttgtcat
     6901 caatttggac aaagaatatg tcgtgttcaa taaattagtg aaatctagtt ggctatttga
     6961 ttaaataaac cgcgtttaga aaacgttgac aaatttcttt ctttttatat aatagagtaa
     7021 cagacgcgaa gaaaacgttt gcgtagtttg ttttaaaaag gagagaaaag tatggctaaa
     7081 aaaacaatca tgcttgtatg ttcggctggt atgagcacaa gtttactagt aacaaaaatg
     7141 caaaaagctg ctgaagcaaa aggaatcgac tcggatattt tcgctgtgtc cgcttctgac
     7201 gcagataaca atcttgcaaa taaagatgta gatgtactgt tattaggacc tcaagttcgc
     7261 tttatgaaag ctgattttga aaaacgtctg gaaccaaaag gcattccttt agacgtcatc
     7321 aatatggctg attatggtat gatgaatggc gaaaaagtgt tagatcaagc aatcacgctc
     7381 ataggtgaat aaatagaaca gtataactat cgaacaagcg atccaaaaaa tagatcgctt
     7441 gttttttcgt atattttttt agggcgaata atatgaatca tttttggggt agaaggcttc
     7501 tgacgagaga tattaaagca atagactgtg aggaggtaaa tttaccaaat tatgagaaga
     7561 tcgcagcagt ggaacgtttg ggcaactgga ttctttgtca tcgctgtgga atgaggacgt
     7621 ccgtcattga tggacagctt gcagattacg gttatttttg tacacattgt ctatatttag
     7681 ggcgatgcga cactcaacaa gatttatatt tgtttgatca gcctgaagca aaacctcgag
     7741 aagtcgtctt tttttggaca ggacagctta ctgaaaaaca aacggaaatc gcaaagagaa
     7801 tactttgcca ttcagaaaaa agacatcatc ttatttgggc tgtaacaggt gcagggaaaa
     7861 cagagatgct ttatcctatt ttagtggaaa ctttaaaagc tggaggaaga gtagcgattt
     7921 gtacaccgcg agtcgatgtt tgtaatgaat tgtttttacg ctatcgttat gtttttccag
     7981 aagaaaaaat cacgttgctc catgggaata cagcaacggc ttataccttt acttcttttg
     8041 ttatctgtac gattcatcag ctataccgtt tttatcggtc ctttgattta atagtgatcg
     8101 atgagatcga tgcgtttcct tataacgggg atgcaggatt agctcatgct gtgcaaacag
     8161 cgattaaacc agacggacga ttcatctact taagtgccac acctgataaa aagttactaa
     8221 aggaaatcaa gcagacgttt gacatccaca aacttgcttt gcgctttcat cggcgtttac
     8281 ttcctgaacc cgtcttggtt ttcatgaata attggcgaca aaaatgcaag aacaagaaga
     8341 agataaaatc tttggtgcgg atcattcatc aattaattaa agaaaaccat atccttttat
     8401 tttgtccaag tatcacgatg atgcatcgtt tgaaagccac acttcaagag gctcttccga
     8461 gatatgagat cacagatgtt tcttcaagag acaatcagcg ggcggaaaaa gtccagaaaa
     8521 tgcgagaagc aaaatatgac atactgttaa ccacgatgat ccttgaacgt ggagtcacat
     8581 ttgaaagaat atccgttatc gttttaggag cggatcatcc ggtttttact aaatcttctt
     8641 tggtacaaat cgcaggaaga gccgatcgta aagggcctta tacgaacagt caagtatact
     8701 ttttctatga agaaaaaact gcagctattc gaaaagcttg tcaggaaatc aaagaaatga
     8761 atgaagaagg gaagaagatg ctgtgaggtg cagttgctgc agtaaagata tcgtccaaaa
     8821 tttaacattc agcgagatta ttggtgtttc gaaatacaga ttacctcaga cttgttcagt
     8881 ttgtcgcgca caatttcatt tattggaaac aagaggctgt tctggctgtc aaaaaccaat
     8941 ccatttcaat ctagagagta cgaaaaagaa agtaaagtct attcccgttt actgcagtga
     9001 ctgtcaagaa tggaagaaga aatatccgac ctatgatttt cagcaccgtg ccttttttgt
     9061 gtacgatgaa aatatgaagg cctggctcaa tcaatacaag tttatgggag atatccgact
     9121 agcaggcacc tttgcatata aatggacgca gataaaaaaa gaatacccga catacatcta
     9181 ttgtccaata cctttatctg aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga
     9241 gatgttgaaa gcagcaagaa ttccttttca gatgctactc aaaagagaga agcatctgct
     9301 ggctcaagca aaaaacacgc gaaaggaaag attggagatg cctcaacctt ttgaacttgc
     9361 agtcaccttt tctaagatac aaaataaaaa aatattgatt attgatgatg tctatacaac
     9421 aggacagact cttttccacg cagcagattg tttgcaggtc gcacagccga aaatcatccg
     9481 aaccatttct ttagcaagat gaattagatg tggaaatttg atagcgattt aatttgcaaa
     9541 taacctagag ttcgatataa tagaattaag aagagggaaa gagtggtcct tctccttctt
     9601 gattgtggta gacgaaaggg gtaattttta tgtttagata taatgtacgc ggcgaaaata
     9661 tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct
     9721 atttcagtga cgcacccgat gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa
     9781 cagcaaaagt ggaagtaacg atcccattac cttatcttgt attacgtgcc gaagaaactt
     9841 ctccagattt atacgcaagt atcgatttgg ttgttgacaa attggaacgc caaatccgca
     9901 aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg catgaatact gcagatgcta
     9961 ctgctttatt caatgaagag gacgaaaacg aaaatgattc tgaattagat atcgtacgta
    10021 cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc agtcttacaa atgaacatgc
    10081 tgggacataa cttctttatt tttgaagatg cagaaacaaa tggtacaagt atcgtttacc
    10141 gccgtaaaga tggaaaatac ggactgattg aaacagactg atcagcctta cttaaataac
    10201 aacgaaaact tgcagcagct actgacagct gccgcaagtt ttt
//
LOCUS       DASHFF010000086        10242 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.086, whole genome shotgun sequence.
ACCESSION   DASHFF010000086 DASHFF010000000
VERSION     DASHFF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.086"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            71..757
                     /locus_tag="U2I75_002472"
     CDS             71..757
                     /locus_tag="U2I75_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN1697552.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            757..1917
                     /locus_tag="U2I75_002473"
     CDS             757..1917
                     /locus_tag="U2I75_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1697553.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            1927..2802
                     /locus_tag="U2I75_002474"
     CDS             1927..2802
                     /locus_tag="U2I75_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697554.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            2799..3110
                     /locus_tag="U2I75_002475"
     CDS             2799..3110
                     /locus_tag="U2I75_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697555.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            3100..3453
                     /locus_tag="U2I75_002476"
     CDS             3100..3453
                     /locus_tag="U2I75_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1697556.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            3443..3844
                     /locus_tag="U2I75_002477"
     CDS             3443..3844
                     /locus_tag="U2I75_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697557.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            3837..4241
                     /locus_tag="U2I75_002478"
     CDS             3837..4241
                     /locus_tag="U2I75_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697558.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            4253..4861
                     /locus_tag="U2I75_002479"
     CDS             4253..4861
                     /locus_tag="U2I75_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1697559.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            4881..5243
                     /locus_tag="U2I75_002480"
     CDS             4881..5243
                     /locus_tag="U2I75_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697560.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            5445..8864
                     /locus_tag="U2I75_002481"
     CDS             5445..8864
                     /locus_tag="U2I75_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN1697561.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            8915..9652
                     /locus_tag="U2I75_002482"
     CDS             8915..9652
                     /locus_tag="U2I75_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1697562.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            9662..>10242
                     /locus_tag="U2I75_002483"
     CDS             9662..>10242
                     /locus_tag="U2I75_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697563.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLG"
BASE COUNT         3636 a         1532 c         2161 g         2913 t
ORIGIN      
        1 gataaggcga tggcagatga aatatttata actaaaaaca atcaacaagt atcgaaagga
       61 ggtgaggcga atgacgacaa tgaaaacatt tctagcagta aagaatgaag gcgcagtacc
      121 gcaaattttt attcagggat ttattggttc tagttggttc tttgaaggga atactgacaa
      181 gggaatcaaa aatattttgg atagtctagg tgatcaagaa gaaattgaag tagtaattaa
      241 ttcaaacggt ggagacgtat ttcaagggat tgctattggg aacttactta agtcaaataa
      301 agcaaaagtt aacgttgtga ttaacggctt agccgctagt gctgcttcaa ttatcgcaat
      361 ggctggcgat actataaaaa tctacaacaa tgcacaattg atgattcacc gcgcttccac
      421 atacggagaa ggaaatgtcg atgacttccg tacgattgct gaccaactgg aatcaattga
      481 taaatcggta aaggcttcat ataaaacacg attcaatggc acagatgaag cattgcaaga
      541 acttcttgaa aaagaatcgt ttatggatgc agaaacagct ttgagttatg gattggtcga
      601 tgaaattatc gatgcagaaa atagctcagg tactgaagct aaaaaagaac aaagcgttga
      661 agaaattttg aatgacgtta aagaaaaaag agcagaaaaa attgctgcat ttacagcagc
      721 attaaataaa acatttggac aaggagatgt aaaataatga cagttaaaaa tttaaaaggt
      781 gtaacagctg caagcgacca attgatgaaa gcttttaaag atggtaacga agaatctttt
      841 agcgcagcta tggtaagctt atctaaggaa attcaggata aaattttaga agaagcaaca
      901 gcaaaaaatc aagatcaatt agtattaatg aaccgtggtc agcgtgtatt aactacacaa
      961 gaaacaaaat tctataacga agtagtgaaa aacgaaggtt ttgcaggggt cgaagaatta
     1021 gtgccagcta ctgtatttga acgtgtattt gaagatttag aacaatctca tccactattg
     1081 caaaaaatta cttttgttaa cacaactggt gtaacagaat ggattgtgtc acgtggagtc
     1141 aatccagcat ggtggggtaa actttgcgaa gctgttaaaa aagttttaga taatggcttt
     1201 gatgtaatta acatgaagca gttcaagcta tcaggttata ttcctgtatg taaggcaatg
     1261 cttgatttag gtccagtatg gttagatcgt tatgtccgta ctgttttagt agaatcattg
     1321 agaattgcat tagaacaagc aattgttgat ggtactggta aagatatgcc agtcggaatg
     1381 atgcgtgaca tgagcaaaca aactagcgga gaatatgctg aaaaaaaagc agaacctatt
     1441 acagctttag atgctgtaac tatgggcggt ttgatggcgc gactatcaaa attcaatatc
     1501 gaaggtgtga atgatccgat ttatcgtaat gtgaatcctt ctgatgtggt cctaattgtg
     1561 aatccaactg attactggtc taaagtattc ccagctaaga ctgtactaac tgctaatgga
     1621 gaatacgtac aagtattgcc agtaccagtt tcagatttgc agtcaacggc tgtgccagaa
     1681 ggaaaagcag ttattggggt agcctcagat tacttcatgg gtgtaggatc tacactaaaa
     1741 attgaagctt cagatgaata ccattttgtt gaagacgaac gcatttatct agctaaacaa
     1801 tatgcaaacg gtcaacctaa acgtaacgat agtttcattg tattagatat tagcgctttg
     1861 ggaactacta ctacaactac aaaaccaaca accacaacaa ctacaacaca agcgtaggtg
     1921 atcagaatga agtatattct ttgtcagccg gcaatcaatc ggtttaaatg ggagcttgaa
     1981 gtttgtttaa ctaatctgaa gaaactagga atcaaagata tcgtattgct tttcagcaga
     2041 cacgatgatc agattcctat tttttttgag aaggaatatg gtgttgaagt tcatgtgtac
     2101 gatgatctgc gggacgacaa agagtatatt ccttcgatta aaccatattt atggtggaaa
     2161 tatttagaag aagatcattc gcgtgaggac gaccgatatt tctatatcga ttcggatgtc
     2221 attttcaata aaagaattaa tttgcgcaaa ttgccttcta aagatgatgt ttggtattgt
     2281 agcgactgct gtagttatct aagtcttgat tatattagaa gctgtgaaaa cggagaaaat
     2341 attctaaaag atatggcaaa cattgtaaat gttacagtag aatctttgga aactataaac
     2401 actaattcag gaggcgcaca gtgggttatt aaccgcccta aagcgaatta ttggaaaaag
     2461 gtttatctgg attctaatcg gctatatcgc taccttagag ggcaaaaaac aaatatacaa
     2521 atctggacag ccgagatgtg ggcacagctt tggaacatga tgtatttcaa tattggtcct
     2581 aaagttcacg aggaattaga cttttgtttt gctactgatc caatagaaaa agttaaagaa
     2641 gtaaaaatct tacacaatgc tggagtaaca acaaacgatg aagatttatt tttcaaaggg
     2701 agatacgtga cttctacgcc ttttgatgaa gatttatcat ttgtaaacaa gaaaaaatgc
     2761 tcttacgcat atgttaaagc aattaaggcg gtggttagat gacgcctgaa caagtgactg
     2821 aagaattgct aatagctgtg aaggataata tttacgttac ctggaacgaa gaagatgagt
     2881 caattaaaaa gatgatagct aaaaatgctg tttatcttca aagtaaagtg agtacaacac
     2941 tttctttttc tcctgaaagc ttagaatacg gattgctaat cgaaagatgt agatacgact
     3001 ggaatcgtgc tttagatgag tttgaacaaa atttcgctag tgagttatta ggtttcattc
     3061 aacattatgc gctacaagaa tatattgcag gtgatgtgaa tggcgaataa tcgtagactc
     3121 gaagaaacat tcaacgatgg ttggttaaag attttgacgc aaactaccaa aagaaatgaa
     3181 ctaggaaaaa agattggtgt agaagataca gaaatcactt ctttaaaatt tagaaatctt
     3241 tccatgagag atagtgatat aacagctatg gatgcgatgg gatcgaaatt aactaagaaa
     3301 gtaaagactc catttcatcc aatcgccaag aaatttaata aagatcaata ttttatcgta
     3361 atcgatagta tgcgttacaa cgttatctat gccgattacg ataattttta tatctatttt
     3421 tatcttgaaa gtgtgggtga atatggtgat tgataattct aaagaaaaag aacgtttaaa
     3481 taagcaaatt tctgctatca aaacttcctt agaagagcat tttggcctca aactctttca
     3541 agactccgta ggcgaggatg agctacctga tgattttaat tacttcattc tcgaaacagg
     3601 agaaatagaa atgatcactg agccaaaata tagcgtgggt caaaatctat atctaacttt
     3661 ctattcagaa aatagagaag atttaacagg agactcacta gatattattt cattgattca
     3721 aaatcgttcg attcgttttc agagaatgga tcccaatcat ttaaaactag agaaccaaga
     3781 tcgctatatc gatcaattgg tatttacgtt tagacgatta ttgaagagtg attgtcatgg
     3841 ctaaaaatag ttgggagcta aaaataaatg gacatgatga acttcttgtg cggatggaac
     3901 gctattcaag cgagagcgaa cgactgatta acgaagcatt gaaatcaaaa ggttcggcta
     3961 ttgcagtgga taggattaca gaaaaaattc ctgtttctga agcagattta agaagagggc
     4021 accaacacgc aaaaaatagt cgtccactta agactcaata cattaatttg ggtttcatca
     4081 ttagacctac aagaaaattt gagtatttaa aatatcctga tttggggata ggtacttcta
     4141 aaagaaatca gccggacgaa tttatgagaa gaggattagg tcttgcactt gatccaatta
     4201 cagaacttct gattcgtcaa ttcgataaat taaataaata gggggaacaa caatggctaa
     4261 aacaaaaact gtagtaacaa cgttcgataa cgtaagtatc aaacgaattg cttttaattt
     4321 taagaacgca ggaaatgcaa tcgcaacgaa ttgtaatgga caattagatg gcgaaacaga
     4381 aatgcaaacg gtggttaaaa aatgtggagc gacagaagta aaatcaaaat ctaaaccaat
     4441 caatatgacg gtaacaatta ctgcacatgt accgatggaa gtttatcgac gtttcaatgg
     4501 gttgaaacaa gatggacgta ttaaaccagg catttactct tacggtcctg attccgtagg
     4561 cgaagatttc tcacttgctg cagagatcgt ggatgacttc gaagaaaata gaaagttagt
     4621 tggtatgtta gcatgcactt cgaatacagg attaacattc tctattgaaa atggtgcgga
     4681 tgaagtagct gcgttagaac tagaaacaaa agttatgcaa gatgaatttg gtaaatttta
     4741 tcatgaagca attgttgcag aacttgaaga agacttaaca gatcaatgga tgacgaatct
     4801 atctgctgat gtgattaaaa aaacttcagt tgtgacaact acggccactc aatcacagta
     4861 aaaaaaacgg aggtagcgaa atgaacgaag attactcaaa aattgaacta aacgatggaa
     4921 caattttgaa tttagaacct aaactgaata tcaagaaatt attgatgatc aatagagatt
     4981 ttaacacaga cgagtttgca aaaatgactg tgggaaaagg atccatggat atttctgtta
     5041 ttcaaggtgc aaaggctgtg tatattgctt accgccaagc gaacatgact gattatattt
     5101 cattcgatga atttatcgat aaatgggatt ttgatatggc tactgccagc tatatttatc
     5161 aattgatgat gttcaaacaa gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata
     5221 aggaaaaaaa gcttcaaaag taaaaattcc aaagctctta gttgaaacgt gggtcgatgt
     5281 ctattcgatg ttgaccgacg ttttttctat gccttcagat ttggttttaa gcgatatctg
     5341 tttagatgac attttgcaaa tggcttacaa caagagtgct tatgaaggat ggaaaaacta
     5401 tgcaataaac caatcccaga aaaactaaag aaaggaggta aaaaatggct aaaaagagaa
     5461 cagaagcaga agtaactttc atagctaacg atgacggatt gaaatctacg ttaaaagaaa
     5521 tcagtgctga attaactaaa aatagagcag aattaaaact agaacaagct caattacaac
     5581 agactggttc tgaatcagac aagttaggaa gtaaattatc ttctttagaa aagcagtatg
     5641 aattacaaag tcaaaaagtt gaagtaacta gccaacgttt agccaatgcg aaaaaatatt
     5701 atggagaaaa ttccaccgaa gttcagaaac ttgaaagaga actgattaat caacaaacag
     5761 cgcaacaacg tttgtcaaac gaaattgata aaacgagtaa tgcactagct caagcaaaag
     5821 gcgaaataca gacgtacgag tctacaatgc aacagttgga tagcgaacaa aaaaatgttc
     5881 aagctagtgc ttctctgatt gaatccgaat acaaaaaatg gcaagcaact gctggtcaat
     5941 cagcttctga agccgagaaa ttagcgaaag cccaagaata tgtttctcaa caatctgaaa
     6001 atgcggagaa aacgatagat atcctaagac gacagttaga agctacacag tctgaatttg
     6061 gcgctacatc cacagaagca atgcagatgg aagcgaagct taatgatgct gaacgtgaat
     6121 ttgaagagtt aggacaagct gctaaaaatg tagatacaac taacttggac gatatcggaa
     6181 gcaaaataga tatgaataat ttaatggaag cttctgacgt tttaagcgac attggcgata
     6241 agcttacaga attagggaaa caagcagtgg actctgctaa cagtgtaggt agttcccaga
     6301 gtaagataca agctaatttt ggtttgtcta aacaagaggc tgaagaatta acgaatgtag
     6361 ccagagacat ttattataaa ggttttggag aatcgttaga tcagtccaca gatgcattga
     6421 ttttggtaaa gcgtaattta ggagatttaa ataatcaaga tttacaaaat atcacggaac
     6481 aagctatggt cctagaaaac accatgggcg ctgatatgga tgaaacgtta cgtggtgtaa
     6541 atggcttaat ggtcaatttc ggcttgagtg ctcaagatgc aatggattta atggtttcgg
     6601 gtactcaaaa cggtttagat aaaacgcacg aattaggcga caatatggca gaatatagcc
     6661 aattatggag tcaaatggga tattcagctg atgaaacgtt cggaatgctt caaaatggtt
     6721 tagatgcggg tgcttataac cttgataaag tcaatgactt agttaaggaa atgggaatat
     6781 cgttaacaga tggtcgattt gagcaaaaca tggatatgtt tagtgaaagt actagaaaag
     6841 cttttgaaga gtggaaaaat ggcggaggaa cacaaaaaga cgttattaat tccatgattc
     6901 aagattttag caatatggat ggtcaatacg accaattaaa taaagcttct acaatttggt
     6961 ctgcacttgg cgaagataac gcgatgaaag ttgtccaatc tttgactgat gttaaccata
     7021 catttgatga tgttagtgga tctgcacaaa aaatgaatga agattctact actccgttac
     7081 aagaattaaa cggaaaaata gctgaattaa aggattcatt agctcctata ggcaacacaa
     7141 tcatagatgc actcgaacca gtaattgatt ttctaggaaa gatggctgat gcgtttaata
     7201 atcttccaca accagtacag gattatgccg tagcgattgg cggattgact gctgcattta
     7261 ctttattaat gccaataata gttggcttca tggctctagg tggtcctact acattaataa
     7321 taggagcagt tattactgtt attgctggag ttatagcaat tataaaaaac tggggcgcaa
     7381 ttactgactg gtttaaggga atatggagta aattcactga ttggttgggt ggtacttggg
     7441 aaagtataaa agaaggtgcc tcatcagttt gggatggagt taaagaaacc tggtctggat
     7501 ttgtagattg ggttcaagat atttggcaag gagtttctga ttggtttgga gagctatgga
     7561 gcggattagt tgaaggagct tccaacatct ggcaaggagt ccaagagact tggcaagcat
     7621 tcgttgattg ggtttcaaat atttggaacg gagtcaaaga agtatggtcg attatttggg
     7681 cagacattgt aggaattgtt caaataccat ggaccttaat aacgtcattg attcaagccg
     7741 gtattaatat tatcgtgggt atttttgatg tagctggaca gttattaggc gcagcttggc
     7801 aagctgtttg gacacctatt tctgatttcc ttaaaaacac ttgggatact atgacacaat
     7861 gggtaagcat cgcttggaat ggaattgtaa ctacattcca tactatattt gatccagtag
     7921 tggcatggtg gaatggtata tggacagcta ttagtactac ggcttcaaat atttggaatt
     7981 caattagtgc aacagcttct agtatttgga acagtatcaa gaatacaatc actagcttgg
     8041 tacaagcagc tgctacagta attcaaaata tttggtcaac tgtatctagt tggttaggtg
     8101 gaatttggaa ttcaatcagc tctacagcat caaatatctg gaatagcgtg actagtagta
     8161 taagcaatgc tataaacgca gctaaaagtg ccattcaaag tgtttggaat agtatatctt
     8221 cgtggatcag cggaatttgg aacggtatca aaaacactgc tttgaatctt tggaatggaa
     8281 ttacaagcac tattagctct aaagtaaacg atggaaaaaa tgcaatttca agcggttggt
     8341 ccaatctaac aggtattgtt tccgacatat tcaataatgt taaaagtaca attgctaaca
     8401 tttgggaagg tatcaaaaag actgttagcg ctccaattga ttggatcaga gataaaatca
     8461 gtagtatctt tgataatttg aatatttcta taccacatat tccgttacca cattttaaat
     8521 tgagcggaga attcaatcca ttgaagggga aaatcccaac gttgggtgtt gattggtatg
     8581 cgaaaggtag tgtgtttaat tctccgaata ttatcggtgt cggcgaagca ggacctgaag
     8641 cagttttacc tttgaaaaga tctgtgctgc aagaaattgg tgatcgtatc ttgagtagca
     8701 catcagtttc atctagggca caaacgattc aacctgtgaa taactacgaa ttcaatttca
     8761 caattgatgg taacgcagat gaggttacta tgaagcaaac aactcaacaa atcattgata
     8821 gcattacaaa agttcaaaat gataatgctt cggcatggcg ttaaacagga gagtatttct
     8881 cctgtttttt tagtattaaa aaggatgtga aaaaatgact gattgtatac attctataat
     8941 cgatggattt cctgattatt tgcataaatt ggctttagct gaaagaccaa ccataccttc
     9001 tccaaaaaga cagagagttg aaacttctgt tttaggaagg ttaggtggct tagtacaaga
     9061 ttactcgttt gaagacatgt cgtttacatt gcactataac tatttagagg atgtggaaga
     9121 ccatcaagcg ttcaagcaat cgttttatat catgcgtcat tggttaaatt atgcaaagaa
     9181 attagaattc tctgatgatc ccaacgtcta ttatgttatc cagactatcg atattgggga
     9241 tgcagaaaac gatattgttg aatggggaga gttcgatgta aatattactg cgaaaccatt
     9301 cgcaagagtt caagaagatg tacctataac cgtagataaa ccacagtcat ttaacttgct
     9361 gaataatagt ttagaagaaa gttttccaaa gattatcatc actccttcag ctacttcatg
     9421 ccagttcatc ttaaatgatt atgtgtttag ttttgaaggc ttagtagtag gaactgacgt
     9481 agtcattgat agtgatttga tgctttgcta cgaagagcaa tcggacggag atattttaga
     9541 tcggtccaac aaaatgaaga ccatgcaata tccgacattg caagtggata ttaattattt
     9601 taattgtact ggtttgagca aaatacaaat ttatcgtaat gggttaaggt aggtgaaata
     9661 gatgatcgat aatttaataa ctatttacga taaaaacgac gcgaataatt tagctgaaca
     9721 tttatatgat acgcaaggtt taggcgcttt gtcagactgg ttaacagcta ctgttagcaa
     9781 taaattaaac ggagccgaga tatttcaggg tacttatcca ataagcggaa ctaatgcaga
     9841 tttgattgta gaaggacgta ttattcagtg ttatgtagat gaaaatcgag caaaacagcg
     9901 tctacggatt tattatgcaa agacttctgt aataggcaat acgatagaag taaaagctga
     9961 acctattttc aatgatataa gaaaatcggt gttgaataaa tatgacagtg gaacagaaaa
    10021 gatcactgct agtcaggcat ggcaaaacgc aaaaacttta gcgaaaccag ttattccttc
    10081 acagttttct ttctcgtcat tagtagatac gcttgctaat gtgaagatag aaaaggcgaa
    10141 ttttttagaa ttctttggtg gaaaagaggg atctattcta gatcgatttc atggggaatt
    10201 tctaaaagat aataacacat tacgtcatga aaaaaggcta gg
//
LOCUS       DASHFF010000087        10022 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.087, whole genome shotgun sequence.
ACCESSION   DASHFF010000087 DASHFF010000000
VERSION     DASHFF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.087"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..722)
                     /locus_tag="U2I75_002484"
     CDS             complement(<1..722)
                     /locus_tag="U2I75_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1697564.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="U2I75_002485"
     CDS             complement(727..1494)
                     /locus_tag="U2I75_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1697565.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1983..2408
                     /gene="lacA"
                     /locus_tag="U2I75_002486"
     CDS             1983..2408
                     /gene="lacA"
                     /locus_tag="U2I75_002486"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEN1697566.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2425..2940
                     /gene="lacB"
                     /locus_tag="U2I75_002487"
     CDS             2425..2940
                     /gene="lacB"
                     /locus_tag="U2I75_002487"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEN1697567.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2951..3883
                     /gene="lacC"
                     /locus_tag="U2I75_002488"
     CDS             2951..3883
                     /gene="lacC"
                     /locus_tag="U2I75_002488"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEN1697568.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3886..4866
                     /gene="lacD"
                     /locus_tag="U2I75_002489"
     CDS             3886..4866
                     /gene="lacD"
                     /locus_tag="U2I75_002489"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN1697569.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4894..5211
                     /locus_tag="U2I75_002490"
     CDS             4894..5211
                     /locus_tag="U2I75_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1697570.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5211..6917
                     /locus_tag="U2I75_002491"
     CDS             5211..6917
                     /locus_tag="U2I75_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1697571.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVVSNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7059..8465
                     /gene="lacG"
                     /locus_tag="U2I75_002492"
     CDS             7059..8465
                     /gene="lacG"
                     /locus_tag="U2I75_002492"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN1697572.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8588..8761
                     /locus_tag="U2I75_002493"
     CDS             8588..8761
                     /locus_tag="U2I75_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEN1697573.1"
                     /translation="MKLPSLLKCGKWYVLTGKEWICHSDLELSAFEWEFLQSLDVEIR
                     ETIHFEVNYLPFQ"
     gene            8782..9681
                     /locus_tag="U2I75_002494"
     CDS             8782..9681
                     /locus_tag="U2I75_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN1697574.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3164 a         1689 c         2006 g         3163 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt ttttaagaaa acaaataaaa aaaacaaaaa ataaacaaaa aagtgttgct
     1861 ttttgtttga atgattgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     1921 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     1981 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     2041 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     2101 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     2161 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     2221 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     2281 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     2341 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     2401 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     2461 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     2521 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     2581 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     2641 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     2701 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     2761 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     2821 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     2881 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     2941 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     3001 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     3061 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     3121 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     3181 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     3241 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     3301 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     3361 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     3421 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     3481 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     3541 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     3601 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     3661 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     3721 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     3781 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     3841 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     3901 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     3961 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     4021 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     4081 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     4141 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     4201 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     4261 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     4321 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     4381 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     4441 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     4501 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac
     4561 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     4621 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     4681 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     4741 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     4801 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     4861 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     4921 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     4981 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     5041 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     5101 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     5161 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     5221 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     5281 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     5341 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     5401 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     5461 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     5521 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     5581 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     5641 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     5701 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     5761 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     5821 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
     5881 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
     5941 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
     6001 atcttatcca agctggacaa catgcagata aagttatcac ttctggtact caaatgttta
     6061 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
     6121 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
     6181 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
     6241 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
     6301 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
     6361 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
     6421 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
     6481 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
     6541 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
     6601 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
     6661 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
     6721 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccgcaggtcg
     6781 tttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
     6841 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
     6901 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcagtt
     6961 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
     7021 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
     7081 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
     7141 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
     7201 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
     7261 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
     7321 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
     7381 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
     7441 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
     7501 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
     7561 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
     7621 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
     7681 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
     7741 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
     7801 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
     7861 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
     7921 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
     7981 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
     8041 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
     8101 ttatttatcc tgaaggcttg tatgaccaaa tcatgcgatt gaaaaatgat tatccaaact
     8161 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtagataata
     8221 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
     8281 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
     8341 catggtcaaa cggatacgaa aaacgttatg gactattcta tgtagacttt gacacgcaag
     8401 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
     8461 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
     8521 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
     8581 cctgagcttg aagctaccct cgttgctaaa atgtggtaaa tggtatgttt taactggaaa
     8641 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
     8701 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
     8761 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
     8821 tttaaaacac ttggtggtca actgacttcg attaaagacg aggatgggat agagtatctc
     8881 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
     8941 agtttacgaa atgattgggc tatttataga cctcaagaaa gacctttttt tacaggtctt
     9001 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
     9061 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
     9121 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
     9181 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
     9241 tctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
     9301 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
     9361 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
     9421 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
     9481 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
     9541 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
     9601 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
     9661 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
     9721 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
     9781 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
     9841 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
     9901 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
     9961 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    10021 gt
//
LOCUS       DASHFF010000088         9964 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.088, whole genome shotgun sequence.
ACCESSION   DASHFF010000088 DASHFF010000000
VERSION     DASHFF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.088"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            8..81
                     /locus_tag="U2I75_002495"
     tRNA            8..81
                     /locus_tag="U2I75_002495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="U2I75_002496"
     tRNA            101..173
                     /locus_tag="U2I75_002496"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="U2I75_002497"
     tRNA            201..274
                     /locus_tag="U2I75_002497"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="U2I75_002498"
     tRNA            288..361
                     /locus_tag="U2I75_002498"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="U2I75_002499"
     tRNA            385..474
                     /locus_tag="U2I75_002499"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="U2I75_002500"
     tRNA            487..560
                     /locus_tag="U2I75_002500"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="U2I75_002501"
     tRNA            579..654
                     /locus_tag="U2I75_002501"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="U2I75_002502"
     tRNA            656..726
                     /locus_tag="U2I75_002502"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="U2I75_002503"
     tRNA            740..813
                     /locus_tag="U2I75_002503"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="U2I75_002504"
     tRNA            838..925
                     /locus_tag="U2I75_002504"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="U2I75_002505"
     tRNA            947..1018
                     /locus_tag="U2I75_002505"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="U2I75_002506"
     tRNA            1041..1130
                     /locus_tag="U2I75_002506"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="U2I75_002507"
     tRNA            1143..1216
                     /locus_tag="U2I75_002507"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="U2I75_002508"
     tRNA            1235..1307
                     /locus_tag="U2I75_002508"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="U2I75_002509"
     tRNA            1311..1383
                     /locus_tag="U2I75_002509"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="U2I75_002510"
     tRNA            1388..1458
                     /locus_tag="U2I75_002510"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="U2I75_002511"
     tRNA            1471..1544
                     /locus_tag="U2I75_002511"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="U2I75_002512"
     tRNA            1556..1627
                     /locus_tag="U2I75_002512"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /locus_tag="U2I75_002513"
     CDS             1893..3086
                     /locus_tag="U2I75_002513"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN1697575.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="U2I75_002514"
     CDS             3216..4688
                     /locus_tag="U2I75_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1697576.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="U2I75_002515"
     CDS             4771..5559
                     /locus_tag="U2I75_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN1697577.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="U2I75_002516"
     CDS             complement(5626..6294)
                     /locus_tag="U2I75_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN1697578.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="U2I75_002517"
     CDS             6409..8121
                     /locus_tag="U2I75_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1697579.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="U2I75_002518"
     CDS             8124..9896
                     /locus_tag="U2I75_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1697580.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2900 a         1781 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DASHFF010000089         9571 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.089, whole genome shotgun sequence.
ACCESSION   DASHFF010000089 DASHFF010000000
VERSION     DASHFF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.089"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            130..951
                     /gene="racE"
                     /locus_tag="U2I75_002519"
     CDS             130..951
                     /gene="racE"
                     /locus_tag="U2I75_002519"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN1697581.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            954..2309
                     /gene="rph"
                     /locus_tag="U2I75_002520"
     CDS             954..2309
                     /gene="rph"
                     /locus_tag="U2I75_002520"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN1697582.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            2310..2828
                     /locus_tag="U2I75_002521"
     CDS             2310..2828
                     /locus_tag="U2I75_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1697583.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            2935..3435
                     /locus_tag="U2I75_002522"
     CDS             2935..3435
                     /locus_tag="U2I75_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1697584.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(3715..4635)
                     /locus_tag="U2I75_002523"
     CDS             complement(3715..4635)
                     /locus_tag="U2I75_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN1697585.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            4800..5813
                     /locus_tag="U2I75_002524"
     CDS             4800..5813
                     /locus_tag="U2I75_002524"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN1697586.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(5923..6678)
                     /gene="fabI"
                     /locus_tag="U2I75_002525"
     CDS             complement(5923..6678)
                     /gene="fabI"
                     /locus_tag="U2I75_002525"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN1697587.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            6898..7338
                     /gene="fabZ"
                     /locus_tag="U2I75_002526"
     CDS             6898..7338
                     /gene="fabZ"
                     /locus_tag="U2I75_002526"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1697588.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(7453..8214)
                     /locus_tag="U2I75_002527"
     CDS             complement(7453..8214)
                     /locus_tag="U2I75_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1697589.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            8403..9497
                     /locus_tag="U2I75_002528"
     CDS             8403..9497
                     /locus_tag="U2I75_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1697590.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         3108 a         1679 c         2007 g         2777 t
ORIGIN      
        1 aagtcacaac cgcttttcct ctttccattc gaaaaaaaga gatttcgatt gttttaagca
       61 agtaagagca cttgaaaatt cacgctaaaa agtacacaat agggaggata gaatttattg
      121 aaaaaaagga tgatacgatt gacagataat cgccctatcg gatttattga ttcaggtgtc
      181 ggcggcttga ctgtagtaaa agaagccctg aaacaattac cgaatgaaaa tattttattt
      241 gtaggagaca cagcacgctg cccatatggc cctagacccg cggaacaggt aatccagtat
      301 acttgggaaa tgacggatta tctggtggag caaggaatca agatgctggt gatcgcctgc
      361 aataccgcaa ctgcggtggc tttagaagaa atcaaagctg ctctttctat tccagtcatc
      421 ggtgtgatcc ttcccggtac tagagcggca gtaaaaaaaa cacaaaataa acaagttggc
      481 attatcggta cgattggtac ggtaaaaagt caagcttatg aaaaagcact gaaagagaaa
      541 gtaccagaat tgactgtgac aagtcttgct tgtcccaaat ttgtttcagt tgtcgaaagt
      601 aatgaatacc attcatcggt ggcgaaaaaa attgtggcag aaacattagc tcctttaacc
      661 actaaaaaaa tcgatacatt gattttggga tgcacccatt atccattatt acgccccatc
      721 attcaaaatg taatgggaga aaatgttcaa ctgatcgatt ctggagcaga aacagtaggt
      781 gaagtatcta tgctgttaga ttatttcaat ttgagcaatt caccgcaaaa tggtcggaca
      841 ttatgccagt tttatacaac tggctctgcc aaacttttcg aggaaatagc tgaagactgg
      901 cttggaatcg gacacttaaa tgtagaacat atcgaattgg gaggaaaata aggatgcgtc
      961 atgacgggag aaaagcccaa gagcttcgca aactggagct tcaaacaaat gttttcaaat
     1021 atccagaagg atcggtagtg atccgttttg gtgatacaac agttatttgt tctgcaacaa
     1081 tagaaaattc tgttccccct tttttgagag aaacaggaac aggatgggtg acagcagaat
     1141 atagtatgct gcctcgagca acgagtacga gaaatcgtag agaaagcagc aaagggaaac
     1201 tttctggtag aacaatggaa atccagcgtc ttattggtcg ttccttgcgt gcagtagtag
     1261 acttggaaaa attaggagaa cgtagtatca tcgtagactg cgatgtgatc caagcggatg
     1321 gcggtactag aacagcaagt atcactgggg catttgtggc attgaaatta gccattgaaa
     1381 aacttttaag agaaaaagaa ttgtcagaag atccaataaa agaacatctt gctgcagtta
     1441 gcgtaggaat cttaccagat gggacttgcg tcacagattt ggattaccaa gaagattccg
     1501 cagcacttgt agacatgaat cttgtaatga cagaatctgg aaaattcgtg gagattcaag
     1561 gaactggaga agaagcaacg tttgatggtg aacagttgaa tgaaatgctc ttttttggaa
     1621 aaaacgcgat tgaagatttg atcaaagagc aaaaacatgc tttgctaaca gagtttgctc
     1681 aaaatgatga gcggatagaa gaaacaaaaa caatcatcat tgcgacaaga aatccaggaa
     1741 aagcagaaga gtttcgaaat atgttcaaag aagcgggata tcatgtaaaa acattacttg
     1801 actatcctga attgccagat gtggaagaaa ctggcagcac atttgaagaa aatgcccgat
     1861 taaaagcaga aacgattgca caattgcttg atcagccagt cttagcagat gattcaggtt
     1921 tgaaagtgga tgctttaggc ggtatgccag gaatctattc tgcacgtttt gcaggggaac
     1981 aaaaaagtga tgcggggaat aacgccaaat tattatatga attgacagat gtgccggatg
     2041 aaaaaagaac agcacaattc cattgtacgt tagtattcgc ggcacccaaa aaagacagcc
     2101 ttgtagtaga agcggaatgg cccggtcgag tagcccgtat accatctggc gaaaatggct
     2161 ttgggtatga tcctttattt atcccagaag gtaagaaaca aacagcagca gaattatcga
     2221 gcgaagaaaa aaacaagatc agtcatcggg ctcaagcaat gaaaaaattg agtgccgagt
     2281 ggaaacaatg gctggaagga gagagataga tgcggtattt agtagtaagt gataatcatg
     2341 gagacagaga tatcctcgtt gacttagtag atcgttatcg ttctgaagtg gatatctttt
     2401 tccattgtgg tgattcggaa ttagaagctt ctgacccatt atgggataca ttccaagtgg
     2461 ttcaaggtaa ttgtgattat ggaccaggat tcgaacagaa aaaagtgatc caaactggtc
     2521 aggatacagt attcatgact cacggtcatt tatcgaatgt ccgatttggt ttgacacagt
     2581 tagcaatcga agctcgagct gcaactgccg atatggtgtt ttttggtcat acccatcaaa
     2641 taggctgtga gatggatcag aaaatcctgt ttttgaatcc tggtagcatt tctcagccta
     2701 gaggcccgat ccaaatccca tcatatgctc ttatcgaaag tttagatgac caaatttctg
     2761 ttcaatacta taaccgtgcc catcaggcag ttgaagagtt ggccttttct ttttcgagag
     2821 agcgctgaat aaaaaaactc taatttttaa taaaaataag ctttatttta tgaattttgc
     2881 atccggtttc ggtaaaatag tagtgttgaa tgaaatagaa atggaggcca tcagatgatt
     2941 ggacctatag taagagaaat gctgctggaa aatcaggaaa cgtttttgat tcctgcagaa
     3001 aatgtggcaa atgttatgta tcatcatcca ttgtcccatg gtttacttgt gttatcgaag
     3061 gtcggttatt ccaaaattcc tgtattagga aaagatgatc actttgtagg actagtaagt
     3121 ttatcagatg tggtcaataa aatgattgac ttgcaaacaa tcagcatgga cccgttagaa
     3181 ggactaacgg ttgctgatgt gacagaaaca gatgttccga caattgatga aaattgggaa
     3241 ctagaagaag tactccattt gttagtcgat tcgccttttt taccggttgt cacagaagat
     3301 cggattttca aaggaatcat tacaagaaaa gagctgctta aagccgtaaa cttcatggtg
     3361 catgaattag aacgtcgtaa tatattgtct ccgaaaatcg aagaattaaa agaacaaatc
     3421 gatatcgtga gctaaagaac gagaaactaa aagataatca aaaaataagt agaaaaaaca
     3481 gcgtaccagt tttgacttta tcttgaaaaa gaggctggga caaaagcgtt taactccaag
     3541 aaataagaag gaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc
     3601 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgggac aaaagtgttc
     3661 tttacttttg tcccacgctc tttttagtga gattcgttgc atcgtttgtc caaattattt
     3721 tccgacagca attggactgt tcggaatcgt gaaaccagaa gctgtcaatt ctttgacata
     3781 ggagctcaag aactcttctt ttaaggcata ttgttttccg ttcaatgcgt agcagaccgt
     3841 acgaatcgca aaattgctgt ttccaagatc cactaatcca aagatagaag gttcagtttg
     3901 gagctcttct tggtattttt cagctaatgt acgatttact cgatcaatga tctcatagat
     3961 tttgtcatat ccttcttcag gaacaatccg aatatctaaa agtacttgca tatttgcacg
     4021 agagagattg ctgatcgtgg tgatgtttcg attcgggatg aaatggacag taccatccgt
     4081 tgctttcagc tgaagggtac ggataccaac agatacgacg gttccttcaa tcgttaagtt
     4141 tgctagcttg atgtagtccc caacatccat ctgttgttcc atgataataa aaaatccggt
     4201 aataacatca ctcatgaacc cttgggcacc aagaccgatt gccacacctg caattccggc
     4261 accagccaaa agtgatccga tcggcacacc gatagcagat aaaattgcat agataaaaaa
     4321 gaaaccaagg gtataatgaa aaatagtatt taaaagacta tgtaaagtct ttaagcggct
     4381 ctcgtttaga atggcttttt tagaatactg attaaaagac ttatcaatca gatattttcc
     4441 cacacggtga agaatccaaa aaaggacgat caaaaaaatc agatataaac ttttttggat
     4501 aaaggcagcc aaaatggcgt cccaatcaat cccgtcccaa aaccgctgaa acgcatttac
     4561 tttgctgaca gcttgatctg tcaatgtctc gccagaagag tctgtcgtac cggcagttgc
     4621 taataatgaa aacataaaat cctcttttct aaaatttgat tctttctgta ttgtaccaag
     4681 aagttttcaa aaaaggaaat agcaaaaata actataatga aggaaaaaag ctgaaaataa
     4741 aatgacaaat aatatggata atgataaaat gtggagaaaa taacgaaggt ggtaagtata
     4801 tgcaaatcga ttggaacaat ctaggttttt cttatatcaa aactccttgg cgttttattg
     4861 ccaaatggaa agacggacaa tgggaagaag gagaattgac agaagataat tacctgacga
     4921 ttcatgaagg ctctccagcg cttcattatg gtcagcaatg ttttgaaggt ctaaaagctt
     4981 atcgtcgaaa agacggcaag gtcaatctct ttcgacctga acaaaatagt cgacggatga
     5041 atcaaagtgc tagacgattg ctgatgccaa tggtccctga aaagatgttc gttgatgctg
     5101 tcaagcaagt ggtaaaagca aatgaagatt ttgtgccgcc ctatggaaca ggcgctacat
     5161 tatacttgcg ccctttactg attggggtcg gtgaaaacat tggtgtccat ccagctccag
     5221 aatatttatt tgtcgtcttc tgtacgccag taggagcgta tttcaaaggt ggactaaaac
     5281 caacgaattt tattgtatct gattacgatc gtgctgctcc taaggggact ggtgcagcaa
     5341 aagtcggcgg aaattacgca gcgagtttac ttccaggaac agaagcaaaa gaactggatt
     5401 actcagattg tgtttatctt gatccagcca cacatacgaa aatcgaagaa gtaggtgcag
     5461 caaatttctt tggaattaca aaagacggaa aattcatcac tccgcaatcg ccctctatct
     5521 taccaagtat cacgaaatat tccttgttgc agttagctga agaacgacta ggactgcaag
     5581 caattgaagg agatattttc attgatgatc tagatcaatt tgcagaagct ggtgcatgtg
     5641 gtacagcggc tgttatttct ccaatcggtg ggatttatca caataacaat cttcatgtat
     5701 tttatagtga aacggaagta ggaccagtaa caaaacggtt gtatgatgaa ttgacaggta
     5761 ttcaatttgg agacatagaa gcaccagaag gctggattga agtcgtggaa taatttcaaa
     5821 gtaatgagtt gaaaaaaacg taaaaagaaa aaatcaggaa gagactttaa aaagtttttt
     5881 cctgattttt atatgtttta ctttaaagaa tgcaacaaaa gtttatgaaa gatggacacc
     5941 tttgtcaacg tagataatat caccgatgat tccgctggaa agtgggctga ttaagaaagc
     6001 acaagcatta ccgacttctt cgatcgtcac accttgctga tcaggcgtac gagattcaga
     6061 taactggatc agtttttgat agtctttaac acctgtgaca gctaatgttt tgattgcccc
     6121 agctgagatt ccattgaccc gaattccttt aggcgagaac tcagctgcca aataacggat
     6181 agcagcttca agagaagctt tcgcaatacc catcatgtta taattcggga ttgcgcgttc
     6241 tgaacctaga taagtcatgc taacgatccc gctgtttggt gcaagaaacg gttgtgcgta
     6301 ttttgataca gcaattaaag agtagctgct gatatcttga gctaattgat agccgtctct
     6361 ggaaatatca gacacatttc cggacaattc ttctttgttg gcgtaagcaa ctgcatggac
     6421 aaggccgtgg atctccccag catattcacc gatggcattc attgcttttt cgatgctttc
     6481 gtcgctagaa acatcacatt ctacagtgaa tgcttcttcg ccgaccaatt tgactaatga
     6541 ttttttcatt cgttcatttt ggtaagtata gataatttct gccccttgtt tcaacattgc
     6601 ttcagcacaa ccccaagcaa tgcttttttt gttagcgacg cccataatga cgattttttt
     6661 tccttctaga aatgacataa tgactctcct ttgtttctga taaaaatttg atagtcgtaa
     6721 aaaaatagag aataatagct aaaataactt cttgatttct taattattag tcaaaatgtt
     6781 ttgattgtca aactattttt tcttaaatta aaaaaagatt gctaaatatt taggaatatg
     6841 atactttgat agtcaaagta atgaaaatgg caattgcctg aaagaaggag tttaattatg
     6901 tcttacgtat tatctgctac tgaagtaatg gaattgattc ctaatcgtta tcctatttgt
     6961 tatattgatt atgtagatac tttagaacca ggaaaaaaga tcgttgcgac aaaaaatgta
     7021 acgatcaatg aagatttttt ccaaggtcac tttcctgaca atccagtgat gccgggtgcg
     7081 ttgattctag aaacactggc acaagcaggt tcgatactta tcctaaaatc agaagagttt
     7141 ctaggaaaaa gagcttatat cggtgggatc aataaggcca aattccgtca aaaagttgtt
     7201 ccaggagatg tgctgaaatg tcattttgaa atcaccaaaa tgaagggatc agtcggtaca
     7261 gcgttatctg cagcatatgt agatgataaa aaagtctgtg agtgcgagtt cacgttcatc
     7321 gtcaaccaaa atgcttgact ataaaacctg atatagcaaa acaaaaggaa gcttacttgc
     7381 tttttatgta aaaagaagac gcctgaatcc tagcatacgt tgtcatctat gcaggattca
     7441 gacgttattt ttttattttt gttttgcttc ttgcagtata ttgattttat cgatttttac
     7501 ttcttctttt ggtttgtctg aactatctgt ttcgatattt gcaatagcat ccacgacatc
     7561 cattccttca atcacttggc cgaagacagt ataatggcca tctagtgaag gataaccgcc
     7621 gtttttataa gcgtcgatga ttgcttgagg atagtcgtct tttaaaagac catcatgcat
     7681 atcatctgta ttttgaacga taaagaactg gctgccgtta ctgttaggat cttgtgagcg
     7741 agccatagat aacgcacctc ttatattata aaggtggttg ttgatctctg tttcaaatcc
     7801 ttctccccaa atgctttccc ctccagtgcc atcgccttta ggatcgccac cttggatcat
     7861 gaaatctttg atgacgcgat ggaaagtcag tccgttgtag taaccgtctt ttgcatgtgt
     7921 cataaagttt tcaaccgctt ttggtgcgtg ttcgggaaat aatttgattt tgattgtgcc
     7981 tttattcgtc accatttcga ccagatcttc attttcggcg acatctttcg tcaattgtgg
     8041 taattccaat gcgttcaaat cgacgctact ggcagtcgtg ctttccgcag tagatgtact
     8101 ttttgaactt tctgtagacg aactagaaga agtcgtgttg tttgcagaac aaccagcagc
     8161 aaacagcaat gctgctgcta aactaatact taaaataact ttatttttct tcataactgt
     8221 tcctttccaa tttactattt tgttatcttt aatgataaca aaatagaggg aaaataccta
     8281 cttaaattat tggaatttat catttatttt aaaagaaaaa gaaaaggtgt agaccattta
     8341 ctttgtgttt gctataatgt aagtggatac aaaaacgaaa agatgcagga ggaaatggta
     8401 atatgggaaa attaggaatc tcgatttatc cagaacggtc aacatttgaa aaggacaaag
     8461 cgtacttgga tttagctcat aagtatgggt ataaacgggt ttttaccagt ttgctgcaga
     8521 tcaatgatga taaagaaaaa gttctttcag aatttaaaga agtagttgat tacgcgaata
     8581 gcctaggcat ggaagtaatg gttgatatca atccagcgtt atttgaacag ttggagattt
     8641 cttacgatga cttatctttt ttccataaga tgggtgccta cggggtccgc ttagatatcg
     8701 gattcacagg agcagaagaa gcaaaaatga cgcgcaatcc ttttggtatc aaaatcgaga
     8761 tcaatatgag ttcgggcacg aattacgttg acaacatcat gagttattcg ccaaacacgg
     8821 ataatttatt aggttctcat aatttttatc ctcatcgata ctctggttta ggatacgaac
     8881 actttgtttt ttgttcagaa aaattcagaa aatataattt gaatacgatg gcattcgtca
     8941 attcgcaatc tgctgagttt ggcccttggc ctacgcaaga tggcttgtgc acgctggaag
     9001 accaccgaga tcttgaaatt gcgacacaag tgaagcattt aatccttaca ggattgattg
     9061 atgatatttc tattggaaat gcgtacgcaa gtgaagaaga attgaaagaa atggcagaag
     9121 catttaacgc ggattatcca acattgaaag tagatacgga agaaggtatt acagaaaacg
     9181 aacgaatctg cttgtttgat aaccttcata gttatcgagg ggatcgctca gaatatatcc
     9241 ttcgttcaac gatgactcgt gtatactaca aagataaaga tttcccgcct cataatacgc
     9301 gagatatgca tcatggggat gtactgattg acaatgaagg gtacggtcaa tacaaaggag
     9361 aaacacaaat cgccttgaag gatatgaaaa acgacggacg tgtcaatgtt gtaggaagaa
     9421 tctccgatga cgaattattc ctccttgact ttttgaaacc atggtcaagc tttaaattaa
     9481 ttgaaaataa aaaatagata attagttatt gcagaaataa tccgctagaa atagccagtt
     9541 atttctgctt ttttattttg tttttttata g
//
LOCUS       DASHFF010000090         9341 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.090, whole genome shotgun sequence.
ACCESSION   DASHFF010000090 DASHFF010000000
VERSION     DASHFF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.090"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1127)
                     /locus_tag="U2I75_002529"
     CDS             complement(<1..1127)
                     /locus_tag="U2I75_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1697591.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1156..1542)
                     /locus_tag="U2I75_002530"
     CDS             complement(1156..1542)
                     /locus_tag="U2I75_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1697592.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(1558..1872)
                     /locus_tag="U2I75_002531"
     CDS             complement(1558..1872)
                     /locus_tag="U2I75_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1697593.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(2225..2704)
                     /gene="rlmH"
                     /locus_tag="U2I75_002532"
     CDS             complement(2225..2704)
                     /gene="rlmH"
                     /locus_tag="U2I75_002532"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN1697594.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3132..4421
                     /locus_tag="U2I75_002533"
     CDS             3132..4421
                     /locus_tag="U2I75_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN1697595.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4460..5065)
                     /locus_tag="U2I75_002534"
     CDS             complement(4460..5065)
                     /locus_tag="U2I75_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN1697596.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(5261..5725)
                     /locus_tag="U2I75_002535"
     CDS             complement(5261..5725)
                     /locus_tag="U2I75_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1697597.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5747..6067)
                     /locus_tag="U2I75_002536"
     CDS             complement(5747..6067)
                     /locus_tag="U2I75_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1697598.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(6210..7289)
                     /gene="pepA"
                     /locus_tag="U2I75_002537"
     CDS             complement(6210..7289)
                     /gene="pepA"
                     /locus_tag="U2I75_002537"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN1697599.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7489..7839
                     /locus_tag="U2I75_002538"
     CDS             7489..7839
                     /locus_tag="U2I75_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1697600.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            8024..8977
                     /locus_tag="U2I75_002539"
     CDS             8024..8977
                     /locus_tag="U2I75_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1697601.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            8990..>9341
                     /locus_tag="U2I75_002540"
     CDS             8990..>9341
                     /locus_tag="U2I75_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697602.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSF"
BASE COUNT         2709 a         1831 c         1732 g         3069 t
ORIGIN      
        1 tgatcacgga ttccgtcgcc taaatatttt gcgtctggac gttgacaagc cagtattaga
       61 aagaagccag cttgacgacc taacatgaca atctgtttca gcttattcat aactgcggtg
      121 ttttcttttg ttcccagcat ttccatgaaa gcgacgtatt catcaaagat taagaagtgt
      181 gccgggagac ctaagtaagc ataatttttg ccagtcttat agttcttcat ctgcttcatt
      241 tcctcactac gtttcatcat ttcttcatag aatgtttcaa tgcaagaaag caagtcttct
      301 tttctatagt agacatttgc catcacagaa cctaagtccg caaggtcagc atttttcggg
      361 tcaagaatat acagttttga atctgtatga agcaaggctt caatcagtgt cagtataaag
      421 taagttttac cgccacctgt accaccagca atcaacatat gagggagctt atcatattcc
      481 caccatacgt ttttcattaa gcgaagttta ccatctttag cttctacttc atcaatagaa
      541 atacgactgg ctatggtgtc atagagcaaa gtatattcca cataggaatc ctttaactct
      601 ttatccgtca gctcacagta caagccactc tctaatttct tttccaagtg taagagttgg
      661 tcttgatatt ttcccagcgt gatttccacc cgtatctgta tcaagccatt tttaagtcga
      721 taatacattt tagggaagta ggttatcttt tcctttgtac gaccagcact atctttaaag
      781 aaaccctctg ttttgacctg ttcagattca taccacttgt tttcaagtat catctttgcc
      841 agtttttgac ggtggtaaag ttgtttaacc gtatcatagc gaacccgttt gaatacaaac
      901 gctaccagca agcagataag aattgcgaca ctgaaactga taattaaata gggaatgtca
      961 atcttatctg cttgtgatag gttaaaatcc tgccagttga tctgctggat tgtcttcaca
     1021 tgaaacagtc cgacaaccag caggaaaaca ggcaggagtg acgctatcgt aaaatgaaag
     1081 actaaatctt taccagatgg gcgaatcctt ttaccacgct gtttcatgcg aaaaagtctc
     1141 ctttctacct agcgactatt tgtcttgtgt cggttctttc tttgcttgtg gttgagcttt
     1201 gaatgaacta gaatcctttg tcagcacaat atcgtctgcc ttgatatacc agtcaacatc
     1261 tgctccttga taggtggcag tagcaacggt gtccgcaatg ggattgataa gttccacccg
     1321 tgcgttataa tcaaactctt tcaaaggcac gctggcagga atacttactt gaatcatgcg
     1381 tccttgtcct ttggatttta agtcataggt acgttccttg atttcatctg aaaccgaccc
     1441 gtcttcattt tggattctca cttcacgacg tagagcagag aatttcaatt ctccaaaagt
     1501 cgtgtcttta tctaatacaa tgccatttgc taatctcatc atttttcctc tctttcttta
     1561 ttcttttatc atgtcgtcag catgtaaaag gtaatttgta aaaccacgag tgccgatttt
     1621 gtagccctct gcggtaatac gtggattgac taacttcaca cgttcctcaa agccgaaatg
     1681 tttttcgcca gcttcagcag gaagcaccac cacaatatca tctgctcttt gaacatcaga
     1741 atagagatta tagcttcttg ataagacagt tagccgtccg ttgattcttc gctgaacgac
     1801 tttatcctcg ccagcaaatt ctaaattgcc gaatgttttt tccatgttgg gaatcacaaa
     1861 tttaagttcc atatttttac ctatcctttc ttttttattg gctgaatgaa tgtttgatgg
     1921 tcttaaagag tggggaacga ccttttgatt cttgattttt tgttttcata agttcacttc
     1981 ctttcaaaat cgggtaaaaa aatagacacc tcattttttg aagtgtctac ctattaaata
     2041 ttcaaatttt attggaagta tctttatatc ttcacttttc aaggataaat cgtcgtatca
     2101 aagctcattc ataagtagta aattagtagt aaattgagtg gttttgacct tgataaagtg
     2161 tgataagtcc agtttttatg cggataacta gatttttatg ctatttttct cttaaaaaat
     2221 atcattattt gtggtatgga gcccccgcgt tgatccggaa ggcacggtag atttgctcga
     2281 cgaggatcag gcgcatcaat tgatgtggat acgtcatttt gccaaaagag atctgagcat
     2341 tgcttctttt catgacggct tcgctgagtc ctaaagaacc gccgataaca aaaacaaggt
     2401 ggctttttcc ttggatacct aattggtcga tttgtttagc aaaagattcg cttgtgggat
     2461 ttttcccttc aattgctaag ccatagacgt attcttgttc tttgattttc gctaagatcc
     2521 gttctccttc tttttctttt acttgtctca tttgcgcttc gcttaaattc tccggggctt
     2581 tttcgtcagg aacttcgatc aattcgattt tcgcataggc attcagccgt ttgacatact
     2641 cattgatacc ttggatcaag tatttttctt ttaattttcc tactgaaata attttgatat
     2701 tcatgttttc tctcctttga tcttcacgct cactttgttt attataatca attttttcgt
     2761 ctatatgttt attttgacta tttttgttaa aaagatttcc ccaacagtta tgcacaacat
     2821 ccacaggttt atccacagaa aacagcgttg ttttacctta tttcatcaca ttcattaggc
     2881 aaacatacgt tttataagaa tttatccact ttatacacag gctgtggata tgttttatcc
     2941 cttatttttc caatcctatc cgcgtagttt tacacaatat ctgtggaaaa agaaaatcaa
     3001 atattttcat ctgaaaacaa tgatttttcc taaagttttt cataaaaagt tcaaagtctc
     3061 aaagtagact aaagtcaaca aataagacta tactaacagt agataaatga atgtaattgg
     3121 aggaggaaca catggataga aaaaatgtga caccaaagat gaaaaaaaat aaaaataata
     3181 gcctctggcg taagttgggt ctggggcttg ttggagggat cgttggagga ctactgacag
     3241 ctggcatttt ttatgcagcg atgggcactg gcaacaatag ttctactgct acaagcggca
     3301 atcaaaattc tgccggtgaa acagttgtgg aaaatgtcaa agtcaatgta gattctgata
     3361 tcaccaaagc ggtagataaa gttcaagacg ctgtcgtttc tgttatcaac ttacaaagtc
     3421 aaaaccaatc aagcggtttt ggcggattat tcgggcagca agaagaaagt agcagctcag
     3481 atgatagtaa tttggaagct tatagtgaag gaagcggcgt aatctacaaa aaagacggga
     3541 atactgctta tgtagtcaca aacaatcacg tagtagatgg acagcaaggt ttggaagtca
     3601 tgatgaaaga cggtacgaag gtaaaagctg aattagttgg aacagatgcc tattcagatt
     3661 tagcggtgtt gaagatcaat tcagacaaag tcgaaacggt tgcctctttt ggggactcaa
     3721 gtgctttgaa ggttggtgaa cctgcgattg cgattggttc cccattagga tctgaatatg
     3781 cgaattctgt cacttcagga atcatttctt ccttgaaccg acaagtaaca agtacgaatg
     3841 aatcgaacga aacagtcaat atcaatgcga tccaaacaga tgcagcgatc aacccgggaa
     3901 attctggtgg tcctttagtg aatatcgaag gacaagttat cggtatcaac tcaagtaaga
     3961 tcgcaagtac ttctgaatcg acttctaatg taagcgtaga aggaatggga tttgccatcc
     4021 caagtaacga tgtagtgaat atcatcaacc aactggaaaa agacggaaaa gtgacacgtc
     4081 cagctctagg tatcacaatg gtcgacttat ctgccgtttc tactcagcaa caagaacaaa
     4141 tcttgaagat tccttcatct gtaactaacg gtgtcatcgt gacaaatgtc caaacagcta
     4201 caccggcaga aaaagctggg ttgaaacaat acgacgtcat tactaagatc gatgacaaag
     4261 aagtttcttc tggtgtagaa cttcaatctg ctttgtataa gaaaaaagtg ggagattctg
     4321 tcaaaatcac ttactatgaa ggctcgaaag aaaaaacaac tactgttgaa ttgacgatcg
     4381 atcaatcggc attaaaacaa aatcaaaata gtgaaaatta ataaaaaaga ggttgtgaca
     4441 aagacttgtc acaacctctt tattttggaa atacttctcc gactacggca tcctcaggca
     4501 atactaagat tccttttttt tctggagcat ttggcaaatg caattcacga gcagaacaaa
     4561 tcattccata gctggctacc cctcgcaaag ttcctggcca gatcatcatt ccatctggca
     4621 tcatggctcc tggttttgca acgacaactt tttgtccagc ttcgatattt ggtgcaccgc
     4681 aaacaatttg caatactgtc ccattgtcca cttctgtctc agtaatcgat aaatggtcag
     4741 agtcaggatg cgggacgcat gtttttacat acccgacaac gatcttcggt tcatgatcag
     4801 cttcaagctg ttcttcgaat cctgcttctt taatcaatcg attcaatttc gctaattctt
     4861 catctgttaa gcttacttgg ccgttccctt caattgttag atgatcggag atatggaaaa
     4921 agttccaagc aacaacttga ccttgttcat ctttgatccg agcaacattc catttacgtt
     4981 cgacagtgtt ttctgttcct ttatcgtcgg ctatgataac catcaaagtg tctccgacgt
     5041 gtgctttgtt ataagcaaaa atcatgttct ttcctccatt atcctttgct gagatttatc
     5101 ttaacaaaaa attgcattca ctgcatgaga tttttttcat gctttatttc cgaagaagct
     5161 tcttctggaa caccatttat tcgtaacaag ggactgtgac aagacttttg tcacagtccc
     5221 tttactgttt gattaaaaag gttatacaag ttccacttgc ttaattactc ggctgttctt
     5281 cttcgtcaga aacgatcaag acatcacaag gtgaatgacg tatgacgtag ctggctacac
     5341 ttcctgtcat aaagcgttcg actgcattta gtccagactg cccaaccatg attagatcga
     5401 ttgcatattt ttttggcaat ttgactgcca tggattcacg aacagagccg tatgcaataa
     5461 ttccttctat atttgtgtaa tcaacagatt ttccatattc tttacattct tcgatcattt
     5521 gcttggctgt ttcagtttgc tgatcgacta gattttgatc aataggagca tagcccataa
     5581 aattataaaa ttgctgatcg attacgtttg ctacatatac tttcccatta ttacggcgag
     5641 ccacttctaa cgcttttttg aaagcagcaa gtgcttgttc actaccgtca atacctacta
     5701 aaatgttctt atattcttga ttcatcttga cccctcctaa cagatgtcag ccttttatta
     5761 cactttctag gaacgattcg atttcctgtt ttgttttacg atctttattc actagacgac
     5821 ctaattcttt tccgtcttgg atcacgacaa aacttgggat accaaaaatg ttccattcag
     5881 cggctacatc tatgtacttg tcacggtcta cttcaataaa ttgccaatca gaaaagtcat
     5941 tttcgatttc aggcatttgc ggtttgataa aacggcagtc tccgcaccag ccggcagtaa
     6001 agaaaaatac actttttcca gtttctacgt agcctgctaa ttcttctaaa ctatttggga
     6061 taatcattct ttttcctcta ctttctgttt atgtattttt caattaaatt atacaccttt
     6121 ctttcgaact aacaaacatt gatcctcttt ttataaaaaa aatctgacaa aaggttgctt
     6181 ttgccagatt ttatagtttc actatcgcgt tatttcccgt atacgatcgt gttcactgtt
     6241 gatttatcta aacctttcaa tgtttgaatc aacatttctc gtgcagcttc aaaatcgcga
     6301 atactgaaca tcgtttgatg cgtatggata taacgtccca ctacgccaat cactgtactt
     6361 gggattcctt cattttgtgt atgagcagca cctgcatctg taccgccttt tgaaacaaag
     6421 tattgataag ggatgttgtt tgtttcagcg atatctagca agtactcacg caaacgagga
     6481 agcatgatca aacctggatc ttggatacgc atcaatgtac cttctcctaa atgtccgaat
     6541 gtgccatttt tcgtcactgt gtcatctgct gcagagcagt ctactgcaaa gaacagatct
     6601 ggcttgaatt tattgacaga agctttagaa ccacgcaagc ctacttcttc ttggacattc
     6661 gcgcctgcaa tcaatgtatg acctaatgtt tcattttgta acgcttctaa agcttctaat
     6721 accattgtac agccatagcg gttgtcccac gatttgctga tgatattctt cccgttggct
     6781 gttttgatcg tttcagcgta aggaacgatt gtgtctcctg gaagaacacc gtaactcatt
     6841 gcctcttctc ttgattcaaa cccagcatcg aacaaaatat ctgttacttc tactgatttt
     6901 tgtccgcttg ttcctcgtaa taaatgtggg ggaacggaag aagaaataca tggataattc
     6961 ttaccagtag aggttttaag tgtaaaacgt tgagcagaaa caacgtaagg gttccatccg
     7021 cctagaggta cgactttaaa aagcccgttt tcttggattt gtgtcagcat gaatccgact
     7081 tcatccatat gtgctgcgac cattacacga ggtgcatccg cttcttcatg gtggcgcagc
     7141 ccaaaaatgc cgcccaaacc atctaactgt aactcatcta ccagcggttc catatgactt
     7201 tccatatagg cgcgaacgtc ttgttcaaaa ccgcttgttc cttgtaattc tgttaactct
     7261 ttgattcgtt gaaatgtttt ttcttccata tgctattcgt ctaaatttca gactaaactc
     7321 ctctcagact tatattgatc tgcggataaa atatgatttt ttctgcagac cgatcgtatt
     7381 tattatagct tattttttct ggtagcggtt atttatttca aagaaaatat gagagtgtgg
     7441 taaaataaac acaaagagta gcaagtgaga agaaggaggt atatccacat gtatgaagaa
     7501 agcgaattaa gctattttaa aggcgggtta gccgttggga tcggtctagg attattgagt
     7561 ggggtagctt caacgatttg gtatcatcgc aaaaaagtgt tatcagcaga ccaagtgctt
     7621 gaaaatatca aaacatcttt tttaaaagaa ggcgcaatcg aaggtgcttg gatctctttt
     7681 gaaaaagaac ctattcggaa attcgccgtt cggtcgagtg cttatcgggg tgggctttcc
     7741 cggatagaag atggcgaaac tgtttattat gaatttttag cagatgctca tacaggaacc
     7801 gtattagaca tttcaagaaa aaagggcgca atagattaat acgtcaaaaa aatgaaataa
     7861 cgaatacgca cccactatct aaaagagagg tatcctatcc ttcttttata gtgggtgatt
     7921 tttataaaaa tagggctatt tcctcttgga atagctagta tttgtagtta ttatacggta
     7981 ttcttgaaat aagattagtt ttattatata aggagagtga caaatggaat cacgtgaaaa
     8041 aattgccgtg ctcattcctt gttataacga agaggcgaca atttcaactg ttattgaaga
     8101 ttttaaaagg gaattaccag aagcagatat ttatgtctat gataataatt cgaccgatcg
     8161 aacatacgaa ttggccgttc aaggcggagc aatcgtaaag aaagaaccac gtcaaggaaa
     8221 agggaatgta atccgtcaaa tgttttttga tattgatgcc gattattatt tgatggtaga
     8281 tggtgatgat acttatcctg cagaagctgc acatggtttg cttgagaaat tacgttctgg
     8341 cgaagcagat atggtcattg gcgatcgtct atcaaatgga acctattttg atgaaaataa
     8401 gcgtcctttc catgattttg ggaataatct ggttaaaaat acaattactt acttatataa
     8461 aacaaaaatt cgcgatgtca tgaccggcta tcgaggattc aatcgaattt ttgtcaaaag
     8521 cttccctatt atgagttctg gttttcaaat cgaaacagaa ttgacgattc atgctttaga
     8581 taaaaaattc aagttcgttg agattccaat cgattatcgc gatcgtcctg aaggtagcga
     8641 atcaaaattg aatacattct ctgacggatt caaagtgatc atgatgatca tcaaaatgtg
     8701 caaagattat aaacctttgc ttttctttgg gatctggacc atcttcttct ttttatttgg
     8761 attggtcatg gggattcctg tgattcgtga atatatgctg acaagcttta ttacacgcat
     8821 cccttctgcg attttaagta ctggattgat gactttagcc cttctttcat tagtaacagg
     8881 gttgattttg gatacggtcg tcacaaatgc caagaaagaa tatgaattga atttatacca
     8941 tgtctaccac gaatatcaaa ataaagaaat gaagtaggag gcctttttta tgcataagaa
     9001 aaaggagaat ctatttccta ctttttttgc aactttattt gcgtcaggtt ttgctttttt
     9061 tacttcttat ggtctaggaa ctttgaatcc aacagtaatc attggtttag ccatggtttt
     9121 gctacttgct tttaagcaat atggaataga gttgttttca ataaaaaaca aagttgcagc
     9181 tattgcccga ttgttttttt ctgcagcttt tgctcttaca ctttctattg gcagacagat
     9241 acaatttcag tatgtctcag caaactatac aaagacaacg attgctgatg gaagtatttt
     9301 ttactttctt gtttatttct ccatttttgt gtattctttt t
//
LOCUS       DASHFF010000091         9242 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.091, whole genome shotgun sequence.
ACCESSION   DASHFF010000091 DASHFF010000000
VERSION     DASHFF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.091"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..537
                     /locus_tag="U2I75_002541"
     CDS             <1..537
                     /locus_tag="U2I75_002541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697603.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..2375
                     /locus_tag="U2I75_002542"
     CDS             564..2375
                     /locus_tag="U2I75_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697604.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAENKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2436..3896
                     /locus_tag="U2I75_002543"
     CDS             2436..3896
                     /locus_tag="U2I75_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN1697605.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3907..5916
                     /locus_tag="U2I75_002544"
     CDS             3907..5916
                     /locus_tag="U2I75_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEN1697606.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6032..6181
                     /locus_tag="U2I75_002545"
     CDS             6032..6181
                     /locus_tag="U2I75_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697607.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6262..6858
                     /locus_tag="U2I75_002546"
     CDS             6262..6858
                     /locus_tag="U2I75_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN1697608.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6871..7770
                     /locus_tag="U2I75_002547"
     CDS             6871..7770
                     /locus_tag="U2I75_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN1697609.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7773..7904
                     /gene="ssb"
                     /locus_tag="U2I75_002548"
     CDS             7773..7904
                     /gene="ssb"
                     /locus_tag="U2I75_002548"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1697610.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7926..8258
                     /gene="ssb"
                     /locus_tag="U2I75_002549"
     CDS             7926..8258
                     /gene="ssb"
                     /locus_tag="U2I75_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1697611.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8280..8537
                     /locus_tag="U2I75_002550"
     CDS             8280..8537
                     /locus_tag="U2I75_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697612.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8696..8818)
                     /locus_tag="U2I75_002551"
     CDS             complement(8696..8818)
                     /locus_tag="U2I75_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEN1697613.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9008..9232
                     /locus_tag="U2I75_002552"
     CDS             9008..9232
                     /locus_tag="U2I75_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697614.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
BASE COUNT         3435 a         1565 c         1966 g         2276 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tctcctgaag
     1141 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
     1201 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
     1261 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
     1321 tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga aatagaagct acaccagaag
     1381 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaaacttac tacacgattg
     1441 aaaaagtgtt tgatatcaca caaacacagt taaaacctga agactatccg aaaatctttc
     1501 cgaaccgtgt ctttgatttt caattggata aaaaaggaca gtcagaactt caagctggga
     1561 ttcaagcggt agccaacaat attggaatac aaattcgaga tatgacagaa agtgaagtct
     1621 atcaaagaga attaggacaa gcacgtgggg cgtatgttcg taacgaattt actggcgaag
     1681 aagaaatcgt catgaatact cgaaatactc ctacgcagca tttagctaca agtattcatg
     1741 aattagccca tgcaagaatg cacaaatttt cagaattaga taccgcaact aaagaattgc
     1801 aggcagaaat gacttcttat attgtgtgca aacattttgg aatggacacg ttggagaaag
     1861 ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat aaggaagcgg
     1921 cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc attcaaacca
     1981 tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca gcagaagtga
     2041 agcaggagca taaaaatact gtagaaacgg cagagaataa gaatattgtg cccgttggtt
     2101 cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt cgaacgttac
     2161 atttgaagga tgcggcgttt attgaagatc cgcagcataa tcaagtgatt ttaaaaggaa
     2221 aaagttatgg aaaagagatt caaacggcgt taccttcaga agaatttgcg ctaggaaatc
     2281 cattccgatt ttcacttgaa acagtgtcag aagtggccaa acgaaaaaat gaaatctatt
     2341 atgaacaaca attgtcacaa aaagaacgcg tataaaacaa gtaaaaaaga ctatctgaaa
     2401 aacggatagt cttttttctt ttgggaggtt tgaaaatgac cgaaaaaaaa atgagtctta
     2461 ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga atggctgtag
     2521 tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt gatcgaagaa
     2581 aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa ttagctaaac
     2641 tttttttcat agacaaagag atccaagact ctaagcaaca atttaaagca gccactgatt
     2701 ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt gaaacagaaa
     2761 agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg aattatctga
     2821 aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa gggttgattg
     2881 ccgaactaaa gccaaaatat gaacggcaaa atttcttagt tcgagacgat cgtttagatc
     2941 atgcggtcgc ctttttatgg aaagatccac agacaaaaga gacagtaggg gcgagttatc
     3001 aaggaacgtt cattgattat gaacgttttg gtgagagagg cacatataag catatagata
     3061 aaaattcgac agcgaatcat ggctttaacc taaagatcgg tgaccctaag cagttaaaat
     3121 tttttgagag tagtattgat ttacttagct atgctgcact gaatcgtgat cagttaaacg
     3181 atacgtggtt agtctctatg gaagggttaa aacaccatgt gatcagtcat tattttggtg
     3241 aggcagtatc tgaattgaga aagaaacaag cctttcctca atccatcgag atttgtgtgg
     3301 ataatgaccg tgcaggacat attttctacg aaaaagaaca attaatgggc gcagtcgatc
     3361 catttacgaa tcagaaagta cgttgtgaac gaggaatcgc taatgattgg caagtgccaa
     3421 aagaatacaa aatcatttat gaagaagtcg caaaagaaga aaaggtcaca ccagaagcta
     3481 ttatggccat acacaagaca gaaaacaatc tgcaattaac gaaccaactg gtttccgctc
     3541 acaaagtaaa tgcgtttttt ggccagcagc tttctgttaa cgattcaata gaagccatta
     3601 atttgaaaga tatttgtaga gaagtcgcaa aagaattaaa agtttgtgaa cgcgtggatg
     3661 gtacgtacga ttttgatcgt ttttatcaag aaaaagggga tattaatgcg cagattcttt
     3721 tctcttataa agcagaacaa tactacaaag gctataaaaa tcatgaacat gaatttatac
     3781 ctgaagtaaa aaaggattgg aatgatcaat tgaagcatga gatccagcaa caagaaatca
     3841 gaaagcaaaa acgtgccatg ttgtttcaac aaggcagaca acaagaaagg gagtgaaatc
     3901 gtcatgatga aaaccgttat tttagcagaa aagccgtcac aagcgaaagc ttatgccgat
     3961 agcttctcta aagccactcg aaaagacggg tattttgaaa tccaagatcg attgtttcct
     4021 ggtgaaactg tgatcacgta tggttttggt catttagtcg aattagattc gcccgatatg
     4081 tacgatgaaa attggaaaca atggtcatta gaacatttgc ctattttccc tacacattat
     4141 cactatcatg ttcctaaaga taagaaaaag caattcaagg tggtgaaaca gcaacttcaa
     4201 tcggccgaca ccattattat tgcgacagat agcgatcgtg aaggggaact gatcgcttgg
     4261 acgatcattc aacaagtagg cgccgatcaa ggaaaaacct ttaaaagact ttggatcaac
     4321 tccttagaaa aagaagccat ttatcaggga ttccagcagt tacgagacgc aaaagagacc
     4381 tatccaaaat ttgaagaagc acaagcacgt cagatcgccg attggttaat tgggatgaac
     4441 ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag cttttcctta
     4501 ggcagagtcc aaaccccaac gctttacatg atttatcaat tgcaggaaaa gattagaaat
     4561 ttcaagaaag aaccttattt tgaaggaaaa tccgaagtaa tcgctcaaaa tggcgcattt
     4621 gatgcgaagc ttgatccgaa tgaaacccaa gcgacgcaag aagcatttga agattattta
     4681 aaagaaaagg gggttcaatt gggaaaacag ccaggcatga ttcgccaagt tgaaaccgag
     4741 aaaaaaagcg cggcaagtcc acgtcttttt tctctatcca gccttcagtc taaaatgaat
     4801 cagctcatga aagctagtgc caaagacact ttggaagcca tgcaaggctt gtatgaaggg
     4861 aaatatctaa gctatccacg aacagacacc ccttatatta cggaagggga gtatgcgtat
     4921 ttgttggatc atttagatga atacaagcat tttttaaagg ccgaatcaat cccaacccct
     4981 attcatacgc cgaatagtcg gtatgtgaat aacaagaaag tccaggaaca ttacgcgatc
     5041 attcctacaa aaacggttat gacagcagcg gcttttgaac aactaagtcc actgcagcag
     5101 gctatttatg aacaagtgtt aaaaacaacg gtggccatgt ttgcggaaaa gtatacctat
     5161 gaagaaacaa cgatcttgac gcaagttcag cagcttcagt taaaggcaat cgaaaaggtg
     5221 ccgctagatc taggttggaa gaagttattt ggaaaagaga gcgacggaaa agagaaggaa
     5281 gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg tgacggtgga tctacaagtt
     5341 cttgaaaaag aaaccaagcc accacaacct tataccgaag gtacgttgat cactgcaatg
     5401 aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat ccatattgaa agaagtggaa
     5461 ggaatcggta cagaagcgac acgggcgaat attattgaaa ccttgaaaca aaaggaatat
     5521 atcaaggtag agaaaaataa gctcgtggtg acgaataaag ggattttgct ttgccaagcc
     5581 gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg ccaaatggga aagctacttg
     5641 ttaaagatcg gtgaacgaaa aggaacacaa accacttttc tagccaatat ccaaaaattt
     5701 gtctctcact tattggaagt cgtaccaggg caaatccaat ccactgattt tggatcgacg
     5761 ttacaagaag tgaaagcagc cagtgaaaag caagaagccg cgcgtcattt aggaatttgt
     5821 ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg tggcagcttg tacgtctgaa
     5881 gcctgtgatt ttaaaaggtc agaagggttc atttgaagca acgattgttt taaaagaaga
     5941 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
     6001 ccgcactgca aaataatgga gggttaatct tatgcgtaat accacacaca aacaacaaga
     6061 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
     6121 aagactgcag caattgaaga accagcagag gattcaaaaa gaaatcgaat tagatcgata
     6181 aaatcttcct aactattgta tacgtataaa cttatgttat actgttttta taagtttata
     6241 cgtatacaaa ggagggagaa aatgacaaaa gcaatggtat acaagctgct agaagaattt
     6301 aatggaactt tggctttgaa ggatgccaaa aaagcgggca tttctcctgt aacgatcaaa
     6361 cgtatggtag atcgagggga gctggatcga gagtatccag gttttttcac cttacctggt
     6421 caatttcctg atgaattgtt tatggcgcaa aaaaaatatg aacgcggcat tatttcacat
     6481 attaccgcgt tagatttgta tgatttaaca gatatgatcc ctagacagat cgatctgact
     6541 gtaccttacg gttatcacgt ttctgaaaaa ggattgaagg aattcgcagt tgagctacac
     6601 tatacaaaag cggaatggta tgaattaggt aagattgaaa taaaaagtcg ctatgggaat
     6661 cctattatag cttatgatcc agaaagaact ttatgtgaca tttggaatcc ctggtatacc
     6721 gttgaggatg aaatcaaagt aaaagcaatc aaaaactata tggagtcgga cagaaaaaat
     6781 cttagaaaat tgaatgagta tcgacgaatt ttaccaacag acaaaacgat gcgttcttat
     6841 attatggctt taaattaaag gagaagatac atgacaccta cgcagttaaa agcaaaagtg
     6901 aagaatattt ctagagaaaa agaagttgat ccgcagcttg ttttaagaca ttttatgatg
     6961 gaaaaattct tggaaaaaat ctcagagtct ccttatcgtg aaaattttgt gttaaaaggc
     7021 ggcttcttga ttggctcaaa gtatggcatt gaaaatcgca caaccaaaga tattgatacg
     7081 actttacggg aaatgaaagt aaccaaagaa acattaacaa cggttctaaa cgaaattttt
     7141 tcaacgccga cgaaagaagg cattcaattt gagattcaag gaatgaaaga aacgagagaa
     7201 gccgattact atccaggatt tagcttacgt gtgctggcac atttggaaaa catgcggcca
     7261 gattttaagg tagatgtgac tacaggtgac tcgatctatc ctgcaacgat cactcacagt
     7321 cataaattga tgtttgagga tcgaacgatt cctttggaat cctatcctac agaacaaatt
     7381 attgctgaaa aattaagtgc aacctttgat tttttaacag ataattcgag aggaaaagat
     7441 ttttatgatc tctatactat tcctaaaatg gagaagatcg atgagaagac cttgaaagat
     7501 agtgtgagaa atacatttac acgtcgagga aaaactgatc cgttaaagat atactatgaa
     7561 aaggatatgg atgtactgaa aaatagtcac gagctaaaag atacctggaa gaaatatcaa
     7621 aaaagcaatc cttatgctgc gtcgatcacg tacgatgaga caatggattc gattagtaat
     7681 ttgatggaaa aagtgattca cgtcgaagaa aaagagcgaa aactagctta tcaaagacac
     7741 caacagatga atagagaaat ggagagataa agatgattaa taatgtgaca ttagtaggac
     7801 gcttaaccaa agatcctgat ttacgttata ccgcaagtgg tactgcagta gcgactttta
     7861 ccctagccgt caaccgaaat ttcacgaatc aaaacggcaa ttgagaagcc gattttatta
     7921 actgcgtgat ttggcgcaaa ccagcagaaa caatggcgac attagctaaa aaaggtagct
     7981 tgattggtgt agttgggcgg attcaaacgc ggacttacga caaccagcaa ggacaacgtg
     8041 tatatgtgac tgaagttgta gcagataact ttcaactatt agaaagtaaa gcagcgacag
     8101 agagccgtgc gcacgctgat caatcgtcca ccagtccaag caccgccacc tttgaacaac
     8161 ggaatacagc gacgcctaac aacaatggat tgaacgcttc acagaatcca tttggtggac
     8221 aatcaatcga tattagcgat gatgatctac cgttttagtt tgagaaagaa ggagtaagca
     8281 tggcaaaaaa aattagtctg acgatggatc tattgatacc cgtaattgca tttatagacg
     8341 ggatttcttt gggcgtcact gaaaatcctt tggtaaatag aattgcgaaa ggatcactat
     8401 ttcttgtatt cgtgtatctt gtctgttctt atcttttaaa aagagcagga aaggataatt
     8461 ccttttggcg attgactaaa gtcacagtag cagcgacagc tgttatactt acggctattc
     8521 taccgcttta tatgtagagt taacgggaaa tcgttgaact tcttgcgaaa tattgctata
     8581 atgaacatga aaaagaggag ttgcgctaac aacccctcat taacagcacc gattaaagaa
     8641 cggtggcaga atattagtta tttggaaata accgctgacc tgctaaagta gacggttatt
     8701 tctttttgtc atttttaagc atatttacaa tcaaactaat caaagcaatg gtaaacatac
     8761 caaaaccaag aatggtttga attgtctcat atgcggacaa aaggcttact cctttcatag
     8821 attttagcac ttacattcat aagcaccacc acctttccag aagtcgccac cgtcttcaaa
     8881 ctttactgtc ttgactatta tagcatatct aaaatcgaat aaaaattctc aattttagct
     8941 gtttttgcta taattttagc gaggtgatcg tgatggaaca atatattggt ttactcgata
     9001 aattagggtg atcaagacac agccattgct tgtgcgattt acattacgca cgagtcatga
     9061 accaatcaat tgtgtagtgg ccaatataga aatagcggat aaattgctga ttttggaaga
     9121 aggcaagtat actatttcag tgacaggtca cttcaataaa cgaaatcaac tagtcattga
     9181 atcaatgcaa attagaaacc ctgatcattt tacgagatta atgggcatat aaataaaaga
     9241 ag
//
LOCUS       DASHFF010000092         8937 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.092, whole genome shotgun sequence.
ACCESSION   DASHFF010000092 DASHFF010000000
VERSION     DASHFF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.092"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(25..318)
                     /locus_tag="U2I75_002553"
                     /pseudo
     CDS             complement(25..318)
                     /locus_tag="U2I75_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
     gene            complement(346..1011)
                     /locus_tag="U2I75_002554"
     CDS             complement(346..1011)
                     /locus_tag="U2I75_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN1697615.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1151..1783)
                     /locus_tag="U2I75_002555"
     CDS             complement(1151..1783)
                     /locus_tag="U2I75_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1697616.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1790..2857)
                     /locus_tag="U2I75_002556"
     CDS             complement(1790..2857)
                     /locus_tag="U2I75_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1697617.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2854..3585)
                     /locus_tag="U2I75_002557"
     CDS             complement(2854..3585)
                     /locus_tag="U2I75_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN1697618.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3754..4233)
                     /gene="greA"
                     /locus_tag="U2I75_002558"
     CDS             complement(3754..4233)
                     /gene="greA"
                     /locus_tag="U2I75_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN1697619.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="U2I75_002559"
     CDS             complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="U2I75_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN1697620.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5748..6917
                     /locus_tag="U2I75_002560"
     CDS             5748..6917
                     /locus_tag="U2I75_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1697621.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7184..8818)
                     /locus_tag="U2I75_002561"
     CDS             complement(7184..8818)
                     /locus_tag="U2I75_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697622.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2840 a         1698 c         1326 g         3073 t
ORIGIN      
        1 aaaattgtgt caccctctct ttttttattg ttttttaaag tgataggttg gctcctccag
       61 gttgggatca taatcaacaa gtaaatcata tcctttaaag aaccattcat ctgcttcctc
      121 gataaaaaaa agtgtcccat caatttccac tttctttaca ggatgttctg gctgttcaac
      181 tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt ttcaaaaaaa
      241 acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt gtgcttttgg
      301 cgttaccgtt aatttcatcc caacaaccta ctttctcttt tctttctatt gattcatctt
      361 atcgtctaga atatccacat cgcctgtctc tccaataacc agtagattat cattttcacg
      421 aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc gtatggcgac
      481 tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt tgaaaaattt
      541 aggattggag actcggattt ctgctaaaga atatttatct gacaactcta ggtaatctaa
      601 aatattcttt gacactaaat tatgcgcaat ccttattccc atgtctcgct cgggatggac
      661 gacgcgatca gctccgatct tttcaagtac tctggcatga tactcatttt gggcttttgc
      721 taaaatattt ggtacgccca tttcctttac catcagagta actaagatac tggcttgaat
      781 atcttctcca atagcgacaa ttacatgatc gaaatttctt atgcctaatg atcgaagtgt
      841 catttcatcc tgtgcatttg ccacaacagc atgagtggct atattcatat attcattgac
      901 ccgatcttca ttactatcaa tagccagtac ttcttgacca gcttcgatca gtgttcggca
      961 gatacttccg ccaaatcgtc caagacctac aattgcaaaa ttttgtttca ccgttttcct
     1021 cctttagtat cttcttttct cagtataaca aagcctttac aaaaacaaaa gcaagcatca
     1081 ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat tatcctcttt
     1141 atcatcgttt ttatttcacc aaaccatgtt tgaatgcata gatagcggct tgtgtacggt
     1201 cttccacttc tagcttagcc aatatattgg aaacatgtgt ttttactgtt ttcagtgtaa
     1261 taaacaattc gtccgctatt tcctgattac tttttccttc agaaatcagc attaaaattt
     1321 cattttcccg attcgttagt tcttcatgta atacaggctc atttcgccga ctcatttttt
     1381 ccatcatttt tgttgtcact tctggttcta atactcgctc gccacgttgc gttgccctta
     1441 tagcatcagc aatttcatgt gcggtagagg tcttcaacaa ataccctgct gcacctgctt
     1501 caatggcagg ataaactttt tcgtcatcaa taaaactggt tacgataatg attttagctt
     1561 ctggccaatc ttttaagatt gcttttgttg attcgatacc atccatttct tccatgacta
     1621 agtccattaa aatgacgtcc ggtcgaagtg tcatcgcctt ctcatagcct tgtcgtccat
     1681 tttcagcttc tccaattact tcaatgtctt cttgaataga taaataagaa gagacgccta
     1741 agcgcaccat ttcatggtca tctactaata aaacttttat catccttatc tatgcctcct
     1801 tcatcaaagg gaccttgatt tctacacttg taccttgacc tttaaaacta atgattttta
     1861 ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa cttcccgttt
     1921 tcgtttcatt catattaaac cctgttccgt cgtcaataat acgtaacaaa agattattgt
     1981 ctattttgtg caaatatacc tctaattcat tggctttagc atgtcttaat gtatttgata
     2041 acaattcttg aacgattcgg aacaagtgat cctcaatgga actagatagt tttacatctt
     2101 ctacatccca tttcagcgaa atctggattt tattttgcaa ttccttcaat aactgctcga
     2161 taccttgctt taaacttttc tcttctaaat tgactggacg tagatgaaga agcagcgcac
     2221 gcatctcaga ctgggaagcg ttgatgatct ctgctaccat tttcaactgc ttgcgaaaca
     2281 tttctggtgt ctcgcttttt tctgcttgct ccgtcaatgc cgacatcatc atcattgctg
     2341 caaacaattg ttgcgaaact gaatcatgca gctcacgggc taatcgatgt ctttctaatt
     2401 ctaaaatttc ttctttggtt tgtccatcta catattgggg acggctcgtt acaatttgta
     2461 attcactaga tacttcaatc attttttctt ttatcttagt gatttcctta tcaatatctt
     2521 tgatgtataa atcgtccgat gcacttggga tcggaagaag caacaatttg gattcataat
     2581 tgccagaaga aagtgcacgc agcttttctt cgattttccc gtattttgtc ttttgaataa
     2641 gtgataataa tagaaagaca attaatccta cacctaagga aatggctaac aaatggaaga
     2701 taagcggaac atataataaa cgtgcacgca gtaattctcc ccaccaatga ctttgattgc
     2761 tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca atccccgaat
     2821 aaacagctag catcgctctg gatatttttc ccatcatatt cgtatcacct cgacatctcc
     2881 aatcaaggta ttagtaataa tcttcaatct acgagggttt tcatcataat cactgctgta
     2941 gattttgatt tgttcatttt tcaaagaaaa ttgctcttct tcaaacaaga catttcctac
     3001 taatgtcgca tgctctaatc ggatggctac gcccaaaggt actaagatcc gtgttctgcc
     3061 tatccctttt cttacaatca caatattatc atcttttggc aaaagcgtat tgcccaaatc
     3121 tatgatagta tcaccagaaa tgacggcgat gttgatatcg tcccattcat agacgtcgtt
     3181 tccaatacgt tggttaccga ataattgctg tcttttccgc tcattattat gtgtttttaa
     3241 ttgctctgtt tggatcatca tgatctgttt ctttcgccaa aaagcatttt tggttaaatc
     3301 tatccctgaa atctctacac ctttcagacc aataaataaa acggcaaata cagccatcat
     3361 ccataaagca ggactgttga ctaagctaaa aaaaatgacc aaacttccta atatcaattg
     3421 aaaattttga aatttagtac gaggatgttt gcgcatagct agatatatat tgaaaatacc
     3481 gaatatcact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag caaataacag
     3541 cagtagtgct tcaactacca caaaaaaacg ccaagaacta ttcatcgctc tttctccctc
     3601 ctgacacatt ctcatttcat catacctatt tttttaactg ttggctatag gactttagat
     3661 gtaagatcgt ttgttgaaat ctaaaataca caaagagaaa aagatctaga gtgtatcatc
     3721 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
     3781 aactaacata tcgccacctg gagtagcaat agttacttca tcatttttac gtttgccaat
     3841 caatgcttgc gcaataggag aatcattgga aattttgcca gaaaacggat ctgcttcagc
     3901 gcttccgaca atcgtgtact cttcttcttc tccatctggt agttcgatga atgtaactgt
     3961 tttaccaatt gatacctcat ctgcatctac accgtcgtta tcgatgattt gagcaaaacg
     4021 aatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttcgctga
     4081 ttcatattca gagttttcgg aaagatctcc gaaaccgcgg gcaattttga ttcgttcaat
     4141 gatttctccg cgtttgactg tttttagttc ttctaattct ttttctaatt tttctttacc
     4201 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
     4261 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttcttc
     4321 aaatgtaaat gtttttctaa aaatgatcaa tgtctagagc ctgtttttct aagaattatt
     4381 tacatatttt tccactaatt ccatatgttc ttcataagtt ttagcgaaat aaacatttcc
     4441 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
     4501 gattgcctct tcgcttggat tgtcaaaagg tccaggtcca tagccggtat tggtatacaa
     4561 attatatggt gaatctacag ctgtatcttc atacgtcact aattctttgt gttcacctaa
     4621 tgcgtataaa acagagatat ctgactgaag tggcatattt gctttgattc ggttaaagaa
     4681 gacttgtgca atgtttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
     4741 tagagttaaa acttcttgta cagtcaaatt tttctgcttg atagctgaat aatagttttg
     4801 catgaccgta ttcgtcttgt tcaccatttc aatcactaga tccttaagcg aagtactcgt
     4861 gtaataatca tatgtggctg ggaataagta accttccaaa cggtattttg tgttctcagc
     4921 tttagatgca ctttccaaca gttctgggaa tttttggtgc aactcgttaa agaaagtctc
     4981 atcattcact aaatctaaaa attctttctt gtctttacca gttaccttcg ctacccgttc
     5041 agcaatctga tcgatatcgt acccttctgg aatggcaatt ttcccatctg caacttttgt
     5101 tggctcagaa gtaccgcctt cttgcaactg tttgccaatc tcgtcaagag tcatattagg
     5161 cgctaactga taatagccag cttgaaagcc agttaaattt ttaaattttg tataataatt
     5221 gaaaacaatc ccgcttttaa taatattgtc cttctccaaa atctcaccaa tctgcttgtt
     5281 agaagagcca ctcgggatct cgacttggac taattggtca tctgatttat ctaaaggctt
     5341 caacccgcta tctacataac gatacacggt aaatccaaga aatccgccaa taatcaataa
     5401 tgtaagagca atcactaaaa tgatcttcct cacaatctta tcttcttttt tacgcagatt
     5461 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tatctttact
     5521 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcaagttc gtaactttat
     5581 tcattataca tgatatttga attggcgtga atgattttat ttaaatattc tatcaaaaat
     5641 gtaagtagta aatccattac tataagatta aaataaatgt tatcaagata caagctattt
     5701 tagctttatt ctttctgtaa aaaagaaagg agactaaata aactattatg aaaaaaggaa
     5761 aacatgctaa aaaaagaaaa tattcatttt taatgatcgt tgtcgtgttg tttagcctta
     5821 gtgcgttctc tacaggaact attcttggat ggcaatttta caataatgaa cactctcaga
     5881 tagttgccca tgctcaatca gctcaaaaaa agttgatact gactaccaat gaacaacagc
     5941 tcgttgaaaa aatcgatcaa ctattaagaa aaaatgatta tattggctca atttacgtaa
     6001 agaaaaataa tcgcatcatc atggaaaaag gctatggcta tgcgaataaa ctcacgcaac
     6061 aaccaaatga tccttctctt tattatcaga ttggttcggt tcaaaaagca ttgactactc
     6121 ttttgatttt aaaacaagtc gagcgtgggc acctttcttt agatactaag ctggctcaat
     6181 tctatccaca aattgctggc agccagcaaa ttactatccg tgacttgctg tatatgcgct
     6241 caggcttgca ccgaactgct tctccaacca taccgatgac tgatgaagaa gtcgttcaat
     6301 ttgcgattca gcacttaaaa tttacagatt atcagacctc tcattatgat tcattgaact
     6361 ttaccatatt gactggaatt ttgatcaaat taacaaatca accgtatgaa aaattattga
     6421 ccgatgatat catcgaacct ttaggattac agcatacaaa attttatgac caagtcaaaa
     6481 attcatccaa tcatgcacta tcctatcaaa tgtctaaaga ggatgattac tttaaaccac
     6541 tgacagaatc tgaaacagag atccataacg aattagggac tggtaatatc agtatgtccg
     6601 tctatgattt aaatacgctg tttacacaag ttttatctgg aaaactagtt tccaaagact
     6661 tactcttctc attatggcgt gaaaatgcga atggacatcc atatagtggt ggggtatata
     6721 gcggagataa ttatatcctg tcgcaaggaa atatcgactc ttttgataca tctgctatat
     6781 ttagcaaaga ttcacaggat gctatcatca tggaaagcaa tataaaagct gacaaaacta
     6841 tcaaactgcc tactgctgat ctaagaaacc aaatctatga cctgatggaa ggaactacta
     6901 agttggcaaa tagctgatat gatgaaaaga tagcaaatac tacttctttg aatagcattt
     6961 gcttaaaaaa agtactcgac taaacgtagg aaagagtgtg ggacaaatct aaaaatcaat
     7021 ttttgctaca gactttaatt ccaaataaac agaacagaaa gaggtgttct tctttctgtt
     7081 ctgtccaaat tattctaaga tttctcttta ccaacattat ttgacaaagg gcctattaaa
     7141 tctaaaatag tatctttaga ttgctaaaat atgtgctagc aaatcattca tggcttgttc
     7201 ttatttttaa ttttatgcaa taaaaaagaa gaacaattag cgtgattata gaaataaata
     7261 atgagatttt agaaaaaaat gtgtatgaat acctgattgt tactttattt attccacttt
     7321 cactttttac tgtaacaaaa ggacctcctt gttttagtac tgtagcattt tttgaattaa
     7381 cagtaaccgt taatccttta taccggaata caggtaaaac tattgttttg ccttttgaag
     7441 atttaaacgt aaatgtaatt tcatctttat ttggagaaaa gctactataa ataacttgac
     7501 catcttctgt aacttgatgg tttttcaaaa gcaattgatc ttttttatag cctataggta
     7561 cataatcaaa attctcaaat tcttcaagtt tattttccat ttgttcatta gaataagttc
     7621 cttgaaaatt catttctttc accatgttaa ctactgatga ataatttaaa attgaaatag
     7681 ctaatattaa taatagtata aaatttcttc tccttgtata agaaaatttt tccaactgtg
     7741 gagaaataaa tagagatact gaaaaagata tgaacaatgt tgaaaatatc ataaaacgcc
     7801 aaggagcttg aataatattt aaaggagtat tttgtaaggc aaaccatgga aatatttttg
     7861 tacaaagtag aatagatatt atacccatta tataagtgat tttagataag attcctaatt
     7921 ttttaaagtt aaatgttagt ataattaaac aaataagcaa agttaagcca ataccaaatg
     7981 atagtaattc tgaatctata cttccaatca aaaactcttt acccagataa attgctcttt
     8041 tttgtaatat atccacataa ggtctattca gatcttgata caatgtctgc tcaatcatag
     8101 gacctataaa cgctagtgaa agaagtaaag taactatagc agcttttatt aatttaataa
     8161 ttaagaagat ttctattttc tttttccgat aaaattgaat aattaaagca attataatca
     8221 ttaccatagt catagctaca gaaagaacat gagtgtaaat taataaagtc atgctaatcg
     8281 ccaacataac ccaagattct ttttttgagt cgtaaagctt tataaacccg agtaaaataa
     8341 gaggaagtat tgacattgcg agtaattccc ccattgcccc tcgaagaaat atttctacac
     8401 tcctaaaaga agatgttgta tatataatag cgaaaaagga gctagtaaca tgttttttgg
     8461 taatttcata cattacataa tgacatataa atagagtaat gagcgtaaca atataaagaa
     8521 aaaggttata gcctattgct aaattagtaa acttgatcaa taaaaataaa ggatatagag
     8581 ttaaccatgg gtaaaaatta tttacttgca tgccatatga tctaaacgtt tcaaaattaa
     8641 taggggagct aaaaatagat tctaaactta acgcacgatt aatgtgaaat gccgtatcat
     8701 aaaagccaaa tatttccatg tttttaaatg ctggaaataa atataataaa ctaacaaatg
     8761 cataaactat aaatattttt acagaaaaaa tttttttcaa attcatatac cacctcatgt
     8821 ttttttatat tacatatagt atcatttatt tttttaaaac actagattag tcactatata
     8881 aaatgtgatt aattttatta ttaataaaat attttttata ttacaaaagt agtgttt
//
LOCUS       DASHFF010000093         8807 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.093, whole genome shotgun sequence.
ACCESSION   DASHFF010000093 DASHFF010000000
VERSION     DASHFF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.093"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..242
                     /locus_tag="U2I75_002562"
     CDS             <1..242
                     /locus_tag="U2I75_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEN1697623.1"
                     /translation="KLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRH
                     SLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            309..803
                     /locus_tag="U2I75_002563"
     CDS             309..803
                     /locus_tag="U2I75_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1697624.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            859..1497
                     /locus_tag="U2I75_002564"
     CDS             859..1497
                     /locus_tag="U2I75_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697625.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1569..1964
                     /locus_tag="U2I75_002565"
     CDS             1569..1964
                     /locus_tag="U2I75_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1697626.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            1948..4401
                     /locus_tag="U2I75_002566"
     CDS             1948..4401
                     /locus_tag="U2I75_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1697627.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4398..6425
                     /locus_tag="U2I75_002567"
     CDS             4398..6425
                     /locus_tag="U2I75_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697628.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6422..7444
                     /locus_tag="U2I75_002568"
     CDS             6422..7444
                     /locus_tag="U2I75_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN1697629.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7461..8390
                     /locus_tag="U2I75_002569"
     CDS             7461..8390
                     /locus_tag="U2I75_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1697630.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            8635..8751
                     /locus_tag="U2I75_002570"
     CDS             8635..8751
                     /locus_tag="U2I75_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN1697631.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2979 a         1480 c         1759 g         2589 t
ORIGIN      
        1 ctaaattata tagtaggata caacaattaa ttgaaaaaga aattcaacct actgaacttg
       61 ctgatatagt gaagacaacg ataaggtcat caaatggaat gattagctct cttaatgaag
      121 caagaaatag gcattcactt gcacacccta atacaaatat tattggaaag agagaagcaa
      181 aactaataat aggtatttca agtacagtta cagattatat tagcggatac cttgataagt
      241 agcgtcatta actgattgct acttttttta tgactatttt tataaaaagg atgtgataag
      301 aattggaaat gaaagtctat gtggcaaatt taggacggta taatgaaggt gaattagtcg
      361 gagcatggtt tactccacca attgatgagg aagaaatggc agaaagaatc ggtttaaatg
      421 aggactacga agaatatgca atacatgatt ttgagcttcc ttttgatgtg gacgaataca
      481 cgccgattag tgaaatcaac cgtctatgtg aagccattca agaaatcgaa ggtacaccta
      541 tttataatga actcaaagaa attcaaggca tgtggtttag tagcttggaa gaattactgg
      601 aaaacaaaga ggatattcat tgttatagtg attgtgattc gatggaagat gtcgcccgtt
      661 attacgtgga agaaacagga cagcttggtg aagtcccttc caatttacaa aattatattg
      721 actaccaatc tttgggacgt gatatggaaa tagagggtaa ttacctagtt acctctcatg
      781 gggtatttga atattgtcag taagtttaac aatccgtgtt ataattaaat taataaatag
      841 cacggagagg tataaattat ggtaaatgta tttgaaaatc ttaaactatt aaaactagac
      901 atgaaagata aaggctgggt gatagattcc ttttactttc gttacaaaca gcaaaattat
      961 attgtacttg ttaagctatt tgaaaaagag gaaaaagttc cagaatatgc tctgttaaaa
     1021 ctagaatttt taaaggaaaa cgatttttca gatatgctag ctgtctatgc taattcggta
     1081 aaattatata cagacacgaa aacaataaga gaatattttg gtatcgaata cagtagtaac
     1141 ttgggagatg ttttatttca atttagccaa actcttgctc gatttattcc gacagaagtt
     1201 agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt tctcattaag tcaaagtgat
     1261 tcagaagatc ccagaaaaaa atattgtttt tcagttagaa gaaatccttt aaagggaaat
     1321 ggtgagctgg gaaaacgaag tccttttaat gacaataaaa cgagattgta tagacccagc
     1381 ttatatgaac gattagggtc agataccaat ttaagtttca gatattcaat gaatccagat
     1441 gatgaagaaa ctgatgaagg tattattgca aaatggacaa aaaataaaat tgaatagtta
     1501 aagcattgaa agagcaatca cctattttga ttgttctttt tctattttta cggaaaggac
     1561 gtgaagacat gaaaaaaata agaagttaca ccagtatttg gtcagtagaa aaagtgattt
     1621 atgcgattaa tgatttacaa ctccctttcc caattacctt tacgcaaatg acatggttta
     1681 tcgtttcttt gtttgtcgtc atactctttg gcagtttacc accactttct ctgattgacg
     1741 gagcactact caaatactta gggattccca ttgttctcac tgggttcatg tgccaaaaga
     1801 cctttgacgg aaagaaaccc tatggatttc taaagtctat cattacctat gctctacgcc
     1861 ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa agaaacaatg aatgaatcga
     1921 ttacgattgt aaggagtgaa caactagatg gcatatccga ttaaatatat ggaagataat
     1981 ctcgtattta atcatgatgg cgaagtattc gcttattatg aattaattcc ctataattac
     2041 agttttttaa gttccgatga aaaaattcaa gttcatgata atttctgtca attgattgcc
     2101 caaaatcgtg atggcaagat tcatgctctg caactagcaa ccgaatcaag tattcgagcc
     2161 acacaggaac aatcgaaaaa agaaatcact ggacgattaa aagagatagc ttacgaaaaa
     2221 atagatgaac agaccgaagc gttaatttct atgattggtg acaatcaagt agactatcgc
     2281 ttttttattg gttttaaact ctacctcaat gaacaggaag tatccgtaaa aaatgttggg
     2341 aaagaaattc aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa attaatgggc
     2401 gattttgttt ctatgcctat ggcggaaatc gaacgctttt ctaagatgga acagttgtta
     2461 gaaagtaaat tatccagacg ctttcagatt cgtaaattgg agaaagatga ttttggttat
     2521 attattgaac atctttacgg gcaaacaggt gtcccttttg atgactacga ctaccattta
     2581 ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat acgatattct caaacccact
     2641 cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg aacaggaaga taccactact
     2701 tatgtggcgt attttacaat taatagtatt gttggtgaat tggatttccc taatagtgaa
     2761 attttttatt accagcaaca gcagtttact ttccccgttg ataccagtat gaatgttgaa
     2821 attgtcgcca ataaaaaagc cttatctacc gttcgcaata aaaagaaaga actgaaagat
     2881 ttagatagcc acgcatggga aagcgacaac gaaaccagta ctaatgtggt ggacgcttta
     2941 gaatctgtcg atgaattaga aagtaattta gaccaaacca aagaatctat gtacaaacta
     3001 agctatgtgg tacgggtatc agcaaagaat ttggacgagc tgaaacaacg ttgtgatgaa
     3061 gtaaaagatt tttatgatga tctgtctatc aaattagtac gaccttttgg cgatatgcta
     3121 ggcttgcata atgaatttat tccctcaagc aagcgatata tcaatgacta tattcaatat
     3181 gtcacgtcgg attttttggc tggtcttggt ttcggtgcta cacaaatgat tggtgaaaat
     3241 tctggtattt acgtcggtta caatctcgat acaggcagaa atatttatct ccaacctagc
     3301 cttgcaagtc aaggaattaa aggctctgta acaaatgcat tagcgtccgc ttttattggt
     3361 tcattaggtg gcggaaaatc attctctaac aatatgctgg tctattattc agtactcttt
     3421 ggcggacagg caaccattgt tgacccaaaa gctgaacgtg gtgaatggaa agaaacctta
     3481 cctgatattg cccatgaaat caatattgtt aatctcacga gtgagaacga caataaaggg
     3541 ttacttgacc cttatgtgat tttaaaactc ccgaaagatt cagaaagcct tgccattgat
     3601 attctcacgt tcttaacagg tatttccagc cgagatagcg agaagtttcc tgtcctgcgt
     3661 aaagctattc ggagtgtaac tcaaagcaaa caacgagggt tgttattggt gattgatgaa
     3721 ttgcgaaaag aagatacgcc catcagcaat agtattgctg accatatcga aagctttgtc
     3781 gattatgatt ttgcccatct gttattcagt gatggcagtg tcacccaatc cattagcttg
     3841 gaaaaacaac tcaatattat tcaagtggca gatttggtct tacccgattc caatagctct
     3901 tttgaagaat acactaccat ggaattatta agtgtggcaa tgctgattgt cattagtacc
     3961 tttgctttag actttatcca ctctgaccgt tccattttta aaattgttga ccttgatgag
     4021 gcttggagtt ttttacaagt ggcacaagga aaagctctat ctatgcgatt ggttcgagca
     4081 ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa acgcggacga tttactagat
     4141 gaaaagatga agaataatat tggcttgaaa tttgcctttc gttccactga cctagtggaa
     4201 atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag atgaaaacaa tcagaaacgc
     4261 ttacgtgaat tagaaaatgg acaatgttta gtttcagaca tctatggtcg tgtaggggtt
     4321 atgcagtttc acccgatttt tgaagagttg cttcatgcct ttgataccag accgcctgtg
     4381 cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt tcttcactat agttggataa
     4441 cgctcctttg tgtgggcgtt attttacttt tattaggcac gctgggcaat gcagtacaag
     4501 cgacaggctt agttgatgaa accattgata caagcaatga atactccaag tatggtttga
     4561 atcaatatca gcttgattat tatgtggaca atagctgggg ctggcttcca tggaattgga
     4621 gtgatggcat aggacaatcg gtgatgtatg gattgtatgc cattactaat tttatttgga
     4681 caatttctct ttatctatcg aacgccacag gttatttggt gaaagaagcc tatgccttag
     4741 atttcattag tcaaacatcc caagcaattg gcgaaaacat gcagacgatt gcaggcatta
     4801 caccgaatgg cttttccagt tcagggtttt atgtcggttt cctccttctt tttattctca
     4861 ttgttggaat ctatgttgcc tatacaggat tggttaagcg agaaacgacg aaagtgatac
     4921 gagctgtttt aaactttgtc gtggtgttta ttctatcggc ttcctttatt gcgtatgcac
     4981 ccgattatat tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc agtctgaata
     5041 ttgggacaaa aattgttctt cctaacacgg atagtcaagg gaaagacagt gtggacatga
     5101 tacgtgacag cttattctct gttcaagtaa agcaaccatg gctattgctt caatatggct
     5161 ccactgatgt agaagcgtta ggggaagaac gtgtggaaag tttattgtct accagtccag
     5221 atacaaacaa tggtgaagat agagaaaatg tagtgattga agaaattgaa gatcatgaca
     5281 atacctattt aaccctaccg aagacgattt ccagattagg aacagtcttc tttttgtttg
     5341 tattcaatat tggtatctcg atttttgttt tccttctaac aggaatcatg attttttcgc
     5401 aagtgctatt tattattttc gctatgtttt tgccaatcag tttcctatta agtatgttgc
     5461 cgagttttga tggcatgggc aggcatgcca ttaccaagtt atttaatgtg attatgacaa
     5521 gggctggtat taccttgatt attaccgttg cctttagtat ttccactatg ctttattcgc
     5581 tgtcagcaag ttctcccttt ttcatgatta tgtttttaca aattgtgacc tttgcaggga
     5641 tttattttaa actgggcgat ttaatgagcc tattcacttt acaaagcaac gactctcaaa
     5701 acatgggcag acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa accagaagat
     5761 ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag caaaagacaa
     5821 tgacaacaaa gcagaatcaa accaataaag ctaaccatac tcgtcaaaat gaacggacta
     5881 caccaaagaa agaaacagca ctttccaatg tgaaaaagca acaaggcggt atcgggcaac
     5941 gattaggtga aaagacagcc catgtattgg acacgaaaaa tcgtctagtc aataaaagaa
     6001 agcaggcaaa acaacagata caggcgacac caacccatgt caaatatgcc cttcatcaag
     6061 gaaaagagaa tgtcactcat aatgtaacag actttaaaca atctatggca gataccaaag
     6121 ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa acgaaagaac attgctcaaa
     6181 agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca tcatctcctg
     6241 ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct gttttgtcac
     6301 aaaacactaa acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa caaaagacac
     6361 caccgaagca attcaatcag aggaagctaa ataaaagaac ggataagcga ggacaacaaa
     6421 catgaagcta aaaataacag tgatttgtag tgtggtcttc tctctcttct tttttctctt
     6481 attattccta gtcattttct ttgcagatga tacggacagt ggcgaaaaca acaaggattc
     6541 ttctattcca caaggcggtg tgaccgtttc accagaagtg ttggcacacc gtccgttgat
     6601 tgaaaaatat ggtaaggaat atgggattga agactatgtt tcttatatct tagccattat
     6661 gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa agttccgaat ctttgggttt
     6721 accgcccaac agtttgagta ccgaagagtc catcaaacaa ggggttaaat atttcagtga
     6781 attattaacc agtgcagaac aacaaggagt ggatatagat tctgttatcc aatcttataa
     6841 ttatggcggt ggctttttaa attatgtgag gagtcatggg aaaaaatata cctatgaatt
     6901 agccgaacag ttttctaaag agaagtcggg tggtcaaaaa gcagactatc ccaatcctat
     6961 agccatacct gtgaatggtg gttggcgata taactatgga aatcagtttt atgttcagtt
     7021 ggtttcacaa tacctaaccg acacttcccc aacagaattt gatgatgaaa ccgtgcaagt
     7081 cattatggac gaagccttga aatacgaagg gtttccctat gtctttggag gggcgtcgcc
     7141 taccacctct tttgattgta gtggcttaat acaatgggtg tatgacaaag ctggcatttc
     7201 tctccctcgt gtcgcccaag atcagtatga cgctacgcaa gaaatctcta tggaagaagc
     7261 ccaagcaggc gaccttatct tctttcattc aacctataat gcaggcacat atgtgactca
     7321 tgtggcgata tatttagagg gaaatcgctt ttatcatgca ggcgacccaa tcggttatgg
     7381 cgacttaagc agtcgttatt ggcaagacca tctgattggg gctagacgtg ttattcataa
     7441 ctaagaaagg aatgaatcaa atgattataa aaatcgagcg aaaagaaaag aaggagaaac
     7501 caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa gttggcacgc
     7561 ataagaaatt aacctttgtt ttatgggttt tattgattgg tagtgtcgga tttggaatct
     7621 ataaaaattt tactgccatt gacactcata ctgtccgtga aacagaaatc attaaacagc
     7681 agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa gactactttt
     7741 cgtggcaaca atcccaagaa gccattgata aacgaaatga aaaattgacc cattatttga
     7801 cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa ggatattcct actagttcta
     7861 gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc tttgaggtac
     7921 tgttttctgt tgaacaagtt attaccgaag acaaagacaa ggaaactata tcgtctagct
     7981 ttcatgtggt tgtccatata gatgaatcag ataatatggt tattatcaaa aatccgacaa
     8041 tgagtaagaa acctcaaaaa tcggactacc aacccaaaca gctagaaagt gaccatacag
     8101 tagatacaga aacgatggac gaaatcacta gctttttaga aacgttcttc cagctctatc
     8161 ctaccgccac agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta cctatgatta
     8221 acaaggaata tgttttcgag gaattggtga atcctatctt taccagaaaa gataaccaag
     8281 taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc caaatctcgc
     8341 aatttgagtt gatattagaa aaacaagata attggaagat tgtaaaataa caaatattgg
     8401 tacatgatta cagatacttt gtaatcatgt actctttttg ataaaaaatt ggagattcct
     8461 ttacaaatat gctcttacgt gctattattt aagtgactat ttaaaaggag ttaataaata
     8521 tgcggcaagg tattcttaaa taaactgtca atttgatagc gggaacaaat aattagatgt
     8581 ccttttttag gagggcttag ttttttgtac ccagtttaag aataccttta tcatgtgatt
     8641 ctaaagtatc cagagaatat ctgtatgctt tgtataccta tggttatgca taaaaatccc
     8701 agtgataaaa gtatttatca ctgggatttt tatgcccttt tgggtttttg aatggaggaa
     8761 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgc
//
LOCUS       DASHFF010000094         8461 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.094, whole genome shotgun sequence.
ACCESSION   DASHFF010000094 DASHFF010000000
VERSION     DASHFF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.094"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="U2I75_002571"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="U2I75_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN1697632.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="U2I75_002572"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="U2I75_002572"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN1697633.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="U2I75_002573"
     CDS             2591..3376
                     /locus_tag="U2I75_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1697634.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="U2I75_002574"
     CDS             3389..4240
                     /locus_tag="U2I75_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN1697635.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="U2I75_002575"
     CDS             complement(4364..4816)
                     /locus_tag="U2I75_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697636.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="U2I75_002576"
     CDS             complement(5281..5649)
                     /locus_tag="U2I75_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN1697637.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="U2I75_002577"
     CDS             5799..6281
                     /locus_tag="U2I75_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN1697638.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="U2I75_002578"
     CDS             6318..7328
                     /locus_tag="U2I75_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN1697639.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="U2I75_002579"
     CDS             complement(7443..8156)
                     /locus_tag="U2I75_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697640.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            8306..>8461
                     /locus_tag="U2I75_002580"
     CDS             8306..>8461
                     /locus_tag="U2I75_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1697641.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSG"
BASE COUNT         2877 a         1373 c         1714 g         2497 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtcta tggataggct acaattaact agacagaaaa attaaggtgt gtagactaga
     8281 agaaaacata ccaggaggaa tttttatgtc taagagaaca cgaagaactt tttcacaaga
     8341 attcaagcaa caaatcgtca atctttactt agctggaaag ccacgtgtag aaatcattcg
     8401 agaatatgaa ctaacggctt cagcatttga caaatgggta aagcaatcta aaacgagtgg
     8461 t
//
LOCUS       DASHFF010000095         8385 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.095, whole genome shotgun sequence.
ACCESSION   DASHFF010000095 DASHFF010000000
VERSION     DASHFF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.095"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(82..1233)
                     /locus_tag="U2I75_002581"
     CDS             complement(82..1233)
                     /locus_tag="U2I75_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN1697642.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(1387..1917)
                     /locus_tag="U2I75_002582"
     CDS             complement(1387..1917)
                     /locus_tag="U2I75_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1697643.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(2016..2366)
                     /locus_tag="U2I75_002583"
     CDS             complement(2016..2366)
                     /locus_tag="U2I75_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN1697644.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(2523..4052)
                     /gene="ahpF"
                     /locus_tag="U2I75_002584"
     CDS             complement(2523..4052)
                     /gene="ahpF"
                     /locus_tag="U2I75_002584"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN1697645.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(4062..4625)
                     /gene="ahpC"
                     /locus_tag="U2I75_002585"
     CDS             complement(4062..4625)
                     /gene="ahpC"
                     /locus_tag="U2I75_002585"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN1697646.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(4769..5431)
                     /locus_tag="U2I75_002586"
     CDS             complement(4769..5431)
                     /locus_tag="U2I75_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1697647.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5615..7261
                     /locus_tag="U2I75_002587"
     CDS             5615..7261
                     /locus_tag="U2I75_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1697648.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILATTADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            7252..8334
                     /gene="uxuA"
                     /locus_tag="U2I75_002588"
     CDS             7252..8334
                     /gene="uxuA"
                     /locus_tag="U2I75_002588"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1697649.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         2759 a         1611 c         1502 g         2513 t
ORIGIN      
        1 gactgtggcg ttatcgtcac agtccttttt tcaaataaac ggttttcacg atgtctcttt
       61 cacaacttct ttttgattta ctcacacttc ataaactttt tttgtaaaat atttgagaac
      121 aagtggagaa ataagtgtac ttaataaaat cacgataact aaaggtgaat agtactgtgg
      181 ttcgatcaat tgactttgct gtccaatttg taaaatgatc aaagccattt ctccacgcga
      241 aatcatacca gcaccgacca tcaatgaact atgttgagaa aaaccggcaa tctgagcacc
      301 aaaataacct ccaagcaatt tggtcaaaat cgctacaagc gtcaatatag aaataaagag
      361 tagttgttca ctaaagtcag aaaaatcaac ttctaatcct acactgacaa aaaatactgg
      421 tataaatact gcatatccta aagcctctac attgtggtat acttcttctt taacctttgt
      481 ctgactgaca gctatcccag caaaaaaagc accaatgaca gagctaagcc caacaagatc
      541 cgctacgtaa gacattccta aacaaataac tagcgatacg atgataatag aagaatttgc
      601 gtacactttt tctgcaaatg aaagtaggaa aggcgcaatc catttgacta ataagaaaat
      661 aaaaacaaaa taaattagct gttcaatcaa aacaagcggt aaagaagcat tgctagaagc
      721 ttcccccgtt agaaaagata ggctgaaact tagaactaaa acgactaaaa tgtcatctac
      781 cacagaagca cccaaaattg ttgatccttc ttttgtattc actacgttta gttcttttaa
      841 tacttctact gaaatactga cagaagttgc cgcaagaata atcccaaaga aaatcgcttc
      901 atttcccgaa acttggaatg ctttgcctgt caaccagcca aaaacaaccg ggaagatgat
      961 cccaagcaaa gcaacgaaca taccaggtcg gaaatatttt ttcaaaagag ataaatcact
     1021 ttccagacca gctaaaaaca ttaataagat cacaccaatc tccgaaaagt catggactag
     1081 aatatcaggt ttgacccaat tcaatccagc ttggcctagc aaaacaccaa caagtaattg
     1141 tccaatcact gctgggatac ctaatctccg cgagatatgt gagccaatcg ttgttgaaat
     1201 caatatcagg cataaaatac caataaattc cattctcatc attcctttgt ataaaatata
     1261 gcccgaactt agcataacat acattattga aaaaatagga aaatatactc acaaaaaaac
     1321 caacaatcca aaagattttt ttggattgtt ggtttttata cggtctattt taataactaa
     1381 aaatgattaa cgtatagtca tttttcttat tctgatgaaa ttgataatct cctggatgcc
     1441 catgaaaatc aaaataaagc cgaatatttg gaatagaagc atcaaacttt tgaatggatt
     1501 gaacaataag acgaatccgc caatcaacaa cagaatacta ctataaacac tccaactttt
     1561 agcttttgta ttcatgctga agaacaactg gaacaagctg ttgatgataa cagctaatcc
     1621 aagaagaatc ggcaggatag aaacgattgc aggtgcaaaa gcatagacga tcagagctaa
     1681 gatcaggaaa aaaagaccac tgaacagacc gatactccaa gaacctgttt tccttttaaa
     1741 acggtaagcc tcaaaaagat tcaacaaccc aagcaaaaca agataggcgg tgatcagata
     1801 accgataaaa tgaaaaacag cagaagggtt tagcacaatc gcaatcccag aaataatata
     1861 aacagcagct cttaataagg cgtatttttg aatattttgt ttaatcgtct caaacatata
     1921 agaaaacctc ctttgactta ttttaacgtg aactagtcat tttaccaaat taaatgttac
     1981 aaaatgagcg aaggagaaga atatctctgg aaaaatcaac ttctgaggat cttttccatt
     2041 tttttgcctt ttgctagctc atcgatcatt ttatctaaat aacgaatttc tttcatcaag
     2101 ttatcctcga tgtcttctac tcgtacacca caaacaacac ctgtaatcaa ggaacgtgaa
     2161 ggattgggga caggggcttc cgcaaaaaat tgttcgaaag tcacttgttt ttgtagttga
     2221 tattctagtt ctgtttggga ataacccgtc agccaacaaa tgatttcatc tacttcctct
     2281 tttatacgat cttttctctc cgcttttgct acatacatag gataaacgct tgcaaaactt
     2341 gtcgtaaaaa tacgattttg cgccataatc agtcaccttt ctttttttca aattatttac
     2401 tacttatagt atataagaaa aagagaagaa aaagcgatta ttcaaaaaaa caaaaaggat
     2461 tgtgacaaaa ttcttgtcac catccttttt tccgctctct gactttttac aatcttcata
     2521 gctcaagtgc gcatcagata atcgaatgct cctaaagctg cagtagctcc tgaacccatt
     2581 gaaacaatga tttgtttgta tggactatct gtacaatcac cagctgcgaa aacgccgggg
     2641 atattcgttg cgccattttt ctcggtcact atttcatttc ttggattcat ttcgatcgtt
     2701 ctatctagcc aatccgtatt agggagtaag ccgactagaa tgaatactcc tgaaacagct
     2761 agtgtatgca tctccatcgt acggcgatcg atataggaga gagattctac ctgattcgta
     2821 ccgctgattt ctttcgtcgc tgcatgtttg ataacagtga cattcgcaag agagactagt
     2881 ttatcctgta agatttgatc tgcttttaat tctggtaaga attctagaac atatacatgt
     2941 tttgctaacc cagccaaatc gattgctgct tcaacaccag aattaccacc accgataaca
     3001 gcaatctctt ttcctttaaa taaagggccg tcacagtgtg ggcaataagc aattccttta
     3061 tttttaaact ctttttcgcc tggtacattg atagaacgcc agtgcgcacc tgtcgcgatg
     3121 atggcggttt ttgctttaag tgatataccg ttagctaagc caatattaat aaaatgttgc
     3181 tctttattta aggaaacggc ctgttgattc atcataatgt cgatagggta actacgcaca
     3241 tgactttcta cttgctgcat caattttgga ccttctgtgt aagaggtacc aatcatattt
     3301 tcaatcccta gtgtttcaac gacttgtccg ccgaaagaat cagcgacaag tcctgtacgg
     3361 attcctttac gtgctgcata gatagctgca ctactagctg ctggtccacc gccaatcact
     3421 aatacatcat aaggttcttt ttcaaacaac tcttcttcaa tcaaagggcc agtgattttt
     3481 tcaacaattt gttcgatcgt catgcgtccg ctactaaatt cttctccatt taaaaacaca
     3541 gttggcactg ccatgatttt tttatcatcg atttcttttt ggaacatccc accttcaatc
     3601 atcgtatgag taatatttgg atttaataca gctaatatat tcaatgcctg tacaacgtcg
     3661 gggcaattat gacaagtcaa gctaacataa ctgtcaaaat gcaatgtttg tgttattttt
     3721 ttgattttaa gactcaaaga ttcactgatt ttcggtgccc gtccactaat ttgtaacata
     3781 gccagtaaaa aagaggaaaa ttcatgaccg agggggatac cagcaaaaac gattccactt
     3841 tctttgccaa ctggattgat agaaaaacta ggtgtgcgca tcgaatctct tttttcccaa
     3901 gagattttag gagacatttc tgctatttcc ttcgtaaatt cagctagttt ttgactattt
     3961 tcactattat ccaaactaac agaaaaaaca ataggagatt caatcagtgc taaatattgt
     4021 tcgagatttt gtttagtttc tttgtctaac atatccacca cctaaatctt tccaacaaga
     4081 tcaaaacttg gtttcaatgt ttcgccactt tctttccatt ttgccggaca aacttcacct
     4141 gggtgggtac gtacatattg agcagcgcga atcttatcaa tcagtgcact agcgtcacga
     4201 ccgattccat ctgcattgat ttccatagct tgaacgattc cgtctggatc aataataaat
     4261 gttcctcgct gagctaatcc ttgctcttca tccaaaacat caaaaatacg agaaatctga
     4321 tgagacggat caccgatcat cacgtattcg attttaccga tagcatctga tgtatcatgc
     4381 catgctttgt gagtaaagtg ggtatcggta gagcaagaaa agacttcaac acctaaagat
     4441 ttcaatgtac tgtattgttc ttgtaagtct tctaattcag taggacaaac aaaagtaaaa
     4501 tcagctggat agaagcaaag aatactccag ttccccataa gatctttttc actgacttct
     4561 aaaaattctc cttgatgata agcatgtgca ttaaattctt cgattttttt cccaatcaat
     4621 gacatagtaa aacctccaat aataattatt tatttataat tattgtaatt tagttggagt
     4681 tgcaaagtca atggatattt tgttatttaa taaagattac tcaaaaacga aggttaatta
     4741 taaaaataga agaggtcgca agaaaatgct actgctggaa atactcggga taacgttgag
     4801 taacagtttc ctgttctttc agcatcatgt gtaagtgcaa tcccgtttga aatcgagctt
     4861 cttctggttt tttatcaact aaagccttga gtaaatcata atgttgttcc atgatcgttt
     4921 cccatggaag cccctgaatg gtcactcgta accagcggaa acgttctaaa tgggtattat
     4981 ggtcatctaa ccagttccaa acttcttttc ttcctgcaat ttcgaaacaa gcttcatgaa
     5041 ataaattatc tgcacggaaa aaagcgcgtt cgtcatttat tttgactgct ttttcttgtt
     5101 cttctaaaat cgtttctagc actattcgtg tttgtgtgtc caatttggaa caacattcaa
     5161 tcatgatttg ctcttccaac tgttctcgga caaagcgagc attttctgca gaaacaagat
     5221 taatttttga aacatacgta ccactttgag gtatcgtata cacgagttct tgctgacgta
     5281 gttgaatcaa tgcttcgcga atgggagtcc ggccgatagc aagatgttcc tccagtgcct
     5341 tttcagatat tttccttcca ggttctaagt cagcatagat aatttgttta cgaatcgctt
     5401 gataagcttg agattgcaag ctgcttttca tctttattcc tccaaaatca tttctatgaa
     5461 cgtagtataa cagaagaggt ctgaaaaaca atctggtatg tcacattaac tagtaaattt
     5521 gtataaatag taaaattttg gttgacatag cgatttttat tgcataaaat tcacttgaat
     5581 acaaaaacta atatgttagt ttaggagggg aaaaatgatt gatttaaaca actggaggaa
     5641 ctttatccct gaattatctc aaaaagaagt agaaatacct cattttgacc gtgcatcatt
     5701 aagagaaaat ggacaaaaaa atcctgtctg gatccatttc ggtggcggaa atctttatcg
     5761 aggatttcat gcagaaatcg ctcaacgttt agcggatcta ggcgaattgt ccactggtgt
     5821 cattgtttgt gaaacattcg atgaagaaat aattgaaagg gcttaccaag attttgatga
     5881 tttgattctt gaagtggtca tgccaagcag tggagagttc gtcaaacgta tattagctac
     5941 gacagctgac agtctgtatt gtcatccaga ttgttctgaa cactttgata agatgattcg
     6001 acactttgaa aatcctagtt tacaattagt tacttttgct attactgaaa aaggttatcg
     6061 attgaaagat acaaataata aatatatagg accgattgca gtcgatataa aaaatggtcc
     6121 aaagaagcca tcacatacaa tgagtattct tgctgcgcta ctttataaac gttttttatc
     6181 tggagcttgg ccgattgcac ttgtctctac agataatttt tcacaaaacg gtagtcaatt
     6241 acgtacttct ttgctaacga tcgcaaaaga atggcaaaag gaaggttatg tacaaaatac
     6301 atttatccaa tacttatcga atgaagaaat tgttgcgttt ccatggacga tgattgatcg
     6361 tatcaccccg aatccttcct tgtcagttag caaaatgttg gcaaaagagg gcttttcaaa
     6421 tatgtcgatt ttccaaaccg caaaaggaaa taatattgct ccatttgtca atacagagga
     6481 catccattat ttaatcatgg aagatcattt tcccaatgga cgtccgaaat tcgaaaaagc
     6541 tggagtattc ttgactgatc gtgcaacggt agataaagca gatacgatga aggtgacgac
     6601 ttgccttaat cctttgcata cgtctttagc tattatgggt tgtttactgg ggtataggag
     6661 catagctgag gagatgaaag atccagattt atcgctgcta gtttctgaaa tcgggtacaa
     6721 tgaaggcctc ccagttgtgg agtctccagg tatcattgat ccaaagatct ttctggaaga
     6781 agtaattact gtgcgctttt ctaatccaaa tattcctgat atgcctcagc gaatcgccac
     6841 ggatacctct caaaaggtgg cgattcgtta tggtgagacg attaaaaaat atatggaaat
     6901 agaagttaaa gacccgcaga cacttgtatt cataccattg actctcgctg cttggcttcg
     6961 ataccttatt ggcattgatg atcaaggaaa accattcatc ccgagtccag acccactatt
     7021 gacagacttt cagcataagc tttccgatat tcaactgggt acgcaagata catcacatat
     7081 tcatgagaaa atggctccta ttttgtcaaa taaagaaatt tttggtgtag atctttacca
     7141 agcagagctt ggcagaaaaa tagaagaaat gttcatcaag atggtagcag gaccgaatgc
     7201 cgtacgaaaa acattacagg attacttaca agaacatgga ggaaatttat catggaaatg
     7261 acttttagat ggtacggatc acaggaagaa aagattacct tagatgaaat cagacagatt
     7321 cctgcggtca aagggattgt aggcacattg atggatatac cagtaggaga ggtatggcct
     7381 agggagagga tcaaagaact aaaagaagaa attgagtctc gtgggttaac actaaaagta
     7441 attgaaagcg tcaatgtgca tgatgccatt aaaattggat taccttcccg tgagcagtat
     7501 atcgaaaact ataagcagac aatccgaaac ttggcagaat atggaataga agtcatctgc
     7561 tataatttta tgcctgtatt tgactgggtg aaatctgacc ttgactaccg cttagaagac
     7621 ggctcatcca cgttagcttt tatatctgat gatattccag atgatccaag agaaatcgtc
     7681 gcgcgcatcg aacattccag caatgaattt gaattgccgg gatgggaacc tgagcgctta
     7741 gctcatatca aatccttgct tgaagcctat gcatcagtag acgaagaaaa acttcgagaa
     7801 aattttgcct attttctcca atcaatcatc ccaacatgtg aagaggtagg ggtcaaaatg
     7861 gctgtacatc cagatgatcc gccatactcg atttttggat tacctcgaat cgtaaagaat
     7921 cgagaagatt tagattggtt gtgccgcata gtagacagcc cttataacgg aatcactctt
     7981 tgtacaggga gtattgcaga agatccagaa aatgatgtat atgggatttt agcagaattt
     8041 tgtcaaagag atcgaatccc atttgcacat gttcgaaata ttaaattttt agctaatcga
     8101 gatttctatg aggcaccgca taaatccgaa tacggttcat tagacatgta ccgcatacta
     8161 aaaacaatgt atgacaacgg atttaacgga tacatccgtc ctgatcatgg aagaatgatc
     8221 tggaatgaga caggaagacc aggatacggc ttatatgatc gagcattagg tgctgtttat
     8281 ctaaatggat tatgggaagc aatcgaaaaa ggaaacaaaa gtaaagataa ttagtgaaaa
     8341 aaactgtaat tttattatgt aaataagaag gatgttcaaa ttctt
//
LOCUS       DASHFF010000096         8276 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.096, whole genome shotgun sequence.
ACCESSION   DASHFF010000096 DASHFF010000000
VERSION     DASHFF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.096"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..382
                     /locus_tag="U2I75_002589"
     CDS             <1..382
                     /locus_tag="U2I75_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HEN1697650.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="U2I75_002590"
     CDS             complement(776..1783)
                     /locus_tag="U2I75_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1697651.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="U2I75_002591"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="U2I75_002591"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN1697652.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="U2I75_002592"
     CDS             3679..4002
                     /locus_tag="U2I75_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1697653.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4013..5695
                     /locus_tag="U2I75_002593"
     CDS             4013..5695
                     /locus_tag="U2I75_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN1697654.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            5901..6859
                     /locus_tag="U2I75_002594"
                     /pseudo
     CDS             5901..6859
                     /locus_tag="U2I75_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6869..7105
                     /locus_tag="U2I75_002595"
                     /pseudo
     CDS             6869..7105
                     /locus_tag="U2I75_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            7320..8072
                     /locus_tag="U2I75_002596"
     CDS             7320..8072
                     /locus_tag="U2I75_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1697655.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
BASE COUNT         2686 a         1266 c         1684 g         2640 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gctttttaac tgctatgcca gctattctat tttcaagtat
     4141 ttttattatg attgcggctg ttccagaagt atttaacgtg acgttaccag aggcattctc
     4201 gacgtggctt tggaaagttt atggttattc aatgggatta gttggtcttt tggttgttgc
     4261 tacaacagct cgatgtttag ctaattcaat gaaccgtcaa ttatctgatt caggtaagca
     4321 gattaatgaa gtttctgtta tgttagcgtc aatttgtggt tttgtaatga tggcagctga
     4381 tcaaattgat ggtgggatag ctactgatta tttagggaca aagggattat tagcagcttt
     4441 cgtttctgcg tttgttactg taaatgtgta taaattctgt gttaaaagag atttgacgat
     4501 ccgtatgcca aaagaagttc ccggaacaat cacacagatg tttagagata ttatcccctt
     4561 ttcaattgca gtatttattg ccgttttgat tgatacggga gttcgtcaat tttttggaac
     4621 atcatttgca gcaggtgtaa ttcaattatt gcagccatta tttagtgctg ctgatggata
     4681 tgtcggtatt gcaatcattt ggggagccat ggcaatgttc tggtttgttg gtgttcatgg
     4741 accatcgatt gttgaaccag cgattgctgc aattatttat gcgaacgtag aaactaactt
     4801 tgaattattt aaaaacggtg cacaagcctc aaaagtatta acagttggtc taggaaactt
     4861 tgttggaacg atgggtggta ctggagcgac actagttgtt ccttttctgt tcatgttgtt
     4921 cgctaaatca aaacaattga aagcagttgg taaagcttca tttattccag tttcgtttgc
     4981 ggtaaatgaa ccgttactat ttgctactcc tattattttg aatccgtact ttctaattcc
     5041 atttttatta gcaccaatcg ctaacgtatg gatattcaaa ctttttgtcg atgtcttggg
     5101 gatgaatagc tttatatccg tattgccttg ggcgacgccc gcaccgattg gcttggtttt
     5161 aggaaccggg tttagtgtgt tggcgattgt attagcggta gtattgattg ttgtcgatgc
     5221 gattatttat ttaccttttg ttaaggccta tgattctgaa ttagtagaac aagagttaaa
     5281 aaacattgct gcagaagaag taaaagaaga attggtcgaa gaaatccaag aagttggtgc
     5341 aactgcggca gttatagttg atgaagcaaa agcatcggtt ggtgaaggaa agcgcgtttt
     5401 agtactttgt gctggtgccg gaacgagtgc aatgttggct aatgcattag aagagggcgc
     5461 aaaagagaca ggtgttaatc tttctgctaa agcaggagct tatggttctc attatgatat
     5521 gatgaaaaat tttgatttaa tcgttctagc acctcaagtg aattcctatt tggatgatct
     5581 ccgtaaagat gcagacaaat tagggataaa agtaattgca actaaaggtg cagaatatat
     5641 caatttaaca cgtgatccta aaggagcagt tgattttgta ttgaagcaat tttagagctt
     5701 gcttagatta ataaagcgct agccaactta gctagcgttt tatcttaaac acaatggtaa
     5761 gcgctatctt tttttgctgt ttaaatttcg ggtattcttt ttgaggcgtc gattgtaaaa
     5821 ttaagctaga caactaaaaa gatcatttgt gctacactca aaatagttcc gaccaaagaa
     5881 ttaataagga gtgagtacaa atgacctaca cccatcttac aacagacgaa ctagtaatca
     5941 tagagtctta tttcaatatc gagaaacctg tcgctcttgt cgcacaaagt ttaaatcgct
     6001 ctagacaaac catttataat gtttattcgt ttctcaaaca aggaaaaaag tgctagatta
     6061 ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga
     6121 tgaacaatca acatatgttc aagaaaaggt agctcaaggg tggtcacccg atgtgattat
     6181 cggccgtggc gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa
     6241 aggccttttt gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca
     6301 agaaaagcgt ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc
     6361 acacctgact aaagaatttg gtcatctcga aggcgacacc attgtgggag ttaaacacaa
     6421 gagcgctgtt attacattgg ttgagtgttt atccaaggtt attattactt tgaaaccgac
     6481 aggcagacga gccgaagata tcgaaaacag gttaaatgat tggttcaaat ccatgcctaa
     6541 acacttgttt aaatccatga catttgattg tggaaaagaa ttttccaact ggcgatctat
     6601 aagtaactcg aacgatattg ccatcttttt cgcagatcca ggaacacctt cgcaaagagg
     6661 gctaaatgag cactcaaacg gcttgcttag acgagatggc ttacctaaaa caatggactt
     6721 tagggacatg gatgaaacct ttattcaatc cgttgcctcg aaaagaaacc atattccaag
     6781 aaaatcatta aattatcgaa caccattgga agtatttttg agctgcatag ataaagacat
     6841 attgtctagc ttaatttgac aaatcagaaa agaaaaaatg tatcaagttt ttgaagaaat
     6901 gggttatcca attaaaacgg tcaccagaaa actagaagta atcaaagcgg atgatacgat
     6961 cagcgatttg ttagatgtgg aagaaggaga tccaatgttc tatttccata cacagggttt
     7021 ttcgctagaa cgtctgcccg ttgagtattc gatttcgaaa tatcgaggag acattaacta
     7081 ctttgttttt gaacttgcac aataacttca gattaaataa aatgatttat taaagataaa
     7141 agtcgaatga acaaggctgt cactatgaca gtcatgctcg ttcggtttta ttttttagaa
     7201 tagcagcttg caaattctga aaaattttgc tttccttcct gcagaaggct tcttaagaaa
     7261 acttgttcta gcacttttct taagaatttg atagtcttaa aaagaaaaga ggcgatgaaa
     7321 tgttaaaaac ggatcgattc caagcgatca tgtcgttatt agaggttaac ggtacagtac
     7381 gagtcagcga tattatggag aagttttcgg tttcggatat gacagtccga cgtgatttgg
     7441 acgaaatgga gaaacaagga ttattagaac gcgtccatgg cggagcgaaa ttgaaggatt
     7501 tttatcggca tgtggagctt tctcatagag aaaaacagat tatgctgcaa gaagaaaagc
     7561 agcggattgt ggaaaaggcg aatgaaatga tcaatgatgg tgatacgata tttcttggac
     7621 caggaactac aatcgagctc atgtcaaaaa tcattcaagg ggattcactg cggattgtta
     7681 caaactgttt gcctgttttt gaagaattaa ataagcgaaa agggaatcga aaaatctatc
     7741 tattaggtgg cgagatgcgg gaaacgactc aatgctttgt tggtgaaatc acgaataaat
     7801 cgctacagga tatgaatttt cataaagcat ttttcagctg taatgcttta aatgaacaga
     7861 aaattatgac ttcgactttt gaagagggcc aaactcaaca gatcgctctg aataattctg
     7921 ttgaacgctt tttattgatt gatcattcga aaattgggaa aagagatttt tcagtatatt
     7981 ataatctgaa tgatgtaaca gtagttataa cggatgatga tgaacaaaag cactatgaaa
     8041 aaatcgaaaa agaagaaaaa tatgttgtgt aaatgagaaa ctgccaaact ctagataatt
     8101 agaacttggt agttttttgg tagccttgaa tttaagcgaa ttatgttatt tttagggtgc
     8161 atcattacta attcctccga atacttgaat acaccatttg aaaaaagata gtcgattttt
     8221 ttgggatgtt atgtttatgt gaaggaaaaa atagcagtat caatcaccgc tttatt
//
LOCUS       DASHFF010000097         8067 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.097, whole genome shotgun sequence.
ACCESSION   DASHFF010000097 DASHFF010000000
VERSION     DASHFF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.097"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..884
                     /locus_tag="U2I75_002597"
     CDS             <1..884
                     /locus_tag="U2I75_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN1697656.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="U2I75_002598"
     CDS             932..3061
                     /locus_tag="U2I75_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN1697657.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="U2I75_002599"
     CDS             3036..3725
                     /locus_tag="U2I75_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697658.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4818)
                     /locus_tag="U2I75_002600"
                     /pseudo
     CDS             complement(4133..4818)
                     /locus_tag="U2I75_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(4954..5148)
                     /locus_tag="U2I75_002601"
     CDS             complement(4954..5148)
                     /locus_tag="U2I75_002601"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697659.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5198..5638)
                     /locus_tag="U2I75_002602"
     CDS             complement(5198..5638)
                     /locus_tag="U2I75_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN1697660.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5642..6487)
                     /locus_tag="U2I75_002603"
     CDS             complement(5642..6487)
                     /locus_tag="U2I75_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN1697661.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6636..8054
                     /gene="pepV"
                     /locus_tag="U2I75_002604"
     CDS             6636..8054
                     /gene="pepV"
                     /locus_tag="U2I75_002604"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN1697662.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2480 a         1515 c         1714 g         2358 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaagct tttttatttt gagcgatcac tgcctgatcg gtttgttcta gtattttttc
     4501 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
     4561 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
     4621 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     4681 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     4741 tccgggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     4801 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     4861 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     4921 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     4981 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     5041 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     5101 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     5161 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
     5221 aagcgtacgc ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc
     5281 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     5341 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag
     5401 ttgggtggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     5461 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     5521 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     5581 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     5641 ctcatgcctc gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt
     5701 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     5761 tatgaagata aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     5821 ctgtgatcat cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta
     5881 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     5941 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     6001 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     6061 agggtagcaa aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc
     6121 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     6181 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     6241 cagtatccgt ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     6301 ttttttcatc agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac
     6361 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     6421 ttcctttatg tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     6481 tcagcatcga aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     6541 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     6601 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     6661 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     6721 agcgcgatga ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     6781 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     6841 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     6901 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     6961 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     7021 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     7081 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     7141 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     7201 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     7261 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     7321 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     7381 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     7441 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     7501 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     7561 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     7621 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     7681 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     7741 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     7801 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     7861 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     7921 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     7981 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     8041 gtttggcgaa ataaaaatac taaaaaa
//
LOCUS       DASHFF010000098         7756 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.099, whole genome shotgun sequence.
ACCESSION   DASHFF010000098 DASHFF010000000
VERSION     DASHFF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.099"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..124)
                     /locus_tag="U2I75_002605"
     CDS             complement(<1..124)
                     /locus_tag="U2I75_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1697663.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAF"
     gene            218..961
                     /locus_tag="U2I75_002606"
                     /pseudo
     CDS             218..961
                     /locus_tag="U2I75_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
     gene            973..1404
                     /locus_tag="U2I75_002607"
     CDS             973..1404
                     /locus_tag="U2I75_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1697664.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            1418..2863
                     /locus_tag="U2I75_002608"
     CDS             1418..2863
                     /locus_tag="U2I75_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HEN1697665.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            3149..4774
                     /locus_tag="U2I75_002609"
     CDS             3149..4774
                     /locus_tag="U2I75_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1697666.1"
                     /translation="MKKIYSKEIDCFRGLAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            5406..5759
                     /locus_tag="U2I75_002610"
     CDS             5406..5759
                     /locus_tag="U2I75_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697667.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
     gene            6540..>7756
                     /locus_tag="U2I75_002611"
     CDS             6540..>7756
                     /locus_tag="U2I75_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697668.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKKEKDFPIYNIYLHFYKASYNYHLIDFMYK
                     YPRSVLKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYY
                     FYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSF
                     RKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYE
                     NSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNP
                     DLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLDYYYVLDY
                     YDLFLTNVKPIKI"
BASE COUNT         2725 a         1044 c         1433 g         2554 t
ORIGIN      
        1 ggaaagccgt taactccgtt ttgagaagcg tattgacagc tttttccaaa tggacacgga
       61 ataattcatt caaatcgcct ttgttcgcta gagtctttag aatttctgta gtaaaatcgt
      121 tcatatggaa gtcctctttt ctgtgaatgt gttgtggtaa ctctattcta cagaagggac
      181 ttcctttttt ctatttacac aaaatatttt acactctcaa atttattctt tgctgtcaga
      241 aaaagaacaa gagaattttg atgaatatgc atattttgtg aatacggcaa tcgatcgaca
      301 atctttagga aaagttcttg gaggaaaaat gatctctgtt gtcgaacctt attatgaatg
      361 gataatggat cgacagcagt tagatccaac tacatctttt gatatgaaaa atgtaaattt
      421 ggttgatgat atggagcctt acattgagcg aaagctttat atcgttaatg ctgctcatgc
      481 ggcagtagct tatttaggcg cactgaaagg atataagacg attcaagaag caatccaaaa
      541 tatggagatt ttcaatctag tcaagcaatt tttaatggag aatatagagt actttgtaca
      601 aaaatatcat ttttctagag aagaattaac atcttttgtg gaaaaaacat taaaccgtca
      661 tgggaatcag aaactctccg atgaaattac tcgtgtagga agttccccta ttcgaaaatt
      721 aggccctaat gatcgtttaa ttgctcccat agtaaaacta gattcatatc atttacctta
      781 cgatgcagga ataaaagtaa ttgctgcggg atatcattat cggaatcttg ctgatccaga
      841 agctgaacaa ctacgtcgaa tgattgtaaa tgatgggttg aaagcaacga ttaaaaagat
      901 ttctcgtttg gaaggtaagc tgctgaatga agttgtcgct gcttatgagt cccagattta
      961 gaggaggaga atatgctaaa agaacaattg tcaggaaata caatgtttat ggatgcgatc
     1021 gattcatggc aagaggcaat ccgagtaagt gcgcgtcctt tgataggaaa gggaataata
     1081 gaggaaagct atgtacaagc gatgatacaa aatgtgttgg ataacggaaa ttatattata
     1141 ttactgccac tagtagcaat gcctcatgcg cgtccagaat ttgggagcaa aggggtcggg
     1201 atgagctttt tatgtttaga taagccagtc atgttcccaa gtgaagaacc agtaaaattc
     1261 ttctttacct tttcttctga ttcgccaaag ggacatttag acttaatggc caatcttgga
     1321 gaattgctga gtgatgaaaa aatgtatcaa aaattattcg atgtgaagac agaaaaagca
     1381 ttgttagatt tgataaagaa gtaaaggaga gaagaaaatg gaggtagcaa acaaaaaaaa
     1441 acgagtctcc atgcgagcta aagttcaagc gattggcggt ttcttaacaa atatggtatt
     1501 gccaaatata ggcgcattta ttgcatgggg aattgttact gcattattca ttccaactgg
     1561 gtggttccca aatgaatctc tgaatgaact cgtaagtccg acagtcagct atctattgcc
     1621 gttactactt gcctatactg gtggtcggat ggttggtggt acacgtggag ctgttttagg
     1681 tacaatcgga acaataggtt tgattattgg tgctgacata ccaatgttca tgggagcaat
     1741 gattatgggg ccacttggtg ggtggatgat gaaaaaaaca gatgattttt tagatgggaa
     1801 agttcctgct ggttttgaaa tggtcgtcaa taatttttct ataggaatta ttggtttcat
     1861 actgatgtgc ctttcttatc tggtagttgg accaataatc caatcagcta atcaatttgt
     1921 aacgagtgca attgaagcat tagtttcaac tggtttttta ccgatactgt ccttaatcaa
     1981 cgaaccggca aaagtcttat ttttaaataa tgttattgac caaggtgttt attatccatt
     2041 aggtatgcaa caaacattag aggcaggcag atcaatttat ttcatggtag catcaaatcc
     2101 tggccctggc ttaggactat tgttagctta ttctttattt ggtaaaaagg cagcaaaaca
     2161 gacagcacca ggtgcaatca tcatccattt tttcggcgga atacatgaga tttattttcc
     2221 atatgtatta atgaaaccac tcactatatt atctatgatt gctggtggaa tgacaggaat
     2281 catcactttt gaattatttg atgcgggcct ggtagcaggt ccaagtccag gttctatttt
     2341 ttcttattta gctctaacgc ctcggggaaa ttttttgggt gtcatggcgg gagtggtcac
     2401 agcaacactg gtttcctttt ttgtaacatc agtaatttta aaagcagata aatcaacaga
     2461 tgaagacaga aattttgaac tatcagttca aaaatcgaaa acgatgaaat cagaaggaag
     2521 aaaattatta aaagaagaaa atgctagaga aatatgtacc gagaaaaaag agatgaataa
     2581 aatttccttt gcttgtgatg caggaatggg aagcagcgca atgggcgcaa caatattcaa
     2641 gaaaaaattg caaaaagcag gtattactga tgttgagata aaaaattatc gaattgaaga
     2701 ggtaccagaa gatagtgatt tggtgatcgt acatcaagat ttggaggaac ggactagaaa
     2761 agcacatcct aacatgaaaa ttgtgacatt aaagaattat ttgcaagatc cagtaatcaa
     2821 tggattagca gaagaaatca ttggattaaa aacagaagac taatttatgg gaatttttga
     2881 ttaaccttta ttaaaattat tttgcctaat ataaaaaggt tgtgacatgg cttgtgtcgc
     2941 aacctttttt tgtcgcacaa ataattaatc ggatatttta tgttcatatt ttttgtgatc
     3001 aacgagaact gatggggcaa agatacattt tagatacttt taactgttaa tttacaaaac
     3061 aaggtgaaac gaatagaaaa atgaaaaaat cgatacggaa gaatatgttt gattaaaaca
     3121 tgcatgaaga gaaggaggtt gatgatacat gaaaaaaata tattcaaagg aaatcgattg
     3181 ttttagaggc cttgctgttt tagcagtgat attttatcat ttgccaaata gtttgctgcc
     3241 aggcggattg caaggagtaa cgatcttttt tgtggtttct ggttttttaa tggctaaaaa
     3301 tacgataaga gatgaaaaaa acagacaatt cagtttttta catttttact ataagcgtat
     3361 caaacgtatt tatccatttt tactgttttc tttggtcgta ttcattttca tactagggat
     3421 tagcaacatt cgattgatag gaaacattcg tagtgagcta cccagtttga tttttggcta
     3481 taacaattgg tggcagctga atcaaggagt atcttatttt gactcttact ttaatgcttc
     3541 tttatttaaa cattattggt cgctttcagt tgaactacaa ttctatttga tatggccatt
     3601 tttgtttatt gtcattaagc gtatgagaag aaaacaagtg ttttacttaa tctatacatt
     3661 gatttttgtt tctattcttt ttagtttgtt tttaccttca actaaggcat attatcatac
     3721 gatagctaaa ctttttccat ttttattagg agtttggggg tattttaatc gaataactat
     3781 tggccgtttc tttgaacaaa acacttttag taaaatctgg cttttattac tagctagtct
     3841 ttgtctgatt ctgttcccga tttttcctta tacactaaat gaactactga tcagtatttg
     3901 ttttgctcta ttgttagcca gtgtcgatga tatgaacatt gcaaaaaaga ccaaaatcgc
     3961 tagtacaaaa ctttcttttg taggaaaaat cagttatgaa ctttatttgg tgcatttccc
     4021 agtattgatt gctctgacaa atctattcaa acatcaccat tggaaatatt tggatatcgc
     4081 aggtctattc actgtgtttt tatgtacatt actcatgatt acttgcttta gaaaaggagt
     4141 gcaaaagatg agatcaccca aaatttggac agctatggca ctaatgataa tcggagtttc
     4201 tgtactggta ttacgtgctc ctgccagtcg actgaccgct gaccaaaatc agttgcaaag
     4261 tttattaaaa gaaaactcta gtaaagcaaa tgcaggagaa aatctgttgt cacaagagaa
     4321 gaaaatatta tttatcggtg attctgttat gctgggggcg tatcaggaac taaaggagac
     4381 atttaaaaaa gaagcattgg tagatgcaaa agaatcaaga caaatcactg cgttaccaga
     4441 aatcttaaag gagcataaag atttagtttc ttaccaacaa atagtgatcg ggttaggaac
     4501 caatggtgtg ataacagatg aagctataga acaaacaatg tctttgctga aagataaaaa
     4561 agtatactgg gtaaatataa aagcaccaac tggctggcaa gatacgataa atgccacatt
     4621 agctacattg ccaaatcagt attctaatat aacgatgatc gattggtata gtgagagcaa
     4681 aaatcatcct gaattttttt atgatgatga aacccatttg aatgaaagtg gacgtacagc
     4741 ttatgcaaag tatattgctt cagttattca ataaggtagt gattacgtgt gttaacctta
     4801 ctttttccta ataggtgaat caatttaaat aaaaaatact tcatatgata aacggtgttt
     4861 aagaggagga gctttttaat attcgtggta aaaaagagat gggcgaggtc gttaactgag
     4921 agcttcttgg aaagaaatcg ctttggattt atcctctttg ttatacagaa catgagcgag
     4981 acgacaatac taggatgtat gttctgatcg aagttttatg gttacaagca gcaatcagct
     5041 tagataaaat taaggatcgt aggaaccaag taaagaaggc tgttagtaaa gaaacaaacg
     5101 atcaagtatg ttttactctt aagaagatgc agtgaataaa taatagtaat ataatgctag
     5161 aagggactgt gacaaaagac gtgtcacagc cttttttgtt attttaatta tcgtccagtc
     5221 tttttttata aatgattgct taagcattaa atattaaaaa atatagagtt caaaattact
     5281 gcacaattag atgaaaaatt tgaacaacaa aaagtgtatt tttatactat aatagctttt
     5341 gaatttagtt actctattca aaagttatgc tatggacaag aaaaagaata aatagaggtg
     5401 agaaaatgaa tagtttcaag aaaaaatttg tgagtagatt tttaaatatg aaaaattttt
     5461 ttgaaaaaaa gggagattat gatgcatata attatgtatt aaatctagaa aagcaaacat
     5521 attatacact acgtaaaaac gatgataatg acttttggac tatgttacct aaaatgcttt
     5581 cgattgactc taagctttcc cttttagagg aatttattaa tttagacatt gaagaatttt
     5641 gcgggggaaa agagttaatt aatatggtag agagtgatta tcatacctat tataaagaaa
     5701 gttgtggata caaagtggat gagcaatgtc cacaatcaat tattttttac attgagtaat
     5761 atgaactatt aagcaggaaa gcaactagaa gctactgatt tagtatttac aaatataaac
     5821 tatattgcta acaattattt tctagaagga ataaaagaac taagaatcaa gacgttatct
     5881 tgattcttag ttcttttaac atggaaaaga tggataatgc actgttaatt catgatgcat
     5941 catcagaaaa gttggaagga gaagatagaa atcattaaat gatttctaca gatggtaaat
     6001 gaaaaaacaa aatattagca aaaatttgcc taaatagata gtaacacttt ctaaattaaa
     6061 tacaagcgca ttcataaaat gtttatacaa atatctatgt gataaaaatt ggatatgttc
     6121 acaataaata ggaattaaaa taacgaattt acgtttgttt ttatttacca atcaaattat
     6181 cttaatgcag gaataaaaaa ttaaattaca tattgttaat catttacatc tccattaata
     6241 actcaaaatg aaaagatgtt tttattttta ttctatattt cagaaaaaaa ttaaactaga
     6301 ttataataat tcttcatgta aaaataaaaa caattacagc tatgatgttt aagataaaac
     6361 aattatgaaa tcgatatccg tttatatact tatcataagc tagaagcaaa taactagcct
     6421 atgtatgtaa gtgagattat atttattaaa aggcttcttt tgttcgatca gttatagtcg
     6481 aacgagagaa gtttattctt tgattaaaat aaacaaaagt aacttattga ggtgaaatga
     6541 tgcaaacttt tttaactaaa acatctttca aaaaagtagt tttattagat tatttgttag
     6601 aaaaagacaa ttggtgtgca atggaagaac ttagaaattt acttaatgtt acggagaagt
     6661 cagtcttaca ctatatagaa gaactagagg atttatttaa gcaatataat ggtaatatcc
     6721 ttctgaaaaa tgaagataat aaacgatttt tcataaaaaa agagaaagat tttcctattt
     6781 ataatattta tcttcatttt tataaagctt cttataatta tcatttgatt gattttatgt
     6841 ataaatatcc tagaagtgtt ctaaaagatt ttgctaaaga gcaatttact agtgtatcta
     6901 ctgttttcag atatgcgaag ctcttaattc catattttag gcgttatcat ataacatttc
     6961 atccatttca gttagaattg aatgcatcag aggcaaacat tcgtagcttt ttttattatt
     7021 tctattggaa ttctacacgt gaaagtagtg ataagtggcc atttcatatc gaacaaaagg
     7081 aaatagagaa gtatatagtt gcttttgagg ggatttatga tattacgtta actatttttc
     7141 aaaaacgtgt attttctttt tggttagcca ttaatataga aagaagttca tttagaaaag
     7201 tacgagtaga taatgaatat aaatcagtta tctcagatga tccgcatttt aatcttttga
     7261 agaagtggtc aaaacaaatc aatctttctt ttaacagcga tgaactgtgt ttcctttatc
     7321 gaataattta ttcttttgga gtaattgatg gaaatgcaat atatgaaaat agtcatgcat
     7381 atgcacatca gcgacaaaat acttgttctt atcgagcagt tgagaatttg gagaaagttt
     7441 tacaaagcat gttccgtttt tctttagata taaaggatcc agaactcata ttcaatttta
     7501 ttgcatttca tgaaagaagt tatctatttt atggtaatcc agatcttttt tttaatcgtt
     7561 cgtatatcga agaaatgaaa gaagaagaac ctcgaactta tcacataatg gaaaaactca
     7621 aaaaagaatt acaagcaaat gcggatttgg atgtatcaaa gaaattagaa aactgggcac
     7681 aattattttt ggattattat tatgtgttag attattatga tttattttta acaaatgtta
     7741 aaccgattaa gatttt
//
LOCUS       DASHFF010000099         7696 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.100, whole genome shotgun sequence.
ACCESSION   DASHFF010000099 DASHFF010000000
VERSION     DASHFF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.100"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            544..1107
                     /locus_tag="U2I75_002612"
     CDS             544..1107
                     /locus_tag="U2I75_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1697669.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="U2I75_002613"
     CDS             complement(1386..1673)
                     /locus_tag="U2I75_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1697670.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="U2I75_002614"
     CDS             complement(1663..1992)
                     /locus_tag="U2I75_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1697671.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="U2I75_002615"
     CDS             2297..2626
                     /locus_tag="U2I75_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1697672.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="U2I75_002616"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="U2I75_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN1697673.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="U2I75_002617"
     CDS             complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="U2I75_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEN1697674.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="U2I75_002618"
     CDS             complement(4890..5498)
                     /locus_tag="U2I75_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN1697675.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="U2I75_002619"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="U2I75_002619"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN1697676.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="U2I75_002620"
     CDS             complement(6528..7496)
                     /locus_tag="U2I75_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN1697677.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2350 a         1532 c         1280 g         2534 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ttaggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tagttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaa
//
LOCUS       DASHFF010000100         7600 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.101, whole genome shotgun sequence.
ACCESSION   DASHFF010000100 DASHFF010000000
VERSION     DASHFF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.101"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(943..1356)
                     /locus_tag="U2I75_002621"
     CDS             complement(943..1356)
                     /locus_tag="U2I75_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN1697678.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="U2I75_002622"
     CDS             complement(1812..2297)
                     /locus_tag="U2I75_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697679.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="U2I75_002623"
     CDS             complement(2436..2651)
                     /locus_tag="U2I75_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEN1697680.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="U2I75_002624"
     CDS             2875..3675
                     /locus_tag="U2I75_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1697681.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="U2I75_002625"
     CDS             3694..5562
                     /locus_tag="U2I75_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1697682.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="U2I75_002626"
     CDS             5555..6046
                     /locus_tag="U2I75_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEN1697683.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="U2I75_002627"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="U2I75_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEN1697684.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="U2I75_002628"
     CDS             6606..>7600
                     /locus_tag="U2I75_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEN1697685.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DASHFF010000101         7395 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.103, whole genome shotgun sequence.
ACCESSION   DASHFF010000101 DASHFF010000000
VERSION     DASHFF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.103"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            67..150
                     /locus_tag="U2I75_002629"
     CDS             67..150
                     /locus_tag="U2I75_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN1697686.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            275..1012
                     /gene="erm(B)"
                     /locus_tag="U2I75_002630"
     CDS             275..1012
                     /gene="erm(B)"
                     /locus_tag="U2I75_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1697687.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1182..1442
                     /locus_tag="U2I75_002631"
                     /pseudo
     CDS             1182..1442
                     /locus_tag="U2I75_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1605..2270
                     /locus_tag="U2I75_002632"
                     /pseudo
     CDS             1605..2270
                     /locus_tag="U2I75_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2306..2551
                     /locus_tag="U2I75_002633"
     CDS             2306..2551
                     /locus_tag="U2I75_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1697688.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2654..3523
                     /locus_tag="U2I75_002634"
     CDS             2654..3523
                     /locus_tag="U2I75_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN1697689.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3504..4238
                     /locus_tag="U2I75_002635"
     CDS             3504..4238
                     /locus_tag="U2I75_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1697690.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4271..5179
                     /locus_tag="U2I75_002636"
     CDS             4271..5179
                     /locus_tag="U2I75_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN1697691.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5176..5718
                     /gene="sat4"
                     /locus_tag="U2I75_002637"
     CDS             5176..5718
                     /gene="sat4"
                     /locus_tag="U2I75_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEN1697692.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5811..6605
                     /locus_tag="U2I75_002638"
                     /note="aph(3')-IIIa"
     CDS             5811..6605
                     /locus_tag="U2I75_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEN1697693.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6881..>7395
                     /locus_tag="U2I75_002639"
     CDS             6881..>7395
                     /locus_tag="U2I75_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1697694.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2592 a         1117 c         1554 g         2132 t
ORIGIN      
        1 gtgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga
       61 ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt tttgaaacag
      121 actaaaaaca gtgattacgc agataaataa atacgttaga ttaattccta ccagtgacta
      181 atcttatgac tttttaaaca gataactaaa attacaaaca aatcgtttaa cttctgtatt
      241 tatttacaga tgtaataact tcaggagtga ttacatgaac aaaaatataa aatattctca
      301 aaacttttta acgagtgaaa aagtactcaa ccaaataata aaacaattga atttaaaaga
      361 aaccgatacc gtttacgaaa ttggaacagg taaagggcat ttaacgacga aactggctaa
      421 aataagtaaa caggtaacgt ctattgaatt agacagtcat ctattcaact tatcgtcaga
      481 aaaattaaaa ctgaacattc gtgtcacttt aattcaccaa gatattctac agtttcaatt
      541 ccctaacaaa cagaggtata aaattgttgg gagtattcct taccatttaa gcacacaaat
      601 tattaaaaaa gtggtttttg aaagccatgc gtctgacatc tatctgattg ttgaagaagg
      661 attctacaag cgtaccttgg atattcaccg aacactaggg ttgctcttgc acactcaagt
      721 ctcgattcag caattgctta agctgccagc ggaatgcttt catcctaaac caaaagtaaa
      781 cagtgtctta ataaaactta cccgccatac cacagatgtt ccagataaat attggaagct
      841 atatacgtac tttgtttcaa aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa
      901 tcagtttcat caagcaatga aacacgccaa agtaaacaat ttaagtaccg ttacttatga
      961 gcaagtattg tctattttta atagttatct attatttaac gggaggaaat aattctatga
     1021 gtcgcttttt taaatttgga aagttacacg ttactaaagg gaatggagat aaattattag
     1081 atatactact gacagcttcc aagaagctaa agaggtccct agcgcctacg gggaatttgt
     1141 atcgataagg ggtacaaatt cccactaagc gctcgggacc ccttgtagga aaatgtccta
     1201 agtgtggcaa caatattgta ttaaaaaaat cgttttatgg ttgttcaaat tatcctgaat
     1261 gtaagtttac tttagctgaa cattttagaa agaaaaaact aaccaaaacg aatgtaaaag
     1321 aattactgga gggaaaagaa accctggtaa aaggaatcaa aaacaaagag aaaaagccct
     1381 acaatgccgt tgtaaaaatt ggggaaaagg gatatattga ttttatctct ttttcaaaat
     1441 aaacatgggg ctgacccaaa agtagctcct atatgaaaaa tagaggtctt tccatttaga
     1501 tagtagtgga aagacctcta tttatctttt gaaagtgaat acaaaaataa agcacactct
     1561 gctataatat aggtgattaa tccatttgag agaggtgctt tatcatgtat caaaattata
     1621 ccataaacca actttgtttg cctatagatc ttgaaattaa attagaagaa aatgattttg
     1681 ctcatgccat tgtgcagttt gttgactcta ttcccgatga agtctttctt ccttattatc
     1741 aatctatggg tagaccccaa tatcatcctc gtatgctact ttccattatt ctttgtgcct
     1801 atattcaagg ggtttattcg ggccgccaaa tacaaaatat gctgatagat agtattcgga
     1861 tgcgctactt atctcaagag caattcccta actttcgtac tattaatcgt tttcgcgttc
     1921 atccgattat gaacgatatt ctagatcatt catttgttca atttcgtgag ctactggttc
     1981 aaagtggctt aatcagtggc agtgctctat acattgatgg tacaaaaatt gaagcggatg
     2041 cgaataaata ctctttcgtt tggaaaaaaa gtattttaaa gtataaggat agccttgatc
     2101 aaaaagccct tgaaaactac caacaaatgg tggaaacaaa aatcttacct gagttaatag
     2161 atgatttaaa ggatgaactt tctatagaag agattgaaag gattcgtcaa tctttggaag
     2221 aaaaggaaaa tcaattaatt gaagcgattg accaaaccga aacagtagaa gggggctgac
     2281 ccattattaa taaggagtgg acaacatgat tgatgtaaat gagctattgg aagaagctat
     2341 tagagaaact gaaaatctaa atgatggcga agtttttctt gttaaagatt tattcaaggg
     2401 atatgtgggg aataggatac ccagaaagga tcgacttctg cttggaacgt tatttttaaa
     2461 ccatgttagc aaaatgaatg gtaatttaaa ggctattgaa aagacttcat ctaatcagca
     2521 gaggtataaa aagacaattg ataagtttta actatctttt gtgaaattaa aaaaattata
     2581 aatatgttga gtagaagcaa aagatttggc ttggaatttt tacagcagtg aatcgataaa
     2641 aggagcatgc ttgatggtag ataatattat taaatcagta gcagagaaat tatcctctct
     2701 gtcttatata gaaggtattg ttttaggtgg ttcacgtgca aggggcaccc atacagagga
     2761 ttcggatata gatatcggca tctattacaa ttcagaatca tttgacataa atactattaa
     2821 tcaattcgct acaaagctgg atgatgagca tagaaataac cttgttgtac ctcccggagc
     2881 atggggtgat tggattaatg gcggcggatg gttagtcata aacgggtatc atgtggattt
     2941 aattttacgt gatattaaac gtgtggaaca aataatgaaa gatacagagc acggaattgt
     3001 tactgccaat tatcagactg ggcatcccca tggttatatt agtgcaatgt atcgaggaga
     3061 attagcgatt agcaaaatac tatatgctaa gaatgaaagc ttatgcgaat taaaaaaaca
     3121 ggcagaaact tatcccaatg ctttgcagaa aagtttagtt aactttttta tgtttgaagc
     3181 agggttctct ttaatgtttg taaaagcaaa ttcgggaaca gacgataaat attatattgc
     3241 gggtcatgtt tttcgtatag tttcatgttt aaatcaagtg ttatttgcat gtaataatgc
     3301 ttattgtatc aacgaaaaga aagctataaa actgcttgaa acttttgaac ataaacctga
     3361 aaaatatacc gagaaggtaa atcatatttt tgaagtactc ggtatctcac tttttgaatg
     3421 ctacgacatg accgagaagc tttataatga agtgaatgaa attgtatcgg agataaataa
     3481 ctttttaaac gaggagagtt cagatgaaag aaaacaaata tgatgataat atattttttc
     3541 aaaaatacag tcaaatgagt cgctcgcaga aaggactggc tggtgcggga gaatgggaga
     3601 ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt gcttgattta ggatgcggct
     3661 atggatggca ctgtatatat gcgatggaaa acggtgcttc ctctgtagta ggtgttgata
     3721 tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca ttttccacag attgaatatg
     3781 aatgctgtgc catagaagat gtggatttcc cagaggagag ctttgatgta atactaagtt
     3841 cgcttgcgtt tcattatgta gcagactatg agaatttaat aaaaaagata tataggatgc
     3901 tgaaggctgg tggcaattta gtttttacag ttgaacatcc tgtttttact gctcatggaa
     3961 cacaagactg gtattataac gaaaaaggag aaatactgca tttcccggtg gacaattatt
     4021 attatgaggg caaacggaca gctatgtttt tggaagaaaa ggttacaaaa tatcatagaa
     4081 cactgaccac atatctaaat acactgcttt caaatagttt tataataaat cagattgtgg
     4141 agccacagcc gccagagaac atgatggata ttccggggat ggcggatgaa atgcgacgcc
     4201 caatgatgct gattgtatcg gcaaaaaaga agatgtaata atatagaaaa aataaacgag
     4261 gagtatgtaa atgagatcag aaaaagaaat gatggattta gtactttctt tagcagaaca
     4321 ggatgaacgt attcgaattg tgacccttga ggggtcacgc gcaaatatta atatacctaa
     4381 agatgaattt caggattatg atattacata ttttgtaagt gatatagaac cgtttatatc
     4441 taatgatgac tggcttaatc aatttgggaa tataataatg atgcaaaagc cggaggatat
     4501 ggaattattc ccacctgaag aaaagggatt ttcctatctt atgctatttg atgattacaa
     4561 taaaattgat cttaccttat tgcccttgga agagttagat aattacctaa agggcgataa
     4621 attaataaag gttctaattg ataaagattg tagaattaaa agggacatag ttccgactga
     4681 tatagattat catgtaagaa agccaagcgc aagggagtat gatgattgct gcaatgaatt
     4741 ttggaatgta acaccttatg ttattaaagg attgtgccgt aaggaaattt tatttgctat
     4801 tgatcatttt aatcagattg ttcgccatga gctgctgaga atgatatcat ggaaggtcgg
     4861 catcgaaaca ggctttaaat taagtgtagg caagaactat aagtttattg aaaggtatat
     4921 atccgaggat ttgtgggaga aacttttgtc cacctaccgg atggattcct atgaaaacat
     4981 atgggaagca ttatttctat gccatcaatt gttcagggcg gtatccggtg aggtggcgga
     5041 aaggcttcat tatgcctatc cggagtatga taggaatata acaaaatata ccagggacat
     5101 gtataaaaaa tacactggta aaaccggctg cctggatagc acatatgccg ctgatataga
     5161 agagaggcgg gaacagtgat tacagaaatg aaagcagggc acctgaaaga tatcgataaa
     5221 cccagcgaac catttgaggt gataggtaag attataccga ggtatgaaaa cgagaattgg
     5281 acctttacag aattactcta tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat
     5341 gaagaggatg aggaggcaga ttgccttgaa tatattgaca atactgataa gataatatat
     5401 ctttactacc aagacgataa atgcgtcgga aaagttaaac tgcgaaaaaa ttggaaccgg
     5461 tacgcttata tagaagatat cgccgtatgt aaggatttca gggggcaagg cataggcagc
     5521 gcgcttatca atatatctat agaatgggca aagcataaaa acttgcatgg actaatgctt
     5581 gaaacccagg acaataacct tatagcttgt aaattctatc ataattgtgg tttcaaaatc
     5641 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     5701 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     5761 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     5821 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     5881 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     5941 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     6001 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     6061 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     6121 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     6181 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgatta gccgaattgg
     6241 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     6301 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     6361 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     6421 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     6481 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     6541 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     6601 tttagtacct agatttagat gtctaaaaag ctttaactac aagcttttta gacatctaat
     6661 cttttctgaa gtacatccgc aactgtccat actctgatgt tttatatctt ttctaaaagt
     6721 tcgctagata gagttctata ttagagatgt aaagagtttt ggtgaagagg tttgtgagta
     6781 tggcttttat aaccagggtg caatgagaag cactaacagc agctagctta agaacgcata
     6841 ggatatactt tttatggaag tcacagagga ggaataaaat ttgaacagga ttaacagagt
     6901 aacctctatt cttattcagc tgcaatcaaa aaaaataatt cctgccaaag aaattgcaca
     6961 gcgatttaat ataagcttaa ggacagttta cagagatatc cggacacttg aagaagccgg
     7021 aataccaatt ggatctgagg ccggaaaagg atattttctt gtagagggct tcctacttcc
     7081 gccggtaatg tttacagcgg cggaagtggg tgcattgatt acagcaggga aatttttaaa
     7141 ttgccatgga gatgaatcat ttataaagga ttttgattca gctatgtata aaatcaaatc
     7201 tattttgaag catggtgaaa aaaactatgc acaggagttg gagaacagta ttaacgtgta
     7261 cagcacatct ggacagaaaa atacattggc ggataacgtt attgcagcaa ttcagactgc
     7321 aatctgcaat aaaagggtaa tatcaattca atatcctgca tcaggagggc aagaaccgga
     7381 aagcagaatg ataga
//
LOCUS       DASHFF010000102         6197 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.104, whole genome shotgun sequence.
ACCESSION   DASHFF010000102 DASHFF010000000
VERSION     DASHFF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.104"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..122)
                     /locus_tag="U2I75_002640"
     CDS             complement(<1..122)
                     /locus_tag="U2I75_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEN1697695.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDR"
     gene            complement(706..1413)
                     /locus_tag="U2I75_002641"
     CDS             complement(706..1413)
                     /locus_tag="U2I75_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697696.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1478..2314)
                     /locus_tag="U2I75_002642"
     CDS             complement(1478..2314)
                     /locus_tag="U2I75_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1697697.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2332..3741)
                     /locus_tag="U2I75_002643"
     CDS             complement(2332..3741)
                     /locus_tag="U2I75_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1697698.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3755..5578)
                     /locus_tag="U2I75_002644"
     CDS             complement(3755..5578)
                     /locus_tag="U2I75_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1697699.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1925 a         1327 c          989 g         1956 t
ORIGIN      
        1 cgatcggata aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca
       61 tccattttac tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc
      121 aaaaagatac actcctatct cttatttaaa gttgctatat accctcttat tttagcatac
      181 ccccttttat aaaaagcaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
      241 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      301 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      361 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      421 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      481 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      541 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      601 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      661 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tctttttacc cattttcaag
      721 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      781 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      841 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      901 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      961 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
     1021 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
     1081 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
     1141 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1201 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1261 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1321 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1381 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1441 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1501 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1561 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1621 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1681 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1741 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1801 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1861 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1921 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1981 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     2041 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     2101 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     2161 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2221 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2281 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2341 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2401 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2461 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2521 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2581 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2641 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2701 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2761 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2821 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2881 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2941 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     3001 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     3061 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     3121 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3181 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3241 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3301 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3361 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3421 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3481 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3541 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3601 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3661 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3721 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3781 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3841 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3901 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3961 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     4021 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     4081 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     4141 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4201 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4261 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4321 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4381 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4441 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4501 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4561 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4621 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4681 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4741 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4801 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4861 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4921 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4981 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     5041 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     5101 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     5161 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5221 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5281 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5341 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5401 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5461 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5521 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5581 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5641 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5701 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5761 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5821 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5881 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5941 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     6001 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     6061 gtaagcaata gccaatttca atcattccga gatataaagg tatcaataga atgatttggg
     6121 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6181 agtaagtatt tcaattc
//
LOCUS       DASHFF010000103         6190 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.105, whole genome shotgun sequence.
ACCESSION   DASHFF010000103 DASHFF010000000
VERSION     DASHFF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.105"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(1..3986)
                     /locus_tag="U2I75_002645"
                     /pseudo
     CDS             complement(1..3986)
                     /locus_tag="U2I75_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; internal stop; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            complement(4603..5037)
                     /locus_tag="U2I75_002646"
     CDS             complement(4603..5037)
                     /locus_tag="U2I75_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697700.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(5054..>6190)
                     /locus_tag="U2I75_002647"
     CDS             complement(5054..>6190)
                     /locus_tag="U2I75_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN1697701.1"
                     /translation="NSEFSIIKEYRSDRTTYFFYDYKKLLTDNIKKTKDFHFFTGDFN
                     EMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNLNVAMADRERLYNSLQNLTLYSLG
                     DIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLY
                     ENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLG
                     MDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQ
                     FIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNH
                     TISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT         1744 a         1214 c          909 g         2323 t
ORIGIN      
        1 actggaactt tcactgtatc tttcgtacca tctggatacg ttacttcgat aaccccttcg
       61 taattacctg gtgtgttggt atcaatcgta ccaccaggag tgacatctgt tactgttgtt
      121 ccttctggta atgttggtag gttggttaca ttatctgtta gatctactgt gcccccaact
      181 tctacttttt cgccttctac tgttggttca tatttatcgg catcggaacg attgtctgtc
      241 acttctactg gaactttcac tgtatctttc gtaccatctg gatacgttac ttcgataacc
      301 ccttcgtaat tacctggtgt gttggtatca atcgtaccac caggagtgac atctgttact
      361 gttgttcctt ctggtaatgt tggtaggttg gttacattat ctgttagatc tactgtgcct
      421 ccaacttcta ctttttcgcc ttctactgtt ggctcatatt tatcggcgtc agaacgattg
      481 tctgtcactg taacatctac ttcaacttca tcagatgatc catctggata tgtcactaca
      541 acagtccctt ttttatttcc agctgcgcta acatctactt tttctttcca agtatactta
      601 gtatcttttg gtaaatcttc tttgttctta attccctcaa atgcgtctgg ttccttattc
      661 aattctgtag tcactttttg accttcagga ttatatttgt cagcgtcaga tttatcatcc
      721 agttttgttg cacttgatac aaattctgta tcatctgctg taattggttg agttgcttcc
      781 tctggccatt tcgcatctgt ataaccatct tttgctttca acactggaag tttatcttct
      841 ggtaagctta cgccgtcttt cactgcaaag actgttgttc cactttcaat acttgttcct
      901 tctcctgcgg tgaaggttac tttgtggtag cctgcaggaa tgttttctcc tggattttca
      961 atgatatcat ccagttttgt tgcacttgat acaaattctg tatcatctgc tgtaattggt
     1021 tgagttgctt cttctagcca tttcgcatct gtataaccat cttttgcttt caacactgga
     1081 agtttatctt ctggtaagct tacgccgtct ttcactgcaa agactgttgt tccactttca
     1141 atacttgttc cttctcctgc agtaaaggtt actttatgat agccagccgg aatgttatcc
     1201 cctgggtttt caatgatatc atccaatttt gttgcacttg atacaaattc tgtatcatct
     1261 gctgtaattg gttgagttgc ttcttctggc catttcgcat ctgtataacc atcttttgct
     1321 ttcaacactg gaagtttatc ttctggcaag cttacgccgt ctttcactgc aaagactgtt
     1381 gttccacttt caatacttgt tccttctcct gcggtgaagg ttactttgtg gtagcctgca
     1441 ggaatgtttt ctcctggatt ttcaatgata tcatccagtt ttgttgcact tgatacaaat
     1501 tctgtatcat ctgctgtaat tggttgagtt gcttcttctg gccatttcgc atctgtataa
     1561 ccatcttttg ctttcaacac tggaagttta tcttctggca agcttacgcc gtctttcact
     1621 gcaaagactg ttgttccact ttcaatactt gttccttccc ctgcggtgaa ggttactttg
     1681 tggtagcctg caggaatgtt ttctcctgga ttttcataaa tatctttact tacagttact
     1741 gctaaatcgg tcgtgcctac agaaccatct tggtaaacaa ttttaatagg aattgttcca
     1801 gtaatcggct ccgattctcc tacaccttta gctggagtga atgtaacaat accagtgttt
     1861 gcatcaacac ttgcattacc gaagtcctta ttatagacat ctgaaattga aaaggcatct
     1921 ttagctaaag gaacatctgg tttatcaaat gtaggttttc cactttgaac tgtcgtttct
     1981 ggagttgttg ttataggtac gtatgttgca tcattttcca tagcttgaga agcttttaca
     2041 gcaaactcta catccacgtc gaaagttcga tttccgatct taacagttac aatacctttc
     2101 gtactatcag taacgttaga agtatcaggc tctgttttcc atgaatactc tgcatcttca
     2161 ggtaagtttt tattattccg aattgattct ttagcatctg gtttaaatcc aactaccacg
     2221 gtttgtttat caaccgcttt tggtgattcc ttaataacgg ttgaaccttc ttctggttta
     2281 tcaaaacctg gagaaacgat ttggattttt aattcatctt tcgcgattaa tttacttgaa
     2341 tctacacccg taaattcaaa ttctacagta tctgaaattg gagccccatc tttttcattt
     2401 ggagcgatag ttttttctgc taatacagta ccttctttat caacaagttg ggctttgtga
     2461 cctgttcccg ctaactcgtg gatgaatact ttccccttaa ctgttgtgtc aacatcatat
     2521 acttcttcta attcaggttt cccgatatat ggattcgtgt ctccgctctc ttctttatcg
     2581 tcagggtttc catcaccatc aatatcttgc aaataataat aacctgttcc ataagtgttc
     2641 tggattaatt ttttattcgt atccgacaac caagaatcaa agatgaaatc ttcaccaaat
     2701 ggtttatttt cttcaacttg tgctgttgct ttcatatctt ctaagatttc atctaatggc
     2761 ttagtcaatt gtaaaacata tcgaataacg actggacgtg gacttgcatt agcaaaatcg
     2821 ataagtttag ttaaatcatt attagttatt gaacctatgc cataattttc atgaccttcc
     2881 cgtcgctcga ttgctaaatc agctactttt gttccggatt caaacgaaac atcatatgtt
     2941 actttgtcag cctgaaccat gtgcatctgt atacccgcta catatggaac taatcgttca
     3001 tcaattttgt aattagcata ccaaggcgtg ccctttgcgg aagatgaata tgagaaatta
     3061 taatttttcg ttaacttatg atctaagatt atagctccat tagcacctgt ggatcctgaa
     3121 ccatcttttg tttgagtatt aggtttctca tattttgcac ttactgatcc atgtttaaac
     3181 tggtcaggat tattttcaga tacatctaag ctattataga aatcatctga atctgttaaa
     3241 aagtagcccg tactatcaat acccggtaca attttatcat cgtcttgatg acttaccaag
     3301 tagattcgat acattaaacg atcggtcttg agattgtcta tgcctatctc tttaaaaatt
     3361 tcatctaggc tcttttcaaa cgtaattacc ccattaggag cggtttgcgt atcggttgtt
     3421 tctgccccag caaataatcc atcacttgca cgaatataat tgaccttcca aatatttgta
     3481 ggtctaccca gcgaatcatt aattcttaca aaggtccgtc tagaagatcc aattcctttc
     3541 tttggatcga cctgaatttt ttctacatat ttagctaatc gttcatctaa ttgaagacgg
     3601 atttcccagt ttttagttga tgctgcaata ttatgttttc cgtaaacttc aaatccaatt
     3661 gtattgcctt ttagtgtgtc tccagttagt tcttgaggac taaattgtaa atcctaaaag
     3721 ttataatttt tcgtataacc aggattctcc tgaaaagtat tccccgaagc tactttgtca
     3781 cctacttgta cggagcttat cgctgtttca agtgggctgc ttggagaaat ttcatcttgt
     3841 gctttaacta gttcagttgt tcccaagaca agaccaacac caatcaaaac agatgcaacc
     3901 ccaacactta attttttgat actgtacttc aatactcgtt tctttgtttt ttctagaaat
     3961 actctcttat tattcttgct aaccattatt caaatcaact cccttaataa ttactttcct
     4021 agtaagaata aaagataaat ctgatacgta ttattcacag aataggaatc ttactaacct
     4081 tatgttaact aatttactta gagaatttca tgaacattac aacaaaaaaa gtacatttct
     4141 tcatgttttt ttcggatatt tgtgcttttt aaccattcag cagctgaaaa catataaaaa
     4201 gatatgtatg tattctttct gaaattaagt tcatttctat gcgatgaagc atgttttcaa
     4261 aatattaccc tttcttagat taaacttcag agtattcaaa taacggaata aggagcctta
     4321 tcccgcatta ctgaatgaga ctgtgacata agtaaaaagg tgacttcaaa ttccttcttc
     4381 ggaatttgaa atcaccttct ttataattat aaagtatcgg tttcacagca tttctgaatg
     4441 attcctagaa tcttaaacga atgattggag ttatcaacat tttttcactc gtttttagcg
     4501 aaacaaaaaa tgacatttgt cacagcctca ttaaattatt gattcttttt ctttataatt
     4561 gaattgtcca tatttaaagt gtctagtact ctacatgaat aattaattgc tttcactcaa
     4621 atacttataa tctattaact tgtagttgtt taacttttct gaaaaagtta gacccacgta
     4681 ataatcttcc cagctttctc tttcaattaa ctgatccaat ttttgataat ccataaattc
     4741 atcatgtatc aaaaagaata aataacttga tattttagca tctagcttaa tcagattaaa
     4801 taatgtttgt ttaaaattgc taccattaat atgactgata gattctctag cttcttcaca
     4861 taaacttttt actttattaa ttactacttt gtcatattca tttaacacac tattattacc
     4921 attacttaga cgctcagatt ctacgaaaca actcaaatca gaaatttccc aaaaaaattt
     4981 tttgttaaaa tccaatttcc tcaactcctc caaactacgt aaatgatata gtctcataat
     5041 aaaataaaac ctctcagcta atgttgttga aattgtccac taaacctaat tgatattgct
     5101 taggagatac acctgcgtac tttttgaata ctctcgaaaa gtgtgaagaa tctgaaaatc
     5161 ctaaacctaa agaaacttca ctgatagtat ggtttgattt caacattatt ttagccgtag
     5221 aaatttttcg cttagagata taattataaa tcgtaatatg catttcattc ttaaagcttt
     5281 ctcttaattt agtttcactt acagaaaaat aattagctat ttcctttaca gtaattcttt
     5341 tatagatatt taagccgatg aattgaacga cagaagaaat taagggagaa agatgctcta
     5401 tcttagtttt tccgatttcc ttagtataat aaacaatccc agaatccctc acttttaaga
     5461 cctcaggtaa cgatacagct aattcgtttt cttgtatcaa agcatctcta ctttgatacg
     5521 cttctattat atccattcct aatgttatag acgtttgtgc taacttttca aaaacaatga
     5581 tagaataatt tttttcagac cgaatcgtat ctccactaac acttggaatt attccattac
     5641 tcagattaaa aaccatgttt tctaaatttc gaatatctcc atttttaaca tattccagta
     5701 ttttattttc ataaagaaac agatatattt ctggatcgta attttgtgat aacagattat
     5761 ctattttgat ttgctcgata gttttactta gattttctgt atattcaatt aacggttcat
     5821 ggaatagatc ttctattttt ccagtaaaaa aataatgaac taaaatcaga atatctcgaa
     5881 tgtctcctaa agaatacaaa gtcaaatttt gcaatgaatt atagagtcgt tctctatcag
     5941 ccattgcaac attcaaattg ttcattttta gtttaaaaaa aactttatca atattatttg
     6001 ctctaaatgg accaaataag taatatctgg ttttaaacat gtatagaaga aacatctcat
     6061 taaaatcacc agtaaagaaa tggaaatctt tagttttttt aatattatca gttagtaact
     6121 ttttataatc ataaaaaaaa taagtagttc tatctgagcg atactctttt ataatactga
     6181 attcggaatt
//
LOCUS       DASHFF010000104         6128 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.106, whole genome shotgun sequence.
ACCESSION   DASHFF010000104 DASHFF010000000
VERSION     DASHFF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.106"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            196..921
                     /locus_tag="U2I75_002648"
     CDS             196..921
                     /locus_tag="U2I75_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697702.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="U2I75_002649"
     CDS             911..1420
                     /locus_tag="U2I75_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697703.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="U2I75_002650"
     CDS             complement(1490..2938)
                     /locus_tag="U2I75_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1697704.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="U2I75_002651"
     CDS             complement(2938..3654)
                     /locus_tag="U2I75_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1697705.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLAAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="U2I75_002652"
     CDS             complement(3635..3985)
                     /locus_tag="U2I75_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN1697706.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="U2I75_002653"
     CDS             4129..4902
                     /locus_tag="U2I75_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1697707.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="U2I75_002654"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="U2I75_002654"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5744..>6128)
                     /locus_tag="U2I75_002655"
     CDS             complement(5744..>6128)
                     /locus_tag="U2I75_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1697708.1"
                     /translation="LKPEGRKAVDIENSINEWLQSVLKNLFKSITFDCGKEFSNWKSI
                     SNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMDFHQVNQGFISSVASKRNHI
                     PRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         1775 a         1189 c         1071 g         2093 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 agcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt ttttttaatt attatttgtc aaattaagcg agacaaaact
     5761 ttccatttac gtaactcaaa aaaacttcta atggtgtttg gtaatttagt gattttctag
     5821 ggatatggtt tcttttggac gcaacggatg agatgaatcc ttgattgact tggtggaagt
     5881 ccatttcttt tggtagtcca tcttttcgga ggagtccgtt tgaatgttca tttaatcccc
     5941 gttgggaagg cgttcctggg tctgcgaaat aaatatcaat atcattcgta ttactaatac
     6001 ttttccaatt agagaattct tttccacaat caaaggtaat tgatttgaaa agatttttga
     6061 gtacggattg aagccattca ttaatcgaat tttcaatatc aactgcctta cggccttctg
     6121 gtttcaag
//
LOCUS       DASHFF010000105         6084 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.107, whole genome shotgun sequence.
ACCESSION   DASHFF010000105 DASHFF010000000
VERSION     DASHFF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.107"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..676
                     /locus_tag="U2I75_002656"
     CDS             <1..676
                     /locus_tag="U2I75_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1697709.1"
                     /translation="KKIAPDHQIAIYTHTDKDHIHNHIVINSVNLETGKKFQAHGQAF
                     LNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEKNEPVWKDEIRIAIDQTMKN
                     KKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRSNRLGKDYEKETILHELDR
                     RERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEIRPARRNHEQHVERGTDH
                     QQELSL"
     gene            916..1605
                     /locus_tag="U2I75_002657"
     CDS             916..1605
                     /locus_tag="U2I75_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697710.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLI
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKIEEFTA
                     CYEDMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1726..1812)
                     /locus_tag="U2I75_002658"
     CDS             complement(1726..1812)
                     /locus_tag="U2I75_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1697711.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            2449..3393
                     /locus_tag="U2I75_002659"
     CDS             2449..3393
                     /locus_tag="U2I75_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEN1697712.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3414..3947
                     /locus_tag="U2I75_002660"
     CDS             3414..3947
                     /locus_tag="U2I75_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1697713.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            3962..4132
                     /locus_tag="U2I75_002661"
     CDS             3962..4132
                     /locus_tag="U2I75_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697714.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            4512..4736
                     /locus_tag="U2I75_002662"
     CDS             4512..4736
                     /locus_tag="U2I75_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN1697715.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            4756..5043
                     /locus_tag="U2I75_002663"
     CDS             4756..5043
                     /locus_tag="U2I75_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1697716.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            5484..5864
                     /locus_tag="U2I75_002664"
     CDS             5484..5864
                     /locus_tag="U2I75_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN1697717.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            5846..>6084
                     /locus_tag="U2I75_002665"
     CDS             5846..>6084
                     /locus_tag="U2I75_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1697718.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYA"
BASE COUNT         2301 a         1027 c         1132 g         1624 t
ORIGIN      
        1 aaaaaaaata gcccccgatc accaaatagc tatttacaca catacagaca aagaccatat
       61 tcataatcac attgtcatta actccgttaa tctggagact ggaaaaaaat ttcaagctca
      121 tggtcaagca tttctaaaca aatgttatga cctaaatgac gagatttgtc ttgcacataa
      181 cttaccaatc actgagcgag gaaagaaacc agaaaaacga accatgtctg aaataaaatt
      241 aaaagaaaaa aatgaacctg tttggaaaga cgaaattcgg attgcgatcg atcaaacaat
      301 gaaaaacaaa aaaaccagaa cctatgacca attttgtaat tccctcaaaa cattggggat
      361 tcattgcttc aatcgcggga aaaatttcac ttacgaattg atccacaaaa agaaaaaagt
      421 acgatccaac agactaggaa aggactacga aaaggagacg attttacatg agctggacag
      481 acgagaacga acctataacc aacagcgaac caacgagcta cgaagagctc gagctagcat
      541 tcgcgaaact gcagaagaag tacgaagaga acaacgaact cttacgcctg aacaaccgga
      601 aattagaccg gctcgaagaa accatgaaca acacgttgaa agaggaacag accaccaaca
      661 agaattatcg ctctaaaacg ttaaaagaca tagaaaacga aataaagaaa caacgtgctt
      721 ttcttgctcc ctacacacaa gaaaggatac aaactacgat tgatttacaa gttaaaaaac
      781 tccatcaaga gctattagat cagcgaaata aagaacaaaa aaaggcgttc attcaacgat
      841 caatggattt tcttgccccc ttatgttgct tagtcatgac actgttcgca ttttggttag
      901 tatggggagg ctaaaatggc attttattta ccctatctac tcatttttgt tagtatttca
      961 ggaagtattt ggctcatcta taaaattttt caaacaagat attcattaaa aggctctaaa
     1021 attcgattta agcgattttt cttgattggc tgtatatttt ccttgataat cgtttcaagc
     1081 ggcttattcg gcgttttaga gggcaataag cgcgtttcta gaagtatttt attaggaaat
     1141 gtcactcaaa agtatgaatc agcacgtgat aaaaagaaaa aagagcaagc attagcacaa
     1201 aaaattgaag aatttaccgc ttgttatgaa gacatgaacg atatttttgt gaatcaagaa
     1261 aaacgtttga cggataaaaa catggaaaaa ctcactcgtc tttatcaaaa cttgccagaa
     1321 aaacagcaaa aagaggttca agacaactat gagcgaacca aaaaagacgt gcaatatttt
     1381 aaagatacgc agacagaaga agcttgttat aatttattca gtgatacaat ccctttttca
     1441 actagtgaac aggaacgaaa agaacggcaa caatcagtta cctacgaacg ttataaagca
     1501 ctgcttcagc aagcaacgaa catacaaaat ccaacgaaaa aagaaacggc attaaattat
     1561 ctaaaaagtg tcaaggaatg gttagatcag caacagcaaa attgaatttg atcggcaatc
     1621 atgttaaact aaacatgcaa atgatcaaac attagataca cgaaagcccc agagaggtcg
     1681 gattctctgg ggcttttttt caggttgatg tcgcctttat tgattttact tcttgtttcg
     1741 gttattcaac caataactga ataaagagac acaaacttct ataagaaggg ggcaaatgaa
     1801 caattcaaac attagataca cctccttcca gtcgcaagac tgtcggatag gcgacaagtt
     1861 attatatctt aaaaaattaa acttgtgtgg tgtttttaag tatcgcttat tttagggttg
     1921 ttagggcttg ccctgaccgc ctgtaaggcg ctttaaagct agataaggat ttttatccaa
     1981 gactgtctta aaacggctta gataagcatt cagaaccgct caaaggcctc tttaccaaca
     2041 tgtttttagc aaaggagcct ttaggcgact gcgcagtaca atgtgagagc gtagcgatca
     2101 cacctttttg gcgaccgtag gaagcaaggt tttggattgg cagttttgaa aaatgcgcga
     2161 acctgtttaa agaaaaaaga agtggttttt aggcgggaaa attagccccc ctctttttat
     2221 atctcttttt aaacctcttt ttaaacctct ttaccgtaag ctgtgtcctt actctaccaa
     2281 tggatacaga atgttatgaa ggacattttg tctgtttatg aaggacattt tgtctgttta
     2341 tgaaggacat tttgtctgtt tatgaaggac attttgtctg tttgaacgtc aaatatgaat
     2401 gtcttgacat ttaaaaaatg tcattcataa ttggattgag gtgattatat ggctaatgag
     2461 attgtaaaat accaccatga attaaataca attcctttaa gaaaatttac accggttgaa
     2521 atgaatttgt ttttttccat cgtgtctaga atgcgtgaaa aaggggataa gacagttcgt
     2581 tttacgtttg accaactaaa agatttaagt aactacaaag caactgcaaa tgtacgattt
     2641 gtagaagatt taaaagcaac ttatgaaaaa atcttatctt tacgctttgg tcgtagaagt
     2701 gagtctgggc tttcttacga tatgtttgta atgtttacag aatttaaaat caacggggac
     2761 gtagaaaatc cttacgtaga tatacgaatt tacgaaaaag cactgccttt gttaaataat
     2821 ttagacgagt gggtacgcta ttctttacaa caattcaacg agctacaaag tggctatgca
     2881 aagactatgt ttcgtctgtt aaagcaatac agaaccaaag gtttcgcata tttctccaaa
     2941 gaagactttc ttgaattatt agatattcct aaaagttata agcaacctga tatagataaa
     3001 cgagttataa aacctatacg tcaagaacta actgcaattt tcaaaggact aacaatcaaa
     3061 aagaaatacg gaaaaggacg gggaaaacct gttattggct atcaatttac gttcaaacct
     3121 gagatcaaaa aagcagacga ctttaacaaa catgcaccaa ccaaagaaga ccaaaataaa
     3181 gcattggctt tagctgataa acaaaaagaa tacgaagaac ccgctacaac aattaacgaa
     3241 aaaatcaaac agaagaaacg agaacttgaa ctcctccaac aaatcaaaca actagaaaaa
     3301 gagaaaaaag ctcttgaaaa acaaacagag tccgcacttt cggaaaaaga gaaaaaaagt
     3361 ttattatctg atttagataa tttattccag tagcaaccta ggaggcaacc agtatgcaac
     3421 cacaagaaac ctttacaata aaggaattgt cagatctttt taagatgagt aggcaagcaa
     3481 tatcgaaaca tatccaaaaa ttagattctt ccatgatagc taaaaatgag cgtggttata
     3541 aggttgtatt gcgttctgga gtattgcaac ttgctcgcaa cttagacaat caaaggttgc
     3601 tagataagtt gcataaacat gaacaaaaca acgataaaaa tgcttatata ccaaacaaaa
     3661 acgtggttgc aaacaagttg ctagatcagt tgcgtgaaga aaatgaattt ttacgagaac
     3721 aacttgccct caaagagcaa aataattctc atatacaaaa actactcgac cagcaacaac
     3781 aactcaccct gcaagccaac caacaaatcg aaaaacttca ggagcaactg cagttgactt
     3841 atacggaaga gtcttcagag aatagttcaa cgactctttc tgaagcaaca gaaacaatgg
     3901 acgaacaaac aaagaattgg aaaaaatggt ggcagctatg gaaatgaaga aggaaatact
     3961 tatgacaaaa aaacacttgc tattcggttc tatcctacta ggaatattaa gctttagccc
     4021 aatgttatta tcagtggcca acaaaggaaa taaaaaaggg gaactaaaac tcaatcctaa
     4081 ttactacaac agtcaaaatg aggaagtatt tgtagaaaaa cttgataaat aagaaaagac
     4141 tgctcgtacg agcagtcttt ctgaaagaga ttatcctatt attaacctta gaaaagaata
     4201 ataaatgaat atgagatatg taaaaagcat aacaaacaca aacaaaaata acaagtattt
     4261 aaaaaatgac cgatcaatat tcacaaaatc agcgcctgga tcccttggta caggcgttac
     4321 ttggtagtat gtacattatg tctactagct gtttaaataa ctgatcttat aattttattt
     4381 aaacgattat gaattcaaaa ctacttttta tgacgttaat cattgttata taataaaatg
     4441 ttatataata aaattactaa gattatttaa tataacaagc aattttaaaa aaacgaaaag
     4501 gaaaaacaat catgaaaaag aaagtattaa aacattgtgt tattctagga atattaggaa
     4561 cttgtctagc tggcatcggt acaggaataa aagttgatgc agctacttac tatggaaatg
     4621 gtctttattg taacaaagaa aaatgttggg tagattggaa tcaagctaaa ggagaaattg
     4681 gaaaaattat tgttaatggt tgggttaatc atggtccatg ggcacctaga aggtagtggt
     4741 agggaggtta ttataatgga ttttactaaa gaagaaaaac ttttaaatgc aattagtaaa
     4801 gtatacaatg aagcaactat agatgactat cctgacttaa aagaaaagct ctttctttat
     4861 tctaaagaaa tcagtgaggg aaaaagtgtt ggtgaagtta gtatgaaatt aagtagtttt
     4921 cttggaagat atattttaaa acataaattt ggattaccta aatctttaat agaattacaa
     4981 gaaattgtta gtaaggaatc tcaagtatat agaggatggg cttctattgg tatttggagt
     5041 taattctttg gaataaaatg aaaatgggac tgaagaatca attctgattc atcagtccca
     5101 taaattatag agccgaaaaa ttatcatatt tatagattta ttaatagtaa atggcataag
     5161 aatctttgaa caacgaaaaa aatgtagctc tacctaatta ttgatttgca acaatttcac
     5221 cgaaggtgaa acaagcgaaa ctttacaaaa agtggctagc atttgctagt cttttttgta
     5281 ttgtggagag tgggggggag ggggattcaa gggggagctt gcgccccttt gacaagcggt
     5341 tatttgcacc aaatacgtag gtatttggct agcaaatata gagcttgcca aaccctttta
     5401 gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat acgtccgtag
     5461 gacgtactga aagggtggga cttatgagcg aacaaaatca aaatttggct agcgatcatt
     5521 ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg agcgaatctg
     5581 aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg gctttcgtaa
     5641 aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac attgcccagg
     5701 caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgct aagtggtgta
     5761 atacacacca acacgacgtt gctcctggag acgcccaacg cttatccgaa aacctaaaaa
     5821 tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagccgaa ccacttcttg
     5881 tagtcgtgca attaattacg ctgaaccacg agcaaccatt aaaacaggaa taaactgtga
     5941 cgtcgattat gccaaaagtg aaatgaaaca aattcgcatg ctgtatggaa aagacgaccg
     6001 tgtacaggca cacttattga ttcaatcttt tcgaccagga gaaattaccg ctgaaaaagc
     6061 taatcaacta ggggcagagt atgc
//
LOCUS       DASHFF010000106         6079 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.108, whole genome shotgun sequence.
ACCESSION   DASHFF010000106 DASHFF010000000
VERSION     DASHFF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.108"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..411
                     /locus_tag="U2I75_002666"
     CDS             <1..411
                     /locus_tag="U2I75_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697719.1"
                     /translation="LIGGYFLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDE
                     ELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVN
                     TDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            425..1114
                     /locus_tag="U2I75_002667"
     CDS             425..1114
                     /locus_tag="U2I75_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697720.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1239..3590
                     /locus_tag="U2I75_002668"
     CDS             1239..3590
                     /locus_tag="U2I75_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN1697721.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3670..4065
                     /locus_tag="U2I75_002669"
     CDS             3670..4065
                     /locus_tag="U2I75_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697722.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4390..5007
                     /locus_tag="U2I75_002670"
     CDS             4390..5007
                     /locus_tag="U2I75_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1697723.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5048..5347
                     /locus_tag="U2I75_002671"
     CDS             5048..5347
                     /locus_tag="U2I75_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEN1697724.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5381..5548
                     /locus_tag="U2I75_002672"
     CDS             5381..5548
                     /locus_tag="U2I75_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1697725.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5593..6063)
                     /locus_tag="U2I75_002673"
     CDS             complement(5593..6063)
                     /locus_tag="U2I75_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1697726.1"
                     /translation="MEFREGAFDFQTSQQNPSLDRLGRDYEDIKNTVAFMKQKKVALK
                     ILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKG
                     RPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2085 a          843 c         1226 g         1925 t
ORIGIN      
        1 ttaattgggg gctattttct tttgaattta gattctgtta attcaaacgc tcaaataaaa
       61 gatcaaagag tagctgaaca taataataaa aaaatgagtt tgaccaaaaa tatctattct
      121 atgtcagatg aagaattgta taaagaattt ggtgatttat ccggggaaca gttagaagaa
      181 agtattccag gaattagtga ctcagataaa attgttgtct tgtctgctgg tgggcaatta
      241 atgggagatg gcaaatccga attagttagt tataaacaaa atcacagaca gtttattgat
      301 gttaatactg attccaacgc tatttcgatt aaggatctaa aagagctttt aaatagtaaa
      361 agaaatgaat taaagcagat aaaagaaact aattcagtgg aattagagta aaggaagatt
      421 cattatgaaa aagatttttg gtgtttctgt tgggctactt atgatgttaa catttggaaa
      481 tgcagtatat gctgctgaga aagaagttat ttcagatcca gagcagtacg cctcacgata
      541 ctttgctgat tttgaagaag aagataaagt acttgcattg ccagaaggtg gttttttaaa
      601 aggggaagct aaaattgtat ctatggataa tccagatata attcaaggtt catataattc
      661 agaaactgat cctgcttcag tttcagtgac agaagcaaaa aaaattgttg ctgaaaaatt
      721 caaaaatgat attgaaacta ctccaattat gcctcgagga gcatttccac ccgatgttgg
      781 tactagcgga caccttcttg tactagcagg aggaggaagc tataaatcac agccatttag
      841 tggtgcggga tggcgttttg gaggtatggg tgtaaaacca gctgatggta caggcaactt
      901 tttacgttgg aaatcctaca ttgacgatgg aagagtagga aatgtaaatc aagccaacgc
      961 aactaaatct ggttctattc aaggaatggc attgtatgct cgtgaaggat tcaaatactt
     1021 tgcaccaaac gggcctggtt ggacaatgaa tataggtatt tattacactt ataatcctgt
     1081 tgcaggtacg tattatgttg ttcaaaatat ttaataaaac agcacaaaga gatttctttg
     1141 tgctgtttta ttatttttag ttgttgacaa acactaaaat ataaaatata atcaacacat
     1201 aaacgaacgt atgttcggca agaaagcagg ggatttttat ggatgagatg gttttaagta
     1261 ctcagaaatg gctaaacaag aaatacagta atgttacagg gtttgataag gttccagaaa
     1321 atgggcgaac agggtggcct acaatctatg gacttattga aggactacaa gtagaacttg
     1381 gtattactaa tctagtagct aattttggac ctaccactga aaaaatgtac gataatcagg
     1441 ttactcctaa atggggcaaa aatctaccta aaaatattgt atttcttatt caaggtgcct
     1501 tttggtgtaa ggggattaat ccaggtggct tcgatggggt atatactcca tatttagata
     1561 ctgctgtaaa agagttgcag actgatgcag ggtttaatgg aagcgctgta actggagttt
     1621 tggatgcgaa atgggccaaa gcgttatttg atatgagtgc ttttgttctt gttcccggcg
     1681 gagatagtaa aattagaaaa atgcagcaat ggttaaatgt aaattattta gaatacacgg
     1741 gaataatgcc ttgtgatggt atttatcaaa gaaattcaaa tcaagcatta atttttgcat
     1801 tacaagctga actaggatat tctcccagtg aagcaacagg gtcttatggt aatgggacaa
     1861 ctagtaaaac ttatcctgtg tctgaaggta actcgggaaa ctatgtaaga ataattcaat
     1921 atggattata tgttaatggt tattttgaag acggtacatt cgatggtatt ttcactaaat
     1981 atatgggttt agaagtactg gcattccgaa aatttatggt attgcctgaa tatactgaaa
     2041 tagcagatgt tgttgtaata aaaggactat tgtctagcgc tggagatatt agaagatctg
     2101 cagatggcgc agatacttct acgcaattaa ctagatctca aatccaaaca ttggttgata
     2161 atgatataaa aatagttggt agatatctta ctggtactgt tggaattgga aaagatgaac
     2221 gaaataagta tctaactaca gaggaattga ataatatttt ttctaaaaat ttatctgtgt
     2281 ttcctatcta tcaagatgga ggtgcagcat tagcttattt tacttatgat cgtggcttat
     2341 cagatggtaa gaaggctata gatgcagcaa aaaatctaaa tataccatta acaacagtta
     2401 tttattttgc tgttgatcta gacatgttag gggaagaaat tcttgcatat gctggagaat
     2461 attttaaggg ggtatctgct gtaatgtcat atagcgggta tcaaacgggt gtttacggaa
     2521 caagaaatgt ttgttctcaa ataatcaata acgggtatgc ttcgtttgca tttgtttcag
     2581 atatgtcgac tggttatagt gggaatttag gttttcctat gcctagacaa tggtcttttg
     2641 atcaatttgt tgaatttacc ataggaagtg gcaacggagc tgttggtatc gatttaatcg
     2701 ctacatcggg tagagatagc ggatttaatg agttatcaaa tgatactaat aatgataatt
     2761 acattgccaa atacaatcag aaggttataa atcagatggt acgtgcgtat caatatctat
     2821 cacaagctgg tcttgataat ccttggaacc ctcatttgac attttataga tatgtaaatt
     2881 attctggttt gtcatgggac atcatttctt ctcctgtaac agagcatgat agaaaaattt
     2941 atgatgaata taggaataaa ttaactaata gcgagggcct gtataattat tttatagatc
     3001 ctaatactgg ttcaataata ggtttgcctc acttaattgt gacgttacaa tcacagatgt
     3061 ttctagccga cacaatcaat gatagtgttt ctgactttgc aggttggtta ggagatttaa
     3121 tgacttgctg gggtgaagtt aagaaattag gaatacaaat ggagcaaggt gtatttgcat
     3181 tagtaggtac ggctgcaggt ggaacattta gtctagaaga tttataccaa gatgttgacg
     3241 cggtaaatgt ttatacctta gcaaaaagaa attctgattc aacagcttta tcggctttaa
     3301 aaacgtacta ttgtgataag ggtgctattg atagatttaa tcaatttgtt aagaacagat
     3361 ttggttcttc agataaaatc gcttcagtaa caaatagttt attagaaaat gacgaaacag
     3421 ttgatatctt aggtgtaggg ataatttcat ttttcattga taaaatagtt aataattcac
     3481 ttgggacaga ttttacttat cttgatttta ttggagatga ggggaaagtt gcaaatggat
     3541 tttctgaaaa aattattcaa tttcaagaaa atagaagtaa acttaattaa gaatcgttta
     3601 ctatttaaac agtgtccttt tcctttttaa atatatacga taaaataagt atatgaattg
     3661 taaaaggaca tgatgattat ggaagaaata gaatggaata atctgactca aaataataaa
     3721 aataattctg gatgtggaat tatctttatg tttgttatag ttttgggaat agtagtactt
     3781 ttaataactg tcaagatagt agattacttt gcgaatactc ctgaagaaca aatacagaga
     3841 aaacttcctg agtttgaaag agtaccacct tatgatccaa gagataattc atttcaagta
     3901 aaaaaattaa aagagtctcc aaatgaaatg tggtttgaag ttacagttga atcaagcgac
     3961 ttcaaagaaa aaaagaaaga atacaattat attgtaaagg caaaagtatc aaaaagattt
     4021 atttttaacc ctaaattaga aatatatagt gttgtaatag aataatagtg ttaaaaaaat
     4081 tgtaaaagtt aaagaagtca aattctgatg tgaaaaataa aatataccat ggaagacata
     4141 aaagaaaaac tggataacta gtttatttaa tggcagaccg ttaaggcctg ccatttttat
     4201 gtattttagc ggaataaata taatcatgcg ggagtgtaaa cttactgttt caaaaagtat
     4261 actttttgac atgccttgat tttctttgtt tatgtatggc ataataacca cataatacaa
     4321 tttgaaacgt ttctgattta atctgattgt tttgaaacaa ttttagtgaa aaaagggtag
     4381 ataaaaacga tgagaaaatt tttttatatc cgggtcagta gttatgatca atcgatcgaa
     4441 cgacaactga tttccgcgaa ggaattagga atccctgaag aatatatatt tgtagaaaaa
     4501 gccagcggca aagattttaa acgccctgaa tatcaattaa tgaaacggat gtttcgaaaa
     4561 ggagatttac tctatctaca atcattagat agactaggca gaaataaaca aatgatttta
     4621 gaagaatggc gagaattaac taaggaaaag cagattgata tttctgtgtt agacatgcct
     4681 ctgttagata ctgctagtta caagcaacta gatgggttag agacgcttat ctctgactta
     4741 attctgcaat tactcagcta tatggccgaa gacgagcgga aacgaattaa tagccggcaa
     4801 agagaaggcg tagaagctgc gaaacaaaag ggtgtaaaat tcggcagaaa gaaaattgct
     4861 ttacctcctg aatttcctgc gatctattct gactggaaag caggaaagat aactgcagtc
     4921 tatgctatgg atcaattggg catgaaacga aatacctttt atcgacgggt gaaagaatat
     4981 gaacatacct tgcaccactt ttcttgatag atataacagc tattagacgg gaagggagag
     5041 tttgaaaatg gacttaacga atcaaagggt cgtttttact ggaacgctgc agcaaatgac
     5101 acgaacccaa gccatcggtt tggttcactc tttaaacggc cattgtcaat caagtgttac
     5161 ttcaaaaaca aatttcctgg tttgtggtca gtattctaag tcgttgtttg atgattctct
     5221 ctatacaaaa aaggaacaaa ccgcgcggga cttgattgcg ttaggtcatg agattaccat
     5281 tttatcagaa gtcgaatttt atgcgttaat tagtcaacag ttgaaagaat ggcaacggta
     5341 tttataatcg ttagtaaaat ttacaaggag gaaaattttt atgaagcgaa aatcttatga
     5401 tcgttggggc attgaaataa ctagttttaa tcgaaatatt gcttttcacg tggagtgtga
     5461 ctgtggggaa ttagcaaaaa gtgtttatca aaaaaattac ttttactgtc ctaaatgcca
     5521 tcgccgatac ggggtacatg gaacatagaa acgaaatagt gcggtagaaa agcgaatgaa
     5581 tgtagtatat ttctacaaat cattcgcttt ctttatttta taaatagttg gtcgagagat
     5641 cccgttttct tttgcaatct ctgcaactgg aatgccttgc tttaacatgt ccaccacggt
     5701 tttaaaaact aagcgttttt gcggatctgt tgagttaata gaatactcga ttggtcgtcc
     5761 cttatatcgg ccggcttttt gagccaataa tactccttgc cgctgacgtt cacgaatttt
     5821 ctcacgctcg ttttgtgcaa tataacttag cagtgataaa aacatatcga acatagcggt
     5881 atccaggagt tcatttccag tattaaaatc taaaaactgt gcatctaata tttttaacgc
     5941 tacttttttc tgtttcataa aagcaactgt attttttata tcttcataat ctcttcctaa
     6001 acgatctagt gatgggttct gttgcgaagt ttgaaaatca aacgcgccct ctctgaactc
     6061 caaatatctt aggctgtta
//
LOCUS       DASHFF010000107         5978 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.109, whole genome shotgun sequence.
ACCESSION   DASHFF010000107 DASHFF010000000
VERSION     DASHFF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.109"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1127)
                     /locus_tag="U2I75_002674"
     CDS             complement(<1..1127)
                     /locus_tag="U2I75_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1697727.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1210..1545)
                     /locus_tag="U2I75_002675"
     CDS             complement(1210..1545)
                     /locus_tag="U2I75_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN1697728.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(1555..1929)
                     /locus_tag="U2I75_002676"
     CDS             complement(1555..1929)
                     /locus_tag="U2I75_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1697729.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(1942..2256)
                     /locus_tag="U2I75_002677"
     CDS             complement(1942..2256)
                     /locus_tag="U2I75_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1697730.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(2369..5596)
                     /gene="fss3"
                     /locus_tag="U2I75_002678"
     CDS             complement(2369..5596)
                     /gene="fss3"
                     /locus_tag="U2I75_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN1697731.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         1649 a         1307 c         1012 g         2010 t
ORIGIN      
        1 tgatcgcgga ttccgtctcc taaatatttc gcatccggcc tttggcaagc caaaatcaga
       61 aagaaaccag attgacgacc caacatcacg atttgtttga gcttattcaa aatcaccgca
      121 ctttctttcg tcgttaacat ctccatataa gccacgtatt catcaaagat taaaaagttg
      181 ggtggaagtc caagataggc ataattctct cctgttttgt agttagacat ttctttcatt
      241 gccttgctac gagccatcat acgttcatag aaatcttcca cacaagcaga aatttcttcc
      301 ttttgtgaat aaacatgagg catcaccgta cctaaatcag ctaaatctgc atttttggga
      361 tcgaggataa acaattctgc atctgacttc aatagtgctt cgataatggt gaggaggaaa
      421 taggtcttcc ctccacccgt gccaccagcg attaacatat ggggtaaaga atcataagcc
      481 catacttgat ttttcatcaa tcgcagagtc ccattttctg ccactacttc gtctatccca
      541 attcgattgg cgatcatatc atacagtaaa gtatactcca cataggagtc tttgagttcc
      601 ttttctacta actcacaata cagtccgctt tctaacttct tttccaattt taaaagctga
      661 tcttggtatt tcccaagtga aatttgaacg cgaatagaca gtaatccatc tttcattcga
      721 taatagattt tggggaagta actgatagtc tctctggttc ggctactatt caaatctttg
      781 aaaaagcctt cactttgagt ttgtttcact tcataccaat ggttctctag aatcatccga
      841 gctagctttt gtcgatgcca caagcttcgc caatgatcaa gaaaaaagtg aatataaagc
      901 aatatcatag ctcctgaaaa agcgagactt ccaagcagac atactaaatc ttttatagac
      961 caaacgattt caatagtaga aagtgatagc ttagaaatat caaattgtct aaactcttga
     1021 aaataaaaga cgctcatcca accgatgaac agagcgagta gccagccaag aacaaagtga
     1081 taaaccaagt gctgatctcc tgcgcgtatg cgatgtcctt tgtacatttt gatggtttcc
     1141 acctccaatc gaaaaagccc aataggattt cattaggcaa tttcaaaaag ttatgctagt
     1201 catttactgc tacgaggagg ggagttgttt cgtttcaacc aattgattta aaaagccttc
     1261 aatagcatcc ggcatctcca caccaatttc ttccaaaatg gattctgcct gccgacataa
     1321 gttttcattt acttcgacaa ctataaagaa agactcttct tctcctaacc agtctagtac
     1381 atcagagtct tcttcactgt cgctattttc tccaagtaaa ccttgatgta taatcagaaa
     1441 cattttttca tcagaggtta atgctttaac gagttgttcc tcagtcagct cgccttttcc
     1501 atattgatcg agacaatcta aaatatgttc aacattaatt tccataatct tcacctattt
     1561 ctttctttca ttttggttcg taggatttcc ttgattcggc tgccgcttca agactaaatc
     1621 atccgcctta atgaaccaat taacggtagt tccttctcga aaaacataat tggcaacggt
     1681 atccacgatt ggattgacca actctacctc tgcatcataa gcaaactcgc gtaagggtac
     1741 attagctggg atactaactt gaatcattcg cccttgtgca gaggatttta aattataggt
     1801 tcgttctttt acctcattac caattgttcc atcctcattt tgcttccgta cttctcggcg
     1861 taatgcggaa aaacgcaact tcccaaatgt taaacctgaa tctacgacaa taccttctgt
     1921 taatctcatc ttcttttcct cctatgcttt cactaaatca tcaacataca acacgtaatc
     1981 agtaaaccca cgattctcaa tctcatagcc tttggcaacc aaatggggat tcacggcttt
     2041 tagcggttgg tcttgatcaa aatccttttc accggcttcc gcagctacta ccacctcaat
     2101 atcatcggcc cgttgcttac tggaatacaa atgataactg cgaccaatca ccgtagtatt
     2161 ccgtccatat ccttctgtta taatttctcc ttcaccggca taacttaact ttccaaaggt
     2221 ttcagccata tctggcacga caaatttcaa ttccatcttc ttttcctctt ttcattgtta
     2281 gttttggata caaaaatagc gcgactactt ttttaggagg cggtcgcgca cgttggttct
     2341 taggatttac ttgtatcatc tcatcacctt agttccggcg attccagaaa taatagcccg
     2401 ccgtcgcaaa aatcaaagta aaaccaatga acagtaaaac attggaattt ttctccccgg
     2461 tttgagggaa ggtcttcact gtgggactag attcttgaga gccacttgga acagttggtg
     2521 tttccggttg tttcggcgta cttagtatgg ttggaacttc ttttggtgtt aaggtttgag
     2581 atttttcttt caaatcttca ttgtgttttc cattgatatt cccatcttta tcgtaattga
     2641 tttccgcaaa agtaaacttg gttccttctg gatattttcc ggtatctact tttttcgcaa
     2701 gcacagtttt ggtgaattct ttatcattta cttcataatc aatcttgcca gatttccaaa
     2761 tttctttgtt tgtaccatcc ggtaataaag catacagaat tgtttcgaaa gcttcttttg
     2821 agccatccag tacatcatga gtaatcgaca catcatcaaa catatccacc acatcaccat
     2881 gagtaaaggt ctgagaaccg ttttctaggt gggcctttgt ttggatagaa acatggtgtt
     2941 ccactgtaca actgacagtt tgggcttggt tattcaagct aacatccttc gctactggct
     3001 tatcgcctgc ttcaaaagct tccttatttt catacacata gttgaataag acaatagttt
     3061 tatttaaaac ttgttcggcg gttaatttat gctgaatttt ccaagttcct gttttctctt
     3121 tgttcgccaa cgttgcgctt gtttctgcaa tcaccactgg tttggctttt tcatcttttt
     3181 cttgggcagc ttttgtcgca tccacatcaa tggcttgcgc taccacatac cagtcttctt
     3241 tgatttcatg tagattgtaa ctcaatttat caactagctc ggtagaatcc agagaagtta
     3301 attctttgtt tccgtctttc caagtcgcaa gagaagttaa actatcttct tcctcaggca
     3361 aatcatagag cattaaacgg ttcaaactaa cagaaaatgc tttgttcgtc agcttctcgt
     3421 aaggaacggt caccgtttta attggttgtt tttggtcttg ttcggtaatg gtaaagacat
     3481 actcactctt cacaaagtct tctttgtttt ccttaaaagt agaacggatt tctaatggcg
     3541 taatcttttg aaaaccttct ggggcttcta cttcttcaag taaataatcc ccataaggaa
     3601 gattttcaaa cttgccataa ccatcaaacc ctaattgctc gttataagct gtggtcgctt
     3661 catcttcggc acctgtgatt tcattggtcc cttccaatgg cgacacttta aaggtcagat
     3721 cattgaagcc agtttcggta gtgcccgcag ctgatccagc aaatttaaag aaatcaaagc
     3781 caaagcgaat cacttgttct tttgccgtaa catttcgggt aatcacggca tttttttcgc
     3841 tatcatccac ctttttgatg gatacaggat actttgtttc atcaaggcta tatccttcgg
     3901 gcgcttttgt ttcttgccaa taatactcgt tgatagctaa atgtttaacg gcaacttggt
     3961 tcttttcgtc taaagtcaaa gtgaccgttt catcagaagc tttcgttccc ttcgctaact
     4021 ctggtttaaa agcctcactc catttaacag cttttccatc cttagcagta aacaaggtgt
     4081 attcagcacc ctcaaagact gccttacctt gtgctttatc cccagtatct ttgtcttctt
     4141 tcgtcaaagt ggtttcgcca gtgacttctt gattttgccc ttttacgtta ctggtaacaa
     4201 gggctacggt ttgattggca tacttcaatt cgacttttac aggtttgaac gtattcacga
     4261 acccatgact agctttagtt tcggtcacat aataggttcc caattccaac gcattagcaa
     4321 tctcttttgg tgtttccgca tgaccgtttt catccgtggt catttcttga acgatttcac
     4381 cggttggact gtctttgcga atggcaaacg tattaccagc taaggaataa ttgtcattcc
     4441 aaagatcact ccctgtttca actccgcttt tatccagaat gatttgccct ttttcccgtg
     4501 cgttttttga agtcacgtaa atggtttcgc ctgctttaat ggtggtagtc atgggggtgg
     4561 tatcaatcgt ataaggcgcc ggtactgatt tttcggtaat ggttactttt gtaccatgag
     4621 gaatctcatc caatgtagca atgccttctt tgtcagtagt cacgtctttt gcaggtaaag
     4681 tttttccaaa gtctaaatgg aaaaccgttc ctggtacaac atcacccgat tctttatcga
     4741 cttttttgat ctttaaagaa ccctccgttt ccacatcaat tttgattgtg taggtattag
     4801 gtttatcaat cgccccggcc attaaagtct gttggccagc ctttttatag gcgacaggtg
     4861 ttccggtacc agcagatttc ttaagcgtca gcacaccact ttcttttgaa ttggcatttg
     4921 gggtgattat caactgatta ccattcacac gataatcaat gtttgcggca ttttccacaa
     4981 cctcatcgaa ctctgacaaa ttcagattat tcgtatccgt cacagtagtc gattgcccta
     5041 atactgtttt agccgtacta ttatggaagg ttggtttctt ctgataatcg tcaatggctt
     5101 gatttatttt tgcctcaatg gctgcgatat ttacggcact accatctgat cgttttattg
     5161 agtgaagggt atacccatta acttcttgcc agatcatctt ctgtgccaca atatgagtat
     5221 ctgcatctgt acctactttt ttccataaaa cggaaactaa tttggcttta tcggacatat
     5281 ctggtaacgg gtttttctca tagccaggag tgaactcatt gtaaatcggg actcctggtt
     5341 caatacagaa aatatcttgt ttaaagccgt tgtacactgc ataatgcgga acagtgactt
     5401 ctgacattgg acgaccatca ctaaaagttc cttcaacagc atattgatgt gctaaatcta
     5461 gttctacggt taccgtttgt ggatgggtaa tttcatcagc aaaagtaatc gtaggagcaa
     5521 ttgctccaac aattgcttga acagccaaac tcaaaatagt taatacacat aatttctttt
     5581 tttgtttttt atacatgaaa tgttctcctt tcaaattaag cacaaaaaaa taagatgctc
     5641 gcttgaacat ctttgtttat aaagtctttt taaaattttt attgactgaa tagaatggat
     5701 ttgagaatga atgagcctaa aaatttaatc taaaatacga aatatactgc ctttaaatta
     5761 gaacacattt tagaacacaa acatttcaaa tttaatcaaa tataataaaa tatatttacg
     5821 ggaaaacctt tataaatcaa ggtttttcaa acttattcaa tgatacgaag aaaaaaccag
     5881 gtcttaagaa agctcgtaaa gcatctcaat tctcaaaacg ttaatatcct ttatatatca
     5941 acgttcaaga cgctttccaa ttatttggaa agtgtctt
//
LOCUS       DASHFF010000108         5704 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.110, whole genome shotgun sequence.
ACCESSION   DASHFF010000108 DASHFF010000000
VERSION     DASHFF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.110"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(166..687)
                     /locus_tag="U2I75_002679"
     CDS             complement(166..687)
                     /locus_tag="U2I75_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN1697732.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(690..1532)
                     /locus_tag="U2I75_002680"
     CDS             complement(690..1532)
                     /locus_tag="U2I75_002680"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN1697733.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(1525..2250)
                     /locus_tag="U2I75_002681"
     CDS             complement(1525..2250)
                     /locus_tag="U2I75_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN1697734.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            2426..2722
                     /locus_tag="U2I75_002682"
     CDS             2426..2722
                     /locus_tag="U2I75_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697735.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            3290..4882
                     /locus_tag="U2I75_002683"
     CDS             3290..4882
                     /locus_tag="U2I75_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1697736.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            4895..5635
                     /locus_tag="U2I75_002684"
     CDS             4895..5635
                     /locus_tag="U2I75_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1697737.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         1626 a         1120 c         1154 g         1804 t
ORIGIN      
        1 tttctccact aatcgaaatt tagatagaca tctagaaatc gcaattccaa aaaattctgt
       61 gtattggata cgcatctttt cttatttttt cttatttctt ccatatgggt gtttatctca
      121 gagatgatag aatagtttct aggataaaaa gtagcagcta cgatgttatc cttcgtagag
      181 gattcctgct tcagctagtt tttgtttgat gtttttcagc atatgctttt ctgggtagcg
      241 gatcgtatgc agatggatgc cgcctgttaa ttcggataac aaagtagcat tacttttttg
      301 ataacgtctc ataaaggaat cgatatcttg tggcgttttg atgtgcaaag ttcctgctaa
      361 ctcaccgtaa aggggatgtt ccacaattac atcaatgatt tcaccaccat tttcaacgat
      421 tagtctcagt tcttcttctg tttgatcttt tccatgctgg acagcgattt tactgatgtg
      481 cccagtattg gtttcttctg tttcgaggat ataacctctt gctgttgcca cgatactttt
      541 acctgcagct cgtagtaaag caatatcgcc tacaatgatc tgacggctga ctccaaaagt
      601 tttcgcaaac ttgctagcac tcactggttt ttctgtattt gccaaggttt gcatgatttg
      661 ttttcgccgt gtctctcctt cgatcacact tattcctcct tcattttgtt ttggtattcg
      721 cgcgagtgtt tcatcaaatc aacatacgtg tctgttattg tttccttata tcccttattt
      781 ttcagcagac taacaacttt atccaatact tgctgctctc tttttgcatc ataaactgct
      841 tggttttctt cttttttcac ttgtcctatt ttagttaccg tatccattct tttttctaat
      901 aaagcaacca actctcgatc gatcatatcg atcttatgac gtaatgtttc aagagactct
      961 tctgttagtt cttcctctac tgtcttgatg atacgagcag gatttcctgc aatcactaca
     1021 ttgtcgggaa aagatttggt cacaaccgaa cctgcgccga ctactacgtt gtcccctaga
     1081 gtgacacccg gcgtgacgat aactcctccg cctaaccaaa cattattacc gattttaatg
     1141 ggttttgcgt actccagacc gctgttacgt ttcactggat gtagtggatg cgtcgccgtg
     1201 taaagctgga cattcggagc gaacatgcag ttatcaccga tttcaatcgt gctgacatct
     1261 aaaaatgtac aattgaaatt tgcataaaaa ttctccccaa caaatatatt atatccgtaa
     1321 tcaaaattga taactggctc catatagatt tttttccctg tacggccaaa agtctctttc
     1381 aacaattggg atcgcagttc cgctgattct gcttggttga tgatttggct ttgacttctt
     1441 gcaaacatac ggtcggcaac cagctcagga tcattggcaa aataaagctc gcctgcaatc
     1501 attttttctt tttcggtttt gaattcagtc ataatctgct cctaattctt gtattttttt
     1561 gaaaacaacc gctccgtctt cttgaataaa cgtataaggc tccgctaaat aaacaggcgt
     1621 ttgattgatt actaagattt tggctgacgg ctggcgaaac tgtaccagat cacaaaacgg
     1681 atgaacttga aaactcgttc caacaatgac gatcagctct gcttggctga cggcataagc
     1741 cgcctgctcc atgactccat cttgaaaccc ttcaccatat aacacgatgt ctgggcgtat
     1801 ttgtcctccg catgcttgat gtttgtcgct gtgaagatat tctttccagt ctaccgtttg
     1861 ttgacatttt cgacaatgac attgatacag actcccatga aaattgacga gttgcgggct
     1921 tttagccttt gcgtgcaaac cgtcgatgtt ttgggaaaca acccaaacat ttttttcttt
     1981 cgctagatgt gctatttcca aatgaatgat attgggtttt gcttcaggat gataaatctt
     2041 tttgacaaat tgatagaatt tatccggttc attcaccatt gcttgatgac tcagcaagta
     2101 ttccggctgc tccatcccgt ggtagattcc tgacaaagat cgatagtctg gcactccaga
     2161 aggtgtggat acccctgcac cggtcaaaaa tgtaatttgc tgataagcta atatctcttt
     2221 tgctgcttcg tctactgtta attgtttcat gttttgtcac ctcactttcc attatagccc
     2281 atactcgaac aaatttattg ttgaaaagtc agaaaaatgg gaataaaaaa gtgttatggg
     2341 catttgagca atttatcaca atgttcactg acaaaagaat gaaaacgtgt tacaatactg
     2401 ctgtataaaa gtgaggtgaa tcgatatgtt accattcttt atttattggg gcgttattct
     2461 ggcttgtatc gcctggttag cgttaagcgt ttatttctct gttttttatc ttgtacgcaa
     2521 agaaaatgga aatctttggg cttttgcttt attcaacgtg attgccgcaa ttgttttagc
     2581 cattacatta gccgtatatc gaacatgggg ctggggaatc acccaatact caagtttgat
     2641 ttatctaata ttagccattt atggcgtcgt tgttcttttg caagcgatcc taggacgtga
     2701 gccaaagaaa gcggcagctt aaatgatcga gatggaactg gacacaggaa attttctgtt
     2761 ctagttcttt ttttattgta ttacatacat ttttgtgtat aattatacta tcgtttgcaa
     2821 tggatagatg attcacattt tgattgtttt ttcggtaaaa gtaaagattt ctatatttat
     2881 cagtattttt tcaaaaacca gcagttatag attgacaaag cctctcgagt atcgtaaact
     2941 ttttttgtat aaataactga atacgagatg cctcaatcaa tgaggtagag gtcgcgagaa
     3001 tcattaaccc tgtggagtga agcaaacatg gagaagcagg aatagggagg atcgccgaaa
     3061 tgtgcacttt tttgcttgga gtgtatgttg ggacgcaagt taagagcttg cggactgtct
     3121 tagcagtgat gctaagttgc gctatgtttt gtgagaggtt tatcacctta tggaatttta
     3181 gcggcctttt tatatctata aatcggtcgc tttttatttt ttcagataaa gctttaccgc
     3241 atttcatcta gaccgctcgt gtatcatata atcagaaagg gagaaaaaaa tgaaaaaaaa
     3301 tatggtttca gttgccgtac tatttgcttt tgtcttctca ctattcagtg tgggatctct
     3361 ggcaaatgca gatgaaaaaa caccgagcga tgagtttcgc gtaggtatgg aggctgggta
     3421 tgcacccttc aactggtctc aacaaacaga tgagaataat gccttgccaa ttcaaggcca
     3481 aaatagttac gccggcggtt atgatgttca aatcgctaaa aaagtagcag atggtttagg
     3541 gaaaaaatta gtcatcgtcc aaacgaaatg ggatggtctg gctcctgccc ttcaatcagg
     3601 caagatcgac gcgatcatcg ccggaatgag ccctactgct gaacgacgaa aagaaattga
     3661 ttttaccgat ccttattacg aatcacaatt agtagtagta gttcaaaaag atagcaaata
     3721 tgcaaaagca aaaagtttag cagatctttc tggagcaaag atcactgctc agttaaatac
     3781 attccactat actgtgatcg atcagatacc agatgtgcag aaacaacaag caatggataa
     3841 tttctcagca atgagaacag ctttagcttc tggaatgatc gatggttatg tcagcgaacg
     3901 tccggaaggt gtaacggcaa ctagtgtgaa caaagacttg gaaatgcttg aattcagcgg
     3961 tgcagatggc ttccagacaa acccggaaga tgttcagatt tctgttggta tgcgtaaagg
     4021 cgacccggat atccaaaaag taaaccagat tctttctggc atctctaacg atgaacgaac
     4081 caaaattatg gaccaagcag tcaaagatca gcctgctgca actgatagtg aagaaaaacc
     4141 tggcatcatt tctgatttca ctacgatttg gaaacaatac ggcaatatgt tcttacgagg
     4201 tgctggactt accttgttca tcgcattgat cgggacagtt gtcggtacta cacttggatt
     4261 actgattggt gtcttccgtt cggtacctga atcaggaaat gcactgactc gcttcttcca
     4321 aaaaatcgga aatgcacttc tttctatcta tgtcgaagta ttccgcggaa caccgatgat
     4381 ggtacaagca atggtcatct tctacggtct ggcactcgct tttggcattt cattgaaccg
     4441 aacgattgcc gctttgttta ttgtctctct caatactgga gcttacatga cagagatcgt
     4501 tcgtgggggg atttttgcag tagatgaagg gcagttcgaa gctgcgcaag ccattggtat
     4561 gacccatggc caaacgatga gaaaagttgt tattccacaa gttttacgga atatcttgcc
     4621 agcaacagga aatgagtttg ttatcaatat caaagataca gcagttttaa gtgtaatcgg
     4681 cgtagctgat ttgttcttcc aaggaaactc tgcttccggt gcgaatttcc aattcttcca
     4741 aacatttacg atcgtcggga tcatctactt agtcatgacc tttacgatca ccagaatcct
     4801 gcgggcggta gaaaagaaaa tcgatggtcc ttcagcttat accaagcttg aagaaatcga
     4861 caattcaaat ttgcaggaat aaggaggaag aatcatgaat gaaaaaatta ttgaaatcaa
     4921 acatctaaga aaaagcttcg gtgaaaatga agtattgaaa gatatcagcg taacagtcaa
     4981 taaaggtgaa gttgtgacga tcatcggttc ttccggttct ggtaaatcta ctttcttgcg
     5041 ctgtatcaat ttactggaaa aaccaacagg cggcgagatc atttataatg gagaaaatgt
     5101 attagctcct aaatataatt tacctaaata tcgaacgaat ctaggtatgg ttttccaatc
     5161 cttcaacctg ttcaacaata tgaatgtctt ggaaaactgt atgtctggtc aaacaactgt
     5221 cctgaaacga gataaagaaa ctgccaaaaa agtagcaatc gaaaatctgg aaaaagttgg
     5281 tatggatcgt tatatcgaag ccaaaccagc acaattatct ggtggacaga aacagcgtgt
     5341 cgcaattgct cgggcattat caatgaatcc agatgtattg ctattcgacg aacctacttc
     5401 ggcgttagac cctgaaatgg ttggcgaagt gcttaatacg atcaaagact tagcacatac
     5461 tggcttgaca atgcttatcg tgacgcatga gatggaattt gcaaaagatg tatctgaccg
     5521 tgtcattttt atggataaag gtgtgattgc agaagaaggt acaccagaag atatctttgt
     5581 ccatccgaaa gaagaacgta caaaagaatt cctttcacgt attttaaatg cttaggatca
     5641 atagctgatt gggacaaaga gctgcggtgt ttaccgcagt tcttttttat gaattactca
     5701 taaa
//
LOCUS       DASHFF010000109         5689 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.111, whole genome shotgun sequence.
ACCESSION   DASHFF010000109 DASHFF010000000
VERSION     DASHFF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.111"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(488..1297)
                     /locus_tag="U2I75_002685"
     CDS             complement(488..1297)
                     /locus_tag="U2I75_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1697738.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="U2I75_002686"
     CDS             complement(1297..2043)
                     /locus_tag="U2I75_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1697739.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="U2I75_002687"
     CDS             complement(2061..2528)
                     /locus_tag="U2I75_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1697740.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="U2I75_002688"
     CDS             complement(2528..2938)
                     /locus_tag="U2I75_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1697741.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="U2I75_002689"
     CDS             complement(2949..3962)
                     /locus_tag="U2I75_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1697742.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="U2I75_002690"
     CDS             4179..4913
                     /locus_tag="U2I75_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1697743.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="U2I75_002691"
     CDS             4897..5607
                     /locus_tag="U2I75_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1697744.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DASHFF010000110         5560 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.112, whole genome shotgun sequence.
ACCESSION   DASHFF010000110 DASHFF010000000
VERSION     DASHFF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.112"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            181..495
                     /locus_tag="U2I75_002692"
     CDS             181..495
                     /locus_tag="U2I75_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1697745.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            516..893
                     /locus_tag="U2I75_002693"
     CDS             516..893
                     /locus_tag="U2I75_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1697746.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            903..1676
                     /locus_tag="U2I75_002694"
     CDS             903..1676
                     /locus_tag="U2I75_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697747.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1698..3101
                     /locus_tag="U2I75_002695"
     CDS             1698..3101
                     /locus_tag="U2I75_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1697748.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3283..4467
                     /locus_tag="U2I75_002696"
     CDS             3283..4467
                     /locus_tag="U2I75_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1697749.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4464..4754
                     /locus_tag="U2I75_002697"
     CDS             4464..4754
                     /locus_tag="U2I75_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697750.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4751..4972
                     /locus_tag="U2I75_002698"
     CDS             4751..4972
                     /locus_tag="U2I75_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697751.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5014..>5560
                     /locus_tag="U2I75_002699"
     CDS             5014..>5560
                     /locus_tag="U2I75_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697752.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKL"
BASE COUNT         1880 a          941 c         1101 g         1638 t
ORIGIN      
        1 ttttaaatat agtaggaatc ggacgctcag tacggagcgt ctattttttt acccaatttt
       61 gaaaggaagt gaacttatga aaataaaaaa tcaagaatca aaaggtcgct ctcccccctt
      121 taaaaccatc acacaaataa gattcattca aacataaaaa cagaaaggat aggtaaaaat
      181 atggaactta aatatgtcat tcccaatatg gaaaaaacat ttggacaatt agaatatgct
      241 ggtgaaggga acattgaaca acgtcgaata aatggcagac caaccattct ctctcgtagt
      301 tacaatttat actcaacgat tcaacgagca gatgatattg ttgtcattct tccctccgaa
      361 gctggcgaga aacattttga atccgacgaa aaagtcaaac tcatcaaccc acatattacc
      421 gcagaaggtt ataaaattgg caatcgagga tttaccaatt acattatgca cgcagacgat
      481 atggtgaaag cataagaaag gaaaggtgaa aaaatatgag attagcagaa ggtattgtga
      541 ttgaaaaaga ccagacattt ggcacattga aattttccgc cttgcgtcgt gaggttcgta
      601 tccagaatga agatggtacg gtttccgaag aaattaaaga acgtacctat gacttaaaat
      661 caaaaggaca aggacgcatg atacaagtca gtatccccgc ttctgttcct ttgaaagaat
      721 ttgactacaa cgcagaagtg gaattaatca atcctgtggc agataccgta gcgacggcta
      781 cttttcgagg ggcagacgtt gattggtacg tgaaagcaga cgatcttgta ttaaaaggac
      841 agacaaaacc tcatcagaaa tataaaaaag aagcgtccga aagtgaaaaa taggtgttgc
      901 ttatgacaaa cacaccattg attttgaaag aaattccgaa ggatgaagcc atttctttta
      961 ttcggcaata ccattattca aagatactcc ctagattgtg taaatacttt ctagggattt
     1021 tttcagaaga aaaattatta ggcgttgtgg aattaggctg gggtacgcaa cccctacaaa
     1081 ccatacgcaa actctttccc gacagttccc ttcaaaccac agattattta gaaattggaa
     1141 agatgtgttt tcttccagaa atgaatcaaa ccaattattt tggcagtcaa gctctttcag
     1201 ctctaattaa gtggttgaaa gaacatacgg actgtcattt tttatataca ctggcagatg
     1261 gaattgaggg aaaatgtggc tatgtctatc aagcgtccaa cttcttttac tgcgggtatt
     1321 tcaagacaag tgtctatcgt gataagcagt catgggaaaa gatacaccct cgaagtgctc
     1381 gccttttatt agaggaaaat gctcgctttg aacaggtaga gaaaaagcat tggctttccc
     1441 aggcattttg cgaatacaaa gggattgaga aaatcaatgg acgcatgttt cgctatctct
     1501 atccgttgac aaaagaagcg aaaaaactgt taggacacac tctctatcga cgtcattatt
     1561 atccgaaaga aaaaaattta cgctttgaaa agcgaatcgc ttatcagaaa tatgaagcca
     1621 tttctcaacc aacttttgat aaacaggctc gtatctacaa tacacaatta ttttgatagg
     1681 tggaaaggag gatttttatg ccattgcttc atgacagagg agagcgaatc aaagccaacg
     1741 ataagcactt ggtctatcat tttgtactaa tttcattgct ggtagttttt ctagtcgtcc
     1801 tactcccttt tcattggaga gcgttacaat cggtgaactg gcaagcagtt cagttagata
     1861 catttttcta tcaacttcat acgccatact tttggagcag tattctcact gctctttttg
     1921 tatgcggact tatcagctgg ctttattatc actatcgtat agattctata aaacagttag
     1981 agcaccgtca aaagttagct cgtatgattt tagaaaatca ttggtatgaa gccgaagaaa
     2041 ctcaatcaga gggattcttt aaagatttac ccagcaagaa aaccaaacaa cgaatccgcc
     2101 attttcctaa aatatattat cgaatgaaag atggactaat ccatattcat gtagaaatca
     2161 ccttagggaa atatcaagat cagcttttgc acctagaaaa caaactagaa acaggattgt
     2221 attgtgaatt agtggaaaaa acgttgaaag attcttatgt agagtatgtc ctactttatg
     2281 atactattgg caatcgaatt ggcattgaag acgtggtagc tcaagatgga cgtgtcaaat
     2341 tgatggaatc ggtctattgg gagttcgatt ctctccctca catgttaatt gctgggggca
     2401 ctggtagtgg aaaatcctat tttattctca cattgattga atctctcctg catacaaatg
     2461 caaagcttta tattcttgac cctaaaaatg ctgatttggc agacttaggg gcggtcatga
     2521 ataatgtcta ttaccgtaaa gaagatatgt tggcatgtat caatcaattt tacgaagata
     2581 tgattgatcg cagtgaaacc atgaaacaac accctaacta taaaacagga gaaaattatg
     2641 cttatcttgg tttgtctgcc aattttctta tttttgatga atatgtggca atgatggata
     2701 tgctaggtcg tgaaagtacc accgttattc ataaactcaa acaaattgta atgttaggac
     2761 gacaagcagg cttcttttta attcttgcct gtcaacgtcc agacgcaaaa tattttagtg
     2821 atgggattcg ggacaacttt aacttccgtg tggctttggg tcgtatgtct gaaatgggct
     2881 ttggtatgat gttcggaagc gatacacaaa aacaattttt cttaaaaccc atcaagggaa
     2941 gaggttacgt ggatacagga aaaagtgtga ttagtgagtt ttatacaccc cttgtaccaa
     3001 aaaggtacga tttcttagga gaaataggaa aagtcattca aaaaaagcaa tctgagcctg
     3061 taccgcacga agtgaaaggt acaggttcag acttgctttg attggtgtgg cgttagccac
     3121 gccaatcctt tagccccctc tatctaacag gggggcacaa atacaactga aaacaggcaa
     3181 ttctcatcaa ccaatgttga tggtacaaag gattgcggaa aatatgaaag gtgtaaactc
     3241 aagggtcata gtttacatct tttttagatt ggaggtttgc gaatggaaaa cgaaaaaatt
     3301 tggattaagg aattgaaaga aaagcgactg gcttatggcg tttctcaaaa taaactggcg
     3361 gttgcttctc atattaccag accttaccta tcggatattg aaacaggcaa ggcagtccct
     3421 actcaacagg tgaaagaaga tttactgaac gctttggaac gcttcaatcc cgataaccca
     3481 ttggagatgt tattcgacta tgtacggatt cgattcccta ccaatgatgt ggctcaaatt
     3541 atcggtgagg ttttacgttt gaatatggat tatatgctac acgaagactt tggctattat
     3601 tcttatcccg aacactatcg tttcggtgat attgttgtcc tagtctctca tgatgtaagt
     3661 aaaggtgttt tattagaact gaaaggaaaa ggctgtcggc aatttgaaaa ctttctattg
     3721 gctcaacatc ggagctggta tgatttcttt caagattgca tggaacataa aggtattttc
     3781 aaacgcttgg atttagctat caacgataaa acagggattt tgaatattcc agatttggca
     3841 aagaaatgta aacaggaaga atgcatttcc gtctttcgaa gctttaaaaa ttatcgcagt
     3901 ggtgaattgg ttcaccgtga tgaaaaacca tacatgggaa acacacttta tattggctca
     3961 ttaaaaagtg aagtctattt ctgtatatat gagaaagatt atgagcaata cgtgaaaaat
     4021 gatgttccct tagaagacgc agaagtaaag aatcggtttg aaattcggtt aaaaaatgac
     4081 cgagccaccc aagccatgaa agatttattg gcacatcaac aggcggaaaa aacagccttt
     4141 gaaattatca atcgctatat ccgctttgca gataaagacg acactaaacg tcggagtgat
     4201 tggaaaacta atgaacgttg ggaatggttt atcggtaaaa atcgtggagc attacgccta
     4261 accacacagc cagagcctta ttcttttgaa cgaacattga actggttaca tcatcaagtc
     4321 gcccctacat taaaaatcgc aagcatttta gatgttctca acggtacaac cattatctcc
     4381 accatgattc aggaagccaa actgacagaa aaacatgaga aattaattga acaacaacat
     4441 ctagccatgg aggatttgat tacatgagcc ttttaacaaa agaattaaag aagttagggt
     4501 ttcagtgtgg cattgagttt caagcgtata tacaaaatac agggaaatat acgtctctca
     4561 ttattgaggg aaaacggcaa gctggtgata ccatctatac ctatgatttt tacaaggtga
     4621 gattttataa taattatacc aatcgagtaa ccgtctatgg agagcattta accccttttc
     4681 aattattaag gcgtgtaaaa agctatatct attatcgaga aaaatatctt aaggaaagga
     4741 ggacaatcac atgaattttg ggcagaattt gtacaactgg ttcttggata atgcacagtc
     4801 attggtattg ttagcgattg ttgtcattgg actgttttta ggttttaaac gagaattttc
     4861 aaaactcatt ggcttcttga ttattgctct aatcgccgtc ggacttgtgt tcaatgcagc
     4921 tggcgtgaaa gatgtgttat taaacttatt taaccgaatc attggtgctt aacagatatt
     4981 tttcatgata aaataggaag tatatatttt gttatgaaag gatttatgaa tatgtttaca
     5041 aatgatcaat ttaaatctct tttaattaaa acattagaaa gaaagagctc ttcaaattat
     5101 tatgaaggag gtaacgagat tgtttctaaa atgaaaatat cagaggtaac tttagatgag
     5161 actgacgatt ttacttatta taaaggatat aaaaagggct ggaatacgct ttgcacatat
     5221 cttaaaataa gagttccttt tgatgattta gacttttttg aaagtcataa agatttgatt
     5281 acccagacag caagctccat atatgataaa caaggtgata atgtattagt ggatactatt
     5341 ttagtaccac ttccagagaa ttacgaagta attaacttta gtcagttgaa aattagtgat
     5401 gtggtttcac aagctattga agacgctgag tcttttatga gtaatggtga atatcaacgt
     5461 gcctttgaca gagttcacac tgcttttcat ggatatttaa ttgaaatact aaaaaaatat
     5521 gaaattacag ttcctagaga cgaaaatcta tctaaattat
//
LOCUS       DASHFF010000111         5343 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.113, whole genome shotgun sequence.
ACCESSION   DASHFF010000111 DASHFF010000000
VERSION     DASHFF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.113"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(62..280)
                     /locus_tag="U2I75_002700"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="U2I75_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="U2I75_002701"
     CDS             complement(546..740)
                     /locus_tag="U2I75_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697753.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="U2I75_002702"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="U2I75_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="U2I75_002703"
     CDS             2092..3132
                     /locus_tag="U2I75_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1697754.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="U2I75_002704"
     CDS             3956..4288
                     /locus_tag="U2I75_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN1697755.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="U2I75_002705"
     CDS             4907..5248
                     /locus_tag="U2I75_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEN1697756.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDNEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1944 a          728 c          899 g         1772 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattaa
     4861 tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagataat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 ttt
//
LOCUS       DASHFF010000112         5198 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.114, whole genome shotgun sequence.
ACCESSION   DASHFF010000112 DASHFF010000000
VERSION     DASHFF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.114"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            24..695
                     /locus_tag="U2I75_002706"
     CDS             24..695
                     /locus_tag="U2I75_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1697757.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            754..1194
                     /locus_tag="U2I75_002707"
     CDS             754..1194
                     /locus_tag="U2I75_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697758.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1206..1874
                     /locus_tag="U2I75_002708"
     CDS             1206..1874
                     /locus_tag="U2I75_002708"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1697759.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1938..2561
                     /locus_tag="U2I75_002709"
     CDS             1938..2561
                     /locus_tag="U2I75_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN1697760.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2617..3003
                     /locus_tag="U2I75_002710"
     CDS             2617..3003
                     /locus_tag="U2I75_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN1697761.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3216..4607
                     /locus_tag="U2I75_002711"
     CDS             3216..4607
                     /locus_tag="U2I75_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN1697762.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1727 a          861 c         1095 g         1515 t
ORIGIN      
        1 atgatgaaaa aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg
       61 agcgatctac atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac
      121 cagagttcca tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg
      181 ctgttttgca cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt
      241 ctcttgaatc ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata
      301 caaacgatgg gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa
      361 tagatgacga aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt
      421 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
      481 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      541 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      601 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      661 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      721 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      781 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      841 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      901 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      961 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
     1021 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
     1081 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
     1141 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
     1201 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
     1261 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
     1321 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
     1381 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1441 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1501 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1561 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1621 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1681 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1741 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1801 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1861 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1921 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1981 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     2041 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     2101 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     2161 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     2221 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     2281 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     2341 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     2401 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2461 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2521 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2581 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2641 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2701 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2761 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2821 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2881 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2941 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     3001 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     3061 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     3121 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     3181 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     3241 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     3301 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     3361 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3421 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3481 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3541 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3601 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3661 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3721 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3781 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3841 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3901 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3961 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     4021 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     4081 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     4141 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     4201 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     4261 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     4321 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     4381 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4441 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4501 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4561 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4621 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4681 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg
     4741 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4801 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gtttaagaaa
     4861 gaggtcgtga gcctgccgtt tcactctgag tcacaaagag caatttcaaa aaatagcttc
     4921 tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg tcaggagaac accgtttacc
     4981 agaggttgtg acttgttttt agtcacgacc tcttttttta tttattccat ataaatacac
     5041 attatttatt ccgaaaagtg aaagcgcaaa taacaaaaac aaccaactta tttattatta
     5101 aagaaaagcg tatttaagta ggacgattct gttgagagct ttcgtagatt ttcccatttt
     5161 cctatggtaa actgacaaag ttgagaatgt tagaacag
//
LOCUS       DASHFF010000113         5192 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.115, whole genome shotgun sequence.
ACCESSION   DASHFF010000113 DASHFF010000000
VERSION     DASHFF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.115"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            137..748
                     /locus_tag="U2I75_002712"
     CDS             137..748
                     /locus_tag="U2I75_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1697763.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="U2I75_002713"
     CDS             821..1750
                     /locus_tag="U2I75_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN1697764.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="U2I75_002714"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="U2I75_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..3871)
                     /locus_tag="U2I75_002715"
     CDS             complement(2693..3871)
                     /locus_tag="U2I75_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1697765.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEK
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3969..4067
                     /locus_tag="U2I75_002716"
     CDS             3969..4067
                     /locus_tag="U2I75_002716"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1697766.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            4098..4499
                     /locus_tag="U2I75_002717"
     CDS             4098..4499
                     /locus_tag="U2I75_002717"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1697767.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5192)
                     /locus_tag="U2I75_002718"
     CDS             complement(4706..>5192)
                     /locus_tag="U2I75_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1697768.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1642 a          878 c         1003 g         1669 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtcttct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta aa
//
LOCUS       DASHFF010000114         4955 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.116, whole genome shotgun sequence.
ACCESSION   DASHFF010000114 DASHFF010000000
VERSION     DASHFF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.116"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            209..307
                     /locus_tag="U2I75_002719"
                     /pseudo
     CDS             209..307
                     /locus_tag="U2I75_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(530..760)
                     /locus_tag="U2I75_002720"
     CDS             complement(530..760)
                     /locus_tag="U2I75_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEN1697769.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(990..1808)
                     /locus_tag="U2I75_002721"
     CDS             complement(990..1808)
                     /locus_tag="U2I75_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1697770.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1969..2658
                     /locus_tag="U2I75_002722"
     CDS             1969..2658
                     /locus_tag="U2I75_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1697771.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2672..4174
                     /locus_tag="U2I75_002723"
     CDS             2672..4174
                     /locus_tag="U2I75_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1697772.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4187..4663
                     /locus_tag="U2I75_002724"
     CDS             4187..4663
                     /locus_tag="U2I75_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1697773.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4822..>4955)
                     /locus_tag="U2I75_002725"
     CDS             complement(4822..>4955)
                     /locus_tag="U2I75_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1697774.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         1654 a          719 c          883 g         1699 t
ORIGIN      
        1 aggggcagcg agtgtcgacc ccttctaggg ggattaaaaa tacacccgtc ctcacgggtg
       61 tatgggttac tgatagactt gattttaact ggtgtcagaa acttatgtcc agaaagggtg
      121 atgaaatttt ggcaatttac ggctattcaa gaattagcac aagcaaccaa gattacaaaa
      181 cacaaattca aacattagaa aatgctggcg cagaaataat cttcctagaa aatcactgga
      241 ttatcgaaca cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt
      301 aatttgacaa ttaagagtat atttacttca gatggtaagg caacaaaata aaatacaata
      361 ggcagatgaa ctaataaata aaatctgtat taaatacaga taaattaata taaaaatcaa
      421 aaggatcata aacttttaac aagtgatgaa atgggtggaa attgattctt atagacagtt
      481 tgtctcataa cattgaagta ttttgcaatt ttagcattga aacacttttt taatcttcaa
      541 cgttgttctt ataaacaaga tgattgggaa tcttttgaat taacactgta tagcttagaa
      601 caccctttat agaaaacatt cgcttagcaa tcttaataat tccttgtgcc cgatgtcgtt
      661 tagattcagt aggttcctgc tcatcgatca tggaaaaaat ttgaaggatc agatccttaa
      721 taaatttatt taagagagga tgatccattg cttcatccat actcgataaa ctagtgataa
      781 tgagctacac gtttttcttt caagttgatc aatagcttcc acgataaaaa tatctttaga
      841 taaaatcaag aaccgtagga actacaggga tatttggtca ataagagcca ccgctattag
      901 taaaaaataa gctatcaagt atgctctcat cccaagaatt gcctacttca atcgttaaaa
      961 accgttaggt ttagctaagt gggggtagtt cactcaatac ttttatcaat aatagagcgc
     1021 aaaacttttt ctcttaattc aactgaatca atattattgt tttgttcata accatgaact
     1081 aatagatcac aaatataaag ttgtgatatt tttccagcta aagatccacc attaagaaat
     1141 tcttcaatgg ctgtttgtaa aactaagtct gcagactttc ctataggtga atgaatatag
     1201 ttagttatag caataatttt tgcgccattt tttttagcta ttttcaatga atcataagta
     1261 tctttcgttt tcccagataa agaaaaaatg attactaaat cattaactgt taataaagaa
     1321 gcgacttggg cttgaaaatg agggtctagt acagcttttg cttgaactcc tacgcgaaga
     1381 aacatatttt ctaaatcaag actagtattt ccactagatc ccactccaaa tatatatatg
     1441 ttttttgatt gagtgatcat tttaatggct tcattcaatt tttcttcgtc taaaagccta
     1501 atagtggaat agagagcttc tgttaaacta ttagcaattt catcatagta ctttacacta
     1561 gtcggatgtt cttttttttg actaaagtct tcctttgcaa tttcaatttt taaatcagaa
     1621 aaaccagaaa aaccaatttt ttgacaaaag cgaataatag tagcatcgcc aacttttgtg
     1681 tttttcttaa tatcactcat cgtactataa ataatagttt tcccagaatt taaaataaaa
     1741 tcagcgactt ttttttcaga cttcgttaaa aatggataat tttctttaat taaatagata
     1801 taactcatac aaactcctct acaatttcaa cttttctcca ttatactcca aaataataaa
     1861 aaaaactcct aaatactcca tttttatctt tacttttatg aaagcggatg ctatattgtt
     1921 tttggagtta ttatccaaaa ataataaaaa attggagcgt ttaggataat ggataaaaag
     1981 aggtttttag atcttatttc aaaaggtttg attgtttcat gtcaagcatt acctggtgaa
     2041 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
     2101 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
     2161 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
     2221 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
     2281 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
     2341 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
     2401 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
     2461 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
     2521 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
     2581 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
     2641 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
     2701 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
     2761 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
     2821 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
     2881 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggtttagc
     2941 tggtggagta gcttatttag tatatactgc aactattagt gggtttcttg aacttttttc
     3001 agcaaaagat gcaacactag atacaggtgt agtgggatca attgttgttg gtggtactgt
     3061 tgcattttta cacaatcgtt atagaaaaat tgaattacca caattcttag gtttttttgg
     3121 aggatcaaga tttattccga ttgtttctag tttagctgca atcattattg gttctttctt
     3181 ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac aaattgccca
     3241 aatgggtagt ttaggaactt ttttatatgg ttttttattg cgtttaacag gtgctgttgg
     3301 actacaccac accatttacc ctttgttctg gtatacatct ttaggtggta ccgaaactgt
     3361 agctggatca acaattgctg gagcccaaaa tattttcttt gcccaactag ctgatccaaa
     3421 ccacactggt ctgtttactt atggtactag attttttgca ggaagatttg caacaatgat
     3481 gtttgggtta ccagcagcat gttatgcaat gtatcgtgca attcctaaga aaaatcgtaa
     3541 aaagaatggt gggctttatt ttagcggggc ccttacatca tttttaactg gaattactga
     3601 accagttgaa tatatgtttt tatttgttgc tccttggctt tatgtgattc atgcattctt
     3661 agatggatta tctttttatt ttgctgatat cttaaatatt cgtgtaggga attccttttc
     3721 aggtggtttg atcgactttt tattattcgg tgtacttcaa gggaatgaca aaactaattg
     3781 gttaaaatta attccttttg gtattgcatg ggcatttata tatttctttg tttttacatt
     3841 ctttatcaaa aaatttaagg tagctattcc tggaatggaa gaagaatcag aagttcattc
     3901 tgaaaaagta ataaacaaaa atacatcatt acatgaagag tctcttatca ttattgaagc
     3961 tcttggtgga aaagaaaata ttgagtctgt tgaagcttgt gcaactcgtt tgcgtgttgc
     4021 tgttacgaat ggggagatta ttcaaaaaca aacaataaaa gacactggtg caactgctgt
     4081 cttcgaagtg aagggtggca tccaagctgt ttttggaggg aaagctgact tattaagtca
     4141 agaaatcaat caaatattag gaaaagataa ttaggaggtt tttaatatgt ttgaaatttt
     4201 caaaaagaaa aaacaagaaa ctttttatgc accttgttcc ggaaaaatca tttccatcac
     4261 agaagtaaat gatcctgtct tttcacaaaa attactaggt gatggttttg ccgtcatacc
     4321 tgatggttca aaaatttatg ctccattggc tggtcgagta acaaatatat ttcctactaa
     4381 acatgctatt aatataaaaa gcaactcagg cattgattat ttgattcata ttggaattga
     4441 tacagttgaa ttacaaggag agcctttttc gatttgtgtt caagaaaatg atgaggttac
     4501 tcctgaaacc tcgttggttg atgtggattt tgacaaaata ataaaatctg gtaaagatac
     4561 ttctgttata gttgtttttc cggagaaaaa tcagttcaat gatttaataa tagataatgg
     4621 tcgaattgct catgggaatt catgtggaaa aatcactttt taaaaaagtg tatataaaaa
     4681 gctataaaat aagctaaata ggatatttgt caaatgaagt tggtgaacag aaagaactgt
     4741 tttctttctg ttcatttttt tattttatta gtaatgattc cccatgccaa atcatgatcc
     4801 gtaatttaaa gttatgattt gtcaaattaa gctagaataa aatgcttctt gtacatagct
     4861 caaaaatatt tcgattggtg ttctgtaatt caatgatttt cttggaatat gattacgttg
     4921 attgctgaca ctggaaataa atgtctgatt cactt
//
LOCUS       DASHFF010000115         4931 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.117, whole genome shotgun sequence.
ACCESSION   DASHFF010000115 DASHFF010000000
VERSION     DASHFF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.117"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            85..972
                     /locus_tag="U2I75_002726"
     CDS             85..972
                     /locus_tag="U2I75_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1697775.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="U2I75_002727"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="U2I75_002727"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN1697776.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="U2I75_002728"
     CDS             2274..2720
                     /locus_tag="U2I75_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1697777.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="U2I75_002729"
     CDS             2993..4345
                     /locus_tag="U2I75_002729"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN1697778.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="U2I75_002730"
     CDS             4516..4803
                     /locus_tag="U2I75_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697779.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1393 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt t
//
LOCUS       DASHFF010000116         4656 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.118, whole genome shotgun sequence.
ACCESSION   DASHFF010000116 DASHFF010000000
VERSION     DASHFF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.118"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..402)
                     /locus_tag="U2I75_002731"
     CDS             complement(<1..402)
                     /locus_tag="U2I75_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN1697780.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEI"
     gene            complement(410..967)
                     /locus_tag="U2I75_002732"
     CDS             complement(410..967)
                     /locus_tag="U2I75_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1697781.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            complement(1007..1711)
                     /locus_tag="U2I75_002733"
     CDS             complement(1007..1711)
                     /locus_tag="U2I75_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN1697782.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            1897..2646
                     /locus_tag="U2I75_002734"
     CDS             1897..2646
                     /locus_tag="U2I75_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1697783.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            complement(2830..3075)
                     /locus_tag="U2I75_002735"
     CDS             complement(2830..3075)
                     /locus_tag="U2I75_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697784.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            3395..4156
                     /locus_tag="U2I75_002736"
     CDS             3395..4156
                     /locus_tag="U2I75_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN1697785.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            4384..4578
                     /locus_tag="U2I75_002737"
     CDS             4384..4578
                     /locus_tag="U2I75_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697786.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT         1500 a          846 c          727 g         1583 t
ORIGIN      
        1 aatttctttt ttcaacacat cacttgcatt tatcacattt tcattagcta cgatattgat
       61 gctatgcttt tgttcaaatc gcttacttaa ggcttgcgca atgatcggtg caatttttac
      121 taaatttatt gcgccaacag atgttgttaa gatatctgca ttttttgttg ctgtaatcac
      181 ctcttctagc tcggataaac tattcagtgc cacaacatga tcaatgaaat cactcctgtt
      241 catttcatca ataatttcga tagtataacc cttatcttga tttaattgat ggatcagttc
      301 aggattcgta tcaataaaac atagatcgaa tccattctga tgtagaattt gtccaataag
      361 ccctctgccg atgttccctg ctccaaaatg taccgctttc atttttcacc tactctaagt
      421 tagcatggta ttgccacaac tttgacatat caattgcttt ttcttctatc atcactaaag
      481 caatactatc tcctaacaaa agaacacttt gttcaaacag gctagtcatt ggctgtatag
      541 aagaaatttc ctctggtaac gctaattttg tttgtacggg tatacgaata aaaagatctg
      601 aatattcttt cattgtgctg tctgggtttg atcctatatg ggcaacttgg gacccaaatt
      661 cctttgcttt ttttgcaata gttagtggga aagcactttc cccactacca gaaccaacaa
      721 tcaataaatc tttatctgta attgccggtt cagtgatttg tcctacgact actgtctgaa
      781 ttcctagatg ccctaagcgt tttgccattc cttctagcgc taacagtact ctgccaaccc
      841 ccacgaaaaa aactcgttcc gctttttcaa ttttctttat caattgttcc acatcttttg
      901 aagaaatacg gtctaatgtt tcagtaattt ctcttacaat attttgtttc gccgccttat
      961 aactcaacta tttcccctcc atttctatta ttcaggacca ctacatctat tgatttttcc
     1021 taactgattg cttcatcgct tgataaagta gtttttgatt ttctgctaat gtatttcctt
     1081 taagaaacaa acttgtactt cctaatacca gatttgttgc gcctgcatct aataatcccg
     1141 ggatcgtttc taacgatacc cgtccatcca cttggatatc tactgctaac tctttctcat
     1201 ttactaactg gcaaagatct gtaatttttt ttagcgcgta agaaacttgt ttttctgact
     1261 tgtttgttgc aaaacctgga ttaatcagca tcaaacaaat cctatcaatc tcgggtaaaa
     1321 tatattgtaa tacatttaac ggagttgctg gattcaaagc tatccctact tttgttccag
     1381 aattcttgat gagctggata tatcgatcaa tatggaggct tgtttcataa tgaaaagtaa
     1441 tgctctctac gccaattttc aacatttctc caataaaaaa ttcgttatcg ttatccttag
     1501 ccatgatgtg tacatcaaaa ttcattgaag tgatttctct aattcttttg attgtttcga
     1561 tccctaaagg catactggga ctaaaagaac cgtcaatcac gtcaatatgc aaggaatcca
     1621 tcccaatact ttctagttct tggatgcttc cttccaaatt tgctagatcc ccacacataa
     1681 gtgaaggcga caaaataatt tttttctcca tacattccct cctttaatta ccccctcatt
     1741 gtaaccgctt aacaaatact agtcaacata aaactgcata aaaatatttt gtttttgctt
     1801 gtttatgcct ttttagtgta ttaaaaacgg aaattattga cataaaaacg cataaatgac
     1861 atgataaaca agaataaata agaaggagca cgcaacatgt tgaaattaga acgtcatcaa
     1921 aaaattctag atttattaaa cgcagaacaa aaagttatcg ctagtgattt aagtcaacgt
     1981 ttcaatgtat ccgaagacac gattcgacga gatttgaaag atttggatag ccaaggtctc
     2041 ttaaaaagag tacacagcgg cgctttacgt gttggtccgc caattaccaa ttttaactac
     2101 cgagaaactg tagctagcga acaaaaaact gcattagctc ataaagccct ccctttttta
     2161 aaagaagaat ctgtcatcat cattgatgga agtacaacta acttggcttt agcaaaagaa
     2221 atcccacaag attttcgtgc aacaatcatt accaatagcc cgccgattgc catagcatta
     2281 gcacagcatc ctaatttgga aattatcaat attggcggta ctttttataa acaatctatg
     2341 atcaatattg gggtacaagc ttataagcaa ctacaaaaca ttcgagccga cctttatatt
     2401 atgggtattt ataacataga tctagatgtt ggaacgagcg tgccaactat gcaagaatcc
     2461 gacattaaac gtgagatgat acgtatttct actgaagtac ttgggatgat aactaatgat
     2521 aaattcggca ccatcagtaa tcatatcgtc ggtccaattg aagaccttac ctatcttatt
     2581 tcagaagaaa ttcctgaaaa ggttcgtaaa gaatacaata aacaaaaaat cgttttgatt
     2641 gattaagcga aaactattgg taactactaa agcaacagat ataaaaaatc taaaaaagaa
     2701 acacgcaata aagttttgaa ataaacttta ttgcgtgttt ccttttttgt ttattctcac
     2761 ttacctagca ccacgtataa catactatag caatcatcat cccacttctt tgtcttttga
     2821 aatactacgt tatttctttt tattaacaaa aaacaatagc actaaacaga ccaaaatagt
     2881 tatgactctc aacgcgggag taaacagcaa tgctatcatt aaattagcaa gttcactgac
     2941 gattgaggaa tttgattcgt ataatacaaa taaaggttct gcaaataaat tcacatgtct
     3001 ataaacaaat tctgtaaata aatatacggt taagcctaaa ccaattatgg tgatattttt
     3061 tttagatttt gacatttttt ctctcctata ataatttttt cagttattca tttccttttt
     3121 tataaaaaat gtagattgtt ttgaagtaat aatatgctgt agtttcaaga aaatttacag
     3181 gaaatttgat aactgatatt atttactttt tgaccaccat atacatgact tttatttttg
     3241 tttctttttt agaatcaaat gtgattaaat agaaaatttt acaataatat gcacatataa
     3301 ataataagat gatataatca acaaagaaaa tgtgattttt ttcactatat tttatcacat
     3361 gaaaattcat taagaaagta ggaatcgaag tattatgcac acaccagaag aaattatgga
     3421 actcagtatt catcttggaa caaaaaaaat acataaacct atcttagcta aactgatttt
     3481 aggttttatc ggaggagcca tgatttctct aggttatttg gcatacgtac gagtctctgc
     3541 ttctatccca gaatctatgg ctagcttgac tagtttagtt ggtgcctcgg tttttcctat
     3601 cggcctgctg gtcatccttc ttggcggtgg tgaattgatc accggaaata tgatggctgt
     3661 ggcgattgcc tttatggata aaaaagtcac attctcacag ctggttaaga attggtcagt
     3721 gattactttt gcaaatatta ttggtgcaat tttcgttgcc tatttctttg gacactttgt
     3781 tggtttaact cattcaggtg tctatctcga ccaagtgatt catattgctg atcataaaat
     3841 cgctgtttct tggtggcaag cattaggctc cggaataggt tgtaattggt ttgtcggttt
     3901 agcgttatgg ttatcatatg gagcaaaaga tgcggccggc aaagtactaa cgatttggtt
     3961 tccagtcatg atttttgtgg caatcggttt ccagcatagt gtggccaact gttttgtgat
     4021 acctgctgcc atatttgaag gtcaagcgac ttggttagaa tttctctcga attttatccc
     4081 cgtttatatt ggaaatatca tcggcggagc tatttttgtt tcaggttttt attacgttag
     4141 ttataagcat cattaagatt gaaggtaatg ttcattcgag cattaccttc aatctcttta
     4201 tatactattt atattttaag aacaatagcc ttttattata tatattattc aaattagtta
     4261 tatcgagtga aaaaaacttt caaattgatt ctttttattt atcataaaga tatagataca
     4321 taatcatttt cacttacata tatttgaata ttaactaaaa acagcaagaa agaagaggat
     4381 caaatgtatt atgtagaagt aaaaacaaaa ggcgtcaaaa acaaacaata tgtgaaaggt
     4441 atcagtaacg aatatccttt acttggttca tggaaagaag ctgctccttt ttccaaacca
     4501 tgtgccataa aaataaagaa cgagctagaa aaagaactga cttgtggcaa agctgttgta
     4561 gatatcatcg aaaagtaaat ccaatgtata gaaaggaaga tcacacatga aaagcgacgt
     4621 acaaattgct aagattatag aattagaaga agaaga
//
LOCUS       DASHFF010000117         4587 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.119, whole genome shotgun sequence.
ACCESSION   DASHFF010000117 DASHFF010000000
VERSION     DASHFF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.119"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            38..1255
                     /locus_tag="U2I75_002738"
     CDS             38..1255
                     /locus_tag="U2I75_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN1697787.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            1269..4418
                     /locus_tag="U2I75_002739"
     CDS             1269..4418
                     /locus_tag="U2I75_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN1697788.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1616 a          751 c          969 g         1251 t
ORIGIN      
        1 gaagcgtggt tacaaggtgc gctggaggtg ttcaaacatg agcaatgata cacgaccaga
       61 aatccggttt cctggtttta cggaagattg ggaagagcgt aagttaggag atatgatgga
      121 tgttacttct gtgaagcgaa ttcatcaatc agattggact aactcaggta ttagattttt
      181 acgtgctcgt gatattgtct cagctgcaaa aaatgaggaa ccatccgatt atctttacat
      241 ttcagaagaa aaatataatg aatattcgaa aaattcaggt aaagtttctc aaggagactt
      301 gcttgtaact ggtgtcggat caataggtgt ccccttatta atcactgatg ataacccaat
      361 ttactttaag gatggaaaca tcatttggtt caagaatgag cataaaattg acggaaactt
      421 cttttactac tcgttcataa ataacaaaat tcagaagtat atccgtgacg ttgcaggcat
      481 tggaaccgtc ggaacctata caattgatag tggtaaaaag acacctatct cacttccaac
      541 ttatgacgaa cagatcaaga tcggttcatt tttcaaacaa cttgatcaca ccatcatgct
      601 tcaccagcac gaattagatt tgctgaagga aacgaaaaaa ggattcttac aaaaaatgtt
      661 ccctaaaaac ggagcaaaag tgccagaagt tcgttttcca ggatttacgg gagattggga
      721 agagcggaag ttgggggaag tggtagtttt tttgaatggt agagctttta aacaacaaga
      781 attacttgat tctggaaaat atagggtttt aagagtaggt aattttaata ctaatgatag
      841 atggtattat tcaaacttag aattagagga agacaagtat gctaataaag gagatctctt
      901 atatttatgg gcaacaagct tcggtcctga aatgtggaat gaagaacgtg taatctacca
      961 ttatcatatt tggaaactta aatttttatc taaggaattg gacaaacagt atctatatac
     1021 ttggctattg actgataaag agaaaattaa agaatcaact aacggaacga caatggtaca
     1081 tgtgactaaa ggtggtatag aacaaaggac atttcagttt ccaccggata taaatgaaca
     1141 acaaaaaatt ggtgccttct tcaagcagct agatgatacg atcgctcttc atcaacgtaa
     1201 gttagatttg ctgaaggaaa cgaaaaaagg cttcttacaa aagatgtttg tttagggagg
     1261 gaagataaat gaaaattagt caccgtgagg aagcggaagt cgaagaacag ttgatccgtg
     1321 tattggggga agggcataat cagtggacgt atcgtcccga tctaaaatca gaagaagatc
     1381 tttgggtcaa tttacgccaa aaaatcatct caaataatca agcagaactg aatgattttc
     1441 ctttgaccga taaagaattt gaaacgatca aaacagaatt gttactacga acgaaaaccc
     1501 cctttgatgc tgcgaaatgg ttaaaaggtg aaaatggaat ggctcggatc acgattgaac
     1561 gggaagatcc gcaactgggt tctgtctcgc tcattcttta ttctaatcaa gacattggtg
     1621 gaggaatctc tacctatgaa gtggtccatc aaattgcaaa acgtggaagt aacatagaag
     1681 cacgggatcg acgttttgac gtcaccttac tgatcaatgg attaccgatt gtccaaatcg
     1741 agctgaaaca agtaaccgca aaagatagtt tttatcaggc gtttaaccaa atcaaaaaat
     1801 atgcagaaga aggtatgttt cggaacaata tcttttcgac cttacagttg tttgtggttt
     1861 ccaatgaaca aaccactcgg tattttgcaa atgccttacc aaaggatttg catcctaagt
     1921 ttttatttag ttggcgaacg aaagataata aaaaggtaga aaacttatat gagttttgca
     1981 aacaagtgct aaatatcccg gatgctcatc gcttgattgc ggattatacg attgtcagtg
     2041 aagatcaaga caataaaatc ctcatggtgt tacatcctta tcaagtacat gcgattcaag
     2101 ccttgtttat tgctgcaaat aaacatcaat caggatatgt gtggcatgcg actggttcag
     2161 gaaaaacgtt gactagtttt gtatctacta agcttttagc acgaaaatcg gggatcgatc
     2221 ggacgatcat gttagtggac cgcaaagatt tagataatca aaccaccaca gagtttacga
     2281 aatttgcttc ggaatttaat actgggattt cttccgggaa cgccaaagcg aatagcttga
     2341 tcgtgggaac aggaagtgcc aaagagttaa gtgaaactct gttggcagat gccaatgcaa
     2401 atgttgtgat tattacaacc cgtcaaaagc tagatgcggc gttgaaatat gcaaaaaaac
     2461 aagaagagaa aaaaggaacc aatcgcttcc aaaaattaat ggggcaacat atcgtgtttg
     2521 tagtagatga gtgtcatcga gcgttaagtg ctgaaaatat ggaagaaatc aaaaaaatgt
     2581 ttccgaaatc cacttggttt ggctttacag gtacaccgat tttccctgag aatcggaaac
     2641 aagccaaagg gcaattggca cgtacgaccc atgatcagta tggagaggtc ttgcacacct
     2701 ataccatcaa gaatgcatta gaagatggct cggttttagg atttcaagta gagcatgaaa
     2761 acaccgtgga gccgacttca ttagagaata aaatctatcg gaaattaaaa gaagtagaaa
     2821 catatgcaga gtattcaccg gaacagatca atcgtatgat cgatcaaatg gagtctgtca
     2881 aaaaagaaag ttatttggac cctactgttt ttgaagcaga ggaacatatc caaaaagtca
     2941 ttcataaaat gttccgtcca gacaatgcct atacaaaatt tgattttcga aatggccgtc
     3001 caacgaagtc tgcgatctta accacgagtt ccattgatat ggccaaaaag tattatcggg
     3061 ccatcaaaga gatgacgaaa gaatctgatt ggttaataaa agagtttccc aatcagccta
     3121 tccgtgaagg acgaacgatg gaggatcctg atttcccacg aatcgctatt acctattcat
     3181 tagaagaaaa tagccgagat tcaagtgccc aacaagaaga aatgcaaaaa attattgagg
     3241 aatataatca gtattatgat actgcctggt cactagcaga cattgagcgt tataacggag
     3301 acatcaataa tcgtttggca cgaaaaagag cggaattcaa acaatttggg aaacaaatcg
     3361 atttagtaat cgtagtggat cgtctattga caggttttga cgcccctacg attcaaaccc
     3421 tatttgtgga tcgtaatcta gagtatgcag gattgatcca agccttttca cgtacgaacc
     3481 gtacgtatcc tgaaaaaaca aaaggactaa ttgttacttt ccgaaagcca gcgacaatgg
     3541 agaagaatgt cgaggatgcg acaaaactgt attcagaagc taaagaagaa tcgggtttga
     3601 tttatccaag ttaccaagaa tccaaaaaaa gattcaagca agcatatggt aagttaaaag
     3661 aggtttcatc catggaatcc attgatgaac atacgccact tgaaatccgt atcgagtacg
     3721 tcaaagcatt tcaagaattg aatcgtgcgt atgaatcgtt agtgacttat gatgattaca
     3781 atgatgacat ggtgcaatct tcaactttga acaatcaaat tcaacttttg gaagaacaaa
     3841 ttggcgtgta cgagaccgtg aagggttcat tgatggagga agaaccagaa aaagagggag
     3901 aagatttttc aacgatcgaa ttttatagtg aaaataccac gaagttgtat gatattgatt
     3961 ctacttatat tgatcaatta ctaggaactt atgctgcgaa tagttcagat gcacgagaag
     4021 aaattgaaaa agctttggct aaattgaata aaacagaagg agtcaaagaa gtttaccgac
     4081 aaattttaaa tgcatttgat gtggggactt tagatagtaa tgaagatatc tttgtcatta
     4141 aacgtcgcta ttttacacaa gcaagtgata atttgattca tgccttttcc aatgagtggt
     4201 ttgtttctga gaaagaatta catgcttcta atctgcaata tatgccaggt gaagatccaa
     4261 ttcctaatat gaaagcaatt atcaacagta aggattacga aggatataaa gccaaacatc
     4321 cagaagcgaa accatttaaa tacccacaag aaatgaagcg agcatggcgt aaaatgttag
     4381 atgatgagtt gattccactt gaaaatgaat tgaggtaaac gagaagagtt aagaaaattg
     4441 tacgattcaa agatagtagt aaagtggaag tgtagaaaat agggatagat gtctttttac
     4501 ttgttcacat tttataacgg agtatttaag aaagctattg aataatgatt cttccatctt
     4561 tttcattcat ttaccggttc tgttgca
//
LOCUS       DASHFF010000118         4474 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.120, whole genome shotgun sequence.
ACCESSION   DASHFF010000118 DASHFF010000000
VERSION     DASHFF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.120"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            320..1051
                     /locus_tag="U2I75_002740"
     CDS             320..1051
                     /locus_tag="U2I75_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1697789.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYQFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1196..3223
                     /locus_tag="U2I75_002741"
     CDS             1196..3223
                     /locus_tag="U2I75_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN1697790.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3331..4428
                     /locus_tag="U2I75_002742"
     CDS             3331..4428
                     /locus_tag="U2I75_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1697791.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          924 g         1261 t
ORIGIN      
        1 aaatataata tacatttttt atttaattat aattaatatt gattgaagtc taggcagtca
       61 agtcacattt tgatagatag gagggggact tacgaattat tttaaaggct atgacagctg
      121 tatttctaga ttgtgtttgc atcaagttgc aataaaaact agaaatcaaa tcgggctgat
      181 aatcatttat aactaatcga tacataggac caaatatagg tctatgttct tttatttcat
      241 aagtatcgta aaacagaatt ttgctatgga ttaatccaat cgactgatat gtttagtaaa
      301 taaagaaaga ggaatgtcaa tgatgaaaaa agaaatcgta ttaacagcag caatgatgtt
      361 attcagcatg gtagcaagta cgacttttgt ttctgcaact gaagtatacc caaaggaata
      421 taacacagaa ggaacgatta catttgaagc aggagatgag ggtgtaactc ctcctgttga
      481 tccagagaat cctgatccca ataaacctgt cgatccatca gatcctccaa gccctggaac
      541 aggaggggca ttatcgattg attatggatc aaaattcaaa tttgggactc aaaaaatttc
      601 tactgcggat aaaacgtatt atgcagctgc tgatgtgatg aacgatgggt ctagaaaacc
      661 gacatatgtc caagtgacag ataggagaag taccttatct gggtggaaat taagtgtctc
      721 tcaaccagaa cagtttaaaa ctgccagtgg ggatgaacta gttggagcac agctgaaatt
      781 tacgaaaggg caggctgttt ctttggttga tccaacatat acgccgcaaa cggtgaattc
      841 tgaattaacg ttgacaccgg gaggaaacaa tacgttagca attaatgcta aatcaggaac
      901 aggtgtgggg acttgggtct atcaatttgg tgcaaatgaa aatgaaaatc aagatgcggt
      961 acagctttcg gtacccggta aatctgtcaa gttagcgcaa caatattcca ctaaattagt
     1021 ttggacatta gaagatacac ctaacaacta gtttactgaa aagaactgtt aaaaataaat
     1081 acgtagtcat attgtcttgc gaggtaatcg agcaaaaatt gtcaaaggct aggctaaatt
     1141 tgtttggata cgaatagttc atctaggaat ttaacgatca ggaggaaaag atatattgaa
     1201 aaagaggagg atcgtgctca ttttaagcag tctcttagtg actaatatct ttccgatcat
     1261 agggtactct ttggaattga atcagttgga gcatggaaat agaagagacg aagaaagcaa
     1321 actcacttcg acattcgaca gtttaataga atccaaccag acacaagaaa gcagtgagga
     1381 tttaagtatt tcgagtagtg gagagaccac tgacaacgta gaagctgagg gttcggacaa
     1441 acaaggacta gaagatgcac caatttctgc ggttgagctg cctgacgatc ccaatattat
     1501 ttctatcgat aaaatttttc aaacacctat cggtactagc acgtccatat tggaaaatgg
     1561 gaagttactg cagttgaacc cagcagaaag gtctcaaaga ggagctattt ggtcaaataa
     1621 gaagattagt ttactctcgg attttacatt taaatcttat ttatacttag gaaatcaacg
     1681 agatagagcg ggagatggca tgacctttac attgactggc gatcctagaa tggctagtaa
     1741 tcccagtcaa gttattggat cgccaggtat gggaattggc tcatattcta ctcgttctgg
     1801 ccaaccttac attgcgaatg cgctctcgat tgagtttgat acgtataaaa atagtggttc
     1861 ttctgatcga atggatcgag agataactgc tgataataga gggcatggac atgtcgcttt
     1921 tgtcacgcct aaaccaaata ataataatta ttctggagag catactggcg tgacggtagc
     1981 tcctacattt ctttctaatg gcacatggcg cttgttaata attaaatggg acgcggagca
     2041 aagaagtctt acttatgatt tggaaggtgt cggtcactct tctcggatta tacctgatct
     2101 cattaggcag tttggttcta cagaagtaaa ttgggggttt acttcttcaa caggtacttt
     2161 ttttcaagaa aatgctgtcg ctgtcacgcg acttccatcg aatgtcacat caacagctga
     2221 tgtaaaagtc aacgatagtg attatggggc catcgctgaa gctggaggaa acgataagat
     2281 aactatcaga aatacattga aggtcgaggg ggattttctt gaatataaga aaatgcagac
     2341 tagtatcgac ctcccacaag aattaactat tcccgagcat atcttgatca atggaaatca
     2401 aattgatgcc aaagatatct tgataaccgg aaatcgagtc acgctaaatc tgacagatta
     2461 ttttattgct ggagatacgt taataatcga attggagaca actttgcagg aagtaattcc
     2521 tgaaaaagta ctcactatgc attttgaata ccttgaaaac gggaatttga ttgagcaatc
     2581 gaatgaaata aacctaaaga tcgcaaagct gaaggagata gatatccatg tttattataa
     2641 agataaagac agtcagattg atattgctga aatgaaaaca atcactggga aaataggaga
     2701 gaagtataaa gaaactccta ttgatattga agggtatgtt tttgactcgg attcagcgaa
     2761 tacagaaggc atcttctccg aaaatacaga ggatatctat ttttattaca gaaaaggcaa
     2821 actttatctc tcaactgctc ctcaatatgt caattttgga aaacataaaa tatccagtag
     2881 accattaagt gaattcggac agttttcagg tgggttgaaa attatagatg aacgggcttt
     2941 tggaagttgg aaattacaat tgaaacaaga gaaagttctt acaaatggag attttgagct
     3001 tccagaagct ctttcctttg ttacctctga aaatacaacg gttataggga catccgctgt
     3061 tacgattttc gaaagtgatc aaaagggtga aagcgatttg tcctctcttt tagattctac
     3121 agctcatcgt ggaatcagaa tagatattcc tgtagaaaac caaagaatcg gaacctttga
     3181 ggggaaacta tcttggatac ttgcagatgt cccaggaaat taacaggaag agaagaatat
     3241 aacaaaatta ttgagtttca agaagggcac aggtaccata ggctcttgtg tcccacagag
     3301 acaagatttt gttatagtct tctcttacat atgattaaag gagcgatttt cttgagacca
     3361 aaacaacaat tatttataac actcctagtt attttagggg cactttttaa caatatatca
     3421 gtgtcagcta gtgcatttaa tttttcagtg acgccgataa cttcagagaa tcaaatcgac
     3481 aagagaaaaa cttacttcga tcttcaactt gatccggatc aggaagtaga agtaaaagcg
     3541 gaattgagaa atgatacaga aaaagaagtg aaaatcgata tttctgtgaa tagcgcaacc
     3601 accaattcaa atgtgatggt tgaatacgga aaaaacgaga ttgaaaaaga catgagcctg
     3661 atctttgatc tggtagatta tgtgagctat ccacagatag tgacgctaaa acctaaaagt
     3721 gtccaaaccg ttgcttttca tgtcaggatg ccgaatgagc gatttgatgg tgtattggct
     3781 ggtgggatca cattccaaga aatccagacg gaaaaggacc aaacagaaac taaggatcaa
     3841 agcttatcga ttgagaatga atacacctat attattgctt tgttgatgca acagacactg
     3901 aacgaagttg ctccgaacct tactcttcat gaagttaaac cagatcaaat caatgcaaga
     3961 aatgtcatct tagcaaacgt tcaaaatgat caaaaaacct atatcaatca agtagttatt
     4021 gaaacaaaaa ttacaaaaaa aggacattct gaagttttat atcaagagga aaaagaagga
     4081 ttgcagattg cgccgaatac aaatttttct tttccaactg ctttaaatgg tcaaccgcta
     4141 accccaggtg aatatcacct tacaatgact gttttaggaa atgaaaatga aaatggaaaa
     4201 ttttcgagaa aaaaaggaaa tactacgatc aactatacca atcaatgggt atttgaaaaa
     4261 gattttacaa tagacggaaa agtagccaag gaattgaaca cgacggatgt tacgattaaa
     4321 aaagacaata attggattta tatcttagta gcttcacttt tcttattgtt tattttatta
     4381 tgcatacttt tgtttgtttt gagaaaaaaa gaagagaaag aaaagtaagg ttaagataac
     4441 aaatagtaaa tttttattat tgtaggatgg ttgg
//
LOCUS       DASHFF010000119         4461 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.121, whole genome shotgun sequence.
ACCESSION   DASHFF010000119 DASHFF010000000
VERSION     DASHFF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.121"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            574..1008
                     /locus_tag="U2I75_002743"
     CDS             574..1008
                     /locus_tag="U2I75_002743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697792.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="U2I75_002744"
     CDS             2298..3221
                     /locus_tag="U2I75_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1697793.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="U2I75_002745"
     CDS             3237..3776
                     /locus_tag="U2I75_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1697794.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DASHFF010000120         4424 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.122, whole genome shotgun sequence.
ACCESSION   DASHFF010000120 DASHFF010000000
VERSION     DASHFF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.122"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            690..1658
                     /locus_tag="U2I75_002746"
     CDS             690..1658
                     /locus_tag="U2I75_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1697795.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="U2I75_002747"
     CDS             1669..2484
                     /locus_tag="U2I75_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEN1697796.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="U2I75_002748"
     CDS             2552..3262
                     /locus_tag="U2I75_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1697797.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4424
                     /locus_tag="U2I75_002749"
     CDS             3298..>4424
                     /locus_tag="U2I75_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN1697798.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1490 a          661 c          921 g         1352 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaa
//
LOCUS       DASHFF010000121         4416 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.123, whole genome shotgun sequence.
ACCESSION   DASHFF010000121 DASHFF010000000
VERSION     DASHFF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.123"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            59..913
                     /locus_tag="U2I75_002750"
     CDS             59..913
                     /locus_tag="U2I75_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1697799.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="U2I75_002751"
     CDS             1010..3259
                     /locus_tag="U2I75_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1697800.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     NWVITQEEK"
     gene            3262..>4416
                     /locus_tag="U2I75_002752"
     CDS             3262..>4416
                     /locus_tag="U2I75_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1697801.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1557 a          625 c          806 g         1428 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga attgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DASHFF010000122         4408 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.124, whole genome shotgun sequence.
ACCESSION   DASHFF010000122 DASHFF010000000
VERSION     DASHFF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.124"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(205..660)
                     /locus_tag="U2I75_002753"
     CDS             complement(205..660)
                     /locus_tag="U2I75_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697802.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1068..1238)
                     /locus_tag="U2I75_002754"
     CDS             complement(1068..1238)
                     /locus_tag="U2I75_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697803.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(1254..1724)
                     /locus_tag="U2I75_002755"
     CDS             complement(1254..1724)
                     /locus_tag="U2I75_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1697804.1"
                     /translation="MTDKSIKEFAEELGVSKQAIRKYFDKLPTKLTPKLIGGKYVISA
                     KAQEFIKNSINQINEVDTEVDSLVDSILIAELKEKNKQIDHLQGVIEIQQKLLDQQQQ
                     LTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIEKQTKKKQKWWKLWK"
     gene            complement(1734..2702)
                     /locus_tag="U2I75_002756"
     CDS             complement(1734..2702)
                     /locus_tag="U2I75_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1697805.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNRALELANRQTQHEKETTSLQERIKEQKRKLELL
                     QQLEELEKQQKNRENETDKQKLDNNSDIPLELRKDLLDNLQNLFKD"
     gene            complement(3861..4025)
                     /locus_tag="U2I75_002757"
     CDS             complement(3861..4025)
                     /locus_tag="U2I75_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697806.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKCSST"
     gene            complement(4044..>4408)
                     /locus_tag="U2I75_002758"
     CDS             complement(4044..>4408)
                     /locus_tag="U2I75_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1697807.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1358 a          734 c          703 g         1613 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa cgtgttattt taaaggttag
      781 tagacataat gtcgactatg ccaagtagcc cttgtatcaa ccgatacaga ggctcacttt
      841 gtgattttgg attggtctag gtaaaaagct ctttttaagg cttgattttt cgcggttatc
      901 tatatctaat tctttgtgca tttttatctg tcatttttta aagacttgtt atttttgaga
      961 ggatttgata cgctttgttt gtatctcatt ttcatctaat aaatggttta tgttgctaaa
     1021 ctaggataat ctcattttgg gctgctcgtt agagcagtct ttttttatta tttatccgtt
     1081 tcttttacaa ataactcttc gttaggactg ttgtaataat ttggattcaa tttcaattca
     1141 ccatgtttat ttcctttatt gatcactgac aataacatcg gactgaagcc tagaattcct
     1201 agtaaaatag aaccaaatat gagctttttt cttgtcatga atgtttcctc cagttatttc
     1261 catagtttcc accatttttg cttcttcttt gtttgttttt ctatcgattc tgtttcttca
     1321 gaaagagtag ttgattcatt ttctgaagtt tcttccgtat aaactaattg taaccgtttt
     1381 tgtagtttgt cgatttgttg atttgcctgc aaggtgagtt gttgttgctg gtcgagtaat
     1441 ttttgttgta tttctatgac cccttgtaaa tgatcaattt gtttgttttt ttcttttaat
     1501 tctgcaatta aaatactatc aactaaactg tcaacttcag tatcaacttc atttatttgg
     1561 ttgatactat tttttataaa ctcttgagct tttgctgata ttacgtattt tccacctatc
     1621 aacttaggtg tcaacttggt tggcagttta tcaaaatact ttctaatggc ttgtttgctg
     1681 acacctagtt cttctgcaaa ttcttttatt gatttatctg tcatgaatac tccttagtct
     1741 ttaaataagt tttgaagatt atcaagtaag tcttttctta gttctagagg aatatcggaa
     1801 ttgttgtcta acttttgttt atccgtttcg ttttctctat ttttttgttg tttttctaac
     1861 tcttctaact gttgtaaaag ttctaatttt cgcttttgtt ccttaattct ttcttgtaat
     1921 gaagtagttt ctttttcgtg ttgagtttgt cgattagcta gttctaaagc ccgattttgt
     1981 tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt ttttgatttc tggtttaaat
     2041 gtgaattggt agccaatgac aggcttccca cggccttttc cgtatttctt tttgattgtt
     2101 aaacctttaa agattgcggt tagttcttgt cgtattggat tgaacacacg ttgatcgatc
     2161 gtaccttggt tgtaactttt aggaatatca agtaattcat gaaagtcttc ttttgaaaaa
     2221 taagcgaaac ctttggttct gtattgtttt agtaatcgaa acattgtttt tgaataactg
     2281 ctttctaatt cattgaattg ttggagagaa tagcgcaccc attcttcaag attattaagt
     2341 aagggtaagg ctttttcatg aacttgtatt tcagcaaaag gaatatcagc ttttccgtcg
     2401 attttaaatt gattaaataa aacaaaacgt tcacgttgca aaccgtcagc acttcttcta
     2461 ccaaaacgta aatccattaa tttgtcatac gttgtttcta aatcatctat gaatcgaaca
     2521 tttgcagttg ctttataatt gcttaaatct tttaattgct cgaatgtaaa ttgtactttt
     2581 ttatcaccta catcacgcat tcttgatacg atagagaaga acaaattcat ttcgactggt
     2641 gtaaattttc gcaacggaat tgtatttaat tcatgatgat actttacgat ttcattagac
     2701 atatttaaaa tagctccttt cttactcgca caagagatac agacgttatg aaatacaaaa
     2761 tgtctgttta tctacatctt atattaacat cgtcgatgta ctaattcaat gtacaaacag
     2821 acaaaacgat gtagataaac agacaaaacg atgtagataa acagacaaaa cgatgtagat
     2881 aaacagacaa aacgatgtag ataaaagcgt cattcccttg ggagagtaag gatacagccg
     2941 acggtaaaga ggtttaaaaa gaggtttaaa aagagattat aaaagagggg gacaaaattt
     3001 cccgcctaaa aaaccacttc ttttttctcc aaacagattc gcgcattttt ctaaactggg
     3061 aatcttactc cctatagttg cttaaaaagg tgtgatcgct ctgctctcac attatactgc
     3121 gcagtcgcct ccggctcctt tgctaaaact gttcataagg aactctttga gaagctatga
     3181 gccttcgttt gagacgtttt aagacagtct tagtataaat cccttatctt gttcttaaag
     3241 cgccttacag gcggtcaggg caagccctaa caaccctaaa attgtctgaa ttccctctaa
     3301 aattcgattt aagcactctt aactctattc ttatatattt atatacttag acatttgaaa
     3361 cgccttaaaa cgcaaattag agcttgataa agatatttgc tcgtgtgcct agacttttaa
     3421 aaaagtcggc acacgccatg acaaagctga aataaactgt ttcagctttg tcatggcgga
     3481 ttcgctcact tgaaaaagtg ttcatttcgc tcgattatta gcatatattt tttcgctttt
     3541 ttgctagcca aattttgcat tagttcgctc ataagtccca ccctttcagt acgtccgagg
     3601 gacgtatcgt tcgtaatgtg agagtgccta taaggtcact ctctacaaaa ctcactaaaa
     3661 gggtttggca agctctatat ttgctagcca aatacctacg tatttggtgc aaataaccgc
     3721 ttgtcaaagg ggcgcaagct cccccttgaa tcccccctca aaacccacgc tccacaatac
     3781 aaaaaaggct agcaaatgct agccactttt tgtaaagttt cgcttgtttc accttcggtg
     3841 aaattgttgc tttgcaacga tcaggtagag ctacattttt ttcgttgttc aaatattctt
     3901 acgccattta ctattaaaat cgtaggtatt ccaaagtaaa gaaaaaattg attagcaggt
     3961 aatacaaaat ataaaataat accagcaata attaccaaac ttatagtaag ttttgttaat
     4021 ttcataaagt atgccacctt atcttatata gatatcaaat agattaatat gccgattgta
     4081 ttataaaatc catgagcaac tatggaatac catatatttt ttgtttgtat ataaacaaaa
     4141 cttaaaataa taccaataaa gaaaatactt aggcctcccc catgaacaat agaaaacaga
     4201 aaagaagaaa ttagtattga aatatattgt tgttttttgt ttgcttgagg atcaatcata
     4261 caaaatcgaa aaattccttc ttctaaaaaa gctccataag tagcagcatt taaaaagaat
     4321 aaaaggagag aaccatttac ttcattagga tttatacctt ctgaaaaaga tgtatttaga
     4381 atattgatta ggaataaaac agagaatg
//
LOCUS       DASHFF010000123         4362 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.125, whole genome shotgun sequence.
ACCESSION   DASHFF010000123 DASHFF010000000
VERSION     DASHFF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.125"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(73..693)
                     /locus_tag="U2I75_002759"
     CDS             complement(73..693)
                     /locus_tag="U2I75_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN1697808.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(769..1206)
                     /locus_tag="U2I75_002760"
     CDS             complement(769..1206)
                     /locus_tag="U2I75_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN1697809.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1369..2199
                     /locus_tag="U2I75_002761"
     CDS             1369..2199
                     /locus_tag="U2I75_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN1697810.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2183..3550
                     /locus_tag="U2I75_002762"
     CDS             2183..3550
                     /locus_tag="U2I75_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1697811.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3569..>4362
                     /gene="yidA"
                     /locus_tag="U2I75_002763"
     CDS             3569..>4362
                     /gene="yidA"
                     /locus_tag="U2I75_002763"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN1697812.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAI"
BASE COUNT         1323 a          920 c          803 g         1316 t
ORIGIN      
        1 aaaatcgtgt cacacctcct tattctgata ggctgatcgt ctctgtcaca acctctcgta
       61 agaaaataat gtttattctt tttcagagtc agaaaattct tcctcttcat aatcttcatc
      121 atcatcgtct tcgtctatga tacttaaatc ttcttcgata tttcctggca agtcttcttc
      181 atcatctgtc gggtcatctg ctccgatttc ttgcaagtca gaattataag cagcgatttc
      241 ttcatcttca tcatcatcgt catcaaacag atcatcgtca tcttcttctg tcagatctgt
      301 atcttctggg tcatcgtcat tgtaatcgat cgcgtcttca tcatcattga taaaggcgtt
      361 gactttcttg cgtttacggc gacgtggagt atcttcatcc tcttcgtcca ttccatggtt
      421 tacttcttca tcgatcgaat cgatagcata ccatgaacgc aagccccaac gattatcccc
      481 taatgagata aagctgccgt caatattcaa gtcagtataa aattgtgcga gtgaatcacg
      541 aatttcactg tctgatttcc caagatagtt ttggatctgg ttagctaaat cagaaaaatc
      601 catgacatcg ccacgttgtt ctaagatcgc gtgtgctact tcgatcattg ataattcgct
      661 tttgtttgca ccttcaaata cactaatttc caatcaggac acgtcctttc acagtctact
      721 ccttatcata caaaatcaga cacaaaaaat caaacttttc ttttatattt acgctgtaaa
      781 atccaaggtc aaataatatt cgccgatttt ttccggtccc atgaacagat cataatcgat
      841 tcgaaccttc cctcctgtgg gacggtcttt caaactaaca tgcagttctt tcgtaaaagt
      901 agcaatctgg atcataccgt atggtgttcg ataggatgtt tctaaccgtt ctttatagcc
      961 aaatttcaaa cgcatccgca tttctcctgc acggatcagc tgtacatgac catctggaac
     1021 taatttgatc gttacaggaa tctcatcgcc cgtctcctcc tgaatctctt tataacgtat
     1081 gtacagcgta tcgccaattg tcaccatttg ccctttcaaa tcaaaataaa aatcttgcac
     1141 ttccccttgc tgttttactt ttgtacgcaa tttgattgaa atcggtgttc cttgtgataa
     1201 ttccattatt cattctcctt tacttctcat agctacattg ttcctagttt cttttgtttt
     1261 gtcaaccaac ttgtccattt ttacagaaaa ccttttttcc aaaacaactc tttgatttta
     1321 gagtataata ttgtagaaaa tatgaattta tacaaaggag ccggttgaat gaaccctgcg
     1381 attcgactag atcagcacat ttatcaaaaa aacgactttt ccccttttgc attaacagat
     1441 atattaacgg aatattctaa agaacaccag caaatctttt tacatttctg gcaatatgac
     1501 tgcacagtca tcttaggtat gaaggatgtt cgaactcctt ttctaagtga cgggatccaa
     1561 acactgaaag atgcaggtta cgctccagtg atcagaaatt caggtggatt agggatcgta
     1621 tcagatgaag gaattttgaa tatttctttg attttccccc aaagtgacac aaaaaaaata
     1681 accatagatg acgcttacga gcaaatgctc ctattgacta aatacgcttt tccaaaggca
     1741 ccgatacaag cattcgaaat cacccattct tattgtcctg gaacttatga tttaagcatc
     1801 catggcaaaa aatttgccgg tatcgctcag cgtcggatca aaaatgggat ttctgttatg
     1861 atgtacttaa gtgtcaacgg taatcaggaa aaacgcgggc aagtcatgaa gcgattttat
     1921 caatctgctt taaaagaaga ctttggtaca aatggctatc ctgcagtgga tcctgctaca
     1981 atggcaacac ttgaagaact gcttgaccaa cccctttttg taaaagaagt caaagaagca
     2041 tttgcaaaag cttttgaaaa gctgtattct gcaagtgatt tacaggattc taccgactgg
     2101 ctaaaaaaag aaatccagct ggagcaatgg aacaaccaaa tccagagaat ggaacaacgt
     2161 aataacataa aggagctaca ttatgacaac cccttataaa tatcaaatgc tgcctatgga
     2221 aaaagttttt cgtgatccag tccataacta tatccatgtc cagcatcaag taatcttaga
     2281 tttgatcaat tcgaaagaag tccaacgtct acgacgcatt aaacaattag gcacttcttc
     2341 tttcactttt cacggagcag aacatagccg ttttactcat tcattaggcg tttatgagat
     2401 ttcaagacga atctgcgata tcttttctcg aaatttttca aaagaaaaaa taggcaacgg
     2461 aggctgggat gatcaggaac ggctggtgac tttatgtgcg gcactgttgc atgatgtcgg
     2521 tcatggtcct tactctcata catttgaaca tatcttccat acgaatcatg aagccatcac
     2581 tgtagagatc atcacttctc cagaaacaga agtgtatcag attcttaacc gagtagaaga
     2641 aggttttcct gaaaaggtag ctagtgtgat ccaaaaaact taccccaacc cacaagttgt
     2701 ccaaatgatt tctagtcaga tcgatgcaga tcgaatggac tatttgcttc gggatgctta
     2761 tttcactgga acagaatatg gaacatttga tcttacccga atattacgtg tgattcgtcc
     2821 ttatgaaggc gggatcgctt tttccatgag tggcatgcat gcagtggaag attatatcgt
     2881 tagccgatat caaatgtatg tgcaagtgta cttccatcct gtttcccgtg gtatggaagt
     2941 aattttggag catctgcttc atcgcgcaca cgagttgtat caagaagatg ccagcagttt
     3001 tgaactccat tctcaattac ttctgccatt tcttgacgga agctttactt tagaagaata
     3061 cctcaaactt gatgatgggg tcttgggcac ttactttact caatggtctg aatctgctga
     3121 tcctatttta aatgatttag ctaaacgttt tttgaatcgt aaaccactga aatctgctac
     3181 tttttctggc aaccgggatt caaaattagt ccaagaactc actttgttag tagaaaaggt
     3241 cggatataat cctgtttatt acactgctgt gaattctagc tacgatctac cttatgattt
     3301 ttatcgccca gaacaaggca gacatcgcac acaaatcgag attttgcgaa atgacggaac
     3361 tttgatagaa ttatcacagg ttagtcagct tgttgctgca ttagcgggac aagaacaagg
     3421 agatgagcga tttttctttc caaaagaaat ggttgatccg tccttgcgtg atcattatga
     3481 tttatttgac gaaacttatc aagaatttgc cagccatatc cgtaatggcg cattgattga
     3541 aataaattaa aatatattgg aggaaattat gtctatcaaa ctagtcgcta tcgacatcga
     3601 tggtacttta ttaaatgaga aaagagaagt aacaaaagaa gtaaaagaag cgattgccgc
     3661 tgctgttgca aaaggtgttt cgatcgtttt atgcactggc cgtcccttac caggtgtcca
     3721 ggaacagtta aacgagttga acctttttca agataatgat tacgttatta cttataacgg
     3781 ggcactcgta caacaaacga aatcaggtaa aatcatcgcc cgtcacggac tgacacatga
     3841 agatttccta gagatcgaag tgatggcacg ccgtgtcggc agtcatctcc attcgatcga
     3901 tgacaaagcg atctacacgc ctaatcgaga tatcagcgct tatacgatcc acgaagcaag
     3961 tttagtcaag atgccgctaa aatatcgaac acctgaagaa atgacaccag acatgaacat
     4021 cgtcaaaatg atgatgatcg acgaaccaga aattttagat gcagcaattg cgcgtttacc
     4081 gcaaactttc cgtgataaat acacaacggt caaaagtgcg ccctattact tcgaagtact
     4141 aaataaagaa gcaagtaaag gtgcggcagt tgcgaatctc gcacagcacc ttggtattga
     4201 tcaagatgaa atcatggcta tcggagataa cgagaacgac ttatccatga ttgaatatgc
     4261 tggtctagga gtagcaatgg gaaatgccgt ttctcttgtc aaagaagcag caaatgtcat
     4321 cacttcatca aacgacgagc acggtgtagc tgaagcaatc aa
//
LOCUS       DASHFF010000124         4350 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.126, whole genome shotgun sequence.
ACCESSION   DASHFF010000124 DASHFF010000000
VERSION     DASHFF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.126"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            55..1428
                     /gene="tet(L)"
                     /locus_tag="U2I75_002764"
     CDS             55..1428
                     /gene="tet(L)"
                     /locus_tag="U2I75_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HEN1697813.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            1992..3254
                     /locus_tag="U2I75_002765"
     CDS             1992..3254
                     /locus_tag="U2I75_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEN1697814.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3499..>4350
                     /locus_tag="U2I75_002766"
     CDS             3499..>4350
                     /locus_tag="U2I75_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEN1697815.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1516 a          589 c          893 g         1352 t
ORIGIN      
        1 gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggaatt aattatgaag
       61 tgtaatgaat gtaacagggt taaatacaac caaattttaa tttggctttg cgttttatct
      121 ttttttagcg tattaaatga aatggttttg aacgtctcat tacctgatat tgcaaatgat
      181 tttaataaac cacctgcgag tacaaactgg gtgaacacag cctttatgtt aaccttttcc
      241 attggaacag ctgtatatgg aaagctatct gatcaattag gcatcaaaag gttactccta
      301 tttggaatta taataaattg tttcgggtcg gtaattgggt ttgttggcca ttctttcttt
      361 tccttactta ttatggctcg ttttattcaa ggggctggtg cagctgcatt tccagcactc
      421 gtaatggttg tagttgcgcg ctatattcca aaggaaaata ggggtaaagc atttggtctt
      481 attggatcga tagtagccat gggagaagga gtcggtccag cgattggtgg aatgatagcc
      541 cattatattc attggtccta tcttctactc attcctatga taacaattat cactgttccg
      601 tttcttatga aattattaaa gaaagaagta aggataaaag gtcattttga tatcaaagga
      661 attatactaa tgtctgtagg cattgtattt tttatgttgt ttacaacatc atatagcatt
      721 tcttttctta tcgttagcgt gctgtcattc ctgatatttg taaaacatat caagaaagta
      781 acagatcctt ttgttgatcc cggattaggg aaaaatatac cttttatgat tggagttctt
      841 tgtgggggaa ttatatttgg aacagtagca gggtttgtct ctatggttcc ttatatgatg
      901 aaagatgttc accagctaag tactgccgaa atcggaagtg taattatttt ccctggaaca
      961 atgagtgtca ttattttcgg ctacattggt gggatacttg ttgatagaag aggtccttta
     1021 tacgtgttaa acatcggagt tacatttctt tctgttagct ttttaactgc ttcctttctt
     1081 ttagaaacaa catcatggtt catgacaatt ataatcgtat ttgttttagg tgggctttcg
     1141 ttcaccaaaa cagttatatc aacaattgtt tcaagtagct tgaaacagca ggaagctggt
     1201 gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg tattgcaatt
     1261 gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat ggaagttgat
     1321 cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat tgtcattagt
     1381 tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat cgttaaggga
     1441 tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt caaatctttt
     1501 tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc ttgtatcttt
     1561 tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa aaattttatt
     1621 cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa ttggcaaggg
     1681 tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc ccttgctgat
     1741 ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg caggtttttt
     1801 tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt ttggtcggca
     1861 ctgccgacag cctcgcagag cacacacttt atgaatataa agtataatgt gttatacttt
     1921 acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac ctaaaaagga
     1981 gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca gctggactaa
     2041 aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat gatattgacc
     2101 atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat tacaacgaac
     2161 gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg aaagatgctg
     2221 ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa ctggatccag
     2281 gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga tatggcaagc
     2341 aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg catttaggtg
     2401 ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt caagaactgt
     2461 tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag ttgaagcgtg
     2521 gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa caaactttgg
     2581 aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa tggactgctt
     2641 atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg aaaagtgttg
     2701 aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg aaaacagaaa
     2761 agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta gtgactgctg
     2821 cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact gatatggcga
     2881 gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat agtggtcttg
     2941 tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa aacaagtctt
     3001 taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa agcactaagg
     3061 aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt aaggggtttg
     3121 tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta gaacctaaaa
     3181 agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt cgagatcagg
     3241 gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga tggttttgaa
     3301 cttgttcttt cttatcttga tatatataga aataacgtca tttttatttt agttgctgaa
     3361 aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct taaaattggt
     3421 tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac taaatagatg
     3481 ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga aaaatcaagg
     3541 gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa gaaaatcgct
     3601 aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga aagagtaaaa
     3661 gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag aaagttgtat
     3721 cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag agcaatgaaa
     3781 catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc aacagttcgt
     3841 tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt aaataagagt
     3901 ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat taataaaaat
     3961 cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa ttcttataat
     4021 cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac agaaaactac
     4081 gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga ctatgatcca
     4141 aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat acaatcggca
     4201 attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga tgatgaagaa
     4261 aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag gttaatctcc
     4321 tatggtggtt tgttaaaaga aatacataaa
//
LOCUS       DASHFF010000125         4261 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.127, whole genome shotgun sequence.
ACCESSION   DASHFF010000125 DASHFF010000000
VERSION     DASHFF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.127"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(65..1285)
                     /locus_tag="U2I75_002767"
     CDS             complement(65..1285)
                     /locus_tag="U2I75_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEN1697816.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="U2I75_002768"
     CDS             complement(1305..1916)
                     /locus_tag="U2I75_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1697817.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="U2I75_002769"
     CDS             complement(1959..2891)
                     /locus_tag="U2I75_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN1697818.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="U2I75_002770"
     CDS             complement(3312..4172)
                     /locus_tag="U2I75_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1697819.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1211 a          860 c          700 g         1490 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 a
//
LOCUS       DASHFF010000126         4093 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.128, whole genome shotgun sequence.
ACCESSION   DASHFF010000126 DASHFF010000000
VERSION     DASHFF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.128"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..153)
                     /locus_tag="U2I75_002771"
     CDS             complement(<1..153)
                     /locus_tag="U2I75_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1697820.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="U2I75_002772"
     CDS             complement(475..696)
                     /locus_tag="U2I75_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697821.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="U2I75_002773"
     CDS             complement(794..1039)
                     /locus_tag="U2I75_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697822.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="U2I75_002774"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="U2I75_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..3264)
                     /locus_tag="U2I75_002775"
     CDS             complement(2263..3264)
                     /locus_tag="U2I75_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN1697823.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3423..3689)
                     /locus_tag="U2I75_002776"
     CDS             complement(3423..3689)
                     /locus_tag="U2I75_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1697824.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3679..4035)
                     /locus_tag="U2I75_002777"
     CDS             complement(3679..4035)
                     /locus_tag="U2I75_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1697825.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1295 a          744 c          542 g         1512 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
     2641 atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt ttttgttaat
     2701 tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt ctaaattatt
     2761 ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga tattgtcttt
     2821 gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct taacttctat
     2881 atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc ctacctcgcc
     2941 tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat aattatcaac
     3001 tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt ctacaatttg
     3061 tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg cattcgtttc
     3121 ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat tccatctata
     3181 tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg ctataaatac
     3241 ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc tttaaagtaa
     3301 tttttgtata aaatttattg acttttacac tataggacaa cttttatcta tttagtacaa
     3361 aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt ttcaactcgc
     3421 ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc atcaagttta
     3481 tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt tgtatgtgct
     3541 ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata aagagcaacc
     3601 gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt gtctttttgc
     3661 ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa gttcttttgc
     3721 atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat gggtaatgac
     3781 ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc ctaccttact
     3841 tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt gagctactag
     3901 catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat ctttttttgg
     3961 taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa atgtttcaaa
     4021 agatattttt tccataccca attatatcat atattgattt tatattatag tatataacat
     4081 aaaaattcga att
//
LOCUS       DASHFF010000127         4082 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.129, whole genome shotgun sequence.
ACCESSION   DASHFF010000127 DASHFF010000000
VERSION     DASHFF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.129"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            101..1450
                     /locus_tag="U2I75_002778"
     CDS             101..1450
                     /locus_tag="U2I75_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1697826.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1578..2168)
                     /locus_tag="U2I75_002779"
     CDS             complement(1578..2168)
                     /locus_tag="U2I75_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1697827.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2315..3667
                     /gene="brnQ"
                     /locus_tag="U2I75_002780"
     CDS             2315..3667
                     /gene="brnQ"
                     /locus_tag="U2I75_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1697828.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1179 a          745 c          791 g         1367 t
ORIGIN      
        1 aaaggtaaat ttctgaaaat tttggaatct tagttgtgtt tttcagaaaa ctctcttaat
       61 atggaagtaa ttaaactttt ttagttagga tgatttaaaa atgaatgaag agcaagaact
      121 gcagcgtgga ctgaaaaata gacatgtcca actgatttct attggcggag cgattggaac
      181 ggggttgttt ttaggttccg gaaagtctat ccagctagct ggcccttcga tattgctcgc
      241 ctatcttatt actggatgca tctgtttttt cattatgcgt gcgttaggcg aacttctgtt
      301 atcaaacacc aataaccatt cttttctgga ttttgttgca gaatatcttg gcaaaaaagc
      361 ggctttcatc actggctgga cgtattggtt ctgctgggta tcgattgcaa tggccgattt
      421 aacagccatc ggaatgtatg tacgctactg ggcgcctggt gttcctcagt ggcttcctga
      481 actgattgca ttaggtcttc ttctttgctt gaatatggtg gctgtctcat tgtttggtga
      541 gttagagttt tggtttgcat tgatcaaagt tgtagcgatc attgccttta tcctcgtagg
      601 agcttatatg atcctcaccc actatacgac agatattggc acagccagta tcacgaatct
      661 atggtctcat ggcggattct ttccaactgg tgcaaaagga tttatcttag ctttccaaat
      721 ggtaaccttt gcttttgctg ggatagaatt agttggatta gtcgctggag aaacagaaaa
      781 tcctgaaaaa gtgttgccgg aagctatcaa taatattcct atcaggatca tcttatttta
      841 cttaggatct ctttttgtca ttatggcaat ctatccatgg aacagcttga atccagataa
      901 cagtccattt gtagaagtat tttcggaaat tgggatcacg attgctgctt ccttgatcaa
      961 tttagtcgta ttaagtgcag cagcttctgc ctgcaacagt gcaatatata gtacaggacg
     1021 tatgcttcgt tctttagcgc aagaaggcag tgcccctaaa aaattcaaaa agttaacaac
     1081 acatcatgtg ccagggaatg ctttgacatt ttctactatc gtcattttca tttctgttat
     1141 cttgaattac gtgatgccta gtgaagtatt cacactagta agcagtattg ccactacttg
     1201 tttcttgttt atctggagta tgcttgtcta cacgcacatg aaatatcgta aaagtatcct
     1261 cggcaaaaaa gctcattcat ttaaaatgcc gttgtaccca tttagtaatt atctggtatt
     1321 tctttacatg gcctttgtgt gtgtcgtctt gttcctagga aaagatacga gaatcgcttt
     1381 attactaaca cctgtgtggt tcttgcttct attactaatc tatcatatga aatatgaacg
     1441 taagaagtaa gaaagtagtt gtgacagaag tggtcagctt cgagaaataa gaaggaattt
     1501 acgaaaattg cttcttaaat ttttgtgaaa tttcggctta tttcttggag ataaaagctt
     1561 ctgtcccagc ctcttttcta tttccccgga aacaaccgct gataataggc atctgctttt
     1621 ttataggaaa aaatccaaat accagataca aaaaaacgtc cgaataaccc gatcaacggt
     1681 acaaaaaaga caccggcaat caagcataag ttaatcatca tggagataag acttcgacgg
     1741 tcctttgaca aaactctcac gacatgggaa tctggtttat ttgctttcat gatttgcctc
     1801 atcaagatac caaaagaaaa actccacatc gtgtatacta agacataagc catctcaggc
     1861 caaaaagctt cagggttacg cccgacccaa ctagtaacag aaggacataa agtcagccaa
     1921 aagagcaaaa agatattcga ccagaccact cggtaattaa ttcgttctgc catctgaaat
     1981 atacgatgat ggttcaccca gatcaccgca acaaatataa agctgatggt ataagctaat
     2041 aatgtatttc ggatgaccca taaagaatga aagctagatg tattagggat cttgatctct
     2101 aatacaataa ctctcaaaac aatggctacc actccatcag taaatgcttc taatctcgac
     2161 tttgtcacga tgattcctcc tcagatgatt cagcgctttt attttatcac ttacgttatt
     2221 aaataaccat cgttttatat agggaatttt ctgaaaattg ttgcatattt ttcgaaattt
     2281 tgttactttt aaaaaaaccg taaaggagtt atgtatgaca aaaaaattat catttaaaga
     2341 ctatcttttt ataggctcca tgcttttcgg tctatttttt ggtgctggaa atcttatctt
     2401 tcccgttcat ttgggtcaag aagcaggcgc tgctaccttt tgggcgaatc taggtttctt
     2461 ggttaccggt atcggtcttc ccttcttagg ggtgattgcc attggtgtat cgaaaagctc
     2521 aggtgtatat gaattagcaa aacggataaa tcaaacatat gcgatggttt ttaccatctt
     2581 gctttactta gttatcggac cgttttttgc tttacctcgc cttgcaacca catctttcga
     2641 gattggttta gcaccattta tcgatcctag cagacagacg ctatatctag caattttcag
     2701 tattgtattt tttgtgctgg cttggtggtt ttcaagaaaa ccgacaaaga tattagatta
     2761 tgttggaaag ttcctgaatc ctgcattttt ggttttatta ggtgctttgc ttctccttgc
     2821 ttttttgaat ccactaggtt ctgtaaatca agcgcctatt cagccaaatt atcaagagca
     2881 tgcttttttc acagggttta ctcaaggata taacacatta gatgcgcttg ctgctttagc
     2941 atttgggatc atcattgtca caacaatcca aaacatgggt gtaacaaaac ctgctgaaat
     3001 cgctaaagac acgatcaaat ctggtgcgat cagcattata ttgatgggaa tcatttatac
     3061 tttgctcgct tatttaggtg ctatgagctt aggtagcttt gctctaagtg aaaatggcgg
     3121 gatcacgctg gctcagattg ctgatcatta tttaggtact tatggcagta tcttattagc
     3181 atttatcgtt atattagctt gcttgaagac cggtattggt ttgattacag cttttgcaga
     3241 aacattcaca gatttgtttc cgcaaagaaa ttacgcattc tttgttgcac ttgctagtat
     3301 ccttccttgt ttagcggcaa acgtaggctt gacgaatatc attcaatttt cgttgcctgt
     3361 tttgatgttc atctatccat tagcaatgac actgatttta ctagtgttag ttggtccttt
     3421 gtttaagcag cgtacggctg tttatcgaat gactacttat tttacattga ttgcttcgat
     3481 ttttgatggg ttgaacgctt gcccagaaac tatcaaacaa acacctattg ttcaaaatct
     3541 tttatatgtt gcggaaagct acttgccgtt tttcaaattg gggatggggt ggatcgtacc
     3601 agcagttatc ggttttgtta tcggactgat ttggagtttc gctaagaaag aagaagtagc
     3661 agattgataa aagaggttgt gaaagagacg ttcagccttg agcaataaaa agtaattttg
     3721 aaaaatggct tctcacattt ttgcataaat ttgacttaat accgagaaac tgacttttga
     3781 acaccgttta ttgagaggtc gtgaaaaagc cgcttagttt caagtattaa gaaatatttt
     3841 gtgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt
     3901 gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa
     3961 gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac
     4021 aacatttctt ttataatata gattttagac atatatatga tataaaaata gttttcgcaa
     4081 aa
//
LOCUS       DASHFF010000128         4034 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.130, whole genome shotgun sequence.
ACCESSION   DASHFF010000128 DASHFF010000000
VERSION     DASHFF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.130"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            73..234
                     /locus_tag="U2I75_002781"
     CDS             73..234
                     /locus_tag="U2I75_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEN1697829.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="U2I75_002782"
     CDS             253..1530
                     /locus_tag="U2I75_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1697830.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="U2I75_002783"
     CDS             1540..2196
                     /locus_tag="U2I75_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1697831.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="U2I75_002784"
     CDS             2196..3572
                     /locus_tag="U2I75_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1697832.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="U2I75_002785"
     CDS             3702..>4034
                     /locus_tag="U2I75_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1697833.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          440 c          704 g         1278 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DASHFF010000129         3986 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.131, whole genome shotgun sequence.
ACCESSION   DASHFF010000129 DASHFF010000000
VERSION     DASHFF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.131"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..391
                     /locus_tag="U2I75_002786"
     CDS             <1..391
                     /locus_tag="U2I75_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HEN1697834.1"
                     /translation="RKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSYHVRNIDLMQS
                     TGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCATVDEDLEYQIF
                     NTELFEEYTYEVTGNIYENSELLEEQR"
     gene            388..945
                     /locus_tag="U2I75_002787"
     CDS             388..945
                     /locus_tag="U2I75_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN1697835.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            942..1238
                     /locus_tag="U2I75_002788"
     CDS             942..1238
                     /locus_tag="U2I75_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697836.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1315..1728
                     /locus_tag="U2I75_002789"
     CDS             1315..1728
                     /locus_tag="U2I75_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN1697837.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            2037..2189
                     /locus_tag="U2I75_002790"
     CDS             2037..2189
                     /locus_tag="U2I75_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697838.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(2186..2461)
                     /locus_tag="U2I75_002791"
     CDS             complement(2186..2461)
                     /locus_tag="U2I75_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697839.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            2915..3121
                     /locus_tag="U2I75_002792"
     CDS             2915..3121
                     /locus_tag="U2I75_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697840.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            3317..3484
                     /locus_tag="U2I75_002793"
     CDS             3317..3484
                     /locus_tag="U2I75_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN1697841.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            3510..3854
                     /locus_tag="U2I75_002794"
     CDS             3510..3854
                     /locus_tag="U2I75_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1697842.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         1379 a          613 c          855 g         1139 t
ORIGIN      
        1 acgaaagaac gtaatgagag atgtagccgt cttgcatttt actaaaaacg gcaaaacaaa
       61 ctttattgaa tattggataa atcctaccga attgaaatca tatcacgtgc gaaacatcga
      121 cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg gatatatttg aaggggatat
      181 agtattagtc agcgtgcgaa atggcttcga ttacttagat aataaagtct gtattgtcaa
      241 aaattcaata gattattccg gattagtttg tgccactgtt gatgaagact tagagtatca
      301 aatttttaac acagagctgt ttgaagaata cacgtacgaa gtcaccggaa atatatacga
      361 gaatagcgag ttattggagg aacagcgatg aataaacagg aagcaatcaa aaagttagaa
      421 agtattaaag cgataggaaa tgatgcaata gctgcttgct ataacgagag tataaattca
      481 ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac cagttattcc acaactcgtg
      541 gccggttggc ttgagaaatc tacggaccct tttacaaaag ctgaaaaaat agcgtattta
      601 atcaaatcta aagatggtga ttcatattat ttctgtgatt ggtttgtacg agatggcata
      661 gtgacgcaag agcaaggaga agaattactc gcttgggcaa caagacaatc atatgaaaca
      721 ctattgagcc tatacaacgg ctacgaggtt gagaaagagc cgttatgggc aataaagaat
      781 gccgatggaa actatcttac taaatgtgct ttatggggaa aagatggagt aaattatagt
      841 tttgaatgca atccatctca tcgattgctt ttcactgata aagcaacagc ggatgctgca
      901 gcattgttgg tgaatggaac agtggaagag gtggtagaaa gatgaaacta aaagacggat
      961 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta
     1021 aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt
     1081 tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta
     1141 ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag
     1201 cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact
     1261 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
     1321 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
     1381 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattata
     1441 acggatatgc cgaaggcacc gaagcatggc aataaggcag aagacgcgat tattcagatg
     1501 atggatatag aaacggagag agatgcgatt ctagcggctt tgatggctct tagtctgatt
     1561 agtcgtcaga tactctacta cagcttctgt gtgccagata gcttctcaaa ctacagaatt
     1621 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaagtcgga agctctaata
     1681 gagtttgcag aagcatataa acacggaaaa ataattgctt ataaataatt tggcggtttt
     1741 ttggcggaat gatggcggtt tttagacatt taccagtgtt attatggtag tgtcgaaaga
     1801 ttaggaaaca ggatcgacaa aataaacgaa gggaggcaat ctccctcatc gttttaaact
     1861 tcttcttaga tagacagcaa ctaattatga agaaaaggat gtgaattcaa ctcctcctaa
     1921 attcttcatc ggttgctgtt tattttgatt agacagcagc actcacaaaa ctaaacatgt
     1981 tcttgtcgtc tttcaactgc tgctgtctat taatttatgt attggaggga agaaaaatga
     2041 aattatcagt agaaggcact ccagaagaaa tacaagaatt gctccaagct attggtagta
     2101 gcaaggagca aaaggtaagt gtttctaatt cagatattga cggtatttgc aattgtttaa
     2161 acagagaaaa aatatctaat ccttactaag agaaaatatg ttcgttaatg aatttccaat
     2221 tttcctctgt agctctacct tgataatttt tttgcagttc acagatgaat aaggaatcgt
     2281 tattatcaag tactgtattt aatttttcac acatttgatt tggattcaaa ttagttttaa
     2341 ctaaccaaaa agatttttgt agctttatat atgccccaaa ttcttttatt atttcaaata
     2401 ctttatcata cttttgccct gggttattaa gatcataact aatcatgtag ggtttataca
     2461 tgttgtcttg cacatccttg ttttatattt cagcggacca ctcgctgata aataaaatta
     2521 tacgcttagt atttattttc acaatattaa tttatcgtaa taacttttga gtaaatagaa
     2581 caaaaaacct gcactagttt ccgctagtac aggcagtgac tatatcggtt gataatctag
     2641 catatattaa aaataattgc aagaaaagaa atttatagtg gtttatacca aattatcaaa
     2701 gcaatgttat tttgttgctg tctattgttt tttaattatt cacacgataa ctaaaggtgg
     2761 gtgaagagaa atgattccat taataatttc aatttttgcg ctctgtctta atgtctatat
     2821 gattggattt aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg
     2881 tcactgtggc ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca
     2941 tgcaaggttc gattccttgc cagcgacatt aaatgcctat gacggttacg actaccgaaa
     3001 aaagatcgtt aagaagctat acggtgctac gtacggcaat gtagtaagtg tgctatctgt
     3061 acaccaccaa gcttcggtca ctgtggcgga agtagaagac gcagcggtaa atggcgagta
     3121 gcctcgtgag agcctggtaa gttctcgtga gtggtgcaat cccactccag cgactttaag
     3181 caaccgaggc atcggcggtt taaaaatata ggggtgcgca atttcgtacg cgttttgtgc
     3241 atcgtgcaag ttactattac atattagatc actctttgag tggtcttttt atttttgcac
     3301 aaaggaggaa acaacaatgt atagaccaca atacttagaa cagaagtatg aagtaatcac
     3361 tgttcataaa ggtaatggcg aaaaagtata tgagtataga agaccaataa agagcgatac
     3421 atataaacga aaggaaaaca atgaagttat tccattgtat ggcaaaagaa tagctaagca
     3481 ttaaataaga ttgcgaaagg agacggaaca tgaccgagga attctataga tggctattac
     3541 agttgacaag agaagatcgt ttggttaagt tctatcagtc tcctaaatgg cgcaggctta
     3601 gagagaaagc gatgaaacga gatcactatg aatgccaaga gtgtagaaga ctaggtaagt
     3661 atcatagagt agagaacgtt catcatataa aggaagtcaa ggatagacct gacttagctt
     3721 tagatttaga taatcttatt tgtttatgtg ttgaacatca taatgaagtt catggcagat
     3781 atcttacagc attagataaa caagagaaga agatagaaag ctttgctaac ttcgatgcaa
     3841 gtgaaaggtg gtaagtgcat gatcatcaat gataatggca gagagtatga tacagaaaag
     3901 attgaagagt attcatctta tactcaggga ttaattaaac gtttgatata cgttcgctat
     3961 gtaggtatta gggatctgtt atcaga
//
LOCUS       DASHFF010000130         3904 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.132, whole genome shotgun sequence.
ACCESSION   DASHFF010000130 DASHFF010000000
VERSION     DASHFF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.132"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..204)
                     /locus_tag="U2I75_002795"
     CDS             complement(<1..204)
                     /locus_tag="U2I75_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1697843.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMK"
     gene            complement(201..425)
                     /locus_tag="U2I75_002796"
     CDS             complement(201..425)
                     /locus_tag="U2I75_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN1697844.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(422..715)
                     /locus_tag="U2I75_002797"
     CDS             complement(422..715)
                     /locus_tag="U2I75_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697845.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            complement(753..890)
                     /locus_tag="U2I75_002798"
     CDS             complement(753..890)
                     /locus_tag="U2I75_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN1697846.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            complement(892..1338)
                     /locus_tag="U2I75_002799"
     CDS             complement(892..1338)
                     /locus_tag="U2I75_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697847.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(1335..1484)
                     /locus_tag="U2I75_002800"
     CDS             complement(1335..1484)
                     /locus_tag="U2I75_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697848.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1501..3621)
                     /locus_tag="U2I75_002801"
     CDS             complement(1501..3621)
                     /locus_tag="U2I75_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697849.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVIFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGA
                     DYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTTPTFSPQAYHP
                     WIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLGENLITNAKFP
                     IITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQLQYQATLLPV
                     EGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYGTVQIDWLKIEKGDTRTPNIEQ
                     YKYRGIGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQL
                     TASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYR
                     EGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGA
                     DGAIKWRRNSSYGSDYTFAITYTI"
     gene            complement(3645..>3904)
                     /locus_tag="U2I75_002802"
     CDS             complement(3645..>3904)
                     /locus_tag="U2I75_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697850.1"
                     /translation="GKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFII
                     ETSKPNVLVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
BASE COUNT         1068 a          812 c          641 g         1383 t
ORIGIN      
        1 tttcatgttg gcggctacgt ttcttgctgg tgcatctatt ccagtttcgt gcaaaataat
       61 tttgttagga attgctaatc tggagtctcc ttgattcggc gctaaattat actcgtcatt
      121 aatagtataa gcaaacgtat taatgggtaa taaaaaaaga gccgttaaca ggctcatcgc
      181 agtaatagta attttctttt tcatttgttt cctccttctt cgctttcagc cgagaacatt
      241 ttgtaggttc gatttgatac acccaacaca ctccctaaaa acgcgccaaa accagtaatg
      301 atgacaacac agatatctgt gtactgccaa ttgagcgctt taccaactaa ccccacgaaa
      361 gtagctagtg cgggaataat taccagtgcg aaccatttta gtacttcgaa cgttttatta
      421 ttcattttct tctctcccta aataaagttt taatttgttg cgtgtgttct accaattttt
      481 ctgcatgtgt atctaatctt tcatcgtgtt tctttagttc ttcatgaatc atcaatcgat
      541 ctgatttgct cgattctaaa tctttagtca gcaaatctaa attgtgactt acttttgaaa
      601 gagtctcagt aatcttcgag aaagatgcag taattggttt tattactaat aaaatcaaag
      661 aaacgatagc ggttattgat cctgctatcg ctccccattc ccctaaatta atcatgtgac
      721 aactccttga atcaaaataa aaagcacatc aattaagatg cgctctcttc tttgctaatg
      781 attttatctg ctttttcttc agtaatgcac aacggaacga aaaccattac ttgttcgtta
      841 gtgaaacagc cccaatcata catcattttc acatcgctaa aactaaacat actactcacc
      901 tccctttgaa gctggattta gttgctcttt aatttctgaa atgtctttgc tattttgtaa
      961 cgaagcaagc atcatttttg aattgatttg tgctaaacta tccgcttttt ctttcaatgc
     1021 agtattttcc tgtttaattg ctacatcgct tagcatgagt ttggcattga tctgttttaa
     1081 atcgccgttc tcattttcta acgactcata cattgctttg agattgttta aatcgttgtg
     1141 atctagtgcg ttcgctaaaa taatccattg attcaattta ggatcaaaca tttgatcagc
     1201 gattgttaac ggttcgccat cagcacgaat tccttcaagc ggtggctgat ctgtgtaagg
     1261 aacggataca agcatgtcgt ccaatacttt tcctgcgtac tctccgccag tacgtccata
     1321 tttccaaatg tttttcattt atttccctcc cagtaattga atttcggttt ccaatttgga
     1381 atcggtggtt cgacttctgt gcattcttcc ggtaaatgtt cttcatcatt cacaatgatt
     1441 tgctcgaatc cgtaaggttc aattggtcta tatgctgcct tcatatcgat tcacgctttt
     1501 ctaaatcgtg taagtaattg caaaggtata atccgatcca taacttgagt ttcttcgcca
     1561 tttaatggct ccatctgcac caatagataa ctgagcactg ttcaaagtag aacggtctat
     1621 cgagccaacc agttgctcaa aactaattgg tggccgatag ccttctggaa ttgttagtat
     1681 cgttgaatca tttccaccac tgctttttcc gtttaaagcc acaaaatata tagaaactgt
     1741 ttttccttca cgataaagct ttgctgatcc ggtattcccg tttgtaactg ttaatgtggt
     1801 agtagctgta tcattaatgc gttcatcgat cttattgtct aattcatcta tagcagtcgc
     1861 attagcattc gcttttgttt gagcatcctt agctgtggtg tctacttcat taattgaagc
     1921 agtcaactgc gaattaatct ccgatacttt cccatcggta taattgtttg ctttaccagt
     1981 aatttcagaa attttaatat ctgtggccaa attatcttcg acatattctg gtgctagatc
     2041 ccaaacataa tcttttggat tgtttgaatc acgcatgccg attcctcggt atttatattg
     2101 ctcaatattc ggagttcggg tgtcgccttt ttcgatcttg agccagtcga tttgaacagt
     2161 accatatgtt gcagaattag gtttttgcca taacgctact ctaagtaaat ttgtcgtttc
     2221 actagtattt ctggctgtaa aagtggcact ccacacatct gttaatcctt ccacaggaag
     2281 taaagttgcc tgatactgca actgctggta atttgcttct ccgtagtata agtggaattc
     2341 tttagtagct ggcttagtcc cttttaaaga taccgtataa gtttcaccta ttattaattc
     2401 ttctgaaata tcaaaaccag aaataggatt gcttcgtgtt ataattggga acttggcatt
     2461 cgttatcaaa ttctcaccca acggtgtttt acctagccag taagagtcat caagtaagtt
     2521 aggctgatat ggtgtagctg tagagccttc ttcgattttg atgtcatagc tcatttctat
     2581 ttccccaagt accccatctt gagccctaaa ttgtatccaa ggatggtatg cttgtgggct
     2641 aaatgttgga gtagttccag taatactaaa ctctttccat tcctgagtta tcgaattact
     2701 gttaataggt aagtctaaaa caatcttgcc agttccactg tcaacatacc tatatctaat
     2761 cagtataagt tttccgtcaa ctggactata gtccgctcca gctctaagct taacagtcat
     2821 tacaagcgtt ttattagagg gtaaccagtt agctatacct cttcgtttat aatataactc
     2881 tactccacct tccacttcag ctgatatctt attatattta aacgagtttt cacccttaga
     2941 aagtgttcca gctgaagtat tttgattttc aattaagtcg tatgatagct tacttaacaa
     3001 attcggattc ccgctataat catagccccc gaagtcgatg ctgttactgt acatgacttg
     3061 caagttacct aatttagaaa tttcttcttt cagagcatct aacttgtctt gtagcgtttt
     3121 agcttgacca gttaaatcag taatctgttg atttaagcta tccactctac ctttaatttc
     3181 agccataaaa gcatcaaaag tttcgttata ctttcgaatc aactcttcca attgcgaaac
     3241 gtattcatcg gcttggcctt gcgaaatgtc agacactcct agtgagaaaa aaatgatgtc
     3301 ttgcgttgtt aaaatttgat taccttttct atattctacg tagcagtgtt tataatatcc
     3361 tgcttcactc ataaatgtgc catcaagaga aaatatgact tcttcactag ttacgctagt
     3421 tgcaacacta tctacgtaac ggttagatgg cgttgttcct tttaaagtaa atgttccgcc
     3481 actcgtatcc atctgcaagc catttaaaaa tggtttaaca gtcactgtaa tccctttatc
     3541 accttgacga gccataatag ctttggtgta gtttaattct ttgctgaaat ctagagccaa
     3601 attatataaa ctgctagcca tttatatacc tccttgtctt cgttttaaaa acgtttttgg
     3661 tcaagcactg tgctatcata tgctgtatcc tcttttaatc taatatcttc ataccctaga
     3721 cggtgtgcca ctaaattcca tctaactaat acgtttggct tactagtttc aatgatgaaa
     3781 tggtcaatat cttcatgagt aacagcacac aaaactagtt ctgtaggtgt cacatgtgtc
     3841 atataccgac ttagatttac tgtttcagca aacatggggt caatatcaac acgaacttta
     3901 ccat
//
LOCUS       DASHFF010000131         3663 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.133, whole genome shotgun sequence.
ACCESSION   DASHFF010000131 DASHFF010000000
VERSION     DASHFF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.133"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..276
                     /locus_tag="U2I75_002803"
     CDS             <1..276
                     /locus_tag="U2I75_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEN1697851.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="U2I75_002804"
     CDS             419..640
                     /locus_tag="U2I75_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1697852.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="U2I75_002805"
     CDS             656..1498
                     /locus_tag="U2I75_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697853.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="U2I75_002806"
     CDS             1561..3363
                     /locus_tag="U2I75_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEN1697854.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="U2I75_002807"
     CDS             complement(3422..>3663)
                     /locus_tag="U2I75_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN1697855.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DASHFF010000132         3470 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.134, whole genome shotgun sequence.
ACCESSION   DASHFF010000132 DASHFF010000000
VERSION     DASHFF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.134"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            443..649
                     /locus_tag="U2I75_002808"
     CDS             443..649
                     /locus_tag="U2I75_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN1697856.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            649..900
                     /locus_tag="U2I75_002809"
     CDS             649..900
                     /locus_tag="U2I75_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697857.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            916..1353
                     /locus_tag="U2I75_002810"
     CDS             916..1353
                     /locus_tag="U2I75_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697858.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            1346..2041
                     /locus_tag="U2I75_002811"
     CDS             1346..2041
                     /locus_tag="U2I75_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697859.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            2422..2601
                     /locus_tag="U2I75_002812"
                     /pseudo
     CDS             2422..2601
                     /locus_tag="U2I75_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2663..2847
                     /locus_tag="U2I75_002813"
                     /pseudo
     CDS             2663..2847
                     /locus_tag="U2I75_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            2881..3312
                     /locus_tag="U2I75_002814"
     CDS             2881..3312
                     /locus_tag="U2I75_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN1697860.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         1239 a          571 c          714 g          946 t
ORIGIN      
        1 agaagagctg cagtcctaaa tgaggattgc agctcttttt gttttacata gaaacaagaa
       61 aaatgaaaag gcctttttag gaaggcgcaa gcgccttctc aattgaaaaa cctctctctc
      121 actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa cgttgcacgt tcactaagat
      181 aaggggtctt gcaaagcaag acaaaataga aaaaacttcc tctcaccaaa aggttcgttc
      241 ctgttcgcta tctctttatt gatttgccag accggcaaca agttgccttt tatcaccaaa
      301 aagcgacaag gacaagggta aatggcacgc tgcacgccct tgtccttgtc gcttttagtt
      361 gattcattct ggccaacaaa tcaataaaga aagaactaac gaaaggaagt aaaacctttc
      421 ctaagaggag gcagaatcaa tcatgcgaaa gaaatttgat tattggggtg ttcccttcag
      481 cgaattattc ccaaactatc atgcaccata tactgtcgaa tgcgattgtg gagaaagagc
      541 gaaatgtatc aaaagctatt gtttgtatca atgtccaaca tgcgggaaaa aatatacctt
      601 gtcttatggc gattatattt tgattgatga gaaaggaaag aaacgctgat gaaaacacaa
      661 cacaaattat tgactgtaaa agaagtcgtt caaattttgg tggacaaaga agtcatttct
      721 gaaaaagtag cagaagcttt acttattccg cggtactgga cgatagaaac ccctttagta
      781 gaggatgcac cgactatgct atttgaagac ctagtgaaga ttgcaggata tgaactgcat
      841 tatcaagaaa acatggaaac ttatgaaaac gaatattggt taaccaaatt agagaactaa
      901 aaaggagaaa aaaatatggc tcaaaattac tatgacgaat ttgtcaaatt acctttggat
      961 aaaatggcac aaaaaatgga ggatatgaca ttcctataca atgagacacg tgttccaaaa
     1021 aaacattata aggaaagatt gtcggtagct gtggaagaaa tgattgaatc gggtgtcgaa
     1081 atgaatctca ttgcgaccta ctatcgaacg cttgaagaac tgaaaaagca aaatggacaa
     1141 tggtttttcc aagccttact atgcttggaa gcaggcgtca agccaaacac tatcaaacct
     1201 tctgaatacc aagctttaga actaacgtat gggaaatttg tcgaaacgaa aaaagccaag
     1261 actgtttcaa gtgaatggtt ggactactta gaaaatgtca acaagtatgg cgcatgttac
     1321 atcatgaaaa aggaggacaa cgaaaatgaa taaggtgatt tatcaactaa caacggaaga
     1381 tgtgtcacac tttttaacaa acaaagaaca tttgaatgaa gtaatcagac ggattcaaaa
     1441 agactttagt tgtatggaag aaatgaagta ctttattcaa agagtcgaag aacaattgta
     1501 tttggaaagt gaaacgagtc aaacgaaggt tacagtcagc aataattatt tagacgtgta
     1561 taaacgtcgt cctgacacat taaaaaaagg agcggagctt ctacgactag aaacagaaaa
     1621 atatatcatg aatgtttacc tagaaaggag tgaactggtc gaaaactttg tcagctacac
     1681 gatttgcagg aaacactatt actcagaatc agaatattat tcaatttttg agagcatgga
     1741 attaatggaa gagctaaaca gcaaagatgc gacggtagac gattattacc ttgtatttac
     1801 taaatttttg acgtgtggga cggaatctat cgtgttaatg ggaatggatt atgaactcgc
     1861 tgtacccgaa gtatttttga gggaatatgt agaagaagta gataacccag gcgacgactg
     1921 gtacacgact ttaactgatt ttttagatac ctacacttac gacgatacag aagaaatcca
     1981 aaatgaactt gaccgtaaac gaatgccata tcaacgattt gaaaggaaaa aaggtcaata
     2041 accatttata ccgaaaagtg agaatgtgtg aaataaaaac aaaaaacctt gaaattaagg
     2101 gaatggttct tatttcgcac caatcgctcc aaccgccccc gccgcttagg tggtgcgaaa
     2161 taagcgagca aagcccgaaa acctaagaga aagaaggaga tttatatgaa caaaattatt
     2221 cccttattat tagtcctatt tgttggagaa atacttatgc gacttagtgc agctgtacat
     2281 ttaaacctcg cagtattttt ggtaatacta atgttttgtt tgatcttttg gcgatttgta
     2341 ttagccctcg taagtctact attcaagatg attgcagcca tatttttcat aggattattt
     2401 cgatgaaagg agagagtacc aatgccaatt gcaataggaa ataaacggtt acccgtgacg
     2461 ttggatgaaa agagacaaaa agaattgcag taaccaaagc agaagtacgg caaaagtgaa
     2521 tccaggatta tgtgtattgc gttagattta ttgattgcgc aagaaaaagc aggatttgac
     2581 ataccagcac tcaaaaagtg atcgcacttt gcgcacgtgt taatacgtgt tataattatt
     2641 ttgtaaggag gtatagtgca caatgccact tacgggaaaa gaaatgctta agttattgaa
     2701 aaaaatggct gggttgagcg aaggcaagag ggatttcatc atcatcttta taaggatggt
     2761 gttagaatca ctgttcctgt tcatgccaat caagatcttg gccgaggact tgagcgaaaa
     2821 attttaaagg atgcgggatt gaaataatct cgcattcgat taaagaaagg attgactgaa
     2881 atgttaagtg agcctttagc aaaaacctat cctgctattt ttagcccaga agaaggtggt
     2941 gggtacttta ttgaatttcc tgatgttcaa ggtgcttaca caggaattaa cgaagacgat
     3001 atatcttatg gaattgctat ggcacaagag gtgttaggta tggttttagc agattatatt
     3061 gagcatgaag atctacttcc agaaccaaca ccaatcaata agatttctgt tgaagatgat
     3121 tcttttacta ctttaattcg tgtagatgtt gccaaatatt tgaaagacac agaacttgtc
     3181 aaaaaaacct taacaattcc tcaatgggcg gataagctag gcaaaagggc tggcataaat
     3241 ttttcagtcc ttctaacaga atcaattgca gataaagcag ataacattct tcgttctgga
     3301 agaaataatt agaactcaaa agggtcagaa acttaaaaat ctttggcttc tttttttact
     3361 acaaagatgg cttctttaaa ttatttgcct gattgattta aaaaaattgc gcaaagacta
     3421 cgagctaatt aagttgctcg tggtcttttt ttatgaaaac tttttagatc
//
LOCUS       DASHFF010000133         3283 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.135, whole genome shotgun sequence.
ACCESSION   DASHFF010000133 DASHFF010000000
VERSION     DASHFF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.135"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            160..627
                     /locus_tag="U2I75_002815"
     CDS             160..627
                     /locus_tag="U2I75_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEN1697861.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            902..1138
                     /locus_tag="U2I75_002816"
     CDS             902..1138
                     /locus_tag="U2I75_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697862.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            1162..1503
                     /locus_tag="U2I75_002817"
     CDS             1162..1503
                     /locus_tag="U2I75_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697863.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(1496..1687)
                     /locus_tag="U2I75_002818"
     CDS             complement(1496..1687)
                     /locus_tag="U2I75_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEN1697864.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            1897..2544
                     /locus_tag="U2I75_002819"
     CDS             1897..2544
                     /locus_tag="U2I75_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697865.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            2804..3148
                     /locus_tag="U2I75_002820"
     CDS             2804..3148
                     /locus_tag="U2I75_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1697866.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         1204 a          451 c          645 g          983 t
ORIGIN      
        1 agaaaaagac agaatgaagg cagtagcttt ggttgaattt gctgaggcgt ataaatcagg
       61 agaactcatt tcacgtacaa aataaaaaaa gtcggattcc tccgactatg aataatattt
      121 ccgacataag tattatatca taattggggg aatcagagga tggtactttt cgatgtaaag
      181 aaatatgaga caccaagcgc gaaagatgtt gatatggaac gcacaaaaca taatgtcgct
      241 gtgtttcttt cagcatatct atcagctaga tgtagagtag ggcaacctcg agaaccaaaa
      301 gtgacagcat cttattcctt ggttccacct tctacagctg atcatatatt tgaagcagaa
      361 agaatgctga tcgataaaga agaagcacag gaagaatttg agtatctgca taaattgttt
      421 gttcgaggct attcagcgat acagcatccg cataagcctg atgtgactga aaggcgcaag
      481 aagatattct atgatcgtta tatcaatggt ctgcccattt atgtaactgc tcaaaggaat
      541 aatactagcg aagaatcggt taaagtagaa tcaaacagaa ttatcatcca atttgcttca
      601 tcgttagaac tggttgcttt caagtagcca gtttttacac tttttatacc cttttattac
      661 caatttggtt tccattttat acctttttta taccaatcac ttacctattc aacgttgtat
      721 tatgatagtg tcgaaagatt aggaaacagg atcgacaaaa taaaatgtaa gggaggaaat
      781 ctccctcatc gtttaattaa gcttcgatag gcagcagcgg aaatattaag aataaggatg
      841 tgaatttcaa ctccttctaa attgttctta ttatctatca tccgttgctg tctattaatt
      901 tatgtattgg agggtatata aaatgaagat cgcttccgaa aaaattgatc aactaacaat
      961 tttagtaaac caaagaatcg aagatattat caaagaattc gaaggtgaaa agattaagaa
     1021 cataaagctt actttaatgg atcataagtt cgaattaaat tcgacaaaaa ggaagatcgt
     1081 tgagcttata gaagaagaaa tagctctcca gatcaatgag caacaattag ctaattaatt
     1141 tatgtattgg aggggaaatg aatggaagtc gaacaaataa agtatcaagg tatggatcgg
     1201 gaacggttga ttgatattat tgagcaacaa cgtttagaat tgatccaaaa agaagctgta
     1261 tgtaaagagt acaaaaaaca tttggaacaa gtaattgagt atcattcagt agaaaagtac
     1321 agatcagtgg tgcaaaaaaa tagagcaacg agtcctgatt ctcgtcaaga tgtatcgatt
     1381 agtatcaaga cgccagtatt taattctgtt aaatcagaaa agctagaaag tatctcaact
     1441 gctttggtag aaaaagcagc tgaaatgaac tccctagcaa atgaagttag tcgtcttatt
     1501 tagtcatatc gataacttga gagtctttgg tgattccgtt ttttatagaa ttaatggttt
     1561 gttcagcgga atatttagtc aagtatgttt cgctagttgc tactacttca ttgttattgg
     1621 acttaataac gaagtaatat tggccattag ttgcttctct tataacaaag tacaaatttt
     1681 tcaccaccta taatttattt cagcggacca ctcgctgata attaaaatta taagctatgt
     1741 ataaaatttc acaatatcaa tttgtcgctg tggcggaaag ggtagacgca ataaaggatg
     1801 aatgggaaaa gtcaatgagt aattatctga aatttacaag tcccctgttt agttatctaa
     1861 gattatttga taatattatt tctgaggtga tagaaaatgg tgggcgtagt taaatttctt
     1921 gggtttacat ttgatggata tggtacgtta ggcgaatggg taggtattat tttgatagtg
     1981 attacaattc aacaaacatc aaagtactac tggtattcta atagttctaa aattgtatca
     2041 agaataactg aaggaataac caaaaaagat attactgtcg tcggtaaata tgggtttact
     2101 ataactttat taaatgaagg gaaatctgaa accgcaataa agttttgcgg gattataggt
     2161 aaatataaca ttttccaacg ttttatcaga aagcattggt acaaatatat ttacaaatac
     2221 cctgtagtta aaaagcttat tgaaaaatat gtggaccacg aaatatctta tctcaatcca
     2281 acagacctta ttaatcaaga attccagaat attggatact tagaaaaagg tctaaaaata
     2341 ccaattgtaa atgaaagttt agttactgca atggtagcta acataaagaa cagcaagaaa
     2401 ttagaaaaga gatataaaaa atgtaaaaaa ttgaaatttt cctttttatt cttagaatat
     2461 aatggcaaag aatattatca agatatttat gttttgcgag gctcgaactt atataaagac
     2521 attaaaaata agatttcagt atgagatttt ttaaaattcg tagatcactc tttgagtggt
     2581 ctttttattt tgcgtaaagg aggcacgacg atgtatagac cacaatactt agaacagaag
     2641 catgaagtaa tcactgtgca aaacggtaac ggtgagatag tacgaaagta tagaagacca
     2701 ataaagagcg atacatataa acggaaggaa agcaatgaag ttattccatt gtatggcaaa
     2761 agaatagcta agtattaaat aagattgcga aaggagatgg gacatgaccg aggaattcta
     2821 tagatggtta ttacagttga taagagaaga tcgtttggtt aagttctatc agtctcctaa
     2881 atggcgcagg cttagagaga aagcgatgaa acgagatcac tatgaatgcc aagagtgtag
     2941 aagactaggt aagtatcata gagtagagaa cgttcatcat ataaaggaag tcaaggatag
     3001 acctgactta gctttagatt tagataatct tatttgttta tgtgttgaac atcataatga
     3061 agttcatggc agatatctta cagcgttaga taaacaagag aagaagatag aaagcttcgc
     3121 taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat caatgacaat ggcagagagt
     3181 atgatacaga aaagattgaa gagtattcat cttatactca gggattaatt aaacgtttga
     3241 tatacgttcg ctatgtaggt attagggatc tgttatcaga taa
//
LOCUS       DASHFF010000134         3268 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.136, whole genome shotgun sequence.
ACCESSION   DASHFF010000134 DASHFF010000000
VERSION     DASHFF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.136"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            235..549
                     /locus_tag="U2I75_002821"
     CDS             235..549
                     /locus_tag="U2I75_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN1697867.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            527..2221
                     /locus_tag="U2I75_002822"
     CDS             527..2221
                     /locus_tag="U2I75_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1697868.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2241..>3268
                     /locus_tag="U2I75_002823"
     CDS             2241..>3268
                     /locus_tag="U2I75_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1697869.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGV"
BASE COUNT         1172 a          455 c          663 g          978 t
ORIGIN      
        1 gtgaatcaag taagagaagc gttgaataaa gataataacg tcgaaagaat aaaaaatgtt
       61 tttggatata gtattgaaga gattaattat tacattgact tcgctgaagc tttcattccg
      121 atggtgagat aaccccccct taaaataaat cgcaaatttt ttgggggtga tgaaacggag
      181 ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg aaaggagggc taaaatgttt
      241 aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac gccgctcttt aataagccaa
      301 ttggaaagtc agaaaacaaa tattgagcca ttcttagata atgttgatcg ttatatcagt
      361 ttatgggaaa cggcgatatc actggaagaa gatatatccg agaacggcat tagactggag
      421 aatggtaaaa agaatgaatc agtagcgttg cttgtttctg tcaacaaaca aatgggattg
      481 atgttggata aacttgccat tactcctgaa ttggtaggtg aagcaaatga atcaattcct
      541 gagttataag catattgaaa attggttcaa agctatagaa gaaggcacta tcaaggtatg
      601 caaagagcaa ttattgctaa aaaattatct agaagaaaga gtctttacta gagaagatat
      661 ttacttcgat aagcagatgg tagaggattc aatcaatata ccagcacaat actttccatt
      721 cgaattaatt ccgtgggaaa aatttctaca atgttttatt tatggtgttc gatggaaaaa
      781 agataaaaca ctagtgttca atagatatct ttcattaatg ggacgtggta atggtaaaac
      841 tggttttgct tcttggaaca acttctttct actaaccgct aaacacggta ttaaaaatta
      901 tgatattgat atctatgcca ataatgaaag ccaagcaaag actagttttg atgatgtatt
      961 taaagtaatt aaagatcatc ctgatttaga taaaaaagta tttaaagcta cgaaggaagt
     1021 tattcaaaat atcgctacaa acagcaaact tcgttataac acggcaaatg ctagaacaaa
     1081 agatgggaag cgaccaggtg caaaccgctt tgatgaaatt cacgaaaatg aagattattc
     1141 aatgataaat gtggctactt ctggtggtgg taaaattcga gattatagag aattttatga
     1201 tacaactaat ggtcatgttc gtggtggtcc gcttgatgac attatagaag aatcaaaaat
     1261 gattctttct ggagaacttg gaattgacaa ggatggagca gaattttcta gtttgtttcc
     1321 atttatttgt cgcttggata acgataatga agttgatgat cccgacatgt gggaaaaagc
     1381 ttgtccaact attaattaca atgcagattt aaaacggaaa atgtttcaag aatactctca
     1441 aatgcaacgt aatgctggtt taagacttac gttcatgacc aaacgaatga acagacctat
     1501 ggaagataca cgatttgctg ttgcttcata tgatgatgtt ctgcatacga aagaaaaaga
     1561 atttcctgaa aaaatggatg aagtgatagg aacagtcgat tttgctgata gacgagattt
     1621 tgccagcgtt gggttgctag gaaaatacga taaagatgtt tattttacac aacatacttt
     1681 tatccacgaa tcagcccttc gattacaaaa catcaaacga gaggttatag atatttctat
     1741 agatcaagga aaatcacaga tcgttcatgg aaaaaatata gaagctgatt atattgtagg
     1801 ttggtttctt gaaatgagta ataaatatta tattaaaaaa atcgctatgg atatgtaccg
     1861 tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt actgtggaaa ttgttcgaag
     1921 cggatctgtt acacatggta tgttaaaaga tctggttgat gaccttttta ttaatcaacg
     1981 tttatttttt ggtgacgatg cgattatgcg ttggtattgc atgaatgtat atgaagagca
     2041 tatttctaat ggaaatatac gctatgaaaa aatagaacct gaaactagaa aaacggatgg
     2101 ctttttttca ttccttcatg gtttgaattt tttagatgat atttatgatt ctgctcctgt
     2161 aacagtcaca aatagctcag tagaaaatac aggaactgga tttactcctc tagtattcta
     2221 acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc ggtaggatac ttcacaaaaa
     2281 aagcaacggt tcctttagaa gaatactttt gtaaattgca agttgatttt gtgtatcgaa
     2341 aatttgcgat tgaaacttgt attgatttga ttgcaaatgc gatgagtaaa gcggaattca
     2401 agtcatatga agatggaaaa aataaaaaga atgatcttta ctataggctg aatgtagctc
     2461 ctaataagaa aaataatgca acagaattta gaaaaaaact gatcaggaga ttaatattct
     2521 acaatgaagt attgatcgtt tctccgtcta ataattctag cgaaatattt attgcggata
     2581 gttgggatgt cacagaatat gcattgaaag atgatgtgtt ttctcaagtg caaattaaca
     2641 acatagtcct tgatagagaa tttctagaaa gtgatgttat ctatataaaa tacgcagatc
     2701 aacaaattag gcaactagtc gatgcgtatt atcaagcgta tgggaaactc atttctagtg
     2761 ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt actgaaaggg aatttattcc
     2821 gaccgcaaga caatacaaca caagatcaaa tcaataaaat gatgacatca caatttaagg
     2881 cttttatgga agctgataat gcaggtgcgg tatttcaatt acaaaatgag tacacattag
     2941 aagatttcag cggaaacttc caaagcaatt caagagatat aaaaaactta atagacgaca
     3001 tctttgagat gacagcagca gcgtttcacg ttccgaaaaa cctactaaag ggagacatga
     3061 gtgggttatc ggatcaagtg gacgcttttt taatgttcga aatcataccg attgctgaac
     3121 ttattcagga tgcgtttaac gctagtctct atgaagtaga agaatacttg tcagggaatt
     3181 ttgtacgtgt ggatacaact atgatcaaga ttactagctt caaagatttg gttgacgcta
     3241 ttgatgtagg cattagaaat ggagtatt
//
LOCUS       DASHFF010000135         3217 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.137, whole genome shotgun sequence.
ACCESSION   DASHFF010000135 DASHFF010000000
VERSION     DASHFF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.137"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            49..1437
                     /locus_tag="U2I75_002824"
     CDS             49..1437
                     /locus_tag="U2I75_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEN1697870.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1775..>3217
                     /locus_tag="U2I75_002825"
     CDS             1775..>3217
                     /locus_tag="U2I75_002825"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEN1697871.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKTLLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1184 a          505 c          581 g          947 t
ORIGIN      
        1 tcaaaaaaaa accaaccgaa agatataaaa gatattgtag atacacgaat gtataaagaa
       61 cgtgatgact taaactatac gcttattatc aataaagatg gaaactattc atggcgtcct
      121 ctcgttctac tacatcctat tctatatgta gatttggtta attgtctcac taaagaagac
      181 cattgggagg agttaaaaaa gaggtttagt gaatttaaaa aagataaaaa tattaactgt
      241 tattctatac ctgttgaagc ttccaaaggt tcaaaaagga cagatcttgg tgaaacaata
      301 ctaaactggt gggaatattt tgaacaaaaa agcttagctc agaatattaa ataccaatat
      361 tgcatgttta ctgacatcgc tgattgttat ccatcaattt atactcactc tataacatgg
      421 gccatgtatg gtaaagaaaa agttaaaaat gatgaaaata agtgttcggg ctcacttggc
      481 gataaaatag ataaaaaaat atctggaatg caacacaatc aaacaaatgg tatccctcag
      541 ggttccgttc ttatggattt tgtagcagag atcgttctag ggtatgttga tttgagatta
      601 tcgaaagaat taaaaaaatt agaaaattta aaagattaca aagtactcag atatagagat
      661 gattatagaa tattttcaaa taatcgtaat gatttggaac aaattatgaa agtgttatca
      721 gaagtcttat atgatttaaa ttttaagtta aattctagta aaacaaaatt atcagatgat
      781 attatcatag attctatcaa aaaagataag ctctattggg aagaaattaa ggcaagtata
      841 agaatgaatg ccacaaactt agatggtgat agagaaattc gttatagtat aaatttacaa
      901 aaacatttat tggaaataaa aaagttagca gataagcatc ctaattcagg ccaattgaaa
      961 aaagcacttt ctgagtttaa taaagaacgc attattaaaa tcacttcaaa gccgaacgat
     1021 cttttaccac ttattggtat cctggtagat atcatgataa ataacccggg ttctttgccg
     1081 cagtgtgtct taattatagg gcagttatta aatttcgaaa atgagagtaa ggctagagaa
     1141 atagttgaaa tgattataaa caaatatgct ttaaagccaa atactgaatt tatgatgata
     1201 tggtttcaaa gattgaccac catttttgga gaaatgtttg atagtgtttt atcatccgaa
     1261 tttgtatcta aaatcaaaga tcctgtctct aatcagatgt tttctgtaga ctggctaaat
     1321 gaagatgcta agaaatcttt ttatgaaggg accattaaag acgaagagaa atatattaaa
     1381 atgagtaaga aaatatatat tactgaattt gatgatttta atgattatcc tttttaagtg
     1441 aaaatttaga atgaatataa aaaacattac aatctaattt ttttaaaaaa atgttatatc
     1501 ggaagtaatc tcttgatttc tatactataa gataagaaaa ccactattta acaaggttat
     1561 tcgaacctta ttttaagtta cttattattt aatgtatcga aaaaggtgaa ataggtttgt
     1621 tataataaga attaagtaag acatgaattg tctaagcaaa tgtagcttat ccatttcatt
     1681 aaaatacata tggtgaaaaa acaagttcaa atagtaacta attttttaaa aaactgattt
     1741 ataaaaaata caacacgaat gaggaaaata accaatgaca gaacaaatcg aaaaaaattc
     1801 aaaaagctta tatcaggcac tgtggaattc cgcagatatc ctgcgttcaa aaatggacgc
     1861 taatgaatac aagagctatt tactaggctt ggtcttttat aaatacttat ccgacaacat
     1921 gctgcggtat gtctccgttc tattggagga agaaacggac gacctaaata aagcacaaga
     1981 cctttacgtt gaagcattta aagatgcaga tataaaggat gatctcttgt atgaactaaa
     2041 agatgagttt tcatatacca tcgcacccgc attgaccttt accgcgcaag tggcagccat
     2101 tcatgatgga tcttttcaat tagaagacct ggtacaaggt ttccgtgaca ttgaacaatc
     2161 cagcgaaaac tttgagaact tatttgaaga cattgatctg tattctaaaa aactggggtc
     2221 aaccccacaa aaacaaaata aaaccatcgc agatgttatg aaagaattag aagggttaaa
     2281 catggcgggg catgctggcg atgtcttagg cgatgcctat gagtacttaa ttggccaatt
     2341 cgcatctgaa tccggtaaaa aagccggtga attctatacc ccacaaccgg tcgcaaaatt
     2401 gatgacacaa attgttttac aaggaaaaga agataaaaaa ggcttttctg tttacgacgc
     2461 gaccatgggt tcgggctcgt tacttttgaa cgctaaaaaa tattcccatc aaccgggaac
     2521 catttcttat ttcggacaag agttgaatac gtctacctat aatttggcgc gtatgaacat
     2581 gattttgcat ggggttccca ttgccaacca acacttgcat aatgcggata ccttggatca
     2641 agactggcca acagaagagc caacgaactt cgatggggtg ctcatgaacc caccgtattc
     2701 tgcaaaatgg tcggctgata aaggcttctt agatgaccca cgcttctctg cctatggcgt
     2761 attagcccct aaatcaaaag cggactttgc tttcttatta catggctact atcatttgaa
     2821 agatacgggc gtcatggcca ttgttttgcc acatggggtg ctctttagag ggggcgcaga
     2881 agggaagata cgcaaaacct tgttagaaaa tggtgccatt gacaccgtca ttggcttacc
     2941 tgctaacatc ttctttaata cgtccattcc aacgaccgta attatcttga agaaagaccg
     3001 tgcgaaaaaa gatgtgctct ttattgatgc gtcacaagat tttgaaaaaa taaaaacgca
     3061 aaatacgcta cgagattacc atattgacgc tattttagag gcctataaaa cccgtacaat
     3121 taaagacaag tatgcttatg tcgcagaatt tgatgaaatt gtggagaatg attacaactt
     3181 aaacattccc cgttacgtgg atacctttga agaagaa
//
LOCUS       DASHFF010000136         3199 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.138, whole genome shotgun sequence.
ACCESSION   DASHFF010000136 DASHFF010000000
VERSION     DASHFF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.138"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            668..763
                     /locus_tag="U2I75_002826"
     CDS             668..763
                     /locus_tag="U2I75_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1697872.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1065..1310)
                     /locus_tag="U2I75_002827"
     CDS             complement(1065..1310)
                     /locus_tag="U2I75_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697873.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1655..1939
                     /locus_tag="U2I75_002828"
     CDS             1655..1939
                     /locus_tag="U2I75_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697874.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1942..2358
                     /locus_tag="U2I75_002829"
     CDS             1942..2358
                     /locus_tag="U2I75_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697875.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2495..3172)
                     /locus_tag="U2I75_002830"
     CDS             complement(2495..3172)
                     /locus_tag="U2I75_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1697876.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag
      241 ggttctaaaa aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc
      301 cgccaaaaaa tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa
      361 tctagctccc tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc
      421 agcagtcgcc tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc
      481 ctgagagctc gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct
      541 aaagcgcctt acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag
      601 gcttattttt gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg
      661 gaaggaggtg tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag
      721 cgctttgttc agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac
      781 tcagcccaca aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg
      841 ttcacttatt tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca
      901 aaagtctaaa atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag
      961 agctcgtatt tttcgattta agacgtttta tttagttttt ggtgtatttc attggtccaa
     1021 gctaaaaaac tcttagaagc gattttagag ccatttaaac ccacttaatt atattctcca
     1081 tgaaattcgc tccatgcgtc aaaagctgcg tctgaaattc cgccgtcttc gttcactagt
     1141 tgcattaatt tttccaacga tcgttttctt tcgggtgtca tatttttata ttccaacaat
     1201 ttttcgtctg ttaaatcgaa cttgattgct tgaattgctt gctctaattt ttctaagttt
     1261 tgaaaatatt cgtaaggaaa tttttccatt ttttctttct ccagtctcac aatttttcta
     1321 gaaataaaat cacttctttg ccgtctatac tctcaaaaaa aatgtcgttt gaaaaactaa
     1381 ataaattttt gatcgtgcat ttttaaattt ggttatcgat tacacgtgca gcactccccc
     1441 ccgaatttta aagagcataa ggaaacgtgt acccactttc taaaaaaagt gggtagatag
     1501 gctgtgccta tcacgtttct cagaaaaaaa tttgatatca ctttttcgcc atcgtgggta
     1561 gcttatgtat agcattttgg caaaaaacag ctatacaaat atagtatttg tatggtatac
     1621 ataaattata cataattgca aaaggagatt ttttatggac attttagttc ggaatattga
     1681 tccaaagttt gttgcaacaa tagacaaacg ttgccaagaa ctcacgaata aaacaggaga
     1741 aaaatggagt cgaaataaat atctaaaagt tttaatcgaa agtgattttg accgtcaact
     1801 tacgacttac aaaaaagacc aattcgattt attgctcgat aaatttattg ctatccaaac
     1861 agaaaatact caaatattaa gcgagtatgt ctacacaaat aatcaaatca ttgaaatgct
     1921 tatcggagga atgatataac catgagaaaa acttctgtcc aaaaacatgt tactttgtac
     1981 cctagtcatg ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac
     2041 tcggaagcca ttcgcttttt atgtatgagt tatgaacccg ataccaacac gaccactacc
     2101 agcaatcagg caaaactaaa tgctatggga aaagaaattt ccatcatgac tgaaatcatc
     2161 gcagaatttg ctgatttata tctaaaagat acaggcgttc ttattggcac aaattctaaa
     2221 gtatatcaag aagtgaaaga aaaaatcgaa gctaaactaa atccatcaaa tcgaaatcga
     2281 ccaaagaatc atcaaaaaga aaccagtcaa acagtcactt ctagaaattt cgacccaaca
     2341 aattttctaa aacgataaaa aacagatagg agttattcct atctgttttt tatttagaag
     2401 ccccatacaa tatgctagaa tgtactagca atcttagtcg gttgtaaggg acttgtctac
     2461 ttgcttaatg taaggggggc gagttctttt ttatttattc ttctaaccag ttacgcagag
     2521 aaatctttgg caaatctgac gttaggcttg aaaaagtaaa ttctaatttg gcgattttat
     2581 tgcccttttt tgcttttatt tttgtgactt taagatcatt gaagtatttt tttagttctc
     2641 gtaatgcagg atttaatact ttttgatcaa tgttacccat ttgatagtat tctgggatat
     2701 ccattaagct cctaaactct tcaatacgaa ctacataata accagtagaa cggtactgca
     2761 taagcagtcg gaatagagtt tttgcatacg tagaacgcaa cgacgtgaaa gcagataatt
     2821 caaatctact aaattcagct gttaaaccat taataatata ttctaggtcc ggattaacac
     2881 tgacttcaac aaactttttt gttttattga tttcaaaacc agtaaacaaa acaaagtagc
     2941 gaatagaatc ctcatcttca gtacggtaag taagattaag catatcttta tagagctttt
     3001 ctaaatcacc gacaaaggct tttgtagcag tcatcttata atcacttaac tcttttaatt
     3061 cctcgaaaga aaatctaact tttttcaaac cacgatcttt catacgagcg cataatgcaa
     3121 aaaataaatc catctgctta gcattgaaat ttttcagcgg tactaaattc aattcatttt
     3181 gatatttgac tgttaattc
//
LOCUS       DASHFF010000137         3091 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.140, whole genome shotgun sequence.
ACCESSION   DASHFF010000137 DASHFF010000000
VERSION     DASHFF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.140"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            256..492
                     /locus_tag="U2I75_002831"
     CDS             256..492
                     /locus_tag="U2I75_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697877.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            499..2451
                     /locus_tag="U2I75_002832"
     CDS             499..2451
                     /locus_tag="U2I75_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1697878.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2523..3020)
                     /gene="dfrG"
                     /locus_tag="U2I75_002833"
     CDS             complement(2523..3020)
                     /gene="dfrG"
                     /locus_tag="U2I75_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1697879.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1092 a          513 c          548 g          938 t
ORIGIN      
        1 agacaacgtc ttgcatatta agtagttcag ttttggagta caaaaagtga aaatgtaaac
       61 ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt aaatcaacgt
      121 ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg cgaacgtgaa
      181 ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac taagcaaaaa
      241 ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa gtctgtggat
      301 tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata tgcagagcgt
      361 actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa gaagtttatt
      421 gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg tatcaaagat
      481 agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga agatgaaatt
      541 gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa acaatatgat
      601 ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt gccaacgtct
      661 tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa tccgttttgt
      721 tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc aagtcgatat
      781 aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa tgacaatcct
      841 attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg gtctgttgtt
      901 gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc agattatcaa
      961 tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc gaagcaattt
     1021 tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc gtgtaagaag
     1081 ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca aaagaacacc
     1141 attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg tacttgtgat
     1201 attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta taggcgttgt
     1261 ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt taacgagatg
     1321 tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa aggacaaggt
     1381 tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt tgtatctgct
     1441 gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa tatttcaatg
     1501 gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac ttttagtcgt
     1561 aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa cgatagcgaa
     1621 aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag atttgtcgat
     1681 ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat taaacaaatg
     1741 gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag aaacgccttt
     1801 tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat ttgccaagtt
     1861 aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa agcagaactg
     1921 ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt ggcatctcat
     1981 tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag taaagatgga
     2041 gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac taaggataaa
     2101 ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa cgaaatcgca
     2161 aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca gataagacgt
     2221 tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa gagctatatt
     2281 tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag aacttactac
     2341 aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc tcaaagatta
     2401 gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg acaaaaagag
     2461 cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa ttaacgcacc
     2521 cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata cggattttta
     2581 tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt cacttctgga
     2641 aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt ctcaacataa
     2701 gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta
     2761 cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc
     2821 ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg
     2881 ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc
     2941 ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt atccatcgca
     3001 gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc atctaatttc
     3061 ttatttctat caaaaaacct tttctcatta t
//
LOCUS       DASHFF010000138         2992 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.141, whole genome shotgun sequence.
ACCESSION   DASHFF010000138 DASHFF010000000
VERSION     DASHFF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.141"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..861
                     /locus_tag="U2I75_002834"
     CDS             <1..861
                     /locus_tag="U2I75_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697880.1"
                     /translation="HIHIHFAVVEKENTRKPYTEIENGVSYIVPKGKRRQATLDAMKT
                     NYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYDRHAMNLLNEVYK
                     TLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQISKALKEEAKSL
                     YGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVNQMQSIYNERSFR
                     TLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEEKYHASKAYEEQQ
                     RRIQMEQERQ"
     gene            873..1208
                     /locus_tag="U2I75_002835"
     CDS             873..1208
                     /locus_tag="U2I75_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697881.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            1355..1549
                     /locus_tag="U2I75_002836"
     CDS             1355..1549
                     /locus_tag="U2I75_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697882.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            1581..>2992
                     /locus_tag="U2I75_002837"
     CDS             1581..>2992
                     /locus_tag="U2I75_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697883.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEV"
BASE COUNT         1223 a          454 c          629 g          686 t
ORIGIN      
        1 catatccata ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc gtatactgag
       61 attgaaaatg gtgtgagtta tatcgtaccg aaggggaaga gaagacaagc aacattagac
      121 gcaatgaaaa ccaattatgt acatgagtta gaacgtttct cttatgaaaa ggatcgaatg
      181 atcggtatgg agaaacgaaa tttattgaca cgaaaaagtg atttgcgtaa caccttaaca
      241 aaagtagtaa aagaaccagc acgttacgat cgacacgcca tgaacttatt aaatgaagta
      301 tataaaaccc tacctgaaaa acgttcggaa tggaattatg gagatgaaaa tcgaaccaag
      361 ctttcaccat ttacaagggg aaaacttgat gagttaacca aatatgtttt aacaaaagaa
      421 cctggatatc aagaatatat acagatttca aaagcattga aagaagaagc caaaagcctt
      481 tatggggaaa gcaaaagaga aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa
      541 aagcgcacag gaaatgcgat tttaacagaa ctgaagagac aggatagtca aataaaacca
      601 ttaatagaaa gtgtcaatca gatgcaatct atttacaatg aacgatcctt tcgaacactc
      661 aaacagttgc caacaaatga atacaagtat agttatcaag aattcgtaaa gcgtaggaaa
      721 caattccaac aaagagttgt cacgcagcga agcttgcgtc aattaaatcg aacaattaat
      781 gcacatgaag aaaaatacca cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa
      841 atggaacaag aaagacaata ggaggaagag aaatgaaaca atatttagat caatggaaag
      901 tcatagaagg atcactaaaa gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag
      961 agcatttgtt tcagataagc gaaatgttga gaaacgaaca gtttgatcca aatcaatttt
     1021 tagttgttga gtacccagct actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat
     1081 attttattat tcaagaatac gaaggtaagt tagcgcctta ttatactact tgggaaataa
     1141 atgaagaggg aatcaataat ttcccatgtg aaagcattga agagtctatt tctctcacgg
     1201 aatgttaaat aaatagagac gtaaaagagt cctgaacgtg attgtccagg actctttttc
     1261 aatgaattgt tttccgccaa ttcactgagt ggtgcgattc aattttatta tatcaaaaga
     1321 aagtgagatt ccaaagtgaa gggaggaaga agaaatgact cagacaacga ttggtgttgt
     1381 ggttgaaaaa agcagggata atctcgtttt tgtgacagaa attcaaacag gtagagcttt
     1441 tgtagtcaca gacaaagctg caaaagcgta tcaaaatggc aatattttaa cgttaaatct
     1501 gactacgaag atgttcgttg atgcagtaga aaattatcca tttgtttaga caaacgatat
     1561 tttacaagga ggaaacggac atggcagaag aaaaagaacc actagataat agtcggttgg
     1621 gaaagtccaa aagaaaatta gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc
     1681 tatttgatca ccaaaaattg acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt
     1741 catggtttaa ccagcaagaa aaaatagaaa ataaaataca aagtttgaga gaagagatca
     1801 agcaacaaga aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggct
     1861 ataacaagta cggcggactg gatatgacga tcgataatat tccaagaatc aaagaagaaa
     1921 tcgaacgctt tgaaaaaggt gaatcgactt tttcagccgc cacgatccga aaatatcaac
     1981 ggaagttgga aacattagaa cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc
     2041 tccctgaagt acaagcgatc attgacagcg gacgagtgac gcaatggaaa aaaaatccaa
     2101 ccatttattt tttgaaaggc tatcgaaaag ttgctttaga actgaatgaa aacgggggat
     2161 ttgaggaatc aaaaaaatac caagccacgt cggaagagga aaaagcagct gttcgtgaat
     2221 tacttgaaga ggctgcacca actgattctc tgaaggagaa agaaaaagat catttctatc
     2281 gagtagaatt caacgaaaat gatccagaaa aaggattgaa aagttatggt ggggagatcg
     2341 taacattcga attgatcgat caacttaaaa cgttggataa ccaatacgct gcagaagcag
     2401 gttattataa attctatttt gaagaagtga agaacggaga agtagtagat cagatccgaa
     2461 tggatctagg ggatggcgtc aacagtaatc accccatcta tttagagtta gcaaaattgt
     2521 gcgaacaagt acctgagaag gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag
     2581 aacaaatgca gtcgaaagaa acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag
     2641 aagagatcaa acaagaagtt aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc
     2701 atacccattc ttctgaagac gtcaaagaac ttttgaactt tatgtctcgt tttccagaac
     2761 gatcctttag aaatcaatta ttgattgagg aacaatttcc aggtgcaacg gcttgtttag
     2821 gtcgtgccgc gttgaaaaaa gaaggaatct atatcaaaaa aggggaacaa gggaacaaga
     2881 tctttgtcag aaaaacggtc aaaggatttt atgagaaagg acgtggcttt gttcgagaaa
     2941 cagaagctac accagaagat aaaaaacgaa tcgaaagtgg tcaaatcgaa gt
//
LOCUS       DASHFF010000139         2785 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.144, whole genome shotgun sequence.
ACCESSION   DASHFF010000139 DASHFF010000000
VERSION     DASHFF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.144"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(606..962)
                     /locus_tag="U2I75_002838"
     CDS             complement(606..962)
                     /locus_tag="U2I75_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN1697884.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /gene="spx"
                     /locus_tag="U2I75_002839"
     CDS             complement(976..1386)
                     /gene="spx"
                     /locus_tag="U2I75_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1697885.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            1605..2342
                     /locus_tag="U2I75_002840"
     CDS             1605..2342
                     /locus_tag="U2I75_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697886.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKG
                     KRIMVPLPVQKNVLSIKSEDVFLDFFF"
     gene            complement(2325..>2785)
                     /locus_tag="U2I75_002841"
     CDS             complement(2325..>2785)
                     /locus_tag="U2I75_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1697887.1"
                     /translation="DLDALQVVSVVSFYESFIDGADPLDMQGYLQTQLTTEILAVELA
                     QSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLGQRLLDEANRYAKT
                     KQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPILEKEI"
BASE COUNT          861 a          501 c          438 g          985 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa aacccgtggt gctagttaat tttttcctag
     1501 aggaaaatgt ctaaacatgt tggtaaatca acaaaaaaac gcatatagta gtctcaaaac
     1561 tcccaagaaa tgaggactat ttaacaaact acgatggtta cgtcgtgaca actgaacatt
     1621 ttgacttagc caatcctgtt actaaagtcg atgatatccc cgattatgaa atgtacagtc
     1681 aaacaatcga tagcttaaat aagcgttttg gcaatcgtgt tttaaaaggc atcgaaatcg
     1741 gttacatcgc ttctgaaaag gatcggatca tcgactattt agccgataaa gattacgatt
     1801 taaaattact cagtgtgcat cataatggtc aatttgatta tttagatgac gaagtcaaag
     1861 atatggatcc tgctattgtg attcctcagt attttgcaca actttcagaa gcactagttg
     1921 ttatagaagc agatgtcttt gctcattttg actatggttt tcgggtattt ggtctatccg
     1981 ttgctgaatt taaacaatat gaagcgcagt ttttaccgat tctagatcaa gtaattaaaa
     2041 ataaattggc attcgaatta aatgctaaga gcgcttactt gtatgacaat ttagcgttat
     2101 atgaatatgt catcgattta tatctttcac gtggtggtac cttgtttagt gttggatcag
     2161 atgggcatta ccttgaacat ttccgtttcc atttcgatga tctttttgct ctgttaaaag
     2221 caaaaggtgt gacggaatta gcgatttatc aaaaaggaaa gcgaattatg gttccattac
     2281 cagtccaaaa aaacgtcctc agcatcaaaa gtgaggacgt ttttttagat ttctttttct
     2341 aaaatcggac aaatacgatc ttgttttcct aaaggaaaat gtttgatccc gattttttta
     2401 aatccatgac gttcataaaa acgaatacct gcatgatttt tttcataaac agtcaaacgt
     2461 aaataacgtt tttgtttcgt tttagcataa cgattcgctt catcgagtaa ccgttgccct
     2521 aaccctttgt tttgataatc tttgagcaga tagagccgtt gaatctctat agcatctttt
     2581 tcatcattga ctttcatata accaatgagt atttgatgat ccttaatacc aataaattta
     2641 ctggttgact gtgccaattc tactgccaat atttcagttg tcaattgtgt ctgtaaatat
     2701 ccttgcatat caagtggatc tgcgccatcg ataaacgatt cataaaaact gacaacactt
     2761 acaacttgta aagcatctag atcat
//
LOCUS       DASHFF010000140         2730 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.145, whole genome shotgun sequence.
ACCESSION   DASHFF010000140 DASHFF010000000
VERSION     DASHFF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.145"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(40..1416)
                     /locus_tag="U2I75_002842"
     CDS             complement(40..1416)
                     /locus_tag="U2I75_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1697888.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1544..2662)
                     /locus_tag="U2I75_002843"
     CDS             complement(1544..2662)
                     /locus_tag="U2I75_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1697889.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT          854 a          557 c          437 g          882 t
ORIGIN      
        1 tttattcaag caacacgtct ttcataacca agtttccaat tatcttcttg gctcgtcatc
       61 ttcgaattct tttctgaagt atcttcgttc ttcttcaggt tccgcttctg tttcttccgg
      121 atcatgaaaa atgacacgga ttttaaggat tcgagaacct tccatttctt cagaaaccag
      181 cgtaatatta tcgacatcaa acgataactt ttcgccttca tccggaatca tgcctaatgc
      241 tgtaatcaaa taaccagcca tcgtatctac atcactcata tgcagatcac tgtcaaaagc
      301 ttcattgaac tcatcaatca gcatacgtcc ttggatgata tattcgtgtt catcgatttt
      361 ttcataaaga ttctcaactt catctgattc atcatcgatt tctccgacga tttcttctaa
      421 aaggtcttct agtgttgcta acccaacgac gccgccatat tcatctaata aaatagccat
      481 ctgattctgc gtctttttca tttcatataa cagatcatca ataaagaccg tttcaggaac
      541 gaataaaggc tcctgcatga ttttcttgat atctaaatta tcaaagccga atttatgggc
      601 agcttttagt aaattctttg tatgaaggat tccgactact ttatctttgt cttcgttata
      661 aacagggatt cttgaatagt tttcggataa aacttcattc acattttctt ccacagtgtc
      721 gttgatatca atcataaatg catccgttcg tgggaccatg acttctcggg caactttcgt
      781 atcaagagaa aaaacccctt ggagcatttc taattcttca ttattcaata cgccttcgtt
      841 ttcaagcata taacgcattt catctctggt catttttgaa tcttcatcat caaaagtcat
      901 cggcgtgata cgcgccaata aacttgtgga agcagaaagc agccagacga agggcttagc
      961 tactacacca atcaagcgta tcagccctga agtgaacttt gcgacttctt ctgatttatt
     1021 taaagcaata cgtttagggt acaattctcc aaaaacaatc gatacatagg tcaaaatagc
     1081 cagcacaatg atatttgcaa tattttttgc cgctgtgccg ccacctaata ccggagctaa
     1141 ccgttccgat aaagtattag ctagtgaagc accagacaaa atgttgacca aagtgattcc
     1201 tacttggatt gtcgatagga aattatttgg attctgcaat atttttaaaa gcttttgaga
     1261 tttcacatct ccttcatcag ctttttgctc gattcgattc ttattgatcg aaacaacagc
     1321 aatttcggaa gctgctaaaa aagcattgat caatgtcaaa acgatcaata ataaaatctg
     1381 cgctaacagc gactgactct cagggtcagc attcataatc gcattctctc cttataaaat
     1441 taatttttag tttacgatag aaaaaaacgt ggcactatag ccacgttaaa atggtatcac
     1501 aaagcaattt tctttgcaat aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa
     1561 ttttcttctt gttttcattt tgcttgtctt ctttgacgat tttgattata taggaaatga
     1621 tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat accaagcaat ccggcaatat
     1681 ttccagctac tagaagaacg ataatgatcg ttaatggatg gattttcagt gatttcccaa
     1741 ttacattagg ataaatgata ttcccatcca actgttggac gattagcaca accacacaac
     1801 aaagtaatgc ttttaccgga tcattgaaga ctgttactag aaatgcaggt gctagtccta
     1861 gataaggtcc taaatatgga attaggtttg tcaatcccgc aatgacccca aataagaaag
     1921 cataatccac accaattacc atatatccta aaaatgtgaa ggtaccaacg aataaacatt
     1981 cgattgcttg tccgctaatg taattagcca atgttttatt cagttgcccc aataactcca
     2041 caatttggtt tcttcttttt tctgggaaaa actgttggat gtttggcact agtttttctc
     2101 cgtctttcaa catatagaac aagatgaatg gtgctgtaat gattactacg gttgccgaag
     2161 cgatcgttcc aacgatcgaa cctaaactat tggatactcc actaaggaat tgctgaatga
     2221 ttgtcccgta agaaatgtct aattgctcaa aatatttatt gatatctact tccttgaaca
     2281 aaggcaattc ggctacctct tttaaccagc tttctacctg tttgatgaaa tcaggcatat
     2341 tagatgccaa tgaggaaatt tgctgtacta gacttgggat gacactaagt aaaatccaaa
     2401 taattgctgc aactaaaagg agcagcacaa ttgcgacagc ccatattctt ttgatttttg
     2461 ctttcatcaa cagcttgaca ataggattca ataaatagta aagaaagcct gcaatcagta
     2521 caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag tttatttctg
     2581 atgaaatcat gatcaatgca gctatgatca ataattcaag cgaccaaaac attagtttag
     2641 actgtctaag tttttcaaac aatagatttc ctcctttctt tcttactagt atatattata
     2701 ttcgttaaaa attcttttat tcttttttta
//
LOCUS       DASHFF010000141         2693 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.146, whole genome shotgun sequence.
ACCESSION   DASHFF010000141 DASHFF010000000
VERSION     DASHFF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.146"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1444
                     /locus_tag="U2I75_002844"
     CDS             <1..1444
                     /locus_tag="U2I75_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697890.1"
                     /translation="SFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCW
                     LPYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVF
                     LFSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFS
                     IACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIIL
                     KQKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLS
                     KKDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIK
                     IYTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDAR
                     DFFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLA
                     LGLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            1431..2633
                     /locus_tag="U2I75_002845"
     CDS             1431..2633
                     /locus_tag="U2I75_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697891.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT          770 a          481 c          465 g          977 t
ORIGIN      
        1 ttctttttta tttttacaat cgatttattt atcaatgcca aaactggaaa cattttttac
       61 tgctcaaaac acacaggctt tctcttcaag aaaatatttt ttattcttta ctgtcttttt
      121 agttctgtgt tggcttcctt atctgttggc attgtatcct ggagtggttt tgcctgattc
      181 gttaagttcg gtcgcccaat ctatgggtga tgttcctata agcaatcatc atccagtctt
      241 attcacctta ctggtcaagc tatttgttca tgatttacca actgatacga tcaatcacgg
      301 tgtattttta ttttcgattt ttcaaacatt gataacagct tcagcgataa gttattctct
      361 ttgctggctg ttaaaaagaa acatccattt ttttccggta ctcattagtt tatgctattt
      421 tgcatttgca ccggtttttc caatctacgc cttgaacatg caaaaagata ctttgttctc
      481 tatcgcttgc tttttatttt ccttagcggt ttttgacttt atttttcaac gagaaaagcc
      541 aacgattctc aatcagattt acgtaatcat ccttgcttta ctgaccacct atctaagaaa
      601 taatgggatt tatgtagtaa ccggcacagg tttgatttgc ggcttgatta ttttgaaaca
      661 aaaaaggtat caagcattct ttcaagtggt gctgggttat acagtgattg ctggactact
      721 catccacccg ttgatggctc gttatgctat tcctacagaa actgctgaac aattaggtgt
      781 tcctttgcag caaatttcta gaactattgt tatggatggt gacttgtcta agaaagataa
      841 agcattcatg aatcagctac tcccgataaa agattatgaa gcgtattctc ctagtcttgc
      901 agactcaatc aagtggcata aaaattttaa caatgagttt ttgaatacac ataaaaaaga
      961 atttctatca ctatgggcaa gaaatttacc aagtaatata aaaatctata cagatgctta
     1021 tattttagaa acgttcgggt tctgggtacc tggtacaaaa aatagttacg gctttttaga
     1081 tactcgtgta aacacgaata attatggtat gaagcaagta gatttgattg aacatcttac
     1141 tggattgtca ggaatgaaaa aaatcatcga tgctcgtgat ttctttggtt ccggcacatt
     1201 actgtggtta gctatcggca gtgcactttt agcaatcttt aagaaaaaat atcggtattt
     1261 actccttttt cttcccggta tgcttgtttt tgctactatt atgattgcta ctcctgtggc
     1321 ttttagttta cggtatgtgt ttgttttagc attaggtcta cctgtttata tcctctctcc
     1381 ttttttacta gataaaagag aaaaaatgaa aacagatata ggagatactc atgaaactat
     1441 ttaaaaagat attttttgct tttgttctct tgatcgctag ctattgcgta ctactgacaa
     1501 ttactttttt cattccgcag cactctattg aatcgaatgc agaaaaatct ctacagatgg
     1561 ttgaaaaaga aggaatgtat ccttctatca atcaaggaaa tatgctaggc actcggttag
     1621 ataattttac tgatcatttg atgatacgaa aaacaaaagc agatcctgaa ttgaactcct
     1681 tggagaacgc gatgtctatg gcggattatc cccgttattg gcatggttat cagctttttt
     1741 tacgaccttt actgctaggg atgagtttgg ggagtattcg aatgatttac gcagccattc
     1801 tttttctgct gattggattg acgagctact ttttaaccaa gtgtagcgac atctatttaa
     1861 cgacagcctt gttgatttcg cttgctatag gaaatgctgc aacctttttc ttcagtatgc
     1921 aattttcaaa tatttggatt ttgactttat tgtctgtatt actattttta acaaaaccac
     1981 aactctccaa caccaaccaa caattattct tgtatttctt tgtcattggt tctttagcta
     2041 acttttttga cttgttgaca acaccggtca tctcttgggg gatacctgtg atcattattt
     2101 attatgtaac aaataagtat ttaatggata aacgcagttc attgagcaaa caagtaggca
     2161 gttttgtttt taccggtatc ttttggggat taggctatgg tttgacatgg gtggctaaat
     2221 ggatcctatc atcgatcata ttgaacaaaa acattgtaaa agatgcaatc aaccaaatcc
     2281 tctttcgaac ggaaggaaac gacaaatacc cgttgcatcg attagagatg ctgaaaagca
     2341 atattagatt gatgtatcca aaagtagcaa tattattgct attgattact tgtcttgttt
     2401 ttctctattt tgcttatcgt aaacgaaata gctggcgtcc gttccaactt ctagttttgt
     2461 ttgttttagc tgctgtaacg ccgtatattt ggtacaatat tttagctaat cactcacaga
     2521 ttcattattg gtttacgtat cgtacgcaaa tcatcacaag ttttgctata cttagtgctt
     2581 tcgccttttt gatccctcct gagaaatcaa aagacgaact aaattacttt taagaatacc
     2641 gatgtactaa ttcattttta aacataaaaa ctccagctgt cagagatagc cgg
//
LOCUS       DASHFF010000142         2435 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.148, whole genome shotgun sequence.
ACCESSION   DASHFF010000142 DASHFF010000000
VERSION     DASHFF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.148"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..382
                     /locus_tag="U2I75_002846"
     CDS             <1..382
                     /locus_tag="U2I75_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697892.1"
                     /translation="RITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDNPYSQFLI
                     TVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMT
                     VNQICEITNVSRASLYRKLSEVNN"
     gene            596..1291
                     /gene="vanR"
                     /locus_tag="U2I75_002847"
     CDS             596..1291
                     /gene="vanR"
                     /locus_tag="U2I75_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN1697893.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1269..2423
                     /locus_tag="U2I75_002848"
     CDS             1269..2423
                     /locus_tag="U2I75_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN1697894.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          839 a          437 c          515 g          644 t
ORIGIN      
        1 tcgaatcact cgtagtacac aagatctatt tgaattaatc gataacatac gagataaaaa
       61 ggcaagttta aaatcactaa aagatacatg gcttgattta tcagaagata atccatacag
      121 ccaattctta attactgtaa tggctggtgt taaccaatta gagcgagatc ttattcggat
      181 gagacaacgt gaagggattg aattggctaa gaaagaagga aagtttaaag gtcgattaaa
      241 gaagtatcat aaaaatcacg caggaatgaa ttatgcggta aagctatata aagaaggaaa
      301 tatgactgta aatcaaattt gtgaaattac taatgtatct agggcttcat tatacaggaa
      361 attatcagaa gtgaataatt agccattctg tattccgcta atgggcaata tttttaaaga
      421 agaaaaggaa actataaaat attaacagcc tcctagcgat gccgaaaagc cctttgataa
      481 aaaaagaatc atcatcttaa gaaattctta gtcatttatt atgtaaatgc ttataaattc
      541 ggccctataa tctgataaat tattaagggc aaacttatgt gaaagggtga taactatgag
      601 cgataaaata cttattgtgg atgatgaaca tgaaattgcc gatttggttg aattatactt
      661 aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat tggaatgtat
      721 agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca caagcggcct
      781 tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc tgaccgggaa
      841 agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt atataacgaa
      901 gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc gatacaaaaa
      961 attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc ttgtcattaa
     1021 tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc ccaccgagtt
     1081 ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg agctgctatt
     1141 tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca ccgtgcatat
     1201 ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata taaaaacggt
     1261 atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa ctagaacgaa
     1321 aactttacat gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg ttgtatattc
     1381 gttcaatgat ccgagggaaa cttggggatt ggatcttaag tattttggaa aacaaatatg
     1441 acttaaatca cctggacgcg atgaaattat atcaatattc catacggaac aatatagata
     1501 tctttattta tgtggcgatt gtcattagta ttcttattct atgtcgcgtc atgctttcaa
     1561 aattcgcaaa atactttgac gagataaata ccggcattga tgtacttatt cagaacgaag
     1621 ataaacaaat tgagctttct gcggaaatgg atgttatgga acaaaagctc aacacattaa
     1681 aacggactct ggaaaagcga gagcaggatg caaagctggc cgaacaaaga aaaaatgacg
     1741 ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc ggttatttga
     1801 gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat gtgcatatca
     1861 cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag attacacggt
     1921 ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat atgctggtgc
     1981 agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg gttattcacg
     2041 cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc tttaacaaca
     2101 ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt accgcgggcc
     2161 tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca aaagataagc
     2221 tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc gatacgggtg
     2281 gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga gggcagattt
     2341 acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg atgccagact
     2401 tggttgataa aaggaggtcc taagagatgt atata
//
LOCUS       DASHFF010000143         2419 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.149, whole genome shotgun sequence.
ACCESSION   DASHFF010000143 DASHFF010000000
VERSION     DASHFF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.149"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..288
                     /locus_tag="U2I75_002849"
     CDS             <1..288
                     /locus_tag="U2I75_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697895.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(801..874)
                     /locus_tag="U2I75_002850"
     tRNA            complement(801..874)
                     /locus_tag="U2I75_002850"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(838..840),aa:Ile,seq:cat)
     gene            complement(1063..2082)
                     /locus_tag="U2I75_002851"
     CDS             complement(1063..2082)
                     /locus_tag="U2I75_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN1697896.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2093..2299)
                     /locus_tag="U2I75_002852"
     CDS             complement(2093..2299)
                     /locus_tag="U2I75_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN1697897.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          739 a          465 c          405 g          810 t
ORIGIN      
        1 gagacacaat tatggtttaa atttgaccct tcgaatagat ttatcaaaga tttttataaa
       61 gtatggaatt cagaagtttt ctttttagca atcgaagata gcttattaat caatctctac
      121 tattctaata agaactattt caaaattcct gctgcgaaaa ctagaatgaa gaaggatgta
      181 tactttttgt ttgatatcgt gactgacgtg ccggacgcac gtagcgatca tcggcgttat
      241 gactatataa agtatacttt cgttgatcca gaaagataca aagattaaag taggctacct
      301 aaaaaggtag cccggaacga attttatcac catacttgtg aaaggagata ttttttaagt
      361 tagtattaag attgtgtaat atgatgatat ctatatttta tagtatcagt gctataaaat
      421 caaaaataag tcactaatta actaccactc caattgtaag ccttttttct cacttttttt
      481 caaaaatatg gtatgccttt taatagcttc aaatataaaa gagtttaaag cgtaacacac
      541 ttatggggaa gtggcttggg gtgcgcttta aactcttctt tattatcatc tcacaattta
      601 acccaaatgt ctttctattt aaaaatcaaa gtaaaacttt tcaaatatac agaaatataa
      661 ctatgtgaaa catcctttca ttaatccata aaaggataca taaaaaagcc actcatttga
      721 gtggcaatga agaaaagctt tagcttgtat aatactcttc aaaaaattct aacacagaac
      781 gattcaaatg gctacgttaa tgtaccctgt aggactcgaa cctacgaccg gacggttatg
      841 agccgtctgc tctgaccaac tgagctaagg gtactggttg ttgccacata aagccataaa
      901 caatcaacca gtagaatgtg tggcaacaaa cctgttatcg catagcttgg agtgtgacta
      961 ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaatga tttttatagt
     1021 tgtcaatata gttatgtaat gctcctcaac gaggagctat ttttatcgtt taggaatatt
     1081 taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc
     1141 attcgttgcc cgcatcatga catagacttt cttattaggg aagttacgca tgttaaaaga
     1201 tacatgatag ccaacatttc cagaagtatt ataagcttga tttacatctg atctataaat
     1261 tccatcagct cttactcgag ctaattcttt cccagtattg taatccataa tgaagatata
     1321 ctcgtattta tagttagcaa tgtgccatcc agctacatgc aaatttgcat tttcgatttc
     1381 tccaaactga tcaatgtggg cgtaattcgt tccatctgtc agtgtaggat tagctgcacc
     1441 agctcgtgtt ggatcaatga caggcttgtt ttcagaagtt gttggatttt catcggtaaa
     1501 tccatgagct aaatcatatg ctaatttttc tttacttacg cccatttcag aaagataacc
     1561 gtaaggatct gtatgatcgc cccagatgtt ttgtgttacc cataaatgcg atttgattcc
     1621 cggttggtta taaggagtgt ccaacgttaa tggaatacca tatttcattg ctgaatctct
     1681 agccaattca acgtatgcct tgtagttttt ctcaaacgtt gctttatcat gtgtgtgttg
     1741 taactcaatc tgcacaggac tgttggcatt agcatacgaa ccagcaccgt actgtacata
     1801 accaggttga ccgacttgat aaacaattcc gccgtctccc acaatataag cagtataagc
     1861 gctagtccat gaacgttgca tatactgcgc ttcattgcgt cctgttgctg tttcattagc
     1921 cgtttcatgc agtaaaatat actgattatt tgctacttgt gagctacctt catttgcgcc
     1981 caaattaaat tcattgttga tagtataggc aaatccgtta attggcaata aaaaaagagc
     2041 cgttaacagg ctcatcgcag taatagtaat tttctttttc atatctatcc tcctatttat
     2101 ctgatgaaat tttcttatac gttcgatttg atacacctaa aatactccct agaaatgctc
     2161 caaaacctgt aataatcatc accgttatat ctgtgtaccg ccagttaaca ccttttccga
     2221 caagaccgac aaaagtcgct aaagcgggga taactactat agcgatccat ttcaaaatat
     2281 caaaggttct attgttcata ctatccacct cccttcattg gttgggctac tttctttttc
     2341 aatcgcttgt tttcttctat caatatttct ctttctgaaa gcaaggcatc aatttgcccg
     2401 tccagttgtt caattttat
//
LOCUS       DASHFF010000144         2395 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.150, whole genome shotgun sequence.
ACCESSION   DASHFF010000144 DASHFF010000000
VERSION     DASHFF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.150"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            316..570
                     /locus_tag="U2I75_002853"
     CDS             316..570
                     /locus_tag="U2I75_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1697898.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="U2I75_002854"
     CDS             571..888
                     /locus_tag="U2I75_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1697899.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="U2I75_002855"
     CDS             1447..2382
                     /locus_tag="U2I75_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697900.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          338 c          453 g          743 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgatg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DASHFF010000145         2246 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.152, whole genome shotgun sequence.
ACCESSION   DASHFF010000145 DASHFF010000000
VERSION     DASHFF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.152"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..581)
                     /locus_tag="U2I75_002856"
     CDS             complement(<1..581)
                     /locus_tag="U2I75_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697901.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLG"
     gene            complement(591..1328)
                     /locus_tag="U2I75_002857"
     CDS             complement(591..1328)
                     /locus_tag="U2I75_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1697902.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            complement(1379..>2246)
                     /locus_tag="U2I75_002858"
     CDS             complement(1379..>2246)
                     /locus_tag="U2I75_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1697903.1"
                     /translation="NSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIW
                     NSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDG
                     KNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNIS
                     IPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLP
                     LKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSI
                     TKIQNDNASAWR"
BASE COUNT          671 a          451 c          354 g          770 t
ORIGIN      
        1 cctagacttt tttcatgacg taatgtgtta ttatctttta gaaactcacc atgaaatcga
       61 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
      121 acattagcaa gcgtatctac taatgacgag aaagaaaact gcgaagggat agctggtttc
      181 gctaaaactt ttgcgttttg ccatgcctga gtagcagtga tcttttctgt tccgctgtca
      241 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
      301 gtattgccta ttacagaagt ttttgcataa taaatccgta gacgctgttt tgctcgattt
      361 tcatctacat aacactgaat aatacgtccg tctacaatca aatctgcatt agttccgttt
      421 attgaataag taccctgaaa tatctcggct ccgtttagtt tattgctaac agtagctgtt
      481 aaccagtctg acaaagcgcc taaaccttgc gtatcatata aatgttcagc taaattattc
      541 gcgtcatttt tatcgtaaat agttattaaa ttatcgatca tctatttcac ctaccttaac
      601 ccgttacgat aaatttgtat tttgctcaaa ccagtgcaat taaaatgatt gatatccact
      661 tgcaatgtcg gatattgcat ggtcttcatt ttgttggacc gatctaaaat atctccgtcc
      721 gattgctctt cgtagcaaag catcaaatca ctatcaatga ctacatcagt tcctgctact
      781 aagccttcga aactaaacac ataatcattt aagatgaact ggcatgaagt agctgaagga
      841 gtgatgataa tctttggaaa actttcttct aaactattat tcagcaagtt aaatgactgt
      901 ggtttatcta cggttatagg tacatcttct tgaactcttg cgaatggttt cgcagtaata
      961 tttacatcga actctcccca ttcaacaata tcgttttctg catccccaat atcgatagtc
     1021 tggataacgt aatagacgtt gggatcgtca gagaattcta atttctttgc atagtttaac
     1081 caatgacgca tgatataaaa cgattgcttg aacgcttgat ggtcttccac atcctctaaa
     1141 tagttatagt gcaatgtaaa cgacatatct tcaaacgagt aatcttgtac taagccacct
     1201 aacctaccta aaacagaagt ttcaactctc tgtctttttg gagaaggtat ggttggtctt
     1261 tcagctaaag ccaatttatg caaataatca ggaaatccat cgattataga atgtatacaa
     1321 tcagtcattt tttcacatcc tttttaatac taaaaaaaca ggagaaatac tctcctgttt
     1381 aacgccatgc cgaagcatta tcattttgaa tttttgtaat gctatcaatg atttgttgag
     1441 ttgtttgctt catagtaacc tcatctgcgt taccatcaat tgtgaaattg aattcgtagt
     1501 tattcacagg ttgaatcgtt tgtgccctag atgaaactga tgtgctactc aagatacgat
     1561 caccaatttc ttgcaaaaca gatcttttca aaggtaaaac tgcttcaggt cctgcttcac
     1621 cgacaccgtt cattccacct agtaaagttg gtttagtaaa gatacctcct ttagcatgcc
     1681 attttacacg caaatggggg atttgacctt ttagcgggtt aaagctgcct tccatgataa
     1741 attccggtaa cggaatatgt ggtatagaaa tattcaaatt atcaaaaatg ccactgattt
     1801 tgtctctaat ccaatcaatt ggagcgctaa cagtcttttt gatgccttcc catatgttag
     1861 caattgtact tttaacatta ttgaatatgt cggaaacaat acctgttaga ttggaccaac
     1921 cgcttgaaat tgcatttttt ccatcgttta ctttagagct aatagtgctt gtaattccat
     1981 tccaaagatt caaagcagta tttttgatac cgttccaaat tccgctaatc cacgaagata
     2041 tactattcca aacactttga atggcacttt tagctgcgtt tatagcattg cttatactac
     2101 tagtcacact attccagata tttgatgctg tagagctgat tgaattccaa attccaccta
     2161 accaactaga tacagttgac caaatatttt gaattactgt agcagctgct tgtaccaagc
     2221 tagtgattgt attcttgata ctgttc
//
LOCUS       DASHFF010000146         2176 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.154, whole genome shotgun sequence.
ACCESSION   DASHFF010000146 DASHFF010000000
VERSION     DASHFF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.154"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            14..679
                     /locus_tag="U2I75_002859"
     CDS             14..679
                     /locus_tag="U2I75_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697904.1"
                     /translation="MAYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQN
                     LTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVG
                     FILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEV
                     TVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGK
                     VVI"
     gene            723..992
                     /locus_tag="U2I75_002860"
     CDS             723..992
                     /locus_tag="U2I75_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697905.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1224..1520
                     /locus_tag="U2I75_002861"
     CDS             1224..1520
                     /locus_tag="U2I75_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697906.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1522..1944
                     /locus_tag="U2I75_002862"
     CDS             1522..1944
                     /locus_tag="U2I75_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697907.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1963..>2176
                     /locus_tag="U2I75_002863"
     CDS             1963..>2176
                     /locus_tag="U2I75_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697908.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKT"
BASE COUNT          820 a          296 c          422 g          638 t
ORIGIN      
        1 ggtggtctat ggggtggcct attacttgtt aagtgagcca aaacagatcg cggcgtcaaa
       61 tattttcacg gcagtaatga cattaatgtg tgtcatgtgc ttttctattt ttagttactt
      121 actcgtccaa tttatcaggc aaaatttaac aatgagtaaa cttttcaaaa gtttattgga
      181 cttacttatc ttaggcagta tgttaacgac tctaacagat cggttcgaac aatctgaaaa
      241 aacggtagag gggataaaga acttcttaac gacgattgat caatttataa atggatatat
      301 gtatttgttt gaagtaggct ttattctcac tttgtttggt tgtgtattta taaaactcat
      361 taaaagatca atcgaaaaag aaatggttat tgatctaatt ccgacgctga gcggctatga
      421 gtattacgtt gattttggaa agtatgagga gttgatccat acggatatcg ttcacacgag
      481 tatccatgaa gtaaccgtta agggcatcag atctacttta aaagatggac aggagacgaa
      541 aacaatcgac cgaagaaaag aggtctacaa tttttataaa gttgaagcaa gtctacgata
      601 tggcattggg aaattgaggt tagatgggag taatattcaa caaaacaaac catatgtttt
      661 aggaaaagtg gtgatttaga acttcctatg aaagatgaat aaataaggag aatcttttga
      721 aaatgattct tctttacaga attgtgctta tagttggcac ggagttaaaa atcttaaaga
      781 aggagcagtt attcaaagtg aaagaacatc ataatcagtt actatgtatt aaaacgggta
      841 cgatgaacat tcaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
      901 cgattcgaga gcaacataag caagaaaaat taatggctac ttatagccaa ttgattgatg
      961 agtgggaaca attcaaggaa tctaaaacat aaaattccat ttaaacacct tattttttaa
     1021 agagcataag gaaacgtgac cccactttta aaaaaagtgg ttggataggc tgtgcctatc
     1081 acgtttctga aaaaaataaa tgaccccaaa ataccaatat ataggatagt acaaggatag
     1141 caaaggatag taaaaggata gaggtaggat agcgaaaagg ataggatcct tttcactatc
     1201 ctatattata ttggaggata tttatgaatg ttaaaattag agatttagat ccaaaattca
     1261 tttctgaaat tgatcgaaga tgcgttgaac taagtaatcg tacaggaaat aaatggagtc
     1321 gtaatgatta tttaaagcta ctcgttgaaa atgattttga tcgtcctttg atggaataca
     1381 aacaagaaaa gtttgaccag ttattagaaa agttttcaga aattcaaagt tataacacaa
     1441 agatattaga agaatatgtt agccaaaata atcgtattat agaaatcttg attgagcaaa
     1501 atagaggaag tgaactttga tatggcaaag gttatagaca aacacattta ctttcaccaa
     1561 acacatatcg atgtattgga tgaaactatt tctactcatg cagaggttaa aaactatagt
     1621 gaagctgttc gctttttatg tatgaactat gatccagaag tggagaaaaa agtaaattcc
     1681 aatcaagcaa agttgaatgg aatgagtaaa gaaatttcta ttttaaccga aatggtttca
     1741 gaatttgcgg atcttcattt gaaagataca ggaatattaa ttggtaaaaa ttcaactgtt
     1801 tatcaagaag caaaagaacg agtggaagga aaaattaaat ccaatcaaac aaaaaaatat
     1861 tcccctcagt tgaagaaacc tgtgaatgaa tctgttcatg ccagagtaga tccatttgat
     1921 ccaacgaaga ttctaaaaag ataaaaatga aggaggataa taatgactca atcgccttca
     1981 gttattctca cttgtcaatt tactttgccg aataaaaagt cattttctac ttatattgac
     2041 tatattacaa gggaacaggc tcttgaagaa aaggaacgac gtacgcccga agaagaacag
     2101 gaacttcaac gaatcaagaa tgctttagag gagttttata ttcctgaagg agaaactttt
     2161 tcggagagta agacgg
//
LOCUS       DASHFF010000147         2056 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.155, whole genome shotgun sequence.
ACCESSION   DASHFF010000147 DASHFF010000000
VERSION     DASHFF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.155"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            493..714
                     /locus_tag="U2I75_002864"
     CDS             493..714
                     /locus_tag="U2I75_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697909.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="U2I75_002865"
     CDS             715..972
                     /locus_tag="U2I75_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697910.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="U2I75_002866"
     CDS             complement(1108..1710)
                     /locus_tag="U2I75_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN1697911.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="U2I75_002867"
     CDS             complement(1777..1932)
                     /locus_tag="U2I75_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1697912.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          464 c          313 g          601 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatca ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DASHFF010000148         2003 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.158, whole genome shotgun sequence.
ACCESSION   DASHFF010000148 DASHFF010000000
VERSION     DASHFF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.158"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..378
                     /locus_tag="U2I75_002868"
     CDS             <1..378
                     /locus_tag="U2I75_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN1697913.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="U2I75_002869"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="U2I75_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN1697914.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="U2I75_002870"
     CDS             1859..>2003
                     /locus_tag="U2I75_002870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697915.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DASHFF010000149         1991 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.159, whole genome shotgun sequence.
ACCESSION   DASHFF010000149 DASHFF010000000
VERSION     DASHFF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.159"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="U2I75_002871"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="U2I75_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN1697916.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="U2I75_002872"
     CDS             852..1040
                     /locus_tag="U2I75_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN1697917.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="U2I75_002873"
     CDS             1053..1610
                     /locus_tag="U2I75_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1697918.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="U2I75_002874"
     CDS             1642..1884
                     /locus_tag="U2I75_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1697919.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          553 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc c
//
LOCUS       DASHFF010000150         1933 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.160, whole genome shotgun sequence.
ACCESSION   DASHFF010000150 DASHFF010000000
VERSION     DASHFF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.160"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            434..601
                     /locus_tag="U2I75_002875"
     CDS             434..601
                     /locus_tag="U2I75_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697920.1"
                     /translation="MVYLNKKQTCEFLGIDNNTLDKWIRIYNFPVIKIDGIIRFDVED
                     IKGFMDTFKKI"
     gene            675..1901
                     /locus_tag="U2I75_002876"
     CDS             675..1901
                     /locus_tag="U2I75_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1697921.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
BASE COUNT          721 a          291 c          303 g          618 t
ORIGIN      
        1 tattttaaat ttttacatac ctgtttatga aaaactaaat aatctgtcaa aaaaatatga
       61 ttgttcattt ctaaatatgt tttccaattt taaaatcttt tttagcattt ttgtttcctt
      121 ttcatatggt ttttcgttgt taatagtgac taggatgata ttcctatttt gaccattgac
      181 aattaaatat aatttttgaa taacgttccc tgctattcga gctaagaatt caagtacgcc
      241 atgacctatt tagcgataac tacttagaga tttctttttg ttttgggaag acaaaatgcc
      301 atgacaatag cagtagataa tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg
      361 aaattcctat gtgcttaccc aagtcaattc aaaaatttta gttcatttat aaggggatga
      421 ttttattaaa tcaatagtct atctaaacaa aaaacaaacc tgtgagtttt taggaattga
      481 taacaatact ttagataaat ggattcgtat atataatttt cccgtaataa aaatagacgg
      541 tataattcgc tttgatgtag aagatatcaa aggctttatg gatacgttca aaaagatttg
      601 aattttactt ttgactgcat atactaaaag tagcgaaata tactgcctta cttttttatt
      661 aaggagtgag gaagttgcct acgaagcttt caaatggaaa atataaaact aatttgagat
      721 atccaaaaaa atttaaagaa attacaggta tcagttcaga aaaattccaa aaaacatttc
      781 caactaggca attggctatt aaagccgaaa atgaaatgaa aaagaaaatc gcaaaagtct
      841 taagagagga gaatgcaaat tcattagagc tcaaaggtca aataaaattc aaggagtttt
      901 ataaatctaa atggcttcct agatatgagt tagggcaaac tactagatcc cataaagctc
      961 caagcgatat aaccatcatt aatacaaaga atttatttcg attgcatatt ctgcctatat
     1021 ttggtgagta tgccatgagc tatcttaatg caaataccga aataatatca gatgaactca
     1081 caaaaaagtc gaaggaatat gcaaacataa aaatcattaa atgctatgtt cgttccatgt
     1141 ttgatattgc agaggttcta aattatattg aatttaatcg tacaacaaaa ataattcaaa
     1201 gtattactgc acctaagaaa gttgctctgg aagaaaaaag aatacgagaa ggaaaacaag
     1261 ctttatcttc gaaagaactc actgattggt tagacgctgt aagagatgat ttcaatgatc
     1321 aattactttc ctttcacgat tacactcttt ttatgcttac tttatatctt ggggatagaa
     1381 agagtgagac ctatgctttg caatggaaat acattgattt tgaaaaccaa acagtccgtc
     1441 ttaaatatgc cttggataaa cacacaaaaa agaaatttac aaaaggtcga aaagatacta
     1501 ttatccaagt tccggaaatt gtaatgacac tattgcaaga atggaaatca gtgcaagctg
     1561 aacaactatt gaagttgaaa attaaacaga cacctgacca atatcttttt agttactcca
     1621 aaccttctgg tgaagtaaat tgtccagtcc atactgatta cttaaattat cgaattaatt
     1681 ctataaaaaa aagacatccc catttggttc atttaactcc tcataaactt cgacatactt
     1741 acgcaacttt agctagacaa ggtggggctg atatgaatca aatttctaac gctttgacac
     1801 attcaggtat ttcaactaca aaaatttatg tgaatacacc tgatttagtt gataaagctg
     1861 tgttcgaagc gtttcagcga ggattacaaa aatctaatta atgtgttcta gaatgtgttc
     1921 taaatataaa aaa
//
LOCUS       DASHFF010000151         1686 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.163, whole genome shotgun sequence.
ACCESSION   DASHFF010000151 DASHFF010000000
VERSION     DASHFF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.163"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            109..1278
                     /locus_tag="U2I75_002877"
     CDS             109..1278
                     /locus_tag="U2I75_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1697922.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          600 a          274 c          281 g          531 t
ORIGIN      
        1 aaaagatagc gtaaaatatt ttgtgtaaat agaaatttag ggtagaaaaa aaagtccatt
       61 ctgtaaaatt aaagttacca cacagaaatt ttataggagg actttctcat gactaaattt
      121 actatagaaa ttatgcaaac actactcaat aaaggggatt tagatgaatt atttcaggat
      181 catttagagc gagcgattaa ttcacttcta caagcagagc taactgcttt tttagattac
      241 gaaaaatatg atcgaaatgg attcaattca ggaaattccc gtaacgggaa ttattcacgt
      301 acatttaaaa cagaatatgg cgagttgaat ttaactattc ctagagatcg gaatggcgaa
      361 ttttctcaac aaacactacc agcatataag cgaaccaatg attcattaga aacgaccatt
      421 atccaattat ttcaaaaagg cattactatg gctgaaatct ccaaattaat tgaaaaaatg
      481 tatggtcacc actatacgcc acaaacaatc tccaacatga gtaaattggt ggctgaagat
      541 gttttagctt ttaaagaaag aacgttagaa gccaattatt ccgttatatt tatggatgcg
      601 acgcatatcc ctgtgaagcg acagactgtt tcgaaggaag cagtgtatat tacgattggt
      661 attcgtttgg atggaaccaa agaagttctt gggtttacta ttgccccaac tgagtctgct
      721 tatatttgga aagaagttct tcaagatctt agaaaacgtg ggttagaaga agttttatta
      781 gtagtgacag acggattaag ctgtattgaa gaaagtatcc atagtgtgta tccgaatgcc
      841 caatttcaac aatgttgtgt gcatgtatct agaaatatcg ctcataaagt tcgtgttcga
      901 ggtcgaaaag aaatttgtga ggatttcaaa ttggtttacc aagcgaattc aaaagaagag
      961 gcattggatc acatcgactt tatgactagg aaatggaaaa agcagtatcc aagagtcgtc
     1021 aatttactct tgaatcctgc cctattaacc ttttataatt tccctcacgc catcagacga
     1081 acaatttatt cgacgaacct gattgaaggc tttaataagc agctaaaacg atatactcga
     1141 agaaaagaac aattccctaa tgaagaatct ctagagagat tcttcatttc tcaatttaat
     1201 caatataacc aaaaattttt aggtagaatt cacaagggat ttaaagaaat tcaggataca
     1261 ttagagtcga tgatttaact gtaattaaca gaatggattt tccatttaca tataattctt
     1321 gacgctacca taatctaaat aaccatcata ttcatctact acttatataa ttgagtttta
     1381 ttaaactttt taagtttcta ttctatcaaa tatttaaaca atctctgcat ccgaaaagac
     1441 tacctcgtat agttttacta acaatatttt tattagcgtg ttcaaatcct gatttcttgt
     1501 atcaattatt ttctgttcac tttcaataag aaaaaagtct ttacttccat ggataataat
     1561 aagggttctg ttgcaaagtt ttaaataaag aataaaatcc cttacggtat ctatgattta
     1621 agctgggatt cccaataata ccttgatttc agtacagacc gaaaacccga agagagtgcc
     1681 ttcttt
//
LOCUS       DASHFF010000152         1643 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.164, whole genome shotgun sequence.
ACCESSION   DASHFF010000152 DASHFF010000000
VERSION     DASHFF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.164"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..779
                     /locus_tag="U2I75_002878"
     CDS             <1..779
                     /locus_tag="U2I75_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1697923.1"
                     /translation="DGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALFEKNY
                     RAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVS
                     KEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANL
                     HVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMR
                     NFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            969..1042
                     /locus_tag="U2I75_002879"
     tRNA            969..1042
                     /locus_tag="U2I75_002879"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1003..1005,aa:Ile,seq:cat)
BASE COUNT          533 a          281 c          315 g          514 t
ORIGIN      
        1 gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg ggagctggta
       61 acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat aaagcattgt
      121 ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa aaatatggaa
      181 ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt tctcatttgt
      241 gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta tcgcaaatgg
      301 gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat gaaaatccaa
      361 caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct aatcctacgc
      421 tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa aacgcaaact
      481 tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc attatggact
      541 acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga ccagatgtaa
      601 accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat atgcgtaatt
      661 ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag ggaaacacta
      721 aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct aaacgataaa
      781 aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa attattcgat
      841 aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca ctccaagcta
      901 tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc tttatgtggc
      961 aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg tccggtcgta
     1021 ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg ttagaatttt
     1081 tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg agtggctttt
     1141 ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact tctgtatatt
     1201 tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg tgagataata
     1261 ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt acgctttaaa
     1321 ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa gtgagaaaaa
     1381 aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat agcactgata
     1441 ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa aaatatctcc
     1501 tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc ctactttaat
     1561 ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc cgatgatcgc
     1621 tacgtgcgtc cggcacgtca gtc
//
LOCUS       DASHFF010000153         1570 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.165, whole genome shotgun sequence.
ACCESSION   DASHFF010000153 DASHFF010000000
VERSION     DASHFF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.165"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            378..716
                     /locus_tag="U2I75_002880"
     CDS             378..716
                     /locus_tag="U2I75_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1697924.1"
                     /translation="MNGIEDENLRRLINSLVIELYKYQAESERKKIKERQAQGIEIAK
                     KKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVD
                     QRKNNEKRDS"
     gene            808..1513
                     /locus_tag="U2I75_002881"
                     /pseudo
     CDS             808..1513
                     /locus_tag="U2I75_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320596.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT          594 a          252 c          288 g          436 t
ORIGIN      
        1 ttcaatttgg cgttgctcat tttgatcaat cgacgaaact cgaatataag caattttcaa
       61 aatttttacc tacttcctca aactatttaa aatacactca aaatcaacac taattttttt
      121 atacactata aagctgtcat ttttctagat aaatggcata aattttcagc gctaagtgac
      181 aaaagcaact aatttttcat gatagatttt taaatatctc aatctatctc caaaattagt
      241 tgcttttgta gctatagttg actatgaata tgacaaataa ttagaaaaaa ctgtgccaat
      301 taaatatcaa aatgacaaat tgatgaatca aattcaaatt aaggggggca accctggaag
      361 tcttaaattt accctcaatg aatggtattg aagatgaaaa tttaaggcgt ttgattaata
      421 gccttgtcat tgaattgtac aagtatcaag cagaatcaga acgaaaaaaa attaaggaac
      481 gtcaggcaca aggaatcgaa attgctaaga aaaaaggcaa attcaaaggt cggcaactga
      541 aatttaaaaa aaatgatcca cgtttaaagc atgctttcga tttgtttttg aatggtttat
      601 ccgataaaga agttgaagaa caaactggga tcaatcgccg aacgtttaga aggtatcgag
      661 caagatataa cgtgacagta gatcaaagaa aaaacaatga aaagagggat agttaatgag
      721 tacggttatt ttagctgaaa aacaagccag gcattagcct acgcaagtgc tttaaaacaa
      781 agcaccaaaa aagacggtta ttttgagatc aaagacccac tatttacaga tgaaacgttt
      841 atcacctttg attttgggca tttagtggaa ttagcagaac caggtcatta tgacgaaaag
      901 tggcaaaatt ggaaacttga atctttgccg atttttcctg atcgatacga ttttgaagtt
      961 gcaaaagata agggaaagca gtttaaaatt gttgcagaac ttctcaaaaa ggcaaataca
     1021 attattgttg caacagatag cgacagagaa ggtgaaaata tcgcctggtc gattatccat
     1081 aaagcaaatg ccttttcaaa agataaaaca tttaaaagac tatggatcaa tagcttagaa
     1141 aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag gaatgaatta ctatcccttt
     1201 tatcaagaag cgcaaacacg ccaaattgcc gattggttga tcggcatgaa cgcaagccct
     1261 ttgtatacgt taaatttaca acagaagggc gtacaaggta cattttcact aggacgtgtt
     1321 caaacgccca ccttatacct tatttttcag cgccaggaag ctagtataaa agtaaaacaa
     1381 gggtcattta agggcgttct aagccccaca cagcgtttta aaacccaaga ggagcttttt
     1441 tgcttttgtt tcttctaaac aagctaaaat aggcaatcaa gaggggataa ttgctgatgt
     1501 tcaaaccaaa gagatggata ggctacaatt aactagacag aaaaattaag gtgtgtagac
     1561 tagaagaaaa
//
LOCUS       DASHFF010000154         1522 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.166, whole genome shotgun sequence.
ACCESSION   DASHFF010000154 DASHFF010000000
VERSION     DASHFF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.166"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>1522)
                     /locus_tag="U2I75_002882"
     CDS             complement(<1..>1522)
                     /locus_tag="U2I75_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697925.1"
                     /translation="DFSKELNYTKAIMARQGDKGITVTVKPFLNGLQMDTSGGTFTLK
                     GTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQILTTQDIIFF
                     SLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQITDLTGQAKTL
                     QDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVGPLLGNGHTVK
                     DSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTYTLSVTMSVGS
                     DWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGITKDNVTSITNC
                     YVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIADPTVVFPHKTS
                     EYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMKPTEGLANTWE
                     LTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNISEYKYRGTG"
BASE COUNT          424 a          309 c          258 g          531 t
ORIGIN      
        1 ccagtaccac gatatttata ctcactaata ttcggggttc gagtattgcc tttttctagt
       61 ttgagccact caatttgaac agctcctttt gtagctgacg ggtattggta tatttccaga
      121 taattagtta ccccactatc tatatgttgc ttagttacag taaaagttaa ctcccatgta
      181 ttagctaacc cctcagtagg cttcatgtct cctactttaa cgcggccagc agcaatatac
      241 acggcaaacg attgaactgt tagtttagtt gctttcattg atatggtata tgtttggtca
      301 gcgatatagt tttctgtatt gcgtctacca taaaccatat attcactagt tttgtgtgga
      361 aagacaactg tagggtcagc aatattctcg cctaaaggaa ctttacctaa ccagtaaggg
      421 ccatctagta agttagggta gtgtgctttt aacttaacgt catagctgac actaaccgtt
      481 cctttatatt cactattaag tgagaatatt tcaacataac agtttgttat gctagttaca
      541 ttatcttttg ttataccagt aaatgtatga gtgaccattt tatcgcgttc tacatttgct
      601 ggtattaatg cattaatggg cagttctata ccaccttgtt catttaaata tctaagccta
      661 acccctatag tattggtctc tccagtccaa tctgaaccga cactcatagt aacgcttaat
      721 gtgtatgtcg tagatggtcg taaagtggtt ataagatacc tattattagc ttccgctgta
      781 cacggaatac ctaagccaag cgataatatg tcgccggaat tagctgttct agttttagta
      841 tgagttataa cccttttaac actatcttta actgtatgtc cattacctaa caaaggtcca
      901 acccaaggtt ctgtaatata tggcattaaa ttcgggttct ccgaataatc atagtccccg
      961 aagtcgatgc tgttactgta catcacttgt aagttaccta acttagaaat ttcttctttc
     1021 agagcatcta acttgtcttg tagcgtttta gcttgaccag ttaaatcagt aatctgttga
     1081 tttaagctat ccactctacc tttgatttca gccataaaag catcaaaagt ttcgttgtac
     1141 tttcgaatca actcttctaa ttgcgaaaca tattcatcgg cttggccttg cgaaatgtca
     1201 gacactccta gtgagaaaaa aatgatatct tgcgttgtta aaatttgatt gtcttttcta
     1261 tattctacgt agcagtgttt ataatatcct gcttcactca taaatgtgcc atcaagagaa
     1321 aacgtgactt cttcactagt tacactagtt gcaacattat ctacgtaacg gttagatggt
     1381 gttgttcctt ttaaagtaaa tgttccgcca ctcgtatcca tctgcaagcc atttagaaac
     1441 ggtttaaccg tcaccgtaat ccctttatca ccttgacgag ccataatagc tttggtgtag
     1501 tttaattctt tgctgaaatc ta
//
LOCUS       DASHFF010000155         1502 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.167, whole genome shotgun sequence.
ACCESSION   DASHFF010000155 DASHFF010000000
VERSION     DASHFF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.167"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..182)
                     /locus_tag="U2I75_002883"
     CDS             complement(<1..182)
                     /locus_tag="U2I75_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1697926.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            577..1269
                     /locus_tag="U2I75_002884"
     CDS             577..1269
                     /locus_tag="U2I75_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1697927.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          615 a          210 c          253 g          424 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccgcgtt
      301 ttttgtaata aatacagtaa tctatgaaat gaaaagggtt acgtttaata aaacttgctt
      361 ttttgttttt gttcaccata aatatttatt taagaaagta ttaacctgca tttttctcta
      421 taaaaaagat tttttaaata gataaaaaag gaggattaaa tgagtatttg atagtattta
      481 tattaaaatt acatatgtaa tttaaaaacg cttgacattg tgtttgagat cacttacttt
      541 tagcatgtaa aagtattgga ttaaggagct atgcaaatga aaaagaaatt tattactggt
      601 gggattgtcg ttgcatttct atgtctaatt ggctacacag gcacgcggta cgcaaatgaa
      661 attattttgt ggggtggaga gacagatatt gatacaatca atgagaatct gacacagctt
      721 gatcatacat tgacagataa agagcaagcg attacagatc ttaccgctcg actagcacaa
      781 aaagaacaga cattgcaaga ttcaaaggca gatgtccaag aataccaaac aaaagtaaat
      841 gaattagaaa accaaaaaaa tcaatcggca gcagaaaaac aaaacttaga aagtcaatta
      901 gtcagcaagg atgcacaact acaaagcaaa caaaatgaaa tcaatgtcaa actagatgag
      961 atcaaccaaa aaaatgatga aatcaatcat ctgaatgcag attggacagc aagactacaa
     1021 gaagcacaag caaaactttc agaattacaa aatactgtaa atggtttgaa tcaacaaatc
     1081 gcaacactga cacaagaaaa aacaaatgta gaaactcagt taacagatac acaaaagaaa
     1141 ttagacgaag caactgctga aaactcaagc ttgaaacaat atattcaaaa gttagaaaaa
     1201 gcaaaagatg aagtggaaga tacagctgat aaatctcagc aaattgttga agaacatttg
     1261 cgtaaataat ctttcttaac aagtaaacct aaattagtaa agaagcaaca gtaatctata
     1321 ctaagtagtt cagttttgga gtacaaaatg acaaaatgta cttcaaaatg gactactttt
     1381 ttcattttaa cttacatacc aaattacata caaaatgatt tataaaatgc aattgaaaat
     1441 gataattgaa tgaaaatttg tgattttgtg gtattatgat aatataactt atagaaaaaa
     1501 tt
//
LOCUS       DASHFF010000156         1466 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.168, whole genome shotgun sequence.
ACCESSION   DASHFF010000156 DASHFF010000000
VERSION     DASHFF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.168"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(1014..1304)
                     /locus_tag="U2I75_002885"
     CDS             complement(1014..1304)
                     /locus_tag="U2I75_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697928.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          469 a          288 c          205 g          504 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tataga
//
LOCUS       DASHFF010000157         1456 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.169, whole genome shotgun sequence.
ACCESSION   DASHFF010000157 DASHFF010000000
VERSION     DASHFF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.169"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            108..1040
                     /locus_tag="U2I75_002886"
     CDS             108..1040
                     /locus_tag="U2I75_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEN1697929.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="U2I75_002887"
     CDS             1112..>1456
                     /locus_tag="U2I75_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697930.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DASHFF010000158         1424 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.170, whole genome shotgun sequence.
ACCESSION   DASHFF010000158 DASHFF010000000
VERSION     DASHFF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.170"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(150..1337)
                     /locus_tag="U2I75_002888"
     CDS             complement(150..1337)
                     /locus_tag="U2I75_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN1697931.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DASHFF010000159         1421 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.171, whole genome shotgun sequence.
ACCESSION   DASHFF010000159 DASHFF010000000
VERSION     DASHFF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.171"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1407
                     /locus_tag="U2I75_002889"
     rRNA            <1..1407
                     /locus_tag="U2I75_002889"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          388 a          305 c          429 g          299 t
ORIGIN      
        1 ccaagcagta agtctgaaga ggagtcaaat gcttcttttc ttaaggacaa gctgtgatgg
       61 ggagggaaat aatagtaccg aagttcctga tgtcacactg ccgagaaaag cttctagtga
      121 gaaaacagcg gcccgtaccg caaaccgaca caggtagtcg aggagagaat cctaaggtga
      181 gcgagagaac tctcgttaag gaactcggca aaatgacccc gtaacttcgg gagaaggggt
      241 gctgatcata cgatcagccg cagtgaatag gcccaagcga ctgtttatca aaaacacagg
      301 tctctgcaaa atcgtaagat gaagtatagg ggctgacgcc tgcccggtgc tggaaggtta
      361 agaggagtgc ttagcgcaag cgaaggtacg aattgaagcc ccagtaaacg gcggccgtaa
      421 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg
      481 cgtaacgatt tgggcactgt ctcaacgaga gactcggtga aattttagta cctgtgaaga
      541 tgcaggttac ccgcgacagg acggaaagac cccatggagc tttactgtag tttgatattg
      601 agtgtctgta ccgcatgtac aggataggta ggagccgtag aaatcggaac gctagtttcg
      661 atggaggcgc tggtgggata ctacccctgc gttatggcca ctctaacccg caccactaat
      721 cgtggtggga gacagtgtca gatgggcagt ttgactgggg cggtcgcctc ctaaaaggta
      781 acggaggcgc ccaaaggttc cctcagaatg gttggaaatc attcgaagag tgtaaaggca
      841 gaagggagct tgactgcgag accaacaagt cgagcaggga cgaaagtcgg gcttagtgat
      901 ccggtggttc cgcatggaag ggccatcgct caacggataa aagctaccct ggggataaca
      961 ggcttatctc ccccaagagt ccacatcgac ggggaggttt ggcacctcga tgtcggctcg
     1021 tcgcatcctg gggctgtagt cggtcccaag ggttgggctg ttcgcccatt aaagcggcac
     1081 gcgagctggg ttcagaacgt cgtgagacag ttcggtccct atccgtcgcg ggcgttggaa
     1141 atttgagagg agctgtcctt agtacgagag gaccgggatg gacttaccgc tggtgtacca
     1201 gttgttctgc caagggcatt gctgggtagc tatgtaggga agggataaac gctgaaagca
     1261 tctaagtgtg aagcccacct caagatgaga tttcccattt ctttaagaaa gtaagatccc
     1321 tgagagatga tcaggtagat aggtcaggag tggaagtaca gtgatgtatg gagcggactg
     1381 atactaatcg atcgaggact taaccaatag gttaaaagaa a
//
LOCUS       DASHFF010000160         1420 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.172, whole genome shotgun sequence.
ACCESSION   DASHFF010000160 DASHFF010000000
VERSION     DASHFF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.172"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="U2I75_002890"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="U2I75_002890"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN1697932.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          495 a          184 c          236 g          505 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatactata
//
LOCUS       DASHFF010000161         1413 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.173, whole genome shotgun sequence.
ACCESSION   DASHFF010000161 DASHFF010000000
VERSION     DASHFF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.173"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            88..1380
                     /locus_tag="U2I75_002891"
     CDS             88..1380
                     /locus_tag="U2I75_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1697933.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          459 a          288 c          256 g          410 t
ORIGIN      
        1 cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga attaatttgg
       61 cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa agaaacatta
      121 gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga aaaatacaaa
      181 gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga atgcatccac
      241 tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt ccggttgtta
      301 atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg cttcaaatgc
      361 aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca ttgtttcatc
      421 agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc aatgacagag
      481 atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa gcgattttat
      541 cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga tgaattcaaa
      601 tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca aacacagcgc
      661 ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt tagtcatttt
      721 tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc tccttatgta
      781 tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt tcacatcgtc
      841 caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca actattaaaa
      901 agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg gaaacttctt
      961 caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg cttcaaagct
     1021 catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc tttgcgtgta
     1081 ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta cgtatcgttt
     1141 gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac cttgagaacg
     1201 ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc aaatggccca
     1261 cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg ctttcgtagc
     1321 ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat tttcaactaa
     1381 aaaagatcaa gaggaactga tccacttgat ctc
//
LOCUS       DASHFF010000162         1407 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.174, whole genome shotgun sequence.
ACCESSION   DASHFF010000162 DASHFF010000000
VERSION     DASHFF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.174"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>1407)
                     /locus_tag="U2I75_002892"
     CDS             complement(<1..>1407)
                     /locus_tag="U2I75_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1697934.1"
                     /translation="IYTKNLTGLDLEIDAQSVLVGVYPFISSSSEGEDEITLPEEVIF
                     TDYVDDYPAGYVSFVDFKDKATDVATLREAAKDWLKTNIDKQKPQVSGSIELVPLRHQ
                     RGYEKFVDLEKVSMGDGVDVYHPQLKVNMSARIVEYTFNVLTNSYDKLVVGNVKTNFL
                     ENTENNVSNLINDAIDQLKNGGEISDLLNDIVDHQTDMITGQNGGYVLLDPKEAPSRI
                     LIMDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAGEIVGITI
                     RGTTLISDGADYRTSIANGKMTWYSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGF
                     MIRNPQGNLVFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGSSLSINGSAGNSWEF
                     KVAGRTMKFTSDGMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEH
                     YGQRLLNAYETPEYYFADY"
BASE COUNT          432 a          308 c          198 g          469 t
ORIGIN      
        1 ataatcagcg aaataatatt ctggagtttc ataagcgttc aatagtcgtt gtccataatg
       61 ttctgttggt acagttgagt ttttagtacc tctaacagta aaatctttat atacttgtac
      121 cgttgattgt tcaaacctag caagtttccc atcttcccat gaaccaaaaa aacaacctgg
      181 taacgttagc ataccatcac tagtaaattt catagtccta ccagctacct taaattccca
      241 tgagttaccc gcactaccat taatgcttaa agaactaccg tcgccagaag ttacataact
      301 agcattgcta tacctgaaat tgggcgcacc aaaagataga aacggtctgt tattaccatt
      361 atcccacgta ctaaaaacca agttaccctg tggatttcta atcatgaaac caccaccagt
      421 tttcatggtg tatgatacaa taccggcatc agcacttaca taatcacgtg cttctagctc
      481 cataatatct ttgttaactt tttttgagta ccaagtcatt ttgccattag caatacttgt
      541 tctataatca gcaccatcac taattaatgt agtacctcta atagtaattc ctactatttc
      601 accagccgta ataaacgagg cattgaatcc gccatctaac gtccatgccg tttcatatgt
      661 tccattaatg ccagttttag aaaaaccaat accagcattg ttgatttgta aaacattcct
      721 tgcggtattc ttatctggtg tgtccataat caaaatacga ctaggcgctt ctttaggatc
      781 taataaaaca taaccaccat tttgaccagt aatcatatca gtttgatgat ctacaatatc
      841 attgagtaaa tcactgattt cgccaccgtt tttcaattga tcaatggcat cattaatcaa
      901 attgctgaca ttattctctg tgttttctaa gaagtttgtt ttgacgtttc ctacaactaa
      961 tttatcgtat gaattggtta gaacattaaa cgtatattcc acaattctcg ctgacatatt
     1021 cacttttaac tgtggatgat acacatctac tccgtcaccc atcgaaactt tttctagatc
     1081 aacaaatttt tcatagcctc tttgatgcct caatggtact aattcaatcg aaccactcac
     1141 ttgtggtttt tgtttatcta tgtttgtttt caaccagtct ttagcagctt cccttaatgt
     1201 ggctacatca gtcgctttgt ctttaaaatc aacaaaagaa acatatccag caggataatc
     1261 atccacgtaa tccgtgaaaa taacttcttc tggtagagtg atctcgtctt ctccttctga
     1321 agagctgcta atgaatggat aaactccaac taaaacactt tgagcatcaa tctctaagtc
     1381 aagaccagtt aagtttttag tataaat
//
LOCUS       DASHFF010000163         1372 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.175, whole genome shotgun sequence.
ACCESSION   DASHFF010000163 DASHFF010000000
VERSION     DASHFF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.175"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1278
                     /locus_tag="U2I75_002893"
     CDS             <1..1278
                     /locus_tag="U2I75_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN1697935.1"
                     /translation="VGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGRTKVRE
                     EIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRM
                     KFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILH
                     GDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHC
                     DGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWK
                     DFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDG
                     RLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEF
                     LFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          448 a          251 c          292 g          381 t
ORIGIN      
        1 gtaggaacga aagcagacaa aatcaaacac ttacctataa cagagaaaga acatctcctt
       61 cctttggaag aacaattttg tgaacattgc ggttcacaga tgaaagatat cggacggaca
      121 aaagtacgag aagaaattcg tttccatcag gctatgttgg attgtctcac ccattatcaa
      181 catacctatt gttgtaaaag ctgcgaaaaa gaaggacttt cttcttttaa aaaagcgatc
      241 gttcctaaac ctttgatttc taatagctta ggttccaata gtttagtggc ggaaacgatc
      301 cggatgaaat ttggtcagaa agttcctgcc tatcggcaag agaactattg gaaacaaaca
      361 catgggttgg atatttcgag agataatatc accaattggc atattaaagc agtgcaaaac
      421 gcgttagacc ctttaggaga acgattaaga gtctatctta atcaagaaga gattctacac
      481 ggggatgaaa caagttatcg tgtgattgaa agtgccaaaa cagataccta ttactggcag
      541 ttttgtacag gaaaagacag tcagcatccc atcgtttatt atcaccacga cgaaagtcgt
      601 gctggagatg ttccgaaaac ttttctcaag gagtttacag ggtatttaca ctgtgacggc
      661 tatagcgggt ataatgccgt ggaaagtgta cgacttgtct attgtttcgc gcatgttcgg
      721 cggaaatttt tcgaagcaat tccaaaagga aagaaaaaca cggatattcc cgcagctcaa
      781 gcagtcaaac aattggataa atggtttgtc ctagaaaaga aatggaaaga cttttctcct
      841 gagaagcgat taagctgtcg gcaacaagaa ctgcgtccat tatttattgc tttttatgag
      901 tggatggcaa cgatagatcc tgttgcaaaa tccaagttgg atgcagccgt tcaatatgcg
      961 tgtaaactac gaagtggttt tgaacctatt tttgaagatg gtcgtttaga actcacaaat
     1021 aatcgtgcgg agcgaaatat caaagagctg gtgataggac ggaaaaattg gttgcattcc
     1081 acaagtcttg aaggagcacg aacttcagga atcattctga gtgtatataa aacagcagaa
     1141 cttaacggat tgaatcctgt aaagtatcta gagtttctat ttgataagat accaaatctt
     1201 cccgtacttt cagcagaaac tttagatcaa ctgttgcctt ggaacaagga tgtacaacaa
     1261 catttttctc gtaactaaaa acgccaaaca ttcccgattc tattctatag gtcgggttat
     1321 ttgacgtaca ggtactgttc tatggggcgc ttacttttct tcttcaagac ca
//
LOCUS       DASHFF010000164         1368 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.176, whole genome shotgun sequence.
ACCESSION   DASHFF010000164 DASHFF010000000
VERSION     DASHFF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.176"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..>1368
                     /locus_tag="U2I75_002894"
     CDS             82..>1368
                     /locus_tag="U2I75_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN1697936.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DASHFF010000165         1363 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.177, whole genome shotgun sequence.
ACCESSION   DASHFF010000165 DASHFF010000000
VERSION     DASHFF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.177"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            35..1336
                     /locus_tag="U2I75_002895"
     CDS             35..1336
                     /locus_tag="U2I75_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1697937.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          445 a          274 c          253 g          391 t
ORIGIN      
        1 gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc atatcaaact
       61 actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga agagcattaa
      121 gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt
      181 gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat
      241 tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac aacgctacca
      301 ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata gaaactgctt
      361 tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac aagcaatgac
      421 gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct
      481 gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa tggatgaatt
      541 tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg gcgatactgg
      601 aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt attttatgca
      661 atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga atgtttctta
      721 tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc gttttcacat
      781 tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga aaagctttga
      841 tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt ctcggcttct
      901 gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca attttggttg
      961 ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg acgaattacg
     1021 aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag aggcggacac
     1081 tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc attctataaa
     1141 aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc cttattcaaa
     1201 tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg cttatggctt
     1261 tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga ttacctatga
     1321 atcaaaaaac atctaaagta gaaaaggaat tctaccttag atg
//
LOCUS       DASHFF010000166         1350 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.178, whole genome shotgun sequence.
ACCESSION   DASHFF010000166 DASHFF010000000
VERSION     DASHFF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.178"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..>1350
                     /locus_tag="U2I75_002896"
     CDS             82..>1350
                     /locus_tag="U2I75_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEN1697938.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFL"
BASE COUNT          456 a          253 c          259 g          382 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg
//
LOCUS       DASHFF010000167         1316 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.179, whole genome shotgun sequence.
ACCESSION   DASHFF010000167 DASHFF010000000
VERSION     DASHFF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.179"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            86..1165
                     /locus_tag="U2I75_002897"
     CDS             86..1165
                     /locus_tag="U2I75_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1697939.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          357 a          353 c          361 g          245 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaacc
//
LOCUS       DASHFF010000168         1313 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.180, whole genome shotgun sequence.
ACCESSION   DASHFF010000168 DASHFF010000000
VERSION     DASHFF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.180"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>1313)
                     /locus_tag="U2I75_002898"
     rRNA            complement(<1..>1313)
                     /locus_tag="U2I75_002898"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          275 a          391 c          260 g          387 t
ORIGIN      
        1 ctccattgct caaacaaaaa caacgggtac aggaatatca acctgttatc catcgcctac
       61 gcctgtcggc ctcggcttag gtcccgacta accctgggcg gacgagcctt ccccaggaaa
      121 ccttagtcat tcggtggaca ggattctcac ctgtctttcg ctactcatac cggcattctc
      181 acttctaagc gctccagcag tcctcacgat ctgccttcaa cgcccttaga acgctctcct
      241 accaatacac ctaaaggtgt actccacagc ttcggtaata tgtttagccc cggtacattt
      301 tcggcgcagg gtcactcgac tagtgagcta ttacgcactc tttaaatggt ggctgcttct
      361 aagccaacat cctagttgtc tgtgcaaccc cacatccttt tccacttaac atatattttg
      421 ggaccttagc tggtggtctg ggctgtttcc ctttcgacta tggatcttat cactcacagt
      481 ctgactcccg gatatgaatg aatggcattc ggagtttatc tgaattcggt aacccgagat
      541 gggcccctag tccaaacagt gctctacctc catcattctc aattccgagg ctagccctaa
      601 agctatttcg gagagaacca gctatctcca agttcgtttg gaatttctcc gctacccaca
      661 cctcatcccc gcacttttca acgtacgtgg gttcggtcct ccagtgcgtt ttaccgcacc
      721 ttcaacctgg acatgggtag atcacatggt ttcgggtcta cgactacata ctcaaacgcc
      781 ctattcagac tcgctttcgc tgcggctccg tctcttcaac ttaacctcgc atgcaatcgt
      841 aactcgccgg ttcattctac aaaaggcacg ccatcaccca ttaacgggct ttgacttgtt
      901 gtaggcacac ggtttcaggt tctatttcac tccccttccg gggtgctttt cacctttccc
      961 tcacggtact ggttcactat cggtcactag ggagtattta gccttgggag atggtcctcc
     1021 cggattccga cggaatttct cgtgttccgc cgtactcagg atcctcctag gtgtcttcca
     1081 catttcgtct acggggtttt taccctcttt gactgacttt tccaagtcat tcgactatct
     1141 gaaagaacta ccatattgga gtcctacaac cccaacaagc aagcttgctg gtttgggctt
     1201 ttcccgtttc gctcgccgct actcagggaa tcgaattttc tttctcttcc tgcaggtact
     1261 tagatgtttc agttctctgc gtctacctcg aatgtgctat gtattcacac aat
//
LOCUS       DASHFF010000169         1312 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.181, whole genome shotgun sequence.
ACCESSION   DASHFF010000169 DASHFF010000000
VERSION     DASHFF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.181"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(156..1297)
                     /locus_tag="U2I75_002899"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="U2I75_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1697940.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          323 a          263 c          278 g          448 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc tt
//
LOCUS       DASHFF010000170         1298 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.182, whole genome shotgun sequence.
ACCESSION   DASHFF010000170 DASHFF010000000
VERSION     DASHFF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.182"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            143..400
                     /locus_tag="U2I75_002900"
     CDS             143..400
                     /locus_tag="U2I75_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697941.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="U2I75_002901"
     CDS             complement(632..1063)
                     /locus_tag="U2I75_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEN1697942.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          490 a          166 c          162 g          480 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt gttactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacatc ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa
     1141 catttgtatt ttatcaaagt tttattatac ttgtcaattt gtgtacatcc taaaatataa
     1201 gctatttgtt tacttctgta gaatatatac tttgttgtta gataagttgc acatgtttat
     1261 agaatctaaa taataaattt ttatgtaccg cccctcaa
//
LOCUS       DASHFF010000171         1264 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.183, whole genome shotgun sequence.
ACCESSION   DASHFF010000171 DASHFF010000000
VERSION     DASHFF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.183"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            34..>1264
                     /locus_tag="U2I75_002902"
     CDS             34..>1264
                     /locus_tag="U2I75_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1697943.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGF"
BASE COUNT          389 a          252 c          249 g          374 t
ORIGIN      
        1 taatttggct accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat
       61 accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa
      121 tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt
      181 tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg
      241 ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt
      301 aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt
      361 ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg
      421 acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga
      481 ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa
      541 ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc
      601 catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg ctatttttct
      661 cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat gtatgcgcct
      721 tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga tcgtttccac
      781 atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac cacggtacga
      841 cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa
      901 ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt
      961 aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta
     1021 cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca
     1081 ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact
     1141 ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca
     1201 aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc
     1261 tttc
//
LOCUS       DASHFF010000172         1246 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.185, whole genome shotgun sequence.
ACCESSION   DASHFF010000172 DASHFF010000000
VERSION     DASHFF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.185"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            200..742
                     /locus_tag="U2I75_002903"
     CDS             200..742
                     /locus_tag="U2I75_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697944.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="U2I75_002904"
     CDS             898..>1246
                     /locus_tag="U2I75_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN1697945.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DASHFF010000173         1238 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.186, whole genome shotgun sequence.
ACCESSION   DASHFF010000173 DASHFF010000000
VERSION     DASHFF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.186"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            144..542
                     /locus_tag="U2I75_002905"
     CDS             144..542
                     /locus_tag="U2I75_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1697946.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="U2I75_002906"
     CDS             526..1221
                     /locus_tag="U2I75_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEN1697947.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          482 a          179 c          253 g          324 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaa
//
LOCUS       DASHFF010000174         1212 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.187, whole genome shotgun sequence.
ACCESSION   DASHFF010000174 DASHFF010000000
VERSION     DASHFF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.187"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..559
                     /locus_tag="U2I75_002907"
     CDS             <1..559
                     /locus_tag="U2I75_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1697948.1"
                     /translation="KKFGAEKIFIEKQSGATITHRPIFQEALEFVRDQDIFIVEAIDR
                     LGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGNPHLDKFIKDLIIQILAMIAEQE
                     RTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQRRLVYENIVKDLNEGIAISKIA
                     KNYNITRQTIYRIKNDIEYNSNQMN"
     gene            complement(556..>1212)
                     /locus_tag="U2I75_002908"
     CDS             complement(556..>1212)
                     /locus_tag="U2I75_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697949.1"
                     /translation="QFLEELSGEEYRLFLNNNILKLISYAKKANSLSLVDNLDNLLPD
                     KLIYDENEDNSQKQIIIFELYLKWLSSLSVSPESNDDDKYFANKLFQFIFPDYSSMPF
                     FTLINFMLYVKEHLGYDDLVEMIKAYDKEPYPFKGINTGSDMAMISGETVEEILANYK
                     EQNIKTLELIKKNIKYYDPLLANKENIDKLLNAIADSDVRSPRIDELKEEILMYKELF
                     "
BASE COUNT          432 a          195 c          175 g          410 t
ORIGIN      
        1 gaaaaagttc ggcgcagaaa aaattttcat cgagaaacaa tctggagcaa cgattactca
       61 tcgaccgatt ttccaggaag ctttagagtt tgtccgagac caagatattt ttattgtcga
      121 agcaatcgac cgacttggaa gaaattacga cgaaatcatt gcttcagtca attacctaaa
      181 gaagaaaaac gtgcagctga tcatcaccag tctaccgatc atggctgaag caattggcaa
      241 tcctcatttg gataaattca ttaaagattt aatcatccaa attttggcca tgattgcaga
      301 gcaagagcgt accgaatcta aacgacgcca ggcgcaagtc attaaaattg caaaagcaaa
      361 tggcgtctat aaaggtcgtc caaaactcta tagtgcagat acaaaaaatc ctcaacgacg
      421 tttagtttat gaaaacatcg ttaaggattt aaatgagggc atagctattt caaaaattgc
      481 taaaaactac aatattacta gacaaactat ttacagaatt aagaatgata ttgagtataa
      541 cagtaatcaa atgaattaaa aaagttcttt atacattaaa atttcttctt ttaactcatc
      601 aattctagga gatctaacat cagaatcagc aattgcattt aataacttat caatattttc
      661 tttattcgct agtaaaggat catagtattt tatattcttt ttaattaatt ccagagtttt
      721 tatattttgt tctttgtaat tagctagaat ttcttctaca gtttcgcctg aaatcatagc
      781 catatctgaa ccagtattga ttcctttgaa tggataaggt tctttatcat atgcctttat
      841 catttctact aaatcatcat atcctaagtg ttctttaaca tacagcataa aatttattaa
      901 tgtaaaaaat ggcattgaag aatagtctgg aaatataaat tgaaacagtt tgttcgcaaa
      961 atatttatca tcatcattgc tttctggtga cactgacagg ctgctaagcc actttaaata
     1021 caattcaaat ataattattt gtttttgtga attatcttca ttctcatcat aaataagttt
     1081 atcgggtaaa agattatcta agttatctac taaagatagg ctattagctt tcttggcata
     1141 agatattaat tttaaaatat tattatttag gaataatcta tattcttctc cagataattc
     1201 ttctaaaaat tg
//
LOCUS       DASHFF010000175         1185 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.188, whole genome shotgun sequence.
ACCESSION   DASHFF010000175 DASHFF010000000
VERSION     DASHFF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.188"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..861
                     /locus_tag="U2I75_002909"
     CDS             <1..861
                     /locus_tag="U2I75_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697950.1"
                     /translation="HIHIHFAVVEKENTRKPYTEVENGVSYIVPKGKRRQATLDAMKT
                     NYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYDRHAMNLLNEVYK
                     TLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQISKALKEEAKSL
                     YGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVDQMQSIYNERSFR
                     TLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEKYHASKAYEEQQ
                     RRIQMEQERQ"
     gene            882..>1185
                     /locus_tag="U2I75_002910"
     CDS             882..>1185
                     /locus_tag="U2I75_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697951.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT          485 a          178 c          243 g          279 t
ORIGIN      
        1 catatccata ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc ttacacggag
       61 gttgaaaatg gtgtgagtta tatcgtaccg aaaggtaaga gaagacaagc aacattagac
      121 gcaatgaaga ccaattatgt acatgagtta gaacgttttt cttatgaaaa ggatcgaatg
      181 attggtatgg agaaacgaaa tttattgacg cggaaaagtg atttgcgtaa caccttaaca
      241 aaagtggtaa aagagccagc gcgttacgat cgacacgcga tgaacttatt aaatgaagta
      301 tataaaaccc tacctgacaa acgttcggaa tggaattacg gggatgaaaa acgaaccaaa
      361 ctttcaccat acacaagaga gaaactcgat gagttaacca aatatgtttt aacaaaagaa
      421 cctgaatatc aagaatatat acagatttca aaagcattga aagaagaagc aaaaagtctt
      481 tatggggaaa gcaagagaga atcaaaggat gcagagaaaa atgctctctt tgatttaaaa
      541 aagcgcacag gaaatgccat cctaacagag ctgaagagac aggatagtca aataaaacca
      601 ttaatagaaa gtgttgatca gatgcaatct atttacaatg aacgatcctt tcgaacgctt
      661 aaacaattgc caacgaatga atacaaatat agttacgaag aattcatgaa gcgcaggaaa
      721 caattccaac aaagagttgt cacgcagcgg agtttgcgtc aattaaatcg aacgattaat
      781 gcccatgaag aaaaatacca cgcaagtaaa gcatacgaag agcaacaacg aagaattcaa
      841 atggaacaag aaagacagta aagacagtag gaggaatagc aatgaaacaa tatttagatc
      901 aatggaaagt tatagaagga tcattaagag aagaacgaat cgaacaactt cctgattgtt
      961 tagaaaaaga gcatttgttc cagataagag aaatgttgag aaacgaacag tttgatccaa
     1021 atcaattttt agtcgttgag tacccagcta ctggtgtgta ttgttgtaat catgtgaatg
     1081 gtgaaaaata ttttatcatt caagaatatg agggcaagtt agcaccttat tatactactt
     1141 gggaaatgaa tgaagaggga atcaataact ttccatgtaa aagca
//
LOCUS       DASHFF010000176         1180 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.189, whole genome shotgun sequence.
ACCESSION   DASHFF010000176 DASHFF010000000
VERSION     DASHFF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.189"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..830)
                     /locus_tag="U2I75_002911"
                     /note="aph(2'')-Ia"
     CDS             complement(<1..830)
                     /locus_tag="U2I75_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HEN1697952.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     "
BASE COUNT          393 a          199 c          103 g          485 t
ORIGIN      
        1 ataaattcct gtttaatatt tttaattcca taaacaatag tttcaatagg ataatattct
       61 tcaactatat cttgatattc ttttgctttc tcaatatcta tatttccata cattcttaat
      121 atatcttctc caaaatttgt tcctatttct tcttcactat cttcaagtaa gtatataaaa
      181 tcacaatatt catctataat tccagaatct ccaaaatcaa ttattccagt taatctatta
      241 ttgccatcta acaatagatg attacaacta aaatcattat ggcataaaca ctttttaccc
      301 tcaaaaactg ttgttgcatt tagtctttcc ataaaacttt ctatataatc tttttctata
      361 tcagttaaat cattataaat agtttcacgc aacaatatat actcttctaa tacattttgt
      421 ttattatcaa tagtacattc actaatatct gtataatcta aaccgtgcat ttgtcttaaa
      481 aaactggcaa tatctcgttt taacaaattt tgttcttctt ctgacatagt agaataaatt
      541 tctggtgtta aaaaagttcc tttaatttct ttataaccta gtatagataa ttcatcacta
      601 atatacgaat attcaatatt aggaattttt acattagttt ctaaatttgt atttaaaaaa
      661 ttatatattg ctttttcttt tgcataacct tttttcttat tagtactaaa ttttgtttta
      721 aaaatgtatt cattattaac taaatatgcc acactatcat aaccactacc gattatttca
      781 atactatcta ctttgaaatt atcaaagtaa tgctcaatta aatatttcat tgccttaaca
      841 tttgtggcat tatcatcata tctatattcc attaaataac aatcttcttt tttgccctcg
      901 tgtaattcat gttctggcaa atcttcaata attctaaaac cagatttttg gtatgccctt
      961 attgctcttg gattattttt atgagggtct aaaataactg cattagcatt tctttctttt
     1021 ttcaaaaatt caaaaatcaa tttaatatat cttgtacggt tctgttgcaa agttttaaat
     1081 aaagaataaa atcccttacg gtatctatga tttaagctgg gattcccaat aataccttga
     1141 tttcagtaca gaccgaaaac ccgaagagag tgccttcttt
//
LOCUS       DASHFF010000177         1147 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.190, whole genome shotgun sequence.
ACCESSION   DASHFF010000177 DASHFF010000000
VERSION     DASHFF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.190"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            177..971
                     /locus_tag="U2I75_002912"
     CDS             177..971
                     /locus_tag="U2I75_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEN1697953.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT          449 a          139 c          185 g          374 t
ORIGIN      
        1 aattatactg tttcctgata agctctcaat tcttctgaac ccatgatgtt agtgcttttc
       61 ttctattatt aaatatcttg gaattttcta ttctttctaa aaagtatggt caaagtatgg
      121 tcactattga tagaatgtta atagtagtag aaagctgata tctaggagga actaatgtga
      181 gtaaacaatg tgcaaagtgc ggaggtaaaa ttggcttaac ttcatataaa atcaaagata
      241 aacaaattgt ttgcggcaat tgtatgaaaa aagctggtta cggtatgact actccttata
      301 aaataataag atctttgaat ttagaagatt tagataagga tccatcagaa aaaagaaaat
      361 taaacatatc tgatcatact gcacaaaaca atcatgatat aggtaatgat agaatgagaa
      421 ccactgaaga gatggtagaa ttttgtctta aatacggcta cggtaaagga atgactaaaa
      481 aatggacaac tcatcatttt aacttaatat cagatcaact taacagtgac gaatacgtaa
      541 tcttctgctt tgtaggattg cataattata tctcatcaac taagcatgac aataattatg
      601 catacgcatt aactaataaa cgtttaattg tagctcaaca aaaaatgata ggcaataatg
      661 tacaaagtat tatattagac aatttaaata atattagtaa aaaaagagga gtgctgttgg
      721 gtacattaac tattgatact cttggtaaag tattgaatgt tgcagttgat aaagatactg
      781 ttgataggat aagtgattct ttaaacgaaa ttatttataa tttaaaaaga agaaatattt
      841 ctgctacaaa ttccaataca tcaacaattt cctcagcttc agaaataaga aaatataaag
      901 aactattgga tgatggaatc attacagaag aagaatttaa taaaaagaaa caagaattat
      961 tagatttata atatctatca aagaagatgg gcatttaatt tagctcatct ttttctattt
     1021 acaatacaaa catttgttcg tatactcttt ttgaggtgaa ctttatatgt taatggaagg
     1081 aaaaacacaa ttatggttta aattcgatcc ttcgaataga tttgtcaaag atttttataa
     1141 ggtgtgg
//
LOCUS       DASHFF010000178         1112 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.192, whole genome shotgun sequence.
ACCESSION   DASHFF010000178 DASHFF010000000
VERSION     DASHFF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.192"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            154..417
                     /locus_tag="U2I75_002913"
     CDS             154..417
                     /locus_tag="U2I75_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1697954.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="U2I75_002914"
     CDS             417..689
                     /locus_tag="U2I75_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1697955.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="U2I75_002915"
     CDS             766..1095
                     /locus_tag="U2I75_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1697956.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          423 a          141 c          220 g          328 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa ac
//
LOCUS       DASHFF010000179         1095 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.193, whole genome shotgun sequence.
ACCESSION   DASHFF010000179 DASHFF010000000
VERSION     DASHFF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.193"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>1095)
                     /locus_tag="U2I75_002916"
     CDS             complement(<1..>1095)
                     /locus_tag="U2I75_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEN1697957.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRL
                     TDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATW
                     LWKGKEPLFFCSKIRNEVDKKLFQARVTSL"
BASE COUNT          295 a          230 c          194 g          376 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat gactcgttga tgatttttac ttatggtaca ttggtttagc
      361 agaggacagt tcacacattc ttttggattg gatttgtatt ctcggtaccc ttctcgagta
      421 accgttgaaa aagcgagcat ttgatcttgc ggacaaatat aacaatcaaa atactcgtca
      481 tagagaaact cattttttga taatagctcc tttttccctt tgggccttga ataaggcaaa
      541 caaggtcgaa ggttcaaatg agtcaaaaag tgaacaaact taggtgtctt atacccagca
      601 tcagcgacaa cacaattgat ttcaggaaaa agtctcttcg attgtttgac gagctgaaag
      661 gcgacttgac tgtcatgaat atttctagga gtgatgattg tgctaagaat aaatccattg
      721 tcgtcgcatg aagtatggac agaataagca aattgttttt ctcgttcgcc tttgacgtag
      781 tagccgcttt ccggatcagc tttacttatt tttctttctt tcagcttcac ttccttttct
      841 aagtccaaag taaaagggcg ttttccagcc tctattcgtt gacgattgat ctcatcttct
      901 aattcatcct gataggcttt cgcttcatca tgggtcattt ctttcgtaaa cttatgttta
      961 ttggcgttag ctttgatgtg ggtagaatcc agatacaaat tatcctctgt tacaaaacct
     1021 gctttaactg cttgttctaa aatataggca aaaatatcct caaacaccgt cgtttctcta
     1081 aatcggcgaa cataa
//
LOCUS       DASHFF010000180         1062 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.194, whole genome shotgun sequence.
ACCESSION   DASHFF010000180 DASHFF010000000
VERSION     DASHFF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.194"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(37..993)
                     /locus_tag="U2I75_002917"
     CDS             complement(37..993)
                     /locus_tag="U2I75_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697958.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DASHFF010000181         1038 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.195, whole genome shotgun sequence.
ACCESSION   DASHFF010000181 DASHFF010000000
VERSION     DASHFF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.195"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>1038
                     /locus_tag="U2I75_002918"
     CDS             <1..>1038
                     /locus_tag="U2I75_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1697959.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKH"
BASE COUNT          361 a          204 c          187 g          286 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacacc
//
LOCUS       DASHFF010000182         1000 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.196, whole genome shotgun sequence.
ACCESSION   DASHFF010000182 DASHFF010000000
VERSION     DASHFF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.196"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            369..1000
                     /locus_tag="U2I75_002919"
                     /pseudo
     CDS             369..1000
                     /locus_tag="U2I75_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          343 a          181 c          182 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccactact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DASHFF010000183          969 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.198, whole genome shotgun sequence.
ACCESSION   DASHFF010000183 DASHFF010000000
VERSION     DASHFF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.198"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            40..>969
                     /locus_tag="U2I75_002920"
     CDS             40..>969
                     /locus_tag="U2I75_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1697960.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYV"
BASE COUNT          346 a          175 c          186 g          262 t
ORIGIN      
        1 aaatagttcc gacgaaagaa ttataaggag tgagtacaaa tgacctataa ccatcttaca
       61 ccaactgaac tcgtaatgat agaagcttat ttcaatcaat cacaacctgt ttcaaaagta
      121 gccaatctgt tacaacgttc tcgacaaact atttataagg tctatcgttt tcttaaatct
      181 ggtggaagcg caatgagcta ctataaacaa tataagaaaa acaaacgtaa ctgcggaaga
      241 catccgattg ttttacctga tgagcaacag gaatacattc aaaaaaaggt tgttcaaggg
      301 tgggctcctg atgtccttat tggtcgagca gagtttccta ttgcttgttc ggtacgcaca
      361 ctgtatcgaa tgtttaaaga taaaaccttt gacactcacg atttaccaat gaagggaaaa
      421 agaaaaccaa acggtcataa agaaaaaaga ggaaaacaag ggtttcgccg gtcaatccga
      481 gacagaaaga cagactatac ccagtttgat catgaatttg gtcacctgga aggtgacact
      541 atcgtgggtg gaaaacataa aagcgcggtc atcacattag ttgaacggct atctaaagtc
      601 atcattacct tgaaacccga aggcagacag gctaaagaca ttgaaaatag gctgaatcaa
      661 tggtttcgtt gtgtcccaaa aaacttattt aaatccatca catttgattg tggaaaagaa
      721 ttctcaaatt ggaaaaagat tagtaatacc aacgatattt caatttattt tgcggatcca
      781 ggaacacctt ctcaacgagg attgaatgaa cattcaaatg gcttattacg taaagatggt
      841 ttgtataaag ctatggattt taatggtgta tctgaaacct ttatccaatc ggtggcatcg
      901 aaacgaaatc atattcctag aaaatcatta cactatcgga caccaatgga agtatttttg
      961 agttacgtc
//
LOCUS       DASHFF010000184          934 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.199, whole genome shotgun sequence.
ACCESSION   DASHFF010000184 DASHFF010000000
VERSION     DASHFF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.199"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            162..740
                     /gene="pgsA"
                     /locus_tag="U2I75_002921"
     CDS             162..740
                     /gene="pgsA"
                     /locus_tag="U2I75_002921"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN1697961.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
BASE COUNT          262 a          162 c          211 g          299 t
ORIGIN      
        1 aaggatcgtg acaaatctgt gtcgcggtcc ttcttcgtta attcaaaaag atcaatttca
       61 ataaaaatac tcctttgaat aaattcattt ggaaaagtaa ataatatcgg tggaaacttc
      121 gctgatagtc acgtagaata gggaagaaag gagtcgatag tgtgaattta ccaaataaat
      181 taactgtact tagaatttgt atgatcccga ttttcatttt ggttgtaggt ttacctttag
      241 attggggaca atttaacgta ggagacacca cactggctgt cacacagcta gtaggtgcag
      301 ttatttttgc tttagctagt atcacagact ggttagatgg aaaaattgcc cgtgcgcgag
      361 gccttgtcac taattttggc aagtttgcgg acccgttggc agataaaatg ctagttatga
      421 ctgcttttat catgttggtt gaccaaggaa aggcccctgg ttgggtagta gcgatcattg
      481 tctgtcgtga attagccgtc acaggcttgc gtttactact tgtcgaacat ggagaagtca
      541 tggctgcagc ttggccggga aaagtcaaga cggctacaca aatggttgct attatcttgc
      601 tgtttatcaa taatatccca ttcagcctga tcggtattcc agtagatcaa atcatgcttt
      661 atgcttgtct gatctttact atctattcag gtgtggatta cttcatcaaa aacagcggtg
      721 tgttcaaagg atcaatgtaa gtatcgttta gtggagaggt tgtgagcctg ccgtctaact
      781 ctgagtcaca agggataatt tcaaaaaaca gcttttcatg ttttatgtat attgtgactt
      841 atgaccgagg ttttggcagg agaacactgt ttatttggaa caaggagctg tgacaagatt
      901 tttgtcacag ctccttgccc tctttttaaa taaa
//
LOCUS       DASHFF010000185          932 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.200, whole genome shotgun sequence.
ACCESSION   DASHFF010000185 DASHFF010000000
VERSION     DASHFF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.200"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>932
                     /locus_tag="U2I75_002922"
     CDS             <1..>932
                     /locus_tag="U2I75_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1697962.1"
                     /translation="TIWSALGEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQ
                     ELNGKIAELKDSLAPIGNTIIDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAA
                     FGILLPAILAVSYLFGPMMLIIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLG
                     DTWESIKDGASSVWDGVKETWSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQ
                     ETWQTFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAG
                     QLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNG"
BASE COUNT          273 a          134 c          232 g          293 t
ORIGIN      
        1 acaatttggt ctgcgcttgg cgaagataat gcgatgaaag ttgtccaatc tttaactgat
       61 gttaaccata catttgatga tgttagtgga tctgcacaaa aaatgaatga agattctact
      121 actccgttgc aagagttgaa cgggaaaata gctgaattaa aggattcatt agctcctata
      181 ggcaacacaa tcatagatgc actcgaacca gtaattgatt ttctaggaaa gatggctgat
      241 gcgtttaata atcttccaca accagtacag gatttcattg ttgctatagg tggtcttaca
      301 gcagcatttg gcatactgtt accggcaata ctagccgtgt catatttatt tggtccgatg
      361 atgcttataa ttggaggaat tatagctgtt atagcaggcg ttattgtggc aattaagaac
      421 tggggtgcaa ttactgactg gtttagtggt ttatggaaaa aatttactga ttggttgggt
      481 gatacttggg aaagtataaa agacggagcc tcatcagttt gggatggggt taaagaaacc
      541 tggtctggat ttgtagattg ggttcaagat atttggcaag gagtttctga ttggtttgga
      601 gagctatgga gcggattagt tgaaggagct tccaacatct ggcaaggagt ccaagaaact
      661 tggcaaacat tcgttgattg ggtttcaaat atttggaacg gagtcaaaga agtatggtcg
      721 attatttggg cagacattgt aggaattgtt caaataccat ggacattaat aacgtcattg
      781 attcaagctg gtattaatat tatcgtgggt atttttgatg tagctggaca gttattgggc
      841 gcagcttggc aagctgtttg gacacctatt tctgatttcc ttaaaaatac ttgggatact
      901 atgacacaat ggataagtat cgcttggaat gg
//
LOCUS       DASHFF010000186          901 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.201, whole genome shotgun sequence.
ACCESSION   DASHFF010000186 DASHFF010000000
VERSION     DASHFF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.201"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            46..105
                     /gene="ermCL"
                     /locus_tag="U2I75_002923"
     CDS             46..105
                     /gene="ermCL"
                     /locus_tag="U2I75_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HEN1697963.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            165..899
                     /locus_tag="U2I75_002924"
     CDS             165..899
                     /locus_tag="U2I75_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HEN1697964.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          378 a          110 c          118 g          295 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat
      121 tgttaatata aacattcatt ataacctcat aggagtgggt tataatgaac aaaaaaaata
      181 taaaagatag tcaaaacttt attacttcaa agcatcatat aaatgaaatt ttgagaaatg
      241 tacatttaaa tacaaatgat aatattattg agattggttc agggaaaggt catttcacgt
      301 ttgaattagc taaaaggtgt aattatgtaa ccgccattga aatagatcct aaattatgta
      361 ggataactaa aaacaaactt attgaatatg agaacttcca ggttatcaat aaagatattt
      421 tacaatttaa gtttcctaaa aataagtcat ataagatata tggaaatata ccctacaata
      481 taagtacaga tataattcga aaaattgttt ttgaaagcac agctacagaa agttatttaa
      541 tagtggaata tggatttgct aaaaggttgc taaatacaaa tcgttcacta gcactatttt
      601 taatgacaga agttgatata tccatattaa gtaaaatccc tagagaatac tttcatccaa
      661 aacctagagt taatagctcg ttaattgtat taaaaagaca cccttcaaaa atatcactca
      721 aagatagaaa acaatatgaa aattttgtta tgaaatgggt taacaaagaa tacataaaac
      781 tattttccaa aaaccaattt tatcaagcct taaaatatgc aagaattgac gatttaaaca
      841 atattagctt tgaacaattc ttgtctcttt tcaatagcta taaattattt aatagataag
      901 t
//
LOCUS       DASHFF010000187          883 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.202, whole genome shotgun sequence.
ACCESSION   DASHFF010000187 DASHFF010000000
VERSION     DASHFF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.202"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..146)
                     /locus_tag="U2I75_002925"
     CDS             complement(<1..146)
                     /locus_tag="U2I75_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697965.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGR"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="U2I75_002926"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="U2I75_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN1697966.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..785)
                     /locus_tag="U2I75_002927"
     CDS             complement(591..785)
                     /locus_tag="U2I75_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697967.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          253 a          174 c          158 g          298 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta
      781 tacatagaag acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag
      841 taaggtactg ttctatgggg cgcttacgtt aaaaatgttt tgc
//
LOCUS       DASHFF010000188          876 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.203, whole genome shotgun sequence.
ACCESSION   DASHFF010000188 DASHFF010000000
VERSION     DASHFF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.203"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            269..>876
                     /locus_tag="U2I75_002928"
     CDS             269..>876
                     /locus_tag="U2I75_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697968.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVT"
BASE COUNT          302 a          130 c          163 g          281 t
ORIGIN      
        1 aattttttct ataagttata ttatcataat accacaaaat cacaaatttt cattcaatta
       61 tcattttcaa ttgcatttta taaatcattt tgtatgtaat ttggtatgta agttaaaatg
      121 aaaaaagtag tccattttga agtacatttt gtcattttgt actccaaaac tgaactactt
      181 acttgcatat atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc
      241 agaaaaagaa aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa
      301 gattgataga gcttattctg gagaaattgt tattttgcaa aatgagtttt tgaagttaaa
      361 tagtgttctt ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc
      421 tctactacaa acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc
      481 ccttttggaa atctcagata gtgatccgct tctacgatat tacgtggatt ctacgacaca
      541 tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca
      601 agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc
      661 gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc
      721 cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt
      781 ttctcttgga tacttaaatc aatcatttca aaatgcagtt atggaaggga tacgctatgg
      841 ttgcgaacaa ggattatatg gttggaatgt gacgga
//
LOCUS       DASHFF010000189          870 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.204, whole genome shotgun sequence.
ACCESSION   DASHFF010000189 DASHFF010000000
VERSION     DASHFF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.204"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            39..>870
                     /locus_tag="U2I75_002929"
     CDS             39..>870
                     /locus_tag="U2I75_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1697969.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          301 a          151 c          167 g          251 t
ORIGIN      
        1 aatagttccg accaaagaat tataaggagt gagtacaaat gacctatacc catcttacaa
       61 cggatgaact tgtaataata gagtcttatt tcaaaatgaa tcaatctgtt gctaaaactg
      121 cccattgctt gaatcgttca agacaaacga tccataaagt atatctgttc ttcaagcaag
      181 gaaaatcagc cttagaatat tatcaacagt ataagaaaaa caaatcaaac tgtggtagac
      241 gtccgcttgt tttacccgag gaacaatcag aatatattca aagaaaggtt gttcaaggat
      301 ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat tagttgttct gctcgtacca
      361 tttatcgtat gttcaaaaag gggctatttg attcttctga cttaccgatg aaaggcaagc
      421 gtaaaccgaa tggacatcaa gaaagacgtg gaaaacaaac tttccgccgc tctattcatg
      481 aacgtgaaaa ggattatagc caattctcaa atgagtttgg tcaccttgaa ggtgacacta
      541 tcgtaggtct gaaacataaa agtgctgtaa ttaccttagt tgaacgatta tcaaaagtta
      601 tcatcacatt gaaaccgtgt ggtagacaag cgattgatat tgaaaaaaaa ttaaatcaat
      661 ggtttgaatc tgtaccgaaa aacctattca aatccatcac ttttgattgt ggaaaggaat
      721 tttcaaattg gaaacagatc agtaatgtca atgatattgc catttatttc gctgatccag
      781 gaacgccgtc tcaaagaggc ctaaacgaga attctaacgg attgttacgt agagatggtt
      841 tattgaaatc tatggatttc aattcagtag
//
LOCUS       DASHFF010000190          863 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.205, whole genome shotgun sequence.
ACCESSION   DASHFF010000190 DASHFF010000000
VERSION     DASHFF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.205"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..861
                     /locus_tag="U2I75_002930"
     CDS             <1..861
                     /locus_tag="U2I75_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEN1697970.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DASHFF010000191          811 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.208, whole genome shotgun sequence.
ACCESSION   DASHFF010000191 DASHFF010000000
VERSION     DASHFF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.208"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            178..774
                     /locus_tag="U2I75_002931"
     CDS             178..774
                     /locus_tag="U2I75_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1697971.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          233 a          122 c          150 g          306 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg t
//
LOCUS       DASHFF010000192          793 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.209, whole genome shotgun sequence.
ACCESSION   DASHFF010000192 DASHFF010000000
VERSION     DASHFF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.209"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..672
                     /locus_tag="U2I75_002932"
     CDS             <1..672
                     /locus_tag="U2I75_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1697972.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          292 a          123 c          148 g          230 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgt
//
LOCUS       DASHFF010000193          791 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.210, whole genome shotgun sequence.
ACCESSION   DASHFF010000193 DASHFF010000000
VERSION     DASHFF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.210"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..780
                     /locus_tag="U2I75_002933"
     CDS             <1..780
                     /locus_tag="U2I75_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1697973.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          338 a          107 c          102 g          244 t
ORIGIN      
        1 gatatgctag attactacta ttttgaaaaa aatttatcat ataattatgt aatacattta
       61 aaaactttta caggaatgat ttttaaattc gccaaccgtc gatatggatt aaaatataat
      121 cccgtaaatg gggtttcttt agcaagaaaa cctaagacaa acgaagaaat aaaaaaagaa
      181 aaagatggtt atttatcaaa agaagaagtt caaatgattg ctagtaaaca aaaaagttca
      241 catcaacagt cacgttattc tttaattact aaatttttgt ttttaactgg actacgttat
      301 ggtgaattga tatctttgac tgaatctgat tatgatggta ataaaattac ggtttctggt
      361 acttatgatt acgagttaaa aattaaaaca acaactaaaa atacaggttc ttatagaacc
      421 attgaattat caagtaatgc taaagaaatc atagatcaat taattgaaga aaacaaatta
      481 attaagaata ataaagataa atacattttc atctctaaaa atggtaaccc gatatctatt
      541 caagcatata atcaatcttt aagagcagta tccaaagaat taaacataga taaaaaagtt
      601 tcttctcaca tgttaaggca ttcccacatt tctctactta cagaattagg aatcccatta
      661 aaagccatta tggatcgtgt aggacatgaa gattcaaaaa caacattaaa gatttatact
      721 catacaacta aaaatatgca aaatcaacta gtagaaaaac taggaaaaat agaaatttaa
      781 attgcccctt t
//
LOCUS       DASHFF010000194          772 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.211, whole genome shotgun sequence.
ACCESSION   DASHFF010000194 DASHFF010000000
VERSION     DASHFF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.211"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(109..>772)
                     /locus_tag="U2I75_002934"
     CDS             complement(109..>772)
                     /locus_tag="U2I75_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEN1697974.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          213 a          178 c          149 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gc
//
LOCUS       DASHFF010000195          764 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.212, whole genome shotgun sequence.
ACCESSION   DASHFF010000195 DASHFF010000000
VERSION     DASHFF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.212"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(191..589)
                     /locus_tag="U2I75_002935"
     CDS             complement(191..589)
                     /locus_tag="U2I75_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1697975.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT          260 a          137 c          103 g          264 t
ORIGIN      
        1 caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca attaaaaaaa
       61 gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa aaaaaataga
      121 ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa acgtcagatt
      181 agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct agatcttgga
      241 cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg aaagtaccac
      301 cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcattttca acactaaacg
      361 aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt ataaattttt
      421 tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc attgaaccag
      481 cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa aaagcaacgg
      541 aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt tgtttcgtct
      601 cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa aatttagatt
      661 catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg tgatagaatg
      721 aggactggtt cactttcact taactaatag atggaatttc ttta
//
LOCUS       DASHFF010000196          755 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.213, whole genome shotgun sequence.
ACCESSION   DASHFF010000196 DASHFF010000000
VERSION     DASHFF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.213"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            660..>755
                     /locus_tag="U2I75_002936"
     rRNA            660..>755
                     /locus_tag="U2I75_002936"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          309 a          123 c          131 g          192 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgtgcctaa tacatgcaag
      721 tcgaacgctt ctttttccac cggagcttgc tccac
//
LOCUS       DASHFF010000197          713 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.214, whole genome shotgun sequence.
ACCESSION   DASHFF010000197 DASHFF010000000
VERSION     DASHFF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.214"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..448)
                     /locus_tag="U2I75_002937"
     CDS             complement(<1..448)
                     /locus_tag="U2I75_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697976.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          123 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aac
//
LOCUS       DASHFF010000198          696 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.215, whole genome shotgun sequence.
ACCESSION   DASHFF010000198 DASHFF010000000
VERSION     DASHFF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.215"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(53..628)
                     /locus_tag="U2I75_002938"
     CDS             complement(53..628)
                     /locus_tag="U2I75_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1697977.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          125 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaacg
//
LOCUS       DASHFF010000199          672 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.216, whole genome shotgun sequence.
ACCESSION   DASHFF010000199 DASHFF010000000
VERSION     DASHFF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.216"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          231 a           93 c          138 g          210 t
ORIGIN      
        1 acggtatgtg gttaaaaaag aaaaatagct aggagatttt tgagagatga ctggtaaatt
       61 taaaaatgat agaaatggag agtatgtatg agtaataaac aatttttatg gtcttctttt
      121 gcatttattg ttgttgatat ttttttccga tattttaata acgatatgat atttaactgg
      181 caaagaatta taatcgatta ctgtattttt tgtataatat attttttagt gaaaacattt
      241 ctaaaatacg caaaaaaata aaattgaaga tgttattaaa tcgcgattat ttacggagat
      301 ttatataaat aaaaaccccc tgacgaagtc gaagggggtt tttattttgg tttgaggttt
      361 gcatacctac cgaaaaggta gggcagcaaa tcgtcaaaca ggtatcagct aattgcacca
      421 taagggtgcg ctgatactcg gctcaaaaga gccgaaagag ccgtctgcaa gacccctcgg
      481 cgagcccaca ctttgcttgc aaagtatagt gggttatact ttacatggaa gtaattgccg
      541 gaaatagtga aaatgcctca catttgtgcc acctaaaaag gagcgattta catatgagtt
      601 atgcagttta cgggccaaga ttgtttaagc aaaataagat aagttaaggg atgcagttta
      661 tgcacccctt aa
//
LOCUS       DASHFF010000200          639 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.217, whole genome shotgun sequence.
ACCESSION   DASHFF010000200 DASHFF010000000
VERSION     DASHFF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.217"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..282
                     /locus_tag="U2I75_002939"
     CDS             <1..282
                     /locus_tag="U2I75_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1697978.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          213 t
ORIGIN      
        1 ttaatacaag atgatttaca ccatacgcat cggttatggt taatgaataa aattaatctg
       61 tattttgggc attcttatgt ttttgctttt tatgattaca ggacaaatat aactgaagta
      121 gatttggtta tttctaatta ctacatcgac actggaaaaa cgccattact gttaatgaaa
      181 aatataccta ccgaaagaaa ctggcgtttg ttcgagaaaa cgatttatca attgaaaaaa
      241 gaaaaagagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa acggaaaaat
      301 agtttttcat attttgccgt ttttgagcta ataccgaggg acagctgatt gaacaccatt
      361 tattgagaga ttgtgaaaaa actgtcctgc atcaagtaat aagaacagcc aagtaaaaac
      421 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcaa gagaacaccg
      481 tttattcgga aaaagggact gtgacactac atgtcacagt ccctttttga ttgaattatc
      541 tcatttgttt tctaattttt tctttatttg agaaaatagt tgaactggat aaaaagtact
      601 ggacgtgtca aaattatttt ggatcaaatt agatctatt
//
LOCUS       DASHFF010000201          617 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.218, whole genome shotgun sequence.
ACCESSION   DASHFF010000201 DASHFF010000000
VERSION     DASHFF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.218"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>617
                     /locus_tag="U2I75_002940"
     CDS             <1..>617
                     /locus_tag="U2I75_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEN1697979.1"
                     /translation="KCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVSCGMRT
                     LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEG
                     DTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFD
                     CGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGL"
BASE COUNT          203 a          117 c          117 g          180 t
ORIGIN      
        1 aaaaatgtgg tagaaaagtc attcaattac ctgctcatga agtagattac attaaagaga
       61 aagtcactct tggttggacg cctgacgtca ttatcgggcg aaaagaaagg tctgtttcat
      121 gcggtatgag aacactttat cgtttatttt ctaaaggaat atttgatatt gacacactac
      181 cgatgaaagg taaaagaaaa cccaatggcc atcaggaaaa acggggaaaa caacagtatc
      241 agcgctcaat ccatgatcga cctgataatt atcctgattt caattctgag tttggtcacc
      301 ttgaaggtga tacgatcgtt ggcattcatc ataaaagtgc cgtcattact ttagttgaaa
      361 gattatctaa agtcattatc acgattaaac ccaacggccg taaggcatta gatattgaaa
      421 ctgcccttaa tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct attacgtttg
      481 actgtggaaa agaattttct aactggaaag ccattagtaa ccaacatgat attgatatat
      541 attttgcgga ccctggaaca ccttctcaac gcccattaaa cgagaattct aacgggattc
      601 tgcgtcgtaa tggactg
//
LOCUS       DASHFF010000202          597 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.219, whole genome shotgun sequence.
ACCESSION   DASHFF010000202 DASHFF010000000
VERSION     DASHFF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.219"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..355
                     /locus_tag="U2I75_002941"
     CDS             <1..355
                     /locus_tag="U2I75_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697980.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
BASE COUNT          227 a           72 c          120 g          178 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaact
//
LOCUS       DASHFF010000203          556 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.220, whole genome shotgun sequence.
ACCESSION   DASHFF010000203 DASHFF010000000
VERSION     DASHFF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.220"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>556
                     /locus_tag="U2I75_002942"
     rRNA            <1..>556
                     /locus_tag="U2I75_002942"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          135 a          130 c          168 g          123 t
ORIGIN      
        1 catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa ggcggctctc
       61 tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt agataccctg
      121 gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc ttcagtgctg
      181 cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa actcaaagga
      241 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa
      301 ccttaccagg tcttgacatc ctttgaccac tctagagata gagcttcccc ttcgggggca
      361 aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc
      421 gcaacgagcg caacccttat tgttagttgc catcattcag ttgggcactc tagcaagact
      481 gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct
      541 gggctacaca cgtgct
//
LOCUS       DASHFF010000204          548 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.221, whole genome shotgun sequence.
ACCESSION   DASHFF010000204 DASHFF010000000
VERSION     DASHFF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.221"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>548)
                     /locus_tag="U2I75_002943"
     CDS             complement(<1..>548)
                     /locus_tag="U2I75_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN1697981.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DASHFF010000205          537 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.222, whole genome shotgun sequence.
ACCESSION   DASHFF010000205 DASHFF010000000
VERSION     DASHFF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.222"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..365
                     /locus_tag="U2I75_002944"
     CDS             <1..365
                     /locus_tag="U2I75_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1697982.1"
                     /translation="VLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGD
                     AVDQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELF
                     ISILKELEEHENWYAEVE"
     gene            366..>537
                     /locus_tag="U2I75_002945"
     CDS             366..>537
                     /locus_tag="U2I75_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697983.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          211 a           78 c          117 g          131 t
ORIGIN      
        1 tcgtcttaca gacccaagta aaatcgatga gaaatgcgat gcaatatctt gagtatatgg
       61 acttttttgt gaaagaattt gatacaaatt tatatgttgc tggtgttatt cctttcatgt
      121 tggaaagcgg tgacgcagta gatcaggaaa tgtataaaca agcccaagag atttatgggg
      181 aacatttgat taaaaatgtt gtactcaaaa atgcgcgctt aaaacgatac gacggatcag
      241 gtattacgat ggagaagaca aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg
      301 aattatttat cagtattttg aaagaattgg aagaacacga gaattggtat gcggaggtgg
      361 agtaattggc tgacatcaaa ggacttgata tttttgggaa caacaaagga ctcgccaaaa
      421 taaaaaaaga agcagaagca aagaaaaagc agcaagcaat tacaccatct gaaaataagc
      481 aattagaagt gcaccaagtt caagaaataa aaacttcctc caatgttgaa agacaga
//
LOCUS       DASHFF010000206          529 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.223, whole genome shotgun sequence.
ACCESSION   DASHFF010000206 DASHFF010000000
VERSION     DASHFF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.223"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            7..302
                     /locus_tag="U2I75_002946"
                     /pseudo
     CDS             7..302
                     /locus_tag="U2I75_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            377..>529
                     /locus_tag="U2I75_002947"
     CDS             377..>529
                     /locus_tag="U2I75_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1697984.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          200 a           63 c          112 g          154 t
ORIGIN      
        1 atgtaggtga aaaagtttaa aaagaaaata tatttaggat tggttgttgg ggtgagtatg
       61 tttgggttca tggctcatac taaacaagta actgcagata ctaaccaaaa agaaaggatt
      121 atgagccaat tagatgactc tcaaataagt atgtacccaa tgagctaatt gtcagtttaa
      181 aggctacaca aagaatttca acagagaaaa ataaaccgca aaatttattg ggtgtaaaag
      241 ttatggtgga aaaagatttg actcttagga caaatgaaag cgattgtaat aagcgagatc
      301 aagggttctg ttgcaaagtt ttccaaaaaa tctattttag tgtaaaattg agaaaaaaga
      361 cagagaggac agagtaatga atcattttaa aggcaaacaa ttcaaaaaag acgtcattat
      421 tgtcgctgtt ggttactacc tgcgttacaa tctaagctat cgtgaagttc aggaattgtt
      481 atatgatcgt ggaataaatg tttgtcatac tacgatttat cgttgggta
//
LOCUS       DASHFF010000207          528 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.224, whole genome shotgun sequence.
ACCESSION   DASHFF010000207 DASHFF010000000
VERSION     DASHFF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.224"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(5..90)
                     /locus_tag="U2I75_002948"
     tRNA            complement(5..90)
                     /locus_tag="U2I75_002948"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="U2I75_002949"
     tRNA            complement(107..178)
                     /locus_tag="U2I75_002949"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="U2I75_002950"
     tRNA            complement(190..262)
                     /locus_tag="U2I75_002950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="U2I75_002951"
     tRNA            complement(280..361)
                     /locus_tag="U2I75_002951"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="U2I75_002952"
     tRNA            complement(369..441)
                     /locus_tag="U2I75_002952"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="U2I75_002953"
     tRNA            complement(445..517)
                     /locus_tag="U2I75_002953"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DASHFF010000208          523 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.225, whole genome shotgun sequence.
ACCESSION   DASHFF010000208 DASHFF010000000
VERSION     DASHFF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.225"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(152..346)
                     /locus_tag="U2I75_002954"
     CDS             complement(152..346)
                     /locus_tag="U2I75_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697985.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT          163 a          110 c           79 g          171 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caa
//
LOCUS       DASHFF010000209          517 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.226, whole genome shotgun sequence.
ACCESSION   DASHFF010000209 DASHFF010000000
VERSION     DASHFF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.226"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            57..>517
                     /locus_tag="U2I75_002955"
     CDS             57..>517
                     /locus_tag="U2I75_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1697986.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DASHFF010000210          513 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.227, whole genome shotgun sequence.
ACCESSION   DASHFF010000210 DASHFF010000000
VERSION     DASHFF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.227"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..132)
                     /locus_tag="U2I75_002956"
     CDS             complement(<1..132)
                     /locus_tag="U2I75_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1697987.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          185 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag aca
//
LOCUS       DASHFF010000211          511 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.228, whole genome shotgun sequence.
ACCESSION   DASHFF010000211 DASHFF010000000
VERSION     DASHFF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.228"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            15..>511
                     /locus_tag="U2I75_002957"
     CDS             15..>511
                     /locus_tag="U2I75_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HEN1697988.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          185 a           93 c           85 g          148 t
ORIGIN      
        1 aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta gtcactaaag
       61 gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca ctcctacgga
      121 ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt aattcaggta
      181 attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa ttaacattgg
      241 aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc tacaaaagaa
      301 caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt acgatgtctg
      361 aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa accatgtcca
      421 acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc ttaaatgata
      481 agtatgtcgc tatttttatg gacgctactt a
//
LOCUS       DASHFF010000212          500 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.229, whole genome shotgun sequence.
ACCESSION   DASHFF010000212 DASHFF010000000
VERSION     DASHFF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.229"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>500
                     /locus_tag="U2I75_002958"
     CDS             <1..>500
                     /locus_tag="U2I75_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HEN1697989.1"
                     /translation="SLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFK
                     IEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEI
                     VILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDS
                     DPLLQY"
BASE COUNT          179 a           82 c          100 g          139 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta
//
LOCUS       DASHFF010000213          474 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.230, whole genome shotgun sequence.
ACCESSION   DASHFF010000213 DASHFF010000000
VERSION     DASHFF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.230"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>474)
                     /locus_tag="U2I75_002959"
     CDS             complement(<1..>474)
                     /locus_tag="U2I75_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697990.1"
                     /translation="NEAQSLMEHGINFDKLEEQDFTKYLSYMTRHYALEQKKELTQRE
                     QKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEVKGSDLKEIKQIFQRGQQKGSI
                     IYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRKMMETLFEKEQLNETGYWMA"
BASE COUNT          108 a           97 c           77 g          192 t
ORIGIN      
        1 gccatccagt aacctgtttc attcaattgt tctttctcga ataatgtctc catcattttt
       61 cttccagctg ctttcaaacc ttcttcatct aaggtatctg ttttgggatc gtacaagccc
      121 attttttcaa gatacgaatt gtcgtgagag ataacatctt gataaatgat tgagcctttt
      181 tgttgtcctc tttggaagat ttgtttgatc tccttcagat cacttccttt cacttcgtct
      241 gaagccatag aaaaaactcc aggaagattt cctgaagctt ttttatgtgg ctctgtttga
      301 tattttttca ttgcttggtt caaccgttgg tattcttttt gttccctttg cgtcagttct
      361 ttcttttgtt ctaacgcata gtgacgggtc atataagaaa gatatttggt aaaatcttgt
      421 tcctctaact tgtcaaaatt gattccatgt tccatcaacg attgagcttc attc
//
LOCUS       DASHFF010000214          466 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.231, whole genome shotgun sequence.
ACCESSION   DASHFF010000214 DASHFF010000000
VERSION     DASHFF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.231"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>466
                     /locus_tag="U2I75_002960"
     CDS             <1..>466
                     /locus_tag="U2I75_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1697991.1"
                     /translation="TVIHFFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVN
                     AFKVRTLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTEST
                     YVWNELLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          151 a           94 c           89 g          132 t
ORIGIN      
        1 agaccgtcat tcacttcttc cgaaaaggta ttaccatgtc ggaaatcgca gacttgattg
       61 agaaaatgta tgggcatcac tacacgcccc aaaccatgtc caacatgact aaagtgctga
      121 ctgaagaagt aaatgcattt aaagttagaa ctctaaatga taagtatgtc gctattttca
      181 tggatgctac ttatattcca ttaaaacggc aaaccgtctc caaagaagca atctatattg
      241 ccattggtat acgagaagac ggcactaaag aagtactaag ttatgcgatt gctccgactg
      301 aatcgaccta cgtttggaat gagttgttac aggacattta ctccagaggg gttcatgatg
      361 tcttactgtt tattaccgat ggcttaaaag gcatgaaaga tacgattcac cagatttatc
      421 ctaaagctaa ataccaacat tgctgtgttc acgtttcccg taatat
//
LOCUS       DASHFF010000215          457 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.232, whole genome shotgun sequence.
ACCESSION   DASHFF010000215 DASHFF010000000
VERSION     DASHFF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.232"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..411
                     /locus_tag="U2I75_002961"
     CDS             <1..411
                     /locus_tag="U2I75_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1697992.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
BASE COUNT          144 a           76 c           87 g          150 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagat
//
LOCUS       DASHFF010000216          451 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.233, whole genome shotgun sequence.
ACCESSION   DASHFF010000216 DASHFF010000000
VERSION     DASHFF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.233"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(36..272)
                     /locus_tag="U2I75_002962"
                     /pseudo
     CDS             complement(36..272)
                     /locus_tag="U2I75_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          149 a           74 c           77 g          151 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaataagg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaataac gccgtttgta taattaagcg agacaaataa
      301 aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa ggagagtgaa
      361 cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa gcatattttc
      421 atcaagaaac tccggttgct atcgttgcga a
//
LOCUS       DASHFF010000217          436 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.234, whole genome shotgun sequence.
ACCESSION   DASHFF010000217 DASHFF010000000
VERSION     DASHFF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.234"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(178..>436)
                     /locus_tag="U2I75_002963"
     CDS             complement(178..>436)
                     /locus_tag="U2I75_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1697993.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          151 a           58 c           76 g          151 t
ORIGIN      
        1 atcgaaaatg gtagaaaaga aatttataaa aggacttata aagattgatt atataatata
       61 tgaaaagcta ttataaaaga cattagtatt aaatagttta aaaaaatgaa aaataataaa
      121 ggaagtgagt caattccaga ctcctgtgta aaatgctata caatgttttt accatttcta
      181 cttatcaaaa ttgatgtatt ttcttgaaga ataaatccat tcatcatgta ggtccataag
      241 aacggctcca attaagcgat tggctgatgt ttgattgggg aagatgcgaa taatcttttc
      301 tcttctgcgt acttcttgat tcagtcgttc aattagattg gtactcttta gtcgattgtg
      361 ggaatttcct tgtacggtat attgaaaggc gtcttcgaat ccatcatcca atgatgcgca
      421 agcttttgaa tatttt
//
LOCUS       DASHFF010000218          419 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.235, whole genome shotgun sequence.
ACCESSION   DASHFF010000218 DASHFF010000000
VERSION     DASHFF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.235"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>419
                     /locus_tag="U2I75_002964"
     CDS             <1..>419
                     /locus_tag="U2I75_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697994.1"
                     /translation="TFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIYNYHG
                     WEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEEGGAS
                     SLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          164 a           55 c           82 g          118 t
ORIGIN      
        1 tacatttgtt tttaagaaaa aagtagtttc agactattta aataacgaag gcggctacaa
       61 atatcttgca cataaatatc aaataaatcg tacactggtt agacattggg taaggattta
      121 taactatcat ggttgggaag gcttggttgg aggtggcaaa agctacacta caaaatttaa
      181 acttgatgtt atagaatata tggaaacaaa tggtctttct atccaagaaa ctgctaaaaa
      241 atttaatatc ggttcaaata gaactctaag taaatggata gagcaatatg aagaaggcgg
      301 tgcttcttca cttgagagcc aaaaaagggg cagaaaaatt agtatgaatt ccaagctaaa
      361 cattcctaaa aaacttaaag atgagtctct tgaagaagaa gttattcgtt taagagcag
//
LOCUS       DASHFF010000219          415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.236, whole genome shotgun sequence.
ACCESSION   DASHFF010000219 DASHFF010000000
VERSION     DASHFF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.236"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..390
                     /locus_tag="U2I75_002965"
     CDS             <1..390
                     /locus_tag="U2I75_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1697995.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          115 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatt
//
LOCUS       DASHFF010000220          415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.237, whole genome shotgun sequence.
ACCESSION   DASHFF010000220 DASHFF010000000
VERSION     DASHFF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.237"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..339)
                     /locus_tag="U2I75_002966"
     CDS             complement(<1..339)
                     /locus_tag="U2I75_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1697996.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DASHFF010000221          402 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.238, whole genome shotgun sequence.
ACCESSION   DASHFF010000221 DASHFF010000000
VERSION     DASHFF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.238"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>402)
                     /locus_tag="U2I75_002967"
     rRNA            complement(<1..>402)
                     /locus_tag="U2I75_002967"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           79 a          126 c           93 g          104 t
ORIGIN      
        1 atgaccctcc ccggttgagc cgggggcttt cacatcagac ttaagaaacc gcctgcgctc
       61 gctttacgcc caataaatcc ggacaacgct tgccacctac gtattaccgc ggctgctggc
      121 acgtagttag ccgtggcttt ctggttagat accgtcaagg gatgaacagt tactctcatc
      181 cttgttcttc tctaacaaca gagttttacg atccgaaaac cttcttcact cacgcggcgt
      241 tgctcggtca gactttcgtc cattgccgaa gattccctac tgctgcctcc cgtaggagtt
      301 tgggccgtgt ctcagtccca atgtggccga tcaccctctc aggtcggcta tgcatcgtgg
      361 ccttggtgag ccgttacctc accaactagc taatgcaccg cg
//
LOCUS       DASHFF010000222          391 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.239, whole genome shotgun sequence.
ACCESSION   DASHFF010000222 DASHFF010000000
VERSION     DASHFF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.239"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>391
                     /locus_tag="U2I75_002968"
     CDS             <1..>391
                     /locus_tag="U2I75_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEN1697997.1"
                     /translation="QIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKY
                     LNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          146 a           70 c           72 g          103 t
ORIGIN      
        1 atcaaatttg gaaaaagaaa aataaaaaat ccttttattc atggaaaatg gatgaaacgt
       61 acatcaaaat taaaggaaaa tggcattatt tgtatcgagc catcgatgca gatggtttaa
      121 ccttggatat ttggttacgt aaaaaacggg acacacaagc agcctatgct tttcttaagc
      181 ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac agataaagcc ccctctatta
      241 caagtgcctt taagaaacta aaagaatacg gcttttatca agggacagaa catcgtacca
      301 ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga cgcaataaat
      361 tctatcgaag tttacgcact gcctctacca c
//
LOCUS       DASHFF010000223          381 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.240, whole genome shotgun sequence.
ACCESSION   DASHFF010000223 DASHFF010000000
VERSION     DASHFF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.240"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            139..>381
                     /locus_tag="U2I75_002969"
     CDS             139..>381
                     /locus_tag="U2I75_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1697998.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
BASE COUNT          131 a           44 c           71 g          135 t
ORIGIN      
        1 ctatctttta ttttaaaata tgggatatta tgataaatta actatggatg atatttgaaa
       61 aaaacatact gtatatttga tttaattttt atttttaagc tcatatcgta agaatgggac
      121 ttgtagtctc acttctttat ggaggtgata attatgaacg ttggttttat tggagccggc
      181 aaggtaggtt gttcattcgg gaagtatttc caggaacata aaatacaagt aacaggattt
      241 tatagtaaaa gcgaagactc gtcattagca gcttcgaatt ttacatctag taaacaatat
      301 ttaaacttaa gagaattagt agatgaaaat gatactattt tcattacgac tcctgatggt
      361 caaattcagg aggtatggca a
//
LOCUS       DASHFF010000224          367 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.241, whole genome shotgun sequence.
ACCESSION   DASHFF010000224 DASHFF010000000
VERSION     DASHFF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.241"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            71..>367
                     /locus_tag="U2I75_002970"
     CDS             71..>367
                     /locus_tag="U2I75_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1697999.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEE"
BASE COUNT          102 a           68 c           73 g          124 t
ORIGIN      
        1 tttatggatg atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg
       61 agtgatagct atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac
      121 gattgatgat ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt
      181 tgaatttatt tatccaatcg tcgaagccac ttactccgat ttaggtcgcc caagtatcga
      241 tccagtgatt ctgatcaaat tggtgtttat tcagtattta ttcggtatcc gttccatgcg
      301 acaaaccatt aaggaagtcg ataccaatgt cgcttaccgc tggtttttag gctactcctt
      361 cgaggag
//
LOCUS       DASHFF010000225          342 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.242, whole genome shotgun sequence.
ACCESSION   DASHFF010000225 DASHFF010000000
VERSION     DASHFF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.242"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..270
                     /locus_tag="U2I75_002971"
                     /pseudo
     CDS             1..270
                     /locus_tag="U2I75_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          124 a           46 c           64 g          108 t
ORIGIN      
        1 aaatctatat tttgatatac aaattacagc ttcagatact aagttgcata aaccttttcc
       61 ggaccccata caaaaggcaa ttgatgaatt gggcataaaa aataagaaac gatttatatt
      121 ggtgattcat tatatgacat gcaaagtgcg aaagcttgtg ggatagcatt tggtttagct
      181 aaatggggag ccaaaaatat agaaaatttt caaagtgtag atataattat tgattctcct
      241 caagaaatac ttgatttgtt agaaaaatag acaaagagcg tgagataagg atagggtctc
      301 acgctctttg tttttcgcca tttaaagatt gaaatttttg cc
//
LOCUS       DASHFF010000226          329 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.denovo.243, whole genome shotgun sequence.
ACCESSION   DASHFF010000226 DASHFF010000000
VERSION     DASHFF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.denovo.243"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>329
                     /locus_tag="U2I75_002972"
     CDS             <1..>329
                     /locus_tag="U2I75_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1698000.1"
                     /translation="IKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIA
                     KVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGD
                     KGYISHK"
BASE COUNT          120 a           58 c           54 g           97 t
ORIGIN      
        1 tatcaagatt attcgatact ttttcatcaa gaagctcacc aaaggtgaac tagtcggaat
       61 tatagatagt tttcctagtc cattatgtaa accagttaga aatagacaag caaaactatt
      121 gaatcaaata gccaaagttg gctataactc aacaaaaaaa tcttattttt atggtcttaa
      181 aattcatatg atcgttacta agactggctt tccgattact tactcaatca cgaatccagg
      241 tgtccatgac gtgaaagtgt tagagacttt atcggaagaa gcgaatcttc ccaatatcct
      301 aggggacaaa ggatatatta gccataaaa
//
LOCUS       DASHFF010000227         7777 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.098, whole genome shotgun sequence.
ACCESSION   DASHFF010000227 DASHFF010000000
VERSION     DASHFF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.098"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            242..1210
                     /locus_tag="U2I75_002973"
     CDS             242..1210
                     /locus_tag="U2I75_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN1698001.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1203..2234
                     /gene="vanA"
                     /locus_tag="U2I75_002974"
     CDS             1203..2234
                     /gene="vanA"
                     /locus_tag="U2I75_002974"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN1698002.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2240..2848
                     /locus_tag="U2I75_002975"
     CDS             2240..2848
                     /locus_tag="U2I75_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN1698003.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3276..4187
                     /gene="vanY"
                     /locus_tag="U2I75_002976"
     CDS             3276..4187
                     /gene="vanY"
                     /locus_tag="U2I75_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEN1698004.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4340..4825
                     /gene="vanZ"
                     /locus_tag="U2I75_002977"
     CDS             4340..4825
                     /gene="vanZ"
                     /locus_tag="U2I75_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN1698005.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5112..5441)
                     /locus_tag="U2I75_002978"
     CDS             complement(5112..5441)
                     /locus_tag="U2I75_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1698006.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5746..6075
                     /locus_tag="U2I75_002979"
     CDS             5746..6075
                     /locus_tag="U2I75_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1698007.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6065..6352
                     /locus_tag="U2I75_002980"
     CDS             6065..6352
                     /locus_tag="U2I75_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1698008.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(6631..7194)
                     /locus_tag="U2I75_002981"
     CDS             complement(6631..7194)
                     /locus_tag="U2I75_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1698009.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2566 a         1297 c         1545 g         2369 t
ORIGIN      
        1 ctggtgtcat ccaccaacat caaaaagtat agagccataa ttttttagga aaatctcaag
       61 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
      121 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
      181 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
      241 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
      301 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
      361 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
      421 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
      481 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
      541 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
      601 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
      661 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
      721 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
      781 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
      841 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
      901 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
      961 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     1021 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     1081 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     1141 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     1201 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     1261 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     1321 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     1381 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     1441 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     1501 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     1561 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     1621 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     1681 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     1741 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     1801 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     1861 tgggaaacag tgccgcgtta gctgttggcg aggtggacca aatcaggctg cagtacggaa
     1921 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     1981 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     2041 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     2101 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     2161 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     2221 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     2281 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     2341 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     2401 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaatcg
     2461 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     2521 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     2581 aagccatagc cgcggcagtg ccattgatct tacgctttat cgattagaca cgggtgagct
     2641 tgtaccaatg gggagccgat ttgattttat ggatgaacgc tctcatcatg cggcaaatgg
     2701 aatatcatgc aatgaagcgc aaaatcgcag acgtttgcgc tccatcatgg aaaacagtgg
     2761 gtttgaagca tatagcctcg aatggtggca ctatgtatta agagacgaac cataccccaa
     2821 tagctatttt gatttccccg ttaaataaac ttttaaccgt tgcacggaca aactatataa
     2881 gctaactctt tcggcaggaa acccgacgta tgtaactggt tcttagggaa tttatatata
     2941 gtagatagta ttgaagatgt aaggcagagc gatattgcgg tcattatctg cgtgcgctgc
     3001 ggcaagatag cctgataata agactgatcg catagagggg tggtatttca caccgcccat
     3061 tgtcaacagg cagttcagcc tcgttaaatt cagcatgggt atcacttatg aaaattcatc
     3121 tacattggtg ataatagtaa atccagtagg gcgaaataat tgactgtaat ttacggggca
     3181 aaacggcaca atctcaaacg agattgtgcc gtttaagggg aagattctag aaatatttca
     3241 tacttccaac tatatagtta aggaggagac tgaaaatgaa gaagttgttt tttttattgt
     3301 tattgttatt cttaatatac ttaggttatg actacgttaa tgaagcactg ttttctcagg
     3361 aaaaagtcga atttcaaaat tatgatcaaa atcccaaaga acatttagaa aatagtggga
     3421 cttctgaaaa tacccaagag aaaacaatta cagaagaaca ggtttatcaa ggaaatctgc
     3481 tattaatcaa tagtaaatat cctgttcgcc aagaaagtgt gaagtcagat atcgtgaatt
     3541 tatctaaaca tgacgaatta ataaatggat acgggttgct tgatagtaat atttatatgt
     3601 caaaagaaat agcacaaaaa ttttcagaga tggtcaatga tgctgtaaag ggtggcgtta
     3661 gtcattttat tattaatagt ggctatcgag actttgatga gcaaagtgtg ctttaccaag
     3721 aaatgggggc tgagtatgcc ttaccagcag gttatagtga gcataattca ggtttatcac
     3781 tagatgtagg atcaagcttg acgaaaatgg aacgagcccc tgaaggaaag tggatagaag
     3841 aaaatgcttg gaaatacggg ttcattttac gttatccaga ggacaaaaca gagttaacag
     3901 gaattcaata tgaactatgg catattcgct atgttggttt accacatagt gcgattatga
     3961 aagaaaagaa tttcgttctc gaggaatata tggattacct aaaagaagaa aaaaccattt
     4021 ctgttagtgt aaatggggaa aaatatgaga tcttttatta tcctgttact aaaaatacca
     4081 ccattcatgt gccgactaat cttcgttatg agatatcagg aaacaatata gacggtgtaa
     4141 ttgtgacagt gtttcccgga tcaacacata ctaattcaag gaggtaagga tggcggaatg
     4201 aaaccaacga aattaatgaa cagcattatt gtactagcac ttttggggta acgttagctt
     4261 tttaatttaa aacccacgtt aactaggaca ttgctatact aatgatacaa cttaaacaaa
     4321 agaattagag gaaattatat tgggaaaaat attatctaga ggattgctag ctttatattt
     4381 agtgacacta atctggttag tgttattcaa attacaatac aatattttat cagtatttaa
     4441 ttatcatcaa agaagtctta acttgactcc atttactgct actgggaatt tcagagagat
     4501 gatagataat gttataatct ttattccatt tggcttgctt ttgaatgtca attttaaaga
     4561 aatcggattt ttacctaagt ttgcttttgt actggtttta agtcttactt ttgaaataat
     4621 tcaatttatc ttcgctattg gagcgacaga cataacagat gtaattacaa atactgttgg
     4681 aggctttctt ggactgaaat tatatggttt aagcaataag catatgaatc aaaaaaaatt
     4741 agacagagtt attatttttg taggtatact tttgctcgta ttattgctcg tttaccgtac
     4801 ccatttaaga ataaattacg tgtaagatgt ctaaatcaag caatctgatc tttcatacac
     4861 ataaagatat tgaatgaatt ggattagatg gaaaacggga tgtggggaaa ctcgcccgta
     4921 ggtgtgaagt gaggggaaaa ccggtgataa agtaaaaagc ttacctaaca ctatagtaac
     4981 aaagaaagcc caattatcaa ttttagtgct gaggaattgg tctctttaat aaatttcctt
     5041 aacgttgtaa atccgcattt tcctgacggt acccccaaac taagatttca tagatagatt
     5101 ttctgataac tttaaaaaac acgaatagcg gaaaggatgt cttcattcgc taaagagtaa
     5161 taaactactt ttccatcttg acgtgatttt gccattccat gtttttttaa aaaacgtaaa
     5221 tggtgtgacg ttgtagcgac actagcagta agtatttctg ctaaatcaca gacgcacatt
     5281 tccttatagg tttctaaagc atagaaaatt ttaattctag aaaaatcgct gaaacatttc
     5341 cctaacacaa gtaagtttga aaaatctttt tgatccaatt tattttttat tccatttact
     5401 ttttctttat gtacgacagt cacttggcaa acttttttca ttcatctcac ctcacattca
     5461 aacgattatt tgaatgaagt atacctattt gatcactctt ttgtcaatct accttaattc
     5521 caatacacta ttataagcaa tttcatgtat tgaagtaaac ttacttgcaa gtgcgttttt
     5581 gattccaagt taaaaacata ttattattct acataatttc ctaatcaaat aaaactcctt
     5641 tgaaaattgt tctaaaggag ttttattatt ttgtatttct actaaaaata gcgtacaatg
     5701 taattactaa gtaattacga aatgtaaaat aatggaggaa ataatatgat tacaaaaaca
     5761 agaaaacaag gaaattctat tatgttaacg gtccctaaag aattcgatgt gcccaatggt
     5821 gttgaagtag aagctaagct tgtagagaat ggtatccttt atgagtttgt agagcctaaa
     5881 aaagaatttt ttgattttag tgaagatatt ttggctgata ttctttcaga aggttatagc
     5941 aaacaagaga tcttaaaaga atttagaaat agaaaaaatg cgttaacttc tgcatttaaa
     6001 tctattgctg aagatactgt tgcaaattct aaaccgatga caaaagagga gttggcagca
     6061 gaaattggct tatgagattc taccttctaa acacgtaata aagtatctaa aaaaattaaa
     6121 agagaaaaca ttaaaagaac aatttttaac gataatttat gatgagatag cagttcgtcc
     6181 tcattcggga gaacaaaaaa cgggtgattt atcaggaatt tgggctattg gattcaagta
     6241 tgcaggtacg acatatcgcg ttgcatatga aattaaagat aatgcagtga tacctaactt
     6301 actatgtgga acgcatgaaa atttttatga acaattaaaa aagattaggt aagaacccaa
     6361 tgtttcaact tgagaggata gacgacactt taaaagataa tttttaacag ctgagagttg
     6421 taatacccta taattttaaa ccactaacct atatatttat gaaaaataga aaaaattaaa
     6481 acgcatttta agcctctttc aaattaaaag cttcatcctt atgcacaaag agggcttaaa
     6541 atgcgttatt ttatattgtt ttttagtaat aataaaaaaa tcactctaat tctcccataa
     6601 atattataga ttcctaaaga ttaaaataga ttattcccta ttgtctttta agtagcggta
     6661 gagtgttgcc ttgctaatac cagtcatttc cgtaatttct tttacgctat aaacctgact
     6721 tttatacaat tttactgctc gatcaacatc tttctggttc actcgtggac ggccaccttt
     6781 acgacctctc gcacgtgcac ttttcaaacc ttctttggta cgttcaacaa ttaaatcacg
     6841 ttcgaactga ctaaatgctt ggaagacagt catcattaag cgtccgtgag gcgtgctggt
     6901 atcgaagttt tctttcaagc tcaccagctt aacatggtgt tcttcaaaat aggtaacaag
     6961 gtcaatcaaa tcctttgtac tacgacccaa tcgagaaaaa ctttctacaa ttaccgtatc
     7021 tcccggacga attttatctt ttaaacgatt tagttgggga cgaaccgctt tggttcccgt
     7081 cattttctca gttaggattt cattacaatt ttgttcattg agtaagtcta attgacgatt
     7141 tagttcttgt gaacgtgtac tcactcgggc ataaccataa atgtatccac tcatgctttt
     7201 cttcctttcg tactttaatt ctggcttgat tgtatcataa aagggagtta tagatacata
     7261 gttaatgcaa caagttatga tacaaaatat gacctctttt atttaataaa agaggtcatt
     7321 tcattttgtc tcataatcga ttgtttttga tacgagtgtg tatacgaaat aaacaaaaaa
     7381 atcttaagta aatttctaga tacctaattt aatcctaact agtgtatcct aaagttaata
     7441 acgagtttat cacaatgaaa attataaaat gctcccatat tagatataac atgcaatatt
     7501 tgaattagaa ctacaaaatt tattcgtttt ataaagtaaa taatgttgag atactaagga
     7561 atagaatatt ataaggaatt gagctgaatg tattggataa aaaaagaata actttattag
     7621 agatggacta taaaaatgct cgtaattttc ttctgaagtc tgaaagctat ttgaatgctc
     7681 agctaccaga atatattagt tttgacgaaa ctttaaaatc tgctaaaaaa gttttaactt
     7741 cgccgtttgt ataattaagc gagacaaata aaaaagc
//
LOCUS       DASHFF010000228         7476 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.102, whole genome shotgun sequence.
ACCESSION   DASHFF010000228 DASHFF010000000
VERSION     DASHFF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.102"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            107..190
                     /locus_tag="U2I75_002982"
     CDS             107..190
                     /locus_tag="U2I75_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN1698010.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            315..1052
                     /gene="erm(B)"
                     /locus_tag="U2I75_002983"
     CDS             315..1052
                     /gene="erm(B)"
                     /locus_tag="U2I75_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1698011.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1222..1482
                     /locus_tag="U2I75_002984"
                     /pseudo
     CDS             1222..1482
                     /locus_tag="U2I75_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1645..2310
                     /locus_tag="U2I75_002985"
                     /pseudo
     CDS             1645..2310
                     /locus_tag="U2I75_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2346..2591
                     /locus_tag="U2I75_002986"
     CDS             2346..2591
                     /locus_tag="U2I75_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1698012.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2694..3563
                     /locus_tag="U2I75_002987"
     CDS             2694..3563
                     /locus_tag="U2I75_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN1698013.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3544..4278
                     /locus_tag="U2I75_002988"
     CDS             3544..4278
                     /locus_tag="U2I75_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1698014.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4311..5219
                     /locus_tag="U2I75_002989"
     CDS             4311..5219
                     /locus_tag="U2I75_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN1698015.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5216..5758
                     /gene="sat4"
                     /locus_tag="U2I75_002990"
     CDS             5216..5758
                     /gene="sat4"
                     /locus_tag="U2I75_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEN1698016.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5851..6645
                     /locus_tag="U2I75_002991"
                     /note="aph(3')-IIIa"
     CDS             5851..6645
                     /locus_tag="U2I75_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEN1698017.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6921..7463
                     /locus_tag="U2I75_002992"
     CDS             6921..7463
                     /locus_tag="U2I75_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1698018.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2622 a         1129 c         1566 g         2159 t
ORIGIN      
        1 atttgatagt agatttaaaa ctttgcaaca gaaccatttt gtgtataata ggaattgaag
       61 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
      121 aatgcgtaat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
      181 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
      241 gataactaaa attacaaaca aatcgtttaa cttctgtatt tatttacaga tgtaataact
      301 tcaggagtga ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
      361 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
      421 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
      481 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaacattc
      541 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
      601 aaattgttgg gagtattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
      661 aaagccatgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
      721 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
      781 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
      841 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
      901 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
      961 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta
     1021 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttt taaatttgga
     1081 aagttacacg ttactaaagg gaatggagat aaattattag atatactact gacagcttcc
     1141 aagaagctaa agaggtccct agcgcctacg gggaatttgt atcgataagg ggtacaaatt
     1201 cccactaagc gctcgggacc ccttgtagga aaatgtccta agtgtggcaa caatattgta
     1261 ttaaaaaaat cgttttatgg ttgttcaaat tatcctgaat gtaagtttac tttagctgaa
     1321 cattttagaa agaaaaaact aaccaaaacg aatgtaaaag aattactgga gggaaaagaa
     1381 accctggtaa aaggaatcaa aaacaaagag aaaaagccct acaatgccgt tgtaaaaatt
     1441 ggggaaaagg gatatattga ttttatctct ttttcaaaat aaacatgggg ctgacccaaa
     1501 agtagctcct atatgaaaaa tagaggtctt tccatttaga tagtagtgga aagacctcta
     1561 tttatctttt gaaagtgaat acaaaaataa agcacactct gctataatat aggtgattaa
     1621 tccatttgag agaggtgctt tatcatgtat caaaattata ccataaacca actttgtttg
     1681 cctatagatc ttgaaattaa attagaagaa aatgattttg ctcatgccat tgtgcagttt
     1741 gttgactcta ttcccgatga agtctttctt ccttattatc aatctatggg tagaccccaa
     1801 tatcatcctc gtatgctact ttccattatt ctttgtgcct atattcaagg ggtttattcg
     1861 ggccgccaaa tacaaaatat gctgatagat agtattcgga tgcgctactt atctcaagag
     1921 caattcccta actttcgtac tattaatcgt tttcgcgttc atccgattat gaacgatatt
     1981 ctagatcatt catttgttca atttcgtgag ctactggttc aaagtggctt aatcagtggc
     2041 agtgctctat acattgatgg tacaaaaatt gaagcggatg cgaataaata ctctttcgtt
     2101 tggaaaaaaa gtattttaaa gtataaggat agccttgatc aaaaagccct tgaaaactac
     2161 caacaaatgg tggaaacaaa aatcttacct gagttaatag atgatttaaa ggatgaactt
     2221 tctatagaag agattgaaag gattcgtcaa tctttggaag aaaaggaaaa tcaattaatt
     2281 gaagcgattg accaaaccga aacagtagaa gggggctgac ccattattaa taaggagtgg
     2341 acaacatgat tgatgtaaat gagctattgg aagaagctat tagagaaact gaaaatctaa
     2401 atgatggcga agtttttctt gttaaagatt tattcaaggg atatgtgggg aataggatac
     2461 ccagaaagga tcgacttctg cttggaacgt tatttttaaa ccatgttagc aaaatgaatg
     2521 gtaatttaaa ggctattgaa aagacttcat ctaatcagca gaggtataaa aagacaattg
     2581 ataagtttta actatctttt gtgaaattaa aaaaattata aatatgttga gtagaagcaa
     2641 aagatttggc ttggaatttt tacagcagtg aatcgataaa aggagcatgc ttgatggtag
     2701 ataatattat taaatcagta gcagagaaat tatcctctct gtcttatata gaaggtattg
     2761 ttttaggtgg ttcacgtgca aggggcaccc atacagagga ttcggatata gatatcggca
     2821 tctattacaa ttcagaatca tttgacataa atactattaa tcaattcgct acaaagctgg
     2881 atgatgagca tagaaataac cttgttgtac ctcccggagc atggggtgat tggattaatg
     2941 gcggcggatg gttagtcata aacgggtatc atgtggattt aattttacgt gatattaaac
     3001 gtgtggaaca aataatgaaa gatacagagc acggaattgt tactgccaat tatcagactg
     3061 ggcatcccca tggttatatt agtgcaatgt atcgaggaga attagcgatt agcaaaatac
     3121 tatatgctaa gaatgaaagc ttatgcgaat taaaaaaaca ggcagaaact tatcccaatg
     3181 ctttgcagaa aagtttagtt aactttttta tgtttgaagc agggttctct ttaatgtttg
     3241 taaaagcaaa ttcgggaaca gacgataaat attatattgc gggtcatgtt tttcgtatag
     3301 tttcatgttt aaatcaagtg ttatttgcat gtaataatgc ttattgtatc aacgaaaaga
     3361 aagctataaa actgcttgaa acttttgaac ataaacctga aaaatatacc gagaaggtaa
     3421 atcatatttt tgaagtactc ggtatctcac tttttgaatg ctacgacatg accgagaagc
     3481 tttataatga agtgaatgaa attgtatcgg agataaataa ctttttaaac gaggagagtt
     3541 cagatgaaag aaaacaaata tgatgataat atattttttc aaaaatacag tcaaatgagt
     3601 cgctcgcaga aaggactggc tggtgcggga gaatgggaga ctttgaaaaa gatgctacct
     3661 gattttaagg gtaagcgtgt gcttgattta ggatgcggct atggatggca ctgtatatat
     3721 gcgatggaaa acggtgcttc ctctgtagta ggtgttgata tttctcataa aatgctcgaa
     3781 gtagcaaaag gaaaaaccca ttttccacag attgaatatg aatgctgtgc catagaagat
     3841 gtggatttcc cagaggagag ctttgatgta atactaagtt cgcttgcgtt tcattatgta
     3901 gcagactatg agaatttaat aaaaaagata tataggatgc tgaaggctgg tggcaattta
     3961 gtttttacag ttgaacatcc tgtttttact gctcatggaa cacaagactg gtattataac
     4021 gaaaaaggag aaatactgca tttcccggtg gacaattatt attatgaggg caaacggaca
     4081 gctatgtttt tggaagaaaa ggttacaaaa tatcatagaa cactgaccac atatctaaat
     4141 acactgcttt caaatagttt tataataaat cagattgtgg agccacagcc gccagagaac
     4201 atgatggata ttccggggat ggcggatgaa atgcgacgcc caatgatgct gattgtatcg
     4261 gcaaaaaaga agatgtaata atatagaaaa aataaacgag gagtatgtaa atgagatcag
     4321 aaaaagaaat gatggattta gtactttctt tagcagaaca ggatgaacgt attcgaattg
     4381 tgacccttga ggggtcacgc gcaaatatta atatacctaa agatgaattt caggattatg
     4441 atattacata ttttgtaagt gatatagaac cgtttatatc taatgatgac tggcttaatc
     4501 aatttgggaa tataataatg atgcaaaagc cggaggatat ggaattattc ccacctgaag
     4561 aaaagggatt ttcctatctt atgctatttg atgattacaa taaaattgat cttaccttat
     4621 tgcccttgga agagttagat aattacctaa agggcgataa attaataaag gttctaattg
     4681 ataaagattg tagaattaaa agggacatag ttccgactga tatagattat catgtaagaa
     4741 agccaagcgc aagggagtat gatgattgct gcaatgaatt ttggaatgta acaccttatg
     4801 ttattaaagg attgtgccgt aaggaaattt tatttgctat tgatcatttt aatcagattg
     4861 ttcgccatga gctgctgaga atgatatcat ggaaggtcgg catcgaaaca ggctttaaat
     4921 taagtgtagg caagaactat aagtttattg aaaggtatat atccgaggat ttgtgggaga
     4981 aacttttgtc cacctaccgg atggattcct atgaaaacat atgggaagca ttatttctat
     5041 gccatcaatt gttcagggcg gtatccggtg aggtggcgga aaggcttcat tatgcctatc
     5101 cggagtatga taggaatata acaaaatata ccagggacat gtataaaaaa tacactggta
     5161 aaaccggctg cctggatagc acatatgccg ctgatataga agagaggcgg gaacagtgat
     5221 tacagaaatg aaagcagggc acctgaaaga tatcgataaa cccagcgaac catttgaggt
     5281 gataggtaag attataccga ggtatgaaaa cgagaattgg acctttacag aattactcta
     5341 tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat gaagaggatg aggaggcaga
     5401 ttgccttgaa tatattgaca atactgataa gataatatat ctttactacc aagacgataa
     5461 atgcgtcgga aaagttaaac tgcgaaaaaa ttggaaccgg tacgcttata tagaagatat
     5521 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
     5581 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
     5641 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
     5701 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
     5761 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
     5821 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
     5881 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
     5941 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
     6001 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
     6061 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
     6121 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
     6181 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
     6241 ccctatacga atagcttaga cagccgatta gccgaattgg attacttact gaataacgat
     6301 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
     6361 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     6421 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     6481 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     6541 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     6601 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     6661 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     6721 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata gagttctata
     6781 ttagagatgt aaagagtttt ggtgaagagg tttgtgagta tggcttttat aaccagggtg
     6841 caatgagaag cactaacagc agctagctta agaacgcata ggatatactt tttatggaag
     6901 tcacagagga ggaataaaat ttgaacagga ttaacagagt aacctctatt cttattcagc
     6961 tgcaatcaaa aaaaataatt cctgccaaag aaattgcaca gcgatttaat ataagcttaa
     7021 ggacagttta cagagatatc cggacacttg aagaagccgg aataccaatt ggatctgagg
     7081 ccggaaaagg atattttctt gtagagggct tcctacttcc gccggtaatg tttacagcgg
     7141 cggaagtggg tgcattgatt acagcaggga aatttttaaa ttgccatgga gatgaatcat
     7201 ttataaagga ttttgattca gctatgtata aaatcaaatc tattttgaag catggtgaaa
     7261 aaaactatgc acaggagttg gagaacagta ttaacgtgta cagcacatct ggacagaaaa
     7321 atacattggc ggataacgtt attgcagcaa ttcagactgc aatctgcaat aaaagggtaa
     7381 tatcaattca atatcctgca tcaggagggc aagaaccgga aagcagaatg atagagccgg
     7441 ttctgttgca aagttttaaa taaagaataa aatccc
//
LOCUS       DASHFF010000229         3095 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.139, whole genome shotgun sequence.
ACCESSION   DASHFF010000229 DASHFF010000000
VERSION     DASHFF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.139"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            93..590
                     /gene="dfrG"
                     /locus_tag="U2I75_002993"
     CDS             93..590
                     /gene="dfrG"
                     /locus_tag="U2I75_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1698019.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(662..2614)
                     /locus_tag="U2I75_002994"
     CDS             complement(662..2614)
                     /locus_tag="U2I75_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1698020.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2621..2857)
                     /locus_tag="U2I75_002995"
     CDS             complement(2621..2857)
                     /locus_tag="U2I75_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1698021.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          940 a          550 c          514 g         1091 t
ORIGIN      
        1 cttattcaac taacgggcag gataatgaga aaaggttttt tgatagaaat aagaaattag
       61 atggtaaatt agttaaaaag gctgtgttat aaatgaaagt ttctttgatt gctgcgatgg
      121 ataagaatag agtgattggc aaagagaatg acattccttg gaggattccc aaggactggg
      181 aatatgttaa aaatactaca aagggacatc cgataatatt aggtaggaag aaccttgaat
      241 caatcggaag agccttacct gacagaagaa atattattct gacgagagat aaggggttta
      301 cctttaatgg ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta tgtaaaaacg
      361 aagaagaaat ttttattttc ggaggagaac agatttataa tttgtttttc ccttatgttg
      421 agaaaatgta catcacaaaa atacatcatg aattcgaagg agatactttt tttccagaag
      481 tgaattatga ggaatggaat gaggtatttg cccaaaaagg gataaagaat gataaaaatc
      541 cgtataacta ctattttcat gtatatgaaa gaaaaaactt attgagttaa cgggtgcgtt
      601 aattgaagaa gaacagtaaa gataaagcac aagagcttgg gattactcca agctcttttt
      661 gtcatttgaa ataaattata tctttccaat caaattgttt atctgtaatc cctaatcttt
      721 gagcaggtgt caaaaccttt ttatccgctg atttaaaagg gcgacaaaag ttgtagtaag
      781 ttcttagaat tgtaatcgca aattgagcat acttcggatt gaagttagcg taaatatagc
      841 tcttcttatc accacgagct gttgttaatg gtctttcaag agatgatata taacgtctta
      901 tctgttgaat aaagctattg gtcgaatggt cattaacatt taatagcatc tttgcgattt
      961 cgtttggttc cagagcagat aaatccgttc tacaatcaac tgaataaaat cctttatcct
     1021 tagtggctaa tggatgtttg atagggttat cggcatattt gcgatatctt tctccatctt
     1081 tactaatagc ctcttcgtgg aattgatgac ttgttagtaa ttcagttaga taatgagatg
     1141 ccaaagtccg tagaaacttt gttttgaatc ctctgatatc tccccaatcg agcagttctg
     1201 cttttgcttg tccgaattct tttaagcatt gttttctgct tttcgtcttg ttaacttggc
     1261 aaataaaatg atgtgcatcg ctcaacctta attctttcgt gaatactcta taaaaggcgt
     1321 ttctaataga gaagtcatca tcactaatca ttcgccattc gtcggcatta accatttgtt
     1381 taattaacca atagtgagca gttgtagtat aaggagcatc aacatttaaa ccatcgacaa
     1441 atctactcct attagtaatt ttccctaatt cgtgaagata agcattccta ttttcgctat
     1501 cgttagcgga tggttcttgt ggataataag accaatctaa cctatcattt ttacgactaa
     1561 aagtattgag atggtcttct ttgaattttc ttgtatcttc atttaattca tccattgaaa
     1621 tattccaatc ataggcaaca tcagaacgga acacatatct tgataagacc tcagcagata
     1681 caactatata cgtttgcata ttaaggtctt caaaccccgt atatttcttt gaaccttgtc
     1741 ctttcttacg gacgttattt aggtagtagt gcatcttgtc cgtattaagc cacatctcgt
     1801 taaatttctt tgtttgaagg ggttgtgtct catatcgttc taaaaactcc aaacaacgcc
     1861 tatacagcca ctccagctta tgatagtagg ttccaactcc aattcctaaa atatcacaag
     1921 tacgactgac tgaaaccctt ccaacaacca tcttagcgaa cattggaaga atggtgttct
     1981 tttgttggtg ataagtagtg gtttcttctc tttttggtag aacattagtg aacttcttac
     2041 acgccttaca ttgatagcgt tgagatttgc ccctactttt cccccttttg taaaattgct
     2101 tcggctcatt gaatggggtt gattcttctt ccgagcagcc ttctttatga aattgataat
     2161 ctggctcaac atcttggatg ctattgattt ctataaggcg tttaatttct tcaacaacag
     2221 accaattaga taaaggggtt actgtacagt tttgcgatac acctctgcca ataggattgt
     2281 cattgcagta cataccttta tgacctttat ctttggaact accagtcatt ttatatcgac
     2341 ttggtttccc ttttacatct ttgaatttgt gctgttcttt cccaaaatta caacaaaacg
     2401 gattgatgca gtaattatat tgaatgggat attcagaacc gttccattta aaagacgttg
     2461 gcaagaacaa cattttgtta taccttgttg caaatcgttt tggagttaat aaatcatatt
     2521 gtttagccct atcttttaat tcttcgtttg aaatcggaat tttaacatct acaatttcat
     2581 cttccttcgt ggctaatctt ttaagcttgg tcattgtcta tcaacccact ctatctttga
     2641 tacccatttt ttcaacaatc cgcttattac ttcgcttgtt ggcaatccat tcaataaact
     2701 tcttatcgtc taaaatgttc aataagaatt catccactgc ttcgctttca gtacgctctg
     2761 catattcagc gtattgctta actatcaccc ttacttgctc tgatatttcc caatccacag
     2821 acttagcgtt cttatttttt ggctcaagaa attgcatagg aaaatgcacc cctttttgct
     2881 tagtaaattg taaccatttt aacacaccaa tttgcattcc tacaataatt atttcacgtt
     2941 cgcattttta tatacatttt aacttacatt ttgtggtgta aattaacaac aaacgttgat
     3001 ttaacaataa aaaacgagta tcaaattcag ttgcaatttg atactcattt aggtttacat
     3061 tttcactttt tgtactccaa aactgaacta cttaa
//
LOCUS       DASHFF010000230         2973 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.142, whole genome shotgun sequence.
ACCESSION   DASHFF010000230 DASHFF010000000
VERSION     DASHFF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.142"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="U2I75_002996"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="U2I75_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN1698022.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="U2I75_002997"
     CDS             2039..2227
                     /locus_tag="U2I75_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN1698023.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="U2I75_002998"
     CDS             complement(2285..2638)
                     /locus_tag="U2I75_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1698024.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT         1008 a          474 c          564 g          927 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gat
//
LOCUS       DASHFF010000231         2973 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.143, whole genome shotgun sequence.
ACCESSION   DASHFF010000231 DASHFF010000000
VERSION     DASHFF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.143"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="U2I75_002999"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="U2I75_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN1698025.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="U2I75_003000"
     CDS             2039..2227
                     /locus_tag="U2I75_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN1698026.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="U2I75_003001"
     CDS             complement(2285..2638)
                     /locus_tag="U2I75_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1698027.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT         1007 a          474 c          565 g          927 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gat
//
LOCUS       DASHFF010000232         2517 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.147, whole genome shotgun sequence.
ACCESSION   DASHFF010000232 DASHFF010000000
VERSION     DASHFF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.147"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..423
                     /locus_tag="U2I75_003002"
     CDS             <1..423
                     /locus_tag="U2I75_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1698028.1"
                     /translation="PYLIVDLKLCNRTTRITRSTQDLFELIDNIRDKKASLKSLKDTW
                     LDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAG
                     MNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            637..1332
                     /gene="vanR"
                     /locus_tag="U2I75_003003"
     CDS             637..1332
                     /gene="vanR"
                     /locus_tag="U2I75_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN1698029.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1310..2464
                     /locus_tag="U2I75_003004"
     CDS             1310..2464
                     /locus_tag="U2I75_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN1698030.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          866 a          449 c          529 g          673 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ctcgaatcac tcgtagtaca
       61 caagatctat ttgaattaat cgataacata cgagataaaa aggcaagttt aaaatcacta
      121 aaagatacat ggcttgattt atcagaagat aatccataca gccaattctt aattactgta
      181 atggctggtg ttaaccaatt agagcgagat cttattcgga tgagacaacg tgaagggatt
      241 gaattggcta agaaagaagg aaagtttaaa ggtcgattaa agaagtatca taaaaatcac
      301 gcaggaatga attatgcggt aaagctatat aaagaaggaa atatgactgt aaatcaaatt
      361 tgtgaaatta ctaatgtatc tagggcttca ttatacagga aattatcaga agtgaataat
      421 tagccattct gtattccgct aatgggcaat atttttaaag aagaaaagga aactataaaa
      481 tattaacagc ctcctagcga tgccgaaaag ccctttgata aaaaaagaat catcatctta
      541 agaaattctt agtcatttat tatgtaaatg cttataaatt cggccctata atctgataaa
      601 ttattaaggg caaacttatg tgaaagggtg ataactatga gcgataaaat acttattgtg
      661 gatgatgaac atgaaattgc cgatttggtt gaattatact taaaaaacga gaattatacg
      721 gttttcaaat actataccgc caaagaagca ttggaatgta tagacaagtc tgagattgac
      781 cttgccatat tggacatcat gcttcccggc acaagcggcc ttactatctg tcaaaaaata
      841 agggacaagc acacctatcc gattatcatg ctgaccggga aagatacaga ggtagataaa
      901 attacagggt taacaatcgg cgcggatgat tatataacga agccctttcg cccactggag
      961 ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa aattcagtgg agtaaaggag
     1021 cagaacgaaa atgttatcgt ccactccggc cttgtcatta atgttaacac ccatgagtgt
     1081 tatctgaacg agaagcagtt atcccttact cccaccgagt tttcaatact gcgaatcctc
     1141 tgtgaaaaca aggggaatgt ggttagctcc gagctgctat ttcatgagat atggggcgac
     1201 gaatatttca gcaagagcaa caacaccatc accgtgcata tccggcattt gcgcgaaaaa
     1261 atgaacgaca ccattgataa tccgaaatat ataaaaacgg tatggggggt tggttataaa
     1321 attgaaaaat aaaaaaaacg actattccaa actagaacga aaactttaca tgtatatcgt
     1381 tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt cgttcaatga tccgagggaa
     1441 acttggggat tggatcttaa gtattttgga aaacaaatat gacttaaatc acctggacgc
     1501 gatgaaatta tatcaatatt ccatacggaa caatatagat atctttattt atgtggcgat
     1561 tgtcattagt attcttattc tatgtcgcgt catgctttca aaattcgcaa aatactttga
     1621 cgagataaat accggcattg atgtacttat tcagaacgaa gataaacaaa ttgagctttc
     1681 tgcggaaatg gatgttatgg aacaaaagct caacacatta aaacggactc tggaaaagcg
     1741 agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac gttgttatgt acttggcgca
     1801 cgatattaaa acgcccctta catccattat cggttatttg agcctgcttg acgaggctcc
     1861 agacatgccg gtagatcaaa aggcaaagta tgtgcatatc acgttggaca aagcgtatcg
     1921 actcgaacag ctaatcgacg agttttttga gattacacgg tataacctac aaacgataac
     1981 gctaacaaaa acgcacatag acctatacta tatgctggtg cagatgaccg atgaatttta
     2041 tcctcagctt tccgcacatg gaaaacaggc ggttattcac gcccccgagg atctgaccgt
     2101 gtccggcgac cctgataaac tcgcgagagt ctttaacaac attttgaaaa acgccgctgc
     2161 atacagtgag gataacagca tcattgacat taccgcgggc ctctccgggg atgtggtgtc
     2221 aatcgaattc aagaacactg gaagcatccc aaaagataag ctagctgcca tatttgaaaa
     2281 gttctatagg ctggacaatg ctcgttcttc cgatacgggt ggcgcgggac ttggattggc
     2341 gattgcaaaa gaaattattg ttcagcatgg agggcagatt tacgcggaaa gcaatgataa
     2401 ctatacgacg tttagggtag agcttccagc gatgccagac ttggttgata aaaggaggtc
     2461 ctaagagatg tatataattt tggctctttg tcaaatgatg ttggtaaaga gaaatct
//
LOCUS       DASHFF010000233         2248 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.151, whole genome shotgun sequence.
ACCESSION   DASHFF010000233 DASHFF010000000
VERSION     DASHFF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.151"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            107..1585
                     /gene="msr(C)"
                     /locus_tag="U2I75_003005"
     CDS             107..1585
                     /gene="msr(C)"
                     /locus_tag="U2I75_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1698031.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1632..2078
                     /locus_tag="U2I75_003006"
     CDS             1632..2078
                     /locus_tag="U2I75_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1698032.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT          741 a          373 c          481 g          653 t
ORIGIN      
        1 gttcagagta ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc tgctcttttt
       61 tagtatcgat aattgttgtt ttacatgaaa taggagtgta aaacatatgg aaaatttagc
      121 agtaaatata acaaatctac aagtcagctt tggcaaccag ctagaactat ctattgattc
      181 tcttcgtgtc tatcagcaag atcgtatagg gatcatagga gaaaatggtg tcggtaaatc
      241 gactttgctc aaactaatag ccggtgaact ttttcccgat catgggaaaa tccaaacaga
      301 gatcaccttc aactacctgc ctcaattaac ctatctcgct gaagcaaagg acctaaattt
      361 ggaattagcc agtcatttcc agttaagact ggaagaaact tcagagcgga aatggagcgg
      421 aggggaagaa cgaaagatcg agttgatacg tcttctttct tcttatgaac aagggatgct
      481 tctagacgag ccaacaaccc atctagatag aaaaagtatt gatcgactga ttgaagagct
      541 tcgttattat tatggtacgc tggtttttgt tagtcatgat cgctattttc tagatgagct
      601 agcatcgaaa atctgggaag taaaagacgg agaaatccga gagttttcgg ggaattatag
      661 tgcctatctc actcaaaagg aattggagaa aaagactcag ctacgagaag cagagtcgat
      721 catgaaagag aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa
      781 gttggaaaaa gtgtccagta aaaagaaaaa gcaacaaatc agaccggatc ggttgtcttc
      841 ctctaaacaa aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa cattagagag
      901 aagactccaa aaaataggag aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc
      961 agtaccaaaa tctcttgaac tccacagccg ctatcccatc atgggacaaa atgtccaatt
     1021 ggaaagaagt gggagaacat tactggtaaa tggtgatttt cagttttctt taggtaaaaa
     1081 aatcgcgatt gtcggtgaaa atggttcagg taagacaacc ttattagaac atatccgcaa
     1141 acaaggagaa ggaatccttc tctctccgaa agtaagcttt caagtatatc agcaaaaggg
     1201 ttatcaaatg acatctgaag aatccatcat tcgttttgtc atgagacaaa cagagttttc
     1261 agaatcgctt gtccgtagtt tgctgaatca cttagggttt gctcaggaaa ctttgacgaa
     1321 accgttatgt acattaagtg ggggagaagc gacccgtctg acgattgctt tgctttttac
     1381 taagccaagt aatgtgttgc tgttagatga accgactaat tttattgata tggcaacgat
     1441 cgaagcttta gagaagctaa tgcaaatata tccgggaacg attttgttta cttctcatga
     1501 ttcttacttt gtcgagcgta cggctgatga agtttatgaa ataaaagggc agaaaataaa
     1561 aaaagtactt acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg
     1621 agtggtacag attgcctaca aataaaaaag aaggaataat ttttacaaca atgatgtgtt
     1681 ttttgatggt cttggggatg agcgcgtata atttatggct gcatcaagat cttcatttat
     1741 caagtttgtt gattggtcta gtgcctggat tcgtggtggc tttcattttg gatgtcttca
     1801 tcatcggtgt gctggcgaaa aaaattgcct ttaaactacc agtaaatcca gaaagcaagg
     1861 tccaaatgat cttaactatt tcatgcttga tggttttagg aatggtgaca tgtatgtctt
     1921 tattcggtgt gatcatggaa caaggaatac ctgatcaatt attctctgct tatatgaatg
     1981 cttggaaaat gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt
     2041 caagaagtgt gctaaaagca attcaaagta atgaatgatg gaacactgtt atttgaaaaa
     2101 aggccttggc atgctttttg tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc
     2161 gaacgatagg ttttggcctg tcgttcggat cgctttttct tttccaactg tattgctctt
     2221 ttattgaaga taatttgctc ttcaatag
//
LOCUS       DASHFF010000234         2200 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.153, whole genome shotgun sequence.
ACCESSION   DASHFF010000234 DASHFF010000000
VERSION     DASHFF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.153"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            128..1168
                     /locus_tag="U2I75_003007"
     CDS             128..1168
                     /locus_tag="U2I75_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1698033.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1266..1454
                     /locus_tag="U2I75_003008"
     CDS             1266..1454
                     /locus_tag="U2I75_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN1698034.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1512..1865)
                     /locus_tag="U2I75_003009"
     CDS             complement(1512..1865)
                     /locus_tag="U2I75_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1698035.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT          727 a          354 c          393 g          726 t
ORIGIN      
        1 gtacattttg tcattttgta ctccaaaact gaactactta cttgcatata tacgccttta
       61 tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt
      121 tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg
      181 agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa
      241 actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga
      301 accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag
      361 tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt
      421 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
      481 agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac
      541 cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc
      601 gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca
      661 atcatttcaa aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg
      721 ttggaatgtg acggattgta aaatctgttt taagtacggt ttatactata gccctgttag
      781 tactccagca gattttcgga tgcttactcc tattgtactg gagcaagcct ttagaaaagc
      841 tggaacagaa ttgttagagc catatcttag ttttaaagtt tatgcaccac aggaatatct
      901 ttcacgggca tataacgatg ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa
      961 aaataatgag gtcattatta ttggagaaat tcctgctcga tgtattcaag attatcgcaa
     1021 tgatttaact ttttttacaa atgggcttag tgtttgttta gcagagctaa aaggatatca
     1081 ggttaccact ggcgaacctg tttgccagac ccgtcgtcta aatagtcgga tagataaagt
     1141 aagatatatg ttcaataaaa taacttagtg cgttttatgt tgttatataa atatggtttc
     1201 ttattaaata agatgaaata ttctttaata tagatttgaa ttaaagtgga aaggaggaga
     1261 ttgttattat aaactacaag tggatattgt gtcctatttg tggaaataaa acaagactac
     1321 gaatacgagt ggatactata cttaaaaatt tccctttata ctgccccaaa tgtaagaacg
     1381 aaactttaat taatgttcaa aaaatgaata taataacaat caaagagcca gacgccaaga
     1441 cgcagagccg ataatttgag aaatgaaact ctcatcttat cggctctttt tgtttatctg
     1501 aattttactg actagccttc aatatttctt gcttcattga taccttttgc tagtgcttcc
     1561 attaaggata gttctttgtc tgtaaagcta tccatgtatt tttctacctg taaccttcga
     1621 gtgcttttaa tcaaattatc tgtaggtaag aaaaattcat caatagatac atggagtaat
     1681 gatacaagat catacagcac ttgtgtactt gggtgttgtc ctttgttttc tatatttgtt
     1741 aaataacgtg ggtcaatttc aatcattgtt cccacttgtt cacgagtgag accccgtttc
     1801 attcgagcct ctttaatcgc taaaccaacg gctctaaaat cgtatttatc ttctttttta
     1861 cgcatactaa atcacctcta tacattttac tgttcctata taaccataaa caggtataga
     1921 aaaacatgtt atatagttta tgagttccta tagaacactg tggaatttat taatgcgatg
     1981 aatccaattt caaatagaaa ggtcgctgat tttatttacc tatacaactt tataaaattt
     2041 agtcatactc ctagaaagta gataaaattg gcgacttaat ggagggaaaa gtcgccatga
     2101 gaaagataat ctgtcatcta gttctcttaa ttaagttctt atcaaaaaaa cttatccacc
     2161 tgtactggat agatatgacc atcaatgtgg ttttgcagat
//
LOCUS       DASHFF010000235         2028 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.156, whole genome shotgun sequence.
ACCESSION   DASHFF010000235 DASHFF010000000
VERSION     DASHFF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.156"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="U2I75_003010"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="U2I75_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN1698036.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          692 a          318 c          422 g          596 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa acccgtggtg ctagttaatt ttttcctaga ggaaaatg
//
LOCUS       DASHFF010000236         2028 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.157, whole genome shotgun sequence.
ACCESSION   DASHFF010000236 DASHFF010000000
VERSION     DASHFF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.157"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="U2I75_003011"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="U2I75_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN1698037.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          691 a          318 c          423 g          596 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa acccgtggtg ctagttaatt ttttcctaga ggaaaatg
//
LOCUS       DASHFF010000237         1924 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.161, whole genome shotgun sequence.
ACCESSION   DASHFF010000237 DASHFF010000000
VERSION     DASHFF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.161"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            95..1468
                     /gene="tet(L)"
                     /locus_tag="U2I75_003012"
     CDS             95..1468
                     /gene="tet(L)"
                     /locus_tag="U2I75_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HEN1698038.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          558 a          294 c          389 g          683 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtaa gatgaaatat tttttaataa
       61 agatttgaat taaagtgtaa aggaggaatt aattatgaag tgtaatgaat gtaacagggt
      121 taaatacaac caaattttaa tttggctttg cgttttatct ttttttagcg tattaaatga
      181 aatggttttg aacgtctcat tacctgatat tgcaaatgat tttaataaac cacctgcgag
      241 tacaaactgg gtgaacacag cctttatgtt aaccttttcc attggaacag ctgtatatgg
      301 aaagctatct gatcaattag gcatcaaaag gttactccta tttggaatta taataaattg
      361 tttcgggtcg gtaattgggt ttgttggcca ttctttcttt tccttactta ttatggctcg
      421 ttttattcaa ggggctggtg cagctgcatt tccagcactc gtaatggttg tagttgcgcg
      481 ctatattcca aaggaaaata ggggtaaagc atttggtctt attggatcga tagtagccat
      541 gggagaagga gtcggtccag cgattggtgg aatgatagcc cattatattc attggtccta
      601 tcttctactc attcctatga taacaattat cactgttccg tttcttatga aattattaaa
      661 gaaagaagta aggataaaag gtcattttga tatcaaagga attatactaa tgtctgtagg
      721 cattgtattt tttatgttgt ttacaacatc atatagcatt tcttttctta tcgttagcgt
      781 gctgtcattc ctgatatttg taaaacatat caagaaagta acagatcctt ttgttgatcc
      841 cggattaggg aaaaatatac cttttatgat tggagttctt tgtgggggaa ttatatttgg
      901 aacagtagca gggtttgtct ctatggttcc ttatatgatg aaagatgttc accagctaag
      961 tactgccgaa atcggaagtg taattatttt ccctggaaca atgagtgtca ttattttcgg
     1021 ctacattggt gggatacttg ttgatagaag aggtccttta tacgtgttaa acatcggagt
     1081 tacatttctt tctgttagct ttttaactgc ttcctttctt ttagaaacaa catcatggtt
     1141 catgacaatt ataatcgtat ttgttttagg tgggctttcg ttcaccaaaa cagttatatc
     1201 aacaattgtt tcaagtagct tgaaacagca ggaagctggt gctggaatga gtttgcttaa
     1261 ctttaccagc tttttatcag agggaacagg tattgcaatt gtaggtggtt tattatccat
     1321 acccttactt gatcaaaggt tgttacctat ggaagttgat cagtcaactt atctgtatag
     1381 taatttgtta ttactttttt caggaatcat tgtcattagt tggctggtta ccttgaatgt
     1441 atataaacat tctcaaaggg atttctaaat cgttaaggga tcaactttgg gagagagttc
     1501 aaaattgatc ctttttttat aacaggaatt caaatctttt tgttccatta aagggcgcga
     1561 ttgctgaata aaagatacga gagacctctc ttgtatcttt tttattttga gtggttttgt
     1621 ccgttacact agaaaaccga aagacaataa aaattttatt cttgctgagt ctggctttcg
     1681 gtaagctaga caaaacggac aaaataaaaa ttggcaaggg tttaaaggtg gagatttttt
     1741 gagtgatctt ctcaaaaaat actacctgtc ccttgctgat ttttaaacga gcacgagagc
     1801 aaaacccccc tttgctgagg tggcagaggg caggtttttt tgtttctttt ttctcgtaaa
     1861 aaaaagaaag gtcttaaagg ttttatggtt ttggtcggca ctgccgacag cctcgcagag
     1921 caca
//
LOCUS       DASHFF010000238         1891 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.162, whole genome shotgun sequence.
ACCESSION   DASHFF010000238 DASHFF010000000
VERSION     DASHFF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.162"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            269..1771
                     /gene="eat(A)"
                     /locus_tag="U2I75_003013"
     CDS             269..1771
                     /gene="eat(A)"
                     /locus_tag="U2I75_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1698039.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1799..>1891
                     /locus_tag="U2I75_003014"
     CDS             1799..>1891
                     /locus_tag="U2I75_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1698040.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLV"
BASE COUNT          669 a          329 c          411 g          482 t
ORIGIN      
        1 tcccgaagta gttgacaaaa tcattttctt tatgctatcg taatttcaaa tataggacaa
       61 agtgagggat ccgtgcattg aaaaattaag tcgattgatg aaagctgcat tcagactgta
      121 ccttgtggag acaaggaagc agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt
      181 atgggtcttt ttgtgtgtat ccaacccttg tatcctcttt agccagttct gaaaatagga
      241 ctggtctttt tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa atctgacatt
      301 cggctacgac agccaaggca cattattatt tgaacaagca aatctaaatt ttgacacaca
      361 atggaaacta ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatattct
      421 acaaaacaaa ctaccttatc aagggcaagt aatccatcag caagaatttg cctatttccc
      481 gcaacagaca aaagataaag aacgtttaac ctattacgtg ttaaatgata ttacggattt
      541 tgagatatgg gaaatcgaaa gagagctcca attgatgcaa acagatcctg aaatcttatg
      601 gagagaattc agcacactat cggggggaga gaagacaaaa gtcctactgg cacttttatt
      661 tgtggatgac actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg
      721 tagaaaacaa gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca
      781 tgaccgggga tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact
      841 ggaactttat caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt
      901 tgaaatggct caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga aaacagcagc
      961 tgaaaaagcc gaatggtctc gttcccgaga aggagataaa acaaagaaac aagtcggatt
     1021 catcgatact gaatctagac gagtgaataa aggagcagtg ggtgctgatg ctgcacggac
     1081 gatgaaacga tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa
     1141 actattaaaa gatatcgaat atatcgattc tttgacgatg aatagccaag cgtctcacca
     1201 taagcgactt ttaagcgtag aagatcttca attagggtat gaaaatctgt tattcgagcc
     1261 aattcatttt acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg
     1321 aaagtcatcc attatccatt atcttctggg ggcattcaac ggcaaggtta taggagaaaa
     1381 ataccagcca aaacatctga gcattagtta tgcaagccaa aattatgaag acaatcgagg
     1441 aacgttggcg gaatttgcag agaaaaacca agtagactac caagcatttt tgaacaacct
     1501 ccgaaagctt gggatggaaa gagatgtttt tcataacaag atcgagcaga tgagtatggg
     1561 ccaacggaaa aaagtggaat tggctaaatc tttatcacag ccagctgaac tatatacatg
     1621 ggatgaacca ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatctt
     1681 gaacgtgaaa cctgccatgt tactagtgga acatgatcaa accttcctgg ataaagtatc
     1741 tactgagatt atttctcttg agagaatcta acccttgtaa tctcaagaac aacaaggtat
     1801 ggttaagggg aactttgcca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg
     1861 agaaacaact tggaacgcac aaggattagt t
//
LOCUS       DASHFF010000239         1255 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.184, whole genome shotgun sequence.
ACCESSION   DASHFF010000239 DASHFF010000000
VERSION     DASHFF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.184"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            128..1168
                     /locus_tag="U2I75_003015"
     CDS             128..1168
                     /locus_tag="U2I75_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HEN1698041.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
BASE COUNT          411 a          198 c          251 g          395 t
ORIGIN      
        1 gtacattttg tcattttgta ctccaaaact gaactactta cttgcatata tacgccttta
       61 tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt
      121 tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg
      181 agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa
      241 actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga
      301 accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag
      361 tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt
      421 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
      481 agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac
      541 cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc
      601 gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca
      661 atcatttcaa aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg
      721 ttggaatgtg acggattgta aaatctgttt taagtatggc ttatactata gccctgttag
      781 taccccagca gattttcgga tgcttgctcc tattgtattg gaacaagtct taaaaaaagc
      841 tggaacagaa ttgttagagc catatcttag ttttaaaatt tatgcgccac aggaatatct
      901 ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa
      961 aaataatgag gtcattctta gtggagaaat ccctgctcgg tgtattcaag aatatcgtag
     1021 tgatttaact ttctttacaa atggacgtag tgtttgttta acagagttaa aagggtacca
     1081 tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga tagataaagt
     1141 acgatatatg ttcaataaaa taacttagtg tattttatgt tgttatataa atatggtttc
     1201 ttgttaaata agatgaaacc cgtggtgcta gttaattttt tcctagagga aaatg
//
LOCUS       DASHFF010000240         1136 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.191, whole genome shotgun sequence.
ACCESSION   DASHFF010000240 DASHFF010000000
VERSION     DASHFF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.191"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            266..1120
                     /locus_tag="U2I75_003016"
                     /note="aph(2'')-Ia"
     CDS             266..1120
                     /locus_tag="U2I75_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HEN1698042.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IESVKYFV"
BASE COUNT          477 a           91 c          188 g          380 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccgt acaagatata ttaaattgat
       61 ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa
      121 taatccaaga gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc
      181 agaacatgaa ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga
      241 tgataatgcc acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt
      301 caaagtagat agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa
      361 taatgaatac atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga
      421 aaaagcaata tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat
      481 tgaatattcg tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac
      541 ttttttaaca ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg
      601 agatattgcc agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg
      661 tactattgat aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta
      721 taatgattta actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc
      781 aacaacagtt tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct
      841 attgttagat ggcaataata gattaactgg aataattgat tttggagatt ctggaattat
      901 agatgaatat tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa
      961 ttttggagaa gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata
     1021 tcaagatata gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat
     1081 taaacaggaa tttatcgaga gtgtaaaata ttttgtgtaa atgaaaaaat ccatac
//
LOCUS       DASHFF010000241          981 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.197, whole genome shotgun sequence.
ACCESSION   DASHFF010000241 DASHFF010000000
VERSION     DASHFF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.197"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            87..146
                     /gene="ermCL"
                     /locus_tag="U2I75_003017"
     CDS             87..146
                     /gene="ermCL"
                     /locus_tag="U2I75_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HEN1698043.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            206..940
                     /locus_tag="U2I75_003018"
     CDS             206..940
                     /locus_tag="U2I75_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HEN1698044.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          404 a          120 c          133 g          324 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca gcttgtgcta taattattct
       61 aatttcataa aaaggagaat taatatatgg gcatttttag tatttttgta atcaacacag
      121 ttcattatca accaaacaaa aaataagtgg ttataatgga ttgttaatat aaacattcat
      181 tataacctca taggagtggg ttataatgaa caaaaaaaat ataaaagata gtcaaaactt
      241 tattacttca aagcatcata taaatgaaat tttgagaaat gtacatttaa atacaaatga
      301 taatattatt gagattggtt cagggaaagg tcatttcacg tttgaattag ctaaaaggtg
      361 taattatgta accgccattg aaatagatcc taaattatgt aggataacta aaaacaaact
      421 tattgaatat gagaacttcc aggttatcaa taaagatatt ttacaattta agtttcctaa
      481 aaataagtca tataagatat atggaaatat accctacaat ataagtacag atataattcg
      541 aaaaattgtt tttgaaagca cagctacaga aagttattta atagtggaat atggatttgc
      601 taaaaggttg ctaaatacaa atcgttcact agcactattt ttaatgacag aagttgatat
      661 atccatatta agtaaaatcc ctagagaata ctttcatcca aaacctagag ttaatagctc
      721 gttaattgta ttaaaaagac acccttcaaa aatatcactc aaagatagaa aacaatatga
      781 aaattttgtt atgaaatggg ttaacaaaga atacataaaa ctattttcca aaaaccaatt
      841 ttatcaagcc ttaaaatatg caagaattga cgatttaaac aatattagct ttgaacaatt
      901 cttgtctctt ttcaatagct ataaattatt taatagataa gttaggctct ttgtcaaatg
      961 atgttggtaa agagaaatct t
//
LOCUS       DASHFF010000242          855 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.206, whole genome shotgun sequence.
ACCESSION   DASHFF010000242 DASHFF010000000
VERSION     DASHFF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.206"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            242..790
                     /gene="aac(6')"
                     /locus_tag="U2I75_003019"
     CDS             242..790
                     /gene="aac(6')"
                     /locus_tag="U2I75_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1698045.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          306 a          126 c          180 g          243 t
ORIGIN      
        1 tttttttaaa gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat
       61 tgatggcttt cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt
      121 tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag
      181 cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg
      241 tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt
      301 actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat
      361 gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat
      421 tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag
      481 ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc
      541 ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt
      601 aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg
      661 tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa
      721 tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa
      781 ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat
      841 ttacgaaaat tactt
//
LOCUS       DASHFF010000243          828 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37981
            SAMN38764606-rid20548913.guided.207, whole genome shotgun sequence.
ACCESSION   DASHFF010000243 DASHFF010000000
VERSION     DASHFF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764606
            Sequence Read Archive: SRR27177228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024605.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024605.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:11:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,953
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 13 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764606-rid20548913.guided.207"
                     /strain="VRE37981"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(39..341)
                     /locus_tag="U2I75_003020"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="U2I75_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          285 a          154 c          102 g          287 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccat
//
